LOCUS       DATGDI010000001         2547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_349094, whole genome shotgun sequence.
ACCESSION   DATGDI010000001 DATGDI010000000
VERSION     DATGDI010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349094"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2355)
                     /locus_tag="VKO45_00005"
     CDS             complement(<1..2355)
                     /locus_tag="VKO45_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMA04294.1"
                     /translation="MHEPPVKKVLYWCDRCSVPLIARTCGCGAEGRRVLLQAPYDVRP
                     ALAADLALLRGLVEDRFGTVPLPKIVLLNKAGGIDRNDLVIIHGERFGWLSFDPLTRR
                     SRFDLAPGGLPFVVGHATRGIVDLGLAAAAGDLEGRRIGGKRIPVRTGEQDGTVVVKY
                     RNGHGTGVLREGQIRVREMVAVETRSPPDPEWGVAIDRNRHHLKNLERNAIREIRHHM
                     GDRPCANVSFSGGKDSMAAMALARKAGIPSAFFIDTGIEFPETVRFVEEQGVEVIRKA
                     GDFWAAVEKAGPPAKDMRWCCKLLKLQPLKLHLATTGPCVTVQGNRWYESWNRADLEA
                     TSQNPANPLQLNISPIRNWRALEVFLYLWWRKLPVNPLYDRGIERIGCYLCPAMLESE
                     HELIREMHPEFAQRWDRFLERWAAGHNLPEEYARWGLWRWRGLPPKMQELCARGGIAL
                     EGNRLRHVPREPPGRERPAPPREMAKVTPVPGEPIRKDFPLIADLVYLDSAATGISPE
                     PVLEAMLGYERGYRANVGRGVHRLSQVASQKYWDAHQKVKGFIGAKEGEVVFTRDTTE
                     AIAMVARGLTWKRGDRVVTTVLEEDSNFVPWLRLRERGVECDILPLAKEEILDLSRLG
                     ELITDGTRLVAVTHVSGVLGSINPIREIARTCRDHGALLLVDGSHSVPHMPVDVSILG
                     CDFLCLSGDRMLGPTGTGVLWMKDPSLEPLHVGGGMVGTVSSLGYTLAEGYQRYEAGT
                     PNVGGGIGLGAAVDYLTGIGMDRIRAHEMCLAGRLIEELRNLPGI"
     gene            2350..>2547
                     /locus_tag="VKO45_00010"
     CDS             2350..>2547
                     /locus_tag="VKO45_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04295.1"
                     /translation="MHDSYLWVIPAYKNSAGYSKHPAGSLIRILTSCERGRTIYSRGI
                     LFTAGKGSEDDGFARYGRGDDR"
BASE COUNT          426 a          885 c          810 g          426 t
ORIGIN      
        1 tatccccggc agattccgga gctcctcgat cagccggccg gcaaggcaca tctcgtgggc
       61 ccggatacgg tccatcccga tcccggtcag gtaatccacc gcggccccga ggccgatccc
      121 gcccccgacg ttcggcgttc cagcctcgta gcgctggtac ccctcggcga gggtataccc
      181 caaggaggat acggtcccga ccattccccc gccgacgtgg agcggttcga gcgagggatc
      241 cttcatccag agcacgcctg tccccgtggg cccgagcatc cgatccccgg agaggcagag
      301 gaagtcgcac cccaggatcg agacgtcgac cggcatgtgc gggaccgagt gactcccgtc
      361 cacgaggagg agcgccccat ggtcccggca ggtgcgggcg atctcccgga tagggttgat
      421 gctcccgagc actcccgaaa catgggtgac cgcgacgagg cgcgtcccat cggtgatgag
      481 ttccccgagc cgtgagagat cgaggatttc ctccttcgcg aggggcagga tgtcgcattc
      541 caccccccgc tcccgcaggc ggagccaggg cacgaaattc gaatcctcct cgagcacggt
      601 ggtgacgacc cggtctcccc tcttccaggt gagcccgcgg gcgaccatag cgatggcctc
      661 ggtcgtatcc ctggtgaaga caacctcccc ctccttcgcg ccgatgaacc ccttcacctt
      721 ctggtgggca tcccagtact tctgggaggc gacctgggag agccggtgca cccctctccc
      781 gacattcgcc cggtacccgc gctcgtaccc gagcatggcc tcgagcaccg gctcggggga
      841 gatgcccgtt gccgcactgt cgaggtagac gagatcggcg atgaggggga agtccttccg
      901 gatgggttcg ccgggcacgg gggtaacctt cgccatctcc cgcggtgggg cgggcctctc
      961 acggccgggt ggttcacggg gaacgtgcct cagccgattc ccctcaaggg cgatcccgcc
     1021 cctcgcgcag agctcctgca tcttcggggg gagtcccctc cagcgccaga gcccccaccg
     1081 ggcatactcc tccgggaggt tgtggcccgc ggcccacctc tccaggaacc ggtcccacct
     1141 ttgcgcaaat tcggggtgca tctccctgat cagctcgtgc tcgctctcga gcatcgccgg
     1201 gcagaggtag catccgatcc tctcgatgcc ccggtcgtaa agcgggttca cggggagctt
     1261 ccgccaccag aggtacagga aaacctcgag ggcccgccag ttgcggatcg gggagatgtt
     1321 cagctggagg ggattcgccg ggttctggct cgtggcctcg agatcggcac ggttccagga
     1381 ctcgtaccac cggttcccct gcaccgtgac gcacgggccc gtggtcgcga gatggagctt
     1441 caggggctgg agcttcagga gcttgcagca ccaccgcatg tccttggcag ggggacccgc
     1501 cttctccacc gcggcccaga agtccccggc tttccggatc acctcgaccc cctgctcctc
     1561 gacgaacctc acggtctcgg ggaactcgat gcccgtgtcg atgaagaagg ccgaggggat
     1621 ccccgccttc cgggcaaggg ccatcgccgc catggaatcc ttgccgcccg agaaggagac
     1681 attggcacac gggcgatcgc ccatgtgatg cctgatctcc cggatggcat tcctctcgag
     1741 gtttttcagg tggtgcctgt tccggtcgat ggcgacaccc cactccgggt caggagggct
     1801 ccgggtctct actgccacca tctctctcac ccggatctgc ccttccctca acactccggt
     1861 accgtgccca ttgcggtact tcaccaccac cgtcccgtcc tgctcgcccg tcctgacagg
     1921 gatccgtttg ccgccgatcc ggcgcccctc cagatctcct gccgccgccg cgaggccgag
     1981 atccacgatc ccccgggtcg cgtggcccac gacgaagggg aggcctcccg gggcgagatc
     2041 gaacctggac ctccgggtca ggggatcgaa ggagagccag ccgaagcgct ccccgtggat
     2101 gatgacgagg tcgttcctgt cgatgccccc tgccttgttc aggaggacga tcttcgggag
     2161 cgggaccgtc ccgaaccggt cctccacgag cccccggagg agggccaggt cggcggcaag
     2221 ggccggccgg acatcgtacg gtgcctggag gaggacccgc ctcccctccg ccccgcagcc
     2281 gcaggtccgg gcgatgaggg gcacgctgca gcgatcgcac cagtagagga ccttcttgac
     2341 cggcggctcg tgcatgacag ctacctgtgg gtgataccgg cctataagaa cagcgcgggg
     2401 tattcgaaac acccggcggg aagcctcata cgtattctca cttcctgcga gagaggcaga
     2461 accatatata gcaggggaat cctgtttact gccgggaaag gcagtgaaga cgacggattc
     2521 gcacgatacg ggaggggaga tgaccgg
//
LOCUS       DATGDI010000002        11863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_203664, whole genome shotgun sequence.
ACCESSION   DATGDI010000002 DATGDI010000000
VERSION     DATGDI010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 11863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11863
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203664"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..622
                     /locus_tag="VKO45_00015"
     CDS             182..622
                     /locus_tag="VKO45_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HMA04296.1"
                     /translation="MERTLGFKERRYIEELRILTKSTAVDCLIDERFDRIIYVIRPGD
                     MGLAIGKKGENIRRMEKVLGKKIEMVEYSENPDDFVANMFRPAVLSAVRRDEGGRVNI
                     ILQKKGDLGIAIGKGGANVEKARLLFRRFFGSELGEILSAGDGS"
     gene            619..930
                     /gene="hisE"
                     /locus_tag="VKO45_00020"
     CDS             619..930
                     /gene="hisE"
                     /locus_tag="VKO45_00020"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HMA04297.1"
                     /translation="MKHLDVIPELWEVLRDRLAHPREGSYVNRLARGEKGIDGILEKV
                     GEEAAEFIIAVKNGERRDIVWEAADLEFHLLLALAAAGVDLEEVLSELEKRRHPEKGK
                     V"
     gene            complement(939..1406)
                     /locus_tag="VKO45_00025"
     CDS             complement(939..1406)
                     /locus_tag="VKO45_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HMA04298.1"
                     /translation="MILTIAMDERECRVRGVFLSMSGQGAAAPTVVLELPGGDCIPIY
                     IGLWEAISIRNAIKGEVLPRPITHDLFVEVLRRLHATVGGLSIDSLEDGIFYAKLRVK
                     LDGMVEEVDCRPSDGIAIALRTHAPITVGEGVVSQASKKREDLPELQDLDSVV"
     gene            complement(1413..2456)
                     /locus_tag="VKO45_00030"
     CDS             complement(1413..2456)
                     /locus_tag="VKO45_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HMA04299.1"
                     /translation="MERSQWKWLIVSVSFSLVVLALVLYFTVTERTFEYLKRLNPLFL
                     FLAIVFHFLAYGFWAMRIRTMAASLGHRVEFTYCLNLVMANLLAGAITPAQAGGEPVR
                     IHQLYRNNVPLGDATAIVLVERVLDAVILGVAGIGAIFLLGEMVEAVPRGLTLVITLA
                     WLAMVVIIGAFVFSVRNPEFLKRSLKRISGVFIRRWDFTRVERLIASIDREVDNFHDS
                     LSRFMAHGRRGLLWGALYTGLFWFSEFIIVSLILIGIGEQPYYLLSLIAQIVIAIVMM
                     IPLTPGSSGVAELSATSIYGLFINTSIVGVLVVVWRAIFYYLNIAVGIVASIPLIQRE
                     MLRRDGEPPEGLT"
     gene            complement(2569..3147)
                     /locus_tag="VKO45_00035"
     CDS             complement(2569..3147)
                     /locus_tag="VKO45_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF357 domain-containing protein"
                     /protein_id="HMA04300.1"
                     /translation="MKIGDYGESLGRHLSGISPSAPPGTALFRVAGEAIEMAEAYLLD
                     GRSFLSAGDLMNGLAAFTYAAGWLDASASLGLFGAVTALGIPVFAEEAGSLGGDRVRE
                     KRTRYRRMLSGAIEALECSPDPGSPLYPVAAAILGRAETLLGEGDLLAGQGREGDSLA
                     CYSHGHGWLDAGVRAGLFRILHSRDLFTVGPL"
     gene            complement(3134..3895)
                     /gene="dph5"
                     /locus_tag="VKO45_00040"
     CDS             complement(3134..3895)
                     /gene="dph5"
                     /locus_tag="VKO45_00040"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HMA04301.1"
                     /translation="MLTFIGLGLYDERDLSVKGLEAVRSADTVYLETHTSRLTGTTLP
                     SMERFYGRPVIPLTREEMEVDPGEILARAKAGNVALLCGGDPMVSTTHADLRLRAHAA
                     GVETRIIHGASIQTAVSGLTGLQNYRFGRSTSIPFPSGSWFPTTPLETVLANLGLRLH
                     TLVFLDIQADRSMTVGEGIALLGEMAHRISRPAPGFYVGVARAGSADAFVAAGGLAWL
                     ASQDFGPPLHVLVVPADRHPVEQEYLEAFTRYENR"
     gene            4055..5746
                     /gene="polX"
                     /locus_tag="VKO45_00045"
     CDS             4055..5746
                     /gene="polX"
                     /locus_tag="VKO45_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HMA04302.1"
                     /translation="MAYSNREIAERLRFMGQLLEVTGEEDVFKVRAYERAAKHLEDLT
                     TPVSDMDEKALLAIPGIGSKIAGKIASILKTGTFPELEELEGRIPESLPELLKLEGVG
                     PKTVHTLWMKLGIQSVDDLERAARQRRIRAVKGFGEKKESEFLKSIARYRGSTTRMTR
                     DQADAVVAKATSVLSPGQFEVAGSYRRGKSTVGDIDIASVESPLAVNSRVRSIADEVI
                     AAGSEKTSIRCLGHQVDFRFTEPGSFGSMLLYLTGSKEFNIHLREIAIGKGLRLNEYG
                     LEEREGGGLHTFPDEASLFSFLGLPYFPPEIREDRGEIERALAGDLPVLVEEKAIRGD
                     LHIHTTATDGHLTLGEFAAAGTELGYEYLLVSDHSSTLGITHGLDADGVKAQGKEIER
                     VNRGSGCQLLAGIEVDILADGTLGLPDRVLADLDLVIASVHSALNEEKDVMTRRILTA
                     VSNEHVDILGHPTGRLFPSRPASQVDMDRVIEAAKAAGTALEINASPFRMDLDDPQIR
                     EVRDRGVKLSIGTDAHSRPELGHIRHGVTLARRGWCAPGDILNTLGREALLGWAS"
     gene            5743..6333
                     /locus_tag="VKO45_00050"
     CDS             5743..6333
                     /locus_tag="VKO45_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HMA04303.1"
                     /translation="MIYWIYERLLLRRLTLFPEHLCFMITEQDMVEAPGKIAEVSRWC
                     RDLGIHGVTFHIGTQNPARIDPFLPFICGIANFARLELHGGEREEVMGEGMPVHVAIG
                     HSGREEITRCIRRMAEAGVDPGRVDEDLIDSYLTFRYTPDLVIKTGASHLTDFLIWQS
                     VYAELFFTDVNWRLLRKVDFLRALRDYQSRKRRFGA"
     gene            complement(6377..7159)
                     /gene="uppS"
                     /locus_tag="VKO45_00055"
     CDS             complement(6377..7159)
                     /gene="uppS"
                     /locus_tag="VKO45_00055"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HMA04304.1"
                     /translation="MKARSGLEGLYERLLGWQIRHVPLHIAIIQDGNRRFARSQGMDA
                     SEGHRAGADTTEKVLDWAHEMGVKYITLYSFSTENFRRDSAELAGLFTLFKERFEKII
                     SDERIHSRRIRVRVFGDRTLLPPDLLERVEAAEEATRSYGNYFLNVALAYGGRNEIVH
                     AARSILRDVERGSLSPDEIDPALVEHHLYDGKYLPPVDLIIRTGNERRTSNFLPWLAN
                     GKESAVYFCAPHWPAFRKIDFYRALRVYDQRMRGKGMAWNEA"
     gene            complement(7156..8145)
                     /locus_tag="VKO45_00060"
     CDS             complement(7156..8145)
                     /locus_tag="VKO45_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA04305.1"
                     /translation="MSSKGVAARGCELCRLGAKLVLFVTGLCHRECWYCPLSEERAGR
                     DLAFANDRPVEGPEDFLAEARMMGALGTSITGGEPFLVPDRVTRFCTVLKEAFGKAHH
                     IHLYTGMAPTREMLLPLAGLVDEIRLHPPPESWPAIGSSAYARAIGEARSLGFAVGIE
                     VPALKGLPALAPVLPEIDFLNINELEWGGINADEMRKRGYPPADDIHNAVKGSRRWAA
                     DLPHREKVRWCSSRFKDAVQLRERLKRIAARTARSFDEVTGDGTILYGVIEPDGSLPL
                     SRFGLPGGSYEECDGRLELSWRTLRKRAGKLPGKKYIVERYPNRGVVVEVIPL"
     gene            complement(8135..9664)
                     /gene="lysS"
                     /locus_tag="VKO45_00065"
     CDS             complement(8135..9664)
                     /gene="lysS"
                     /locus_tag="VKO45_00065"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HMA04306.1"
                     /translation="MGGASERIHWADVVAASVREGQEHRIATGITPSGPIHIGNMREV
                     LTGDLVYKAMGERGLPVELLYIADDFDPLRKVYPFLPESYAAYVGKPISDIPCPCGEH
                     RSYADHFLTPFLSALSELDISPRVYLGSELYRSGAYTKEIGIALEHTGEIRHILEQVS
                     GRTLPPDWAPFYPVCSVCGKLSPARILEHDPSRHTVRYRCACGNEGTSDYSKGEGKLV
                     WRVDWPMRWAHFGITVEPFGKDHATAGGSYDTGKEIARRVFRVEPPHPVIYEWISLKG
                     KGAMHSSKGVAVTISEMLEIVPPDVLRYLIARTRPERTIDFDPGMGLISLIDEYDRVS
                     EKGDGREYELSRIASIPTRIPFRHMVTVVQIAHDDRRLFQCLARTSYDVTDRENILKQ
                     AERVRVWIRKYAPESVRFSVKERLPLQIGDLSEKDIQALSAYLEFVKALPAWSAEAIH
                     NGVYEVAPRCGTDAKRLFELVYLAFLGQSRGPRLGWFLEALGREFVTGRLSEAVYPCV
                     L"
     gene            9912..>11863
                     /locus_tag="VKO45_00070"
     CDS             9912..>11863
                     /locus_tag="VKO45_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04307.1"
                     /translation="MKPGEMRKPGRKARGINEVVGLLLILAVVIMTITVYMIYFMPTL
                     GRDQEISQMSEVKERFAEFKLNIDSLWMTRQGTSSFGPSLSLGSGETTGLLSFFPFLR
                     PPRAGAVLALNQRDENITITSDSYILGGGGSKVGQAVGTSPVLLSVNTTPSNLYIKLY
                     ATDLLKERGVFVHGPTWDAWVNVTPNYIYTDPTSWTLNGTDITVTTLVGGRAVATSLP
                     VYRSISSSTTYKVDLMNPVYGISPQFQNPQNLWLSASDGSGILASYEMQNSYTPMVSA
                     STFPLGSIEFRSNNLYYTPQTHYYQLGGVFLEQDEGSTTEIPPDISVSLSNGAPVVNV
                     GEILLQGNAVTANMSGSGPITVTSQVTGIVNAPLMPGKNTRWVNLRIQAANTNAADMW
                     NRTFHEIAARGGLPAGSYAAGSSGTIAYLNITGDPQLYTIQLSLTRVNIATDYILEYS
                     PGGIARGWREGPGFPGQPGTDLTGCGDGTCNGAETCTTCPGDCGICPTCGDGTCNIGE
                     TCSSCPGDCGACPPSCGDLLCNGGETCSSCPGDCGACPTCGDLICNGAETCSSCPGDC
                     GACGGGGGGGGAGPLVISPCDWVSRKKLTINGTMVSGTQTNFPVLVSLPFDSDLAASA
                     QANGNDIVFTDSGGSTVLPHEIENFT"
BASE COUNT         2133 a         3955 c         3858 g         1917 t
ORIGIN      
        1 caagtcctga ggggccccaa acccccctgg acagctccgg ctccttcgga cccgtcgctc
       61 aagtcctgag gggccccaaa cccccctgga cagctccggc tccttcggac ccgtcgctcc
      121 ctcggacccg gggctcattt ctgtaaggat atatacgggg agcgcattag gatcacctga
      181 aatggagcgg accctcggtt tcaaggagag acggtacatc gaggagctca ggatcctcac
      241 caaatccacc gccgtggact gcctcatcga cgagcggttc gaccggatca tctacgtgat
      301 ccggcccggc gacatgggcc tcgccatcgg gaagaaaggg gagaacatca ggaggatgga
      361 gaaggttctc gggaagaaga tcgagatggt ggagtactcg gagaacccgg atgacttcgt
      421 ggcgaacatg ttccggccgg cggtcctttc ggcggtccgg agggacgagg gcgggagggt
      481 gaacatcatc ctccagaaga agggggatct cgggatcgcc atcggcaagg gaggggcgaa
      541 cgtggagaag gcccggctcc tcttccggag gtttttcggg tccgaactcg gagagatcct
      601 gtcagcgggg gatggctcgt gaagcatctc gacgtgatcc ccgagctctg ggaggtcctc
      661 cgggaccggc tcgcccaccc ccgggaggga tcgtacgtga accggctcgc ccggggtgag
      721 aagggaatcg acgggatcct cgagaaggtg ggggaggagg cggcggagtt catcatcgcg
      781 gtgaagaacg gggagaggcg ggacatcgtg tgggaggcgg ccgatctcga gttccacctc
      841 ctcctcgccc tcgccgcggc gggggtggat ctcgaggagg ttctctccga gctcgagaag
      901 aggcgccacc cggaaaaggg gaaggtctag aacctggcct acaccactga gtcgaggtcc
      961 tgcagttcgg ggaggtcctc ccgtttcttg gaggcctggg agaccactcc ctcccccacg
     1021 gtgatgggcg catgggtcct gagggcgatg gcgatgccgt cgctcggccg gcagtccacc
     1081 tcctcgacca tcccatcgag cttcacccgg agcttcgcgt agaagattcc gtcctcaagg
     1141 gaatcgatgg agaggccgcc gaccgtcgcg tggagcctcc ggagcacctc cacgaagagg
     1201 tcgtgggtga tgggccgggg gagcacctcc cccttgatgg cgttccggat ggagatggcc
     1261 tcccacaggc cgatgtagat ggggatgcag tccccgcccg gaagctcgag cacgacggtg
     1321 ggggccgcgg ctccctgccc gctcatcgaa aggaagaccc cccggacccg gcactcgcgc
     1381 tcgtccatgg cgatggtaag aatcaccctc ccctacgtta aaccttcggg tggctccccg
     1441 tcgcggcgca gcatctcccg ctggatgagg gggatggagg cgacgatccc cactgcgata
     1501 ttcaggtagt agaagatggc cctccagacc acgacgagga ccccgacgat ggaggtgttg
     1561 atgaagaggc cgtagatgga ggtcgccgag agctcggcga cccccgagga tccgggggtg
     1621 agggggatca tcatgacgat ggcgatgacg atctgggcaa tgagggacag gaggtagtag
     1681 ggctgctccc cgatgcctat gaggatgagg gagacgatga tgaactccga gaaccagaag
     1741 agcccggtgt agagagctcc ccagaggagc ccccgacgcc cgtgggccat gaaccgggag
     1801 aggctgtcat ggaagttgtc cacctcccgg tcgatggagg cgatgaggcg ctcgaccctc
     1861 gtgaagtccc agcgcctgat gaagaccccc gagatcctct ttaaggacct cttcaggaac
     1921 tcggggttcc tcaccgaaaa gacgaaggcc ccgatgatga cgaccatcgc gagccaggcg
     1981 agggtgatca cgagggtgag gcccctcggg accgcctcca ccatctcccc gaggaggaag
     2041 atggccccga tccccgcgac cccgaggatg acggcatcga gaacacgctc gacgaggacg
     2101 atggccgtgg catcccccag ggggacgttg ttccggtaga gctggtgaat tcggaccggt
     2161 tccccgccgg cctgggcggg ggtgatggcc ccggcgagga ggttcgccat gaccaggttc
     2221 aggcagtagg tgaactcaac ccggtgcccg agagaggcag ccatggtgcg aatcctcatc
     2281 gcccagaacc cgtaggcgag gaaatggaac acgatggcga ggaagaggaa aagggggttc
     2341 aggcgtttga ggtactcgaa ggtccgctcg gttaccgtga agtagaggac gagggcgagg
     2401 accaccaggc tgaagctcac cgagacgatg agccacttcc actgggaacg ctccacgata
     2461 acctcctcat ccttcccccg ggaacgcgtt tcgccccttg aggtgcaaat gtaccggtta
     2521 cagggaaagc tcataacctt ttagaggggg cccagagaaa gtccctttct ataaagggcc
     2581 caccgtgaac aggtcccggg aatggaggat ccggaagagc ccggccctga ccccggcgtc
     2641 aagccagcca tggccatggc tgtagcatgc gagagaatcc ccttcccttc cctgcccggc
     2701 caggaggtcc ccctccccga ggagggtctc cgcccgcccg aggatggccg cggccacggg
     2761 atagaggggg cttcccgggt cgggggaaca ttcgagggcc tcgatggcgc cggagagcat
     2821 ccgccggtac cgggtcctct tctcccggac ccggtccccc ccaagggatc cggcctcctc
     2881 ggcgaagacc gggatgccca gggccgtcac cgccccgaag agcccgaggg aggccgaggc
     2941 gtccagccag ccggcggcgt aggtaaaagc ggcgagcccg ttcatcaggt caccggccga
     3001 gaggaagctc ctcccgtcca ggaggtacgc ctccgccatc tcaatcgcct ccccggccac
     3061 ccggaaaagg gcggtccccg gcggggctga tggggagatc cccgagagat gcctccccag
     3121 cgactccccg tagtcaccga ttttcatacc gggtgaaggc ctccaggtat tcctgttcca
     3181 ccgggtgccg gtcggccggg accacgagga cgtggagggg cgggccgaag tcctgcgacg
     3241 cgagccatgc caggccacca gcggccacga aggcatccgc cgatcccgcc cgggcgaccc
     3301 ccacgtagaa accgggggcc ggcctcgaga tccggtgggc catttcaccg agaagggcga
     3361 tcccctcccc cacggtcatg gagcgatcgg cctggatgtc caggaagacg agggtgtgga
     3421 gccggaggcc gaggtttgcg aggaccgtct cgaggggcgt cgtcggaaac caggagcccg
     3481 acgggaaggg gatggaggtc gatctcccga accggtagtt ctggaggccg gtgaggccgg
     3541 agacggcggt ctggatggat gccccgtgga tgatccgggt ctccacgccc gcggcatggg
     3601 cccggagccg gagatcggca tgagtcgtgg agaccatggg gtcccccccg cagaggaggg
     3661 ccacgttccc cgccttcgcc cgggccagga tctcgccggg gtccacctcc atctcctccc
     3721 tcgtgagggg gatgaccggt ctcccgtaga acctttccat cgagggaagg gtcgttcccg
     3781 tgagccggga ggtatgggtc tcgaggtaca cggtgtccgc cgaccggacg gcctcgaggc
     3841 ccttcaccga gaggtctctc tcgtcataga ggccgaggcc gatgaaggtg agcatgggtc
     3901 cgcaggtaac tatgcgaggg catgattaat tattgccccc atcctgtccc ctctctctgc
     3961 ggtcgcatcc tcaggtccaa aagacccgga tcaggctcgc ggatgtggcg agtggaacga
     4021 gagaaccata taccgcgggg gacgagatgt acgcatggca tactcgaacc gggagattgc
     4081 ggagcgtctc cggttcatgg gccaactcct ggaggtgacc ggcgaggagg acgtgttcaa
     4141 ggtgcgcgcc tatgaacgcg ccgcgaagca cctggaggat ctcaccacgc ccgtctcgga
     4201 catggatgag aaggcgctcc tcgccatccc cggcatcggg agcaagatcg ccggaaagat
     4261 cgcctccatc ctgaagaccg ggaccttccc ggagctcgag gaactcgagg gaaggatccc
     4321 cgagtccctc ccggagctcc tgaagctcga gggggtgggc cccaagaccg tccacaccct
     4381 ctggatgaag ctcgggatcc agtccgtcga tgacctggag cgggcggccc ggcagcggcg
     4441 gatccgggcg gtgaagggct tcggagagaa gaaggagtcc gagttcctca agtccattgc
     4501 ccggtaccgc ggctcgacga cccggatgac ccgggaccag gccgatgccg tggtggcaaa
     4561 ggcaacctcc gttctctccc cggggcagtt cgaggtggcg gggagctacc ggcgggggaa
     4621 atccaccgtc ggggacatcg acatcgcctc cgtggaatcg cccctggcgg tgaactcccg
     4681 ggtcaggtcc atcgcggacg aggtgatcgc ggcggggagc gagaagacct ccatccggtg
     4741 cctgggccac caggtggatt tcaggttcac cgagcccgga tccttcggct ccatgctcct
     4801 gtacctcacc ggttccaagg agttcaacat ccacctgcgg gagatcgcca tcgggaaggg
     4861 cctccggctg aacgagtacg gcctcgagga gcgcgagggg ggcggcctcc acaccttccc
     4921 tgacgaggcc tccctgttct ctttcctcgg ccttccctat ttcccgccgg agatccgcga
     4981 ggaccggggg gagatcgagc gggcgctcgc cggggatctc cccgttctcg tggaggagaa
     5041 ggccatccgg ggcgacctcc acatccacac gacggcgacc gacgggcacc tgaccctcgg
     5101 ggagttcgcc gccgcgggaa ccgagctcgg gtacgagtac ctcctcgtgt ctgaccactc
     5161 ctcgaccctg gggatcaccc acggcctgga tgccgacggg gtgaaggccc aggggaagga
     5221 gatagagcgg gtgaaccggg gatccggctg ccagctcctg gccgggatcg aggtggacat
     5281 cctggccgac ggtaccctgg ggctcccgga ccgggtcctc gccgatctcg acctcgtcat
     5341 cgcctcggtc cattcggcgc tcaacgagga gaaggacgtc atgacccgcc ggatcctcac
     5401 ggccgtctcc aacgagcatg tggacatcct gggccatccg acgggccggc tcttcccctc
     5461 ccggccggcc tcccaggtgg atatggaccg ggtcatcgag gcggcaaagg ccgcggggac
     5521 ggccctcgag atcaacgcct cccccttccg gatggacctc gacgatcccc agatccggga
     5581 ggttcgggac cggggcgtga agctttccat cgggacggac gcccattccc ggccggaact
     5641 cgggcatatc cggcacgggg tcaccctcgc ccggcggggc tggtgcgcac ccggggatat
     5701 cctgaacacc ctcggccggg aggcgctgct cgggtgggcc tcatgatcta ctggatctac
     5761 gagaggctgc tcctccgcag gctcaccctt ttccccgagc acctctgttt catgatcacg
     5821 gagcaggaca tggtggaggc acccgggaag atcgccgagg tgagccggtg gtgccgcgat
     5881 ctcgggatcc acggggtcac cttccacatc ggcacccaga acccggcgcg gatcgacccg
     5941 ttcctcccct tcatctgcgg gatcgccaac ttcgcccgcc tcgagctcca cggaggggag
     6001 agggaggagg tcatgggcga ggggatgccc gtccatgtcg ccatcggcca ttcaggccgc
     6061 gaggagatca cccggtgcat ccggcggatg gcggaggccg gggtggaccc cgggagagtg
     6121 gatgaagatc tcatcgactc gtacctcacc ttcaggtaca ccccggatct cgtaatcaag
     6181 acgggcgcct cccacctgac cgatttcctc atctggcaga gcgtgtacgc ggagctcttc
     6241 ttcaccgacg tcaactggag gctcctccgg aaggtggact tcctgcgggc tctcagggac
     6301 taccagtccc ggaagcgccg gttcggtgcc tagcgacgga ccgctcacgg tccggagcga
     6361 ggccgaacca tcaggttcag gcctcattcc aggccatgcc cttcccgcgc atccgctgat
     6421 cgtatacacg cagggcccgg tagaagtcga tcttacggaa ggccggccag tgtggggcgc
     6481 agaagtagac cgcgctctcc ttcccgttcg cgagccaggg aaggaagttg gaggtgcggc
     6541 gctcgttccc ggtgcggatg atgaggtcca cgggggggag gtacttcccg tcgtagaggt
     6601 ggtgctccac gagggccggg tcgatctcat ccgggctgag agagcccctt tccacgtcgc
     6661 ggaggatgga gcgggccgcg tggacgatct cgttcctccc cccataggca agggcgacat
     6721 tcaggaagta gttcccgtag gaccgggtgg cttcctcggc ggcctccacc ctttccagga
     6781 gatcgggcgg gaggagggtg cgatccccga agacccgcac ccggatcctc cggctgtgga
     6841 tcctctcatc tgagatgatc ttctcgaacc tctccttgaa gagagtgaag aggcccgcga
     6901 gctccgcgct gtcccgccgg aagttctcgg tggagaagga gtagagggtg atgtacttca
     6961 cccccatctc gtgggcccag tcaagcacct tctcggtggt gtcggccccg gcccggtgcc
     7021 cctccgatgc gtccatgccc tgggacctcg cgaaccggcg gttcccgtcc tggatgatgg
     7081 cgatgtgaag cgggacgtgc cggatctgcc agccgaggag acgctcgtac agcccctcga
     7141 ggcccgagcg ggccttcaca gggggatcac ctccaccacc accccgcgat tcgggtagcg
     7201 ctccacgatg tacttcttcc cggggagttt tcccgcccgt ttccggagcg tccgccagga
     7261 gagctcgagg cgtccgtcgc actcctcgta gcttcccccg ggaagtccga aacgggagag
     7321 ggggagggac ccgtcgggct cgatgacccc gtagaggatg gtcccgtcac cggtgacctc
     7381 atcgaaggaa cgggcggtcc gggcggcgat gcgcttcagg cgctcccgga gctggaccgc
     7441 gtccttgaac ctcgatgagc accacctgac cttctcacgg tggggaagat ccgctgccca
     7501 gcgtcgcgag cccttcaccg cgttgtgaat gtcgtcggca gggggatatc cccgtttcct
     7561 catctcatcg gcattgatcc cgccccattc gagctcgttg atgttcagga agtcgatctc
     7621 cgggaggaca ggggcaaggg cggggaggcc cttcagggcg gggacctcga tcccgaccgc
     7681 gaatccgagg gagcgggcct ccccgatggc ccgggcatac gccgatgacc cgatggcggg
     7741 ccaggactcc ggcggcgggt ggaggcggat ctcgtccacg agtcccgcga gggggagaag
     7801 catctcccgg gtcggggcca ttcccgtgta caggtgaatg tggtgggcct ttccaaaggc
     7861 ctccttcagg accgtgcaga accgggtcac ccggtcgggc acgaggaagg gctcaccgcc
     7921 ggtgatgctc gttccgaggg ctcccatcat ccgggcctcg gcaaggaaat cctccggccc
     7981 ctccaccggc cggtcgttcg cgaaggcgag gtcccggccg gccctctctt cggagagcgg
     8041 gcagtaccag cactcacggt ggcagaggcc cgtgacgaag aggaccaact tggcccccag
     8101 ccggcagagc tcgcaccccc gtgcggccac ccctttagag gacacaggga tacaccgcct
     8161 cggagaggcg cccggtcacg aactccctcc cgagggcctc gaggaaccag cccaggcgcg
     8221 ggccccgcga ctgcccgagg aaggcgaggt acaccagctc gaagagcctc ttcgcgtccg
     8281 ttccgcaccg gggcgccacc tcgtacaccc cgttgtggat ggcctccgca gaccaggccg
     8341 gcagggcctt cacgaactcg aggtaggcgg agagggcctg gatgtccttc tcggaaaggt
     8401 ccccgatctg gagggggagc ctctccttta ccgagaatct caccgactcg ggggcatatt
     8461 tccggatcca gactctcacc cgctccgcct gtttgaggat gttctcccgg tccgtcacgt
     8521 cgtaactcgt ccgggcgagg cactggaaga gccgccggtc gtcgtgggcg atctgcacca
     8581 ccgtcaccat gtgccggaag gggatgcggg tgggaatgga ggcgatccgg gagagctcgt
     8641 actccctgcc gtctcccttc tcggagacac ggtcgtactc gtcgatgagg gagatgagcc
     8701 ccatgcccgg gtcgaagtcg atggtccgct ccggcctcgt ccgggcgatc aggtagcgga
     8761 gaacatcggg cgggacgatc tcgagcatct cgctgatggt cacggccacg cccttgctcg
     8821 agtgcatcgc ccccttgccc ttcaggctga tccactcgta gatgaccggg tgaggcggct
     8881 cgacccggaa gacccggcgg gcgatctcct tccccgtgtc ataggatccc ccggcggtgg
     8941 catggtcctt cccgaagggc tcgacggtga tcccgaaatg ggcccatcgc atgggccagt
     9001 ccacccgcca gacgagtttt ccctcgccct ttgagtagtc tgacgtgccc tcgttcccgc
     9061 aggcacacct gtaccggacc gtgtggcggg aggggtcgtg ctccaggatc cgggcggggg
     9121 agagcttccc gcagacgctg cagacggggt agaagggcgc ccagtcgggg gggagcgtcc
     9181 gcccggagac ctgctcgagg atgtgccgga tctcgcccgt gtgctcgagg gcgatcccga
     9241 tctccttcgt gtaggccccc gagcggtaga gctcgctccc gagatacacc cggggggaga
     9301 tatccagttc cgagagggcg gagaggaaag gcgtgaggaa gtggtccgcg taactgcggt
     9361 gctccccgca ggggcagggg atgtcggaga tgggtttccc cacgtaggcg gcgtaggact
     9421 cgggaaggaa ggggtaaacc ttccggaggg gatcgaagtc gtcggcgata tagaggagct
     9481 ccacgggcag gccgcgctcc cccatcgcct tgtacacaag gtcgccggtg agaacctccc
     9541 gcatgttccc gatgtggatg ggcccggagg gggtgatccc ggtcgcgatc cggtgctcct
     9601 gtccctccct caccgaggcc gcgaccacgt cggcccaatg gatccgttcg ctcgctcctc
     9661 ccatggtacc aacattcttc gtgttctccc cttatagtgt ttaacacctc tcccgtcatg
     9721 gaccagaaag gacaccggga taccttcacc atggatgacc aggccggcgc tgcccattcg
     9781 ccccggccag ggaaattaca caaggtattt atatacttac ccattctata actatgtaaa
     9841 ggcagccttg tatcagaaaa ccattcgttt tctgggggat caggtcaggg tgcccggggg
     9901 atttgtcggg catgaaaccg ggagagatga gaaaaccggg ccggaaggcc cgcggcatca
     9961 acgaggtcgt gggcttactc ctcatcctcg ccgtcgtcat catgaccatc accgtctata
    10021 tgatctactt catgcccacc ctgggccggg accaggagat ctcccagatg tccgaggtga
    10081 aggagcgctt cgccgagttc aagctgaaca tcgactccct ctggatgacc cgacagggga
    10141 cctccagctt cgggccctcc ctctccctcg gcagcggcga gaccaccggc ctcctcagct
    10201 tcttcccctt cctccgcccg ccgagagccg gcgcggtcct cgcccttaac cagcgggacg
    10261 agaacatcac catcacctcg gactcctata tcctcggggg cgggggatcc aaggtgggcc
    10321 aggccgtcgg tacatctccc gtcctcctct ccgtgaacac cacgccctcg aacctctaca
    10381 tcaagctcta cgcgaccgat ctcctgaagg agaggggcgt gttcgtccac ggcccgacct
    10441 gggacgcctg ggtcaacgtc accccgaact acatctacac cgaccccacc tcctggaccc
    10501 tgaacggcac cgatatcacc gtgaccaccc tcgtcggcgg tagggcggtc gcgacgagcc
    10561 tcccggtcta ccggagcatc agctcctcga ccacctacaa ggtcgacctc atgaaccccg
    10621 tctacggcat ctccccccag ttccagaacc cccagaacct ctggctgtcg gcaagcgacg
    10681 gctccgggat ccttgcctcc tacgagatgc agaactccta cacccccatg gtctctgcct
    10741 cgaccttccc cctcggttcc atcgaattcc ggtccaataa cctctactac accccccaga
    10801 cccactacta ccagctcggc ggcgtcttcc tcgagcagga cgagggttcc accaccgaga
    10861 tcccgcccga catcagcgtc tccctctcta acggcgcgcc cgtggtcaac gtcggggaga
    10921 tcctcctcca gggaaatgcc gtcaccgcca acatgagcgg cagcggcccc atcaccgtca
    10981 cgagccaggt caccggcatc gtgaacgccc ccctcatgcc cggaaaaaat acccggtggg
    11041 tgaacctccg tatccaggcg gcaaacacca atgccgccga catgtggaac cgcaccttcc
    11101 atgaaatcgc cgcccgcgga ggcctccccg ccggctccta tgccgccgga tcctccggta
    11161 ccatcgccta cctgaacatc accggagacc cccagctcta caccatccag ctctccctca
    11221 cccgggtgaa catcgccacc gactacatcc tcgaatactc ccccggcggc atcgcccggg
    11281 gctggcggga ggggcccggc ttccccggcc agccgggaac tgacctgacc ggctgcgggg
    11341 acggcacctg caacggggcc gagacctgca ccacctgccc cggcgactgc gggatatgcc
    11401 ctacctgcgg cgacggcacc tgcaacatcg gggagacctg ctcctcctgc cccggcgact
    11461 gcggagcctg cccccccagc tgcggcgacc tcctctgtaa cggcggcgag acctgctcct
    11521 cctgccccgg cgactgcgga gcctgcccca cctgcggcga cctcatctgc aacggggccg
    11581 agacctgctc ctcctgcccc ggcgactgcg gagcctgcgg cggtggcggt ggcggcggcg
    11641 gcgccggacc cctcgtcatc agcccctgcg actgggtctc ccggaagaag ctcaccatca
    11701 acgggaccat ggtctccggc acccagacca acttcccggt cctcgtcagc ctccccttcg
    11761 actccgacct cgccgccagc gcccaggcga acgggaacga catcgtcttc accgactccg
    11821 gcggctccac cgtcctcccc cacgagatcg agaacttcac gaa
//
LOCUS       DATGDI010000003         7318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_58319, whole genome shotgun sequence.
ACCESSION   DATGDI010000003 DATGDI010000000
VERSION     DATGDI010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58319"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VKO45_00075"
     CDS             <1..228
                     /locus_tag="VKO45_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HMA04308.1"
                     /translation="GFITPGVKALKLYNSKGVAIAPSIATVLDHTYPISRPLYMITQG
                     QPSGLARDFIDFILSPDGQKIVEEQGFVPLS"
     gene            374..2299
                     /gene="pstA"
                     /locus_tag="VKO45_00080"
     CDS             374..2299
                     /gene="pstA"
                     /locus_tag="VKO45_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HMA04309.1"
                     /translation="MRSERVIEALLFLSSTAAILSVLFILLYLFLQAAPIFPLAGVGD
                     FIAGTSWDPTGNPPQYGTLPLTVGTLLVTLGAMIISIPLSLGCAIFTAEIASTRVRAV
                     LKPAIELLAGIPSVVYGFFGLVVLTEVLRVGFDLSSGLTWLAGSILLAIMALPTITSV
                     SEDAIRAVPREYREGSLAIGATRWQTMSRVIIPAAFSGISAGVILGMGRALGETMAVL
                     MVTGNAAVIPSPIWNVFSPIRTLTGTLGIEMGEVASGSAHYQALFGVAILLLVITLLV
                     NLTASAVIGRIRSGAVAVVPCGVDGRTPLRYRISRGARNILAKARVPAIAAAGILLLA
                     LITLSLGLTAALALAAAAGAFLLLRGRLSRRMTERIAFFFVTAATVIVLLVLGVILWN
                     IIGNGLPAISWEFLTGVPRDLGRSGGIFPAIVGTLYLVGGAIGMALPVGVGAAVYLVE
                     YTREGRFVRLVRTGVDLLNGTPSIVFGLFGFAFLVLYLRFGVSLIAGQVTLALMVLPT
                     IIRTTEEALKCVPQGLREGSLALGATRWQTIRRVVLPPAMPGILTGAILAVGRAAGET
                     APIMFTAVVFSRRFLPDSLLDPVMALPYHLFILATNVPGARTNQYGTALVLLALVIGI
                     YAIAILLRDRFQRGVSW"
     gene            2302..3066
                     /gene="pstB"
                     /locus_tag="VKO45_00085"
     CDS             2302..3066
                     /gene="pstB"
                     /locus_tag="VKO45_00085"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HMA04310.1"
                     /translation="MSGSQTIIATENLNLWYGEKQALEGITMAVERNRVTALIGPSGC
                     GKSTLIRVFNRMNDLIGSCRVEGRVLIDGEEVYGPAADVVAIRKKVGMVFQKPNPFPK
                     SVYENVAYGPRVHGVTDSGDLDRIVKESLQSAALWNEVKDRLHDPATGLSGGQQQRLC
                     IARCLAVEPEIILMDEPCSALDPIATAKIENLIDHLKRDYTVVIVTHNMQQAARVSDS
                     TGFMYLGKLIEFGETTQIFEKPREELTANYVTGRFG"
     gene            3075..3725
                     /gene="phoU"
                     /locus_tag="VKO45_00090"
     CDS             3075..3725
                     /gene="phoU"
                     /locus_tag="VKO45_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HMA04311.1"
                     /translation="MVEKFHAELEMMKEHALEMGALSRAMLQMSIRALGNEDLELAAW
                     VMKKRESLRTWDESVEEEALRLMALYQPMAKDLRTIAATLKMITYLTRIGIYGKDVAV
                     VVDRSAGMQPIPGLFHIPRMTEIVCGMVDAGLDAYAKESTSPIGDIGKRDDEVDELFL
                     STLNEGIAAMTADPARIPICTNYILVARYLERCGDHACKIAEKVHYMVTGERIDIQ"
     gene            3984..4658
                     /locus_tag="VKO45_00095"
     CDS             3984..4658
                     /locus_tag="VKO45_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04312.1"
                     /translation="MSRGSPARPRPGRGTGRLLRRDVIHEKVRSGVTYVVSEDYSYKT
                     EAYYRILTLEMEGKQPVPSTRDLLDAYVVPVCLARAGKAGIPVCEWGISDERVPLPSI
                     LYGISYFADPAEYSIVRDLETAGPVIKYITGNGKYPFCYEPIPESAEIVPCVAIFGRT
                     ADAPGPLADLAAKVYRLFRIPLMEVVSVENGGYRLSAITPVWYSTLSRDEKRILEGIL
                     TGECDG"
     gene            4651..5550
                     /locus_tag="VKO45_00100"
     CDS             4651..5550
                     /locus_tag="VKO45_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HMA04313.1"
                     /translation="MARLGLFVDRQTLGNSEQLTALIRLRDSAEEMGHTVYFIFPTEI
                     AKIAKVDGLFIRSRTDPLNISFVAAKMAELRGIPVIDDPESIRVCSDKVNMYLHLMHH
                     GVRIPETVFVGKRDLTEKTAGDLLRNLGTPLILKEPSTSFSLRVEKVHTVPEFLKTAK
                     RFIKLSDWIVAQRFIESDFDWRVGVLDGEPLYACQYIIPQETFKIQATVNGHLVYCAV
                     KSLPLVQVPAPVLDIAVSAAKAIGRGFYGVDIKDHEGTPFVIEVNDNPSLEGGEDEHS
                     PGLYRKIIGHLIGGGDAAGTASP"
     gene            5560..6789
                     /locus_tag="VKO45_00105"
     CDS             5560..6789
                     /locus_tag="VKO45_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HMA04314.1"
                     /translation="MAFAPSIPVIVLILVFLGIAIRKVGRFSLRIWQIMLIGAVAVLV
                     TGQISPLSAARAVNPDVMIFLFGMFVVGVALRESGYLLHLSNRICRRAENRNQLLLLL
                     VFSFGILSAILMNDTLAIIGTPLVLSFARSSRISPKLLLLTLAFSITTGSVLSPIGNP
                     QNLLIAINGPSGNPFITFALYLAIPTVVGLGLVYLFLRVAFRGEPWKEPLSLPPGYVK
                     DERLAFITRVSLVVLITFIAVKVASFFVGLDFQVPLPAIALLSALPILAFSERRVEIA
                     RKVDWETLVFFAAMFVLMESVWESGFIQSLVPIFGAGIPVILGLSIAASQFVSNVPWV
                     ALYLPALPSTSPAALMALAAGSTIAGNLSILGAASNVIIIQNAEKEGETLTFPEFVKI
                     GLPLTLVQTLVYLLFLG"
     gene            complement(6790..>7318)
                     /locus_tag="VKO45_00110"
     CDS             complement(6790..>7318)
                     /locus_tag="VKO45_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMA04315.1"
                     /translation="ADIYSLPFADGSFDHVFVCFVLEHLPDPGLALTLLRRVLAPGGT
                     ITVIEGDHGSAFFHPDSRAAGRTIACLIALQREKGGNPLIGREVYPLLSGAGYREVFV
                     SPRMVYVDATHPDLVEGFTKNTFTAMVEGVREEVLERTMIGAEEWEQGIRDLYRTCEP
                     DGTFCYTFFKGTGVK"
BASE COUNT         1239 a         2571 c         2153 g         1355 t
ORIGIN      
        1 gggtttatca cccccggcgt caaggccctg aaactctaca actcgaaagg cgtggcgatc
       61 gccccgagca tcgcgaccgt ccttgaccac acgtatccca tctcccgccc cctgtacatg
      121 atcacgcagg gccagccgtc agggctcgcg agggacttta tcgatttcat cctctcgcct
      181 gacgggcaga agattgtcga ggagcagggc ttcgtgcccc tctcctgatc accccgggag
      241 gtgaagtccg gaaatgaacg ccccgatgcg ggatcgaccc ccgaccccac tcctcccccg
      301 cgatctcctc cggccaacat tcctgttcca ggctctccgc ctccagggtt cccccgaaaa
      361 ggccggaagg cggttgagga gcgagcgggt tatcgaggcc ctcctcttcc tctcctcgac
      421 cgcggcaatc ctctcggtgc tcttcatcct cctctatctt ttcctccagg ccgcgcccat
      481 ctttccctta gccggcgtcg gagacttcat tgcgggtact tcctgggacc cgaccgggaa
      541 cccgccgcag tacggaaccc tacccctcac ggtgggaact ctcctcgtca ccctcggggc
      601 gatgattatt tccattcccc tctccctggg gtgcgccatc ttcacggccg agatcgcctc
      661 cacccgggtc agggcggtct taaagccggc catcgagctc ctggcgggga tcccgtccgt
      721 cgtgtacggg ttctttggcc tcgtcgtgct gaccgaggtc ctccgggtcg gcttcgacct
      781 ctcctcaggc ctcacatggc ttgcgggttc catcctcctt gccatcatgg ccctccccac
      841 catcacctcc gtctcggagg acgccatccg ggcggtcccc cgggagtacc gggagggaag
      901 ccttgccatc ggggccaccc ggtggcagac catgagccgg gttatcatcc cggcggcctt
      961 ctcggggatc tccgccgggg tcatcctggg catggggagg gccctcggag agaccatggc
     1021 ggtgctcatg gtcaccggga acgcggccgt catcccgagc cctatctgga acgtgttctc
     1081 ccccatccgg accctgaccg ggaccctggg gatcgagatg ggggaggtcg cctccgggag
     1141 cgcccactac caggccctct tcggcgtcgc catcctcctc ctcgtcatca ccctcctcgt
     1201 gaacctgacc gcttcggcgg tcatcgggag gatccggagc ggagcggtgg cggtggtacc
     1261 ctgcggggtc gacgggagga cacccctccg gtacaggatt tcccgggggg cccggaacat
     1321 cctcgcaaag gcccgggttc ccgccatcgc cgccgcgggg atcctcctcc tcgccctgat
     1381 caccctgtcc ctcggcctca ccgcagccct ggccctcgcc gcggcggccg gggccttcct
     1441 tctcctccgc ggacggctct cccggcggat gacagagagg atcgccttct tcttcgtcac
     1501 cgccgctacc gtcattgtac tccttgtcct cggggttatc ctctggaaca tcatagggaa
     1561 cggcctcccg gccatctcct gggagttcct gaccggggtg ccccgggacc tggggaggag
     1621 cggcgggatc ttcccggcca tcgtcggcac cctgtacctg gtgggtgggg ccatcgggat
     1681 ggcgcttccc gtcggcgtgg gcgccgcggt ctacctggtc gagtacaccc gcgagggccg
     1741 gttcgtgagg ctcgtgagga ccggggtcga cctcctgaac ggaaccccct ccatcgtctt
     1801 cggcctcttc ggcttcgcct tcctcgtcct gtacctccgg ttcggggtct ccctcatcgc
     1861 ggggcaggtc accctcgccc tcatggtcct cccgaccatc atccggacca ccgaggaggc
     1921 cctgaagtgc gtcccgcagg ggctccggga ggggagcctt gccctcgggg caacccggtg
     1981 gcagacgatc cgccgggtcg tgctcccgcc ggcgatgccc gggatcctca ccggcgccat
     2041 tctcgccgtg ggacgggccg cgggggagac cgcccccatc atgttcaccg ccgtcgtctt
     2101 ctcccggcgg tttctccccg attccctcct cgatcccgtc atggccctcc cctaccacct
     2161 cttcatcctt gcgacgaacg ttcccggtgc gaggaccaac cagtacggca cggccctcgt
     2221 cctcctcgcc ctcgtcatcg ggatctacgc catcgccatc ctcctcaggg accgcttcca
     2281 gaggggagtg agctggtagc tatgtcagga agccagacta tcatcgcgac cgagaacctg
     2341 aacctgtggt acggggagaa gcaggccctc gagggcatta ccatggcggt ggagaggaac
     2401 cgggtgacgg cccttatcgg cccctccggc tgcgggaagt ccaccctcat ccgggtcttc
     2461 aaccgcatga acgacctcat cggctcctgc agggtcgagg gcagggtcct catcgacggg
     2521 gaggaggtat acggtcccgc cgccgacgtg gtcgccatcc ggaagaaggt cgggatggtc
     2581 ttccagaagc cgaacccctt cccgaagtcg gtgtacgaga acgtcgccta cggcccgagg
     2641 gtccacgggg tcacggacag cggggacctc gaccggatcg tgaaggagag cctccagtcg
     2701 gcggccctct ggaacgaggt gaaggaccgg ctccacgacc ccgccaccgg cctctcggga
     2761 ggccagcagc agcggctctg catcgcccgg tgcctcgccg tcgagccgga gatcatcctc
     2821 atggacgagc cctgctcggc cctcgacccc attgcgacgg cgaagatcga gaacctcatc
     2881 gatcacctca agagggacta taccgtggtc atcgtcactc ataacatgca acaggcggcc
     2941 cgggtctcgg attccaccgg gttcatgtac ctgggaaagc tcattgagtt cggggagaca
     3001 acacagatct tcgagaagcc ccgtgaggag ctcacggcca actatgtgac gggccggttc
     3061 ggataggtgg cagtatggta gaaaagtttc acgcagaact cgagatgatg aaggagcatg
     3121 ccctcgagat gggcgccctc tcccgggcca tgctccagat gtccatccgg gccctgggga
     3181 acgaggacct tgagctcgcg gcctgggtga tgaaaaagag agagagcctg aggacatggg
     3241 acgaatccgt cgaggaagag gccctcaggc ttatggccct ctaccagccc atggcaaagg
     3301 acctccggac catcgcggcc accctgaaga tgatcaccta ccttaccagg atcgggatct
     3361 atggaaagga cgtcgcggtg gtggtggaca ggagcgcggg gatgcagccg atccccggcc
     3421 tcttccacat cccccgcatg acggagatcg tctgcgggat ggtcgatgca ggcctcgatg
     3481 cctatgcaaa ggagagcacc tcgcccatcg gggatatcgg aaaaagggac gacgaggtgg
     3541 acgaactctt cctctcaacc ctcaacgagg gcatcgccgc catgaccgcg gatccggcga
     3601 ggatccccat ctgcaccaac tacatcctcg tcgcccggta cctggagcgc tgcggggacc
     3661 atgcctgtaa gatcgcagaa aaggtccact acatggtcac cggcgagagg atagatatcc
     3721 agtaaccacc cctctcctcc ttcgaaccct gtcgaacgac ccgaaccccg gcgatggatc
     3781 cgattacccc tctcctgctc gataccctga aataacggga tgggcatact acatcatgga
     3841 caccgggagg gtgtaactcg tatttcagct cttcttccat ccctgcccat cacacggggg
     3901 cgatcctcct cagggcatcc ccggacagga gaaggatcca gagacagccc cggggaatcc
     3961 cgcatcgggg tgagccaccg ggcatgagcc gcggatctcc ggcacgccca cggccgggta
     4021 gggggacggg cagactcctc cggcgcgacg tcatccacga gaaggtacga agcggcgtta
     4081 cctacgtcgt gagcgaggac tactcgtaca agaccgaggc ctactaccgg atcctcaccc
     4141 tggagatgga ggggaaacag cccgtcccct cgacgaggga tctcctcgat gcctacgtgg
     4201 tcccggtctg cctcgcccgg gcaggaaagg ccgggatacc ggtctgcgaa tggggaatct
     4261 ccgacgaacg tgttcccctc ccctccatcc tctacgggat atcctacttt gccgaccccg
     4321 cggagtacag catcgtccgg gacctcgaga ccgccgggcc ggtcatcaag tacatcaccg
     4381 ggaacgggaa gtaccccttc tgctacgagc ctatccccga gtccgcggag atcgtcccct
     4441 gcgtcgccat cttcggccga acggcggatg cccccggacc cctcgccgat ctcgccgcga
     4501 aagtctaccg ccttttccgg atacccctga tggaggtcgt ttcggtggag aacgggggat
     4561 accggctctc ggccatcacc ccggtctggt attccaccct ctcccgggac gaaaaaagga
     4621 tcctcgaggg gatcctcacg ggtgagtgcg atggctaggc tcgggctctt cgtggaccgg
     4681 cagaccctgg ggaactccga gcagctcacg gccctgatcc ggctccggga ctccgccgag
     4741 gagatggggc acactgtcta cttcattttc ccgaccgaga tagcgaagat cgccaaggtc
     4801 gatggtctct tcatccggag caggacggac ccgctgaaca tcagtttcgt ggccgcgaag
     4861 atggccgagc tccggggtat cccggtcatc gatgacccgg agtccatccg ggtctgttct
     4921 gacaaggtga acatgtacct tcacctcatg caccatggtg tccggatccc cgagacggtg
     4981 tttgtgggaa agagggatct caccgagaag accgcgggag acctcctccg caacctcggt
     5041 acgcccctca tcctcaagga gccgtccacc tctttctccc tccgggtgga aaaggtccac
     5101 accgtacccg agttcctgaa gacggcgaag aggttcatca agctctcgga ctggatcgtc
     5161 gcccagaggt tcatcgagag cgacttcgac tggcgggtgg gggtgctcga cggggaaccc
     5221 ctctatgcct gccagtacat catcccccag gagaccttca agatccaggc cacggtgaac
     5281 gggcacctcg tctactgcgc ggtgaagagc ctgcccctcg ttcaggtccc cgccccggtc
     5341 ctcgatatag ccgtttcggc ggcaaaggcc atcgggaggg ggttctacgg ggtcgacatc
     5401 aaggaccatg aaggcactcc cttcgtcatc gaggtgaacg acaacccctc cctcgaggga
     5461 ggcgaggacg agcactcccc cgggctctac cggaagatca tcgggcacct catcggcggc
     5521 ggggacgccg cgggaaccgc cagcccctga cgggatccca tggcctttgc accgagcatc
     5581 ccggtcatcg tcctcatcct ggtcttcctc ggcatcgcca tcaggaaggt cggccggttc
     5641 tccctcagga tctggcagat catgctcatc ggcgcggtgg cggtcctcgt caccggccag
     5701 atctcgcccc tctcggccgc gagagccgtc aacccggatg tcatgatctt cctcttcggc
     5761 atgttcgtgg tcggggtcgc cctccgggag agcgggtacc tcctccacct ctcgaaccgg
     5821 atctgccgga gggcggaaaa ccggaaccag ctcctcctcc tcctcgtctt ttccttcggg
     5881 atcctctcgg ccatcctcat gaacgacacc ctcgcgatca tcgggacccc cctcgtcctc
     5941 tcctttgccc gatcgagccg gatctccccg aaactcctcc tcctcaccct tgccttctcc
     6001 atcaccacgg ggagcgtcct ctcccccatc gggaaccccc agaacctcct cattgccatc
     6061 aacggcccgt ccgggaaccc cttcatcacc tttgccctgt acctcgctat ccccacggtg
     6121 gtgggcctcg gcctcgtcta cctctttctc cgcgtcgcgt tccgtggcga gccctggaag
     6181 gaaccgctca gccttccccc ggggtacgtg aaggacgagc ggctcgcctt cattacccgg
     6241 gtctccctcg tggtcctcat caccttcatc gccgtgaagg tcgcctcttt ctttgtcggc
     6301 cttgatttcc aggttcccct cccggccatc gccctcctct cggccctccc catcctcgcc
     6361 ttctcggaaa ggagggtgga gatcgcgcga aaggtcgact gggagaccct cgtcttcttc
     6421 gccgccatgt tcgttctcat ggagagcgtc tgggagtccg ggttcatcca gtccctcgtc
     6481 ccgatcttcg gcgccgggat ccccgtgatc ctcgggctct ccatcgctgc ctcccagttc
     6541 gtctcgaacg ttccctgggt cgccctgtac ctgcccgccc tcccttcgac ctccccggcc
     6601 gccctcatgg ccctcgccgc ggggagcacg attgcaggga acctctccat cctcggggcg
     6661 gcctccaatg tcatcatcat ccagaacgcc gagaaggagg gggaaaccct caccttcccc
     6721 gagttcgtga agatcgggct ccccctcacc ctggtccaga ccctcgtcta cctgctcttc
     6781 ctcgggtgac tattttaccc cggtcccctt gaagaaggtg tagcagaagg tcccgtcggg
     6841 ctcgcaggtc cggtagaggt cccggatccc ctgttcccat tcctctgccc cgatcatggt
     6901 ccgctcaagg acctcttccc tgaccccctc gaccatcgcg gtgaaggtgt tcttcgtgaa
     6961 tccttcgacg agatctggat gggtggcatc cacgtacacc atgcggggag agacgaaaac
     7021 ctcacggtac cccgcccccg agaggagcgg gtagacctcc cgcccgatga gggggttccc
     7081 gcccttctcc cgctggaggg cgatgaggca ggcgatggtc ctccccgccg cccggctgtc
     7141 cggatggaag aaggcagaac catgatcacc ctcgatgacc gtgatggtac ctccgggggc
     7201 gaggacccgt ctgaggaggg tgagggcaag accggggtca gggaggtgct cgaggacgaa
     7261 gcagacgaag acatggtcga agctgccgtc cgcgaacggg agggagtaga tgtccgcc
//
LOCUS       DATGDI010000004         8615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_291052, whole genome shotgun sequence.
ACCESSION   DATGDI010000004 DATGDI010000000
VERSION     DATGDI010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8615
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291052"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..>8615
                     /locus_tag="VKO45_00115"
     CDS             108..>8615
                     /locus_tag="VKO45_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMA04316.1"
                     /translation="MEAPRERKIGGKTAEEWRDLGYHEKDPEKRVRYYSFSLQIDPRN
                     LRAWYQQGLALHRLGRLTEAAEALGKALELDPAHPDARFSLGQVQRDQKEYGKAVGTF
                     DEILGREPGHAGALNAKGHALLELKRPDAALETFDRLLAVDAGRADAWYARGVALIDL
                     DRPSDAIESLDRALGIDPGHAAAWSTKGIALVGLGKSDEALGALDHALELAPRHTGAW
                     HAKGLVLLGLKRPDEALGAFDHVIEINPRDVAALYSRGIALMEMRRFPQALETLDRAL
                     GIDPAHAGAWFLKGQALIDLERFGDALGALDRALEIDPGQAGVWYSRGLALEELGRFA
                     EAVKALDRALEVDPEHARAWYTRGLALAEIDRNEEAVASLSRVLAVKAEDPNTLGIKG
                     LALKAMGRYGEAVETLDRSLDGEPENANGWYNRGLCLRELGRDREAVESFDRTLAIEK
                     DLGVIWYNRGLALTELGDLPAAVESFDRSLALEGDHFGSWYRRGVALAGLGRFQESVE
                     SLDHAVALEPDLSVAWFHRGLAFAGMGKNRESLRDLDHTLSLDPSHAAAWYQKGLLLH
                     RQGRREEGLACLEKATRFDTANRDAWYEKGRLLLELERFSDGEKALGRALDLDPANGE
                     AWFLRGEALLRLDRAAEAIPALTESTRIEPDRAEAWFDLGSALLGLGRHEEAAGVLGR
                     AAGLDPLNATLHLRLGIALMGLGRNEEAEKAFGEAAGLDPTLAEAWSRRGQVLAGLGR
                     YEDAATTLSRSLDLNREDGPAWFSLARVQERLGRRELAVQSLERGLSLAPSHEDAWYR
                     KGLLLYELQRFEEAKQSLDEALRLAPDRAEAWFRHGIILLALQKPEEAAASLRRGLEI
                     DPSAAEGWSQLARAQEEAGNVAGAVEALRSSTERLPGDSAAWLRLGELQLHIGKPEEA
                     LSSGEKAVEIDSSLAAGWFLRGRALSALRRPEEAHESLGRSLSLDPGQGEAWFRDGLA
                     LEELGRHRDAVASFDRAITVSPKSIGAWYGKGRSLQALGRSRDSLQALEKGLTIEPAS
                     PEDWFSRGLILEDLGRYQDEVPSLEKALRANPDDAQAWFHRGVALSALGRTEEAAEAF
                     RETLRIDPEDARASFQLGSLERDLGHYDQAVEALEKATRMSPADARAQYLAGLARNGQ
                     GNHEAAALAFRKSLEIEPSDGRVWYTLGWTLVKLGRFDEGREAFERATALDPTNFQAW
                     YNRAFCEKELGLHHQAIASIEKALKIDAGHDGAWFLKGFSQKELGRFEDAAASFGRAT
                     TLNEANGVGWFMLGWTLAKLGTYDRAVQAFDAAIRIHPDDAGAFYNKALALIALGKPR
                     EALEALNASLERDPSYAPAWFQLGSTLASQGLHAKAVEAFGTGLGLEPGHAPIWFQEG
                     LSLLALEREEQAVTAFTRALEADPGDTESRCHLGYALAAVGRMEEAIHAFDRVLEADP
                     SRHLAWYHRGIAEMELGRHEEALVSFDHALEIDREDARSWYQKGLALAALSRHGDAIK
                     AFGNALIISPDDAGSWYYSGLSLIEEGRTGEALSALDRSLALDPGDLHARFHRGVCLS
                     RLGRTEEAVKAFEEVTRIDPLHSRAWFLKGLALRDLGQPGKAISSLVRALEIDPSCAP
                     CTYEKGLILEAENRAAEALPAFDRALELSPSDAEAHFHRGLALVSLNREAEGLMAFES
                     ATGIAPGHAGAWIQKGRVLARTGRSAEAAEAFDTATRINPGDPGAWFDKGLAYASIGG
                     HREAVAALERALTLEPEKAEAWFRKALSLSALGDPSGAIVALDRAIALNPTDGETWHR
                     KGLALQALGKPDEAREALTHSVELEPGNPEAWFHLGEAGRDLGREEEAASALEVAVGL
                     NPEYRDAWFLMGLVREVTGELEGALTAFTRCVELDGRHADGHYHRGLTLVALGRPEDA
                     VEAFDQALDIDPSRPDAWFQEGLVLEQFGEYNRAVSALETSLSLDPNQAEAWLHIARS
                     RSAQGRPADAVAACGQVIRIDPRHSPALTLKGLSEEQLGEGEQALQSFATSLGIDPEQ
                     PEAWHHRGSVLLSLRRFAEAVDSLDRSLTLAPRNGKALYEKGIAYIGTGATEEAVTAL
                     GASLDLEPENADGWYRRGTALASLGRQADAIGDLDRSLALDPGNPLVWLEKGLALQAM
                     GNLPGAMEAFRASIDADPSLSRPWLLLGVAARLSGRMGDALTALDRATRADPEDPEGW
                     HERGLVLMELGRTEEGISSFDRALGIRPDHAGSLYSRALALEILGRHEEALGSMERAL
                     PSKESDPAAWFHHGSLLFRLSRDEEAARSFDRSLALDPGQISAWFQRGETLERLGRFE
                     TALESFDRAVRLDPGLTGGFYHMGICRERLGDHAGAAEAFFRVLESEPENPGALYHRG
                     LALMGTRNYPEAAGSLEKAIALSPSADAWYQLGVARERLGRFSDAVEALDQALATDPD
                     RAPAWCLRGVALAKGGDHAGAIGSFEKSLALEPGNGRAWFGRGYSLQVLGRLPEAKEA
                     IEKAMDLSYTGAPVLLRYGLVLEDLGDHDGASRSFTRVVELDPGSGDGFLHLGLAEFA
                     LGKAGEAALAFEKAAKIQGRPSPAWFHLGRAYLSLGKDEDALGPLGEAERLEPDNAEA
                     WFLEGLALHRLGRFKKAVISLEKAVRLNPSHARAWYQLGLARATLDDQGEAARAFGRA
                     TDLDPSSGPAWYGLGAASRRIGENQRALDALDHAVSLDPAAPAGLMERGLTLGNLGRY
                     AEALSAFDRVLEIAPGDKPALIARVETLASLERFGEGLAAAQRMLDRDPSLAAAWYLR
                     GRCLSGLGRHGEAVESLGRALELDPDQASAWHQLGLVQEARGFFGDAADAFWRAGQIA
                     PEEGSSWL"
BASE COUNT         1274 a         3073 c         3028 g         1240 t
ORIGIN      
        1 gagggggtgc cagtaaagat ataaaccaat tttccagata tataaagaga gcaatttttt
       61 gccggccggt gcgatgagag ggaacccgat gtcgccgggg tgagggaatg gaggcgccgc
      121 gtgagaggaa gatcggcggg aagaccgccg aggaatggcg ggacctgggg taccatgaga
      181 aggaccccga gaagcgggtc cggtactatt ccttctctct ccagatagac ccccggaacc
      241 tccgtgcctg gtaccagcag ggcctcgccc tccaccggct gggcaggctc accgaggcgg
      301 cagaggccct cggaaaggcc ctcgagctgg accctgccca cccggatgcc cggttctccc
      361 tcgggcaggt ccagagggac cagaaggagt acgggaaggc ggtcgggacc ttcgacgaga
      421 tcctcgggag ggagcccggc catgccggcg ccctgaacgc gaagggccat gccctcctcg
      481 agctgaagcg gcccgacgcc gccctcgaga ccttcgaccg gctcctggcc gtcgacgccg
      541 gccgcgccga tgcctggtac gcccgggggg tcgccctcat cgacctggac cgtccctccg
      601 atgccatcga gtccctggac cgggccctcg ggatcgaccc ggggcatgcc gccgcctggt
      661 ctacgaaggg catcgccctc gtggggctgg gaaagtccga cgaggccctc ggggccctgg
      721 accacgccct ggagctcgca ccccggcaca ccggcgcctg gcatgcgaag ggtctcgtcc
      781 tcctcgggct gaaacggccc gacgaggccc tcggcgcctt cgaccatgtc atcgagatca
      841 acccccggga cgtggcggcc ctctactccc ggggcatcgc cctcatggag atgagacgct
      901 ttccccaggc cctggagacc ctggaccggg ccctcgggat cgaccccgcc catgccgggg
      961 cctggttcct gaaggggcag gccctcatcg acctggagcg cttcggggac gccctcgggg
     1021 ccctggaccg ggccctcgag atcgacccgg gccaggccgg tgtctggtac tcgagagggc
     1081 ttgccctcga ggaactcggc cggttcgccg aggcggtgaa ggccctggac cgggccctcg
     1141 aggtcgaccc ggagcatgcc cgggcctggt acacccgggg gctcgccctt gcggagatcg
     1201 accggaacga ggaggcggtg gcctcccttt cccgggtcct cgcggtgaag gccgaagacc
     1261 cgaataccct gggcatcaag ggcctcgccc tcaaggccat gggccggtac ggggaggcgg
     1321 tggagacgct cgaccggtcc ctggatgggg agccggagaa cgcgaacggc tggtacaacc
     1381 ggggcctctg cctgcgagag ctcggccggg accgggaggc ggtggagtcc tttgaccgga
     1441 ccctcgccat cgagaaggac ctgggggtga tctggtacaa ccgcggcctc gccctcacgg
     1501 agctcgggga tctcccggcc gcggtcgagt ccttcgaccg gagcctcgcc ctcgaagggg
     1561 atcacttcgg gtcatggtac cggcgggggg tagcccttgc cggcctgggc cggttccagg
     1621 agtcggtgga gtccctcgac catgccgtcg ccctcgagcc cgatctttcc gtcgcctggt
     1681 tccaccgggg cctcgccttc gccgggatgg gaaagaaccg ggagtccctc cgggacctgg
     1741 atcacaccct ctccctcgac ccgagccatg ccgcggcctg gtaccagaag gggctcctcc
     1801 tccaccgtca ggggaggagg gaggagggcc tcgcctgcct ggagaaggcg acccggttcg
     1861 acaccgcgaa ccgggatgcc tggtacgaga aggggcgcct cctcctggag ctcgagcggt
     1921 tcagcgacgg tgagaaggcc ctcgggcgcg ccctggatct cgacccggcc aacggggagg
     1981 catggttcct ccggggggag gccctgctca ggctggatcg agccgcggag gcaatccccg
     2041 ccctcacgga gagcacccgg atcgaaccgg atcgggccga ggcctggttc gacctgggaa
     2101 gtgcgctcct cggcctcggc aggcacgaag aggcggcagg ggtcctcggg cgggcggccg
     2161 gactcgaccc cctgaacgca accctgcacc tgcggctcgg tatcgccctc atggggctgg
     2221 gccggaacga ggaggccgag aaggccttcg gcgaggcggc aggcctggac cccaccctcg
     2281 cggaggcctg gtcccggaga gggcaggtcc tcgccggcct cgggaggtac gaggacgcgg
     2341 ccaccaccct ctcccgttcc cttgacctga atcgggagga cggtcctgcc tggttctccc
     2401 ttgctagggt ccaggaacgg ctcgggaggc gggagctcgc ggtccagtcc ctcgaacggg
     2461 ggctctccct cgccccctcc catgaggatg cctggtaccg gaaggggctc ctcctgtacg
     2521 agctgcaacg gttcgaggag gcgaaacagt ccctcgacga ggccctccgc ctcgcgccgg
     2581 accgggccga ggcctggttc cgccacggga tcatcctcct cgccctccag aagcccgagg
     2641 aggcggcggc ctccctccgg agggggctcg agatcgatcc ctctgcggcg gaggggtggt
     2701 cccagctggc ccgggcccag gaggaggcgg ggaatgtcgc cggggccgtg gaggccctca
     2761 ggagctccac ggaacggctg ccgggggatt ccgcggcctg gctccggctc ggggaactgc
     2821 agctccacat cgggaaaccg gaggaggcac tctcctcggg agagaaggcc gtggagatcg
     2881 actcctccct cgccgcgggc tggttcctcc gggggagggc cctctccgcc ctccgccggc
     2941 cggaggaggc ccacgaatcc ctcgggcgct ccctctccct cgaccccggc cagggcgagg
     3001 catggttccg ggacggcctt gccctcgagg agctcggccg gcaccgggat gccgtggcct
     3061 cctttgaccg ggccatcacg gtaagcccga agtccatcgg cgcatggtac gggaagggcc
     3121 ggtccctcca ggccctgggc cggagccggg attccctcca ggccctcgag aagggcctta
     3181 ccatcgagcc cgcgagcccc gaggactggt tctcccgggg gctgatcctc gaggacctgg
     3241 ggaggtacca ggatgaggtc ccctccctcg agaaggccct gcgggcgaac ccggacgatg
     3301 cgcaggcctg gttccaccgc ggggtcgccc tctcggccct gggaaggacc gaggaggccg
     3361 ccgaggcttt ccgggagacg ctcaggatcg acccggagga tgcgagggcg agcttccagc
     3421 tggggtccct ggaaagggac ctcggccact acgaccaggc ggtggaggcc ctcgagaagg
     3481 cgacccggat gtccccggcg gacgcgcggg cgcaatacct cgccggcctc gcccggaacg
     3541 ggcaggggaa tcacgaggcg gctgccctcg ccttccgcaa gtccctcgag atcgagccct
     3601 ccgacggccg ggtctggtac accctcgggt ggaccctcgt caagctgggc aggttcgacg
     3661 agggccggga ggccttcgag cgggccacgg ccctggaccc gacgaacttc caggcctggt
     3721 acaaccgcgc cttctgcgag aaggagctcg ggctccacca ccaggccatc gcctccatag
     3781 aaaaggccct caagatcgat gcggggcatg acggggcctg gttcctgaag gggttctccc
     3841 agaaggaact cggcaggttc gaggacgccg cggcctcctt cggccgtgcg accaccctca
     3901 acgaggcaaa cggggtcggg tggttcatgc tcggctggac cctcgcgaag ctcgggacct
     3961 acgaccgggc cgtccaggcc ttcgatgccg ccatccgcat ccacccggac gacgccggcg
     4021 ccttctacaa caaggccctc gccctcatcg ccctcgggaa gccgcgggag gccctggagg
     4081 ccctcaacgc ctccctggag agggacccct cgtacgcacc ggcctggttc cagctcggct
     4141 ccaccctcgc ctcccagggg ctccacgcga aggccgtgga ggcctttggc acgggcctcg
     4201 gcctcgagcc cggccatgcc cccatctggt tccaggaagg gctatccctc ctcgccctcg
     4261 agagggagga acaggcggtc accgccttta cccgggccct cgaggcagac cccggcgaca
     4321 ccgagagccg gtgccacctc gggtatgccc tcgccgccgt cggcagaatg gaggaggcga
     4381 tccatgcctt cgaccgggtg ctcgaggccg atccctcccg gcacctggca tggtaccacc
     4441 ggggcatcgc cgagatggag ctcgggaggc atgaggaggc cctcgtctcc tttgaccatg
     4501 ccctcgagat agaccgggag gacgcgcggt catggtacca gaaggggctc gccctcgccg
     4561 cgctctcacg gcacggggat gccataaagg ccttcgggaa tgccctcatc atctcccccg
     4621 atgacgcggg ctcgtggtac tactcggggc tctccctcat cgaggagggc cggaccgggg
     4681 aggccctctc cgccctcgac cggtccctcg ccctcgatcc gggggacctc cacgcgaggt
     4741 tccaccgcgg ggtctgcctc tcccgcctgg gaaggaccga ggaggcggtc aaggccttcg
     4801 aggaggtcac ccggatcgat cccctccatt cccgggcctg gttcctgaag gggcttgccc
     4861 tccgggatct cgggcagccc gggaaggcca tctcgtccct ggtacgggcc cttgagatcg
     4921 acccctcgtg cgccccctgc acctatgaaa agggcctcat cctcgaagcc gagaaccggg
     4981 ccgccgaggc cctccccgcc ttcgaccggg ccctcgagct ctcgccatcc gacgccgagg
     5041 cccattttca ccgtggcctc gccctcgtct ccctcaaccg ggaggccgag ggtcttatgg
     5101 ccttcgagag tgccacaggc atcgcccccg ggcatgccgg ggcctggatc cagaaggggc
     5161 gggtcctcgc ccgcaccggc cggtcggcgg aggccgccga ggcctttgac acggcgacga
     5221 ggatcaatcc cggggacccg ggggcctggt tcgacaaggg cctcgcctat gcctccatcg
     5281 gcggacaccg cgaggccgtc gccgccctgg aacgggccct cacgctcgag ccggagaagg
     5341 cggaggcctg gttccggaag gccctctccc tctctgccct cggggaccct tcgggggcca
     5401 tcgtggccct ggaccgggcc atcgccctca accctaccga cggggagacc tggcaccgga
     5461 agggacttgc cctgcaggcc ctcgggaagc ccgacgaggc gcgggaggcc ctcacccatt
     5521 ccgtcgagct ggagcccggg aacccggagg cctggttcca cctgggggag gccggaaggg
     5581 acctcgggag agaggaggag gcggcatcgg ccctcgaggt ggccgtaggg ctgaacccgg
     5641 agtaccggga tgcctggttc ctcatgggac tcgtccggga ggtcaccgga gagctcgagg
     5701 gcgctctcac cgccttcacc cggtgcgtgg agctggacgg ccggcacgcc gacgggcatt
     5761 accaccgggg cctcaccctc gtggccctcg ggaggccgga ggatgcggtg gaggccttcg
     5821 accaggcgct ggacatcgac ccctcccgcc ccgacgcctg gttccaggaa ggcctggtcc
     5881 tcgagcagtt cggcgagtat aaccgcgcgg tatcggccct cgagacctcc ctctccctcg
     5941 acccgaacca ggccgaggcc tggctccaca tcgcccggtc ccgctcggcg caggggcggc
     6001 ccgctgacgc cgtggcggcc tgcggccagg tgatccggat cgacccgcgt cactcgccgg
     6061 ccctcaccct gaagggcctc tccgaggagc agctcggaga gggggagcag gccctccagt
     6121 cctttgccac gagcctcggt atcgatcccg agcagccgga ggcctggcac caccgcggct
     6181 cggtcctcct ctccctcagg aggttcgcgg aggccgtgga ttccctggac cggtccctca
     6241 ccctcgcgcc ccggaacgga aaggccctct acgagaaggg tatcgcgtat atcgggacgg
     6301 gggccacgga ggaggccgtg acggccctcg gggcgagcct ggatctcgag ccggagaacg
     6361 ctgacgggtg gtaccgccgg gggacagccc tcgcctccct cggcaggcag gccgatgcca
     6421 tcggggacct ggaccggtcc ctcgccctgg acccgggaaa tcccctcgtg tggcttgaga
     6481 agggcctcgc cctgcaggcc atggggaacc tccccggcgc gatggaggcg ttccgggcct
     6541 ccatcgatgc agacccctcc ctgtcccggc cctggctcct gctgggggtt gccgcgaggc
     6601 tctcgggccg gatgggggac gccctcacag ccctcgaccg ggccacccgg gcggatcccg
     6661 aggatcccga gggatggcac gagcgggggc ttgtcctcat ggaactgggc cggaccgagg
     6721 aggggatctc ctccttcgac cgggccctcg ggatccggcc ggaccatgcc gggtctctct
     6781 actcccgcgc cctcgccctc gagatcctgg gaaggcacga ggaggccctc ggttccatgg
     6841 agagggcgct cccctcgaag gagtcggatc ccgcggcatg gttccaccac ggctccctcc
     6901 ttttcaggct ctcgagggac gaggaagcgg cccggtcgtt tgaccggtcc cttgcccttg
     6961 accccgggca gatctccgcc tggttccagc ggggcgagac cctcgagcgg cttggccggt
     7021 tcgagacagc cctcgaatcc ttcgatcggg cggtccgcct cgacccgggt ctcaccggcg
     7081 gattctacca catgggcatc tgccgggaac ggctggggga ccatgcgggg gccgcggagg
     7141 ccttcttccg ggtcctcgag tccgagccgg agaaccccgg ggccctctac catcgcggac
     7201 tcgccctcat ggggacccgg aattaccccg aggctgcggg atccctcgag aaggccatcg
     7261 ccctgtcacc ttcggccgat gcctggtacc agctcggggt cgccagggag aggctcggca
     7321 ggttcagcga tgcggtggag gccctcgacc aggccctcgc gaccgacccg gaccgggctc
     7381 cggcctggtg cctccggggg gtcgccctgg caaagggcgg ggatcatgca ggggccatcg
     7441 gctccttcga gaagtccctc gccctcgaac ccgggaacgg gcgggcatgg ttcggcaggg
     7501 ggtactccct ccaggtgctc ggccggctcc cggaggcaaa ggaggccatc gagaaggcga
     7561 tggacctctc gtacaccggc gccccggtcc ttctcagata cggcctggtc cttgaggacc
     7621 tgggggacca tgacggtgct tcccgctcct tcacccgggt ggtggagctc gatcccggtt
     7681 ccggggacgg cttcctccac ctcggactcg ccgagttcgc cctcgggaag gccggggagg
     7741 ctgccctcgc cttcgaaaag gcggcgaaga tccaggggag gccgagccct gcatggttcc
     7801 acctgggccg ggcgtacctg tccctcggga aggacgagga tgccctcggt cccctcgggg
     7861 aggccgagcg gctcgagccc gacaacgccg aggcctggtt cctcgagggt ctcgccctcc
     7921 accggctcgg ccggttcaag aaggccgtta tatccctgga gaaggccgtc cggctcaacc
     7981 cctcccatgc ccgggcatgg taccagctgg ggcttgcccg ggcaaccctc gatgaccagg
     8041 gcgaggccgc ccgggccttc gggcgggcga cggatctcga cccgtcctcc ggcccggcgt
     8101 ggtacgggct gggggccgcc tcccgacgaa tcggagagaa ccagcgggcc ctggacgccc
     8161 tcgatcacgc cgtctccctg gacccggccg caccggccgg cttgatggag agggggctca
     8221 ccctcgggaa cctcgggcgg tacgcggagg ccctttcagc cttcgaccgg gtgctcgaga
     8281 tcgctcccgg ggataaaccc gccctcatcg cccgggtgga gaccctcgcc tccctggaac
     8341 ggttcgggga ggggctcgcc gcggcccaga ggatgctcga ccgggaccca tccctcgcgg
     8401 cggcctggta cctcaggggc cggtgtctct cggggctggg ccggcacggt gaagcggtgg
     8461 agtccctcgg gagggccctc gagctcgacc cggaccaggc ctccgcctgg caccagctgg
     8521 gccttgtcca ggaggcacgg ggcttcttcg gggacgcggc cgatgctttc tggagggccg
     8581 ggcagatagc gccggaggaa gggtcgtcct ggctc
//
LOCUS       DATGDI010000005        11274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_320212, whole genome shotgun sequence.
ACCESSION   DATGDI010000005 DATGDI010000000
VERSION     DATGDI010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 11274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11274
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320212"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..2673
                     /locus_tag="VKO45_00120"
     CDS             166..2673
                     /locus_tag="VKO45_00120"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMA04317.1"
                     /translation="MWSDFRDYSVYNYIDVYLLNLTPPPEVKKLVQTPSSNSPQPDID
                     GDLIAWVDYPNIFLYDITDASPPASLTSAGVNYGPRISGNRVTYYRDDGRIYLTTLDS
                     SEPCPAASFTANVTSGLANLAVQFNDTSGDPNTTHWSWDFGDGTGSYEKDPVHVYILD
                     GIYDVSLTVGNDVGRDYASNTRYIRVGPLPVVSFSANRTYGITPLPVRFTDTSSGSPT
                     GWSWDFGDEITSTDQNPVHVYGSPGVYTVNLTVTNARGNTSGNIPGFIRALNGTSLVA
                     STPVAGLQVTAVGGMQQVSLNVTDAMGYNATWLAFAPPPSSGWERMDFFSTDGIGFVP
                     SGGVIRGNITSCTLESLDIIPHSFGTGVGNNLPLSYRLDLSAYPVDAGILATIWEGVI
                     PEDNATFRSTLAETSTNFTSVLDIAYTLDFATRNLTGVQSATLNLSVSGAWVTKYGNQ
                     NNIAVVRLGDNLVNQTLNPSATFTDTATNLDFFTVSSPHGLSRFALVSAVGSSNLIQM
                     GARVATQLIQSAASGSSGGSSPVTPRPTAQPAKVPVERQPATFYGEGKIDTTSAGITR
                     DLVTIMSEDRGASLAVKGSTKAFDGTHGPLTLVTVRPVQRDSLPPVPAETGIRFTGIA
                     YDVGPDGATFDPPARISFTVPDGQWSQDTLYFIRTYSPGIGSWNDIPTSVDPGTRIVS
                     GEVSHLCIFGLFASVAPPVSIPPAERAPAAGPKPLPRTPMGTFTGMLGWLYDTMTAHI
                     QVSFLILLGGFASVYAWTRRAWLARYRTWITLYLASLTGLLWAAFRVSQGGLLPEAIF
                     ISTTVVGLNLIVHIFRFDRIHLLPRAGPVTRMPARRW"
     gene            2681..3652
                     /locus_tag="VKO45_00125"
     CDS             2681..3652
                     /locus_tag="VKO45_00125"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMA04318.1"
                     /translation="MRLPLLFQNREPDIPALQAAGDTRELIGLLSHPNPDTRWRAAEA
                     LGTLGGPAVDDLIRELHRHDPQVRLGVVEALGAIGDPRAAPSLLGILSGDGSEEVRWA
                     AALALGDTGDASAVPALAAALRDPSKYVRFGAAVALGNLGWEPAGGEEEACLLIARQD
                     WASLPALGAEAVAPLIRASRDGDPSIRAQAVGILGEVGGPGSGEVCDGVLRDPDGEVR
                     WRATLAFPRCGIPLIRLPMGLARRARPGKSPAVAAFLNLLFLGLGYNYLGRWYGFLLF
                     QLNLTAIVLASLVLGPLIPYAASYALSTPIAVQTWYLAQRSEEAGQA"
     gene            3666..4625
                     /locus_tag="VKO45_00130"
     CDS             3666..4625
                     /locus_tag="VKO45_00130"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMA04319.1"
                     /translation="MNLPSFLRPTPPDIEAMKAGRDIPGLMAALRHPDTGIQWRAAAA
                     LGSFGPEAIRHLTGRLPTRDRSVRLGIIEALGEIRDPLAVDPLLPYLQDPSVEVRWET
                     ALALGEIGDTRAIGPLLPGLRDPDRYVRYGTALALQRLGWTPAGDEEEAYRLLGLQDW
                     AGLAALGPPAIPALGLALKDRERTIRRRSVEVLGQIGNGAAVPAILRGLRDPDDEVRW
                     QAVLAGPRCGIAPMYLPRGLSQRPRTRKNPAIAGFLNLILPGQGYLYLGRWWGILIFQ
                     ADITVTLWLFATTGDQPTYGLLLPLYVLLAAHAWYMAGKMPEM"
     gene            4824..5411
                     /locus_tag="VKO45_00135"
     CDS             4824..5411
                     /locus_tag="VKO45_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04320.1"
                     /translation="MEFELHPSITLESGTFSVVTAPEEPVLRGLNGTPALSRFLFLYV
                     CGNYSRLLTGIKRRSAHFEVRRAFTAYQLLTILREAHHTILFIEHDPSLYEDAGSWSR
                     GAPSPLDGPGSLRDPGTLLPEVAGALAEAAREALVVLYAPRPDPALKVLARRADRVFC
                     LEQGDRGPDRTGRPGPRGGDQTTLEVFGWAAPSRA"
     gene            5387..5626
                     /locus_tag="VKO45_00140"
     CDS             5387..5626
                     /locus_tag="VKO45_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04321.1"
                     /translation="MGRSFESVRMGVAEIAERWLRARRALRKEDQAYAERLAEMAKRH
                     ASEGFYAFDDPLEAAVFSVAIEILRELDGGGDVDP"
     gene            5613..7586
                     /locus_tag="VKO45_00145"
     CDS             5613..7586
                     /locus_tag="VKO45_00145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B DNA-directed DNA polymerase"
                     /protein_id="HMA04322.1"
                     /translation="MWILDTSSRGRVLAWGCRGEGKRELPTVPSFLLHLPDPHRHREL
                     LEGLADRYPVGECTFQTVYGPLCGYRVAAGREVAEQIESQTRCAADLYNVDVRLDQRA
                     LAEAGLVPCSYPGESRFALDFPVPLQAVELEALGNPYRDGPLPPLEVRSEGRREVLEG
                     PERVLLEDFFSLVAALDPDVVLMPCADIWVRRIMDRARALGAEQTLSRSGRFRSLAGK
                     SYWSYGRVYHKDSALVPEGRVVIDTERSFVYREGGLPGVLMASRLSGLPLNLTSRFTP
                     GTLISSYEVYEALRRGIAVPFRKRDPEVPRGSSELAACDKGGMIFQPHPGVYPEVHEL
                     DFTSLYPAIIVKYNLSPEVPDGKGREGFLPSVLAPLLDMRIRSKQLKKTSPAYAGVDS
                     VLKWMLVTCFGYTGYKNAKFGRIEVHEGITSRSREILLATKEIAEGMDLSVLHGMVDC
                     VWVQGEGIGEFKERVEGTVGIPTELDTYSWVTFLPLADGNGAYNRYYGRMTGGEMKVR
                     GVAARRGDTPTYIRRMQEEMIGAMGDAADLEGLRRLDPAIRRIHLRYAEGLPGADPKE
                     MVIRRRVSRLSYGRRSPEAAAVAALRALGAPLAPGMEIGYVVTDASRWLVDVEGEGEG
                     FDTGYYAHLLEKAWDEVAFTLDRVAGPARETRL"
     gene            complement(7751..8149)
                     /locus_tag="VKO45_00150"
     CDS             complement(7751..8149)
                     /locus_tag="VKO45_00150"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMA04323.1"
                     /translation="MIHFEIPSQDPERLAGFYRKLFGWEITNWGPVEYWLATTGPDSE
                     PGINGAIARRDLREGTRNTVSVKSADDTLGMVARMGGKVLSPKMTVPGVGHHALCKDP
                     EGNIFGIMEMDLSAKWTPEEEEIIRKGGRL"
     gene            8288..8920
                     /locus_tag="VKO45_00155"
     CDS             8288..8920
                     /locus_tag="VKO45_00155"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HMA04324.1"
                     /translation="MTGDREAGGTGRISGLTKGRMENLTDGIFAFAMTLLVTTLDFPS
                     TDASLPPLTPQMLVATYFPDFLNFFIAFVVLAGFWMAHHALFRHIRFIDRPLLWMNVL
                     SLLFVALLPFSTDLAGDYPEVPLAAVVFEANLLAIGLFLSLIWNYATTDRRFVEKDLD
                     EGIVRVHARRGLVIPGVSALGILLALLGLHWTTLIYIATPLFFLVTGREG"
     gene            9016..9504
                     /locus_tag="VKO45_00160"
     CDS             9016..9504
                     /locus_tag="VKO45_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMA04325.1"
                     /translation="MMNLFRGGEAPWIRKGFDLLMMKKHGEALETFARGLQEEPKAPL
                     GWLGRGIALSQMGDDRKALEFFDYALRLAPTLGRGWFRKGLSLARLGRHEQALSCFEK
                     VLKFDPSDARAWYQKGLSCEALERYRDAFDCLDQAVRFSDDREIRERRDAVLSLVQQG
                     GR"
     gene            complement(9630..10316)
                     /locus_tag="VKO45_00165"
     CDS             complement(9630..10316)
                     /locus_tag="VKO45_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HMA04326.1"
                     /translation="MITDILAFLIHIDDHLASLVGTYGLWIYLILFLIIFIETGLVVT
                     PFLPGDSLLFVTGALAGGGMLDIVTLSLLLTIAAIGGDSVNYWIGKSSGLERLQCRYP
                     KLFRKEYFETTSRFFERYGGKTIVIARFVPYIRTFAPFMAGVGRMSYPKFLSYNILGG
                     IAWILSFLLAGYFFGGIPVVQEHFDLVVWAIIIISVIAVISILLGVVRFATSEDRCPV
                     EENPGSGERK"
     gene            complement(10488..10655)
                     /locus_tag="VKO45_00170"
     CDS             complement(10488..10655)
                     /locus_tag="VKO45_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HMA04327.1"
                     /translation="MEDRRMRGPRSFGPREMTKTICSDCGKECEVPFKPTEGRPVYCR
                     ECLPKHRKPRF"
     gene            complement(10826..11137)
                     /locus_tag="VKO45_00175"
     CDS             complement(10826..11137)
                     /locus_tag="VKO45_00175"
                     /inference="COORDINATES: protein motif:HMM:NF022420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2769 domain-containing protein"
                     /protein_id="HMA04328.1"
                     /translation="MDKFEMAMQNMAKVSPQEGMKMLQALKGKCICGQCPTYTPAARA
                     AGEGFFCGTGKSFGHITTEVNCMCGKCPNKAELGLKYGFFCNRGSEKALRYDQSMAKK
                     G"
BASE COUNT         1805 a         3890 c         3555 g         2024 t
ORIGIN      
        1 atctgggtaa acgggactga cccagggaac ggcaccgttg gtgtagacat cttcatgaag
       61 gatctcacca cgggtactga gaatcttatc gttgagagtc tgtgcacgtc cccgccgcaa
      121 ttcacaccct gcatcatgga cccccagata tccggggagc gggttgtgtg gtccgatttc
      181 cgtgactatt ccgtgtataa ttacatcgat gtctatctcc tgaatcttac accaccacct
      241 gaagttaaga agctggtcca gactccctcg tccaattcac cccaaccgga tatcgacggt
      301 gatctcatcg cctgggtgga ctatccgaat atcttcctct acgatatcac agatgcctca
      361 cctcccgcca gcctcacctc tgccggcgtg aactacggcc ccaggatatc ggggaaccgg
      421 gttacctatt accgcgacga cgggagaatt tacctcacca cccttgactc atcggaaccc
      481 tgtcctgccg caagcttcac cgcaaatgtg acctccggcc tggcgaacct ggccgtgcag
      541 ttcaacgata cgtcgggcga cccgaacacc acccactggt cctgggactt cggggacggg
      601 accgggtcat acgaaaagga cccggttcac gtctatattt tagacggtat ctacgacgtg
      661 tcactcaccg tcgggaacga cgtagggagg gattacgcat cgaacacccg ctacatccgg
      721 gtcgggcctc tccccgtggt ttccttctcg gcgaaccgga cctacgggat cacgcccctc
      781 cccgtccggt tcaccgatac ctcgtcaggg agccccaccg gctggtcgtg ggacttcggc
      841 gatgagatca cctcgacgga ccagaacccg gtccatgtct atggatctcc gggagtgtac
      901 acggtgaacc tcaccgtgac gaatgcccgc gggaatacct cggggaacat ccccggtttc
      961 atccgggccc tgaacgggac gtccctcgtc gcgagtactc ccgtggcggg gcttcaggtc
     1021 acggccgtcg gtgggatgca gcaggtaagc ctcaacgtca ccgatgcgat gggctacaat
     1081 gccacatggc tcgccttcgc cccgccccct tccagcggct gggagaggat ggactttttc
     1141 tccacggacg ggatcgggtt cgtgccgtcc gggggtgtta tccggggaaa catcacctcc
     1201 tgcacccttg agagcctcga tatcattccc cacagtttcg ggaccggcgt cgggaataac
     1261 ctccccctgt cctatcgcct ggatctttcc gcgtatcccg tggacgccgg aattctcgcc
     1321 acgatctggg agggggtgat cccggaggac aatgcgacct tcaggagcac cctcgccgag
     1381 acgagcacga acttcacgag cgtgctcgat atcgcgtata ccctggactt tgccacccgg
     1441 aacctcaccg gtgtccagag cgccaccctg aacctctcgg tgagcggggc atgggtgacg
     1501 aagtacggca accagaacaa catcgccgtc gtccgccttg gcgacaacct ggtgaaccag
     1561 accctcaacc catcagccac cttcactgat accgccacga atcttgattt cttcaccgtg
     1621 agttcccccc acggcctctc ccggttcgcc ctcgtctccg ccgttggatc ctcgaacctg
     1681 atccagatgg gtgcccgggt cgccacccag ctgatccaga gcgcggcttc cggttcaagc
     1741 ggcggcagtt ccccggtgac cccgaggcca accgcccagc cggccaaggt tccggtagaa
     1801 cgccagccgg cgacctttta cggggagggg aaaatcgaca cgacctctgc cggtatcacc
     1861 cgcgacctgg tgaccatcat gtcagaggac cggggggcaa gcctcgccgt caagggtagc
     1921 acaaaggcct ttgatggtac ccacgggccc ctcaccctcg tgaccgtgag gcctgtccag
     1981 agggattccc ttccccctgt tcccgcagaa acgggaatcc gcttcaccgg gatcgcgtat
     2041 gatgtggggc cggatggcgc gaccttcgac ccgcctgcca ggatcagctt taccgtcccc
     2101 gacggccaat ggagccagga taccctgtat ttcatccgca cctattcgcc ggggatcggg
     2161 tcctggaacg atatccccac ctcggtggat cccggtaccc gcatcgtgag cggggaggta
     2221 tcacacctct gcatcttcgg gctctttgcc tccgtggcac cccctgtgtc catcccccct
     2281 gccgaaaggg ctccagcggc cggtcccaag cccctcccgc gaacccccat gggcaccttc
     2341 accggcatgc tcggatggct ctatgacacc atgacggccc atatccaggt ctccttcctg
     2401 atcctcctcg gggggttcgc ctctgtctac gcctggaccc ggagggcatg gctcgcccgg
     2461 taccggacct ggatcaccct gtaccttgcc agcctgaccg gactcctctg ggcggccttc
     2521 cgcgtgagcc agggcgggct cctcccggag gcgatcttca tctccaccac cgtcgtcggc
     2581 ctgaacctca tcgtccatat cttccggttc gaccgcatcc atctccttcc ccgggcaggg
     2641 cccgtcaccc ggatgccggc caggcggtgg taaccccggg atgcgcctcc ccctcctgtt
     2701 ccaaaaccgg gaacccgata ttccggccct gcaggccgcc ggtgataccc gtgagctcat
     2761 cgggctcctc tcccatccca accccgatac ccggtggagg gcggccgagg ccctcgggac
     2821 cctcggaggt cctgccgtcg acgacctgat ccgggagctc catcgccacg atccgcaggt
     2881 ccggctcgga gtcgtcgaag ccctcggagc catcggggac ccccgcgcgg ctccttccct
     2941 cctcgggatc ctctcgggag acgggagcga ggaggtccgc tgggccgccg ccctcgccct
     3001 cggggacacc ggcgatgcct cggccgtccc tgccctcgcg gccgcgctcc gggatcccag
     3061 caagtacgtc agattcgggg cggcggttgc cctcgggaac ctcgggtggg aacctgcggg
     3121 gggggaggag gaggcctgtc ttctcatcgc ccggcaggac tgggcctccc tcccggccct
     3181 gggggcggag gccgtggcgc ccctcatccg ggccagccgg gacggcgatc cctccatccg
     3241 ggcgcaggcc gtcgggatcc tcggggaggt cggcgggccg ggatccgggg aggtgtgtga
     3301 tggcgtcctc cgggaccccg atggcgaggt ccggtggcgg gcgaccctgg ccttcccgag
     3361 gtgcgggatc cccctcattc gcctccccat gggccttgcc aggcgggcga ggcccgggaa
     3421 gagcccggcc gtcgcggcct tcctgaacct cctcttcctc ggcctcgggt acaactacct
     3481 gggccggtgg tacgggttcc tcctcttcca gctgaacctg accgccatcg tcctcgcctc
     3541 cctcgtcctc gggcccctca ttccctatgc cgcctcctat gccctttcca cgcccatcgc
     3601 cgtgcagacc tggtacctgg cacagagatc cgaggaggcc gggcaggcat gaaggaggca
     3661 tccccgtgaa cctcccctcc ttcctccggc ccacccctcc ggacatcgag gccatgaagg
     3721 ccgggaggga catccccggc ctcatggcgg ccctccggca cccggatacc ggtatccagt
     3781 ggagggcggc ggcggccctc ggatccttcg gcccggaggc catccgccat ctcaccggac
     3841 ggctcccgac ccgggaccga tcggtgaggc tggggatcat cgaggccctc ggagagatcc
     3901 gggatcccct ggcggtggat cccctccttc cctacctcca ggacccgagc gtggaggtcc
     3961 ggtgggagac cgcccttgcc ctcggagaga tcggcgatac acgggccatc ggccccctcc
     4021 tccccggact ccgggatccc gaccggtacg tccggtatgg caccgccctc gccctccaga
     4081 gactgggctg gaccccggcg ggggacgagg aggaggccta ccggctcctc ggcctccagg
     4141 actgggcggg gctcgccgcc ctcgggcccc cggccatccc cgccctcggc ctcgccctga
     4201 aggaccggga gagaacgatc cggaggagat ccgtggaggt cctcggccag atcgggaacg
     4261 gggcggccgt cccggccatc ctccgcggcc tccgggaccc cgacgatgag gtgcgatggc
     4321 aggccgtcct tgcgggcccg agatgcggga tcgcgcccat gtacctcccc cggggactct
     4381 cgcagcgccc ccggacccgg aagaaccccg ccatcgccgg tttcctcaac ctcattctcc
     4441 ccggccaggg ctacctctac ctggggcgct ggtgggggat cctcatcttc caggcggaca
     4501 tcaccgtgac cctctggctc ttcgccacca ccggcgacca gcccacctac ggcctcctcc
     4561 tccccctcta tgtcctgctc gccgcccatg catggtacat ggcgggaaag atgcccgaga
     4621 tgtgaaggga tcgggcatcc ggcccacccg aaacatggcc ggggacgaag cctcttctgc
     4681 gagccggcgg gtaccgcaaa acccgcccct ggctcggaac accgggatac gcccgtttca
     4741 cacggattct tccgggggtc gcggcacttt cgcccctcac gctctcatgg gtttatgatg
     4801 gcacttcgcc accactcatc tccatggagt tcgagctgca cccgtcgatc accctcgaga
     4861 gcggcacctt ctcggtcgtg acggcaccgg aggagcccgt gctccggggg ctgaacggga
     4921 cacccgccct ctcccggttc ctcttcctct atgtctgcgg gaactactcc cggctcctca
     4981 ccgggataaa gcgccgctcg gcccacttcg aggtgcggcg ggccttcacc gcttaccagc
     5041 tcctcaccat cctccgggag gcccaccaca ccatcctctt catcgagcac gacccctccc
     5101 tctacgagga cgccggatcc tggtccagag gggccccttc ccccctggac ggtcccggct
     5161 cccttcggga cccggggacc ctcctccccg aggtggcggg ggccctcgcc gaggcggcgc
     5221 gggaggccct cgtcgtgctc tacgcccccc ggccggaccc ggccctcaag gtcctcgccc
     5281 ggcgggccga ccgggtcttc tgcctcgagc agggggaccg ggggccggac cggaccgggc
     5341 gcccggggcc ccggggaggg gaccagacca ccctggaggt gttcggatgg gccgctcctt
     5401 cgagagcgtg aggatgggcg ttgcggagat cgccgagcgg tggctccggg cgaggcgggc
     5461 cctcaggaag gaggaccagg cctatgccga gcggctcgcg gagatggcga agcgccacgc
     5521 gagcgagggg ttctatgcct tcgacgaccc cctcgaggcc gcggtcttct cggtggccat
     5581 cgagatcctg agggagctgg acggcggcgg cgatgtggat ccttgacacc tcctcccggg
     5641 gccgggtcct cgcctggggc tgccgggggg aggggaagcg ggagctcccc accgtcccgt
     5701 ccttcctcct ccatctcccc gatccccacc ggcaccggga gctcctggag ggcctcgcgg
     5761 accggtaccc ggtcggggag tgtaccttcc agacggtcta cggccccctc tgcggctacc
     5821 gggtcgcggc gggcagggag gtcgcggagc agatcgagag ccagacccgc tgcgccgccg
     5881 acctctacaa cgtggatgtc cggctcgacc agcgggccct cgcagaggcc ggcctcgtcc
     5941 cctgcagcta ccccggcgag tcccggttcg ccctcgattt ccccgtgccc ctccaggcgg
     6001 ttgagctcga ggccctgggg aacccctacc gggacggccc cctcccgccc ctcgaggtgc
     6061 ggagcgaggg gagacgtgaa gtgctcgagg gaccggagcg ggtcctcctc gaggacttct
     6121 tctccctcgt cgcggccctc gaccccgacg tggtcctcat gccctgtgcg gatatctggg
     6181 tccgccggat catggaccgg gcccgggccc tcggggccga gcagaccctg agccggagcg
     6241 ggcggttccg gtccctcgcc gggaagtcct actggagcta cgggcgcgtg taccacaagg
     6301 actccgccct cgtccccgag gggagggtgg ttatcgacac cgagcggtcc ttcgtgtacc
     6361 gggagggggg gctccccggt gtcctcatgg cctcccggct gtcgggcctg cctctcaatc
     6421 tcacctcccg gttcacgccg ggtaccctca tctcctcgta cgaggtgtac gaggccctcc
     6481 ggcgggggat cgccgttccc ttccggaagc gggacccgga ggtcccgagg ggctcctccg
     6541 agctcgctgc ctgcgacaag ggggggatga tcttccagcc gcaccccggg gtgtacccgg
     6601 aggtccacga gctggacttc acctccctct accccgccat catcgtgaag tacaacctgt
     6661 cgccggaggt cccggacggg aaggggaggg aggggttcct cccgtcggtg ctcgcgcccc
     6721 tcctcgacat gcggatccgg tcgaagcagc tgaagaagac ctccccggcc tacgcggggg
     6781 tggactcggt cctcaagtgg atgctcgtca cctgcttcgg gtacacgggc tacaagaacg
     6841 ccaagttcgg gaggatcgag gtccacgagg ggatcacctc ccggtcccgg gagatcctcc
     6901 tcgcgaccaa ggagatcgcc gaggggatgg atctctccgt cctccacggg atggtggact
     6961 gcgtctgggt gcagggggag ggaatcggcg agttcaagga gcgggtggag gggacggtcg
     7021 gcatccccac cgagctcgac acgtacagct gggtcacctt cctccccctc gcagacggga
     7081 acggtgccta caaccggtac tacgggcgga tgaccggggg ggagatgaag gtgcgggggg
     7141 tcgcggcccg gcggggcgat acccccacct acatccggcg gatgcaggag gagatgatcg
     7201 gggcgatggg ggatgcggct gacctggagg ggctccggag gctcgacccc gccatccggc
     7261 ggatccacct gcgctacgca gaggggctcc ccggggccga cccgaaggag atggtcatcc
     7321 gcaggcgagt gagccggctc tcctatggcc ggaggtcacc ggaggccgcg gcggtcgcgg
     7381 ccctccgggc cctgggggcg cccctcgccc cgggcatgga gatcggctac gtggtgacgg
     7441 atgcgtcccg ctggctcgtg gatgtggagg gagagggaga ggggttcgat accggctact
     7501 acgcccacct cctggagaag gcctgggacg aggtggcctt caccctggac cgggtcgcgg
     7561 gtcccgctag ggagacccgg ctctgaagga gccggggctg tcccgggggg taggggcccc
     7621 tcgggactaa gccgggaccg tccagggggg aggaggcgcg aagcggctcc tcctgtccag
     7681 gggggtaggg gcccctctgg actcgggtag gggcccctct ggaccgggtc gcggcaggag
     7741 aggccggatc ctagagcctc cctcccttcc tgatgatctc ctcctcctcc ggcgtccact
     7801 ttgcggagag gtccatctcc atgatcccga agatgttccc ctcggggtcc ttgcagaggg
     7861 catggtgccc cacaccgggc acggtcatct tcgggctgag caccttcccg cccattcttg
     7921 ccaccatgcc gagggtgtca tcggcggact tcacgctcac ggtgttccgg gtgccctcgc
     7981 gaaggtcccg gcgtgcgatg gcgccgttga tgcccggttc cgagtcaggg cccgtggtcg
     8041 cgagccagta ttcgaccggg ccccagttcg tgatctccca tccgaagagt tttcggtaga
     8101 agcctgcaag gcgttcggga tcctgcgagg ggatctcaaa gtgtatcact cgggacatga
     8161 tggttcgacc cgttccaacc cctgctgcat ttcctgataa gccttgctgt tgcaccgtgc
     8221 accgggggag gggggcccgg aagagtgaga aggtttattc ccccgtgaaa aaaagcccgg
     8281 tatacagatg accggggacc gggaggcagg aggaacgggc cggatcagcg gcctcaccaa
     8341 ggggcggatg gagaacctca ccgacgggat cttcgccttc gccatgactc tcctcgtcac
     8401 caccctggac ttcccgagta cagacgcctc cctccccccg ctcacccccc agatgctggt
     8461 cgcgacctat ttccccgatt tcctgaactt cttcatcgcc ttcgtcgtcc tcgccggctt
     8521 ctggatggcc caccacgccc tcttccggca catccggttc atcgatcggc ccctgctctg
     8581 gatgaacgtc ctctccctcc tcttcgttgc cctcctcccc ttttccaccg acctcgccgg
     8641 ggactacccg gaggtccccc tggccgctgt ggtgttcgag gcaaatctcc tcgccatcgg
     8701 ccttttcctc tccctcatct ggaactatgc cacgaccgac cggcggttcg tggagaagga
     8761 cctggacgag gggatcgtgc gggtccatgc ccggaggggc ctcgtcatcc ccggagtctc
     8821 ggccctcggg atccttctcg ccctcctcgg ccttcactgg acgaccctga tctacatcgc
     8881 gacccccctc ttcttcctgg tcaccgggcg ggaaggctaa aaccggaccc tttccggggt
     8941 ttttccggga gaaaatattt gggctctccg catgcacctc tccctggaaa atcccagaaa
     9001 cggaggtgat catggatgat gaacctcttc aggggcggcg aggccccctg gatccggaag
     9061 ggcttcgacc tgctcatgat gaagaagcac ggcgaggccc tcgagacctt tgcccggggg
     9121 ctccaggagg agccgaaggc ccccctgggg tggctcggga ggggaatcgc cctctcccag
     9181 atgggggacg accggaaggc gctcgagttc ttcgactacg ccctccggct ggcccccacc
     9241 ctgggccggg gatggttcag gaagggactc tccctcgccc ggctcggccg gcacgagcag
     9301 gcactttcct gcttcgaaaa ggtcctgaag ttcgacccgt cagacgcccg ggcctggtat
     9361 cagaagggcc tttcctgcga ggcgctcgag cggtaccgcg atgccttcga ctgcctcgat
     9421 caggcagtcc ggttctcgga tgaccgggag atccgggagc ggcgcgatgc ggttctctcc
     9481 ctggtccagc agggaggccg ttagggcggg cgggttcccg tgagacccgg gacatccggg
     9541 gctgtcctct cctcccgcca ccggggcggg attgatgctc tcgatctgtc cggccccggc
     9601 ctttcctttg cctcccgcgg caccttcccc tattttctct cccctgagcc ggggttctcc
     9661 tcaacgggac accggtcctc tgaggttgcg aacctgacga cgccgaggag gatggagatg
     9721 accgcaataa ccgagatgat gatgatggcc cagaccacga ggtcgaagtg ctcctgcacg
     9781 accgggatgc caccgaagaa gtacccggcg aggaggaagg agaggatcca ggcgatccct
     9841 ccgaggatgt tataggagag gaacttcggg tagctcatcc tgccgacgcc cgccatgaag
     9901 ggggcgaagg tccggatgta cgggacgaag cgggcgatga cgatggtctt ccctccatac
     9961 ctctcgaaga accggctcgt ggtctcgaag tactcctttc ggaagagctt ggggtagcgg
    10021 cactgcagcc gttcgagccc cgatgacttc ccgatccagt agttcaccga gtcccccccg
    10081 atggccgcga tggtgaggag gagggagagg gtgacgatgt cgagcatccc cccgccggcg
    10141 agggcccccg tgacaaagag gagggagtcc cccgggagga agggggtgac aacgaggccg
    10201 gtctcgatga agatgatgag gaagaggatg aggtagatcc acaggccgta ggtgccgacg
    10261 agggaggcca ggtgatcgtc gatatggatg agaaaagcga ggatatccgt gatcatgaat
    10321 gctcaccggt tgggtggcat gctataaatg cttcacgagc cttttcccgc cctctccggc
    10381 ggacaggttc cgttgcctcc cctcgcccgt tgcggggaac gggatcctcc ggtcacccgt
    10441 gaagatctcc cgctcgggaa aaagggaagt tgtgaagatt tcgatggtta gaatcgcggc
    10501 ttccggtgct tcggaaggca ctcgcgacag tagacggggc gcccctcagt tggcttgaac
    10561 ggaacctcgc actcttttcc acagtccgaa cagatggttt ttgtcatttc cctgggccca
    10621 aagctgcgcg ggcctctcat tcttctgtct tccattgttt ttcctttgca gtctacgcga
    10681 aaactgcatt taaaagatgg agaaaatgac tatatagtct tttcttgccg gggattgtcc
    10741 cggaaaaagt ccgccggtgg aggatcccgc ggggctcgcc cgccacgcgg aacaccctgg
    10801 atggaagggg accgtggggg ctggatcaac ccttcttcgc catgctctgg tcgtacctga
    10861 gggccttctc cgagccacgg ttgcagaaga agccgtattt gagccccagc tcggccttgt
    10921 tcgggcattt accgcacatg cagttcacct cggtggtgat gtgcccgaag ctcttccctg
    10981 tgccgcagaa gaatccctcg ccggccgccc ttgcggcggg agtatacgtc gggcactggc
    11041 cgcagatgca ctttcccttc agggcctgga gcatcttcat cccttcctgg ggggacacct
    11101 tcgccatgtt ctgcatggcc atctcgaact tatccatatc gcaccacccc ttccttcatg
    11161 gagcgattat tttaatttta tgagtattct caagaattgg agggatttct cctggtttgc
    11221 gcgggatggt cgcgggggtc gcgtcgggag aacggggaac gatagctata tcgt
//
LOCUS       DATGDI010000006         2927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_636, whole genome shotgun sequence.
ACCESSION   DATGDI010000006 DATGDI010000000
VERSION     DATGDI010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1544)
                     /locus_tag="VKO45_00180"
     CDS             complement(66..1544)
                     /locus_tag="VKO45_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMA04329.1"
                     /translation="MTKESRPFWPPDHSVFRTRSIADLVERSKGEHALRKVLGPFELV
                     LLGVGAIVGTGIFVVTGVAAANYSGPALVVSFAIAGTACVFAALCYAEFATMVPIAGS
                     AYTYGYASLGEIWAWIIGWDLILEYSVSIAAVAIGWSGYMVNLLAGAGITFPAALANP
                     PGVSGGIVNLPAILIIGAITGLLILGIRESATVNNVIVLVKLSVILLFIYLGVSHVNP
                     VNWVPFFPYGWGGVITGAAIVFFAYIGFDAVSTAAEEVRDPQKNLPVGIIGSLLISTV
                     LYIIVSALLTGMVPYLDFRDTSAPVAFALSRIGIGWGAALVSVGAICGITSVILVLLY
                     GQTRIFFAMARDGLLPCTFGTVHPVLRTPVKVTLLVGCATAAIASLLPLTSVAELVNI
                     GTLAAFIIVSAGVLVLRRTRPELPRPFRTPLVPWVPGLCIVACLGLILALPAITHLRF
                     VVWLAIGLAIYFLYGARNAWTTTGNSLEACGGREAARKGPGA"
     gene            complement(1682..2482)
                     /locus_tag="VKO45_00185"
     CDS             complement(1682..2482)
                     /locus_tag="VKO45_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HMA04330.1"
                     /translation="MLRIGCCGFPVGLLRYTHELPVVEVQKAFYRIPGRDTVQRWRDR
                     VPEDFIFTVKTWQGITHPATSPTYRRSGPIPEEMRGLIGHFRPTEPVFQGWRETRDIA
                     RILGAPLILLQAPPSFTETQEHEQNLTEFLSGIDREDIRIALELRASWDRARLASLCR
                     KFDLIHCVDPFGDTPVTGPPFYLRLHGSPPGLRPVGSPSGLRPSGSPPGKRMYAYTYT
                     ETDLQWLRDRIRDWDVEGEVYCLFNNLSMWDDALAFRDLLRDASGRYG"
     gene            complement(2560..2790)
                     /locus_tag="VKO45_00190"
     CDS             complement(2560..2790)
                     /locus_tag="VKO45_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04331.1"
                     /translation="MAGRNKRITTIVALIVVLVAFFQMLWPRLYPWVVSRNPYPTHEY
                     FQIMGFVSPNGLSILAVIIGLIFIAWAAVTDP"
BASE COUNT          620 a          863 c          990 g          454 t
ORIGIN      
        1 cgggctccat gctacgaagg tacgcacctc aaggttgata atacgatccg ggaggcactc
       61 tctcttcagg cgccgggccc cttccgggcg gcctctctcc caccgcaggc ctcgagggag
      121 ttccccgtgg ttgtccaggc attccttgcg ccgtagagga aatagatggc gaggccgatg
      181 gcaagccaga cgacgaacct caggtgggtg atggcgggga gggcgaggat gaggcccagg
      241 caggcgacga tgcagagccc ggggacccag gggacgagag gtgtgcggaa gggtctcggg
      301 agctccggcc gcgtcctcct gagcacgagg actcctgcgg agacgatgat gaaggcggcg
      361 agggtcccga tgttcacgag ctccgcgacg gaggtgaggg ggaggagcga tgcgatggcg
      421 gcggtggcac atcccacgag aagggtcacc ttcaccgggg tccggagaac cgggtgcacc
      481 gtgccgaagg tgcaggggag gagcccgtcc ctcgccatgg caaagaagat ccgggtctgc
      541 ccgtagagga ggaccaggat gaccgaggtg atcccgcaga tcgccccgac cgagacgagg
      601 gcggcccccc agccgatccc gatcctcgac agggcaaagg cgaccggcgc actcgtgtcc
      661 ctgaaatcga ggtacgggac catgcccgtc aggagagcgg agacgatgat gtaaaggaca
      721 gtggagatga ggagggaccc gatgattccc acggggaggt tcttctgggg atcccgcacc
      781 tcctcggcgg cggtggagac ggcgtcgaag ccgatgtagg cgaaaaagac gatggccgcg
      841 cccgtgatga ccccgcccca gccgtagggg aaaaagggaa cccagttcac cgggttcacg
      901 tggctcaccc cgaggtagat gaagaggagg atcaccgaga gcttcacgag cacgatgacg
      961 ttgttgacgg tcgcactctc ccggatcccg aggatgagga gcccggtaat ggccccgatg
     1021 atgaggatgg cagggaggtt cacgataccc ccggagaccc ccggcgggtt cgcgagggcg
     1081 gcggggaagg tgatcccggc acctgcgagc aggtttacca tgtaccccga ccacccgatg
     1141 gcgacggccg cgatggagac cgaatactcg aggatgaggt cccagccgat gatccatgcc
     1201 cagatctccc ccagggacgc gtagccgtag gtgtaggcac tccccgcgat ggggaccatg
     1261 gtcgcgaact cggcgtagca gagggcggca aagacgcagg cagtcccggc gatggcaaag
     1321 gaaaccacga gggcaggccc cgagtagttc gcggccgcga ccccggtgac aacgaagatc
     1381 ccggtcccga cgatggcccc gacgccgagc aggacaagct cgaagggccc gagcaccttc
     1441 cggagggcat gctccccctt tgatcgctcc acgagatcgg cgatggatcg ggtccggaaa
     1501 acggagtgat ccgggggcca gaacggccgg ctctccttcg tcatgcccga cctggcctcc
     1561 ccccctcccg ggtgagagac ccccctcggc ggagctccgt catccggaaa attcccctga
     1621 cgccgggcgg atcgggcaaa ggaggtctca atgccccatt ctcttcctga cacggatata
     1681 gctatccata tcggcctgat gcatcacgca ggagatcacg aaaggccagg gcgtcgtccc
     1741 acatcgagag gttgttgaag aggcagtata cctcgccctc tacatcccag tcccggatcc
     1801 ggtcacgaag ccattgcagg tctgtctctg tgtacgtata ggcgtacatc cgtttcccag
     1861 gcggggagcc cgaagggcgt aagcccgagg gagaaccaac ggggcgaagc cctggtggtg
     1921 acccgtgaag cctcaggtaa aagggcggcc cggtcaccgg ggtgtccccg aagggatcga
     1981 cgcagtggat caggtcgaac ttccggcaca gggaggcgag cctcgccctg tcccaggagg
     2041 cccggagttc gagagcgatc cggatatcct cccgatcgat acccgaaagg aattcggtca
     2101 ggttctgctc gtgttcctgg gtctcggtga aggagggagg ggcctggaga aggatcagag
     2161 gggccccgag gatgcgagcg atatcccgag tctccctcca tccctgaaag accggttctg
     2221 tcggccgaaa gtggccgatc agccctcgca tttcctccgg gatcggtccg gatctccggt
     2281 atgtgggaga tgtcgccggg tgggtaatcc cctgccacgt ctttacggtg aagatgaagt
     2341 cctccgggac cctgtccctc catctctgaa cagtatcccg gccgggtatg cggtagaagg
     2401 ccttctggac ctcgacaacc gggagttcat gggtgtaccg gagaagacca acgggaaacc
     2461 cgcagcaccc gatccgaagc atggtcagtc aggtaaggaa ccgccgggag atgagccttt
     2521 ccaggccagg caaccggagg cagaggcaca gcggaaaatt cagggatcgg tgaccgccgc
     2581 ccatgcgatg aaaatgaggc cgatgatgac cgcgaggata ctgagcccgt tcgggctcac
     2641 gaatcccatg atctggaaat actcatgggt cggatacggg ttacggctca caacccaggg
     2701 gtataaacgg ggccagagca tctggaagaa tgccaccagg acgacaatca gggcgacgat
     2761 ggttgttatc cttttgtttc ttcccgccat ggacggtcac cctcctttgc agatacacag
     2821 gtggatgaaa cgggtataag gagtttctgg aaatgccccc cggacatgcg acggaggagg
     2881 ttccatctga aaaaggggac gccccggctg ggattcgaac ccaggtc
//
LOCUS       DATGDI010000007        10977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_233331, whole genome shotgun sequence.
ACCESSION   DATGDI010000007 DATGDI010000000
VERSION     DATGDI010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 10977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10977
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233331"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VKO45_00195"
     CDS             <1..806
                     /locus_tag="VKO45_00195"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HMA04332.1"
                     /translation="GITTTLGRSGSDYSATVVGAAIGADEVWIWTDVDGIMTTDPRLV
                     REARVLPTVSYLEAMELSYFGAKVMHPRSIEPAMRKEIPIRVKNTFNPSHPGTCITKE
                     KRKSRRVVKALTHITGVALVNVNGAQMIGRPGVARAIFSALADREVNVMMISQGSSEA
                     NISIIVELDHMEIAAEALKPMVEQGIVREVTANPDVAAVAVIGAGMIGTPGISGRIFT
                     ALGLAGVNIMMISQGSSEVNISFVVKESDGPKAVRVLHEELRLSEEQDG"
     gene            799..1791
                     /gene="purM"
                     /locus_tag="VKO45_00200"
     CDS             799..1791
                     /gene="purM"
                     /locus_tag="VKO45_00200"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HMA04333.1"
                     /translation="MGRETYRAAGVDIDLVKDAIGALTGKVTFRRKKPYGMLGGVGHY
                     AGLIDFGEHVLALTTDGVGTKMLVADQLRDWRTVGIDCIAMNANDLFVMNVEPVAFVD
                     YIATDRLSVEKMAQIGEGLNEGARLANLNLVGGETAQLPGLVNGLDLAGTCLGVQEKG
                     KVVSGEKIRPGDCILGVPSSGIHSNGLTLARRVVEKSGGYGERLPGGMTVGKALLTPT
                     RIYRESLEACKKAEVHGMCHVTGSGLLNFTRLTKYGFEFTDPLPVPPVFSWLQERGEI
                     EPVEMYRTFNMGMGFAYILPEEEADILRKFIPGSRVVGEIVEKSGIRMKGLPVR"
     gene            complement(1756..2337)
                     /locus_tag="VKO45_00205"
     CDS             complement(1756..2337)
                     /locus_tag="VKO45_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZPR1 zinc finger domain-containing protein"
                     /protein_id="HMA04334.1"
                     /translation="MRSVVPGRCPYCEREIQYLYQTENIPYFSDILIVSARCECGYRH
                     ADTMVLSGGEPARWELRIEGPEDLNARVVRSSSGTMRIPELGVEIDPGPACEGFVSNV
                     EGVLERIDDVVSGVLTWAEGEERENALVLRRRIELAREGSLPFTLSLEDPTGNSAIIS
                     PRVTKTPVPACVDEDPGESPGSLPDGETLHPDA"
     gene            complement(2338..2715)
                     /gene="sepF"
                     /locus_tag="VKO45_00210"
     CDS             complement(2338..2715)
                     /gene="sepF"
                     /locus_tag="VKO45_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HMA04335.1"
                     /translation="MSKLLDSIIGRSPPTGSDDYMELDLASFEGTMSDQPATMYVKVA
                     TIGDLKDTPKVKEEVYNGNIVIIDIGRLKMDKITYERVLKDLKDVAKDVNGDIVGLGD
                     QRYVVITPMSVKIAREKIGGGSP"
     gene            complement(2834..3331)
                     /locus_tag="VKO45_00215"
     CDS             complement(2834..3331)
                     /locus_tag="VKO45_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HMA04336.1"
                     /translation="MAALTVKKRHTIRKDELAELFTLLGREIGPSAEKFRSDRVEMAE
                     IDAPFSLFLVDKKPLLLKREGWVFPTVRGAIDRPFPERTIVVDMGAVPFVAKGADVMR
                     PGIVSVTPDVRKGSPAIVVDERHRKALAVVVALLDGPAIMAEAKGKMAKTVHHVGDLL
                     WALEL"
     gene            3436..3660
                     /locus_tag="VKO45_00220"
     CDS             3436..3660
                     /locus_tag="VKO45_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain-containing protein"
                     /protein_id="HMA04337.1"
                     /translation="MTKRPMEILDQALNRQPVIVSLKGKREIRGILQGYDVHMNLVLD
                     QAEEVGGEARKLGTLIVRGDNVIYISPIVG"
     gene            3679..3861
                     /locus_tag="VKO45_00225"
     CDS             3679..3861
                     /locus_tag="VKO45_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HMA04338.1"
                     /translation="MSKGTPSKGKRQHKVHIACRRCGHISFHVRHKTCSSCGFGRSAR
                     IRGYRWMNKKPKIPTH"
     gene            3869..5326
                     /gene="purF"
                     /locus_tag="VKO45_00230"
     CDS             3869..5326
                     /gene="purF"
                     /locus_tag="VKO45_00230"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HMA04339.1"
                     /translation="MCGIVGIIDAGGVSRPLYFALFALQHRGQESAGISTWDGTCFYK
                     HKGMGLVSEVFNDAILSELPGQVGVGHVRYPTTGANLPENSQPFNFRFRDHHFAIAYN
                     GNLVNSAELRSEFEQDGEIFSTTTDTEIVAATIVHEFRKSGKIEEAIIHCLHVLRGSY
                     SVVLMLDGILYAFRDPMGIKPLCIGTTRNGFIVASESVAIDTLGGTLIRDVQPGELFR
                     ITGQGFEAMQIAVADHRAHCIFEYIYFARADSVIEGRLVYDVRRKIGASLYDEAPVKA
                     DLVSPVPDSGTSVAVGFSHHSGIPYLEALMKNRYTARTFIKPSQKERELAVRLKLNPI
                     IGHIDGKKVVLVDDSIVRGTTSRHIVELMKESGASEVHVRIGSPQIIAPCYLGVDMPT
                     RAELIASTRPVDEVKESISATSLHHVSLEALVKAIGIDRENLCTGCLTGKYPLFIEGE
                     SCLSRNIDFVVGSYQAKLGSFPQGRTAAKTAGKGK"
     gene            complement(5324..5398)
                     /locus_tag="VKO45_00235"
     tRNA            complement(5324..5398)
                     /locus_tag="VKO45_00235"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5361..5363),aa:Met,seq:cat)
     gene            5505..5939
                     /gene="trxA"
                     /locus_tag="VKO45_00240"
     CDS             5505..5939
                     /gene="trxA"
                     /locus_tag="VKO45_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HMA04340.1"
                     /translation="MDDELQRIREKKLEELKRGIQGKPEKPDRPGATPEGQVMVLDER
                     QFAAAVRDNPCLVVDMWAPWCGPCRMVAPVVQELAAEFAGKIQFAKVNTDENQRVAAT
                     YGITAIPTILLFARGRLVERVIGAYPKPMLRDRILRAFGMTG"
     gene            complement(6122..6463)
                     /locus_tag="VKO45_00245"
     CDS             complement(6122..6463)
                     /locus_tag="VKO45_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HMA04341.1"
                     /translation="MDLAQEKCTPIEPGTPPLTRRKLLSLVKEVPDWALEGTRLNRRF
                     ACKSFVEAVKFLNRVSVIAAEEMHYPEMCITEQRNVTISCYTHASGGITLNDLILAAK
                     ISDAWRVEGAH"
     gene            complement(6625..8214)
                     /locus_tag="VKO45_00250"
     CDS             complement(6625..8214)
                     /locus_tag="VKO45_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HMA04342.1"
                     /translation="MPVDTGTFLAVTGIYVVLTLAVGYLGYRRTKGAEDYMLAGRRAG
                     PWILALSYGATFISTSAIIGFGGVAAVYGMGLVWLTVLNIGMGILVAFLVFGKRTREI
                     GKRLGAVTFPDLMGRRFSDGFMQYASAAVIILGMPLYTSAILIGGARFLEKTIPMPFG
                     WAVIVLGLVTAVYVVAGGLIAVMYTNSLHAIVMFIGMAILLVFTYSLLGGVAAAHEAL
                     TGLAPLIPADLQAQGLTGWTTLPALGSPIWYTLVTTLILGVGIGALAQPQLVVRFMTA
                     KDSRTLDRAVLVGGPFILMMTGVTFTVGALSNVFFYRDTGQIAMAAVAGGNLDLVIPS
                     FIDLATPPWFVVLFLITLLAASMSTLSSLFHVMGTAVGFDIWQHFGGRRPSLRANQVG
                     ILAMILVSVLLAFTLPGSIVARTTAMFFGLCASALLPSFAYALYSEAPSARAARASLV
                     TGAAAWFLWTAFVHRAESAPLGIARLLTGSEAILGLPWQTLDPLMIALPLSVLALGAV
                     AVVERRRGPATPVPGGKPTWR"
     gene            complement(8314..9177)
                     /locus_tag="VKO45_00255"
     CDS             complement(8314..9177)
                     /locus_tag="VKO45_00255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HMA04343.1"
                     /translation="MKPGLGVNRIHRIDCLEGLGRLGERTVDVIVTSPPYNIGIRYRS
                     YHDRRSREEYLGWMGEVSRLARRVMSEQGSFFLNVGGTVTDPWIPLDVAAAFREHLVL
                     QNLIHWVKSIALPRASMGRYPHISGDIAVGHYKPINSRRFHHDCHEFIFHFTKEGEVE
                     LDKLAIGVPYQDKSNVTRWKGNQGDLRDRGNTWFIPYETIRTARPHPSTFPVGLPEMC
                     IRDHGVERTRLVLDPFMGIGSTAVACVRLGVPYLGFEIDPEYIAIAEERVGAEGREKD
                     SGGGDGNSRHP"
     gene            complement(9255..10361)
                     /locus_tag="VKO45_00260"
     CDS             complement(9255..10361)
                     /locus_tag="VKO45_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HMA04344.1"
                     /translation="MAAEVFYTGLRARKEGDSRIAKIRRLFSAAGFGDCIRERDFCAI
                     KLHFGEQGCDTHVSPVHVRQVVDLIRAEGGRPFLTDTGTLYSGSRSNAVDHIATAISH
                     GFDYAVVGAPVIIADGLRSGHVAEVKIGKKHFNRVFIAGDIARADSMMVISHFTGHDT
                     AGFGGAIKNLAMGCASARGKREMHEAHAMTVPERCRGCGSCVEVCPAGALSLKGEKVS
                     LDRETCLGCGECLTACLQSAIEFDWTVDIPAFTERLVEYACGALEGKRDRAGFFNFLL
                     SVTPDCDCVRWSDAPLVSDIGILASRDPVAIDAASLDLVNRQAGLAGTLLETHHRPGE
                     DKFRGVWEHTRGDLQISYAEECGLGTRDYRLREI"
     gene            complement(10602..10778)
                     /locus_tag="VKO45_00265"
     CDS             complement(10602..10778)
                     /locus_tag="VKO45_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04345.1"
                     /translation="MIGIEKYIDFLDRQGLQVLKGNELEIHPFTSHGACRRVPAQIRG
                     KNGMDKTVRLIKRL"
BASE COUNT         2034 a         3516 c         3524 g         1903 t
ORIGIN      
        1 aggggatcac caccaccctc ggccggagcg gctccgacta ctccgcgacg gtcgtggggg
       61 ccgccatcgg tgccgacgag gtctggatct ggacggacgt agacggcatc atgaccaccg
      121 acccccggct cgtccgggag gcccgggtcc tccccacggt ctcgtacctc gaggccatgg
      181 agctctcgta cttcggcgcc aaggtaatgc acccccggtc catcgagccg gccatgcgaa
      241 aggagatccc catccgggtg aagaacacct tcaacccctc ccaccccggg acctgcatca
      301 cgaaggagaa gcggaagtcc cgccgggtgg tgaaggccct cacccacatc accggggtcg
      361 ccctcgtgaa cgtgaacggc gcccagatga tcggccggcc gggcgtcgcc cgggccatct
      421 tctccgccct cgccgaccgg gaggtgaacg tcatgatgat ctcccaggga tcctcggagg
      481 cgaacatctc catcatcgtc gagctcgacc acatggagat cgcggcggag gccctgaaac
      541 cgatggtgga gcagggtatc gtgcgcgagg tgacggcgaa ccccgatgtt gccgccgtcg
      601 cggtgatcgg ggccgggatg atcggcaccc cgggcatctc gggcaggatc ttcaccgccc
      661 tcggcctcgc aggcgtgaac atcatgatga tctcccaggg atcctccgag gtgaacatct
      721 ccttcgtcgt gaaggagtcc gacggcccga aggcggttcg ggtgctccac gaggagctcc
      781 gcctctcgga ggagcaggat gggtagggag acctaccgcg ccgccggtgt cgacatcgac
      841 ctcgtgaagg acgccatcgg ggccctcacc gggaaggtga ccttccgccg gaagaaaccg
      901 tacgggatgc tcggcggcgt cggtcattac gccgggctca tcgacttcgg agagcatgtc
      961 ctcgccctga ccaccgacgg cgtcgggacc aagatgctcg tggccgatca gctccgggac
     1021 tggcgcaccg tcggcatcga ctgcatcgcc atgaacgcga acgacctctt cgtcatgaac
     1081 gtggagccgg tggccttcgt ggactacatc gcgaccgacc ggctctcggt ggagaagatg
     1141 gcccagatcg gcgaggggct gaacgagggg gcacgccttg cgaacctgaa cctggtcggc
     1201 ggggagaccg cccagctgcc cggcctcgtg aacggcctcg accttgccgg gacctgcctc
     1261 ggggtgcagg agaaggggaa ggtggtcagc ggcgagaaga tccggcccgg ggattgcatc
     1321 ctcggggtcc cgtcgagcgg tatccactcg aacggcctca ccctcgcccg gcgggtagtg
     1381 gagaagtcgg gcggatacgg cgagcggctg cccggcggaa tgaccgtcgg gaaggccctc
     1441 ctcaccccca cccggatcta ccgcgaatcc ctggaggcat gcaaaaaggc cgaggtgcac
     1501 gggatgtgcc acgtgacggg gagcgggctc ctcaacttca cccggctcac gaagtacggc
     1561 ttcgagttca ccgaccccct cccggttccc ccggtcttct cctggctcca ggagaggggg
     1621 gagattgaac cggtggagat gtaccggacc ttcaacatgg ggatgggatt tgcctacatc
     1681 ctccctgaag aggaggctga tatcctgaga aagttcatcc ccggctcccg ggtggtgggg
     1741 gagatcgtgg agaagtcagg catccggatg aagggtctcc ccgtcaggta atgagcccgg
     1801 agattccccg ggatcctcgt ccacgcaggc gggaaccggg gtctttgtca ccctcgggga
     1861 gatgatggcc gagttcccgg tgggatcctc gagggaaagg gtgaagggga ggctcccctc
     1921 cctcgcgagt tcgatcctcc tccggaggac gagggcgttc tcccgctcct cgccctcggc
     1981 ccaggtgagg accccggaga cgacgtcgtc tatcctttcg aggaccccct ccacgttgga
     2041 gacgaagccc tcgcaggccg ggccgggatc gatctccacc ccgagctccg ggatcctcat
     2101 cgtcccggag gaggaccgca ccacccgggc gttcaggtcc tccggtccct cgatccggag
     2161 ctcccaccgg gcgggttcgc ccccggagag aaccatggtg tcggcatgcc ggtacccgca
     2221 ctcgcacctt gcgctcacga taaggatatc ggaaaaatag gggatatttt ctgtctggta
     2281 gaggtattgt atctcccgtt cacagtatgg acagcggccc gggacgacgc ttcgcactca
     2341 ggggctcccg ccgccgatct tctcccgggc gatcttcacc gacatcgggg tgatgaccac
     2401 gtagcgctgg tccccgaggc cgacgatgtc cccgttcacg tcctttgcga cgtccttcaa
     2461 gtccttgaga acccgctcgt acgtgatctt gtccatcttc agccgcccga tgtcgatgat
     2521 gacgatgttc ccattgtaga cctcctcctt caccttcggg gtgtccttca ggtccccgat
     2581 ggtcgccacc ttcacgtaca tggtggcggg ctggtcgctc atcgtcccct cgaaggaggc
     2641 gaggtccagc tccatatagt cgtccgaacc ggtgggaggg ctccggccga tgatgctgtc
     2701 gaggagttta ctcatggaag aagtattgtt ccgtgttttg ttataacctt tccctcccgt
     2761 ggaccggtgg cctctccgga aggcggaggg atgaatccgc tggcgatacg gggcccttga
     2821 gagcccgatc cggtcacagc tccagcgccc agaggaggtc cccgacgtgg tggacggtct
     2881 ttgccatctt tcccttcgct tctgccatga tggccggccc gtccaggagg gccacgacca
     2941 ccgcgagggc cttccggtgc cgctcgtcga ccacgatggc cggggaccct ttccgtacgt
     3001 cgggggtgac cgagacgatg cccggtcgca tcacgtcggc gcccttcgca acgaagggga
     3061 ccgcccccat gtccacgacg atggtccgct cggggaaggg ccggtcgatg gcccccctta
     3121 ccgtcggaaa gacccagccc tcccgcttca ggaggagggg tttcttgtcg acgaggaaga
     3181 ggctgaaggg ggcgtcgatc tccgccatct ccacccggtc cgagcggaac ttctccgccg
     3241 agggtccgat ctccctcccg aggagggtga agagctcggc gagctcgtcc ttccggatgg
     3301 tgtgcctttt tttcacggtg agggccgcca tgtcccaatg cctccgggat gagtaccggt
     3361 gggccgcatc ctcgttatat ttatctatct tccttgcgca ctatgagtac cccaagcgat
     3421 tgtccaggga tttttatgac caagagaccg atggagattc tcgatcaggc gctcaaccgg
     3481 cagccggtca tcgtctccct gaaagggaaa cgggagatcc ggggtatcct ccagggatat
     3541 gacgtccata tgaacctggt tctggaccag gccgaggaag tcggcggaga ggccaggaag
     3601 ctcgggaccc tcatcgtgag aggggacaat gtgatctata tctcgccgat cgtggggtaa
     3661 gggagagagg ttcatcgaat gtcaaaggga acgccctcga aagggaagag acagcacaag
     3721 gtacacatcg cctgcaggcg gtgcggacac atctccttcc atgtccggca caagacctgc
     3781 tcatcctgcg ggttcgggcg gtcggcccgg atccggggat atcgctggat gaacaagaag
     3841 ccgaagatcc ccacccatta gagggggcat gtgcgggatc gttggcatca tcgatgccgg
     3901 cggcgtatcc cgtcccctct attttgctct ttttgccctc cagcaccggg gccaggagag
     3961 cgccggcatc tcgacctggg acggcacctg cttctacaag cacaagggca tgggcctcgt
     4021 ctccgaggtc ttcaacgacg ctatcctctc cgagctcccg ggccaggtcg gggtcggcca
     4081 tgtccggtac cccacgaccg gcgcgaacct ccccgagaac tcccagccct ttaacttcag
     4141 gttccgcgac caccacttcg ccatcgccta caacgggaac ctggtgaact ccgccgagct
     4201 caggagcgag ttcgagcagg acggcgagat cttctccacg accaccgaca ccgagatcgt
     4261 cgccgcgacc atcgtccacg agttccggaa gtcggggaag atcgaggagg ccatcatcca
     4321 ctgcctccac gtcctccggg gctcgtactc ggtcgtcctc atgctcgacg gcatcctcta
     4381 cgccttccgg gacccgatgg ggatcaagcc cctctgcatc gggaccacca gaaacgggtt
     4441 catcgttgcc tcggagagcg tcgccatcga caccctgggc ggaaccctca tccgggacgt
     4501 ccagccgggg gagctcttcc ggattaccgg gcagggtttc gaggcgatgc agatcgcggt
     4561 cgccgaccac cgggcccact gcatcttcga gtacatctac ttcgcccggg cagactcggt
     4621 catcgagggc cggctcgtct acgacgtgag gaggaagatc ggcgcctccc tctacgacga
     4681 ggccccggtg aaggcggacc tcgtctcccc tgtccccgat tccgggacat cggtggccgt
     4741 cgggttctcc caccactcgg ggatcccgta cctcgaggcc ctcatgaaga accggtacac
     4801 cgcgaggacc ttcatcaagc catcccagaa ggagcgggag ctcgcggtgc ggctcaagct
     4861 caaccccatc atcgggcaca tcgacgggaa gaaggtcgtc ctcgtggacg atagcattgt
     4921 gagggggacc acctcccggc acatcgtgga gctcatgaag gagagcgggg cctcggaggt
     4981 ccatgtccgg atcgggtcgc cccagatcat cgccccctgt tacctcgggg tcgatatgcc
     5041 cacccgggcc gagctcatcg cgagcacccg gcccgtggac gaggtgaagg agtccatctc
     5101 ggcgacgagc ctccaccacg tctccctgga ggccctggta aaggccatcg ggatcgaccg
     5161 ggagaacctg tgcaccggct gcctcaccgg gaagtacccg ctcttcatcg agggcgagtc
     5221 ctgcctctcc cggaacatcg acttcgtcgt ggggagctac caggcgaagc tcgggtcctt
     5281 cccccaggga aggaccgccg caaaaacggc tggaaaagga aaatagcgag ggatggattt
     5341 gaaccatcga tctacgggtt atgagcccgt cgggatatcc tgactacccc acctcgcttc
     5401 ccgaaaaggc accatactat tcttgatgat gcgtgatata ctttgctccg agccgccccg
     5461 ccccggctca ccacggataa ataccgagga gccctatgtc ttgtatggac gatgagctcc
     5521 agcggatccg ggaaaagaaa ctcgaggagc tgaagcgggg catccagggg aagccggaaa
     5581 agcccgacag gcctggagca acaccggagg gacaggtcat ggtcctcgac gagcggcagt
     5641 ttgcggccgc ggtgcgggac aacccctgcc tcgtggtgga catgtgggcc ccgtggtgcg
     5701 ggccctgccg gatggtggcg ccggtcgtcc aggagctcgc cgccgagttc gcggggaaga
     5761 tccagttcgc aaaggtgaac accgacgaga accagcgggt cgccgcgacc tacgggatca
     5821 ccgccatccc cacgatcctc ctctttgccc ggggaaggct cgtggaaagg gtcatcgggg
     5881 cctacccgaa gccgatgctc cgggaccgga tcctccgagc tttcgggatg acgggttgat
     5941 ccgagggttt acccgaggat caacccgtgc gggtgagccc atcagggcta acccgctaca
     6001 gggtaatccg agcgcagcga ggattgccct gtacagggaa acccatcagg atttcccgtg
     6061 gccggttaac ccgggtggta acccgcaact gcgtgaacag tacagggcga cccgcggggt
     6121 gctagtgcgc tccctcgacc ctccaggcgt ccgagatctt tgcggcgagg atgaggtcat
     6181 tcagggtgat cccgccgctc gcgtgggtgt agcaggagat ggtgacattt ctctgctcgg
     6241 tgatgcacat ctcggggtag tgcatctcct ccgccgcgat caccgacacc cggttcagga
     6301 acttcacggc ctcgacgaag cttttgcagg cgaacctccg gttcagcctc gtcccctcca
     6361 gggcccagtc gggaacctcc ttcacgaggg agagaagctt cctccgggtg aggggcggcg
     6421 tcccgggctc gatgggggtg cacttctcct gagccagatc catgtataca catacccagc
     6481 agggagagaa aaagtttctt taccccccgt gagggagggg cacaccaaag atgggcttcg
     6541 tccggaggga ggtgagcacc gaaggtgcga actgactgga ggacgtcgag caccgaaggt
     6601 gcgaaccctc ccgtagggag aagctcaccg ccaggtgggc ttccctcccg gtacgggagt
     6661 ggctggcccc cgacgcctct cgacgacggc gaccgccccg agagccagaa cggacagcgg
     6721 gagggcgatc atcagggggt ccagggtctg ccagggtaat cccaggatgg cctcggaccc
     6781 ggtgaggagc cgggcgatgc caaggggggc ggactcggcc cggtggacga aggcggtcca
     6841 gaggaaccac gccgccgccc cggtgacgag gctcgcccgg gcggcccgcg ccgagggggc
     6901 ctccgagtac agggcgtacg caaaggaggg cagaagcgcc gaggcgcaga gcccgaagaa
     6961 catcgccgtt gtcctcgcga cgatggagcc gggaagggtg aaggcgagga ggaccgagac
     7021 gaggatcatg gcgaggatcc ccacctggtt cgccctgagg gagggcctcc ggcccccgaa
     7081 atgctgccag atgtcgaacc ccacggcggt acccatgaca tggaagaggg aggagagggt
     7141 ggacatggat gccgcgagaa gggtgatgag gaagaggacc acgaaccagg ggggggttgc
     7201 taggtcgatg aagctcggga tgaccaggtc caggtttccc cccgctaccg cggccatggc
     7261 gatctgcccg gtatcccggt agaagaagac gttcgacagg gcccctaccg tgaaggtgac
     7321 gccggtcatc ataagaatga agggcccgcc cacgagcacc gcccggtcca gggtccggga
     7381 gtccttcgcg gtcatgaacc ggaccacgag ctggggctgg gcgagggcgc cgattcccac
     7441 tccaaggatg agggtggtga ccagggtgta ccagatcggg gacccgaggg ccgggagggt
     7501 ggtccagccc gtgagaccct gcgcctggag atcggcgggg atgagggggg cgaggccggt
     7561 gagggcctcg tgggcggcag ccaccccgcc caggagggag tacgtgaaga cgagaaggat
     7621 ggccatcccg atgaacatga cgatggcgtg gagggagttc gtgtacatca ccgcgatgag
     7681 gccgccggcc acgacgtaca ccgcggtcac gagcccgagc acgatgaccg cccacccgaa
     7741 gggcatgggg atggtctttt ccaggaaccg ggcgccgccg atgaggatgg ccgaggtgta
     7801 gaggggcatc ccgaggatga tgacggccgc cgaggcgtac tgcatgaacc cgtcagagaa
     7861 gcgcctcccc atgaggtccg ggaaggtgac cgctcccagg cgcttcccga tctcccgggt
     7921 cctcttcccg aagacgagga aggcgacgag gatgcccatg ccgatgttta agaccgtgag
     7981 ccagacgagc cccatcccgt agaccgctgc cacccctccg aagccgatga tggccgaggt
     8041 ggagatgaag gtggccccgt aggagagggc gaggatccag ggccccgccc tcctcccggc
     8101 gagcatgtag tcctcggccc ccttcgtccg gcggtagccc aggtagccga cggcgagggt
     8161 gaggacgacg tagatcccgg tcaccgcgag gaaggtcccc gtgtccacgg gcattcctag
     8221 tcccccgccc cccggtgcca ggagaggagg ccgtacagga tgcagaggag ggcgaggcct
     8281 atcgcgaggg catagccgag gacgatccag ggatcaggga tgccgagaat tgccatcgcc
     8341 gcctcccgaa tccttctctc tgccctcggc accgaccctc tcctcggcga tagcgatgta
     8401 ctccgggtcg atctcgaacc cgaggtaggg gaccccgagc ctcacgcagg caacggcggt
     8461 cgacccgata cccatgaagg gatcgaggac gagccgggtc ctttccacgc catggtcccg
     8521 gatgcacatc tccgggagcc cgacgggaaa ggtgctcggg tggggccggg ccgtccggat
     8581 ggtctcgtag gggatgaacc aggtgttccc cctatcccgg agatcgcctt ggttgccctt
     8641 ccacctcgtg acattggact tgtcctggta gggaaccccg atggcgagct tgtccagctc
     8701 cacctccccc tccttcgtga agtggaagat gaactcgtgg cagtcgtggt ggaacctccg
     8761 gctgttgatg ggcttgtaat ggccaacggc gatatccccg ctgatatggg ggtacctgcc
     8821 catggacgcc cggggaaggg cgatggactt cacccagtgg atcaggttct ggaggacaag
     8881 gtgctctcgg aaggctgcag ccacatccag gggtatccag gggtcggtga cagttccccc
     8941 gacgttcagg aaaaaggagc cctgctcgct catgacccgt cgcgcgagcc gggacacctc
     9001 gcccatccac ccgaggtact cctcccggct ccggcgatca tgatacgagc ggtaccggat
     9061 cccgatgttg taaggcggcg acgtgacgat gacatcgacc gtcctctcgc ccagtctgcc
     9121 gagaccctcc aggcagtcga tgcggtggat acggttgacc ccgagaccgg gtttcacctc
     9181 accgaccccc tctccctcga aatcccttcg ggctccggct gaaatccatc ctgcacccac
     9241 ccgtgctggc gccgtcagat ctccctcagc cggtagtccc ttgtcccgag accgcactcc
     9301 tcggcatagg agatctggag atcccctctc gtgtgctccc agacccctct gaacttgtcc
     9361 tccccgggcc ggtgatgggt ctcaagaagg gtcccggcaa ggccggcctg cctgttcacg
     9421 aggtccaggc tcgcagcgtc gatggccacc gggtcccggg atgcgaggat gccgatgtcg
     9481 gaaacgaggg gggcatcgga ccagcggacg cagtcgcagt ccggggtgac ggagaggagg
     9541 aagttgaaga aaccggcccg gtcacgtttc ccctccaggg ccccgcaggc gtactcaacc
     9601 agccgctcgg tgaaggccgg gatgtccacc gtccagtcga actcgatggc ggactggagg
     9661 caggcggtca ggcattcccc gcagcccagg caggtctccc ggtccaggct caccttctcc
     9721 cccttcaggg agagggctcc cgccgggcac acctcgacgc aactcccgca tccccggcac
     9781 ctctcgggga cggtcatggc gtgggcctcg tgcatctcgc gcttgccccg cgccgatgca
     9841 caccccatgg cgaggttctt gatggcaccc ccgaaccccg cggtatcgtg ccccgtgaag
     9901 tgggagatga ccatcatgct gtcggcccgg gcgatgtccc cggcgatgaa gacccggttg
     9961 aagtgtttct tcccgatctt cacctcggcg acgtgcccgc ttcggaggcc gtccgcgata
    10021 atgacgggag cgcccacgac cgcgtagtcg aatccgtggg agatggccgt cgcgatgtgg
    10081 tccacggcat tcgaccggct tcccgagtag agagtcccgg tgtcggtaag gaagggccgg
    10141 cctccctcgg cccttatcag gtccaccacc tggcggacgt ggaccgggct cacgtgggtg
    10201 tcgcagccct gctcgccgaa gtggagcttg atggcgcaga agtccctctc gcggatgcag
    10261 tccccgaacc cggccgcgga gaagagcctc cggatcttcg cgatcctgct gtccccctcc
    10321 ttcctggccc ggagcccggt atagaacacc tcggctgcca tgggaaacct cccgcactac
    10381 accgggaatc tccccgccca taaaccctcg gtcaccaggt gagaccaccg tctatcggat
    10441 gccatggggg accccgtttc cttcgcatct cccctgtgga acggttatac ggttgtttcc
    10501 tctttttcca caggaaacac cgaaacgccc gggcttctgc catgcatacc atatagattt
    10561 tgtgctacag gaatacaact atcgggagag aattgcctga atcaaagcct tttaatgagg
    10621 cgaactgtct tatccatccc attcttcccc ctgatctggg ctggtactct ccggcaggct
    10681 ccatgggaag taaaggggtg gatttcaagc tcatttcctt ttagaacctg taagccctgc
    10741 ctgtccagaa aatcaatata tttttcgata ccgatcattc cctcctcaat gatttggtga
    10801 atgccatctt gatggcgggg tggatgtcac cggatgaaca atccggtggt tgcatgtcct
    10861 ccaatggaag taagggggtt gtttctggcg aatatttctt attcaatata taccttcctt
    10921 tttaaggaga tcgcgtacct ggccgtcctt gtttttcccg gttcaattca cgccgga
//
LOCUS       DATGDI010000008        44140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_378784, whole genome shotgun sequence.
ACCESSION   DATGDI010000008 DATGDI010000000
VERSION     DATGDI010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 44140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44140
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378784"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VKO45_00270"
     CDS             <1..430
                     /locus_tag="VKO45_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA04346.1"
                     /translation="YGIALKPQDPKATRPDLVDLLIGGQKVATLKLIPADAGPQLMQL
                     ASTNAIQVAYVGNPPVISAVDKGTPVKILMALNTEGSGVVVVTDAPASDWDSFVRWAK
                     ERSAAGNPIKMAAPGKGSIQDVMLRYALEESGLSVKEVQA"
     gene            437..1198
                     /locus_tag="VKO45_00275"
     CDS             437..1198
                     /locus_tag="VKO45_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA04347.1"
                     /translation="MKRPLGILLPLLLLVVWEIVAQAIGNQFILPKLETVIPVLLAPT
                     VDILGTGSLLHNALVSLERVGAGFLVALAIAIPLGVGMGWSGRMDALVDTTIQLLRPI
                     PPLAWGPLAIAWFSVGLNSMVFIIFLGAFFPILLNTIDGVRGVKKTWVEVAQTLGASE
                     RQVLAKVVLPGAAPTIWTGLRVGFGIAWMCVIAAEWLPGTTSGLGYLTLYAYNFGQMH
                     YIIAGMISIGIIGIAFDLLFRYVEDRRFAWRGLER"
     gene            1195..1953
                     /locus_tag="VKO45_00280"
     CDS             1195..1953
                     /locus_tag="VKO45_00280"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA04348.1"
                     /translation="MKLAIQDLSKVFVTGTGERVPALEKIDLTIEDEEFLCILGPSGC
                     GKTTLLRIIAGLDTPSSGSILVDGEALSGPSRRIAMIFQEYSLFPWRTVLENVGFGLE
                     VLGRNGGERLATARKYLELVGLAPFEKNFPYELSGGMRQRVAVARALATEPAVLLMDE
                     PFGALDAQTRNLMQTELQEIWKKTRKTILFVTHSVDEAVFLADRIVVLTQRPGRVREI
                     ISVRKCPHPRDRTCRDFVEIRRHVLDLIAAGSSD"
     gene            2024..2830
                     /locus_tag="VKO45_00285"
     CDS             2024..2830
                     /locus_tag="VKO45_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HMA04349.1"
                     /translation="MVRKPGRMYRNLAKKAYTRREYMGGVPGSRLVQFDMGNLTEDFP
                     LEAALIVEEGCQIRHTALEAARVVINRRLQDLGRANFRLKIRVYPHHILRENKQATGA
                     GADRVSEGMRLSFGKPVGSAARVYPGQKILSVYTLLPNLEKVKDALRHGAHKLPSPSR
                     IVIATHKVSGIRATHVKIAEEKVVAPPVAAVAEAEAAAPGAEKEAEKGIEKGVEKGAG
                     KPGEKAPEKGAGKGEKTPEKGAGKPGEKGAEKAPEKGAGKGEKHHEKSRG"
     gene            2923..3765
                     /locus_tag="VKO45_00290"
     CDS             2923..3765
                     /locus_tag="VKO45_00290"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HMA04350.1"
                     /translation="MHRPDDPALLAAVAKATAEALAEAEVRLPEDVERAIRAAHEREK
                     NPVARGELGNILANIALAREGQTLICQDTGIPIIYLTIPPNLSYTRALEEAVAQGVRM
                     ATEKIPLRPNAVDPCTRRNSGDNTGPGIPAVHVRPGESLTVTVLPKGAGSENVSRLGM
                     LLPTRTADVERFVVETVALAGGRPCPPVILGVGIGSTFDGVAALAKEALLLPIDAMDP
                     FERELCDAVNSLGIGPMGLGGDTTALAVKVRKAASHTASLPVAVNVQCWAARRVTRVV
                     EGWR"
     gene            3762..4322
                     /locus_tag="VKO45_00295"
     CDS             3762..4322
                     /locus_tag="VKO45_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HMA04351.1"
                     /translation="MIGLTTPLGEEILSLRAGDRVTLSGTVYTARDEAHLRMQREGIP
                     FDPRGAVVYHCGPVVAGQRIVAAGPTTSARMDDLTGFLLDAGVRGIIGKGGMGRRVAD
                     LLRGRGVYFAFTGGCAALAASRMRLQGVRFPDLGMAEAVWVIGIDRLPLVVGIDSHGN
                     DLFERVREEAKAQCQARIHYSQGRTP"
     gene            4319..4591
                     /locus_tag="VKO45_00300"
     CDS             4319..4591
                     /locus_tag="VKO45_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HMA04352.1"
                     /translation="MKLVIDSDRCKGCNLCMLVCPYRIFKEGKEQNERGIILPELDRP
                     ERCPNCRLQKLYGRTVCGLCQMICPDQAIRWENESGNEPQKVVIEF"
     gene            4588..5688
                     /locus_tag="VKO45_00305"
     CDS             4588..5688
                     /locus_tag="VKO45_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HMA04353.1"
                     /translation="MSRVEFMQGNTACAEGALAAGCRFFAGYPITPSTEVAEHMALKL
                     PKAKGVFVQMEDEIASIAAAIGASWTGARAMTATSGPGFSLMMENIGYAAMTETPCVV
                     VNIQRGGPSTGQPTLGAQGDMLQCRFGSHGDYGIIALSPASVQEMFELTAKAFNLSDR
                     YRVPAFLMADEIVGHMRERLTIPDSVEVTGRKKLAPGTLPFQAGEDDIPGFPTFGEGH
                     GVHVTGLTHDERGYPAATNPRAHERLVTRLVRKVESARREMADYRVTNPDAEKVFISY
                     GAPTRTVHQVLHDRPKEGIGHLQLRVVWPFPEFALAEFPEAKAFLVPEMNLGQVAREI
                     ERHTDVPVIPVPKIGGDLHTPAELGKALEGTP"
     gene            5685..6473
                     /locus_tag="VKO45_00310"
     CDS             5685..6473
                     /locus_tag="VKO45_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMA04354.1"
                     /translation="MSFRDWYRVDRLPTIYCVGCGNGTVINCTLHAVDTLGWKWEETV
                     FVSGIGCSSRAPGYIRTDSLHTTHGRALAFATGIKLAKPGLHVVVFTGDGDLAAIGGN
                     HFIHACRRNIDITVICMNNWIYGMTGGQGSPTTPRGAITTTTPYGSGEGVFDLSALAV
                     AAGANHVARWTSYHVKELTRAIQTGLETPGFSLVEVLTQCPTAYGRRNKLREVSDTVE
                     YLKDHAILLQKARRLEAEGKPVPADMFTIGEFVRRSLPAMGVPP"
     gene            6470..7006
                     /locus_tag="VKO45_00315"
     CDS             6470..7006
                     /locus_tag="VKO45_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HMA04355.1"
                     /translation="MRHEIRFSGFGGQGIILAAVILGRAAALYDGKHAVQTQVYGPEA
                     RGGASMSAVIIDDEPILYPEVSVPDIYVIMSQEGFERYGAKAPKDAVMLIDSDMVEGR
                     PACSFREIPATSEARNTLGKVIVANIVMLGAVVAATGIVSDEAIEKAVLDSVPKGTEK
                     LNLAALKRGMELVREKKK"
     gene            7080..8165
                     /locus_tag="VKO45_00320"
     CDS             7080..8165
                     /locus_tag="VKO45_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HMA04356.1"
                     /translation="MRLAEHEAKEILRAYGIPVPSGVVIRSPDELGPRLGDFGDQMVL
                     KAQVPVGGRGKAGGIVIAGKDEAVSRAAALFGKEIRGIPVKEILAEERLEIGKEYYLS
                     VAVDRSSRMPLVLFAESGGVEIEAVARERPEAVRRITVSPLLPEIPPFALRDLLGGMA
                     KELSPVANRLYRIFREKDAILAEINPLATTPKGICAADAKIIVDESALARQKLDKGRA
                     QTGREREAEVHGFSYVDLEGDIGVIGNGAGLTMATIDLIALYGGRAANFLDVGGGADR
                     ERVKHAVQLVAGLPSVKVIVLNILGGITRCDEVAKGILEAGISQKVMVRLAGVNEDEG
                     RALLAGKGYTMFGTMDEAVKEAVGTTR"
     gene            8236..9102
                     /gene="sucD"
                     /locus_tag="VKO45_00325"
     CDS             8236..9102
                     /gene="sucD"
                     /locus_tag="VKO45_00325"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HMA04357.1"
                     /translation="MIYADRTTGVIVTGATGNQGRFHIPLMHAYAREVGGRGVVAGVT
                     PGKGGQEVQGVPVYNSIRDALGEHDARAAVVFVPAGGAMDSIMECAYAGIELIVAITE
                     QIPVHDTMKAIEYARLQGAVVIGPNCPGLICPGECKLGIIPAQLATRGNVGVVSRSGT
                     LTYEVIHELTRAGIGQSAVVGIGGDPVIGQTFSDVLERFEDDPETKAVVLIGEVGGSL
                     EEEGAGTTDLPLVAYIAGVTAPPEKRMGHAGAIIEGGEGDARSKIRRLDAMGIAVAER
                     PGEIPDLIRGYL"
     gene            9106..9918
                     /locus_tag="VKO45_00330"
     CDS             9106..9918
                     /locus_tag="VKO45_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HMA04358.1"
                     /translation="MANEDLMMEAAAKIAGAIGAKAIVSYTKRCECPTDIPIIWVREL
                     QIDVVQDLTMPDILEISERHMLDTAIQLYLERRLEKGQVVGVFPYAILLYDIEEGKNF
                     IRIREFEDIVPSEVMFSVLSLAMQIAVEGREGRAIGTAFIIGDGETILRHSHQAILNP
                     YEGQHQGVCDVKNRENWESIKELAQLDGVFMVNTEGFILASGRYLDASARDVVLPGGL
                     GGRHRATAAITREHPVVGVTISESGGTIRVFREGQIRISLRPYERRYPVGNG"
     gene            9972..11174
                     /locus_tag="VKO45_00335"
     CDS             9972..11174
                     /locus_tag="VKO45_00335"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HMA04359.1"
                     /translation="MSRNIRVEALDQTPIEQQRIELVERKGIGHPDSLMDGIAESVSR
                     ALSRAYLEECGAVLHHNTDQGEVVGGETLPAFGGGKVIKPIYVLLVGRATKQFNGMHF
                     PTDSIAVKAARDYIRETLTNADLDRDVIVDCRLGEGSTDLREVFRPCQDTKPRANDTS
                     FGVGHAPFSEAETIVRSLSDAIDGSLRRKYPVLGQDVKIMALRDGDIIKLTVACAMVG
                     RYCSGIHEYLEIKETMQKELEKEARRHTNRQVVVEVNTADNPDTGSVFLTVIGTSAEM
                     GDDGSVGRGNRCNGLITPNRPMSMEATSGKNPINHIGKIYNLLSTQVARECVEKVEGI
                     EEIYIRILSQIGNPIDQPLVASAQIIPKNGASFKAISREVESVMDSSFENITCITEKV
                     ISGELKTF"
     gene            11155..12033
                     /gene="hisG"
                     /locus_tag="VKO45_00340"
     CDS             11155..12033
                     /gene="hisG"
                     /locus_tag="VKO45_00340"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HMA04360.1"
                     /translation="MSSRPSDRVRLAVPNKGRIAVPVREILEKSGLHASEGNERRLIA
                     KTRDPLVEILYARPADIPEYVATGVADLGITGLDMVAERGAEVEELLDLQIGKARLVV
                     AVPEESKVTKVGDLRGARVATEFPCLAQAFFGKRRIPIAVVRVGGACEVTPHLGIADA
                     IADLTSSGDTLRTHNLREVAEILATSTRLIGNKAACRDKAEKVEEIRVAIESVVRARG
                     QCYLMMNVRRPALGKVKKVLPGLSGPTVMEVASSEDLVAVHAVVEEERIYRLIPLLKK
                     AGARDILVVPIDRMVR"
     gene            12041..12751
                     /gene="hisA"
                     /locus_tag="VKO45_00345"
     CDS             12041..12751
                     /gene="hisA"
                     /locus_tag="VKO45_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HMA04361.1"
                     /translation="MTVFPAVDILGGRCVQLVQGRRETAKAFGDPLDCARRWVRAGAE
                     ALHVINLDGAFGESGANAGKIREILRETGVFTQLGGGIRSLEDARGWLDTGVDRVILG
                     TLAVRDPETVRTLSGEAGPSRVMASVDSRGGEVVVEGWTAPRGDYLEWARRFEDLGAG
                     ALLFTNVDVEGLEEGTRREPVERLLAATRLPVVVAGGITSLADLAALKGLGAAGVVLG
                     SALYSGKIDLGKAMEACR"
     gene            12748..13329
                     /gene="hisB"
                     /locus_tag="VKO45_00350"
     CDS             12748..13329
                     /gene="hisB"
                     /locus_tag="VKO45_00350"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HMA04362.1"
                     /translation="MRKGKIERKTGETKITVGITLEGTGKAAVETGLPFLDHMLGAFA
                     RHGRFDLEVKATGDLGVDPHHLVEDTGIVLGQAIRKSVGDGKGIRRFGFASVPMDESL
                     AQVSLDCGGRSYLVFTGSFGSPSLGTIPPELFEHFFASLCTHGGLTAHITFTGRSDHH
                     KCEAVFKAFGRALGEAVATVPGEEGVPSTKGVF"
     gene            complement(13524..13730)
                     /locus_tag="VKO45_00355"
     CDS             complement(13524..13730)
                     /locus_tag="VKO45_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HMA04363.1"
                     /translation="MADLPIAAVVRIAKKNGAERVGSDAAAALVAKAEAYIANLTKEA
                     NRLAQHAGRKTIKEEDVDLAAKTS"
     gene            complement(13921..14664)
                     /gene="mtxX"
                     /locus_tag="VKO45_00360"
     CDS             complement(13921..14664)
                     /gene="mtxX"
                     /locus_tag="VKO45_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker protein Mmp4/MtxX"
                     /protein_id="HMA04364.1"
                     /translation="MRIGIGAGRDIGKVLASAKRVKAGIQVLCYTSRESAGEIRDPSL
                     ILSDRPGEALVKALMEGEIDAAVRGSLPSNDTLRSLREATGVDHLLRIALLSTRKGRM
                     FLLAPVGVDEGWTVGEKVELVERGRALAARFGLPPEVAVLSGGRLGDVGRHPAVDRTI
                     ADAEMVARLTGAVHREILIEDAIRTAGMILAPDGISGNLIFRTLVHLGEGKAHGAPVV
                     NIDRIFVDSSRASPDYSNALITASKLAKR"
     gene            complement(14661..15674)
                     /locus_tag="VKO45_00365"
     CDS             complement(14661..15674)
                     /locus_tag="VKO45_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 2 protein"
                     /protein_id="HMA04365.1"
                     /translation="MVEKSCSLDSVKKAVREYEGVTRKHAIGEMIKALRIDAPDVIAS
                     FGEDAAVIRHDDQALLLAADGIWSKLMEADPHWAGYCSVLVNIHDIAAMGGRPIALVD
                     VLSLSDEGIREHVTAGMLAACEQFNVPIVGGHLHPDTPYSVIDVAILGIARLDSVIYS
                     HTANSGDEVIAAIDLDGRVHPSCALNWDSVTMKSPERVRAQIRLLQELGETHLVTAGK
                     DISNPGVIGTLGMLLEMSEKGAVIELDAIPRPDLKAHGIGFEQWVRMYPGMGFVLTAK
                     KRHVDEVCRRFASVGMTARSIGHLNGDRNLCIEHGGEQASVFDLKRESVMGIVTNPCE
                     LNL"
     gene            15750..17099
                     /locus_tag="VKO45_00370"
     CDS             15750..17099
                     /locus_tag="VKO45_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C large subunit"
                     /protein_id="HMA04366.1"
                     /translation="MDWAEKYRPRTLGDLVGNGPAVKAMYEWARSWSPGRKPLLLHGK
                     PGTGKTSSAHALARDMGWEILELNASDQRTKDVIERVAGVAGVTASLSGAERRLIVFD
                     EADNLHGTADRGGAREILEVIRTSRQPILLIANDLYAIPSEIRTRCEPVLFRAVQARS
                     IVPRLKFICASEGIACSEAALVRIAEDGGGDLRSSINMLQGASEGRNRIGEGDVVSSP
                     KDERVSIFDLILSLFKGREDTLLAAARATDEPPDTVLQWIEANLVHLPDAGSLALAYR
                     WVARADEYLGLTFRRQYYSLWRYANALMVLGSSLAAGGRGVRARILPPARWRRISGYR
                     RQRAIRESLIRKIGDRAHIPPEVLREDQLTLLSLLVEGNPVAHTRDLSLNADELTLLF
                     HDRARAQEVVLAAEKQEKDDGEAKEAMARKESKKEGEAKPRGKDPGRKGSGGQSSLF"
     gene            complement(17140..17553)
                     /locus_tag="VKO45_00375"
     CDS             complement(17140..17553)
                     /locus_tag="VKO45_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0146 family protein"
                     /protein_id="HMA04367.1"
                     /translation="MGPYKRIERLIGDYIRGNYHCVVEVGVGQNPVSALRIRDAGIPI
                     RCTDIRPMPFPGGPEVVRDDIFTPDMSLYTGADIIYSIRPGVEMIPPLIALAERVNAD
                     LIVYHLGNEIYGDGGEIIDCGVILHRYRSRRMGEK"
     gene            17571..18116
                     /locus_tag="VKO45_00380"
     CDS             17571..18116
                     /locus_tag="VKO45_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HMA04368.1"
                     /translation="MRIKIFWDQDAPRGLESGVEKRIRSILGSDLAVEESCILLSGYD
                     RGRDQYDAQKILSRIQLFKRQRGCTEPILLVVPHDLSVRGQDFVFGLARQSVGAAVVS
                     TARLDNGYYGRTREDSDLTGRIAKEGAHEIGHLFGLEHCADPECVMFPPRTLGELDRK
                     KLAFCPACGKKFRMSEGVDSL"
     gene            complement(18194..18790)
                     /locus_tag="VKO45_00385"
     CDS             complement(18194..18790)
                     /locus_tag="VKO45_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HMA04369.1"
                     /translation="MLSLLDSALFTWIVLPVLIFVARICDVSLGTIRVIFVSRGYRHL
                     APLLGFFEISIWLFAIGQIMRDINDISIYIAYAAGFATGTYVGITLEEKLSLGTVLVR
                     VITGRDSSTLVDALKAADYGLTIHAAEGAEGPVTIVFTVVKRQELPRIIGIIKEFNPH
                     AFYSVEDVRHASEGVFPAREGGVLRSYMDPRRFFAKKK"
     gene            complement(18875..19465)
                     /locus_tag="VKO45_00390"
     CDS             complement(18875..19465)
                     /locus_tag="VKO45_00390"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMA04370.1"
                     /translation="MADPDPPGGEAETAQPDVPEEEKVDEVPYLIDLLVHPELNVRWR
                     AAEALGEARDPRAVGPLIAALRDPFQDVSWMAAEALGKIRDPAAVEPLIESLKTGEKW
                     LRQGSAWALGKIGDPRAIGPLTAALKDPKADVRKNAAWALGQFAGEQVIAALSEAIQD
                     PNPAVQRAAQQALETVKKKGEGKPPEKPPEKPPEKA"
     gene            19634..20044
                     /locus_tag="VKO45_00395"
     CDS             19634..20044
                     /locus_tag="VKO45_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04371.1"
                     /translation="MHRAVARLKVELIILFGFAIGLLILTGFFMDPSYYYPDNVFWPL
                     TAFIENLLPDIYIRVTAGLLLLLLLGLSVKRVYAIGGPGAIIGAVMGFLAGLATPSDL
                     LVGLVMLGIGLACGYIAVTGLRTLKGSWVVRPRG"
     gene            complement(20041..20889)
                     /gene="nadC"
                     /locus_tag="VKO45_00400"
     CDS             complement(20041..20889)
                     /gene="nadC"
                     /locus_tag="VKO45_00400"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HMA04372.1"
                     /translation="MPSSLPLADLLRFVEEDAPTGDLTSSALAPLGRCRAEIRAKATG
                     VIAGIEEVSALFSHFEVTVTTGIRDGDPCTPGSLVLSLEGEADRILLVERTALNILSR
                     MSGIATATRAMVEAARRVRPSLRIAATRKTCPGMRVLDKKAVVLGGGEAHRATLSDAI
                     LIKDNHLRLLPLGEAVRRARGRAPSGRIEVEVTSREEAVLAAEAGAGVIMFDNMDPPS
                     IGDAIRELEARGLRRRVLLEASGGIGPGNLEEYASLDLDRISSGSLTHSARALDLSLE
                     VVSRTG"
     gene            complement(20891..21649)
                     /gene="nadX"
                     /locus_tag="VKO45_00405"
     CDS             complement(20891..21649)
                     /gene="nadX"
                     /locus_tag="VKO45_00405"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HMA04373.1"
                     /translation="MIRVGLLGCGNVGRIIARERVGVEIAAVHDRIPERAEEVAGLCG
                     AVAYRDFADFVRADLDVIVEAASVAAVREHAAEILSHGKDLVILSVGALADRPFRDRL
                     VALAGEKKRRIHIPSGAILGLDNLKVGQISPLKRLLLRTTKSPDSLNIRAEKRMMIFS
                     GKANDCIREFPKNINVAVALGLAAGREADVEIWIDPSVDRNIHEIEAEGDFGEIFIRV
                     SNVPSPDNPATSYLAALSILTLLRNLDGPLVVGT"
     gene            21830..22411
                     /locus_tag="VKO45_00410"
     CDS             21830..22411
                     /locus_tag="VKO45_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HMA04374.1"
                     /translation="MKGESMETLYVKDREEWRAWLSANHDLRDDIWLLYPKKHTGKPR
                     IPYADAVEEALCFGWIDSNAKRIDDDTFAQRFTRRKGKGSFSQANIERMRRLIRLGRM
                     TSAGLAVFPDLRLLEGEQELRIAPDILEALQEDPQVWENFQRFPAIYRRIRIAYIEGG
                     RRHGEDAFRRRLRNFIRKTKANRRFGFGGVQTE"
     gene            22525..23094
                     /locus_tag="VKO45_00415"
     CDS             22525..23094
                     /locus_tag="VKO45_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04375.1"
                     /translation="MDDIEKGFDLIFEQLEELNTGKEDKARQILEGKARLLEKMAKTS
                     APVISRVGLSMVKRAKLDSKGEPYNAEYYQEKFLILGKTEPAKFRPDDVTRPIADQFC
                     VLSEDGKFYEIMYSSDPVFMDSYLNPLTPEQVLDIYGLEAMFMLYRAMRDYLETDRDI
                     VEALGKVIDFLFGAPALPSEEGKKEPAGG"
     gene            complement(23337..23795)
                     /locus_tag="VKO45_00420"
     CDS             complement(23337..23795)
                     /locus_tag="VKO45_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04376.1"
                     /translation="MDARQAGILIGGLVIAGVLFLLVDIYLAGIVLILDITLAIAFHI
                     MGETHHLPEVACFLSEDAKKVVLVNRGNDRALAIHVTLVPLDREFDLPELAADGRYEV
                     SLPGMISEAKALVSYENGAGRKFTRTIRLSATGESEEDLLKPVFPIFGWK"
     gene            complement(23823..23896)
                     /locus_tag="VKO45_00425"
     tRNA            complement(23823..23896)
                     /locus_tag="VKO45_00425"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23860..23862),aa:Ile,seq:gat)
     gene            24016..25785
                     /gene="iorA"
                     /locus_tag="VKO45_00430"
     CDS             24016..25785
                     /gene="iorA"
                     /locus_tag="VKO45_00430"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HMA04377.1"
                     /translation="MTKRYLLGNEAIAHAALEASVDFASGYPGTPSSEVIDILRVQEE
                     RPLYVEWSVNEKVAFENALAAAWCGLRALVTMKHVGMNVAADPLMTSGYTGVNGGFVI
                     MCADDPFAHSSQNEQDTRCYARFAHLPCLDPATVQEAHDMVIDAFALSEEIGLPVIFR
                     PTTRICHSRSDVTLGKAGTGTRKAEFPRDPKRFVLIPGHARPLHRALNEKQPKVTKVL
                     ADRNYNRFERRGKNAIIAGGIGVTYAEEILPKGVSLGKIGAYPVDWEALRTFIEGHRK
                     ILVIEEGEPVIEELVRQVSGGVPVAGKIDGTVPSEGELSLAPVSAILAAKGYIPKQTG
                     APPAPVPDVPMRPPIMCAGCMHRPTFFAIKKVWRDAVYPSDIGCYTLGLQLGVVDTTI
                     CMGSAPSLGSGINHAGDPREVVATIGDSTFLHSGIPGLLNAVYNGAEMTLVILDNRTT
                     AMTGHQPNPVSGMTAAGETRAPVSLEAICRACGVIFVETVSPYDLASTVEVMKAAKAQ
                     KGVKVVIAKQPCVITARRAGVRRGAYEVDRDACTVCGTCIRFGCPAIEMVDDRAAINE
                     LCSGCGVCAQICPSGAIGPEGKA"
     gene            25782..26369
                     /locus_tag="VKO45_00435"
     CDS             25782..26369
                     /locus_tag="VKO45_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HMA04378.1"
                     /translation="MTGSYDLLIVGTGGQGVILASNVIGEACMIEGRGVKAAETHGMA
                     QRGGSVECHIRIDGQFGPLIPPGGADLLMAFELLEALRYRHYLKPGGALVANRLVQVP
                     TSVYMQKLEVPTEEKILGRIADLGPCVLDADKKAVEAGSPLTQNMVMVGAGSLRIPLK
                     QESLEEAIRRSVPPKTVDMNLRAFRLGREAAGECR"
     gene            26360..27109
                     /locus_tag="VKO45_00440"
     CDS             26360..27109
                     /locus_tag="VKO45_00440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HMA04379.1"
                     /translation="MPVTGRCGPFRIDRIYPGDARELLPKVPDCSVDLVVTDPPFAID
                     FRPQRNNYHRTGSRVMEGYREIAAEDYLAFTLEWMGQAFRLLRPLGSLYVFSGWNRLR
                     DVLQALDETGFTTVNHLIWKYQFGVFTRKKFVTSHYHILFAAKDPKNYFFSKAEHYPE
                     DVWVINREYWTGKRKTPTKLPLALVKKILSFSSRGGDLVLDPFLGSGTVAAAAKGMGR
                     HYLGFEIVREYLDLARERLEGTEWGSAEEGN"
     gene            complement(27091..27175)
                     /locus_tag="VKO45_00445"
     tRNA            complement(27091..27175)
                     /locus_tag="VKO45_00445"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27141..27143),aa:Ser,seq:gga)
     gene            complement(27280..28206)
                     /locus_tag="VKO45_00450"
     CDS             complement(27280..28206)
                     /locus_tag="VKO45_00450"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HMA04380.1"
                     /translation="MKETEGGDIPRSGIVVIDKPRGPSSHQVAAWAGKILGLPVGHAG
                     TLDPGVSGVLVVMLGPAVRLAPYLQRGGKEYICLWRLHGDVPGERIAEVAREFEGRIY
                     QRPPKKAAVKRALRIRTVNRLEVLELAGRLVLLRVSCDAGTYIRSLCHHMGLAAGPGG
                     SMRELRRTRSGSFAEADTHTLQELEDAAHDAREGNPVPLQGMILPVERGVADLPRLTI
                     RDTAVDAVCHGASLAGVGVMGVEGIFGSRDPLGVFTQKGELVAIAEALVGSEKLVPGS
                     TGLVARPRAVFMPPATYPKGWKKRKDRQATSG"
     gene            complement(28203..28904)
                     /locus_tag="VKO45_00455"
     CDS             complement(28203..28904)
                     /locus_tag="VKO45_00455"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HMA04381.1"
                     /translation="MQDFTMRIAVTRVEGKGARDAETCRRHGHECYTVSPLRVEIYPD
                     RVRAFADAANRGDLDCIFFASAVPAGVVSPLLTSPTRVIAIGPQTARVLANSGIRSET
                     LPSFYSKALVPYLGDWIRGKRIGIPRADVPNPALISAIEDAGGIPVEVRCYGLMATGI
                     PLATRDADALLFTSGTSFSRAVWEPRPGLLLLAIGEVTAGAMRDGGVVPAVVGDGSLE
                     GTLGALNRYLGGNRE"
     gene            28968..30182
                     /locus_tag="VKO45_00460"
     CDS             28968..30182
                     /locus_tag="VKO45_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HMA04382.1"
                     /translation="MVAGILAGCIVGLSLGTVSGLVPGVHSNTVASALLSLQGPLISV
                     LGPEGLAATLFSTLITHTFLSSIPGTFLGIPDPDTALSVLPAHALCLEGLGEEAVRIA
                     ALGSALAVVVGIPLSILCLLLLPPLQPYLDWGMGILLTGIAGILIVTAESPSWALSLF
                     TVSGFLGIFSFRYSFLAWHPFGGTTVLLPLLSGLFGFSALLLASGAPVPAQRFAGIRI
                     PGRTIAARTLLGAVAGSIVGWLPGLSNATANALVASAGGYDREPRQYILSASAANTVN
                     AFVGLAALYALARTRNGVMVALAAQELPPMSTLLIAGCLAGGVAYLLTIRLSRSAGIL
                     RGLGTRNLNLAVMGLVTVLTILLSGPFGLLILLLATAAGVVPPLINVPRLYLMGAVMV
                     PVICFSLGILSA"
     gene            complement(30244..30316)
                     /locus_tag="VKO45_00465"
     tRNA            complement(30244..30316)
                     /locus_tag="VKO45_00465"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30280..30282),aa:Gln,seq:ctg)
     gene            complement(30376..31101)
                     /locus_tag="VKO45_00470"
     CDS             complement(30376..31101)
                     /locus_tag="VKO45_00470"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HMA04383.1"
                     /translation="MRIIRAPNLGRAHELAVKTVLEKGWVLETEDGEATVECDEIGIR
                     VEEPFAAPMASPRSRFQQRFLEKYADDLIHGTPAEFEYDYHGRLFDWGEGLSSAGREL
                     HVDQVAYMIRKLATQKVSRRAIAITWIPPIDEKLDDCPCLQLVQCLVRDGVLRMKVVF
                     RSNDMLSAAGANMFALAHLEKYVADALGLPCGPYTHISLVPHIYYKRDMTDLQAFCRD
                     GADIQPVEAVCGACGRCAGRGHR"
     gene            31539..34034
                     /locus_tag="VKO45_00475"
     CDS             31539..34034
                     /locus_tag="VKO45_00475"
                     /inference="COORDINATES: protein motif:HMM:NF041431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEMAR_RS02690 family S-layer glycoprotein"
                     /protein_id="HMA04384.1"
                     /translation="MVAALVVVALLAVPAMARTPDVNTLVAGDVAFVYETNLNVPAVG
                     GGTVAVAKFGNDCPVSGAGCTFTGELARITLNPAGKLDINTAPPYFGTWFPIDATGNG
                     ALGNSFQIQHADIQLDAVLNASHIDTISGKSVTRDTNMAFKLITNYAGNYYKVGSTYL
                     AKVNIELTTPGGGKIMIFGNNAVDLRAITLNGSVEYTDTLPAGSQAAGVGNAIDLTNM
                     EAGTYTATAKWTSTMPWFQLESNSNAVTFTVLSKPITVTTNKESVVRGNTFVVTITGE
                     SKHLYYVYMKDAAVTSGYIYPQITNGQSNVFLTALKINNCAIAGCTAVANDVNTLYGT
                     VVANNATGAVVNTSADGTRAIQFQTSSQASLLTYDTKYTIKVMDSQDATKYDTVDVNV
                     AKGQVTITYEGTGTYYLGETIKLTGTNTDSNAVFLFLVGPNLATNGVNLADISQKTQD
                     TVEGSFKKVDVNTDNTWSFKWDTSALTKTLDAGTYTIYAAAQAHTKDNLTNTQYSTAS
                     VVLKKPFVTASASSNTLAQGDILYIQGTAEGKPNNVYIWIFGKNYRDCQVSATVQSDA
                     SYSYKLDRGATHDLFAGQYFAVVQHPMMNGIADILGDPTGTGCLNATGTTNPVKGTWV
                     GGAPTVRLVGLQASDAATALVDLLNSPNVDDTYTKLSFMVEAPWIRIDTISDHYVGDT
                     FTITGTTNLAEGDQILMTVVSSSFQPTEKTQTGEFSGTSGTVTVQKGDTYNTFSFDVD
                     ASTFKPDEYLVTAESIEPSQTVTATFNVLEGQPTTAVTTAPPTQATTAPVTTAPTQVA
                     TTVATTAPAQPGFGALVALVGLGAVALLVLRRR"
     gene            34261..35784
                     /locus_tag="VKO45_00480"
     CDS             34261..35784
                     /locus_tag="VKO45_00480"
                     /EC_number="2.4.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP phosphorylase"
                     /protein_id="HMA04385.1"
                     /translation="MKLTVKLMDIAAPGVLLQENDARTIGVLEGDRVRVLNEANSESV
                     SALVHTTSGFLPQGTIGVYQKANGRLGFSDGASAEVRETGRLSSIDSIKGKMDGKRLS
                     KEEMFAIVKDTVEENLSPVEMTAFVTASYIHGLDMNEVEHLTRSMVETGDRIQFSTHP
                     IVDKHSIGGVPGNKISLLVVPIIAASGLKIPKTSSRAITGAAGTADLMEVLAPVEFSS
                     TEVKRMTEKVGGTIVWGGATNIAPADDRFIMVEYPFRIDAIGQMLASVMAKKSAVGAD
                     LAVIDIPVGNGTKVPTVQDGRRLASQFMELGERLNIQVECALTYGESLVGHAIGPNLE
                     VNEALSVLEGAQVPGSLIQKSLGLAGIALEMAGKAARGQGYAVAEGILSSGKALEKLR
                     QIIEIQGGNPKVQASDLVPGEHRFVVNAPLDGYVVELNNRSLITMARAAGAPHDRGAG
                     LIIHAKKGTKVKQGEPIITIHADRSWRLDKAVEVGRQLMPVLVEGMLLGHISPVHWI"
     gene            36037..39138
                     /locus_tag="VKO45_00485"
     CDS             36037..39138
                     /locus_tag="VKO45_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMA04386.1"
                     /translation="MRLYILALLIIFLSTISAVADAQVAPDHMIISGKDWMVANGMDT
                     STITVTVYDTAGNPLPLVSVTLQGTPPWTLSATSGVTDPFGQITTVLQPTTRSGSDPI
                     TVNASVIEAGVLKSIEQVYLQNIDHSIPQTAVPTYDVNVSIGGTTSLQVKVTDAYGNP
                     VDNRNMVETVQFTTSGSTGSGFWDGTSYVKILTAPVDSGGIAHATFLVAQSGANFVNI
                     QPPSPIPSRLITITGINDGSPYAITQAVSPGGYPYPYTSTDGSKFRVLYTLLDQFGNP
                     SGNRGVVITTSVPGEQMTLTTNSYGIVAFYYQKDIAGLYTLTATAVDNSSVTASQTVE
                     FVAANPTTMLLTASPQTMPSRDVVASTTSTLFAKVIDDMGNPVKGEVVSFTVWSYKVG
                     IYDQTVNPSIENETTVTDIPRTVLTATTDTDGLAHVTFRPGAFTTNRSDSKYSYTAQG
                     TALIQATWGSVTQTIELQYMNYPFLSVGTSVEPKTVVVNNTVNISVQLRGDGWALQPK
                     PLDVVLLWDRSGSMLFNETLDTSKSPDLITSESPDDRLVMAMAAGKLFVDKMNSSDRV
                     GLVSFGDLSGTSGWAVLYHTGNSTSDSHLYSLGWRAGRDYTLDKYGNGQQYSTDDATY
                     VTSRYPGHGAWGKYYGSAMATTDLNLSYDKSTVKNIVSNMVPNGGTPLRQGLYQAVRQ
                     IISDPDILAHRRDNAIRAIVLLTDGAWNTGGDPRGGSGAVSLPGINTGSVITLAAQNN
                     ISIYTIALGSEPYKDQLASYANQTGGKAYIANSGLDLDSIYASIAGELHTQAGVGTAM
                     DLSFHTIDVNGAMIPGIDVLKYVPKNPYSTHEFSYNATMPVIVNRWYDQTTDWDTDQN
                     LSFNVGTIFIGGVWEANFSMKVLKQGNIKILDSNSSIRFNDGSSLPIPDTYITSLPNS
                     TGIGLEGIGIRVTDVRRTDTGTNPDYVDVAWNLAYTGLEPFIQEYVDIRQEGTLEWNR
                     RMAKTVDPFTTSDALTLSISSLPAGIWEVRVTAHADDAGDDAMSTQFAIVKAEGTSKI
                     KIT"
     gene            complement(39639..39730)
                     /locus_tag="VKO45_00490"
     tRNA            complement(join(39639..39673,39694..39730))
                     /locus_tag="VKO45_00490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39695..39697),aa:Arg,seq:tcg)
     gene            39844..40272
                     /locus_tag="VKO45_00495"
     CDS             39844..40272
                     /locus_tag="VKO45_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HMA04387.1"
                     /translation="MILSASEIRRRLGEPGDLVIRPYSESCQQPASYDLRVTGHQILP
                     RGSCTLIPSLEWVEIPGDLAATLRCRSSYARRGLVLGGGFVDPGFRGQLTLCLGNLSA
                     GDIVLAPSERVVQMILHQVTGGGELYGGRYQDSQGVVQAR"
     gene            40269..41240
                     /locus_tag="VKO45_00500"
     CDS             40269..41240
                     /locus_tag="VKO45_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HMA04388.1"
                     /translation="MRLIPSERKYLEILRILKEHQDPMGAKRLSEILAERGFVLTDRA
                     VQYYLRYLDEMGFTRKVGNTGRVLTREGMAETESALVGERIGFIISKLERLAFRSTFD
                     PETGTGDVAYNLSLVPEGEVPGVARSFEKVSKAGYGFSTSYRILDRDPRVPEGHVGII
                     SLCSITIDGVLQRSGIPVTMAYGGRLGFEGGVVKGFLDLIGYRGTTIDPLQLFISAGL
                     TSIREVVASGNGVALANVREIPVPALDHVTDLLREMGRWGFTPPVSIGTRAFNAPYTP
                     HHVSVVSYSGMNLVGHGVEEGYHIRTEIGAGNIQFSKMEPGVEKGNR"
     gene            41710..>44140
                     /locus_tag="VKO45_00505"
     CDS             41710..>44140
                     /locus_tag="VKO45_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMA04389.1"
                     /translation="MDENTSYAHGREVNTTRYISILTILFLLSLLVGIPVAAAVTVEV
                     VSSQKWITANGLDSAYISVSVTDGGKAVSGAAVVLSVSSPWVLRDTGGTTPAGGQIAT
                     VFLPTTKSGTAEITATVTVPGSPIPVIGNYSQKIDASHPMRAEKKYKITASVGSITDI
                     TVRVTDIYGNPVTSLMDPNLVTFNTTSSGDNTFVDPSSKKVKVKNLAIALNDTGYASV
                     GFAVSTHPGDNFLYITPPSPLPPSLIKIRGIADLPPASITQTVTPGGKPPTLPTSTAK
                     FIIEYELTDTYGNPSTNQYLNISTNAGESRTILSNNAGNVIISYGPKATAGRYTITAT
                     SQADPNVSVVQLVQFLAGDPTNMLLTASPQSMASLDVDPGTVSSLIAKVVDASGNPRG
                     GQTVSFSIQPPVKNGSYIQTSLPYLDRKTYTIGDEVTAITDENGLATVDFYPGAFFSN
                     TSDPRFSTMAVATATVRAKWSSVTRTMDLSYKNFPYLSVYTSVDPSTVETNRTVDVTI
                     RVKGDGYALQPKPVDVYMVLDRSGSMLTDYQDRMVVLMKAAETFATKFNYTTDQLGQF
                     SFGEKGSVDATWDNNCGKDGDSSDDAAYASKNYPGSPRSYSDYAFMDIGLSRSPANIA
                     SAIKATVPGGYTPMRYALKQSIAALINDKNKGVVKALVLFSDGDYNYFGDPLARGSVG
                     STDPTSYDDLNPDYVSFSGVSSQNMAVYAKANNIKIFTIGYGAQLSSGGRDTLQKIAT
                     TTGGKYYYASTGDDLASVFTDIAGALKDTAGVATYVALDFSKVEVTDVKGVQVGTGSS
                     VFKYERIPWKST"
BASE COUNT         8322 a        14279 c        13867 g         7672 t
ORIGIN      
        1 ctatgggatc gccctgaaac cccaggaccc gaaggcgacc aggccggacc tcgtggacct
       61 cctcatcggc ggacagaagg tcgccaccct gaagctcatc ccggccgatg ccgggcccca
      121 gctcatgcag ctcgcctcca cgaatgccat ccaggtcgcc tacgtcggca acccgcccgt
      181 catctcggcc gttgacaagg ggacacccgt gaagatcctc atggccctga acaccgaggg
      241 ctcgggggtc gtcgtggtca ccgatgcccc tgcctcggac tgggactcct ttgtccggtg
      301 ggcaaaggaa cgatcggcgg cggggaatcc cataaagatg gcggcacccg gcaagggctc
      361 catccaggac gtcatgctcc ggtacgccct tgaggagagc ggtttatccg tgaaggaggt
      421 acaggcttag agaggcatga aaagacctct cgggatcctc cttcccctcc tcctcctcgt
      481 cgtctgggag atcgtcgcgc aggccatcgg caaccagttc atcctcccga aactcgagac
      541 ggtcatcccg gtgctcctcg ccccgacggt tgacatcctc gggaccggga gcctccttca
      601 caacgccctc gtctccctcg agcgggtggg ggcgggcttc ctggtcgccc tcgccatcgc
      661 gatacctctc ggggtcggga tgggatggtc ggggcgcatg gacgccctcg tggacaccac
      721 catccagctc ctccgcccga tcccccccct cgcctggggt cccctggcca tcgcctggtt
      781 ctcggtgggc ctcaactcca tggtcttcat catcttcctc ggcgcctttt tccccatcct
      841 cctcaacacc atcgacgggg tgaggggcgt gaagaagacc tgggtggagg tcgcccagac
      901 cctgggggca tccgagaggc aggtcctcgc gaaagtggtc ctccccggcg ccgcccccac
      961 catctggacc ggtctccggg tcgggttcgg tatcgcctgg atgtgtgtca tcgccgcgga
     1021 gtggcttccc gggaccacct ccggcctcgg gtaccttacc ctctatgcct acaacttcgg
     1081 ccagatgcac tacatcatcg ccgggatgat ctccatcggg atcatcggca tcgccttcga
     1141 cctgctcttc cggtatgtcg aggaccgccg gttcgcctgg agggggctcg agcggtgaag
     1201 ctcgccatcc aggacctctc caaggtcttt gtcacgggca ccggtgaacg cgtccccgcc
     1261 ctggagaaga tcgacctgac catcgaggac gaggagttcc tctgtatcct cgggccctcc
     1321 gggtgcggga agaccaccct cctgcgcatc atcgccggcc tcgacacgcc gagctcggga
     1381 tcgatcctcg tcgacgggga ggccctctcc ggcccgtccc gccggatcgc gatgatcttc
     1441 caggagtact ccctctttcc ctggaggacg gtcctcgaga acgtgggctt cggcctcgag
     1501 gtgctcggga ggaacggggg ggaacggctc gccacggccc ggaaatacct ggaactcgtc
     1561 ggcctcgccc ccttcgagaa gaacttcccc tacgagttgt cgggcgggat gaggcagagg
     1621 gtcgcggtgg cccgggcact cgccaccgaa cccgcggtcc tcctcatgga cgagcccttc
     1681 ggggccctcg atgcccagac gaggaacctg atgcagaccg agctccagga gatctggaag
     1741 aagacccgga agaccatcct cttcgtcacc cacagcgtgg acgaggcggt tttcctcgcc
     1801 gaccggatcg tggtcctcac ccagcggccc ggccgggtac gggagatcat ctcggtgagg
     1861 aagtgccccc acccgaggga ccggacctgc cgtgacttcg tggagatccg gcgccacgtc
     1921 ctcgacctca tcgccgcggg gtcctcggat tagcaaggtt taataaacga cacggccatc
     1981 ttataaacca cggattacgc ggagctccgc gaggggattt tctatggtca ggaagccggg
     2041 caggatgtac aggaacctcg ccaagaaggc gtacacgcgg cgtgaataca tggggggcgt
     2101 ccccgggtca cggctcgtcc agttcgacat ggggaacctc accgaggact tccccctgga
     2161 ggcggccctt atcgtcgagg agggctgcca gatccggcac accgccctcg aggcggcacg
     2221 ggtggtcatc aaccggcggc tccaggacct cggccgggcg aacttccggc tgaagatccg
     2281 ggtctacccc caccacatcc tccgggagaa caaacaggcg accggggccg gggcggaccg
     2341 ggtctcggag gggatgcgcc tttccttcgg gaaacccgtc ggctcggcgg cccgggtcta
     2401 cccggggcag aagatcctct cggtctacac cctcctcccg aacctggaga aggtgaagga
     2461 cgccctccgg cacggtgccc acaagctccc ctccccgtcg cggatcgtga tcgccacgca
     2521 caaggtctcc gggatccggg cgacccatgt gaagatcgcc gaggagaagg tcgtggcgcc
     2581 cccggtcgct gctgttgccg aggcagaggc tgcggcccca ggcgcggaga aggaggccga
     2641 gaaggggatc gaaaagggag tcgagaaggg tgccggaaaa ccgggcgaga aggcaccgga
     2701 gaagggagcc gggaaggggg agaagacccc cgagaagggt gccggaaaac ccggcgagaa
     2761 gggtgccgag aaggcaccgg agaagggagc cgggaagggc gagaagcatc acgagaagtc
     2821 ccggggctaa acacagccgg cccccaaccg gccctttttc tttcagatcg ttttcccttt
     2881 tcccatccac atttataaag ccgggatccc tatccctctg taatgcaccg tcccgatgac
     2941 ccggcccttc tcgcggctgt tgcaaaggcg accgcggagg ccctcgccga ggccgaggtt
     3001 cggctcccgg aggacgtgga acgggccatc agggcggctc atgagcggga aaaaaacccg
     3061 gtcgccaggg gggagctcgg gaatatcctc gccaacatcg ccctcgcccg ggaaggacag
     3121 accctcatct gccaggacac cggcatcccc atcatctacc tgactattcc cccgaatctg
     3181 tcctataccc gggcgctgga ggaggccgtc gcccaggggg tgcggatggc cacggaaaag
     3241 atccccctca ggccgaacgc ggtggacccc tgcacccgga ggaacagcgg ggataatacc
     3301 gggccaggga tcccggccgt ccatgtccgg ccgggggaga gcctgaccgt cacggttctc
     3361 cccaaggggg ccgggtccga gaacgtctcg aggctgggca tgctcctccc cacccgcacg
     3421 gcagatgtcg agaggttcgt ggtcgagacg gttgccctcg ccgggggaag gccctgtcca
     3481 ccggtgatcc tcggggtggg gatcgggtcc accttcgacg gggtcgcggc ccttgcgaag
     3541 gaggccctcc tcctccccat cgatgccatg gatcccttcg agagggagct ctgcgatgcg
     3601 gtgaactctc tcgggatcgg accgatgggc ctgggcggcg acaccacggc cctcgccgtg
     3661 aaggtcagga aggccgccag ccacaccgcc tccctccccg tcgcggtgaa tgtccagtgc
     3721 tgggcggccc ggcgggtgac ccgggtcgtg gagggctgga ggtgatcgga ctcaccaccc
     3781 cgctcggcga ggagatcctc tcgctccggg ccggtgaccg cgtcaccctc tcgggaacgg
     3841 tctacacggc gagggacgag gcacacctcc ggatgcagag ggagggtatt cccttcgatc
     3901 cccggggtgc cgtcgtctac cactgtggtc cggtggtggc ggggcagagg atcgtcgccg
     3961 ccgggcccac cacctcggcg aggatggacg acctgacggg gttcctcctc gatgccggcg
     4021 tccgggggat catcggcaag ggggggatgg ggaggagggt cgcggatctc ctccggggga
     4081 ggggggtgta ctttgccttc accggcgggt gtgcggccct cgcggcgtcc aggatgcggc
     4141 tccagggggt ccgtttcccg gacctgggta tggccgaggc ggtctgggtg atcgggatcg
     4201 atcgcctgcc cctcgtggtg gggatcgatt cccacgggaa cgaccttttc gaacgggtcc
     4261 gggaggaagc gaaagcacaa tgtcaggcgc ggatccatta ctctcaagga cgcacaccat
     4321 gaagctcgtc atcgacagcg accggtgcaa ggggtgcaac ctctgcatgc tcgtctgccc
     4381 ctaccggatc ttcaaggaag ggaaggagca gaacgagcgg ggcatcatcc tccccgagct
     4441 cgaccgtccc gagcgctgcc ccaactgccg gctccagaaa ctctacggca ggaccgtctg
     4501 cggactctgc cagatgatct gccccgatca ggccatccgg tgggagaacg agagcgggaa
     4561 cgagccccag aaggtggtga tcgagttttg agccgtgtcg aattcatgca ggggaacacc
     4621 gcctgcgccg agggggccct cgccgcaggc tgccggttct tcgcaggcta ccccattacc
     4681 ccctccaccg aggtcgccga gcacatggcc ctgaaactcc cgaaggcaaa gggggtcttt
     4741 gtccagatgg aggacgagat cgcctccatc gcggcggcca tcggggcctc ctggaccggg
     4801 gcccgggcca tgacggccac ctcggggccg ggcttctccc tcatgatgga gaacatcggc
     4861 tacgccgcga tgaccgagac cccctgcgtc gtcgtgaaca tccagcgggg cgggccctcc
     4921 accggtcagc ccaccctcgg agcccagggg gacatgctcc agtgccggtt cgggtcccac
     4981 ggcgactacg gcatcatcgc cctctccccg gcctcggtcc aggagatgtt cgagctcacg
     5041 gcgaaggcct tcaacctctc ggaccggtat cgggtccctg ccttcctcat ggccgacgag
     5101 atcgtcggcc acatgcggga gaggctgacc atccccgatt ccgtggaggt caccggccgg
     5161 aagaaactcg cccccggcac cctgcccttc caggcagggg aggatgacat cccgggcttt
     5221 ccgaccttcg gggaaggcca cggcgtccat gtcaccggcc tcacccacga cgagcggggc
     5281 tacccggcgg ctacgaaccc gagggcccac gagaggctgg tcacccgtct cgtccgcaag
     5341 gtggagtcgg cacggaggga gatggccgat taccgggtca cgaaccccga cgccgaaaag
     5401 gtcttcatct cctacggcgc gccgacccgc accgttcacc aggtgctcca cgaccggccg
     5461 aaggagggga tcggccacct ccagctccgg gtcgtctggc ccttcccgga gttcgccctc
     5521 gccgagttcc cggaggcgaa ggccttcctc gtccccgaga tgaacctggg gcaggtggcc
     5581 cgggagatcg agcggcacac cgacgttccc gtcatcccgg tccccaagat cggcggggac
     5641 ctgcataccc ccgccgagct cgggaaggcc ctggagggta ccccatgagc ttcagggact
     5701 ggtaccgggt cgaccggctg cccaccatct actgcgtcgg ctgcgggaac gggacggtca
     5761 tcaactgcac cctccatgcc gtcgataccc tcggctggaa atgggaggag acggtctttg
     5821 tctcggggat cgggtgctcc tcgcgggcgc cggggtacat ccggaccgat tccctccaca
     5881 ccacgcacgg aagggccctc gccttcgcga cggggatcaa gctcgcaaag cccgggctcc
     5941 atgtcgtggt cttcacggga gacggcgatc tcgccgccat cggggggaat cacttcatcc
     6001 acgcgtgccg gcggaatatc gacataactg tcatctgcat gaacaactgg atctacggga
     6061 tgaccggcgg ccagggctcc cccaccacgc cccggggtgc catcacgacc accaccccct
     6121 acggctccgg cgagggggtc ttcgatctgt cggccctcgc cgtggccgcg ggggcaaacc
     6181 acgtcgcccg ctggacctcc taccatgtga aagaactcac gagggccatc cagaccggcc
     6241 tcgagactcc gggcttttcc ctcgtggagg tcctcaccca gtgccccacg gcctatggcc
     6301 ggaggaacaa gctccgggag gtctcggaca ccgtggagta cctgaaggac catgccatcc
     6361 tcctccagaa ggccaggcgg ctggaggccg aaggaaagcc ggtcccggcg gacatgttca
     6421 ccatcgggga gttcgtccgg cggagcctgc cggccatggg ggtgcccccg tgaggcacga
     6481 gatccggttc tccggcttcg gcgggcaggg gatcatcctc gccgcggtca tcctcgggcg
     6541 ggcggcggcc ctctacgacg gcaagcacgc ggtgcagacc caggtctacg ggccggaggc
     6601 caggggcggg gcctccatgt ccgcggtgat catcgacgat gagcccatcc tctaccccga
     6661 ggtttccgtt ccggacatct acgtcatcat gtcccaggag ggtttcgagc ggtacggggc
     6721 aaaggccccg aaagatgcgg tgatgctcat cgactccgac atggtcgagg gccggccggc
     6781 ctgcagcttc cgtgagatcc ccgcgacctc ggaggcgagg aacaccctcg ggaaggtcat
     6841 cgtggcaaac atcgtcatgc tcggcgccgt cgtagccgca acagggatcg tctcggacga
     6901 ggccatcgag aaggcggtcc tcgacagcgt accgaagggc accgagaagc tgaatcttgc
     6961 ggcgctgaag aggggaatgg agctcgtccg ggagaaaaag aaatgaagcc aaccccgagg
     7021 atacatcagc accagagcgc gctgaagaaa ggcatggagc tcgtccgggg gaagaaggga
     7081 tgaggctcgc ggagcacgag gcaaaggaga tcctcagggc gtatggtatc cccgtaccct
     7141 ccggtgttgt tatcaggagc cccgacgagc tcgggccacg gctcggggat ttcggggacc
     7201 agatggtact gaaggcccag gttcccgtcg gtggcagggg gaaggccggt ggaatcgtca
     7261 tcgcggggaa agacgaggcc gtctcccggg ccgccgccct cttcgggaag gagatcaggg
     7321 ggattcccgt gaaggagatc ctcgccgagg agcggctgga gatcgggaag gagtactacc
     7381 tctccgtcgc agtcgaccgg tcctcccgga tgcccctcgt cctctttgcg gagtcgggcg
     7441 gggtcgagat cgaggcggtg gcgagggaac ggcccgaggc cgtacgacgg atcaccgttt
     7501 ccccgctcct tccggagatc ccgcctttcg ccctccggga cctcctcgga ggcatggcga
     7561 aggagctctc ccccgtcgca aaccggctct accgcatctt ccgggagaaa gacgccatcc
     7621 tcgccgagat caaccccctc gccacgaccc cgaaggggat ctgtgcggct gatgcgaaga
     7681 tcatcgtcga cgaaagcgcc cttgcccgcc agaaactcga caagggaagg gcccagaccg
     7741 ggcgggagcg ggaggccgag gtccacgggt tctcgtacgt cgacctcgag ggagacatcg
     7801 gggtcatcgg gaacggcgcc ggtctcacca tggcgaccat cgacctcatc gccctctacg
     7861 gcggaagggc ggcgaacttc ctcgacgtgg gcggcggggc cgaccgggag cgggtgaagc
     7921 atgccgtaca gctcgtggcc ggtctcccgt cggtgaaggt catcgtgctg aacatcctgg
     7981 gcgggatcac ccggtgcgac gaggtggcga agggaattct tgaggccggg atctcccaga
     8041 aggtgatggt ccgtctagcc ggcgtgaacg aggacgaggg gagggccctc ctcgcgggga
     8101 agggttatac catgttcggt accatggacg aggccgtgaa agaagctgtg gggacgaccc
     8161 gatgatgtac gatgaccgca ctaccaccgt cctcacatgg gcgacggccg tgaaagaggc
     8221 cgtgggggcg agtcgatgat ctacgccgac aggaccacgg gagtcatcgt cacgggagcc
     8281 accgggaacc aggggaggtt ccacattccc ctcatgcacg cctatgcccg cgaggtgggg
     8341 ggcagggggg tggtcgccgg agtcacgccg gggaagggcg gccaggaggt ccagggggtc
     8401 cccgtgtaca acagcatccg ggatgccctc ggggagcacg acgcccgggc ggccgtcgtc
     8461 tttgtccccg ccgggggggc gatggactcg atcatggagt gcgcctacgc ggggatcgag
     8521 ctcatcgtcg ccatcaccga gcagatcccg gtccatgaca cgatgaaggc catcgaatac
     8581 gcccggctgc agggggcggt ggtcatcggc cccaactgcc cggggctcat ctgccccggc
     8641 gaatgcaagc tcggcatcat cccggcccag ctcgccaccc gggggaacgt gggagtcgtc
     8701 tcccggagcg gcaccctcac ctacgaggtg atccacgagc tgacccgggc gggcatcggc
     8761 cagtccgcgg tggtcgggat cggcggggac ccggtcatcg ggcagacctt ctccgacgtc
     8821 ctggaacggt tcgaggacga cccggagacg aaggcggtgg tcctcatcgg ggaggtcggg
     8881 gggagcctgg aggaagaggg ggcgggcacc accgacctcc ccctcgtcgc gtacatcgcg
     8941 ggggtgaccg cccctcccga gaagaggatg ggccatgccg gggccatcat cgagggcggg
     9001 gagggcgatg cccggtccaa gatccgccgc ctcgatgcca tggggatcgc ggtcgcggaa
     9061 cggccgggcg agatcccgga cctcatccgg gggtacctgt aggccatggc gaacgaggat
     9121 ctcatgatgg aggccgcggc aaagatcgcc ggggccatcg gggcaaaggc catcgtctcc
     9181 tacacgaaga ggtgcgagtg cccgaccgac atccccatca tctgggtccg ggagctccag
     9241 atcgatgtag tccaggacct gaccatgccg gacatcctgg agatctccga gcggcacatg
     9301 ctcgacaccg ccatccagct ctacctggag cgccggctcg agaaggggca ggtcgtcggg
     9361 gtgttcccct acgccatcct cctctatgac atcgaggagg gaaagaactt catccggatc
     9421 cgggagttcg aggacatcgt cccgagcgag gtgatgttct cggtcctctc ccttgcgatg
     9481 cagatcgccg tggagggcag ggaggggagg gccatcggca cggccttcat catcggtgac
     9541 ggggagacca tcctccggca ttcccaccag gccatcctca acccctacga ggggcagcac
     9601 cagggagtct gtgacgtgaa gaaccgggag aactgggagt ccatcaagga gctcgcccag
     9661 ctggacgggg tctttatggt gaacaccgag ggcttcatcc tcgcctcggg ccggtacctg
     9721 gacgcatcgg cccgggacgt ggttctcccg ggcggcctcg gcggcaggca ccgggcgacc
     9781 gctgccatca cccgcgagca cccggtggtg ggggtgacaa tctccgagag cggcgggacc
     9841 atccgggtct tccgggaggg gcagatccgg atctcgctgc gcccctacga gaggagatac
     9901 ccggttggaa acggttaaat gatgacctca cccttttcta ttaagcgcgt gcagaatcac
     9961 agaggcgaag gatgagcagg aacattcgcg tggaagccct cgatcagacg cccatcgagc
    10021 agcagcggat cgagctcgtg gaacgcaagg ggatcggcca tcccgacagc cttatggacg
    10081 ggatcgccga gtcggtgagc cgtgccctca gccgggcata cctggaggag tgcggggcgg
    10141 ttctccacca caacaccgac cagggcgagg tggtcggtgg ggagaccctc ccggccttcg
    10201 gcgggggaaa ggtcatcaag cccatctacg tcctcctcgt gggtcgggcc acgaagcagt
    10261 tcaacgggat gcacttcccc accgactcca ttgcggtgaa ggcggcgagg gactacatcc
    10321 gggagaccct cacgaacgca gacctggacc gggacgtcat cgtcgattgc cggctcgggg
    10381 aggggtccac cgacctccgg gaggtcttcc ggccctgcca ggacacgaag ccccgggcga
    10441 acgacacctc cttcggcgtg ggccacgccc ccttctcgga ggccgaaacc atcgtccgga
    10501 gcctctcgga cgccatcgac ggctccctcc ggaggaagta cccggtcctc gggcaggacg
    10561 tgaagatcat ggccctccgg gacggggaca tcatcaagct caccgtcgcg tgcgctatgg
    10621 tgggacggta ctgctcgggc atccacgagt atctcgagat caaggagacg atgcagaagg
    10681 agctcgaaaa ggaggcccgg cggcacacga accggcaggt cgtggtcgag gtgaacaccg
    10741 ccgacaaccc ggacaccggc tcggtgttcc tcacggtcat cgggacctcg gcggagatgg
    10801 gggacgacgg ttccgtcggg cggggcaacc gctgcaacgg cctcatcacc ccgaaccggc
    10861 ccatgtccat ggaggcgacc tcgggcaaaa accccatcaa ccacatcggg aagatctaca
    10921 acctcctctc cacccaggtc gcccgggagt gcgtggagaa ggtggagggg atcgaggaga
    10981 tctacatccg catcctctcc cagatcggga accccatcga ccagcccctc gttgcgagtg
    11041 cccagatcat cccgaagaac ggggcgagct tcaaggccat ctcccgggag gtcgagtccg
    11101 tcatggactc ctcctttgag aacatcacct gcatcacgga gaaggtaatt tccggtgagc
    11161 tcaagacctt ctgaccgggt ccggctcgcg gtcccgaaca agggaaggat cgcggtcccc
    11221 gtcagggaga tcctcgaaaa gagcggtctc cacgcctcgg agggaaacga gcgccggctc
    11281 atcgcgaaga cccgggaccc gctcgtggag atactctatg cccggccggc cgatattccc
    11341 gagtatgtgg caaccggggt tgcggacctc gggatcacgg gcctcgacat ggtggcagag
    11401 cggggggcag aggtggagga gctcctggat cttcagatcg ggaaggcccg gctcgtcgtg
    11461 gcagtgcccg aggagtcgaa ggtgacgaag gtcggggatc tcaggggggc gagggtggcg
    11521 acagagttcc cctgcctcgc ccaggccttc ttcgggaagc ggaggatccc catcgccgtg
    11581 gtgcgggtcg gcggggcctg cgaggtgacc ccccacctgg gcatcgcgga tgccatcgcg
    11641 gatctcacct cctcggggga caccctccgg acccacaatt tgagggaggt cgccgagatc
    11701 ctggccacct cgacccggct catcgggaac aaggccgcct gccgggacaa ggcggagaag
    11761 gtggaggaga tccgggtcgc catcgagagt gtggtccggg cccgggggca gtgctacctc
    11821 atgatgaacg tgaggaggcc ggccctcggg aaggtgaaga aggtgcttcc cggcctctcc
    11881 gggccgacgg tcatggaggt tgcctcctcc gaggacctcg tcgccgtcca cgcggtcgtc
    11941 gaggaggagc ggatctaccg gctcatcccc ctcctgaaga aggccggggc acgggacatc
    12001 ctcgtggtcc ccatcgaccg gatggtacgc tgacatgggg atgactgtct ttcccgcggt
    12061 ggacatcctc ggggggaggt gtgtccagct cgtccagggg aggagggaga ccgcgaaggc
    12121 cttcggcgat cccctcgatt gtgcccggcg atgggtccgg gccggggcag aggccctcca
    12181 cgtcatcaac ctcgacgggg cctttggtga gtcgggggcg aacgccggga agatccggga
    12241 gatcctccgg gagaccggcg tcttcaccca gctcggcggg ggtatccggt ccctcgagga
    12301 cgcccggggg tggctcgata cgggggtgga tcgggtcatc ctcgggaccc tcgccgtgag
    12361 ggatccggaa acggtccgca ccctctccgg cgaggcaggc ccctcgcggg tcatggcatc
    12421 cgtggattcc cgggggggtg aggtggtggt cgaggggtgg accgcccccc gcggcgacta
    12481 cctggagtgg gcccggcggt tcgaggacct gggggccggg gccctcctgt tcacgaacgt
    12541 cgacgtggag gggctcgagg agggcacccg gcgcgaaccc gtggagagac tcctcgcggc
    12601 cacccggctc cctgtcgtgg tggctggagg gatcacgagc ctcgccgatc tcgcggccct
    12661 gaagggcctc ggggccgcgg gggtggtcct cgggtcagct ctctacagcg gaaagatcga
    12721 tctgggaaag gcgatggagg catgccgatg aggaagggaa agattgagcg gaagacgggc
    12781 gagacgaaga tcacggtggg tatcaccctc gaggggacgg ggaaggcggc ggtggagacc
    12841 ggcctcccct tcctcgacca catgctcggg gccttcgcac gccacgggag gttcgatctc
    12901 gaggtgaagg ccacagggga tctcggggtc gatccccacc acctggtgga ggacacgggc
    12961 atcgtactcg ggcaggctat ccggaagtca gtgggcgacg ggaaggggat ccggcggttc
    13021 ggctttgcct ccgtccccat ggacgagtcc cttgcccagg tttccctcga ctgcggcggg
    13081 cggagctacc tcgtcttcac cggctccttc ggttccccgt ccctcgggac catcccgccg
    13141 gagcttttcg agcacttctt tgcgagcctc tgcacccacg gcggtctcac cgcccacata
    13201 actttcacgg gaaggtcgga tcaccacaag tgcgaggcgg tcttcaaggc ctttggtcgg
    13261 gcgctcgggg aggccgtcgc cacggtcccc ggcgaagagg gcgtgccgag cacgaaggga
    13321 gtcttctagg acggaggttc caaaggaacc gacgtgagga gatgaagtct ctgaacggag
    13381 attcccgaag gaatcgacga ggggcatatc ctggaacgcc tccccttaag taaaatgcct
    13441 gatgaactgc cggatcaacc gttatgaaaa cgccgcgata gaaaagatcc tgtgtaaaaa
    13501 cgcctggact gaaaagggtt gattcaggag gtttttgccg cgagatcaac atcctcttcc
    13561 ttgatggtct tccttccggc gtgctgggcg aggcggtttg cctccttcgt caggttcgcg
    13621 atgtaggcct cggccttggc gaccagggcg gcggcagcat cgctcccgac ccgctctgcg
    13681 ccgttcttct tggcaatgcg aacgacagcg gcgattggta aatcagccat cacatttcac
    13741 ccacaaatta ctctatgagt caatatttat acttttcttt ccatttgctc aagaaatcag
    13801 gatttcttat ctcgaaggag gcaccaaatc ccccgtatcg acggatggag acgaaatacg
    13861 ggctagatga aaaacggaaa tatgggggta tttcccgaaa aaacccgttt taagggattt
    13921 tcaccttttt gccagcttag aggcggtaat cagggcattc gagtaatccg gggaagcgcg
    13981 cgagctatcc acaaagattc tgtcgatatt tactacgggg gccccgtggg ccttcccctc
    14041 cccgaggtgg accagggtgc ggaagatgag gttccccgag atgccgtccg gggcgaggat
    14101 catgccggcc gtccggatgg catcctcgat gaggatctcc cggtgaacgg ccccggtgag
    14161 ccgggcgacc atctcggcat cggcgatggt ccggtcaacg gccgggtgcc ggcccacgtc
    14221 cccgagccgg ccgccggaaa ggaccgcaac ttcggggggg agaccgaacc gggcagcgag
    14281 ggccctccct cgttccacga gctccacctt ctcgccgacg gtccaccctt catccacgcc
    14341 gaccggggcg aggaggaaca tccggccctt ccgggtggag aggagggcaa tcctcagcag
    14401 gtgatcgacc ccggtcgcct ccctgaggga tcggagggtg tcattcgaag ggagggaacc
    14461 ccggacagca gcgtcgatct cgccctccat gagggccttc acgagagcct ccccgggccg
    14521 gtcgctcaat atgagggaag gatcacgaat ttccccggca gactcccggg atgtgtagca
    14581 aaggacctgg atgccggcct tcaccctttt tgcggaggcg agaaccttcc cgatatcgcg
    14641 cccggccccg atgccgatcc tcataggttg agttcgcagg gattcgtcac gatccccatc
    14701 acgctctccc tcttcaggtc aaagacagag gcctgctccc ccccgtgctc gatgcagagg
    14761 ttccggtccc cgttcaggtg cccgatggag cgggcggtca tgcccaccga tgcaaacctc
    14821 cggcagacct cgtcgacatg cctctttttc gccgtgagca cgaagcccat cccggggtac
    14881 atccggaccc actgctcgaa accgatgcca tgggccttca ggtccggcct cgggatggca
    14941 tcgagctcga tgaccgcccc cttctcgctc atctcgagga gcatcccgag ggtaccgatg
    15001 acccccgggt tgctgatgtc cttccccgcg gtgaccaggt gggtctcgcc gagctcctgg
    15061 agcagccgga tctgggccct cacgcgttcc ggcgacttca tggtgaccga gtcccagttg
    15121 agggcgcacg aggggtggac cctcccgtcg aggtcgatgg ccgcgataac ctcgtcgccg
    15181 ctattcgccg tgtgggagta gatgaccgag tccaggcggg cgatcccgag gatggccacg
    15241 tcgatgacgc tgtacggggt gtcggggtgg aggtggccgc ccacgatggg cacattgaac
    15301 tgctcgcagg ccgcaagcat cccggccgtc acgtgttccc ggattccctc gtcggagaga
    15361 gagaggacgt ccacgagggc gatggggcgg cctcccatgg ccgcgatgtc atggatgttc
    15421 acgaggaccg agcagtagcc ggcccagtgg ggatccgcct ccatgagctt gctccagatc
    15481 ccgtcggccg cgaggaggag ggcctggtcg tcgtgccgga tcaccgcggc atcctcgccg
    15541 aaggaggcga tgacatcggg ggcatcgata cggagggcct tgatcatctc gccgatggcg
    15601 tgcttcctcg tgaccccctc gtactcgcgg accgcctttt tcaccgagtc gagggagcag
    15661 ctcttttcaa ccatccgagc gttccttcca gggtattctt tcggtcttca tgagagataa
    15721 tttatttcat ttctgaccga tgatggggta tggactgggc agagaagtac cggccccgga
    15781 ccctcggcga tctcgtcggg aacgggcccg ccgtaaaggc catgtacgag tgggcccggt
    15841 cctggagtcc gggccgtaag cccctcctcc tccacgggaa accgggaacg ggaaagacct
    15901 cctcggccca cgccctcgcc cgggacatgg gctgggagat ccttgagctg aacgcgagcg
    15961 accagcggac aaaggatgtt atcgagcggg tcgcgggcgt tgccggggtc acggcgagcc
    16021 tctcgggggc cgaaaggagg ctcatcgtct tcgacgaggc cgacaacctc cacgggaccg
    16081 ccgaccgggg cggggcccgg gagatcctcg aggtgatccg cacctcccgc cagcccatcc
    16141 tcctcatcgc gaacgacctc tacgccatcc cctccgagat ccggacgagg tgcgagccgg
    16201 ttctcttcag ggcggtccag gcacggtcca tcgtccccag gctgaagttc atctgcgcga
    16261 gcgaggggat cgcctgctcc gaggccgccc tcgtcaggat cgccgaagac gggggaggcg
    16321 acctccggtc ctccatcaac atgctccagg gtgcatcgga ggggaggaac cggatcggcg
    16381 aaggggatgt cgtgtcctcg ccgaaggacg agagggtctc catcttcgac ctcatcctct
    16441 ccctcttcaa ggggagggag gacaccctcc tcgccgcggc acgggcaacg gacgaacctc
    16501 ccgacacggt gctccagtgg atcgaggcaa acctcgtcca tctccccgat gccggatccc
    16561 tggccctcgc gtaccggtgg gtggcccgtg ccgacgaata cctgggcctc accttcaggc
    16621 ggcagtacta ctccctctgg cggtacgcaa atgccctcat ggtcctcggg tcgtccctcg
    16681 ccgccggagg ccgcggggtc agggcccgga tcctgccgcc ggcccggtgg cggcgtatct
    16741 ccggctaccg gcgccagagg gcgatccggg agtccctcat tcggaagatc ggggaccggg
    16801 cccacatccc cccggaggtc ctccgggagg accagctgac cctcctctcc ctcctcgtcg
    16861 aggggaaccc ggtcgcccac acaagggacc tctccctcaa tgccgacgag ctcaccctgc
    16921 tcttccacga ccgggcacgg gcgcaggagg ttgtcctcgc ggccgaaaag caggagaagg
    16981 acgacggcga ggcaaaggag gcgatggcga ggaaggagtc aaagaaggaa ggggaggcga
    17041 aacccagggg gaaggacccc ggaagaaagg gatcgggcgg tcagtcctcc ctcttttgaa
    17101 acccgagggg ggatctcaat cccccgaggg agaaaaaagt cacttctctc ccattcggcg
    17161 tgaacggtac cggtgcagga tcaccccgca gtcgataatc tcgccgccgt ccccgtagat
    17221 ctcgttcccc aggtggtaga cgatgaggtc cgcgtttacc ctttccgcga gagcgatgag
    17281 aggcgggatc atttccaccc ccggccggat ggagtagatg atgtcggccc cggtatacaa
    17341 gctcatatcg ggggtaaaga tgtcgtcccg cacgacctcc ggcccgcctg ggaagggcat
    17401 gggccggata tcggtgcacc ggatggggat ccccgcatcc cggatcctca gggccgaaac
    17461 ggggttctgt cccaccccca cctcaacgac gcaatggtaa tttcccctga tataatcccc
    17521 gataaggcgc tcaatacgtt tatacgggcc catcaccaca cctattgcta atgcgcatca
    17581 aaatcttttg ggatcaggat gcgccccggg ggctggaatc cggcgtagag aaacggatcc
    17641 ggtcgatcct gggcagtgac ctcgcggtcg aggagagctg catcctcctc tccggttacg
    17701 atcggggacg ggaccagtac gacgcccaga agatcctctc gcgcatccag ctcttcaaga
    17761 ggcagagagg ctgcaccgag cccatccttc tcgtcgtccc ccacgacctc tcggtccggg
    17821 ggcaggactt cgttttcggc ctcgcccggc agtcggtggg cgcggccgtg gtctccactg
    17881 cacgcctgga caacgggtat tacggccgca cgcgggagga ctccgatctc acgggacgga
    17941 tcgccaagga gggggcccat gagatcggcc atctcttcgg cctcgagcac tgcgccgatc
    18001 ccgagtgcgt gatgttcccc ccgagaactc tcggagaact cgaccggaaa aagctcgcct
    18061 tctgcccggc ctgcggaaag aaattccgga tgagcgaggg tgtcgactcc ctctgaccga
    18121 accccgggtc ccgcagggag ccggggttgt cccgggggtt gtgggcccct cgggacggag
    18181 ccggggttcc cactcacttc ttcttcgcga agaaccgccg gggatccatg tagctcctga
    18241 gaacccctcc ctcgcgggcg ggaaagaccc cctcgctcgc gtgcctgaca tcctccaccg
    18301 agtagaaggc atgggggttg aactccttga tgatcccgat gatcctgggg agttcctggc
    18361 gtttcaccac ggtgaagacg atggtcacgg ggccctcggc accctctgcg gcatggatcg
    18421 tgagaccata gtcggccgcc ttcagcgcgt cgacgagagt tgaggagtcc cgcccggtga
    18481 tgacccgcac gaggaccgtg cccagggaga gcttttcctc gagggtgatg ccgacatagg
    18541 tccccgtcgc aaacccggcg gcataggcga tgtagatgga gatatcgttg atatcccgca
    18601 tgatctgccc gatggcaaag agccagatgg agatctcgaa gaagccgagg aggggggcga
    18661 ggtgccggta gccccgggag acgaagatga cccggatggt cccgagggag acatcacaga
    18721 tccgtgcgac gaagatgagg accggaagca cgatccaggt gaaaagagcc gagtcgagaa
    18781 gcgagagcac gaaagacaga ccccgttgcc tttacaggta ctcgtttttt cgatataaga
    18841 tgatgggcac cattcggatg cccggtaaaa aaaatcaggc cttctcaggc ggtttctcag
    18901 ggggtttttc cgggggtttc ccctcccctt tcttcttcac ggtctccagg gcctgctggg
    18961 ccgccctctg caccgccggg ttcgggtcct ggatcgcctc ggagagggcg gcgatgacct
    19021 gctccccggc gaactggccg agggcccagg cggcgttctt gcgcacatca gccttcgggt
    19081 ccttgagggc ggcggtgagg ggcccgatgg cccggggatc gccgatcttc ccgagggccc
    19141 acgccgaccc ctggcggagc cacttctctc ccgtcttcag agactcgatc aggggttcga
    19201 ctgcagcggg gtcccggatc ttcccgaggg cctcggcggc catccaggag acatcctgga
    19261 acgggtccct gagggccgcg atgaggggcc cgaccgccct cgggtcccgg gcctcaccca
    19321 gggcctcggc cgcccgccac cggacgttca gctccgggtg taccaggaga tcgatgaggt
    19381 aggggacctc gtcgaccttc tcctcctcgg gtacatccgg ctgtgcggtt tctgcctctc
    19441 ctccgggcgg atcggggtcg gccatgtgcg aaaacctcgg atagtcggga gtttacggct
    19501 tcattgcact aatggtttgc ggtttctggt atcggcggga aatccgatga ggagagcggg
    19561 cccggtgaac cgaaaggatt tatgaggggc tcccgaacct atttcagggg ataccctcgg
    19621 gaagggacac gggatgcacc gggccgttgc gcgattgaag gtggagctca tcatcctttt
    19681 cggatttgcc atcggcctgc tcatcctgac gggatttttc atggatccct cctactacta
    19741 tccggacaat gtcttctggc ccctcacggc cttcatcgaa aacctccttc ctgacatcta
    19801 catccgggtg accgccggcc tcctcctcct cctcctcctc ggcctctcgg tcaaaagggt
    19861 ctatgccatc gggggacccg gcgccatcat cggggccgtc atgggtttcc ttgccgggct
    19921 cgcgacgccc tcggatctcc tcgtcggcct cgtgatgctc gggatcgggc tcgcctgcgg
    19981 ctacatcgcg gtcacgggcc ttcggaccct gaagggttcc tgggtcgtga ggccccgggg
    20041 ctagccggtc cgggagacga cctcgaggga gaggtcgagg gcacgggccg agtgcgtgag
    20101 ggaaccggaa gagatccggt cgaggtcgag ggaggcatac tcctccagat tccccgggcc
    20161 gatccccccc gaggcctcga ggaggacccg cctccggagg ccccgtgcct cgagctcccg
    20221 gatggcatcc ccgatggaag gggggtccat gttgtcgaac atgatgaccc ccgcgccggc
    20281 ctcggcggca aggaccgcct cctcgcggct cgtgacctcc acctcgatcc ggccggaggg
    20341 ggcccggccc cgtgccctcc tcaccgcttc cccgaggggg aggagtctca ggtggttgtc
    20401 cttgatgagg atcgcatccg agagggtggc ccggtgtgcc tcccctccgc ccaggaccac
    20461 ggccttcttg tcgagcaccc gcatccccgg gcaggtcttt ctcgtcgctg cgatccggag
    20521 ggacggcctc actctcctcg ccgcctcgac catggcacgg gtggccgttg cgatcccgct
    20581 catccgcgag aggatattca gggccgtccg ctccacgagg aggatccggt ccgcctcccc
    20641 ctccagggag aggacaaggg accccggggt gcagggatcc ccgtcccgga tccccgttgt
    20701 gacggtgact tcgaaatggg agaacagggc agatacttcc tcgatcccgg cgatcactcc
    20761 ggtcgccttc gcccggatct cggcccggca ccggccgagg ggggcgaggg ccgaggaggt
    20821 gaggtccccg gtcggggcgt cctcctcgac gaaccggagg agatcggcga ggggcaggga
    20881 ggagggcatt tcaggtgccc accacgaggg gcccgtccag gttccggagg agcgtgagga
    20941 tggaaagggc cgcgaggtag ctcgtggccg ggttatcggg gctcgggaca ttggagaccc
    21001 ggatgaagat ctccccgaag tccccttctg cctcgatctc gtggatgttc cggtccacgg
    21061 aggggtcgat ccagatctcg acatctgcct cccgcccggc ggcgaggccg agggccacgg
    21121 ccacgttgat gttcttcggg aactcccgga tgcagtcgtt ggccttcccc gaaaaaatca
    21181 tcatcctctt ttcggcccgg atgttcaggg agtccgggct cttcgtggtc ctgaggagga
    21241 gcctctttaa gggggagatc tggccgacct tcaggttgtc gagcccgagg atggccccgc
    21301 tcgggatgtg gatcctccgc ttcttctccc ccgcgagagc gacgagccgg tcccggaagg
    21361 gccggtcggc gagggccccg acggagagga tgacgaggtc cttcccgtgg gagaggatct
    21421 ccgcggcatg ctccctgacc gcggccaccg aggccgcctc cacgatgaca tcgaggtccg
    21481 cacggacgaa gtccgcgaag tcccggtatg ccacggcacc gcagagcccg gcaacctcct
    21541 cggccctctc ggggatccgg tcatggaccg cggcgatctc aaccccgacc cgctcccttg
    21601 cgatgatgcg ccccacgttt ccgcatccca ggaggccgac ccggatcatc agtaaccgga
    21661 tacgcttttc acggttaagc gttatgctct ccaccggtgc ttcgccccgt tggttccccc
    21721 tcgggctgat gcccatcggg ctcctcgctc ggccggcgtg gactatagcc ggcgagacgg
    21781 gttccgaggg cttaagacgc ggcaaggcga tgatcctgtt cccgggcgga tgaagggtga
    21841 aagcatggag accctctacg tgaaggatcg ggaggaatgg cgtgcctggc tctcggcaaa
    21901 tcatgacctg agggacgaca tctggcttct gtacccaaag aaacatacgg ggaaaccccg
    21961 tatcccctac gcggacgccg tcgaggaggc cctctgtttc ggctggatcg acagcaatgc
    22021 gaagcggatc gatgatgaca cctttgccca gcgtttcacc aggcggaagg gtaaggggtc
    22081 cttctcccag gcaaacatcg agcggatgag gcggctcata cggctcggga ggatgacatc
    22141 cgccggcctg gcggtcttcc ccgaccttcg gctcctggag ggggagcagg agctccggat
    22201 cgcccctgac atcctcgagg ccctccagga ggatccgcag gtctgggaga atttccagcg
    22261 gttcccggcg atctaccgga gaatcaggat cgcatacatc gaaggaggga gaaggcatgg
    22321 ggaggatgct ttccggaggc gcctgcgtaa cttcatcagg aagacgaagg cgaaccggag
    22381 gttcggcttc gggggagtgc agaccgaata acgtggtgcc gttcccgccg ttttccggac
    22441 actgactcgg ccgttccgtg agaaaggcgc agggtatatg tgccgggcaa gctctcaggg
    22501 attaaaccgg gagagaagag ccccatggac gatatcgaga agggttttga cctgatcttc
    22561 gaacagctcg aggaattgaa cacggggaag gaggacaagg caaggcagat cctcgagggg
    22621 aaggcgaggc tcctcgaaaa gatggccaaa acttcggcac ccgtgatctc ccgggtcggc
    22681 ctctccatgg tgaagcgggc caagctcgac agcaaaggag aaccgtacaa cgcggagtac
    22741 taccaggaga agttcctcat cctcgggaag accgagcccg caaagttccg gcccgatgac
    22801 gtgacaaggc ccatcgccga ccagttctgc gtcctgtccg aggacggtaa gttctacgag
    22861 atcatgtaca gctcggatcc cgtcttcatg gactcgtacc tgaaccccct taccccggag
    22921 caggtcctcg atatctacgg cctcgaggcc atgttcatgc tctaccgggc catgagggac
    22981 tacctggaga cagaccggga tatcgtcgag gcgctcggga aggtgatcga tttcctcttc
    23041 ggggcacctg ccctcccctc cgaagagggg aagaaggagc ccgccggcgg atagattgcg
    23101 ggaccgtcac ttcccacctt acggtgggaa tgacgtgttc actcgccctt ggctcgcgaa
    23161 cccgtcactt tccaccttac ggtgagaatg acggatccgt gggcacatag cccacgggcc
    23221 cgtcacttcc atccgaacat gggaaaaggg gaccgtggat gcgaacccag gggaccctca
    23281 tttcccacct tacggtgaga atgaggtgtt cactcgcctg tgggctcgcg aacccctcat
    23341 ttccatccga agatgggaaa gacgggcttt aagagatcct cctcgctctc cccggtcgcc
    23401 gagagccgga tggtccgggt gaatttccgc ccggcgccgt tctcgtagct cacgagggcc
    23461 ttggcctcgg agatcatgcc cggcagggaa acctcgtacc tcccgtcggc ggcaagctcg
    23521 ggaaggtcga actcccggtc gagggggacc agggtgacgt ggatggcgag ggcccggtca
    23581 ttgccccggt tcacgagcac gacctttttc gcgtcctcgg agaggaaaca ggccacctcg
    23641 gggaggtgat gggtctcccc catgatgtgg aaggcgatgg caagggtgat atcgaggatg
    23701 aggacgatcc ccgcgagata gatgtccacg aggaggaaga ggactcccgc gatgacgaga
    23761 ccgccgatga gaatccccgc ctgccgcgca tccattggaa atccctctct cgtattctcg
    23821 aatgggcccg atgcggttcg aacgcatgac ctcccggtta tcagccgggc gcaccacctg
    23881 gctatgctac gggcccggat agaaggtaat accctatagt ctaaagcacc cgggttttta
    23941 taggttatga atgaaaaatc agcatatcga gcccgccccg gttccctccg gggcaggaaa
    24001 ccggtgattc cctccatgac gaaacggtat ctcctgggca acgaggccat cgcccacgcg
    24061 gccctcgaag cctcggtgga cttcgcgagc ggctaccccg gcaccccgtc ctcggaggtg
    24121 atcgacatcc tccgggtcca ggaggagcgg cccctctacg tggaatggtc ggtgaacgaa
    24181 aaggtggcct tcgagaacgc cctcgcggcg gcctggtgcg gcctgcgggc cctcgtgacc
    24241 atgaagcacg tcgggatgaa cgtcgccgcc gaccccctca tgacgagcgg atacaccggg
    24301 gtgaacggcg gcttcgtcat catgtgcgcc gacgatccct tcgcccacag ctcccagaac
    24361 gagcaggaca cccgttgcta tgcccggttc gcccacctcc cctgcctcga cccggccacc
    24421 gtccaggagg cccatgacat ggtcatcgat gcctttgccc tctcggagga gatcggcctc
    24481 ccggtcatct tccggcccac gacccggatc tgccactccc ggagcgacgt aaccctcggg
    24541 aaggccggga cagggacccg caaggcggag tttccccgcg acccgaaaag gtttgttctg
    24601 atccccgggc atgcccggcc cctccacagg gccctgaacg agaagcagcc gaaggtcacg
    24661 aaggtcctcg ccgacaggaa ctacaatcgg ttcgaaagga gggggaaaaa tgccatcatc
    24721 gcgggcggca tcggggtgac ctacgctgag gagatccttc ccaagggggt ctccctcggg
    24781 aagatcggcg cctatcccgt cgactgggag gctctccgca ccttcatcga agggcaccgg
    24841 aagatcctcg tcatcgagga gggagagccc gtcatcgagg agctggtgcg ccaggtatcc
    24901 ggcggggtgc cggtcgcggg gaagatcgac ggaacggtac cgtccgaggg ggagctctcc
    24961 ctcgccccgg tctcggccat cctcgcggca aagggataca tcccgaagca gaccggagcc
    25021 ccgccggcac cggtcccgga cgtccccatg cgccccccca tcatgtgcgc cgggtgcatg
    25081 caccgcccca ccttctttgc catcaagaag gtctggcgcg acgcggtcta cccgagcgac
    25141 atcggctgct acaccctcgg gctccagctc ggggtcgtgg ataccaccat ctgcatgggg
    25201 tcggccccgt cgctcgggag cgggatcaac catgccgggg atccccggga agtggtcgca
    25261 accatcggtg attccacctt cctccactca gggatccccg ggctcctgaa cgccgtgtac
    25321 aacggggcgg agatgaccct cgtgatcctg gacaaccgga ccacggcgat gaccggccac
    25381 cagccgaacc cggtctccgg catgaccgcg gcgggggaga caagggcgcc cgtctccctg
    25441 gaggccatct gccgggcgtg cggggtcatc ttcgtggaga cggtctcccc ctatgacctg
    25501 gcttccaccg tcgaggtaat gaaggcggcg aaggcccaga agggggtaaa ggtcgtcatc
    25561 gcgaagcagc cctgcgtcat caccgcgcgg agggccggcg tccggcgggg agcctacgag
    25621 gtggaccggg acgcatgcac cgtctgcggg acctgcatcc ggttcgggtg ccctgccatc
    25681 gagatggttg acgaccgggc tgccatcaac gagctctgca gcgggtgcgg ggtctgcgcc
    25741 cagatctgcc cgtcgggggc catcggcccg gagggaaagg catgaccggc tcctacgacc
    25801 tcctcatcgt gggaacgggg gggcaggggg tgatcctcgc ctccaacgtc atcggtgagg
    25861 cctgcatgat cgagggccgc ggggtgaagg ccgccgagac ccatggcatg gcccagcgcg
    25921 ggggatcggt ggagtgccac atcaggatcg acgggcagtt cggccctctc atccctccgg
    25981 gcggggcaga cctcctcatg gccttcgagc tcctggaggc cctgcggtac cgccactacc
    26041 tgaagccggg cggggccctc gtggcaaacc ggctcgtcca ggttccgaca tccgtctata
    26101 tgcagaagct cgaggtcccg accgaggaga agatcctcgg gcgtatcgcc gacctcggtc
    26161 cctgcgttct cgatgcagac aagaaagcgg tggaggcagg gagtcccctc acccagaata
    26221 tggtcatggt gggggcgggg tccctccgga tccccctcaa gcaggagtct ctcgaggagg
    26281 ccatccgccg gtcggtcccc ccgaagaccg tcgatatgaa cctgcgtgcc ttccggctcg
    26341 ggagggaggc agcaggggaa tgccggtgac cggccgttgc ggcccgttcc gcatcgaccg
    26401 gatctacccc ggggatgcac gggagcttct gccgaaggtg cccgactgct ccgtcgacct
    26461 tgtggtgacc gaccccccct ttgccatcga cttccgcccc cagagaaaca attaccaccg
    26521 caccggctcc cgcgtgatgg agggataccg ggagatcgcc gcagaggact acctcgcctt
    26581 cactttggaa tggatgggac aggccttccg gctgcttcgc cccctcgggt ccctctacgt
    26641 cttttcgggc tggaaccggc tccgcgacgt gctccaggcc ctggacgaga ccggttttac
    26701 gacggtcaac cacctgatct ggaagtacca gttcggggtc ttcacccgaa agaagttcgt
    26761 gaccagccac taccacatcc tcttcgcggc aaaggatccg aagaactact ttttttccaa
    26821 ggccgagcac taccccgagg atgtctgggt gatcaaccgg gagtactgga cgggaaagag
    26881 gaagaccccg accaagctcc ctcttgccct cgtgaagaag atcctttctt tctccagccg
    26941 cgggggcgac ctggtcctcg acccgttcct cggctcgggg acggtggccg ccgcggcaaa
    27001 gggaatgggg agacactacc tggggttcga gatcgtcagg gagtacctcg atctcgcgag
    27061 ggagcgcctt gaagggacag aatgggggag cgctgaggag gggaattgaa cccccgaggg
    27121 gcaggacgcc ccacaagctc tccaggcttg cgccttaccg ctagactacc tcagcatgaa
    27181 atgaagattg cggaatgatc attggtcggg atctttttta aaccatccct agggggatgg
    27241 aaccatacct atggggatgg aactaccctc tcggggagat catccggatg tcgcctgcct
    27301 gtccttccgc tttttccagc ccttcgggta cgtggccggg ggcatgaaca ccgcccgggg
    27361 gcgggcgacg aggccggtgg atcccgggac cagtttctcg ctcccgacga gggcctccgc
    27421 gatggcgacg agctcaccct tctgcgtgaa gacccccagg gggtcccgtg atccaaagat
    27481 cccctcaacc cccatcaccc cgaccccggc gagggaggcg ccgtggcata cggcatccac
    27541 cgcggtgtca cggatcgtga gccggggcag gtcagcgacc cccctctcca cgggaaggat
    27601 catcccctga agggggacgg ggttcccctc cctggcgtca tgggccgcgt cctccagctc
    27661 ctggagggta tgggtgtcgg cctcggcgaa ggagcctgac cgggttctcc ggagctcgcg
    27721 catagacccg cccgggcctg cggcgagccc catgtggtga cagagggaac ggatgtacgt
    27781 ccccgcatcg cacgagaccc ggagaaggac cagccttccg gccagctcaa gcacctcgag
    27841 ccggttcacc gtccggatcc ggagcgcccg cttcaccgcc gcctttttcg ggggccgctg
    27901 gtagatccgg ccctcgaact cccgcgcaac ttccgcgatc cgctcccccg ggacatcgcc
    27961 gtggagcctc cagaggcaga tgtactcctt tccgcccctc tggaggtacg gggcgagcct
    28021 caccgctggg ccgagcatca ccacgagtac gccggagacc cccgggtcca gggtccccgc
    28081 gtgcccgacc ggtaggccga ggatcttacc ggcccaggcg gccacctggt ggctggaggg
    28141 tccccgcggc ttgtcgatga caacaatccc ggaccggggg atgtctcctc cctccgtctc
    28201 cttcactccc ggttcccccc gaggtacctg tttagtgctc cgagggtccc ctcgagggac
    28261 ccgtccccca ccaccgcggg gaccacaccg ccgtctctca tggctcctgc ggtcacctcg
    28321 ccgatggcga ggaggaggag gccgggccgg ggctcccaga cggcccggga aaaggaggtg
    28381 ccgctcgtga agaggagggc gtccgcgtcc ctggttgcga ggggaattcc ggtggccatg
    28441 agcccgtagc atcgcacctc gacggggata cccccggcat cctcgatggc cgagatgagg
    28501 gccgggttcg gaacatccgc cctcgggatc ccgatcctct tcccccggat ccagtccccg
    28561 agatacggga cgagagcctt tgagtagaag gaggggaggg tctcggaccg gatcccggag
    28621 ttcgcgagga cccgggcggt ctggggcccg atggcgatga cccgggtcgg cgaggtgagg
    28681 agggggctca ccaccccggc ggggaccgcg ctcgcaaaga agatgcagtc gagatccccc
    28741 cggtttgcgg catccgcgaa ggcgcggaca cggtcgggat agatctcaac cctgaggggg
    28801 gacacggtat agcactcgtg cccgtgcctg cggcaggtct cggcatcccg cgcccccttc
    28861 ccctcgaccc tcgtgaccgc gatgcgcatc gtgaaatctt gcacctctcc tgatatcacc
    28921 gtatccattc agcgggtttt atcccccggt gcccgcaccc tgtatgcgtg gtcgccggta
    28981 tcctcgccgg gtgtatcgtc gggctctccc tgggcaccgt gagcggtctc gtgcccggtg
    29041 tccattccaa tacggttgca tcggccctcc tctccctcca gggacccctc atctccgtcc
    29101 tcgggcccga gggacttgcc gcgacccttt tttccaccct cattacccac accttcctct
    29161 cgagcatccc cgggaccttc ctgggcatcc cggacccgga caccgccctc tcggtcctcc
    29221 ccgcccatgc cctgtgcctg gagggccttg gcgaggaggc agtccggatc gccgccctcg
    29281 ggagcgccct cgccgtggtc gtgggcatcc ccctctccat cctgtgtctc cttctcctcc
    29341 cgccgctcca gccgtacctg gactggggga tgggaatcct cctcacgggg atcgccggta
    29401 ttcttatcgt gacggcggag tcaccctcct gggccctctc ccttttcacc gtgtcggggt
    29461 tcctcgggat cttctccttc cgctactcct tcctcgcctg gcaccccttc ggcgggacca
    29521 ccgtcctcct ccccctcctc tcgggtctct tcggattctc ggccctcctc ctcgcctcgg
    29581 gcgcccccgt cccggcccag cggttcgccg ggatccggat accggggaga accatcgcgg
    29641 cccggaccct tctcggcgcg gtggcgggtt ccatcgtggg ctggcttccc ggcctctcca
    29701 atgcgacggc aaacgccctg gtcgcctcgg ccggcgggta cgaccgggag ccccgccagt
    29761 acatcctctc cgcgagtgcc gcgaacaccg tcaatgcctt cgtgggactc gccgccctct
    29821 acgccctggc ccggacccgg aacggggtga tggtcgccct ggctgcccag gagcttcctc
    29881 ccatgagcac cctgctcatc gccgggtgcc tggccggggg tgtggcatac ctcctcacca
    29941 tccggctgtc gcgatcagcg ggcatcctcc ggggcctcgg gacccggaac ctgaacctcg
    30001 ccgtgatggg tctcgtcacg gtcctcacca tcctcctttc aggccccttc gggctcctca
    30061 tcctccttct cgccacggcc gccggagtgg tgccacccct catcaatgtc ccccgcctct
    30121 acctcatggg ggcggtgatg gtcccggtca tctgtttctc cctcgggatc ctctcggcat
    30181 aaccggattc ggggcggggg ctcgcgggcg tgatcgcggc cttcctcgcc gaaagaaaag
    30241 aagtagcccg gagcagattc gaactgctgt cgcgggatcc agagtcccgc atgattgacc
    30301 gctacactac cgggctattc ccttcagctt aaaaccgccc gattcattaa ggtgacggac
    30361 gggtggagag aggtattacc ggtgaccccg cccggcacac ctgccgcagg ccccgcagac
    30421 ggcttcgacc ggctggatgt cggccccgtc ccggcagaag gcctgcaggt cggtcatgtc
    30481 cctcttataa taaatgtggg gaaccagcga gatgtgtgtg taggggccgc agggcaggcc
    30541 gagggcatct gccacgtact tctccaggtg ggcgagggcg aacatgttcg cacccgccgc
    30601 ggagagcatg tcattggatc ggaagaccac cttcatccgg aggaccccgt ccctcaccag
    30661 gcactggacg agctggaggc acgggcagtc gtccagtttc tcatcgatgg ggggaatcca
    30721 ggtaatggcg atggcccggc gtgagacctt ttgtgtcgcg agcttccgga tcatgtaggc
    30781 cacctgatcg acatgaagct ccctgcccgc cgaggaaagc ccttcccccc agtcgaagag
    30841 gcgcccatgg tagtcgtact cgaactccgc cggggtcccg tggatcaggt catcggcgta
    30901 cttctcgagg aacctctgct ggaagcgcga gcggggggag gccatcgggg cggcgaaggg
    30961 ctcctccacc cggatcccga tctcgtcgca ctccactgtc gcctccccgt cctcggtctc
    31021 gagcacccac cccttctcga gcaccgtctt caccgcgagc tcgtgggccc gaccaaggtt
    31081 cggtgcacgg atgatccgca tggaggagta cgtcattctg aaaagccatt aaggtatgag
    31141 aatcaatccg gcagggaatg tgaggctcgc ggggcacgtc cgttgtcgag aaggaaggtc
    31201 agggcacgga tcgtcgtgat gaaggatgcg gagcgctgtc tttcgacgag aaggtgtgca
    31261 ggggtggggg aacatcccgc ctgtatctca ccgtttaacc cgggattcgg ccgattccat
    31321 tgttttaatg gcataactgg gtgaatgggg ccttcaaaac cggttttatc ccggagtcca
    31381 accacaaaag atatattaac cgattcacaa acactaccac atattccgtt caatcggagt
    31441 atcgggcatt gtgaacacga tttcggcaat cgccgaaatg ctctgacagc atgcctgtga
    31501 atgtaaggag gtaaagcatg aagagtacca tgaagttcat ggtcgcggct ctcgttgtcg
    31561 tggccctcct ggcggtaccc gccatggcga ggacacccga cgttaacacc cttgtagcgg
    31621 gagacgtggc ctttgtctat gaaacgaacc tcaacgtgcc agccgtcggt ggaggaaccg
    31681 tggccgttgc gaagttcggg aatgactgcc ccgtgtcagg tgcaggatgc actttcacag
    31741 gggagctggc gcggattacc ttgaacccgg ccggaaaact ggacattaac acggcgcccc
    31801 cgtacttcgg aacctggttc ccgattgacg caactggcaa tggcgcctta gggaattcct
    31861 tccagatcca gcacgcggat atccagcttg atgccgtcct gaatgcgtcg cacatcgaca
    31921 ccatcagcgg gaagagtgtg acccgggaca cgaatatggc gttcaagttg atcacgaact
    31981 acgctggcaa ctactacaag gtcggtagca cctacctcgc caaggtgaac atcgagctca
    32041 ccacgcccgg cggtggtaag atcatgatct tcgggaacaa tgccgttgat cttcgtgcaa
    32101 tcaccttgaa cgggagtgtg gagtacactg acactcttcc ggcaggcagc caggcagccg
    32161 gggtaggtaa tgccattgac ctgaccaata tggaggccgg cacctacacg gcgaccgcga
    32221 agtggacctc gacaatgccc tggttccagc tggagagcaa ctccaatgca gttaccttca
    32281 ccgtgctgtc caagcccatc accgtcacga ccaacaagga gtcggtggtc aggggcaaca
    32341 ccttcgtggt gaccatcacc ggtgagagca agcacctcta ttacgtgtac atgaaggacg
    32401 cggccgtcac ctcgggatac atctacccgc agatcacgaa cggccagtcc aacgtgttcc
    32461 tcacggcgct caaaatcaat aactgcgcaa tcgcaggatg cactgccgtg gccaatgacg
    32521 tcaacacgtt gtacggcacg gtagtcgcca acaacgcgac tggagcagtc gtcaacacct
    32581 cggccgatgg cacacgggcc atccagttcc agacgagctc ccaggcgagc ctcctgacct
    32641 atgacaccaa gtacaccatc aaggtcatgg actcgcagga cgcaaccaag tatgacacgg
    32701 tcgatgtgaa cgtggccaag gggcaggtca ccattaccta tgagggtacc gggacctact
    32761 acctcggtga gaccatcaag ctgaccggga cgaacaccga cagcaacgcg gtattcctgt
    32821 tcctcgttgg cccgaatctc gccaccaacg gtgtaaacct tgcggatatt tcccagaaga
    32881 cccaggatac cgttgaaggc agcttcaaga aggttgacgt caacaccgac aacacctggt
    32941 ccttcaagtg ggacacctcc gcgctcacaa agacgctgga tgcaggaacc tacaccatct
    33001 acgccgccgc ccaggcccat acgaaggata acctcacgaa cacgcagtac agcaccgcct
    33061 cggtggtact gaagaagccc ttcgtgacag cctcggcaag ctcgaacacc cttgcacagg
    33121 gtgacatcct ctacatccag gggaccgcag aaggcaagcc gaacaacgtc tacatctgga
    33181 tcttcggcaa gaactaccgg gactgccagg tgtctgcgac cgtccagtct gacgcgagct
    33241 actcctacaa gctggaccgc ggggcgaccc acgacctctt cgccggccag tacttcgccg
    33301 tcgtccagca cccgatgatg aacggtatcg ccgatatcct cggcgacccg acggggacgg
    33361 gctgtcttaa cgcgactggc acgaccaacc ccgtcaaggg aacctgggta ggcggtgcgc
    33421 cgaccgtgag gctcgttggc ctgcaggcct ccgatgccgc gaccgcgctc gttgacctgc
    33481 tcaactcccc gaacgttgac gacacctaca ccaagctgtc gttcatggtc gaggcgccct
    33541 ggatccggat cgacaccatc agcgaccact acgttggtga caccttcacc atcaccggta
    33601 ccaccaacct cgcagagggt gaccagatcc tcatgaccgt cgtctcctcg tcgttccagc
    33661 caaccgagaa gactcagacg ggcgagttca gcggcacctc cggcacggtc accgtgcaga
    33721 agggcgacac ctacaacacg ttctcgttcg atgtcgatgc ctccaccttc aagcctgacg
    33781 agtacctcgt caccgcggag tccatcgagc cgagccagac cgtgaccgcg accttcaatg
    33841 tccttgaggg ccagcccacc acggcagtga ccaccgcccc gccgacacag gcgacgactg
    33901 cacccgttac caccgcgccc acacaggtag ccaccaccgt tgccacgact gccccagcgc
    33961 agcccggctt cggcgccctg gttgccctcg ttggcctcgg agcggttgca ctcctcgtcc
    34021 tgcgcaggcg ctaaacgaaa accacctttt ttttgcagtt ccttcaatcc ctgttcttat
    34081 ccgatctccc ctgtatgggt tttccttcct gacctgatcc cccgagcaaa aggtgtcccc
    34141 ccgctcgcag aggaaggata gatgtccttt ccatactgcc cccgatgggc agggagcccg
    34201 ccgccaagct tctttacgat gagaaccaaa ggttactcca gagagggtga cttaccaccg
    34261 atgaaactca cggtaaagct catggatatc gcggccccgg gggtgctcct gcaggagaac
    34321 gatgcccgga ccatcggcgt gctcgaggga gaccgggtca gggtcctcaa cgaggcgaac
    34381 agcgaatcgg tctcggccct cgtccatacc acctcggggt tccttccgca gggcaccatc
    34441 ggggtgtacc agaaggccaa cgggcgcctg ggcttttcag acggggcgag cgccgaggta
    34501 agggagacgg ggaggctctc atcgatcgat tccatcaagg gcaagatgga tggcaaacgc
    34561 ctctccaagg aggagatgtt cgccatcgtg aaggacaccg tcgaggagaa tctctcgccc
    34621 gtcgagatga ccgcctttgt cacggcatcg tacatccacg ggctcgacat gaacgaggtg
    34681 gagcacctca cccggtccat ggtggagacc ggcgaccgca tccagttctc gacccatccc
    34741 atcgtggaca agcactccat cggcggggtt ccggggaaca agatctccct gctcgtggtc
    34801 cccatcatcg ccgcaagcgg gctcaagatc ccgaagacca gctcccgggc cattaccggg
    34861 gccgccggga ctgccgacct catggaggtg cttgccccgg tcgagttcag ctccaccgag
    34921 gtaaagagga tgaccgagaa ggtggggggc accatcgtct ggggaggtgc gacgaacatc
    34981 gccccggccg atgaccggtt catcatggtg gagtacccct tccggatcga cgcgatcggc
    35041 cagatgcttg cctcggtgat ggccaagaag tcggcggtcg gggccgacct cgcggtcatc
    35101 gacatcccgg tggggaacgg gaccaaggtc ccgacggtcc aggacgggag gaggcttgcc
    35161 tcccagttca tggagctcgg cgagaggctg aacatccagg tggagtgcgc cctcacctac
    35221 ggcgaatccc tcgtgggcca tgccatcggc ccgaacctgg aggtgaacga ggccctttcg
    35281 gttctcgagg gggcgcaggt ccccgggtcc ctcatccaga agagcctcgg cctcgcggga
    35341 attgctctcg agatggccgg gaaggcagcc cggggccagg gatatgccgt ggcagaggga
    35401 atcctttcga gcgggaaggc cctcgagaag ctccggcaga tcatcgagat ccagggtggg
    35461 aacccgaagg tgcaggcgag cgacctcgtc cccggggagc atcgcttcgt ggtgaatgct
    35521 cccctggacg ggtacgtcgt ggagctgaac aaccgttccc tcatcaccat ggcacgggcg
    35581 gcgggggcac cccatgaccg gggtgccggc ctcatcatcc atgccaagaa ggggacgaag
    35641 gtgaagcagg gggagcctat catcaccatc catgcggacc ggagctggag gcttgacaag
    35701 gccgtcgagg tcgggaggca gctcatgccg gtgctcgtcg aggggatgct cctcgggcat
    35761 atctccccgg tccactggat ctagatctcg ttttttctcc caacaactca aattcccgag
    35821 cccaccagat aaaagcgtcc tccggcggca gtttctatga atatttcatt atatttaaat
    35881 atttaatctg tccaaaaagg agatcctgtg ataaaaatgg actttaccaa tatataatgc
    35941 tcaatttaac aaaatcacga tttaaagcaa attctcttta taggatagtt ttaagtaaaa
    36001 gagtgattaa tactatgcat ctgtgaggtg ggagcaatga ggctctatat cttagcactc
    36061 ctgatcatct tcctgtccac catctcggcc gtggccgacg cccaggtcgc cccggatcat
    36121 atgattatct ccggcaagga ctggatggtg gcaaatggca tggatacctc gaccatcacg
    36181 gtgaccgtgt acgatacggc aggaaatccc ctccccctcg tctcggtcac cctccaggga
    36241 acccctccct ggaccctctc ggccacgagc ggggtgacag atcccttcgg ccagatcacc
    36301 accgtcctcc agcctaccac ccggagcggc tcggacccca tcaccgtgaa tgcctcggtc
    36361 atcgaggccg gcgtattgaa atccatcgag caggtatacc tgcagaacat cgaccacagc
    36421 attccccaga cggctgtccc cacctacgac gtcaacgtgt ccatcggggg aaccacctcc
    36481 ctccaggtaa aggtgacaga tgcctacgga aacccggtag acaaccggaa tatggttgag
    36541 acggtccagt tcaccacctc agggtcgacc ggctcgggtt tctgggatgg aacctcctat
    36601 gtgaagatac tgacggcccc cgttgactcc ggcggtattg cccatgcgac cttccttgtg
    36661 gcccagtccg gtgccaactt cgtaaacatc cagcctccct cccccatacc cagccggttg
    36721 atcaccatta ccgggatcaa tgatggctca ccctatgcca ttacccaggc cgtctccccg
    36781 ggcggatatc cataccccta tacgagtaca gacgggagca agttcagggt tctctacacc
    36841 cttctcgacc agttcgggaa cccttcggga aacagggggg tcgtgataac cacgagtgtc
    36901 ccgggcgagc agatgactct caccacgaac agctatggaa ttgttgcatt ttactatcag
    36961 aaggatatcg ccgggctcta caccctgacg gcaaccgccg tcgacaactc gagcgtgacg
    37021 gcctcgcaga cggtggaatt cgtggccgcg aatcccacca ccatgctcct caccgcgagt
    37081 ccccagacga tgccctcccg ggacgtggtg gcctccacca cctccaccct ctttgccaag
    37141 gtcatcgacg atatggggaa cccggtgaag ggggaggtgg tgagcttcac cgtctggtcg
    37201 tacaaggtcg ggatctacga ccagaccgtg aacccatcca tcgagaacga gactaccgtg
    37261 accgatatcc cccgcaccgt gctcacggcc acgacagata ccgacgggct tgcccatgtc
    37321 accttccggc ccggtgcatt cacgacgaac cggtcggatt ccaagtacag ctacacggcc
    37381 cagggaactg ccctcatcca ggccacctgg ggatctgtta cccagaccat tgaactccag
    37441 tacatgaact accccttcct ctctgtcggt acgtcggtgg aaccgaagac ggtcgtcgtc
    37501 aacaatacgg taaacatctc cgtccagctc aggggagatg gctgggccct ccagccgaag
    37561 cccctcgacg tggtgctcct gtgggaccgg tcgggatcca tgctctttaa cgagactctg
    37621 gacacctcaa agagtccaga cctgattacc tcggaatccc ccgacgaccg cctggtcatg
    37681 gccatggccg ccggaaagct ctttgtcgac aagatgaaca gttcggaccg ggtcgggctc
    37741 gtctccttcg gggatctgag cgggaccagt ggatgggctg tcctctacca taccgggaac
    37801 agcacctccg attcccacct gtacagtctc ggctggcgtg cgggcagaga ctataccctg
    37861 gataaatatg ggaacggcca gcagtactcc accgatgacg cgacctacgt gacctcgcgt
    37921 tacccgggtc acggtgcctg gggaaagtac tacgggtctg cgatggcaac gaccgacctg
    37981 aacctcagct acgacaagtc gacggtaaag aacatcgtct ccaacatggt tccgaacgga
    38041 ggcaccccct tgcggcaggg cctctatcag gcggtcaggc agatcatcag tgacccggat
    38101 atccttgccc acaggcggga caatgccatc agggccatcg tgctcctcac cgatggcgcc
    38161 tggaacaccg gcggtgaccc gaggggtgga tccggtgcgg tctcgcttcc ggggataaat
    38221 accgggagcg tcatcaccct cgcggcgcag aacaacatct ccatctacac catcgccctc
    38281 ggatcagagc cctacaagga tcagctggcg tcctatgcca accagaccgg cgggaaggcg
    38341 tacattgcga attcaggcct tgaccttgac tccatctacg cctccatcgc cggcgagctc
    38401 cacacccagg ccggggtcgg aaccgccatg gacctctcct tccacaccat cgacgtgaat
    38461 ggggccatga tccccggtat cgatgtcctg aagtacgtac ccaagaatcc gtattcgacc
    38521 catgaattca gttacaatgc aacgatgccg gtcatcgtca accggtggta cgaccagacg
    38581 acggactggg atacggacca gaacctctcc ttcaacgtcg ggaccatctt catcgggggg
    38641 gtatgggagg cgaacttctc catgaaggtt ctcaaacagg ggaacatcaa gatccttgac
    38701 tcgaattcct ccatcagatt caacgacgga tccagcctcc ccattccgga cacctacatc
    38761 acctcgctcc cgaacagcac agggatcggt ctcgagggta ttggtatccg ggtaaccgat
    38821 gttcgcagga cggacacggg cacgaacccg gactacgtgg atgtcgcgtg gaacctcgcc
    38881 tacaccggtc ttgagccctt catccaggaa tatgtcgaca tccggcagga aggaaccctt
    38941 gaatggaacc gccgcatggc gaaaacggtt gacccgttca cgacgagcga tgccttgacc
    39001 ctgtctattt caagccttcc cgcgggcatc tgggaggtca gggtcacggc ccatgccgac
    39061 gatgcgggcg atgatgcaat gtccacacag ttcgccatcg tcaaggcaga ggggacctcg
    39121 aagatcaaga tcacctgacc ttttcttttt tctaaccagg tggatttttt cctttcttat
    39181 ctggttctcc tgagggttcg cctaatccta cgcccacgtc taaaactaac accagatcgg
    39241 gaactgaaca gctgacaatc cgacgctcct gggggactcg ctccactcgc ccccgccgct
    39301 tcggcgtccc ccgaatggcg atgggcttct cattccgttg aaccgtcgga tcacatatcc
    39361 cgatacacgg agggggatgg ccgcgccggg gggcgatagc ctcgcgactt cgtcgctaga
    39421 ggtcggtttc tacgcgactt cgtcgctcga cgtcggtttc ttacgaaacc tccctcttac
    39481 gaaacctccc tcccccccaa gagcctgaca ataataaccg gatggctgtt attcaccgat
    39541 ttgcatcgag aaaaagaaaa tgactagggg tcgcatgact ccggaggaac tcgtattgac
    39601 aggaagctaa aagttcagga aactaaactg gggcggagcg gacccagcgg gaatcgaacc
    39661 cgcgtccttg ggtctcttct tccggtccgg aatccgaagc ccaagaggat atcctctacc
    39721 ccatgggtcc agttcccggt ttctttcctg cctgtattaa ctattccccc ggccggctcc
    39781 tcccccaccc ccgacggctt tatccaaacg ttaaaacgtg gccgccccca agtacgtggt
    39841 aggatgatcc tctcagcttc cgagatccgg cgaaggcttg gggagccggg cgatctcgtc
    39901 atccgcccct acagcgagtc atgccagcag ccggcctcct atgacctcag ggtgaccggc
    39961 caccagatcc tgccgcgggg gtcgtgtacc ctcattccca gcctcgagtg ggtggagata
    40021 ccgggagacc tcgccgcgac gctcaggtgc cgttcctcgt atgcccgccg ggggctcgtc
    40081 ctcggcggag gcttcgttga cccgggattc cgggggcagc tcaccctctg cctcgggaac
    40141 ctgagcgccg gggacattgt cctcgcaccc tcggaaagag tggtccagat gatcctccac
    40201 caggtcaccg ggggagggga gctctacggg ggccggtacc aggacagcca gggggtagtt
    40261 caggcgcgat gaggctcatc ccgagtgaga ggaagtatct cgagatcctc cggatcctga
    40321 aggagcacca ggatccgatg ggggcgaaga ggctctcgga gatccttgcg gagcggggct
    40381 tcgtcctcac cgaccgggcg gtgcagtact accttcgcta cctcgacgag atggggttca
    40441 cccggaaggt cgggaacacc gggcgggtcc tgacccggga ggggatggcc gagaccgaga
    40501 gcgccctcgt cggtgagcgg atcgggttca tcatctccaa gctggagcgg ctcgccttcc
    40561 ggagcacctt tgaccccgag acaggcaccg gcgatgtggc ctacaacctc tccctcgttc
    40621 ccgaggggga ggtccccggg gtcgcccgct cctttgagaa ggtgtcgaag gccgggtacg
    40681 gcttttccac gtcctaccgg atcctggacc gggatccccg ggtcccggag ggacatgtgg
    40741 ggatcatctc gctctgcagc atcaccatcg acggggtgct gcagcgttcc gggatacccg
    40801 tgaccatggc ctacggcggc cggctcgggt ttgagggcgg ggtcgtgaaa gggttcctcg
    40861 acctcatcgg ctaccggggg acgaccatcg atcccctcca gctgttcatc tcggctggcc
    40921 tcacctccat ccgggaggtc gtggcctcgg gaaacggggt ggccctggcc aacgtgaggg
    40981 agattcccgt tcctgccctc gatcatgtca cagacctcct cagagagatg ggccggtggg
    41041 ggttcacccc gccggtgagc atcgggaccc gggccttcaa cgccccgtac accccacacc
    41101 acgtttccgt cgtatcgtac agcgggatga acctggtcgg tcacggggtc gaagagggct
    41161 accacatccg aaccgagatc ggggccggga acatccagtt ctcgaagatg gagccggggg
    41221 ttgagaaggg taaccggtga gaatccggtt cccttccggg gaactcgtga acccgcagag
    41281 ggacgggaaa atggggtgtc ttcggggaga acacccggtt ctcaattatt tcgctcctct
    41341 tcccgcggga ggcaaggatt tccattgcat ggccgtttcc cctggattcc tgtggcgaat
    41401 catgattttc ccccatatca gggttcgcaa ccctcatata ggggaaaact ggattatctc
    41461 agggcctccc ctttcttcgg gtatggaatt caggggagaa atcatgggtt tcatggtaaa
    41521 agactggcga taaagcctcc tttgggatgc cacgagaaaa atgcttaaca ctagaaatcg
    41581 tatataaagg taatttctaa caagataacc catattaatt gtcaaattcc actaaacaga
    41641 cctataaagt gctttaatca agttatctat ccataatccg ctcattcaca cagcgtaatt
    41701 gttatatata tggacgagaa taccagttat gcacacggca gagaggtgaa taccacgagg
    41761 tacatttcga tacttaccat cctgttcctc ctctcgcttc tggtaggtat ccctgtggcc
    41821 gccgcggtga ccgtcgaggt ggtcagctcg caaaagtgga tcaccgcaaa tggtttggat
    41881 tcagcctata tcagtgtcag cgtcaccgat gggggcaagg ccgtcagcgg ggcggcggtt
    41941 gtcctcagcg tttcgagccc ctgggtcctc agggacaccg gaggaacgac cccggccggc
    42001 gggcagatag cgacggtttt ccttcctacc acgaagtccg gcaccgccga aatcacggcg
    42061 accgtgaccg tcccgggttc cccgatcccg gttatcggga actattccca gaagatcgat
    42121 gcctctcatc ccatgagggc agagaagaag tataagatca ccgcgtcggt gggatccatt
    42181 accgacatca ccgtacgggt aacggatatc tacgggaacc cggtcacgag cctcatggat
    42241 ccaaatctgg tcaccttcaa caccacctcg agcggggaca atacctttgt ggatccatcc
    42301 tccaagaagg tcaaggtaaa gaacctcgcc atcgccctga atgacaccgg ctatgccagt
    42361 gtcggtttcg ccgtcagtac ccatcccggt gacaacttcc tctatatcac ccctcccagt
    42421 ccgcttccgc cgtccctgat caagatccgg ggaatcgctg acctgccccc cgcctccatc
    42481 acccagaccg tcacccccgg ggggaagccc cccaccctcc ccacgtccac ggcgaaattc
    42541 atcatcgagt acgagctcac ggacacatat gggaacccct ccacgaacca gtatctcaac
    42601 atcagcacga atgccggtga gagccggacg atcctttcca acaatgcggg gaatgtgatt
    42661 atctcgtacg gcccgaaggc cacggcgggg aggtacacca tcaccgcgac atcgcaggcg
    42721 gaccccaacg tatccgtggt ccagctcgtc cagttcctgg caggcgatcc cacgaatatg
    42781 ctcctcaccg caagtcccca gagcatggca agccttgacg tggacccggg caccgtgagc
    42841 agtctcatcg cgaaggtcgt tgatgcctcg gggaacccgc gcgggggtca gaccgtctcc
    42901 ttcagcatcc agcctccggt gaagaacgga tcctatatcc agaccagcct cccctacctt
    42961 gacaggaaga cctatactat cggggatgag gtcactgcga ttaccgatga gaacggcctt
    43021 gccaccgtgg acttctaccc cggtgccttc ttcagtaata cgagcgatcc ccggttcagc
    43081 accatggccg tcgcgaccgc cacggtccgg gccaagtggt catcggtgac gaggacgatg
    43141 gatctgagtt acaagaactt cccctacctg agcgtgtaca cctcggtgga cccgagcacg
    43201 gtggagacaa accgtacggt ggatgtcacc atccgggtga agggcgacgg ctacgctctc
    43261 cagccgaaac cggtcgatgt ctacatggtc ctcgaccgtt ccggatcgat gctcacggat
    43321 taccaggacc ggatggtggt cctcatgaag gcagccgaga ccttcgccac caagttcaac
    43381 tacaccaccg accagctggg gcagttctcc ttcggggaaa agggctcggt tgatgccacc
    43441 tgggacaaca actgcgggaa ggacggagat tccagcgatg acgccgcata cgccagcaag
    43501 aactatcccg gaagtcccag gtcctacagt gattatgcat tcatggacat cggactctcc
    43561 aggtcccctg caaatatcgc ctctgccatc aaggccacgg tccccggtgg ctacaccccc
    43621 atgcggtatg ccctcaagca gtccatcgcc gccctcatca acgacaagaa caagggcgtg
    43681 gtgaaggccc tcgtgctgtt ctcagacggg gactacaact atttcggcga cccccttgcc
    43741 cgcggctcgg tgggttccac ggatcccacg tcatacgatg acctgaaccc ggactatgtc
    43801 tccttcagcg gtgtctcctc ccagaacatg gcggtttacg cgaaggcgaa caatatcaag
    43861 atcttcacca tcgggtacgg ggcccagctc tcctcaggcg gcagggacac actccagaag
    43921 atcgcaacca ccacgggcgg gaagtactac tatgcatcga cgggagacga tctcgccagc
    43981 gtattcacgg atatcgccgg agccctgaag gacacggccg gggtggccac gtacgttgcg
    44041 ctggacttct cgaaggtgga ggtgaccgat gtgaaggggg tccaggtcgg cacgggctcg
    44101 agcgtcttca agtacgagcg gatcccgtgg aagtcgaccc
//
LOCUS       DATGDI010000009         3608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_262683, whole genome shotgun sequence.
ACCESSION   DATGDI010000009 DATGDI010000000
VERSION     DATGDI010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262683"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VKO45_00510"
     CDS             complement(<1..621)
                     /locus_tag="VKO45_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HMA04390.1"
                     /translation="MAHIHLPDGSFTLSWVVFWWLCALILLGVCLALARRRAVDGRRI
                     TIAAFVTAAAFAAFVIEVPILGGVHLNLTPFIGILVGPVLGGLVVFVVNVLSAAIGHG
                     GWGLIGANVLVNFSEVATGWLAYRGLIRLTPGRFARAGVATFTGLFVGNLAEVAIILV
                     SGVQGVAQTGSQLIPGLSLIVAVNLGAAAIEALVTGFLVEYIGRARP"
     gene            complement(624..1520)
                     /locus_tag="VKO45_00515"
     CDS             complement(624..1520)
                     /locus_tag="VKO45_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA04391.1"
                     /translation="MTITRRDLSTGGACPILDPEREIIHVDCASHQYPDGSVGIHDMC
                     FHVMKDEIVAICGPNGSGKSTLIEHLNGLLTPSEGKIMVRGKALGEGEDSSLWRVVGV
                     VFQEADDQLFAPTVLDDVMFGPLNLGLTREEARERAKGALEAVGGSHLAGKLPNYLSG
                     GEKRLIAIAGIVAILPRVIALDEPTSDLDPVNSEVIERLIVRLKEEFGIGVVLATHDL
                     DLAARIADRICLVRHGSVFAEGPPDEVFYDEELVREAGLRMPSGIQLYLDLCRSKGIA
                     PERRPVRREEIVAAMKEIGKRA"
     gene            complement(1525..2382)
                     /locus_tag="VKO45_00520"
     CDS             complement(1525..2382)
                     /locus_tag="VKO45_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HMA04392.1"
                     /translation="MPHDPERFREEEPAVSSLPLSSHLPDLDLITYYAERRESFFTRV
                     SPWTKAFLLATIIVTVTVVRNPVLLLVLFLAVLALFVLAGLPVGKLLSWYMYPLVFVL
                     SLVGILAWTQPGTPVIGLSAVGIPVILTDQGLLLVATLTGKTLISVTFTFFFLMTTRY
                     KHLSGMISRVLPAPLDQIFLMAYRFFFLTLAMLGAILKAVRSRGGTLVKGFLTQGALY
                     AEIFGLVFLRSLDRAERVNKAMIARGFTGTYPSMADLPAPGLAEAGGLAVCVALLAAA
                     LLAVPGGGW"
     gene            complement(2337..2789)
                     /locus_tag="VKO45_00525"
     CDS             complement(2337..2789)
                     /locus_tag="VKO45_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HMA04393.1"
                     /translation="MQAEDGLGLSPRKVEYLKYILEMGGQAKTTRIATRFRVDPSTAS
                     KTIEELARSGLLVHEPYRGVTFSEKGRRCAEFLVRRHRILALALSRLGLPGEEACAEV
                     SRFESRVSRSAVDHICRAMGHPREGICGEITHGSCRMTRRDSARRNRP"
     gene            2946..3020
                     /locus_tag="VKO45_00530"
     tRNA            2946..3020
                     /locus_tag="VKO45_00530"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2982..2984,aa:Glu,seq:ctc)
     gene            complement(3055..>3608)
                     /locus_tag="VKO45_00535"
     CDS             complement(3055..>3608)
                     /locus_tag="VKO45_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HMA04394.1"
                     /translation="SMPNFHARIPSSKLQRIPSPGVTIPKANPPAPRSFTGEGDREKI
                     TPEIQAKVWKKRFGFRKTGTCVCCHQRTIYRDDFVCGHRRTYAGGGSNDVSNLEPICG
                     ECHEKMGTQDLVSWCKKYREELNSVKPMPKTTTAKKPVRKMTAKKPVRKTAAKRPASR
                     TAAAKKTVRKTTSRKPAAKKSKR"
BASE COUNT          686 a         1119 c         1158 g          645 t
ORIGIN      
        1 cggcctggcc ctgccgatgt actcaacgag gaagccggtg accagtgcct cgatggccgc
       61 cgcccccagg ttcaccgcga cgatgaggga gaggcccggt atgagctgag agcccgtctg
      121 ggccaccccc tggactcccg aaacgaggat aatggccact tcggcgaggt ttcccacgaa
      181 tagaccggtg aaggtggcga ctcccgcacg ggcgaacctg cccggggtga gacgtatcaa
      241 tccgcggtag gccagccacc cggtcgcaac ctcggagaag ttcacgagca cgttcgcacc
      301 gatgagtccc catcccccat ggccgatggc cgcagagagg acgttcacga cgaagaccac
      361 gaggcccccg aggaccgggc ccacgaggat cccgatgaag ggggtgaggt tcaggtgcac
      421 cccgccgagg atgggaacct cgatgacgaa ggccgcgaag gccgccgccg tcacgaaggc
      481 ggcgatggtg atcctcctcc catccaccgc ccggcgccgg gcaagggcca ggcagacccc
      541 gagaaggatg agggcgcaga gccaccagaa caccacccag gagagggtga aggaaccgtc
      601 gggaagatgg atgtgggcca tggtcacgcc ctcttcccga tctccttcat cgccgcgacg
      661 atctcctctc tcctgaccgg tcgcctttcg ggcgcgatgc ccttcgaacg gcacaggtcc
      721 aggtagagct ggatccccga tggcatcctg agccccgcct cccgcacgag ctcctcgtcg
      781 taaaagactt catcgggggg gccctcggca aagaccgacc cgtgccggac caggcagatg
      841 cggtccgcga tccgggccgc gaggtccagg tcatgggtgg cgagcaccac cccgatcccg
      901 aactcctcct tcagcctcac gatgagcctc tcgatcacct cgctgttgac cgggtcgagg
      961 tcggaggtgg gctcgtcaag ggcgatgacc cggggaagga tcgcgacgat cccggcgatg
     1021 gcgatgagcc gcttctcccc gcccgagagg tagttcggca gcttgcccgc caggtgggat
     1081 cctcccaccg cctccagcgc ccccttcgcc ctctcccggg cctcctcccg ggtgagcccc
     1141 aggttcaggg gcccgaacat cacgtcgtcg agcacggtgg gggcgaagag ctggtcgtcg
     1201 gcctcctgga agaccacccc gaccaccctc cagagggagg agtcctcccc ctccccgagg
     1261 gcctttcccc tgaccatgat ctttccctcg gaaggggtca ggaggccgtt caggtgctcg
     1321 atgagggtcg atttcccgga cccgttcggc ccgcagatcg ccacgatctc atccttcatc
     1381 acatggaagc acatgtcatg gatcccgacg ctcccgtccg ggtactggtg gctcgcgcag
     1441 tccacgtgga tgatctcccg ttcgggatcg aggatcgggc aggcaccgcc ggtcgatagg
     1501 tctctcctcg tgatggtcat ggggtcacca tcctcccccg ggtaccgcga ggagcgcggc
     1561 tgcaagaaga gcgacgcaga ccgcaaggcc tcccgcctcg gcgaggcccg gggccggcag
     1621 gtccgccatg ctcgggtacg ttcccgtgaa cccccgggcg atcatcgcct tgttgaccct
     1681 ctcggccctg tcgagggaac ggaggaagac gaggccgaag atctcggcat agagggcacc
     1741 ctgggtcaaa aagcctttga caagggtccc tccccgggac cggaccgcct tcaggatggc
     1801 cccgagcatg gcaagggtga ggaagaagaa ccggtacgcc atgaggaaga tctggtcaag
     1861 gggggcgggg aggacgcggg agatcatccc cgagagatgc ttgtaccggg tggtcatcag
     1921 gaagaagaag gtgaaggtca cggagatcag ggtctttccc gtcagggtcg cgacaaggag
     1981 caggccctgg tcggtgagga tgacggggat ccccacggcc gagagaccga tcacgggcgt
     2041 ccccggctgg gtccaggcaa ggatccccac gagggagagg acgaagacca gcgggtacat
     2101 gtaccaggaa aggagcttcc ccacgggaag gccggcgagg acgaaaaggg cgaggaccgc
     2161 gaggaacagc acgaggagga ggaccgggtt cctcacgacg gtgaccgtga caatgatcgt
     2221 cgcgagcagg aatgcctttg tccaggggct cacccgggtg aagaaggatt cccggcgctc
     2281 cgcgtagtag gtgatgagat cgagatccgg gaggtgggac gaaaggggaa gggagctcac
     2341 ggccggttcc tcctcgcgga atctctccgg gtcatgcggc atgaaccatg ggtgatctcc
     2401 ccgcagatcc cctcgcgggg gtggcccatg gcccggcaga tgtgatcgac ggcggaccgg
     2461 gacacccggg actcgaacct cgagacctcg gcgcatgcct cctcgccggg gagaccgagc
     2521 cgggagaggg caagggcgag gatccggtgc ctcctcacca ggaactcggc gcacctcctt
     2581 cccttctctg agaaggtcac cccgcgatag ggctcgtgga caaggaggcc gctccgggcg
     2641 agctcctcga tggtcttact tgccgtcgat ggatcgaccc ggaaccgggt cgcgatccgg
     2701 gtcgtctttg cctggccccc catctcgagg atgtacttga ggtactccac cttcctcggg
     2761 gagaggccga ggccgtcctc cgcctgcatg ggttgtacct gtcatcgccg ggagaaaaaa
     2821 gttggggtat ggccaaattt tgaaaccccg gtagaagaca gggatgggca gaaaagacgg
     2881 gaaccgtgac ggagaccggc gggcacggat atgtttaagt aaaaatgtac cgaaggttat
     2941 agggcgctgc tatagtgtag accggccaat catgcgggcc tctcacgtcc gcgactgggg
     3001 ttcaaatccc catagcagca tcttttctgc gacgctctga ttccagagga tgttttatct
     3061 cttcgacttc tttgccgcgg gtttccttga ggtcgtcttc cggacggttt tcttcgccgc
     3121 ggctgttctc gacgcgggtc tctttgcggc cgtcttgcgg acgggtttct ttgcggtcat
     3181 cttccgaacg ggtttctttg cagtagtcgt ctttggcatg ggtttcaccg aatttaattc
     3241 ttcccggtat tttttacacc atgataccag gtcctgcgtc cccatcttct cgtgacactc
     3301 gccacagatg ggctccagat tgctgacatc attactcccg ccgcctgcat aggtccggcg
     3361 atgtccacac acaaagtcat ccctgtatat agttctctga tgacagcaga cgcatgtgcc
     3421 tgttttacga aagccaaacc tcttcttcca tacctttgcc tggatttcgg gagtgatctt
     3481 ctcccggtcc ccctctcccg taaaactccg gggagcgggt gggtttgcct tgggtatggt
     3541 aacaccgggt gaaggaattc tttgcaattt ggatgatgga attcttgcat ggaagtttgg
     3601 catagaag
//
LOCUS       DATGDI010000010         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_88260, whole genome shotgun sequence.
ACCESSION   DATGDI010000010 DATGDI010000000
VERSION     DATGDI010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88260"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1515)
                     /locus_tag="VKO45_00540"
     CDS             complement(112..1515)
                     /locus_tag="VKO45_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HMA04395.1"
                     /translation="MRLPPKMLRRHEVMTGDTVSIIGLGKLGASMAAAMASRGLKVIG
                     VDVVPRAVELLNQGKPPVVETDLAEYITRHRKNLEATADYRHAVQNSDITFVIVPTPS
                     DERGAFSIKYAISAFQSIGEALREKDGYHTIVLTSTVLPGSTRFGLLPVLEEASGKKC
                     GKDFGLCYSPEFIALGSVIHDFLNPDFLLIGEFDKKSGDMLTGYYSRIMENNPPVKRM
                     SIENAELTKISLNSYVTMKITFANMVSALCERIPGGDVDVVTDALGLDRRIGRRYLTG
                     GLGFGGHCFPRDNIALAFLARSLGVSHELLLATDHFNKNIPGEAVKKLGGLIKPGMTV
                     GILGLSYKPLSCVVEESQGILLAESLAAHDVKVLGYDPLAREEVVHKYLGKIEMVYSL
                     QECIDSSDVVFITTPDPAFKDLRKEDFSRGRNPKVVVDFWRMLKGELEGGQVKYIQVG
                     TGEMEGEALENLENLWR"
     gene            complement(1589..2143)
                     /locus_tag="VKO45_00545"
     CDS             complement(1589..2143)
                     /locus_tag="VKO45_00545"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HMA04396.1"
                     /translation="MKCWEVLDRECVADAAPWMRLVREKVRLEDGRVIDDYYTIEEPD
                     FAVVFAVTPRDEVVGIWHYKHGPQRINLGLPAGYVKEGEAPLEAARRELMEETGFSAS
                     GWRHLGSFSVHGNRGCGKAHVFLARNAVREGDPTPDDLEEIRVELIPLTALREHLRKG
                     DVATLGAAAGMALGIASYMRDEEK"
     gene            complement(2159..>2667)
                     /locus_tag="VKO45_00550"
     CDS             complement(2159..>2667)
                     /locus_tag="VKO45_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMA04397.1"
                     /translation="YGPKGSWVGGREKAPAAFCRKVAVAKLSGDPSIDIWGDGEQTRS
                     FCYIDDCVLGLMKLMASDFHDPLNLGSDRMVNMNELVGIIAGIADTQVEIGHIEGPQG
                     VRGRNSDNTLLRKVLRWEPEISLEKGLGITYRWIEALVRDTLDHAPDRKKAIEELRES
                     RIYQAPCT"
BASE COUNT          488 a          860 c          777 g          542 t
ORIGIN      
        1 catttggcta ttcgctggtg aacctttcaa atgttcgact cgataacgga tgccaaataa
       61 aatgggcaga cggtttattc gtatatcgtg gatggaacaa ataaactagc cctatctcca
      121 gagattctcc aggttttcta gggcttcacc ttccatttcg cccgttccta cctgtatata
      181 tttcacctgt ccaccttcca gttccccctt cagcatcctc cagaagtcca cgaccacctt
      241 cgggttcctc ccccgggaaa agtcctcctt ccggagatcc ttaaacgccg gatccggcgt
      301 agtaataaac accacgtccg acgagtcgat gcactcctgg agcgaataga ccatctcgat
      361 cttccccaga tatttatgaa ctacttcctc gcgggcgagg ggatcatagc caaggacctt
      421 cacgtcatgc gcggcaaggg actcggcaag gaggatcccc tgggactcct cgaccacgca
      481 ggagaggggc ttataggaga gcccgaggat gccgacggtc atgcccggct tgatcagccc
      541 cccgagtttc ttcacggcct cgccggggat gttcttgttg aagtgatctg tcgcgaggag
      601 gagctcgtgg gaaaccccga gtgatctcgc gagaaatgcg agagcgatgt tgtcccgggg
      661 gaagcaatgg ccgccgaacc cgagcccacc ggtgagatac ctccgcccga tccgccggtc
      721 gagaccgagg gcatcggtga ccacgtcgac atccccgccg gggatccgct cgcagagcgc
      781 ggagaccatg ttggcgaagg tgatcttcat ggtgacatag ctgttgaggg agatcttcgt
      841 gagctcggcg ttctcgatgg acatccgctt cacgggcgga ttgttctcca tgatccggga
      901 gtagtatccg gtgagcatat cacctgactt tttatcaaac tcgccgatga ggaggaagtc
      961 ggggttcagg aagtcgtgga tgacgctccc cagggcgatg aactcggggc tgtagcagag
     1021 gccgaagtcc ttcccgcatt tcttccccga cgcctcctcg aggaccggga ggaggccgaa
     1081 ccgggtcgag cccggtaaga cggtgctcgt cagcacgatg gtgtggtacc cgtccttctc
     1141 ccgcagggcc tcgccgatgg actggaaggc cgagatggca tatttgatgg agaaggcgcc
     1201 cctctcgtcg ctcggcgtgg gaacgatgac gaaggtgatg tcggagttct ggacggcatg
     1261 gcggtaatcc gccgtggctt ccaggttttt ccggtgacgc gtgatgtatt cggcgaggtc
     1321 ggtctccact accggcggtt ttccctggtt caggagctcc acggcccggg ggaccacgtc
     1381 caccccgatg accttcaggc cccggctcgc catggcggcg gccatgctcg cgccgagctt
     1441 cccgagaccg atgatggaaa cagtgtcccc ggtcatgacc tcatgcctcc tgagcatctt
     1501 cgggggcaac cgcatcctcg gccatccttc tcattcctcc acgccgcctg cccggggggg
     1561 taaaaaaccg gtcatccctg tcagcatctc atttctcctc atccctcatg tagctcgcga
     1621 tcccgagggc catccctgcc gccgccccga gggtcgcgac atcccccttc cggaggtgtt
     1681 cccggagtgc cgtgaggggg atgagctcga cccggatttc ctcgagatcg tcgggggtag
     1741 gatccccctc cctgacggca ttccgggcca aaaagacgtg ggccttcccg cacccccggt
     1801 tcccgtggac cgagaaggaa cccaggtgcc gccagccgga tgccgaaaat cccgtctcct
     1861 ccatgagttc gcggcgggcg gcctcgaggg gcgcctcccc ttccttcaca tatcccgcgg
     1921 ggagtcccag gttgatcctc tgcggcccgt gcttgtagtg ccagatcccc acgacctcgt
     1981 cgcggggtgt caccgcaaag accacggcaa agtccggctc ctcgatggta tagtaatcgt
     2041 cgatgacccg gccgtcctcc agccggacct tttccctgac gagcctcatc cacggtgcgg
     2101 cgtcggccac gcattcccgg tcaaggactt cccagcactt catacgagac gggcctggtc
     2161 aggtacaggg agcctggtaa atcctgctct cgcggagctc ctcgatggcc ttcttccggt
     2221 ccggtgcatg gtccagggta tcccggacca gggcctcgat ccaccggtac gtgatcccga
     2281 gccccttctc gaggcttatc tccggttccc agcgaaggac tttccggagc agggtgttgt
     2341 ccgagttccg gccccgcaca ccctgagggc cctcgatatg tccgatctca acctgcgtat
     2401 cggcgattcc cgcgatgatc cccacgagct cgttcatgtt caccatccgg tcggagccaa
     2461 ggttgagggg gtcatggaag tcagaagcca tgagcttcat aaggccgagg acacagtcgt
     2521 cgatgtagca gaaggaccgg gtctgttcgc cgtcacccca gatgtcgatg gagggatccc
     2581 cggaaagctt cgccaccgcg accttccggc agaaggccgc cggcgccttc tcccgtcccc
     2641 ccacccatga tcccttcggg ccgtaga
//
LOCUS       DATGDI010000011         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_263054, whole genome shotgun sequence.
ACCESSION   DATGDI010000011 DATGDI010000000
VERSION     DATGDI010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263054"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1298)
                     /locus_tag="VKO45_00555"
     CDS             complement(117..1298)
                     /locus_tag="VKO45_00555"
                     /EC_number="4.1.2.43"
                     /EC_number="4.2.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,6,7,8-tetrahydromethanopterin
                     hydro-lyase/3-hexulose-6-phosphate synthase"
                     /protein_id="HMA04398.1"
                     /translation="MYSIGEALLGDGAELAHIDLIVGDKEGPVGAAFANALSLLSVGH
                     TPLLAVIRPNLLTKPATLVIPKVTLKNVKQVNEMFGPVQAAVSKAVADSLEEGVFKDT
                     DIEQIVILCSAFLHPEAKDYNKIYRYNYGATKLALRRAFENFPDAKTLLYEKDRQAHI
                     IMGFKVTRLWDPPYLQVALDLVDMKQVARVLQSLPDNDHLIVEAGTPLIKKFGIGVIS
                     ELRKARPNSFIIADLKILDTGNLEARMAADASADAVVVSGLAPVQTLEKAITEAKKTG
                     IYSVMDMLNVPDPVKVIKSLAVKPDVVELHRAIDAEREDHAWGDIPALKKAAGGKLLV
                     ATAGGIRVPVVKAAVKAGADIIVVGRAITASRDIKHAAEEFLEQLQKEEIDQFRVMTD
                     F"
     gene            complement(1471..>2403)
                     /locus_tag="VKO45_00560"
     CDS             complement(1471..>2403)
                     /locus_tag="VKO45_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04399.1"
                     /translation="KPVYGDAAGTQLTALLRQHILIAVDIVNAVKAKNATAQAAAETA
                     WTANADQIATFLAGANPKWPKATLQELMYKHLSTTKDELVARYTMNYTADVAAWDAVY
                     NHILMMSDALSDGILAQHPEKFSGPAIYTQSQVDLRNGMRKLWTDHTVWTRLYIIESL
                     NDSPAATPAAARLLQNQVDIGNAIKPVYGDAAGTQLTALLREHILIAVDIIDAVKAKN
                     ATAQAAAETAWTANADQIATFLAGANPNWPLQTVKDLMHMHLSTTKDELVARYTMNYP
                     GDVTAWDAVYNHILTMSDALTDGIVKQFPAKFAS"
BASE COUNT          389 a          699 c          806 g          509 t
ORIGIN      
        1 atcagaacga aattagatga ttggttattc ggtaataatt tcacacggcc aaatacaatt
       61 tagtgcagat gttcttgaat tgatgtttta taattattta aaaaaatata taatccttaa
      121 aagtccgtca tcacccggaa ctggtcgatc tcctccttct ggagctgttc gaggaactcc
      181 tcggcggcgt gcttgatgtc ccgtgacgcc gtaatcgccc gtccgacgac gatgatgtcc
      241 gcgccggcct tcaccgccgc tttcacgacg gggacgcgga tcccgcctgc cgtcgcgacg
      301 aggagcttcc cgccggcggc cttcttcagg gccgggatgt ccccccaggc atggtcctcc
      361 cgctcggcgt cgatggcccg gtggagctcc acgacgtcgg gcttcaccgc gagggacttg
      421 atcaccttca ccgggtcggg cacgttgagc atgtccatca cagaatagat gccggtcttc
      481 tttgcctcgg tgatggcctt ctcgagggtc tggacgggtg cgaggccgga gaccaccacg
      541 gcgtcggcgg atgcgtccgc ggccatccgg gcctcgaggt tcccggtgtc gaggatcttc
      601 aggtccgcga tgatgaagga gttgggccgg gccttgcgga gctcgctgat gaccccgatc
      661 ccgaacttct tgatgagtgg ggtccccgcc tcgacgatga ggtggtcgtt gtcgggcagg
      721 gactggagga cccgggcgac ctgcttcatg tccacgaggt cgagggccac ctggaggtag
      781 ggcgggtccc agagcctcgt caccttgaag cccatgatga tatgggcctg ccggtccttc
      841 tcgtagagga gcgtctttgc gtcggggaag ttctcgaagg cccggcggag ggcgagcttc
      901 gtggccccgt agttgtaccg gtagatcttg ttgtagtcct tggcctcggg gtggaggaag
      961 gccgagcaga ggatgacgat ctgctcgatg tctgtatcct tgaagacccc ttcctcgagg
     1021 gagtcggcca cggccttgct caccgccgcc tggaccgggc cgaacatctc gttcacctgc
     1081 ttcacgttct tcagggtgac cttcgggatg accagggtcg ccggcttcgt gaggaggttc
     1141 ggccggatca ccgcgaggag gggtgtgtgc cccaccgaga gcagggagag ggcgttggcg
     1201 aacgccgccc ccaccggccc ttccttgtcc ccgacgatga ggtcgatgtg ggcgagctcc
     1261 gcgccatccc ccaggagtgc ttctccgatg ctgtacatga cgtgctccgt tccttgagaa
     1321 aattatagtg ccccggagta taaaaaaggg gggagtctgt ccccgggcgg gggatccgga
     1381 ccatcgcccg aggccagtcc tgcggggagg cgattcggga atccggaatg cgccgctccc
     1441 gtccacaaaa agaatgaaaa aaagggatgc tcagctcgcg aacttcgcgg ggaactgctt
     1501 cacgatcccg tcggtgaggg catccgacat ggtgaggatg tggttgtaca ccgcgtccca
     1561 cgcggtgacg tcgcccgggt agttcatcgt gtaccgggcc acgagctcgt ccttcgtcgt
     1621 cgagaggtgc atatgcatga ggtctttcac ggtctggagc ggccagttgg ggtttgcccc
     1681 ggcgaggaag gtcgcgatct gatccgcgtt cgccgtccat gccgtctcgg ccgccgcctg
     1741 ggcggtggcg ttcttggcct tcaccgcatc gatgatgtcc acggcgatga ggatgtgctc
     1801 cctgagaagc gcggtgagct gggtcccggc cgcgtcaccg tagaccggct tgatggcgtt
     1861 cccgatgtcc acctggttct ggaggaggcg tgccgccgcc ggtgtggccg ccggcgagtc
     1921 attcagggac tcgatgatgt agagcctcgt ccagacggtg tgatccgtcc agagcttcct
     1981 catgccgttc cggaggtcga cctgggactg ggtatagatg gcgggcccgg agaacttctc
     2041 ggggtgctgc gcgaggatcc cgtcggagag ggcatcggac atcatgagga tgtggttgta
     2101 cacggcgtcc catgccgcga catcggcggt gtagttcatc gtgtaccggg cgacgagttc
     2161 atccttggtc gtcgagaggt gcttgtacat gagttcctga agggtcgcct tgggccattt
     2221 ggggtttgcc ccggcaagga aggtcgcgat ctgatccgcg ttcgccgtcc atgccgtctc
     2281 ggccgccgcc tgggcggtgg cgttcttggc cttcacggcg ttgacgatgt ccaccgcgat
     2341 gaggatgtgc tgcctgagga gcgccgtgag ctgggtcccg gccgcgtccc cgtagaccgg
     2401 ctt
//
LOCUS       DATGDI010000012         3673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_117735, whole genome shotgun sequence.
ACCESSION   DATGDI010000012 DATGDI010000000
VERSION     DATGDI010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117735"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..686)
                     /locus_tag="VKO45_00565"
     CDS             complement(117..686)
                     /locus_tag="VKO45_00565"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMA04400.1"
                     /translation="MADMLTAILVTLPPVVFLIILFGGGFLSLRKHIDQDGNSPIHRT
                     LFYTSKWSVVILWIGMMVQVWGIQVSFVKVPAELTWVSLFLWFSGFALLYYGRFKLGG
                     SFRLGTPKESTNLTTDGLFRFSRNPMYLGLYSTIVASIVYTLNPLVLLLGIFVMVIHH
                     KIVLGEEAYMQKVFGQAYLDYCRRVRRYI"
     gene            complement(764..836)
                     /locus_tag="VKO45_00570"
     tRNA            complement(764..836)
                     /locus_tag="VKO45_00570"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(800..802),aa:Asp,seq:gtc)
     gene            complement(861..965)
                     /locus_tag="VKO45_00575"
     tRNA            complement(join(861..896,928..965))
                     /locus_tag="VKO45_00575"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(929..931),aa:Tyr,seq:gta)
     gene            1098..2615
                     /locus_tag="VKO45_00580"
     CDS             1098..2615
                     /locus_tag="VKO45_00580"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HMA04401.1"
                     /translation="MVFFTPGKIREKITVFDTTLRDGEQTPGISFTLPQKQEIARQLS
                     EIGVHVIEAGFPASSESEHETVKAIAGMGLEAQVCGLARMTKGDVDACLDCGVDMVHV
                     FIPTSDVQRIHTIKKSREEVLEVSEEVVSYARERCDQVLYSAMDATRTDWDYLVQVLQ
                     VAVQAGATVLNIPDTVGVITPSAMKMLIGRIVAEVDCPIDVHCHNDFGLAVANTLAAV
                     EAGASQVQVTVNGLGERAGNADLAETVMALESIYGVSTGIHTQRIVETSRLVSRCSGI
                     TIIPTQPIVGENAFSHESGIHSHGVIARSDTFEPGIMTPEMVGHRRRLKLGKHVGRHA
                     VRQMLADVHLAPEEPQLDEIVTRVKDMSGKGRLVTDADLYEIATTVMGVVSNGKTIDL
                     QEIAVITGNHVIPTASLKAMVDGVEHISSSVGVGPVDAALKAIMGILPTRVTLKEFKI
                     EAISGGSEAIGHVRVALEDEKGHIFDASGTGDDIVIASVEAMVNAINLLHRVRKG"
     gene            complement(2666..3034)
                     /locus_tag="VKO45_00585"
     CDS             complement(2666..3034)
                     /locus_tag="VKO45_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HMA04402.1"
                     /translation="MENIPPKVQNQLAMLQQLQQQLQTVAAQKTQYEMAIRESNRAQE
                     ELKDVPEDAEIFVNVGTVMMQQKKAKVVASLTEKSESLELRVKSLEKQEKAMQARFEQ
                     LSTDIKGSLDRKRPAVPTAE"
     gene            3216..>3673
                     /locus_tag="VKO45_00590"
     CDS             3216..>3673
                     /locus_tag="VKO45_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA04403.1"
                     /translation="MATRSRRCGKAKGARILMIPASRTDVAERRSLLREIDQLMGRIR
                     KGGKEVDEGLVRAKEAIEDEKNHLLIVRSRWGDLLGSLSYTFPDAGSIRVDYIGLSVH
                     RCGLGTRLMEAVAAIAVKGNRRILLTAEKDAKGFFRAMGMVLREEYPDGNG"
BASE COUNT          764 a         1081 c         1118 g          710 t
ORIGIN      
        1 cttgtttgcc ttcatagagg tttctccatt tcatggccta ggatggaatg tttcgagact
       61 atcataagcc agcccaaaat ctctcccctg aacgaaaaga gcgataacat ttttagttag
      121 atataccgtc ttacccgacg acaataatcc aaatatgcct gcccgaacac cttctgcata
      181 taggcttcct cccccaggac gatcttatgg tggatcacca tgacaaaaat ccccagtaaa
      241 agcaccaagg gatttagggt ataaacgatc gatgcaacga tggttgaata taatcccaga
      301 tacatcggat ttctgctgaa tctgaatagt ccgtccgtcg taagatttgt gctttccttg
      361 ggagtcccca gacggaaaga tccgccgagc ttgaatcgcc cataatagag caacgcgaat
      421 ccagaaaacc aaagaaacag agagacccag gtgagttccg caggaacctt gacgaaggag
      481 acctggatgc cccagacctg caccatcata ccaatccaca ggataacgac tgaccattta
      541 ctcgtgtaga ataaagtcct atgaatcggg gaattcccat cctgatcgat atgtttacgc
      601 agggatagaa atcccccacc gaataagata atcaggaata ccacaggagg cagtgttaca
      661 aggatggctg tgagcatgtc tgccatattc actgtaacat atcccgtcgg tctccctatt
      721 aaagtgtatt atcgtgtcta ggaggatttc aggaaaagat cgacgcccag gtcggaattc
      781 gaatccgagt cgcgggcgtg acaggcccgc atgataggcc gctacactac ctgggcactc
      841 gatggcactt gtgtgggtta tcccgcctcc cggattcgaa ccggggacat cgcggtagct
      901 gcgcagttac ctacacggca aaccagacta cagccgcgca ctctaccagg ctgagttaag
      961 gcgggtaagt gcccttgagt atagggaaat ttccgtgata aacatatcgt tccctctggc
     1021 gggccgtgtt tcacccgaat ttctttatag gatgaatacc tttggtacag gtatgccaga
     1081 acagactgaa gcggggtatt gtcttcttca cccccggtaa gattcgcgag aagatcactg
     1141 ttttcgacac caccctccgg gatggcgagc agactcctgg catctccttc acgctccccc
     1201 agaagcagga gatagcccgc cagctctccg agatcggggt ccatgtcatc gaggccggct
     1261 tccccgcctc ttcggagtcc gagcacgaga cggtgaaggc catcgcgggg atgggcctcg
     1321 aggcgcaggt ctgcggcctc gcccggatga cgaaggggga cgtggacgcc tgcctggact
     1381 gcggggtgga catggtgcat gtcttcatcc cgacctcgga tgtccagcgg atccacacca
     1441 tcaaaaaatc ccgcgaggag gtcctcgagg tctccgagga ggtcgtctcc tatgcccggg
     1501 agcgctgcga ccaggtcctc tactcggcca tggacgcgac gaggaccgac tgggattacc
     1561 tggtccaggt cctccaggtc gcggtccagg ccggtgcgac ggtgcttaac atcccggaca
     1621 ccgtgggggt catcaccccc tcggcgatga agatgctcat cgggaggatc gtggccgagg
     1681 tggactgccc catcgacgtc cactgccaca acgacttcgg cctcgccgtg gcgaacaccc
     1741 tcgccgccgt cgaggcgggg gcatcccagg tccaggtgac cgtgaacggc ctcggcgagc
     1801 gggcggggaa cgccgacctc gccgagacgg tcatggccct cgagtccatc tacggggttt
     1861 cgacgggcat ccacacccag aggatcgtgg agacctcacg gctcgtctcg cgctgctcgg
     1921 ggatcaccat catccccacc cagcccatcg tgggggagaa tgccttctct cacgagagcg
     1981 gtatccattc ccacggggtc atcgcccgct ccgacacctt cgagccgggc atcatgaccc
     2041 cggagatggt gggccaccgg cgccggctga agctcgggaa acatgtggga aggcacgcgg
     2101 tgaggcagat gctcgccgac gtccacctgg cgcccgagga accccagctc gacgagatcg
     2161 tcacccgggt gaaggacatg tccgggaagg ggaggctcgt caccgacgcg gacctgtacg
     2221 agatcgcgac cacggtcatg ggggtggtct ccaacgggaa gaccatcgac ctccaggaga
     2281 tagcggtcat caccgggaac catgtcatcc ccacggcctc cctgaaggcc atggtggacg
     2341 gggtcgagca catctcctcg agcgtcgggg taggcccggt ggatgccgcc ctgaaggcga
     2401 tcatggggat ccttcccacg cgggtcaccc tgaaggagtt caagatcgag gccatctcgg
     2461 ggggctcgga ggccatcggc catgtccggg tggccctcga ggacgagaag gggcacatct
     2521 tcgacgcgag cgggaccggt gacgacatcg tcatcgcctc ggtggaggcc atggtgaatg
     2581 ccatcaacct gctccaccgg gtcaggaagg gatgagggga agggcccctc cctgtttttc
     2641 cagaactcgt acgataaaaa agtccttatt ccgccgttgg cactgccggc ctcttccggt
     2701 ccagggaccc cttgatgtcg gtggagagct gctcgaacct tgcctgcatg gccttctcct
     2761 gcttctcgag ggacttcacc cggagctcca ggctctccga cttctcggtg agggaggcga
     2821 cgaccttcgc cttcttctgc tgcatcatca ccgtgccgac attcacgaag atctcggcat
     2881 cctcggggac gtccttcagc tcctcctggg cacggttcga ctcccggatg gccatctcgt
     2941 actgggtctt ctgggccgcc acggtctgga gttgctgctg gagctgctgg agcatggcga
     3001 gctggttctg gaccttgggt ggaatattct ccatgaatcc gattcctccc tctccggtac
     3061 gcacccatgc ccattatacg ttttggactt tctggggttc ctgtccgggg gcgacaccgt
     3121 gttgattata tatccgggag ggcaccggtg gaggggaccg gatcggtgtc cctgcgggga
     3181 ttcgcgtggc aaacgccagg ggtgagggcc gggctgtggc taccaggtcc cggcggtgcg
     3241 ggaaggcgaa gggcgcgagg atcctcatga tccccgcctc gaggacggac gtggccgagc
     3301 gaaggagcct cctccgggag atcgatcagc tgatgggaag gatacggaag ggcgggaagg
     3361 aggtggacga gggcctcgtc agggcaaagg aggccatcga ggatgagaag aaccacctgc
     3421 tcatcgtgcg gtcccggtgg ggagatctcc tcgggagcct ctcgtacacc ttccccgacg
     3481 ccggcagcat acgggttgat tacatcggcc tctcggtcca ccggtgcggt ctcggcaccc
     3541 ggctcatgga ggcggtcgcg gccatagccg tcaagggaaa ccggaggatc ctgctcacgg
     3601 cggagaagga tgcgaagggc ttcttcagag ccatggggat ggtgctgcgg gaggagtacc
     3661 cggacggaaa cgg
//
LOCUS       DATGDI010000013         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_350434, whole genome shotgun sequence.
ACCESSION   DATGDI010000013 DATGDI010000000
VERSION     DATGDI010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350434"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VKO45_00595"
     CDS             <1..387
                     /locus_tag="VKO45_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HMA04404.1"
                     /translation="DDETAAEILGKNAYRFKIKSFVIGSVFAGLAGVLWAHYATFISP
                     GDFTLTETILVLLIVVMGGKGTFLGPVVGAVVLIFFQESVRFLRLPPEWTRLLGPLQQ
                     MIFGLLLIVLMVFRPEGIIREKGGER"
     gene            384..1130
                     /locus_tag="VKO45_00600"
     CDS             384..1130
                     /locus_tag="VKO45_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA04405.1"
                     /translation="MIEIRNVTKAFGGTRAVSDCSISLDGLTISGLIGPNGSGKTTLF
                     NLITGLLKPDSGEIHFLGSRIDGRLPHDMAALGLIRTFQLSRVFPRMTVLENMLLAPQ
                     GQTGERFLSLWLSQPKIRREESENRERALELLDLLGLTRLHDQFAENLSYGQQKLLEL
                     GRALMAKPKMILLDEPTAGINPTLIRTMIEMILKMRERGQTFFIIEHNMDVVIEVCEK
                     VFVLDHGEKIAEGRQEEIQKDPHVIVAYLG"
     gene            1127..>1509
                     /locus_tag="VKO45_00605"
     CDS             1127..>1509
                     /locus_tag="VKO45_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA04406.1"
                     /translation="MMRLEAADIYAGYGKIEVLHGATLRAAAGEIVCIIGPNGSGKST
                     LLKTVFGLLKPTKGTVSFDGKDITRLEPEQKVHLGIAFIPQGRNVFPSLTVRENLEMG
                     GTALDDEKAVQRAIEEALASYPLLK"
BASE COUNT          304 a          472 c          452 g          281 t
ORIGIN      
        1 gacgacgaaa cggcggcgga gatcctcggc aaaaatgcct atcgcttcaa gatcaagtcc
       61 ttcgtcatcg gctccgtctt tgcgggcctc gcaggagtgc tctgggccca ctacgcgacc
      121 ttcatcagcc ccggcgattt cacgctgacc gagacgatcc tcgtgctgct cattgtcgtc
      181 atgggcggaa agggcacctt cctgggaccc gttgtggggg ccgtggtcct catcttcttc
      241 caggagtcgg tccgattcct gcggctcccg cccgagtgga caaggctgct gggtccgctg
      301 cagcagatga tcttcgggct gctgctcatc gtcctcatgg tctttcgccc cgagggtatc
      361 atccgcgaaa aagggggaga gcgttgatcg agatccgaaa cgtgacgaag gccttcggcg
      421 gcacgcgcgc cgtctccgac tgctcgatca gcctcgacgg cctgacgatc tccggtctca
      481 tcggtcccaa cgggagcggt aaaacgaccc tcttcaacct catcacgggc cttctcaaac
      541 ccgacagcgg ggagatccac ttcctgggta gccggatcga cggccgcctg ccccatgaca
      601 tggcggccct gggcctcatc cgcacctttc agctctcgag agtcttcccc aggatgacgg
      661 tactggagaa catgctgctt gccccccagg gccagacggg ggagcgcttt ctgtccctgt
      721 ggttgagcca gccgaaaatt cgcagggagg aatcggaaaa ccgggagcgg gccctcgagc
      781 ttctcgatct tctgggtctc acccgcctcc atgatcagtt cgccgagaac ctctcctacg
      841 ggcagcagaa gctcctggag ctgggccggg ccctcatggc gaaaccaaag atgatccttc
      901 tggacgaacc cacggcgggg atcaacccca cgctgatccg gacgatgatc gagatgattc
      961 tcaagatgag ggagcggggc cagacatttt tcatcatcga gcacaatatg gacgtggtca
     1021 tcgaggtctg cgagaaggtt ttcgtcctcg accacggaga gaagattgcg gaaggacggc
     1081 aggaagagat ccagaaggat ccccacgtga tcgtggccta cctgggatga tgagactgga
     1141 agctgcagac atctatgccg gctacggcaa gatcgaggtt cttcatggag caaccctccg
     1201 tgcagccgca ggggagatcg tctgcatcat cggtcccaac ggctcgggaa aatcgaccct
     1261 gctgaaaacg gtcttcggcc ttctgaagcc gacgaagggc acggtgagct tcgacgggaa
     1321 ggacatcacc cggctcgagc ccgagcagaa ggtacacctc gggatcgcct tcatccccca
     1381 gggccgcaat gtcttcccgt cgctcacggt ccgcgagaac cttgagatgg gcggcacggc
     1441 cctcgacgat gaaaaggccg tccagcgcgc gatcgaggag gccctcgcat cgtaccctct
     1501 cctcaagaa
//
LOCUS       DATGDI010000014         4148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_350480, whole genome shotgun sequence.
ACCESSION   DATGDI010000014 DATGDI010000000
VERSION     DATGDI010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350480"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VKO45_00610"
     CDS             <1..412
                     /locus_tag="VKO45_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04407.1"
                     /translation="DAEPRQGMIDARSVSLEKYLELGRGERVVVDSLSTVILKYGIDT
                     AMKFLSICVKDTEKRQSNYLFLVYRGIHTPVEEMRINRLADTVLEFREEYEGIEIQHS
                     LIVHKIRGMDVPNRAIPLLIKESGLEISTTSRVV"
     gene            complement(543..1490)
                     /locus_tag="VKO45_00615"
     CDS             complement(543..1490)
                     /locus_tag="VKO45_00615"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HMA04408.1"
                     /translation="MILIHKVLPTTVVGSYPVAKSPSFRSLLDPLKSAVETAVTDQLT
                     AGIDIISDGQVRGDMISVFVSRLPGIRGQQVVGKVLPPEKPITIPDTRYAVSRHPWVK
                     GILTGPTTLAHALQISTPMYRDRADLTLDLAQALAYEARALQDAGVTLIQIDEPIFST
                     GAADLATGAQALQVLTSLLQKPVILHVCGVLAGVIDDVLKMRVAGFDFEFAGNEVNLQ
                     LLSGKDLGDRFLGFGCVNSSDPAVERVPVIKKRIQAGIEAFGPERLLIDPDCGLRMLT
                     REVAFQKLKAMVDATREARVEYTDATPRDSSWRSRDRSF"
     gene            complement(1487..2776)
                     /gene="aspS"
                     /locus_tag="VKO45_00620"
     CDS             complement(1487..2776)
                     /gene="aspS"
                     /locus_tag="VKO45_00620"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050560 - aspartate-tRNA(Asn) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HMA04409.1"
                     /translation="MASPIASITPGSGRAEIAGWVHEVRDLGGLTFCLVRDRTGIVQV
                     TVNRKKAPAPVIEALSNLSRESVVRVGGTVKAIEKAPGGREIVPDRFEIIAPAGSPLP
                     LDVVEKVPAELDTRLDSRFLDLRRPRVAAVFLIRAVVMGTIWEFLSSRGFVNIATPKL
                     VAAATEGGTDLFPVAYFEKEAFLNQSPQLYKQMMMAAGFERVFEIGPIFRAEEHNTTK
                     HLNEATSIDVEASFCDDREVMDLLEDLVIAVYERVRADCADPLSWIGVDLEIPRKPFP
                     RLPYRDAIGIAARRTGEPLKFGDDFGTTAEKAVGDEMGVHYFIVDWPTAIKPYYAMPC
                     SDNPELSRSFDLMHPRMELASGSQRIHQHDLLVHQIRSKGLSADSFEFYLRPFRYGMP
                     PHAGWGLGAERLVMTMLGLPNIREAVIFPRDRHRLVP"
     gene            2998..3531
                     /gene="cbiT"
                     /locus_tag="VKO45_00625"
     CDS             2998..3531
                     /gene="cbiT"
                     /locus_tag="VKO45_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HMA04410.1"
                     /translation="MKPTAGSTAEEVLAVALRKLALRPGDIFADIGCGSGRVTLAASP
                     LVREVYAIDRRPEAVEYAREQARVAGAVNIRFLEGEAPRVLGDLPAPDRAFIGGSRHM
                     SDVIRALASGGAARMAVSCVRIETLAEAVSAMRELGIFREAILLQVARSRDLAGGTMF
                     VPGNPVYLVIGGTEPCS"
     gene            3522..4124
                     /locus_tag="VKO45_00630"
     CDS             3522..4124
                     /locus_tag="VKO45_00630"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HMA04411.1"
                     /translation="MLIGLGLGPGDPELLTLRAVRLLREADAVFVPGGIAQKLVAPYR
                     DAEVLAFPMTGDEAAIRRAIGESAERIAKVAVNGVAVFALLGDPNIYSTYARLLGAIR
                     ERHPEIPCKTVPGVSAITAFASVAGITLSDGFSVSDGRGERGRILLKVRRPREAAERL
                     AREGFREFVLVERMFLDGERVYRGELPEESTYFSILYAGR"
BASE COUNT          684 a         1325 c         1427 g          712 t
ORIGIN      
        1 cgatgccgag cccaggcagg gaatgatcga cgcccgcagc gtctccctcg agaaatacct
       61 ggagctcggc cggggagagc gggtggttgt ggactccctc tcgacggtga tcttgaagta
      121 cgggatcgac accgcgatga agttcctttc tatctgcgtg aaggacaccg agaaacgaca
      181 gtccaactac ctcttcctcg tgtatagggg gatccacacc ccggtcgagg aaatgcggat
      241 caaccggctc gccgatacgg tgcttgagtt cagggaggag tacgagggaa tcgagatcca
      301 gcactccctc atcgtccaca agatccgggg gatggatgtc ccgaaccggg ccatccccct
      361 cctcatcaaa gagagtggcc tcgagatatc cacgacgagt cgcgtggtgt agcgaggttt
      421 catcagaggg agggtccgca ggacccgacc tcgagcgacg aagtcgcgaa gaacccgacc
      481 tggagcaacc cgccgccggg cggggtgcga cggagcgccc ggtccgggac agccccctct
      541 cattagaaag accggtcccg ggatctccac gacgaatcgc gtggcgtagc gtcggtgtac
      601 tcgacccggg cctcccgggt cgcgtccacc attgccttta atttctggaa ggcgacctcc
      661 cgggtgagca tccgcaggcc gcagtccggg tcgatgagga gccgctcggg gccgaaggcc
      721 tcgatccccg cctggatccg tttcttgatc accgggaccc tctccaccgc ggggtcgctc
      781 gagttcacgc acccgaagcc gaggaaccgg tccccgagat ccttccctga gaggagctgg
      841 aggttcacct cgttccccgc gaactcgaag tcaaagccgg cgacccgcat cttcagtaca
      901 tcgtcgatga ccccggcgag gaccccgcag acgtggagga tgaccggctt ctggaggagg
      961 gaggtgagga cctggagggc ctgcgccccg gtggcgaggt ccgccgcccc cgtggagaag
     1021 atgggttcgt cgatctggat gagggtgacc ccggcgtcct ggagggcccg ggcctcgtag
     1081 gcgagggcct gggcgaggtc cagggtgagg tccgccctgt cccggtacat gggcgtcgag
     1141 atctggaggg catgggcgag ggtcgtgggc ccggtcagga tccccttcac ccacgggtgg
     1201 cgggagacgg cgtagcgggt gtccggtatc gtgatgggct tctcgggggg gagaaccttc
     1261 cccacgacct gctgcccccg gatccccggg agcctcgaga caaagacgga gatcatgtcc
     1321 ccgcggacct ggccgtcgga gataatgtcg atccccgcgg tgagctggtc ggtgacagcg
     1381 gtctccaccg ccgacttcag ggggtcgagg agggaccgga aggaggggct cttcgccacg
     1441 gggtagctgc caacgaccgt ggtcgggagg accttgtgga tgaggatcat ggcacgagcc
     1501 ggtggcggtc ccgggggaag atcaccgcct ccctgatgtt cggaagcccg agcatggtca
     1561 tgaccaggcg ctctgcgccg aggccccagc ccgcgtgggg gggcatcccg taccggaagg
     1621 gccggaggta gaactcgaag gagtcggccg agagcccctt ggaccggatc tggtggacga
     1681 ggaggtcgtg ctggtggatc cgctgcgagc cgctcgcgag ctccatccgg gggtgcatga
     1741 ggtcaaagga ccgggagagc tcggggttgt ccgagcacgg catggcatag tagggcttga
     1801 tggccgtggg ccagtccacg atgaagtagt ggacccccat ctcgtccccc acggccttct
     1861 cggcggtggt cccgaagtcg tcgccgaact tcaggggctc ccccgtcctc cgggccgcga
     1921 tcccgatggc gtcccggtaa gggagccggg ggaaaggttt ccgggggatt tccagatcca
     1981 ccccgatcca ggagagggga tcggcacagt ccgccctgac ccgctcgtag accgcgatga
     2041 cgagatcctc caggagatcc atcacctccc ggtcatcgca gaaggaggcc tccacgtcga
     2101 tggaggtggc ctcgttcagg tgcttcgtcg tgttgtgctc ctcggcccgg aagatggggc
     2161 cgatctcgaa gacccgttcg aagcctgcgg ccatcatcat ctgcttgtag agctgggggc
     2221 tctggttcag gaaggcctcc ttctcgaagt aggccaccgg gaagaggtcg gtccccccct
     2281 cggtcgcggc agccacgagc ttcggggtcg cgatgttcac gaacccccgg gaggagagga
     2341 actcccagat cgtccccatt acgacggccc ggatgaggaa gaccgcggcc acccggggcc
     2401 tccgcaggtc gaggaaccgg gagtcgagcc gggtgtcgag ctcggccggc accttctcca
     2461 ccacgtcgag ggggaggggg gatccggccg gggcgatgat ctcgaaccgg tcggggacga
     2521 tctcccggcc gccgggggcc ttttcgatgg ccttcaccgt tcccccgacc cgcaccaccg
     2581 attcccggga gaggttggag agtgcctcga tcaccggcgc cggcgccttc ttccggttca
     2641 ccgtgacctg gacgatgccg gtccggtccc ggacgaggca gaaggtgagg ccccccaggt
     2701 cgcgcacctc gtggacccag ccggcgatct ccgcgcgccc gctcccgggc gtgatggatg
     2761 cgatggggct tgccatacca gatacctcgt atatatcccc tgcaggggta aaaggtctat
     2821 ggattcggac tacgggtgaa cgacggagct ccttcggttc cccccttgtc gcggtttatc
     2881 ttttcatttc tgaattggtc gaagatcggc tgatgccgat cttctccatt accggccgcc
     2941 ctgcgggcgc ccggtacaca ataaaaatat accttaacgc aattatactt gagtggaatg
     3001 aagcccaccg cgggttccac cgccgaagag gtccttgccg tggccctccg gaagctcgcc
     3061 ctccggccgg gcgatatctt cgccgacatc gggtgcggtt cgggcagggt gactctcgct
     3121 gcctcccccc tcgtacggga agtctatgcc atcgaccgcc ggccggaggc cgtcgagtac
     3181 gcgagggaac aggcgcgcgt ggcaggagca gtgaatatcc gcttcctgga gggagaggcc
     3241 ccccgggtcc tcggggatct tcccgcccct gaccgggcct tcatcggcgg ttcccggcat
     3301 atgtccgatg tcatccgggc ccttgcctcg ggtggggccg cgagaatggc cgtctcctgc
     3361 gttcggatcg agaccctggc ggaggcggtc tcggccatgc gggagctcgg catcttccgg
     3421 gaggcgatcc tcctccaggt cgcccggtcc cgtgaccttg ccggcggcac catgtttgtc
     3481 cccgggaacc cggtgtacct cgttatcggg gggacggaac catgctcata ggccttggcc
     3541 ttggccccgg cgatcccgag ctcctcaccc tgcgggcggt ccggctcctt cgcgaggcag
     3601 atgccgtctt cgttcccggg ggaatcgcgc aaaaactcgt ggcaccctac cgggacgctg
     3661 aggtgctcgc ctttcccatg accggcgacg aggcggcgat ccgccgggcc atcggggaat
     3721 cggcggagag gatcgccaag gtggcggtaa acggggtcgc ggtctttgcc ctcctcgggg
     3781 accccaatat ctactccacg tacgcccggc ttctcggggc gatccgggag aggcacccgg
     3841 agatcccgtg taaaacggtc cccggcgtct ccgccatcac cgcctttgcc tcggtcgcgg
     3901 ggatcaccct ctcggacggc ttttcggtct cggacgggag gggcgagagg gggcggatcc
     3961 tcctcaaggt ccgccggccg agagaggcgg cggaacggct cgcccgggag ggcttccggg
     4021 aattcgtgct cgtggaacgg atgttcctcg acggtgagcg ggtgtaccgc ggggagctcc
     4081 ccgaggagag cacgtacttc tccatcctct atgcggggcg ctaagatgga gccggtctat
     4141 ttcgtcgg
//
LOCUS       DATGDI010000015         1766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_205086, whole genome shotgun sequence.
ACCESSION   DATGDI010000015 DATGDI010000000
VERSION     DATGDI010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205086"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..>1766
                     /locus_tag="VKO45_00635"
     CDS             259..>1766
                     /locus_tag="VKO45_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMA04412.1"
                     /translation="MKKDESRTKRDLIAEVEELRGLIARHRETEARLRETEELYRNLA
                     DSSPVGLYLLVQGKVHFLNAQFERSTGYRADELIGQDALFMVHPEDRETVRRDAIEML
                     KGKRTSPYEFRTITRDGRTRWILGTVASIPFWGQRAVLGNHMDISEQKESRRRLEEME
                     TLEASILDALPVAVIGLRERQIISANRAVEAIFGWKPEEIIGRNTRFLYRSDDEYEEI
                     GRHFYPVLERQRIFSEEFPCRHRDGHDILCFVSTSRIGANLEEKRIVATYEDITERKR
                     AEAALKGSEERLRAILEGSPAPTFVIDRRHRVIGWNKALEELTGVRSDAVIDTDEHSK
                     ILYGEKRPTVADLLVDGAADLTAEIRRWYGGRYRRSELLEEAYEATGPFSFPGKEARW
                     LHFTSAAVRDARGEMIGAIETMTDISPLKRAEDDLRENVERLRKVMTGVIRAIDVIVE
                     TRDPYTAGHQHEVARLATAIATEMGLPADTVEAIYVAASIHDIGKIYVPAEIL"
BASE COUNT          405 a          513 c          553 g          295 t
ORIGIN      
        1 atgtacggcg ccaattcctt gtaaccgtac ggcagttttg gtaaagaata gaaatggggg
       61 ttttccatgg agtcctcctt gtttttgagt tgattccgat gaatgattct ggccggtaac
      121 aaaaacggta tacgcaggaa aggcccatcg agaatgagac cctgtgaact tctgagacgt
      181 atcaaaaatt agtttacatg attcattgag agaaaaatag ttcaaaaatt caaggccgaa
      241 tctcccggaa cggacacaat gaaaaaagac gaaagccgga ccaagcggga tctcatcgcc
      301 gaggtggagg aacttcgcgg gctgatcgcc cgtcaccggg aaacggaagc ccggttgagg
      361 gagacggagg agctgtaccg caacctcgca gacagctccc ccgtgggcct ttacctgctc
      421 gtgcagggga aggtccattt tctcaatgcc cagttcgaga gaagcacggg atatcgcgcc
      481 gacgagctca tcgggcagga tgccctcttc atggtccacc ctgaagaccg tgaaacggtc
      541 cgcagggacg ccatcgagat gttgaagggg aagcggacga gcccctatga gttccggacg
      601 atcaccaggg acggccggac ccggtggatc ctgggaaccg tggcatcgat ccccttctgg
      661 ggccagcgcg ccgttctcgg aaatcacatg gacatctcgg agcagaagga gtcccggcgc
      721 aggctcgagg agatggagac cctcgaggcc tccatcctcg atgccctgcc cgtggctgtc
      781 atcgggcttc gggagcggca gatcatctct gcaaaccgcg ccgtggaggc cattttcggc
      841 tggaagcccg aagaaatcat cgggcgcaat acgcggtttc tctaccgcag cgacgacgag
      901 tacgaggaga tcggccggca tttctacccc gtcctggagc ggcaacggat cttcagcgag
      961 gagttcccct gccggcacag ggacggccat gacatcctct gctttgtcag cacgtcgcgc
     1021 atcggggcca acctcgagga aaagcgcatc gtcgccacct acgaggacat caccgagcgc
     1081 aagcgcgctg aggcagccct gaagggaagc gaggagaggc tgcgggccat tctggagggc
     1141 tcgcccgcgc ccaccttcgt catcgaccgc aggcaccggg tcatcggatg gaacaaggcg
     1201 ctcgaggaat tgaccggagt ccgaagcgat gccgtcatcg acaccgatga gcactcaaag
     1261 atcctctatg gcgagaagcg ccctaccgtc gccgatctgc tggtcgacgg ggcggcggac
     1321 ctgacggccg agatccggag atggtacggc gggcgctacc ggcgctcgga gcttttggag
     1381 gaggcctacg aggcaacggg cccgttcagc ttcccgggca aggaggcgcg ctggctccac
     1441 ttcacctcgg ccgccgtacg ggacgccaga ggggagatga tcggcgccat cgaaacgatg
     1501 accgacatct cgccactcaa gagggccgag gatgatctga gagaaaatgt cgagagactc
     1561 aggaaggtga tgacaggcgt catccgggcc atcgacgtga tcgtcgagac ccgcgaccct
     1621 tacacggcag ggcatcagca cgaggtggca agactcgcca cagccatcgc aacggagatg
     1681 ggcctccccg cagacacggt cgaggcgatc tacgtggccg cctcgatcca cgacatcggg
     1741 aaaatctacg tacccgccga gattct
//
LOCUS       DATGDI010000016        13263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_379684, whole genome shotgun sequence.
ACCESSION   DATGDI010000016 DATGDI010000000
VERSION     DATGDI010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 13263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13263
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379684"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1240)
                     /gene="rpoA2"
                     /locus_tag="VKO45_00640"
     CDS             complement(77..1240)
                     /gene="rpoA2"
                     /locus_tag="VKO45_00640"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A''"
                     /protein_id="HMA04413.1"
                     /translation="MKESMEKRIDAAELPDRTREEMKKFLADRSFTDKQFTKILDRIV
                     REYQNTRIEACEAVGIVAAQSIGEPGTQMTMRTFHYAGVAEINVTLGLPRLIEIMDAR
                     REPSTPTMTIHLKPEYASDRDRAREVSWQVEAAPLAEFGDITIDMEKMLVLVQLNTAV
                     TDKRKIGVDRIMELAPKKIKERRHYRDFECEVEEEAARLLFLPKDRESYQNLFQLAEH
                     VRNVIVQGIDDIERVVVRKEEGEYILYTEGSNLSDVFEVAGVDTTRTRTNNISEIADV
                     LGIEAARNSIIYEALSTLAEQGILVDVRHIMLVADMMCMDGEVKQIGRHGIAGEKESV
                     LSRAAFEVTVNHLLDAAVANEVDELNGVTENIIVGQPIQLGTGDVKLIAKPMH"
     gene            complement(1237..3882)
                     /locus_tag="VKO45_00645"
     CDS             complement(1237..3882)
                     /locus_tag="VKO45_00645"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HMA04414.1"
                     /translation="MPSPKRIGQIEFGLLSPKEIRKMSVRKIIWADTYDEDGYPYPQG
                     LMDLALGVIDPGLRCKTCDQKASDCPGHFGHIELAKPVIHVGYTRLIRKLLRVTCRAC
                     SRLLLEPVEIEKVLSSDEESPGADILKEKDIKKERACPHCGEQQLKINFEKPTTFSEI
                     WTEDGKRMEHKLTPADVRARLEKIPDEDLRLLGINPDVARPEWTILTVLPVPPVTMRP
                     SIILENGQRSEDDLTHKLVDIIRINQRFKENQDAGAPHLIIEDLWELLQYHVTTYLDN
                     EVAGCPPARHRSGRPLKTLSQRLKGKDGRFRGSLSGKRVNFSARTVISPDPNIKVTEV
                     GVPLAVAKEMTVPVRVTPFNKDEMREFIMRGPVRPDVHAPVGANYVIRPDNRRLRLSD
                     ANLETVVAMVEPGWTVERQIRDGDIVLFNRQPSLHRMSIMAHRVRVMEGKTFRLNPAV
                     CPPYNADFDGDEMNLHVPQTEEAKAEAHILLAVEENILSPRFGGPIIGGIHDHVSGIF
                     MLTHGLRHYTKDQVIYLLKHYALERMPEPEKEEDGRELWSNKQVFSAILPPDLNMVFK
                     ASSCASCETCKREECERDAYVKIFDGKLVSGTIDKKAIGAFDGQILHRVIRQYGMKKA
                     AEFIDEMTKLAIRAIMLDGFSFGIDDEDLSRTEHGQIREVLENASTDVERRIKIFQDG
                     QLEPMPGRTLDETLEMQIMQVLGKARDRTGEIAGRHLGLENSAVVMAVSGARGSMLNL
                     TQMAGCIGQQSVRGERIVRGYDDRTLPHFRKGDRGAAAHGFIQNSYKSGLSPTEFFFH
                     AIGGREGLVDTAVRTSQSGYLQRRMINALQDLKVAYDGTVRTTGGRIIQFRYGEDGTD
                     PSKSSFGSPVDVKGIIESELKEEVK"
     gene            complement(3894..5708)
                     /gene="rpoB"
                     /locus_tag="VKO45_00650"
     CDS             complement(3894..5708)
                     /gene="rpoB"
                     /locus_tag="VKO45_00650"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HMA04415.1"
                     /translation="MKKSRVFVDGALIGLVENPKEHVETLRKMRRKGEISTEINISFK
                     EYNGDVIIHTDRGRARRPLIVLENGKPLITPEDIAKLSDGLVPFDDLVKRGLVEFIDA
                     EEEEDLFIAIHEKDITPEHTHLEIDPSLILGIAAAHVPFPEHNASPRVTMGAGMVKQA
                     LGFGAANMKLRPDTRGHLLHYAERPIVHTSTSESIGSDDRPAGQNFVVAILSYEGYNI
                     EDALIFNKAAIDRGLGRSHFFRTYEGEERRYPGGQVDKIQIPDEEVTGAHGAEFYQHL
                     DADGIINPETVVFEKDVLIGKTSPPRFLEEPSGELIAVEKRRDTSVTMRSNERGIIDT
                     VIITEGENSARLVKVRTRDLRVPEVGDKFASRHGQKGVIGLIAPAEDMPFTESGITPD
                     IVINPHAIPSRMTIGHMLEMLGGKVGSMEGRRINATAFSGESEVDLRASLKKLGFAHN
                     GREVMYDGITGRAFATDIYVGIIYYQKLYHMVSSKMHARSRGPVQVLTRQPTEGRARE
                     GGLRFGEMERDVMIGHGAAMALKERLLDESDKVTQYVCAHCGMIAMLDRKQNTTRCLA
                     CGGETDIYPVEMSYAFKLLLDEMKSMGIAPRLKLQDMV"
     gene            complement(5705..7264)
                     /locus_tag="VKO45_00655"
     CDS             complement(5705..7264)
                     /locus_tag="VKO45_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HMA04416.1"
                     /translation="MIERGVLSRVYFSKEHVSRHQLDSFNNFLEHNLQKVVNEQKIIQ
                     TDIDSRGKSRVPVWVELGTITVEKPVVREADGSQGSLFPSEARLRNLTYAAPIYLTMT
                     LMHGDTRQDPVVANIGQLPVMVGSRKCNLFGQSDQERVAHGEDPLDPGGYFIINGTER
                     VLMTLEDLASNKIMTEFTERYNERIYVAKVFSQFRGYRALVVVERNRKNLLEVSFPSV
                     AGHLRFVDLMKALGLASDHDIVEAVSTDEEILTFMMQNLEESECDSVESGIMYVGKKL
                     APNQTRDYQKKRAEFVLDNYLLPHLNYLMPANLKEGDPGYRAAVDGVRLAKAHFLGRM
                     AESCFDLVLNRRRIDDKDHYANKRLKLAGDLMEDLFRISLNRLTRDIKYQLERASMRH
                     RDLSIATAVRADVLTERLLHPLATGNWVGGRTGVSQLLDRVDHMSVLSHLRRVISPLS
                     RSQPHFEARDLHPTQWGRICPSETPEGPNCGLVKNFAQMVEISRGVDDDSEVPRLLYG
                     LGVEQIRRETA"
     gene            complement(7280..7528)
                     /locus_tag="VKO45_00660"
     CDS             complement(7280..7528)
                     /locus_tag="VKO45_00660"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HMA04417.1"
                     /translation="MTTFNVLEHVMVPDHRIMEEGEIKSLLSTYHLTLEQLPKIYHDD
                     PAVKAVKAKAGDVIRIVRESQTAGRAESYRLVIRRPKK"
     gene            complement(7629..7702)
                     /locus_tag="VKO45_00665"
     tRNA            complement(7629..7702)
                     /locus_tag="VKO45_00665"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7666..7668),aa:Lys,seq:ttt)
     gene            7936..8370
                     /locus_tag="VKO45_00670"
     CDS             7936..8370
                     /locus_tag="VKO45_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HMA04418.1"
                     /translation="MEIDDTIRNMTGIISLITKVIYFITAVILSVLALVSFVVAAYAF
                     LALLGPGDLTTSLLSILHAILLTIIIVEILETITAYFKTSKVLVRPILVAGLTAMVRR
                     VLVLGVETINPTDILVTVAVIAVLTAAVVLVGKEELQEEKPC"
     gene            complement(8394..9029)
                     /locus_tag="VKO45_00675"
     CDS             complement(8394..9029)
                     /locus_tag="VKO45_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMA04419.1"
                     /translation="MYTILVVDDSPFIVDVFVTMLERGGYRAVAAYSGLECLDILKNI
                     KPDLILLDIMMEPIDGWETLEQIKRNPETKEIPVLMLTAKQLSPEEAQTYGMYIEDYV
                     LKPITHRELYDTIEHVLKRRNTIKLDVEEAKKAGFDQGIIDEYSRLAKSVDVNNRLLK
                     ILETTYNIYDSNQQVSSKISQAMKSMSESIKNQEGRLTQIRTELDRVLNEK"
     gene            9482..10237
                     /locus_tag="VKO45_00680"
     CDS             9482..10237
                     /locus_tag="VKO45_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04420.1"
                     /translation="MQLPRGKFRSVIKGVGLNSLIQELAEVRYTGTISISWKREAITL
                     VIQKGKYTLAEYGPLRGDMAWEEILALGETIIDAILSDFSEPEMKLALEFNDAWLLTP
                     QAVLRPLRTPRERVRETAHPRAAPEPVARKAPPAEPARRAPVPAPPEEPEIPPATKKV
                     PARESPVIAEVPESPPAWTKVDMTQAIAMAADQAGEEAPPPGGYDELALLHMEMSSLD
                     DVDVEHIATKIRKNAKGIVKKLHLGHLMTEKEE"
     gene            10249..10833
                     /locus_tag="VKO45_00685"
     CDS             10249..10833
                     /locus_tag="VKO45_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04421.1"
                     /translation="MTAEFESLAGMLLIILSITCIVQVIFVGLLLWYVKRMAESLAGA
                     RAGAVPSPTGAGEGVPATVPPAEEAMVPASPVPVGVAAPPAPGTEILGDSPDIQESIR
                     RLCKKYALSDFIISTLDGLVVVSLSPGSSEDAARFSDLYRRKRRPDSPGVFFLEVSHR
                     GEAMLAIARSDLAPGPDQIRDIGEDARKILEWWL"
     gene            10890..11675
                     /gene="minD"
                     /locus_tag="VKO45_00690"
     CDS             10890..11675
                     /gene="minD"
                     /locus_tag="VKO45_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATPase MinD"
                     /protein_id="HMA04422.1"
                     /translation="MIRAYTIASGKGGTGKTTCTINLGTSLAKLGLETFIMDADIGMA
                     NIGLLLGLEDAPITIHEVLAGKAKLEEAIYDGPSGVKVLPSGISLQGFQLANPDRFRD
                     IMKELVNRCDYLLIDAPAGISRDGVIPLAIADEVILVVNPEVSSIVDALKTKVLTEVV
                     GGHVACAILNRAGSEHTELIQSKLEKTLGVKLIGIIPEDPNVRVAAVYKTPVVMKFPG
                     SEASRAFRKIASTIAGIAPEESVEAATDGFVSRFVKILFKGAK"
     gene            11672..11878
                     /locus_tag="VKO45_00695"
     CDS             11672..11878
                     /locus_tag="VKO45_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04423.1"
                     /translation="MTTEELYAVIIFILGVLILFQVFFMYLMRVRMRQLQFEMEQGSS
                     RVDLSDIELEALTRNVEAFKRLKI"
     gene            12000..12073
                     /locus_tag="VKO45_00700"
     tRNA            12000..12073
                     /locus_tag="VKO45_00700"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12035..12037,aa:Pro,seq:ggg)
     gene            12104..12952
                     /locus_tag="VKO45_00705"
     CDS             12104..12952
                     /locus_tag="VKO45_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HMA04424.1"
                     /translation="MKIHDRCYDCLLSRISYECRLSTTDEGRIAHVLSECQALLGGFR
                     GIPRPAPLVASAAHRRAYELLGDPDPYRRIKEESTREAARGCRAVRGFLTSFRDHALA
                     CAIANTFDYGVSSHRVTADFEAFFREEFRKGFLIDETGRMEEIAGEVVYFTDNAGEIL
                     FDRLFVGYLAGRGSRVTVAVKGAPILNDATIREAEQAGLPRVARVTTTGSGDIGVDLG
                     KIPPDLAEALGRSTLVIAKGMANYEALTEYRDLPPVGYLMAVKCPTIAESVGAPEGSL
                     VALLEE"
BASE COUNT         2215 a         4212 c         4227 g         2609 t
ORIGIN      
        1 ctgttctgtc ccaggaagac tttcccggtt tttaatgctc ttctcagtga gatgttgaaa
       61 tccatgtgcg attctcctag tgcatgggtt ttgcgatgag tttcacgtcg ccggtgccca
      121 gctggatggg ctgtccgacg atgatgttct cagtgacacc gttcagttcg tccacctcgt
      181 tcgccacggc cgcgtcgagg aggtggttca ccgtcacctc gaaggcggcc cttgagagga
      241 ccgactcctt ctccccggcg atgccgtgtc tcccgatctg cttcacctcg ccgtccatgc
      301 acatcatgtc cgcgacgagc atgatgtgcc ggacgtcgac gaggataccc tgctcggcga
      361 gggtggagag ggcctcgtag atgatggagt tccgggccgc ctcgatgccg aggacgtctg
      421 cgatctcgct gatgttgttc gtccgggtcc gggtggtatc cacccccgcg acctcgaaca
      481 catccgaaag attggagccc tcggtatata ggatgtactc cccctcctcc ttccggacga
      541 ccacccgctc gatgtcatcg atcccctgga cgatgacgtt tcgcacgtgc tcggcgagct
      601 ggaacaggtt ctggtagctc tcccggtcct tggggaggaa gaggagccgg gccgcctcct
      661 cctcgacctc gcactcgaag tcccggtagt ggcgccgctc cttgatcttc ttcggggcga
      721 gctccatgat ccggtccacc ccgatcttcc gcttgtcggt gaccgcggtg ttcagctgga
      781 cgaggacgag catcttctcc atgtcgatgg tgatgtcccc gaactcggcg aggggcgcgg
      841 cctcgacctg ccagctcacc tcccgggccc tgtcccggtc ggaggcgtac tccggcttca
      901 ggtggatggt catggtgggc gtggagggct cccgccgggc gtccatgatc tcgatgagcc
      961 gggggaggcc cagggtgacg ttgatctcgg cgacccccgc gtagtggaag gtccgcatgg
     1021 tcatctgcgt ccccggctcg ccgatggact gggcggccac gatgcccacg gcctcgcagg
     1081 cctcgatccg ggtgttctgg tactccctca cgatccggtc gaggatcttc gtgaactgct
     1141 tgtcggtgaa ggaccggtcg gcgaggaact tcttcatctc ctcccgggtc cggtccggga
     1201 gctccgccgc gtcgatccgc ttctccatgg actccttcat ttaacctcct ccttgagctc
     1261 gctctcgatg atccccttca cgtccacggg gctcccgaaa gaggacttgg acgggtcggt
     1321 cccgtcctcc ccgtaccgga actggatgat ccgcccgccg gtggttctca ccgtcccgtc
     1381 gtaggccacc ttcaggtcct ggagggcgtt gatcatccgc cgctgcaggt acccgctctg
     1441 cgatgtccgg acggcggtgt ccacgagccc ctcgcgcccg cctatggcat ggaagaagaa
     1501 ctcggtcggg gagaggccgc tcttgtagct gttctggatg aagccgtggg ccgccgcgcc
     1561 ccggtcgccc ttccggaagt gggggagggt gcgatcgtcg tagccccgga cgatccgttc
     1621 cccacggacc gactgctgac cgatgcagcc ggccatctgg gtgaggttga gcatcgaccc
     1681 ccgggcgccc gagacggcca tgaccacggc gctgttctcg agcccgaggt gccggcctgc
     1741 gatctcgccg gtccggtctc tcgccttccc gaggacctgc atgatctgca tctccagggt
     1801 ctcgtccagg gtccggccgg gcatgggctc gagctggccg tcctggaaga tcttgatccg
     1861 ccgctccacg tccgtggatg cgttctcgag gacctcccgg atctgaccgt gctcggtgcg
     1921 ggagaggtcc tcgtcgtcga tcccgaagga gaagccgtcg agcatgatgg cccggatggc
     1981 gagcttggtc atctcgtcga tgaactcggc ggccttcttc atcccgtact ggcggatgac
     2041 ccggtggagg atctggccgt cgaaggcccc gatggccttc ttgtcgatgg tgcccgagac
     2101 gagcttgccg tcgaagatct tcacgtaggc gtcccgctcg cactcctcgc gcttgcaggt
     2161 ctcgcagctc gcgcaggagg aggccttgaa gaccatgttc aggtcgggcg ggaggatggc
     2221 cgagaagacc tgcttgttgg accagagctc cctcccgtcc tcctccttct ccggctcggg
     2281 catccgctcg agggcgtaat gcttcaggag gtagatgacc tggtccttcg tgtagtgccg
     2341 gagcccgtgg gtgagcatga agatccccga gacatggtcg tggatcccgc cgatgatggg
     2401 ccccccgaac cggggcgaga ggatattctc ctccacggcg agaaggatgt gggcctcggc
     2461 cttggcctcc tcggtctggg gcacgtggag gttcatctcg tccccgtcaa agtcggcgtt
     2521 gtagggcggg cagacggcgg ggttgagccg gaaggtcttc ccctccatca ccctcacccg
     2581 gtgagccatg atggacatcc ggtggaggga gggctgccgg ttgaagagga cgatatcccc
     2641 gtcccggatc tgccgctcca cggtccaccc tggttcgacc atcgcgacca ccgtctccag
     2701 gttcgcatca gacagccgga gccggcgatt gtccggccgg atgacatagt tcgcccctac
     2761 gggtgcatgg acgtccgggc ggacgggccc ccgcatgatg aactccctca tctcgtcctt
     2821 gttgaagggg gtcaccctga ccggtacggt catctccttg gcaacggcga ggggcacccc
     2881 gacctcggtg accttgatgt tcgggtcggg cgagatcacg gtacgggccg aaaagttcac
     2941 ccgcttcccc gagagggaac cccggaaacg gccgtccttc cccttgagcc gctgggagag
     3001 ggtctttaag ggcctcccgc tccggtgacg ggcaggagga cagcctgcaa cctcgttgtc
     3061 caggtacgtg gtgacatggt actggaggag ctcccagaga tcctcgatga tgaggtgcgg
     3121 ggcaccggcg tcctggttct ccttgaagcg ctggttgatc cggatgatat ccacgagctt
     3181 gtgggtgagg tcatcctcgg accgctggcc gttctcgaga atgatggagg ggcgcatggt
     3241 gaccggcgga accgggagca ccgtgaggat ggtccactcc ggcctcgcga cgtcggggtt
     3301 gatcccgagg agccggaggt cctcgtccgg gatcttctcg agccgtgccc ggacgtcggc
     3361 gggggtgagc ttgtgctcca tccttttgcc gtcctcggtc cagatctcgg agaaggtcgt
     3421 gggtttctcg aagttgatct tgagctgctg ctcgccgcag tgggggcagg cccgctcctt
     3481 cttgatgtcc ttctccttga ggatgtccgc ccccggggac tcctcgtcgg aggaaaggac
     3541 cttctcgatc tcgaccggct cgaggaggag acggctgcag gcccggcagg tgacccggag
     3601 gagcttccgg atgagcctcg tgtaccccac gtggatgacc ggctttgcga gctcgatgtg
     3661 gccgaagtgg cccgggcagt ccgaggcctt ctggtcgcag gtcttgcacc ggaggccggg
     3721 gtcgatgacc ccgagggcga ggtccatgag cccctggggg tacgggtacc cgtcctcgtc
     3781 gtaggtgtcc gcccagatga tcttgcggac actcatcttc cggatctcct ttggcgagag
     3841 aagaccgaac tcgatctgtc ctatgcgttt tggactcggc atcgtcgcac ctcctacacc
     3901 atgtcctgca gtttcagccg tggcgcgatc cccatggact tcatctcgtc caggaggagc
     3961 ttgaaggcat aactcatctc aacggggtag atgtcggtct ccccgccgca ggcgaggcac
     4021 cgggtggtgt tctgcttgcg gtcgagcatc gcgatcatcc cgcagtgggc gcagacgtac
     4081 tgggtgacct tgtctgactc gtcgaggaga cgctccttga gggccatcgc cgccccgtgg
     4141 ccgatcatca cgtcgcgctc catctccccg aaccggaggc cgccctccct cgcacgcccc
     4201 tcggtgggct ggcgggtgag gacctggacc gggccccggg accgggcgtg catcttcgag
     4261 gagaccatgt ggtagagctt ctggtagtag atgatcccga cgtagatatc ggtggcaaag
     4321 gccctcccgg tgatcccgtc gtacatcacc tcgcggccgt tgtgggcgaa cccgagcttc
     4381 ttcagggatg cccggagatc gacctcggac tcgccggaga acgcggtggc gttgatccgc
     4441 cgcccctcca tggaccccac cttcccgccg agcatctcga gcatgtggcc gatggtcatc
     4501 cggctcggga tggcgtgggg gttgatgacg atgtccgggg tgatcccgct ctcggtgaag
     4561 ggcatgtcct ccgccggggc gatgaggccg atgaccccct tctgcccgtg ccgggaggcg
     4621 aacttgtccc cgacctcggg cacccggagg tcccgggtcc gcaccttcac gagccgggcg
     4681 ctgttctccc cctcggtgat gatgaccgtg tcgatgatcc cccgctcgtt ggaccgcatg
     4741 gtgaccgagg tgtcccgccg cttctccacg gcgatgagtt caccggaggg ctcctccagg
     4801 aacctcgggg gagaggtctt cccgatgagg acgtccttct cgaagaccac cgtctcgggg
     4861 ttgatgatcc cgtcggcgtc caggtgctgg tagaactccg ccccgtgggc gcccgtcacc
     4921 tcctcgtccg ggatctggat cttgtccacc tggccgccgg ggtaccggcg ctcctcgccc
     4981 tcgtaggtcc ggaagaagtg ggaccggccg aggccccggt cgatggcggc cttgttgaag
     5041 atgagggcgt cctcgatgtt gtacccctcg tacgagagga tggcaacgac gaagttctgt
     5101 cccgcaggcc ggtcgtcgga accgatggac tccgaggtgc tcgtgtggac gatggggcgc
     5161 tccgcgtagt ggaggaggtg cccccgggta tcaggccgga gcttcatgtt cgccgcgccg
     5221 aagccgaggg cctgcttcac catccccgcc cccatggtga cccgggggct cgcgttgtgc
     5281 tcggggaagg ggacatgggc ggccgcgatg cccaggatga gcgacgggtc gatctcgaga
     5341 tgggtgtgct cgggggtgat atccttctcg tggatggcga tgaagaggtc ctcctcctcc
     5401 tcggcatcga tgaactccac gaggccccgc ttcacgaggt cgtcgaaggg gacgagcccg
     5461 tccgagagct tcgcgatgtc ctccggggtg atgaggggct tcccgttctc gaggacgatg
     5521 aggggcctcc tcgcccgtcc ccggtcggtg tggatgatga cgtccccgtt gtactccttg
     5581 aaggagatgt tgatctcggt ggagatctcg cccttccggc gcatcttccg gagggtctcg
     5641 acatgctcct tcgggttctc gacaaggccg atgagggccc cgtccacaaa gacccgggac
     5701 ttcttcatgc cgtctccctc cggatctgct cgacaccgag gccgtagagg agcctcggga
     5761 cctcggagtc gtcgtccaca ccccggctga tctcgaccat ctgggcgaag ttcttcacga
     5821 ggccgcagtt cggcccctcg ggggtctcgg acggacagat ccggccccac tgggtcgggt
     5881 gcaggtcccg ggcctcgaag tggggctggg accgggagag cggggagatg acccttctca
     5941 ggtgcgagag gaccgacatg tggtccaccc ggtccagaag ctgggaaacc cccgtcctcc
     6001 cgccgaccca gttgcccgtg gcaaggggat ggaggagccg ctcggtgagg acatcggccc
     6061 ggaccgccgt cgcgatggag aggtcccggt gccgcatgga ggcccgctcg agctggtact
     6121 tgatatctct cgtgagccgg ttcagggaga tccggaagag gtcctccatc aggtcacccg
     6181 cgagcttgag acgcttgttc gcgtagtggt ccttgtcatc gatccgccgg cggttgagca
     6241 cgagatcgaa gcaggactcg gccatccggc cgaggaagtg cgcctttgcc agccggacac
     6301 cgtctaccgc ggcccggtac ccggggtctc cttccttcag gttcgcgggc atgaggtagt
     6361 tcaggtgggg gaggaggtag ttgtcgagga cgaactcggc ccgcttcttc tggtagtccc
     6421 gggtctggtt cggggcgagc ttcttgccca cgtacatgat cccgctctcc accgagtcgc
     6481 actcgctctc ctcgaggttc tgcatcatga aggtgaggat ctcctcgtcg gtcgaaacgg
     6541 cctccacgat gtcgtggtcc gaggcgaggc cgagggcctt catgaggtcc acgaacctga
     6601 ggtggccggc gaccgagggg aaggacacct cgaggaggtt cttccggttc cgctcgacca
     6661 cgacgagggc ccggtacccc cggaactggg agaagacctt ggccacgtag atcctctcgt
     6721 tgtaccgctc ggtgaactcg gtcatgatct tgttcgaggc gaggtcctcg agggtcatga
     6781 ggacccgctc ggtcccgttg atgatgaagt agccgcccgg gtcgagggga tcctcgccgt
     6841 gggcgaccct ttcctggtcg gattgcccga agaggttgca cttccgggac cccaccatga
     6901 ccgggagctg cccgatgttc gcgaccaccg ggtcctgcct cgtgtccccg tgcatgaggg
     6961 tcatggtgag gtagatgggg gcggcatagg tgaggttccg cagcctcgcc tcgctgggaa
     7021 agaggctccc ctgcgacccg tcggcctccc gcaccaccgg tttttcaacg gtaatggtcc
     7081 cgagttccac ccagaccggc acccggctct tcccccggga atcgatgtcg gtctggatga
     7141 tcttctgttc atttaccacc ttctggagat tatgctcgag aaaattgttg aaggagtcga
     7201 gctggtggcg cgatacatgc tccttcgaga aatagactct ggagagaaca cccctttcga
     7261 tcagaagtac caggccccct tacttcttcg gccttcgtat gacgaggcgg taggactcgg
     7321 cccttcccgc cgtctggctc tcgcgaacga tccggatgac gtcccctgcc ttggccttga
     7381 cggccttcac ggccgggtcg tcgtggtaga tcttcggcag ctgttccagc gtgagatgat
     7441 acgtcgagag gagggacttg atctccccct cctccatgat gcgatggtcc gggaccatca
     7501 catgctcaag cacattgaat gtggtcaagc aaccctccca ggaaagttga atttccccgt
     7561 ccccgaaaca agatagaggt taatcagatc catcaccgct ctcatccaca gatgtctcct
     7621 ctgtggagcg ggctcggggg gatttgaacc cccggccacc tggttaaaag ccaggcgctc
     7681 tacctagctg agctacgagc ccccgcaccc tcgccccttt tggcattatt acaacgcgag
     7741 gaaactatat aaacgttctt ccccgagggt gtatccggcc ccccgtccgc gggatgccac
     7801 cggtttgggt gcgggccggg aggggcagag accgcgatgg tccgcgggga atcgatactt
     7861 attttataga atcggatcgc aatatccaga aggatattca ggggatcgat ccggcctgcc
     7921 cggaccggga gcaagatgga aatcgacgac accattcgga acatgacggg aatcatctcc
     7981 ctgatcacca aggtcatcta ctttatcacc gcggtgatcc tctcggtcct tgccctggtc
     8041 tccttcgtgg tggcggccta cgccttcctc gccctcctcg gaccggggga cctcaccacg
     8101 agcctcctct ccatcctcca cgccatcctc ctcaccatca tcatcgtcga gatcctggag
     8161 accattaccg cctacttcaa gacgagcaag gttctcgtcc gccccatcct cgtcgccggc
     8221 ctcacggcca tggtccggcg cgtcctcgtc ctgggcgtcg agaccatcaa ccccaccgac
     8281 atcctggtaa cggtcgcggt catcgcggtc ctcacggccg cggtggtcct cgtggggaag
     8341 gaggagctcc aggaagaaaa gccctgctga aggtccggga acggaaaggg aatttatttc
     8401 tcgttgagga cccggtccag ctcggtccgg atctgggtga gccggccctc ctggttcttg
     8461 atgctctcgg acatgctctt catggcctgg gagatcttgg aggagacctg ctggttcgaa
     8521 tcgtagatat tatacgtcgt ctcgaggatc ttgaggagcc ggttgttcac gtccacgctc
     8581 tttgcgaggc gcgagtattc gtcgatgatc ccctggtcga agcccgcctt cttcgcctcc
     8641 tcgacatcga gcttgatggt gttcctcctc ttgaggacat gctcgatggt gtcgtagagc
     8701 tcccggtggg tgatgggctt gaggacatag tcctcgatgt acatcccgta ggtctgggct
     8761 tcctcgggag agagctgctt tgcggtgagc atgagcaccg ggatctcctt cgtctccggg
     8821 ttcctcttga tctgctcgag ggtctcccat ccgtcgatgg gttccatcat gatgtcgagg
     8881 aggatgagat cgggcttgat gttcttcagg atgtccaggc actcgagacc gctgtacgcg
     8941 gcgacggccc ggtagcctcc ccgctcgagc atggtcacaa agacgtcgac gatgaagggg
     9001 ctgtcgtcga cgacgaggat ggtgtacatg tcaggcctcc ccttcccgga tccccgatgg
     9061 cggggcctcg cgagggctcg taaaaacagt cattcccaca ttttccggag tcatgtgcat
     9121 tccgttgata cccaccggga ttccacccgg acaccctccg gttgccggat ggggccgttt
     9181 cgttccctta atagatgatc actgttccgt tcggtaataa acttatttgt ttcccccctt
     9241 ccctgccata atttccctat tgaccacccg tacacctcat cttcattttc cgaaatgatt
     9301 aagatacccg gaacgcacta gcccggtgcc ccggcggtgg ggctgcgccg gcccggtggc
     9361 ggggaaaagg ggagatatct ccagaactcc ccctgatctg gctccaaagt taaaaaaagg
     9421 gattgacaac gaggggcagg aacaagaaac attatttaag ttcacacacc agctatttac
     9481 catgcagctt ccccggggga aattccgttc cgttatcaag ggagtggggc tgaattccct
     9541 catccaggag ctcgctgagg tccggtacac cggaaccatc agcatctcct ggaaaaggga
     9601 ggccatcacc ctggtcatcc agaaggggaa gtacaccctc gccgagtacg gcccgctcag
     9661 gggggacatg gcctgggagg agatcctcgc cctcggggag accatcatcg atgccatcct
     9721 ctcggacttc tccgagcccg agatgaagct cgccctcgag ttcaacgatg catggctcct
     9781 caccccccag gccgttctcc ggccgctgag gaccccccgg gagagggtga gggaaacggc
     9841 ccatccccgg gcggccccgg agccagtggc gaggaaggcc cccccggcgg aacccgcccg
     9901 gcgggcgccg gtccccgccc ctcccgagga gcccgaaatt cccccggcca ccaagaaggt
     9961 tcccgccagg gaatcccccg tcatcgcgga ggttcccgag tccccgccgg cctggacgaa
    10021 ggtggacatg acgcaggcca tcgccatggc ggcggaccag gccggggagg aggcgcctcc
    10081 tccgggggga tacgacgagc tcgccctcct ccacatggag atgtcctccc tggatgacgt
    10141 ggacgtggag catatcgcga cgaaaatccg gaagaacgcg aaggggatcg tgaagaaact
    10201 gcacctcggc catctcatga ccgagaagga ggaatgaggg gacctgtcat gacggccgag
    10261 ttcgagagcc tggcggggat gcttctcatc atcctcagca tcacctgcat cgtccaggtt
    10321 atcttcgtgg gactgctcct ctggtacgtg aagaggatgg cggaaagcct cgccggggcc
    10381 cgtgcagggg cggtaccctc ccccaccggt gcgggagagg gtgttccggc caccgtcccg
    10441 ccggcggaag aggcgatggt acccgcctcc ccggtaccgg tgggcgtggc ggcgccaccg
    10501 gcccccggca cggagatcct tggggacagt ccggatatcc aggagagcat ccggcgtctc
    10561 tgcaagaagt atgccctctc cgatttcatc atctccacgc tggacggcct cgtcgtggtg
    10621 tccctctccc cgggatcctc ggaggacgcg gcccggttct cggacctcta ccggcggaag
    10681 aggaggcccg attcgccggg ggtgtttttc ctcgaagtct cccaccgggg ggaggcgatg
    10741 ctcgccatcg cgaggagcga cctggcgccc ggcccggatc agatccggga tatcggggag
    10801 gatgcccgga aaattctcga atggtggctc tgatggggac tgggaaacgt tatatgaatt
    10861 tccggccaat gcaactgtac agaggctgaa tgatccgggc ttacaccatc gcatccggta
    10921 aggggggaac cggaaagacc acctgcacca tcaacctggg gacctccctc gccaagctgg
    10981 gcctcgagac cttcatcatg gatgcggaca tcgggatggc gaacatcggc ctcctcctcg
    11041 gcctcgagga tgcccccatc accatccacg aggtcctcgc cgggaaggcg aagctcgagg
    11101 aggccatcta cgacggtcca agcggcgtga aggtcctccc gagcggcatc tccctccagg
    11161 gtttccagct cgcaaacccc gaccggttcc gggacatcat gaaggagctc gtgaaccggt
    11221 gcgactacct cctcatcgac gccccggccg ggatcagccg ggacggggtg atccccctcg
    11281 ccatcgccga cgaggtcatc ctcgtggtga accccgaggt ctcctccatc gtggatgccc
    11341 tgaagaccaa ggtgctcacc gaggtggtcg gggggcatgt cgcctgcgcc atcctgaacc
    11401 gggccggctc cgagcacacc gagctcatcc agagcaagct cgagaagacc ctcggcgtga
    11461 aactcatcgg gatcatcccc gaggacccga acgtgcgggt cgcggcggta tacaaaaccc
    11521 cggtcgtcat gaaattcccg gggagcgagg cgtctcgcgc cttccggaag atagcctcca
    11581 ccatcgccgg gatcgcgccg gaggagagcg tggaggcagc caccgacggg ttcgtcagcc
    11641 ggttcgtgaa aattcttttc aagggggcaa aatgaccacc gaagagctgt acgccgtgat
    11701 catcttcatc ctcggcgtcc tcatcctgtt ccaggtcttc ttcatgtacc tgatgagggt
    11761 ccggatgcgc cagctccagt tcgagatgga gcagggctcc tccagagtcg acctctcgga
    11821 catcgagctc gaggccctca cccggaacgt cgaggccttc aagcggctga agatctgatc
    11881 cacgggaacc gggtcgggag gaggttttca ctgatgggtg aaggccttca agcggctgaa
    11941 gatctgatta tcctcgacaa agagaaggta cataagggat cgggtcccat gaaagtggag
    12001 gggccatagg gtagcctggt tatcctagga gactgggggt cttctgacct gcgttcaaat
    12061 cgcagtggcc ccatctcatt ccatcttttc ccacggtgtt ccgatgaaga tccatgaccg
    12121 gtgctacgac tgcctcctct cgcggatctc ctacgagtgc cggctctcca ccaccgacga
    12181 ggggaggatc gcccacgttc tctccgagtg ccaagccctc ctcgggggat tccgcgggat
    12241 cccccggccc gcacctctcg tggcctctgc tgcccaccgg cgtgcgtacg agctcctcgg
    12301 cgacccggat ccgtaccgcc ggatcaagga ggaaagcacc agagaggcag cccgtggatg
    12361 ccgggccgtg agggggttcc ttacctcatt ccgggaccat gccctcgcat gtgccatcgc
    12421 gaataccttc gactacggtg tctcctcgca ccgggtcacc gccgatttcg aggcgttttt
    12481 ccgggaggag ttcaggaagg ggttccttat cgacgagacg gggaggatgg aggagatcgc
    12541 gggggaggtc gtgtacttca ccgacaacgc cggggagatc ctcttcgacc ggctcttcgt
    12601 cgggtacctc gcggggaggg gctcccgggt cacggtggcc gtgaaggggg cacccatcct
    12661 gaacgacgcg accatcaggg aggcggagca ggcgggactc ccccgggtgg cccgggtgac
    12721 gaccaccggg agcggggaca tcggcgtcga cctcgggaag atcccccccg atcttgcgga
    12781 ggccctgggc cgctctaccc tcgtcatcgc gaaggggatg gccaactacg aggccctcac
    12841 cgagtaccgg gaccttcccc cggtcggcta cctgatggcg gtgaagtgcc cgaccatcgc
    12901 cgagagcgtc ggtgcccctg aagggtccct cgtggctctt ctggaggagt gacggggctg
    12961 tcgagtccgg ttcaagaatg tagacgctcc tgggggaaga gaggtttcgt aagaaaccga
    13021 cctcgagcga cgaagtcgtg agctcgcttc gctcgccccc gccgctgggg cgtccccctc
    13081 atgaggatag gcgtgtctgg gagccccgat tcacttaccc ggcataacgg ccccgggctc
    13141 acgccggaaa gcagaatttt acgttttctt cgcgcagacg tcttcgaggg ccttcacacg
    13201 ggggttaccg ggatcgatgg ccctcgcctt ccggaagaag gccgctgcat cggcctttct
    13261 ccc
//
LOCUS       DATGDI010000017         7942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_1766, whole genome shotgun sequence.
ACCESSION   DATGDI010000017 DATGDI010000000
VERSION     DATGDI010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1580)
                     /locus_tag="VKO45_00710"
     CDS             complement(<1..1580)
                     /locus_tag="VKO45_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HMA04425.1"
                     /translation="MARWLCQVCGYIYDEEAGEKMTNTPPGTRFEDLPADWKCPICGS
                     GKEVFVKTGEGAPAGQATTVSDMIMGNLAAWGVSLVFGIPGTSSLGLVDAVRRRTDMR
                     YIAVRHEENAAFAASAYNKLTGGIAACLTIAGPGATNLATGLYDAKEDSASVISLNGQ
                     VEMQYTGPGGFQEIDQDAFFRPVTIFNNTIADPKMAVAILEKALRSAVLGRGVSQVSV
                     PNDVQKEPLAAGFCKRESCIPDQDIVPEAGEMRRAADLVARGGNPVILAGWGAFPYGD
                     LVVALAERIRAPLITTFRAKGIVPDDNPWFVSVLGTVGMPEARVLAQEADPLICLGVG
                     FSKMTNVPLDRPMIQLDIDPQKLGKGPSSVSLWGNCHSVLPKWIEMVAPREDPAAGAR
                     IAALKEAIRKRLDAEADAKAIPVRPPFIMKVLSDTVPDDAVISIDIGENGWWFGRNFR
                     MKRQRFVMSGYLASMGFGLPGAIAAKLAHPEKKVFCITGDGGFAMAMAELVTAVKYHL
                     PMVVVVLNNRELGMIRVE"
     gene            1764..2963
                     /locus_tag="VKO45_00715"
     CDS             1764..2963
                     /locus_tag="VKO45_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HMA04426.1"
                     /translation="MESIYRESLELHRRMRGKLEVRGKVPVRTRRDLSRAYTPGVAQV
                     CREIQKNRNRAWIYTIKANTVAIVSDGSAVLGLGNIGGYAAIPVMEGKALLFKDFAGI
                     DAFPICFEGYHTDFIEDVKNIAPVFGGINLEDIAAPKCFEVEEALQDIGIPVMHDDQH
                     GTAVVILAAFLNACRVTGKSLSDITIAMSGAGAAGFATFRLLKCLGYDRKACLPVGDM
                     IICDRMGIIHRDRPDLLKNKYKYIIAHETNREGRTGTLADAMKGADVFIGVSAPRIVT
                     GKMIRSMNPGAVVFAMANPVPEVMPDVARKAGAVVVGTGRSDFPNQINNVLAFPGIFR
                     GALDARASRITDDMKLAAAHALAGMVREPTRARVLPGPLNRKVAWQVAAAVEKAAKEC
                     GCAVEIG"
     gene            2975..4246
                     /locus_tag="VKO45_00720"
     CDS             2975..4246
                     /locus_tag="VKO45_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HMA04427.1"
                     /translation="MLRVYNTMARRRELFLPRFPPVVKFFSCGPSIYQRPHLGNYRTF
                     LYEDVLERYLEYLGYRVERVLPFTDVEDKAIAQAEKEGVTLSELTDRVAGEFLAEARL
                     LRVKPPTWNPRSSTTVEHTVEIIERLLRKGVAYRFGPDIFFDPLKFPRFGKLFRLDMT
                     RWPRTRRRFRKDTYPGVQWNYGDFILWHGARRGETAVWDTRIGRGRPSWNVQDPAMAI
                     PLLGPSLDIHAGGIDNLWRHHDYNLAVAESFTGREYARYWMHGEHLLIGGKKMSKSRG
                     NIVYLSDLTSDGCTPEEVRFFLIYGHYRDRMNMTRENLEMAQEVLRELRTLAGQVAAG
                     AGNPTETGPAGKPASPDLEERFRERMDDDLDVKGAVDGIAGSLQELAARRRRGTLAPE
                     EAAAALTTLHRIDQVFQVIFPLDQTASSGNR"
     gene            4562..5017
                     /locus_tag="VKO45_00725"
     CDS             4562..5017
                     /locus_tag="VKO45_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04428.1"
                     /translation="MAPRINARHITCPGCQEDVFMEELVGGRCPLCGCELDGSEEPCE
                     GFEELLDRSDLTWLVYQYFLFRKFEAMGASPLQTLHLISRFDDPGANGPSYPEGTPFS
                     VEIPLSMLERILPKRCGRCGRLFFAGGRKLVTGDLGRTAENTSYICPSC"
     gene            complement(5020..6459)
                     /gene="proS"
                     /locus_tag="VKO45_00730"
     CDS             complement(5020..6459)
                     /gene="proS"
                     /locus_tag="VKO45_00730"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HMA04429.1"
                     /translation="MAVEEESGPLPRKGDFSGWYHEILWRAGIIDNRYPVKGLYVWYP
                     YGFAFRKHVYGVLRELMDRDHQEALFPLLIPEQEFMKEAEHIKGFENEVYWVTRGGQS
                     PLEVPLALRPTSETAIYPMYALWVRSHADLPLRLYQVVNTFRYETKATRPLIRAREIS
                     SFKEAHTVHATWEDAEEQAKEAIRLYRAFYDRLSIPVLVTRRPEWDKFPGADYTIAVD
                     TIMPDGRTLQIGTVHHLGDNFSRTFGIRYEDEQGDQRYAFQTCYGISERSIAAAIGVH
                     GDDRGLVLPPEVAPVQAIIIPIIVGKRKDEVLGAARSLASDLAAAGLRVQTDERDLRP
                     GAKYYHWEMRGVPLRIEIGPRDIDRRVVTLVTRLGEKSEAARETVASSVAGVLSGLAG
                     ALLARAVSEMRNRISAPLTLEEAPAAVGKGVAVVSWCRARECAEVLEGKTGGSILGTD
                     IGGNQLPEISKNCIVCGKEGTPTLLARTF"
     gene            complement(6470..7042)
                     /locus_tag="VKO45_00735"
     CDS             complement(6470..7042)
                     /locus_tag="VKO45_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HMA04430.1"
                     /translation="MSDSDLPLRTAAVVALAAFLLTAVAGVALVRQSPSTGQQFIQVI
                     RDQVMGKVDAREPVALAIFIFANNLQACVLLFIGGATLGILTLAIIASNGVIIGGIME
                     IVREERGLLYVAAAVLPHGIFEIPSLILSASLGFIVGSALLAEWRGGGDSAATAAAHG
                     RTFLLVIVPLVAIAAFIEAFITPQIIHLVT"
     gene            complement(7081..7926)
                     /locus_tag="VKO45_00740"
     CDS             complement(7081..7926)
                     /locus_tag="VKO45_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HMA04431.1"
                     /translation="MIGDFINKYYIAGIVQGQPYNIVDTATYAIVLVISVYLVYRWLR
                     RSGVALDGPFILATIPFVVLGGLLRVVEDTGMIGPPAKYLLITPLIFFVVFFIAAAAL
                     VLSRTLEKHRLVPRFRTFYGGIGVGASVAVALLLAWFGLTRTTIDFVVLAAILAIAAA
                     TSLAVMALLQLVFRWEYASDPLFRLLVIGQMLDASATGYGIDFGPIPYQEVHVVGSAL
                     IRWAGTAFALFPLKLVVVIPAIYVLELYRREGNREFANLVLLAMIVVGLAPGVRDMVR
                     MVLHV"
BASE COUNT         1433 a         2596 c         2638 g         1275 t
ORIGIN      
        1 tgttcgacgc ggatcatccc gagctcccgg ttgttcagga cgacgaccac catagggagg
       61 tgatacttca cggcggtcac gagctcggcc atggccatgg cgaaaccgcc gtcgccggtg
      121 atacagaaga ccttcttctc cgggtgggcg agcttcgccg cgatggcgcc ggggaggccg
      181 aagcccatgg aggcgaggta gccggacatc acgaagcgct gccgcttcat ccggaagttc
      241 ctgccgaacc accacccgtt ctccccgatg tcgatggaga tgacggcatc gtcggggacg
      301 gtgtccgaga gcaccttcat gataaagggg gggcggaccg ggatggcctt cgcatcggcc
      361 tcggcatcga gccgtttccg gatcgcctcc ttcagggccg cgatcctcgc acccgccgcc
      421 gggtcctccc tcggcgcgac catctcgatc catttcggga ggacggagtg gcagttgccc
      481 cagagactta ccgacgaggg gcccttgccg agcttctggg ggtcgatgtc cagctggatc
      541 atgggccggt ccagggggac gttggtcatc ttcgagaacc cgacccccag gcagatgagg
      601 gggtcggcct cctgggcgag gacccgggcc tcgggcatcc cgacggtccc gaggaccgag
      661 acgaaccagg gattgtcgtc gggaacgatc ccctttgccc ggaaggtggt gatgaggggg
      721 gccctgatcc tttcggcgag ggcaaccacg agatcgccgt aggggaaggc cccccacccg
      781 gcgaggatga cggggtttcc cccccgggcc acgaggtctg cggcccgtcg catctccccc
      841 gcttcgggga cgatgtcctg gtccgggatg cagctctccc gcttgcagaa gcccgcagcg
      901 aggggctcct tctgcacgtc gttcgggacc gagacctggg agacaccccg gccgagcacc
      961 gcggaccgga gagccttctc gaggatggcc acggccatct tgggatcggc gatggtgttg
     1021 ttgaagatgg tgaccggccg gaagaaggcg tcctggtcga tctcctggaa cccgcccggt
     1081 ccggtgtact gcatctccac ctgcccgttc agggagataa ccgaggcgct gtcctccttt
     1141 gcgtcgtaga ggccggtggc aaggttcgtc gcccccgggc ccgcgatggt gagacaggcg
     1201 gcgatcccac cggtgagctt gttgtacgcc gaggcggcaa aggcggcatt ctcctcgtgc
     1261 ctgaccgcga tgtaccgcat gtcggtcctc ctgcgcaccg catccacgag gccgagggag
     1321 gaggtgcccg ggatcccgaa cacgagggaa accccccatg ccgccaggtt ccccatgatc
     1381 atgtctgaga ccgtggtggc ctggccggcg ggcgccccct ccccggtctt cacaaagacc
     1441 tccttccccg agccgcagat cgggcacttc cagtcggccg gaaggtcctc gaaccgggtc
     1501 cccggcgggg tattggtcat cttctctccc gcctcttcgt cgtagatgta accgcacacc
     1561 tggcagagcc acctggccat cgtcgtacct cgcagatttt acgatccgcc caccggtata
     1621 taaggacacc gttccttcgc aggagttgag gggcaaggta ttatggagcg gtgatgcaag
     1681 ctcaggaaaa gattggcgag gcagggatga agggcatacg tgtgaggccg gtcccgggtc
     1741 cccgggcggg gcgggcgatc tgaatggaga gtatctaccg tgaatcgctg gagctccacc
     1801 ggaggatgcg ggggaagctc gaggtgcggg ggaaggtccc ggtccggacg aggagggacc
     1861 tctcccgggc gtacaccccc ggggtcgccc aggtctgccg cgagatccag aaaaaccgga
     1921 accgggcctg gatctacacc atcaaggcga acaccgtcgc catcgtctcc gacggctccg
     1981 cggtcctcgg cctcgggaac atcgggggct acgcggccat cccggtcatg gagggaaagg
     2041 ccctcctctt caaggacttc gccgggatcg acgccttccc catctgcttc gaggggtatc
     2101 acaccgactt catcgaggac gtgaagaaca tcgcccctgt cttcggcggg atcaacctgg
     2161 aggatatcgc cgcccccaag tgcttcgagg tcgaggaggc cctccaggac atcggcatcc
     2221 cggtgatgca cgacgaccag cacgggaccg ccgtcgtgat cctcgccgcc ttcctgaacg
     2281 cctgccgggt cacggggaag agcctctcgg acatcaccat cgccatgagc ggggcggggg
     2341 ccgccggctt cgcgaccttc cggctcctga agtgcctggg ctatgaccgg aaagcctgcc
     2401 tgcccgtcgg ggacatgatc atctgcgacc ggatggggat catccaccgg gaccggcccg
     2461 atctcctgaa gaacaagtac aagtacatca tcgcccacga aacgaaccgc gagggccgga
     2521 ccgggaccct cgccgatgcc atgaaggggg cggatgtctt catcggggtc tcggcccccc
     2581 gtatcgtgac cgggaagatg atccgatcca tgaaccccgg cgccgtggtc tttgccatgg
     2641 cgaacccggt ccccgaggtc atgcccgacg tcgcgaggaa ggcgggggcg gtggtggtcg
     2701 ggaccgggag gagcgacttc ccgaaccaga tcaacaacgt gctcgccttc cccggcatct
     2761 tccggggggc cctcgacgcc cgggcctcgc ggatcaccga cgacatgaag ctcgcagcgg
     2821 cccacgccct cgccgggatg gtgagggagc cgacccgggc gagggtcctc ccgggccccc
     2881 tgaaccggaa ggttgcatgg caggttgcgg cggctgtcga aaaggcggca aaggagtgcg
     2941 gctgcgccgt ggagatcggg taatccggag aggcatgctc cgagtctaca acaccatggc
     3001 ccggaggagg gagctcttcc tcccgaggtt ccccccggtc gtgaagttct tcagctgcgg
     3061 gccctccatc taccagcgcc cccacctcgg gaactaccgc accttcctgt acgaagacgt
     3121 cctcgagcgg tacctggaat accttgggta ccgggttgag cgggtcctcc ccttcacgga
     3181 cgtcgaggac aaggccatcg cccaggccga gaaggagggg gtgaccctct ctgaactcac
     3241 cgaccgggtg gccggggagt tcctcgccga ggcccggctc ctccgggtga agcccccgac
     3301 ctggaacccc cggtcctcca caacggtcga acacacggtg gagatcatcg aacggctcct
     3361 caggaaggga gtcgcctacc ggttcggccc cgacatcttc ttcgatcccc tgaagttccc
     3421 aaggttcggg aaacttttcc gcctggacat gacccggtgg ccccggacga ggcggaggtt
     3481 ccggaaggac acctatcccg gcgtgcagtg gaactacggc gacttcatcc tctggcacgg
     3541 ggcccgaagg ggggagaccg ccgtctggga cacgaggatc ggcaggggga gaccgtcctg
     3601 gaacgtccag gacccggcga tggccatccc cctcctcggg ccctccctcg acatccatgc
     3661 cggcggcatc gacaacctct ggcggcacca tgactacaac ctcgccgtgg ccgagtcctt
     3721 caccgggagg gagtacgccc gctactggat gcacggcgag cacctcctta tcggcgggaa
     3781 aaagatgtcg aagagccgcg ggaacatcgt ctacctctct gaccttacct cggacgggtg
     3841 cacccccgag gaggtccgct tcttcctcat ctacgggcac taccgcgacc ggatgaacat
     3901 gacccgggag aacctcgaga tggcgcagga ggtactcagg gagctcagga ctctcgccgg
     3961 acaggtagct gcgggcgcag gaaatccgac tgaaacgggg ccggcgggaa agcccgcttc
     4021 tccggacctg gaagaacggt tcagggagcg gatggacgac gacctggacg tgaagggggc
     4081 agtcgatggc atcgccggat ccctccaaga gctcgcggcc cgtcgcaggc ggggcaccct
     4141 cgccccggaa gaagcggcgg cagccctcac cacccttcac cggatcgatc aggttttcca
     4201 ggtgatcttt cccctggatc agaccgcatc ctccgggaac cggtgaccca tcgcccggcc
     4261 cttttccgac gggcgtccgg gcatggtaat gtaccatgcc cgacggccat caggtcgtct
     4321 ctgcgggatg cccgacggcc atcaggtcac ctccctgagg tgaccgacgt cctcgcgttc
     4381 acggtcctgc cgtgcccgac ggccatcagg tcacctccct gaggtgaccg acgtccgtcc
     4441 acaaaggttt tcaatcactt tcttaccgga gaaagaatgc ttatctcccg agaagatcat
     4501 acggtttgta gcgaaggcgg ggagggtaac ccccgcatcc gcccgagcag ggggtatatc
     4561 tttggcacca cggatcaatg cgcgtcacat cacctgtccc ggctgccagg aggatgtctt
     4621 catggaggag ctcgtgggcg ggcggtgccc gctctgcggc tgcgagctgg acgggagcga
     4681 ggagccgtgc gagggcttcg aggagctcct ggaccggtcc gaccttacct ggctcgtgta
     4741 ccagtacttc ctcttccgga aattcgaggc catgggggcg agcccgctcc agaccctcca
     4801 cctcatctcc cggttcgatg acccgggcgc caacggcccc tcctaccccg aggggacccc
     4861 tttctccgtc gagatccccc tctccatgct cgagaggatc cttcccaagc gctgcggccg
     4921 ctgcgggagg ctcttcttcg ccggagggag aaaactcgtg acgggcgacc tgggccgcac
     4981 cgccgaaaat acttcttata tctgcccgtc ctgctgattt cagaaggtgc gggcgaggag
     5041 ggtgggcgtc ccctccttcc cgcacacgat gcagttcttc gatatctccg ggagctggtt
     5101 gcccccgatg tccgtcccga ggatgctccc gccggtcttc ccctcgagga cctcggcgca
     5161 ttccctcgcc cggcaccagg agacaacggc cacccccttc ccgaccgcgg ccggcgcctc
     5221 ctcgagggtg aggggagcgg aaatccggtt ccgcatctcc gacacggccc tcgcaaggag
     5281 ggcccccgct agccccgaga ggacccctgc gacggaggat gccacggttt cccgggcggc
     5341 ctcggacttc tccccgagcc gcgtgacgag ggtcaccacc cttcgatcga tgtcccgtgg
     5401 gccgatctcg atccgaaggg gaacgccccg catctcccag tggtagtact tcgcgccagg
     5461 ccggaggtcc cgctcgtctg tctggacccg aaggcctgcg gcggcaaggt ccgacgcgag
     5521 actccgggcc gccccgagaa cctcgtcctt acgctttccg acgatgatgg ggatgatgat
     5581 cgcctggacg ggggcgacct ccggcgggag gacgagaccc cggtcgtccc cgtggacacc
     5641 gatggccgcc gcgatggacc gctccgagat cccgtagcag gtctggaagg catacctctg
     5701 atccccctgc tcgtcctcgt accggatccc gaaggtgcgg gagaagttgt ccccgaggtg
     5761 gtggacggta cctatctgga gcgtcctccc gtccggcatg atggtgtcga cggcgatggt
     5821 gtagtccgcc ccgggaaact tgtcccactc ggggcggcgg gtgacgagga ccgggatgga
     5881 gagccggtcg tagaaggcgc ggtagagccg gatggcctcc ttcgcctgct cctcggcatc
     5941 ctcccaggtc gcgtggacgg tgtgggcctc cttgaaggag ctgatctccc tcgcccggat
     6001 gaggggcctc gtggccttgg tctcgtaccg gaaggtgttc acgacctgat agagcctgag
     6061 gggaaggtct gcatgggacc gcacccagag ggcgtacatg gggtagatgg cggtttcgct
     6121 cgtgggccgg agggcgaggg gaacctcgag gggggactgc ccgccccggg tgacccagta
     6181 gacctcgttc tcgaagccct tgatgtgctc ggcctccttc atgaactcct gctcggggat
     6241 gagcaggggg aagagggcct cctggtggtc ccggtccatc agctcgcgga ggaccccgta
     6301 cacatgtttc ctgaaggcga agccgtaggg gtaccagacg tagaggcctt ttaccgggta
     6361 ccggttgtcg atgatccccg cccgccagag gatctcgtgg taccagcctg aaaagtcccc
     6421 cttccggggg agcggtcccg attcctcctc gacggccaac gatccacccc taggtcacca
     6481 gatgtatgat ctggggcgtg ataaatgcct cgataaacgc cgcaatggcc acgaggggca
     6541 cgatcacgag gaggaaggtg cgcccgtggg ccgccgccgt cgcggccgag tcccccccgc
     6601 ccctccactc ggcgaggagg gcgctcccga cgatgaaccc gagggaggcg gagaggatga
     6661 gggaggggat ctcgaagatc ccgtggggaa gcaccgccgc cgcgacgtag aggagacccc
     6721 tctcctcccg gacgatctcc atgatccccc cgatgatgac cccgttgctc gcgatgatgg
     6781 cgagggtgag gatcccgagg gtcgccccgc cgatgaagag gaggacgcag gcctgcaggt
     6841 tgttcgcgaa gatgaagatg gcgagggcga cgggctcccg ggcatccacc ttccccatca
     6901 cctggtccct gatgacctgg atgaactgct gcccggtaga ggggctctgc ctgacgaggg
     6961 cgaccccggc gaccgccgtg aggaggaagg ccgcgagggc caccaccgcc gcggtgcgga
     7021 gggggaggtc agagtcagac atgcagcacc tcctgccggc aggcggaggg cttcatgtca
     7081 tcatacatga agcaccatcc gcaccatgtc ccgcaccccg ggagcgagcc cgaccacgat
     7141 catcgcgagg aggacgaggt tcgcgaactc ccggtttccc tccctccggt acagttcgag
     7201 gacgtaaatg gccgggatga ccacgacgag cttcaggggg aagagggcaa acgcggtccc
     7261 ggcccaccgg atcagcgccg agccgaccac atggacctcc tggtacggga tggggccgaa
     7321 gtcgatgcca tatcccgtcg cagaggcatc gagcatctgc ccgatgacga ggagcctgaa
     7381 gaggggatcc gaggcgtact cccatctgaa gaccagctgg aggagggcca tgaccgcgag
     7441 agaggtcgcg gccgcaatgg cgaggatggc cgcgaggacc acgaaatcga tggtcgtccg
     7501 ggtgagaccg aaccaggcaa ggaggagggc gaccgccacc gaggccccca cgccgatccc
     7561 gccatagaag gtacggaacc ggggaacgag cctgtgcttc tcgagggtcc gggagaggac
     7621 gagagccgcg gctgcgatga agaaaacgac gaagaagatg aggggggtga tgagcaggta
     7681 cttcgcgggc ggcccgatca tccccgtgtc ctccaccacc cggaggagcc cgcccaggac
     7741 aacgaagggg atggtggcaa ggatgaacgg cccgtccagg gccacccccg accggcggag
     7801 ccagcggtac accaggtaca cggaaatgac gagaacgatg gcataggtgg cggtatccac
     7861 gatgttatag ggctggccct ggacaatgcc agcgatgtaa tatttattga tgaaatcccc
     7921 aatcatgatc aatcagaacc gg
//
LOCUS       DATGDI010000018         8498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_30939, whole genome shotgun sequence.
ACCESSION   DATGDI010000018 DATGDI010000000
VERSION     DATGDI010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8498
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30939"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VKO45_00745"
     CDS             complement(<1..200)
                     /locus_tag="VKO45_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04432.1"
                     /translation="MKSPKTVIVIMMIFLGIMVIFFTLSPEKPESGKLRLSDYPRLFG
                     EDTIIIIGKNASSAEADSAATIS"
     gene            complement(402..1403)
                     /gene="fen"
                     /locus_tag="VKO45_00750"
     CDS             complement(402..1403)
                     /gene="fen"
                     /locus_tag="VKO45_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HMA04433.1"
                     /translation="MGVDIRDILIPYKKAVGWETLTGVAAVDAHNALYQFLSIIRQPD
                     GTPLMDSRGRVTSHLSGILFRVLNFLELGIRPAFVFDGVPPEFKGATIAERKEIRREA
                     SVRWEEALARGEVAEARKHAQASSRVDEFVIASSKELLGLLGIPWVEAPSEGEAQAAH
                     MVAKGNATYAVSQDYDLLLFGAPVLVRNLTVSGKRKVRGRTITVSPEKLLLAEVLDGL
                     HLTREQLIQIGILVGTDFNTGVRGIGPKKAIQVVNEGKFGETLKEKVPDFDPAPVMEF
                     FMKPPVTDDYALTWKPVNAEGVVTMLCDGYDFSRERVDSALEKVRVTAGQKTLDRWF"
     gene            complement(1438..1752)
                     /locus_tag="VKO45_00755"
     CDS             complement(1438..1752)
                     /locus_tag="VKO45_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04434.1"
                     /translation="MEIRSSIPDVALTILLVISTLVLVMRLWQDVVIASAATLMMLSI
                     AGLFLLLGRKIQRLDASVVQRERTIRVNLEEISSGMAKRYDSTVDQVNGVIQELSRRM
                     YR"
     gene            1809..2732
                     /locus_tag="VKO45_00760"
     CDS             1809..2732
                     /locus_tag="VKO45_00760"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042500 - aspartic endopeptidase activity,
                     intramembrane cleaving [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presenilin family intramembrane aspartyl
                     protease PSH"
                     /protein_id="HMA04435.1"
                     /translation="MPLDLKNDLPLIAMPAMLLVVEAIALLLSRPLQAAGVAALPYPP
                     ESIWNTVIFLAMLLVFTLFILILIRFGVKRVLSAIIALSLFFTFYYIFASLALATAGE
                     TDLAGLATIVLSVGATAVLYKYPEWYVIDTLGILLSAGVASIFGIAVGIIPALALLIL
                     LAVYDAISVYRTKHMITLAEGVLSLKAPILFVIPKRRDYSFVKEGLAIKGEQGEAKER
                     GAFVIGMGDMIMPTILVVSANVFIQVAGWVVSPAALGAIAGSLVGLAVLLSYVKQGKP
                     QAGLPPLNAGTIAGFLIGCALAGAWGWVPAL"
     gene            complement(2683..3639)
                     /locus_tag="VKO45_00765"
     CDS             complement(2683..3639)
                     /locus_tag="VKO45_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HMA04436.1"
                     /translation="MDLEKIPTVPTAEEVMDRSLRRAASRRKEKKNKERADREFVMAV
                     AASLHDRLVKVIASFPDFEALPPLYRDLTEILFGLERIRMALGGVGWAAKHTRRIGGE
                     MSREIRWSADTRAVRRRAVARLSSIVHRVDKDLRFLNEVRNGLRVLPVLRDEFTVVVA
                     GYPNVGKSSFIRLVSSAEPQIASYPFTTKGIIVGHRKIGRSGIQFVDTPGLLDRPPEE
                     RSPIERQAVSALMHAARAVLFILDPSEMCGYPVDRQLALLEAVRGMVGVPVIVAVNKA
                     DVKGMEGYPNMSTLTGEGVEEVLEALLRERGPSPTPRPGRSR"
     gene            3688..4566
                     /locus_tag="VKO45_00770"
     CDS             3688..4566
                     /locus_tag="VKO45_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA04437.1"
                     /translation="MDLSLIQKEILITLITLYHQHSHAVKGEDIADVLKRNPGTVRNQ
                     MQALKAIGFVDGVPGPKGGYSPTALAYRELNLSNLDQEAAVPILRNGEPVAGMRVTEV
                     DFTTLCHPDLCHAMVRLAGSVKGFEIGEEVTIGPTPVNKLLIRGEIYGKDEVRQSILI
                     SISEMISLPKKAIRHYMSAPLITLSPGSSFRDAIHLFTDRHIHGAPVMSGGRLMGIVT
                     LTDIAKGLDDRLPMNTPVSSVMTSDVVYAEADTKLYEVVTRFKEQEIGRLIVVDNGRP
                     VGILTQSDVLGVFPTL"
     gene            4747..4998
                     /locus_tag="VKO45_00775"
     CDS             4747..4998
                     /locus_tag="VKO45_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HMA04438.1"
                     /translation="MSMILSRNLSRKKVMSNDGMVIGTLRNVMVDFDTGQVVDLIVVP
                     DPTFNTEGFRMDGDRMFIPFEAVKDIKDYIVVDRYLSKK"
     gene            complement(4980..5684)
                     /locus_tag="VKO45_00780"
     CDS             complement(4980..5684)
                     /locus_tag="VKO45_00780"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HMA04439.1"
                     /translation="MPTLRAVACDVDGTITDRERRISLTAIACIRDLVNRGVEVVLAS
                     GNTACFMDALARVIGTSGTVIAENGGVYRIGFSGELHTEGDRSVALAAYRELEEYYKK
                     RGITLDPYGEKYRFSDVAFARTVPSEEVRRVMQDREVKVLDTGFAIHLQARGINKGLA
                     LQRLAADIGIPIGEFLAVGDSENDLEMIEAAGIGVAVANARDEVKAASDYVTEKGDGE
                     GFAEAVKRYQPYFLER"
     gene            complement(5845..6819)
                     /gene="radA"
                     /locus_tag="VKO45_00785"
     CDS             complement(5845..6819)
                     /gene="radA"
                     /locus_tag="VKO45_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HMA04440.1"
                     /translation="MTGNGNDLEDLPGVGPTTADKLKEAGYTTIESIATASAAELAEV
                     AEISDAVAKKLVKAAKDRTVVSSFRTGADIMEQRKNVKKLRTLVPELDGLLGGGFETQ
                     AITELYGEFGSGKSQIVHQMAVNVQLPEEAGGLNGSCIFIDTENTFRPERIEQMVNGL
                     GIDVAPVQEFLDHIHVARAHTSDHQMLMVENARELANDLRDSERPVRLFIVDSLTAHF
                     RAEYAGRGTLAARQQKLNRHMHDLFKLLDEHNAVGLVTNQVLSNPAVFFGDPTKPIGG
                     NIVGHTATFRIYLRKSKGGKRIAKLVDSPNLPEGEAVFMVEQSGLKPA"
     gene            complement(7263..>8498)
                     /locus_tag="VKO45_00790"
     CDS             complement(7263..>8498)
                     /locus_tag="VKO45_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HMA04441.1"
                     /translation="DLLDEPTAAMIVIRELGRAHVKIRDLGSGSSLTSFFGKVISASS
                     PHEFQRSDGTTGQVADILLADETGQVRVVLWDEKAGAAAEVEVGEVLEVIAKPSPKGK
                     GEVSAMAFRKADCEIGCDMAPDRRFLPPEPAGEIEVKVIEVGKVRTFTRRDGSEGRMV
                     EAVIGNREETSRLVCWEPELLAGVKAGSPIRIRGATRSHGDEGNEYRLDEKGEVIPFE
                     GVIEVPFTDIGKVPGSGFVSVRGTIRSARPRRAFTTRRGQPSSVRNLTVSDGTGEMPL
                     VLWGERAALDIAEGDAVCLYRVQVRTGRDGGLELHAGRGSAVVLPPGEGGEEPVDLEG
                     TVIPVASGTCLEVAGECHPVEGELPVGRNVRVRGRRVRRTIIPAEVEVREPDPAGVRE
                     RLARFRASLLRTDPDHPGI"
BASE COUNT         1431 a         2884 c         2561 g         1622 t
ORIGIN      
        1 gatatcgttg ccgcactgtc cgcttctgct gaactggcat tcttcccaat gatgatgatg
       61 gtgtcttccc cgaacaggcg cggataatca ctgagcctca atttccctga ctcaggcttt
      121 tcgggtgaca gggtgaagaa gattaccatg ataccaagga agatcatcat gatgacgata
      181 acggtctttg gggatttcat acaccaacct gcaaatttac aattgatatt tcccaatctg
      241 ttctatatta atcttgactc ctaaaatact gaatgcaaat tccttctgaa cgtaccatgc
      301 cgataatctt aggacggccc gggggtgcaa aaaacctgat ctcatttttc ataacccgaa
      361 cttctcgttg ttccgctttg cagaaatccc aggtcagccc ttcaaaacca ccggtccagc
      421 gtcttctggc cggccgtgac ccgcaccttc tccagggccg agtccacccg ttcccgggag
      481 aagtcatacc cgtcgcacaa catcgttacg actccctcgg cattcaccgg cttccaggtg
      541 agggcatagt catccgtcac cggtggcttc atgaagaact ccatgaccgg cgcgggatcg
      601 aagtcgggta ccttttcctt cagggtctcc ccgaacttcc cctcgttcac gacctggatg
      661 gccttcttgg ggccgatccc cctgacgccc gtgttgaagt ccgtgcccac caggatcccg
      721 atctggatga gctgttcccg cgtgagatgg aggccgtcga gcacctccgc gaggaggagc
      781 ttctcggggc tcaccgtgat ggtccgtccc cgcaccttcc gtttcccgct cacggtgagg
      841 ttcctgacaa gcacgggagc cccgaagagg aggaggtcgt agtcctgcga gacggcgtag
      901 gtggcgttcc cctttgccac catgtgggcc gcctgggcct cgccctcgga gggggcctct
      961 acccagggga tgcccaggag accgaggagc tcctttgagg aggcgatgac gaactcgtcc
     1021 acccgggagg aggcctgggc atgcttccgg gcctcggcca cctcgcccct tgcgagggcc
     1081 tcctcccacc tcacgctggc ctcccggcgg atctccttcc gctcggcgat ggtcgccccc
     1141 ttgaactccg gcgggactcc atcgaagacg aaggccgggc ggatgccgag ctccaggaag
     1201 ttcagcaccc ggaagaggat cccggagagg tgggaggtga cccggccgcg ggagtccatg
     1261 aggggcgtcc cgtcgggctg cctgatgatg gagaggaact ggtagagggc gttgtgtgcg
     1321 tccaccgcgg ccacgccagt gagggtctcc cagcccacgg ccttcttgta cgggatgagg
     1381 atatcacgga tatcgacgcc caccgctcac ccctcctggt accggccggc cgtgcggtca
     1441 gcggtacatc cgcctggaga gttcctggat gacgccgttg acctggtcaa cggtgctgtc
     1501 gtacctcttc gccatccccg acgagatctc ctcgaggttc accctgatgg tccgctcgcg
     1561 ctggacgacg ctcgcatcga ggcgctggat cttccttccc agtaagagga agagccccgc
     1621 gatggagagc atcatgaggg tcgcggcaga ggcgatgacc acgtcctgcc agaggcgcat
     1681 cacgaggacg agggtggaga tgacgaggag gatggtgagg gcgacatccg gaatcgagct
     1741 tcggatctcc atgcttttaa tgttgaagtc ccattattta aaatacccct gagggaatgg
     1801 gtgaccggat gccccttgac ctgaagaacg acctccccct gatcgccatg ccggcgatgc
     1861 tcctcgtcgt cgaggcaatc gccctcctcc tctcccggcc cctccaggcc gcgggggtcg
     1921 cggccctccc ctacccgccg gagagcatct ggaacaccgt catcttcctc gccatgctcc
     1981 tcgtcttcac ccttttcatc ctcatcctca tccggttcgg ggtgaagcgc gtcctttcgg
     2041 ccatcatcgc cctctccctc tttttcacct tctactacat cttcgcctcc ctcgcgcttg
     2101 ccaccgcggg ggagaccgat ctcgccggcc ttgcgacgat tgtcctctcc gtcggtgcaa
     2161 cggcggtcct ctacaaatac cccgagtggt acgtcatcga taccctgggg atcctcctct
     2221 cggcgggagt ggcctccatc ttcgggatcg ccgtcgggat catccccgcc ctcgccctcc
     2281 tcatccttct cgcggtgtac gacgccatct cggtctaccg gacgaagcac atgatcaccc
     2341 tcgccgaggg ggtgctctcc ctgaaggcgc ccatcctctt cgtcatcccg aagcgccggg
     2401 actactcctt cgtgaaggag gggctcgcca tcaaggggga gcagggggag gcaaaggagc
     2461 ggggggcctt cgtcatcggg atgggggaca tgatcatgcc caccatcctc gtcgtctcgg
     2521 ccaacgtgtt catccaggtc gcgggctggg tcgtctcccc cgccgcccta ggggccatcg
     2581 ccggatccct ggtcggcctc gccgtcctcc tcagctacgt gaagcaggga aagccccagg
     2641 ccgggctccc cccgctgaac gccgggacca tcgcgggctt cctcatcggc tgcgccctgg
     2701 ccggggcgtg gggctgggtc cccgctctct gagcagggcc tcgaggacct cctcaacccc
     2761 ctctcccgtg agggtggaca tgttcgggta cccttccatg cccttcacgt ccgccttgtt
     2821 caccgcgacg atgacgggca ccccgaccat ccccctcacc gcctcgagga gagcgagctg
     2881 ccggtcgacg gggtagccgc acatctcgct cgggtcgagg atgaagagca ccgcccgggc
     2941 ggcgtgcatg agggccgaga cggcctgccg ctcgatgggg ctcctctcct ccggcggccg
     3001 gtccaggagc ccgggggtgt cgacaaactg gatcccggac cggcctattt tccggtgccc
     3061 gacgatgatc cccttcgtgg tgaaggggta ggaggcgatc tggggctctg ccgaggagac
     3121 gagccggatg aaggaggact tcccgacgtt ggggtacccg gcgaccacga cggtgaactc
     3181 gtcgcggagc accgggagca cccggaggcc gttcctcacc tcgttcagga accggaggtc
     3241 cttgtccacc cggtggacga tggaggagag ccgggccacc gccctccggc ggaccgcacg
     3301 ggtgtcggcg gaccaccgga tctcccggga catctcgccc cctatccgcc gggtgtgctt
     3361 ggcggcccac ccgactcccc cgagggccat ccggatcctc tcgaggccga agaggatctc
     3421 ggtgaggtcc cggtagaggg gggggagggc ctcgaagtcc gggaaggagg cgatgacctt
     3481 cacgagccgg tcgtggaggg aggccgcgac ggccatgacg aactcgcggt cagcgcgctc
     3541 cttgttcttc ttctccttcc gccgggaggc cgccctccgc agggaccggt ccatgacctc
     3601 ctcagcggtc ggcaccgtcg ggattttctc gagatccacg cctcacaacc tatgctttat
     3661 atatccaatt tcctatgatt aatcattatg gatctgtccc tcatccagaa agagatcctc
     3721 atcaccctca tcaccctcta tcaccagcac tcccatgccg tcaaagggga ggacatcgcc
     3781 gacgtcctga aacggaaccc gggcaccgtc aggaaccaga tgcaggcgtt aaaggccatc
     3841 gggttcgtgg acggggtccc cggccccaag gggggctaca gccccaccgc cctcgcctac
     3901 cgggagctga acctctcgaa cctcgaccag gaggcggcgg tacccatcct ccggaacggg
     3961 gaaccggtcg ccgggatgag ggtcaccgag gtggacttca ccaccctctg ccacccggac
     4021 ctctgccatg ccatggtcag gctcgcgggc tcggtgaagg gcttcgagat cggcgaggag
     4081 gtcaccatcg ggccgacgcc cgtgaacaag ctcctcatcc ggggcgagat ctacgggaag
     4141 gacgaggtgc ggcagtccat cctcatctcc atctccgaga tgatctccct cccgaagaag
     4201 gccatccgcc actacatgag cgcccccctc atcaccctct cccccggctc gagcttcagg
     4261 gacgcgatcc atctcttcac cgaccggcac atccacgggg cccccgttat gtccgggggg
     4321 aggctcatgg ggattgtcac cctcaccgac atcgcgaagg gccttgacga ccggctcccg
     4381 atgaacacgc cggtctcctc ggtgatgacg agcgacgtgg tgtacgcgga ggccgacacg
     4441 aagctctacg aggtggtgac ccggttcaag gagcaggaga tcgggaggct catcgtggtc
     4501 gacaacggga ggccggtcgg catcctgacc cagtctgatg tcctcggggt cttcccgacc
     4561 ctctgaggcg ccccaccgca ccgtgccagg aatgcgacgg gacccgaaac cggggccgtt
     4621 tcgggaatgc tttttacccc gggcgggtga acaaaccccg attatggttc agaatgtcct
     4681 aaattcctga tttcgcccgt acgtcctaga gaacaattta agtattccaa agttctcctt
     4741 ttttatatga gtatgatttt atcgcgcaac ctctcccgaa aaaaggtcat gtccaatgac
     4801 gggatggtca tcggcacatt gaggaacgtg atggtggact tcgataccgg ccaggtggtg
     4861 gacctcatcg tcgtaccgga cccgaccttc aacaccgagg gattccggat ggacggggac
     4921 cggatgttca tccccttcga ggccgtaaag gacatcaagg actatatcgt cgtcgaccgc
     4981 tacctctcca agaaataagg ctgataccgc ttcaccgcct cggcaaagcc ctccccgtcc
     5041 cctttttccg tgacatagtc cgaggcggcc ttcacctcgt cccttgcgtt cgccacggcc
     5101 accccgatcc ccgccgcctc gatcatctcc agatcgttct cggaatcgcc caccgcgagg
     5161 aactccccga tggggatccc gatgtctgcg gcgagcctct gcagggcgag ccccttgttg
     5221 atcccccgtg cctggaggtg gatggcgaac ccggtgtcga ggacctttac ctcccggtcc
     5281 tgcatcaccc tccgcacctc ctccgacggg accgtgcggg cgaaggcgac gtcggagaag
     5341 cggtacttct cgccataggg atccagggtg atgcccctct ttttgtagta ctcctcgagc
     5401 tcccggtacg cggcgagggc cacgctccgg tcaccctcgg tgtgaagctc tcccgaaaag
     5461 ccgatccggt agaccccgcc gttctcggcg atgaccgtcc ccgaggtccc gatgacccgg
     5521 gcgagggcgt ccatgaagca ggccgtgttc ccgctcgcaa ggaccacctc cacccccctg
     5581 ttcacgagat cccggatgca ggcgatggcg gtgaggctga tccggcgctc gcggtctgtg
     5641 atggtcccgt ccacatcaca ggcaactgcc ctcagggtcg gcacgctccg gtactggatc
     5701 aggacaggta aaaaaaggag gtgttccggg cccggcatcc cccagccagg cccgggacct
     5761 tctggcgggt ttccaggcct caggcgggct tcaggccgta ctcaaaccgg tcaggccggt
     5821 ttgaggccgt actcaaggcg gccatcaggc cggcttgagg cccgactgct ccaccatgaa
     5881 gaccgcctcg ccctcgggca ggttcgggct gtccacgagc ttcgcgatcc gcttcccgcc
     5941 cttgctcttc cggaggtaga tcctgaaggt cgcggtgtgg ccgacgatgt tcccgccaat
     6001 gggtttcgtc gggtcgccga agaagaccgc cgggttggag aggacctggt tcgtaacgag
     6061 gccgacggca ttgtgctcgt ccaggagctt gaagaggtcg tgcatgtgcc ggttcagctt
     6121 ctgctgccgg gccgcgaggg tcccccgccc ggcgtactcg gcccggaagt gggcggtgag
     6181 ggagtcgacg atgaagagcc gcaccggcct ctcgctgtcc ctgagatcgt tcgcgagctc
     6241 ccgggcgttc tcgaccatga gcatctggtg gtcggaggtg tgggcccggg cgacatggat
     6301 atggtcaagg aactcctgga caggggcgac gtcgatgccg aggccgttca ccatctgctc
     6361 gatcctctcg gggcggaagg tgttctcggt gtcgatgaag atgcaggagc cgttgaggcc
     6421 ccccgcctcc tcgggaagct ggacgttcac cgccatctgg tggacgatct ggctcttccc
     6481 cgacccgaac tccccgtaga gctcggtgat ggcctgggtc tcgaagcccc caccgaggag
     6541 gccgtcgagc tcgggaacga gggtccggag cttcttcacg ttcttccgct gctccatgat
     6601 gtccgccccg gtccggaagg agctcaccac ggtccggtcc ttcgcggcct tcacgagctt
     6661 cttcgcaacg gcgtcgctga tctcggccac ctcggcgagc tccgccgccg atgcggtggc
     6721 gatgctctct atggttgtgt accctgcctc tttcagcttg tcggccgtcg tcggcccgac
     6781 acccggcaga tcttccagat cgtttccgtt tcctgtcatt gccatctccc ccaggggggt
     6841 ccggttacac ttccccgttc cccggatccc tttatagagg tggggcgggc gccgtgaaag
     6901 taatatctcc atatggatcg cgaaggtggt ttatttagtg aaccaaagga ttgaagctcc
     6961 tgtaaaacgc agattctcaa actatctctt gatcggaccg aattgtcgac aggcggacgc
     7021 tcctgggggt tcgctccgct cacccccgcc gctgtggcgg cccccagagg cgatgggctt
     7081 ttcatcccgt tggaccaccg aatcaggtag ctgcggaaac ggagggggtg cccgtgcggc
     7141 ggggggtggc gtagcccccc ccccgagagc accccaataa aaagatcacc atgatgtcca
     7201 gtttgattgg tccttaatca gaacatctcc aggacggaat agctctagag tccgaacgat
     7261 tctcagattc ctgggtgatc aggatctgtc ctgaggaggg atgcccggaa ccgggcgagg
     7321 cgttccctca cgccggcggg gtccggctcc cgcacctcta cttcggcggg gatgatggtc
     7381 cgcctcaccc gcctcccccg gacccgcacg ttccgtccga cggggagctc gccctctacg
     7441 ggatgacact ctccggccac ctccaggcat gtgccggagg ccacgggaat gacggtgccc
     7501 tccagatcca ccggttcctc cccaccctct ccggggggca ggaccacggc gcttccccgg
     7561 cccgcgtgga gctcgagccc cccgtccctg cccgtgcgga cctggacccg gtagaggcac
     7621 acggcatccc cctcagctat atcgagggcc gccctctctc cccagaggac gaggggcatc
     7681 tccccggtcc cgtccgagac cgtgaggttc cggaccgagg agggctggcc ccggcgggtg
     7741 gtgaaggccc tcctaggacg ggcggaccgg atcgtccccc ggaccgagac gaatcccgac
     7801 ccggggacct tcccgatgtc tgtgaagggg acctcgatca ctccctcaaa gggaatcacc
     7861 tcccccttct cgtccaatcg gtactcgttc ccctcgtccc cgtgggagcg ggtggccccc
     7921 cggatccgta tcggggatcc ggccttcact cccgcgagga gctccggctc ccagcagacc
     7981 agtcgggatg tctcttcacg gttcccgatg accgcctcca ccatccggcc ttcggacccg
     8041 tccctgcggg taaaggtccg gaccttcccg acctcgatga ccttcacctc gatctctccg
     8101 gcgggttcgg gcgggaggaa cctccggtcg ggggccatgt cgcagccgat ctcgcagtcc
     8161 gccttccgga aggccatggc gcttacctcc cccttcccct ttggcgaggg tttcgcgatg
     8221 acctcgagca cctccccgac ctcgacctcg gcggccgcgc cggccttctc gtcccagaga
     8281 accacccgca cctggccggt ctcgtccgca aggagaatgt ccgcgacctg gccggtggtc
     8341 ccgtcgctcc tctggaactc gtggggcgag cttgcggaga tgaccttccc gaagaaggag
     8401 gtgaggctgc ttcctgaccc gaggtcccgg atcttcacgt gggccctccc gagctcccgg
     8461 atgacgatca tcgccgcggt gggttcgtcg aggaggtc
//
LOCUS       DATGDI010000019         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_205637, whole genome shotgun sequence.
ACCESSION   DATGDI010000019 DATGDI010000000
VERSION     DATGDI010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205637"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VKO45_00795"
     CDS             complement(<1..513)
                     /locus_tag="VKO45_00795"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HMA04442.1"
                     /translation="MNTITLTSKAKPELFLEADNITPDRFAGKTNDEIAALHLYEGST
                     QHALGKFLDVSGSSGPTAAETRIVIRGDVTRLKYVGMKMTAGEILIEGNADMYVGAWM
                     QGGKIQVKGNVDAFCGTGMKGGEISIEGDAGNYLGAAYRGDWRGMRGGIIRVKGNAGS
                     DMGYFMNGGTI"
     gene            complement(526..2229)
                     /locus_tag="VKO45_00800"
     CDS             complement(526..2229)
                     /locus_tag="VKO45_00800"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HMA04443.1"
                     /translation="MAEYLIKNGFVFDPTQKIKGDVKDVAVKDGKIVEKVSNPKVIDA
                     KGMTVMAGALEIHAHVAGPKVNIGRIYRPEDKLFTFRPTEGRHRMEGGFSIPTTYKTG
                     YTYARLGYTTVMEAAMPPLFARHVHEEMRDTPIIDEGAYPVFGNNWFVLEYLKNKELE
                     NVAAYCAWLLRVTKGFAIKVVNPGGTEAWGWGLNCLQVNDPVPYFDVTPEQIVKGLIE
                     ANEHLGLPHSMHIHGNNLGNPGNYTTTLDTLKLASGFKAKNKFGREQVMHHTHIQFHS
                     YGGTKWADFESKARPIADYVNKHKNITIDVGFVTLDETTTMTADGPFEHHLTELNHLK
                     WANADVELETAAGVVPYIYSPDIFVSGIQWAVGLEIALLSKDPMRCYITTDHPNAGPF
                     TRYPRIIKWLMSAKAREEQIKAFKQSDKVVNASEIANIDRELTLYEIAQMTRAGPAKS
                     LGLAGMYGGLKPGLDADVVVYDFNPEKEHKPMEIEEAFSEAKYLLKTGVPIIMDRKFV
                     SEGKKRTLWVDAKVNENAQVMRDVKEKFMKFYTVTEKNYEVTGRHFIPNPYAIEVDAT
                     H"
     gene            complement(2230..>2505)
                     /locus_tag="VKO45_00805"
     CDS             complement(2230..>2505)
                     /locus_tag="VKO45_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HMA04444.1"
                     /translation="DPGAHFPISSVKKIAKLPSVAVDPHFTPTTEICKLHVPVAFVGI
                     ECGGNAYRMDNVPIDTRKVVEPPEGMLTDEQFLEKVLHRIREIKGAA"
BASE COUNT          379 a          787 c          783 g          556 t
ORIGIN      
        1 gatggtaccg ccgttcatga agtagcccat gtcgctcccg gcgttcccct tcacccggat
       61 gatgccgccc cgcatgccac gccagtcgcc gcggtaggcg gcccccaggt agttcccggc
      121 atccccttcg atggagatct caccgccctt catgccggtg ccgcagaagg catccacgtt
      181 ccccttgacc tggatcttcc cgccctgcat ccaggccccg acgtacatgt cggcattccc
      241 ctcgatgagg atctcgcccg ccgtcatctt catcccgacg tacttgagcc gggtgacatc
      301 gccccggatg acgatccggg tctcggcggc ggtcggaccc gaggatccgg agacatcgag
      361 gaatttcccg agggcatgct gggtgctgcc ctcgtagagg tggagggccg cgatctcgtc
      421 gttcgtcttt cccgcgaacc ggtcgggggt gatgttatcg gcctcgagga agagctcggg
      481 tttcgccttt gaggtgagtg tgatggtgtt catcgttcgt gcctcctagt gggttgcgtc
      541 cacctcgatg gcgtacgggt tcgggatgaa gtgcctcccg gtcacctcgt agttcttctc
      601 ggtgaccgtg tagaacttca tgaacttctc cttcacgtcc cgcatgacct gggcgttctc
      661 gttcaccttg gcatccaccc agagggtccg cttcttccct tcggagacga acttccggtc
      721 catgatgatg gggacgcccg tcttcaggag gtatttcgcc tccgagaagg cctcctcgat
      781 ctccatgggc ttgtgctcct tctccgggtt gaagtcatag accaccacat cggcatcgag
      841 accgggcttc aggccgccgt acatcccggc gagcccgagg ctctttgcag gccccgccct
      901 cgtcatctgg gcgatctcgt agagggtgag ctcccggtcg atgtttgcga tctccgaggc
      961 gttcacgacc ttgtcgctct gcttgaaggc cttgatctgc tcctcccggg cctttgcaga
     1021 catgagccac ttgatgatcc gcgggtaccg ggtgaagggt cccgcattcg ggtgatcggt
     1081 cgtgatgtag caccgcatgg ggtccttgga gaggagggcg atctcgaggc ccacggccca
     1141 ctggatcccg ctgacaaaga tgtccgggct gtagatgtag gggacgaccc ctgccgcggt
     1201 ctcgagctcc acgtcggcat tcgcccactt caggtggttg agctcggtga ggtggtgctc
     1261 gaacggaccg tcggccgtca tcgttgtcgt ctcgtccagc gtgacgaacc cgacatcgat
     1321 ggtgatgttc ttgtgcttgt tcacgtaatc ggcgatgggc cgggccttgg actcgaagtc
     1381 cgcccacttc gtcccgccat aggagtggaa ctggatgtgg gtgtggtgca tcacctgctc
     1441 gcggccgaac ttgttcttgg ccttgaagcc gctcgcgagc ttcagggtgt cgagggtcgt
     1501 ggtgtagttc cccgggttcc ccaggttgtt cccgtggatg tgcatggagt ggggaaggcc
     1561 caggtgctcg ttcgcctcga tgagcccctt cacgatctgc tcgggggtga catcgaagta
     1621 ggggaccggg tcgttcacct ggaggcagtt gaggccccag ccccaggcct cggttccgcc
     1681 cggattcacg accttgatgg caaagccctt cgtcacgcgg aggagccagg cacagtaggc
     1741 cgcgacgttc tcgagctcct tgttcttgag gtactcgagg acgaaccagt tgttcccgaa
     1801 gaccgggtac gccccctcgt caatgatggg ggtgtcccgc atctcctcgt ggacatgccg
     1861 ggcaaagagg ggcggcatgg ccgcctccat gaccgtcgtg tacccgagcc gggcataggt
     1921 atacccggtc ttgtacgtgg tggggatgga gaacccgccc tccatccggt gcctgccctc
     1981 cgtcgggcgg aaggtgaaga gcttatcctc gggccggtag atccggccga tgttcacctt
     2041 cgggcccgcg acatgggcgt ggatctcgag ggcccccgcc atgacggtca tgccctttgc
     2101 gtcgatgacc ttgggattcg ataccttctc gacgatcttc ccgtccttca ccgcgacgtc
     2161 cttcacgtcc cccttgatct tctgggtggg gtcaaagacg aacccgttct tgatgaggta
     2221 ctcggccatt tacgccgccc ccttgatctc ccggatccgg tgcaatacct tctccaggaa
     2281 ctgctcgtcg gtgagcatgc cctcgggagg ctccacgacc ttccgggtgt ctatggggac
     2341 gttgtccatg cgataggcgt tccccccgca ctcgatcccg acgaaggcca ccggtacgtg
     2401 cagcttgcag atctcggtgg tgggagtgaa gtgagggtca acggccaccg aggggagctt
     2461 cgcgatcttt ttcaccgagc tgatcgggaa gtgcgccccg gggtc
//
LOCUS       DATGDI010000020        12250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_351048, whole genome shotgun sequence.
ACCESSION   DATGDI010000020 DATGDI010000000
VERSION     DATGDI010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 12250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12250
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351048"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VKO45_00810"
     CDS             <1..952
                     /locus_tag="VKO45_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HMA04445.1"
                     /translation="KEDPTVKVSINEETGEHLISGMGELHLEIITHRIKRDKGVEIVT
                     SEPIVVYRETVSGAASDVEGKSPNRHNRFYLDLSPLEPAVVAAIRNGEISMNMMEIER
                     RTVLQKAGWEKDEAKGIKDIEGTCVFLDMTKGVQYLNETMELILEGFHEAVAGGPLAD
                     EPIMNLKVKLTDVKLHEDAIHRGPAQVIPAVRAAVKAGMLIAGDVLMEPYQKIQINVP
                     QEQMGAATSQIQGRRGQVFDMQTEGDQFVVTGKAPVAELFGFASELRSATEGRAMWST
                     EFAGFDVVPANILKDVVMAIRKRKGLKLEMPRPTDYIDKV"
     gene            complement(997..1470)
                     /locus_tag="VKO45_00815"
     CDS             complement(997..1470)
                     /locus_tag="VKO45_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HMA04446.1"
                     /translation="MKTIVAFYSWQGHTGKVAKALATRIGADLVQIEPEKESGMFGKA
                     MGAILGRAAPIRPCKTDLSGVDFLVLASPVWAGKVPPYINRYIALLSNTSGKPFSLVA
                     EMGKSGAEKAIALMRKRLEAKGMQFVSSTFTLESEVDAGSFVPKIEEFAKTIVAK"
     gene            complement(1467..1961)
                     /locus_tag="VKO45_00820"
     CDS             complement(1467..1961)
                     /locus_tag="VKO45_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04447.1"
                     /translation="MASKLAACAILILSALILAGGCTSPVIPQGGGSTPGGTQPPPGT
                     QPPSGNQGTIFPGPVVTIPPDFNVEIQENRNHNPVYPYITVTFRGGKGQIFVQRILIT
                     MVPSVGQPVTQEIVRPEGGQISVGEYRRFTGTTGVDRVIVVVTILGKDYKIIDMLDDY
                     NLHP"
     gene            complement(2041..2532)
                     /locus_tag="VKO45_00825"
     CDS             complement(2041..2532)
                     /locus_tag="VKO45_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04448.1"
                     /translation="MIRKITPFLVLLLIALASVPGCTAPQGDGGTPTTTTTTMTATPV
                     PGVTIPPGPVVTPLPNYAVAVQVTRNPNTAFPYITVTFRGGYGQILLRRLTVTVARSD
                     GKVIQEVIPQSGTQQYAIGDFVRITGTTGTDEVAVVANINGKDYKIYDQNLDFYTIPP
                     PPS"
     gene            complement(2611..3246)
                     /locus_tag="VKO45_00830"
     CDS             complement(2611..3246)
                     /locus_tag="VKO45_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04449.1"
                     /translation="MTPRSTVPILRLILSVLTIVALAVPGSAAPIRAIPGEDIPLGGT
                     ATGVDVVYLFLTGPNLPDPGISLTGGTPVSTGVPSSFTRVEINTDGTWRFTWQTGSIG
                     RTLDPGTYTVYAALEPVSRQDLAGADYTEQAVILGPPVGTVTVTVTVPAATGSPVVST
                     TTILPTVTLTTPSPVESTPLVTAPAPGRLGPGALIPFGASALAILAFRRRR"
     gene            complement(3419..3832)
                     /locus_tag="VKO45_00835"
     CDS             complement(3419..3832)
                     /locus_tag="VKO45_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMA04450.1"
                     /translation="MLDHRMPGEVFRKGESSDRDRGDKVGVLPISPPNDADYLYEVSL
                     ELSDAGKFESAIDLLTKALILRPQFPVAWYQKGVCQVQLDKVDDALASYDTCLNHDPY
                     HAEAWFNRGMLLKKQGQHEEGDRSVKRAIDLCLGR"
     gene            4282..9099
                     /locus_tag="VKO45_00840"
     CDS             4282..9099
                     /locus_tag="VKO45_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMA04451.1"
                     /translation="MKIIDQEKMERIKALLKTHPRGLSITNLASITGLNRNLIAKYLD
                     MLVISGQVEIQQYGPAKVYFLSQRIPVSAMIEITSDLVMVLDQDRKIVHVNKPFTRFL
                     AMKRENLLGKTIGEVGHPFLSSIPLPESSSPLDTGDGGTGEVRYGSATGDTFFRIKRA
                     PAVFEDGAVGLTLIIEDITEQKKYEETLRLSEARYRGIVQDQTEFINRYLPDTTLTFV
                     NHAICLFANRPPGDLLGKRFIDMIPEEERGRVRQNIGALTPENPTATIEHRMADGRGR
                     VRWLHWTNRAIFDEKGTILEYQGMGRDITDQRETAERISRYILQLEFISRKAVEFVHS
                     ASGDDIFLTIALGVKSLCPEAGVCVLSSEGEGVMKVAAVPDDEMRDRIRSILGKDLVG
                     TRISLSADIMEKVRDGILHRVPGDLYTQCIPPPSPDEQVRLKELFGNVDNYIMGLTHG
                     KDLFGGVFLMFREGVLLQNEHLLVTYLNQASVAVQRQIYQEELQRSREEYRRIVETSA
                     EGIWVLDRNYRSTFLNPRLAEMLGYEPGELAGRQIEEFMLPEDIAAHRLRVAERRSGA
                     SGHFEQRFRKKDGSLIWVMANATPLFESGEFTGAFAMLTDVTTEKQAGDALRESEERY
                     RDLTEASFDIIFTIDREDRLAYANRRCLEFLGRRYEEVVGKPRASLFTPEISEKMKEN
                     LVKIFSTGEPLLDSANPVVLGDREFWLDTNLIPLKNADGSVNLVMGISRDITLRKKTE
                     DALRESEEKYRALVEQAHDMIVVVQDGIIKFCNQKVAEMWGGDIREIIGQPYERFIDP
                     EELPRVRENYLRRMAGEAAPDLYDTLLLRRDGSRVHVDLSAGTIVFDGRPADLIVLRD
                     ITEKKETEEQLKCTYELQKAILDASPVGIGYVSNRTLTWANKAMYRIQGVEPLELRGQ
                     HERIVFSDEKEYERVCGQVYHEIGETGFSELETALKRKDGSLVPVSLRAAPIRPGSTD
                     LIILVTDITARKKAEEELRKTHALQQAILAASPMGIGVTANRILIWANEAMSRIHGYG
                     PGEREGQSAQLLYEDEREYERVTREISLAMETGEFRVLETVWRRKDGSRANIRLTVGP
                     VRPGSPEVLSVARDITAQKKAEEELGKTRALKDAILSSSPVGIGVSRNRFLIWENDAM
                     ASICGIEKDELEGMPVRAVYESDEEYERITRVGSEAIGSGHFRETETVWVRKDGSSVD
                     VQLSLGPVAPGSSEILAIAQDITARKKAAEQLRQTQALQEAILRASPVGIGLISERTL
                     QWENDAMCEILGIREGELKGQPLDTVFIDEQEYGRTSRVLDQAIRQNGSGEVETVWKR
                     KDGSRVEVSLRVAPVRPGSPEVIGVATDITDRNRAGRALRESEETFRTMVTINPLPLS
                     LIDAKGKYLHVNPSFTRLFGYTLEDIPDGKAWFALAYPDEETREVAIRYWKADAAEHP
                     VGEVRPRTFKVRCKDGSFKDILFLPASTPAGLQVVVYQDLTRETMTERLSDSEDRYRH
                     LVDDLNVGIYRSSGDTEGRFIWGNTGLLSILGYEDLADLRGVPVRDIFQKPGGRKALL
                     RELQEKKFVKNRLLSLKKKDGTPVKVSVTALAEFDEKGKLQFITGLVQEVPRD"
     gene            9410..11470
                     /gene="fdhF"
                     /locus_tag="VKO45_00845"
                     /pseudo
     CDS             9410..11470
                     /gene="fdhF"
                     /locus_tag="VKO45_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313753.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            11470..>12250
                     /locus_tag="VKO45_00850"
     CDS             11470..>12250
                     /locus_tag="VKO45_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HMA04452.1"
                     /translation="MALFGGKTAGVKKGDMVYAWTTDSELAKRAECGGAVTTFLRQAL
                     EQKLVDAVLAVRRGQDIYDAVPTLITDPKELTKTAGSLHCGTLLLSKVLAKHLKGAKD
                     RKIALTVKGCDLMGILELAKRNAINRDNLYIVGVNCGGTVSPIKAMKMIQEVYQVDPM
                     KVHKEEIDKGQFIIEYEGGHKGISMDELEEQGYGRRSNCRRCKLKVPRQADLACGNWG
                     VIGEKAGKATFVEVCSEKGANLVNGAVKAGILATEAANPKGI"
BASE COUNT         2573 a         3657 c         3920 g         2100 t
ORIGIN      
        1 caaggaggac ccgacggtga aggtctccat caacgaggag acgggggagc acctgatcag
       61 cggcatgggg gagctccacc tggagatcat cacccaccgg atcaagcggg acaagggggt
      121 cgagatcgtc acctccgagc ctattgttgt gtaccgggag accgtgtcgg gagcagcgag
      181 cgatgtggaa gggaagtccc cgaaccggca caaccggttc taccttgacc tctcgcccct
      241 tgagcccgcc gtggtggcgg ccatccggaa cggcgagatc tccatgaaca tgatggagat
      301 cgagaggagg accgtcctcc agaaggcagg ctgggagaag gacgaggcaa aggggatcaa
      361 ggacatcgag ggaacctgtg tcttccttga catgacgaag ggcgtccagt acctgaacga
      421 gaccatggag ctcatcctcg agggctttca cgaggcggtg gcgggcggcc ccctcgccga
      481 cgagcccatc atgaacctca aggtgaagct caccgatgtg aagctccacg aggacgccat
      541 ccaccggggc cctgcccagg tcatcccggc ggtccgggct gccgtgaagg cgggcatgct
      601 catcgcaggg gacgtgctca tggagcccta ccagaagatc cagatcaacg tgccccagga
      661 gcagatgggc gctgcaacct cccagatcca gggccggcgc gggcaggtct ttgacatgca
      721 gaccgagggt gaccagttcg tggtcaccgg gaaggccccc gtcgccgagc tcttcggctt
      781 tgcgagcgag ctccggtccg ccaccgaggg ccgggccatg tggtccaccg agttcgcggg
      841 cttcgacgtg gtcccggcga acatcctgaa agatgtggtc atggccatcc ggaagcggaa
      901 gggcctcaag ctcgagatgc cccggcccac tgactacatc gacaaggtct gaaaaatctt
      961 atttttacgg gtccggaggg ccggaccccg aatttactat ttcgctacaa tcgtcttcgc
     1021 gaactcctcg atcttcggca cgaaggaacc cgcgtccacc tcggactcca gggtgaaggt
     1081 cgaggagacg aactgcatcc ccttcgcctc caggcgcttc ctcatgaggg cgatggcctt
     1141 ctcggccccg ctcttcccca tctctgcgac aagggaaaaa ggcttcccgg aggtgttcga
     1201 gaggagcgcg atgtaccggt tgatgtaggg cgggaccttc cctgcccaga ccgggcttgc
     1261 gaggacgagg aagtccaccc ccgagaggtc ggtcttgcat ggccggatgg gggccgccct
     1321 cccgagtatc gctcccatgg ccttcccgaa catccccgac tccttctcgg gttcgatctg
     1381 cacgagatcc gccccgatcc gggtggcgag ggccttcgcc accttcccgg tgtggccctg
     1441 ccagctgtag aacgcgacaa tggtcttcat gggtgcaggt tgtaatcgtc gagcatatcg
     1501 atgatcttat agtccttccc gaggatggtg acgacaacga tgacccggtc gacacccgtg
     1561 gtcccggtga agcggcgata ctccccgacc gagatttggc cgccttcggg ccggactatc
     1621 tcctgcgtga ctggttgccc gacagacgga accatggtga tcaggatcct ctggacgaag
     1681 atctgtccct ttccgccccg gaaggtaacg gtgatataag ggtagacggg attgtggttc
     1741 cggttttcct gaatctcgac attgaaatcc ggaggaatcg tgaccacggg acccgggaag
     1801 atggtgccct ggttccctga cgggggttgg gtcccgggcg gtggctgggt cccccccggg
     1861 gttgacccgc cgccctgcgg gatgacgggc gaggtgcacc cccctgccag gatgagggca
     1921 gagaggatga ggatcgcaca ggctgcaagc ttcgatgcca tacgggtgtt ttgtcccgaa
     1981 agggggataa tcattcccct gtgaaaaggg ggagcctttc ccccgggttg ggggaaggga
     2041 tcaggagggt ggaggaggga tggtgtaaaa atccaggttc tggtcgtaga tcttgtaatc
     2101 cttcccgttg atgttggcga ccaccgcgac ctcgtccgtt cccgtggtac cggtgatcct
     2161 cacaaagtcg ccaatcgcgt actgttgcgt acccgattgc gggatgacct cctggattac
     2221 cttgccgtcg gaccgggcca ccgtgaccgt gagtctcctt aggagaatct gcccgtaccc
     2281 tccccggaag gtcaccgtga tgtagggaaa ggcggtgttc ggattccggg tcacctggac
     2341 ggcaaccgcg tagttcggaa gcggggtcac caccgggccg ggcgggatgg tcacgcccgg
     2401 caccggggtt gcggtcatcg tggtcgtggt cgtggtcggg gttcccccat ccccctgcgg
     2461 ggccgtgcag cccggcacgg aggccagggc gatgaggagc agtacgagga agggagtgat
     2521 cttacgtatc atagggagcg atgacgcgtt gaaataataa agattccttg gggtcaggcc
     2581 aggggagatc cggcccccca ccctcccgcc tcacctcctc cgacggaatg cgaggatcgc
     2641 gagggcagag gcgccgaagg ggatgagggc gccggggcca agccggccgg gggccggggc
     2701 cgtgaccagc ggggtggatt ccaccgggga tggggtggtc aaggtgacag tcggaaggat
     2761 tgttgttgtg ctcaccacgg gggaaccggt ggccgcggga accgtcaccg tcaccgtcac
     2821 ggtcccgacc ggcggcccga gaataactgc ctgctctgtg taatcggccc cggccagatc
     2881 ctggcgggac accggttcga gggcggcgta cacggtgtac gtccccgggt cgagagtccg
     2941 gccgatggag cccgtctgcc aggtgaaccg ccatgtcccg tccgtgttta tctcgaccct
     3001 ggtaaagctc gacgggacac cggtcgacac gggagtaccg ccggtaaggc tgatgcccgg
     3061 gtcgggcagg ttcggccccg tcaggaagag gtacacgaca tcgacccccg tcgcggtccc
     3121 cccgaggggg atgtcctctc ccgggatcgc acggatgggg gcggcactcc cggggaccgc
     3181 cagggccacg atggtcagga ccgaaaggat cagacggagg atggggacgg tggatctcgg
     3241 agtcatggca aggaggtcat agggggagag gagaaaaagc tgcccgtggg agtactcgca
     3301 gaaaggggct aacccgacaa cggatgataa aaacgggggg gatcccggcg acggatcccc
     3361 cgagtctctc caacctgttc caaagtgtgc ctttgaatga ttgaacccgg gtccggatct
     3421 accttcccag gcagaggtct atcgctctct tcaccgatct gtccccttcc tcatgttgcc
     3481 cctgcttctt gaggagcatt ccccggttga accatgcctc ggcatggtac gggtcatggt
     3541 tgaggcaggt atcgtagctg gcgagggcat cgtccacctt gtccagttga acctggcaga
     3601 ctcccttctg gtaccatgcc acggggaact gcggcctgag gatcagcgcc ttggtcagca
     3661 gatcgatggc ggactcgaat ttccctgcat ccgacagctc aaggctgacc tcgtagaggt
     3721 aatccgcatc gttcggcggt gatatgggta gaaccccgac cttgtcaccg cgatccctgt
     3781 cagaggattc ccccttccgg aagacctccc cgggcatcct gtgatcgagc attttccctc
     3841 ccttctccct tccaactccc acagacgagc tctcgaacac acacctgtgg gcaggagtag
     3901 gcaggaaagg ctggaaaata tatttttgct cccgatctct gcacgaaata aacgaaatac
     3961 ggttattcct gcggttctgc catgcacatg ttccatcaga gagtttccga ggttttatat
     4021 ataaggactc ctctttcttc gctttgccaa gcagatagtg agagcaaagg aagattaacg
     4081 ggaatgtatt accaaaacgt tggggaactc tctccccccg tgggaagcaa cggtgtcgcc
     4141 cttcccatgt ttccctgttg aggtgtgccg gatccggctg cacctcaccg gggcgttccg
     4201 gacgccgaaa cgggcgggca tatctccctt cctcacgagc gacagccgat tctcatcgaa
     4261 ggcagggcag gggaccactg catgaagatc atcgaccagg aaaagatgga acggatcaag
     4321 gctctcctga agacccaccc cagggggctt tccatcacaa acctcgcgtc catcacgggg
     4381 ctgaacagga acctcatcgc caagtacctt gacatgctcg tcatctccgg ccaggtagag
     4441 atacagcagt acgggcctgc caaggtctac ttcctctcgc agaggatccc ggtctccgcc
     4501 atgatcgaga tcacctccga cctcgtcatg gtccttgacc aggaccggaa gatcgttcac
     4561 gtgaacaagc ccttcacccg gttcctcgcc atgaagaggg agaacctcct ggggaagacc
     4621 atcggggagg tggggcatcc cttcctttcc tccatcccgt taccggagtc ctcgtcaccc
     4681 cttgacacag gggacggggg gacgggcgag gtcaggtacg gcagtgcgac gggagacacg
     4741 tttttcagga tcaagagagc cccggcggtc ttcgaggacg gggcggtcgg gctcaccctc
     4801 atcatcgagg acatcaccga gcagaagaag tacgaggaaa cgctccggct ctccgaggca
     4861 aggtaccggg ggatcgtcca ggaccagacc gaattcatca accggtacct gcccgatacc
     4921 accctgacct tcgtgaacca cgccatctgc ctctttgcca atcggcctcc cggggacctc
     4981 ctcggcaagc ggttcatcga tatgatcccc gaggaggaaa ggggacgggt gcgtcaaaac
     5041 atcggggccc tcaccccgga gaaccccacg gccaccatcg agcaccggat ggccgacggg
     5101 cgcgggcggg tgcgctggct gcactggacg aaccgggcca tcttcgacga gaaaggtacc
     5161 atcctggagt accaggggat gggccgcgac atcaccgacc agcgggagac ggccgagcgg
     5221 atctcgcggt acatcctcca gctggagttc atctcgagga aggcggtgga gttcgtccac
     5281 tcggcctccg gcgacgatat ctttctcacc atcgccctgg gggtgaagtc cctctgcccg
     5341 gaggcgggtg tctgcgtcct ctcgagcgag ggagaagggg tcatgaaggt cgcggcggtc
     5401 cccgacgacg agatgaggga ccggattcgg tccatcctgg ggaaggatct cgtcggaacc
     5461 cggatctccc tgtcggcgga tatcatggag aaagtccggg acggcatcct ccaccgggtc
     5521 ccgggcgatc tctacaccca gtgcatcccc cctccctccc ccgatgagca ggtccggctg
     5581 aaggagctgt tcgggaacgt ggacaactac atcatggggc tcacccacgg gaaggacctc
     5641 ttcggggggg tcttcctcat gttccgggaa ggggtcctcc tccagaacga gcacctcctc
     5701 gtgacctacc tgaaccaggc ctcggtggcg gtccagcgcc agatctacca ggaggagctc
     5761 caacggagcc gggaggagta ccggcggatc gtggagacct cggcggaggg gatctgggtc
     5821 ctggatcgaa actaccggtc gacctttctg aatccacgcc tcgcggagat gctggggtac
     5881 gaacccgggg agctcgcggg acgacagatc gaggagttca tgctccccga ggacatcgcc
     5941 gcacatcgcc tgcgtgtcgc cgagcggaga agcggagcaa gcgggcattt cgaacagagg
     6001 ttccggaaga aggacggatc cctgatctgg gtcatggcca atgccacccc cctcttcgag
     6061 tccggtgaat ttaccggcgc ctttgccatg ctcacggatg tcaccaccga gaaacaggcc
     6121 ggggatgccc tgcgggaaag cgaggaacgg tacagggacc tcacggaggc gtccttcgat
     6181 atcatcttca ccatcgaccg ggaagacagg cttgcctatg cgaacaggcg ctgtctggag
     6241 ttcctcggcc gaaggtatga ggaggtggtg ggcaaaccga gggcctccct tttcactccc
     6301 gagataagtg agaaaatgaa ggagaacctc gtgaagatct tctcgaccgg ggagcccctc
     6361 ctcgacagcg cgaatcccgt cgttctcggc gacagggaat tctggctgga cacaaacctc
     6421 atcccgctca agaatgcaga cggttcagtc aatctggtca tggggatttc gcgggacatc
     6481 accctgcgaa agaagactga ggacgccctg cgggagagcg aggagaagta ccgggctctc
     6541 gtggagcagg cccatgacat gatcgtcgtg gtccaggacg ggatcataaa gttctgcaac
     6601 cagaaggttg ccgaaatgtg gggaggcgac atccgggaga tcataggcca gccgtacgaa
     6661 aggttcattg acccggagga gctcccccgg gtccgggaga attacctccg ccggatggca
     6721 ggggaggcag cccccgacct gtatgacacc ctgctcctaa ggcgggacgg gagcagggtc
     6781 cacgtggacc tgagcgcggg gaccatcgtc tttgacggca ggccggccga cctcatcgtt
     6841 ctgcgcgata ttacggagaa gaaagagacc gaagagcagc tgaaatgtac ctacgagctc
     6901 cagaaggcca tcctcgatgc ctcaccggtg ggcatcggtt acgtgtccaa ccgtaccctc
     6961 acctgggcaa ataaggccat gtaccgtatc cagggggtcg agcccctgga gctgcggggg
     7021 cagcatgaaa ggatcgtctt ttccgatgag aaggaatacg agcgggtttg cggacaggtc
     7081 tatcacgaga tcggggagac ggggttcagc gaactggaga ctgccctgaa gcgcaaggat
     7141 ggctccctcg ttcctgtttc cctccgggcg gcaccgatcc ggcccggatc gaccgatctc
     7201 attatcctcg tgaccgacat caccgcccgg aagaaggccg aggaggaact caggaaaacc
     7261 catgcactgc agcaggccat cctcgcggcc tccccgatgg gcatcggggt gaccgcgaac
     7321 cggattctca tctgggccaa cgaggccatg tcccgtatcc acggctatgg gccgggggag
     7381 agggaagggc agtccgcgca attgctctac gaggatgaac gggagtacga gagggtcacc
     7441 cgggagatct ccctcgccat ggaaacaggg gagttccggg tcctcgagac ggtctggagg
     7501 aggaaggacg ggtcccgcgc aaatatccgg ctgaccgtgg gtcccgtccg gccgggctcc
     7561 ccggaggtcc tctctgtggc acgggacatc accgcccaga agaaggcaga ggaggaactc
     7621 gggaagaccc gggcacttaa ggatgccatc ctctcatcct cccccgtggg tatcggcgtg
     7681 tccaggaacc ggttcctcat ctgggaaaac gatgccatgg cttccatctg cggtattgaa
     7741 aaggatgaac tggagggaat gccggtccgg gccgtctacg agagtgatga ggagtatgag
     7801 aggataaccc gggtgggttc cgaggcgata ggaagcggac atttccggga gacggagaca
     7861 gtctgggtga gaaaggatgg ttcatcggtg gatgtccagc tgagcctggg acccgtcgct
     7921 cccggttcct ccgagatcct cgccatcgcc caggacatca ccgcccggaa gaaggccgca
     7981 gagcagctgc ggcagaccca ggccctccag gaggccatcc tcagggcatc acccgtcggc
     8041 attggtctca tatccgaaag aaccctccaa tgggaaaacg atgccatgtg cgaaatcctg
     8101 gggatcaggg aaggggaact gaaagggcag cccctggata ccgtcttcat cgacgagcag
     8161 gagtacggac gaacctcccg ggtcctcgac caggccatca ggcagaacgg ctcaggcgag
     8221 gtggaaacgg tctggaagcg gaaggacgga tcgagggtgg aggtctctct ccgggttgcc
     8281 cctgtacggc cgggctcccc ggaggtcatc ggggtggcga cagatatcac cgatcggaac
     8341 cgggcaggga gggcgctccg cgagagcgag gagaccttcc ggaccatggt caccatcaat
     8401 cccctccccc tctccctcat cgatgcgaaa gggaagtacc tccacgtgaa cccgtccttc
     8461 acccggctct tcgggtacac ccttgaggac atcccggacg ggaaggcctg gttcgccctc
     8521 gcctaccccg atgaagagac gagggaggtg gccatccgtt actggaaggc cgatgccgcg
     8581 gaacacccgg tcggggaggt ccgcccccgg accttcaagg tccggtgcaa ggacgggagt
     8641 ttcaaggaca tcctcttcct ccctgcatcc acccccgccg ggctccaggt ggtggtctac
     8701 caggatctca cccgcgagac catgacggaa aggctgtcgg actccgagga ccggtaccgg
     8761 cacctcgtgg atgacctgaa cgtggggatt taccggagct ccggggatac ggagggacgt
     8821 ttcatctggg gcaacacggg gctcctctcc atcctcggct acgaggatct tgcagacctc
     8881 cggggcgtgc ccgtccggga tatcttccag aagccggggg gaaggaaggc gctcctcagg
     8941 gagctccagg agaagaagtt cgtgaagaac cgcctcctct ccctgaagaa aaaggacggg
     9001 acgccggtga aggtctccgt gaccgccctc gcagagttcg acgagaaggg gaagctccag
     9061 ttcattacgg gcctcgtgca ggaggtcccg cgggattaaa ttccgggcga tatccggggg
     9121 cggtgcgatt taaaaatgag gagctctccc ggtatcccca gaggaaataa tgatatcccg
     9181 gttccaggat gggatgggat atccatctta aatataatac cttggttgca ttgcagacgt
     9241 gctgggcaga tcaatttcac gattgttaac cgagaactat taacataggt gaaaaattga
     9301 ctggtagaga tgtaaaaaga gcgttataaa cgcctccaat cggaaaaaga aacatttatc
     9361 gtcagtattg gggattctcc tacacaccac ctttgaggag ggtgatttga tgcattacgt
     9421 tcagactacc tgcccgtact gcgggaccgg gtgcagtttc aacctggtcg ttgcaggcgg
     9481 aaaagtggcg ggcaccgccc cgtaccaccg ttccccggtg aacgagggta aggtgtgtcc
     9541 caaggggacg tacgcccatg agttcgtcaa cagccccgac aggctgaaaa caccgctcat
     9601 caaaaaggac gggaagttcg tgcccgcctc ctgggacgag gcctatgccc tgatcgtcca
     9661 gaagttcaag gcatacaagc ccgaggagat ggcatgtctc tcctctgcga gggtctccaa
     9721 cgaggagaac tacctgatgc agaagttcgc ccggtcggtc ttaaagaccc cgaacgtgga
     9781 tcactgcgca cggctctgcc acgcctccac ggtcgtgggg ctcgcagggg ccttcggctc
     9841 gggggcgatg acccagtcca tcggcgacat cgcggacgcg aagtgcgtct tcatcatcgg
     9901 gagcaacacc ttcgagcagc acccgctcat cgggcgccgg gtgatgcagg cgaagatgaa
     9961 gggtgcgaag atcatctacg cagacccgcg cctgaccgcg accgggaagc aggcggatct
    10021 gtacctttcc ttctactcgg gcactgatgt ggcagttttc aacggcctca tgcagcacat
    10081 catcaggaac ggctgggaga accgcgagtt catcgaaaag cggacgaagg acttcgacaa
    10141 gctgaagacc gtcgtcatgc aggacaagta cagcctggcc aatgtatcga agatctccgg
    10201 gatcccggag aaggacctcg ccaccacggc ggagtgggtc gcgaagtccg gggcaaccgc
    10261 catcctgtac tccatgggca tcacccagca caccaccggt gtcgataacg tgaagtcggt
    10321 ggcaaacatc cagatgctca cgggcaacct gggcaggccg ggcaccggcg tctgcgccct
    10381 ccggggccag aacaacgtgc agggcgcctg cgacatgggg gcgctcccga acgtctactc
    10441 aggctaccag gcggtcatcg tccccgacaa caagaagaag atgaccgatg cctgggggac
    10501 cgagatcgcc gagggcaagg tcggccttac cgtgaccgat ctgatcaacg tccttgccga
    10561 caagcccggg acggtgaagt gcgcctacat catgggcgag aaccccatgc tctccgaccc
    10621 ggacctccac cacgtggaga agggcctgaa gaacctggag ttcctcgtgg tccaggacat
    10681 cttcatgacc gagaccgcgg agctcgcgaa cgtggttcta cccgcggcct gctatgccga
    10741 gaaggacggg acccagacct ccaccgagcg gcgcgtccag cgctggagaa aggccgtgaa
    10801 cccgccaggc gaggccaggg ccgactggca gatcctcagc gagctcgcgg ggaagatggg
    10861 ctttgcgaag cagttcccct acaagagtgc cgaggagatc tttgccgagg tggcgaaggt
    10921 aaccccgtcc tacggcggca tgtcctatgc ccggctcgag aagcccgagg ccctccacta
    10981 gccctgcccg acggcagacc acccgggaac gcccatcctg cacaaggaga agttcgctca
    11041 ccccgacggt ctcggcgtct tcaccccgct cgagtggaag cccccggccg aggttccgga
    11101 caaggagtac cccctcatca tgaccacggg ccggtgcatc tggcagtggc acacgggcac
    11161 catgacccgc aggtcggtat cccttgagtc cgaggccccg accggctggg tcgaaatgaa
    11221 tccggaggat gcgaaggccc tcgggatcaa ggacaaggag aaggtaaggg ccgtgacccg
    11281 gcgtgggcag atcgagatcg cggccagggt cacgaaggag atcaagaagg gcgaggtctt
    11341 catgcccttc cactacaagg agtgtgcggc caacctgctc acgaacaacg cccttgaccc
    11401 cgtcgccaag atcccggagt acaaggcctg tgcgatacgg atcgagaagc tcccggaggt
    11461 gaagaagtaa tggctctctt tggtggaaaa accgccggtg tgaagaaggg ggacatggtc
    11521 tatgcctgga ccaccgactc cgagctcgcg aagcgtgcgg agtgcggtgg agcggtaacc
    11581 acgttcctca ggcaggccct ggagcagaag ctcgtggacg ccgtgctcgc ggtgaggagg
    11641 ggccaggata tctacgatgc cgtcccgacc ctcatcaccg acccaaagga gctcaccaaa
    11701 accgcggggt cgctccactg cgggacgctg cttctttcca aggtcctcgc gaagcacctg
    11761 aagggtgcga aggaccggaa gatcgcccta accgtcaagg gctgcgacct gatgggcatc
    11821 ctcgagctcg cgaagcggaa cgccatcaac cgggacaacc tctacattgt cggggtgaac
    11881 tgcggtggca cggtcagccc catcaaggcg atgaagatga tccaggaggt gtaccaggtc
    11941 gatccaatga aggtccacaa agaggagatc gacaagggcc agttcatcat cgagtacgag
    12001 gggggccaca agggcatctc catggacgaa ctcgaggaac agggttacgg ccggcgcagc
    12061 aactgccgcc gctgcaagct caaggtcccg cgccaggcag accttgcctg cgggaactgg
    12121 ggtgtcattg gcgagaaggc cggaaaggcc accttcgtcg aggtctgctc cgagaaggga
    12181 gcaaacctcg tgaacggcgc agtcaaggcg ggcatccttg ccaccgaagc ggcaaacccc
    12241 aagggcatcg
//
LOCUS       DATGDI010000021         7023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_2192, whole genome shotgun sequence.
ACCESSION   DATGDI010000021 DATGDI010000000
VERSION     DATGDI010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VKO45_00855"
     CDS             complement(<1..855)
                     /locus_tag="VKO45_00855"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMA04453.1"
                     /translation="MKGLLLSMVPVLVILLLAAQAAPGVQDPTVTYTISLQEDGSAIW
                     NMEYRTPLLTADDLAAFQNYSSQIQSVSLPDYTALMKKSASEASIVTGRPVGVSDFTA
                     DAAVQSGPTGSYGVVHYRCLWTGFASRGESLTVGDIFVGGLFLGKDHALIIRPPQGYT
                     ASLATPTPDSTRDGLTWYGLRSLGAGEPRVVFSRDSFPLTPALLGIGLAVLALGIYAA
                     RRRFRRVPTVPAVEQVPAPVDPLAALELGDRILALLAGRGGSLFQSEIVEILGLPKST
                     VSEAINRLH"
     gene            complement(969..1772)
                     /locus_tag="VKO45_00860"
     CDS             complement(969..1772)
                     /locus_tag="VKO45_00860"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HMA04454.1"
                     /translation="MMARLAEIGIIFLVIAWSMGVAASAAETTLPTTGTSTEIVITDD
                     VQPYVGPLGPDSPLFELKLALENLDEAFTANASEKVMKEMQHAELRISEIKGLLFMNR
                     SVEAERGFQAYSEKMNLTAVELSGIPVRTTGIANAYQKHVKHELVLWDLLQEHPDSAQ
                     LWGAYNSSLALETQFQEKSTIRIEKRLTQLNRITARLVRVENENEPAGKIRGSDTTTP
                     VPTTTDHGKGKEKVGKDQGSAPVTQTTSPSPDNGSGQTKGNGKNGKGSK"
     gene            2237..2734
                     /locus_tag="VKO45_00865"
     CDS             2237..2734
                     /locus_tag="VKO45_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMA04455.1"
                     /translation="MRIGILSDTHDNLPMVDRAVTRIRGISPDLVLHAGDFVSPFVIP
                     RLAALGCPCIGIFGNNDGDRAHLLAKAREAGNVEIRGCFTAGLLGGRSVALLHGHEEG
                     ALGEIAGSGIFEAVIHGHTHRPSVSEKAGTLVLNPGEVCGYLTGKGTVAVLETGPMEA
                     EILEI"
     gene            2724..3272
                     /locus_tag="VKO45_00870"
     CDS             2724..3272
                     /locus_tag="VKO45_00870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA04456.1"
                     /translation="MKSEGRRHRKDPGKTGQEDAGGEEIRPVTGADAPAIAGIFNHYV
                     RRSFAAFPDRELGIHVVRDLLDQAGAFGCYAVEREGEVIGFGLIRPLSPHPNLIMAGE
                     VSYFIRPGLTGKGLGTRLLGVLEGRAGELGMTSLVARVASRNRRSIRFHRRNGFSVCG
                     RIRRVGIKFGRPFDAVILQKLL"
     gene            3452..3907
                     /locus_tag="VKO45_00875"
     CDS             3452..3907
                     /locus_tag="VKO45_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HMA04457.1"
                     /translation="MKPEEYRKIIQNAIAKEVESYVFYRSVTEKVKDAALKSTFKELA
                     GEEIKHREFLEGLLVKPLKELHFDESRDYKISATIQRPDVTIDMKPIDGLKVAIKKEE
                     DAMNLYKGLSAASQDAGMKKMFDSLMKMETSHKARLEDIFTNQAFPEVW"
     gene            complement(3989..4912)
                     /locus_tag="VKO45_00880"
     CDS             complement(3989..4912)
                     /locus_tag="VKO45_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMA04458.1"
                     /translation="MKSLGLVSQTKKDLVVILLLSILVFAVSIAFSLPATLLAFVRAY
                     NSMELDDLIVLSIFLVLALAAFSYRRWQEVKGEIQERRGMEQALLSANRRLQLLNDVT
                     RHDILNSLTGLFLLLGRSEARVAGDPELLSQVQREKEIADLIQRQIAFTRDYQEIGLR
                     KPEWQNVEDVITKAWVGHRIGPVRIDSQIHGLEIFADLLLEKVFSNLIDNAMRYGGPA
                     MTAIRFTSRREGDAVVIVCEDDGLGIPEETRKNLFRRGYGKHTGYGLFLIREILSISG
                     LTITENSRPGTGARFEILVPKGLYRCLPGSS"
     gene            complement(5019..6065)
                     /gene="arsB"
                     /locus_tag="VKO45_00885"
     CDS             complement(5019..6065)
                     /gene="arsB"
                     /locus_tag="VKO45_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HMA04459.1"
                     /translation="MTSESGHNEEVSLGLFEKYLTLWIAICIVIGLLLGRFLPQFGQY
                     LDSLKVGHLSIPIGILLFLMMYPTVIGISFNDVKKATRFPKPMLITIIANWIIAPPLM
                     VLFANIFLRGNPQFIAGVILLGISPCTAMVMWWIKLAKGDLGQGLVNTAINALIMVVL
                     YGVEAAFYLGISSIPVPWDLIALSVILFIAVPVSLAAISRAVLMPRKGEVWFTCRYCT
                     FWGKISIVALLITLVVLFSLEGKIILSEPLLVALIAVPLLLHYVSMIAITFSASWFAH
                     LDYRSAAPSVLIGSSSHFEVAIAVATTLFGLGSGAALATVIGPLMEVPLMLALTKFLW
                     RMRGRFPRKRSEGH"
     gene            complement(6161..6499)
                     /gene="hgcB"
                     /locus_tag="VKO45_00890"
     CDS             complement(6161..6499)
                     /gene="hgcB"
                     /locus_tag="VKO45_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation ferredoxin HgcB"
                     /protein_id="HMA04460.1"
                     /translation="MFDSYRENTLRYYPERCINCKRCTQVCPHAVYAEGDKQTVLARP
                     SECMECGACALNCPTQAIEVQSGVGCAWAMIAAAVRGRDMDSCSCGGPDAPCCGGDGE
                     KETTPCCGDR"
     gene            complement(6499..>7023)
                     /locus_tag="VKO45_00895"
     CDS             complement(6499..>7023)
                     /locus_tag="VKO45_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HMA04461.1"
                     /translation="AYLANRKATPEMRRVEFPLRDRLVLIPVELVMAFKFMVIPVIVI
                     GFLVSLFIAAELVLAVLAGTVLFPILLPWLPTKDFSTKGLILGLLVMIPPAFLGAGTL
                     GPLQLAWAAATLLIFPPFIAYLALNFTGSTTFTSRTGVKKEIFRYIPVMAAMAISGAV
                     LAIIAVAIHLLGVI"
BASE COUNT         1388 a         2085 c         2244 g         1306 t
ORIGIN      
        1 gtggagccgg ttgatggcct cgctcaccgt tgacttgggg aggccgagga tctcgacgat
       61 ctccgactgg aacagggagc cccccctccc ggcgagaagc gcgaggatcc ggtcaccgag
      121 ctcgagggcg gccagcggat ccacgggggc aggcacctgt tcgactgccg gtaccgtcgg
      181 aacacggcgg aacctcctcc ttgcggcata aatcccgagg gcgaggaccg cgaggccgat
      241 gccgaggagg gccggtgtca gggggaacga gtccctgctg aagacgaccc tcggttcacc
      301 cgccccgagg gaccggagcc cgtaccaggt gaggccatcc cttgtactgt cgggcgtggg
      361 ggtcgcgagg gaggccgtat acccctgcgg ggggcggatg atgagggcat ggtccttccc
      421 caggaacagg ccgccgacga agatatcccc gacggtgagg gactctcccc ggctcgcgaa
      481 gcccgtccac aggcaccggt agtggaccac cccgtacgac cccgtcgggc ccgactggac
      541 cgccgcatcg gcggtgaaat ccgagacgcc gaccggcctg cccgtgacga tagaggcctc
      601 ggaagccgat ttcttcatga gggcggtgta gtcgggcagg gagaccgact ggatctggga
      661 cgagtagttc tggaaggcgg cgaggtcatc ggcggtgagg agcggggtgc ggtactccat
      721 gttccatatc gccgacccat cctcctggag gctgatggta taggtgaccg tcgggtcctg
      781 taccccgggt gcggcctggg ccgcgaggag aaggatgacg aggacaggaa ccatggagag
      841 aagaagtccc ttcatcggaa gatcccgtgg ctgcgtacgt gtatcttctt cccccgggaa
      901 cataatcatt ccccgagggg ccttgggctg cgaaaaagag aagaacaggt caggcccggt
      961 ttttgcccct atttcgagcc cttcccgttc tttccattcc ccttggtctg gccggatcca
     1021 ttatcggggg aaggagaggt ggtctgggtg accggtgcgg atccctggtc cttcccgacc
     1081 ttctcctttc ccttcccatg atccgttgtg gttggcacgg gggttgttgt atctgatccc
     1141 cggatcttac cggcaggctc gttctcgttc tctacccgga cgagccttgc cgtgatccgg
     1201 ttcagctgcg tgagtctctt ttcgattcgg atggtggact tctcctggaa ctgggtctcc
     1261 agggcgaggg aactgttgta cgccccccag agttgtgcgc tgtctggatg ctcctggagg
     1321 aggtcccaca ggaccagctc gtgtttgaca tgcttctgat aggcattcgc gataccggtc
     1381 gtgcggaccg ggatcccgga gagttccacg gcggtcaggt tcatcttctc agagtatgcc
     1441 tgaaatcccc tctcggcctc cacggatctg ttcatgaaga gaagcccctt gatctctgag
     1501 atccggagtt cggcatgctg catctccttc atgaccttct cgctcgcatt ggcggtgaag
     1561 gcttcatcga ggttctccag ggcgagcttc agctcgaaca gggggctgtc cgggccgagg
     1621 ggcccgacat agggctggac atcatcggtg atgacaatct ccgttgacgt tcccgtggtc
     1681 gggagggtgg tctccgcggc gctcgccgcc acccccatgg accaggcgat gacaaggaaa
     1741 atgatcccga tctctgccag ccgtgccatc atgggctcac atcgctgccc gtgcccctct
     1801 gggcgatcca gtcccgaatc atggacagat tcagggcgag ccagattccc acgaggatga
     1861 tgatctcgat agtgttgatg gttgccatga ttgagttcac cactcttttc cttgggcccc
     1921 cactttgggc aatccagtct tgaatcggcg tatgattaat ggtgttcacc gttcgggaat
     1981 ccggatatgg tatgccccgt ccccccgggg ttcgggacag ttcctgacgg ttcgaagggc
     2041 caatccgggg catcagggcc gagaaccgga acggccggcg taccggggtc cggacaggag
     2101 gaatacaggc cggtgatgcc ccccgggatt cctcacggac catggcgcgc gggcaggaaa
     2161 aaaaccccgt ctaccggtca tgcgataccg taccaactta tatcccaccc aaggcccagc
     2221 tctcaccggt gaaggaatgc gcatcggtat cctctcggac acccatgaca acctccccat
     2281 ggtggaccgg gcggtgacaa ggatccgggg gatctccccc gacctggtgc tccatgccgg
     2341 tgacttcgtc tcccccttcg tgatcccccg gctcgccgcc ctcgggtgcc cctgcatcgg
     2401 gatcttcggg aacaacgacg gggaccgggc ccacctcctg gcaaaggcga gggaggccgg
     2461 gaacgtggag atccggggct gcttcacggc aggactcctc gggggcaggt ccgtcgccct
     2521 cctccacggc cacgaggagg gtgccctcgg ggagatcgcg gggtccggga tcttcgaggc
     2581 cgtgatccat ggccacaccc accggccctc cgtgagcgag aaggccggga ccctggtcct
     2641 gaacccgggg gaggtctgcg ggtacctgac gggaaagggc accgtggccg ttctggagac
     2701 cgggcccatg gaggccgaga tccttgaaat ctgaagggag gcggcacagg aaggatccgg
     2761 ggaagaccgg gcaggaggac gcggggggcg aagagatccg cccggttacc ggggcggatg
     2821 cccctgccat cgcggggatc ttcaatcact acgtgcggag gagtttcgcc gcctttcccg
     2881 accgggagct cgggattcac gtggtccgcg atctgctgga tcaggcgggg gcctttggct
     2941 gctacgccgt ggaaagggag ggggaggtca tcgggttcgg tctgatccgg cccctctccc
     3001 cccaccccaa cctcattatg gccggggagg tatcgtactt catccgcccc gggctcacgg
     3061 gcaaggggct ggggacccgg ctcctcgggg ttctcgaagg gagggcgggg gagctcggga
     3121 tgacgagcct cgtggcacgg gtcgcctccc ggaacaggag gagcatccgg ttccaccgga
     3181 ggaacgggtt ctcggtctgc gggaggatcc ggagggtcgg gatcaagttc gggaggccct
     3241 tcgacgcggt gatcctccag aagttgctct gagagggata gaccgtatct gcccttccgg
     3301 agctcgcggg agagtacgga tccttgattt ttccctgaca taaaacgccc gttatctgcg
     3361 gatttcgtct attgccgtgc gaataagagg caataaggaa cctatttata gcaggttcaa
     3421 ttaaccaatt acgcgcacgg agttgcgtac catgaagcca gaggagtacc ggaagatcat
     3481 ccagaatgcc atcgcaaagg aggtcgaatc ctatgtcttc taccggagcg tgaccgagaa
     3541 ggtgaaggac gccgccctca agtccacctt caaggagctc gcgggcgagg agatcaaaca
     3601 ccgggagttc ctcgaggggc tcctcgtgaa gcccctgaag gagctccact tcgacgagtc
     3661 ccgggactac aagatctcgg cgaccatcca gcgccccgac gtcaccatcg acatgaagcc
     3721 catcgacggc ctgaaggtcg ccatcaagaa ggaggaggat gccatgaacc tctacaaggg
     3781 gctctcagcc gcctcccagg acgccggcat gaagaagatg tttgactccc tcatgaagat
     3841 ggagacctcc cacaaggccc ggctcgagga tatcttcacg aaccaggcct tcccagaggt
     3901 ctggtaagca catccgggaa cgggccccgg cccgtctccc gttttttcgc gggtcttccc
     3961 tcccccgagc cccggctctt cgcccccctt atgaagaccc gggaaggcac cggtacagcc
     4021 ctttcggcac caggatctca aagcgtgccc cggtgcccgg cctgctgttc tcggtgatgg
     4081 tgaggcccga gatggagagg atctcccgga tcaggaagag gccgtacccc gtgtgcttcc
     4141 cgtagcccct ccggaagagg ttcttccgcg tctcctcggg gatcccgagg ccgtcgtcct
     4201 cgcagacgat gaccaccgcg tccccctccc tccgggaggt gaaccggatg gcggtcatgg
     4261 cagggccccc gtaccgcatg gcattgtcga tgaggttcga gaagaccttc tcgaggagga
     4321 ggtctgcgaa gatctcgagg ccgtggatct ggctgtcgat acggacgggc ccgatccggt
     4381 gcccgaccca ggccttcgta atgacatcct cgacgttctg ccactccggc ttccggaggc
     4441 cgatctcctg gtaatcccgg gtgaaggcga tctgccgctg aatcaggtca gcgatctcct
     4501 tctccctctg gacctgggag aggagctccg ggtcgccggc cacccttgcc tccgaacgac
     4561 cgaggaggag aaacaggccc gtgaggctgt tcaggatatc atgccgggtg acgtcgttca
     4621 ggagctgaag cctcctgttt gccgacagga gggcctgctc catccccctg cgctcctgga
     4681 tctccccctt cacctcctgc cagcggcggt acgagaaggc cgcgagggcg aggacgagga
     4741 agatggagag aacgatgaga tcgtcaagct ccatggagtt gtacgccctg acaaaggcga
     4801 ggagggttgc agggaggctg aaggcgatgg agacggcgaa gaccaggatc gagaggagga
     4861 ggatgaccac gagatccttc ttcgtttgtg agaccagtcc aagggacttc aaaggatttt
     4921 ccatgggaga gctccggaac ttcagatttc cttagtgccg acacggccca tgaagtttcc
     4981 ggtgattctg ccctgatccc tccgcctcag gggtcccgtc agtgtccttc ggatctcttc
     5041 cgcgggaatc gtcctctcat cctccagagg aatttcgtca gggcgagcat caggggaacc
     5101 tccatcaggg gacctatcac cgtggcaagg gccgctccgg accccaggcc gaacagggtc
     5161 gttgcgactg cgatggcaac ctcgaagtgg ctgctggaac cgatgaggac ggaaggcgct
     5221 gcagaccggt aatcaaggtg agcgaaccac gaggcgctga aggtaatcgc gatcatcgag
     5281 acatagtgga gcaggagggg caccgcgatc agtgccacga gaaggggctc ggaaaggatg
     5341 atcttcccct ccagggaaaa caggaccacg agggtgataa gaagggcaac aatcgagatc
     5401 ttgccccaga aggtgcagta cctgcaggtg aaccagactt caccttttct tggcatgagc
     5461 accgcccgtg aaatagctgc gagcgagaca ggaaccgcga tgaacaggat gacgctcaat
     5521 gcgatcagat cccaggggac tggaatgcta ctgatgccca ggtagaacgc agcctccaca
     5581 ccgtagagca caaccatgat gagggcgtta atcgcggtgt tgacaaggcc ctggccgagg
     5641 tctcccttgg caagctttat ccaccacata accatggcgg tgcatggcga gataccgagc
     5701 aggatgactc ctgcgatgaa ctggggattg cccctaagga agatgtttgc gaacagcacc
     5761 atcagtggcg gtgcgatgat ccagttggca atgatggtga taagcatcgg cttcgggaat
     5821 ctggttgctt tcttgacatc gttgaagctg ataccgatca ccgtgggata catcatgagg
     5881 aagaggagga tgccaatggg gatcgataaa tggccaacct tcagggagtc caggtactgg
     5941 ccaaactggg gcaggaatct acccaaaagc aggccgataa cgatgcagat ggcaatccat
     6001 aaagtgaggt atttttcgaa gagtccaagg gatacttctt cattatgacc agattccgat
     6061 gtcatggatc ctcatcacta ttgagaccgg gatcattttc aagggatcgg gatgctgcgc
     6121 cgggcggggg ggcgctgtcc cccctttcag gtggggccat tcaccgatcc ccgcagcagg
     6181 gcgtcgtttc cttctccccg tctcctccac aacagggggc atcgggtccg ccgcagctac
     6241 agctgtccat gtccctgccc ctgacggcgg cggcgatcat cgcccatgcg cagccgaccc
     6301 cgctctgcac ctcgatcgcc tgagtcgggc agttcagggc acatgccccg cattccatac
     6361 actccgacgg ccgggcgagg accgtctgct tatctccttc tgcatagacc gcgtggggac
     6421 aaacctgcgt gcagcgtttg cagttgatgc atcgctcggg gtaatacctg agggtgttct
     6481 ccctgtatga atcgaacatc agatcacccc gagaagatgg atggccaccg cgataatcgc
     6541 gaggaccgct cccgagatcg ccatggcggc catgaccggg atataccgga agatctcctt
     6601 cttcacgccc gttcgggagg tgaaggtcgt tgagccggtg aagttcaggg cgagataggc
     6661 gatgaagggc gggaagatga ggagggttgc ggcagcccac gcgagctgca ggggtccgag
     6721 ggtaccagct cccaggaacg cgggtgggat catgacaagg agccccagta tgagcccttt
     6781 cgtgctgaaa tccttcgtcg gcagccatgg gagaaggatc gggaagagga cggtcccggc
     6841 gagaaccgcc agaacaagct ccgcggcgat gaacaagctc accaggaatc caatgacgat
     6901 gaccggtatg accatgaatt tgaaggccat caccagctct accgggataa gcaccagcct
     6961 gtcgcggagg gggaactcca ccctcctcat ctccggagtt gccttcctgt tcgcgaggta
     7021 cgc
//
LOCUS       DATGDI010000022         5459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_234908, whole genome shotgun sequence.
ACCESSION   DATGDI010000022 DATGDI010000000
VERSION     DATGDI010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234908"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..898)
                     /locus_tag="VKO45_00900"
     CDS             complement(167..898)
                     /locus_tag="VKO45_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HMA04462.1"
                     /translation="MFKPENLVDSAIWQKGLRKENVPGICILDPDGDIVRNLVKTGHA
                     KKSESWPCYHTDMYLFTMDGIDFGIVGYAVGSPFAVLVAEEMFALGCRLLISMTSAGL
                     IDPSVKRPCYMLIEKSIIDEGTSRLYRPWAMVAELDVGLKTRLDGLIHDGTLRSGIAW
                     TTDAPFRETRSRIARMRKIGVTAVEMESSALYSFASAKKKAVICLAHITNEMGTGDNE
                     FDKGGENNTDSSLKLISAMVKRILG"
     gene            complement(1054..1923)
                     /locus_tag="VKO45_00905"
     CDS             complement(1054..1923)
                     /locus_tag="VKO45_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 8 protein"
                     /protein_id="HMA04463.1"
                     /translation="MILTEEEKDSHIIEAVGRSRIVIRDGKVTEVSESRIRECPLAKR
                     FAYPVGEISPGEVKANIEHRMGSFGMCRPDRKVCGRVDFVGFGASELISAGILSGFLD
                     AAVLACDGAGTVVVWNPELVQGVGGRMSGLVQTSPFQEAMDRIEAQGGMILDKGTARI
                     DQAAGVALAIQSGRPRVAVTVALAEDAVRIRECHPDALIIGVHLTATSQEDAERLVSV
                     ADIVTGCASRWIWELGGARACLQAGTSVPVFALTPRGKKLILLKIERMDRPVLVSGGK
                     LPVYGPETPRPLV"
     gene            complement(1923..2600)
                     /gene="pdxT"
                     /locus_tag="VKO45_00910"
     CDS             complement(1923..2600)
                     /gene="pdxT"
                     /locus_tag="VKO45_00910"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HMA04464.1"
                     /translation="MDVRIGVLALQGDVSEHISAFRQALVHRGCGKAPRVIPIRTGGQ
                     IERCDALAIPGGESTTISRLIDRTGMRRAIEEFEGGIFATCAGMVLLSTDAGDPRVKP
                     LGLLDMTVERNAFGRQKESFEADLALEGLRGPAYRGVFIRAPVVKRAGPGVQVISRIP
                     EGIVAVRSGPHLALSFHPELTGDLRIHTLFLNGLLGEPGDNSTPQSTPLPRLWPDREK
                     KVATMKE"
     gene            complement(2590..3486)
                     /gene="pdxS"
                     /locus_tag="VKO45_00915"
     CDS             complement(2590..3486)
                     /gene="pdxS"
                     /locus_tag="VKO45_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HMA04465.1"
                     /translation="MNLNELRHGTELLKRGFASMQKGGVIMDVTDAEQARIAEGAGAV
                     AVMALERVPADIRKVGGVARMADPARIREIMDTVSIPVMAKVRIGHFVEARVLEALEV
                     DMIDESEVLTPADEEFHIEKQGFTIPFVCGARDLGEALRRIHEGAAMIRTKGEAGTGN
                     VVEAVRHMRAIMSGIRRLKGMNQQELTDFARTLEAPASLVIETAALGRLPVVNFSAGG
                     IATPADAALMMQLGADGVFVGSGIFKSETPELTARAIVEAVHHHDEPQVIARVSRSLG
                     AAMPGLDIHTLAPGEVLQTRGR"
     gene            3897..4535
                     /locus_tag="VKO45_00920"
     CDS             3897..4535
                     /locus_tag="VKO45_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04466.1"
                     /translation="MKLISLISGGIDSPVAAYLMIRQGVEIVAVHMDNRPFTDEKNTE
                     KAVKLVKRLEELAGRPIRTYVVPHGPSHIAFARNCRRHLHCLFCRRMMYRVAERIAGK
                     EGADGILTGENLGQVASQTLQNLSVVNQAVKFPIVRPLIGLDKVEIIDIARRIGTYEI
                     STLPGLCCTIVPKKPSTAAKLEEVIEEEENVEIDALVEESLEGMHVLDPEAL"
     gene            4510..4824
                     /locus_tag="VKO45_00925"
     CDS             4510..4824
                     /locus_tag="VKO45_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04467.1"
                     /translation="MYSILKPCKTTAAFEAIPRGHFQLDLALCEKILGKSCTVVANAG
                     VLLIVRDRCEVTIHRDGRLLLKTGSEEVARDQAERIAGWLEGTPAVERRAPGRGSVHR
                     PM"
     gene            complement(4809..>5459)
                     /locus_tag="VKO45_00930"
     CDS             complement(4809..>5459)
                     /locus_tag="VKO45_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HMA04468.1"
                     /translation="DNLQTRFLVNDVCVKHQIPWIYAGAVGTSGMVMPIVPGRTPCLR
                     CLLQSMPGPGVLQTCDTAGVLNTVPAVIASIECTLVYQILTGALDHAQGTSPLILVDG
                     WRHTIDRMTVDRRADCPCCIGGKTDFLDAVSREVITALCGRDAIQILPTARLEISLGE
                     LARRLAPLGEVRSHPLMLTFSSGSTDISIFRDGRAIIRGTRDEAVARSLYARYIGL"
BASE COUNT         1058 a         1752 c         1617 g         1032 t
ORIGIN      
        1 gacaggattc cgtcatattc ttgaaggatg ttcgatttct ccatcgggga tttcacctga
       61 tcggagaatt gaagagtgcg acgcaatgcc ggtggacggc ccccgagacc catcgacatt
      121 atcccccccc gaaggctaaa acgagaatcc ggcaatactt cacatctcag ccaaggattc
      181 gctttaccat ggccgagata agtttgagtg acgagtcggt gttattttct ccgcctttat
      241 cgaattcgtt gtcaccggtc cccatctcgt tggtgatatg ggcgagacag atgaccgcct
      301 tcttctttgc cgatgcaaag gagtaaagcg ccgaggattc catctccacg gcagtcacac
      361 ctatctttct catgcgagca atcctgctcc gggtttcccg gaagggcgca tcggttgtcc
      421 aggcaatgcc gctcctaagc gtcccgtcat ggatcaggcc atcgagcctc gttttcaacc
      481 ccacgtccaa ctcggccacc atggcccagg gacggtaaag gcggctcgtt ccctcgtcga
      541 tgatactttt ctcgatgagc atataacatg gccttttcac cgacgggtcg atcagcccgg
      601 ccgaggtcat cgagatgagg agcctgcagc ccagggcaaa catctcctcg gcaaccagaa
      661 cggcgaaggg agagccgacg gcatagccca cgataccgaa atcgatcccg tccatggtga
      721 acaggtacat atccgtgtga tagcagggcc agctctccga tttcttagca tgtccagttt
      781 tcacgagatt ccgcacgatg tccccatccg ggtcaagaat gcagatgccc ggcacgtttt
      841 cttttctcag gcccttctgc cagatggccg aatcgacgag attctcgggc ttgaacacgg
      901 aagcctcctc gtaatcttcg accccgcagg atccttccgg ccgtttgtcc tctgcgatca
      961 cctcggtcgc ctccgttcag caaccggatt cggaatctcc tctccccgac gaggttcttc
     1021 gtgaaaagtg ccgggatgcc atcggagatt cccctataca agcggccgcg gagtctccgg
     1081 cccgtacacc ggcagtttcc cgccactcac cagaaccggg cggtccatcc gctcgatctt
     1141 caggaggatc agcttcttcc cccggggggt gagggcaaag accgggaccg aggtccctgc
     1201 ctggaggcac gcccgggcgc cgccgagctc ccagatccag cgggaggcgc agcccgtgac
     1261 gatatccgcg acggacacga gccgttctgc atcctcctgg gaggttgcgg tgaggtggac
     1321 gcctatgatc agagcatcgg gatgacactc ccggatccgc acggcatcct ccgcgagggc
     1381 aaccgtgacc gcgacccgcg gtcggccgga ctggatggca agggccactc cagcagcctg
     1441 gtcgatcctc gcggtcccct tatccaggat cataccaccc tgggcctcga tccggtccat
     1501 cgcttcttgg aaaggtgaag tctgaaccag ccccgacatc cttccgccga ccccctggac
     1561 aagctctggg ttccagacga caaccgtgcc cgccccatcg caggcgagca cggctgcatc
     1621 caggaacccg gagaggatgc cggcgcttat cagctccgag gcaccgaacc cgacgaaatc
     1681 cacccttccg cagaccttcc tgtcgggccg gcacatcccg aaggagccca tccggtgttc
     1741 gatattcgcc ttcacttcac ccggggagat ctcccctacc gggtacgcaa acctctttgc
     1801 aagggggcac tcgcgtatcc gggactccga gacctcggtc accttcccat cacggatcac
     1861 gatccgggaa cgaccgaccg cctcaatgat atggctatcc ttctcctctt ctgtcaagat
     1921 catcactctt tcatggttgc taccttcttc tcacgatccg gccagagccg gggaagtgga
     1981 gtggattgcg gggtactgtt atcccccggc tctcccagca ggccgttgag gaacagggtg
     2041 tggatccgca ggtctcccgt gagctccggg tggaaggaga gggccaggtg ggggccggac
     2101 cggaccgcga cgatcccttc ggggattcgg gatatcacct gcaccccggg gccggccctc
     2161 ttcacgaccg gcgcccggat gaagaccccg cggtacgccg gtcccctgag cccctccagg
     2221 gcgaggtccg cctcgaagga ctccttctgc cggccgaaag cgtttctttc cacggtcatg
     2281 tcgaggaggc cgagaggctt cacccgggga tcgcccgcat cggtcgagag gagcaccatc
     2341 cccgcgcagg tggcgaagat cccgccctcg aactcctcga tggcccgccg catgccggtg
     2401 cggtcgatga gccgggagat ggtggtagat tcgccgccgg ggatggcgag tgcatcgcat
     2461 ctctcgatct gcccacccgt ccggatcggg atcaccctgg gtgctttccc gcacccccga
     2521 tggacgaggg cctgcctgaa ggcggagatg tgttcgctca catccccctg gagcgcgagt
     2581 acgccaatcc taacgtccac gagtctgcag cacctccccg ggcgcgagcg tatggatgtc
     2641 cagccccggc atggccgccc ccaggctccg gctcactcga gcaataacct ggggttcatc
     2701 gtgatggtgc accgcctcca cgatcgcccg ggcagtgagt tccggggtct cggacttgaa
     2761 gatgccggag ccaacaaaga ccccgtccgc accgagctgc atcatcaggg ctgcatccgc
     2821 cggcgtggcg attccgccgg cagagaagtt caccaccggg agcctcccga gcgcagccgt
     2881 ctcgatgaca aggctggcag gagcctcgag ggtgcgtgcg aagtctgtca gttcctgctg
     2941 gttcatgccc ttcagccgcc ggatcccact catgatcgcc cgcatgtgtc ggactgcttc
     3001 gacgacattc ccggtgcctg cctctccctt tgtccggatc attgcagcgc cctcgtgaat
     3061 cctccgaagc gcttcaccca ggtcccgggc gccacagacg aagggaatcg tgaacccttg
     3121 cttctcgatg tggaactcct cgtctgcagg ggtcagcacc tcactctcgt cgatcatgtc
     3181 cacctcgagg gcttccagga cccgggcctc aacgaagtgg ccgatccgca ccttcgccat
     3241 taccgggatg gaaacggtgt ccataatctc cctgatacgg gcaggatctg ccatccgggc
     3301 aaccccgcct actttgcgga tatccgcagg taccctctcc agcgccatca cggcgactgc
     3361 acctgcccct tcagcaatac gggcctgttc ggcgtctgtc acatccatga tgacgccccc
     3421 cttctgcatg gaagcaaagc ccctcttcag gagctcggtt ccatgcctga gctcgttcag
     3481 gttcatgggg cacctccgca ggaacccaag ggagggaagg caagctgatc cgggatgaga
     3541 tggttgaacg ccgaccaatc ctgccttgat tcacggaccc ttcgcatatc ccgcacttcg
     3601 tggaaacgag tatctatcgt catgattgtg aagtattcac aattgtgaag tcccattact
     3661 aaaaacttgg catagcggca gggaaggaga gcgccgtcat cacaagcttg atatctcaca
     3721 aaccggaaca gtttgatcaa gcattcacaa ttgtgaactc ccgaccgata aaggcgggga
     3781 gcgggcactg gtcctccgag agagggatga aatggtcccg gatcggatcc tgtgctcttc
     3841 gcggaagcga actccccgca gtccggtctc ctgaaagatg aggatgttgg atctccgtga
     3901 agctcatctc cctcatctcg ggcgggatcg attcccctgt tgctgcgtac ctgatgatcc
     3961 gccagggcgt ggagatcgtc gcggtccaca tggacaaccg ccccttcacg gatgagaaga
     4021 atacggagaa ggccgtgaag ctcgttaagc gcctcgagga gctggcaggc cgccctatcc
     4081 ggacctatgt ggtcccccac gggcccagcc acatcgcctt cgcccggaac tgccgccggc
     4141 acctccactg cctcttctgc cgaaggatga tgtaccgggt cgcggaacgg atcgccggga
     4201 aggagggggc ggacgggatc ctcaccgggg agaacctggg acaggtcgcc tcccagaccc
     4261 tccagaatct gtccgtcgtg aaccaggccg tgaaattccc gatcgtgcgc cccctcatcg
     4321 gtcttgacaa ggtggagatc atcgacattg ctaggcggat cgggacttac gagatctcaa
     4381 cccttcccgg cctctgctgc accatcgtcc ccaaaaaacc atcgacagca gcaaagcttg
     4441 aggaggtcat tgaggaggag gagaacgtcg agatcgatgc ccttgtcgag gagtcactgg
     4501 aggggatgca tgtactcgat cctgaagccc tgtaagacca cggcggcctt cgaggctatc
     4561 ccccgcggtc atttccagct ggacctggcc ctctgcgaga agatcctcgg gaagagctgc
     4621 accgtggtgg ccaatgccgg ggtcctgctc atcgtgaggg accggtgcga agtaaccatc
     4681 caccgggatg gcaggcttct cctcaagact ggttcggagg aggtcgcacg ggaccaggcg
     4741 gaaagaatcg ccgggtggct ggaggggact ccggcggtgg aaaggcgggc accgggcagg
     4801 ggaagtgttc acaggccgat gtagcgtgca tagagggacc gggccacggc ctcgtccctc
     4861 gtcccgcgga tgatggccct gccgtcgcgg aatatcgaga tatccgttga tcctgaactg
     4921 aaggtgagca taagtgggtg ggaccggacc tcgccgagtg gcgccagccg ccttgcgagc
     4981 tccccgaggg agatttccag ccgggctgtc gggaggatct ggatggcatc cctcccgcag
     5041 agggcggtga tcacctcgcg ggagacggca tccaggaaat cggtcttccc cccgatgcag
     5101 caggggcagt ctgcccggcg atcgacggtc atccggtcga tggtgtggcg ccacccgtcg
     5161 acgagtatca ggggagaggt tccctgagca tgatcaagcg ccccggtgag gatctggtac
     5221 acgagggtac attcgatgga agcgatgacg gcgggaaccg tgttcaggac ccctgccgtg
     5281 tcgcatgtct gaagcacccc cggaccgggc atggactgga ggaggcacct gaggcacggt
     5341 gtccggcccg gaacgatggg catcaccata cccgaggttc ccaccgcgcc ggcatagatc
     5401 caggggatct ggtgtttcac gcagacatca ttcaccagga accgggtctg gaggttgtc
//
LOCUS       DATGDI010000023        12766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_293078, whole genome shotgun sequence.
ACCESSION   DATGDI010000023 DATGDI010000000
VERSION     DATGDI010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 12766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12766
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293078"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VKO45_00935"
     CDS             <1..444
                     /locus_tag="VKO45_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HMA04469.1"
                     /translation="TDLLDPRAGDRVLEIGAGSGYQAAILSELASEVISVERIPSVAA
                     QARRNLSVAGCRRVEVVGGDGTLGYSQRAPYQGILVTAGTPEVPPPLIGQLADGGRLV
                     APVGPHGIQELVRIVHEGEKIRRESHGGVVFVPLIGEYGWKEDAG"
     gene            434..1054
                     /locus_tag="VKO45_00940"
     CDS             434..1054
                     /locus_tag="VKO45_00940"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HMA04470.1"
                     /translation="MPDNLILDITRPLGLEVPLYPGSERPIFRRRELDGFTVTDLQLT
                     THTGTHLDAPSHTLDGGTTVDRIPLERLVGKVTVLDLQDTGDAITAEDLPGRLPKGRG
                     VLFRTRASGARSFGTEFPHLTPEAAILLAGAAPRVVGIDSPSIEAFGEGMVHREFLER
                     GIPILELLDLSAAPAGEYLMVALPLRLEGLDGSPVRVILIREGGSA"
     gene            1051..1653
                     /locus_tag="VKO45_00945"
     CDS             1051..1653
                     /locus_tag="VKO45_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04471.1"
                     /translation="MTLLLEAVMLLEDTVQGSGCEDGIATVRRRPDAPRCQYERGASL
                     EASFGGRTTHIVTGSPVQTETRLSSLFGRDLAAPEQRTAALGIVNAVTGFLCLARKLH
                     ACDPASYEACLKELGEEIGGRRIYTPVDIPVLSRDLAPLAAGNPGDAEILVLTGETLV
                     QETGDGLRDRSPKDGMLLLGPSTEGVASLLNLPHWCPYGR"
     gene            1790..3040
                     /locus_tag="VKO45_00950"
     CDS             1790..3040
                     /locus_tag="VKO45_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase/phosphoglucosamine mutase"
                     /protein_id="HMA04472.1"
                     /translation="MLFGSSGIRRRYDLSLAELALSLGSALAATAEEVAVGMDTRTTS
                     PVLTEAAIAGLLSGGARVTFGGIAPTPTVAFAARTRDAGLMITASHNPEEYNGLKVFN
                     PDGSSLAPPQQEEIEGRMGALQRKNWECQGDLEEADLVTGHMSAILDRREVSGDLSVV
                     IDCGNGAGSVITPELLSRMGVATLPLNCHTGGHFARPSEPAEAGLGYVAEMVRAGSAD
                     CGVVHDGDADRMMAFDGRGRFIDGDRLLVLFARYLGAKRVVTTSDASMVVDDIAEVER
                     TSVGDTYVSARLREWGDFGGEPSGAWIFPGHSLCPDGIYAAALLCEMASEGDLAKELD
                     GIPRYPILRESMPCADSGEVLRRLGAESPTDGIRREEEDGWFLIRASGTEPKVRITTE
                     GKTRAATKKLAGKARAMLSRAESA"
     gene            3063..4262
                     /gene="glmU"
                     /locus_tag="VKO45_00955"
     CDS             3063..4262
                     /gene="glmU"
                     /locus_tag="VKO45_00955"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sugar-1-phosphate
                     nucleotidylyltransferase/acetyltransferase"
                     /protein_id="HMA04473.1"
                     /translation="MECVVLAAGEGRRMRPLTANRPKVMLPLANRPILEHLIVAARDA
                     GITEFILVVGYREREVRSCFGNGERLGVRIRYATQRRQLGTGDALRACEGLVTGPFLL
                     LNGDLVTRSGDLRALVGHEPPVMGVSRSARPQDFGTVRMEGDRVLDLAEKVPNPRGDL
                     VNAGAYLLDPEIFGLLGSIGPSERGEYEITDALSALIREEKLAGYPLSFWLDVGYPWD
                     LLSANEMLLSDRDFGREGTVEPGAVLDGDVAVGPGSVIRTGTVIEGPCIIGRDCRIGP
                     HAYIRGATSVGNDCHIGHGSEVKNSVILAGTKVPHLNYVGDSIIGSSCNLGAGTKIAN
                     LRNDRKPVQACGRDTGRLKFGAVVGDGVSFGINCSLNAGTVVGSGCSFAPQSYIHGCI
                     MDGTEMR"
     gene            4267..5418
                     /locus_tag="VKO45_00960"
     CDS             4267..5418
                     /locus_tag="VKO45_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMA04474.1"
                     /translation="MQAVILAAGEGTRLRPLTGGKPKAMIPVANRPIIEHVIDALLAN
                     GIRDIVVVVGYRREYVQRFLNGLDIPVTVVVQEKQLGTAHALRAAAPEISDDFLVLPG
                     DNYIDADSIAAIRGEHCAMLVKDHPSPSNFGVVVVKNGCIADIQEKPAEAATQTVSTG
                     ILSLPRETLRSLTKNEMPDAVMGLIRKGMRMKAVMAGDWQDAIYPWDLLRMNRSLLSH
                     ITPRKAGKMSGNITFSGIVSVGTGTALGPNSTITGPVVIGEDCEIGPGTCILPGTSIG
                     SRVTIEPFTVIGNSLVMDDTFVGSHSRIRDAVIGEGCRIADHATTLPAPSHLPIEGKV
                     VKAEFGAVLGDRVTAAPFTVFRSCIVGNGVEIREGRTVTQILPDNTLVM"
     gene            5420..7159
                     /gene="glmS"
                     /locus_tag="VKO45_00965"
     CDS             5420..7159
                     /gene="glmS"
                     /locus_tag="VKO45_00965"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HMA04475.1"
                     /translation="MCGIVGYAGRRRAAPLIIDGLRRLEYRGYDSFGVATLGSHLDLY
                     KRCGKIPASLPDTRAMKGTLGIGHTRWATHGEPSDVNAHPHTDCTGRIAVVHNGIIEN
                     YASLRKRLEKKGHRFRSETDTEVIAHLIEEKYEGDLLAAVEATVGSLEGSYAILAVAE
                     GDRRIVAARCASPLVLGLGDGETLAASDMTPLLEYTERVVFPEDGDVISITPQGAEIR
                     HGGEAIERPVELVNWCADDVRKGGFEHYMLKEIYEEPRVFYETARTADPTPLVDLLRE
                     TPALTIVACGTSYHAGLIAKYLLEECAGIPVRVEQASEFRYAVPPSRNPVLAVTQSGE
                     TADTLWAVRAAKSRNCPAVALTNVVGSSVTRLADSAIYTRAGPEVGVAATKSFISQLA
                     VFLSVADRLGGGRLAGSIASAHLAIGDTLQADVGEAVTLCREARSLFFIGRGPFYPVA
                     LEGALKMKEISYIHAEGYAAGELKHGPFALLTPETPAIAICLPGPTYRAMISNIKEIK
                     ARGAPVIGIGLPGDRDLGDIVDVFLPVTGEGAWVQVLTTSVLLQLLAYHTAKSLDTDI
                     DRPRNLAKSVTVE"
     gene            7156..7743
                     /locus_tag="VKO45_00970"
     CDS             7156..7743
                     /locus_tag="VKO45_00970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMA04476.1"
                     /translation="MIEYGRNTLGPNARIFEPVTLGFPSRDRIGCESFRGTVIGRNAF
                     LCPGTTMYCDVEIGDDFTCGHNVLIREGTRIGDRVAIGTGTVIEGTTEIGDDVRIQSL
                     VFIPVNTTIGNGVFIGPHAVLTNDRYPPFGGPELKGPVLSDRSIIGANATILSGIVIG
                     EGACVAAGAVVTSDVPSCKLAIGSPARIRDLPEED"
     gene            complement(7810..9144)
                     /locus_tag="VKO45_00975"
     CDS             complement(7810..9144)
                     /locus_tag="VKO45_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HMA04477.1"
                     /translation="MPGKRLAERFNGLISAGDLRIGVIGLGYVGLPLALSFSKKFPVV
                     GYDVSREKVGMINRGETPIVYIDPGEIRAATARSFTASTDPGSLAVCDAILICVPTPL
                     TSEREPDLSYVKSACMTIAGILRPGMFVILESTTYPGTTEEVVIPILEGTGLKAGSDF
                     GVAYSPERIDPGNTKFRLEEIPKVVGGITPECTGIAASIYETVIPEVIRVRDPRSAEA
                     TKMVENIFRNVNIALVNELALIFEHMGIDTWEVIRAASSKPYGFMTFYPGPGVGGHCI
                     PLDPYYMAYKAKRCGFIPRFIETSGEINDFMRIHVVNLVDRGLREANRTVAGARIALV
                     GLAYKKNIDDVRESPSLRILEELITLGAEVRVFDPFVEAVTVQGREFRSSGSLEDVLS
                     GSDCAVFAVDHDGFRERKIEDLARLMRTPVVIDCRDMFERVPGIIYHGIGKP"
     gene            9237..10358
                     /locus_tag="VKO45_00980"
     CDS             9237..10358
                     /locus_tag="VKO45_00980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMA04478.1"
                     /translation="MIMLVYLGIALFFFSLLPHIIYLVGTSLEKPPDPREPPGDLPPI
                     TLILSAYNEEQILPARVRNLMESTYPKEKVEIILIDDFSGDQTARVAAESLGGSGFRH
                     RVIRNPERLGTNRSYNRAIPMASHPIIVTTDADVFFEPDALVRVVTRLVSDDDIVAVC
                     GDLRPFPSGTSTTAFESVYRNFFGRMCEWESRIDSTYTFNGALVAFRKDRVNRIEDKK
                     GSDDANTAFEAIRRGYRAVYEIRAVVYEYIPPDFSAQYRQKTRRAKRLIEATLANLDL
                     LGHDRPFSRYFYPLRIFMYVVSPALFFAALFLFGAGVLLLSPALFLILVAVFALVTVA
                     WRRNFAVALVLNQFYLLVGLLRLPTETRIWESTTKRITG"
     gene            complement(10348..10473)
                     /locus_tag="VKO45_00985"
     CDS             complement(10348..10473)
                     /locus_tag="VKO45_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HMA04479.1"
                     /translation="MLNTSFNLHGEPMVFHPADALHTWLSSGVDSLIMESFVITR"
     gene            complement(10517..10813)
                     /locus_tag="VKO45_00990"
     CDS             complement(10517..10813)
                     /locus_tag="VKO45_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04480.1"
                     /translation="MRWPEDFFWEEWLFIGGVVLVLALLFIAGIVTRGWVTITGASIL
                     LAGGAVALAVALSRTRLPAYNDPGMEELVFLISVLGVFGLVFGAGVAVGTWLYQ"
     gene            complement(10800..12641)
                     /gene="asnB"
                     /locus_tag="VKO45_00995"
     CDS             complement(10800..12641)
                     /gene="asnB"
                     /locus_tag="VKO45_00995"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HMA04481.1"
                     /translation="MCGIAGIFNQNNGTVDKSLLEAMSDRIRHRGPDGGGIHLDGSIG
                     LAHRRLAIIDLTDAAEQPMTNEDGALWLVYNGEIYNYLELREELAGRGHTFRSQGDSE
                     VVLHAYEEWGKDCLARFNGMWAFALWDSRRSELFCARDRFGIKPFYYAIVGDTLLFAS
                     EIKALLAHPGVGKKPDDRLLSTFLAWGVLDHTGGTMFEGIHQLQPAHRITFGRAGGVP
                     EKYWDLTMNPEPGRPGPIQDEEAAHAFQGTLTDAIRIHLRSDVPVGTCLSGGVDSSTI
                     AIIINRLIRAEHPRSIGNWQKTFSIIFSDIRFSEERFIDEVVAASGVDSRKAAIGPRE
                     ILEDLQRLLWMQDEPFGSLSIAAQYQVMKLASKSVKVVLDGQGADEQLGGYLAYQGSY
                     IVGLVRGLHLLTALRELVGGLRIHGPFFLGAVAQILIRKQRRSTLRGEIPVVSRYGGS
                     LDEALKREILETNLTGLLHWEDRNSMAFSIEARVPFLDQQVAEFLAVLPLSQKIRKGV
                     TKYVLRKAIKGLVPEPVRCRMDKMGFVTPEEVWMKNELHQFVSEVLSSAGFRARKYWD
                     ADRVIASYTEFVKGGSPYSMEFWRIICTELWLRMMFDARGESKDALA"
BASE COUNT         2247 a         4234 c         4023 g         2262 t
ORIGIN      
        1 accgacctcc ttgacccccg cgccggggac cgggtcctgg agatcggtgc cggcagcggg
       61 taccaggcgg cgatcctctc ggagctcgcc tccgaggtta tctccgtcga acgcatcccc
      121 tccgtggccg cccaggcgag gcggaacctc tcggtggcgg ggtgccgccg ggtggaggta
      181 gtgggagggg acggtaccct cgggtattcc cagagggccc cctaccaggg gatcctcgtc
      241 accgcaggta ctccggaggt ccccccgccc ctcatcgggc agctcgccga cgggggccgg
      301 ctcgttgcac ccgtcggccc gcacgggatc caggagctgg tgcggatcgt ccacgaggga
      361 gagaagatcc ggagggagtc ccacgggggg gtcgtcttcg tgcccctcat cggggagtac
      421 gggtggaaag aggatgccgg ataacctcat cctcgatatc acccgtcccc tcggcctcga
      481 ggttcccctt taccccggga gcgaaagacc catcttccgg aggagggagc tggacggatt
      541 tactgtcacc gatctccagc tcaccactca cacggggacc cacctggatg caccctccca
      601 tacccttgat ggggggacga ccgtggaccg gattcccctc gagaggcttg tgggaaaggt
      661 gaccgtcctg gacctgcagg atacggggga tgccatcacc gcggaggacc ttccgggtag
      721 gcttccaaag gggagaggag tcctcttcag gacccgggcc tccggagcca ggtccttcgg
      781 aaccgagttt ccccacctca cccctgaggc ggccatcctc cttgccgggg cagccccccg
      841 ggtagtggga atcgattccc cttccatcga ggcgttcggg gaaggcatgg tgcaccggga
      901 attcctggaa agggggatcc ccatcctcga gctcctcgac ctctccgccg cccctgccgg
      961 tgagtacctc atggtggccc tccccctcag gctcgagggt ctggacggat ccccggtccg
     1021 ggtgatcctc atcagagagg ggggatcggc atgacccttc tcctcgaggc agtgatgctt
     1081 cttgaggaca ccgtccaggg gagcggctgc gaggacggga ttgccaccgt ccggcgcagg
     1141 cccgatgccc cccggtgcca gtacgagagg ggtgcctccc tcgaggcatc cttcggcggg
     1201 cggaccaccc acatcgtgac gggttccccg gtccagaccg agacccggct ctcctccctc
     1261 ttcggccggg acctcgcggc gccggagcag cggaccgccg ccctcggtat cgtgaatgcc
     1321 gtcacgggct tcctctgcct cgcccggaag ctccatgcct gcgaccccgc ctcctatgag
     1381 gcgtgcctga aggagctcgg ggaggagatc ggggggagga ggatctacac gccggtcgat
     1441 attccggtcc tctcccggga tctggcaccc ctcgcagccg ggaatccggg cgatgcggag
     1501 attctggtgc tcaccgggga gacgctcgtc caggaaacgg gggatgggct gagggacagg
     1561 tccccgaaag acgggatgct cctcctcggg ccgtccaccg agggggtggc ctccctcctc
     1621 aatctgcccc actggtgtcc gtacggccgc tgatccagtc cggcccacgg gcgctgatcc
     1681 aattcgcccc cgttgccact gatcccaagg gccccaaagc cattgatcca attccggtcc
     1741 cgtgacgggt gctgaaaaac ctataacaca taaggggaaa tggtacctga tgctcttcgg
     1801 ctcatcgggg atcaggcggc gctatgacct ctctctcgcg gaactcgcgc tgtccctcgg
     1861 ctcggccctc gccgcaaccg cggaggaggt ggcggtgggg atggacaccc gtaccacgag
     1921 cccggtcctc acggaggccg ccatcgcggg gctcctttcg gggggagccc gggtcacctt
     1981 cgggggaatt gctcccaccc ccaccgtcgc ctttgccgcc cggacccggg atgcgggact
     2041 catgatcacc gcctcccaca accccgagga gtacaacggc ctgaaggtct tcaaccccga
     2101 tggttcgtcc ctcgctcccc cgcagcagga ggaaatcgag ggaaggatgg gggctctcca
     2161 gaggaaaaac tgggagtgcc agggtgacct ggaggaggcc gacctcgtca ccgggcacat
     2221 gagcgccatc ctggaccgga gggaggtctc cggtgatctg agcgtggtga tcgactgcgg
     2281 gaacggggcg ggttccgtca ttaccccgga actcctctcc cgcatgggag tcgccaccct
     2341 ccccctcaac tgccatacgg ggggacactt cgcccggccc tctgaaccgg ccgaggcggg
     2401 gctcgggtac gtggcggaga tggtgagggc gggatcggcg gattgcgggg tggtccatga
     2461 cggggacgcc gaccggatga tggccttcga cgggaggggg aggttcatcg acggtgaccg
     2521 gctcctcgtc ctcttcgccc gctaccttgg tgcgaagcgg gtcgtcacca cctccgatgc
     2581 ctccatggtc gtcgatgaca tcgccgaggt agagcgaact tctgtcggcg acacgtacgt
     2641 ctcggcccgg ctccgggaat ggggggactt cgggggcgag ccgtcgggcg cgtggatctt
     2701 cccgggccac tccctctgcc ccgacgggat ctacgcggcc gccctcctct gcgagatggc
     2761 ctccgagggg gacctcgcga aggagctcga cgggattccc cggtacccca tcctccggga
     2821 gtccatgccg tgcgccgact ccggggaagt gctccggcgc ctcggggcgg agtcccccac
     2881 ggacgggatc cgccgggagg aggaggacgg gtggttcctc atccgggcga gcgggaccga
     2941 gccaaaggtc cggatcacca cggaggggaa gaccagggcg gccacgaaga agctggccgg
     3001 aaaggcccgg gcgatgctct ctcgggcgga aagtgcttaa accggcgggg cgagtacttt
     3061 tcatggaatg cgtggtgctc gcggccggtg aaggccggcg catgcgcccc ctgacagcga
     3121 accggccgaa ggtgatgctt cccctcgcaa accggcccat tctcgagcac ctcatcgtcg
     3181 ccgcccggga tgcggggatc acggagttca tcctcgtggt cgggtaccgg gagagggagg
     3241 tccgatcctg cttcgggaac ggggagcgcc tgggggtcag gatccggtat gccacccagc
     3301 ggcgccagct cgggaccggc gatgccctgc gggcatgcga ggggctggtc accggcccct
     3361 tcctcctcct gaacggcgac ctggtgaccc ggtcaggcga tctccgcgcc ctggtcggcc
     3421 acgaaccgcc ggtcatgggc gtctcccgct ccgcccgtcc gcaggacttc gggaccgtga
     3481 ggatggaggg cgaccgggtc ctcgatcttg ccgagaaggt cccgaacccc cggggggacc
     3541 tggtgaatgc aggggcctat cttctcgacc cggagatctt cgggctcctc ggatccatcg
     3601 gcccgtcgga acggggggag tacgagatca ccgacgccct ctcggccctc atccgcgagg
     3661 agaaactcgc gggatatccc ctctccttct ggctcgacgt gggctacccc tgggacctcc
     3721 tctccgcgaa cgagatgctc ctctccgacc gggacttcgg gagggaggga accgtggagc
     3781 ccggcgcggt gctcgacggg gacgtggcgg tggggcccgg gtcggtcatc cggaccggca
     3841 ccgtcatcga gggtccctgc atcatcggga gggactgccg gatcggcccc cacgcctata
     3901 tccggggggc gacctccgtc gggaacgact gccacatcgg gcacgggtcc gaggtgaaga
     3961 actccgtcat cctcgccggg acgaaggttc cccacctcaa ctacgtgggg gacagcatca
     4021 tcggctcttc ctgtaacctc ggggcgggga cgaagatcgc gaacctcagg aacgaccgga
     4081 aaccggtgca ggcctgcggc cgggacacgg gcaggctgaa gttcggtgcc gtcgtcgggg
     4141 acggggtctc cttcggcatc aactgctccc tgaacgccgg gacggtcgtg gggagcggct
     4201 gcagcttcgc cccccagtcg tacatccacg gctgcatcat ggacggaacg gagatgaggt
     4261 gaacccatgc aggcggtgat actcgcggcg ggcgagggga cgaggctccg gcccctcacc
     4321 ggcggcaaac ccaaggcgat gataccggtg gcgaaccggc ccatcatcga gcatgtcatc
     4381 gatgccctcc tcgcaaacgg catccgggac atcgttgtcg tggtcggcta ccggcgggag
     4441 tacgtccagc ggttcctgaa cggcctcgac atcccggtca cggtggtggt gcaggagaag
     4501 cagctcggga ccgcccacgc cctccgggcc gccgcccccg agatcagcga cgatttcctg
     4561 gtcctccccg gggacaatta catcgatgcc gactccatcg cggcgatccg gggcgagcac
     4621 tgtgccatgc tcgtgaagga ccacccgagc ccctccaact tcggggtggt cgtcgtgaag
     4681 aacgggtgta tcgccgacat ccaggagaaa ccggcagagg cggctaccca gacggtgagc
     4741 acgggaatcc tctccctccc ccgggagacc ctccgctccc ttacgaagaa cgagatgccc
     4801 gacgcggtca tggggctcat ccggaagggg atgaggatga aggcggtcat ggccggcgac
     4861 tggcaggatg ccatctaccc ctgggacctg ctccggatga accggagcct cctctcgcac
     4921 atcaccccga gaaaggcggg gaaaatgtcg gggaacatca ccttttccgg catcgtttcg
     4981 gtgggcaccg ggacggccct cgggccgaat tccaccatca cgggtccggt cgtcatcggg
     5041 gaggattgcg agatcgggcc ggggacctgc atcctccccg gcacctccat cggttcccgg
     5101 gtcaccatcg agcccttcac ggtcatcggc aattccctcg tcatggatga caccttcgtc
     5161 gggtcccact cccggatccg ggacgcggtc atcggggagg ggtgcaggat cgcggatcat
     5221 gcaaccaccc tccctgcccc ttcccatctc cccatcgagg ggaaggtggt gaaggcggag
     5281 ttcggggccg tcctcggtga ccgggtgacg gccgccccct tcacggtctt ccggagctgc
     5341 atcgtcggga acggggtgga gatccgggaa gggcggacgg ttacccagat cctcccggac
     5401 aacaccctgg tgatgtgaga tgtgcgggat cgtggggtac gcggggcggc ggagggccgc
     5461 ccccctcatc atcgacgggc tccggcgcct cgagtaccgg ggctatgact ccttcggggt
     5521 cgcgaccctg ggatcccacc tggatctcta caagcggtgc gggaagatcc cggcgagcct
     5581 cccggacacc cgggcgatga agggcaccct cggcatcggg cacacccggt gggccaccca
     5641 cggggagccg agcgatgtga acgcccatcc ccacaccgac tgcacgggcc ggatcgcggt
     5701 ggtccacaac ggaatcatcg agaattatgc atccctccgg aagcgcctcg agaagaaagg
     5761 gcaccggttc cggtcagaga cggacaccga ggtcatcgcc cacctcatcg aggagaagta
     5821 tgaaggggac ctcctcgccg cggtggaggc aacggtcggg tccctcgagg gctcgtacgc
     5881 catcctcgcg gtcgccgagg gggacaggcg tatcgtggcc gcccggtgcg cgagccccct
     5941 cgtcctcggc ctcggggacg gggagaccct cgccgcctcg gacatgaccc cgctcctcga
     6001 gtacaccgag cgggtcgtct tccccgagga cggggacgtc atctccatca caccccaggg
     6061 ggccgagatc cggcacggag gcgaggccat cgagcggccg gtcgagctcg tgaactggtg
     6121 cgcagatgac gtgagaaagg gcggctttga gcactacatg ctgaaggaga tctatgaaga
     6181 gccccgggtc ttctacgaga cggcccggac cgcggatccc actcccctcg tggatctcct
     6241 ccgggaaacc cccgccctca ccatcgtggc ctgtggcacc tcctaccacg cggggctcat
     6301 cgcgaagtac ctcctcgagg agtgcgccgg gattccagtc cgggtggagc aggcatcgga
     6361 gttcaggtac gccgtcccgc cgtcccggaa cccggtcctc gccgtgaccc agtccgggga
     6421 gaccgccgac accctctggg ccgtccgggc cgcgaagtca cggaactgcc cggccgtcgc
     6481 cctcacgaac gtcgtcgggt cctcggtgac ccggctcgcg gactcggcca tctacacccg
     6541 ggcggggccc gaggtcgggg tggccgcaac caagtccttc atctcccagc tcgcggtctt
     6601 cctttcggtg gcagaccgtc tcgggggagg caggctcgcg gggtccatcg cctcggccca
     6661 cctcgccatc ggcgataccc tccaggcgga cgtgggggag gccgtcaccc tctgccggga
     6721 ggcacgctcc ctgttcttca tcggccgggg tcccttctac ccggtcgccc tggagggcgc
     6781 cctgaagatg aaggagatct cctacatcca tgccgagggg tacgccgccg gtgagctgaa
     6841 gcacggcccc tttgccctcc tcacgccgga aacccctgcc atcgccatct gcctcccggg
     6901 acccacctac cgggccatga tctcgaacat caaggagatc aaggcaaggg gggcgccggt
     6961 cataggcatc ggccttcccg gtgaccggga tctcggggac atcgtcgacg tcttccttcc
     7021 ggtcacgggg gagggggcct gggtccaggt cctcacaacc tcggtcctcc tccagctcct
     7081 ggcctaccac accgcaaaga gcctggacac cgacatcgac cgcccccgga acctcgcaaa
     7141 gagcgtgacc gtcgaatgat cgagtacggc aggaacaccc tcgggcccaa cgccaggatc
     7201 tttgagcccg tgaccctcgg gttcccttcc agggaccgga tcggctgcga gagcttcagg
     7261 ggcacggtga tcgggaggaa tgccttcctc tgcccgggga ccaccatgta ttgcgacgtc
     7321 gagatcggcg acgatttcac ctgcggccac aacgtgctga tccgggaagg gacccggatc
     7381 ggggacaggg ttgcgatagg taccgggaca gtaatcgagg gaaccaccga aatcggagac
     7441 gatgtccgga tccagtccct cgtcttcatc ccggtgaaca ccaccatcgg aaacggtgtc
     7501 ttcatcggcc cccacgcggt cctcacgaac gaccggtacc ctccttttgg cgggcccgag
     7561 ctcaaaggcc cggtcctctc ggaccggtcg atcatcgggg cgaacgcgac gatcctttcc
     7621 ggcatcgtca tcggggaagg ggcatgcgtt gcggcggggg cggtcgtcac ctccgatgtc
     7681 ccctcctgca agctcgccat cggttccccg gcccggatcc gggacctccc ggaagaggac
     7741 tgaggaggct ccctgcggag ctgattcatt cgccccggct gacagggtcc ggatgggggt
     7801 cgtccccgtt cagggctttc cgatcccgtg atagatgata ccgggcaccc tttcgaacat
     7861 gtcccggcag tcgatgacaa cgggggttct catgagccgg gccagatcct cgatcttcct
     7921 ttccctgaac ccgtcgtgat ccaccgcgaa aaccgcgcaa tccgacccgg agaggacgtc
     7981 ctcgagggat cctgaggaac ggaactccct gccctgaacc gtgacagcct cgacaaaggg
     8041 gtcaaacacc ctcacctccg cccccagggt gatcagctcc tccaggatcc ggagggaggg
     8101 agattccctc acgtcatcga tgttcttctt gtaggcaagg ccgacgaggg cgatcctggc
     8161 ccccgccacc gtccggttcg cctcgcggag accgcggtcc acgaggttta ccacgtggat
     8221 ccgcatgaag tcatttattt ctcccgacgt ctcgatgaac cgggggataa acccgcaccg
     8281 ctttgccttg taggccatgt agtacgggtc gagaggaata cagtggcccc cgaccccggg
     8341 acccgggtaa aaggtcatga atccgtaggg cttcgaggag gccgcacgga tcacctccca
     8401 ggtatcgatc cccatgtgct cgaagatgag ggccagttca ttcacgaggg cgatgttcac
     8461 gttccggaag atgttttcca ccatcttcgt tgcctcggcc gacctgggat cgcgtacccg
     8521 gataacctcc gggatgaccg tctcgtagat ggaggcggca attccggtgc attccggggt
     8581 gataccgccg accaccttgg gaatttcctc cagccggaac ttcgtgttcc ccggatcgat
     8641 ccgctccggg gaataggcaa ccccgaagtc ggatcccgct ttcaggccgg ttccctcgag
     8701 gatcgggatg accacctcct cggtggttcc cgggtaggtg gtgctctcga ggatcacaaa
     8761 catcccggga cggaggatgc ccgcgatggt catacaggcg ctcttcacat agctcaggtc
     8821 aggctcccgt tcgctcgtca gaggtgtcgg gacgcagatg aggatcgcgt cgcagaccgc
     8881 tagacttccc ggatcggtgg aggccgtgaa ggatctggcg gtggcagccc tgatctctcc
     8941 ggggtcaatg tacacgatgg gagtttcacc ccggttgatc atcccgacct tctcccggct
     9001 gacgtcgtac ccgaccaccg ggaacttctt cgagaagctc agggcgagag gaagaccgac
     9061 atatccgagg ccgatgaccc cgatccggag gtcccccgca ctgatcagcc cgttaaacct
     9121 ttccgcaagt cgttttccag gcatcccgtt catttacagc ttgccatggg ggatcgataa
     9181 taatttccct gggaaacggg gccctccgca acgatttata ccggcagggg agagctatga
     9241 tcatgctggt gtacctggga atcgcccttt ttttcttttc ccttcttccc cacatcatct
     9301 atcttgtcgg tacatccctg gagaaaccgc cggaccctcg ggaacctccg ggggatcttc
     9361 ctcccatcac cctgatcctg tccgcataca acgaggagca gatcctcccc gcccgggtgc
     9421 gaaacctgat ggaatccacg tacccgaagg agaaggtgga aattatcctc atcgacgatt
     9481 tctcgggcga tcagacggcc cgggtggccg ccgaatccct cgggggatcc ggttttcgcc
     9541 accgggtcat ccggaacccg gagaggctcg ggacgaacag gtcgtacaac cgggccatcc
     9601 ccatggcgtc ccatcccatc atcgtgacca ccgatgccga cgtcttcttc gagccggacg
     9661 ccctggtccg ggtggtgacc cggctcgtga gcgatgatga tatcgtcgcg gtgtgcgggg
     9721 atctccgccc ctttcccagc gggacgagca cgaccgcgtt tgagtccgtg tatcggaatt
     9781 tcttcgggag gatgtgcgaa tgggagagcc ggatcgactc cacctatacc ttcaacgggg
     9841 ccctcgtggc cttcaggaag gaccgggtga accgcatcga ggataagaag ggatccgatg
     9901 atgcgaatac cgccttcgag gccatccgga gggggtaccg ggcggtgtac gagatccgtg
     9961 ccgtcgtcta tgagtacatc ccgccggatt tttccgccca gtatcgccag aagacccgtc
    10021 gcgcgaagag gctgatcgag gctaccctgg caaacctcga cctcctggga cacgatcgcc
    10081 ccttttcgcg gtacttctac ccgctccgga tcttcatgta cgtggtatcc cccgccctct
    10141 tcttcgcggc cctgttcctc ttcggggccg gagttctcct cctctcgccg gccctcttcc
    10201 tcatcctcgt cgcggtcttc gccctggtga cggtcgcctg gagaaggaac ttcgctgtcg
    10261 ccctcgtcct gaaccagttc tacctcctcg tcggcctcct ccgcctgccg accgagaccc
    10321 gtatctggga gagcacgacg aagcggatca ccgggtgatc acgaagcttt ccatgatcaa
    10381 ggagtcgacc ccgctggaga gccaggtgtg gagggcatcc gcgggatgga agaccatcgg
    10441 ctctccgtgg aggttgaagg aggtgttgag caccccccct cccgacgcgg gcccggtcag
    10501 gctgtaatct gctcgatcat tgatagagcc atgtccccac cgcaaccccc gcaccgaata
    10561 cgagcccgaa tactccaaga acagagataa ggaataccag ttcttccatt cctggatcgt
    10621 tataggctgg cagacgtgtt cgtgatagcg cgactgccaa agctaccgct ccgcctgcga
    10681 ggagaatcga tgccccggtt atggtaaccc accccctcgt cacgataccg gctatgaaaa
    10741 gaagggccag cacgagaaca acgccaccga tgaagagcca ctcctcccag aagaaatctt
    10801 caggccagcg catctttgct ttcccccctt gcgtcgaaca tcatcctgag ccagagttcg
    10861 gtacagatga ttcgccagaa ctccatggaa tacggagatc cccccttgac gaactccgtg
    10921 taactcgcga tgacccggtc tgcatcccag tacttccgcg cccggaaacc tgcagacgat
    10981 aagacctcgg agacgaactg gtggagctcg tttttcatcc agacctcctc cggggtgacg
    11041 aagcccattt tatccatcct gcatctcacg ggctccggaa cgaggccctt gatggctttc
    11101 ctgaggacgt acttcgtaac ccccttccgg atcttctgcg agaggggcag gacagccaga
    11161 aactcggcga cctgttgatc caaaaagggt actctcgcct cgatggagaa ggccatggag
    11221 ttcctgtcct cccagtggag gagacccgtg agattggttt cgaggatctc ccgtttcagg
    11281 gcttcatcca gactcccccc gtatcgtgag accacgggga tctctccacg gagggtggat
    11341 cgcctctgtt tccgaatgag aatctgagcg acggccccaa gaaagaatgg gccgtgaatg
    11401 cggaggcctc ccaccagttc gcgaagggcg gtgaggaggt gaaggcccct gacgaggccg
    11461 acgatgtagc tgccctggta ggcgagatag cccccgagct gctcatccgc tccctgacca
    11521 tcgaggacta ccttcaccga ttttgatgcg agcttcatta cctggtactg ggcggcgatg
    11581 gagagagagc caaatggctc gtcctgcatc cagagcaacc tctggagatc ttcgaggatc
    11641 tcccggggcc cgatggctgc ctttcttgag tccaccccgg atgcggccac cacctcatca
    11701 atgaaacgtt cctcgctgaa cctgatgtcc gagaagatta tggaaaaggt cttctgccag
    11761 ttcccgatgc tccggggatg ctctgccctg atcagccggt tgatgatgat cgcgatggtg
    11821 gatgagtcga cgccaccgga gaggcaggtg ccgaccggca catcgcttct caggtggatc
    11881 cggatggcgt ccgtgagcgt accctggaac gcgtgggcgg cttcctcatc ctgtattggc
    11941 cctggccggc ccggctcggg gttcatggtg agatcccagt acttctccgg aacacccccc
    12001 gcccggccga aggtaatcct gtgggccggt tggagctggt gaataccctc gaacatcgtc
    12061 ccccccgtgt gatcgaggac accccatgcc aggaaggtcg agaggagccg gtcgtctggc
    12121 ttcttgccca cccccgggtg tgccagaagt gccttgatct cagatgcgaa gaggagcgta
    12181 tcaccgacga tcgcgtagta gaatggtttg ataccgaacc ggtccctggc gcagaagagc
    12241 tcactcctcc gcgagtccca cagggcaaag gcccacatgc cattgaatcg tgccagacag
    12301 tccttgcccc attcctcgta ggcatggagc accacctcgc tatccccctg agaccggaag
    12361 gtatgacccc tcccagcgag ttcctccctc agttcgaggt agttatagat ttccccgtta
    12421 tagacgagcc agagcgcccc atcctcgttt gtcatgggtt gctctgctgc atcggtgaga
    12481 tcgatgatgg ctaggcgtct gtgggcgagc cctattgacc cgtccaggtg tataccgccc
    12541 ccgtccgggc cgcggtgcct gatccggtcg gacattgcct ccaggagcga cttgtcgaca
    12601 gttccattgt tctggttgaa gatccccgcg atcccgcaca tctggctcac cccccaactt
    12661 tatacgtcgt gccgccactc aggtttcgag gtgccaaacc ctgttttcac atggcagatt
    12721 ccgtaggtcg ccatcttaga tgctcacccc agccctttta ttcata
//
LOCUS       DATGDI010000024         3896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_2443, whole genome shotgun sequence.
ACCESSION   DATGDI010000024 DATGDI010000000
VERSION     DATGDI010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2443"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VKO45_01000"
     CDS             <1..484
                     /locus_tag="VKO45_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HMA04482.1"
                     /translation="ARGDAVALKSGRPHYEVPVEVLGASNRILLHALALARDNGCALQ
                     IHAEGGPCADVAEMAREAGLSPEKVVKHFATPETPLVPSLIARHEAIPALAREGRGFM
                     MESDFMDENSRPGSVIGPKSVPRFTRRLLESGELSEEAAHRIHAEVPSRTYGVEILSP
                     "
     gene            574..879
                     /locus_tag="VKO45_01005"
     CDS             574..879
                     /locus_tag="VKO45_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 domain-containing protein"
                     /protein_id="HMA04483.1"
                     /translation="MQKYVMFLKVHRTPDGEIVAVCDRELLNTTLVHREVEVHISEAF
                     YGNTPADEERVRAALATASNANLFGRRVIEIAVACGAVDRGCVVLIGGVPHAQVFRV"
     gene            884..1924
                     /locus_tag="VKO45_01010"
     CDS             884..1924
                     /locus_tag="VKO45_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="60S ribosomal export protein NMD3"
                     /protein_id="HMA04484.1"
                     /translation="MSIRESFCPLCGEPSAGGLCGKCRAGRTRWMEAAPRVEAITCPT
                     CGSRRTGAIWQETPVSREELAGELAVQAVRLVPEARDPEFRVTSRERSPNRTEAAVRV
                     RANLFGVPVEDEARIEIVWRGETCDRCSRISGGYFEGVIQVRADGRALTPHEQEVAAR
                     IAFEVEERLQAGGSRLSFISRMTDEEGLDIVVGQNAMGDQIAREVTGALGGRYTVHPK
                     LVGEKDGKRLFRITYSVRLPRLQRGDVVEVDGEYLEVRETGKRQLKVFQLATGSLATV
                     PPDAPYRVIGNVRDSLPALVAYLEEGVAGIIDPEDFTSREVRMPGWLKAKAGSEVLVI
                     PDRSLGRLVVVG"
     gene            1928..2806
                     /locus_tag="VKO45_01015"
     CDS             1928..2806
                     /locus_tag="VKO45_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HMA04485.1"
                     /translation="MRVRTIRFSDLSAIAGQAWVDPSRRPVVQDGVAYVPVREGEPHD
                     LVIPERKPYRGRGYQRLGDLILVHGKRPTQEELGEIVGWGKPRGVLWVRAIRGIERRP
                     VVETLHGTAGEVCHTESGCRFLLDPARVMYAMGNLTERRRVAELAAGSGRAERIADLF
                     AGIGYFTIPLARAGFHVHAMEISPVAHGYLVRNIRANRVEDRVDAGCGDCRDLLRGMY
                     DRFILGHFDALPCLTDALAHARSGSVLHIHTLRDETGRIGDLAREAGFSAAVTTRRVK
                     SYAPHTWHMVQDVTLS"
     gene            2773..>3896
                     /gene="coaBC"
                     /locus_tag="VKO45_01020"
     CDS             2773..>3896
                     /gene="coaBC"
                     /locus_tag="VKO45_01020"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HMA04486.1"
                     /translation="MAYGAGRDALVTRRTLEGRVIALGVTGSIAAVEVVGLAHALRRR
                     GAEIQPVMTPAAARIIHPDAVTYACGRPALTALSGMVEHVEYCGEGGKADLLLVAPCT
                     ANTLAKMALGIDDTPVTSFASTAIGRGMPVVIAPAMHASMYRHPAVTGHLKTLAGWGV
                     DVIGPRMEEEKAKLAAPETIVLHVERALMGGALRGKRVLVTSGPCAEPVDDVRVLTTR
                     STGKMGREMALEAYRLGADVTVVHQATLPCVRNIPARDAAEMRGAVLRVFGEEGADIY
                     VSAAAVSDFSPRRVEGKIRSGAAVNLRLDPLPKLLDEVLSAHHPAAIAFKLGRDEEVA
                     ARDLLGRGVGVVVINPPETMGAAESEATILTGSGTDRIKGS"
BASE COUNT          630 a         1253 c         1398 g          615 t
ORIGIN      
        1 cgcccggggg gatgcggttg ccctgaagag cggccggccc cactacgagg taccggtcga
       61 ggttctcggg gcgtcgaacc ggatcctcct ccatgccctg gcgcttgcgc gggacaacgg
      121 ctgtgccctt cagatccatg cagagggagg tccctgcgcc gacgtggcgg agatggcccg
      181 ggaggcaggc ctttcaccgg aaaaggtggt gaagcacttc gcgacccccg aaacccccct
      241 cgtcccttcc ctcatcgccc ggcacgaggc cattcccgcc ctcgcgaggg agggtcgggg
      301 cttcatgatg gagagcgact tcatggacga gaactcccgg cccgggtccg tcatcgggcc
      361 gaaatcggtc cccaggttca cccgccggct cctcgaatcg ggggagctct ccgaggaggc
      421 tgcacaccgg atccacgcgg aagtgccgtc ccggacctac ggggtggaga ttctctctcc
      481 ctaggtccac aggcaggggc acctgagggg atccgggtgc ggggcacctt tttcacccgc
      541 ggcgggggca gtacaccttt tttacctgcc ggggtgcaga agtacgtgat gttcctgaag
      601 gtccaccgga ccccggacgg cgagatcgtg gcggtatgcg accgggagct cctgaacacg
      661 accctggtcc accgcgaggt tgaggtgcac atctccgagg ccttctacgg gaatactcct
      721 gccgacgagg agcgggtgcg ggctgccctc gccaccgcct cgaacgcgaa cctcttcggc
      781 cgccgggtca tcgagatcgc cgtcgcctgc ggggcggtgg accggggttg cgtggtcctt
      841 attggcggcg taccccacgc ccaggtcttc agggtctaag gacatgagca tccgggaatc
      901 cttctgcccc ctctgcggcg agccctcggc ggggggtctc tgcggcaaat gccgggccgg
      961 acgcacccgg tggatggagg cggccccccg ggtcgaggcc atcacctgcc cgacctgcgg
     1021 gtcccggagg acgggggcga tctggcagga aactcccgtg tcccgggagg agctcgcggg
     1081 ggagctggcc gtccaggcgg taaggctcgt tcccgaggcg agggacccgg agttccgggt
     1141 gacctcccgg gagcggagcc cgaaccgcac cgaagcagcg gtccgggtga gggcgaacct
     1201 cttcggggtt cccgtggagg acgaggcccg catcgagatc gtgtggaggg gggagacctg
     1261 cgaccggtgc agcaggatct ccggcgggta cttcgagggg gtgatccagg tccgtgccga
     1321 cgggcgggcc cttacccccc acgagcagga ggtggcagcc cggatcgcct tcgaggtgga
     1381 ggagcggctc caggccgggg gatcccgcct ctccttcatc tcccggatga ccgacgagga
     1441 gggcctggac atcgtggtcg gccagaacgc catgggagat cagatcgccc gggaggtcac
     1501 cggcgccctc ggaggaaggt acacggtcca cccgaaactc gtcggggaaa aggacgggaa
     1561 gcgcctcttt cggatcacgt actccgtgag gctcccccgc ctccagaggg gggacgtggt
     1621 cgaggtggac ggggagtacc tggaggtccg tgagaccggg aagcggcagc tgaaggtctt
     1681 ccagctcgcg accggatccc tcgcgaccgt cccgcccgat gcgccgtacc gggtcatcgg
     1741 gaacgtgagg gactccctgc ctgcactcgt cgcctacctt gaggaggggg tggcgggcat
     1801 catcgatccc gaggacttca cctcgaggga ggtccggatg ccaggctggc taaaggcgaa
     1861 ggccggatcc gaggtgctgg tcatccccga ccggtccctg ggccggctcg tcgtcgtggg
     1921 gtgagggatg cgggtcagga cgatcaggtt ctccgatcta tcggccatcg ccgggcaggc
     1981 ctgggtcgac ccgtccagga gacctgtcgt ccaggatgga gtggcgtacg tcccggtgag
     2041 ggagggggaa ccccatgacc tggtcattcc cgagcggaaa ccctaccggg ggcgggggta
     2101 ccagcggctg ggcgatctca tcctcgtcca cgggaaacgc ccgacgcagg aggagctcgg
     2161 ggagatcgtc gggtggggaa aaccgagggg cgtcctctgg gtccgggcga ttcgggggat
     2221 cgagcgaagg cccgtggtgg agacccttca tggtaccgcc ggggaggtct gccacacgga
     2281 atccgggtgc cgttttctcc tcgacccggc ccgggtcatg tatgccatgg gaaacctcac
     2341 cgaacgccgg cgggtcgcgg agctcgccgc cggttcgggg agggccgaaa ggatcgcgga
     2401 tcttttcgca gggatcgggt acttcaccat cccccttgcg agagcgggat tccatgttca
     2461 cgcaatggaa atctcccccg ttgcccatgg ctacctcgtc cggaacatca gggcaaacag
     2521 ggtggaggac cgggtcgatg cagggtgcgg ggactgcagg gacctgctcc gggggatgta
     2581 cgaccggttt atcctgggtc acttcgatgc cctgccctgt ctcacggatg ccctcgccca
     2641 tgcacggagt gggtccgtcc tccacatcca cacccttcgt gacgagaccg gacggatagg
     2701 agatctcgcc cgggaggctg gtttttccgc ggccgtcacc acgcgaaggg taaagagtta
     2761 tgctccccat acatggcata tggtgcagga cgtgacgctc tcgtgacccg gagaaccctc
     2821 gagggaaggg tcatcgccct gggggtgacc ggatccatcg cggccgtgga ggtggtgggc
     2881 ctcgcccacg ccctgcgcag gcgtggtgcc gagatacagc ctgtcatgac cccggccgcc
     2941 gcccgtatca ttcatccgga tgcggtcacc tacgcatgcg gccgccctgc cctcaccgcc
     3001 ctctcgggca tggtcgaaca cgtggagtac tgcggcgagg ggggaaaggc cgacctcctc
     3061 ctcgtcgcgc cctgcacggc gaacaccctt gcgaagatgg ccctcgggat tgacgatact
     3121 ccggtgacgt cctttgcctc gacggctatc gggaggggga tgccggtcgt catcgccccc
     3181 gcgatgcatg cctccatgta ccggcacccc gccgtaaccg ggcacctgaa gaccctggcg
     3241 ggctggggtg tggacgtgat cgggccccgc atggaagagg agaaggcgaa gctcgccgca
     3301 cccgagacca tcgttctcca cgtcgaacga gccctcatgg ggggggctct ccgcgggaaa
     3361 cgagtgctcg tcacgagcgg gccctgcgcc gagcccgtgg acgacgtgcg ggtcctcacc
     3421 acccggtcga cgggaaagat ggggagggag atggccctcg aggcgtacag gctcggggcg
     3481 gatgtcacag tggtccacca ggcgaccctt ccctgcgtga ggaatatccc ggcccgggat
     3541 gccgcggaga tgcggggtgc cgtcctccgt gtcttcgggg aagagggggc cgacatttat
     3601 gtctcggcgg cggccgtctc cgacttctcc ccccggcggg tggagggaaa aatccggagc
     3661 ggcgcagcgg tgaacctccg gctggacccc cttcccaagc tcctcgacga ggtcctttcc
     3721 gcccaccacc cagccgccat cgccttcaag ctgggccgcg acgaggaggt ggcggcgcgg
     3781 gatctgctcg gccgcggggt cggggtcgtg gtgatcaacc ctccggagac catgggagca
     3841 gccgagagcg aagcgacgat ccttaccggg agcgggacgg acaggatcaa ggggtc
//
LOCUS       DATGDI010000025        13393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_176924, whole genome shotgun sequence.
ACCESSION   DATGDI010000025 DATGDI010000000
VERSION     DATGDI010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 13393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13393
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176924"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..939)
                     /locus_tag="VKO45_01025"
     CDS             complement(262..939)
                     /locus_tag="VKO45_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMA04487.1"
                     /translation="MKEVPKSKRYALGWYHEGVTHLSIQEYEEAMAFFDKALSAVPEQ
                     PDFLVGKGDVLFAMGRFDEALAQYQKAVTHDPGNYKAWLQIGTTLLKLERFTDAVEVF
                     RKLRSMNEYDGEVWLGLGIALFTLGQKMEAGDALRNAMRFKPNQPALYYFLALNEPDD
                     EQALRLLLRGYRIDSSNLDIIIELVNRLVNSGRKADAAAFFRKARAIDPGNPRVKALE
                     DVCAKKT"
     gene            complement(1100..2449)
                     /locus_tag="VKO45_01030"
     CDS             complement(1100..2449)
                     /locus_tag="VKO45_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HMA04488.1"
                     /translation="MDWLLPVALLGILAAYGLIAWYIRRNRIREDIITFYGPVMAIKT
                     SKVGLFDRFRPYARFLRIYGSVGIAMVVIVSVLMTTLILVLFPQEVAQPPQPEGIYSP
                     LNWLVIPGVNQFIPLTIPVILAIALTIAIHEFGHGILCRVEGILVKSMGVLVFVIPIG
                     FFVEPDEEELGRTPPVPKSRMFGAGIANNLVLGAACIGILVLLLGAVSPVPGPIIYAL
                     AGNSPAYNASIPLGSVIQAVDGRDVTSTGDIANILAGTKPGDPVSLTLVPAEVLRDLP
                     PGGPLPPGTAYHFNLGSAPDRSYGYMGVDYYATSEVSSILKGFGSVRGLLVFTILPID
                     LLYNQAPPLHLLLISMPDEAFFRVPFPLFWGVVHFFFWCGWFNFMVGTFNALPMVPFD
                     GGFIMKEGVEGFLRRIGRPQLADRVVLAISLVILALIILIVTIPTIAGVMAAIRPAG"
     gene            complement(2541..3176)
                     /gene="rnhB"
                     /locus_tag="VKO45_01035"
     CDS             complement(2541..3176)
                     /gene="rnhB"
                     /locus_tag="VKO45_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HMA04489.1"
                     /translation="MICGVDEAGKGAVLGPLVVAAVGCGSEEEARAIGARDSKTLSPG
                     RREEIYPEILARFRVATRVLTPEKIDARPRLSLNTCVARLHAEVVSELRPDLAILDAC
                     DVDAGRYRRTVAGFLPAPCRLVSEHEADRTHPVVGAASIVAKVLRDRAIADLAREHGE
                     IGSGYPSDPATIAYLREYLKAHRSPPPFARRSWATTRALVSELYQTRLPEF"
     gene            complement(3242..4444)
                     /locus_tag="VKO45_01040"
     CDS             complement(3242..4444)
                     /locus_tag="VKO45_01040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMA04490.1"
                     /translation="MKSRYDALVIGGGPGGAIAAGDLAGKGLSVCLVEKRPAIGAPVR
                     CAEGIGKDILKEFIEPDERWISAEIRGARIVAPDGTAMTLEQEMAGSKVGYILDRKVF
                     DRELVWQAACQGAEVHVKTRATGPIVENGAVKGAVLETCGQAREVRADVVIAADGVES
                     KFPRWCGVDSAVPLRELMSCAQYLLTDIDIDPFTTAFYLGNKVAPEGYAWVFPKGERT
                     ANVGIGISARRSRDGNRARDYLDRFVAAHFPEGKTIEYIVGGVPVCRPLPCTVADGLM
                     IIGDSARVADPMTGGGIYNAMHTGRLAAGVASESISAGDCSSAALMAYDQAWRHSKFG
                     KNLERNYRIKEYFITLSDEKLNAIVHSISKMNLKDFSVFNLVKELIRRNPRLLLELKG
                     LHDSLSAD"
     gene            complement(4428..4610)
                     /locus_tag="VKO45_01045"
     CDS             complement(4428..4610)
                     /locus_tag="VKO45_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA04491.1"
                     /translation="MLVIHRECCGYCGACVSVCPEAALELVDAYLTVSDDCLGCGVCA
                     RACPLGALEVTDEEPV"
     gene            complement(4716..7697)
                     /locus_tag="VKO45_01050"
     CDS             complement(4716..7697)
                     /locus_tag="VKO45_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMA04492.1"
                     /translation="MVSPAASDDHPVTRIYLPVYFIIASTLIPVIISVYCLSKGIFDI
                     FPHLFYIPIVLIAYAYPRKGIYGSVILGSIYLALVYLFAYPDLQVLGGATARFFFFIA
                     IGAVITLLARRMKHEEEKYHGIFDHSQDGVFLLSPDRKTVLNANPMAARLLASAPENL
                     TGLDFTSVWENPATMEAFGRAIGEGQSVAGLEGRILRRDGMIRDVLVSAGVLPDGKIA
                     CTVADITDRIEAARAVERERQELLDIIEFLPDATFVIDREKKVIAWNRAIETLTGVPK
                     VKVLGKGGYAYSVPFYGEARPALIDLIFEEIPGIESQFEWVERKGPILYGETFIPGMR
                     DGMGAHLWAKASPLLDSDGKIAGAIETIRDITERRMAEEALRESEANYRTVFNGANDA
                     IAIYDPETRLFTEVNQKAAEMFGFSPDEFTRLGFEDLSAGIPPFTREDALKKIAELWR
                     DVLGGKPRLFEWLARDRSGRTFWVELNLKVAPIWGEPRVLAVVRDVSSRKEAEEALRA
                     SEANYREIFSAVNEGIFIIDPSGGRILDANAKACEMYGRTVGEFRELDVDALGDTDHG
                     YILEGGKDVFTRAAAGEVQDFEWRAKDRAGRLFWVEVSVKRASIGGEDRILAVVRDIT
                     IRKQTEDLGRIQHKLAEHLGQVTAGDAAFQLATDALVRIEGIDASFACTLDEPGRGLH
                     PGHLHGFSLSNPPRLSCTMPGDLERELSGGKAVFLHAPSADPQIASIFAPEGIRAMAL
                     LPVLADRRILAVLVGCSRSLDEFSSLTRNGLETIGHQVGVNIARIRSQEALGKSEALY
                     RAVVEVQTEMISRFLPDGTHVFANEAYLRFYGKERDEIIGRKFIPPIPDPETAIVKRH
                     FASLSRENPVGTIEHRVILPTGEVRWTQWTDRAIFDGGGSLAEFQSVGRDITATKMME
                     EVERKAFSQIEKNIEQLAILGDHIRNPLAVIVGIADLEGGPTSEDIIKQAQIIDRIIT
                     QLDRGWIESEKIREFLKRSSR"
     gene            7963..8679
                     /locus_tag="VKO45_01055"
     CDS             7963..8679
                     /locus_tag="VKO45_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein"
                     /protein_id="HMA04493.1"
                     /translation="MATEIPAPDGKVAKRVLHFFWLTDYSASMGGKKIATLNQAIRES
                     LPLIQKAVAAHPQVQVMMRAIKFSDEAAWHVGPAPVTVEQFFWPELEADGLTATAKAI
                     RLLIPELSIEKMPRRGLPPVCILVSDGYCTDAEGEYDKAIGELNHTPWGMKSIRLAIA
                     IGDESDYNEPELLKFSNQEKVGILKAHTPEELLNFIKWASTEATLGSSRSKSQDLSGA
                     AGNVVLSPPPPTITSSTEPF"
     gene            8689..9717
                     /locus_tag="VKO45_01060"
     CDS             8689..9717
                     /locus_tag="VKO45_01060"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HMA04494.1"
                     /translation="MEETRETVPVSVPPIGWACGCTRRGSSHARDGRPCQDAHALWAS
                     SFLGEPLLVAAVADGHGGERHDLSHCGAALAVQAAVAEVIGSLPSREEGGGWRPVPPG
                     RGEEFHHRISARWREAVMTDAGGKIGFTADGEDPKDLLTRYGSTLLLALIDGSECWVG
                     QIGDGDILIVRPDGSVEAPLPKDETLLGTVTYSLSSRDAPALWRTGRFPIGAGGLLLL
                     ATDGLSDSFGDQDSEFRVFARSLSERVASFGIERIGSALPGWLDGYSLNGSGDDMTLV
                     LVHLNPSPGAGGEGQKPPAGEKAGDVTGSPPEGGDSGSAPADDVTTGSGPAGGITGSG
                     AGGKTDVP"
     gene            9707..11035
                     /locus_tag="VKO45_01065"
     CDS             9707..11035
                     /locus_tag="VKO45_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HMA04495.1"
                     /translation="MSLEAGQKVKAEMARITLTVRKKLGEGTQGEVYLVEGPHGQQAL
                     KWYKPAQATLDQMAAIRALVQAGPPRGAAGARFIWPLDLVTAAGAPQFGYLMRLIDTA
                     RFAELGEVQAHLKPVPGFRALTRISYQLANSYRALHLSGLCYRDISDGNLMFDPVAGE
                     ILICDNDNVGVNRQSRSQVWGTMEFMAPELIRGGADPSTETDLHSLAVLLFNLWCWHH
                     PFHGRMEYELRVWDIPAKKKVYGSPVFIFDPRDTSNALPSDPDYETAGERWGYCPRSL
                     RDLFTRAFTDGTRDPRRRVTEGEWQRLFLQFSDGIISCPRCRAENIWEPGEGTLTCWN
                     CSTSIPVPPRLLVTTSAGPHQVLLTEGATISREALGLGGRGDGDPAPVGQVVRNPADP
                     AIWGIRNLSQVVWRATFPSGQTLEIPNGKSVPLNPGARIDLGGVTGEIIA"
     gene            11051..12523
                     /locus_tag="VKO45_01070"
     CDS             11051..12523
                     /locus_tag="VKO45_01070"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HMA04496.1"
                     /translation="MADSNPREELARMVRHYGREIVTDPRRCEGLLRDTCRSCDREIF
                     ILASVQKKHIPEELLGLAGSVPGEIVVRRLVGRVTRELGFAEEHALWAVVSWALALSV
                     LSPEEAKSLEKRGRKRRSEGRAPPVPAGSYPPESIIVGQGREGDVRTLSDALERAAPG
                     TRIFVRPGIYPETLKIEKPIEIIGEGPAAGTILEGTGPLIQVKAETFSLHGVTLKGSP
                     DGEPGSPLVEILRGTTLVEDCVLSAGGVGISVTGGRTRGVLRRLDLHGFRTSAVLVSG
                     RASARLDGCRVSSSRSGITLADGAEAAIRGCRIEGGHYGIEFGARSGGTLEGSDITRY
                     AYAGVLVHDGAGPAVQKCTIHHGNFGIEVSDRGKGSLEDCDIAGNARGLFITRSGNPV
                     VRRCTIRDGQFGVGASEKGKGVFEECRVTGNQYAGVSSRRGAHPRLVRCQVTGNRDVG
                     VWVYEKAMATVEGCDLSGNARGPFTIEAGSKVTKKDTREG"
     gene            complement(12537..>13393)
                     /locus_tag="VKO45_01075"
     CDS             complement(12537..>13393)
                     /locus_tag="VKO45_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMA04497.1"
                     /translation="GKGGTGKTTISALLVEALIGAGEAPVLAVDADPNANLNEALGLP
                     LGETLGSMREDAFTKSIPPGMSRTGYIEYRFQQVLVESDGVDLLAMGRPEGTGCYCFA
                     NRLLSEAMDLLTRHYRFVVMDMEAGMEHFSRGTIGIPDILLVVTDPGARGVRTARRIH
                     DLAITLGIPKEKIFFVINRAGEAGKVTPDKAPWAAEAGVAGGGGPGMGTGGGSGIGVG
                     VASGGSSAAGTESLPDPSRILASIPDDPEVERADLDARPIVSIPAGSPARNAVRELAE
                     RVRSLCLR"
BASE COUNT         2323 a         4340 c         4474 g         2256 t
ORIGIN      
        1 cgcatcccac cgagatatcg ccatttcggt tctttttatc ggggtgctct cggggggcta
       61 cgccaccccg ccgcacgggc accccccggt ttccgggaat atctgaaatg aaggcttttg
      121 tgattcctgt ggatgaggat cccgacgagg cctagctatc ttgactgggg ggacgccaga
      181 gcggctgggg cgagcgaagc gagtcccaca ggagcgtcta cagtatgaaa acagtcaaca
      241 tgagggataa aatagaaatt ttcacgtctt cttcgcgcag acgtcttcga gggccttcac
      301 acgggggtta ccgggatcga tggccctcgc cttccggaag aaggccgctg catcggcctt
      361 tctccccgag ttgacgaggc ggttcacgag ctcgatgatg atgtccaggt tcgaggagtc
      421 gatccggtac ccccggagga gaagccggag ggcctgctcg tcgtccggct cgttgagggc
      481 gaggaagtag tacagggccg gctggttcgg cttgaaccgc atcgcgttcc tgagggcgtc
      541 cccggcctcc atcttctggc cgagggtaaa gagggcgatg ccgaggccca gccagacctc
      601 gccgtcgtac tcgttcatgg aacggagctt ccggaagacc tcgacggcat cggtgaagcg
      661 ctcgagcttg aggagggtgg tcccgatctg gagccaggcc ttgtagttcc cggggtcgtg
      721 ggtgacggcc ttctggtact gggcgagggc ctcgtcgaac cggcccatgg caaagaggac
      781 gtcccccttc cccacgagga agtccggctg ctcggggacc gccgaaaggg ccttgtcgaa
      841 gaaggccatg gcctcctcgt actcctggat ggagagatgc gtgacgccct cgtggtacca
      901 cccaagggcg tagcgcttgc tcttaggtac ctccttcatc tgacccgagc cccgaaccgc
      961 aagactcctg ttcgtacttc ttccatggac caatgatata tctatttctt tggatttttc
     1021 cgggcccgga gaaagttttc agaactttct ccctgaacgg gtcacatcct tccatcgggc
     1081 ccggtcccac cgggcccctt caaccggccg gcctgatggc cgccatcacg cccgcaatag
     1141 tggggatggt cacgatgagg atgatcaacg cgaggatgac gaggctgatg gcgaggacga
     1201 cccggtcggc gagctgaggc ctcccgatcc ggcggagaaa accctccacc ccctccttca
     1261 tgatgaaacc cccgtcgaag gggaccatgg ggagcgcatt gaaggtcccc accatgaagt
     1321 tgaaccatcc gcaccagaag aagaagtgga ccactcccca gaagaggggg aaggggaccc
     1381 ggaaaaaggc ctcgtcgggc atggagatga ggagaaggtg gagcgggggg gcctggttgt
     1441 agaggaggtc gatggggagg atcgtgaaga cgagaaggcc cctcactgac ccgaacccct
     1501 taaggatgga ggagacctcg ctggtggcat aatagtccac ccccatgtac ccgtacgaac
     1561 gatcaggggc agatcccagg ttgaagtggt aggcagtgcc cggtggaagg gggccgccgg
     1621 gggggagatc cctcaacact tcggccggga ccagcgtgag gctgaccggg tcccctggtt
     1681 ttgttccggc aaggatgttc gcgatatccc ccgtcgatgt gacgtcccgg ccgtctactg
     1741 cctggatcac cgaaccgagg ggtatgctcg cgttgtacgc cggggaattt cccgcgaggg
     1801 catagatgat gggccccggc accggcgaca ccgccccgag gaggaggacg aggatcccga
     1861 tgcaggcagc cccgagcacc aggttgttcg cgatcccggc cccgaacatc cgggacttcg
     1921 ggaccggggg tgtccgcccg agttcctcct cgtcgggctc gacgaagaac ccgatgggga
     1981 taacgaagac gaggaccccc atgctcttca cgagaatccc ctcgacacgg cagaggatac
     2041 cgtggccgaa ctcgtggatg gcgatggtga gggcgatggc gaggatgacc gggatggtga
     2101 ggggaatgaa ctggttcaca ccggggataa cgagccagtt gagagggctg tagatcccct
     2161 cgggctgtgg aggctgggcg acttcctggg ggaacaggac caggatcagg gtggtcatca
     2221 gtaccgatac aatgaccacc atggctatcc cgacggaccc gtagatccgg agaaagcgtg
     2281 cgtacggcct gaaccggtcg aataacccga cctttgaggt ctttatggcc atcaccggcc
     2341 cgtagaaggt gatgatatcc tcgcggatcc tgttcctgcg gatgtaccag gcgatcagcc
     2401 cgtaggcggc gaggattccc aggagggcga cgggtagcag ccagtccaca ctagatctct
     2461 gtttccggta cagataaaga atgggccctc cggaacgccg gaaaccggcg gccgggcctc
     2521 ggaacgaggg gggcggggca ctagaattcg ggcaggcggg tctggtacag ctcggaaacg
     2581 agcgcccgtg tggttgccca gcttcgccgg gcgaagggcg ggggagaccg gtgggccttc
     2641 aggtactccc tcaggtacgc gatggtcgcg gggtccgagg gatacccgct cccgatctcc
     2701 ccgtgttccc gtgcgaggtc tgcgatggcc cggtcccgga ggacctttgc cacgatgctc
     2761 gccgcaccca cgacgggatg cgtgcggtcc gcctcgtgtt ccgagacgag ccggcacggg
     2821 gcggggagga aacccgcgac ggtcctccgg tacctccctg catccacgtc gcaggcatcc
     2881 aggatggcga gatcgggtcg cagctctgac accacctcgg cgtgcagcct cgcgacgcag
     2941 gtgttcaggg aaagccgggg ccgggcatcg atcttctcgg gagtgaggac cctcgtggca
     3001 acccggaacc tggcgaggat ctcggggtag atctcctcac gcctgccggg ggagagggtc
     3061 tttgagtccc gggccccgat ggccctcgcc tcctcctcgc tcccgcatcc caccgccgcc
     3121 acgacgaggg ggccgaggac ggcacccttc cccgcctcgt ccacgccgca gatcacagga
     3181 gaaggtatgg gcacccgggt atttcaggat actggcggtt ggggaaaaag agggagaggt
     3241 tctagtcggc cgagagggag tcgtggaggc ccttcagctc gaggaggagc ctcgggttcc
     3301 tcctgatgag ctccttcacc aggttgaaga ccgagaagtc cttcaggttc atcttcgaga
     3361 tggagtggac gatggcgttc agcttctcgt cggagagggt gatgaagtac tccttgatcc
     3421 ggtagttccg ctccaggttc ttcccgaact tcgagtgcct ccaggcctgg tcgtaggcca
     3481 tgagggcggc cgaggagcag tcaccagcgg aaatactctc cgaggcgacg ccggcggcaa
     3541 gcctgcccgt gtgcatcgcg ttgtagatcc cgccgcccgt catggggtcg gccacccggg
     3601 ctgaatcccc gatgatcatg agaccgtcgg caacggtgca ggggaggggc cggcagaccg
     3661 ggacgccccc gacgatgtac tcgatggtct ttccctctgg gaagtgggcc gcgacgaacc
     3721 ggtccaggta gtcccgggca cggttcccgt cccgggaccg gcgggcggag atcccgatac
     3781 ccacgtttgc cgtcctctcc cccttgggga agacccaggc gtacccctcg ggggcgacct
     3841 tgttccccag gtaaaaggcg gtggtgaagg gatcgatatc gatgtcggtg aggaggtact
     3901 gggcgcagct catgagctcc ctcaggggaa cggcggagtc gacgccgcac caccggggga
     3961 acttcgactc gaccccgtcc gccgcgatga ccacgtcggc ccggacctcc ctcgcctggc
     4021 cgcaggtctc gagaaccgca cccttcaccg ccccgttctc gacgatgggc cccgtcgcac
     4081 gggtcttcac gtggacctcg gccccctgac aggcggcctg ccagacgagc tccctgtcga
     4141 agaccttccg gtccaggatg tacccgacct tggacccggc catctcctgc tcgagggtca
     4201 tggcggtccc gtcgggggcg acgatccggg cgccccggat ctccgccgag atccatcgct
     4261 cgtcgggctc gatgaactcc ttcaggatgt ccttcccgat tccctcggca caccggaccg
     4321 gggcgccgat ggcggggcgt ttctccacga ggcagaccga gaggcccttc ccggcgagat
     4381 cgcccgccgc gatggccccg ccggggcccc cgccgatgac gagcgcgtca taccggctct
     4441 tcatccgtca cctccagggc accgagcggg caggcccggg cgcagacccc gcagccgaga
     4501 cagtcgtcgc tcacggtaag atacgcatcg acgagctcga gggcagcctc ggggcacacc
     4561 gagacgcagg ccccgcagta gccgcagcac tccctgtgga tcacaagcat gggatcacat
     4621 tttcccggga attccattaa tctttaccct cgcgatgccg ttcttcaggt ggaggcgcga
     4681 gaccgtgaaa atgagaatcg ccgcagcgtg acgaatcacc gggaggacct ttttaggaac
     4741 tcccgtatct tctctgactc gatccagccc cggtcgagct gggtgatgat ccggtcgatg
     4801 atctgggcct gcttgatgat gtcctccgag gtcggccccc cttccaggtc cgcgatcccg
     4861 acgatgaccg cgagggggtt ccggatgtgg tccccgagga tggcgagctg ctcgatgttc
     4921 ttctcgatct gggagaaggc cttcctctcc acctcctcca tcatctttgt cgccgtgata
     4981 tcccgaccca cggactggaa ctcggcgagg gacccgcctc cgtcgaagat ggcgcgatcc
     5041 gtccactggg tccagcggac ctccccggtc gggaggatca cccggtgctc gatggtcccg
     5101 accgggttct cccgggacag ggaggcgaag tgcctcttca cgatggcggt ttcgggatcg
     5161 gggatggggg ggatgaattt tctcccgatg atctcgtccc gttccttccc gtagaaccgc
     5221 aggtacgcct cgttcgcaaa gacgtgggtc ccgtcgggga ggaaccggct gatcatctcg
     5281 gtctggacct ccaccaccgc ccggtagagg gcctcgctct tcccgagggc ctcctggctc
     5341 cggatccggg cgatgttcac cccgacctga tggccgatgg tctcgaggcc gttccgggtg
     5401 agggaggaga actcgtcgag ggaccgggag catccgacga ggacggcgag gatcctccgg
     5461 tcggcaagga cggggaggag ggccatcgcc cggatgccct cgggggcgaa gatggaggcg
     5521 atctggggat ccgcgctcgg agcgtggagg aagacggcct tcccgccgga aagttcccgc
     5581 tcgaggtccc cgggcatggt gcaggagagc ctcggggggt tcgagaggga gaagccgtgg
     5641 aggtggcccg ggtggaggcc gcggccgggc tcatcgaggg tgcaggcgaa gctcgcatcg
     5701 atcccctcta tccggacgag ggcgtcggtg gcgagctgga aggcggcgtc accggcggtc
     5761 acctggccga ggtgctcggc gagcttgtgc tggatccgcc cgagatcctc ggtctgcttc
     5821 cggatggtga tgtcccgcac caccgcgagg atccggtcct cccccccgat gctcgcccgc
     5881 ttcacgctca cctccaccca gaaaagccgg ccggcccggt ccttcgccct ccattcgaaa
     5941 tcctgcacct cgccggcggc cgcccgggtg aagacatcct tcccgccctc gaggatatac
     6001 ccgtggtcgg tgtcgcccag ggcatccacg tccagctccc ggaattcacc gacggtgcgc
     6061 ccgtacatct cgcaggcctt cgcgttcgcg tcgaggatgc ggccgccgga ggggtcgatg
     6121 ataaagatcc cctcgttcac cgccgagaag atctcgcggt aattggcctc cgaggcccgc
     6181 agggcctcct ctgcctcctt ccgggagctg acgtcccgga ccacggcgag gacacggggc
     6241 tccccccaga tgggggcgac cttcaggttg agctccaccc agaaggtcct ccctgaccgg
     6301 tccctcgcga gccattcgaa gagccggggc tttccaccga ggacatccct ccagagctcc
     6361 gcgatcttct tcagggcgtc ctcccgggtg aagggcggga taccggccga caggtcctcg
     6421 aatcccagcc gggtgaactc atcggggctg aacccgaaca tctcggcggc cttctggttc
     6481 acctcggtaa acaggcgggt ctccgggtca tagatggcga tggcgtcgtt cgcgccgttg
     6541 aaaacggtcc ggtagtttgc ctcggattcc cggagggcct cctcggccat ccgccgctcg
     6601 gtgatgtccc ggatggtctc gatagccccg gcgatcttcc cgtcgctgtc gaggaggggg
     6661 gatgcctttg cccagagatg cgcccccatc ccgtccctca tcccaggaat gaaggtctcg
     6721 ccgtagagga tcggcccctt cctttccacc cactcgaact gggactcgat cccggggatc
     6781 tcctcgaaga tcaggtcgat gagggccggc cgtgcctcgc catagaaggg caccgagtag
     6841 gcgtaccccc ccttgccgag gaccttcacc ttgggcacgc cggtcagggt ctcgatggcc
     6901 cggttccagg cgatgacctt tttctcccgg tcgatgacga aggtcgcgtc ggggaggaac
     6961 tcgatgatgt ccaggagctc ctgccgctcc ctctccacag ccctggctgc ctcgatccgg
     7021 tccgtgatgt cggcgacggt gcaggcgatc ttcccgtcgg gcagcacccc cgcggagacg
     7081 agcacgtccc tgatcatccc gtcccggcgg aggatccggc cctcgagccc cgcgacgctc
     7141 tgcccctccc cgatggccct cccgaaggcc tccatggttg cgggattctc ccagaccgag
     7201 gtgaagtcaa ggccggtaag attctcgggg gcagaggcaa gcagcctcgc cgccatgggg
     7261 tttgcgttga gaacggtttt ccgatcaggg gagaggagga agaccccgtc ctgagagtga
     7321 tcgaagatcc cgtggtactt ctcttcctca tgcttcatcc tccgggcgag gagggtgatg
     7381 accgccccga tggcaatgaa gaagaagaac cgcgccgtgg ccccccccag gacctggagg
     7441 tccgggtacg cgaagaggta gacgagagcg aggtagatgc tcccgaggat caccgagccg
     7501 tagatgccct tccggggata ggcatacgcg atgaggacga tgggaatgta gaagaggtgg
     7561 gggaagatgt caaagatccc tttcgagagg cagtacacgg agatgatgac ggggatcagg
     7621 gtagaggcga tgatgaagta caccgggaga tagatccggg tgaccggatg atcgtcggag
     7681 gctgcgggag agaccatagg tggatttaag ataaatatgt ttctacctat aaataccctt
     7741 tcttttttat ctcgttttgg atgaaggaat taaaaattta taatcgctat acttatcatt
     7801 ttcaagattt aaaccacatt tcattgtcct ctccttcagc cactcctgcc ccggggtttc
     7861 cggcctggca ggattccggc cgggcggttc ctgccggctg tccaatcctt taatatcgac
     7921 gagaaccgtt atacttgatc aagcacacgc gtgggagtta ccatggcaac cgagattcct
     7981 gcacccgacg ggaaagtggc aaaaagggtc ctccacttct tctggctcac cgactactct
     8041 gcctccatgg gggggaagaa gatcgcgacc ctcaaccagg ccatccgcga gtcccttccc
     8101 ctcatccaga aggccgtggc ggcccatccc caggtccagg tgatgatgag ggccatcaag
     8161 ttctccgacg aggccgcctg gcatgtgggt ccggcgccgg tcaccgtcga gcagttcttc
     8221 tggccggagc tcgaggccga cggcctgacc gcgaccgcga aggccatccg gctcctcatc
     8281 cccgagctct ccatcgagaa gatgccccgg cggggccttc ccccggtctg catcctcgtc
     8341 tcggacgggt actgcaccga tgccgagggg gagtacgaca aggccatcgg ggagctgaac
     8401 cacaccccct ggggcatgaa gtccatcagg ctcgccatcg ccatcgggga cgagtccgat
     8461 tacaacgagc ccgagctcct gaagttctcc aaccaggaga aggtcgggat tctcaaggcc
     8521 cacacccccg aggagctcct gaacttcatc aagtgggcct ccaccgaggc gaccctcggg
     8581 tcctcccggt cgaagtccca ggacctctcc ggggccgccg gaaacgtggt cctctccccc
     8641 ccacccccca ccatcacctc gagcaccgag cccttctagg agcgttccat ggaggagacc
     8701 cgggagacgg tgccggtcag cgtcccgccg atcgggtggg catgtggctg cacccggcgg
     8761 ggaagctccc atgcgaggga cggaaggccc tgccaggacg cccacgccct ctgggcctcg
     8821 tccttcctgg gggagcccct gctcgtggcg gcggtggccg acggccacgg gggggagcgg
     8881 cacgacctct cccactgcgg ggcggccctc gccgtgcagg ccgcggtggc cgaggtgatc
     8941 ggttcccttc cgtcgaggga agaagggggc ggctggcggc cggtaccacc gggcagaggt
     9001 gaggaattcc atcaccggat ctccgcacgc tggagggagg ccgtcatgac cgacgccggt
     9061 gggaagatcg gctttacggc ggatggagag gacccgaagg atcttctcac ccggtacggg
     9121 agcacccttc tcctcgccct gatcgacggc tccgagtgct gggtagggca gatcggggac
     9181 ggggacatcc tcatcgtccg cccggacggg agcgtcgagg cacccctccc gaaggacgag
     9241 accctcctcg ggacggtcac gtactccctc tcctcccggg acgccccggc cctctggcgg
     9301 acgggccggt tccccatcgg ggccgggggc ctcctcctcc tcgccaccga cggcctctcg
     9361 gactccttcg gggaccagga ttccgagttc agggtctttg cccggtccct gtccgagagg
     9421 gtggcgagtt tcggcatcga gaggatcggg tcggctctcc ccggatggct ggacgggtat
     9481 tccctgaacg ggagcgggga tgacatgacc ctcgtcctcg tgcacctgaa cccgtcgccg
     9541 ggagccgggg gagaaggtca gaagcccccg gccggggaaa aggcgggtga cgtcaccggg
     9601 tcccctcccg aaggcgggga ttccggatcc gcaccggccg acgacgtaac gacaggatcc
     9661 gggccagcag ggggcattac gggatccggg gcaggaggca agaccgatgt cccttgaggc
     9721 cggccagaag gtaaaggcag agatggcgag gatcaccctc acggtgagga agaagctcgg
     9781 ggaggggacc cagggggagg tgtacctcgt cgaggggccc cacggacagc aggccctcaa
     9841 gtggtacaag ccggcccagg ccaccctgga ccagatggcc gccatccggg ccctcgtcca
     9901 ggcggggccc ccccggggtg ccgccggggc ccggttcatc tggcccctcg atctcgtcac
     9961 cgccgccggg gctccccagt tcgggtacct gatgcgcctc atcgacacgg cacggttcgc
    10021 cgagctcggg gaggtccagg cccacttaaa accggtcccg ggcttccggg ccctcacccg
    10081 gatctcctac cagctcgcca acagctaccg ggccctccac ctctcgggcc tctgctaccg
    10141 ggacatctcc gacgggaacc tcatgttcga cccggtcgcc ggggagatcc tcatctgcga
    10201 caatgacaac gtgggggtga accgccagtc ccggagccag gtctggggca ccatggagtt
    10261 catggccccc gagctcatca ggggcggggc cgatccctcc accgagacgg acctccattc
    10321 cctcgccgtc ctcctcttca acctctggtg ctggcaccac cccttccacg ggaggatgga
    10381 gtacgagctc cgggtctggg acatcccggc aaagaagaag gtgtacggga gccccgtctt
    10441 catcttcgat cccagggata cgagcaacgc cctcccatcg gatcccgact acgagaccgc
    10501 gggggaacga tggggctact gcccgcggtc cctccgtgat ctcttcacac gggctttcac
    10561 cgacggtacc cgggatcccc ggcggagggt caccgagggg gagtggcaga ggctcttcct
    10621 ccagttctcc gacggcatca tctcctgccc gagatgccgg gccgagaata tctgggagcc
    10681 gggcgagggg accctcacct gctggaactg ttccacctcc atcccggttc ccccgaggct
    10741 cctcgtcacg acgagcgccg ggccccacca ggtccttctt accgaggggg cgaccatctc
    10801 ccgggaagcc ctcggtctgg gaggccgggg ggacggggat cccgcgcccg tcgggcaggt
    10861 ggtgcgaaac ccggccgatc ccgccatctg ggggatccgg aatctctccc aggtcgtctg
    10921 gcgcgcgacc tttccctcag ggcagaccct cgagatcccg aacgggaaat ccgtccccct
    10981 caacccgggg gcacggatag atctgggcgg ggtcacggga gagatcatcg cataggagac
    11041 aggaagcacc gtggccgaca gtaaccccag ggaggagctc gcccggatgg tccggcacta
    11101 cgggcgggag atcgtgaccg acccccgccg gtgcgagggc ctcctccggg acacctgccg
    11161 ttcctgcgac cgggagatct ttatcctcgc gagcgtccag aagaagcaca tccccgagga
    11221 gcttcttggc ctcgccgggt ccgttcccgg cgagatcgtg gtgcgccggc tcgtgggccg
    11281 ggtgacccgg gagctcggtt ttgccgagga gcatgccctc tgggcggtgg tgtcctgggc
    11341 ccttgccctc tcggtcctct caccggagga ggcaaaatcc ctggaaaaaa ggggaaggaa
    11401 gcgacggtcc gaggggagag cccctccggt accggccgga tcgtaccctc ccgagagcat
    11461 catcgtgggg cagggcagag agggcgatgt ccgcaccctc tcggatgccc tggagagggc
    11521 tgcccctggc acccgcatct ttgtccggcc ggggatctac ccggagaccc tgaagatcga
    11581 aaagcccatc gagatcatcg gggaaggacc ggccgcgggg acgatcctcg agggtacagg
    11641 ccccctcatc caggtgaagg cagaaacctt ctccctccac ggtgtcacgc tgaagggttc
    11701 tcccgacggg gaaccgggat caccccttgt cgaaatcctc cggggaacca cgcttgtcga
    11761 ggactgcgtt ctctcggcgg gcggggtggg gatatcggtc accggaggcc ggacccgcgg
    11821 ggtcctgaga aggctcgatc tccacggctt caggacctct gccgtgctgg tttccgggcg
    11881 ggcatctgcg aggctggacg ggtgccgggt ttcctcaagc cggtccggga tcaccctcgc
    11941 ggacggggcg gaggccgcga tccgggggtg ccggatcgag ggaggccact atggcatcga
    12001 gtttggcgcc cgttccgggg ggaccctcga ggggtcggac atcacccggt acgcctatgc
    12061 cggggtcctc gtgcacgacg gcgcgggccc ggccgtgcag aagtgcacca tccatcacgg
    12121 gaacttcggg atcgaggtga gcgaccgggg gaaggggagc ctcgaggact gcgacatcgc
    12181 ggggaacgcc cgcggcctct tcatcacccg ctcgggaaac cccgtcgtga ggaggtgcac
    12241 catccgcgac ggccagttcg gggtgggcgc gagcgagaag ggaaagggtg tcttcgagga
    12301 gtgccgggtc acggggaacc agtacgcggg ggtctcctcc cggcgggggg cccacccgag
    12361 gcttgtccgg tgccaggtca cggggaaccg ggacgtcggg gtctgggtct acgagaaggc
    12421 catggccacg gtggagggtt gcgacctctc ggggaatgcc cggggcccct tcaccatcga
    12481 ggcgggttca aaggtcacga agaaggatac ccgggaaggg taacccgggg cgggcttcac
    12541 cgtaagcaga gggagcggac gcgttccgcg agctcccgga ctgcgttcct cgccgggctc
    12601 ccggcgggaa tggagacgat gggccgggca tcgaggtctg cacgctccac ctccgggtcg
    12661 tcagggatgg aggcgaggat ccgggaggga tcggggagag attccgttcc agcagcggaa
    12721 ctgcccccgc tcgcaacgcc aacgccgatc ccagaacctc cccctgtacc catccctgga
    12781 ccgcccccgc ccgccacccc cgcctccgcg gcccatggtg ccttgtcggg agtgactttt
    12841 cccgcctccc cggcccggtt tatcacgaaa aagatcttct ccttcgggat accgagggtg
    12901 atggccaggt catggatcct ccgggcggtc ctgacccccc gggctccggg gtcggtgacg
    12961 acgaggagga tgtcggggat cccgatggtg cccctggaga agtgctccat gccggcctcc
    13021 atgtccatga ccacgaaccg gtagtgccgg gtgaggaggt ccattgcctc ggagaggagc
    13081 cggttcgcga agcagtagca gccggtcccc tcgggccggc ccatggcgag gaggtccacc
    13141 ccgtcggact ccacgaggac ctgctggaac cggtactcga tgtaccctgt ccgggacatg
    13201 cccggcggta tggacttcgt gaaggcatcc tcccgcatgg acccgagggt ctcgccgagg
    13261 gggagcccga gggcctcgtt caggttcgcg ttcgggtccg cgtccacggc gagcaccggg
    13321 gcctctcccg ccccgatgag ggcctctacg aggagggccg agatggtggt cttccccgtt
    13381 ccccccttcc ccg
//
LOCUS       DATGDI010000026         1582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_322571, whole genome shotgun sequence.
ACCESSION   DATGDI010000026 DATGDI010000000
VERSION     DATGDI010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322571"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VKO45_01080"
     CDS             <1..589
                     /locus_tag="VKO45_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HMA04498.1"
                     /translation="EKELMLELKLIADVGLVGFPNAGKSTLISKISEARPLIADYPFT
                     TLTPNLGVVDAGKDRSFVVADIPGLIEGAHLGHGLGVRFLRHIERTKILVHLIDISPY
                     TERDPIHDFEVIMAELEAFDPALARRPQILAANKIDLLGDDKNRLAGLKKLAARKKIA
                     LFPISALKNVGLKPLVRAMAKALESGGQKQGGEAA"
     gene            586..1245
                     /gene="nadD"
                     /locus_tag="VKO45_01085"
     CDS             586..1245
                     /gene="nadD"
                     /locus_tag="VKO45_01085"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HMA04499.1"
                     /translation="MKKRIGIFGGTFDPIHLGHIRAAEEVRAAFGLDKVLFIPSYIPP
                     HKQSGPSASAADRYRMVELACRGKDGLVPSPIEVEAKETSYSILTLNRVRGQFPEAWT
                     FFVLGVDAFLEIGTWREYERALAECLFIVTGRPGHALAAATEVLGGVLRERIHLGAEG
                     EFADESLFSRFGIFLLPIKALEISSTDIRNRLRQGASLAGLVPNSVEKYIRRHQLYSD
                     R"
     gene            1251..>1582
                     /gene="rsfS"
                     /locus_tag="VKO45_01090"
     CDS             1251..>1582
                     /gene="rsfS"
                     /locus_tag="VKO45_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HMA04500.1"
                     /translation="MAQEKKKSFTKRSLPADVKLAVETSQAKKAEGLCVLALGELSSF
                     TDYFLVMHGNSSRQNAAIADGIEGELRKAGIRPFSVEGRELGEWVLMDYGSFVVHVFS
                     PNARDYYSL"
BASE COUNT          313 a          466 c          487 g          316 t
ORIGIN      
        1 ggaaaaagag ctcatgctcg agctcaaatt gattgcggac gtcggactcg tcggattccc
       61 gaatgccggg aaatccaccc tgatctccaa gatttcggaa gccaggcccc tcattgccga
      121 ttatcctttc acaactctga ctccgaacct cggcgtcgtg gacgccggga aagaccggag
      181 cttcgtcgtg gcggatatcc ctggcctcat cgaaggcgcg catctcggtc acgggctggg
      241 cgtccgcttc ctccgtcata tcgaacggac gaagatcctg gtccatctca tcgacatctc
      301 gccgtacacg gaacgggatc ccatccacga cttcgaagtc attatggcag agctcgaggc
      361 ctttgatccg gcactcgccc gtcggcctca gatcctggcg gcgaacaaga tcgatctcct
      421 cggcgatgac aagaaccggc ttgccggcct caagaaactg gcggcgcgga agaagatcgc
      481 cctgtttccc atctcggcgc tcaagaatgt ggggctcaag cccctcgtcc gagcgatggc
      541 gaaggccctg gagtcgggcg gacagaagca aggaggcgag gcggcttgaa aaagaggatc
      601 gggatcttcg gagggacatt cgatcccatc catctcggcc atatcagggc ggcggaggag
      661 gtgcgggcgg cgttcgggct ggacaaagtc ctgttcattc cgtcctacat cccgccccac
      721 aagcagtccg ggcccagcgc ctcggccgcc gaccgttacc ggatggtcga actcgcctgc
      781 cgggggaaag acgggctcgt tccgtctccc atcgaggtcg aggccaagga gacctcctat
      841 tccatcctga ccctgaaccg ggtgaggggc cagttccccg aagcctggac attttttgtc
      901 ctcggcgtgg atgctttttt ggaaatcggg acctggcggg agtatgaacg tgccctcgcg
      961 gaatgccttt tcatcgtcac ggggcgacca ggccatgccc ttgccgccgc gaccgaggtc
     1021 ctgggcggtg tccttcgcga aagaattcat ctgggtgcgg agggcgaatt cgcggacgag
     1081 tcgctctttt cccgtttcgg gatttttctg ctgccgatca aggccttgga gatctcctcg
     1141 acggatatcc ggaaccggct ccggcagggg gcgagcctgg cgggcttggt ccccaactcc
     1201 gtcgaaaaat atatccggcg tcatcaactt tattcggatc ggtaggaacc atggcccaag
     1261 agaaaaaaaa gagttttacg aagcggagcc ttccggcgga cgtgaagctg gctgtcgaga
     1321 cgagccaggc gaaaaaagcg gagggcctct gcgtcctggc cctcggcgaa ctcagctcct
     1381 tcacggatta ttttctcgtc atgcacggga attcatcgcg ccagaacgcg gccatagcgg
     1441 acggcatcga aggggaattg cggaaggccg ggatcaggcc gtttagcgtg gaaggccggg
     1501 agctcgggga atgggtcctc atggactatg gctcgtttgt cgtgcacgtg ttttctccaa
     1561 acgcgcggga ttattactct ct
//
LOCUS       DATGDI010000027        11549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_2648, whole genome shotgun sequence.
ACCESSION   DATGDI010000027 DATGDI010000000
VERSION     DATGDI010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 11549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11549
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2648"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..673)
                     /locus_tag="VKO45_01095"
     CDS             complement(89..673)
                     /locus_tag="VKO45_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HMA04501.1"
                     /translation="MPYDDLISALKASATERITEIQDRAQAEAQKILKEAEERAQSTR
                     SASLEEAAREVRLERSKLMAKAGAEKRMALARVKDDLFQQVFARAVQQMASARNHPGY
                     RASFQKMLSEVTEELRGEEIRVHIDPHDEVLCREILGEMKRNCEIVTDITTVGGLNAT
                     SADERLMVFNTFESRLQRAKELMISEIMSALYGD"
     gene            complement(749..967)
                     /locus_tag="VKO45_01100"
     CDS             complement(749..967)
                     /locus_tag="VKO45_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HMA04502.1"
                     /translation="MVDWGIPIGAGIAFGLGALGTGLAQSKIGSAGAGTIAERPETFG
                     YMVVLVAIPETLVILGFVVASMIMLMLK"
     gene            complement(1223..3139)
                     /locus_tag="VKO45_01105"
     CDS             complement(1223..3139)
                     /locus_tag="VKO45_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HMA04503.1"
                     /translation="MTKVQVVGPKKDLQTVVDILYHTGTVHLEDISKSITPGDSILRR
                     LDMVKGGETATLLAKIGGIILALPKTEGYTGEQARLGEEFQRMSHEELTARANQLIGQ
                     LESVTRDLATKKSDLELNLGSLSRYEKIIDKLQPLESQLPALEGFEVTVLLIQKEFRE
                     VLEFIQSALREITRNQFELISADVDATTLATVLIFNKRYSEQVHSFIFSQNVNEVRLP
                     PEYMGKPFYEILTLIGDRKKEAVKEIAAIDIQLQKLATTWYQELSVLQRILGDRNAEL
                     SVFSKFGQTEYTFVIMGWVPRKYLKKTKKDLADTFGARVIVNEIDVPAEKMDAAPTFY
                     DNPWFVRPFEFLIQMISPPKYREVDPSPLIAIFFPIFFGLMVGDIAYGLIILAFALIM
                     KRKYKTYEWLQYLMNILAISSVPSIFFGFIFGEFFGNLGEMLGWIHPLTFLGITWNRI
                     EVIIPLLILAISIGVFHVFLGLAIGVVNALTKGDRHHACEKCGMIIAISGLLLTIGAV
                     AGAIPEIMVYPGVAMLLVALPLIIYGGGFFGAFEIMSTVGNILSYARIMAIGMASVVL
                     ALVANTLGGSIDVVLVGILIAAMLHILNIILAMFSPFLHSFRLHAVEFHSKFFEGGGT
                     LYSPFKRGMEGKNE"
     gene            complement(3154..3474)
                     /locus_tag="VKO45_01110"
     CDS             complement(3154..3474)
                     /locus_tag="VKO45_01110"
                     /inference="COORDINATES: protein motif:HMM:NF028309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit subunit G family protein"
                     /protein_id="HMA04504.1"
                     /translation="MTTERSLLQKIREKELEINVQVDDARRGAEETIAKARKEAAEIL
                     KNAGIEAKAGAQDLTRREMETVTREIEAVRSAAAGEVKTLKEKGERNLPQAIEKITKV
                     VALE"
     gene            complement(3486..3665)
                     /locus_tag="VKO45_01115"
     CDS             complement(3486..3665)
                     /locus_tag="VKO45_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04505.1"
                     /translation="MAMDSTVEKLVKNAVERCKERVLSTKKRVVERGLNQSAQTGVQG
                     EPPCITGSMKRLRGL"
     gene            complement(3824..4309)
                     /locus_tag="VKO45_01120"
     CDS             complement(3824..4309)
                     /locus_tag="VKO45_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMA04506.1"
                     /translation="MRYPIGDRILLIRGDHSFLLVGRALSEFPLYIETFEEEHRETIS
                     EDDLIAVSAPEGGDTEPARMLLELVRRYHMPLLVLPKNHPGSGRLPLVVSVAPSLRLS
                     CDIRRGTHPEQHLICSSDELAGITLRGSKGGADLDGIPSGVRVSYLDGRAEGKPAHEH
                     P"
     gene            complement(4306..4458)
                     /locus_tag="VKO45_01125"
     CDS             complement(4306..4458)
                     /locus_tag="VKO45_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04507.1"
                     /translation="MCSVGGPMDVDYKERVKGKDYKCRSCGEKFKGVGKHPICPSCQS
                     DDVAPE"
     gene            complement(4930..6846)
                     /locus_tag="VKO45_01130"
     CDS             complement(4930..6846)
                     /locus_tag="VKO45_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HMA04508.1"
                     /translation="MNAKASVSSWIRGRRDSYAWLHNDLISARSGVTIEHYLYQTFVI
                     TILSGIFCAFAGFITADLIANLPVSTQVYDVFGVNRVFPQAVPMLIFRGVVTIAAFIA
                     GSVLGYKVMTGFPALLKKNRATRINLTLHSAVAYMYAMQRGGAELSAIFASLADNANI
                     FGEVAMEFRQFVRDVDYFGADLVTALKHLGETTPSEKFRDFIQDLLSVIDSGAGLGEF
                     FSGRVRTYQEEARFELKQFITTLEFVAETYVTLFVAGPLFLIIIMVVMGLIGGMGLTT
                     LAVVCYALIPIGSILFIVFLSMISDKVEGVERYVKAKELHEYFDVAVVKKIGEEAMFA
                     QLAFYDRIRRFRDFLRNPFNAFVLIPVRTLYLTVPIGIIYLILAFLSVPYYLDLQTKV
                     MILDDHVVIAGLIVIIPFAIFYQLWKHKVMGIEAAIPEFLDRMSGVQEVGLTIAQGLT
                     LMVRTNLGILSYEIKRIKRDMDWGANVEDALVRFEQRVRTPSIARTVTLITKASYMSG
                     DIGEILKIAASDARMSEILKKERSTSMLIYLAIIYLAFAVFIFVVGVIVTMFLPILLK
                     GGAQTVSTAGKLVTGQMITADSFRLLVYHATLIQGLFSGLLAGHMGEGSLTAGVKHVC
                     ILLIIALVAFNTFI"
     gene            complement(6843..8462)
                     /locus_tag="VKO45_01135"
     CDS             complement(6843..8462)
                     /locus_tag="VKO45_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HMA04509.1"
                     /translation="MQLPEVLRRLRLKDAYHLFGRRLGTWRESEKVYDPDKHGILVTP
                     DLGPGFEVVDVYWVERGLSLVCIALNQKNHQNEYILFEPPLSSFEYELLERLYEDLRD
                     VLLLNEEEIRGDKRQILLSLVEELLGEYGVKVDLPSLFRLQYFLVRNFLGWSRIDALM
                     KDPQIEDISCDGVKIPIFLYHRRFHNVKTNIVFDEDHLNSLAITLAQRSGKHISIGTP
                     ILDATLPDGSRLQLTFSKVVTSRGTSFSIRKFREEPFTPIELLEHGTFPVDAMVYFWL
                     AVENNKSLLFIGGTASGKTTSLNAVSHFIPPLAKVVSIEDTREITLERENWIASVTRD
                     AVIDGSSGMISMFDLLKAAMRQRPEFIIVGEVRGPEAQTLFQAMNTGHTTFSTLHAAD
                     VDAAIHRLENEPLAVPRNMLQALDIVSIQALIYRGTERVRRCQEIVEIMGLDQATQNI
                     QVNTVFSYDPVTDTFTYSGRSQVYNQIMASRGWSTEVLEQEILRRGKILGAMREQGMK
                     DYHSASQVFQSYAIKPDEVMENLGDLRKLIG"
     gene            8628..9533
                     /locus_tag="VKO45_01140"
     CDS             8628..9533
                     /locus_tag="VKO45_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04510.1"
                     /translation="MGVQVAGRMPTGISSLDPVLDGGIPPGSVVLLEGEIGAGSQEFV
                     YSSLIYLSILRQRQDAGPGARVPDTISYITFTRLKQDVLSEIGLSFAPTLTQHLDGSV
                     NFSDLSTHYFDSSVVPSDWYCTDGSDECEKKRKQSPETMFSELSTHVSPGRKNTLLKL
                     LYLTLSLANPKKAKKGEGSSLIILDSLTDLATQFSEPERWRELTAFLRGLQRVTKVWN
                     STVFLLLTKGVLEPSREKEIADCVDAVALFQWEETVAQRRQRTMFFQKFRGVMPHLEE
                     RDLVKFVVRISPGSGFEVSNIRVVV"
     gene            9530..10231
                     /locus_tag="VKO45_01145"
     CDS             9530..10231
                     /locus_tag="VKO45_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KaiC domain-containing protein"
                     /protein_id="HMA04511.1"
                     /translation="MNSDGRISLGIEGLDAMLGGGLIKPSICALIGTYGTGKTTLSIQ
                     FITEGLANKEKCIYISLDERVEMLKQIMKSRGRDVGSYVNKSLFLIKLDPTNFNLMIN
                     SIKEELFELIEKTGATRVVIDPISLFEGTFQSSVDRRLELIRFIEMMRDQPCTLLMTS
                     ETESNNPLMSRYGLIEYLVDTVILLRYYRPSDLTAVHLAVEVVKMRQSRHSREIKPFE
                     IMQDQILVYSGANVF"
     gene            complement(10626..>11549)
                     /locus_tag="VKO45_01150"
     CDS             complement(10626..>11549)
                     /locus_tag="VKO45_01150"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HMA04512.1"
                     /translation="QGMLEGALKHPESKITSGSGSLSRSEATIANSHGIQYTAERTEV
                     SCSLETICGQSTGSEFAHSPFLEIDPRWVGERAAFFAWHSSKGIPVETCRCDVLLSPI
                     ALAQLLGYIVVPALSGRNVHAGRSRLASLLGERCMDPRIDLYDDPFARGMGATAWDAE
                     GLPAARLDFVKAGVLTSFAYDLKTAYRYGAKPTASATRGGPGGGPSIGVHNLVLDGQR
                     EEIAMERALFVHDVVGAHTANPVSGDFSVECQNPIWVEDGGFQEPVRKAMLSGNFFDA
                     LREIGGIAPGSRIVGNSILPCVRFRGLQVIG"
BASE COUNT         2246 a         3515 c         3475 g         2313 t
ORIGIN      
        1 tttgaggatg aggctctcga ggatctccgg cccgagaagg cggctcttca tccccctgat
       61 ccgggcgttg acatagccgt aatccacgct agtcgccata gagtgccgac atgatctctg
      121 atatcatcag ttcctttgcc cgctgcaacc tcgactcgaa ggtgttgaag accatcagcc
      181 gctcgtcagc cgaggtggca ttcaggcctc ccacggtggt gatatcggtg acgatctcgc
      241 agttccgttt catctcccca aggatttccc tgcaaagcac ctcgtcatgg ggatcgatat
      301 gcacccggat ttcttccccc ctgagctctt ccgtcacctc gctgagcatc ttctgaaacg
      361 atgcccggta cccggggtga ttccgtgccg atgccatctg ctggacggca cgggcaaaca
      421 cctgctggaa gaggtcatcc ttcaccctcg cgagggccat tcttttctcg gccccggcct
      481 tcgccatgag cttgctcctc tccagccgaa cctcccgggc tgcctcctca agggatgccg
      541 aacgggtgct ctgtgccctc tcctctgcct ccttcaggat cttctgtgcc tctgcctgtg
      601 cccgatcctg gatctccgta atcctttctg tggcgctcgc cttgagggct gaaatcaggt
      661 catcatacgg catggctcca ccagtttttt cctgttctgc tcgttcatcc acgccgggat
      721 tcccgcggcg cgaatactcc cactccgctc acttcagcat gagcatgatc atggatgcaa
      781 ccacaaagcc cagaataacg agcgtttcag gaatcgcaac caggacaacc atgtagccga
      841 aggtctccgg cctctctgca atggttcctg cccccgctga tccgatcttg gattgcgcaa
      901 gtcctgttcc gagcgcacca agaccaaatg cgatccctgc gccgatgggt attccccagt
      961 ctaccatacc aaagctcacc aacctctact cttggaatca gtcatcgtga atattaacct
     1021 tgcttaccag cgattacgac gggctcattc ttcggtgcgc cctcgtggct tcgatgaatt
     1081 tctatcccat cctcctcccg gttttcctcc tccttcaggt cacttcacca tcgcgacccc
     1141 cttcaggaga gagagaacct gcggtccgga actcggatca ccctcctccc tgcaatcgcg
     1201 ggagggtgtc cttgcggtgc acctattcat ttttaccttc catgcccctc ttgaacggcg
     1261 aatacagggt gcctccaccc tcgaagaact tggagtggaa ctccaccgca tggagacgaa
     1321 aggaatggag gaaggggctg aacatggcaa ggatgatgtt caggatgtgc agcatcgccg
     1381 caatgaggat cccgacgagc actacgtcta tcgagcctcc cagcgtattt gccacgagcg
     1441 cgaggaccac ggaggccatg ccgatggcca tgatacgggc ataggaaaga atgttgccca
     1501 ccgtcgacat gatctcgaac gccccgaaga atccaccgcc atagatgatg agcgggagcg
     1561 cgacgaggag catggcaacc cccgggtaca ccatgatctc ggggatcgcg cctgccaccg
     1621 cacctatggt aagaagcagg cctgaaatgg cgatgatcat cccgcacttc tcacaggcat
     1681 ggtgccggtc ccccttcgtc agggcgttga cgaccccgat ggcgaggcca aggaagacgt
     1741 ggaagacccc gatggagatg gcgaggatga ggagcgggat gatcacctca atgcggttcc
     1801 acgtgatgcc gaggaaggtc aacgggtgga tccagcccag catctcgccg aggttcccaa
     1861 agaattcccc gaagatgaag ccaaagaaga tgctcggaac cgacgagatg gcgaggatat
     1921 tcatcaggta ctggagccac tcatacgttt tgtacttccg cttcatgatg agcgcgaagg
     1981 caaggatgat caggccgtag gcgatgtccc ctaccatgag gccaaaaaag atggggaaga
     2041 agatggcgat gagcgggctc gggtccactt cgcggtactt cggcgggctg atcatctgga
     2101 tcaggaactc aaaaggtctg acgaaccagg ggttatcgta aaaggtgggc gccgcatcca
     2161 tcttctcggc cgggacatcg atctcgttca cgatgacccg ggccccgaag gtatcggcga
     2221 gatccttctt tgtctttttc aggtacttcc tcgggaccca tcccatgatc acgaaggtgt
     2281 actcggtctg cccgaatttt gagaagacgc tcaattccgc gttcctgtcc ccgaggatcc
     2341 tctgcaatac ggataattcc tggtaccagg ttgtcgcgag cttctgcagc tggatgtcaa
     2401 tggcggctat ctccttgaca gcctcctttt tccgatcccc gataagcgtc aggatctcat
     2461 agaagggctt gcccatgtac tcgggaggca ggcgcacctc gttgacattc tgggagaaga
     2521 tgaaggaatg gacctgttcc gagtatcgtt tgttgaagat gagaaccgtg gcgagggtgg
     2581 tcgcgtcgac gtcggccgag atgagctcga actggtttct cgtgatctcc cggagtgcgg
     2641 actggataaa ctcgagcacc tcccggaact ccttctggat caggaggacg gtgacctcaa
     2701 acccttccag cgcaggaagc tggctctcca ggggctggag cttatcaatg atcttctcgt
     2761 atcgggagag ggatccgagg ttcaattcca gatcgctctt cttcgtggcc aaatcccggg
     2821 ttacggattc aagctgacca atcaactggt ttgccctcgc ggtcagctct tcatggctca
     2881 ttctctggaa ctcctcacca agacgcgcct gctcccccgt atagccctcg gtcttcggca
     2941 aagcgaggat gatgcccccg atcttcgcga gcagtgtggc cgtctcaccc cccttcacca
     3001 tgtcaagccg cctcaggatc gagtctccgg gggtgatgga cttggagatg tcttccaggt
     3061 ggacggtgcc ggtgtggtac aggatatcga cgaccgtctg caggtccttc ttcggcccca
     3121 cgacctgaac cttggtcatc ttctgaagca tacctattcc agagcaacaa ctttggtgat
     3181 tttttctatt gcctgaggca gattccgttc tcccttctcc ttcagggtct tgacctcgcc
     3241 tgctgcggcg gatctcactg cctcgatttc cctggtaacg gtttccatct ctctccgggt
     3301 gagatcctgc gcccctgcct ttgcttcgat ccccgcgttc ttcaggatct cagccgcttc
     3361 ctttcgtgcc ttggctatgg tttcctcggc ccctctcctc gcatcgtcca cctggacgtt
     3421 gatctccagc tccttctccc gaatcttctg gagtaacgag cgctcggttg tcatttcatc
     3481 atccctcaca ggccccggag ccgtttcatg gaaccggtga tgcagggcgg ctctccctgg
     3541 acgccggtct gggcggattg gttgagaccc cgctcaacaa cccgcttttt tgtcgaaaga
     3601 accctctcct tgcagcgctc gacggcattc tttaccagct tctcgacggt ggagtccatg
     3661 gccatgccgg gttgtcttga gcccctctat tggacgcttt tcttagtgtt ttttgccacg
     3721 ataatggtat cgttaatccg agcctcatat gaggtggaaa cgtccttctt cctatgataa
     3781 cggagtcgga tggccggcca ggaaggggat gggaatgaaa atttcagggg tgttcatggg
     3841 ctggctttcc ctctgcgcgc ccatccaggt acgagactcg gacgccggaa ggaattccat
     3901 ccagatcagc ccctcccttc gatccccgga gggtgatccc ggcaagttca tctgacgagc
     3961 agatgaggtg ctgttcgggg tgagttcccc gcctgatatc gcaggaaagg cggagggagg
     4021 gggcaaccga taccacgagg ggaagtcgcc ccgatccggg gtggttcttc gggagaacga
     4081 ggaggggcat gtggtaccgc ctcaccagct cgaggagcat ccgggccggc tccgtatccc
     4141 ctccctcggg ggccgaaaca gcgatgaggt catcctcgga gattgtttcg cggtgctcct
     4201 cctcgaaggt ctcgatgtac aggggaaatt ccgagagggc tctccccacg aggagaaaag
     4261 agtggtcgcc ccggatgagg aggatccggt cgccgatggg atatctcatt cgggtgctac
     4321 gtcgtcagac tggcagctgg ggcagatggg gtgcttcccg acccccttga acttctcccc
     4381 gcagctccgg cacttgtagt ccttgccctt cacccgctcc ttgtagtcga cgtccatggg
     4441 cccgcccact gagcacatcc aacatcacct cactacatgg tgcctgcgta acgtcttaag
     4501 gataccggta cccgggagat ccaaaacggg gggggcccaa gaccagaatc cgttatacct
     4561 tttatccagg acgctcctgg gggaagggag ggttcgccag aacccgacct ggagcacccc
     4621 gccatcaggc ggtgtgcgac cgggaggttt cgcaagaggg agggttcgta ggacccgacc
     4681 tcgagcgacg aagtcgcgag tatgccccgc tcacccccgc cactgcggcg ccccccgcgt
     4741 ggggataggc cgttattcca taaaccgcca gcttcgtgag gatatcagaa acgggggggt
     4801 tgcccgtgcg gcggggggcg ggtgagcccc ccgagagcac cccaataaaa tgaggtcaaa
     4861 tgatgtcccg tcgtttcgat tcttgaatga acctaataca tcatcgggct ggtgaaattc
     4921 cctaaagctt cagatgaagg tgttgaaggc cacgagggcg atgatgagga ggatgcagac
     4981 atgcttcact cccgccgtga gggagccctc ccccatgtgc ccggcgagga ggcccgaaaa
     5041 gaggccctgg ataagggtcg cgtggtacac gaggagcctg aaggagtcgg cggtgatcat
     5101 ctggccggtg accagtttgc ccgcggtact cacggtctgc gcccctccct tcaggaggat
     5161 gggaaggaac atggtgacga tcaccccgac cacgaagatg aagacggcga aggcgaggta
     5221 gatgatggcg aggtagatga gcatcgaggt ggatcgctct ttcttcagga tctcgctcat
     5281 ccgggcatcc gaggccgcga tcttcaggat ctccccgata tccccgctca tgtagctcgc
     5341 cttcgtgatg agggtcaccg tccgggctat ggagggggtc cgcacgcgtt gctcgaaccg
     5401 caccagggca tcctcgacgt tcgcccccca gtccatgtcc cgcttgatcc tttttatctc
     5461 gtaggagagg atcccgaggt tcgtccttac catgagggtg agcccctggg cgatggtgag
     5521 gcccacctcc tgcacgccgg acatacggtc caggaactcg gggatggccg cctcgatccc
     5581 catgaccttg tgcttccaga gctggtagaa gatggcgaag gggatgatga cgatgagccc
     5641 ggcgatgacc acgtggtcgt ccaggatcat caccttcgtc tggaggtcca ggtagtaggg
     5701 gacagagagg aaggcgagga tcaggtagat gatcccgatg gggacggtga ggtagagggt
     5761 gcggacgggg atgaggacaa aggcgttgaa gggattccgc aggaagtccc ggaaccggcg
     5821 gatccggtca tagaaggcga gctgggcgaa catcgcctcc tcgccgatct tcttcaccac
     5881 cgcgacatcg aagtactcgt ggagttcctt ggccttcacg taccgctcga ccccctcgac
     5941 cttatccgag atcatggaga ggaagacgat gaagaggatg gaaccgatgg gaatgagggc
     6001 atagcagacc accgcgaggg tggtgaggcc cattccccca atgagcccca tgaccaccat
     6061 aatgatgatc aggaagaggg ggcccgcgac gaagagggtg acgtaggtct cggccacgaa
     6121 ctcgagggtg gtgatgaact gtttcagttc gaaccgggcc tcctcctggt acgtgcggac
     6181 ccggcccgag aagaactccc cgagcccggc cccgctgtcg atgaccgaga ggaggtcctg
     6241 gatgaagtcc ctgaacttct cggacggggt ggtttcgccc aggtgcttga gggcggtgac
     6301 caggtcggcc ccgaagtagt ccacgtcccg gacgaactgc cggaactcca tggccacctc
     6361 gccgaagata ttcgcattgt cggcgaggga ggcgaagatg gccgagagct cggcgccccc
     6421 cctctgcatg gcgtacatgt aggcgacggc ggagtggagg gtgaggttga tccgggtggc
     6481 cctgttcttc ttcaggaggg cggggaagcc ggtcatcacc ttgtagccga gcaccgatcc
     6541 cgcgatgaag gccgcaatgg tcacgacccc ccggaagatc agcatgggaa ccgcctgcgg
     6601 gaagacccgg ttcaccccga agacatcgta gacctgggtc gacacgggaa ggttcgcgat
     6661 gaggtcggcg gtgatgaagc cggcaaaggc gcagaagatg ccggagagga tggtgatgac
     6721 aaaggtctgg taaaggtagt gctcgatggt gacacctgac cgggcagaga tcaggtcgtt
     6781 gtggagccag gcgtaggaat cacgccgccc ccggatccag ctcgataccg aggccttcgc
     6841 gttcatccga tcagcttccg gagatccccg aggttctcca tgacctcgtc cggcttgatg
     6901 gcataggact ggaagacctg ggaagcggag tggtaatcct tcatgccctg ctcccgcatg
     6961 gcaccgagga tcttcccccg cctgaggatc tcctgctcga gaacctcggt gctccagccc
     7021 cgggaggcca tgatctggtt gtacacctgg gaccggcccg agtaggtgaa ggtgtcggtg
     7081 accgggtcat aggagaagac ggtgttcacc tggatgttct gggtggcctg gtcgaggccc
     7141 atgatctcca cgatctcctg gcaccgcctc acccgctcgg tcccccggta gatgagggcc
     7201 tggatggaga cgatgtccag ggcctggagc atgttccggg gtaccgcgag gggttcattc
     7261 tccagccggt ggatggcggc atccacgtca gcggcgtgga gggtcgagaa ggtggtgtgg
     7321 ccggtgttca tggcctggaa gagggtctgg gcctcgggtc cccgcacctc gcccacgatg
     7381 atgaactcgg gccgctgccg catggccgcc ttgaggaggt cgaacatgga aatcatgccg
     7441 gatgatccgt cgatcacggc gtcccgggtg acggaggcga tccagttctc ccgctccagg
     7501 gtgatctccc gggtatcctc gatggagacc accttcgcga ggggcgggat gaaatgggag
     7561 acggcattga gcgacgtcgt cttccccgag gcggtcccgc cgatgaagag gaggctcttg
     7621 ttgttctcga cggcaagcca gaagtagacc atggcgtcca cggggaaggt cccgtgctcg
     7681 aggagctcga tgggggtgaa gggctcctcc cggaacttcc ggatggagaa ggaggtcccc
     7741 cggctcgtca ccaccttgga gaaggtgagc tggagacggg aaccgtccgg aagggttgcg
     7801 tccaggatgg gggtaccgat ggagatgtgc ttccccgacc gctgggcgag ggtgatggca
     7861 agggagttca ggtgatcctc gtcaaagacg atgttcgtct tcacgttgtg gaacctccgg
     7921 tggtagagga agatgggaat cttcaccccg tcgcaggaga tatcctcgat ctggggatcc
     7981 ttcatgaggg cgtcgatccg ggaccacccg aggaagttcc ggacgagaaa gtactggagc
     8041 cggaagaggg aggggagatc caccttcacc ccgtactccc ccaggagctc ctccacgagg
     8101 gagaggagga tctgcctctt atcaccccgg atctcctcct cgttcaggag gaggacgtcc
     8161 cggaggtcct cgtacagccg ctccaggagc tcgtactcga aggaggagag ggggggctcg
     8221 aagaggatgt actcgttctg gtggttcttc tggttcaggg cgatgcacac gagggagagg
     8281 ccgcgctcga cccagtacac gtccaccacc tcgaagcccg gccccaggtc gggggtgacg
     8341 agaatgccgt gcttgtccgg gtcatagacc ttctcgctct cgcgccaggt cccgaggcgc
     8401 ctgccaaaga gatggtacgc atccttgagc ctcaggcgcc tcaatacctc aggaagctgc
     8461 atgccggatc ccagaacggg gggagatcca tcctctcccc ccgagaccaa tagacatttt
     8521 gataaaacgc tataaatagt ttcctctttg aaattgcttg gatcatcgac cctgatcaca
     8581 ttttagcgca atcttttaat gctttgcggg aaaacaattc tctatctatg ggagtacagg
     8641 tggcggggag gatgcccacg ggcatcagct ccctggatcc ggtgctggac ggcgggatcc
     8701 cgccgggatc cgtcgttctc ctcgaggggg agatcggggc gggcagccag gagttcgtgt
     8761 acagctccct gatctacctc tccatcctgc gtcagcggca ggacgcgggg cccggcgcga
     8821 gggtcccgga caccatcagc tacatcacct tcacccgcct gaaacaggat gtcctctccg
     8881 agatcgggct ctcctttgcc cccaccctga cccagcacct ggacgggtcg gtcaacttct
     8941 cggacctctc cacccattac ttcgactcga gcgtcgttcc ctccgactgg tactgcaccg
     9001 atggaagcga cgagtgtgaa aagaagcgga aacagagccc ggagaccatg ttctccgagc
     9061 tctcgaccca tgtctcgccc ggtcggaaaa acaccctcct caagctcctc tacctgaccc
     9121 tctccctcgc aaatcccaag aaggcgaaga agggggaggg ctcgagcctc atcatcctcg
     9181 actccctcac cgacctcgcc acccagttct ccgagcccga gcgctggagg gagctcaccg
     9241 ccttcctccg gggcctccag cgggtgacca aggtctggaa ctcgaccgtc ttcctcctcc
     9301 tcacgaaggg ggtcctcgag ccctcccgtg agaaggagat cgccgactgc gtggacgcgg
     9361 tggccctctt ccagtgggag gagaccgtgg cccagcggag gcagcggacc atgttcttcc
     9421 agaagttccg gggggtgatg ccccacctgg aggagcggga cctcgtgaag ttcgtggtcc
     9481 gcatctcccc gggaagcggc ttcgaggtct cgaacatccg ggtggtcgtg tgaacagcga
     9541 cgggaggatc tccctcggga tcgaggggct cgacgcgatg ctcggcgggg gcctgatcaa
     9601 gccctccatc tgcgccctca tcgggaccta cggcaccggg aagaccaccc tctccatcca
     9661 gttcatcacc gaggggcttg ccaacaagga gaagtgcatc tacatcagcc tggacgagcg
     9721 ggtggagatg ctcaagcaga tcatgaagtc acggggcagg gacgtgggct cctatgtgaa
     9781 caagtccctc ttcctcatca agctcgatcc cacgaacttc aacctcatga tcaacagcat
     9841 caaggaggag ctcttcgagc tcatcgagaa gaccggcgcg acccgggtgg tcatcgaccc
     9901 catctccctg ttcgagggga ccttccagag ctccgtggat cggcgcctgg agctcatccg
     9961 gttcatcgag atgatgcgtg accaaccctg caccctcctc atgacctcgg agaccgagtc
    10021 gaacaatccc ctcatgagcc ggtacggcct catcgagtac ctggtcgaca cggtcatcct
    10081 cctccggtac taccggccga gcgacctcac ggccgtccac ctcgctgtcg aggtggtgaa
    10141 gatgcgccag tcccgccact cccgcgagat caagcccttc gagatcatgc aggaccagat
    10201 cctcgtctac tcgggcgcga acgtgttcta attttttttc cccgattgtc cgttacctcc
    10261 gttcggaact ataagcgggt ggaggacact gaagacacca ggacgctctc gggggcttcg
    10321 cccccgccgc tctggcaccc cctattgcga taaccccctc caaggtggtt atcggggatt
    10381 tggaagtact cattatcgca aacatatatt tccgacctgc cagcccatcg taattcgggg
    10441 gatgccgtag tggcgggggc gagcaaaagc gaactcgcga cttcgtcgct cgaggtcgat
    10501 ttctaacgaa acctccctgg tcgcacccct cctgatggcg ggttgctcca gggcgggttc
    10561 tgatgaaccc tcccttcccc caggagcgtt caggcgttga ctgtcgggtc tatcgtttcc
    10621 cgtggtcacc cgataacctg gagtccccgg aaacggacac acgggagaat ggagttcccg
    10681 acgatccggg agcccggggc gatcccgccg atctcccgga gggcatcgaa gaagtttccc
    10741 gagagcatgg ccttcctcac cggctcctgg aatcccccgt cctcgaccca gatggggttc
    10801 tggcattcca ccgagaagtc cccgctcacc gggtttgccg tgtgggctcc caccacgtcg
    10861 tgaacgaaca gggcccgctc catggcgatc tcctccctct gcccgtccag aaccaggttg
    10921 tggaccccga ttgacggtcc gccgccgggc ccgccccggg tcgcggaggc cgtcggtttt
    10981 gcgccgtacc ggtacgcggt cttcaggtcg taggcaaagg aggtgaggac ccccgccttc
    11041 acgaagtcga gccgggcggc ggggagaccc tcggcgtccc aggccgtcgc ccccatcccc
    11101 cgggcaaagg gatcgtcata caggtcgatc cgtgggtcca tgcacctctc cccgaggagg
    11161 gacgcgagcc gcgagcgccc cgcatgcacg ttccggcccg aaagggccgg gacgacgatg
    11221 tacccgagga gctgggcgag ggcgatgggg gagaggagaa catcgcaccg gcaggtttcc
    11281 accggtatcc ccttcgagga atgccatgcg aaaaaggcgg cccgttctcc cacccaccgg
    11341 ggatcgatct ccaggaacgg ggagtgggcg aactcggatc ccgtcgattg cccacagatg
    11401 gtctccaggc tgcagctcac ctcggtcctt tcggccgtat actgtatccc gtgggagttg
    11461 gcgatggtgg cctccgatct ggagagggac cccgatccgc tcgtgatctt cgattccgga
    11521 tgcttcagcg ccccttccag cattccctg
//
LOCUS       DATGDI010000028         4127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_31788, whole genome shotgun sequence.
ACCESSION   DATGDI010000028 DATGDI010000000
VERSION     DATGDI010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31788"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1573
                     /locus_tag="VKO45_01155"
     CDS             <1..1573
                     /locus_tag="VKO45_01155"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HMA04513.1"
                     /translation="LSHPRRYPTGMSGGEILAALRPLAEEKRTFLASELPDLVARKIR
                     GRKPCLRLEPALSFLALGCEKKGDDLIFSRLPGGKTFVPAPDILAEDDRYLRHPSIPG
                     RLQELVEDHIRKKTGKAWDDPVVLERIRGAIRAQKGEYWNQARGRTISYRAGYRILAY
                     LAYQAPASFIQFQHILHDLAADGLLKDRMRVLDIGSGPGTVPLAVISTWSRLSPGDVR
                     IFALEREEEQLEAYRSLVPGFASPDPSVHVEEPVQGDLKSCDPALLPREVDLLVFGSV
                     LNELRELSVGERAALAERISGVLAEGGTLVITEPADLENSLALRRLSAALTRRGLTLY
                     APCTPLWSTSCTPDRCWTFGEGPSFVPPRLMTRLAASSDGYRYLNTDVKFSYALHRRD
                     RLTREAYRIPRGMKALRLSKLQGHLKRRVNVVIAKMSGDLGGRGDHVFKVCDGTPQKP
                     VFAVVPRHHAAHARALLEGRYGEILSLESTLVRFNPARDAFNLFVDRSTKVDFLRHGQ
                     TGRNRGGKGGPKTSP"
     gene            complement(1602..2705)
                     /locus_tag="VKO45_01160"
     CDS             complement(1602..2705)
                     /locus_tag="VKO45_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMA04514.1"
                     /translation="METDTGNGRLAIYLGIFGVMALSNAIVPVLPAFAGGPALQSSIY
                     SAYFLGAFLTVLPAGLLSDRVGRVPVIRAGLAVTVASGLLILVLSDPLLVLAARVAEG
                     VGAGLFVASAMSWMNSREDHERISGDFMASLNLGLVVGLLGSGLIDDALASERAGILA
                     FTLLSVLPAVLVFLIRRDKPGKQVGEGKPRRSRGVIWLYLSAVILVGSTGVVTALYPS
                     YTGRGPASLGIILATMNLATILAVILASRLHLPPVRTIQVAGLVLAGAVLSIPFTPAG
                     FALVGILLGFIMIAQLAFLADSGLPQGEAMGFYNAASYAGMTLLPFVSGVIAEQWGFL
                     PAFALVAFFVVIIAAAVGWPDSREAGAGKVSEP"
     gene            3382..>4127
                     /gene="lsrF"
                     /locus_tag="VKO45_01165"
     CDS             3382..>4127
                     /gene="lsrF"
                     /locus_tag="VKO45_01165"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HMA04515.1"
                     /translation="MVLAYLDIGIQSRMNRIINPKDNRVVMLAIDHPYFQGPTTGLRN
                     MSKTIKTLLPYCDCLMTTRGAVRSNIAPEDVGSKPIMLRATGGNSVLNEELSDEKITV
                     TIEEALRMDAAGVAVSVFIGSANQQQTILNLTDTINHAEEYGIPVLAVTAVGKNMARD
                     LRYLALASRICQDAGARIIKTYYCEEFSRLVDAVSPTAVVVAGGKYSSPPDALQMAYN
                     SVQAGAVGVDFGRNIFQDANPVGMIRAIRS"
BASE COUNT          896 a         1210 c         1267 g          754 t
ORIGIN      
        1 tttatctcat ccacgacggt accctaccgg gatgagcggg ggggagatcc ttgcggccct
       61 gcggcccctg gctgaagaga agcgaacttt tctcgcctca gagctccccg atctcgtggc
      121 caggaagata cgggggagaa aaccctgcct gaggctggag ccggccctct ccttcctcgc
      181 cctcggctgc gagaagaagg gggatgacct catcttctcc cggctgcccg gtgggaaaac
      241 cttcgtaccg gcccccgata tcctcgcgga ggacgacagg tacctccggc acccctccat
      301 ccccgggcgg ctccaggaac tcgtcgagga ccacatcagg aagaagaccg ggaaagcctg
      361 ggatgacccg gtggtcctgg agcggatccg gggggccatc cgggcccaga agggtgagta
      421 ctggaaccag gcgcggggga gaaccatctc ataccgggcc ggctaccgga tccttgccta
      481 cctcgcatac caggcccctg cctcgttcat ccagttccag cacatcctcc atgacctggc
      541 cgccgatggt ctcctgaagg accggatgcg ggtcctggac atcgggtcag ggccgggcac
      601 ggtccctctc gccgtgataa gcacatggag ccggctctcc ccgggggatg tccgcatctt
      661 tgccctcgag cgggaggagg agcagctcga ggcctaccgg tccctcgtcc ccggctttgc
      721 ctccccggac ccctcggttc acgtcgagga gcctgtccag ggtgacctca aatcctgcga
      781 tccggccctc cttccccggg aggtggatct cctcgtcttc gggagcgtcc tcaacgagct
      841 ccgggaactt tccgtcggtg aaagggcggc gctcgcggag aggatctcgg gagtcctcgc
      901 ggagggcggt actctcgtca tcacggagcc ggccgacctg gagaactctc tcgccctccg
      961 gagactctcc gccgcgctca cccggagggg gctcaccctt tacgctccct gtactcctct
     1021 ctggtccacg tcctgcacgc ccgaccggtg ctggaccttt ggggaggggc cgtccttcgt
     1081 cccgccccgg ctcatgaccc gcctcgcagc atccagcgac gggtaccggt acctgaacac
     1141 cgatgtcaag ttctcgtatg cacttcaccg gagggatcgc ctgacccgcg aggcgtaccg
     1201 tatcccccga gggatgaagg ccctccggct ctcgaagctc cagggacacc tgaagcggcg
     1261 ggtgaacgtc gtcatcgcaa agatgtcggg agacctgggg ggccgcgggg atcatgtgtt
     1321 caaggtctgc gacgggaccc cccagaaacc cgtcttcgcc gtggttcccc gccaccacgc
     1381 ggcccatgcc agggcgctcc tggaagggcg gtacggggag atcctctccc tcgagagcac
     1441 tctcgtcagg ttcaacccgg cccgggacgc cttcaacctc ttcgtggacc ggtcaacgaa
     1501 agtggatttc ctgcgccacg ggcaaacggg gaggaatagg ggagggaaag gcgggccgaa
     1561 aacgtctccc tgacgcacca tcggacccgg gatccggctg atcagggttc cgaaaccttt
     1621 ccagccccgg cttcccggga atcaggccat ccgacggcgg cggcaatgat caccacaaag
     1681 aaggcgacca gggcgaaggc aggaagaaag ccccactgtt cggcgataac cccggagacg
     1741 aaggggagga gggtcatccc ggcgtaggag gccgcgttat agaaccccat cgcctccccc
     1801 tggggaagcc ccgagtccgc gaggaaggcg agctgggcga tcatgatgaa gccgaggagg
     1861 atgccgacca gggcaaagcc cgccggcgta aaggggatgg aaaggaccgc ccctgcgagg
     1921 acgaggcccg ccacctggat ggtccggacc ggcgggaggt gaagcctcga tgccaggatg
     1981 accgcgagga tggtcgcgag gttcatggtg gcgaggatga tcccgaggga ggcgggaccc
     2041 ctgccggtgt aggaggggta gagggcagtg accaccccgg tggatccgac gaggatcacc
     2101 gcggagaggt agagccagat gaccccccgg gacctccggg gtttcccctc ccccacctgc
     2161 ttccccggtt tatcccttct gatgaggaag acgaggaccg ccggaaggac cgagaggagg
     2221 gtgaaggcca ggatcccggc ccgctcgctc gccagcgcgt catcgatcag gccggatccc
     2281 aggaggccga ccacgaggcc caggttcagg gaggccatga agtcccccga gatgcgctcg
     2341 tggtcctcgc gggagttcat ccaggacatg gccgaggcca cgaagaggcc cgccccgacg
     2401 ccctcggcaa cccgggccgc aaggacgagg aggggatcgc tcaggacgag aatcaggagg
     2461 ccgctcgcca cggtgacggc aaggccggcc cggatgaccg ggaccctccc gacccggtca
     2521 gagaggagcc cggccgggag cacggtgagg aaggctccaa gaaaataggc agagtagatg
     2581 gaggactgga gggcggggcc cccggcaaag gcggggagga ccgggacgat ggcgttcgag
     2641 agggccatca ccccgaaaat gccgagatag atggcaaggc gaccgttacc ggtatcagtc
     2701 tccattttcg tgctccttga gtcaactatc acatacctcg atgttctcga agatgaattg
     2761 attaggtaac gaatggaagg gcaggattct cacctcgaaa atgaaggcct ggtgcaataa
     2821 gtatccgtct tcatcgaaca gcagatacag aggaaaattt atcctgattt ggtgtgaaca
     2881 ttatagcggt accatgaaat gcgatggatg taaccagaaa gtaacgaaga aagggaagaa
     2941 agacaagtgc aaccagttcc gtactgaatg cgaaaacgtg cagaattgta cgcgttggca
     3001 tcaataagta caccattttt tccatcaatc gtcttatcat cctggagaaa gattttcaca
     3061 attggtcatg ggtattccgt aagccttttt cattgtatga ctaagaatcc attatgcggt
     3121 cgatagatgg gtatatcgat tataagcgtc gggaatattg ccaagatgtt caatgtccgg
     3181 tgcaactgct tttgaataag gaggtggaaa aatctccgaa atatgaagaa atccgggcga
     3241 tctgtgcatc ccgttgtatc cacagcaccc atgagtttca cctctggtta acagggaagg
     3301 gatacctgat cgtgagaccg aagcaggaat agtcccacct ttttttatag ttgtgtagag
     3361 taattctcgt gaagtgtccc catggtactt gcatacctcg acatcggaat tcaaagccgg
     3421 atgaaccgga tcatcaatcc gaaagataac cgggtggtta tgctcgcgat agaccacccc
     3481 tattttcagg ggccgacaac gggcctgcgt aacatgagta aaaccatcaa aacactgctt
     3541 ccctattgcg attgcctgat gacgacacgc ggggcagtgc ggagcaatat tgcgcccgag
     3601 gatgtcgggt caaaacccat catgctgcgt gctacggggg gaaacagcgt tttaaatgaa
     3661 gagttaagtg atgaaaagat tactgttact atagaagaag cccttcggat ggatgcggcc
     3721 ggtgttgctg tcagcgtgtt tatcggctct gccaaccagc aacagacgat tctcaatctc
     3781 accgacacga tcaatcacgc cgaagaatat ggaattcccg ttcttgccgt cacggccgtg
     3841 gggaaaaaca tggcccggga tttacgctat ttagcacttg catcccgtat ctgccaggat
     3901 gccggggccc ggattatcaa aacctattat tgtgaggaat tttccagact ggttgacgcc
     3961 gtttctccca ccgcggtcgt cgttgcagga ggcaagtaca gcagtcctcc tgacgctctc
     4021 cagatggcct acaactctgt ccaggcgggt gccgtgggag ttgatttcgg gcggaatatt
     4081 ttccaggatg ccaatcctgt cggcatgatc agggcaatcc gttccat
//
LOCUS       DATGDI010000029         3990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_2795, whole genome shotgun sequence.
ACCESSION   DATGDI010000029 DATGDI010000000
VERSION     DATGDI010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2795"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..815
                     /locus_tag="VKO45_01170"
     CDS             300..815
                     /locus_tag="VKO45_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04516.1"
                     /translation="MKIDYIIYRDCKCNQRRETMSKWNKRIAKYAGQKVYCNEFDTDG
                     LETDYYIINADGTLSFGNEIRSIDAKFCAPQKMEWWSNRKKHTFLIAAEKNAAKTGIT
                     AEKSVNQFSKEKEILTLEKQIDANYHWCKNGKSINIAANSIAAALQSAELLWSTPSWN
                     LTLYKNGAAIC"
     gene            1205..1468
                     /locus_tag="VKO45_01175"
     CDS             1205..1468
                     /locus_tag="VKO45_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04517.1"
                     /translation="MRIRDYQVTVKFLRRSGPLKTIHSPGIVPKPAESGLISKNNMGN
                     IAEIKKIDDEGNFQVRYEGETEFRPVILDKEIAAILIYGILIK"
     gene            1982..2323
                     /locus_tag="VKO45_01180"
     CDS             1982..2323
                     /locus_tag="VKO45_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04518.1"
                     /translation="MVTATIPMTGKQIDVWYDIEQNDWCYKGEKTPVARFDLLAINLG
                     NEIKAAKQIRANLNEQSTPETAEIARRVSIAITRMEEASMWLGSVCITCPNTVLNENG
                     CAGTLLPEKYF"
     gene            2336..2638
                     /locus_tag="VKO45_01185"
     CDS             2336..2638
                     /locus_tag="VKO45_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04519.1"
                     /translation="MENFIGKYVVCRFKNAGVHAGILKAKEGQTVHLQNSRRLWYWDG
                     AASLSELAMFGVSKPENCKFPCVVNDEIIEEEIEIIPCTEIAQKSISEVPEWKMRK"
     gene            2620..2862
                     /locus_tag="VKO45_01190"
     CDS             2620..2862
                     /locus_tag="VKO45_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04520.1"
                     /translation="MEDAKIGSSGSGDGSGSGSGSGSGSGSGYGYGDGYGDGCGSGSG
                     SGSGSGYGDGYGDGCGYGDGSGSGYGDGYGDGCGDG"
     gene            3032..3556
                     /locus_tag="VKO45_01195"
     CDS             3032..3556
                     /locus_tag="VKO45_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04521.1"
                     /translation="MITINNLNITSKCWKWIGNLTKDFYVDVCYNHDKKDIMFFAWYD
                     FPMLESIIYQAIDKINLSKNWTISQNDEIIKVQDAIWGGRTNTINIGNIKYDYTTARI
                     ATLQWIFQQENHIPDVTKKVEYTTEDLQCCGNCAGGECPDITLQPDHGGYNSNRACPK
                     WEFDGLKQEDRKVE"
     gene            3778..3984
                     /locus_tag="VKO45_01200"
     CDS             3778..3984
                     /locus_tag="VKO45_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04522.1"
                     /translation="MSKNRITRIEAKRDMQARKGISVPECWLCGSLFNLKKYFGFWEC
                     EDCHNDPYNEYEDVDEWEDVIKIL"
BASE COUNT         1353 a          714 c          920 g         1003 t
ORIGIN      
        1 tattgtatgc ataaaaaaat atttgtcgaa aactattgac tcaaaaaacg aatcatgcta
       61 tattgcacct ggaggatata gatgattaat gacgatggat tgactacaat acggatacct
      121 aggcgtctac tgcgcgacct agaggctgga tatctagcac aaactataat atcaacaaaa
      181 agcgagtttt accaatcctg tctcaaggcc ggattgcaag ttttatgcca accaggtaaa
      241 aaacctaaaa gaaataaaaa ataattgtgt ataaatattt tattttcgtt gacataaaaa
      301 tgaaaataga ctatattata tacagagatt gcaaatgtaa tcagaggaga gagacgatga
      361 gtaaatggaa caaaagaata gcaaaatatg caggacaaaa agtttattgt aatgaattcg
      421 atactgacgg tcttgaaaca gattactaca taattaatgc tgacggaaca ttatcatttg
      481 gaaatgaaat taggagcata gacgcaaaat tttgtgcacc acagaaaatg gaatggtggt
      541 caaatagaaa aaaacacaca tttctaattg ccgcagaaaa aaatgcagcg aaaactggta
      601 ttaccgctga gaaatctgtt aatcaatttt ccaaagagaa agaaattcta accctggaaa
      661 aacagattga tgccaattac cattggtgca aaaatggaaa atccatcaat atagcggcta
      721 actcaattgc agctgcattg caatccgcgg aattactttg gagtacccca tcctggaatc
      781 taaccctgta caaaaacggg gcagcaatat gttagacccc agcggcaatt acccagacgg
      841 ggtagatgga tctgaatttg acgaggggtc ggatgaggat caaactgcat acgaaatgga
      901 aatcgagcgc atattggatg ccatagaaga gaacgattat agctagatcg tttctctcgc
      961 cgggtggttt tggatacttt ttccgcccgg ccttctaacc gcgccgccta ttataggtgc
     1021 cctactccca agcataggga taatcaaagc gggaggggaa ggagagataa aatgattact
     1081 attacagcat gtacaaaaca tgacaatacc agatcgacta acattagaaa aattatcggg
     1141 gaaacaaaag acgaaatgat tgaccgggca gtaaaaaaac tgtacggaaa aagatgtttc
     1201 ttttttgagg attcgggact accaggttac ggtcaaattt ttgaggcgat caggcccact
     1261 aaaaacaatt cacagcccgg gaatagttcc aaaaccggca gagtcaggat tgatatcgaa
     1321 gaataatatg ggcaatattg cagagatcaa aaaaatcgat gatgaaggta attttcaggt
     1381 gagatatgag ggtgaaacag aattccggcc tgtaatcctg gataaggaaa ttgcagcaat
     1441 tctaatttac ggaattctga ttaaataaat ttttgaaagg agaatcaaaa gcagagtcaa
     1501 tcattttttt ttaacgccgc aagaaggttg tctggaaaat gacacgcccg gcttgcaacc
     1561 gcggagagct gttttattcc tacagtgagc ggcgttattt ttgatcctga atagctcgcc
     1621 ggtccaactc cggcacagga taacttcccg gttcgcatat tggatagtat gcgactaaaa
     1681 cggtgccaat tgagatgggg gcgcgtggaa ggaaagtcca gtaacgaaac cgcctttcgg
     1741 tatcagggta accggcgtaa gcggataatg tacaatatcg cgctttatcc cgctcgatgg
     1801 gtcaaactgt tgggcgggta gattcacggg cgctatataa atcataaccg gctgaaccat
     1861 acgagaccgc cagggatgct aaacagcaac gtgtattatc acgtataacg tccgcgtttg
     1921 ctccctggta ttttggaaat taggcgtatg cctgaaaata tttttatgga ggtaacaatg
     1981 tatggttact gcaacaatcc ctatgacggg aaaacagatt gatgtctggt atgacatcga
     2041 gcaaaatgat tggtgttata aaggtgaaaa aacaccggtt gcaagatttg atttgctcgc
     2101 gattaatctt ggaaatgaga ttaaagctgc aaaacaaatc agggcaaatc taaacgagca
     2161 atcaacacct gaaaccgccg aaattgctag acgtgtatcg attgcgatca cgcgcatgga
     2221 agaggcgtca atgtggttag gttctgtatg tattacatgc ccaaatactg tactcaatga
     2281 aaatgggtgt gctggtacac ttttaccaga aaaatatttt tgaaaaggag ttttaatgga
     2341 gaattttatt ggaaaatatg tagtctgccg atttaaaaat gccggagtgc atgctggaat
     2401 tttaaaagca aaagaagggc aaacagtaca tctacaaaat tcccgaaggc tttggtattg
     2461 ggatggtgcg gcatcattat cggaattagc aatgtttggt gtatcaaaac cagaaaactg
     2521 taaattccct tgtgtggtga atgatgaaat aatcgaagag gagatagaga taattccgtg
     2581 taccgaaatt gctcaaaaat caatatccga ggtgccagaa tggaagatgc gaaaataggc
     2641 tcctccggct ccggcgacgg ctccggctcc ggctccggct ccggctccgg ctccggctcc
     2701 ggctacggct acggcgacgg ctacggcgac ggctgcggct ccggctccgg ctccggctcc
     2761 ggctccggct acggcgacgg ctacggcgac ggctgcggct acggcgacgg ctccggctcc
     2821 ggctacggcg acggctacgg cgacggctgc ggcgacggat aaaaaatctt aaagcctggc
     2881 accggcaacc agtacaaccg gcaagcccct gggatacaac taatacccaa tcagtcccag
     2941 gggaattttt tagaggatgg caagccttta tctcggcatg tacaggcgct tatagtcgcc
     3001 ttacatgcag tattcgaatt ggaggcaaaa aatgataaca atcaataatc ttaacataac
     3061 atctaaatgt tggaaatgga ttggaaacct aacaaaagat ttttatgtag atgtttgtta
     3121 taatcatgat aaaaaagaca taatgttttt tgcatggtat gattttccaa tgttagaatc
     3181 tataatctat caagcaatag acaagataaa cctttccaaa aattggacaa tatcacaaaa
     3241 tgatgaaatt ataaaagtcc aggatgcgat ctggggcggt agaacaaata ctattaatat
     3301 tggtaatatt aaatatgatt atacgacagc tcgtattgca acattacaat ggatattcca
     3361 acaagaaaac catattcccg atgtcacgaa aaaggtcgag tatacaacag aagatttaca
     3421 gtgctgtggt aattgtgccg gaggagaatg tccagatatc acattgcagc cagatcacgg
     3481 aggatataat tctaatcgtg cctgtcctaa atgggaattt gatggattaa aacaagagga
     3541 tagaaaagtt gaataaacaa gacccgaaac aattattgtg ttataaatgc gctggaagga
     3601 atggtgcatt ccgatggttt ggtggtaatt gggattattc tagttattta gcaatggaaa
     3661 aaatgaggaa tagaaaaaag ttttatgtcg ttcccacccc atacgaaaaa agtatactcg
     3721 catttgccga aagccggaat attttgaaag tagcattttt taaaagggga ttagacgatg
     3781 agtaaaaata gaataaccag aattgaggca aagcgagaca tgcaggcaag aaaaggaatt
     3841 tccgtccctg aatgttggtt atgtggaagt ctttttaatt taaaaaaata ttttggtttt
     3901 tgggaatgtg aagattgcca taatgatcct tataatgaat atgaagacgt tgatgaatgg
     3961 gaagatgtaa taaaaatatt ataagagagg
//
LOCUS       DATGDI010000030         9794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_235502, whole genome shotgun sequence.
ACCESSION   DATGDI010000030 DATGDI010000000
VERSION     DATGDI010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235502"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VKO45_01205"
     CDS             <1..1007
                     /locus_tag="VKO45_01205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA04523.1"
                     /translation="KADLIALGARGRTLRSLVLGSVSASVLRDARVAILLVHDRSIAG
                     GVAMEKYCRSSFARVLIPVDFSKPSLDTVRDLPRLARPGGEVTLLHVLPADLHGWDIP
                     IARRNAEKRLGELEAALGKEGFRARTFTRAGNPAVEILRASEDDDCTLLVLSRFGKKD
                     YVQAVGIGSTAGEVARRAQVPVLVHYPLLHYEVVTRELAPAEFPIADEIWLHYHQQKT
                     DPTDRLFATFADEVPAGVARCRRHPDGLEVDGVFTLDEFRNRGYARRMVEALVSACGK
                     ETLYMHSTLELVPFYRTFGFEPISEEELPRTIRERFSFALGDMAGAHVQPMRREGTHR
                     "
     gene            complement(1118..2278)
                     /locus_tag="VKO45_01210"
     CDS             complement(1118..2278)
                     /locus_tag="VKO45_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04524.1"
                     /translation="MNRGLSAGGILAVLWILVLLTGCLQGGHSGDMGSPSFTTPEATQ
                     VTVETSPSPVTGGEPLTPVPVPPPASDAVWRSFSFASGAHNYSFRIPVAPAEADDTGT
                     SSCPLLSWRPGDESRLASYYSGIFKDPGEDPLYDSLLGELQRIRRTERLNSDEYLELM
                     VHFVQQIPYDPAAPVCPRRPGRLILDGKGDCDERSLLLLGMLYREGFDAAILIFPAKH
                     HATAGIRIDVATQPSFRVFDLRGRKYVYIETTRPSFIGLYPEEFESEDPVIVPTGTGS
                     TTYRAINDVMHIVSTQRRMEDRMRWINETGTGMLTEIQALEEKLTAGTGYSTQEEFDQ
                     DYVRYNSLVTQFNGYVEDFGRIRDVYLFVLDHQVDRLGVAGRIANSKVENLL"
     gene            2440..3828
                     /locus_tag="VKO45_01215"
     CDS             2440..3828
                     /locus_tag="VKO45_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HMA04525.1"
                     /translation="MAELRRDLTLFDLTNIVVGSIVGADIYVASALTAGLIGPFALMV
                     WIAAAVCATVLALVFAYCSYYLPRVGGPFAYVSEAFDDFYGFIAGWSLWIAEILSLPV
                     FAIAFTQYLGFFIPLTPLLEILVKGLFLTALTLVNIVGVRAAGRVNDLLTFVKLLPLL
                     ALVVVGLGIFALGPGKFVANYTPLAPLGFSNFGTALVLIFWAYVGFELGTFPASEVKD
                     PHRTIPKAIISGMIIVTVFYLATNFVVFGTVPWQALAGTNTPLILVGAALFGAAGAAF
                     MTAGALVSVSGSDESGILGTARLSYAMAIDGLFPKVFSLVHPRYGTPFTALVIEGVIA
                     FFLSLFSGISGLISFSVFNMAFSFLITCLALTVISQGRKDILHGQRLLPWIGIAICIY
                     LLLSTSSFDKLVGGLVILAGVPIYVFFSPKTDIYHLKELFISEEAIFVRRMAKQRRFL
                     ANLLRIIHKIVE"
     gene            complement(3837..4730)
                     /locus_tag="VKO45_01220"
     CDS             complement(3837..4730)
                     /locus_tag="VKO45_01220"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HMA04526.1"
                     /translation="MAENVTYPYPPGEGEVRWVSTEWLAGHLKDPGLQIIDSQPNIHD
                     YIRGHIPGALFVEENHWRISGERPGIFLPPEMAELLLRRLGIRSEDPVVVSSSSGPLS
                     VCTTYIGDGLEQTMLAYTLARYGHRRVYILDGGIDTWQAEGRPLSQIFPKVVESRIRV
                     QLRKDFIVDYEEVKAMKDRPDVMLLDARPSPVYEGQGPWPKPGHIPGAVSLPWKSLMD
                     PKNAKLLKPEDQIRAILAEKKITPDKTIICSCGTGREATNEFCLFKYYLKYPKVKLYE
                     GSFTEWTMYPDNPTVTGKNPR"
     gene            complement(4794..5717)
                     /locus_tag="VKO45_01225"
     CDS             complement(4794..5717)
                     /locus_tag="VKO45_01225"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HMA04527.1"
                     /translation="MKLIQVLGIVLIISLGANIGLAYLALQENRGDLGEMSSRMNQLE
                     SDNARLAMQIAQDNLTINAYQNQLGLYRAQLQDLTGLLNASPSGPVGSAVLQGPAVLQ
                     QIQSGGNNPFSQGQVIQSGVMLNITAEVRPGEGRVLVDTQPLMGVVFQDAANTAVYVA
                     QNRTGVSLAGSDVIFSINAPGQVPAVDGPSAGALMTLLVISAIEKKPLREDVTLTGTI
                     DENGNVGAIGGIPDKARAAKDAGKSLLLLPRANANLVTYTQQTRSYYGFTVVRQIPTT
                     VDAKTYVEKNIGVPVQYVNTIDDVLKAATTG"
     gene            5878..6183
                     /locus_tag="VKO45_01230"
     CDS             5878..6183
                     /locus_tag="VKO45_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HMA04528.1"
                     /translation="MSLLSEFMDFLKEYKVVPLAIAFIMGVAATSVVTALVNDIIMGF
                     IKPLLPSGDFKTAVLNVGPFQFAWGDFLAAVINFIIIAFVIFLVAKYMFKEEKVTKK"
     gene            6349..6756
                     /locus_tag="VKO45_01235"
     CDS             6349..6756
                     /locus_tag="VKO45_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04529.1"
                     /translation="MPEELQEQEELTEDLLSRLADRRETVRETAAEALAVATEDEDWR
                     PDDLILCDGIGKVVDLLGDRNPHIVRSALAILTAVASAGHEEELLSHGVIDGLEPLQA
                     HRDPAIRKMARDALWLLTPDVEERMTPEPLEED"
     gene            complement(6782..7051)
                     /locus_tag="VKO45_01240"
     CDS             complement(6782..7051)
                     /locus_tag="VKO45_01240"
                     /inference="COORDINATES: protein motif:HMM:NF019085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HMA04530.1"
                     /translation="MALIGILVLLLVILAFAAVVYFVIRNIGHLVINSIVGLLTLFLV
                     NYFHVMQYAGKPDLEISWITVIICALAGLPGAILVIILALFGITL"
     gene            complement(7186..7578)
                     /locus_tag="VKO45_01245"
     CDS             complement(7186..7578)
                     /locus_tag="VKO45_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMA04531.1"
                     /translation="MEISPVIEVDDRTFHIQVETSKIPAVVMFYSPTCVHCRAMMPSY
                     IQYAQEYRDKVLFARIDVSVNLWTGERFGVKGTPTFKFFCSGRPVTELVGAVYPAILK
                     RTVEEVLVHGKECISKSTEIDYEISGYG"
     gene            complement(7668..8705)
                     /locus_tag="VKO45_01250"
     CDS             complement(7668..8705)
                     /locus_tag="VKO45_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HMA04532.1"
                     /translation="MDIRKVQITGGASYSISLPKGWARAQGIRKNDPLAILIQEDGTL
                     LITPKVPGVKVPRERNFDVSPSMDPVLLFRLLIAAYIGGYSVIRLTAVHRFPPFVRAV
                     VRDFTRMTIGQEVAGETEASIIIKDLLNPVEMPLDRTISRMAVIVRSMHRDAMVALLT
                     ANGTLAEDVIARDNDVDRLHWLVARQFNLILSDPDMARSMKITPAVASRFYVLSRLIE
                     RIGDHGVSVAENAGRLMERRLEASEKDALSEAMHQALDLFVRSIDAFFTGNLEDSHRI
                     IGMVAPLESRCSEMETLAQHHKGEITTALVSIIGSIRRLGEYAEDICENTMNHLVAEG
                     KLEIGPASHSP"
     gene            8806..>9794
                     /locus_tag="VKO45_01255"
     CDS             8806..>9794
                     /locus_tag="VKO45_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HMA04533.1"
                     /translation="MSEPLKKLGITTVLLAGCLILAIAAAGCTGGSAPQTTPGTAATT
                     GAPIPVTTAPVVRQTTAQGSQTGTVSPAPSGTYAVQQIAGKLLVTGSTTVFPIAQVAA
                     EAYMEKNLKADIQVAGGGSSVGVQAVGEGTADIGMSSRDLSPGEKAKYPGLVPVDVAT
                     DAVVVIVNPSNPVSALTLDQVRGLYNGTYQGWGAVGGTLTYPVIIGRDSASGTRVYFN
                     DAVMHGDRYRPDMREMNSNGAVAQAVAQTPAAVGYAGLGFVTSGVKAVAIYSNGRPVV
                     PDAATVLTREYPISRPLYMITRGPPSGLAKDYIDFILSPDGQKIVEEQGFVPLS"
BASE COUNT         1716 a         3098 c         3056 g         1924 t
ORIGIN      
        1 agaaggccga cctcatcgcc ctcggggcac gggggaggac tctccggagc ctggtcctcg
       61 gctcagtctc cgcatcggtc ctccgcgatg cccgggtggc aatcctcctc gtccacgacc
      121 ggtccatcgc cggcggggta gccatggaga agtactgccg gtcttccttc gcccgggtcc
      181 tcatccccgt ggacttctcg aagccctccc tggacacggt gagggacctg ccccgcctcg
      241 cccgtcccgg cggggaggtc acccttctcc acgttctccc ggccgatctc catggctggg
      301 atatccccat cgcccggagg aacgccgaaa aacgcctggg ggagctggag gcggctctcg
      361 ggaaggaggg attccgggca cggaccttca cccgggcagg gaatcccgcg gtggagatcc
      421 tccgggcctc cgaggacgac gactgcaccc tcctcgtcct ctcccgtttc gggaagaagg
      481 actatgtcca ggccgtcggg atcgggagca cggcggggga ggtggcgcgg cgggcccagg
      541 tcccggtcct cgtgcactac ccgctcctcc actacgaggt ggtgacccgg gaactcgccc
      601 ccgccgagtt ccccatcgcc gatgagatct ggctccacta ccaccagcag aagaccgatc
      661 ccaccgaccg gctcttcgcc accttcgccg acgaggtgcc cgccggtgtg gcccggtgcc
      721 gccggcaccc ggacggcctc gaggtggacg gggtcttcac cctcgatgag ttccggaacc
      781 gggggtacgc ccggcgcatg gtggaggccc ttgtttcggc ctgcgggaag gagaccctgt
      841 acatgcattc caccctcgag ctggtcccct tctaccgtac cttcggcttc gagcccatct
      901 ccgaggagga gctcccccgc accatccggg agaggttcag tttcgccctc ggtgacatgg
      961 cgggggcgca tgtgcagccg atgagaaggg aggggaccca tcggtagcgg gtcggacgct
     1021 cacgacccgt ggtgacagca ccgggtacca gcggttgcat cggcgggtta gaccttcgcc
     1081 gggtccttcc acgagatgcc ccgggtggag gagggaatta taacaggttc tccaccttgc
     1141 tgttcgcgat tctcccggcc acccccagcc tgtcgacctg gtgatcgaga acgaaaaggt
     1201 acacatcccg gatcctcccg aaatcctcga catagccgtt gaactgggtc accagggagt
     1261 tgtaccggac gtaatcctgg tcgaactcct cctgcgtcga gtacccggtc ccggccgtca
     1321 gcttctcctc gagagcctgg atctccgtga gcatacccgt cccggtctcg ttgatccagc
     1381 gcatcctatc ctccatcctc ctctgggtcg agacgatgtg catcacgtcg ttgatggccc
     1441 ggtaggtggt ggaacccgtc cctgtcggga cgatgaccgg gtcctcgctc tcgaactcct
     1501 ccgggtagag gccgataaag gaggggcggg tggtctcgat gtacacgtac ttcctgcccc
     1561 ggaggtcgaa gacccggaag gagggctgcg tggcgacgtc gatccggatc ccggcggtcg
     1621 cgtgatgctt cgcggggaag atgaggatgg ccgcgtcgaa tccctcccgg tagagcatcc
     1681 cgaggaggag aaggctcctc tcgtcgcagt caccctttcc gtccaggatc agccggccgg
     1741 gacgccgggg gcagaccggt gccgcgggat cgtaggggat ctgctggacg aagtggacca
     1801 tgagctcgag gtactcgtcg ctgttcagcc tctccgtcct ccggatccgc tggagctccc
     1861 cgaggagaga gtcgtagagg gggtcctctc cggggtcctt gaagatgccc gagtaatagg
     1921 atgcaagccg cgattcatcc cccggccgcc aggagagcag ggggcatgag gatgtgcccg
     1981 tgtcatcggc ctccgccggg gcaacgggaa tccggaagga ataattgtgc gctcccgacg
     2041 cgaagctgaa ggatctccac accgcatcgg atgccggtgg cggcaccggg accggcgtga
     2101 gaggttcccc gcctgtcacc ggagaggggg aggtctccac ggtcacctgg gtggcctccg
     2161 gggttgtgaa cgacggggaa cccatatccc ccgaatgtcc gccctggagg cagccggtga
     2221 ggaggacaag gatccagagg actgccagga tgccccctgc agataatccc cggttcatcc
     2281 tactcccagg tgcccgtcga agcggtctcc tggagcggtt ccgggccggc acatgatcaa
     2341 attctccgtc tctggcatga aagggatttg ggtcagagct gcaggatcca cccgcccacc
     2401 catatcctca tgagggggtg cgcccataga actcccgata tggcggagct ccggcgggac
     2461 ctcaccctct ttgacctgac gaatatcgtc gtcgggtcca tcgtcggggc cgacatctac
     2521 gtcgcctcgg ccctcacggc ggggctcatc ggtcccttcg ccctcatggt ctggatcgcg
     2581 gccgcggtct gcgcgaccgt cctcgccctc gtctttgcct attgcagcta ctacctcccc
     2641 cgggtgggag gcccctttgc gtacgtctcg gaggctttcg atgacttcta cggtttcatc
     2701 gcgggctggt ccctctggat cgccgagatc ctctccctcc cggtctttgc catcgccttc
     2761 acccagtacc tggggttctt catccccctc actcccctcc tggagatcct cgtgaagggc
     2821 ctcttcctca ccgccctcac cctggtaaac atcgtcgggg tgcgggccgc ggggagggtg
     2881 aacgatctcc tgacctttgt gaagctcctc cccctcctcg ccctggtggt cgtgggcctc
     2941 gggatcttcg ccctcggccc ggggaagttt gtggcgaatt acacccccct cgcgcccctc
     3001 gggttctcga acttcggcac cgcgctcgtc ctcatcttct gggcctacgt gggcttcgag
     3061 ctggggacgt ttccggcgag cgaggtgaag gacccgcatc gcaccatccc gaaggccatc
     3121 atctcgggga tgatcatcgt cacggtgttc tatctcgcga caaacttcgt ggtcttcggc
     3181 acggtgccct ggcaggccct tgccgggacg aacacccccc tcattctggt gggggccgcg
     3241 ctgttcggtg ccgccggggc ggccttcatg acggcaggtg ctctcgtgtc ggtctctggg
     3301 tcggatgaga gcgggatcct cgggaccgcc cggctctcct atgccatggc catcgacggc
     3361 ctcttcccga aggtcttctc cctcgtccac ccccggtacg ggaccccctt taccgcgctc
     3421 gtcatcgagg gggtcatcgc gttcttcctc tccctgttct ccgggatctc gggcctcatc
     3481 tccttctcgg tcttcaacat ggccttctcc ttcctcatca cctgcctcgc cctcacggtc
     3541 atctcgcagg gcaggaagga tatcctccac ggccagaggc tgctgccatg gatcgggatc
     3601 gccatctgca tctatcttct cctctccacg tcctccttcg acaagctcgt ggggggtctc
     3661 gtcatcctcg cgggggtccc catctacgtc ttcttctcgc caaagacgga tatctaccac
     3721 ttaaaggagc tcttcatctc agaggaggcc atctttgtcc gtcggatggc gaaacaacgc
     3781 cggttcctcg cgaacctcct caggatcatc cataaaatag tggagtgaag ggccgcctac
     3841 cgcgggttct tcccggtcac cgtggggttg tcggggtaca tggtccactc ggtaaaggac
     3901 ccctcgtaga gcttcacctt cgggtatttc aggtagtact tgaagaggca gaactcgttc
     3961 gtcgcctccc tcccggtccc gcaggagcag atgatggtct tgtccggggt gatcttcttt
     4021 tccgcgagga ttgcccggat ctggtcctcg ggcttcagga gcttcgcgtt cttcgggtcc
     4081 atcaggctct tccaggggag gctcacggcc ccggggatgt ggccgggctt gggccagggt
     4141 ccctggccct cgtacaccgg ggacggccgg gcatcgagga gcatcacgtc gggccggtcc
     4201 ttcatggcct tcacctcctc gtaatcgacg atgaagtcct tcctgagctg gacccggatc
     4261 ctggattcca cgaccttcgg gaagatctgc gagaggggcc tgccctctgc ctgccatgta
     4321 tcgatgccac catcgaggat atacaccctc cggtgcccgt accgggcgag ggtgtaggca
     4381 agcatcgtct gctcgagacc gtccccgata taggtcgtgc agaccgaaag gggccccgag
     4441 ctggaggaca ccaccaccgg gtcctcggac cggatcccga gcctcctcag gaggagctcc
     4501 gccatctcgg ggggaaggaa gatcccgggc ctctccccgg agatcctcca gtggttctcc
     4561 tcgacaaaga gggcacccgg gatgtgcccc cggatgtagt cgtggatgtt cggctgggag
     4621 tcgatgatct ggaggccggg gtccttcagg tggccggcga gccattccgt cgagacccac
     4681 ctcacctcgc cctcgccggg cggataggga tacgtcacat tctctgccat gtagatcctc
     4741 ctcgttccgt gtaggtgatg aggctggggg gttaaaagga tttcgtggat ccttcagccc
     4801 gtggtcgcgg ccttcagcac atcatcgatg gtgttcacat actggaccgg gaccccgatg
     4861 tttttctcga cataggtctt cgcgtccacc gtggtcggga tctggcggac gacggtgaaa
     4921 ccgtaatagg atcgggtctg ctgggtgtac gtgaccaggt tcgcattcgc ccttggaagg
     4981 aggaggagcg acttccccgc atcttttgcc gcccgggcct tgtcggggat ccccccgatg
     5041 gcccccacgt taccgttctc gtcgatggtc ccggtaaggg tcacgtcctc ccggaggggc
     5101 ttcttctcga tggccgagat gacgaggagg gtcatgaggg ccccggcgct cgggccgtcg
     5161 acggccggga cctggccggg cgcgttgatg gagaagatga catcgctccc cgcaagggaa
     5221 acgcccgtcc ggttctgggc gacgtacacc gcggtgttcg ccgcgtcctg gaagacgacc
     5281 cccatgaggg gctgggtgtc gacgaggact cgcccctcac ccggcctcac ctctgcggtg
     5341 atgttgagca tcacccccga ctggatcacc tgtccctggg aaaaggggtt gttcccgccg
     5401 gactggatct gctggaggac cgcaggcccc tggagcaccg ctgatcccac gggcccggaa
     5461 ggggaggcgt tgaggaggcc ggtcaggtcc tggagctgtg cccggtacag gccgagctgg
     5521 ttctggtagg cattgatggt gagattatcc tgggcgatct gcatcgcgag cctcgcgtta
     5581 tcggactcga gctggttcat ccggctcgac atctcgccga gatccccccg gttttcctgg
     5641 agggcaaggt aggcaaggcc gatgtttgcc ccgagggaga tgatgaggac gatcccgaga
     5701 acctggatca gtttcatgga agccgggtag accgattgat tatatacgct ttccctcacc
     5761 tcccaaaatg tgaggacggg cagagatcgg ccggaatata cggttattca gcggtcccgg
     5821 ttcaattcgg tatgaataaa tacggccgcg ggaattcttt cccgaggtgg tttacacatg
     5881 agtcttttaa gcgaattcat ggatttcctc aaggagtaca aggtcgtccc cctcgccatt
     5941 gccttcatca tgggcgttgc ggcgacttcc gtggtgactg cccttgtgaa cgacattatc
     6001 atgggtttca tcaagcccct gctccccagc ggagacttta aaaccgcggt cctcaacgtc
     6061 ggacccttcc agttcgcatg gggagacttc cttgctgccg tgatcaactt catcatcatc
     6121 gccttcgtca tattccttgt cgcaaagtac atgttcaagg aagagaaggt cacaaagaag
     6181 taggccccag gaatcacccc ctctttttca ggtgtcccgg cccctcgcag cggggatatg
     6241 cctcaccgaa cgggaacagc cgtcaggctt ttaaatggcc ggcaggcttt taaaagccat
     6301 aaggctttta agccatcccg tccatcgact ggaagaggaa aaaagaagat gcccgaggaa
     6361 ctgcaggagc aggaggagct gaccgaggat ctcctctccc ggctcgcgga tcgcagggag
     6421 accgtgcggg agacggcggc ggaggccctc gccgtggcaa ccgaggacga ggactggagg
     6481 ccggacgacc tgatcctctg cgacgggatc gggaaggtgg tcgaccttct cggcgacagg
     6541 aacccccata tcgtccggtc ggcgctcgcc attctcaccg ccgtggcgtc ggcgggtcac
     6601 gaggaggagc tgctcagcca cggggtgatc gacggtctgg agccgttgca ggcccaccgg
     6661 gatccggcga tccggaagat ggccagggat gcgctctggc tcctcacgcc ggatgtcgag
     6721 gaaagaatga cgccggagcc cctggaagag gactaaaaac agaagggtgt tcgtcccggg
     6781 ttcagagagt aatcccgaac agggcgagga tgatcacgag aattgctccg gggagccctg
     6841 cgagggcgca gatgatgacc gtgatccagc tgatctcaag gtcgggcttc cccgcgtact
     6901 gcatgacatg gaagtagttc acgagaaaaa gggtgagaag cccgacgatg gagttgatca
     6961 ccaggtggcc gatgttccgg atcacgaagt acacgaccgc cgcaaaggcg aggatgacga
     7021 gaaggaggac gaggatgccg atcagtgcca tgtacaggca tcagtctccc gcggtaaaga
     7081 gggtatcgct ccacaccctt ccgcccagaa aaagccccat tcggcccttt ttcgttcttc
     7141 aggcccttct gggagggaat ttaccggggg taaaaaagcc cttcttcacc cgtacccgct
     7201 gatctcgtaa tcgatctcgg tggatttgct gatgcactcc ttcccgtgga cgagaacctc
     7261 ctccacggta cgtttcagga tcgccggata gaccgccccc acgagctcgg tgacggggcg
     7321 gccgctgcag aagaacttga aggtgggtgt tcccttcacc ccgaaccgct cccctgtcca
     7381 gaggttcacc gacacgtcga tccgggcaaa gaggacttta tccctgtact cctgggcata
     7441 ctggatatag gagggcatca tggcccggca gtggacgcag gtcggggagt aaaacatcac
     7501 caccgcgggg atcttcgatg tctccacctg gatgtggaag gtgcggtcgt cgacctcgat
     7561 gacaggggaa atctccatgc actgccccgg aattacacct tgaacgggga atcatgttaa
     7621 aggttgtgga gacgggtagg gagatacagg cgggaatatc ctgactttca gggggagtgg
     7681 gaagcagggc ctatctccag cttcccttcg gcgaccaggt ggttcatggt attttcacag
     7741 atatcctccg catattcccc gagtctccgg atggaaccga tgatggagac aagagcggtg
     7801 gtgatctccc ccttgtgatg ctgggcgagc gtctccattt ctgagcaccg ggactcaagc
     7861 ggggccacca tcccgatgat ccggtgggag tcctccagat tcccggtgaa gaaggcgtca
     7921 atgctccgga cgaagagatc cagggcctgg tgcatggcct ccgagagggc atccttttcg
     7981 gatgcctcga gcctccgctc catgagcctg cctgcattct ccgcgaccga gaccccgtga
     8041 tctccgatcc gctcgatgag gcgggagagg acatagaacc gggatgccac ggcgggagtg
     8101 attttcatgg atcgggccat gtccggatca gagagtatca ggttgaactg gcgggcgaca
     8161 agccagtgaa gccggtccac atcattgtcc cgggcgatga catcctctgc aagagtgccg
     8221 tttgcggtga ggagcgccac catggcatcc cggtgcatgg accggacgat gaccgccatg
     8281 cggctgatgg tccggtcaag aggcatctcc acggggttga ggaggtcctt gatgatgatg
     8341 gaggcctcgg tttccccggc cacctcctgg ccgatggtca tccgggtgaa atccctgacg
     8401 acggcccgga cgaaaggggg gaaccggtga accgcggtga ggcggatgac agagtacccc
     8461 ccgatatagg cggcgatgag gagccgaaag aggagtaccg gatccatgga tggggagaca
     8521 tcgaagtttc gctcccgcgg gaccttcacc ccgggtacct tcggggtgat gaggagggtc
     8581 ccgtcttcct ggatgaggat cgcgaggggg tcgttcttcc ggattccctg ggccctcgcc
     8641 catcccttcg gcagggagat gctgtaggag gccccgccgg tgatctgaac cttccggatg
     8701 tccatcggtt ttctccctat atctctatct ttaattccta tatatataga gatatagtgt
     8761 ttatttcggg tatcactatc tatcctaccg ccgacctcct gatcggtgtc ggaacccttg
     8821 aagaaactcg gtatcaccac cgtcctcctc gccgggtgcc tcatcctggc catcgcagcc
     8881 gccggttgca ccggtggcag cgccccgcag acgaccccgg ggaccgcggc caccaccggt
     8941 gccccgatcc cggtcacaac tgctcccgtt gtccggcaga ccaccgcaca aggctctcag
     9001 accggaaccg tcagcccggc gccgtccggg acctatgcgg ttcagcagat cgccggtaag
     9061 ctgctggtta ccggttcgac cacggtcttc cccatcgccc aggttgccgc cgaggcgtac
     9121 atggagaaaa acctgaaggc cgatatccag gtggccggag ggggttcgag tgtcggggta
     9181 caggccgtcg gcgaagggac cgccgacatc ggcatgtcct cccgggacct cagccctggc
     9241 gagaaggcga agtacccagg cctcgtcccc gtcgatgtcg cgaccgacgc ggtcgtggtc
     9301 attgtcaacc cctccaaccc cgtatctgcc ctcaccctgg accaggtccg gggcctctac
     9361 aacggcacct accagggctg gggcgctgtc ggcgggaccc tgacttaccc ggtgatcatc
     9421 gggcgtgaca gcgcatccgg gacccgggtg tacttcaatg atgccgtgat gcacggcgac
     9481 aggtaccggc ccgatatgag ggagatgaac tcgaacgggg ccgttgcgca agccgtggcc
     9541 cagacccctg cggccgtcgg ttatgcgggc ctcggctttg tcacgtcggg tgtaaaggcc
     9601 gtggccatct actcgaacgg gcggcctgtg gtgcccgacg ccgcaaccgt cctcacccgc
     9661 gagtacccga tctcccgccc cctgtacatg atcacgcggg gcccgccgtc agggctcgcg
     9721 aaggactaca tcgatttcat cctctcgcct gacgggcaga agattgtcga ggagcagggc
     9781 ttcgtgcccc tctc
//
LOCUS       DATGDI010000031         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_119337, whole genome shotgun sequence.
ACCESSION   DATGDI010000031 DATGDI010000000
VERSION     DATGDI010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119337"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..514)
                     /locus_tag="VKO45_01260"
     CDS             complement(47..514)
                     /locus_tag="VKO45_01260"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HMA04534.1"
                     /translation="MTGKKGETGTRRTVPEKGPGGADAEKGPGQMTGEQRIIALGACP
                     FAGLLGMEILEARPGWARVAMDTTGKENFLGVAHGGAIFALADQAFAAAANRGDLLQV
                     AVSVHIHYLSPAKGRLEAVARLIAETDTYSHFLVEVKSGERLVATFEGVAYKA"
     gene            complement(530..1231)
                     /locus_tag="VKO45_01265"
     CDS             complement(530..1231)
                     /locus_tag="VKO45_01265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMA04535.1"
                     /translation="MISVVVPTYNEEENIGGCLESLNRQTLPRSEYETIVVDGNSKDR
                     TREIAAPLADLVFIQTSPRVGGARNDGALKARGEIVAFADADCILPPDWLQRIRDDFA
                     RHHPVEVYGTVYPREGGLKNRIALGMANTFARIGYHTRTLYYSLGCNTAVNRDVFIRA
                     GMYRTMDAGEDIEISLRMKKFGRVLFDPKVRVGFSMRRYQQFGTVKSIYQWLYIVARG
                     GETSKYSYSRRDYGK"
     gene            complement(1228..2205)
                     /locus_tag="VKO45_01270"
     CDS             complement(1228..2205)
                     /locus_tag="VKO45_01270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMA04536.1"
                     /translation="MRINFVVEDFSVFKYIGSATAARSVYRNMPRDADLDLTWNSARR
                     GVDLAHFHTFGPFALAYRTYAKGVKVLTAHSTPHTNVGNIAESGLINRLYPPIYRGFD
                     HIVTVSDPCDREIRAMLPGMETTMIPNGVDRRIFQRYDDKRRAFRAALGADEDETVVL
                     ALGQQTPRKGIYDFLELAARTPETIWVWVGGFPYGRLSKDFARIEREKRKASENVVFT
                     GFVPDVTEAYSGADLFFFPSKAETFGLVILEALASGLPVAARGIPELREIFGDSVAFF
                     DDLDQAQDLVQSKDRLRLSGEKAREFTGRFDIRIIAEAHAKLYRRLAEP"
     gene            complement(2202..>2905)
                     /locus_tag="VKO45_01275"
     CDS             complement(2202..>2905)
                     /locus_tag="VKO45_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMA04537.1"
                     /translation="TGGIEALVQAIARPIDEFITMDSPVTSVTRNGEGFLVRTGGREM
                     KADRVVSTIPLPSLLPCLTDVPPRVMEACRDLRSTALLSVFIGLRGEVPPYSWVYVPS
                     PEIGLFNRVSFPSNYSPAVTPPGHSSILAEITCARDDPLFSTPDEGIVSHVLGGLARM
                     GVLSDPGRVVFTAVERSPFAYVVYDLGYMEKIGAVRGFCEGRGIHLVGRFSRFEYLNM
                     DGVIRGAMDFVGGIA"
BASE COUNT          487 a          971 c          942 g          505 t
ORIGIN      
        1 cggcagggtg gcctcgggat tgagataatc ccctgcctcc cgcgggttac gccttgtacg
       61 cgacaccctc gaacgtcgcg acgaggcgtt cgccgctctt cacctcgacg aggaagtggg
      121 agtacgtgtc ggtctcggcg atgagccggg cgacggcctc gagcctcccc ttcgccggcg
      181 agaggtagtg gatgtgcacc gagaccgcga cctggaggag gtctccccgg ttcgcggcgg
      241 cggcaaaggc ctggtccgcg agggcgaaga tggctccccc gtgggcgacc ccgaggaagt
      301 tctccttccc ggtggtgtcc atcgcgaccc tcgcccaccc gggccgggcc tcgaggatct
      361 ccatcccgag aagccccgca aagggacagg ccccgagggc gatgatgcgc tgttcgccgg
      421 tcatctgccc gggccccttt tccgcatccg cccccccggg ccctttttcc ggaaccgtcc
      481 tcctggttcc cgtttctccc ttctttcccg tcatggagac ctccccggcc tacttcccgt
      541 aatcccgccg ggaatacgag tacttcgagg tctccccgcc cctggccacg atgtagagcc
      601 actggtagat gctcttcacg gtaccgaact gctggtaccg ccgcatggag aagcccaccc
      661 tcaccttcgg atcgaagagg accctcccga acttcttcat ccggagggag atctcgatat
      721 cctcgccggc atccatggtg cggtacatcc ctgcccggat gaacacgtcc cggttcacgg
      781 ctgtgttgca tcccagggag tagtacaggg tcctcgtgtg gtaaccgatc cgggcaaagg
      841 tgttggccat gccgagggcg atacggttct tgagtccgcc ctcccggggg tagaccgtgc
      901 cgtacacctc gacaggatga tgcctcgcga agtcatcccg gatccgctgc agccagtccg
      961 gcgggagaat gcagtcggca tccgcgaagg ccacgatctc tcccctcgcc ttcagggccc
     1021 cgtcgttcct cgccccgccg acccgggggc tcgtctggat gaagaccaga tcggcgaggg
     1081 gggccgcgat ctcccgggtc cggtccttcg aattcccgtc caccacgatg gtctcgtatt
     1141 ccgaccgggg gagggtctgc cggttgaggg actcgaggca acccccgatg ttctcctcct
     1201 cattgtacgt gggtaccacg acggagatca cggctctgcc agcctccggt acagcttcgc
     1261 gtgggcctcg gcaataattc ttatgtcaaa ccggccggtg aactccctcg ccttttctcc
     1321 cgagagccgc aaccggtcct tcgactgcac gagatcctgt gcttggtcca ggtcgtcaaa
     1381 gaaggccacc gagtccccga agatctccct gagctcgggg attccccggg ccgccacggg
     1441 caggccgctt gcgagggcct cgaggatcac gaggccgaag gtctcggcct tcgaggggaa
     1501 gaagaagagg tccgccccgc tgtacgcctc ggtgacatcc gggacgaatc cggtgaagac
     1561 cacgttctcc gaggccttcc tcttctcccg ttcgatccgg gcaaagtcct tcgagagccg
     1621 gccgtagggg aacccgccga cccacaccca gatggtctcc ggcgtgcggg cggcgagttc
     1681 caggaagtcg tagatcccct tccggggggt ctgctgcccg agagcgagga ccacggtctc
     1741 gtcctcgtct gccccgaggg cggcccggaa ggcccggcgc ttgtcgtcgt acctctggaa
     1801 gatccggcgg tcgaccccgt tcgggatcat ggtggtctcc atcccgggga gcatcgcccg
     1861 gatctcccgg tcgcagggat ccgagacggt gacgatgtgg tcgaagcccc ggtagatggg
     1921 cgggtagagc cggttaatga ggccgctctc ggcgatgttc cccacgttcg tgtggggcgt
     1981 ggagtgggcg gtgaggactt tcaccccctt tgcataggtg cggtacgcga gggcgaaggg
     2041 cccgaaggtg tggaagtggg cgaggtccac gcccctcctc gccgagttcc aggtgagatc
     2101 caggtccgca tccctcggca tgttccggta cacggatcgg gccgcggtcg cgctcccgat
     2161 gtacttgaag accgagaagt cctcgacgac gaagttgatc ctcacgcgat tccccccaca
     2221 aagtccatcg cacccctgat aaccccgtcc atgttcaggt actcgaaccg ggaaaaccgg
     2281 cccacgagat ggatccccct cccctcgcag aagccccgga ccgccccgat cttttccatg
     2341 tacccgaggt cgtacaccac gtaggcgaag gggctccgct ccacggcggt gaagaccacc
     2401 cggcccgggt cggaaaggac acccatcctc gcgaggcccc cgaggacatg ggacacgatc
     2461 ccctcgtccg gcgtggagaa gagggggtca tccctcgcgc aggtgatctc ggcgaggatg
     2521 gaggagtggc cgggcggggt gacggcgggg ctgtagttcg acgggaagga gacccggttg
     2581 aagaggccga tctcaggcga ggggacgtag acccacgagt acgggggaac ctccccccgg
     2641 agcccgatga agaccgagag gagcgcggtg gacctgaggt cacggcatgc ctccatcacc
     2701 ctcgggggaa catccgtgag acacgggagg agggagggga gggggatggt tgagacgacc
     2761 cggtcggcct tcatctcccg ccctcctgta cggacgagga atccctcccc gtttcgggtg
     2821 acggaggtga caggcgaatc catggtgatg aactcatcga tgggccgggc aatggcctgc
     2881 acgagggcct cgatcccccc ggtcc
//
LOCUS       DATGDI010000032         1669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_119388, whole genome shotgun sequence.
ACCESSION   DATGDI010000032 DATGDI010000000
VERSION     DATGDI010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119388"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1557)
                     /locus_tag="VKO45_01280"
     CDS             complement(<1..1557)
                     /locus_tag="VKO45_01280"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HMA04538.1"
                     /translation="MLTPPDYTGLPKEEREDGQIEAIKGTFVNVEAQSTKGVREGKLF
                     LNQESQVILDIKGKQLRGSLLVLHPGSYSIRVKDEFGFENPNPVAYPIRLIPDKYPEG
                     EILSPAQDLEISGTEVISIVYAARDDFGVTSVSLGYQVGGKERRINLKSPGGSRSVEP
                     ETFKWDLASLDLMPGDRVIYRLEIEDNDSVSGPKVAYSRSFTLSMRDERSRAVKEGED
                     AQQIADALLDLLADQLEETQAREPLAQRMEEILNRVDRNLKETDKADRFDLEALRRNL
                     ASLKERMPKESKETVTQEMERLALLAEDISKKARMKEVEALAREIRNRQRRLLDAMQE
                     FKGPLTKEAQEAFMKELRKLEELLHSVMDALSKMASRLPEEFINNQDLSGLEFQDMFK
                     DLEEIQKKLMAGDLAGALEAAQRLLQALSEMMAALSRAGAQAGMSPMDRLQGEMSRQS
                     GELDKILAEQKEILKETEKIEGALRQRVEEETEKRLNQSLPELEELLKRLNRSLSAEQ
                     KELVEGLEELL"
BASE COUNT          299 a          543 c          443 g          384 t
ORIGIN      
        1 gagaagttcc tccaatccct cgaccagctc cttttgctcg gcggagaggg agcggttgag
       61 acgtttcaaa agctcctcca gctccggaag agactggttc aaccttttct ccgtctcctc
      121 ctcgacccgt tgcctcaacg ccccctcaat cttctccgtc tccttcaaga tctctttctg
      181 ctcggccagg atcttgtcca gttcacccga ctgacgggac atctcgccct gcaacctgtc
      241 catgggggac atccccgcct gggcccccgc ccggctcaga gcggccatca tctccgacag
      301 ggcctgcagg agcctttgcg cggcctcgag ggccccggca aggtctcccg ccatcagctt
      361 tttctggatc tcttcgaggt ccttgaacat atcctggaac tcgagaccac tgagatcctg
      421 gttgttgatg aactcctccg gcagccggct cgccatcttg ctcagggcat ccatgacgga
      481 gtggaggagc tcttccagct ttctcaactc cttcatgaaa gcctcctggg cctccttggt
      541 gaggggcccc ttgaattcct gcatggcgtc gagaagacgc ctctgacgat ttcgtatctc
      601 ccgggcgagg gcttcgacct ctttcattct cgcttttttc gagatgtcct cggcaaggag
      661 ggcgagcctc tccatctcct gcgtcacggt ctccttcgac tccttcggca tcctctcctt
      721 gagggaggca aggttgcgcc ggagcgcctc caggtcgaaa cggtccgcct tgtcggtctc
      781 tttgagattc cggtcgaccc ggttcaggat ctcctccatc ctctgcgcca gcggttcccg
      841 tgcctgggtc tcttctaact gatccgccag aaggtctaac agggcatccg caatctgctg
      901 ggcatcttcc ccttccttga cagcccggct cctctcgtct cgcatggaca gggtgaagga
      961 gcgggagtaa gccaccttcg ggccggagac cgaatcgttg tcttcgatct ccaggcgata
     1021 gatgacgcga tcaccgggca tcaggtcaag actcgcaagg tcccatttaa aggtctcagg
     1081 ctctacggaa cgagaaccac caggactctt caggttgatg cggcgctcct tccctccaac
     1141 ttgatatccg aggctcaccg acgtcactcc aaaatcgtcc ctggcggcgt agacgatgga
     1201 aataacctcg gtgcccgaaa tctccaggtc ctgcgcaggg ctgaggatct cgccttcggg
     1261 atacttgtcc gggatcagac ggatgggata ggcgacagga ttggggttct caaagccaaa
     1321 ctcgtccttg acccggatcg aataggaacc gggatgaaga acaagcaggc ttcccctgag
     1381 ttgcttccct tttatgtcga gaatgacctg gctctcttga ttgagaaaaa gcttcccttc
     1441 cctcaccccc ttcgtgctct gcgcctccac gttcacgaag gtccccttga tggcctcgat
     1501 ctgcccgtcc tccctctcct ctttgggaag ccccgtgtag tcgggggggg tcagcatcac
     1561 cctgacgctc ccgatctcag gagggtccac cacgcgcagg gaatactccg gagaggctgc
     1621 acggccgttg taggcctgat aacggaagga gaactgggct gacgcaatt
//
LOCUS       DATGDI010000033         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_322990, whole genome shotgun sequence.
ACCESSION   DATGDI010000033 DATGDI010000000
VERSION     DATGDI010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322990"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VKO45_01285"
     CDS             <1..791
                     /locus_tag="VKO45_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA04539.1"
                     /translation="DKVIVIDQSPIGRTPRSNPATYTKVFDDIRRVFAETKEAKVRGY
                     SPGRFSFNLRGGRCEACQGEGLIRIEMNFLPDVYIECEECKGTRYNRETLEVKYRGRS
                     IADVLAMSVEEATALFEKIPSIRQKLETLSRVGLDYVKLGQSSTTLSGGEAQRIKLTR
                     ELAKRATGKTLYLLDEPTTGLHFHDTKKLIQVLDDLVGRGNTAVIIEHNLDVIKSADH
                     IIDLGPEGGDLGGEVVAAGTPEEVSRVPASYTGQFLGRILNGHG"
     gene            784..>2216
                     /gene="uvrC"
                     /locus_tag="VKO45_01290"
     CDS             784..>2216
                     /gene="uvrC"
                     /locus_tag="VKO45_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HMA04540.1"
                     /translation="MADPGQIPSSPGCYLFRDEKGTILYIGKAKDLKKRVSSYFQRSS
                     PDSRIRAMVAKVDSVDFMVTDNEVEALVLENNLIKRHQPRYNIDLRDAKTYAYIMLTK
                     EEFPRILMARRPSGDGSYFGPFVSARERDHVLRAVKRAFGLRTCRRLPKRACLRHHMG
                     SCSAPCVAGITPEEYGLRVKRVESILRGGSAELLTDLRREMADRSREQDFEGAMAIRD
                     QIGAVEHLATRQKVERRRDHDEDIINYLETEEKVYLMLFSVERGTLAEKEEFIFDASP
                     DFMEEFLVQYYSDNPPPKELILPNAVSLPVVNFLALRRGNRVIVSVPVKGEKMQLLDL
                     VRKNLEQTFFGEKLRLEALGEALHLPGPLSAIECFDISHLSGTSTVGSMVRFVDGRPE
                     KKGYRRFRIRTVGGVDDPAAISEVVRRRYSRVVREGQKLPDLVIIDGGKGQLAAARAA
                     LAGVHVDVPLISIAKREEEVYAPGFSFP"
BASE COUNT          396 a          725 c          741 g          354 t
ORIGIN      
        1 tcgacaaggt catcgtcatc gaccagtccc ctatcggccg gaccccccgg tcgaacccgg
       61 caacgtacac gaaggtcttt gacgacatcc gccgggtctt tgccgagacg aaggaggcga
      121 aggtccgggg gtacagcccg ggccggttct ccttcaacct ccggggcggc cggtgcgagg
      181 cctgccaggg cgaagggctc atccggatcg agatgaactt cctccccgac gtgtacatcg
      241 agtgcgagga gtgcaagggg acccggtaca accgcgagac cctggaggtg aagtaccggg
      301 gcaggtccat cgccgacgtt cttgccatgt ccgtggagga ggccacggcc ctcttcgaga
      361 agatcccctc catcaggcag aagctcgaga ccctctcccg ggtaggcctc gactacgtga
      421 agctcgggca gtcctcgacg accctgtccg gcggcgaggc ccagcggatc aagctcaccc
      481 gggagctcgc gaaaagagcg acgggaaaga ccctctacct cctggacgaa ccgacgaccg
      541 gcctccactt ccatgacacg aagaagctca tccaggtcct ggacgacctg gtgggccggg
      601 ggaataccgc ggtcatcatc gagcacaacc tggatgtcat caagtccgcg gaccacatca
      661 tcgacctcgg gcccgagggg ggcgacctgg gaggggaggt ggtggccgcc ggcacccccg
      721 aggaggtgag ccgggtcccg gcgagctaca cggggcagtt cctcggccgg atcctgaacg
      781 gccatggctg accccgggca gatcccctcc tccccgggct gctacctctt ccgggatgaa
      841 aaggggacca tcctctacat cgggaaggcc aaggacctga agaagagggt gtcctcctac
      901 ttccagagga gttcccccga ctcccggatc cgggcgatgg tcgcgaaggt ggactccgtc
      961 gacttcatgg tgaccgacaa cgaggtcgag gccctcgtcc tcgagaacaa cctcatcaag
     1021 cggcaccagc cccggtacaa catcgacctc cgggacgcga agacctacgc gtacatcatg
     1081 ctcacgaagg aggagttccc ccggatcctc atggcccggc ggccgtcggg ggacgggtcg
     1141 tacttcgggc ccttcgtctc cgcccgggaa cgggaccacg tcctccgggc cgtgaaacgg
     1201 gccttcgggc ttcgcacctg ccggcggctc ccgaagcggg catgtttgcg gcaccatatg
     1261 ggctcctgct ccgcaccgtg cgtcgccggg atcaccccgg aggagtatgg gttgcgggtg
     1321 aagcgggtgg agtcgatcct caggggagga tcggcggagc ttcttaccga cctccgtcgc
     1381 gagatggccg accggtcgag ggagcaggac ttcgagggtg ccatggccat ccgggaccag
     1441 atcggggccg tggagcacct cgccacccgg cagaaggtgg agcgccggcg ggaccatgac
     1501 gaggacatca tcaactacct ggagaccgag gagaaggtgt acctcatgct cttctccgtc
     1561 gagaggggga cgcttgccga gaaggaggag ttcatcttcg atgcctcgcc ggatttcatg
     1621 gaggagtttc tcgtccagta ctactcggac aacccgcccc cgaaggagct catcctcccg
     1681 aacgcggtct ccctcccggt cgtgaacttc ctcgccctcc ggcgggggaa ccgggtcatc
     1741 gtctcggtgc cggtgaaggg ggagaagatg cagctcctgg acctcgtccg gaagaacctg
     1801 gagcagacct tcttcgggga gaagctccgg ctcgaggccc tcggcgaggc cctgcacctg
     1861 ccgggtcccc tctccgccat cgagtgcttc gacatctccc acctctccgg tacctccacc
     1921 gtcggttcga tggtgcggtt cgtggacggc cggccggaga agaaggggta ccgccggttc
     1981 aggatccgga ccgtcggcgg ggtggacgat cccgcggcga tatctgaagt tgtgcggagg
     2041 cgctacagcc gggttgtgcg ggaggggcag aagctccccg acctcgtgat cattgacggc
     2101 gggaaggggc agctcgccgc cgcccgggcg gccctcgccg gggttcacgt ggatgtgccc
     2161 ctcatctcca tcgcgaagag ggaggaggag gtctatgccc cggggttctc cttccc
//
LOCUS       DATGDI010000034         5933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_410572, whole genome shotgun sequence.
ACCESSION   DATGDI010000034 DATGDI010000000
VERSION     DATGDI010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410572"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1417)
                     /locus_tag="VKO45_01295"
     CDS             complement(86..1417)
                     /locus_tag="VKO45_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048063716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HMA04541.1"
                     /translation="MAELRIAVINSVQDEAGRNIRRHLEELLSGRESTGGKAEDGGIT
                     FELHEVEGRLIHADGIDRKFPSDLILFISRHTSREPAPVLTVHVTGNLSQADLGGKPR
                     SLPPACPPWMQAVLRELSEKAPPGYAVSYEVTHHGPTELSTPSFFVEIGSTAAEWADP
                     RAGRAVAESILAAQPGDAVNLLGFGGNHYAARQTEIGCTSRGAFGHIAHSREAPAVDR
                     DMVTLMRDRTRAVAAYLDRKALDPRDVTRIEEILGEIGLPVLSEGEIVSLGEMEWSSW
                     LAARSLAFSVHPGSRLHPHRVSTLGRPVPVSLDPVLVTEAVRADEKALISGLDTLPVV
                     HLSGGGREVLPAFITDEKDRAGVIHGLISLCVKLIIERQNAAVEGDRLIIRRTRFDPG
                     KAHELGVPRGPLFGRLQAGEEISLGGRVITPSMVQTARVTEIHIPGLELYL"
     gene            1467..1643
                     /locus_tag="VKO45_01300"
     CDS             1467..1643
                     /locus_tag="VKO45_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04542.1"
                     /translation="MDEALIKKALEESGIRSDLPCQKAFEISEKYGIPKMDIAKYCNR
                     HKVKIRKCQLGCFG"
     gene            1640..2842
                     /gene="glp"
                     /locus_tag="VKO45_01305"
     CDS             1640..2842
                     /gene="glp"
                     /locus_tag="VKO45_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HMA04543.1"
                     /translation="MSLFLSVVTLEEALGVIRRIAPPPREEEIPLGDCLHRVLARDVG
                     TPVDIPGFSRTTVDGYAVRAADTVGAGEAVPAILRLAGEILMGSRPSLFLRPGEGISA
                     PTGAMLPAGADASVMVEYTERIGDEILVKRPVAPGENVILAGEDFPAGTVILPRGRCL
                     SPRDAGVLAAAGHERVMVYRRPVVGIISTGNELVPVGDVPGPGQVRDANGPMCAAFAG
                     SVGCLPRPYGIARDDRQTLGETLERAVHECDAILLSGGSSKDVRDLAASVIGEKGEVL
                     VHGIGIAPGKPTIIGKARGRPVIGLPGHPASAYIILHALAGPLLSAMSGSPWSERKVP
                     AVLAENIPSQKGRVEFVRVRIEKERAIPCFGKSGLLNTFVGSDGMVMVPADREGFEAG
                     ETVEVILW"
     gene            2836..4695
                     /locus_tag="VKO45_01310"
     CDS             2836..4695
                     /locus_tag="VKO45_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HMA04544.1"
                     /translation="MVKRYLSLTPLGEAREILGRSFVFTPVTEKVPIGAAAGRITASP
                     VVARFSVPGAHLAAMDGIAVLAEETFGASEQRPVTLRGAVRVNTGNLLPPGCDAVIMI
                     EEVRIDGDRFTIRSPVSPWQHVRPVGEDIGETEMILPSRHPVRPHEIGALAAYGITEL
                     EVLTVRIGLIPTGSELVPPGERPGPGQVVESNTIMAAAWLEGLGARCTRLPLTPDEPD
                     LIRSRLSEAAEENDLVIISAGSSAGTRDFTADAIADLGEVLVHGVAMKPGKPVILGRI
                     RGKPVIGLPGYPLSAVTVLRELVTPLLSRYGLPSEQGEEIRATLASTLASELGVEEFV
                     FLSVGRIRDRWVAVPHGRGAGVQMSGVRANAYLSIGAGKEGIEAGEEVVAHLMVPAGE
                     AERALILTGSHDPALDLLADNLARAGVGLHSAHQGSMGGLIALKKRVCHGAPMHLLAD
                     DGSYNIPYLSRHISGENLVLLCLAGREQGIISRDGLSLGDLPGARFINRQKGSGTRIL
                     LDHLLVEKGIPPASIPGYDRELTTHLAVALAVKTGEADAGLGVYSAAKALGLSFVPVA
                     REPYELVFYADTWEDPRVQALAAAVSSPPFREGLEGLGGYDVSLTGRLRRLPP"
     gene            complement(4658..5104)
                     /locus_tag="VKO45_01315"
     CDS             complement(4658..5104)
                     /locus_tag="VKO45_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HMA04545.1"
                     /translation="MQIAVIGKEECTGEEYGAARAAGSRIAEGHAVLICGGRGGVMEG
                     ACRGARESRGVTVGILPDTGKGNPYLDVVIRTDMGYGRNAIVVQSADAVVAIGGGYGT
                     LSEIAIALRAGKRVFGYHTWEIDGVEGCDTPEGAVKGAVAAASRSG"
     gene            5194..>5933
                     /locus_tag="VKO45_01320"
     CDS             5194..>5933
                     /locus_tag="VKO45_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HMA04546.1"
                     /translation="MKQVESTYQAGAVEARVREFWTAGDIYHRVRELRAGGKPFFFVD
                     GPPYTTGNIHLGTAWNKILKDAILRYHRLKGRHIVERAGYDMHGLPIEVKVEHNLGFS
                     TKKEIEGYGIGAFVDECRRFALENKDLMSDQFRALGVWLDFENPYMTVTPGYIEAAWW
                     TLARAEEKGMLERGHRVVNWCPRCETAIADSEVEYEDRKDPSIFVKFPVKGRDREYLV
                     IWTTTPWTLPANVAAAVMPGAIYARVRA"
BASE COUNT          970 a         2024 c         1958 g          981 t
ORIGIN      
        1 atcggcacga cgggcggctg tgccccggcc gccgtggcct tctgttcctc ctctccccgt
       61 gccaacgcct cgtcgatgat ggacttcata ggtacaactc caaccccggg atatgtatct
      121 ctgtaaccct ggctgtctgg accatggacg gggtgatgac cctcccgccc aggctgatct
      181 cctcgcctgc ctgcaacctg ccaaagagag gccccctcgg aaccccgagc tcatgtgcct
      241 tcccggggtc gaaccgggtc ctcctgatga tcaggcgatc accttcaaca gccgcattct
      301 gacgttcaat aatgagtttc acacacaggc ttattaagcc atggatgacg ccggcccggt
      361 ccttctcatc cgtgataaag gccgggagaa cctccctccc gcccccggag aggtggacaa
      421 ccgggagggt atcgagcccg gagatgaggg ccttctcgtc agccctcact gcttcggtga
      481 ccagaaccgg atcgagggag acggggaccg gtctcccgag ggtggagacg cggtggggat
      541 ggaggcggga accgggatgg acggaaaacg cgagggagcg ggccgcaagc caggaggacc
      601 actccatctc cccgagggag acgatctctc cctcggagag aaccgggagg ccgatctcac
      661 cgaggatctc ctcgatacgg gtcacatcgc ggggatcgag agccttccgg tcgaggtacg
      721 cggcgacagc ccgggtccgg tcccgcatca gggtgaccat gtcccggtcg acggccggcg
      781 cctcccggct gtgggcgatg tgcccgaagg caccccggga ggtgcagcct atctccgtct
      841 gccgtgccgc gtaatgattc cccccgaagc cgaggaggtt cacggcgtct cctggctgtg
      901 cagcgaggat gctctccgcg accgccctcc ccgccctcgg atctgcccac tctgcagcag
      961 tcgagcctat ctccacgaag aaggacggcg tcgagagctc cgtcggcccg tggtgggtca
     1021 cctcgtagga gacggcgtac ccggggggag ccttctccga aagctcccgg agaaccgcct
     1081 gcatccacgg cgggcatgcc ggagggagag agcggggttt cccgccgaga tctgcctgcg
     1141 agagattgcc ggtcacgtgg acggtgagga cgggcgccgg ctcccggctc gtgtgccgcg
     1201 agatgaagag gatgagatcc gaggggaact tccggtcgat cccgtcggcg tggatgagcc
     1261 ggccctccac ctcatggagc tcgaaggtga tgcccccgtc ctccgccttt cccccggtgg
     1321 attcccgacc ggagaggagt tcctccaggt gacggcggat gttccttccc gcctcgtcct
     1381 gcaccgagtt gatgacggcg atccggagct ccgccatgtt tcaccaatgg taaacactat
     1441 gtttcgggaa aggaatgatt gttaccatgg acgaagccct tatcaaaaag gcccttgagg
     1501 agtccggaat caggtcagat ctcccctgcc agaaggcctt cgagatctcg gagaaatacg
     1561 ggatccccaa gatggacatc gcgaagtact gcaaccgcca caaggtcaag atccggaagt
     1621 gccagctggg ctgcttcgga tgagcctttt cctttcggtg gtcaccctcg aggaggccct
     1681 gggggtcatc cgccggatcg cgccccctcc ccgggaggag gagatcccac tcggcgattg
     1741 cctccaccgg gtcctcgccc gggacgtggg gaccccggtc gatatccccg ggttctcccg
     1801 cacgaccgtc gatggctacg cggtgcgggc ggcggacacc gtcggggcgg gcgaggcggt
     1861 ccccgccatc ctcaggctgg cgggagaaat tctcatggga agcagaccct ccctcttcct
     1921 ccggccgggc gaggggatct ccgccccgac cggcgccatg ctcccggcgg gggcggacgc
     1981 ttccgtgatg gtcgagtaca ccgagcggat cggggacgag atcctcgtga agaggcccgt
     2041 ggccccgggc gagaacgtca tcctcgccgg ggaggatttc ccggcgggga ctgtcatcct
     2101 cccgcgtgga aggtgcctct cgccccggga tgcaggggtc ctcgccgccg cagggcatga
     2161 acgggtgatg gtgtaccgga ggccggtcgt ggggatcatt tccaccggga acgagctcgt
     2221 ccccgtcggg gatgttcccg gccccggaca ggtgagggat gcaaacggcc ccatgtgcgc
     2281 cgccttcgca ggctccgtcg gatgcctgcc ccgtccctac gggatcgccc gggacgaccg
     2341 gcagaccctc ggtgagaccc tggagagggc ggtgcatgag tgcgacgcca tcctcctctc
     2401 gggaggatcc tccaaggacg tccgggatct ggcggcgtct gtcatcggag agaaggggga
     2461 ggtgctcgtc catggcatcg gcatcgcgcc ggggaagccc accatcatcg ggaaggcccg
     2521 ggggaggccg gtcatcggcc tcccgggcca tccggcctcg gcctacatca tcctccatgc
     2581 cctcgcagga cccctcctct cggcgatgtc cggatccccg tggtcggaga ggaaggtccc
     2641 ggccgtcctc gccgagaaca tcccctccca gaagggcagg gtggagttcg tccgggtcag
     2701 gatcgagaag gaaagggcga tcccctgctt cgggaagagc ggtctcctga ataccttcgt
     2761 gggaagcgac ggcatggtca tggtgcccgc cgaccgggag gggttcgagg ccggtgagac
     2821 cgtcgaggtg atcctgtggt gaagcggtac ctttccctga cccctctcgg ggaagcccgg
     2881 gagatcctcg gccgctcctt tgtcttcacg ccggtcaccg aaaaggttcc catcggtgcg
     2941 gccgcgggga ggatcactgc atccccggtg gtcgcccggt tctcggtccc gggtgcccac
     3001 ctcgcggcca tggacgggat cgccgtcctc gccgaggaga ccttcggcgc gagcgagcag
     3061 cgccctgtca ccctccgggg tgccgtccgg gtgaacaccg gaaacctcct ccctcctggc
     3121 tgtgacgcgg tgatcatgat cgaggaagtg aggattgacg gcgaccggtt cacgatccgg
     3181 agcccggtca gcccctggca gcacgtccgc cccgtcgggg aggacatcgg ggagacggag
     3241 atgatccttc cctcccggca cccggtccgg ccccacgaga tcggggccct cgcggcctac
     3301 ggaatcacgg agctcgaggt tctcacggtg aggatcgggc tcatccccac cgggagcgag
     3361 ctcgtgcccc cgggcgagcg cccgggcccc ggccaggtcg tggagtccaa caccatcatg
     3421 gccgcggcat ggctggaggg tcttggggcc cggtgtaccc gccttcccct cactccggac
     3481 gaaccggatc ttatccgttc ccgcctctcg gaggccgcgg aggagaacga cctcgttatt
     3541 atttccgccg gctcctcggc gggcacgcgg gactttaccg ccgatgccat cgccgacctc
     3601 ggcgaggtcc tcgtccacgg cgtcgccatg aagccgggca agccggtgat cctggggagg
     3661 atccggggaa agccggtcat cggcctcccc ggctaccccc tctcagcggt cacggttctc
     3721 cgggaactcg tcaccccgct cctctcccgg tacgggctcc cttcggagca gggggaggaa
     3781 atccgggcca ccctcgcctc caccctcgcc tccgagctcg gggtcgagga gttcgtcttc
     3841 ctctcggtcg ggaggatccg ggaccggtgg gtggcggtgc cccacgggcg gggtgccggc
     3901 gtgcagatga gcggcgtgcg ggccaacgcg tacctttcca tcggggcagg gaaggagggg
     3961 atcgaggccg gggaggaggt cgtggcccac ctcatggtgc cggccgggga ggccgaacgg
     4021 gccctcatcc tgacggggag ccacgatccg gccctcgacc tcctggccga caacctggcc
     4081 cgggcgggag tcggcctcca cagcgcccac caggggtcca tgggaggcct catcgccctg
     4141 aaaaagaggg tctgccacgg ggcacccatg cacctcctcg ccgatgatgg cagctacaac
     4201 atcccgtacc tcagccgcca tatctccggc gagaacctgg ttctcctctg tctcgcgggg
     4261 agggagcagg gaatcatctc ccgagacggc ctttccctcg gggatctccc gggagcccgg
     4321 ttcatcaacc ggcagaaagg atcgggaacc cggattctcc ttgatcacct cctcgtggaa
     4381 aagggcatcc cgccggcatc cattccgggc tacgaccggg aactgaccac ccatctcgcg
     4441 gtggcgcttg ccgtgaagac cggcgaggcg gatgcgggcc tcggggtgta ctccgcggca
     4501 aaggccctcg gcctctcctt cgtcccggtg gcccgggagc cctacgagct cgtcttttac
     4561 gccgatacct gggaggaccc cagagtgcag gccctcgccg ccgcggtcag ctccccccct
     4621 ttccgggaag gcctcgaggg tctcgggggc tacgatgtca gcctgaccgg gaggctgcgg
     4681 cgactgcccc cttgaccgcc ccttccggcg tgtcgcaccc ttccaccccg tcgatctccc
     4741 aggtgtggta cccgaagacc cttttccccg ccctgagagc gatggcgatc tccgagaggg
     4801 tgccgtagcc tcccccgatg gccaccaccg cgtccgcgga ctggacgacg atggcgttcc
     4861 tcccgtaccc catgtctgtc cggatcacga cgtcgaggta cgggtttccc tttcccgtgt
     4921 cgggaaggat gcccacggtc accccgcggg actcccttgc tccccggcag gcaccctcca
     4981 tcaccccgcc cctgccgccg cagataagga cagcatgccc ttctgcgatg cgggagccgg
     5041 ccgctctcgc agctccgtat tcctcgcccg tgcactcctc tttgccgatg accgcgatct
     5101 gcattcccgg agttttgaac acgtgtggtt taaaaaatgc tttcttatgg aagatccaac
     5161 atggtagaga atgacggcct ctggaggatc cccgtgaagc aggtggaaag cacctaccag
     5221 gccggcgcgg tcgaggcacg ggtccgggag ttctggacgg ccggggacat ctaccaccgg
     5281 gtgcgggagc tccgggccgg cgggaagccc tttttcttcg tcgacgggcc gccctacacc
     5341 acggggaaca tccacctggg cacggcctgg aacaagatcc tcaaggacgc catcctccgc
     5401 taccaccggc tgaaggggag gcacatcgtc gagcgggcgg ggtacgacat gcacggcctc
     5461 cccatcgagg tgaaggtgga gcacaacctg ggtttctcga cgaaaaagga gatcgagggc
     5521 tacggcatcg gggccttcgt cgacgagtgc cggcgctttg ccctcgaaaa caaggacctc
     5581 atgtccgacc agttccgggc cctcggggtc tggctcgact tcgagaaccc ctacatgacc
     5641 gtcaccccgg ggtacatcga ggcggcctgg tggaccctcg cccgggccga ggaaaaaggg
     5701 atgctcgagc ggggccaccg ggtcgtgaac tggtgccccc ggtgcgagac cgccatcgcc
     5761 gactcagagg tggagtacga agaccggaag gacccctcca tcttcgtgaa gttcccggtg
     5821 aagggccggg accgcgagta cctcgtcatc tggaccacca ccccctggac cctcccggcg
     5881 aacgttgccg cggccgtcat gcccggcgcc atctatgccc gggtccgggc cga
//
LOCUS       DATGDI010000035         1689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_119788, whole genome shotgun sequence.
ACCESSION   DATGDI010000035 DATGDI010000000
VERSION     DATGDI010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119788"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..491)
                     /locus_tag="VKO45_01325"
     CDS             complement(183..491)
                     /locus_tag="VKO45_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04547.1"
                     /translation="MTSKFGKGFVVNLILLAKHLGLPPDKAFFGAADHLDEMVLPESF
                     RGTEVEKALEHLRKAVIWHQPGSMDKEDYHEAVLAMHRLAIAVDHELGIRDADVGQYD
                     "
     gene            complement(543..1352)
                     /gene="cbiQ"
                     /locus_tag="VKO45_01330"
     CDS             complement(543..1352)
                     /gene="cbiQ"
                     /locus_tag="VKO45_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HMA04548.1"
                     /translation="MIEDLFTLEMHTYRDSPVHRLDARAKILAVLAVIIGIVAVPYTP
                     AVYAPALLLLLFFALLWGLSGISPLVYARRLALILPVWGAVILVQVFLKNPYYPLFHP
                     VLSLPLGISVYSESVEFATILAVKFLVSVSYIILLSSTTTLQDLLSGAGRLGLPAEFA
                     LVLGMMARYLYVFGYMYRKVVEALHTRCFDPLDGALPYRYRIRQLGYTVGSLFIRSYE
                     QGERVYTSMCCRGYGRHSHLFIEKKPFSGTDQVFLSASLALVAAALLLGFF"
     gene            complement(1349..>1689)
                     /locus_tag="VKO45_01335"
     CDS             complement(1349..>1689)
                     /locus_tag="VKO45_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMA04549.1"
                     /translation="VRDLTGSLNALCRDYGMTVIFSTHDVSLVPELADSIYVMHRGQI
                     VAEGTVGEIFARAEMLRSFRLDVPPIPRLLRSLREHGVEISPAYSYAEAEQSLLSAFG
                     KRSSTGDQNP"
BASE COUNT          343 a          466 c          624 g          256 t
ORIGIN      
        1 cgggaggatg gcccgggagg gcacggccag gtctctcctg cctggccgct ttcgggggag
       61 ggagggttca tcagaacctg acctagagca acccgccatc aggcggggtg cgaccaggga
      121 ggtttctccc gaaattctcc cttcatctcg atcgtccacg tgaacgtggg cgagccctgc
      181 atctagtcgt actggcccac gtcggcgtcc ctgatcccga gctcatggtc caccgcgatg
      241 gcgagccggt gcatcgcgag gaccgcctcg tggtagtcct ccttgtccat ggaccccggc
      301 tggtgccaga tgaccgcctt ccggaggtgc tcaagggcct tctccacctc cgtcccccgg
      361 aaggactcgg ggagtaccat ctcgtccagg tggtccgcgg ccccgaagaa ggccttgtcc
      421 gggggaagcc cgaggtgctt tgcgaggagg atcaggttca ccacgaagcc cttgccgaac
      481 ttgctcgtca tatgcttaag atgaagggag gaggggggag aaatacgtta tgacaggggg
      541 ctctagaaaa acccgaggag gagggcggcc gcgaccaggg cgagggaggc cgagagaaac
      601 acctggtcag ttcccgaaaa gggttttttc tcgatgaaca ggtgggagtg acgaccgtat
      661 ccccggcagc acatggaggt gtagacccgc tcaccctgct cgtaggaccg gatgaagagc
      721 gacccgacgg tataccccag ctgccggatc cggtaacggt agggaagggc gccgtccagg
      781 gggtcgaagc accgggtgtg gagggcctcc accaccttgc ggtacatgta cccaaagaca
      841 tagaggtagc gggccatcat cccgagaacg agggcgaact ctgcggggag gccgagccgg
      901 cccgcccccg aaaggaggtc ctggagggtg gtggtcgagg agaggaggat gatgtacgag
      961 accgagacga ggaactttac cgcgaggatg gtggcgaact ccaccgattc gctgtacacg
     1021 gagatcccga gggggaggga gaggacgggg tggaacaacg ggtaatacgg gtttttcagg
     1081 aagacctgga cgaggatgac ggccccccag acggggagga tgagggcgag ccgcctcgcg
     1141 tacaccaggg gggaaatccc ggagaggccc cagaggaggg caaagaacag gaggaggagg
     1201 agggcggggg cgtagacggc gggggtgtaa gggacggcca cgatcccgat gatgaccgcg
     1261 aggaccgcga ggatcttcgc ccgggcatcg agccggtgga caggactgtc ccggtacgtg
     1321 tgcatctcga gggtgaagag atcctcgatc atgggttttg gtccccggtg gacgatcgct
     1381 tcccgaaggc ggagaggagg gactgctcgg cctccgcata ggagtaggcg ggggagatct
     1441 ccaccccgtg ctcccggagg gatcggagga gcctcgggat gggggggacg tccagacgga
     1501 aggaccggag catctcggcg cgggcgaaga tctccccgac ggttccctct gccacgatct
     1561 gaccccggtg catcacgtag atggaatccg cgagttcggg gacgagggag acgtcgtggg
     1621 tggagaagat gacggtcatc ccgtagtccc ggcagagggc gtttaaggac ccggtcaggt
     1681 cgcgcaccc
//
LOCUS       DATGDI010000036         3297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_410775, whole genome shotgun sequence.
ACCESSION   DATGDI010000036 DATGDI010000000
VERSION     DATGDI010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410775"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2592)
                     /locus_tag="VKO45_01340"
     CDS             complement(<1..2592)
                     /locus_tag="VKO45_01340"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HMA04550.1"
                     /translation="MAPRFHAYPAGQVLAELRSGPGGLSPDEARARLALHGPNRLEEK
                     VKESPLLLFARQFTDLLIVILAIAAAVSGLLGEWLDAAAIIAIIALNGVIGFLQEYRA
                     EKALEALKKMIAPVARVVRGGAEAMIDARDLVPGDVILLAEGDRVPADGRLLETIALE
                     ADEAALTGESVPVPKDAGMTCDPGEPLPCHGNMAFLGTVITRGRGRAVITATGMRTEL
                     GKIAEAVAEEPEEATPLQKKLAYLGRQLSAAALAIVGVIFIVGIARGLPALGMFLVAV
                     SLAVAAIPEGLPAVVTITLSLGVQRMAGKHAIIRRLPAVETLGAATVICTDKTGTLTL
                     NEMTVRRVSVNGTFLRVTGEGYVTGGDFLEEPAGAAVSPAGVPGLVTLLETGVLCNNA
                     ALPSVGSTKIPGDPTEASLLVLAAKAGLDPEQVRAAHPFAREIPFDSARKAMTVVRTT
                     DGHRRAYVKGAPELLVERCTKILWDGSERDLAGPGREAILAANTEMASGALRVLGFAY
                     RDLPGEIPDDEIEKDLVFIGLAGMMDPPRPEAGAAIQACRKAGIRVIMITGDNPLTAG
                     AVARELGLETGEILTGADLERATDAELRDQVEGVGIYARVSPEHKLRIVDALKARGEI
                     VAMTGDGVNDAPALSRADIGVAMGITGTEVAKEASDMVITDDNFASIVLAVEEGRVIY
                     ANILKAVKYLISCNIGELITIFVAIMAGLASPLAPIQILWMNIVTDSPPALALAMNPG
                     DPDVMKRPPQDPGERILGRRNAMGMGAIGLLMAAVTLAVFILYGGTKTALAGTMAFSV
                     IILFQQFYALESSASGERHLHEMGLFRNRWLWAAFLFGIASQVLITAWGPAQAIFETV
                     ALGPAEWG"
     gene            complement(2760..2870)
                     /locus_tag="VKO45_01345"
     tRNA            complement(join(2760..2795,2833..2870))
                     /locus_tag="VKO45_01345"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2834..2836),aa:Met,seq:cat)
     gene            complement(3014..>3297)
                     /locus_tag="VKO45_01350"
     CDS             complement(3014..>3297)
                     /locus_tag="VKO45_01350"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04551.1"
                     /translation="GVFGRSDGLIQVTYFGWPLYYYSGDRSPGDANGYGFNKLWYVMG
                     TGGVVTLAPTTKPPTPVPTTPPVYNYGGGYSGGGGGGGGGGSGGGGGGY"
BASE COUNT          549 a         1101 c         1126 g          521 t
ORIGIN      
        1 cccccactcc gccggcccga gggccaccgt ctcgaagatc gcctgcgccg gcccccaggc
       61 cgtgatgagg acctgggagg cgatcccgaa gaggaaggcc gcccagagcc agcggttccg
      121 gaagagcccc atctcgtgga ggtgcctctc cccggaggcg ctcgactcga gggcatagaa
      181 ctgctggaag aggatgatga ccgagaaggc catggtccct gcgagggccg tctttgtgcc
      241 cccgtacagg atgaagaccg ccagggtgac ggcggccatg aggaggccga tggcccccat
      301 ccccatcgca ttgcggcggc cgaggatccg ttcgccgggg tcctggggcg gccgcttcat
      361 cacatcgggg tcgccggggt tcatggccag ggcgagggcc ggggggctgt ctgtcacgat
      421 gttcatccag aggatctgga tgggggcgag ggggctcgcg aggcccgcca tgatggcgac
      481 gaagatggtg atgagctccc cgatgttgca ggagatgagg tacttcaccg ccttgaggat
      541 gtttgcgtag atcaccctcc cctcctccac ggcaagcacg atggaggcga aattgtcgtc
      601 ggtgatcacc atgtcgctcg cctcctttgc cacctctgtg ccggtgatgc ccatggcaac
      661 gccgatgtcc gcccgcgaaa gcgcgggggc gtcgttcacc ccgtcgccgg tcatggccac
      721 gatctccccc cgggccttca gggcgtccac gatgcggagc ttatgctcgg gcgagacccg
      781 ggcatagatc cccactcctt ccacctggtc ccggagttcc gcgtcagtgg cccgttcgag
      841 atcggcgccg gtcaggatct ccccggtctc caggcccagc tcccgtgcca ccgcccccgc
      901 tgtcaggggg ttgtctccgg tgatcatgat cacccggatc cccgccttcc ggcaggcctg
      961 gatcgccgcc ccggcctcgg gcctcggggg gtccatcatg ccggcgaggc cgatgaagac
     1021 gaggtccttc tcgatctcat cgtcgggaat ttcaccgggg agatcccggt acgcgaaccc
     1081 gaggacccgg agggcccccg aggccatctc cgtgtttgca gcaaggatcg cctcccgccc
     1141 gggcccggcg agatcccgtt ccgagccgtc ccacaggatc ttcgtacacc tctcgacgag
     1201 gagctccggc gcacccttca cgtaggcccg ccggtggcca tccgtggtgc ggaccacggt
     1261 catcgccttc cgggcggagt cgaaggggat ctcccgggcg aaggggtggg ccgcccggac
     1321 ctgttccgga tcgagccctg ccttcgccgc gaggacgagg agggaggcct cggtgggatc
     1381 tcccgggatc ttcgtgctcc ccaccgaagg gagggccgcg ttgttgcaga gcaccccggt
     1441 ttcgaggagc gtcacgagac cggggacccc cgcgggggaa actgcggccc cggccggctc
     1501 ttcgaggaag tcccccccgg tcacgtagcc ctcacccgtg acccggagga aggtcccgtt
     1561 cacggagacc ctccgcacgg tcatctcgtt cagggtgagg gtgcccgtct tatcggtgca
     1621 gatgacggtc gccgcgccca gggtttccac cgcggggagc cggcggatga tggcatgctt
     1681 cccggccatc cgctggaccc ccagggagag ggtgatggtg accacggcgg gaagcccctc
     1741 ggggatggcc gcgaccgcga gggagaccgc gacgaggaac atcccgaggg ccgggagccc
     1801 ccgggcgatg cccacgatga agatcacccc cacgatggcg agggccgcgg ccgagagctg
     1861 cctccccagg taggcgagct ttttctggag gggggtcgcc tcctccggtt cctctgccac
     1921 cgcctcggcg atcttcccga gctcggttct catcccggtc gcggtaatca ccgcccttcc
     1981 cctcccgcgg gtgatgaccg tccccaggaa ggccatgttc ccgtggcagg ggagcggctc
     2041 ccccgggtcg caggtcatcc cggcgtcctt gggtaccggg accgattccc cggtgagggc
     2101 ggcctcgtcg gcctcgaggg cgatcgtctc gaggaggcgc ccgtcggcgg gaacccggtc
     2161 gccctcggcg aggagaatga catcgccggg gaccaggtcc cgggcatcga tcatcgcctc
     2221 ggccccgccc cggacgaccc gggccaccgg ggcgatcatc ttcttcaatg cctcgagagc
     2281 cttctccgcc cggtactcct ggagaaagcc gatgaccccg ttcagggcaa tgatggcgat
     2341 gattgccgcg gcgtccagcc actccccgag gaggccggac acggcagccg cgatggcgag
     2401 gatcacgatg aggaggtcgg tgaactgccg ggcgaagagg aggagggggc tctccttcac
     2461 cttctcctcc agccggttcg gcccgtggag ggcgaggcgg gcccgtgcct catcgggtga
     2521 gagccctccc ggcccggacc ggagctccgc gaggacctgg cccgccgggt acgcgtggaa
     2581 ccggggtgcc acaggggatt catcggggga agacatggaa tggctccgtc gatactcctc
     2641 cggggatccg ggttcccggg cggataccgc gatgagaaat cggtggcatc agccttaaaa
     2701 ttgcggggtc cggcagggag aaagatagag acgcccggaa cggggtgagt cagaggtgat
     2761 gctgcgggcg gggatcgagc ccgcgatctc cagatatccc agatgatggc gctccgacgt
     2821 tcgcgaaaac cctatgagtc tggcgcccta accggctagg ccaccgcagc atatagaaga
     2881 ggaaatctgt tttgataaag gttctcatcg cgatgcatgg gatctatcgt cgggcgggaa
     2941 aggagcgaac ctcgaaagag ttggggatgg gatgaaaaat ggcaggagtg tccgggaaaa
     3001 agcggcgaag ggatcagtac cctccgccgc caccaccgga acctcccccg ccgcctccac
     3061 cgccaccacc actgtaaccg ccgccgtaat tatagaccgg tggcgtggtc gggaccgggg
     3121 tcgggggctt cgtggtcggg gcgagggtca cgacaccccc ggtgcccatc acgtaccaga
     3181 gcttattaaa gccgtacccg ttggcatccc ccgggctcct gtcacccgag tagtaataca
     3241 ggggccatcc gaagtaggtg acctgtatca gcccgtcaga tcggccaaac acaccaa
//
LOCUS       DATGDI010000037         1570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_3720, whole genome shotgun sequence.
ACCESSION   DATGDI010000037 DATGDI010000000
VERSION     DATGDI010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3720"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1570
                     /locus_tag="VKO45_01355"
     CDS             <1..>1570
                     /locus_tag="VKO45_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HMA04552.1"
                     /translation="PAHERRKQALHPPHLLITTPETLQAVFMGKVLRKHLAHVRHVIV
                     DEIHELAGSKRGAQLSVALERLAVYAGDFQRIGLSATVGNPAEVAAFLCGDRPYRLVE
                     IPVTPHLDIRVRYAGEDFADQARYVGRCIGGKGSSLVFVNTRVTAEALGHQLYETGDV
                     EVHHGSLSKEVRVDAEERFKKGEIHTLICTSSMELGIDIGRVGHVIQFGSPREVSRLV
                     QRVGRAGHQLHTISRGTILTTGFDDTLESMVIARMAKENRVEPVVPHTGSADTLANQV
                     AALAVEYGEISRARILSILSRSGCYPDPGALLDRVCEQMASHRLIRIDGDFITKTGRT
                     RKYLSLNLSMIHDERKIRVFDMVTRRTVATLDESFVVGWAYTGAVFIAKGQLWRILDM
                     EGDRITVEPAKKAKGELPSWEGEQIPVPFEVAREVGRLRRDRNFSRYPAHHRAVEFAS
                     AFLGRMDRVKDRIPDERLITLESSDEGVVANVCGGHKANEALARVLSILLSARHGTTV
                     GIEVGAYRFLLRLPS"
BASE COUNT          256 a          562 c          498 g          254 t
ORIGIN      
        1 cacctgcgca cgagcggcgg aagcaggccc tccacccgcc ccacctcctc atcaccacgc
       61 cggagaccct ccaggccgtc ttcatgggca aggtgcttcg gaaacacctc gcccatgtcc
      121 ggcatgtcat cgtggacgag atccacgagc tcgccggttc gaaacggggc gctcagctct
      181 ccgtcgccct cgagcgcctc gccgtgtacg ccggggactt ccagcggatc ggcctctcgg
      241 ccaccgtggg gaacccggcc gaggtcgccg cctttctctg cggggaccgt ccgtaccgac
      301 tcgtggagat cccggtcact ccccacctcg acatccgcgt ccggtatgcc ggagaggact
      361 tcgccgacca ggcccggtac gtcggacggt gcatcggcgg gaagggctcg agcctcgtct
      421 tcgtgaacac ccgggtcacc gccgaggccc tgggccacca gctctacgag accggggacg
      481 tggaggtcca ccacggctcc ctctcgaagg aggtgcgggt cgacgccgag gagcggttca
      541 agaaggggga gatccacacc ctcatctgca cctcctccat ggagctcggc atcgacatcg
      601 gccgggtggg gcatgtcatc cagttcggct ctccccgcga ggtctcgcgc ctcgtccagc
      661 gggtcgggcg ggcaggccac cagctccaca ccatctcccg ggggaccatc ctcaccaccg
      721 gttttgacga caccctggag tccatggtca tcgccaggat ggcgaaggag aaccgggtgg
      781 aacccgtggt cccccacacc ggttcggcag acaccctcgc gaaccaggtg gcggccttgg
      841 ccgtcgagta cggggagatc tcgcgggcaa ggatcctctc catcctctcc cggtcgggat
      901 gctatcctga ccccggtgcc ctcctcgatc gggtctgcga gcagatggcc tcccaccggc
      961 tcatccgcat cgacggtgat ttcatcacga agaccggccg gacgaggaag tacctgtccc
     1021 tgaacctctc catgatccac gacgagcgga agatccgggt cttcgacatg gtcacccggc
     1081 ggaccgtcgc gaccctcgac gagtccttcg tggtcgggtg ggcctacacc ggcgcggtct
     1141 tcatcgcaaa gggccagctc tggcgcatcc tggacatgga aggggaccgg atcaccgtgg
     1201 aaccggcaaa gaaggcaaag ggggagctcc cctcctggga gggcgagcag atcccggttc
     1261 ccttcgaggt cgcccgggag gtggggaggc tccgccggga ccgaaacttc tcccggtacc
     1321 ccgcgcatca ccgggccgtc gaattcgcct cggctttcct cgggcggatg gacagggtaa
     1381 aggaccggat ccccgacgag cgtctcatca ccctggaatc gtcggacgag ggcgtcgtgg
     1441 cgaatgtctg cggcggccac aaggcgaacg aggcccttgc ccgggtcctc tccatccttc
     1501 tctccgcccg tcacgggacc accgtcggta tcgaggtggg ggcctaccgg ttcctcctcc
     1561 ggcttccctc
//
LOCUS       DATGDI010000038         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_323748, whole genome shotgun sequence.
ACCESSION   DATGDI010000038 DATGDI010000000
VERSION     DATGDI010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323748"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..416
                     /locus_tag="VKO45_01360"
     CDS             195..416
                     /locus_tag="VKO45_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04553.1"
                     /translation="MVYLPVANFFIYGIHLAIYLGAATFISLITTVTLGMLVLREGSN
                     VPFSWHLNMARVTIALALVHITVVYLTFF"
     gene            complement(460..939)
                     /locus_tag="VKO45_01365"
     CDS             complement(460..939)
                     /locus_tag="VKO45_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HMA04554.1"
                     /translation="MSTKLSGTGIAILGILLLFGVMAAGCTSSPSGPATTTPATTLAT
                     APPTTARTTVATTVPATTAPATTATTVQTPAPIQMAAVTIQNFAFSPASVTISAGSTV
                     TWTNQDAASHQIASDTMAFMGNPMSQGTSYSFTFMTRGTFPYHCAIHPSMKGTITVT"
     gene            1134..1703
                     /locus_tag="VKO45_01370"
     CDS             1134..1703
                     /locus_tag="VKO45_01370"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HMA04555.1"
                     /translation="MQVLDLALGVVWLVFWAYWFITAALTRSPFKRTQSCSRLLLFTI
                     ISVLALAILVEELPQGILVRDVLPGGAATMLVGLPITILGLGVAVWARVHLGRNWTSR
                     PGIKVGHTLIRTGPYRFVRNPIYTGLLIGYTGTAIVIGTLWALFLILFVLGASLLKIR
                     EEERLLLEEFGEDYEQYRREVRALIPYIL"
     gene            complement(1707..1883)
                     /locus_tag="VKO45_01375"
     CDS             complement(1707..1883)
                     /locus_tag="VKO45_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04556.1"
                     /translation="MTIEPKKGKDGQIEFTVNYMDPKTDNTFTITITNSLDEAVERLK
                     STLEAEVQAMREKK"
     gene            complement(1930..>2817)
                     /locus_tag="VKO45_01380"
     CDS             complement(1930..>2817)
                     /locus_tag="VKO45_01380"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HMA04557.1"
                     /translation="DEELATAPATLVMQYSTGRFGITDEKLRKAAAVEIRFGQGAYPG
                     KGSYLPAEKITPDIARIRGLAEGEAAYSPARHPDMRDAGEIRGKIAWLKALTGGIPVG
                     AKIGCGNIEDDVADLARAGADFIALDGFGGGTGATELAVRENVGIPLLAALPRAHRLL
                     RSLGLRERITLIGGGGLRTSADFAKCLALGADAVYVGTAAMIAVNCEQYRICHTGRCP
                     TGVTTQDPALEGQLDVPTGIDRLCNYIRVSTEEMAEFARITGKNDVHALDTADLVALE
                     EELARIVGCAWIGWAGTKE"
BASE COUNT          438 a          893 c          886 g          600 t
ORIGIN      
        1 cctttttttt atctcaaatg ctctcccgtc agggcagttc tccgtcaatc gggacaatga
       61 tggccgtcac cgctcgtcgg ggaatcaggg ggtctcacga cgaaaactcg tctttcccag
      121 gctgaacaat ggccgcggag cctgttcagg ataatatcga gagggttaaa tgggtacagg
      181 agcatgctac acctatggtc tacctccccg ttgccaactt tttcatctac gggatccacc
      241 tggccattta cctcggagcc gcgaccttca tctcgcttat caccacggtg actctgggaa
      301 tgctcgtcct gagagagggg tcaaacgtcc cgttttcctg gcacctgaac atggcgcggg
      361 tgaccatcgc cctcgccctc gtccatataa ccgtcgtgta cctgaccttt ttctaaaggc
      421 tcgggcaaaa agagagatgg tgtgggggtc ggggaacctt cacgtgaccg tgatggtccc
      481 cttcatggag gggtggatgg cgcaatgata ggggaatgtg ccccgggtca tgaaggtgaa
      541 ggagtagctc gttccctggc tcatggggtt ccccataaag gccatcgtgt cgctcgcaat
      601 ctggtgagat gccgcgtcct ggttcgtcca ggtgacggtt gatcccgcag atatcgtgac
      661 cgatgccggg ctgaaggcga agttctggat ggtgacggcg gccatttgaa tgggcgcggg
      721 ggtctgcacc gtcgttgccg tcgttgccgg ggcggtggtg gccgggaccg tggtggccac
      781 ggtggtcctt gcggtggtgg gcggagccgt cgccagcgtc gtcgccggag ttgtcgtggc
      841 cgggccggag ggcgagctcg tgcaccccgc tgccattacg ccaaagagga ggaggatccc
      901 gaggatcgcg ataccggttc ctgaaagttt cgtgctcata gtgatacaca cgggatgatc
      961 accccggcct tataaaactg actccccttg ccacggccat catcctcctg cccctcctgg
     1021 gcataaagta ggcgtcctgg ctacaaagcc acccctgcag gtccgatgcc acggcgggaa
     1081 tccccgcgaa gttatttgag cccgggaggg ggacatgaga aggggagaga gggatgcagg
     1141 ttctcgatct tgcccttggg gtcgtctggc ttgtcttctg ggcgtactgg ttcatcaccg
     1201 cggcgctgac ccggtccccc ttcaagcgca cgcagtcctg ctcgcgcctc ctgctcttca
     1261 cgatcatctc ggtcctcgcc ttggcgatcc tcgtagaaga gctgcctcag ggcatcttgg
     1321 ttcgggatgt tcttccagga ggcgcggcca ccatgctcgt cgggctcccg atcaccatcc
     1381 tcggcctcgg cgtcgcggtc tgggcgcggg ttcacctggg gagaaactgg acctccaggc
     1441 ccgggattaa ggtcggtcat accctcatcc ggacagggcc ctaccggttc gtgcgaaacc
     1501 cgatctacac gggccttctc atcggataca ccggtacagc catcgttatc ggaaccctgt
     1561 gggccctctt cttgatcctc tttgtcctcg gggcctccct gctgaagatc cgggaggaag
     1621 agaggctcct cctcgaggag ttcggagagg actatgaaca gtaccggagg gaggtgaggg
     1681 ccctcatccc gtatatcctg tgagatctac ttcttctcgc gcatcgcctg gacctcggcc
     1741 tcgagggtgg acttcagccg ctccaccgcc tcgtccaggc tgttcgtgat ggtgatggtg
     1801 aaggtgttgt cggtcttcgg gtccatgtag ttcacggtga actcgatctg gccgtccttt
     1861 cccttcttgg gttcgatggt cattttcgcc atggtgatcc ggagtatgta tgcgcctttc
     1921 cttttctcct tattcctttg tgcctgccca tccaatccag gcgcatccca cgatccgggc
     1981 gagctcctcc tccagggcca cgaggtcggc cgtgtccagg gcgtggacgt cgtttttccc
     2041 cgtgatccgg gcgaattccg ccatctcctc cgtggagacc cggatgtagt tgcagagccg
     2101 gtcgatgccg gtcgggacat cgagctgccc ttcgagggcc gggtcctggg tcgtcacccc
     2161 ggtcgggcag cggcccgtgt gacagatccg gtactgctcg cagttcacgg cgatcatggc
     2221 cgcggtcccg acgtacacgg cgtcggctcc cagggcgagg cacttggcga agtccgcgga
     2281 ggtccgcagt cccccgccgc cgatgagggt gatccgctcg cgcagtccca gggagcggag
     2341 gaggcggtgg gccctgggga gagccgcgag gagggggatg cccacgttct ccctcactgc
     2401 gagctccgtc gcccccgtcc cgccgccgaa gccatcgagg gcgatgaagt ccgcccctgc
     2461 ccgggcgagg tcggcgacgt catcctcgat attcccgcac ccgatcttcg cccccaccgg
     2521 gatgccaccc gtcagggcct tgagccaggc gatcttcccc cggatctccc cggcgtcccg
     2581 catgtccggg tgccgggccg gcgagtatgc ggcctccccc tccgcgaggc cccggatccg
     2641 agcgatgtcc ggagtgatct tctcggcggg gaggtagctc cccttgcccg ggtacgcccc
     2701 ctgcccgaac cggatctcca cggctgccgc cttcctgagt ttctcgtcgg tgatcccgaa
     2761 cctcccggtc gagtactgca taacgagggt ggcgggcgcc gtggccagtt cctcgtc
//
LOCUS       DATGDI010000039         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_352825, whole genome shotgun sequence.
ACCESSION   DATGDI010000039 DATGDI010000000
VERSION     DATGDI010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352825"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VKO45_01385"
     CDS             complement(<1..220)
                     /locus_tag="VKO45_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04558.1"
                     /translation="MATVSETGNSGMAIAAAIVAGVVVYFLTLEASWAAVAAIVIGYL
                     VMGIRIVRPTHRVLIEFLGKYQKYGNPGL"
     gene            complement(430..1284)
                     /locus_tag="VKO45_01390"
     CDS             complement(430..1284)
                     /locus_tag="VKO45_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMA04559.1"
                     /translation="MKGIDLISHAKNTWCPGCGNFAIQHILKDLLAAMETEGFPVEKN
                     VLVAGIGQHGKLFDFMNLNGFYSLHGRTIPVAMGIRLANPDLRVICVTGDGDSYAEGL
                     DHLIFAAKRNLDITVLVHDNRVYGLTTGQFTPTSPYGYRGHSTPAGDREQPLNPLELM
                     LASGATFIARGYTGRREELSRLIREATLHRGFSFVDILQVCATYCDLTQYYNERVYAW
                     TEGDVEDRDAACRRAREWDYSADAKIGLGVMYRKAASTFEDGYPVPSALTHEERAGWV
                     ARIMGERS"
     gene            complement(1281..>2526)
                     /locus_tag="VKO45_01395"
     CDS             complement(1281..>2526)
                     /locus_tag="VKO45_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HMA04560.1"
                     /translation="WPVAIGVLGKSFRKATEQNLRVARRGYEASEERLRIPSLGLPPL
                     PAFTGNEAMGIGLVEAGLGVYISYPMSPTSNLMHFLAAHADETRIRVLQPESEIAVIL
                     ASLGCAYAGTRAAVGTSGGGFSLMVEALGLAGMAELPVVIVLGQRAGPSTGLATYTAQ
                     ADLEFAIHAGQGGFPRLVIAPGDAEEARTWIRAAVDLAWKYQLPALFLPDRIVCEGMY
                     SLDPGVAPEYQAFPAPADPGTHPYLRYAITDSGISPLRFPPVKGEVIRVNSHAHDTDG
                     ITTEDPGVTRDMADKRWRKMAGLAREIEGMHPVNVSGDPEGATAILCWGSNKGVCGEC
                     GERIGLRVVQPVVLWPFPEESFAHAMKGVERVFGVETNETGQLSSLVGRFGYRAERRI
                     LKYDGRPFMVDELEQELRRVLA"
BASE COUNT          440 a          820 c          821 g          445 t
ORIGIN      
        1 acagccccgg gttgccgtac ttctggtact tccccaggaa ctcgatgagc acccggtggg
       61 tgggcctcac gatccgtatc cccatgacga gatagccgat cacgatggcg gcgacggccg
      121 cccaggacgc ttcgagggtg aggaagtaga caacgacccc cgcgacgatg gctgcggcga
      181 tggccatgcc cgagtttccc gtttcgctca cggttgccat ggtaaacctg ttataacttt
      241 tcacccataa tcctataccc attttatttg tacgggtctc aagcagggcg aaggaaggaa
      301 aatgggtcaa catttgaaaa aataacggat cccgtgccct gccccgcaat cctgcccggg
      361 aaaagggata tatgccgaac cgcaagataa ggggtgggaa tcttctcctg atgtctgcaa
      421 ccggagaggt caggacctct cgcccatgat ccgggcgacc catccggccc tctcctcgtg
      481 ggtcagggca gagggcacgg ggtatccgtc ctcgaaggtg gaggcggcct tccggtacat
      541 caccccgagc ccgatcttcg catcggcaga ataatcccat tcccgcgccc gccgacaggc
      601 ggcatcccgg tcctccacgt cgccttctgt ccaggcgtac acgcgctcgt tgtagtattg
      661 ggtgagatcg cagtacgtgg cgcagacctg caggatatcc acgaaggaaa acccccggtg
      721 gagggttgcc tcgcggatca ggcgtgagag ctcctcccgc ctgccggtgt aaccccgggc
      781 gatgaaggtc gcccccgagg cgagcatgag ctcgaggggg ttcaggggct gttccctgtc
      841 cccggcgggg gtggaatggc cccggtaccc gtacggggag gtgggggtga actgccccgt
      901 ggtcagcccg tagacccggt tgtcgtgcac gaggacggtg atgtccaggt tcctcttcgc
      961 cgcgaagatg aggtgatcca ggccttcggc gtacgagtcg ccgtcaccgg tcacgcagat
     1021 caccctgaga tcggggttcg cgagccggat ccccatggcc accggtatag tgcggccatg
     1081 gagggagtag aagccgttca ggttcatgaa gtcgaagagc ttcccgtgct ggccgatgcc
     1141 cgcgacaagc acgttcttct cgaccgggaa cccctccgtt tccatggcgg cgaggagatc
     1201 cttgaggata tgctggatgg cgaagttccc gcacccgggg caccaggtgt tcttcgcatg
     1261 gctgatgaga tcgatcccct tcatgcaagc accctccgga gctcctgctc cagttcgtca
     1321 accatgaagg gcctgccatc gtacttcagg atccttctct cggcccggta cccgaaccgg
     1381 ccgacgagag aggagagctg gccggtctcg ttcgtttcca cgccaaacac cctctccacg
     1441 cccttcatgg catgggcaaa gctctcctcg gggaagggcc agagaacgac cggctggacg
     1501 acccggagcc ctatcctctc cccgcactcg ccgcagactc ccttgttcga gccccagcag
     1561 aggatcgcgg tcgccccctc cggatctccg gagacattca ccgggtgcat cccctcgatc
     1621 tcccgggcga ggccggccat tttcctccat cgtttgtcgg ccatgtcccg ggttaccccg
     1681 gggtcctctg tggtgatccc gtcggtgtca tgggcatgac tgttcacccg gatcacctct
     1741 ccctttaccg gcgggaaccg gaggggcgat atccccgagt cggtgatggc gtaccggagg
     1801 tagggatggg tccccgggtc ggcgggagcc gggaacgcct ggtattccgg ggccaccccg
     1861 ggatcgagac tgtacatgcc ctcgcagacg attcggtcgg ggaggaacag ggcgggcagc
     1921 tggtacttcc aggcgaggtc cacggcggcc cggatccagg tccgtgcctc ctccgcgtcg
     1981 cccggggcga tgacgagcct cgggaagccg ccctggccgg catggatggc gaactcgagg
     2041 tccgcctgtg cggtgtaggt cgcaaggccg gtgctcggcc ccgcccgctg cccgaggacg
     2101 atgacgaccg ggagctcggc catcccggcg agccccagag cctcgaccat cagggagaaa
     2161 ccgcctccgg aggtccccac cgcggcccgg gtcccggcat aggcgcaccc gagagacgcg
     2221 aggatgaccg cgatctcgct ctcgggctgg agcacccgta tccgggtctc gtcggcatgg
     2281 gcggccagga aatgcatgag gttcgaggtg ggggacatgg ggtacgagat gtacaccccg
     2341 aggccggcct cgacgaggcc gatgcccatg gcctcgtttc ccgtaaacgc cgggaggggg
     2401 ggcagcccga gagaggggat gcggagcctt tcctccgagg cctcgtagcc cctcctggcg
     2461 acccggaggt tctgttcggt ggccttccgg aaggatttcc cgagcacccc gatagcgacg
     2521 ggccac
//
LOCUS       DATGDI010000040         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_265738, whole genome shotgun sequence.
ACCESSION   DATGDI010000040 DATGDI010000000
VERSION     DATGDI010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265738"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VKO45_01400"
     CDS             <1..845
                     /locus_tag="VKO45_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HMA04561.1"
                     /translation="DVVVAEATCDGKKKMYEVLSSHIPTYVLDLPQKPESPEALAYFA
                     SELRRFGRFMEDLAGRTITDDALGREIRSANETRRLLHRLFGLRMADPPPIRGSEVLR
                     VMQKQFFLSPVQFREGITRLCDELEHTVPPPRKGPRILISGCPMASGNTKMVEILEEK
                     GAAVVAEESCTGTRSFWDPVDEKGDPWKALAERYLGITCACMTPNKRRVDRIVDLAKQ
                     FRADGVVYYTLQFCHGYNIERLLVQQALKKERIPLLAIESDYGDSDREQIGVRVDAFL
                     EMIA"
     gene            842..1630
                     /locus_tag="VKO45_01405"
     CDS             842..1630
                     /locus_tag="VKO45_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HMA04562.1"
                     /translation="MITAGIDAGAATTKVVLLRGSEIAGYRIENTRYDFLTVAEAAFR
                     DLLSDSQIGRDEVRGVWATGYGRGSITFAERTVSEITAHARGVNLLYPGVRGIIDVGG
                     QDSKIIVLEDGRVTDFLMNDRCAAGTGKFLEYTARSLEVPVELLGTLALASTRPARIT
                     SMCTVFAESEVISLRARGATREDIAAGLIGSIAERIVSMAKRMGLSGRIAFVGGVAKN
                     AAMRKAIEKGLGVTLFVPPEPQITGALGAALAARGAGERGDPTG"
     gene            1677..2516
                     /gene="ygiD"
                     /locus_tag="VKO45_01410"
     CDS             1677..2516
                     /gene="ygiD"
                     /locus_tag="VKO45_01410"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HMA04563.1"
                     /translation="MIGLSAMVTRGNPSGRERMPVLFIGHGSPMNMVLSNRFTKSLAE
                     LGPSLPRPDAIMVISAHWLTHGTFAGCMERPRMIYDFYGFPRELYQVKYECRGAPGHA
                     ARAAELGRADGPIRCDKDWGLDHASYSILMHMFPAADIPVFEMSLDYSFNDWHPKPVQ
                     FHYDLGKVLRPLRNEGVLVIGSGNMVHNLNLIDFSDMDAAYDWAVEADSRMKAHLVKG
                     EHRELIDYLTMGKGASLAVPTLDHYLPMIYALALQDEGESLQFVHEGFQNGSVSMRSF
                     RIG"
BASE COUNT          572 a          872 c          932 g          506 t
ORIGIN      
        1 tggatgtggt ggtggcagag gcgacctgcg acgggaaaaa gaagatgtac gaggtcctct
       61 cctcccatat ccccacctac gtcctcgatc tcccgcagaa acccgaaagt cccgaggccc
      121 tcgcctactt cgcctcggag ctccggaggt ttggacggtt catggaggac ctggccggga
      181 ggaccataac cgacgatgca ctggggagag agatccgatc ggcgaacgag acccggaggc
      241 tcctccaccg gctgttcggc ctgaggatgg cagaccctcc ccccatccgg ggctcagagg
      301 tcctccgggt gatgcagaag cagttcttcc tctcccccgt gcagttccgg gaggggataa
      361 cccggctctg cgatgagctg gagcacactg tcccacctcc ccggaaagga ccgaggatcc
      421 tgatctccgg ctgcccgatg gcctcgggaa acacgaagat ggtcgagatc ctcgaggaaa
      481 agggggctgc cgttgtcgca gaggagagct gtaccggcac gagatccttc tgggaccccg
      541 tggatgagaa gggggatccc tggaaggccc tcgccgaacg gtacctgggg atcacctgcg
      601 cctgcatgac ccccaacaaa cgacgcgtcg atcgcatcgt cgacctggca aagcagttcc
      661 gggccgacgg ggtcgtctat tacacgctcc agttctgcca cggctataac atcgagcgcc
      721 tcctcgtaca gcaggccctg aaaaaggaga ggataccgct cctcgccatc gagtccgact
      781 acggcgattc ggaccgggag cagatcgggg tccgggtgga tgccttcctg gagatgatcg
      841 cgtgatcacg gccggcatag acgcaggtgc cgccacgacc aaggtggtac tgctccgggg
      901 aagcgagatc gccggatacc ggatcgaaaa caccaggtac gacttcctta ccgtcgccga
      961 ggcagcgttc cgcgatctcc tctccgattc gcagatcggg agagacgagg ttcgcggggt
     1021 gtgggctacc gggtacggcc ggggctccat caccttcgcc gaaagaaccg tgagcgagat
     1081 caccgcccac gcccgggggg taaacctcct ctatccgggg gtgcggggga tcatcgatgt
     1141 cggagggcag gactcgaaga tcatcgtcct cgaggacggg agggtcacgg atttcctcat
     1201 gaacgaccgg tgtgccgccg ggaccgggaa gttcctcgag tataccgcga gatccctcga
     1261 ggttcccgtc gagctcctgg gcaccctcgc cctcgcgtcc acccgccccg ccaggatcac
     1321 gagcatgtgc acggtgttcg cggaatcgga ggtgatctct ctcagggcac gcggggccac
     1381 ccgtgaggac atcgccgccg ggctcatcgg gagcatcgcg gagaggatcg tctcgatggc
     1441 aaagaggatg ggtctctcgg ggaggattgc cttcgtggga ggggtggcga agaacgccgc
     1501 gatgaggaag gccatcgaga agggcctcgg ggtcaccctc ttcgttccgc cggaaccgca
     1561 gatcacgggg gccctcgggg cggctctcgc cgcccgggga gcaggggaaa ggggagatcc
     1621 taccggatag ccccgaccac agaccggcag agcataggct aaaggtgccc ggcgtgatga
     1681 taggtctgag tgccatggtg accagaggga acccttccgg ccgggaacgg atgcccgtgc
     1741 tcttcatcgg ccatggatcg cccatgaaca tggtcctctc gaaccggttc acgaagagcc
     1801 ttgcagagct gggcccttca ttgccgaggc cggatgccat catggtcatc tcggcccact
     1861 ggctcaccca cggaaccttt gcaggctgca tggagaggcc ccggatgatc tacgatttct
     1921 acgggttccc ccgggagctc taccaggtga agtacgagtg caggggagcc cccggccatg
     1981 cagcccgcgc ggccgagctg ggaagggccg acgggccgat ccggtgcgat aaggattggg
     2041 gcctggatca cgcctcctac tcgatcctca tgcacatgtt ccccgcggcc gacatccccg
     2101 tcttcgagat gagccttgac tactccttca acgactggca cccgaagccc gttcagttcc
     2161 actacgatct cgggaaggtc ctccggccac tcaggaacga gggtgtcctt gtcattggca
     2221 gcgggaacat ggtccacaac ctgaatctca tcgacttctc cgacatggat gccgcgtacg
     2281 actgggccgt agaggccgac tcgaggatga aggctcacct ggtgaaggga gagcaccggg
     2341 agctcattga ttacctcacc atgggaaagg gggcatccct cgctgttcca accctcgatc
     2401 attacctgcc gatgatctat gcccttgccc tccaggatga gggggagagc ctgcagttcg
     2461 ttcatgaagg cttccagaac gggtccgtct cgatgaggtc cttccggatc ggctgagaac
     2521 ccccgttcgg tatcgggatc catctccacc ggggaaatga gggagcagag ccgggtatcc
     2581 gtcgcttctt ctccaggtaa ataaccggcc aatccctctc atccgggaca ggacggtggg
     2641 gattggagag agggacccga atccggtctc cttgtgccat cctccttcag gaatacctct
     2701 tttttcccga tggccgtcgc agcgcagcat gcgggaggtg ggagggggtg ccagtaaaga
     2761 tataaaccaa ttttccagat atataaagag agcaattttt tgccggccgg tgcgatgaga
     2821 gggaacccga tgtcgccggg gtgagggaat ggaggcgccg cgtgagagga agatcggcgg
     2881 ga
//
LOCUS       DATGDI010000041        32735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_323892, whole genome shotgun sequence.
ACCESSION   DATGDI010000041 DATGDI010000000
VERSION     DATGDI010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 32735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32735
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323892"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1872)
                     /gene="thsA"
                     /locus_tag="VKO45_01415"
     CDS             complement(214..1872)
                     /gene="thsA"
                     /locus_tag="VKO45_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HMA04564.1"
                     /translation="MMSQLAGQPILILKEGSSRTRGRDAQGSNIAAAKAVAGAVRTTL
                     GPKGMDKMLVDTIGDVVITNDGVTILKEMDIEHPAAKMMVEVAKTQDDEVGDGTTTAV
                     VVAGELLKRAEELLDQDVHPTVIAQGYRLAAEKAREILNDIAITVKPTDKEMLFKIAE
                     TAMTGKGAEASKDKLCELVVKAAMMVVDEDGTVDKDNVKIEKKVGGSSDDSMIVEGVL
                     IDKERVHPGMPKKVENAKILLLNASIEFKKTEVDAEINITSPDQLQAFLDEEERMIKS
                     IVDKVVASGANVVICQKGIDDIAQHYLAKANILALRRVKKSDMEKLARATGASVISSI
                     DAISKSELGKAGLVEERKVGGEEMIFVEKCVNPKAVSIIVRGGTEHVVDELERALVDA
                     ISVVSVVMEDKKLVAGGGAAEIELSLRLRDYAASVGGRAQLAIEAFANALEIIPRTLA
                     ENAGLDPIDMLVALRAEHDKKRKNYGLDVFKGAPIDMYKAGVVEPLRVKTQAVASAAE
                     AAVMILRIDDVIAASRVAGAPPGGPGGMPPGGMPPGMGGDMGDF"
     gene            complement(1978..2898)
                     /locus_tag="VKO45_01420"
     CDS             complement(1978..2898)
                     /locus_tag="VKO45_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HMA04565.1"
                     /translation="MTQEVLLQRVRSLLVEAGFQVSDRCSQRPRSFDLIAGDREVLMV
                     VKAISHIDGVSEDIARDLDLVSYYLGGAPLIVGERTRDADLQRGAVYVRYGIYALSLP
                     TLSDFLMEGIPPLIYVSPGGLYVNIDEKLLRALREERELSLGDLAHLLGVSRRTISKY
                     ENGMGTTMEIAVRIEEVFDTAITKPVDLLSHESRFARADEPARGQVLPDLERLGMEIH
                     LLRRAPFEALARYDDETILTGYGTAQHVEKRAPLIGSISRIARARALCVLFGYRRQKT
                     IGDTLVIGEERLHALEDGSELMELIREPPA"
     gene            2947..4170
                     /locus_tag="VKO45_01425"
     CDS             2947..4170
                     /locus_tag="VKO45_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)(2)-agmatinylcytidine synthase"
                     /protein_id="HMA04566.1"
                     /translation="MWIGIDDTDSPRGMCTTYLGAVLIRRLLSQRFALHEARLIRLNP
                     NVPWKTRGNAAISLEVGGDPEVAFGMACRCVDEMADLPAEGTEPGVAVSETKPPAGFY
                     EKALRSFCERGEAIEVLEEAGAMYRGWKGGRGLIGATAAIASTLPDSTWELLVYRKPD
                     LQGTPRNIDRGSFFLADASTAPATWDTVDRENGIVVCSPHTPDPVLFGIRGRSPFSVA
                     LARTFIRTEPAGMEQVYLTNQGTDGHLEEGVVGGLSEGHSYRVQGIVSGRAATGRGGH
                     VSVPIGDGGLSLRCMAYEPTKGFRDMVRALVPGDEVEACGSFKGGSLNLEKIRLIEPA
                     QDLVMRSPLCPACGVRMTSAGRGKGYKCRRCGERAAGGEVQERARGIAAGWYEVPPSA
                     RRHLAMPLARGIRHR"
     gene            4208..5137
                     /locus_tag="VKO45_01430"
     CDS             4208..5137
                     /locus_tag="VKO45_01430"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HMA04567.1"
                     /translation="MEGDAVKQVRIRPGMTAGELASELEASGAFNGGSLAKACRITEA
                     MFRDGKATTFLGLAGAMVPAGMGGIVADLIDRGYVDVLVSTGANLVHDMVEAIGCRHY
                     HGSAECSDNELRRKGINRIYDVYVSDDAFTQFEDFIQGVFGGLSDGTVISTSELLGMV
                     GKKLPSGILATAAKKGVPVYCPAVQDSVFGLQFSLFSQTHKVTVDAFRDMKGFLDRCF
                     TSESAGALLIGGGVPKNFIFQGMLLAPHGFTYAVQLTGDRPDLGGLSGATLDEARSWG
                     KLTPEAQGVTVYGDATITLPFLAAAVLERLSRG"
     gene            5130..5765
                     /gene="pyrF"
                     /locus_tag="VKO45_01435"
     CDS             5130..5765
                     /gene="pyrF"
                     /locus_tag="VKO45_01435"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HMA04568.1"
                     /translation="MAELILSLDVTDRARAVDIARACAPHLDAIKIGYPLVLSAGISI
                     AKDLLPLALPLIADFKVADIPDTNRTICTLAFDAGFSGVIAHAFPGEDALSACVEVAH
                     ARGGACYAVAEMSHPGAAAFFHGGVAERLAALAVRCGADGIIAPATRPGRVARLREIA
                     GSLKILSPGVGAQGGELEAVKDLVDGIIVGRSIFAAPDPKAAAEDFSRRCR"
     gene            5933..6295
                     /gene="nrdD"
                     /locus_tag="VKO45_01440"
     CDS             5933..6295
                     /gene="nrdD"
                     /locus_tag="VKO45_01440"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HMA04569.1"
                     /translation="MQWNPEQRKLIEKYHSIQDIPPGERRYKCFTCHTIVDTSPCPRC
                     GEARLQILCPLDHAQCSHEIIARIEYCPMCGNPVCPDCGSHDVVQISRVTGYLQDVSG
                     WNAGKQQELKDRVRYTLS"
     gene            complement(6358..7878)
                     /locus_tag="VKO45_01445"
     CDS             complement(6358..7878)
                     /locus_tag="VKO45_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HMA04570.1"
                     /translation="MGALLLIAMALWCGAVSAAQAAANLQLTKTLHNEGSITAGDQII
                     WNITLTNSGPDAAPEVTVTDDMSLLRDVTLDLAEASDGTTYDQNAHTWTVPPLTGDGA
                     SATLNLTMTLAEDAAPGEIKNCVSITAPSEEISETPVCASATITAEAADLELTKTLFN
                     TGPIFAGDQVIWNITLTNQGTAGVTDIVVTEDPSALGDFTLVRAEASAGSAFDDATNT
                     WTIDSLAGNGATATLNLTMTLAEDAIAGELKNCAKITSPAQAGSEPVCTSGETSPTVK
                     LDVTIKPETLNLKSRGVFTAFIRLAGDTSQEGITLRDISSITCGGAPSTKVKMNQKAG
                     GTLMAKFRRQSLGKDVVAGETVEIVCNGTVTKGGETVKVIGSDTIRVIGEKKTGLDAF
                     FSDILDTVAPLDEVGEGTDETSTSPTATPTVSPDQNQNRGQLKKQERNQDGSCTENCA
                     TGNSKNIKGNNGKKSASDDEDDQADTQGQGNQGNGNGNGKGNKDKEKGQGNGNNKK"
     gene            8205..9077
                     /locus_tag="VKO45_01450"
     CDS             8205..9077
                     /locus_tag="VKO45_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HMA04571.1"
                     /translation="MRYFFRGGTLFVRGDFQAASTGVNGGLRRVTTLLNHTVGRDFPH
                     EEPLRSLEDLVAGMGFSRDFFGLLTAVGIRHTCILQYDFITLFVTAGITGPVAETPAG
                     TINIIIHSAEGFSDGALLEALLVSTMGKADALRALGHPFSGTPTDAVVAACEGPARHE
                     YAGTATEGGRRIYEAAFHGVEEALARHERRVERDAPSLFIYSRYGGDHWVEWRPEGCP
                     YYPCHFEGQRCDYCYCPLYPCLDPTLGQWVESKNGGRVWNCSGCTLLHIPKVTEYLKK
                     NPEATLRELKRAAG"
     gene            complement(9098..9292)
                     /locus_tag="VKO45_01455"
     CDS             complement(9098..9292)
                     /locus_tag="VKO45_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HMA04572.1"
                     /translation="MAITADSTIMEVLQAKPDAANVLFKFGMGCLGCAVARGETIREA
                     AQAHGIPLKELLSALGIPEN"
     gene            9475..10656
                     /locus_tag="VKO45_01460"
     CDS             9475..10656
                     /locus_tag="VKO45_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HMA04573.1"
                     /translation="MEAKGIAVDRPRIESKIRRLVEEFGVQLAEAERTVWNEIAREHS
                     LPSPGGRAQEREIRGLSAGEWVTVQGKVVALTSPPTPAIAQAGILADPSGAIRFTVWA
                     RAKAQPLSAGKWYRMESAVVDEFRGVEKLNIHSGTTITEVENEAPLIPKVVPIRELQP
                     GVGSVRVKVVQEWEASHERMLQTGLLGDETGTIKFTIWREEGKEKLTPGKVYSIYYAL
                     VDEFNGRLQLNLSPAIYLPEEGDIQVSEGETTRGVIVHLAPGSGLIKRCPVEGCNRVL
                     SRQNYCPVHEIQKTFRYDLRIKGVLDDGIRTRNILVQKEIVEALSGMKLEEAVALAES
                     SPLGMDEVFYRIRDAVLGRYFQARGTEMDGRILVKECRKLAYDPSDLAALLNRAGGEA
                     K"
     gene            10656..11246
                     /locus_tag="VKO45_01465"
     CDS             10656..11246
                     /locus_tag="VKO45_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HMA04574.1"
                     /translation="MNGRKAGGPRPGQTFDREPARRVFAGELREVRLQFREGEDEKSP
                     SFVLLPTGSRCNRIFLVGTLTEKDRRGDQNVFYHARISDPTGTFFLMAGSYQPEAMQQ
                     IARIEPPAFVAVIGKPNVFESQTGSFLVSVRAESVTAVDRETRDLWVLDAARATLQRL
                     DELGNTEDSKRAQEQYHPDPAVYRKMVYDALAQLKI"
     gene            11303..11890
                     /locus_tag="VKO45_01470"
     CDS             11303..11890
                     /locus_tag="VKO45_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HMA04575.1"
                     /translation="MEKNLLVPRVRAGAARAVRALPLIPLFILSGCLVPGARTGTPVL
                     EVSSAAFAPGGEIPVMYTCDGANVSPPLSWSAPPQGTESIAILVTDPDAPGGTFVHWV
                     AWNIPPGTREIPSGSAGKPVLPTGSVQGTSDFGRQGYGGPCPPGGKPHHYHFVVYALD
                     SALTLPGKRDGRTLESSVRGHILAQGETIGIYRRI"
     gene            complement(11958..12992)
                     /locus_tag="VKO45_01475"
     CDS             complement(11958..12992)
                     /locus_tag="VKO45_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3821 domain-containing protein"
                     /protein_id="HMA04576.1"
                     /translation="MTGSPSSREQISRAVVTGGVLVVLLWAILAVPSAARTSIQTIQN
                     GDMIFIYEQNLDITALRTGANPVASLRKYQDDDPTKALLREVAVSDDTSFSLIPEEFG
                     DLRGVYIAFNPAVGAMGRVVVQLPSVSIDAVLASPNHVDSIAGLTIPEDTPIAFKITS
                     VDVGSTYHAGSLFPATVDLVLTAPGGAQLTTILGRDFSAMHVSRQVFYTDDPGRPGAI
                     SLGSLGAGSYSVQAKWRDPASFENQAPDSNIITFTIGKKIAVQTTPTPVTTPVTTQTT
                     ATTVRQTIPPTTTRPVTTVGTPTPEATTLPPATGTTSPPPTPAPTGAWLAFLAPAMAA
                     LTLILKRRKG"
     gene            complement(13108..14178)
                     /locus_tag="VKO45_01480"
     CDS             complement(13108..14178)
                     /locus_tag="VKO45_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMA04577.1"
                     /translation="MFVDFLNRYRAKSTRSMYGSAWIKFLSLVYGARLKGASPALLDP
                     WVAKYVNDLRNGRRDLAADLRALEAACRSRRTCLGYRSAIRTLLAEAGIALPPGDARR
                     LPRARGSHRPPAAVDSLTPGKLLRLMEHLDTRGRAIVYTLVSSGAHLGEVLSIRLGDL
                     DLDARPARFLIRTGKTEAPTVAFISREAVGAIRVYLIVRDRFLASARARGGTAGEDLL
                     FPIGPRAFEQVWDLALSKAGLERVDRVTGRRTLTLRSARRFFADRMGTVLPATVVREL
                     MGSIRTPRGETAGQVEKDLERAYLAGEPVMTLHRTHRKESLSRKVAELERTVRFLRER
                     VEERDRKSAPLDSGTVEIGEDG"
     gene            14310..16595
                     /locus_tag="VKO45_01485"
     CDS             14310..16595
                     /locus_tag="VKO45_01485"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMA04578.1"
                     /translation="MKSRREREASRLREKGVRPQTAPPAEMRDPKAVDHLIRALSDKD
                     ETVRSQAARALGEMRDPKGVDHLIRALKDRHKLVRAEAAEALGRIGDPLAVDHLVRAL
                     KDENARVRGASARALGEIGDLQAVPAIIGTLKDRDEDVRGEAARALGKMGDERALQPL
                     IEALRDIDTYVRCYATIALGTIGTPALDSLIQALKSEYDDVRERAANTLVGMKDRAVE
                     SLIGALRDEDWAIRKEARRILAAIKAPAVDPLIKALRDEDWDVQRQVAQVLEEIGDDR
                     AVEGLVDVLKKAPWAAQKEIIRVLTSFGWSPLSDEERAYFLISKGSWTELEQIGDAAV
                     PALANALRDEDRYVRRQAARVLGGLGDRAAVEPLIEALEGEDAYLRSMAAESLGRIRD
                     KTAVPALLKALSADDSWVKGYAVEALGMIADGRAVESVIGVMKSGGDGIRRAGARALA
                     GMGKPAIKPLIQVLSGDDREMKESAAWVLAMAGELSVPPLIDSLKREGDDHRADAEEV
                     LFEIGEPAVDPLIAALGDSHEPARRSAARVLGRFGDRRAVEPLIRAIGDSSAGVRKEA
                     TIALGRIGEPYAVTPLVGLLADDELEVQREAVVALGKVRDIRALAPLIGSLRNPELRE
                     NVTEALSAMSDERAIVPLIEALKDESVFVRMGAADVLVHIGEPAVDPLIEALSHDDAG
                     VRYVATWTLGKIGDRRAVEPLIAAMKDRDKGVKKGASVALGDIGDGRAVEPLIQALKD
                     EDESIQRAARESLERIISKKD"
     gene            complement(16958..17656)
                     /locus_tag="VKO45_01490"
     CDS             complement(16958..17656)
                     /locus_tag="VKO45_01490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HMA04579.1"
                     /translation="MPPDGLDLEIREADVLRPTIRVLPSGTVQVTAPPGTHIPSLIEP
                     NLAWIERKRREIREISRDREGQEGRILLGGRFFRLENGPSCGIRAEEGGITYTTPGDL
                     KAFLKDRLRGSLLPRLEAHAIRMRVGFTGLSVREQRSRWASCSPAGRISVNLRVLALP
                     DDIRDYLVVHELAHLLEPNHSRRYWRRVAEFFPDYRHAEEELKRYWILLERDSIWEVL
                     GKVRERRGTENLSS"
     gene            complement(17664..18308)
                     /gene="wrbA"
                     /locus_tag="VKO45_01495"
     CDS             complement(17664..18308)
                     /gene="wrbA"
                     /locus_tag="VKO45_01495"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF002999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HMA04580.1"
                     /translation="MKILVVYYSMLGHVHRLAEAIAAGASGVDGVSVEMRRVPETLPP
                     EVLEKTGAFKAQKAFSHVPVCERGELGNYDAIVFGTPTRYGNMCGQMRQFLDATGSLW
                     VQGALAGKVGSVFTSSATQHGGQESTILSFHTTLLHHGMIVVGLPYLFAGQSRLDEIT
                     GCSPYGASTIAGSGGERWPTENELAGARFQGRYVALVASRITPHRDAIIASLTR"
     gene            complement(18383..19522)
                     /locus_tag="VKO45_01500"
     CDS             complement(18383..19522)
                     /locus_tag="VKO45_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMA04581.1"
                     /translation="MEEIALALFLCLILALLSKRYSLPPIPFYILAGIAIGKSGLSLV
                     PSDAYSQYLSSLGLIFLLFYVGLEMRPKSLRERGRSLLLSGGIDLSVNLAVGLAAAFL
                     LGFPPLESFILASAFYISSSAMAIASLVENRRLILPEAETIVWMMVFEDVLLILLISV
                     FSAQSENPVMFILLTVAVLAATVLVVRALRRPIHALLARTDDLPSLFTFTAVISSTYL
                     AKLLKIPDALVAIILGSSLAATSASALDRLSQPFREVFLVLFFVFFGITIDFSGGFSP
                     LVASVLCLLALGSKMASGILVGWRLHGSVPAGIEIGANTLGRGEFSIALAAVFGSAAI
                     SATVAILVVATSLVGSFAARYSEGMKVWPGRAEGNPHSAPGGQPE"
     gene            complement(19522..20007)
                     /locus_tag="VKO45_01505"
     CDS             complement(19522..20007)
                     /locus_tag="VKO45_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HMA04582.1"
                     /translation="MGVRCIELPGIGTKYELETEKGDRVAIIFMGSRKFQLYVLEKGK
                     GSPSAAELTAAEARRLGSLLSGAIIEAEKEAVEIAFSALSDLRIRVHTYVIRKNLAGR
                     SIAELQVRTKTGATVVAVSRAGKNIINPLPSFTFENGDIVVAIGDTDQLEEFEHQILG
                     N"
     gene            20200..22023
                     /locus_tag="VKO45_01510"
     CDS             20200..22023
                     /locus_tag="VKO45_01510"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HMA04583.1"
                     /translation="MPGKYMDLRRKLLIVVGVTLICTLIAFYAITHGAVLGAFVTLEE
                     RNTQQNMNRFMNLLSDDLSSLDSVVRAWAPRDDTYTFLRDGNTQYIEKNFRAETFTSN
                     RLNFMVFLNTSGDISYAKAFDLSSGQEESLPPSLAGMVAGNEILRSFSQNDASLSGFL
                     MLAEGPVMVASAPITTKNYEGVSRGTLVVGRFLDDNEIRRIAQLTNQQVMMYRRSDPG
                     SPADVREAQLELDGGRTIWIQPDSPGTIAGYAPVEDLYHTPILIIKTQIPRQIYAQGQ
                     ATLDYFTLSYIILILIFAITFLYLLDRSILSPISLLSTDVKRIGMTRDLSARLRVGKE
                     ENEISNLQENINKTLAALEKAQGRLQKSEEKNRALLAAVPDLMCQVRREGIHEEWNSG
                     TAAELGVMALHELLGNELEEIEETFELIPQEGQGLPDAGAGNGEVSPVFIFEYKTKGK
                     GGMRYHEIRLVVSGKDEVLAIGRDITERKQAEEELERYRARLEELVGARTQALQRTNE
                     QLRQEIFQRKLIEGQKREAYEQIEKNIEQFAILGDHIRNPLAVIVGLADLTEGAEAAK
                     IMEQARVIDGIINQLDRGWIESEKVREFLRRYYSNDKDSAK"
     gene            complement(22314..23105)
                     /locus_tag="VKO45_01515"
     CDS             complement(22314..23105)
                     /locus_tag="VKO45_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA04584.1"
                     /translation="MSRLGYLTPPHLTGRTLRVWQRNRKVFVKTFRVNFLPPLLEPLL
                     YLLSLGYGLGIFVSQINGVSYPRFIAPALLAISIMNAGFFETTYASYVRMYFQKTFDA
                     IIATPLSIEEVITGEILWGATRSVINATIMLPVLVLFGVVDLPISLSILPFAFLAGFL
                     FASIGMCFTALTPNIDTLNLPTFLFVTPMFLFSGTFFPLTILPAPIQVFATAFLPLTH
                     VVKITRMVTLSQVTPEILLSLAWIVGVAAVAFILAVNLMKKRLIV"
     gene            complement(23102..24019)
                     /locus_tag="VKO45_01520"
     CDS             complement(23102..24019)
                     /locus_tag="VKO45_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA04585.1"
                     /translation="MTGVADVILAENLEKRFGDFVAVDRVTFTVREGEVFGFLGPNGA
                     GKTTTMRMIQCVSPKDSGRLEVFGMDVSAHPAEIKGLIGVVPQETNLDPDFSVYENLM
                     VYSRYFDIPPSTAREAIDGLLEFVQLSERRDTSIEKLSGGMKRRLILARALINNPRLL
                     ILDEPTVGLDPQARHLIWDRLRALRSRGNTIVLTTHYMEEAAQLCDRIVIMDLGKILE
                     IGRPADLVRKHAGIDVVEADNRPEVLACLGKMGAKYEVAGERVQVFTETPREVTATLM
                     QECTQIEVVARPAGLEDVFLKLTGRRLKE"
     gene            complement(24091..25623)
                     /locus_tag="VKO45_01525"
     CDS             complement(24091..25623)
                     /locus_tag="VKO45_01525"
                     /EC_number="2.4.2.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase"
                     /protein_id="HMA04586.1"
                     /translation="MEIHDRIRELEREIGRLSRVQKILLGTDGSVTRILEVITGGRVK
                     ITTLSQEVIPAGNESAALLRVAEGEPVNHRVVSLEDAETSLPLIYAVSDTPLGRLSPE
                     FREDLMQADIPIGRILATHHIEARREILDARIVPASPEHMKVFGVFRNEPLLSRRYQI
                     IHRGEPLISIEEQFPYHAFLDERRVIVETPSRIHLGLIDMHGGIGRVDGGIGIALEEP
                     GILLDVRQSGETVARGGDRRSRETAEKAAARVLEGIRAAGGIEITLRRIYPSHVGLGS
                     GSQIALAVARGICELFGRNLPVGELARLVGRGGTSGIGTAAFEGGGFIIDGGHAFGPG
                     AEKSDFRPSGASGGVRPAPVIARHDFPPDWRIVLAIPCLPAGASGGKELAIFREACPV
                     PLEEVRETCHEILMRMLPGVVDRDLDLFGRSVNRIQELGFKRVEIGLQPAVIPRLMEE
                     MRAAGAACAGMSSFGPTVYAVTDSDGPAILRAAEKFLGEHGGGDAWISAPRNTGAAVR
                     TS"
     gene            complement(25730..26851)
                     /locus_tag="VKO45_01530"
     CDS             complement(25730..26851)
                     /locus_tag="VKO45_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04084 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HMA04587.1"
                     /translation="MYYHVILTDECNLSCTYCRGRAFQPSGPGEEAPGIDLDLPVELG
                     YEPGLLYGFLEKDPDASLIFYGGEPLLRSDLVSEMVEHAPAKRFLVQTNGLLLDRIPR
                     SVMGRIDSLLVSVDGPQAVTDGSRGEGTYRRIMENLRRVVAGGYPGEIIARMTVAEGT
                     DIASSVRFLSGNRDFSFPSIHWQLDADLSPANGSVGFADWAEGVYNPGVLALIDDWVG
                     HMEAAGEVMRWYPFIDPMEDLLTGRESPLRCGAGHANLTIMTDGSIGPCPCMAGMKDF
                     YLGHIGWSDPLALSMPSDTGSFCTDCAIRGFCGGRCLYARILRPWPEDLQRVVCGTVE
                     NLHRGLTGVLPRVRRLIREGTLEMADFSHPRYNGCEIIP"
     gene            26938..28143
                     /gene="ilvA"
                     /locus_tag="VKO45_01535"
     CDS             26938..28143
                     /gene="ilvA"
                     /locus_tag="VKO45_01535"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HMA04588.1"
                     /translation="MITLSDIRRARERIAPHIIRTPLVPSPTLSSLCGARVYLKLETM
                     QKAGSFKIRGAMNCILSCTSGIGGKGVIAASAGNHAQGVAVAAKAAGVQATVVMPVWA
                     SVSKQAATRSYGARVILSGASLEESIRYARELAGSGMTYIPAFDDPEIIAGQGTIGLE
                     ILDDLPETDLIAVPVGGGGLIAGIAVAAKGIRPGVRVLGVQAAACPSAVEAVRAGRPV
                     LVDARPTIADGIRVRQVGDLTFPFIRDLVEEIVAVEEEEIAKAMLLLLERKRVVAEGA
                     GAVTLAALASGRLKVREGSRVVLVVSGGNVDTPLLDRVIHQGLIRNGRILQAEVVLED
                     APGALAGLLGVVAGIGGNILQITHLRGGRDLPVQAVRVALDIETRDEEHGRELVKGME
                     GAGYRVERL"
     gene            28412..28627
                     /locus_tag="VKO45_01540"
     CDS             28412..28627
                     /locus_tag="VKO45_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04589.1"
                     /translation="MGQKVGSEKIKREKGYLYYLGKDGYVWAAPMKNNPGGRKKKVGT
                     ERIPKDMGYLYYIDKAGFVAKARMKNA"
     gene            complement(28725..29156)
                     /locus_tag="VKO45_01545"
     CDS             complement(28725..29156)
                     /locus_tag="VKO45_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF61 family protein"
                     /protein_id="HMA04590.1"
                     /translation="MTRRPRITEESVLTRWMGMEAARINDSVVAERKTLASLLAEEKP
                     AALTKGGARHPFDRDVLSALAAGLPEEIRKSVKIPLLFFVDVDVRDSCYLTDETAVRA
                     LKIHGDLGEGRRLTDGRLWIARAIAYAIARKYPTAVQFVLA"
     gene            complement(29153..31549)
                     /locus_tag="VKO45_01550"
     CDS             complement(29153..31549)
                     /locus_tag="VKO45_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMA04591.1"
                     /translation="MVEVLPADRFRNAYDSGRKECPATDTLQPLEEIIGQERALRALK
                     FGLEIREAGFNIYVAGIAGTGRKTAVMDFLERLAKTKPKAGDWVYVNSFTSQYEPTAI
                     LLPPGKGSEFKAEMSDFINEVKKNLPKAFESEDYATRREATVRSIDEEKAKLFAQIQA
                     SAQEQGFVIQMSPAGLLTIPVVDGKPLPEEEFVALPEETKAEIQRRMEKLNSDLRNAF
                     RQVRDLDSKGAEAVDKLNREVSLYAIGHLITDLKEKYNSLPEILKYIDAVQNDILDNL
                     DQFLGAPQQPPQVPPQLQAFFKELPFKKYEVNAIVDNSKPDGAPVIFEQNPTYQNLFG
                     KLEKEVQFGIVSTDFTMIRPGSVHKANGGYMVIPVEDLLRNPFSWDGIKTALKTGEVA
                     IEEPGERMGFITTRGLKPQPIPLSVKLVLIGTPQINQLLYTLDPDFKDLFKVRADFDV
                     VMDRNDENSMKYAAFICTLVRKFNLKHLDGTAVAKIIEYGSRLAEDQKKLSTLFASVS
                     DIIREASFYATRENAEYTTAEHVMKAIDEKTYRSNLIQQKIQEFIERDIFLIDTTGAK
                     VGQVNGLSVMGLGDFAFGTPSRVTASIGVGRDGVLDIERESAMGGPTHTKGVLILGGY
                     LAGKFAQDKPFSLSARLVFEQSYGGVDGDSASSTELYAILSALSGLPIRQTLAVTGSV
                     NQKGEVQAIGGVNEKLEGFYEVCKAKGLTGEQGAMIPASNVQNLMLKEEMVEAAKAGK
                     FRIYPVKTIDEGIQVLTGVPAGERREDGTFEEGTVNYLVDKRLREMAEKLREFQPAGR
                     "
     gene            31784..32173
                     /locus_tag="VKO45_01555"
     CDS             31784..32173
                     /locus_tag="VKO45_01555"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HMA04592.1"
                     /translation="MKVKKNPDMIRTELREAENDLEIAGKSFKGDNDKWAVKQSHRSL
                     LHVFRALLLMKGYEAKGVDCLKYAMDELYVRKGVFSADILEDFEYGREIAKGNDCLCV
                     YDRNEIEEILAVNRVLLEQATEMAKKA"
     gene            complement(32207..32590)
                     /locus_tag="VKO45_01560"
     CDS             complement(32207..32590)
                     /locus_tag="VKO45_01560"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMA04593.1"
                     /translation="MPCIQCGWCCLQYRGFHWAKVPDLLRWHGEGRRDIMQYVSAGRN
                     PDGTMKTAADLSDAELGGMEPATGWTDPTTGTSLEACPFLVKADDSRYLCSIHPTKPA
                     VCRDTNTWEWGLGRYGPWGCPASPP"
BASE COUNT         5862 a        10099 c        10992 g         5782 t
ORIGIN      
        1 aaaaaggagc acgaagtaga tcagggcggg ggtaacaggc tcgtctttta cctcacggaa
       61 tgcggtctca ggactcccga caaaggccgt gattttcctg acgaggtcca ggcccatgga
      121 agagggatcg gcatcgaata ccaaaaatgc ttccccggaa tttctgaaca ccgggttggg
      181 aacgataaaa agtgagaagg ggatggaggg gctctagaag tcgcccatgt cgccgcccat
      241 gccggggggc attccgccgg gaggcatgcc gccgggcccg ccggggggtg cgcccgcaac
      301 tcttgaggcg gcgatgacgt cgtcgatccg gaggatcatg accgctgcct cggccgccga
      361 ggcgacggcc tgggtcttca cccggagggg ctcgacgacg ccggccttgt acatgtcgat
      421 gggtgcgccc ttgaagacat cgaggccgta gttcttcctc ttcttgtcat gctctgcccg
      481 gagggcgacg agcatgtcga tggggtcgag gcccgcgttc tcggcaaggg tcctcgggat
      541 gatctcgagg gcgtttgcga aggcctcgat ggccagctgc gcacgcccgc cgacggatgc
      601 cgcgtagtca cggagccgga gggagagctc gatctcggca gccccgccgc cggccacgag
      661 cttcttgtcc tccatgacca cggagaccac gctgatggca tcgacgaggg cccgctcgag
      721 ctcgtccacg acatgctcgg tcccgccccg cacgatgata ctgacggcct tcgggttcac
      781 gcacttctcg acgaagatca tctcctcgcc cccgaccttt ctctcctcca cgaggccggc
      841 tttgccgagc tcgctcttcg agattgcgtc gatggacgag atgaccgatg cgccggtcgc
      901 ccgggcgagc ttctccatgt cgctcttctt gacccggcgg agggcgagga tgtttgcctt
      961 cgcgaggtag tgctgggcga tgtcgtcgat acccttctgg cagatgacga cgtttgcgcc
     1021 gctcgccacg accttgtcga cgatggactt gatcatccgc tcctcttcat cgaggaacgc
     1081 ctggagctgg tcggggctcg tgatgttgat ctccgcgtcc acctcggtct tcttgaactc
     1141 gatggaggcg ttcaggagga ggatcttcgc gttctccacc ttcttcggca tgccggggtg
     1201 cacgcgctcc ttgtcgatga gcaccccctc gacgatcatc gagtcgtccg aggacccgcc
     1261 gaccttcttc tcgatcttga cgttgtcctt gtccacggtc ccgtcctcgt cgacgaccat
     1321 catggccgcc ttgaccacga gctcgcaaag cttatctttc gaggcctcgg cgccctttcc
     1381 cgtcatcgcg gtctcggcga tcttgaagag catctccttg tcggtgggct tcaccgtgat
     1441 ggcgatgtcg tttaagatct cccgggcctt ctcggcggcg agccggtagc cctgggcgat
     1501 gaccgtcggg tggacgtcct gatccaggag ctcctcggcg cgcttcagga gctcgcctgc
     1561 cacgaccacg gcggtggtgg tcccgtcgcc gacctcgtca tcctgggtct ttgcgacctc
     1621 caccatcatc ttcgccgccg ggtgctcgat gtccatctcc ttcaggatgg tgacaccgtc
     1681 gttcgtgatc accacgtcgc cgatggtgtc cacgagcatc ttgtccatcc ccttggggcc
     1741 gagggtggtc cgtaccgcgc ctgcgactgc ctttgccgcc gcgatgttgg aaccctgggc
     1801 gtcgcggcca cgggtccgcg agctgccctc cttgaggatg agaatgggct gtcctgcgag
     1861 ttgtgacatg attctctcac cgtattttgc ggtagaataa ttggtttgtg gttctatata
     1921 aacgaagtgg atccctccgg ggatcccacg gagggaagac ctctcgaaaa gtgaatatca
     1981 cgccgggggt tcccggatga gctccatgag ctcggagccg tcctccaggg cgtggaggcg
     2041 ctcctcgccg atgacgaggg tgtccccgat ggtcttctgc cgccggtatc cgaagaggac
     2101 acagagggcc cgggccctcg cgatccggga tatgctcccg atcagggggg cccgcttctc
     2161 cacgtgctgg gcggtaccgt agccggtgag gatggtttcg tcgtcgtacc gggcaagggc
     2221 ctcgaagggg gcgcggcgca gcaggtggat ctccatcccg agccgctcga gatcggggag
     2281 cacctgcccc ctggcgggct catcggcccg ggcgaatcgg gattcgtggg agagaaggtc
     2341 gacgggcttc gtgatggcgg tgtcgaaaac ctcctcgata cggacggcga tctccatcgt
     2401 ggtccccatc ccgttctcgt acttgctgat ggtccggcgc gaaacaccga ggaggtgggc
     2461 gaggtctccc agggaaagct ccctctcctc cctgagggcc ctgaggagct tttcatcgat
     2521 gttcacgtag agcccgccgg gcgagacata gatgaggggc gggatcccct ccatgaggaa
     2581 atccgagagg gtggggaggg agagggcata gatcccgtac cggacgtaca ccgccccccg
     2641 ctggaggtcc gcgtcccggg tacgctcccc cacgatgagg ggggctccgc ccaggtagta
     2701 ggagacgagg tccaggtccc gggcgatgtc ctcggagacc ccgtcgatgt gggagatggc
     2761 cttcaccacc atgaggacct cccggtcgcc ggcgatgagg tcaaaggagc ggggccgctg
     2821 gctgcaccgg tccgagacct ggaaccccgc ctcgacgagc aggctcctca cccgctggag
     2881 aaggacctcc tgggtcatgg tgcctcaaga agccatattg cagggtgtga taataaaata
     2941 tcgggcatgt ggatcggcat cgatgacacg gactcccccc gggggatgtg caccacgtac
     3001 ctgggggccg tgctcatccg gaggctcctt tcccaacgtt tcgctctcca tgaggcaagg
     3061 ctcatccggc tcaacccgaa cgtcccctgg aagacgaggg ggaacgccgc catctccctc
     3121 gaggttgggg gggatcccga ggttgccttc gggatggcct gccggtgcgt cgatgagatg
     3181 gcagaccttc ccgccgaggg gaccgagccg ggggtcgcgg tatcggagac gaagccgccg
     3241 gccggatttt atgagaaggc cctccgttcc ttctgcgaac gcggggaggc catcgaggtt
     3301 ctcgaggagg ccggggcgat gtaccgggga tggaagggag gccgggggct catcggggcc
     3361 acggcggcca tcgcgagcac ccttcccgac tccacctggg agctgcttgt ctaccgaaag
     3421 ccggacctcc agggaacccc gaggaacatc gaccggggat ccttcttcct cgccgatgcc
     3481 tccaccgcgc cggccacctg ggacacggtg gaccgggaga acggtatcgt ggtctgttcc
     3541 ccccacacgc cggacccggt cctcttcggg atccggggcc gaagcccctt ctctgtcgcc
     3601 ctcgcccgga catttatccg gaccgagccc gcgggtatgg agcaggtgta tctcacgaat
     3661 cagggaaccg atggccacct cgaagagggc gtagtcgggg gattgtccga ggggcactcg
     3721 tacagggtcc agggaatcgt atcgggaagg gcggcgacgg gaaggggggg acatgtctcg
     3781 gttcccatcg gtgacggggg gttgtccctg aggtgcatgg cctacgaacc gaccaagggg
     3841 ttcagggaca tggtgagggc cctcgtaccg ggcgacgagg tggaggcctg tggaagcttc
     3901 aaggggggtt cgctgaacct ggagaagatc cggctcatcg agcccgcgca ggatctggtg
     3961 atgagatccc ccctctgccc tgcctgcggg gtgagaatga cctccgcggg ccgcgggaag
     4021 gggtacaagt gccggcggtg cggtgagagg gcagcagggg gagaggttca ggagagagcg
     4081 agaggaatcg cggcaggatg gtacgaggta cccccctctg cccggcgtca tctcgcgatg
     4141 ccccttgcac ggggaattcg gcacagataa gagggggcag tgaaatagtc gtgaaagagg
     4201 agagtgcatg gaaggcgacg cggtgaagca ggtgaggatc cggcccggca tgaccgccgg
     4261 ggaactcgcg agcgagctcg aggcatccgg ggccttcaac gggggatccc ttgcaaaggc
     4321 atgcaggatc acggaggcga tgttccggga cgggaaggcc acgaccttcc tgggtctcgc
     4381 cggggccatg gttcccgcgg ggatgggtgg gatcgtggcc gaccttatcg acagggggta
     4441 tgtcgacgtt ctcgtctcta ccggggcgaa cctcgtgcat gacatggtcg aggccatcgg
     4501 gtgccggcac tatcacgggt cggcggagtg ctcggacaac gaactccgga ggaaggggat
     4561 caaccgcatc tacgatgtct atgtctccga cgacgccttc acccagttcg aggacttcat
     4621 ccagggagtc ttcggggggc tcagcgacgg gacggtcatc tccacctcgg agctcctggg
     4681 catggtcggg aagaagctcc cttcggggat ccttgcgacc gccgcgaaaa agggggtccc
     4741 ggtctactgc cccgccgtcc aggactcggt tttcggcctc cagttttccc tcttttccca
     4801 gacccacaag gtgacggtgg acgccttccg ggacatgaag ggattcctgg accggtgctt
     4861 cacctccgag agcgcggggg cccttctcat cgggggaggg gtaccgaaaa acttcatctt
     4921 ccaggggatg ctcctcgccc cccacgggtt cacgtacgcc gtccagctga ccggggaccg
     4981 gccggatctc gggggcctgt cgggcgccac cctggacgag gcccggtcct gggggaagct
     5041 cacccccgag gcgcagggcg tgaccgtgta cggggacgcg accatcactc tcccgttcct
     5101 tgctgccgcg gtactggaga ggctctcccg tggctgagct gatcctctct ctcgacgtta
     5161 ccgaccgggc acgggccgtc gatattgccc gggcctgcgc cccccatctc gatgccatca
     5221 agatcgggta ccccctcgtc ctgtcggccg ggatatccat tgcaaaggac ctcctccccc
     5281 tcgccctccc cctcatcgcc gatttcaagg tcgcggatat cccggacacc aaccggacca
     5341 tctgcaccct cgccttcgat gcggggtttt caggagtcat cgcccatgcc ttccccgggg
     5401 aggatgctct ctccgcctgc gtggaggtgg cccatgcccg cgggggggcc tgctacgccg
     5461 tcgccgagat gagccacccg ggggccgcgg ccttcttcca tggcggggtc gcggagcggc
     5521 tggccgcgct tgccgttcgg tgcggtgccg acgggatcat cgccccggcg acccggcccg
     5581 ggagggtcgc gcggctcagg gaaatcgcgg gttccctgaa gatcctctcc ccgggtgtag
     5641 gggcgcaggg aggggagctg gaggcggtaa aggatctcgt tgacgggatc atcgtcggga
     5701 ggtccatctt cgcggccccg gacccgaaag ccgcggcaga ggatttctcc aggcgctgcc
     5761 gatgatgagg cggccgatct gaccgtgatg aaccctgtga gtcagctggg gcagcctggg
     5821 aagggtcgac cgacctgcca ccgccggccg tttttcatcg atgtccgcga acttgcgcaa
     5881 aataaaattt tcgcgcaaaa atattttaag atcgcctcac catggatccg gtatgcaatg
     5941 gaatccggag cagcgcaagc ttatagaaaa ataccattcc atccaggata tcccgcccgg
     6001 ggaacggcgt tacaagtgtt tcacctgcca caccatcgtg gacacgtcgc catgcccgag
     6061 gtgcggcgag gccaggctcc agatcctctg cccgctggac catgcccagt gctcccatga
     6121 gatcatcgcc cggatcgagt actgccccat gtgcgggaac cccgtctgcc ccgactgcgg
     6181 gtcgcatgac gtggtgcaga tcagccgggt gaccggctac ctccaggatg tctcgggctg
     6241 gaatgccggg aagcagcagg aactgaagga ccgggtgcgc tataccctga gctgatccct
     6301 gtcctttcat tctcttccga aaatagaggt tgccggatcc ccggtacggg aatccgttca
     6361 cttcttgtta ttcccgtttc cctggccctt ctccttgtcc ttgttgccct ttccgttccc
     6421 gttcccgttc ccctggtttc cctgtccctg ggtgtcggcc tgatcgtcct cgtcgtcaga
     6481 ggcggacttc ttgccgttgt ttcccttgat attcttggaa ttaccggtgg cgcagttctc
     6541 ggtgcaggag ccatcctggt tccgttcctg cttcttgagc tggccgcggt tctggttctg
     6601 gtccgggctc acggttggag tggcggttgg ggaggtcgag gtctcatctg ttccctcccc
     6661 gacctcgtcc aggggcgcaa cggtgtccag gatatcagag aagaacgcat cgaggcccgt
     6721 cttcttctct ccgatcaccc ggatggtgtc gctcccgatg accttcacgg tctccccgcc
     6781 cttcgtgacg gtcccgttgc agacgatctc gacggtctca ccggcaacta catccttccc
     6841 gaggctctgc ctccggaact tggccatgag ggttccgccg gccttctgat tcatcttgac
     6901 cttcgtcgac ggcgcgcctc cacaggtgat ggagctgata tccctgaggg tgatcccttc
     6961 ctgtgaggta tcaccggcga gccggataaa cgccgtgaag acgccacggc tcttcaggtt
     7021 cagggtctcg ggcttgatgg tgacatccag cttcaccgtg gggctggtct caccggaggt
     7081 gcataccggc tcagagccgg cctgggcggg ggaggtgatc ttcgcgcagt tcttcagctc
     7141 accggcgatc gcatcctcgg caagggtcat ggtcaggttg agggtggcag tggccccgtt
     7201 accggcgagg gaatcgatgg tccacgtatt ggtcgcatcg tcgaaggccg agccggcgct
     7261 cgcctcggcc ctcaccaggg tgaaatcacc gagcgctgag ggatcttcgg tgaccacgat
     7321 atccgtaacc ccggcggtcc cctggtttgt cagggtgata ttccagatga cctggtcccc
     7381 cgcaaagatc gggcccgtgt tgaagagggt cttcgtgagt tcgaggtctg ccgcctcggc
     7441 cgtgatggtg gcggaggcgc atacgggcgt ttccgatatc tcctcgctgg gagcggtgat
     7501 actgacacag ttcttgatct ctcccggagc cgcatcctcg gcaagggtca tggtcaggtt
     7561 gagggttgcc gaggcgccgt caccggtgag cggaggaacg gtccaggtgt gagcgttctg
     7621 atcataggtc gtgccatcgc tcgcctccgc gaggtcgagg gtgacatccc tgagaagtga
     7681 catgtcgtct gtgactgtga cctcaggggc cgcatccggt ccgctgttcg tcagggtgat
     7741 attccagatg atctggtccc cggcggttat cgatccttcg ttatggaggg tcttcgtgag
     7801 ttgaaggttg gccgctgcct gggccgcaga taccgcgcca caccataggg ccatggcaat
     7861 aaggaggagt gctcccatct ttgcatattg agtcatatca gtctccgggc gaatcctgtt
     7921 cttggttctc cagggattcc ctgatcccgg atttcatggg ggggcccttt aaggccggcg
     7981 accgtcccac ccggtccgct cggttcgggg tggcccggga gggaggggat tcgtccagca
     8041 tggttcgaaa agaaaaaaca gccgcgtttc gggcttgatt ccggtgccgg aattccgggg
     8101 caggaacggg acttttccct gcatccccgg gacgcgatcc ccgcacccgc gcagccgggg
     8161 cggaaccttc tttttcatcc cggcggagat ctctttgatc cccgatgagg tacttcttca
     8221 ggggcgggac cctctttgtc cggggggact tccaggccgc gagcaccggc gtgaatggcg
     8281 gtcttcggag ggtcaccacc ctcctcaacc acacggttgg ccgggacttc ccccacgagg
     8341 aacccctccg ttcgctggag gatctcgtgg caggcatggg gttctcccgg gacttcttcg
     8401 ggctcctcac cgcggtcgga atccggcaca cctgcatcct ccagtacgac ttcatcaccc
     8461 tgtttgtcac ggccgggatc accggtcccg ttgcggagac ccccgcgggc accatcaaca
     8521 tcatcatcca ctcggccgag ggattctccg acggggccct cctggaggcc ctcctcgtct
     8581 cgaccatggg gaaggccgat gccttgagag ccctcggcca tcccttctcg gggaccccca
     8641 ccgatgccgt ggtcgccgcc tgcgaggggc cggcacgcca cgagtacgcg ggaaccgcca
     8701 ccgaaggggg gaggcgcatc tacgaggcag ccttccacgg ggtagaggag gccctggcca
     8761 ggcacgagcg cagggttgag agggatgccc cgtcgttgtt catctattcc cggtacggcg
     8821 gggatcactg ggtggagtgg aggccggaag gctgtcccta ctatccctgc catttcgagg
     8881 gacagcggtg cgactactgt tactgtcccc tctacccctg cctggatccc accctcggcc
     8941 agtgggtgga gagcaagaac ggcggacggg tctggaactg cagcgggtgc acgttgctcc
     9001 acatcccgaa ggtcacggag tacctgaaga agaatcccga ggcgacgctc cgggagctga
     9061 agagggcggc cggctgaagg ccgacaccca ggaaaaatta attctcggga attccgaggg
     9121 cggagaggag ttccttgagg ggaataccat gggcctgggc cgcctcccgg atggtctcac
     9181 cccgggccac ggcgcacccg aggcacccca tcccgaattt gaagaggaca ttggccgcgt
     9241 cgggctttgc ctgcaggacc tccataatgg tgctgtcagc cgtgattgcc atgcccatgg
     9301 tgttggtcat cctgctactt aaccctttag aaaacaggga cggaggggac aaattccatg
     9361 ggagggcacc tcattttcac ccctcccccc tcatccttta aggcaggtcg gtgaacctta
     9421 agagtggaga tgtctatgga cgtatcagat gcggttgaaa gaatctccgg taagatggaa
     9481 gcaaagggaa tcgccgttga ccggccgagg atcgaatcga agatacggcg tcttgttgag
     9541 gagttcgggg ttcagctcgc cgaggccgag cggacggtgt ggaacgagat cgcccgggag
     9601 cactccctcc cctcccctgg tgggagggct caggaacggg agatccgggg gctgtcggcc
     9661 ggcgagtggg tgacggtgca gggcaaggtg gttgccctca cctccccgcc caccccggcc
     9721 attgcgcagg cggggatcct cgcggatccc tccggcgcca tccggttcac ggtctgggcc
     9781 cgggcgaagg cccagcccct gtccgccggg aaatggtacc ggatggaatc ggccgtcgtg
     9841 gatgagttca ggggcgtgga aaagctgaac attcacagcg ggaccaccat caccgaggtg
     9901 gagaacgagg cccccctcat cccgaaggtt gtcccgatcc gggagctcca gcccggggtg
     9961 ggttccgtca gggtgaaggt ggtccaggag tgggaggcga gccacgagag gatgctccag
    10021 acgggtctcc tcggagacga gacggggacc atcaaattca ccatctggcg cgaggaggga
    10081 aaggagaagc tcactccggg gaaagtgtac tccatctact atgccctcgt ggacgagttc
    10141 aacgggaggc tccagctgaa cctctccccg gccatctacc tccccgagga gggggatatt
    10201 caggtgtcgg agggggagac gacacggggg gtcatcgtcc acctggctcc cggttcgggg
    10261 ctcatcaaaa ggtgcccggt cgaagggtgc aaccgggtcc tctcccggca gaattactgc
    10321 ccggtccacg agatccagaa gaccttccgg tacgacctcc gcatcaaggg cgtccttgat
    10381 gacgggatcc ggacgaggaa catcctcgtc cagaaggaga tcgtcgaggc cctctcgggc
    10441 atgaagctcg aggaggcggt ggcccttgcc gagtcgagcc cccttggaat ggacgaggtc
    10501 ttctaccgga tccgtgacgc ggtcctcggc cgctacttcc aggcgagagg taccgagatg
    10561 gacgggagaa ttctcgtcaa ggaatgccgg aagctggcat acgacccgtc ggacctggct
    10621 gccctcctga accgggccgg gggcgaggcg aaatgatgaa cgggcggaag gcgggcggac
    10681 cccgcccggg gcagaccttc gaccgggaac cagcccggcg ggtttttgcc ggtgaactca
    10741 gggaggtgag gctccagttc cgggaggggg aggacgagaa gagcccgagc ttcgtcctcc
    10801 ttcccacggg atcacgctgc aaccggatat ttctcgtcgg caccctgacc gagaaggacc
    10861 ggcgcggcga ccagaacgtc ttctaccatg cccggatctc ggacccgacc gggaccttct
    10921 tcctcatggc cgggagctac cagcccgagg cgatgcagca gatcgcccgg atcgagccgc
    10981 ctgccttcgt ggcggtcatc gggaagccga atgtctttga atcccagacc gggtcgttcc
    11041 tcgtctcggt gagagcggag tcggtgaccg cggtggaccg ggagacgaga gacctctggg
    11101 tgctggacgc cgcccgggcc accctccagc ggctcgatga gctggggaac accgaggact
    11161 cgaaacgggc ccaggaacag taccacccgg atcccgcggt gtaccggaag atggtgtacg
    11221 atgccctcgc ccagctgaaa atttaatcac cacctttttt ctcccgcaat tcttatctgg
    11281 gtgcagcccc atcggtcccg gcatggaaaa aaacctcctc gtgccccggg tgagggccgg
    11341 ggccgcccgg gcagtccggg ccctccccct catccccctg ttcattctct cgggttgcct
    11401 cgtccccggt gcacggacgg ggacacccgt gctcgaggta tcctccgccg cctttgcccc
    11461 tggtggagag atcccggtga tgtacacctg cgacggggcg aatgtctcgc cccctctcag
    11521 ctggagcgcc cccccgcagg gcacggagtc cattgcgatc ctggtcaccg acccggacgc
    11581 acccggcggg accttcgtcc actgggtggc ctggaacatc ccgcccggca cccgggagat
    11641 cccttccggt agcgctggga aaccggtcct ccccaccggc tcggtccagg gcacgagtga
    11701 cttcgggcgg cagggctacg gcggaccctg cccgccggga gggaagcccc accactacca
    11761 cttcgtggtc tatgccctcg actccgccct cactctccca gggaagaggg acgggcggac
    11821 gctcgaatcc tccgtgaggg ggcacatcct tgcccagggg gaaaccatcg ggatatatcg
    11881 caggatatag gacttcaaaa aaagggagag ggggaatctc ccggattgac caagggagat
    11941 cggtccgctc ttttcaccta cccttttctg cgcttcagga tcagggtgag ggcggccatc
    12001 gccggggcga ggaaggcaag ccatgctccc gtcggtgcag gtgtgggagg cggggaggtg
    12061 gtacccgttg ccgggggaag ggtcgtggcc tcgggggtcg gggtcccgac ggtggttacc
    12121 gggcgcgtgg tggttggcgg gattgtctgc cggacggtcg tggcggtggt ctgcgtcgtg
    12181 acgggggtgg tcaccggtgt gggggtcgtc tgaacggcta tttttttccc gatggtgaag
    12241 gtaatgatgt tggagtccgg tgcctggttt tcaaaggagg ccgggtctct ccacttcgcc
    12301 tggacgctgt agctcccggc gccgaggctc ccgagggaga tggcccccgg ccttcccggg
    12361 tcatcggtat agaagacctg gcggctcacg tgcatcgcag agaaatcccg gcccaggatg
    12421 gtggtgagct gggcaccacc gggagcggtc aggacgagat cgacggtcgc cgggaagagg
    12481 gacccggcat ggtacgtgga cccgacgtcc acggaggtga tcttgaaggc gatgggggtg
    12541 tcctcgggaa tggtaagccc cgcgatggag tccacgtggt tcgggcttgc gagcacggca
    12601 tcgatggaaa ccgagggaag ctggaccacg accctgccca tggccccgac cgccggattg
    12661 aaggcaatat agaccccccg gagatccccg aattcctccg ggataaggct gaagctggta
    12721 tcgtcggaca ccgcgacctc ccggaggagg gccttcgtcg gatcatcatc ctggtacttc
    12781 cggagggagg cgaccgggtt cgccccggta cgaagcgcgg tgatgtcgag attctgttcg
    12841 tagatgaaga tcatgtcccc gttctggatg gtctggatgc tggtccgggc ggccgaggga
    12901 accgcgagga ttgcccagag gagcaccacg aggactcccc cggttacgac ggcccgggag
    12961 atctgttccc gggacgatgg agaacctgtc ataagacctc agccccagtg agggtatccc
    13021 cgggatataa gcccttccgt gcaactgggg ccagggtgcc cgatgccgga tgacccggat
    13081 gagaatcgca ggggcggccg tgtttcctta accatcctcc ccgatctcga cagttcctga
    13141 gtccaggggt gcggacttcc gatccctctc ctccaccctc tcccggagga acctgacagt
    13201 cctttcgagt tcggcgacct tccgggagag gctctccttc cggtgggtcc ggtggagcgt
    13261 catcacaggc tccccggcca gataagcccg ttccaggtcc ttttccacct gccccgcggt
    13321 ctccccccgg ggagtacgga tggagcccat cagctcccgg acgacggtgg ccggtaggac
    13381 cgtacccatc cgatcggcga agaaccgccg ggcgctccgg agggtaaggg tccttctccc
    13441 cgtcacccgg tccaccctct ccagccccgc cttcgagagg gcgaggtccc agacctgctc
    13501 gaaggcccgg gggcctatgg gaaaaagaag gtcctccccc gcggtcccac ccctcgcccg
    13561 ggcggaggcg aggaaccggt ccctcacgat gaggtatacc cggatggccc ccaccgcctc
    13621 gcgggagatg aaggcgaccg tcggtgcctc cgtcttcccg gttcggatca ggaacctggc
    13681 cggccgggca tcgaggtcga gatccccgag ccggatggag agaacctccc cgaggtgcgc
    13741 gccgctgctc accagggtgt agacgatggc cctcccgcgg gtatcgaggt gttccatgag
    13801 cctcagaagc tttcccgggg tcagggaatc gacagcggca ggtggccggt gactcccccg
    13861 tgcccgcggc agccgccttg cgtcgccggg gggaagggcg attcccgcct ctgcaagaag
    13921 ggtccggatc gcgctccggt acccgaggca ggtcctccgg gaccggcagg ccgcctccag
    13981 ggcccggaga tcggccgcga ggtcgcgcct gccgttcctc aggtcgttca cgtacttggc
    14041 gacccaggga tcgaggaggg cgggagaggc ccccttcagc cgggcgccgt acacaaggga
    14101 aaggaatttt atccatgcac tcccatacat cgaacgggta gatttcgccc ggtacctgtt
    14161 caggaaatcc acgaacatca gggcatcccg ggtaagaata tgccccataa tcatttaatg
    14221 gtatggaatt gcgaaggttg atacgagttt aaatctcatc aagcgaaccg gctccgtccg
    14281 gtcggaccgg agacaaaaga ggagttacca tgaagtcaag gagagagagg gaagcatcga
    14341 gactgagaga gaagggggtc cggccccaga ccgcaccccc ggctgagatg agggacccga
    14401 aggcggtgga ccacctgatc cgggcccttt ccgacaagga tgagacggtg cggtcccagg
    14461 cagcccgggc cctgggcgag atgcgggacc cgaagggagt ggaccacctg atccgggccc
    14521 tgaaggacag gcacaagctt gtccgggccg aggccgccga ggccctcggg agaatcgggg
    14581 atcccctggc cgtggaccac ctggtccggg ccctgaagga cgagaacgcc cgggtacggg
    14641 gagcatcggc ccgggctctc ggggagatcg gggacctcca ggccgtgccc gccatcatcg
    14701 gcaccctgaa ggaccgggac gaggatgtcc ggggcgaggc cgcccgggca ctggggaaga
    14761 tgggggacga gcgggcgctc cagcccctca tcgaggccct gagggacatc gacacctatg
    14821 tccggtgcta tgcgaccatc gccctcggga ccatcgggac cccggccctt gactccctca
    14881 tccaggccct caagagcgag tacgacgacg tccgggagag ggcggcgaac accctcgtcg
    14941 ggatgaagga ccgggccgtc gagtccctca tcggggccct cagggacgag gactgggcca
    15001 tccggaagga ggcccggcgg atcctcgccg ccatcaaggc ccccgccgtc gatcccctga
    15061 tcaaggccct cagggacgag gactgggacg tgcagcggca ggtggcacag gtcctcgagg
    15121 agatcgggga cgaccgggcg gtggagggcc tcgtcgatgt gctcaagaag gccccctggg
    15181 ccgcccagaa ggagatcatc cgggtcctca cgagcttcgg ctggagcccc ctctctgatg
    15241 aggaacgggc ctacttcctc atctccaagg gttcctggac cgagctcgag cagatcggcg
    15301 atgccgccgt gcccgccctt gcgaatgccc tcagggacga ggacaggtac gtgcggaggc
    15361 aggcggcaag ggttctcgga gggctcgggg atcgggccgc cgtcgagccc cttatcgagg
    15421 ccctggaggg ggaggacgcc tatctccgtt ccatggccgc ggagtccctg gggagaatcc
    15481 gggacaagac cgcggtccct gccctcctca aggccctgtc cgccgatgat tcctgggtga
    15541 agggatacgc ggtcgaggcc ctggggatga tcgcagacgg ccgcgccgtc gagtccgtca
    15601 tcggggtcat gaagtcgggc ggcgatggga tccggcgggc gggggcccgg gccctggcgg
    15661 ggatggggaa gccggccatt aagcccctca tccaggtcct ctccggtgac gaccgggaga
    15721 tgaaggaaag cgccgcctgg gtcctcgcca tggcagggga gctctcggtg ccgcccctca
    15781 tcgattccct caagcgcgag ggggacgacc accgggcgga tgccgaggag gtcctcttcg
    15841 agatcggcga gccggccgtc gatcccctca tcgccgccct cggcgactcc cacgagcccg
    15901 cgcgtcgctc ggcggcaagg gtgctcggca ggttcgggga ccggcgggcg gtggagccgc
    15961 tcatcagggc catcggggac tcctcggccg gcgtccggaa ggaggcgacc atcgccctcg
    16021 gcagaatcgg ggagccctat gcggtgaccc cgctcgtcgg gctcctcgcc gatgacgagc
    16081 tcgaggtgca gcgcgaggcg gtggtggccc tcgggaaggt acgggatatc cgggcccttg
    16141 cccccctcat cgggagcctg aggaatcccg agctccggga gaacgtgacc gaggccctct
    16201 cggcgatgag cgacgagcgg gccatcgtgc ccctcatcga ggccctgaag gacgagagtg
    16261 tctttgtccg gatgggggcc gcggatgtcc tcgtccacat cggggaaccc gcggtggacc
    16321 ccctcatcga ggccctctcc catgacgatg cgggtgtcag gtacgtggcc acctggaccc
    16381 tggggaagat cggcgaccgg cgggcggtgg agccgcttat cgcggccatg aaggaccggg
    16441 acaagggcgt gaagaagggc gcgtcggtcg ccctcgggga catcggggac gggcgggcgg
    16501 tggagcccct catccaggcc ctcaaggacg aggacgagtc catccagcgg gccgcacggg
    16561 aatcgctgga gcggatcatt tcaaagaagg actgacgtcc tttttctcac cctccctttc
    16621 ggtcgggtct cgaagaagga ctaaggggat ccccctcccc tgctctttcc tttttaattt
    16681 ttagttgcct gaaccgacgg gaatcggttt caccaatatc aaatggttca tattccgccg
    16741 gcacgggcaa tcccccatgt ttccggaatc acccgatatg ttagttcatg gaatgatcgg
    16801 cccatcacaa ccgggagacg ccgcagcggc gggggtgagc cggagcgaac ccccaggagc
    16861 gtccattggg cagcgatgaa gttcggatca tgagatagtt tgagaatctt gatgtttttt
    16921 cttcaggatt tgcgccataa gggcaccggc cgctcggtca cgaagagaga ttttccgtcc
    16981 ccctcctctc cctgaccttt ccgaggacct cccagatgct gtcgcgctcg aggaggatcc
    17041 agtacctctt cagttcttct tcggcgtggc ggtaatcggg gaagaactcc gcgaccctcc
    17101 tccagtacct ccgggaatgg ttcggttcca ggagatgggc gagctcgtgg actaccaggt
    17161 agtcccggat gtcatcgggg agggcgagga cccggaggtt gacgctgatc ctcccggcag
    17221 gggagcagct cgcccaccgg ctccgctgct cccggacgga gaggcccgtg aacccgaccc
    17281 tcatccggat ggcatgggcc tccaggcggg ggagaagcga accccggagc cggtccttca
    17341 ggaaggcctt gagatccccc ggggtcgtgt aggtgatccc cccctcctcc gccctgatcc
    17401 cgcaggaggg gccgttctcg agccggaaaa acctcccgcc gaggaggatc cgtccctcct
    17461 gcccctcccg gtcccgggag atctcccgga tctccctcct cttccgctcg atccaggcga
    17521 ggttgggctc gatgagggag gggatgtggg tgcccggcgg ggcggtgacc tggacggtac
    17581 cggaggggag gacccggatg gtcgggcgga ggacatccgc ctcccggatt tccaggtcga
    17641 ggccgtccgg aggcacgggg gtgtcaccgg gtaagggagg cgatgatggc atcccggtga
    17701 ggcgtgatcc tcgatgcgac gagggcgacg taccttccct ggaacctcgc ccccgcgagc
    17761 tcgttttccg tcggccagcg ctccccgccc gacccggcga tggtggaggc cccgtagggg
    17821 gagcagccgg tgatctcgtc cagccggctc tggccggcga agaggtaggg gagcccgacg
    17881 acgatcatcc cgtggtggag gagggtcgtg tggaaggaga ggatggtgga ctcctggccg
    17941 ccgtgctggg tcgccgagga ggtgaagacc gagcccacct tcccggcgag ggccccctgg
    18001 acccagaggg atccggtcgc gtccaggaac tggcgcatct gcccgcacat attcccgtac
    18061 cgggtcgggg tcccgaagac gatggcatcg taattcccga gctcacccct ttcgcagacg
    18121 gggacatggg agaaggcctt ctgggccttg aacgccccgg ttttctcgag cacctccgga
    18181 ggaagagtct cgggtaccct ccgcatctcc accgagaccc cgtccacccc cgaggccccg
    18241 gccgcaatgg cctccgcgag ccggtggaca tggccgagca tggaataata gacaacgagg
    18301 atcttcatgg caggctcaca ctctcccggg aatcaacgac agtgtgtctc gtcccgggat
    18361 gaaaagggtt tctcggtccg ttttactccg gctggccccc gggggcggaa tgcgggttcc
    18421 cctccgcccg gccaggccat accttcattc cctcgctgta ccgggcggca aaggacccga
    18481 cgagggaggt cgcgaccacg aggatggcca ccgtggccga gatggccgcg gaaccgaaga
    18541 cggcggcgag ggcgatggag aactcccccc gcccgagggt gttcgccccg atctcgatcc
    18601 ccgcggggac cgagccgtgg agccgccacc cgacgaggat ccccgaggcc atcttggacc
    18661 cgagggcgag gaggcagagg accgaggcga cgagggggga gaaccctccc gaaaagtcga
    18721 tggtgatccc gaagaagacg aagaagagga cgaggaacac ctcccggaag ggctgggaga
    18781 gccggtcgag ggcagaagcg ctcgtcgcgg cgagagagga gccgaggatg atggccacga
    18841 gggcatcggg gatcttcagg agcttcgcga ggtaggtgga ggagatgacc gccgtgaagg
    18901 tgaagaggga agggaggtcg tcggtccggg cgaggagcgc atggatgggc cgccggaggg
    18961 cacggacgac gaggacggtg gccgcgagga ccgcgacggt gaggaggatg aacatcaccg
    19021 ggttctccga ctgggcggaa aagacggaga tgaggaggat caggagcaca tcctcaaaga
    19081 ccatcatcca gacgatggtc tcggcctcgg ggaggatgag cctccggttc tcgacaaggg
    19141 aggcgatggc catcgcggag ctcgagatgt agaaggccga ggcgaggatg aaggactcga
    19201 gggggggaaa cccgaggagg aaggcggcgg caagaccgac cgcgaggttc acggagaggt
    19261 cgatcccccc ggagaggagg agggagcgcc cccgttcccg gagggacttc gggcgcatct
    19321 ccaggccgac gtagaagagg aggaagatga gcccgaggga ggagaggtac tgggagtagg
    19381 cgtcggaggg aaccagggag aggccgcttt tcccgatggc gattccggcg aggatgtaga
    19441 aggggatggg gggcagggag taccgcttgg agaggagggc gaggatgagg cagaggaaga
    19501 gggcgagggc gatctcttcc atcagttccc caggatctgg tgctcgaact cttccagctg
    19561 gtcggtgtcc ccgatggcga ccacgatgtc cccgttctcg aaggtgaagg acgggagggg
    19621 gttgatgatg ttcttccccg cgcgggagac ggcgacgacc gtcgcaccgg tcttggtccg
    19681 cacctggagc tcggcgatgg accggccggc gaggttcttc cggatgacgt aggtgtggac
    19741 ccggatccgg aggtctgaga gggccgaaaa ggcgatctcg accgcctcct tctcggcctc
    19801 gatgatggcc cccgagagga ggctcccgag ccttctcgcc tccgccgcgg tgagctcggc
    19861 cgccgagggg gatcccttcc ccttctcgag gacatagagc tggaacttcc gcgagcccat
    19921 gaagatgatg gcgacccggt cccccttctc agtctcaagt tcgtattttg tcccgatccc
    19981 gggaagctcg atgcacctga cacccatatg tgcaatggtt ggagaaaaag ggataaaatt
    20041 cttctctctc gccgaaaagc cgcaaccggg ctttccgggg ccccggaaca gcgggcccac
    20101 cccggctccg gggggcgggg aagacggggg agagagaagt attattggga acttataaaa
    20161 aataataaat gagcaaacag ggcccatccc ccgtgcccgg tgcccggaaa atacatggat
    20221 ctccgcagga agcttctcat cgtcgtcggg gtcaccctca tctgtaccct catcgccttc
    20281 tatgccatca cccacggggc cgtcctcggg gccttcgtca ccctcgagga gaggaatacc
    20341 cagcagaaca tgaaccggtt catgaacctc ctctccgacg atctctcctc ccttgactcc
    20401 gtggtgaggg cctgggcgcc gagggacgac acctacacct tccttcggga cgggaacacc
    20461 cagtacatcg agaagaactt cagggccgag accttcacga gcaaccggct caacttcatg
    20521 gtattcctga acacatcggg cgatatctcc tatgccaagg cctttgacct ctcctccggg
    20581 caggaagagt cccttccgcc gagcctcgcc gggatggtcg cgggaaacga gatcctccgg
    20641 tccttctccc agaacgatgc gagcctctcg ggtttcctca tgctcgccga ggggccggtg
    20701 atggtagcct ccgctcccat caccacgaag aactacgagg gggtgagccg gggaaccctg
    20761 gtggtgggga ggttcctcga cgataatgag atccggcgga tcgcccagct cacgaaccag
    20821 caggtcatga tgtacaggcg gagcgacccc ggaagccccg ctgacgtgcg ggaggcccag
    20881 ctggagctcg acgggggccg gaccatctgg atccagccgg acagcccggg aaccatcgcc
    20941 ggctatgccc ccgtggagga cctctaccac acccccatcc tcatcatcaa gacccagatc
    21001 ccccggcaga tctatgccca gggccaggca accctggatt acttcaccct ctcgtacatc
    21061 atcctcatcc tcatctttgc catcaccttc ctgtacctcc tcgaccgctc catcctctcc
    21121 cccatctccc tgctctccac cgacgtgaag aggatcggga tgacccggga cctgtcggcc
    21181 cggctccggg tcgggaagga ggagaacgag atcagtaacc tccaggagaa catcaacaag
    21241 accctcgccg ccctcgagaa ggcccaggga cgcctccaga agagcgagga gaagaaccgg
    21301 gccctcctcg ccgcggtccc cgacctcatg tgtcaggtga ggcgggaggg gatccatgag
    21361 gagtggaact cgggaacggc cgccgagctc ggggtgatgg ccctccacga gctcctgggg
    21421 aacgagctcg aggagataga ggagaccttc gagctgatac cccaggaggg acagggcctg
    21481 ccggatgccg gagccgggaa cggggaggtg tcgcccgtct tcatcttcga gtacaagacg
    21541 aaggggaagg gtggcatgcg gtaccacgag atccggctcg tggtatccgg gaaggacgag
    21601 gtcctcgcca tcgggcggga catcaccgag aggaagcagg ctgaggagga gctggagcgg
    21661 taccgggccc ggctcgagga gctcgtggga gcgcggaccc aggcgctcca gcgcacgaac
    21721 gagcagctca ggcaggaaat cttccagaga aagctcatcg agggccagaa gagggaggcc
    21781 tacgagcaga tcgagaagaa catcgagcag ttcgccatcc tcggggacca catccggaat
    21841 cccctcgcgg tcatcgtggg cctcgcggac ctgaccgagg gggcggaggc ggcgaagatc
    21901 atggagcagg ccagggtcat cgacgggatc atcaaccagc tcgaccgggg ctggatcgag
    21961 tccgagaagg tccgggagtt cctgagaagg tactattcga acgataagga ctcggcgaaa
    22021 taagagaata tttttggacc ggatcgattt ttagagaagc atcaaggggt tcaaaattcc
    22081 ggaccctttt ttaggttggc atcagggggc tcatccgccc cccgccgtac ggtcacacca
    22141 tccgtaggct ggaatatgcg agtgcggttt gcgggaagat ctgagatccg atgacagggc
    22201 tatcctctct gggggacgcc gtagcggcgg gggtgagcgg agcgaacccc caggagcgtc
    22261 caggtatcac tggacccttt taacattaaa attaaggata aaccccggat cgcctatacg
    22321 atcagcctct tcttcatcag gttcacggcg aggatgaagg ccacggccgc gaccccgacg
    22381 atccacgcga gggagaggag gatctcgggg gtgacctgcg agagggtgac cattctcgtg
    22441 atcttcacca cgtgggtaag ggggaggaag gcggtcgcaa agacctggat gggagcagga
    22501 aggatggtca gggggaagaa ggttccggag aagaggaaca tgggggtgac gaagaggaaa
    22561 gtggggaggt tcagggtatc gatgttcggg gtgagggccg tgaagcacat gccgatggag
    22621 gcgaagagaa acccggcgag gaaggcgaag gggaggatgg agagggagat ggggaggtcg
    22681 accaccccga agaggacgag gaccgggagc atgatggtcg cgttgatgac gctccgggtc
    22741 gccccccaga ggatctcccc ggtgatcacc tcctcgatgg agaggggggt cgcgatgatg
    22801 gcgtcgaagg tcttctggaa gtacatccgg acataggagg cgtaggtggt ctcgaagaag
    22861 ccggcgttca tgatgctgat ggcgaggagg gccggggcga tgaaccgggg ataggagacc
    22921 ccgttgatct gggagacgaa gatcccgagg ccgtacccga gggagaggag gtacaggagc
    22981 ggttcgagga ggggcggcag gaagttcacc cggaaggttt tcacgaatac cttccggttc
    23041 cgctgccaga cccggagggt tcttccggtc aggtgtggtg gggtgaggta gccgagccgg
    23101 ctcattcctt gagcctccgc cccgtgagct tcaggaagac gtcctcgagc ccggcgggtc
    23161 gcgccaccac ctcgatctgg gtacactcct gcatgagggt cgccgtgacc tccctcggcg
    23221 tttcggtgaa cacctggacc cgctccccgg ccacctcgta cttcgccccc atcttcccga
    23281 ggcaggcgag gacctcgggc cggttgtccg cctccacgac gtcgatcccg gcatgcttcc
    23341 ggacgaggtc ggccggccgg ccgatctcga ggatcttccc gaggtccatg atcacgatcc
    23401 ggtcgcagag ctgggcggct tcctccatgt agtgcgtggt gaggacaatg gtgttccccc
    23461 gggaacggag ggcccggagc cggtcccaga tgaggtgccg ggcctgggga tcgaggccca
    23521 ccgtcggctc gtccaggatg aggagccggg gattgttgat gagggcgcgc gcgaggatga
    23581 gccgccgctt catcccgccc gagagcttct cgatggaggt gtcccgacgc tccgagagct
    23641 ggacgaactc caggaggccg tcgatggcct cccgggcggt ggagggggga atatcgaagt
    23701 agcgggagta gaccatgagg ttctcgtata ccgagaagtc ggggtccagg ttcgtctcct
    23761 ggggtacgac cccgatgagg cccttgatct cggcagggtg ggccgacacg tccatgccga
    23821 agacctcgag cctccccgaa tccttcggcg agacgcactg gatcatccgc atcgttgtcg
    23881 tcttaccggc gccgttcgga cccaggaacc cgaagacctc cccctccctc accgtgaagg
    23941 tcacgcggtc taccgcgaca aagtccccga accgcttctc gaggttctcc gcgaggatga
    24001 cgtccgccac gccggtcacc aagcccgatc ctttcccggg tcatcgcgcg ccctgataaa
    24061 ccctccccgc ccgggagggg aggaacgggg tcaggaggtc cgcacggcgg ccccggtatt
    24121 cctcggggcc gagatccagg catctccgcc gccgtgctcc ccgaggaact tctccgccgc
    24181 ccggaggatg gcggggccgt ccgagtcggt gacggcgtag accgtcgggc cgaaggagga
    24241 catcccggcg caggcggccc cggcggccct catttcctcc atcagccggg ggatcaccgc
    24301 cggctggagc ccgatctcga cccgtttgaa gccgagttcc tggatccggt tcaccgacct
    24361 cccgaagagg tccaggtccc ggtccacgac ccccgggagc atgcgcatga ggatctcgtg
    24421 gcaggtctcc ctcacctcct ccaggggaac ggggcaggcc tcgcggaaga tggcgagctc
    24481 cttcccgccg ctcgcccccg ccgggaggca ggggatggcg aggacgatcc gccagtcggg
    24541 ggggaaatcg tgcctcgcga tgaccggggc gggacgcacc ccgccggatg cgcccgaggg
    24601 ccggaagtcg ctcttctccg cgccgggtcc gaaggcatgt cccccatcga taatgaagcc
    24661 tcccccctcg aaggccgcgg tcccgatacc ggaggtcccg cccctgccta ccagccgggc
    24721 gagctcccct acagggaggt tccggccgaa cagctcgcag attccccggg ccacggcgag
    24781 ggcaatctgg gacccgctcc caagcccgac gtgggagggg tagatcctcc ggagggtgat
    24841 ctcgatccct ccggccgccc ggatcccctc gaggacccgg gccgcggcct tttccgccgt
    24901 ctcccgggaa cgccgatcac ccccccgcgc gacggtctcc ccggactgcc tcacgtcgag
    24961 aaggatcccg ggttcctcga gggcgatccc gatgcccccg tcgaccctcc cgatcccgcc
    25021 gtgcatgtcg atgagcccca ggtggatccg ggagggggtc tccacgatca cccgccgctc
    25081 atcgaggaag gcgtggtacg ggaactgctc ctcgatggaa atgaggggct ccccccggtg
    25141 gatgatctgg tagcgccggg agaggagcgg ctcgttcctg aagacgccga agaccttcat
    25201 gtgctcgggg gaggcgggga cgatcctcgc gtccaggatc tcccggcggg cctcgatgtg
    25261 gtgggtggcg aggatcctcc cgatggggat gtccgcctgc atgagatcct ccctgaactc
    25321 gggggagagc cggccgaggg gagtgtccga gacggcgtag atcaggggaa gggaggtctc
    25381 ggcgtcctcg aggctcacca cccggtggtt caccggctcc ccttcggcga cccggagaag
    25441 ggccgcggac tcgttcccgg cggggatcac ctcctgggag agggtggtga tcttcacacg
    25501 gccgcctgtg atcacctcca ggatccgggt gacggagccg tcggtcccga ggaggatctt
    25561 ctgcactctc gagagccttc cgatctcccg ctccagctcc cggatccggt cgtggatttc
    25621 catcgagctt catctcatgg cttcctttat ataaaccgat ccaaaaggga tccatccggg
    25681 agtcgccagg ccgtatccgc gacggctccc gtgagaaacc aggggatcgt caggggatga
    25741 tctcgcagcc gttgtaccgg ggatgggaga aatccgccat ctccagggtc ccttcccgga
    25801 tgagcctcct cacccggggg aggacaccgg tgaggccccg gtgaaggttc tccaccgttc
    25861 cgcagaccac ccgctgcagg tcctcgggcc aggggcggag gatccgggca tagaggcacc
    25921 tccccccgca gaagccccgg atggcgcagt ccgtgcagaa ggatccggtg tccgacggca
    25981 tggacagggc cagggggtcc gaccacccga tatgcccgag gtagaaatcc ttcatcccgg
    26041 ccatgcaggg acaggggccg atggacccgt cggtcatgat ggtgaggttc gcatgccccg
    26101 ctccgcagcg gagcgggctt tcccttccgg tgaggaggtc ctccatgggg tcaatgaagg
    26161 ggtaccacct catgacctcc ccggcggcct ccatgtgtcc gacccagtcg tcgatcaggg
    26221 ccagcacgcc cggattgtag accccttccg cccagtcggc aaatcccacg ctgccgttcg
    26281 ccggggagag gtccgcatcg agctgccagt ggatggacgg gaaggagaag tcccggttcc
    26341 cggagaggaa ccgcaccgac gaggcgatat cggtcccctc ggccaccgtc atcctcgcta
    26401 tgatctcgcc cgggtatcct cccgccacca ccctcctcag gttttccatg atccggcgat
    26461 aggtcccctc cccccgggat ccatcggtga ccgcctgggg gccgtcaacg gagaccagga
    26521 gggaatcgat ccggcccatc accgaacggg ggatccggtc gaggaggagc ccgtttgtct
    26581 ggacaaggaa ccgtttcgcc ggggcatgct ccaccatctc ggagacgaga tccgaccgga
    26641 ggaggggctc cccgccgtaa aagatgaggg aggcatccgg atccttctcg aggaacccgt
    26701 agagaagccc gggctcgtag ccgagctcga caggaagatc gaggtcgatt ccgggcgcct
    26761 cctcccccgg tcccgagggc tggaaggccc gtccgcggca gtaggtgcag gaaaggttgc
    26821 attcatccgt gaggattacg tggtagtaca cgggattccg caggatattt ggtacccggg
    26881 gaaaaaaagg tgcccgggcg caacggtcat aagggaaaag ggatattcct tccgcatatg
    26941 atcaccctct ccgatatccg gcgggccaga gagaggatcg ccccccacat catcaggaca
    27001 cctctcgtcc cgtcccccac tctctcctcc ctgtgcgggg cccgggtgta cctgaagctc
    27061 gagacgatgc agaaggccgg atccttcaag atccgtgggg ccatgaactg tatcctctcc
    27121 tgcacgagtg ggatcggcgg aaagggggtc atcgccgcct cggccgggaa ccatgcccag
    27181 ggagttgccg tggccgcgaa ggccgccggg gtacaggcga ccgtggtcat gccggtctgg
    27241 gcatcggtct cgaagcaggc ggcgacgcgt tcctacgggg cacgggtgat cctctcgggt
    27301 gcgagcctgg aagagagcat ccggtatgcc cgcgaactcg cgggttcggg gatgacctac
    27361 atcccggcct tcgatgatcc cgagatcatc gccggccagg gtaccatcgg cctcgagatc
    27421 ctcgatgacc tcccggagac cgacctgatc gcggtaccgg tcgggggagg ggggctcatc
    27481 gccggtatcg cggtcgcggc gaaggggatc aggcccgggg tccgggtcct gggggtccag
    27541 gcggcggcct gcccgtccgc cgtggaggcg gtgagggccg ggaggccggt gctcgtggat
    27601 gcccgcccga ccatcgccga cgggatccgg gtcaggcagg tgggggacct cacctttccc
    27661 tttatccggg acctggtgga ggagatcgtt gcggtcgagg aggaggagat cgcgaaagcc
    27721 atgctccttc tcctggagag gaaacgggtg gtcgcggagg gtgccggggc ggtgaccctc
    27781 gccgccctcg cctccggaag gctgaaagtc cgggagggat cccgggtcgt cctcgtcgta
    27841 agcgggggaa acgtggacac cccgctcctg gaccgggtga tccaccaggg cctcatccgg
    27901 aacgggagga tcctccaggc cgaggtggtt ctcgaggatg cgccgggggc tctcgccggg
    27961 ctcctggggg tggtggccgg gataggcgga aatatcctcc agatcaccca tctccggggg
    28021 ggccgggatc ttccggtcca ggcggtccgg gtcgctctcg acatcgaaac gagggacgag
    28081 gaacacggga gggagctcgt gaaggggatg gagggggccg gataccgggt ggagagattg
    28141 tagcgatccg gtcacgaggg tggaaagcga gacagtcggt ctccggggtt attcctccgt
    28201 tttgagccac cctatgggat gaaatgaaac gatgaccact aatcacaccc ggatcacata
    28261 tcggggtcca gtccccccac gggaagcctc ccagccagcc ccaataacca gttcgtttcc
    28321 ccaattgcag gcagaaaggt ccgtaaagta tttaaacctt cgaaacgcta aatcagttat
    28381 acaaacctaa aatcacattt gaggagtaaa aatgggacag aaagttggat ctgaaaagat
    28441 taagcgcgag aagggctacc tgtactacct ggggaaggac ggctacgtct gggccgctcc
    28501 catgaagaac aaccccggcg gcaggaagaa gaaggtcggg accgagcgta tccccaagga
    28561 catggggtac ctctactaca tcgacaaggc aggcttcgtg gcaaaggcca gaatgaagaa
    28621 cgcctgaatc tcaccatttt tcttcggttt ctcccggacc ggattcaccc gtgacggtaa
    28681 gggccgtttc cccgtgggtc atcgccatcc tgaagggagg tttttcaggc caggacgaac
    28741 tggaccgcag tcgggtattt ccgggctatg gcgtacgcga tggcccgggc gatccagagc
    28801 cgtccatcgg tgagcctccg gccctcgcca agatcgccat ggatctttag tgcccggaca
    28861 gcggtctcat cggtgaggta gcagctgtcc cggacgtcca cgtccacgaa aaagagaagg
    28921 ggtatcttca cgctcttccg gatctcctca gggagcccgg cggcgagggc cgagaggaca
    28981 tcgcggtcaa aggggtgccg ggctcctccc ttcgtcaatg ccgcaggctt ctcttcagcg
    29041 aggagggagg cgagggtttt acgctcggcg acaacgctgt cgttgatccg tgcggcctcc
    29101 atgcccatcc agcgggtgag cacggactcc tcggtgatcc gggggcgtct ggtcatcggc
    29161 ccgcaggctg gaactcccgg agtttctcgg ccatctcgcg gaggcgcttg tccaccaggt
    29221 agttcaccgt cccctcctcg aaggtcccgt cctccctcct ctcgccggcc gggacaccgg
    29281 tgaggacctg gattccctca tcgatggtct tcacggggta gatcctgaat ttccctgcct
    29341 ttgccgcctc caccatctcc tccttgagca tcaggttctg cacgttgctc gcgggtatca
    29401 tggcgccctg ctctccggtc aggcccttcg ccttgcagac ctcgtagaat ccctcgagct
    29461 tctcgttcac gccgccgatg gcctggacct cccccttctg gtttaccgag cccgtcacgg
    29521 cgagggtctg cctgatggga aggccggaga gggccgagag gatggcgtag agctccgtcg
    29581 aggaggcgct gtcgccgtcg acccccccgt agctctgctc gaagacgagc cgtgcagaga
    29641 ggctgaaggg cttgtcctgg gcgaatttgc cggcaaggta tcccccgagg atgagcaccc
    29701 ccttcgtgtg ggtgggccct cccatggccg actcccgctc gatgtcgagg accccgtccc
    29761 tgcccacgcc gatgctcgcg gtgacccggg agggggtgcc gaaggcgaag tcaccgaggc
    29821 ccataaccga gaggccgttc acctgcccga cctttgcgcc tgtcgtgtcg atgaggaaga
    29881 tgtcccgctc gatgaactcc tggatcttct gctggatgag gtttgagcgg taggtcttct
    29941 cgtcgatggc cttcatgacg tgctctgccg tcgtatactc cgcattctcc cgggtggcat
    30001 agaagctcgc ctcccggatg atgtccgaga cgctcgcgaa gagggtcgag agcttcttct
    30061 ggtcctcggc gaggcgcgag ccgtactcga tgatctttgc gacggccgtc ccgtccaggt
    30121 gcttcaggtt gaacttcctg acgagggtgc agatgaaggc ggcgtacttc atggagttct
    30181 cgtcgttccg gtccataacg acgtcgaagt ctgccctgac cttgaacagg tccttgaaat
    30241 ccggatccag ggtgtagagg agctggttga tctgcggggt cccgatgagc acgagcttca
    30301 cggagagggg gatcggctgg ggcttcaggc cccgggtggt gatgaagccc atcctctccc
    30361 ccggctcctc gatggcgacc tcaccggtct tgagggcggt cttgatcccg tcccaggaaa
    30421 aggggttcct cagcaggtcc tcgaccggga tgaccatgta cccgccgttc gccttatgga
    30481 ccgatccggg ccggatcatg gtgaagtcgg tggacacgat cccgaactgg acctcctttt
    30541 cgagcttccc gaagaggttc tggtaggtgg ggttctgctc gaagatgacg ggtgccccgt
    30601 ccggtttcga gttatcaacg atggcattca cctcgtattt cttgaagggg agctccttga
    30661 agaaggcctg gagctggggc gggacctgcg gtggctgctg gggcgccccc aggaactggt
    30721 cgaggttatc gaggatatcg ttctggacgg catcgatgta cttcaggatc tcggggaggg
    30781 aattgtactt ctctttgagg tcggtgatga ggtggccgat ggcatagagg gagacctccc
    30841 ggttcagctt gtcaacggcc tcggccccct ttgagtcgag atccctgacc tgccggaagg
    30901 cattcctgag atccgagttg agcttttcca tcctccgctg gatctccgcc ttcgtttcct
    30961 cggggagggc gacgaactcc tcctcgggga ggggtttccc gtccacgacg gggatggtga
    31021 ggagcccggc ggggctcatc tggatgacga agccctgctc ctgggcgctc gcctggatct
    31081 gcgcaaagag ctttgccttc tcctcgtcga tggatctgac cgttgcctcc ctccgggtgg
    31141 catagtcctc gctctcgaag gccttgggaa ggttcttctt cacctcgttg atgaagtccg
    31201 acatctccgc cttgaactcc gagcccttcc cgggggggag cagtatcgcg gtaggctcgt
    31261 actggctcgt gaaactgttc acatagaccc agtcgccggc cttgggcttg gtcttcgcca
    31321 gccgctcaag gaagtccatg acggccgtct ttcttcccgt gcctgcgatg cccgcgacat
    31381 agatgttgaa accggcctcc ctgatctcaa ggccgaattt aagtgcccgg agtgccctct
    31441 cctgtccgat gatctcctcg aggggctgga gggtgtccgt cgcggggcat tccttccggc
    31501 cggagtcata ggcgtttcgg aacctgtcgg ctggcagaac ctcgaccata gatatcaacc
    31561 atccaatggg gaatgaaata accgatctct ataagaatcc atcggcattt cgcaaattac
    31621 gtgaggtggg atgcctcaat tcccgggaac agggggtagg ttaatatgga agattcgaga
    31681 atctcaggct cggaaagacc ccccgatgtt ccggcgggat ctttaaaaac ggtggtacta
    31741 ggtggcacgg agatttgagg cctgtaaaaa gtcgggaaac ctcatgaagg tcaaaaaaaa
    31801 tcccgatatg atcagaacgg aactgaggga agcggagaat gacctcgaga tcgcgggaaa
    31861 gtccttcaag ggtgacaatg acaaatgggc ggtgaagcag tcccaccggt ccctcctgca
    31921 cgtgttcagg gccctccttc tcatgaaggg gtacgaggcg aagggtgtgg actgtctgaa
    31981 gtacgccatg gacgagctct acgtgagaaa gggggttttt tctgccgaca tcctcgagga
    32041 cttcgagtac ggccgggaga tcgcgaaggg gaatgactgc ctctgcgtct atgaccggaa
    32101 cgagatcgag gagatcctcg ccgtgaaccg ggtcctcctc gagcaggcga cggagatggc
    32161 gaagaaggcc tgaaaaccgg aatattcagt cgcgttcggc tccggatcag ggcggtgagg
    32221 cggggcagcc ccaggggcca taccggccga ggccccattc ccaggtgttc gtatcccggc
    32281 agaccgcggg tttcgtcggg tggatggaac agagataccg ggagtcatcc gccttcacga
    32341 ggaaggggca ggcctcgaga gaagtcccgg tggttggatc ggtccagccg gtggcgggtt
    32401 ccatgccccc cagctctgca tcggacagat ccgcagctgt cttcatggtc ccatcaggat
    32461 tcctgcctgc ggaaacgtat tgcatgatgt cccgcctgcc ttccccgtgc caccggagga
    32521 ggtcggggac cttcgcccag tgaaaacccc ggtactgcag gcagcaccac ccgcactgga
    32581 tacacggcat cgcgcccggt acctcagcat ccgcagggaa atgcccttcg gatggtaggc
    32641 ttgggttccc ggatagtgcg aatccggggt gggcggtgaa aagggggctg tttccggccg
    32701 ctgaccggga tccccgatga tatgtacgac ggccg
//
LOCUS       DATGDI010000042         6695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_178581, whole genome shotgun sequence.
ACCESSION   DATGDI010000042 DATGDI010000000
VERSION     DATGDI010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178581"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VKO45_01565"
     CDS             complement(<1..293)
                     /locus_tag="VKO45_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HMA04594.1"
                     /translation="MEITFVRVADNAARFYLAGAWPALANSLRREMISGVPTLAIDDI
                     RIYDNTSVLFDEMLAHRLGLIPLRTELSTHVPKDQCSCQGAGCPACTATYTLSV"
     gene            complement(296..691)
                     /locus_tag="VKO45_01570"
     CDS             complement(296..691)
                     /locus_tag="VKO45_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HMA04595.1"
                     /translation="MAGEKEKWGIAHIFASFNNTIITITDLTGAETVTKSSGGMVVKQ
                     DRNESSPYAAMQMAANVASAAMEKGIVGVHVKVRAPGRGKQRSPGPGAQAAIRALARA
                     GMRIGRIEDVTPVPHDSCRPKGGRRGRRV"
     gene            complement(694..1239)
                     /locus_tag="VKO45_01575"
     CDS             complement(694..1239)
                     /locus_tag="VKO45_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HMA04596.1"
                     /translation="MGYPGKNRKQYEKPTRKFEKQRQADEVKLLITYGLRNKREVWKS
                     QMVLRRFRRAARDITALSSAGVDPKIVEAKKRQMFDHLERMGFIGADASIEAILSLKV
                     EQQLERRLQTIVYRKGLARSPKQARQFITHGHIMIGTKKVTIPGYHVSRAEEPQISYA
                     THSPIANEVHPERARISKVGR"
     gene            complement(1253..1696)
                     /locus_tag="VKO45_01580"
                     /pseudo
     CDS             complement(1253..1696)
                     /locus_tag="VKO45_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
     gene            complement(1858..1942)
                     /locus_tag="VKO45_01585"
     tRNA            complement(1858..1942)
                     /locus_tag="VKO45_01585"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1906..1908),aa:Ser,seq:gct)
     gene            complement(2382..3620)
                     /locus_tag="VKO45_01590"
     CDS             complement(2382..3620)
                     /locus_tag="VKO45_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HMA04597.1"
                     /translation="MKLFGLDPEKGRWVFVVLGLLINLCLGSIYAWSVFRLPLQTLFS
                     VNATESLVPFIIFLALFAFVMPVAGGPLDRYGPRRLTILGGIVVGIGWILGSLSSSMT
                     MLAVTYGIIGGAGVGIAYNAPISVAARWFPDRRGLAVGATVLGFGISPIVTAPLANYL
                     ISLSGALPTMMYLGIAFLILIVLLGLPLRFPPLDWKPTGWTPAAGAGGPACDLNRGEM
                     VKTRCFWGLWICYVIGTLAGLMAIGIASPVGQEVFALTPAVAASFVGLFAFFNGIGRP
                     VFGHLVDRITPRFTAILSFALIAAAALLLGGGAGGGIAVYLVGFSALWLCLGGWLAIA
                     PACTAAFCGTKYYGPNYGLVFTAYGIGAIIGNLLAGRMRDVMGSYVAVFVPVAILAVI
                     GAILAFFLMHLPAASPEKAG"
     gene            complement(3760..4536)
                     /locus_tag="VKO45_01595"
     CDS             complement(3760..4536)
                     /locus_tag="VKO45_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA04598.1"
                     /translation="MTGRKNVKDVMARAASIAKSATITEALGKMLDEGIDPLIVTNNG
                     EVIGTISKRSIAEILGTRRNAQISPTKIHVANHVQGNFTSVYPDQSVEILVPLLQASR
                     VVVVLDENHRLIGQVGYADLLRVMKPGIHLERLLEKAYTIQADERVVHLRRRMLDDGI
                     TRFVVTSDGSIAGIVTETDVATALANFREVVETKYQDARIRNLIVRDIMSAPVIAAEK
                     TVPVADAVDLMLGKGISALPITETGQLAGLLTLESLVKAL"
     gene            complement(4533..5375)
                     /locus_tag="VKO45_01600"
     CDS             complement(4533..5375)
                     /locus_tag="VKO45_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA04599.1"
                     /translation="MIASDIMSAPVYVVGPAENVAHARRLMLSHRISRLPVLDGDRLV
                     GILTKKDLAYRLRQTDPVWRRRPIDKIPVHVLMVPDPVSVTPEARVPDIAALMVARDF
                     SGLPVLDGKKLVGMVTKTDILRSASVNSITLKVGELMGDAVTVNRYHSLDHVINLMSE
                     RHDPLVVVNDDGTLAGIITESNLAFYKYNTDTSTGLPEKDVKLLRKGESGGRKRLRYV
                     MEVSAVAEDIMTRPVITIGREDPVTAAVDLMRKHQIHSVVVVDGANLAGVLERDNIIR
                     EVAK"
     gene            complement(5378..6322)
                     /locus_tag="VKO45_01605"
     CDS             complement(5378..6322)
                     /locus_tag="VKO45_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA04600.1"
                     /translation="MNRNNRINERQGDRQLRLPGNSDRGPVNFESRKAPREGEIMAIA
                     SRDVVSVPPTIPIIGAIEKMTACGFRRLPVTDAGTGRLRGIVTAGDIIDMMGGGNKFN
                     LVRKKHAGNLLAAINESVREIMTEKVVTIGKDARIADAASLIVEKRMGGLPILDAGGV
                     LAGIVTERDVMKVLSSERSATLVEEAMSGSLKVTGPETPIGEATKQMITHRFRRLPVV
                     SEDVLYGIITATDIMKYLGTGQVFQRMVTGNVAEVMAIPVRTLVSGTLHTIDPKKNIN
                     EAAREMLARNVGALPVIEDARLVGLITEFDLVKAFLRR"
     gene            complement(6344..>6695)
                     /locus_tag="VKO45_01610"
     CDS             complement(6344..>6695)
                     /locus_tag="VKO45_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA04601.1"
                     /translation="VTIDSDEVIQKVYTLMMESGFTAFPVMKKKQLVGIISRHDLLAA
                     GRVRKALDNISRVKVESVMTKEVITIAPAAPTAEAAGLLVRHDISRMPVMDGGRLAGI
                     IDRHDVLKGLVFKQ"
BASE COUNT         1123 a         2146 c         2147 g         1279 t
ORIGIN      
        1 accgagaggg tgtaggtggc cgtacaggcg ggacagcccg caccctggca ggagcactgg
       61 tccttcggga catgggtgga gagctcggtc cggaggggga tgaggcccag ccggtgggca
      121 agcatctcgt cgaagaggac gctcgtgttg tcgtagatcc ggatatcatc gatggcgagg
      181 gtcgggacgc ccgagatcat ctccctccgc agggagttcg cgagggccgg ccaggccccg
      241 gcgaggtaga accgggcggc gttgtccgcc acccggacaa aggtgatctc catgtctaga
      301 ctctccttcc ccgccggccc cccttcggcc ggcagctgtc gtggggcacg ggggtcacgt
      361 cctcgatcct cccgatccgc atgcctgccc gggcaagggc ccggatggcg gcctgggcac
      421 ccggcccggg gctgcgctgc ttcccgcggc ccggggcccg gaccttcacg tggaccccga
      481 cgatcccctt ctccatggcg gccgaggcga cgtttgccgc catctgcatg gccgcgtacg
      541 gggaggactc gttccggtcc tgcttcacca ccatcccgcc gctcgacttg gtcaccgtct
      601 cggcgccggt gaggtcggtg atggtgatga tggtgttgtt gaaggatgca aagatatggg
      661 cgatccccca tttctccttc tctccggcca tggttaccga cctaccttgg agatccgcgc
      721 ccgctcaggg tggacctcgt tcgcgatggg cgagtgggtg gcgtagctga tctggggttc
      781 ctcggcccgg cttacgtggt agccggggat ggtaaccttc ttcgtcccga tcatgatgtg
      841 gccgtgggtg atgaactgcc gcgcctgctt cggcgaacgg gcgaggccct tccggtacac
      901 gatggtctgg agccggcgct ccagctgctg ctccaccttg agggagagga tggcctcgat
      961 actggcatcc gcgccgatga accccatccg ctccaggtga tcgaacatct gccgcttctt
     1021 cgcttccacg atcttcgggt ccaccccggc cgaggagagg gcggtgatgt cccgtgcggc
     1081 acgcctgaac cggcggagga ccatctggga cttccagacc tcccgcttgt tccggagacc
     1141 gtaggtgatg aggagtttga cctcgtcggc ctggcgctgc ttctcgaact tgcgggtcgg
     1201 tttctcgtac tgcttgcggt tcttacccgg gtatcccata gcttcccctc ctttactcct
     1261 tcttcctctt cacgccgacg gtggtcccgg tgcggcccgt ggacttcgtc cgctgaccgc
     1321 ggaccttctg gccggtctcg tgccggatgc cccggtagca gcggatcttg cgcatccggt
     1381 tcacgtcatc ctccttcccg atgaggacat cggtggagag gaggtgcttc ccctcgccgg
     1441 tgtacgggtc ggccgggcgg ttgagcatcc accgggggac ctgggggccg tagctatcga
     1501 cgacctgccg gagccggtcg acgcccgcgt cgtcgagctt ccccatccgg tcgagggggt
     1561 tcacccgggc gagccgggag atggcccttg ccgtgatcct cccgatgccc gggatcttgg
     1621 tgagcgcgat ctggaccgaa cgggtcccgt ccaggtccgt gttccggatc cgcacgaagt
     1681 acttgatgtc cttctccggt gctttttccg cggccttcgc gggggccgtc tccttcgcct
     1741 tctcgggaga cttccccttc cccttctccg gagccttctc cgagaccttt tccggagccc
     1801 tgtcctttgc ttttccctgg tcttccatcc catcgcccca cgggagatcc tgacgagcgc
     1861 tgagggaggg atttgaaccc tcgagtccca cagggacaca ggtttagcaa acctgcgcca
     1921 tacctggctt ggctacctca acagagaatc tcgcatcttt cgatactcgc gatgcagagt
     1981 ctgcaccgct ccatgaacgg cgtaactaat tggagaggtg tataaataag ggttgtggtg
     2041 gtcccgccga accgggatct atttccttgg tgaactctcc atttggctcc cacgattgat
     2101 tttattgggg tgcttcaggg ggggctacgc cacccccgcc gcacgggcac cccccagtat
     2161 ccggttccgg tgatcatacg gtgcacggta tccacaggct atcctcacgt gggggacgcc
     2221 tcagcggcgg gggttcgcgg agcgaacccc caggagcgtc cgccggtcgt gaatcaattt
     2281 cgaatctgga gataggttga aaagcttaat ctctttctcc ttggtatgct ttcggcgtta
     2341 agtgagtcga gggcagggta atcgaaaaaa aggaaccggg atcacccggc cttctccggt
     2401 gatgcggcgg ggaggtgcat caggaagaag gcgagaatcg ccccgataac ggcgaggatg
     2461 gcgaccggga cgaagaccgc cacgtacgag cccatgacgt cccgcatccg cccggcaagg
     2521 aggttcccga tgatggcgcc gatgccatag gccgtgaaga cgaggccata gttcgggccg
     2581 tagtacttcg tcccgcagaa ggcggcggtg caggccgggg cgatggcgag ccaccccccc
     2641 aggcagagcc agagcgccga gaagcccacc aggtagacgg cgatcccgcc gcccgctccc
     2701 ccgccgagga ggagggcggc ggccgcgatg agggcaaagg agaggatcgc cgtgaaccgg
     2761 ggcgtaatcc ggtcgacgag atggccgaag accggccggc cgatgccgtt gaagaacgca
     2821 aagagcccga cgaaggaagc cgccacggcc ggggtgaggg caaagacctc ctgcccgacg
     2881 gggcttgcga tcccgatggc catgagcccc gcgagggtcc cgatgacata gcagatccag
     2941 agtccccaga agcaccgggt cttcaccatt tcgccccggt tgaggtcaca ggcgggacct
     3001 cccgctccgg ccgccggggt ccatcccgtg ggcttccagt caagcggcgg gaatctgagg
     3061 ggcagcccca ggaggacgat gaggatgagg aacgcgatcc ccaggtacat catggtcgga
     3121 agggcaccgg acaggctgat caggtagttg gcgaggggtg cggtgacgat gggcgagatc
     3181 ccgaacccga gcaccgtcgc cccaacggca agccccctcc ggtccgggaa ccagcgggcg
     3241 gccacgctga tgggggcgtt gtacgcgatc cccacccctg cgccgccgat gatcccgtag
     3301 gtcaccgcga gcatggtcat gctcgacgag aggcttccaa ggatccaccc gattcccacc
     3361 acgatacccc cgagaatggt gagcctcctc gggccgtacc ggtcgagggg cccgccggca
     3421 acgggcatca cgaaggcgaa gagggcgagg aagatgatga agggcaccag gctctcggtc
     3481 gcgttcacgg aaaagagcgt ctggaggggg agccggaaga cgctccatgc atagatgctc
     3541 ccgaggcaga ggttgatgag gagccccagg acgacgaaga cccaccttcc tttctcaggg
     3601 tcgaggccaa agagtttcat ggaaccaccg taccgggatc acgggaatcc cgatttaaca
     3661 tgattaataa tgaatattat gtttaagaag attgcgattt attgcagcat gcgataagga
     3721 agggacagcc ctggacggcc ggcgaaaaag aaaaaaaccc tagagcgcct tgacaagcga
     3781 ctcgagggtg aggaggccgg cgagctggcc ggtctcggtg atggggaggg ccgagatgcc
     3841 cttcccgagc atgaggtcca cggcatcggc gaccgggacg gtcttctcgg ccgcgatcac
     3901 gggggccgac atgatgtccc ggacgatgag gttccggatc ctcgcgtcct ggtacttcgt
     3961 ctccacgacc tcccggaagt tcgcgagggc ggtcgcgaca tcggtctcgg tgacgatccc
     4021 ggcgatggac ccgtccgagg tgaccacgaa ccgggtgatc ccgtcgtcga gcatcctcct
     4081 ccggaggtgg acgacccgct cgtccgcctg gatggtatac gccttctcga ggagccgctc
     4141 gaggtggatg cccggcttca tcacccggag aaggtccgcg tacccgacct gcccgatgag
     4201 acggtggttc tcgtcaagca ccaccacgac cctggaggcc tggagcaggg ggacgagaat
     4261 ttcgaccgac tggtccgggt acacgctcgt gaagtttccc tgcacatggt tcgccacatg
     4321 gatcttggtc ggggagatct gggcgttcct cctcgttccc aggatctcgg cgatgctcct
     4381 cttcgagatg gtcccgatga cctccccgtt gttcgtgacg atgagcgggt caataccctc
     4441 gtcgagcatc ttccccagag cctcggtgat ggtggccgac ttcgcgatgg aggccgcccg
     4501 ggccatcacg tctttgacgt tttttctccc cgtcattttg ccacctcccg gatgatgttg
     4561 tccctctcga ggactcctgc aaggttcgct ccgtcaacca ccaccaccga gtggatctgg
     4621 tgcttgcgca tgagatccac cgcggcggtg accgggtcct ccctcccgat ggtgatcacc
     4681 ggccgggtca tgatgtcctc ggcgacagcc gacacctcca tcacgtaccg cagccgcttc
     4741 ctcccgccgg attctccctt ccggaggagc ttcacatctt tctcgggcag gcccgtgctc
     4801 gtgtcggtgt tgtacttgta gaaggcgagg ttgctctcgg tgatgatccc ggcaagggtc
     4861 ccgtcgtcgt tcaccaccac gagaggatcg tgcctctcgc tcatgaggtt gatgacatgg
     4921 tcgagggagt ggtagcggtt cacggtcacg gcatctccca tgagctcgcc aaccttgagg
     4981 gtgatgctgt tcaccgatgc cgaccggagg atgtcggtct tcgtcaccat accgacgagc
     5041 ttcttcccgt cgagaacggg gaggcccgag aagtcccggg ccaccatgag ggccgcgata
     5101 tccgggaccc tcgcctcggg ggtgaccgag accggatcgg ggaccatgag cacgtggacc
     5161 gggatcttat cgatgggacg cctcctccag acggggtccg tctgccggag ccggtaggcc
     5221 aggtccttct tcgtgaggat ccccacgagc ctgtccccgt cgaggaccgg gagccgcgag
     5281 atccggtggg agagcatgag ccgccgggca tgggccacgt tctcggccgg gcccaccacg
     5341 tagacgggtg ctgacatgat gtctgatgcg atcattctta cctccggagg aaggccttca
     5401 cgaggtcgaa ctcggtgatg agcccgacaa gccgggcgtc ctcgatgacc gggagggccc
     5461 cgacgttccg ggcgagcatc tcccgggccg cctcgttgat gttcttcttc gggtcgatgg
     5521 tgtggagggt gcccgagacg agggtccgca cgggtatggc cattacctcg gcaacgtttc
     5581 ccgtgaccat gcgctggaag acctggccgg tcccgagata cttcataatg tcggtcgcgg
     5641 tgataatccc gtagaggacg tcctccgaga caacggggag cctccggaac cggtgcgtga
     5701 tcatctgctt tgtcgcctcg ccgatgggcg tctcgggccc ggtcaccttg agggagcccg
     5761 acatggcttc ctccacgagg gtcgcgctcc tctccgagga gaggaccttc atcacgtccc
     5821 tctcggtgac gatccccgcg aggacccccc cggcgtccag gatgggaagg ccccccatcc
     5881 tcttctcgac gatgagggag gccgcgtccg cgatccgggc gtccttcccg atggtcacga
     5941 ccttctcggt catgatctcc cgcacgctct cgttgatggc ggcgaggagg ttcccggcat
     6001 gcttcttcct gaccaggttg aacttattcc cgccccccat catgtcgatg atatccccgg
     6061 cggtcacgat cccccggagc cggccggtgc ctgcatccgt gaccgggagc ctccggaagc
     6121 cgcaggccgt catcttctcg atggccccga tgatggggat ggtggggggt accgagacca
     6181 catccctgct cgcgatggcc atgatctccc cctccctcgg tgccttccgg gactcgaagt
     6241 tgaccggccc ccgatcggaa ttgcccggta accgcagctg gcgatccccc tgtctctcgt
     6301 tgatcctgtt attccggttc atgattccct acctcttttg cggtcactgc ttaaaaacaa
     6361 gacccttcag cacatcgtgc cggtcgatga tccccgcgag ccttcccccg tccatgaccg
     6421 gcatccggct gatatcgtgc cggacgagga ggcccgctgc ctccgcggtg ggcgcggcgg
     6481 gagcgatggt gatgacctcc ttcgtcatca ccgactcgac cttcaccctc gagatgttgt
     6541 cgagggcctt cctcaccctc ccggccgcga ggaggtcgtg ccgggagatg atcccgacaa
     6601 gctgtttctt cttcatcacc ggaaaggcgg tgaagccgct ctccatcatc agggtgtaga
     6661 ccttctggat cacctcgtcg gagtcgatgg tcacc
//
LOCUS       DATGDI010000043        17138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_178659, whole genome shotgun sequence.
ACCESSION   DATGDI010000043 DATGDI010000000
VERSION     DATGDI010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 17138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17138
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178659"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..477)
                     /locus_tag="VKO45_01615"
     CDS             complement(280..477)
                     /locus_tag="VKO45_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04602.1"
                     /translation="MTGRAMARAMRARRAGSVLFWVVGGGVHVNGGVVDATDVFVGVA
                     MVVGTVVWTGCETVNWAEPIY"
     gene            554..638
                     /locus_tag="VKO45_01620"
     tRNA            554..638
                     /locus_tag="VKO45_01620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:588..590,aa:Ser,seq:tga)
     gene            666..890
                     /gene="hypC"
                     /locus_tag="VKO45_01625"
     CDS             666..890
                     /gene="hypC"
                     /locus_tag="VKO45_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HMA04603.1"
                     /translation="MCIAIPAEVIELKGNNVGVVDYGDLRQEVQLDLVDVQVGDFVLV
                     HVGFAIQKLSREEALETREIFRQVYATLEE"
     gene            890..1231
                     /locus_tag="VKO45_01630"
     CDS             890..1231
                     /locus_tag="VKO45_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HMA04604.1"
                     /translation="MHEYSIAYDIYTTARRAALENKANVVKRIHVDIGEMAVVNAEQV
                     EFLFHAISEDDPLFRGAALVCRPVVPRGRCACGYEGDEVFVCPGCGAMPELVQGREIV
                     VTSLEIDVDGA"
     gene            1228..2223
                     /gene="hypE"
                     /locus_tag="VKO45_01635"
     CDS             1228..2223
                     /gene="hypE"
                     /locus_tag="VKO45_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HMA04605.1"
                     /translation="MKVNLAHGAGGETMGELLRTLTNVKNRNAGGIGLESLDDGAVIP
                     LGDQNVVFTTDSHVVRPIFFPGGDIGRLAVCGTVNDLAVMGGRPIALSCGMILEEGFD
                     IIDLERIVRSMDEALTESGAHLVTGDTKVVERGSLDKIIINTAGIGIAEKIVRDNGFR
                     PGDRIIVTGTLGDHGLAILTHREGFDLGEQIRSDVSPVWGLVEIALAAGDVHAMKDPT
                     RGGFAHAINDMARKSRTGVVIEEEALPIRRSVRSASEMLGIDPLEVANEGKIVMAVAP
                     DDAGAVLSGLRTHPYGREAAIVGTVVEGDRVIMRTRIGGERFIEPPLGDPVPRVC"
     gene            2217..3470
                     /locus_tag="VKO45_01640"
     CDS             2217..3470
                     /locus_tag="VKO45_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HMA04606.1"
                     /translation="MLDLLLRNVHLPEGRTVDISIEKGIVVHAGAGGRADSTLECSEF
                     LVLPGGVDMHVHMRGGSRESRKEDWGTGSRSALAGGVTLAVDQPNTNPPLTTVEALQA
                     RIGEAGRDSLCHFAVNAGVVPGADLPGLHRAGAMAFGEIFLGASTGAKGVSGGVLGPL
                     LSGIHALGTTATLHAEEVHEADDTDLVSHDASRPQEGEIRAIGKVKSRTPKGMLLHFC
                     HLSTAGALAAAAPHTTEVTPHHLLLSREDFPKDDPRGKVNPPLRSATEREGLLAAWNR
                     VTVLASDHAPHTREEKAGEFSRAPAGVPGVETMIPLMIPRVLSGEMTPASLIEKTSGN
                     PCRILGIPVPGFSGGHRADFALYPREITTIRARDLHSRCGWTPFEGRQAVFPDIVILG
                     GEEVLREGDFTRGNPRWFPGRGYHP"
     gene            3683..3997
                     /locus_tag="VKO45_01645"
     CDS             3683..3997
                     /locus_tag="VKO45_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HMA04607.1"
                     /translation="MEPFADAVTEDQMGVILALEVTPASERSSFFTGYDPWRKSIRCT
                     VRSPPSKGKANREVIDSLAGALGVPAASVRILSGATQHRKRVKIEGISRADALGRLDR
                     FL"
     gene            4166..5386
                     /gene="dnaG"
                     /locus_tag="VKO45_01650"
     CDS             4166..5386
                     /gene="dnaG"
                     /locus_tag="VKO45_01650"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HMA04608.1"
                     /translation="MYSSDTTKYLIRINLHAEGVVEKSDVVGAIFGQTEGLLGEDLDL
                     RDLQRTGRVGRIDVQIASKKGETKGEILIASSLDRAETAILAASLETIDRVGPCVAHV
                     SVEKIEDIRVTKRKMIVDRAKELLIDRFDEGSIDSTVLLDDVRESLRIEKVSEIGPDH
                     VPAGPNVLESDAIIIVEGRADVITLLRYGIKNAVAVEGTKVPATIADLCQTKTATSFL
                     DGDRGGELILRELLQVADIDYAAFAPRGRSVEDLSRKEVTKALRNKVPVEFVREQLAE
                     KPSEVRPPKVPSPEATPGIREWEGTARKREAPAKGGTSLRDHVEEVSGHGLARFISPD
                     FSVLAEVRAGDLERSLSAIKGDVTGIVADQVVDQRLLDQVAQKGIEFILARDFKGIIK
                     RPLPLKLIRFAQVT"
     gene            5590..5874
                     /locus_tag="VKO45_01655"
     CDS             5590..5874
                     /locus_tag="VKO45_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HMA04609.1"
                     /translation="MHKEELISLHQMMVEIKDFFEKINPELKFSQYYALKIDPSQVHR
                     SKMEHKYAIFILGTELANAMRNVEFGASGRISARMRELAEKTLKEIENLK"
     gene            6336..7271
                     /locus_tag="VKO45_01660"
     CDS             6336..7271
                     /locus_tag="VKO45_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HMA04610.1"
                     /translation="MRYYYGSEESLDYAEVNRAVEAVFREHARGHVQMPPKIYVTLEH
                     GDFRTMPAYVPALDMAGVKIVNVHPGNRAAGLPTVMALTVILDVETGVPKAIINATRL
                     TDMRTGAAGAIAAKYLAPKREVVLGVIGSGRQAEAQVRAISRELVITEVRVWSREEKN
                     ALALAAMFGDLSASATTIQKACDADVVVTTTPSTKPIVMHEWIHDGTHINAIGADAPG
                     KEELDPLILKRAKVFVDDRPQAIHSGEINVPIAKGLFHAHDIAGTLGEVVVGVKGRKR
                     PDEVTVFDSTGLAIQDLAIANLAMGAGKWVDLPFP"
     gene            complement(7295..8767)
                     /locus_tag="VKO45_01665"
     CDS             complement(7295..8767)
                     /locus_tag="VKO45_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA04611.1"
                     /translation="MLLKTTRTLCPVCGRTIPAEIVEEEGKVFFERTCPADGPFRDLY
                     WSDAALYRRFDEFESLGTGLANPHREAPEDGCPRYCGVCSNHRSGTLLANIDLTNRCN
                     LNCDFCFVNAQACGYVYEPTFDQVAGMLALLRDEKPVPTPAVQFSGGEPTLREDLIEI
                     IRKAKELGFKQVQIATNGVKIARDQGYAKALKEAGLSTVYLHFDGVSRETDPQIGRHR
                     EAVKVLDSVHLGVVLVPTIIKGRNDHEVGDIIRFAAEHISVVRGVNFQPVAFTGAASD
                     DDVRNARITIPELLERIESQTSGVIRKEDFYPVPCVVPISDLVEAYTEKPQIRFTAHQ
                     HCGAATYVFVTENGMVPINRMVDVDKFFAAVKKMTENLRKGGQINKYLTLLEGVNALR
                     TSVKKGENAGSPKIWKVLAGALLFQNFDALRDFHWNSLFIGTMHFMDRYNYDLSRVQR
                     CCIHYATPEGRIIPFCTYNSGPVYRDEIWRKHSRPLRKED"
     gene            8968..9480
                     /locus_tag="VKO45_01670"
     CDS             8968..9480
                     /locus_tag="VKO45_01670"
                     /EC_number="2.7.7.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
                     synthase"
                     /protein_id="HMA04612.1"
                     /translation="MLPAYIPNPAAVILGGGRSIDGGAVWRDGRRLFGDGKTVRGFVL
                     GVLAGVAAGGAGLWVQGLAGGAPAWSFLPSHTPLTVCTFALGALLGDLAKSFVKRRLG
                     KGQGDPWPVADQYDLVAGAFLLTALLAPAWLLATITLPVLVWILVLTPVLHRVTNMAG
                     HRLGVKRVPW"
     gene            9474..9989
                     /gene="pyrE"
                     /locus_tag="VKO45_01675"
     CDS             9474..9989
                     /gene="pyrE"
                     /locus_tag="VKO45_01675"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HMA04613.1"
                     /translation="MVKPLAALLAAHGAVRHGDFLLASGARSRVYVDIKSALTEPGVL
                     SVLALEIAGRGSFEAVAGVAVGGIPIAVAVSLAAEKPYAIVRSGEKDHGTGGRIIGDV
                     AGRSTLLVEDVTTTGGSVLAAVRLLRAAGARIDTVVTIVDREEGAAGALAAEGVTLVS
                     LVTLAELLAGG"
     gene            10015..11307
                     /gene="purD"
                     /locus_tag="VKO45_01680"
     CDS             10015..11307
                     /gene="purD"
                     /locus_tag="VKO45_01680"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HMA04614.1"
                     /translation="MVMKLLLVGSGGREHAMAEAITRDGEATLAAFMARSNPGIARLA
                     RSIRIGKETDGKNVRDFAVEAGVDYAIIGPEAPLEAGVVDLLEEAGISCAGPTRAAAR
                     LETDKAFCRDMMAAHRIDGCPRYRVFHDPDGAAAFIEDHDGDLAVKPIGLTGGKGVRV
                     MGEHVDRAGAVAYARSLGGNVVLEERLVGEEFTLQAFVDGKSLVPAPLVQDHKRAFEG
                     DTGPNTGGMGSYSLPDHRLPFVSEEDYRRALRIMEDAVRALAREGFPYRGILYGQFMN
                     TRDGPRVVEFNARFGDPEAMNVLPIMTTGLADVVCRIIEGTLSASRVGFEPAATVCKY
                     VVPEGYPEAPLSAQRVTLGDSGRARLYYANVDETDGSLATRTSRTLAFVGIAGTLEEA
                     ERAAEAAASSVKGRVYHRRDIGTRELLARRSAHMEAIR"
     gene            11304..12212
                     /gene="argF"
                     /locus_tag="VKO45_01685"
     CDS             11304..12212
                     /gene="argF"
                     /locus_tag="VKO45_01685"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HMA04615.1"
                     /translation="MRRDFLSVLDMDPHDLTGLLDRAARMKKERGGEEERRILAGKCL
                     AMVFEKASTRTRVSFEVGMVELGGHAIFLNPKDLQLGRGEEIRDMARVLSRYVAGVMI
                     RAFRHETIEEFARHASIPVVNGLSDREHPCQILGDLLTLREHFGRTDDLSVAWIGDGN
                     NVCHSLILASALAGFELTVASPARYRPDRGIVESARAAGGRIRLVTDPREAARNADAL
                     YTDVWVSMGEEEEREQRLAAFRGYTVDENLLSLASDGAVVFHCLPAHRGEEITDAVLE
                     GPQSIVWDQAENRLHTEKALLAALLG"
     gene            complement(12209..12487)
                     /locus_tag="VKO45_01690"
     CDS             complement(12209..12487)
                     /locus_tag="VKO45_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04616.1"
                     /translation="MAEEELYDVIIPPGVPRTIIIDISRKFDVKLVERKMSLNFANMD
                     GTEREILAFRGKREVVEKVEKYLKEQVIQFIETGGGFPRREEKKEGAT"
     gene            complement(12504..14000)
                     /locus_tag="VKO45_01695"
     CDS             complement(12504..14000)
                     /locus_tag="VKO45_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 14 protein"
                     /protein_id="HMA04617.1"
                     /translation="MAARFFDRFFRPKPHIVESPRPPSIAHGAGMNIPEYRVKPYFIC
                     ASVEMGNTTTKCILTGTDLETGIAYVINKTVRMSRDIRPPKPGEEIFGETIDGTPITR
                     QSVTEAVRDIIIECHREVHLDINCDLDFVVRSTGVVAEMETPDQVGDFVIALANGCLL
                     AGVPPRKMCPPMAKENLSLKLQPFSFTDKVVFVGAVAGVLPPLGSTGVEMVANEMEGE
                     LAMAGIKEGAKWTPVDFRNPCITIDFGTTLDGRITSDVPKDAVNPFAKTIGNFCGLAG
                     AIPDALVRGSGLVDPRTGCALDVFGEASSTGMFRARESALVEDYVERCRPHIDIRMVP
                     PDRRRFGRVPVYADVAAKSGVALIGCDCGENGGDIPKLVEIGAEIYENHGLSTIHEVV
                     DRVCADMALRLVDVARGENLVPPNSSIGFTGRAAISGNKPYLIVDGIAERGIYDDPID
                     HLVFVDDGLGRGAALMGRCMCSLGKPRNPLGGVRGGPCIMKRRMKIGK"
     gene            14224..15219
                     /locus_tag="VKO45_01700"
     CDS             14224..15219
                     /locus_tag="VKO45_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HMA04618.1"
                     /translation="MPGEFTLGLVVVLILVLAAEFVNGWTDAPNAIATVVSTRVLSPR
                     KAIVMATLLNIAGAFSGTAVAATIGTGIVDASVISLTTVAAAMISLVSWSTFAARFGL
                     PTSESHALVAGLAGAGLAVAGPAVLLWEGWEKVLIGLAFSTFLGFLGGWGITIAINRA
                     FLHSQRRGMREIFGRLQLLSAAFMAFGHGSNDGQKFIGAFVLALVIGGILPGFQVPFW
                     VVLLCSVMMGIGTSTGGWRIIRTMGMRMVKLEPYQGFAAETGAATVIQAASVFGIPLS
                     TTHTINTSIMGVGTVKGFSAVRWGVVRQIVLAWILTFPICGALSWGIATLVVRVL"
     gene            complement(15230..17062)
                     /locus_tag="VKO45_01705"
     CDS             complement(15230..17062)
                     /locus_tag="VKO45_01705"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HMA04619.1"
                     /translation="MRLLLIHSDHIDYEAKKPTSMAEDAKNLRDSMDEALVAFCAVEA
                     ADEEDMGDVVEQAVTDIAKTASQLDCRRVMVYPYAHLASDLATAGAAVEALEAVRQGL
                     SDLGLEVKRAPFGWYKAFTLTCKGHPLSELSRTLVAGGEKEEAVSKKEVEHEWFILTP
                     DGKRKDVKDYMDESPFGCLVKKELGVPVGAAGEPPHVELMRSKELVDYEPRSDVGHLR
                     WMPKGKLLRDLIADYVLTQVLPYGAMPVETPVMYDLGDQAICEHAAKFGERQYRFRSG
                     NRSMMLRFAACFGMFSIMNGMHISPNTLPMKMYELSTYSFRHEQKGEVIGLKRLRAFT
                     MPDMHTLCRDMDEALRCFEEQLVMGQKSGTDLAVRYYSALRCTRDFYQEHEAWVKKVV
                     RLVGEPTLIEILSERVHYWVAKIDLAAIDAQGRPIENPTVQIDVESSTRFGIRYHLDG
                     QEIHPPILHCSPTGSVERVICAMLETLAGEEVPQFPTWLSATQVRVIPVADRHKDVAL
                     ALCTRLNDQGIRADVDDREESMNKKVRAAGMEWVPYIVVMGDQEITSGDLTVTIRKLS
                     TPEKLHRERMKEDGLVTKVKDETAGKPFRPLYTPRLLSIRPRFI"
BASE COUNT         2936 a         5506 c         5576 g         3120 t
ORIGIN      
        1 catctacgtc accctcatca accggcagaa tgaaatcacc attacatccc cgttttccca
       61 gaccttcacc ggactcctgt ccattcgggg atccttgcgt aatcccagcc tttcacgcgc
      121 tgtggggaac aatgggaccc agctcagggt ggaccacaac ggtcaaaccg tcttcgggtt
      181 gcaatatatc gccacgctaa acggtgcctt ctccacggat attcctatca cagaaggagg
      241 gacctatact gcgtatttct acgactcgct gattcagggt cagtatatag gttcggccca
      301 gttcaccgtc tcgcagccgg tccataccac ggtccccacc accatcgcca ccccgacaaa
      361 aacatccgtg gcgtccacca cacctccgtt gacatggaca cctcccccga ccacccagaa
      421 gagcacgctc cccgcccttc tggccctcat cgccctcgcc atggccctcc cggtcatcgc
      481 caggcggagg taatcccaat tttttggagc cttgaaaggc ttttttaacc gctggcgcaa
      541 agtttctgag ggagccgggg tagtctagcc cggaaaggcg gcggccttga aagccgctgg
      601 tgcttggcac ctcgggagtt caaatctcct ccccggcgct tccattccgg aacggtgaca
      661 gagagatgtg catcgcgatc ccggccgagg tcatcgagct gaaggggaac aacgtcggag
      721 ttgtggacta cggtgacctc aggcaggaag tccagctcga cctcgtggac gtgcaggtcg
      781 gggacttcgt cctcgtccac gtcgggtttg ccatccagaa actctcccgg gaggaggccc
      841 tcgagacccg ggagatcttc cggcaggtct acgcgacgct ggaggagtga tgcacgagta
      901 cagcatcgcc tatgacatct acaccaccgc ccggcgtgcc gccctcgaga ataaggcgaa
      961 cgtggtcaag cggatccacg tggatatcgg ggagatggcg gtcgtgaacg ccgagcaggt
     1021 ggagttcctc ttccatgcca tcagcgagga cgaccccctg ttccgggggg ctgccctcgt
     1081 ctgcaggcca gtggtcccga ggggccgttg tgcctgcgga tacgaggggg acgaggtctt
     1141 tgtctgcccg gggtgcgggg cgatgcccga gctcgtccag gggagggaga tcgtggtcac
     1201 ctccctcgag atcgacgtgg acggcgcatg aaggtcaacc tcgcccacgg ggccggcggg
     1261 gagaccatgg gggagctcct gcggaccctc acgaacgtaa agaaccggaa cgccgggggg
     1321 atcggtctcg agtccctcga cgacggggcg gtgattcccc tcggcgacca gaacgtggtc
     1381 ttcaccaccg attcccacgt ggtccggccc atctttttcc ccggcgggga tatcgggagg
     1441 ctcgccgtct gcgggacggt gaacgacctc gcggttatgg ggggcaggcc catcgccctt
     1501 tcctgcggga tgatcctcga ggagggcttt gatatcatcg atctcgagcg gatcgtccgg
     1561 tccatggacg aggcccttac cgagtcgggg gcacacctcg tcacgggtga cacgaaggtc
     1621 gtggagcgcg gttccctcga taagatcatc atcaacaccg ccggaatcgg gatcgcagaa
     1681 aaaattgtcc gggacaatgg cttccggccc ggtgaccgga tcatcgtcac cgggaccctc
     1741 ggggatcacg gccttgccat cctcacccac cgggagggat tcgacctggg ggagcagatc
     1801 cgttccgacg tgtccccggt ctggggcctc gtggagatcg ccctcgccgc gggggacgtc
     1861 catgcgatga aggaccccac ccggggaggg tttgctcatg ccatcaacga catggcccgg
     1921 aagagccgga cgggggttgt cattgaggag gaggcccttc ccatccggag atcggtccgg
     1981 agcgcctcgg agatgctcgg gatcgatccc ctcgaggtcg cgaatgaggg gaagatcgtc
     2041 atggcggtgg caccggatga tgccggggcc gtcctctccg ggttgcggac gcacccgtac
     2101 ggaagggagg ccgccatcgt gggcacggtg gtcgaggggg accgggtcat catgcggacg
     2161 cgaatcgggg gagaacgctt catcgagcct cccctgggtg acccggtgcc gagggtatgc
     2221 tagacctgct tctcaggaac gttcaccttc ccgagggacg gaccgtcgac atttccatag
     2281 agaaggggat cgtggtccac gcgggggcgg gaggcagggc tgattcgacc cttgagtgct
     2341 cggaatttct cgtcctcccc gggggtgtgg acatgcatgt tcacatgagg gggggatccc
     2401 gggaatccag gaaggaggac tggggaaccg gcagccggtc tgccctcgct ggcggggtga
     2461 ccctcgctgt cgatcagccg aacacgaacc cccctctcac gacggtggag gccctccaag
     2521 cgcggatcgg ggaggccgga cgggactccc tctgccactt cgcggtgaat gccggtgtgg
     2581 tgccgggagc ggatctcccg gggcttcacc gtgcaggggc catggccttc ggggagatct
     2641 tcctcggtgc atcgacgggc gcaaaggggg tgtctggggg ggtactcggg ccgttactct
     2701 ccgggatcca cgccctgggt accaccgcga cccttcatgc agaagaggtc catgaggcag
     2761 atgacaccga tctcgtgtcc catgacgcga gccgccctca ggagggggaa atccgggcca
     2821 tcggaaaggt gaaatccagg accccgaagg gtatgcttct tcacttctgc cacctctcca
     2881 ccgcgggggc gctcgcggcc gccgcccccc acaccaccga ggtcaccccc caccacctcc
     2941 tcctctcccg ggaggatttc ccaaaggatg atccccgggg aaaggtgaac ccgcccctcc
     3001 gttcggccac cgagcgggaa gggcttcttg ccgcctggaa ccgggtcacg gtactcgcct
     3061 ccgaccatgc ccctcacacg agggaggaga aggcggggga attctcccgg gccccggcgg
     3121 gcgtgcccgg cgtggagacc atgatccccc tcatgatccc gagggtgctg tccggggaga
     3181 tgacccccgc ctccctcatc gaaaaaacct ccgggaaccc ctgcaggatc ctcgggatcc
     3241 cggtaccggg gttctcgggc ggtcatcggg ccgatttcgc cctttatccg agggagatca
     3301 ccacgatccg tgcgagggac ctccacagca ggtgcgggtg gacacccttc gaggggaggc
     3361 aggcggtgtt cccggacatc gtcatcctcg gcggcgagga ggttctccgg gagggcgatt
     3421 tcacccgggg gaatccccgc tggttcccgg gaaggggtta tcacccctga gcacccatac
     3481 cctatcgtgc tgatacagcg cacccatagg tccccgggtg agggctggtg tacgccttcc
     3541 ggtgatccac gggacaaccc gggcgcgcga caggcaccgc ccggcatgag gcgttaaaca
     3601 ggtgaaggac ggcaacagcc actggtgatc cctgtttgtt aatgccgggc gacaccttcc
     3661 tttctctccc gcgggtgtct ccatggaacc ctttgccgac gcggtgaccg aggatcagat
     3721 gggggtgatc ctggccctcg aagtgacccc cgcgagcgaa aggagcagtt tttttaccgg
     3781 ttacgatccc tggaggaaga gtatccggtg cacggtgcga tcccctccga gcaaagggaa
     3841 ggcgaaccgg gaggtgatag attccctcgc cggggcactc ggggtaccgg ccgcctctgt
     3901 caggatcctg tcgggcgcca cgcagcaccg gaagcgggtg aagatcgagg gaatcagccg
     3961 ggcggatgct ctcggacggc tcgatcgatt tctctgaaag agtggggtac ggcacggggc
     4021 ggggtcaggg actcccttac cccagaccgc ccatggaact ctttaaataa ttccaaaaga
     4081 tatatatttt tatacctatg ttcacgcttc gttcatcctg catgaatcat gggggagcct
     4141 tccctgaggg ggagggtgat tttacgtgta ctcatctgac acaaccaagt atctcattcg
     4201 catcaacctt cacgcggagg gggtggtcga gaaatctgat gtagtcgggg ccatcttcgg
     4261 ccagactgaa ggattactcg gcgaagacct ggatctgcgt gatctccaga ggaccggtcg
     4321 cgtcggacgc atcgatgtcc agatcgcgag caagaagggc gagacgaagg gcgagatcct
     4381 catagcctcg tccctcgacc gcgccgagac cgccatcctc gccgcctccc tcgagaccat
     4441 cgaccgggtg ggcccctgcg tcgcccatgt ctcggtggag aagatcgagg acatccgggt
     4501 cacgaagcgc aagatgatcg tggaccgggc caaggagctc cttatcgacc ggttcgacga
     4561 ggggtccatc gacagcaccg tgctcctcga tgacgtgagg gagtccctcc ggatcgagaa
     4621 ggtgagcgag atcgggccgg accatgtccc ggccggcccg aacgtcctcg agtccgatgc
     4681 catcatcatc gtcgagggcc gggccgatgt catcaccctc ctcaggtacg gtatcaagaa
     4741 cgccgtcgcc gtcgagggaa cgaaggtccc ggccaccatc gcggacctct gccagacgaa
     4801 gaccgcgacc tccttcctgg acggggaccg gggcggcgag ctcatcctcc gggagctcct
     4861 ccaggtggcg gacatcgact atgcggcctt cgccccgcgc gggagatcgg tggaggacct
     4921 ctcccggaag gaggtgacga aggccctccg gaacaaggtg cccgtcgagt tcgtgcggga
     4981 gcagctcgcc gagaaacctt cggaggtcag gccgccgaag gtgccgtcgc cggaggcaac
     5041 ccccgggatc cgggaatggg aggggactgc ccggaagcgg gaggccccgg ccaagggagg
     5101 gacgagcctg cgggaccatg tcgaggaggt ctcgggccac ggccttgccc ggttcatctc
     5161 gccggacttc tcggtcctcg cggaggtcag ggccggggat cttgagagga gcctttcggc
     5221 cataaagggc gacgttaccg ggatcgtcgc cgaccaggtc gtggaccaga ggctcctcga
     5281 ccaggtggcc cagaagggga tcgagttcat cctcgcccgg gatttcaaag ggatcatcaa
     5341 gcgcccgctt cccctgaagc tcatccggtt cgcccaggtg acctgaccgg cccctctcct
     5401 ttttccggac acggccgcgt actccggtgc aggaccctgc tttcatggct ctcctgtccg
     5461 gatttgccga agtttccgtc ggccggcctc tccgcggcgg ggctccgcgc cctgctggca
     5521 aggattcata tttcccggtc accaatgatg tatcaggata ccccccaccc acttctacga
     5581 ggccggacaa tgcacaagga agaactcatc tccctccacc agatgatggt ggagatcaag
     5641 gactttttcg agaagatcaa cccggagctc aagttctccc agtactacgc cctcaagatc
     5701 gacccctccc aggtccaccg gtcaaagatg gaacacaagt atgccatctt catcctcggg
     5761 accgagctcg cgaacgccat gcggaacgtc gagttcggcg catcggggag gatctcggcg
     5821 cggatgcggg agctcgccga gaagacgctg aaggagatcg agaacctcaa gtaacgggtt
     5881 ttccagcttc tttccacgct gttttctctt attcttgccc ggggattcat ggacgcttcg
     5941 gggggaggga ggtttcgtaa gagggaggtt tcgttagaaa ccgatctcga gcgacgcagt
     6001 cgcaagaaac cgacctcgag tgacggagtc gcgagttcgc tccgctcacc cccgccgctg
     6061 tggcggcccc cgcgtgggga taaccgtgta tggcatgccg tgaatttgcc gattttcgaa
     6121 taatcccatc cacctcctgc cggggctatc gcaactcggg ggttgccgaa gcggcagggg
     6181 catacgaggg gatgcatatc cccgagtatg gagcacccat tagggtgcga cgagtatagc
     6241 gagtcccccg agagcgtcca agtgatctat ggggatattc ttctgaaggg gagattccca
     6301 aagcctcatc ttccatgata cccgatgatc cgctcatgcg gtactactac gggagcgagg
     6361 aatccctcga ctatgccgag gtcaaccggg cggtggaggc ggtcttccgt gagcatgcgc
     6421 ggggacatgt ccagatgccc ccgaagatct atgtcaccct cgagcacggg gacttcagga
     6481 ccatgcccgc ctacgtgccg gccctcgaca tggcgggggt gaagatcgtg aacgttcacc
     6541 ccggcaaccg ggccgccggc ctccccacgg tcatggccct cacggtcatc ctcgacgtgg
     6601 agaccggggt cccgaaggcc atcatcaacg cgacccggct cacggacatg cggaccggtg
     6661 ccgcgggggc catcgccgcg aagtaccttg ccccgaagag ggaagtggtc ctcggggtca
     6721 tcggaagcgg gaggcaggcc gaggcccagg tccgggccat ctcccgggag ctcgtcatca
     6781 ccgaggtccg ggtctggtcg agggaggaga agaacgccct cgccctggcc gccatgttcg
     6841 gcgacctctc ggcctcggcc accaccatcc agaaggcctg cgatgccgac gtggtggtga
     6901 ccacgacccc ctctacgaaa ccaatcgtga tgcacgagtg gatccatgac gggacccaca
     6961 tcaatgccat cggggccgac gccccgggca aggaggagct cgaccccctc atcctgaaga
     7021 gggcaaaggt cttcgtggat gaccggcccc aggccattca ttccggggag atcaacgtcc
     7081 ccatcgcgaa aggcctcttc catgcccatg acatcgccgg gacgctcggg gaggtggtcg
     7141 tcggggtgaa ggggaggaaa cggcccgacg aggtcaccgt ctttgactcg accggcctcg
     7201 ccatccagga tctcgccatc gcgaacctgg caatgggggc cgggaaatgg gtcgacctcc
     7261 ccttcccctg accgtccccg cccttttccc ccggtcagtc ctctttccgg agcggcctcg
     7321 agtgcttccg ccagatctcg tcgcggtaca cggggccgct gttgtacgtg cagaagggga
     7381 tgatccgccc ctcgggggtg gcgtagtgga tgcagcaccg ctggacccgc gagaggtcgt
     7441 agttgtaccg gtccatgaag tgcatcgtgc cgatgaagag agagttccag tggaagtccc
     7501 gcagggcgtc gaagttctgg aagaggaggg cgcccgcgag aaccttccag atcttgggag
     7561 agcccgcgtt ctcccccttt ttcaccgagg tccggagggc gttcaccccc tcgaggaggg
     7621 tcaggtactt gttgatctgc ccgcctttcc tcaggttctc ggtcatcttc ttcaccgccg
     7681 cgaaaaactt gtcgacatcc accatccggt tgatgggcac catcccgttt tcggtgacaa
     7741 acacgtaggt cgcggccccg cagtgctggt gggcggtgaa ccggatctgg ggcttttcgg
     7801 tataggcctc cacgaggtcc gagatgggga caacgcaggg gaccgggtag aagtcctcct
     7861 tccgaattac gcccgaggtc tgggactcga tccgctccag gagctcgggg atggtgatcc
     7921 gtgcgttccg gacgtcgtcg tcgctcgccg cacccgtgaa ggccacgggc tggaagttca
     7981 ccccgcggac cacggagatg tgctccgcgg cgaaccggat gatgtccccc acctcatggt
     8041 cgttccggcc cttgatgatg gtcgggacga ggaccacccc gaggtggacc gagtcgagga
     8101 ccttcaccgc ctcccggtgc ctcccgatct ggggatcggt ctccctcgac accccgtcga
     8161 agtgcaggta cacggtggag agacccgcct ccttgagggc cttcgcatac ccctggtccc
     8221 tcgcgatctt caccccgttt gtcgcgatct ggacctgctt gaacccgagc tcctttgcct
     8281 tcctgatgat ctcgatcagg tcctcgcgga gggtgggctc cccgccggag aactgcacgg
     8341 ccggcgtggg gaccggcttc tcatcccgga ggagggcgag catccccgcc acctggtcga
     8401 aggtcggctc gtagacgtac ccgcacgcct gggcgttcac gaagcagaag tcgcagttca
     8461 ggttgcagcg gttcgtgagg tcgatgtttg cgaggagggt tcccgaccgg tggttcgagc
     8521 agacgccgca gtagcggggg cagccgtcct cgggggcctc ccggtgggga ttcgcgaggc
     8581 ccgtcccgag ggattcgaac tcgtcgaacc ggcggtagag ggcggcatcg gaccagtaga
     8641 ggtcacggaa ggggccgtcg gccgggcagg tccgctcgaa gaataccttg ccctcctcct
     8701 cgacgatctc ggcggggatg gtccggccgc agaccgggca gagagtccgt gtcgtcttga
     8761 gaagcaaaga atccgctccc gagaaccccg ttctattgtc ttttttattg ccttaatctt
     8821 ttttgctgca ttcttgccgg aacagccggc gttcagattg ctcttttcga taccttaatc
     8881 tttttcgcga cgctctatac taaactgttg gctggcatac tcactaccga cctctggttc
     8941 tccgcccttg tttctgccct ctggatcatg cttccggcct atattccgaa cccggccgcg
     9001 gtgattctcg ggggcgggag atccatcgac ggtggcgccg tctggaggga cggtcggaga
     9061 ctcttcgggg acgggaagac ggtgagggga tttgtcctcg gggtcctcgc aggggtggcg
     9121 gcagggggcg ccgggctctg ggtacaaggc ctcgccggcg gggccccggc ctggtcgttc
     9181 ctgccctccc atactcccct gaccgtctgc accttcgccc tgggggccct gctcggggac
     9241 ctcgccaaga gcttcgtgaa gcgccggctc gggaaggggc agggggatcc ctggccggtc
     9301 gccgaccagt atgacctggt cgccggggca ttcctcctca cggcccttct tgcccccgca
     9361 tggctcctcg caaccatcac cctccccgtc ctcgtctgga tcctcgtcct cacccccgtc
     9421 ctgcaccggg tgacgaacat ggccggccac cgcctggggg tgaagagggt cccatggtga
     9481 agcccctcgc ggccctcctc gctgcccacg gcgccgtccg gcacggagat tttctcctcg
     9541 cctcgggcgc ccggtcgagg gtgtacgtgg atatcaagtc cgccctcacc gagccggggg
     9601 tcctctcggt cctcgccctc gagatcgcgg gccgggggag cttcgaggcg gtggccgggg
     9661 tggcggtggg agggatcccc attgccgtcg ccgtatccct tgcggccgaa aaaccctacg
     9721 ccattgtccg gagcggggag aaggaccacg ggaccggcgg gaggatcatc ggcgatgtgg
     9781 ccgggcggtc cacgctcctc gtggaggatg tcaccacgac cggtgggtcg gtcctcgcgg
     9841 cggtccggct cctcagggcg gcgggggcga ggatcgacac ggtggtcacc atcgtggacc
     9901 gggaggaggg tgcggcgggg gccctcgcgg ccgagggagt gaccctcgtt tccctggtca
     9961 ccctcgcgga gctcctggcc ggaggctaac cttttaaccg acggggatga aaggatggtg
    10021 atgaagctcc tcctggtcgg cagcggggga agagagcatg ccatggccga ggccatcacg
    10081 agggacgggg aggcgaccct cgcggccttc atggcccgga gcaacccggg catcgcacgc
    10141 ctggcgagaa gcatccggat cgggaaggag accgacggga aaaacgtgag ggacttcgcc
    10201 gtggaggcgg gggtggacta cgccatcatc ggtccggagg cacccctgga ggcgggcgtg
    10261 gtcgacctcc tcgaagaggc ggggatatcc tgcgccggcc cgacccgggc agcggcacgc
    10321 ctggagaccg acaaggcctt ctgccgggac atgatggcgg cccaccggat cgacggctgc
    10381 ccgagatacc gggtcttcca cgatccggac ggggcggcgg ccttcatcga ggaccacgac
    10441 ggtgatctcg cggtgaagcc catcggcctc acgggaggga agggggtgcg ggtcatgggc
    10501 gagcacgtgg atcgcgcggg ggcggtggcg tacgcgagat ccctcggagg gaacgtggtt
    10561 ctcgaggagc gcctggtggg cgaggagttc accctccagg cctttgtcga tggaaaatcc
    10621 ctggttcccg cacccctcgt ccaggatcac aagagggcct tcgaggggga cacggggccg
    10681 aacaccggtg ggatgggctc ctactccctg cccgatcatc ggctgccctt cgtctccgag
    10741 gaggactacc ggagggctct ccggatcatg gaggatgccg tccgggccct cgcccgggag
    10801 gggttcccct accggggaat cctctacggc cagttcatga acacgaggga cggcccccgg
    10861 gtggtggagt tcaatgcccg gttcggggat cccgaggcga tgaacgtcct ccccatcatg
    10921 acgaccggcc tcgccgacgt ggtctgccgg atcatcgagg gaaccctctc ggcctcccgg
    10981 gtcggcttcg agcccgcggc gacggtgtgc aagtacgtgg tgcccgaagg ctacccggag
    11041 gcccccctga gcgcccagag ggtcaccctg ggggactccg ggagggcgcg gctctactac
    11101 gcgaacgtcg atgaaacgga cggatccctc gcgacccgga cctcccgcac cctcgccttc
    11161 gtcggtatcg ccggtaccct cgaggaagcg gagcgggcgg cggaggcggc cgcctcctcg
    11221 gtgaagggga gggtgtacca tcgcagggac atcgggaccc gggagctcct cgcccgacgg
    11281 tcggcccaca tggaggcgat ccgatgagaa gggatttcct ctcggtcctg gacatggatc
    11341 cccatgatct cacggggctc ctcgaccggg cggcccggat gaagaaggag agagggggag
    11401 aggaggaacg gcgcatcctc gcggggaagt gcctcgccat ggtctttgaa aaggcctcca
    11461 cccggacccg ggtctccttc gaggtcggca tggtggagct cggcgggcat gccatcttcc
    11521 tgaacccgaa ggatctccag ctcgggcggg gggaggagat ccgggacatg gcccgggtgc
    11581 tgtcacgcta tgtagcgggg gtcatgatcc gggccttccg gcacgaaacc atcgaggaat
    11641 ttgcccggca cgcctccatc ccggtggtga acggcctctc ggaccgggaa cacccgtgcc
    11701 agatcctcgg ggacctcctc accctcaggg agcacttcgg acggacggac gacctctccg
    11761 tcgcctggat cggggacggg aacaacgtct gccactccct catcctcgct tcagccctgg
    11821 caggcttcga gctcaccgtc gcctcgccgg cccgctaccg gccggaccgg ggaatcgtcg
    11881 agtctgcccg ggcggccggc ggacggatca ggctcgtcac cgatccccgg gaggcggccc
    11941 ggaacgccga tgccctctac accgacgtct gggtctccat gggagaggag gaggagcggg
    12001 agcagcggct cgccgccttc cgggggtaca ccgtcgacga gaacctcctc tccctcgcct
    12061 ctgacggggc cgtggtcttc cactgcctcc ccgcgcaccg gggggaggag atcaccgacg
    12121 cggtcctgga ggggccgcag agcatcgtct gggatcaggc agagaaccgg ctgcacaccg
    12181 agaaggccct tcttgcggcc ctcctcggct aggtggcccc ctcctttttc tcctcccgcc
    12241 tcgggaaccc tcccccggtc tcgatgaatt ggatcacctg ctccttcagg tatttttcga
    12301 ccttctccac cacctccctc tttccccgga aggccaggat ctcccgctcg gtcccgtcca
    12361 tgttcgcgaa gttcagggac atcttgcgtt cgacgagctt gacgtcgaac ttcctggaga
    12421 tgtcgatgat gatcgtcctc ggcacccctg gcgggatgat cacgtcatag agttcctctt
    12481 ctgccatcgg gaaaccctct ctctcacttc cctatcttca tccttctctt cataatacat
    12541 ggccctcccc ggacgccccc gagggggttc ctcggcttcc cgagggaaca catgcaccgg
    12601 cccatgaggg ccgccccccg gccgaggccg tcgtccacga aaaccaggtg gtcgatggga
    12661 tcatcgtaga tccctctctc ggcgatgcca tcgacaatca ggtacggttt attgccggag
    12721 atggcggccc gcccggtgaa cccgatggag gagtttggcg ggacgaggtt ctctcccctc
    12781 gcaacgtcca cgagccggag ggccatatcg gcacagaccc ggtccaccac ctcgtgaatc
    12841 gtggagagcc cgtggttttc gtagatctct gccccgatct ccacgagctt cgggatatcg
    12901 cccccgttct ccccgcagtc gcacccgatg agggcgaccc cggacttcgc agccacgtcg
    12961 gcatagacgg gcactctccc gaaccgcctc cggtccgggg ggaccatgcg gatatcgatg
    13021 tgcggccggc agcgttctac gtagtcctcg acgagagcag actccctggc ccggaacatg
    13081 cccgtggaag aggcctcccc gaagacatcc agggcgcacc cggtcctcgg atccacgagg
    13141 ccgctccccc ggacgagggc gtccgggatg gccccggcga ggccgcagaa gttaccgatg
    13201 gtctttgcga aggggttcac ggcatccttc gggacatcgc tcgtaatccg gccgtcgagg
    13261 gtcgtcccaa aatcgatggt gatgcagggg ttccggaagt ccacgggcgt ccacttcgcg
    13321 ccctccttga tccctgccat ggcgagctcc ccctccatct cgtttgccac catctcgacc
    13381 ccggtggaac cgaggggcgg gaggaccccg gcaaccgccc cgacaaagac caccttgtct
    13441 gtgaagctga agggctggag cttgagagag aggttctcct tggccatggg cgggcacatc
    13501 ttccgaggcg ggacaccggc gaggaggcag ccatttgcaa gtgcaatgac gaaatccccc
    13561 acctgatccg gagtttccat ctctgccaca acccccgtgg atcggacgac gaagtcgagg
    13621 tcacagttga tgtccaggtg gacctcccgg tggcactcga tgatgatgtc ccggacggcc
    13681 tcggtcaccg actgccgggt gatcggggtc ccgtcgatgg tctcgccaaa gatctcctca
    13741 cccggcttcg gcggcctgat atcccggctc atccgcaccg tcttgttgat gacgtaggcg
    13801 atgcctgtct cgagatccgt gcccgtgagg atgcacttcg tcgtggtatt tcccatctcc
    13861 accgaggcgc agatgaagta gggcttgaca cggtactcgg ggatgttcat tccggccccg
    13921 tgggcgatgg agggaggacg ggggctctcc acgatatggg gttttggcct gaagaaacgg
    13981 tcaaaaaaac gcgcagccat cggtataaag actcttcttc ctgattgtgt taaggttttc
    14041 tatgtgaaca ggggccggcg ggggaatgaa aggcctcttc ggggctggca gagacactat
    14101 accgcctggt ggtggtttcc ataacactaa taccttcgaa ggagactgct atccaccgga
    14161 gacatcggta cagccggatc ccgtaatccc gatatccgca gggagaacct gcctggagtt
    14221 cctgtgcccg gagaattcac cctcggcctg gtcgtggtcc tcattctggt cctcgccgcc
    14281 gagttcgtga acgggtggac cgatgcgccg aacgccatcg cgaccgtggt ctcgacccgg
    14341 gttctctcgc cgagaaaggc gatcgtgatg gcaaccctcc tcaacatcgc gggcgccttc
    14401 tcggggacgg cggtcgctgc aaccatcgga acgggcattg tcgatgcctc ggtgatcagc
    14461 ctcaccaccg tcgcggcggc catgatctcc ctcgtctcct ggagcacctt cgccgcccgg
    14521 ttcggcctcc cgacgagcga gagccatgcc ctggtcgcgg gcctcgccgg ggcaggcctc
    14581 gcggtggccg gccccgccgt cctcctctgg gaggggtggg agaaggtcct catcggcctc
    14641 gccttctcga ccttcctggg attcctgggg ggatggggaa tcaccatcgc catcaaccgg
    14701 gcctttttac attcccagcg gcggggaatg cgggagatct tcggcaggct ccagctcctc
    14761 tcggcggcct tcatggcctt cggccacggg agcaacgacg gccagaagtt catcggggcc
    14821 tttgtcctgg ccctggtcat cggggggatc cttcccgggt tccaggtacc tttctgggtg
    14881 gtcctcctct gctcggtgat gatgggcatc ggcacctcga ccggcgggtg gcgcatcatc
    14941 aggaccatgg ggatgaggat ggtgaagctg gaaccctacc agggttttgc cgccgagacg
    15001 ggagcggcga cggtcatcca ggctgcatcc gtcttcggca tccccctctc cacgacccat
    15061 accatcaata cgtccatcat gggggtgggg acggtgaagg gattctccgc ggtccggtgg
    15121 ggcgtcgtcc ggcagatcgt cctcgcctgg atcctgacgt tccccatctg cggtgccctc
    15181 agctggggca tcgccaccct cgtggtcagg gtcttgtagc ggggactctt cagatgaacc
    15241 gtggccttat cgagagaagg cgcggggtat agaggggccg gaagggtttc ccggcggtct
    15301 cgtcctttac cttcgtgacg agcccgtcct ccttcatcct ctcacggtgg agcttctcgg
    15361 gggtcgagag tttccggatg gttaccgtga gatcgcccga ggtgatctcc tggtccccca
    15421 tcacgacgat ataggggacc cactccatcc cggcggcccg gaccttcttg ttcatgctct
    15481 cctctctgtc gtccacatcg gcccggatcc cctgatcgtt caacctggtg cagagtgcga
    15541 gggccacatc cttgtgccga tccgcgacgg ggatgacccg gacctgagtg gccgagagcc
    15601 aggtgggaaa ctgggggacc tcctcgcccg cgagggtctc gagcatggcg cagatgaccc
    15661 gctccaccga gcccgtgggg gaacagtgga ggatgggcgg gtggatctcc tgcccgtcca
    15721 ggtgataccg gatcccgaac cgggtggagg attcgacatc gatctggacc gtcgggttct
    15781 cgatgggccg gccctgagcg tcgatggccg cgaggtcgat tttcgccacc cagtagtgga
    15841 cccgttcgga gaggatctcg atgagggtcg gctcaccgac gagacggacg accttcttca
    15901 cccaggcctc gtgctcctgg tagaagtccc gggtgcaccg gagggcagag tagtaccgca
    15961 cggcgaggtc ggtgccgctc ttctgcccca tgaccagctg ttcctcgaag caccggagcg
    16021 cctcgtccat gtccctgcag agggtgtgca tgtcgggcat cgtgaaggcc cggaggcgct
    16081 tcaggccgat gacctccccc ttctgctcgt gccggaagga gtacgtggag agctcgtaca
    16141 tcttcatggg gagggtgttc ggggagatgt gcatcccgtt catgatggaa aacatgccaa
    16201 agcaggccgc gaaccggagc atcatggagc ggttgccgct ccggaaccgg tactgccgct
    16261 ctccgaactt cgcggcatgc tcgcagatgg cctggtcccc gaggtcgtac atgaccggcg
    16321 tctcgacggg catggcgccg taggggagta cctgggtgag gacgtagtcg gcgatgaggt
    16381 cccggagaag cttccccttc ggcatccacc tgaggtggcc gacatcgctc ctcggctcgt
    16441 agtccacgag ctccttgctc cgcatgagct cgacgtgggg cggctcgccc gcggcaccga
    16501 cggggacccc gagctccttc ttcacgaggc agccgaaggg ggactcgtcc atgtaatcct
    16561 tgacgtcctt ccgcttccca tccggggtga ggatgaacca ctcgtgctcc acctccttct
    16621 ttgagacggc ctcctccttc tccccgccgg cgactaacgt ccgggaaagc tcggagaggg
    16681 ggtggccctt gcaggtgagg gtaaaggcct tgtaccaccc gaagggggcc ctctttacct
    16741 cgaggcccag gtcggagagg ccctgccgca cggcctcgag ggcctcgacc gcggcccccg
    16801 cggtcgcgag gtcgctcgcc aggtgggcgt aggggtacac catgaccctt cggcagtcca
    16861 gctgggaggc ggtcttcgcg atgtccgtga ccgcctgctc caccacgtcc cccatgtcct
    16921 cctcgtccgc cgcctcaacg gcgcagaagg ccacgagggc ctcgtccatg gagtcccgga
    16981 ggttctttgc atcctctgcc atggaggtgg gtttctttgc ctcgtaatcg atgtgatcgg
    17041 agtggatgag gagaaggcgc atggtgtggc tcctgtttta cctcggaatg ctaccagtat
    17101 tcgcgctgcc tgcctttaaa tatcactcat ccgtgtcc
//
LOCUS       DATGDI010000044         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_149702, whole genome shotgun sequence.
ACCESSION   DATGDI010000044 DATGDI010000000
VERSION     DATGDI010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149702"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1820
                     /locus_tag="VKO45_01710"
     CDS             183..1820
                     /locus_tag="VKO45_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HMA04620.1"
                     /translation="MRIATACLVCLICMSGLAIHATAQEVVVDPVTVTADLAPGAVQN
                     VQINVTFPGSVPRGDVLFAFDATGSMGPVLDAMRNQGIQVMNDIRAVIEDTRFGALSF
                     MDYPKHFDNYYGYSNTYGAEGDYDFLLDQGLTPDINAVSAAITRMNLGDGGDIPEDYG
                     RAIYEARDLPWRTDAKKILVVFGDAPPHAAPGGKTLMNPWSTGLLFTSTEMSAPYGGD
                     PGRDMAPSGDDIDYATQIQGAADDYISIVAVYCPYNGVLDDSHRDAENNFRYMAYKTG
                     GPFVLINPNGDASLIADEIVSMIQAMAKQNIRELSLRVEEPGYAGWVTTPDSYSDVPW
                     PSEKAFQVAISPPADAVGGDHTFHLSIVGDGIVLGSVTVSVHVDIPDTTPPVEVSLDI
                     RPRDCPNSFNPGEKGVLPVAILGTDALKVSAIDRTSIRLSREGGSAWVGSIRTAVEDV
                     SSPSTGSCSCGLVSGKKDKVPDLALKFDSQELVQELGIGKDEGCIKLTVTGTFVSDTG
                     VPSQRFIGTDYLRVIETGKGSCAGDGGNEGKGPGNQG"
     gene            complement(1882..2517)
                     /locus_tag="VKO45_01715"
     CDS             complement(1882..2517)
                     /locus_tag="VKO45_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04621.1"
                     /translation="MMDRILVWVIGILAALCIIVAGVVILLAPVHGGPVIRSEAEAGC
                     LASGGSVGEGLCCLSVDDFPDMCLVGACGCSAENSHAVQTCTCPGGMCFNGTACASLI
                     TSFDECVAAGYPVMESYPRQCRVPGGPTFVEQVPPAITAASCQAARGHWKECGSRCQI
                     DNAGKTGIACPALCEALCECGGIAGFGCPAGYTCRMPEGIADALGYCVSTG"
BASE COUNT          554 a          838 c          832 g          485 t
ORIGIN      
        1 cagaagtcga taataacaga cattagaata gggaatctat atatactata agatagaatt
       61 gtcttcctga tcaagaagta tccacagcag ctggttggtt ccaaccggcc accatcgaat
      121 cgccggaagg gacccggtgg ccggaatccg gcagcggtgg atagcatacg ggagggagaa
      181 acatgaggat agccacggca tgcctcgtat gcctgatctg catgtcaggc ctcgcgatac
      241 acgccaccgc gcaggaggtg gtggttgatc cggtcaccgt cactgcggac ctcgcaccgg
      301 gtgcggtaca gaatgtccag atcaacgtga cgttcccggg aagtgttccc cggggggacg
      361 tcctctttgc cttcgatgcc acggggagca tgggaccggt gctggatgcc atgaggaacc
      421 agggcatcca ggtcatgaac gacatccggg ccgtcatcga ggacacgcgt ttcggggcgc
      481 tgtccttcat ggactatccc aagcacttcg ataattatta cgggtactca aacacctacg
      541 gggcggaagg ggactacgac ttcctcctcg atcagggcct tacccccgat atcaatgcgg
      601 tatcagcggc tatcaccagg atgaacctgg gggacggtgg agacatcccc gaggattatg
      661 gccgggctat ttacgaggcc cgtgaccttc cctggaggac cgacgcgaag aagattctcg
      721 tcgttttcgg ggacgctccc ccccacgcgg cccccggcgg taaaactctc atgaacccct
      781 ggtcgacagg ccttctcttc acgagcacgg agatgagtgc gccctacggg ggagaccccg
      841 ggagggacat ggctccatcg ggggatgaca tcgactacgc tacccagatc cagggggcgg
      901 ccgacgacta tatctccatc gtggctgtct attgccccta caacggggtt ctcgacgatt
      961 cccaccggga cgcagaaaac aacttcaggt acatggccta caagaccggc gggcccttcg
     1021 tgctgataaa cccgaacggg gatgcctccc tcatcgctga cgagatcgtt tccatgatcc
     1081 aggcaatggc aaaacagaac atccgggaac tctccctccg cgtggaggag cccgggtacg
     1141 ccggatgggt gaccaccccc gattcgtact ccgatgtccc gtggccttcc gagaaggcat
     1201 tccaggtggc catctcaccg cctgccgatg ctgttggtgg cgaccacacc ttccacctca
     1261 gtattgtcgg tgacgggatc gtgctcggca gtgttacggt gtcggtgcat gtggacatcc
     1321 cggacacgac ccccccggtt gaggtgtccc tcgacatcag gccacgggat tgtccgaact
     1381 ccttcaaccc gggggagaag ggggttctac ccgtggcgat cctcgggact gacgccctga
     1441 aggtctccgc catcgacagg accagtatca ggctctcaag ggagggcggg tcggcgtggg
     1501 tagggtcgat ccggaccgca gtggaggatg tatcctcacc ctcgacgggc tcgtgttcgt
     1561 gcggcctcgt ctcgggcaag aaggacaaag tgccggatct tgctctgaag ttcgattccc
     1621 aggaacttgt gcaggagctc gggatcggga aagacgaggg ctgcatcaag ctaaccgtca
     1681 cgggaacctt cgtgagcgat acaggggttc caagccagcg gttcatcgga acggactacc
     1741 tgcgggtgat agaaacaggg aagggctcct gcgccggcga tggcgggaat gagggaaagg
     1801 gacccggcaa ccaggggtga caccggagca ggtcagggcc ggaatcaccc cgctttcagg
     1861 aaggcttagc ccacggttgc cctatccggt ggaaacacag tacccgaggg cgtccgcgat
     1921 cccttccggc atcctgcagg tgtagccagc cgggcacccg aatcccgcga tccccccgca
     1981 ctcgcagagt gcctcgcaga gcgcggggca ggcgatgccc gtctttcccg cattatcgat
     2041 ctggcatcgg ctcccgcact ccttccagtg gcctctcgcg gcctgacagc tcgcggccgt
     2101 gatggcgggc gggacctgct ccacgaaggt cggtccgcct ggaacccggc attgccgggg
     2161 gtagctctcc atcacaggat aaccggccgc cacacactcg tcgaagctgg tgataaggct
     2221 cgcgcaggct gtcccgttga agcacatgcc ccccgggcag gtgcaggttt gcaccgcatg
     2281 gctgttctcg gccgagcatc cgcaggcccc gacgaggcac atgtccggga agtcatccac
     2341 cgagaggcag cagaggccct ctcccacgga tccccccgag gcgaggcagc ctgcctcggc
     2401 ctcgctcctg atcaccgggc ctccgtgcac cggggcgagg aggatcacga cgcccgcgac
     2461 gataatgcag agggccgcga ggattccgat aacccagaca aggatcctgt ccatcatcca
     2521 tcaccgcgaa agagtggaac gaggggatga tattcgtttc gcaggcagaa ggaaccaccc
     2581 ggagtcatgc cctcagcacc tcctgatgcc ccgtaccgct tcccggatgg catccgcatg
     2641 gtccccatcg gtatccagcg tgaagaggat attcctgtcg tcggtttcat agattcccag
     2701 ccgatagct
//
LOCUS       DATGDI010000045         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_207934, whole genome shotgun sequence.
ACCESSION   DATGDI010000045 DATGDI010000000
VERSION     DATGDI010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207934"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VKO45_01720"
     CDS             <1..918
                     /locus_tag="VKO45_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HMA04622.1"
                     /translation="YMGTGEGGLHQALYPYQDRMIVQVASGRFGVDINYLERGAAIEI
                     KIGQGAKPGLGGHLPGEKVDADVSSTRMIPVGSDAISPSPHHDIYSIEDLKQLVRSLK
                     EATEWKKPVFVKIAAVHNSAAIAAGIARSSADAVVLDGFRGGTGAAPKVFRDHVGIPI
                     EAAVASVDAKLRQQGIRNEISIIASGGIRESADVAKAIALGADAVYIGTAALVALGCR
                     VCGNCYRGLCPWGIATQREDLVARLDPETGSRQAANLINAWTLELSELMGAAGINSIE
                     SLRGNRDRLRGYLLDEGILKVLDVKTVGA"
     gene            920..1660
                     /locus_tag="VKO45_01725"
     CDS             920..1660
                     /locus_tag="VKO45_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04623.1"
                     /translation="MPPYRIDAESLSYTRLNQLLRAAVAAGEKEIEIDHVLGQRFIAD
                     GLKGEVTITIHGVPGGDLGMFMSGPTCIVHGNAEHAPGNTMDAGMIVIHGSAGDAVAH
                     SMRGGKVFVRNDIGYRGGIHMKEYNSKRPTLVVGGSARAFLGEYMAGGLIVILGLDGR
                     PAVTERGIGSGIHGGEIYVREDFPDHLLGVGATKKPAEAPERERIAPYLREFAGHFGM
                     DAAALISGEYVRIAPASSRPFASKYTWE"
     gene            1664..>2109
                     /locus_tag="VKO45_01730"
     CDS             1664..>2109
                     /locus_tag="VKO45_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase/dehydrogenase beta
                     subunit N-terminal domain-containing protein"
                     /protein_id="HMA04624.1"
                     /translation="MSGKSYRDLKRDVWDAGLCSGCGACVAVCPAEGLYFEAGKGGAS
                     PLSTGTCKEDLDSVPCGVCIEVCPRASGQTREMLGEYSDALAARASFEIPRRQSGGAV
                     TAILANALDQGLLDGVVTVTEERFTLEPRSAVITSSEVLIHEAGSR"
BASE COUNT          369 a          657 c          775 g          308 t
ORIGIN      
        1 tacatgggaa cgggtgaagg gggattacac caggcccttt acccgtacca ggaccggatg
       61 atcgtccagg tggcatcggg gaggttcggc gtcgatatca actacctcga gaggggggcg
      121 gccatcgaga tcaagatcgg ccaaggagcg aagcctggcc tcggtgggca cctcccgggc
      181 gagaaggtgg acgcggatgt ctcctccacc cggatgatcc ccgtcgggtc ggatgccatc
      241 agcccctccc cgcaccatga catctacagc atcgaagacc tgaagcagct cgtgcggtcc
      301 ctgaaggagg ccaccgagtg gaaaaagccg gtctttgtga agatcgccgc ggtgcacaac
      361 tccgccgcca tcgccgcggg gatcgcccgc tcctcggcgg acgcggtggt cctcgatggc
      421 ttccgtggcg ggaccggggc cgcgcccaag gtcttccggg accatgtcgg gatccccatc
      481 gaggccgccg tcgcgagcgt ggacgcgaag ctcaggcagc agggcatcag gaacgagatc
      541 tccatcatcg cgagcggggg gatccgggag agcgcagacg tggcgaaggc catcgccctc
      601 ggggcggacg cggtctacat cggcaccgcg gccctcgtcg ccctcgggtg ccgggtctgc
      661 gggaactgct accggggcct ctgcccctgg gggatcgcaa cccagcgcga ggatctcgtg
      721 gcgcggctgg acccggagac cgggtcccgg caggcggcga acctcatcaa cgcctggacc
      781 ctggagctct ccgagctcat gggggcggcc gggatcaaca gcatcgagag cctccggggg
      841 aaccgggacc ggctgcgggg gtacctcctg gacgagggga tcctgaaggt gctcgatgtg
      901 aagaccgtgg gggcatgaga tgccgccgta ccggatcgat gcagagagcc tctcctacac
      961 ccggctgaac cagctcctgc gtgccgccgt ggcggcgggg gagaaggaga tcgagatcga
     1021 ccatgtgctc ggccagcggt tcatcgccga cggcctgaag ggagaggtga ccatcaccat
     1081 ccatggcgta cccggggggg acctgggcat gttcatgtca ggtccgacct gcatcgtgca
     1141 cggcaacgcc gagcatgcgc cggggaacac catggacgcc gggatgatcg tcatccacgg
     1201 gtcggcgggc gacgccgtgg ctcactccat gcggggcggg aaggtctttg tccggaacga
     1261 catcgggtac cggggcggga tccacatgaa ggagtacaac tcgaaacggc cgaccctggt
     1321 cgtcggaggg agcgcccggg cattcctcgg tgagtacatg gcgggagggc tcatcgtcat
     1381 cctcgggctg gacgggaggc cggcggtgac cgagaggggg atcgggagcg ggatccatgg
     1441 cggggaaatc tatgtcaggg aggacttccc cgaccacctc ctcggggtgg gggccaccaa
     1501 gaagccggca gaggccccgg agcgggagcg gatcgccccc tatctccggg agttcgccgg
     1561 gcatttcggg atggatgcag cggcgctcat ctccggggag tacgtgcgca tcgccccggc
     1621 gagcagcagg ccctttgcca gcaagtatac gtgggagtga accatgagcg ggaaaagcta
     1681 ccgggacctg aagagggatg tctgggacgc cggcctctgc tccggctgcg gggcatgcgt
     1741 cgcggtctgt cccgccgagg ggctttactt cgaggcaggg aaggggggcg cgagcccact
     1801 ctcgaccgga acctgcaagg aggacctgga cagcgttccc tgcggcgtct gcatcgaggt
     1861 ctgcccgagg gcctcgggcc agacgcgtga gatgctcggg gagtactcgg acgccctcgc
     1921 cgcacgggcc tccttcgaga ttccccgacg gcagagcggc ggggcggtga cggcgatcct
     1981 cgcaaatgcc ctcgaccagg ggctcctcga cggggtggtg acggtgaccg aggaacggtt
     2041 caccctcgag ccgagatcgg cggtcatcac ctcctcggag gtcctcatcc acgaggcggg
     2101 atcgcgcta
//
LOCUS       DATGDI010000046        12814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_62654, whole genome shotgun sequence.
ACCESSION   DATGDI010000046 DATGDI010000000
VERSION     DATGDI010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 12814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12814
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62654"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VKO45_01735"
     CDS             complement(<1..826)
                     /locus_tag="VKO45_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04625.1"
                     /translation="MIRESLREALAHLAGTPVIWITGLYLGAFFAADILLQVSGSTLV
                     ASRAGFLGLVAAPFFLGGTYGVIRGDGSGLRAYLTAGSRYFFRILLAGAVTVSVALFT
                     ALLVMLPILIVGGSLQAAIPSALLGVGVSFAFFTAFVDTAVVFEDRKVLDSIRRSVEF
                     VTGNLKTVLGFYLANLAIGFAVFFVSVVIWSFTIADRLLPLVEGNQTVIQNLTAPDMV
                     SLVGLPGLMAGVTIGFFAITFGATLLVAFKACYFRRVAASTPAPVAQGEFDEKGRWY"
     gene            complement(830..1420)
                     /locus_tag="VKO45_01740"
     CDS             complement(830..1420)
                     /locus_tag="VKO45_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HMA04626.1"
                     /translation="MSYTRRDIYYFDRPGGENTRDAARMAVERAKALGIRQIVVASTG
                     GSTALAFLEAVKGNGIELVVVTHVVGFEKPGVWEFSEEAAKTLRAAGARIVTGTHALS
                     GLERALSRAPRVGGGSRTEAIAEALRRIVAMGLKVAVECCLIAADQGAIPVDEEVVAA
                     GGTMSGADTVCVIRPAHTASFFDLQVREIVAMPRNR"
     gene            1794..2963
                     /locus_tag="VKO45_01745"
     CDS             1794..2963
                     /locus_tag="VKO45_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HMA04627.1"
                     /translation="MITLALAGKPNCGKSTFFKAATLANVEIANYPFTTIDANHGVGY
                     VRSPCVCRELGTTCGVCRDGVRFIPIGLIDVAGLVPEAHKGRGLGNQFLDHLRQADAI
                     LHLLDASGSTDAEGNPVGAGSHDPAGDIEFLEYEMSMWLYGILEKHWTKIQRQSQAKT
                     SSMAHAVAELIAGLGITAEQVRAVELETGIDLVRAPEPELVRFCRRLLTVAKPMMIVG
                     NRVDEAPDPLVEGLRARGIRFATAAGELALRNAASAGIVKYLPGDPSFSILKPEILNP
                     PQRAGLERIAGVMKKFGGTGVQEALNHAVFSLLDLIVVYPVEDEHKYADGKGRVLPDA
                     FLLKKGSTSRDLAFRVHTEIGEGFLYAVDARTKMRVKDTHPLKDRDVIKIVSTRK"
     gene            3071..3391
                     /locus_tag="VKO45_01750"
     CDS             3071..3391
                     /locus_tag="VKO45_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04628.1"
                     /translation="MGAEIYQAQVIRNFFETITGPDRNLTRIYMCVISLAKLRMEAPE
                     KQKELLDQLRKSREKKELSVDILDYVCSAAIELEITTVQTAFGVTEIAKAVQDFEKVS
                     FDSL"
     gene            complement(3439..3906)
                     /locus_tag="VKO45_01755"
     CDS             complement(3439..3906)
                     /locus_tag="VKO45_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04629.1"
                     /translation="MDRYLTEYIERVRPDFSGIPEAAGHQLAFVFLSFKLGIYHDAVT
                     RCEQALKLIGGIPVPTVLGVALRIVRERAEALTQAQYDARLPFLFTKADLPFGIIDPS
                     EPVVDADLFQLTNALIIIYAAALASSPDDEQALEEQEKYVLQLLATYRKSLNR"
     gene            complement(3929..4264)
                     /locus_tag="VKO45_01760"
     CDS             complement(3929..4264)
                     /locus_tag="VKO45_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04630.1"
                     /translation="MAEGSFPAFIKGRLHPWMLVPGLAWGGIVAGFLLSPPSLLPGSA
                     GCFLGSLAIAGLALTRTKKDIVSLCTPIFALLIFVFPLETPPGLAMQLLFAATLTGLL
                     IRLERRFPP"
     gene            complement(4373..5683)
                     /locus_tag="VKO45_01765"
     CDS             complement(4373..5683)
                     /locus_tag="VKO45_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04631.1"
                     /translation="MFRFLKGRRKADEAPAPLGIDDLPPWLDAEESRIRADLSARVAG
                     HRPAVLKARGRMEGVLSGFDAASMEEVSSLKLAGVTERSLPLFLRAMRMSLSRDLPDD
                     PEGFYTAAGEILKGCLSAFRGQGRYLASRFPAEMKVLRDGVDDIGREVNSLTPEVGRA
                     RERLRELAGLRAALELHGGERERLAAARNGIREREEEVADSRRSLEAAVRARGELEKG
                     EDHREFEKELLRIRGLEGEHADLERRFREASATAIHLLRKGEKIASRRKDREALKAIR
                     ETGDLLEGGLPPGGEAALAILPAGQKALAAMAASGELVPKNREEIDLIERPGDLVHRI
                     TSITRRYREISEEMDSAQVALRARPVSVKRTELMKEAEELEKRLARAGEGLDRAREEV
                     STLDKRMQASLAEVSRRAEALSGRPLRTREPDSAEAEGRAGRSG"
     gene            complement(5703..6209)
                     /locus_tag="VKO45_01770"
     CDS             complement(5703..6209)
                     /locus_tag="VKO45_01770"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HMA04632.1"
                     /translation="MTRGFYIGRFQPYHNGHQAVLERVAGEVDEIVIGVGSAQLSHEL
                     ENPFTAGERVLMITRSLASLDRPLYVIPIEDIQRNALWVSHVRSMTPPFDCVFSNNPL
                     VMRLFAESGSTVHCPDMYERQTHSGTAIRSRILAGEPWEHLVPRAVVEVVREIDGVER
                     LRQIARSD"
     gene            complement(6206..6982)
                     /gene="larB"
                     /locus_tag="VKO45_01775"
     CDS             complement(6206..6982)
                     /gene="larB"
                     /locus_tag="VKO45_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HMA04633.1"
                     /translation="MQPHANLLGLLEAYRRGELTREEAASRIEGLRIEQLGEYARLDL
                     GRAARCGVPEVILAEGKAPAQLAEITVRAVAAQGRCIVTRVTPDQAEEIRRRCGEEGF
                     QAEHLEVPRAIVAFRLPVQEKTGGIVAILTAGTADLPAAEEAKLIAEEMGCEVRTAYD
                     VGAAGIHRLFPALKSVLDAHVFIVAAGREGTLPAVVAGLVDRPVVGLPVSSGYGYMGE
                     GRAALASMLQSCSVLAVVNIDAGFTAGAFAARIAAMVAGL"
     gene            7030..7350
                     /locus_tag="VKO45_01780"
     CDS             7030..7350
                     /locus_tag="VKO45_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA04634.1"
                     /translation="MSYLIFVAGFGALATYLLWFRDVRIFFRTGLPGYRAAARHGVAY
                     ATLALIGVDVGLIFDEVVGLGLILLALYLQGREKREKVFSGGEPALDRLLGKTAQRKD
                     KERE"
     gene            7415..8647
                     /gene="hisS"
                     /locus_tag="VKO45_01785"
     CDS             7415..8647
                     /gene="hisS"
                     /locus_tag="VKO45_01785"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HMA04635.1"
                     /translation="MLQRPRGTRDFLPEEMERRRSVEVCLRERVARWGYREVCTPEFE
                     ELELFTLRSGAGIVGEIYAFEDKGGRKMALRPEITAPVLRMFVSEAKVHPRPLRWFYF
                     ADCFRYERPQKGRYRQFWQFGTELIGADTPRADAETILLADDLLRAAGIPCDLHVGHL
                     APMKHLLADLDPAAQAKAMAFLDKRDFDGLTSLLAGSGRDDLVDTLRALAHARTLPEL
                     FGITGEIPERRRIEETVEILEKNHLVYTLDFGIARGLDYYTGVVFEGFAPNLGAENQV
                     LGGGAYRLAHLFGGDDVPSCGFAIGFDRLMVSLGEMEMPRPVRVAVCSTEEGRIRAFE
                     VAKAFRDGGVSAEIDLLDRNLGAQIAHAARSADYVAILGKREVETGTVTLKDLQTGEQ
                     KAVPLPDAVAEVAARVPR"
     gene            8634..10436
                     /locus_tag="VKO45_01790"
     CDS             8634..10436
                     /locus_tag="VKO45_01790"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HMA04636.1"
                     /translation="MSLAEELARKQRAISIAEFFEKNKHLLGFDSPTRGIITTVKEAV
                     DNALDACEEAQALPDILVQVKKISDEVFRVTVEDNGPGIVPAQVPFVFGKLLYGSRFH
                     QIKQSRGQQGIGISAAVLYAQLTTGVPSLVISRTDAKKPASRFELAIKIESNEPEVVS
                     QEEVEWDRTHGTRVEIELRATLAAKKRLIDYLKYTSVVNPHARIRAEIDGEVYTFDRV
                     SHDPIPLPCPIQPHPHGIEIGLLKRMAAESPLPLEGFLVESFSRVGKKAAEEILRKAG
                     LSGGKKAKSLTNEEQKSLLAAMQAVAIPAPPTTQCLSPIGEELIRKSLDKEFQLDFVK
                     AKTRPPSVYSGHPFVVEAAIGYGGRLASEEKAIILRFANRVPLMYQQGACAITQAIAG
                     VNWRNYGISQDGLPVGPALILVHVASTNVPFTSESKDAVAAIPEIEKEIDLALKDLGR
                     ELKTFLSRRDRHRVQEDRARALCAIIPELAEKVAEVTGKPVPDITPIEGRIMRRLVAK
                     KWTSDGQVRIAVRNYTDKEISPTVYDTSPDPAPDAEPKPGYTHAVEKDFTRVWKLTLA
                     PGEGWEAKYTGAGGGTLDIQGVEEAVKLVVDLDV"
     gene            10429..11517
                     /locus_tag="VKO45_01795"
     CDS             10429..11517
                     /locus_tag="VKO45_01795"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HMA04637.1"
                     /translation="MSKEEFDSLARTRLLAIALEWYNEMQGGEIPYIRLPTRTKANIE
                     YDSGSEVWKYGDRESLRAATSEKGALHLLKMAYVIGFIKQQLAENRSSTLRELYYISE
                     GWKRAKFGAQDESNLLIEDLEIITDLQREAFHLRPEEDGATVFGPLRIREKTRRGVRD
                     IHCRDDVGEAGYQIPNDVETVEFLEHDAKFVIALETGGMYARLIENGFDEEHQAIVVH
                     LKGQPARSTRRILHRLNEQFGLPITVFTDGDPWSYRIFASVAYGAIKSAHISELLATP
                     KAQFIGVQPTDIRDYNLPSDGLTDKDVDALRAELSDPRFDSEYWKGQINLQLEMKLKS
                     EQQAFAARGLGFVTKEYLPSRLSEMGVI"
     gene            complement(11574..12506)
                     /locus_tag="VKO45_01800"
     CDS             complement(11574..12506)
                     /locus_tag="VKO45_01800"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMA04638.1"
                     /translation="MRVLVTGAGGLAGRAMVRQILGTKGDHEVLGVYRRNLPPPSDRF
                     TPVKLDLADREKTLSRVKEAGPGAIIHLAGLNHGTMAELLESNVIATQNILDAACTLG
                     APVRVLVTGSSAEYGYAGSGPIPESALLRPVGPYGVSKAAADLLAQSYGAVRHLPVAV
                     IRPFNLVGPGQSDAYVCGKIVRQVTEIDEGKREEISLSGVSSRRDFVDVRDLAVACWD
                     LVTLPEFPERCTGKAFNVGSGKDSSVAEVLEMVRRITGKRHPVLLPEKEMPVPIPSQR
                     SDNSLITATCGWRPVISLTQSLKDMLAFERGRVR"
     gene            complement(12503..>12814)
                     /locus_tag="VKO45_01805"
     CDS             complement(12503..>12814)
                     /locus_tag="VKO45_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMA04639.1"
                     /translation="HREVNVDFGVLEPDSEGRIRAFREKPTYPFEVSMGIYAFQREIL
                     GFVPEGRPFGFDELMLAMIAGDAPVYSYPHPGYWLDIGRPDDYERSIVEFERYRDLFL
                     G"
BASE COUNT         2351 a         4204 c         4202 g         2057 t
ORIGIN      
        1 ggtaccacct ccccttctca tcgaattcgc cctgagcgac cggcgcaggg gtggatgccg
       61 ccacgcggcg gaagtaacag gccttgaagg cgacgaggag ggtcgccccg aaggtaatcg
      121 cgaaaaaccc gatggtcacc ccggccatga gccccggcaa cccgacgaga ctcaccatgt
      181 cgggggcggt caggttctgg atcaccgtct ggtttccctc cacgagggga aggagccggt
      241 cggcgatggt aaaggaccag atcacgacgg agacgaagaa gacggcgaaa ccgatggcga
      301 ggttcgccag gtaaaacccg aggacggtct tcaggttccc ggttacgaac tccaccgatc
      361 gccggatgga atcgagtacc ttccggtcct cgaagaccac cgccgtgtcg acaaaggcgg
      421 tgaaaaaggc gaaggagact ccgactccca ggagggcgga ggggatggcg gcctggaggg
      481 atccgccgac gatcaggatg gggagcatga cgaggagggc cgtgaagagg gccacgctca
      541 cggtaaccgc ccccgcgaga aggatccgga agaagtaccg ggaacctgcg gtaaggtagg
      601 cgcggaggcc cgacccgtcg ccccggatca cgccataggt ccctcccagg aagaagggtg
      661 cggccaccag gcccaggaat cctgcccggg aggcgacaag ggtgctcccg ctgacctgca
      721 gaaggatgtc ggcagcaaag aaggccccca ggtagaggcc ggtgatccag atcacggggg
      781 tgcccgcgag atgggcgagg gcctccctca acgattccct gatcatgctc taccggttcc
      841 tcggcatcgc cacgatctca cgtacctgca ggtcgaaaaa ggaggcggtg tgggccggcc
      901 ggatgacaca gacggtgtcg gccccgctca tcgtgccccc tgccgcgacg acctcctcgt
      961 cgacggggat ggctccctgg tcagcggcga tgaggcagca ctccaccgcc accttgaggc
     1021 ccatcgcgac gatgcgccgg agcgcctcgg cgatagcctc ggtcctcgac ccgcctccga
     1081 cccgcggcgc ccgggagagg gcgcgctcga ggccggagag ggcgtgggtg ccggtgacga
     1141 ttctcgcccc cgcggcccgg agggtcttcg ctgcctcctc cgagaattcc cagacgccgg
     1201 gcttctcgaa gccgactacg tgggtgacca ccacaagttc gatcccgttt cctttcacgg
     1261 cctccaggaa ggcaagagcc gtggaaccgc ccgtggacgc gacgacgatc tgccggatcc
     1321 cgagtgcctt cgcccgctcc acggccatcc gggccgcgtc ccgggtgttc tccccgccgg
     1381 gccgatcaaa ataataaata tcccttctcg tgtaactcat ggtacctagt acgagcgttt
     1441 tgcatcatta aaggggattg gtatgatcaa gctggccctt gcgggaaaac ccaactgcgg
     1501 aaaatccacg ttcttcaagg ccgccacaca ggaggttata accgtatgat cactcttgcc
     1561 ctcgcgggaa atccaactgc cagaaatcga cgttcttcaa ggccgccaca caggaggttt
     1621 taaccgtatg atcactcttg cccttgcagg gaagcccaat tgtggaaaat caaccttttt
     1681 caaggccgcc acgtgggagg atgaaacctc atgatcacac tggccctggc gggaaaaccg
     1741 aactgcggca aatcaacctt cttcaaggcc gccacgtggg aggatgaaac ctcatgatca
     1801 cgctggctct ggcagggaaa ccgaactgcg gaaaatcgac ctttttcaag gcggcgaccc
     1861 tggcgaacgt cgagatcgcg aactacccct tcacgaccat cgacgcgaac cacggggtgg
     1921 gctatgtccg gtccccgtgc gtctgccggg agctcgggac gacctgcggg gtctgccggg
     1981 acggggtgcg gttcatcccc atcggcctca tcgatgtcgc gggcctcgtc ccggaggccc
     2041 acaaggggcg cggcctcggg aaccagttcc tcgaccacct gaggcaggca gacgccatcc
     2101 tccacctcct ggacgcgagc ggctccacgg atgccgaggg gaacccggtc ggggccggct
     2161 cccacgatcc tgccggggac atcgagttcc tcgagtacga gatgtccatg tggctctacg
     2221 ggatcctcga gaagcactgg acgaagatcc agcggcagtc ccaggcgaag acctcctcca
     2281 tggcccatgc cgtcgccgag ctcatcgccg gcctgggcat caccgccgag caggtgaggg
     2341 cggtggagct ggagaccggg atcgaccttg tccgggctcc ggagccggaa ctcgtccggt
     2401 tctgccggag gctccttacg gtggcgaaac ccatgatgat cgtcgggaac cgggtggacg
     2461 aggcccccga ccccctcgtg gagggacttc gggcccgggg gatccggttc gcgaccgccg
     2521 ccggggagct cgccctccgg aacgcggcct cggcagggat cgtgaagtac ctgcccgggg
     2581 atccctcttt ctccatcctc aagcctgaga tcctgaaccc tccccagagg gcagggctcg
     2641 agaggatcgc gggggtgatg aagaagttcg gcgggaccgg agtgcaggag gccctgaacc
     2701 acgccgtctt ctccctcctc gatcttatcg tggtgtaccc ggttgaggac gaacacaagt
     2761 atgccgacgg aaaggggcgg gtcctccccg atgcattcct gctgaagaag ggctcgacct
     2821 ctagggacct cgccttccgg gtgcacacgg agatcgggga gggattcctg tatgcggtag
     2881 atgcccggac aaagatgcgg gtgaaggaca cccacccgct gaaggatcgg gacgtcatca
     2941 aaattgtttc cacacgcaaa taaagacggg atgtcatcaa gatcgtctca ccccggaaat
     3001 gaagggagaa ttctgactgt ccgggacacc agcaactatt taacgcggac cttcgaacct
     3061 ccggataagc atgggagccg agatctacca ggcccaggtc atccggaact tctttgagac
     3121 catcacgggc ccggatcgca atctgacgcg gatctacatg tgcgtgatca gcctcgcgaa
     3181 gctccgcatg gaggcccccg agaagcagaa ggagctcctc gaccagctgc gcaaatcccg
     3241 ggagaagaag gagctctcgg tggacatcct ggactatgtc tgcagcgcgg ccatcgagct
     3301 cgagatcacc acggtccaga ccgccttcgg ggtcacggag atcgcgaagg ccgtccagga
     3361 cttcgaaaag gtcagcttcg actccctctg attctccttc attttgcctg gttctgtccc
     3421 accggagcac cggccttttc accggttcag ggactttcgg tacgtggcaa ggagctggag
     3481 gacgtacttc tcctgctcct cgagggcctg ctcgtcatcc ggtgaggagg cgagagcggc
     3541 tgcgtagatg atgatgagcg cgttcgtcag ctggaagaga tccgcgtcca cgaccggctc
     3601 cgaggggtcg atgatcccga agggcaggtc ggcctttgtg aagagaaagg ggagccgggc
     3661 gtcgtactgc gcctgggtga gggcctcggc ccgttcgcgg acgatccgga gggcgacccc
     3721 gagaaccgtc ggaacaggga tacctccgat gagcttcagg gcctgttcac accgggtgac
     3781 ggcatcgtgg tagatgccga gcttgaagga gaggaagacg aaggcgagct ggtgtccggc
     3841 agcctccggg attccggaga agtccggtct gacccgttcg atgtattcgg tgagatacct
     3901 gtccatgacg attaccccta tccttcgatt acggcgggaa cctccgctcg agcctgatca
     3961 ggaggcccgt gagggtcgcg gcgaagagga gctgcatggc gagccccgga ggtgtttcca
     4021 gggggaagac gaagatgagc agggcaaaga tgggggtgca gagggaaacg atgtcttttt
     4081 tcgtcctcgt gagggcgagc ccggcgatgg cgagggaccc gaggaagcac cccgccgaac
     4141 ccggaaggag ggaggggggc gaaaggagga acccggcgac gatcccgccc caggcgaggc
     4201 cagggacgag catccaggga tgcagccggc ctttgatgaa tgcggggaat gacccttctg
     4261 ccatcacgta ttctttactc tccccgataa acaagatttt ggtttggaga ggagagggtg
     4321 tctgacgtga cccttggaga agggatgaag ctctgttccc tctgtccgcg ggttatccgg
     4381 agcgacccgc ccttccctcc gcttcagccg aatccggttc cctggtccgg aggggccttc
     4441 ccgagagggc ctcggctctc cggctcacct ccgcgagaga ggcctgcatc ctcttgtcga
     4501 gagtcgagac ctcctccctc gcccgatcca gcccctcccc cgcccgcgcg agacgcttct
     4561 ccagttcctc cgcctccttc atgagctctg tccgcttcac cgaaaccggc ctcgcccgaa
     4621 gggcaacctg cgcggagtcc atctcctcag agatctcccg ataacgacgg gtgatcgagg
     4681 tgatccggtg aaccagatcc ccgggtctct cgatgaggtc gatctcctcc cggttcttcg
     4741 ggacgagctc ccccgaggcg gccatcgcgg ccagggcctt ctgtcccgcg ggcaggatcg
     4801 cgagagctgc ctccccgccc ggggggaggc ctccctcgag caggtccccc gtttcacgaa
     4861 ttgccttcag ggcctcgcgg tccttgcgtc ttgatgcgat cttctcccct ttccggagga
     4921 gatggatcgc ggtggccgac gcctcccgga aacggcgttc cagatcggcg tgctctccct
     4981 ccaggccccg gatcctgagg agctccttct cgaattcccg gtggtcctcc cccttctcga
     5041 gttcaccccg cgcccggacc gcggcctcca gggatcgacg ggaatccgcc acctcctcct
     5101 cccgttcccg gatcccgttc ctggccgcgg cgaggcgttc cctctctccg ccgtggagct
     5161 cgagggccgc ccggagcccg gcaagctccc tgaggcgttc ccgggcgcgg ccgacctccg
     5221 gggtcaggga gttcacctcc ctcccgatat cgtccacccc gtccctgagg accttcatct
     5281 cggcgggaaa ccgggaggcg agatacctcc cctggccccg gaaggccgag aggcagccct
     5341 tcaggatctc cccggccgcg gtgtagaacc cctccgggtc gtcggggagg tcccgggaga
     5401 ggctcatcct catggccctg aggaagaggg gaagggaccg ttccgtcacc cctgcgagct
     5461 tcaggctcga gacctcctcc atggaggccg cgtcgaagcc cgagagaacc ccctccatcc
     5521 ggccccgtgc cttcagcacg gcggggcggt ggcccgcgac cctagcggag aggtcggccc
     5581 ggatccgcga ctcctcggca tctaaccatg gcgggagatc gtcgataccg aggggagcgg
     5641 gggcctcatc agccttcctc cgccccttca ggaacctgaa catggacggc accggaacgg
     5701 gatcagtcgc tccgcgcgat ctgcctgagg cgctcgaccc cgtcgatctc ccggacgacc
     5761 tccacgacgg cgcggggaac caggtgctcc catggctcgc ccgcgaggat ccgggagcgg
     5821 atggcggtgc ccgagtgggt ctgcctctcg tacatgtccg ggcagtggac ggtgctcccc
     5881 gactcggcaa agagccgcat gacgagggga ttgttggaaa agacgcagtc gaagggtggg
     5941 gtcatggaac ggacatggga gacccagagg gcgttccgct ggatgtcctc gatggggatg
     6001 acgtacaggg gccgatcgag ggaggcgagg gagcgggtga tcatgaggac ccgttccccc
     6061 gcggtgaagg gattctcgag ctcgtgggag agctgcgcgc tccccacgcc gatgacgatc
     6121 tcgtccacct cacccgccac ccgctcgagc accgcctggt ggccgttgtg gtacggctgg
     6181 aacctcccga tgtagaaccc ccgggtcata gccccgccac catggccgcg atccgggcgg
     6241 cgaaggcccc cgcggtgaaa cccgcatcga tgttcacgac ggcaaggacc gagcaggact
     6301 ggagcatgga ggcgagggcc gccctcccct cccccatgta cccgtatccc gaggagacgg
     6361 gaaggccgac gaccggccgg tccacgagcc ctgccaccac cgcggggagg gtcccctccc
     6421 ggcccgcggc gacgatgaag acatgggcgt cgaggaccga tttgagggca gggaagagcc
     6481 ggtggatccc cgcagccccg acgtcgtacg cggtccggac ctcgcagccc atctcctcgg
     6541 cgatgagctt tgcctcttcg gcggcgggga gatcagcggt gcccgcggtg aggatggcca
     6601 cgatacctcc ggtcttttcc tggaccggta accggaaggc gacgatggcc cggggaactt
     6661 cgaggtgctc cgcctggaac ccctcctccc cgcagcgcct ccggatctcc tctgcctggt
     6721 cgggggtcac cctcgtcacg atgcaccgcc cctgggccgc gaccgcccgg accgtgatct
     6781 cggcgagctg ggcgggcgcc tttccctcgg cgaggatgac ctcggggacc ccgcaccggg
     6841 cggccctacc caggtcgaga cgggcatact ccccgagctg ctcgatccgg agcccctcga
     6901 tccgggaggc cgcctcctcc cgggtgagct ccccgcgccg gtaggcctcg aggagcccga
     6961 ggagatttgc atggggctgc atggaactga atagagatat gggcgcaggt atcctaatta
     7021 gtatttgcca tgagttacct catctttgtc gccggattcg gcgccctcgc cacctatctc
     7081 ctctggttcc gggacgtgcg gatctttttc cggaccggcc ttcccggcta ccgggcggcg
     7141 gcccgccacg gggttgccta cgccaccctc gccctcatcg gcgtggacgt gggcctcatc
     7201 ttcgacgagg tggtcggcct cggcctcatc ctcctcgccc tctacctcca gggcagggaa
     7261 aagcgggaga aggtcttctc cggcggggag cccgccctcg accggctcct ggggaaaacg
     7321 gcccaaagga aagataagga gagagaatag aattaccggg tgaagtggca tttcgtgaga
     7381 aatgacactt ggtcgccgaa ggtgactcga gaagatgctc cagagaccga ggggaaccag
     7441 ggattttctg ccggaggaaa tggagagaag gcgttcggtg gaggtatgcc tccgggagag
     7501 ggtggcacga tgggggtacc gcgaggtctg caccccggag ttcgaggagc tcgagctctt
     7561 caccctcagg tcgggagcgg ggatcgtcgg tgagatctat gccttcgagg acaagggggg
     7621 caggaagatg gccctccggc cggagatcac cgccccggtc ctccgcatgt tcgtctccga
     7681 ggcgaaggtc cacccgaggc ccctcaggtg gttctacttt gccgactgct tccggtacga
     7741 gcggccccag aaggggcggt accggcagtt ctggcagttc gggaccgagc tcatcggggc
     7801 ggacaccccg agggcggatg ccgagacgat cctcctcgca gacgaccttc tcagggccgc
     7861 ggggatcccc tgcgacctcc acgtcgggca cctggccccg atgaagcacc tcctcgcgga
     7921 tctcgacccg gcggcccagg caaaggccat ggccttcctg gacaagcggg acttcgacgg
     7981 gctcacctca cttctcgccg gttccgggcg ggatgatctc gtggataccc tgagagccct
     8041 cgcccacgcg cgaaccctcc ccgagctctt cgggatcacg ggggagattc ccgagcggag
     8101 gcggatcgag gagaccgtgg agatcctcga gaagaaccat ctcgtgtaca cccttgattt
     8161 cggaatcgcg aggggactgg actactacac cggtgtggtc ttcgaggggt ttgccccgaa
     8221 cctcggtgcc gagaaccagg tgctcggggg cggtgcctac cggctcgccc acctcttcgg
     8281 cggggacgac gtcccctcct gcggctttgc catcggcttc gaccggctca tggtctccct
     8341 cggcgagatg gagatgcccc ggcccgtcag ggtggcggtg tgctcgaccg aggagggacg
     8401 gatccgggcc ttcgaagtgg caaaggcctt cagggacggg ggggtttccg ccgagatcga
     8461 cctcctcgac cggaatttgg gggcccagat cgcccacgcg gcgagatccg cggattacgt
     8521 ggcgatcctc gggaagcggg aggtggagac cgggacggtg accctgaaag acctccagac
     8581 cggcgagcag aaggccgtcc cgctccccga cgccgtggcc gaggtggccg cccgtgtccc
     8641 tcgctgagga gctcgcccgg aagcagcggg ccatcagcat cgccgagttc ttcgagaaga
     8701 acaagcacct gctgggcttc gactccccca cgagggggat catcaccacc gtgaaggagg
     8761 cggtggataa tgccctcgac gcatgcgagg aggcccaggc cctcccggac atcctcgtcc
     8821 aggtgaagaa gatctccgac gaggtcttcc gggtgacggt cgaggacaac ggcccgggca
     8881 tcgtccccgc ccaggtcccc ttcgtcttcg ggaagctcct ctacggctcc cggttccacc
     8941 agatcaagca gagccggggg cagcagggga tcgggatctc ggccgccgtc ctctacgccc
     9001 agctcaccac cggggttccc tccctcgtta tctcccgcac ggatgcaaag aaacccgcct
     9061 cccggttcga gctcgcgata aagatcgagt ccaacgaacc cgaggtggtc tcgcaggagg
     9121 aggtggagtg ggaccggacc catgggaccc gggtggagat cgagctccgg gcgaccctcg
     9181 ccgcaaagaa gcgcctcatc gactacctga agtacacctc ggtggtgaac ccgcatgccc
     9241 ggatccgggc cgagatcgac ggggaggtct acaccttcga ccgggtgagc catgatccca
     9301 tccccctccc ctgccccatc cagccgcacc cccatggcat cgagatcggg ctcctgaagc
     9361 ggatggccgc cgagagcccc ctccccctgg aaggattcct cgtcgagtcc ttcagccggg
     9421 tggggaagaa ggcggcggag gagatcctga ggaaggcggg cctgtccggc gggaagaagg
     9481 cgaagtccct cacgaatgag gagcaaaagt ccctccttgc ggcgatgcag gcggtcgcca
     9541 tccccgcccc cccgaccacc cagtgcctct cgcccatcgg cgaggagctc atcaggaaga
     9601 gcctggacaa ggagttccag ctcgacttcg tgaaggccaa gacccggccg ccgtcggtgt
     9661 acagcggcca ccccttcgtc gtggaggcgg ccatcggcta cggcgggagg cttgcctcgg
     9721 aggagaaggc catcatcctc cggttcgcaa accgcgtccc cctcatgtac cagcagggtg
     9781 cctgtgccat cacccaggcc atcgccgggg tgaactggcg gaactatggc atctcccagg
     9841 acggactccc cgtcgggccg gccctcatcc tcgtgcacgt cgcctcgacc aatgtcccct
     9901 tcacgagcga gtccaaggac gcggttgccg ccatccccga gatcgagaag gagatcgacc
     9961 tcgcgttaaa ggatctgggg agggagctga agaccttcct ctcccggcgg gaccggcacc
    10021 gggtccagga ggaccgtgcc cgggccctct gcgccatcat ccccgagctc gcggaaaagg
    10081 tcgccgaggt gaccgggaaa ccggtcccgg acatcacccc catcgagggg cgaatcatgc
    10141 gccggctcgt ggcaaagaaa tggacctccg acggacaggt gcgaatcgcc gtccggaact
    10201 acaccgacaa ggagatctcg ccgaccgtgt acgacacatc cccggacccc gcacccgatg
    10261 ccgagccgaa gcccggctac acccatgccg tcgagaagga tttcacgagg gtctggaagc
    10321 tcaccctcgc ccccggcgag gggtgggagg cgaagtacac aggggccggc gggggaactc
    10381 tcgacatcca gggggtcgag gaggccgtga agctggtggt ggacctcgat gtctaaggag
    10441 gagttcgaca gcctggcgag gacgcgcctc ctcgccatcg ccctcgagtg gtataacgag
    10501 atgcagggcg gggagatccc gtatatccgg ctcccgacga ggacgaaggc gaacatcgag
    10561 tacgacagcg ggagcgaggt ctggaagtac ggcgaccggg agagcctccg ggccgcgact
    10621 tcggagaagg gggccctcca cctcctgaag atggcgtacg tcatcggctt catcaagcag
    10681 cagctcgccg agaaccggtc ctcgaccctg agggagctct actacatctc cgagggatgg
    10741 aagcgggcga agttcggggc tcaggacgag agtaacctcc tcatcgagga tctcgagatc
    10801 atcaccgacc tccagcggga ggccttccac cttcgtcccg aggaggatgg cgcgacggtc
    10861 ttcgggcccc tcaggatccg ggagaagacc cgtcgcgggg tgcgggacat ccactgcagg
    10921 gacgacgtcg gggaggcagg ctaccagatc ccgaacgacg tcgagaccgt ggagttcctg
    10981 gagcatgacg cgaagttcgt gatagccctc gaaacgggcg gcatgtacgc ccggctcatc
    11041 gagaacggct tcgacgagga gcaccaggcc atcgtcgtgc acctgaaggg ccagccggca
    11101 cgctcgaccc gccggatcct tcaccggctg aacgagcagt tcggactgcc catcacggtc
    11161 ttcaccgatg gcgacccctg gtcgtaccgg atctttgcct cggtggccta cggggccatc
    11221 aagagcgccc acatctccga gctcctggcg acgccgaagg cgcagttcat cggcgtgcag
    11281 cccacggaca tccgggacta caacctccct tcggacggtc tcacggacaa ggacgtagac
    11341 gcgctccggg ccgagctctc ggacccccgg ttcgacagcg agtactggaa aggccagatc
    11401 aacctccagc tcgagatgaa gctcaaatcg gaacagcagg cctttgcggc ccgggggctg
    11461 ggtttcgtga caaaggaata cctgccctcc cgtctctcgg agatgggggt tatctgaccc
    11521 gcccgtcccc ctttaccgga ccccggtctc cttatccgac ccggccttcc tgcctaccgg
    11581 accctcccac gctcaaacgc gagcatgtcc ttcagggact gggtgaggga gatcaccggc
    11641 ctccacccgc aggtcgcggt gatgagggaa ttgtcgctcc tctgggacgg gatggggacg
    11701 ggcatctcct tttccgggag gagcacgggg tggcgtttcc ccgtgatcct cctcaccatc
    11761 tcgagaacct cggcgaccga ggagtccttc ccgctcccga cgttgaaggc cttcccggtg
    11821 cagcgctccg gaaactcggg gagggtgacg agatcccagc acgcgacggc caggtccctc
    11881 acgtcgacga agtccctcct cgagctgaca ccggagaggg agatctcctc cctcttcccc
    11941 tcgtcgatct cggtcacctg cctcacgatc ttcccgcaga cgtaggcatc cgactgcccg
    12001 ggcccgacca ggttgaaggg ccggatgacc gcgacgggga ggtgacggac ggccccgtac
    12061 gactgggcga ggaggtcggc cgctgccttg gagaccccgt agggcccgac agggcgaagg
    12121 agggccgatt cggggatcgg accgctcccc gcgtacccgt actccgccga ggatccggtg
    12181 acgagaacac gcacgggagc cccgagggtg caggccgcgt cgaggatgtt ctgggtggcg
    12241 atgacattgg attccaggag ctccgccatg gtcccgtggt tcagccccgc gagatggatg
    12301 attgcgccgg ggccggcctc cttcacccga gagagggtct tctcacggtc ggcgagatcc
    12361 agcttcacgg gggtgaacct gtcggaggga ggggggaggt tcctccggta gacgccgagg
    12421 acctcgtggt cgcccttggt accgagaatc tgccggacca tggcgcggcc ggcgagcccg
    12481 ccagcgcccg tgacgaggac cctcatccca ggaagagatc ccggtagcgc tcgaactcga
    12541 cgatggagcg ctcgtagtcg tcaggccgtc cgatgtcgag ccagtacccc ggatggggat
    12601 acgagtagac cggggcatct ccggcgatca tcgcgagcat gagctcgtcg aagccgaagg
    12661 gccttccctc ggggacaaaa ccgaggatct cccgttggaa ggcgtagatc cccatggaga
    12721 cctcgaaggg ataggtgggc ttctcccgga aggcgcgaat cctcccctcg gagtcgggct
    12781 cgaggacccc gaagtccacg ttcacctccc ggtg
//
LOCUS       DATGDI010000047        11958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_382601, whole genome shotgun sequence.
ACCESSION   DATGDI010000047 DATGDI010000000
VERSION     DATGDI010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 11958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11958
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382601"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(412..1149)
                     /locus_tag="VKO45_01810"
     CDS             complement(412..1149)
                     /locus_tag="VKO45_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMA04640.1"
                     /translation="MPEISIVLVGPLHEGNVGFSARVMKNFGFTDLVLVDPCPLGDLA
                     KACAMHASDVLENARTMTLEDVFSGYPLIIATTGGLSKSVCRSMRMPYFDPSEIRDQI
                     GKALGKVAILFGRENRGLSNEEIRRCDLICTIPSSSAYPILNISHAVGIVCYELAGLP
                     RGAYRLAEREEVECLYDHLDRFLDRIEHPGFKRKNTLLMARRILGRTLLTTREVSTIH
                     GILRRTEWHMKEEPEKPETENEKHNEE"
     gene            complement(1168..1854)
                     /locus_tag="VKO45_01815"
     CDS             complement(1168..1854)
                     /locus_tag="VKO45_01815"
                     /EC_number="2.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerol geranylgeranyltransferase"
                     /protein_id="HMA04641.1"
                     /translation="MQPPWRSWVHVTKLDPDRPLAPGLVEGVVTSGTDAIILSGTLNV
                     TQENLARLLSAVKDHGLPLVVEPAGPEAVRFEGIDLLFVPSVLNSRDTQWIVGKHQTW
                     VMKQQVQWEKVVPEAYLVLNPDSAVGKVTRADCGIGPREVAAYATCADRYFRFPIVYI
                     EYSGIYGDPAVVKAASEALHDAILYYGGGIDSGKRAAEMSRYADTIVVGNAVYEKGIG
                     VLKETVDAVQ"
     gene            complement(1887..4700)
                     /locus_tag="VKO45_01820"
     CDS             complement(1887..4700)
                     /locus_tag="VKO45_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HMA04642.1"
                     /translation="MNTLVVAEKNISAERIARILAGKDRVTVGKETGISTYSFDDMVV
                     VGLKGHVVEVDFAPGYDDWRSEENPPRSLIDAGTRKVPTEKRIIATLQKLARKAGEVI
                     IATDFDTEGELIGKEAVDLIREVNPKVPVRRARFSAITPQEIQGAFSDLKDLDLALAS
                     AGEARQVIDLVWGASLTRFISIAARRGGKNILSVGRVQSPTLAMIVDREKEIAAFVPE
                     KYWTLQLTSEKEGEAFTARHATARFTGKEEAEGARDRTRPPLLVREVKEGEKVDRAPA
                     PFDTTGFLVAAGRLGFSASNAMRIAEDLYMNGFISYPRTDNTVYPRSLSLESILAILQ
                     GSSFAREVAWVRENRRKEPTRGKKVSTDHPPIHPTGSATREILGEDRWRVYELVVRRF
                     LATLSPDAVWKTVRCGLEAGGEPYVANGARLAVPGWRKVYIYQEAEDHVLPPLQVGER
                     LPILEILLEEKETQPPPRYTQSRLIQRMEELGLGTKSTRHEVIAKLLGRRYAEGNPLR
                     PTPVGMAVIESLEDHADAITKPEMTRTLEADMQEIKVRNRTPDFVVGESREMLHRVFN
                     ALEEHGEEIGREIMERADEERVIGPCPACGADLRIRAGGQSQFVGCSRYPECGFNINL
                     PSAQWGKAITTSTCCPEHGLRHVRLIRKGARPWDIGCPLCSHIRASEESMALIPSMTK
                     ETMKRLRDHHIYTVSELAGQEPGNLARILGISPEETRHLLAGAGDALALLRRRSELRR
                     FLRVHLPPRKGRSRAEIMKKLGETGVDDLPSLSRSDATTLAKAGIGPKESAVLLAEAR
                     SAIDSRVLRDAGIPPASLKRYHEAGISRPEDLCFLHPVFLADLTGLSLDTVYRHAGLV
                     CEAMGAKVPVKVPKARVEKGRQELLAVPGLGEATLEKLHRAGILDRDDLVTADPDDLA
                     KKTGIDPGVIRGFAGHFARKGKR"
     gene            complement(4697..6127)
                     /gene="gatB"
                     /locus_tag="VKO45_01825"
     CDS             complement(4697..6127)
                     /gene="gatB"
                     /locus_tag="VKO45_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HMA04643.1"
                     /translation="MTVIIGLEIHCQLTTQSKLFCGCSTDYRDDPPNTHTCPICLGLP
                     GSLPSLNRRAVEYALRVAKALNCTVPDEAEFARKNYFYPDLPKGFQITMYDRPLAVEG
                     FLDIEADDGTEKRIRITRVHMEEDPGRLVHVGSGDRPRYSLVDYNRSGIPLIEIVTEP
                     DLRSPKEARRFINTLRTTLEYLGVFDGDREGGLRVDANISMEGGNRVEVKNISSYKGV
                     EKALTFEITRQRNVLRRGLAVERETRHFLEARGVTTSARSKEEEHDYRYFPEPDLRPL
                     RVREWCEEIVLPELPAARRARFVAAYGVSPSHGRTLCGDLRLADFFEQVACCGDPVLA
                     ATWVADTLLGELYYRDMAIGRVPPGHFVELLGLLRGKEITEKAGVEVLRVILDQVSRG
                     ETPETPTAIVARLNLGKTEGDAFGPVIREVVESNPSAVRDFRSGKGEALNFLVGQAMK
                     RTRGKADPKELARMIREAIANPGVAG"
     gene            complement(6124..7377)
                     /gene="gatA"
                     /locus_tag="VKO45_01830"
     CDS             complement(6124..7377)
                     /gene="gatA"
                     /locus_tag="VKO45_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HMA04644.1"
                     /translation="MTRYDGEPGREGRLAGIRIGVKDNISTGGIPTSCGSRILEGYTP
                     PYNANVVDLILREGAGIAGKTNMDEFGMGSSTENSAFGPTRNPLDPDRVPGGSSGGSA
                     AAVAAGMVPMALGSDTGGSIRCPAAFCGIVGLKPSYGRVSRYGLVAYANSLEQIGPLA
                     KTVKEVSLLMEVISRYDPRDSTMLDRPFSPRFTGDVKGLRIGVPSEFFGKGVDPEVAR
                     KVREAIADLEEMGARAIPCSMPSMAYALAAYYVTCTSEASSNLARFDGVRYGPPDDTR
                     KSWHDAFREVREKGFGEEVRRRILLGTFALSAGYYGKYYGKAQAARRNVKADFSRIFR
                     DVDVIAGPTMPTVAWRIGEKTDPLSMYLSDILTVPANLAGIPAVSVPCGTAHGLPVGL
                     QFMGKPFEDERVLEVAYAYEQGVRR"
     gene            complement(7422..7694)
                     /gene="gatC"
                     /locus_tag="VKO45_01835"
     CDS             complement(7422..7694)
                     /gene="gatC"
                     /locus_tag="VKO45_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HMA04645.1"
                     /translation="MISPDDVEKIAGLADIGLARERLSGFTREFNAILEYFDILDTVR
                     EEARAAGGEVNVMREDEITPCLPQGEVLSLSSEPEEGFIKAPRVME"
     gene            complement(7681..8697)
                     /locus_tag="VKO45_01840"
     CDS             complement(7681..8697)
                     /locus_tag="VKO45_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HMA04646.1"
                     /translation="MTGSPRIRLQGWVELGGRKLTLPEVEALVGADPGRVAGFGGEFY
                     LTWDGCTASDRLGILGIPGPFGIVTCGGNAVGEVNPDSPTLGLEEALRTAVRLRADQG
                     VVSLSGGVDSSLIACLAGLPCVTVGMEGSRDLEKGGAAAQALGLSWEGVTITEDELEA
                     ALPRVTGAIPGPAPLDVAIATGFFFVARWAAGRGYRRILVGQGADELFAGYARYRTAA
                     DIPGMLARDFASLRGQALRDQAVARLHGTSFSLPYLDLRVVRAAQALPPGELLRGGTG
                     KIPLRELAARLLTPDFARAPKKAMQYGSGIAGAIRGLAGKNGYKRSVREYLIQLLGEG
                     HDLT"
     gene            complement(8694..10073)
                     /gene="purB"
                     /locus_tag="VKO45_01845"
     CDS             complement(8694..10073)
                     /gene="purB"
                     /locus_tag="VKO45_01845"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HMA04647.1"
                     /translation="MAIHPIESRYGTPEMRAVWTEEHRFTCILRAEAALAQAEARCGL
                     IPEKDAKAIARGAQKASLGRAKEIEAEINHDMMAVVKALSEVSGDAGRWVHFGATSND
                     ILDTATGLQLKESLALLETKLLSLLSVLLRRAGETRTLVCAARTHGQIGVPTTYGMRF
                     ALWASEIARHVDRLREMRPRVEVGKMAGAVGTMAALGKDGMAVQEKMMGELGLGIPDI
                     TNQIVPRDRYAEYIMFLAGFATTLDRMAITVRTLQRTEIAELEEPFGKKQVGSSTMPH
                     KRNPIRSEQVSGLARVIRSAVEPALQDNTLWDERDLTNSSCERVILPEATILADHILQ
                     VMTTVMDGLTINAGNVKKNLTILDGIPMAESVMVELTKKGMGRQDAHEIVREASMEAY
                     RKGHDLATVLAGNPAVKKLISRKALAGLLDPAKYIGTAPVQVERVIARLSKYLDRKAP
                     APKTPSRRR"
     gene            10130..>11958
                     /locus_tag="VKO45_01850"
     CDS             10130..>11958
                     /locus_tag="VKO45_01850"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HMA04648.1"
                     /translation="MDAISAFFPYEAYRPHQRQMLELVAQTAEKGGIAMIDAPTGSGK
                     SSVVSALLAGRRDRTVVIAVRTVSQLATFVRELTLVRKRRPELRFAYLIGKGQMCRLG
                     GGGNVYRRCEGLKSLSTALMRDRAEAGSLVPARDRLVRQQIRKTTVDHPLICPPFIHS
                     RSFASEEGAGLRMVPSAALRTRAERVGRETIWPEELPAASGDCCPYEVMLHAARGADV
                     ILLNYHHVFDESIREQLYLQLGIGAAEVLLLVDEAHNLGDAVQSVQSVSVGYRDLEGA
                     ESELQRLRGKVRAVEAVGRVFPQVRRFMELLRDSREGEDWFDPAIFERMVVKGSLYAD
                     MEAIVDDMLSVSDAMKERNLKAGDFRETSVERLTTFLYRIFLSAHDPAYLTVYRREEE
                     EVILEVRNIDPQAKILELAQSHAATVLISGTLSPAESFRRLYFGDEPAAVLTLPNAFP
                     TENRRIVVARDVTTLYSMRDKAGNNDRIAGYITEFSRLPGNLAVYFPSYKILSSFADK
                     CAGSIRGKEVIVEPRDPKDASAALRSFLTLPEQGRSGVIFAVCGGKWSEGLDYRGELL
                     TGAMVVGLPLAPYTRVRAMIIDYFRRKFGEEGEFLSYTLPAMNR"
BASE COUNT         1879 a         3994 c         3936 g         2149 t
ORIGIN      
        1 aggttcacac aacggggaag gggtcacaac aattttcata ttcattttcg tggtgtgtct
       61 cggggggctc acccgccccc cgccgcacgg gcatcccccc cgtgtacgga ggcttatgca
      121 tgaggtttcc gatatttccc gaaggatcct ccctatgcga gacaggccta tcctcacgcg
      181 ggggcagccc cagcggcgag ggcatacgag gggatgcata tccccgagta tggagcaccc
      241 gtcagggtgc gatgagccga tggcgagtcc acgactgcgt cgctcaaggt cggtttctgc
      301 gactgcgtcg ctcgaggtcg gtttctaacg aaacctccct cttacgaaac ctcccttccc
      361 ccaggagcgt ccaggacgtc ctatgcaaac tcaaggatga tgagagaccg attactcctc
      421 gttatgcttt tcgttttccg tttccggttt ttccggctcc tccttcatat gccattccgt
      481 cctccggagg atcccatgga tcgtcgagac ctcccgggtg gtgagtaagg tccggcccag
      541 gattctccgc gccatgagga gggtgttttt ccgcttgaag ccgggatgtt cgatccggtc
      601 gaggaaccga tccaggtgat catacaggca ctccacctcc tcccgttccg cgaggcggta
      661 cgccccccgg ggaagaccgg cgagctcgta gcagacaatc cccacggcgt gggagatgtt
      721 caggatgggg taggcggacg aggatggaat ggtgcagata aggtcgcacc tccggatctc
      781 ctcgttggat agtccccggt tctccctccc aaagaggatg gcgaccttcc ccagggcctt
      841 tccgatctgg tcccggatct cggacgggtc gaagtaaggc atccgcatgg acctgcagac
      901 ggacttcgag agcccgccgg tggtggcgat gatgagcggg tatcccgaga agacatcctc
      961 cagggtcatg gtccgggcgt tctcgaggac atcggaggcg tgcatcgcgc aggccttcgc
     1021 gagatccccg agggggcagg ggtccacgag aacgaggtcg gtgaacccga agttcttcat
     1081 gacccgggcg gaaaagccga cgtttccctc gtggagaggt ccgacaagga cgatggatat
     1141 ctcgggcatc cgtgcgccgc ccgcggccta ttgcacggcg tcgacggtct ctttcaggac
     1201 gccgattccc ttctcgtaca ccgcgttccc cacgacgata gtgtcggcgt acctcgacat
     1261 ctcggccgca cgtttcccgg agtcgatacc cccaccgtag tagaggatgg catcgtgaag
     1321 tgcctccgag gcggccttca ccaccgcggg gtcaccgtag atgccgctgt actcgatgta
     1381 cacgatgggg aaccggaagt accggtcggc gcaggtggca taggcggcca cctccctggg
     1441 gccgatcccg cagtccgccc gggtcacctt cccgacggcg gagtcggggt tcaggacgag
     1501 atacgcctcg gggaccacct tctcccactg cacctgctgc ttcatcaccc aggtctggtg
     1561 cttcccgacg atccactgcg tgtcccggct gttcaggacc gagggaacga agagcaggtc
     1621 gatgccctcg aaccgaaccg cctcgggacc cgcaggctca acgacgaggg ggaggccgtg
     1681 gtccttcacc gctgagagga gccgggccag gttctcctgg gtcacgttca gggtgcccga
     1741 gaggatgatg gcatcggtgc cgctcgtcac gaccccttcg acgaggccgg gggcgagggg
     1801 ccggtcgggg tcgagcttgg tcacgtggac ccaggatctc caggggggct gcatggtctc
     1861 ccacagtcct tttcgcatgg tgaggattat ctctttccct ttctcgcgaa atggcccgcg
     1921 aacccgcgga tgaccccggg gtcgatcccc gtcttcttcg cgagatcgtc cggatcagcg
     1981 gtgaccagat cgtcgcggtc gaggataccg gcccggtgga gcttctccag ggtcgcctcc
     2041 ccaaggcccg gcacggccag gagctcctgc ctccccttct ccacccgcgc cttcggaacc
     2101 ttcaccggga ccttcgcccc catggcctcg cagaccaggc ccgcgtgccg gtacacggtg
     2161 tccagggaga ggccggtcag gtcggcgagg aataccgggt ggaggaagca gaggtcctcg
     2221 ggccgcgaga tgccggcttc gtggtagcgc ttcaggctcg cgggggggat tcccgcgtcc
     2281 ctgagcaccc gggagtcgat ggcagatcgg gcctcggcga ggaggacggc cgattccttc
     2341 ggcccgatgc cggcctttgc gagggtcgtg gcatccgatc tcgagaggga cgggaggtca
     2401 tcaacccccg tctcccccag ttttttcatg atctctgcgc ggctccgtcc cttccgcggg
     2461 gggagatgga cgcggagaaa cctgcggagc tcggatctcc ggcggaggag ggcgagggcg
     2521 tcccctgccc cggcgaggag gtgcctcgtc tcctcggggg aaatccccag gatccgggcg
     2581 aggttcccgg gctcctggcc ggccagctcg gagacggtgt agatgtggtg gtcccggagc
     2641 cgtttcatgg tctctttcgt catggagggg atgagagcca tggactcctc gcttgccctg
     2701 atgtgggagc agagggggca cccgatgtcc cagggccggg cgcccttccg gatgaggcgg
     2761 acgtgccgca ggccatgctc cgggcagcag gtggaggtgg tgatggcctt cccccactgg
     2821 gcagagggga ggttgatgtt gaatccgcac tcggggtacc ggctgcaccc gacgaactgg
     2881 ctctggcctc ccgcccggat ccggaggtcc gcaccgcagg ccgggcacgg cccgatgacc
     2941 cgttcctcgt cggccctctc catgatctcc ctcccgatct cctcgccgtg ctcctcaagg
     3001 gcgttgaaga cccggtgaag catctcccgg gactccccga ccacgaagtc cggggtccgg
     3061 ttccggacct tgatctcctg catatccgcc tcgagggtcc gggtcatctc cggcttcgtg
     3121 atggcatctg catggtcctc gagagactcg atgaccgcca tcccgacagg agtcggccgg
     3181 aggggattcc cctccgcgta gcgccggccc aggagtttcg cgatgacctc gtgcctcgtg
     3241 ctcttcgtcc cgaggccgag ctcctccatc cgctggatga gcctcgactg ggtgtagcgt
     3301 ggcgggggct gggtctcctt ctcctcgaga aggatttcga ggatgggaag cctctcgcct
     3361 acctggaggg gtggcaggac atggtcctcg gcttcctggt agatgtacac cttccgccag
     3421 ccgggaacgg cgagccgggc accgttcgcc acatagggct ctccgccggc ctcgagtccg
     3481 caccgcaccg tcttccagac ggcatcgggg gagagggtgg cgaggaaccg gcgcaccacg
     3541 agctcgtaga cccgccagcg gtcctcgccg aggatctccc gggtcgcgga cccggtcgga
     3601 tggatgggcg ggtggtcggt ggagaccttc ttcccccggg tgggttcctt ccgccggttc
     3661 tcccggaccc aggcaacctc cctcgcaaac gaggatccct ggaggatggc gaggatggac
     3721 tcgagggaga ggctcctcgg gtacaccgtg ttgtcggttc tcgggtagga gatgaacccg
     3781 ttcatgtaga gatcctcggc gatgcgcatg gcgttcgagg ccgaaaaccc gagcctcccc
     3841 gcggccacga ggaaccccgt ggtatcgaag ggggcggggg cacggtccac cttctccccc
     3901 tccttcacct cccggacgag gaggggcggc ctggttcggt cccgggctcc ctcggcctcc
     3961 tccttccccg tgaaccgggc cgtggcgtgc cgggcggtga aggcctcccc ctccttctcc
     4021 gaggtgagct ggagggtcca gtacttctcg gggacaaagg ccgcgatctc cttctcccgg
     4081 tccacgatca tggcgagggt gggggactgg accctcccca ccgacagaat attcttccct
     4141 ccccgccgcg cggcgatgga gatgaaccgg gtgagggagg ccccccagac gagatcgatg
     4201 acctgacggg cctcgccggc cgaggcgagg gcaaggtcga ggtcctttaa gtcggagaag
     4261 gctccctgga tctcctgggg ggtgatggcg gagaaccggg cccttctcac gggaactttc
     4321 gggttcacct cccggatcag gtccacggcc tccttcccga tgagttctcc ctcggtatcg
     4381 aagtcggtgg cgatgatgac ctcgcccgcc ttcctcgcga gtttctggag ggtggcgatg
     4441 atcctcttct cggtggggac cttccgggtg ccggcgtcga tgagggacct cggagggttc
     4501 tcctcgctcc gccagtcgtc gtagcccggc gcgaagtcca cctcgaccac gtgccccttc
     4561 aggcccacga ccaccatgtc gtcgaaggag taggtggaga tgccggtctc cttcccgact
     4621 gtcacccggt ccttccccgc gaggatccgg gcgatccttt cggccgagat gttcttctcg
     4681 gcgacaacga gggtgttcac ccggccaccc ccgggttcgc gatggcctcc cggatcatcc
     4741 gggcgagctc cttcgggtct gcctttcccc gggtccgttt catggcctgg ccgacgagga
     4801 agttcagggc ctcgcccttc ccgctccgga aatcccggac ggccgagggg ttggactcca
     4861 ccacctcgcg gataaccggc ccgaaggcgt ccccctcggt cttcccgagg ttcagccggg
     4921 ccacgatggc cgtcggggtt tcaggtgttt cccccctgga aacctggtcg aggataaccc
     4981 ggagcacctc caccccggcc ttctccgtga tctcctttcc ccggaggagg ccgaggagct
     5041 ccacgaaatg gccggggggg acgcggccga tggccatgtc ccggtagtag agttccccga
     5101 gaagggtgtc ggcgacccag gtggccgcga ggacgggatc cccgcagcag gccacctgct
     5161 cgaagaagtc agcaagcctc aggtccccgc agagagtgcg gccatgggac ggggagaccc
     5221 cgtacgccgc gacgaaccgg gcccgccgtg cggccgggag ctcggggagg acgatctcct
     5281 cgcaccactc cctgacccgg aggggccgga ggtcgggctc ggggaagtag cggtagtcat
     5341 gctcctcctc cttggaccgc gccgaggtgg tgaccccccg ggcctccagg aagtgccggg
     5401 tctcgcgctc gacggcaaga ccccgccgga gaacgttcct ctgcctcgtg atctcgaagg
     5461 tgagggcctt ctccaccccc ttgtacgagg agatgttctt cacctccacc cggtttcccc
     5521 cctccatgga gatgttcgcg tccacccgca ggcctccctc ccggtcgccg tcgaaaacac
     5581 cgagatactc cagggtcgtg cggagcgtgt tgatgaaccg cctcgcctct ttcggagacc
     5641 ggaggtcagg ctcggtcacg atctcgatga gagggatgcc gctccggttg tagtctacga
     5701 gagagtaccg gggccggtcc ccgctcccga catggacgag ccggcccggg tcctcctcca
     5761 tgtggaccct cgtgatccgg atcctcttct cggtcccgtc gtcggcctcg atgtcgagga
     5821 acccctccac ggccagggga cggtcataca tggtgatctg gaaccccttc gggaggtcgg
     5881 ggtagaagta gttcttcctt gcgaactcgg cctcatcggg gacggtgcag ttgagggcct
     5941 ttgccacccg gagcgcgtac tccaccgccc tccggttgag ggacgggagg gagccgggaa
     6001 ggcccaggca gatggggcag gtgtgggtgt tcggggggtc atcccgataa tcggtcgagc
     6061 agccgcagaa gagcttgctc tgtgtcgtga gctggcagtg gatctcgagc ccgatgatga
     6121 ccgtcatcgc ctgaccccct gctcgtacgc gtaagcgacc tcaaggaccc gctcgtcctc
     6181 gaagggcttc cccatgaact ggagcccgac ggggagaccg tgggccgtgc cgcacgggac
     6241 ggataccgcg gggatgccgg ccaggttcgc gggcacggtg aggatgtcgg agaggtacat
     6301 ggagagggga tcggtcttct ccccaattcg ccaggccacc gtgggcatcg tcgggccggc
     6361 gatcacgtcc acgtccctga agatccggga gaagtctgcc tttacgttcc tccgtgcggc
     6421 ctgggccttc ccgtagtact tcccgtagta ccccgccgag agggcgaagg tcccgaggag
     6481 gatcctccgg cgaacctcct ccccaaaccc cttttcccga acctctcgga aggcatcatg
     6541 ccaggacttc cgggtatcgt ccggcgggcc gtaccggacc ccgtcgaaac gggcgaggtt
     6601 cgaggaggcc tcgctcgtgc aggtgacata gtatgcggcg agggcatagg ccatggaggg
     6661 catcgagcag gggatcgccc ttgcccccat ctcctcgagg tccgcgatgg cctcccgcac
     6721 cttccttgct acctcgggat ccacgccctt cccgaagaac tcggaaggaa ccccgatacg
     6781 gaggcccttc acgtcgccgg tgaacctggg ggagaagggc cggtcgagca tggtgctgtc
     6841 gcggggatcg tacctgctga tcacctccat gaggagggaa acctctttta ccgttttcgc
     6901 gaggggcccg atctgttcca gggagttcgc gtaggcgaca aggccgtacc gggagaccct
     6961 gccatagctc ggcttcagcc ccacgatccc gcagaaggcc gcggggcacc ggatggagcc
     7021 acctgtgtcg gacccgaggg ccatggggac catgcccgcc gcgaccgccg cggcggagcc
     7081 cccggatgac ccgccgggga cccggtcggg gtccagggga ttcctcgtgg gcccgaaggc
     7141 gctgttctcc gtcgaggaac ccataccgaa ctcgtccatg ttcgtcttcc ccgcgattcc
     7201 ggctccttcc cgcaggatga ggtcgaccac attggcgttg taaggggggg tgtaaccctc
     7261 gaggatccgc gagccgcagc tcgtggggat ccccccggtg gagatgttgt ccttcacccc
     7321 tatccgtatt ccggccagcc ggccttccct tccgggttcc ccatcgtacc gggtgatgaa
     7381 ggcgtggaac gggtcgtcct cggaaccggg gcctggggtc ttcactccat caccctcggt
     7441 gccttgatga acccctcctc gggttcggat gagagggaga ggacctctcc ctgggggagg
     7501 cagggagtga tctcatcctc ccgcatgaca ttgacctcgc ctcctgctgc cctggcctcc
     7561 tcccggacgg tgtccaggat atcgaagtac tcgaggatcg cgttgaactc ccgggtgaac
     7621 ccggaaagcc gctcccgggc gagcccgata tcggcgagcc ccgcgatctt ctccacgtcg
     7681 tcaggtgaga tcatgtcctt cccccagaag ctggatcaag tattctctca ccgacctttt
     7741 ataaccgttt ttacccgcga gtccgcggat ggcccccgcg atcccgctcc cgtactgcat
     7801 cgccttcttc ggcgcccggg cgaaatccgg cgtgaggagc ctcgccgcaa gctccctgag
     7861 cgggatcttt cccgttcctc cccggaggag ttccccgggt ggcagggcct gggcggcgcg
     7921 caccacccgg aggtccaggt acgggaggga aaaggaggtc ccgtgaaggc gtgccactgc
     7981 ctggtcgcgc agggcctgtc cacggagaga ggcgaagtcc cgggcgagca tccccgggat
     8041 gtccgcggct gtacggtacc gggcatatcc ggcgaagagt tcgtcggccc cctgtcccac
     8101 gaggatccgg cggtaccctc tccccgcggc ccaccgggcc acgaagaaga atcccgtcgc
     8161 gatggccacg tcgaggggag ccggccccgg gatggccccg gtgacccggg ggagggcggc
     8221 ctccagctcg tcctcggtga tggtcacccc ctcccaggag agcccgaggg cctgtgccgc
     8281 tgctcctcct ttctccaggt cccgggagcc ctccattccc accgtcacgc acgggaggcc
     8341 agccaggcag gcgatgaggg aggagtccac accgcccgag agggatacca ctccctggtc
     8401 ggcccggagt ctgactgccg tgcggagggc ctcctccagg ccgagcgtgg gtgaatccgg
     8461 attcacctcc ccgactgcat ttcccccgca ggtcacgatc ccgaagggcc cggggatacc
     8521 gaggatgccg agccggtcgg aggccgtgca cccgtcccag gtgaggtaaa actccccccc
     8581 gaatccagcc acccggccgg gatctgcccc gacaagggcc tcaacttcgg gaagggtgag
     8641 tttccttccg cccagctcta cccagccctg cagccggatc cggggactcc ccgtcatctc
     8701 cttcgggagg gggttttggg tgcaggagcc ttccggtcga ggtatttcga gagccgggcg
     8761 atgacccgtt ccacctggac gggggcggtg ccaatatact tcgccgggtc gaggagtccc
     8821 gcgagcgcct tccgggaaat gagtttcttc accgccgggt tcccggcaag gaccgtggca
     8881 aggtcatgcc ccttccggta ggcttccatg ctcgcctccc ggacgatctc gtgggcatcc
     8941 tgccggccca ttcccttctt cgtgagttcc accatcaccg attccgccat ggggatccca
     9001 tcgaggatgg tgaggttctt cttcacgttc ccggcgttga ttgtgagtcc atccataacg
     9061 gtggtcatca cctgcaggat gtggtcggca aggatggtcg cctcggggag gatgacccgt
     9121 tcgcaggagg agttggtgag gtccctctcg tcccagaggg tgttgtcctg gagggccggc
     9181 tccacggcag aacggatgac ccgggcgagc cccgagacct gctccgacct gatgggattc
     9241 ctcttgtggg gcatggtgct cgagcccacc tgtttttttc cgaagggctc ttccagttcc
     9301 gcgatctcgg tcctctggag cgtccgcacc gtgatggcca tccggtccag ggtggtcgca
     9361 aatccggcga ggaacatgat gtactcggca taccggtccc ggggcacgat ctggttcgtg
     9421 atatcgggga ttccgaggcc gagctctccc atcattttct cctgtacggc catgccgtcc
     9481 ttcccgaggg cggccatggt ccccacggcc ccggccatct ttcccacctc gaccctcggg
     9541 cgcatctccc ggagacggtc gacgtggcgg gcgatctccg aggcccagag ggcaaaccgc
     9601 atcccgtacg tggtggggac tccgatctgg ccatgggtcc gggcggcgca gaccagggtc
     9661 cgggtctccc ccgcacgccg gaggaggacc gagaggagac tgaggagctt cgtctcgagg
     9721 agggcgaggc tctccttcag ctggaggcct gtcgcggtat ccaggatgtc gttcgaggtt
     9781 gccccgaagt ggacccaccg gcccgcatcc cctgagacct cggacagggc cttcaccacc
     9841 gccatcatgt cgtgattgat ctcggcctcg atctcctttg cccggccgag ggaggccttc
     9901 tgtgcccccc gggcgatggc cttcgcatcc ttctcgggaa tgagcccgca gcgggcctcc
     9961 gcctgggcga gggccgcctc ggcccggagg atgcaggtga accggtgctc ctcggtccag
    10021 accgcccgca tctccggagt cccgtagcgc gattcgatgg ggtggatcgc catgaagagg
    10081 tgtcatcctg caagagtaaa aaggagtggg ggccaataga tcaccggaca tggacgccat
    10141 ctccgccttc ttcccgtacg aggcctaccg cccccaccag cggcagatgc tcgagctcgt
    10201 cgcccagacg gcggaaaaag gcgggatcgc catgatcgac gcccccacgg ggagcgggaa
    10261 gtcgagcgtc gtctcggcgc tcctcgccgg ccggagggat cggacggtgg taattgccgt
    10321 ccgaacggtc tcccagctcg ccaccttcgt gcgggagctc accctcgtgc ggaaacgccg
    10381 gcccgagctc cggttcgcct acctcatcgg caaggggcag atgtgccggc tcgggggcgg
    10441 ggggaacgtc taccggcggt gcgaggggct caagtccctc tccaccgccc tcatgcggga
    10501 ccgggcagaa gcgggttccc tggtccctgc ccgggaccgg ctcgtccgcc agcagatccg
    10561 gaagaccacc gtcgatcatc ccctgatctg tcccccgttc atccacagcc ggtcctttgc
    10621 ctcggaggag ggtgcgggcc tccggatggt cccgtccgcg gcgctgcgaa cccgggccga
    10681 gcgcgtgggg cgggagacga tctggcccga ggagctcccc gccgcctcgg gggactgctg
    10741 cccgtacgag gtgatgctcc atgccgcccg cggtgccgac gtcatcctcc tgaactacca
    10801 ccatgtcttc gacgagagca tccgggagca gctctacctc cagctcggca tcggcgcggc
    10861 cgaggtcctc ctcctcgtcg acgaggccca caacctcggg gatgcggtcc agtcggtcca
    10921 gtcggtctcc gtcgggtacc gggacctgga gggtgcggag tcagagctcc agcggctccg
    10981 gggcaaggtc cgggccgtcg aggccgtggg aagggtcttc ccccaggtga ggcgtttcat
    11041 ggagctcctc cgggattccc gggaaggaga ggactggttc gaccccgcca tcttcgagcg
    11101 catggtggtg aagggttccc tgtacgcgga catggaggcc atcgtcgatg acatgctctc
    11161 ggtctctgat gccatgaagg agagaaacct gaaggccggc gacttccggg agacgtccgt
    11221 ggagcgcctc accactttcc tttaccggat cttcctctcg gcccatgacc ccgcctacct
    11281 cacggtgtac cggcgggaag aggaggaggt catcctggag gtccggaaca tcgatccaca
    11341 ggcgaagatc ctggagctcg cccagtccca tgccgcgacg gtcctcatca gcgggaccct
    11401 ctcccccgcg gagtccttcc ggcgcctcta cttcggggac gagccggccg ccgttctcac
    11461 cctcccgaat gccttcccga cggagaaccg gaggatcgtc gtcgcgcggg acgtgaccac
    11521 cctgtacagc atgagggaca aggcggggaa caacgaccgg atcgcgggct acatcacgga
    11581 gttcagccgg ctcccgggga acctcgccgt ctacttcccc tcctataaga tcctctcgtc
    11641 cttcgccgac aaatgtgcgg gatccatccg ggggaaggag gtcatcgtcg agccccgcga
    11701 cccgaaggac gcgtcggccg cccttcggtc cttcctcacg cttccggagc agggaaggtc
    11761 gggggtgatc ttcgcggtct gcggcgggaa gtggagcgag gggctggatt accggggaga
    11821 gctcctgacg ggtgcaatgg tggtcgggct acccctcgcg ccgtacaccc gggtacgggc
    11881 catgatcatc gactacttcc ggaggaagtt cggggaggag ggagagtttc tctcctacac
    11941 cctcccggcc atgaaccg
//
LOCUS       DATGDI010000048         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_120990, whole genome shotgun sequence.
ACCESSION   DATGDI010000048 DATGDI010000000
VERSION     DATGDI010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120990"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VKO45_01855"
     CDS             complement(<1..277)
                     /locus_tag="VKO45_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HMA04649.1"
                     /translation="MDEREELKPLGQLLAQGKITQEEFDRARAKILAAGENLPCPACG
                     ASNPADAQTCFNCGVLLRGERFVCPDCGADLPSGDSTGCPGCGARFAA"
     gene            complement(307..1404)
                     /locus_tag="VKO45_01860"
     CDS             complement(307..1404)
                     /locus_tag="VKO45_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HMA04650.1"
                     /translation="MIFIIAILFCTLFVSVCPVLAVNGACVTVSQARQMAAAGKLRPE
                     YARMTDAQLYALGFCADLPGTTTAPATGCNAGYYKFTTSAGHCCPEGYPYFWGGSCYQ
                     CAEGYFIYDSSAGHCCKEGYPYYDSGTCYAQDPNGDGGNGTGDDDGFVGFLFLGMFVF
                     IFAALAGLVIRSNSRKNPPAPQEVMPGEGEKVPRKMHGLMQFGGDSPGRVITKGPGPI
                     PGNFHGPGPSNPAYGYEEPPQGGETRAGILPSLPSAEGGHPEPGMGIVSQAAIPQPPV
                     SSLKVSRAGNMVRLEWKPPAHDPAREQLVGYEVFRQEPVKMSNALQRVSLGTVDPGVN
                     GFEVAYQEGARFYNVRPIYRTPDGIAYGPGF"
BASE COUNT          299 a          488 c          447 g          304 t
ORIGIN      
        1 tcgcggcgaa cctcgcccca caccccgggc agccggtcga atccccggag ggcagatccg
       61 ctccgcaatc cgggcacacg aatctttcac cccgcaggag caccccgcag ttgaagcagg
      121 tctgtgcgtc cgcagggttc gatgcaccgc aggcagggca gggcaggttc tcccctgccg
      181 cgagaatctt cgccctggcc ctgtcgaact cctcttgtgt tatcttcccc tgggcgagga
      241 gctgcccgag cggctttaat tcttcccgtt catccacttc catcaccctc cggcgggaca
      301 ggggtttcag aaacccgggc catacgcgat cccgtcggga gtccggtaga tcggcctcac
      361 attgtagaac cgggcaccct cctggtatgc gacctcaaaa ccgttcacac ccggatccac
      421 ggtgccgagg gaaacccgtt gcagggcatt gctcatcttc accggttcct gcctgaagac
      481 ctcatacccg acgagctgtt cccgcgccgg atcgtgggcc ggaggtttcc attcgaggcg
      541 gaccatgttc ccggccctcg ataccttgag ggaggaaacg ggcggctgcg ggatcgcagc
      601 ctgtgagaca atccccatgc ccggctccgg atgccccccc tctgccgatg gtaacgacgg
      661 aagtatcccg gcccgggtct cacctccctg gggcggctcc tcataaccgt atgcgggatt
      721 cgatggcccg ggaccatgga aattccccgg gatcggcccg ggccctttcg tgatgaccct
      781 gccggggctg tccccgccga actgcatgag gccatgcatt ttccggggga ccttctcccc
      841 ttcaccgggc atgacctctt gcggagccgg cgggtttttc cggctattgg accggatcac
      901 gagcccggcg agggcggcaa aaatgaacac aaacatgccg aggaaaagga atccgacgaa
      961 cccgtcgtca tctccggtcc cattccctcc atctccgttc ggatcctggg cgtagcaggt
     1021 ccccgaatca tagtaagggt acccttcctt gcagcagtgg ccggcgctcg agtcgtagat
     1081 aaagtatccc tcggcacact ggtagcagga ccccccccag aagtagggat acccctcagg
     1141 gcaacagtga ccggcactcg tggtgaattt gtagtatcct gcattgcagc ccgtcgcggg
     1201 ggcggtggtg gtccccggga ggtcggcgca gaacccgagg gcatagagct gagcatcggt
     1261 catgcgggca tattccggcc tgagctttcc ggccgctgcc atctgtctcg cctgggagac
     1321 ggtcacgcag gccccgttaa cggcgaggac cgggcagacg gagacaaaaa gggtgcagaa
     1381 aaggatcgcg atgatgaaga tcatcctgat cttcctgtga tggcgggaaa tctggatcgt
     1441 accaaaccct ctctcgatat tggttatctt atgccacttt tatatataca tatcgttatg
     1501 agattcaaga tattttataa tctattaaga aaaagggg
//
LOCUS       DATGDI010000049         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_33971, whole genome shotgun sequence.
ACCESSION   DATGDI010000049 DATGDI010000000
VERSION     DATGDI010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33971"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VKO45_01865"
     CDS             <1..1022
                     /locus_tag="VKO45_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04651.1"
                     /translation="SVGSTDPTSYDDLNPDYVSFSGVSSQNMAAYAKANNIKIFTIGY
                     GAQLSAGGRDTLEKIASTTGGKYYYASTGDDLASVFTDIAGALKDTAGVATSVALDFS
                     KVEVTDVKGVQVGTGSSVFKYERIPWKSTLVTLPDKSQRDVNNSADWLTNKLNVSLGT
                     IKVNEEGVITFTLNVTKSGNIKLLGSSSKVNFLDVYGAPGVVPLPDTYLTAIPAGSDP
                     GVSTPTFLIWDVKRTNGETDPDTLAIAWRVTYSGDCEYDDGITVALTSIDLGSIGDQY
                     YFHAYPTHTPYDTGTYTLDISDLVPGRYNVRVRGQPCDVAYPSSNTTQFTILAPSQSP
                     QIVIR"
     gene            complement(1205..>2935)
                     /locus_tag="VKO45_01870"
     CDS             complement(1205..>2935)
                     /locus_tag="VKO45_01870"
                     /inference="COORDINATES: protein motif:HMM:NF044377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HMA04652.1"
                     /translation="LKYLPPNARIHFPPIDPTNPYDVGTTIDIEEVMTQIQQVDTIEI
                     DAILLFAGLGGSMVDTAPLIIPELRKSFIEPVFGVVTLPCGGEGKKRSAKAADDIEHL
                     KPLVDAVILFDNDTWYRKIKDELAITDEKVAKREIPKRLAKKILSQPLNRKEYIHNLL
                     NTRIGRQMGLILHAGEFSEEGVEVGEVVLDAGEVLNTLVGMGYVAIGYAAENLPSGYL
                     KILDRWRPASYFIQNSQTKASRIVSLAKKAIYEEVSIPCDLTSADKALVLIAGPSQEL
                     SMRGFQTVRKWIDRSIAGLEMRSGDYPIQNTRYVGIIIMLSGMHNIPRLDELAQAREE
                     YRMELEQEKPRTEEELPGSGTETGVPVEVVGTGAASAAPDGAAWEKKDRMITVPGSRK
                     AQKGGRPAPLFEGLNMSIGKVRGIGETQGREGGAPADITRKTVVKAGQAPKDTVFGLK
                     DVRVERQAGKAAGDRSGMEIPRAARPREDALGPGEIRTGQKGTARDSTFTTRNVQVRE
                     TGIRPIDSTLLTPGKGKVPAAGKARELDPGKRRSEILEPPAAGKEAGKPERKPRSRGK
                     KDDEEIGWIH"
BASE COUNT          509 a          943 c          886 g          597 t
ORIGIN      
        1 gctcggtggg ttccacggat cccacgtcat acgatgacct gaacccggac tatgtctcct
       61 tcagcggggt ctcctcccag aacatggcgg cctacgcgaa ggcgaacaat atcaagatct
      121 tcaccatcgg gtacggggcc cagctctctg caggcggcag ggacacactc gagaagattg
      181 cttccaccac gggaggcaag tactactatg catcgacggg agacgatctc gccagcgttt
      241 tcacggatat cgccggagcc ctgaaggaca cggccggggt ggccacgagc gttgcgctgg
      301 acttctcgaa ggtggaggtg accgacgtga aaggggtcca ggtcggcacg ggctcgagcg
      361 tctttaagta cgagcggatc ccgtggaagt cgaccctcgt gacattaccc gacaagtctc
      421 agagggatgt gaacaacagc gccgactggc taaccaacaa gctgaacgtg tcactcggga
      481 ccatcaaggt gaatgaggaa ggtgtcatca ccttcaccct gaacgtgacg aagagcggga
      541 acatcaagct cctgggaagc agctccaagg tcaacttcct ggacgtttac ggtgccccgg
      601 gcgtggttcc cctgccggac acctacctca ccgcaatccc ggcgggctcg gacccggggg
      661 tgagtactcc gaccttcctg atctgggatg tcaagaggac gaacggcgag accgacccgg
      721 ataccctcgc catcgcctgg agggtcacct acagtgggga ttgtgagtat gacgatggga
      781 tcaccgttgc acttacaagc atagacttgg ggagcatagg agatcagtac tacttccatg
      841 catacccgac tcatactcca tacgacacag ggacatacac cttggatatc agtgatctgg
      901 tccccggaag gtacaacgtg agggtccggg ggcagccctg cgacgtggcg tacccctcct
      961 ccaataccac acagttcacc atcctggccc cgtcacagag cccgcagatc gtcatccgtt
     1021 aaatccgaaa cccctttccc ttttctctcc ttcgatatcg atactcccgg agaggacagg
     1081 gatcgggcgg gatcgggctc cagggaatcc acccccaatc ggatgtgaat cttcctttcc
     1141 agccacctgg tggctgaccg ggaataagcg gaaatctgcc catcggaaag gcagagagaa
     1201 aaatctagtg gatccagccg atctcctcgt cgtccttttt gccccggctc cggggcttcc
     1261 gctcgggttt ccccgcctcc ttccccgcgg caggcggttc gaggatctcg ctcctccgct
     1321 ttcccgggtc gagctccctg gcctttccgg cggccggaac cttccccttc cccggggtga
     1381 ggagcgtgct gtcgataggc cggatccccg tctcgcgcac ctggacgttc cgggtggtga
     1441 aggtggaatc cctggccgtc cctttctggc cggtcctgat ttcaccgggg ccgagggcat
     1501 cctcccgggg cctcgctgcc cggggaatct ccatgcctga tctgtctccg gcggccttcc
     1561 ctgcctggcg ctcgacccgg acatccttca ggccgaagac ggtgtccttc ggcgcctgtc
     1621 ccgccttcac cacggtcttc cgcgtgatat cggcgggggc acctccctcc ctgccctggg
     1681 tttccccgat cccccggacc ttcccgatgg acatgttgag cccctcgaag aggggtgccg
     1741 gccggcctcc tttctgtgcc tttctcgacc ccggcaccgt gatcatccgg tcctttttct
     1801 cccaggcagc cccgtcggga gcggctgaag cggctcccgt tccgaccacc tccaccggga
     1861 ccccggtttc cgttccggat ccggggagtt cctcctctgt ccttggcttc tcctgctcga
     1921 gctccatccg gtactcctcc cgtgcctggg ccagttcatc gaggcgcggg atgttgtgca
     1981 ttcccgagag catgatgatg atcccgacat agcgggtgtt ctgaatcggg tagtcccctg
     2041 agcgcatctc gaggccggcg atggaccggt cgatccattt cctcacggtc tggaagcccc
     2101 gcatggagag ttcctgggac ggcccggcga tgagcaccag ggccttgtcg gcgctcgtca
     2161 ggtcgcacgg gatggagacc tcctcgtaga tggccttctt cgcgagggag acgatcctcg
     2221 aggccttggt ctgggagttc tggatgaagt agctcgccgg ccgccaccga tcgaggattt
     2281 tcaggtaccc gctcggaagg ttctcggcgg cgtacccgat ggcgacatac cccatcccga
     2341 ccagggtgtt cagcacctcg cccgcatcga gaaccacctc cccgacctcg accccctcct
     2401 cagagaactc cccggcatgg aggatcagcc ccatctgccg gccgatgcgg gtgttgagga
     2461 ggttatggat gtactccttc cggttcaggg gctgggagag gatcttcttc gcgaggcgct
     2521 tcgggatctc ccgctttgcc accttttcgt cggtgatggc gagctcgtcc ttgatcttcc
     2581 ggtaccaggt atcgttgtcg aagaggatca cggcgtcgac gaggggcttg aggtgctcga
     2641 tgtcatcggc ggcctttgcc gagcgtttct tcccctcccc gccgcagggg agggtgacca
     2701 ccccgaagac cggctcgatg aaggacttgc gaagctcggg gatgatgagg ggggcggtgt
     2761 ccaccatgct ccccccgaga cctgcaaaca ggaggatggc atcgatctcg atggtgtcca
     2821 cctgctggat ctgggtcatc acctcctcga tatcgatggt ggtcccgacg tcgtacgggt
     2881 tcgtcgggtc gatggggggg aagtggatcc gggcattcgg ggggaggtac ttcag
//
LOCUS       DATGDI010000050        15080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_295600, whole genome shotgun sequence.
ACCESSION   DATGDI010000050 DATGDI010000000
VERSION     DATGDI010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 15080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15080
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295600"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VKO45_01875"
     CDS             <1..1330
                     /locus_tag="VKO45_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HMA04653.1"
                     /translation="FLNKDLTSGNEDNGKGSALSKVAKLPDGRIIMTLPEYRAVEYLM
                     IRDKVDLGVLKPYLNDRWIEDVTCDGLGPIFIEHKIFRGLKSIIEFHKSEELDQFVVK
                     LGERLKKPLTYRTPIVDATLPDGSRINIVYGTEISKRGSNFTIRKAMAEDVISVLRLV
                     EFGTCNYEMAGYFWICIEHGMSMFMSGETASGKTTSLNAVTAFIPPEHKIVSIEDTPE
                     LQVPHKNWTREVSKAKGKGEGEGADVTMFDLLKSALRQRPNMIIVGEIRGVEGSVAFG
                     AMQTGHPVLSTFHAASVEKLIQRLCGDPINIPKTFLDNLNIVVIQSAVKRPSGETVRR
                     MLSVNELVGYNPDTGGISFVEMFKWNPVNDTFEFTGKGASFLLENKIATMLGFPDSRK
                     AEIYDEVDKRARILERLHRAGYTDYRDLFQMIVKVKKEGLIRIGATPEVD"
     gene            1333..2937
                     /gene="flaJ"
                     /locus_tag="VKO45_01880"
     CDS             1333..2937
                     /gene="flaJ"
                     /locus_tag="VKO45_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellar assembly protein FlaJ"
                     /protein_id="HMA04654.1"
                     /translation="MAGTFGGFTEAIRAKTQGKIPLGEGLAGIRRFIDRIDENKRMPS
                     DLLFMATYMASITTAGVSRPDIFSYTSRRYEYVPSRYVQKADYFVKRWHYSYSEALKI
                     VAKGMKNEVLQSVFNRYANSIESGVPDEEFLDRELLTIRSVYRNSVEQGIEMVKKWGD
                     AYIAMLFSGVLVALIIMLSVAIFSPGGVESTLNLSYFIVIFIAVFGLVTMYRSMPEDR
                     RTHGLRDSCSREQATIRRMERPLLVVTGLGCATLWFLGMGPGFVYLLAGILLAPLGIL
                     GFIDDGHIMGRDTDFPTFIRTLGSVMGGKGIPMVQGFLEVDRASLPKIRDLVDSIYSK
                     LNLGLDEDQSWKRFIGESGSNNIYKYTNIFRDSVTLGGSPDKVGLIVGTSSLEQVLLR
                     DKRHVVAMGFVTLLVSMHAAMVGILLFLFYIMKTISESMTAMMSTLGESQAAIESGGG
                     SLMPSGLNVSFLVNFPVQTMTVYVMIIIFLITLSNVIAGRIVYGGDRYIFYLFATLLF
                     SISGVIAIVAPEIVSLFFKISLPGAM"
     gene            2939..3757
                     /locus_tag="VKO45_01885"
     CDS             2939..3757
                     /locus_tag="VKO45_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04655.1"
                     /translation="MHKTVRQMILGLGTFGTGTALLALEVNPYLLVLADTVVGVGMLF
                     AAGSLTVDDLHLRRSATPVKEGLIDEIATPAGGGPEKSPGPTDSLGKLGGALGHLGRG
                     KEQRETRAKEIDKMLDTTLAGQSRRLISLAEGQGAVPGAAGATAGAPKIEEDLLQGIS
                     GGAISDQFPGDEADEEDEMATPARELLSTVAPGSGAAAGESGRATEGARADVLSLSDA
                     ALGSDDLLSALRLEAMREKKKDDTSLLRNLKGVRVTGRQLLDELDSLVREMRGR"
     gene            3747..4985
                     /locus_tag="VKO45_01890"
     CDS             3747..4985
                     /locus_tag="VKO45_01890"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HMA04656.1"
                     /translation="MADRPEICRIRGDGMGRFTAAGILALLCGSLLLAAIPPVSAEME
                     IFSGSSQLSSMEIHDMTQDPTGNVYFGTDNGLSIYDGSWHITHREYRTGDGGLLSDYI
                     LALGFDSGGNLWIGFPNGLQRLEAGTYVTMKDQQVLKSLEIHGLLRRGREMWVAAGSS
                     GIHRYIDGTWTWFSPGGPGGLGCGYVTSMATDPVTDTLFVACKEGIWSLNGTGRSLSF
                     SPVVNPGLIPGPVRGIVGDPFGGVYLLYASTILHLTPPGQWSAAVTTTDLTPGIAIND
                     LRVDARQTLWIATDNGIYAWKDGRVRLHLDSTSGIRNNAVQQLFLDSSGRLWFVTPEN
                     VGFYRVETSPGEEGGTIPVITYEVPGGTSPEVSLTAPVSQVTPGISVQGFPEETTGSQ
                     GGLPGFLDALLGFLRGLFGR"
     gene            complement(5156..6370)
                     /locus_tag="VKO45_01895"
     CDS             complement(5156..6370)
                     /locus_tag="VKO45_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04657.1"
                     /translation="MQDEQLSGLTLDEKLRKVRGEFLDYIDKNIKEISATLPVFFAHI
                     ITMLNDSFPGLKDEVHDEFIDAITYRVLAASRQGGNLPFVEKVSANAVRAKRGPRAKQ
                     GTDIIAGMELIKSENYAPAARLLKEYSHLDAIIGAAVAYCYYMLSMREIASLREKGTR
                     IDEKERRPNEFELLAREEMLRLANSRPPLGAVRELRIRGEEEPIIRRAFWLMISCSFE
                     WFPNEKGFILVGLEKAKRDRNWEMKNELLKIATERFFDDFFFMKEMYHFRLGAKDGTG
                     AAAVVRQMMQQFPDGLEPLYFGLQISMLSLKKTTYDGFRKLLLMKGIPEHIPALVDVA
                     YQVMDGDPRQGLASLKELAGKFPRFDFYFLSLEYLLKETSSEDPKRNRRARKVFLDSI
                     DRFCMKELGIER"
     gene            complement(6854..8626)
                     /locus_tag="VKO45_01900"
     CDS             complement(6854..8626)
                     /locus_tag="VKO45_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HMA04658.1"
                     /translation="MRIAVVHRDRCHPKKCGTECILYCPRVRTGDETVVIGEAGTAVV
                     SEELCVGCGICIKKCPFSAIDIVSLPEELPHPTHRYGVNGFALYGLPVPVRGKVTGLL
                     GPNGIGKSTSVQILSGGLRPNLGEPGREVPWKEILSHYTGTELFEYLRTVSEGRLKTA
                     TKPQYIDYIPRMFRGTVGDLLKTTDERGQLARYLEAFSLTPLLDRPIDSLSGGELQRV
                     AICACLSREADFYFLDEITPFLDIYQRMAAATLIRELALERPLMIVEHDLAILDLLAD
                     TVHIAYGKPAVFGIITQPKGVRVGINQYLEGYLAEENMRFRDYPVVFERRLHGERPEL
                     EPLTEIPPMVKRYPGFTLEVGGGAIHRGEILGMVGPNGIGKTTLARLLAGEEKPEVGK
                     MPAALTISYKPQYLKADSELTVEEILRGTTNRFDSSFFQHEVLEPLSLAPILASPEKT
                     LSGGELQRLAIAACLSREADLYILDEPSAHLDVEQRVKLPRVIRNLAQEREAGILVID
                     HDIYLIDIISEGLMVFSGDPGRHGLAEGPFPMREGMNRFLETLGITFRRDRSGRPRIN
                     KPGSFLDREQRATGTYYYSEISKG"
     gene            complement(8633..8977)
                     /locus_tag="VKO45_01905"
     CDS             complement(8633..8977)
                     /locus_tag="VKO45_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04659.1"
                     /translation="MAAEAKPEPEKTPKKKLTKAEKQAQHMDRLKKTAIACGMGILVG
                     FLSFRIETPPTGTALGILFLLAGIVFQKYLFIALRMDYLGLTAKDWLYQGFMTFSLWF
                     ITWTILLSAPTG"
     gene            9035..10921
                     /gene="rqcH"
                     /locus_tag="VKO45_01910"
     CDS             9035..10921
                     /gene="rqcH"
                     /locus_tag="VKO45_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue protein RqcH"
                     /protein_id="HMA04660.1"
                     /translation="MATAQGMSGVDLSAVAAELLPLLPLWVGKIYQYGPKTLAIRLQG
                     EDRARHPLVIESGRRVHLVRTLPEAPERPGGFAMLLRKHLEGGRVLGIAQHGLQRILT
                     LTVGKKAGSLRLVIELFDEGNVILVDEGGKIVKPLWHHRFREREVVPGADYEFPGNDC
                     TDLSPGSFGELLRSSDREIVKALAVDCMLGGRYAEEVCAMAGIGKDTPAGSVDPVAVH
                     AAFMALLERAKTGRDPAITPSGCWPFPLSGEPVTSRFPSYEEALEAYYGGPAPSQAKE
                     SRVRAKREENIRNRQESSLRKFEDEIRRREGAIERIYENYSLVEDVIRALSQAEKKTS
                     WEEIEKTLRASTLPSAKAVVKFHPESASVTLDLGEKVKISIRESVRENIGRLHEEIKK
                     FRKKREGALAALARPLPAPPEKRERAAEGKRRWFNRFRWFETSDGVLVVGGRDAGQNE
                     ELVRKYMAGSDTFVHADVHGAPVLIVKGATEHMDEVAQFAASYSGAWKSGHATADVYS
                     VRPDQVSKAAPSGEYLSTGGFTIRGERTWFRDVPLAVSIGLAEGDSPRVIGGPPGAVV
                     KKATHRVTLRPGVFEPNDIAKKVVRALREMIPEAERGGLRRVLSTESVAAFVPPGGSE
                     VVGE"
     gene            10918..11943
                     /locus_tag="VKO45_01915"
     CDS             10918..11943
                     /locus_tag="VKO45_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein pelota"
                     /protein_id="HMA04661.1"
                     /translation="MKAEFLEMKGSHGEIRLFPESVDDLWHLSHLIRPGDLVFATTFR
                     SVETVQDKIRPEKPEKKPVRLGIRVEGVAFHPYANRLRISGVIQHGVDEGSHHTINLD
                     AGTEISVIRTWRQVDLERIDRAVKATSAGAVHILAVEEGEAELYRIRQYGPELVSTVI
                     QGSSKGADTGARGILFERVATLLAEVTGTVVIAGPGFVKDEAAKYLKEKIPDLRDRCI
                     TVETRRSGRGAVTEALGKGVLERITGDLQLKREAELLDLFLERMGKGEPVAYGMTEVE
                     EAVSAGAAEHVLVIDEMVRQRHVADLLERAERSRARVTVFSSLFDPGQQLVALGGIAA
                     ILRYRMK"
     gene            12014..13432
                     /locus_tag="VKO45_01920"
     CDS             12014..13432
                     /locus_tag="VKO45_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HMA04662.1"
                     /translation="MGTDSIVFHGTLRQLLEKVETGEMVPKLQASHAPMCKFFTAYYV
                     ISGIRHVVPLVHGPTGCPLYVSDFVRTRECCEIRGVPLEPTACTSLDESHVIYGGEGK
                     LLEAVKEAYAKYNPDLIVILSCCCSGIIGDDVESIAREAEKLVGCRVLALRSEGFGGD
                     FRSGYEDAFKLIMELMEPPREKRKRTVNILGARWGPTTTEFDMDLDEVERILHEVGIQ
                     VNAVIAGGCTVEELRRAREVELNASWCYDWGQKLGDLMEERFGIPYSKTGQPYGLEAT
                     KEWLLGIAKPLGMEEKALEVIEREAREVEPDLEYLKQALEGKTAIIEITEFPGPIRAL
                     SLARMAGEFGAHPVVINIHPYTIRERMPSIKFLLETGVNPEIILTKGLFRLGSFRSSK
                     ETEDELEEIVRGYDDPIFFGNAARYPPCPVVNLTLMNPTFQPQYGFRGIRNIAALVRQ
                     ALEKKDLPRSRLLRGMLYGTRR"
     gene            13416..14774
                     /locus_tag="VKO45_01925"
     CDS             13416..14774
                     /locus_tag="VKO45_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HMA04663.1"
                     /translation="MGPGDRMSPAAQDEGTGEKCTDPYLRCAFNGAAQTALGVSGSAL
                     LAHSPQGCEYLVNNAFAWQECDYMETITLCTKLCVDEIVHGGEDLLARTIREAKGLPI
                     SALFVASACGPEIVGDDIVAVCEAVQPEVKFRLIPIESAGFRGSQYDGIDIALDAILS
                     QLVRRDAEKVPGSVCLIAPHANANPTWPADLQWVKQTLSRLGVPVIATLTHRTPLAEI
                     ERASSAETSLLLSHDAGQKAADRLASEYRIEQVCRGIPLPIGMTNTARWLAELGRRFD
                     RVKEAEQMVAEGERMVMTTCRRKWPMARFFFRTPAAIVADATVGIPLLRFVTEDMEMT
                     PDLIALYTGHRQVRELLEGELADLGLKPKVVYGTDVYKTRRGIGEVRPRVVFGSTIER
                     HASEGLGVAYVVELVRMMRQFRFIDREYFGYTGILNLFEAVQNEWIMRWRSKEKRYEA
                     KW"
     gene            14782..>15080
                     /locus_tag="VKO45_01930"
     CDS             14782..>15080
                     /locus_tag="VKO45_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMA04664.1"
                     /translation="MRQIAIYGKGGIGKSTIASNVSAALAEMGLSVMQVGCDPKRDST
                     RILAGGDLIPAVLETYREQLRVGRDEYAMKLDTIVRKTPQGIYLVEAGGPEPGVG"
BASE COUNT         2795 a         4699 c         4976 g         2610 t
ORIGIN      
        1 cttcctcaac aaggacctga cctcgggtaa cgaggacaat ggcaagggtt cggccctctc
       61 caaggtcgcg aaactccctg atggccggat catcatgacc cttccggagt acagggccgt
      121 cgagtacctg atgatccgtg acaaggtcga cctgggggtc ctcaagccct atctcaatga
      181 ccggtggatt gaggatgtca cctgtgacgg gctcgggccc atcttcatcg agcacaagat
      241 cttccggggc ctcaagtcca tcatcgagtt ccacaaatcc gaggagctcg accagttcgt
      301 ggtcaagctc ggggaacggc tgaaaaaacc cctgacctac cgcacgccca tcgtcgatgc
      361 gaccctgccg gacgggtccc gtatcaacat cgtgtacggg accgagatct cgaaacgggg
      421 gagcaacttc accatccgga aggccatggc agaggatgtc atcagcgtgc tccggctcgt
      481 cgagttcggg acctgcaact acgagatggc cggctacttc tggatctgca tcgagcacgg
      541 gatgtccatg ttcatgagcg gcgaaacggc cagcgggaag accacgagcc tgaatgccgt
      601 gacggccttc atccctcccg agcacaagat cgtctccatc gaggacaccc ccgagctcca
      661 ggtcccgcac aagaactgga cgagggaggt gtcgaaggcc aaggggaagg gcgaggggga
      721 aggtgcggac gtgaccatgt tcgacctcct caagtccgcg ctgcggcaga ggccgaacat
      781 gatcatcgtg ggggagatcc ggggagtgga ggggtcggtc gccttcggcg ccatgcagac
      841 cggtcacccg gtcctctcca ccttccacgc ggcctcggtg gagaagctca tccagcggct
      901 ctgcggggac cccatcaata tcccgaagac cttcctcgat aacttaaaca tcgtcgtgat
      961 ccagagcgcg gtcaagcgcc cgtcggggga gaccgtccgc cgcatgctct cggtgaacga
     1021 gctcgtcggg tacaacccgg acaccggggg gatctccttt gtcgagatgt tcaagtggaa
     1081 cccggtgaac gacaccttcg agttcaccgg gaagggggcg agcttcctcc tcgagaacaa
     1141 gatcgcgacg atgctcgggt tccccgacag ccggaaggcg gagatctacg acgaggtgga
     1201 caagcgcgcc cggatcctgg agcggctcca ccgggcaggc tacaccgatt accgggacct
     1261 cttccagatg atcgtgaagg tgaagaagga ggggctcatc aggatcggag ccacccccga
     1321 ggtcgattga agatggcggg cacctttggg ggtttcaccg aggcgatccg ggcaaagacc
     1381 caggggaaga tccccctcgg ggagggcctc gcagggatcc ggaggttcat cgaccggatc
     1441 gacgagaata agaggatgcc atcggacctc ctcttcatgg ccacgtacat ggcctccatc
     1501 accaccgccg gggtctcccg ccccgatatc ttctcctaca cctcgaggcg gtacgagtac
     1561 gtcccgtcca gatatgtcca gaaggcggat tactttgtca agcgctggca ttacagctac
     1621 tccgaggccc tgaaaatcgt cgcgaagggc atgaaaaacg aggtgctgca gtcggtgttc
     1681 aaccggtacg cgaactccat cgagtccggg gtcccggacg aggagttcct ggatcgcgag
     1741 ctgctcacca tccggagtgt gtacaggaac tccgtggaac aggggatcga gatggtcaag
     1801 aagtggggcg atgcgtacat cgccatgctc ttctccgggg ttctcgtcgc cctcatcatc
     1861 atgctctcgg ttgccatctt ctccccggga ggggtcgagt caaccctgaa cctctcgtat
     1921 ttcatcgtca tcttcatcgc cgtctttggc ctggtcacca tgtaccggtc gatgccggag
     1981 gaccggagga cccatggcct ccgggacagc tgctcccggg aacaggccac catccgccgg
     2041 atggagcggc cgctcctcgt cgtcaccggg ctcggctgcg ccacgctctg gttcctgggg
     2101 atggggccgg ggttcgtcta cctcctcgcc gggatccttc tcgcccccct ggggatcctc
     2161 ggcttcatcg acgacggcca catcatgggc cgcgacaccg acttccccac cttcatccgg
     2221 accctcggat cggtgatggg ggggaagggg atccccatgg tccaggggtt cctcgaggtc
     2281 gaccgtgcct ccctcccgaa gatccgggac ctcgtggact ccatctactc caagctgaac
     2341 ctcggcctcg acgaggacca gagctggaag cggttcatcg gcgaatcggg gagcaacaac
     2401 atctacaagt acacgaacat cttccgggac tcggtcaccc tgggaggttc ccccgataag
     2461 gtcggcctca tcgtcgggac atcgagcctc gaacaggtgc tcctccggga caagcggcac
     2521 gtggtcgcca tggggttcgt tactctcctc gtctccatgc acgccgccat ggtgggcatc
     2581 ctcctcttcc tcttctacat catgaagacg atatcggaat ccatgaccgc gatgatgtcc
     2641 accctcgggg agtcccaggc tgccatcgag agcgggggtg gctctctcat gccctcgggg
     2701 ctgaatgtca gcttcctcgt gaacttcccg gtccagacga tgaccgtcta cgtcatgatc
     2761 atcatcttcc tcatcaccct ttcgaacgtc atcgcaggaa ggatcgtgta cggcggcgac
     2821 cgctacatct tctacctctt cgcgacgctc ctcttcagca tcagcggggt gatcgccatc
     2881 gtcgcgcccg aaattgtcag cctgttcttc aagatatcct tacccggagc tatgtagtat
     2941 gcacaagaca gtacgccaga tgatccttgg cctgggaacc ttcggtacgg ggacggccct
     3001 cctcgccctg gaggtcaatc cctacctcct cgtcctcgcg gacaccgtgg tcggagtcgg
     3061 aatgctcttt gcggcgggct cgctcaccgt ggatgacctt cacctcaggc ggagcgcgac
     3121 accggtcaag gagggactga tcgacgagat cgccaccccg gccggagggg ggccggaaaa
     3181 atcccccggg cccacggact ctctcgggaa gctcgggggc gccctcgggc atctcggcag
     3241 gggaaaggag cagagggaga cccgggcaaa ggagatcgac aagatgctcg acaccaccct
     3301 cgccgggcag tcgaggcggc tcatctccct cgccgagggt cagggagccg tcccaggcgc
     3361 cgccggggcc acggccggtg ccccgaagat cgaggaggat ctcctccagg gaatctccgg
     3421 cggagccatc tcggatcagt tcccggggga cgaggccgac gaggaggatg agatggccac
     3481 gccggcgcgg gagctcctct cgacggtggc gcccggcagc ggggccgccg ccggagagtc
     3541 cgggagggcg acggaggggg ccagggcgga tgtcctttcc ctgtctgatg ccgccctggg
     3601 atcggacgac ctcctctctg ccctcaggct cgaggccatg agggagaaga agaaggatga
     3661 cacgagcctc ctccggaacc tgaagggggt cagggtgacc ggccggcagc tcctggatga
     3721 actcgattcc ctggtcaggg agatgcgtgg cagatagacc ggagatctgc cggatccggg
     3781 gagacggaat gggccggttc acggctgcag ggattctcgc cctcctctgc gggagcctcc
     3841 tcctcgcggc gatcccgccc gtctcggcag agatggagat cttttccgga tcctcccagc
     3901 tttcctcgat ggagatccac gatatgaccc aggatcccac gggaaacgtc tatttcggga
     3961 ccgataacgg cctctccatc tacgacggtt cctggcacat cacccaccgg gagtaccgga
     4021 cgggggacgg ggggctcctc tccgattata tcctggccct cggattcgac tcgggaggaa
     4081 acctctggat cggtttcccg aacgggctcc agaggctgga agcggggacc tatgtgacca
     4141 tgaaagatca gcaggtcctg aaatccctcg agatccacgg actcctgcgg cggggcaggg
     4201 aaatgtgggt cgcggcgggg agctcgggaa ttcaccggta tatcgacggg acctggacct
     4261 ggttctcacc cggcggcccc gggggcctcg ggtgcggcta cgtcacctcc atggccacgg
     4321 acccggtgac ggatacgctc ttcgtggcct gcaaggaggg gatctggtcc ctgaacggga
     4381 caggccgatc cctgtccttc tccccggtcg taaacccggg tctcatcccc gggcccgtga
     4441 gagggatcgt gggtgatccc ttcggagggg tctatctcct ttatgcatcc accatcctcc
     4501 acctcacccc tcccggccag tggagtgccg ctgtgactac cacggatctg acccccggga
     4561 tcgccatcaa cgacctccgg gtggatgccc gccagaccct gtggatcgcg accgacaacg
     4621 gcatctacgc atggaaggac gggcgggtaa ggctccatct cgacagcacc tcggggatcc
     4681 ggaacaatgc ggtgcaacag ctctttctcg acagttccgg ccggctctgg ttcgtgaccc
     4741 ccgagaatgt gggtttctat cgggtcgaaa catccccggg tgaggagggg ggcactatcc
     4801 cggtgatcac ctacgaggtc ccgggcggga cctccccgga ggtatccctc accgcgccgg
     4861 tgtcccaggt cactcccggg atttccgtgc agggatttcc cgaggagaca accggctcgc
     4921 aggggggcct gccgggattc ctggatgccc tcctgggctt cctccgcgga ttattcggcc
     4981 gctagaacgc ctcctccgtg cggccgtcgg gcgctaatct ccgggggggc gatccgactc
     5041 tgatcgaagg ggagggctgg atcccggggc gatctggcgc ggatctaggg ggcgatgaag
     5101 gctaagggcg gtggggcgcg gatctaaggg cgatctggcg cggatctaag ggcgattacc
     5161 gctcgatgcc cagctccttc atgcagaacc ggtcgatgga atcgaggaac accttgcgcg
     5221 cccgcctgtt ccgcttcggg tcctccgaag aggtttcctt caggaggtac tcgagggaga
     5281 ggaagtagaa gtcgaaccgg gggaatttcc ccgcgagctc cttcaggctg gcaaggccct
     5341 gccggggatc gccgtccatg acctggtagg ccacgtccac cagggcgggg atgtgctcgg
     5401 ggatcccctt catgagaagg agcttcctga acccgtcgta ggtagtcttt ttgagggaga
     5461 gcatggagat ctggaggccg aagtagaggg gctccaggcc atcggggaac tgctgcatca
     5521 tctggcgcac caccgccgcc gcgccggtgc cgtcctttgc cccgagccgg aagtggtaca
     5581 tctccttcat gaagaagaaa tcgtcgaaga agcgctccgt ggcgatcttc aggagctcgt
     5641 tcttcatctc ccagttccgg tcccgcttcg ccttctcgag accgacgagg atgaagcctt
     5701 tctcgttcgg gaaccactcg aaggagcagg agatcatgag ccagaaggcc ctccggatga
     5761 tgggctcctc ctccccccgg atccggagct ccctcacggc gccaagggga ggccttgagt
     5821 tggcgaggcg cagcatctcc tcccgggcca ggagctcgaa ctcgttgggc cggcgctcct
     5881 tctcgtcgat ccgggtcccc ttctcccgga gggaggctat ctcccgcatg gagagcatgt
     5941 agtagcagta ggccaccgcc gccccgatga tggcgtccag gtggctgtac tccttcagga
     6001 gcctggccgc cggggcatag ttctcgctct tgatgagctc catcccggct atgatgtctg
     6061 tgccctgctt cgccctcggc cccctcttcg cccggacggc gttcgcggag accttctcga
     6121 cgaaggggag gttccccccc tgacgggatg cggcgaggac ccggtaggtg atggcgtcga
     6181 taaactcgtc gtggacctcg tccttgaggc ccgggaagga gtcgtttagc atggtgatga
     6241 tgtgggcgaa aaaaacggga agggtggccg agatttcttt tatattctta tcgatatagt
     6301 ccaggaactc tcccctgacc ttcctcagct tttcatcgag ggtgaggccg gagagctgct
     6361 catcctgcat catgtagatg tatccggcgg aagatatatg aacctttagt gaaaattgcc
     6421 cgggaacggc ctcacgccct gctgggcaag cccctgactg aggaaggggc gggccggaga
     6481 aaggcctcag ccctacgaga ttgagcctgc ccgaagggga ggctcaatga gaaaggcgtt
     6541 agcttttcga gatttgactg tcccgccagg gacaggagaa ggagaaaagc agcagctttt
     6601 cgatacgcct cgcctccgaa ggagcgggcg tcgagaaaag cgctagcttt tcgagattcg
     6661 actgtcctgt aaaagacagg agaacgagaa gggcatcagc ccttcgagat tcgactgtcc
     6721 tgtaaaagac aggagaatga gaagggcatc agcccttcga ttttgagcct gcccgaaggg
     6781 gaggctcatg gagaagggcg ctagcccttc gagatttgac tgtcccgaga gggacaggag
     6841 aacgagaaag acgttagcct ttcgagattt ccgagtagta gtacgtgccg gtcgccctct
     6901 gttcccggtc caggaacgag cccggcttgt tgatccgcgg tctcccgctc cggtctctcc
     6961 ggaaggtgat ccccagggtc tcgaggaacc ggttcatgcc ctcccgcatg gggaaggggc
     7021 cctctgcgag gccgtgcctc cccgggtccc ccgagaagac catgagcccc tcgctgataa
     7081 tgtcgatgag gtagatgtca tggtcgatga cgaggatccc ggcctcccgc tcctgggcga
     7141 ggttgcggat gaccctcggg agcttcaccc gctgttcgac gtccaggtgg gcgctcggct
     7201 cgtccaggat gtagaggtca gcctcccggg agaggcaggc ggcgatggcg aggcgctgga
     7261 gctccccgcc ggagagggtt ttttcaggcg aggcgaggat gggggcgagg gaaaggggct
     7321 caagcacttc gtgctggaag aaggaactgt cgaaccggtt cgtggtgccc ctcaggatct
     7381 cctccaccgt gagctctgag tcggccttga gatactgggg cttgtacgag atggtaaggg
     7441 ccgcgggcat cttccccacc tcgggcttct cttcgccggc gaggagacgg gcgagggtgg
     7501 tcttcccgat cccgttcggg ccgaccatcc cgaggatctc cccccggtgg atggccccgc
     7561 cccccacctc cagggtaaag cccgggtacc gcttcaccat gggcgggatc tcggtgaggg
     7621 gctcgagctc gggcctctcc ccgtggagcc gtctctcgaa gaccaccggg tagtcccgga
     7681 aacgcatgtt ctcctcggcg aggtacccct ccaggtactg gttgatcccc acccgcaccc
     7741 ccttcggctg ggtgatgatg ccgaagaccg ccggtttccc gtacgcgatg tgcacggtat
     7801 cggcgaggag gtccaggatg gcgaggtcat gttcgacaat catgaggggc cgctcgaggg
     7861 cgagctcccg gatgagggta gcagccgcca tccgctggta gatgtcgagg aagggggtga
     7921 tctcgtccag gaagtagaag tccgcctccc tcgagaggca ggcgcagatg gcgacccgct
     7981 ggagctcccc tcccgagagg gagtcgatgg gccggtccag gaggggggtg agggagaagg
     8041 cctccaggta ccgtgcgagc tggccccgct cgtcggtggt cttcaggaga tcccccacgg
     8101 ttccccggaa catccggggg atgtagtcga tgtactgggg cttcgtcgcg gtcttcagcc
     8161 gtccctcgga gaccgtgcgg aggtactcga agagctcggt gcccgtgtaa tgggaaagga
     8221 tctccttcca ggggacctcc cggccgggct cccccaggtt cggccggagg ccgccggaga
     8281 ggatctggac cgaggtgctc ttcccgatgc cgttcgggcc gaggaggccg gtcaccttcc
     8341 cccggaccgg aacggggaga ccatagaggg caaagccgtt caccccgtac cggtgggtcg
     8401 gatgggggag ctcttcaggg aggctcacga tgtcgatggc cgagaagggg cacttcttga
     8461 tgcagatccc gcagcccacg cagagctcct cggagacgac ggccgttccc gcctccccga
     8521 tgaccacggt ctcgtcgcca gtccggaccc gggggcagta gaggatgcac tcggtgccgc
     8581 acttcttcgg gtggcatcgg tcccggtgga cgacagcgat cctcatggtc cttcagccgg
     8641 tgggggcgga gagaaggatc gtccaggtga tgaaccagag ggaaaaggtc atgaatccct
     8701 ggtagagcca gtccttcgcg gtaaggccga ggtagtccat tcggagggcg atgaagaggt
     8761 atttctggaa aacgatgccc gcgaggagga agaggatccc gagggcggtt ccggtcgggg
     8821 gcgtttcgat ccggaaggag aggaatccaa cgaggatccc catcccgcat gctatggccg
     8881 tcttcttcag ccggtccatg tgctgggcct gcttctcggc cttcgtgagc ttcttcttcg
     8941 gggttttctc cggttccggt ttcgcctcgg ccgccatccc gccacccagt agtctatttt
     9001 tttagcgctg aagccatatg tagcttggta taccatggcc acggcgcagg gaatgagcgg
     9061 ggtggatctc tcggccgtgg ccgcggagct cctccccctc ctccccctct gggtcgggaa
     9121 gatctaccag tacggtccga aaaccctcgc catccggctc cagggggagg accgggcacg
     9181 ccaccccctc gttatcgagt cagggaggag ggtccacctg gtgaggaccc tcccggaggc
     9241 ccccgagcgg cccgggggct tcgccatgct cctccggaag cacctcgagg gggggagagt
     9301 cctcgggatc gcccagcacg gcctccagag gatcctcacc ctcaccgtcg gaaaaaaggc
     9361 aggcagcctc cggctcgtca tcgagctctt cgacgagggg aacgtcatcc tcgtcgatga
     9421 agggggaaag atcgtgaagc ccctgtggca ccaccggttc cgggagaggg aggtggtccc
     9481 gggcgcggac tacgaattcc cgggaaacga ctgcactgac ctctctcccg ggtccttcgg
     9541 agagctcctc aggtcctcgg atcgggagat cgtgaaggcc ctcgccgtcg actgcatgct
     9601 cgggggccgt tatgcggagg aggtctgcgc catggcgggg atcggaaagg atacccctgc
     9661 aggatccgtg gacccggtag cggttcatgc cgcattcatg gcgctcctcg aacgggcaaa
     9721 gaccggcagg gacccggcca ttaccccctc cggatgctgg cccttccccc tctccgggga
     9781 acctgtaacc tcccggttcc cgagttacga ggaggccctg gaggcctact acggtggccc
     9841 tgccccctcc caggcgaagg agagccgggt ccgggcgaaa cgggaggaaa atatcaggaa
     9901 ccggcaggag tcgagcctcc ggaagttcga ggacgagatc cggcgacggg aaggagccat
     9961 cgaacggatc tacgagaact attccctcgt cgaggatgtg atccgggccc tctcgcaggc
    10021 cgagaagaag acctcctggg aggagatcga aaagactctc agggcgagca ccctcccgtc
    10081 cgcgaaggcc gtcgtcaagt ttcacccgga gtcggcctcg gtgaccctgg acctggggga
    10141 gaaggtgaag atctcgatca gggaatcggt gcgggagaac atcgggcgcc tgcacgagga
    10201 gatcaagaag ttcaggaaga agcgggaggg ggccctcgcc gccctggccc ggcccctccc
    10261 cgccccgccg gagaagaggg agagggccgc cgaagggaag aggcggtggt tcaaccggtt
    10321 ccggtggttc gagacctccg acggggtcct cgtcgtcggg ggaagggacg ccggccagaa
    10381 cgaggagctc gtccggaagt acatggccgg gagtgacacc ttcgtccacg ccgatgtaca
    10441 cggagcgccg gtgctcatcg taaagggagc cacggagcac atggatgagg tggcccagtt
    10501 cgccgcgtcc tactcgggag cctggaagag cgggcacgcc acggcggatg tatactcggt
    10561 ccggcccgac caggtgtcga aagcggcacc ctccggggaa tacctctcca ccggcggctt
    10621 caccatccgg ggcgagcgca cctggttccg ggacgtgccc ctcgccgtgt ccatcggcct
    10681 tgccgaaggg gattcccccc gggtcatcgg cggcccgccc ggggccgtcg tgaaaaaggc
    10741 gacccatcgc gtcaccctcc ggcccggggt cttcgagcca aacgacatcg caaagaaggt
    10801 cgtccgggct ctccgggaga tgatccccga ggccgagcgg ggaggccttc ggagggttct
    10861 ctccaccgag tcggtcgcgg cctttgtccc gccgggcgga tcggaggtgg ttggggaatg
    10921 aaggccgagt tcctcgagat gaaaggttcc cacggggaga tccggctctt ccccgagagc
    10981 gtcgacgacc tctggcacct ctcccacctg atccgccccg gcgacctcgt ctttgccacg
    11041 accttcaggt cggtggagac ggtccaggac aagatccggc ccgagaagcc cgagaagaaa
    11101 ccggtccgcc tggggatccg ggtcgaggga gtcgccttcc acccgtatgc aaaccggctc
    11161 cggatcagcg gcgtcatcca gcacggtgtt gacgaggggt cgcaccacac catcaacctg
    11221 gatgcaggca ccgagatctc ggtcatcagg acctggcggc aggtggacct cgaacggatc
    11281 gaccgggccg tgaaggcaac ctcggcggga gcggtccaca tcctcgccgt cgaggaaggc
    11341 gaggccgagc tctaccggat ccgccagtac ggccccgagc tcgtctcgac ggtcatccag
    11401 ggaagcagca agggtgccga cacgggggcg aggggcatcc tcttcgagcg cgtggccaca
    11461 ctcctcgccg aggtcacggg gacggtcgtc atcgcggggc cggggttcgt gaaggacgag
    11521 gcggcgaagt acctgaagga gaaaatcccg gacctccggg atcggtgcat cacggtggag
    11581 acccggcgga gcgggagggg ggcggtcacc gaggccctcg ggaaaggggt cctcgagagg
    11641 atcacggggg acctccagct gaagcgggag gcggagctcc tcgacctgtt cctcgagcgg
    11701 atggggaagg gtgagccggt cgcctacgga atgaccgagg tggaggaggc cgtctcggca
    11761 ggtgcggcag aacatgtcct cgtcatcgac gagatggtcc ggcagaggca tgtcgccgat
    11821 ctcctggaac gggcggagcg gtcccgggca agggtgacgg tcttctcctc cctcttcgac
    11881 cccgggcagc agctggtcgc cctcgggggc atcgcggcga tactcaggta ccggatgaag
    11941 taaatcagga gcctggagta cccgtatata cttccccggg aaatcctccc gggaaaggga
    12001 gatcgatggt gtgatgggaa ccgattccat agtcttccac gggactctcc ggcagctcct
    12061 ggagaaagtt gagacaggag aaatggtgcc gaagctccag gcctcccatg cccccatgtg
    12121 caagttcttc accgcctact atgtcatcag cggcatccgg cacgtcgtcc ccctggtcca
    12181 tggtcccacc gggtgccccc tgtacgtctc cgactttgtc cggacgaggg agtgctgcga
    12241 gatccggggg gttcccctgg aacccaccgc ctgcacctcc ctcgacgaaa gccatgtcat
    12301 ctacgggggc gaggggaagc tcctggaggc ggtgaaggag gcgtacgcca agtacaatcc
    12361 ggacctcatc gtcatcctct cctgctgctg ctccgggatc atcggcgatg acgtcgagag
    12421 catcgcccgc gaggccgaga agctcgtcgg ctgccgggtg ctcgcgctga gaagcgaggg
    12481 gttcgggggc gatttccgct cggggtacga ggatgccttc aagctcatca tggaactcat
    12541 ggagcccccg agggaaaaac gaaagaggac cgtgaacatc ctcggtgccc gctgggggcc
    12601 caccaccacc gagttcgaca tggacctcga tgaggtggag cggattctcc acgaggtcgg
    12661 gatacaggtc aacgccgtca tcgcaggggg ctgcacggtc gaagaactcc ggagggcccg
    12721 ggaggtggag ctgaacgcct cgtggtgtta tgactggggc cagaagctcg gcgacctcat
    12781 ggaggagcgg ttcgggatcc cgtactcgaa gaccggccag ccctatggcc tcgaggcgac
    12841 gaaggagtgg ctcctcggca tcgcaaagcc cctcgggatg gaggagaagg ccctcgaggt
    12901 catcgagcgg gaggcccggg aagtggaacc ggacctcgaa tacctgaaac aggccctgga
    12961 gggaaagaca gccatcatcg agatcacgga gtttcccggg ccgatccggg ccctctccct
    13021 cgcccggatg gcaggggagt tcggcgccca ccccgtcgtc atcaacatcc acccctacac
    13081 catcagggag aggatgccct ccatcaaatt cctcctcgag acgggtgtca accccgagat
    13141 catcctcacg aagggtctct tccgcctggg gtccttccgc tcgtcgaagg agaccgagga
    13201 tgagctcgag gagatcgtcc ggggatacga tgatcccatc ttcttcggga acgccgcccg
    13261 gtacccgccg tgcccggtgg tgaacctgac cctgatgaac ccgaccttcc agccccagta
    13321 cggcttccgc gggatccgga acatcgccgc cctcgtcagg caggccctcg agaaaaagga
    13381 cctccctcgc tcccggctgc tcaggggaat gctctatggg acccggcgat aggatgtccc
    13441 ctgcggcaca ggatgagggg accggggaga agtgcaccga tccttacctc cggtgtgcct
    13501 tcaacggggc ggcccagacc gccctcgggg tctccggttc ggccctcctc gcccattcgc
    13561 cgcaggggtg cgagtacctg gtgaacaatg ccttcgcctg gcaggagtgc gactacatgg
    13621 agaccatcac cctctgcacc aagctctgcg tggacgagat cgtgcacggg ggcgaggatc
    13681 tcctcgcccg caccatccgg gaggcgaagg gcctccccat ctcggccctc ttcgtggcga
    13741 gcgcctgcgg accggagatc gtcggcgatg acatcgtcgc ggtctgcgag gccgttcagc
    13801 cggaggtgaa gttcaggctc atccccatcg aatccgccgg gttcaggggc tcgcagtacg
    13861 acggcatcga catcgccctc gatgcgattc tctcgcagct cgtgagaagg gatgccgaaa
    13921 aggtcccggg atcggtctgc ctcatcgccc cccatgccaa tgccaaccct acctggccgg
    13981 ccgacctcca gtgggtgaaa cagactctct cccggttggg agtccccgtc atcgccaccc
    14041 tcacccaccg gacccccctc gccgagatcg agagggcctc gtcggcggag acctccctcc
    14101 ttctctccca tgacgcggga cagaaggccg cggaccggct cgcatccgag tacaggatcg
    14161 agcaggtctg cagggggatc ccccttccca tcgggatgac gaacacggcc cgctggctcg
    14221 ccgaactcgg gaggcggttc gaccgtgtga aagaggctga gcagatggtc gccgagggcg
    14281 agaggatggt gatgacgacc tgccggcgca agtggcccat ggcccgcttc ttcttccgca
    14341 ccccggcggc catcgtggcc gacgccacgg tcgggatccc cctcctccgg ttcgtcaccg
    14401 aggacatgga gatgacaccg gatctcatcg ccctctacac cgggcatcgg caggtccggg
    14461 aactcctgga gggagaactc gcggatctcg ggctcaagcc gaaggtcgtg tacgggacgg
    14521 acgtgtataa gacgagaagg ggaatcggcg aggtgaggcc gagggtggtc ttcgggtcca
    14581 ccatcgagcg gcacgcgtcc gagggactcg gggtggcata tgtcgtcgag ctcgtgagga
    14641 tgatgcgcca gttccggttc atcgaccggg aatacttcgg ctataccggg atcctgaacc
    14701 tcttcgaggc cgtccagaac gagtggatca tgcggtggag atcgaaggag aagaggtacg
    14761 aggcaaagtg gtgatgaaga aatgagacag atcgcgatct acgggaaggg gggcatcggg
    14821 aagagcacca tcgcctcgaa cgtctcggcg gcgctcgcgg agatgggcct ctcggtcatg
    14881 caggtcggat gcgacccgaa gagggattcc acgaggatcc tcgccggcgg ggacctcatc
    14941 cccgccgtcc tcgaaacgta ccgggagcag ctccgggtgg gaagggacga gtacgcgatg
    15001 aagctcgata ccatcgtccg gaaaaccccc caggggatct acctcgtcga ggcgggaggg
    15061 ccggagcccg gtgttggctg
//
LOCUS       DATGDI010000051         3966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_237556, whole genome shotgun sequence.
ACCESSION   DATGDI010000051 DATGDI010000000
VERSION     DATGDI010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237556"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..340
                     /locus_tag="VKO45_01935"
     CDS             128..340
                     /locus_tag="VKO45_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04665.1"
                     /translation="MPDAHPLESSLDRLAIIGAVIAVASLWSTLYNPWKDLASSLGLS
                     VAWYVFIFLGPLVIVLILSLWWINTH"
     gene            400..1371
                     /locus_tag="VKO45_01940"
     CDS             400..1371
                     /locus_tag="VKO45_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA04666.1"
                     /translation="MPAPAIEVMNLSKRYGEIQALDGVSFSVPEGEIFGYLGPNGAGK
                     TTTIRILTGITLPTGGTARIFGHDIIAETIESRRHMGIVHETSNVYDDLTAWQNLIFT
                     AELYHVEKAGRDEWARQLLETFDLLARKDDLTHGFSKGMKRRLTLAMGLMNHPRLLFL
                     DEPTSGLDVASNLIIRRVIRELAGEGVTVFLTTHNIEEANLTCDRVAIIDHGRIAAID
                     SPERLKKTIQSVQSVEVGFDRQVPEIRDGLSNIPTVNEVRKEGDKIRFFTDDPSAVIP
                     AIMAYAEREHLRVLSVNTYGPSLEDVFIRLTGLDAGNTGNDRRPLWK"
     gene            1362..2165
                     /locus_tag="VKO45_01945"
     CDS             1362..2165
                     /locus_tag="VKO45_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA04667.1"
                     /translation="MEMKAMLRQIPLDLSAAWAIARKDMIIYYLRPNIIVSGILFPLF
                     MFLAFAIGTVGGGAPAAQAGGASEIAQLGALIPGLLAITVLFSASSIEPVSIPIERRM
                     KTFDRLISAPISLQALVLGESISGTLYSVAIALIPLVIGVLFFGNRILSPVVLVVALF
                     LSSLCFSSMGTLVASYPTENVGEVMSILNLIRFPLIFLSGVFIPLSALPLWGQVVAFF
                     SPLTYTSDLIRFGYAGASHVNPFLDILALAVYVIAFQVFGNYLYRKFNE"
     gene            2230..2829
                     /locus_tag="VKO45_01950"
     CDS             2230..2829
                     /locus_tag="VKO45_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HMA04668.1"
                     /translation="MEFKDIVMQRYATKKFDGRRVPEGKMNELYELIRFAPSALNVQP
                     WKIKVITDQKMKEQLRPATYDQEQVTTCSHLLVFCANTDFEGLLGKLERLMKQAKVPD
                     AELKMVMEMARGYFLANPPEVRLAMAQHHVFLALGNALNGAKSLGLDSCPMGGFDSKE
                     YSRILALPPHLVPTMLCPVGYAADIPEQKVRFARGEIFF"
     gene            2987..>3966
                     /locus_tag="VKO45_01955"
     CDS             2987..>3966
                     /locus_tag="VKO45_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HMA04669.1"
                     /translation="MTFETTKPARAPGFPGTGAGNVSSLRTVSRILRIEGLLPFTGCA
                     VLLGFVVSLWEVGFRAADWGLFLIAVAAAILVHVDAHLWNDIMDLEVDRREKSGETGR
                     DRPLVLGWASVGDYKVLSALITVLVVILAAQMTMQRMFIPFLFVIGFFFDYGYNHPRF
                     ALAYGPFTEWYIFPWLVVGVTVTVVYAATGIFSLLALILSLLHGLTVTCFVVSMMRRD
                     VNSDRLGGKITTSVQHPEIPHATIYGIVTLLASVLMLYPLSIVLGNMGLAYMLVLTTA
                     LIAAINTVHGARIDQLCTRALYSVFPDFELAANRLMLRQVGASLVHAAAIT"
BASE COUNT          692 a         1338 c         1103 g          833 t
ORIGIN      
        1 gaatcccgcg ataccgagga tcactccgac gatggctccg ccgccatggg cgatgatgtc
       61 aattattttc acacatctct ccttccctgt gaaaaactat aaataccaga cttataaagg
      121 tcttcctatg ccagacgcac atcctctcga gagttcgctt gacagattag ccatcatcgg
      181 cgccgttatt gcggtcgcaa gcctctggag taccttgtat aatccctgga aggacctcgc
      241 atcgtcgctc ggtctttccg tagcctggta cgtgtttatc ttcctcggcc ccctcgtgat
      301 cgtcctgatc ctgtccctgt ggtggatcaa cacccactga atttcgccac cgttccaccc
      361 ctcaaccttt ttctcccggt acctcctcat ctaagctcga tgcctgcccc cgccatcgag
      421 gtgatgaacc tctccaagcg gtacggtgag atccaggccc tcgatggtgt ctccttctcg
      481 gtccccgagg gtgagatctt cgggtacctg gggccgaacg gcgcgggaaa gacgacgacc
      541 atccgcatcc tcaccgggat caccctgccg accgggggca cggcccgcat ctttggacac
      601 gatattatcg cagagaccat cgagtcgagg aggcacatgg ggatcgtcca cgagacctcg
      661 aacgtgtacg acgacctgac cgcctggcag aacctcatct tcaccgccga gctctaccac
      721 gtggagaagg ccgggaggga cgagtgggcg aggcagctcc tggagacctt cgacctcctc
      781 gcgaggaagg acgacctcac tcacggcttc tccaagggca tgaagaggag gctcaccctc
      841 gcgatgggac tcatgaacca cccgaggctc ctcttcctgg acgagcccac ctcgggcctc
      901 gacgtggcgt ccaacctcat catccggagg gtgatccggg aactcgcggg ggagggggtc
      961 accgtcttcc tcaccaccca caacatcgag gaggcgaacc tcacctgcga ccgggtggcc
     1021 atcatcgacc acggaaggat cgcggccatc gactccccgg agcggctgaa gaagaccatc
     1081 cagtcggtcc agtccgtgga ggtcgggttc gaccgccagg tgccggagat cagggacggc
     1141 ctttcgaata tacccacggt caacgaggtg cgcaaggaag gtgacaagat ccggttcttc
     1201 accgacgacc cgtcggcggt tattccggcg atcatggcct acgccgagcg ggaacatctc
     1261 agggttctct cggtgaatac ctacgggccg agcctggagg acgtcttcat caggctgacc
     1321 ggcctcgatg cgggaaacac ggggaacgac aggaggcctt tatggaaatg aaggcgatgc
     1381 tccggcagat ccccctcgac ctctcggccg cctgggccat cgcccggaag gacatgatca
     1441 tctactacct ccggccgaac atcatcgtct cggggatcct ctttcccctc ttcatgttcc
     1501 tcgcttttgc catcgggacg gtcggcgggg gtgcgcccgc tgcccaggcg ggaggagcct
     1561 cggagatcgc acagctcggc gcgctcatcc ccggcctcct cgccatcacg gtcctcttct
     1621 ccgcctcctc catcgagccg gtctccatcc ccatcgagcg gaggatgaag accttcgacc
     1681 ggctcatctc ggcccccatc tccctccagg ccctggtgct cggagaaagc atcagcggga
     1741 ccctctatag cgtcgccatc gcccttatcc ccctcgtcat cggcgtcctt ttcttcggga
     1801 accggatcct ctcgccggtc gtcctcgtcg ttgctctgtt cctctcctcc ctctgcttct
     1861 cctcgatggg aaccctcgtg gcctcgtacc cgaccgagaa cgtcggcgag gtgatgtcaa
     1921 tcttaaacct catccgcttc cccctcatct ttctctcggg ggttttcatc cccctgagcg
     1981 ccctccccct ctggggacag gtggtggcct tcttctcccc cctcacgtac acgagcgacc
     2041 tcatcaggtt cggctatgcc ggtgcgagcc acgtcaaccc cttcctcgac atcctcgccc
     2101 tcgcggtgta cgtcatcgcc ttccaggtct tcgggaacta cctgtaccgg aagttcaacg
     2161 agtgagggac ccccacggaa catttttcat cgtccctgat cattcctcat ctgtattcgg
     2221 aggtccccca tggagttcaa ggatatcgtc atgcagcggt acgcgacgaa gaagttcgat
     2281 ggaagaaggg ttcccgaggg gaagatgaac gagctctacg agctcatccg gttcgctccc
     2341 tcggcgctga acgttcagcc ctggaagatc aaggtcatca ccgaccagaa gatgaaggag
     2401 cagctgagac cggcgaccta tgatcaggag caggtcacca cctgctccca ccttctcgtt
     2461 ttctgcgcaa acaccgattt cgagggtctc ctcgggaagc tcgagcggct gatgaaacag
     2521 gcgaaggttc ccgacgcgga actgaaaatg gtcatggaga tggcgcgggg ctatttcctc
     2581 gcgaatcccc ccgaggtgcg gctcgccatg gcccagcacc atgtcttcct cgcgctgggg
     2641 aatgccctga acggggcaaa gtccctcggc ctcgactcct gccccatggg cgggttcgat
     2701 tcgaaggagt actcgcgcat cctcgcgctc ccgccccacc tcgttcccac catgctctgc
     2761 ccggtcggct atgccgcgga cataccggaa cagaaggtcc ggttcgcgag aggggagatc
     2821 ttcttttagg ccggtccggg agcctcctct ctgggaagag aggaaacccc gaacccgttt
     2881 tccatacagg agaattatta tatgcctgcc cgcgatgtat ggcattgcga acgggaagtt
     2941 ccgtgatttt tacggtcctg cctcgttgac cgtgcggcag gtgtccatga ccttcgaaac
     3001 gacgaagccg gcccgggcgc cgggctttcc cggtacaggg gcggggaacg tttcttctct
     3061 ccgcaccgtc tcccgcatcc tgcggatcga gggactgctc cctttcaccg ggtgtgcagt
     3121 cctcctcggg ttcgtggtct ccctgtggga ggtcggtttc cgggccgccg actggggcct
     3181 ctttctcatc gcggttgccg cggccattct cgtccacgtc gatgcccacc tctggaacga
     3241 catcatggac ctggaggtcg accggcggga gaagagcggg gaaacgggcc gggatcgccc
     3301 cctcgtcctg gggtgggcca gcgtcgggga ctataaggtc ttatcggcgc tgatcaccgt
     3361 cctcgtggtg atcctcgccg cccagatgac gatgcagcgc atgttcatcc cgttcctgtt
     3421 cgtcatcggc tttttcttcg attacgggta caatcatccc cgcttcgccc tcgcgtacgg
     3481 gccctttacg gagtggtaca tcttcccctg gctggtcgtc ggggtaacgg tgacggttgt
     3541 ctacgccgcg accggcatct tttccctcct cgccctgatc ctgtccctcc tgcacggtct
     3601 cacggtgacc tgtttcgtgg tatccatgat gcgccgcgac gtgaactcgg accggctcgg
     3661 ggggaaaatt accacctccg tgcagcatcc ggagataccc cacgcgacca tctatggcat
     3721 cgtcaccctc ctcgcgtctg tgctgatgct ctaccctctg tccatcgtcc tggggaacat
     3781 ggggctcgcc tacatgctcg tccttaccac ggcccttatc gcggcgatca acacggtcca
     3841 cggggcaagg atcgaccagc tctgcacccg tgccctctac tccgtcttcc ccgacttcga
     3901 gctcgcggcg aaccggctca tgctgcggca ggtcggggct tcccttgtcc acgcggcggc
     3961 catcac
//
LOCUS       DATGDI010000052        19159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_353852, whole genome shotgun sequence.
ACCESSION   DATGDI010000052 DATGDI010000000
VERSION     DATGDI010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 19159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19159
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353852"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..851)
                     /locus_tag="VKO45_01960"
     CDS             complement(66..851)
                     /locus_tag="VKO45_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMA04670.1"
                     /translation="MKGPCRIAISGKGGTGKTTLSALLVQALIGMEETPVLAVDADPN
                     ANLNEALGLPLGETLGSMREDAFTKSIPPGMSRTGYIEYRFQQVLVESEGVDLLAMGR
                     PEGTGCYCFANRLLSEAMDLLVRHYRFVVMDMEAGMEHLSRGTIGIPDILLVVTDPGA
                     RGMRTAVRIHDLAVTLGIPKERIFFVINRLGTAAGVAGGKGSGTGSEPVLDPSRILAS
                     IPDDPEVERADLEARPIVSVPEGGPARRAVGDLAARVRSLCTG"
     gene            complement(848..1891)
                     /locus_tag="VKO45_01965"
     CDS             complement(848..1891)
                     /locus_tag="VKO45_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04671.1"
                     /translation="MKALSRPIAPPGSLATGVGALPHRDPRAAVDEVLRIFPRFPYVP
                     TLPNRGFLESIVFSDSAMLPGRMIRDGRLLVDASRDITPEMEQIYADYLEKNTAPYAT
                     TPEYGSGFFEMTSRDLEEAFLLKCQVTGPVTFGMQVVDASKRPIYYDPQFADILGKLI
                     ALRARWCEEEIRRRTGLSHTLVILNEPYLASLGSSVVPVDRDSVAAGWRDISALVEGG
                     LGVHCCTNTDWGFLISLGPSVISLDAYGNAREFLLFMDEVSGFMEAGGVVAWGIIPSD
                     PRVFSRETVDSLTARYLGIRRAVLEHVDKEVFDGKSLITPSCGIQLGTEAEAVAIMEA
                     AAAVSARVRGMAP"
     gene            complement(1888..3789)
                     /locus_tag="VKO45_01970"
     CDS             complement(1888..3789)
                     /locus_tag="VKO45_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HMA04672.1"
                     /translation="MPTVTFLPSYRKVVVDPGTTILDAARQAGFQMNVVCGGQGKCGK
                     CVVFVKAGDASFDREKFGRFFTPEELDGGACLSCQSEVTGDLQVLIPESTLIQEQKIL
                     IEALGMETAFSPSVWKYALELQPPTLDDPSPDLTRLLWGIQKKGGPVVEKIYAPLELL
                     RLIPRILRESGWRVTGTVARVPGGYRLINVEKGDTTERLYGAAVDLGTTTIVVYLRSL
                     VDGKVVGVASNYNRQISCGEDILSRVNYARKGGLPRLQALAAESINLALKGAANAAGI
                     DIDDIYEVVVSGNTIMTHILMGMDPAYMIEEPYVPVVRRYLTASAQRLGLVVNGNAGL
                     FIFPAVSDFIGGDIVADILACGMGESEEVSLMIDIGTNFEVVLGNREWMFACAGAAGP
                     ALEGGEVLFGMRANPGAIERITLDLETLDPKYETINQVKPRGICGSGLIDLLAELLRA
                     CVIDRTGRIDTGIKNERVRMGARYPEFVVAWKEESGGEKDIVITENDVKSLIMSKASI
                     LAACFILMNEAGIGKDAVKNIYFSGAFGNYLNKENAILVGLVPEVPVERIKNIGNGAV
                     EGANIALLNRKMRKRLDEIARTVAYIELNAEPAFMDEYTRASFLPHTDLSLFPGVQRI
                     LDSCRVRRG"
     gene            complement(3935..5287)
                     /gene="acsC"
                     /locus_tag="VKO45_01975"
     CDS             complement(3935..5287)
                     /gene="acsC"
                     /locus_tag="VKO45_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HMA04673.1"
                     /translation="MALKALEIYKMLPKKNCKECGYPTCLTFAMKLAAGKADADSCPY
                     LDESTKQLLRGATRPPIQLVKLGVGERQVPVGDEIVLFRHEKSFYHPPGIFFSVSDLW
                     DGDRIRAAAERVMGEVFPRVGEYLRFNGLAVRNESGEADAFGRAVAAVEEVAEFPEVL
                     VSGSPEALAAGLAQCGNYRPVIHAATRENHREVAALAKRFGCPLVVRDPDPAGLADLA
                     KACTGLGAPNILLDPAPANLGEFLARSTTIRKKALDRSDPDLGHPVYLDATTMVPEEA
                     GIVTGILKYAGVIVTSVLPPGTARMALTLRQNIYTDPQKPIQMTPGLYRVGEPGPSSP
                     VLASVNFSLTYFTLLGYLEASRIPCHLLIVDTEGFSVLTAVAAGKLSERLIKDSLDRF
                     QVADQVKHRILVIPGYAAPLSGRIEETTGWKVLVGPRDAADIGEFLEREWVAKGQAPP
                     "
     gene            complement(5277..6248)
                     /locus_tag="VKO45_01980"
     CDS             complement(5277..6248)
                     /locus_tag="VKO45_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HMA04674.1"
                     /translation="MGDPLPPESAAGIDVVTLGATEKDGGTRSVSYRIGGWSSYPFLG
                     PRPAGVQSPLVALEICDDPRTWPAVVTRDLGDLARDPAAWAGEAQRRFSADLVRLVLT
                     STRDRSFSDFPSVGRTVEAVLSATSLPLIIEGSSEPDLDREVFQRCGEAGAGERLLLG
                     TAEADRYRSVAAAALAYGHAVVAQSPIDINLAKQLNILLREIGVPHDRIVIDPYTGAL
                     GYGFEYSYSVMERIRDAALKGDRDLAMPMISQAPDSLVVKEVREGAPEWQREIAVRWE
                     FGFALSAAIAGADIVSVRHPETVRLLKEAFRSLLAGGTTQGGGAGGA"
     gene            complement(6260..8290)
                     /gene="acsB"
                     /locus_tag="VKO45_01985"
     CDS             complement(6260..8290)
                     /gene="acsB"
                     /locus_tag="VKO45_01985"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HMA04675.1"
                     /translation="MSEIDAGVGQGTGQLKKAMAAISGTLGYAETAYHLPISYALTGI
                     DVKDGPSAREAYAKSGENPLVACECLLAARAAASGSEGGPFTGFIPDTVLRKLGYSLV
                     DGSILGMALLAGSPASADEAAGMARELQEKYMLTFLAGDIIRTLTTAGVKTGLDYRLV
                     PLGSRPLHGVHFADIVARVAMMFGGVVPGDAGRLIAYAAERAKVFVIVFPGLSDEEIA
                     FADTLRALSVPIITLGGYEGGSWIHATADEAVRRGMEEKDIRVKVTAIPIPMGCSPAF
                     EGKSIRKEEMYVEFGGGRSPAFELLRMREPGEVVDGRVTVVGPEIGEMKQGSAIPLGI
                     LVEVTGKRMRKEFEPVLERRIHNFVNYGEGSWHVAQRNLIWVRLSKEAVAKGVRVEHL
                     GKLIGAKFKMDFPELLDAVQVTLITDGKKVVEMMEEAERVYEERDRRVLGMKDDQVDI
                     YYSCTLCQTFAPNHVCVITPQRPALCGAISWLDGRTAYEISPSGANQPIKKGRLIDAQ
                     RGEFEGVNEFVKKASHGEVDRCSLYSLMEFPMTCCGCFECIAVVLPEVNGVMVVNREF
                     TGDTPCGMSFSTLAGTIGGGAQTPGFAGISKAYILSERFLQAEGGIARLVWMPSMLKE
                     ELEPRLRKRLGDIGSPDLIGKIATENEAKTLEELQSFLEKVGHPALSLKPLV"
     gene            8645..9256
                     /locus_tag="VKO45_01990"
     CDS             8645..9256
                     /locus_tag="VKO45_01990"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HMA04676.1"
                     /translation="MVPAKIFFTKGVGIHKDKLASFELALRKAGIEKFNLVHVSSILP
                     PGCRQVSADDGLKLLQPGQIVFVVMARNETNEPNRLVSAAIGLARPRAQDDYGYLSEH
                     HTFGETDEKSGEYAEDLAATMLATTLGIEFDPDLAWQEREQIFKASGKIFKTTHICQS
                     AQGEKTGKWTTVISVAVFVEDNNNQTKLPLGPPPPQEPDQKTK"
     gene            complement(9241..10413)
                     /locus_tag="VKO45_01995"
     CDS             complement(9241..10413)
                     /locus_tag="VKO45_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HMA04677.1"
                     /translation="MDNSGKAEPSYRKSLGLVELVSLGVGGTIGSGIFVVPGIAAGIA
                     GPSSLFAWIFVALSAVCVALSLAWIQSRSPGGTPFVAIFEPVFGPRASGVLATVYLVS
                     SVFGVATIAAGLGQYFGFFSVPHILAAEIFVIMAFLGINLVGIALSGRTENILTAAKI
                     VPIVLIAAALLPFIRPENLVPARAASLPDILKVVIIVYWPFTGFEISAIPVEETRDPR
                     TISRALLLVMLLVCTIYLMLNVALIGAVGSADLAASPAPVATAAGLVFSGAAPLVAII
                     GIITMFSALNAYIIGTSRVLQNMAGRYRLPRLAELTPRGTPKTALVISCILSGGLLFF
                     SNSFNLLASVAVLTTLVPYIAICLVSLKLAREPRVRALGIVGAATTAAILVLYFVF"
     gene            10599..10928
                     /locus_tag="VKO45_02000"
     CDS             10599..10928
                     /locus_tag="VKO45_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04678.1"
                     /translation="MNVKETLETFLEEGKDWERKQTSIRGVSILRMPRTTTRPPTLAM
                     EINPVDDRGAQMKRKGVMIRSPKELDSLRGILSDPKVVNLMEALASVVPPVPAAGKEP
                     AAGVIEV"
     gene            complement(11020..12135)
                     /locus_tag="VKO45_02005"
     CDS             complement(11020..12135)
                     /locus_tag="VKO45_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HMA04679.1"
                     /translation="MLLDTDLHIHSRFSIASSRSMVPSSILAGCLRKGLAAVGSGDAL
                     HGKWREMWGEAGREDGVLVIPSAEVEDAHRVHHLILAGSFDTFGDLAGRFSPSAPSIA
                     SMGRPHLRLAGEEIAREVHDAGGLIGPAHAFTPWTSLYASFDHVRDCYGDERPDFLEL
                     GLSADSSYGAGISELRGIPFLSNSDAHSPSPAKMGREFNRIEVPHRTVGEVLESIRKG
                     RIAMNAGFFPEEGKYNRTACTRCFREFSLEEAAALSWRCPDDGGLIKKGVSDRARELS
                     DMPPEPRPPYLHLIPLAEILQRVLGTSSPNTKKVAALYDAFLGAFGTEIRVLVDVPVE
                     ELQELHPVAGDAIARLRTGRVRLFPGGGGRYGRFSFE"
     gene            complement(12141..12884)
                     /locus_tag="VKO45_02010"
     CDS             complement(12141..12884)
                     /locus_tag="VKO45_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HMA04680.1"
                     /translation="MDPIEVRWLSEEPGTAEILIEGLPGIGHVGKLVAEYLIDELRAE
                     RIAEIYSLHFPPQVIIGPDGVARLARNEVFLHEGDKGIAFLVGDYQSVSAEGHYLLAE
                     AYLDIAERLGVRRIFTLGGYGVGHLVSEPRVLSAVNRGELRAGVEAAGAEFTCDEPPG
                     GIIGASGLLLGLSLRRGMDAVCLMGETSGYLVDPRAASGLLTVLSALTGVEVDRSRLE
                     TRAAEMEQVIAKLTEGEKPGDDEALRYFG"
     gene            complement(12872..13033)
                     /locus_tag="VKO45_02015"
     CDS             complement(12872..13033)
                     /locus_tag="VKO45_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030515 - snoRNA binding [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleolar RNA-binding Nop10p family protein"
                     /protein_id="HMA04681.1"
                     /translation="MSGRIRRCPIDSTYTLSPLCPSCGRPTATAHPARFSPDDRRGRY
                     RRLSRGWTR"
     gene            complement(13038..13817)
                     /locus_tag="VKO45_02020"
     CDS             complement(13038..13817)
                     /locus_tag="VKO45_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HMA04682.1"
                     /translation="MAERDWPEEGELVVCTVEAVKDFVAFVRLDEYGGRQGLIPISEI
                     AKGWVKYIRDHVREGQKVVCKVLHVDRSRGHIDLSLKDVNEHQRREKIQEWKNEQKAH
                     KWIGFVSEESKVPAPAIEDAIRKDYTHLYRAFEDVVVEGEKVFEKLAIPPPARAAFLK
                     IAGENVRKPRVTIAGYLHLTSTKPDGVSVIRKALKSALPKIENVDIELLYVGAPIYRI
                     KVTAPDYKQAEKAIERSATAAIRVMEKSGDTGEFVRKQKAG"
     gene            complement(13825..14013)
                     /locus_tag="VKO45_02025"
     CDS             complement(13825..14013)
                     /locus_tag="VKO45_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HMA04683.1"
                     /translation="MVRLNRENRSRFYRVKCPDCENEQVIFGKASTVVDCIVCGKVLA
                     EPAGGKAHITAEILSEIE"
     gene            complement(14018..14296)
                     /locus_tag="VKO45_02030"
     CDS             complement(14018..14296)
                     /locus_tag="VKO45_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HMA04684.1"
                     /translation="MKMPVKFNTYCPFCRKHELHEVERVKKGKSRGLHWIDRQKARRG
                     KVGNMGKFSKVPGGDKPTKKINVRYRCTKCKKAHLRPGFRLSKFELTE"
     gene            complement(14444..15073)
                     /locus_tag="VKO45_02035"
     CDS             complement(14444..15073)
                     /locus_tag="VKO45_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04685.1"
                     /translation="MALDDLRIVILNERESGRLSEVPPTLYDHIRGHLASLQEQVYGC
                     SDPLTDQAQTLIEEVKAVREIARDIFRIRSRKILSLALVQVEAGTAERDEVKKMLPAE
                     REMFDGIIRELEKGKAALSGEIPRGGVPGVTPPAGVSPEAAGIPRPAMPPEEAMVLVR
                     ILSDMEPFMGVDGRVYNLKAEDLVTLPSKNAGVLIDRNIAMDVMKAPEG"
     gene            complement(15060..16208)
                     /locus_tag="VKO45_02040"
     CDS             complement(15060..16208)
                     /locus_tag="VKO45_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit PriS"
                     /protein_id="HMA04686.1"
                     /translation="MRPATTEFLRQRFTEYYRRERIMAPPSVGEREFGFILFDPDSPE
                     VRMRRHMGFGTAGELGEYLRSMVPAHAFYSSAYYTAPGAGTMAEKGWTGADLIFDLDA
                     DHLMRGPYRAMLDRVKLETQKLLAMLTGELGFSERIIHLVFSGGRGYHVHIRDIGVRG
                     WGSQERRELIDYVCGIGIDPGALLSGVRGGTPGWSERYISTISEYLLWLKGLGREKGV
                     EHLMEFPGIGRERAPRFFDAIDTYLAGLGRGDSGPLAENQVFAAISAGGKFQELLRGR
                     AALADEPVTTDTRRLIRMPTSLHGGSGFRVTPLALGDFDSFDPLRDAVVFSDRPVKVE
                     SGANISMEILGNRYQVDKGTSAVPEALAVFLCARGLAEIAGEGGHGPR"
     gene            complement(16205..17779)
                     /locus_tag="VKO45_02045"
     CDS             complement(16205..17779)
                     /locus_tag="VKO45_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HMA04687.1"
                     /translation="MDEFALCREIISRLSSHPLSPSRLEHLKIEVCRAHHSPVIPKNS
                     AILAAARPEERERLRELLLLKPTRTLSGVAPVAVMTSPAPCPHGKCLPCPGGPDHPFG
                     TPQSYTGEEPAALRAREHGYDPYRQVRARLDQFRALGHHVDKAELIVMGGTITARPPE
                     YQQWFVSECIRAMNESGGVTGETGEPFERIAAANETSAVRCVAVTFETRPDYCREEHV
                     KRMLSLGVTKVELGVQHVDDRILDLNRRGCSLADAVEANRLLRDSGFKVGFHVMPNLP
                     GASIADDEAMFRTLFSDEDLRPDFLKIYPTLVTPGSGIEDLYRNGGYEPYPEPVLVDL
                     IARAKALLPPYVRLQRVQRDIPAKLITAGSIHGNLRQMARSRLGEMGGACRCIRCREA
                     GRCTPGGTPGFEVLTYPCCGGTEHFISAVAGEALIGYARLRFPGDPGKAVPEEASLLR
                     ELHVYGPLVPLGMDAGSAEWQHRSFGSVLLERAEDLSRKGGFRGITVLPGIGVRPYYR
                     RRGYERRGPYMVKELA"
     gene            complement(17783..18049)
                     /locus_tag="VKO45_02050"
     CDS             complement(17783..18049)
                     /locus_tag="VKO45_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HMA04688.1"
                     /translation="MQKEELLHLHMLLIHVKKYYENLTSDEIFTDRYNALTISPVHIH
                     KNKILHKKAILTLGDEIVSHIRARRPAMVEMSPEAATGRVAAEQ"
     gene            18102..18971
                     /locus_tag="VKO45_02055"
     CDS             18102..18971
                     /locus_tag="VKO45_02055"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HMA04689.1"
                     /translation="MFILQEERAVGVLLGLAVGDALGAPLEGLPPPSVPVRDLPAGGS
                     YTDDTLQAMAVARSLVVSRGFSPEDCMARLLDGYRRYPEFYGPTSSGVFALVLEGVPA
                     RDAALQVHRTLGGSRSNGSVMRGPPLGIFTASPVLEEMSLACSRLTHCDPVAGACSAF
                     VNRMIASLAREASRGEAFRLACSTCRSGEVLSMLGEYRKNPRDPSLDALLATHCALSV
                     FMESASFAEAVLGAVNMGGDADTIGAITGALAGAAWGAGAVPPLWRVNLKDHDRIIEL
                     AGGLYRVAVEKGE"
     gene            complement(18991..>19159)
                     /locus_tag="VKO45_02060"
     CDS             complement(18991..>19159)
                     /locus_tag="VKO45_02060"
                     /inference="COORDINATES: protein motif:HMM:NF015631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HMA04690.1"
                     /translation="TFEVSCNKEECKSFELCRPDGVIDGERYLVGEILGNAPDICEKG
                     RALKLVELRPL"
BASE COUNT         3239 a         6283 c         6425 g         3212 t
ORIGIN      
        1 gggccccttc accatcgagg cgggttcaaa ggtcacgaag aaggataccc gggaagggta
       61 ggggctcatc cggtgcagag ggagcggacc cgtgccgcca ggtccccgac agcacggcgg
      121 gcgggacccc cctcggggac ggagacgatg ggccgggcct cgaggtccgc cctctccacc
      181 tcggggtcat cggggatcga cgcgaggatt ctcgacggat ccagaaccgg ttctgatcct
      241 gttcctgatc ctttcccacc cgccaccccc gccgccgtcc ccagcctgtt gattacgaag
      301 aagatcctct cctttggaat acccagcgta accgcaaggt catggatcct caccgccgtc
      361 ctcatccccc gggccccggg atcggtgacc acgaggagga tgtcggggat cccgatggtc
      421 ccccgggaga ggtgctccat ccccgcctcc atgtccatca ccacgaaccg gtagtgacgg
      481 acgaggaggt ccatggcctc ggagaggagc cggttcgcga aacagtagca cccggtcccc
      541 tcgggccttc ccatggcaag gaggtcgacc ccctccgact ccacgaggac ctgctggaac
      601 cggtactcga tgtagccggt ccgggacatc ccgggcggga tggacttcgt gaaggcatcc
      661 tcccgcatgg acccgagggt ctcgccgaga ggaagaccga gggcctcgtt caggttcgcg
      721 ttggggtctg catccacggc gagcaccggg gtctcctcca tgccgatgag ggcctggacg
      781 aggagggcgg agagggtggt cttcccggtc ccgcccttcc ccgagatggc gatccggcag
      841 ggccccttca tggcgccatc ccccgcaccc gggccgagac ggcagccgcg gcctccatga
      901 tggcgacggc ctctgcctcg gtcccgagct ggatcccgca gctcggggtg atgagggatt
      961 ttccgtcgaa gacctccttg tcgacatgct ccaggacggc tctccggatc ccgaggtagc
     1021 gggccgtgag ggagtccacg gtctcccggg agaacacccg cgggtcagag gggatgatcc
     1081 cccaggcgac gaccccgccc gcctccatga atcccgatac ttcgtccatg aagaggagga
     1141 actccctcgc gttcccgtac gcatcgaggg agatcaccga cggcccgagg gagatgagga
     1201 agccccagtc ggtgttcgtg cagcagtgga ccccgaggcc tccctcgacg agggccgaga
     1261 tgtcgcgcca tccggcggcg acggagtccc ggtccacggg gaccaccgag gagccgaggg
     1321 aggcgaggta cggttcgttc aggatcacga gggtgtggga gaggccggtc ctcctccgga
     1381 tctcctcctc gcaccagcgg gcccggaggg cgatgagctt cccgaggatg tccgcgaact
     1441 ggggatcgta gtagatgggc cgtttcgatg cgtccaccac ctgcatcccg aaggtgacgg
     1501 gcccggtgac ctggcacttg agaaggaacg cctcctcgag atcccgggag gtcatctcga
     1561 agaaaccaga gccatactcc ggggtggtgg cgtacggggc ggtgttcttc tccaggtagt
     1621 ctgcgtagat ctgctccatt tcgggggtaa tgtcccggga tgcgtcgacg aggagcctcc
     1681 cgtcccggat catccggccg gggagcatcg ccgagtcgga gaagacgatg ctctccagga
     1741 atccccggtt cgggagggtg gggacatagg gaaaccgggg gaagatccgg agcacctcgt
     1801 cgacggcagc cctcgggtcc cgatggggaa gcgcccccac gcccgtggcg aggctgcccg
     1861 ggggagcgat gggcctggac agggctttca ccccctccgg acccggcagg agtcgaggat
     1921 ccgctgcacg ccggggaaga gggagaggtc ggtgtggggg aggaaggagg cccgggtgta
     1981 ctcgtccatg aaggccggct cggcgttcag ctcgatgtag gccacggtcc gggcgatctc
     2041 gtcgagacgc ttcctcatct tccggttgag gagggcgatg tttgctccct ccaccgcccc
     2101 gttcccgata tttttgatcc tctccaccgg gacctcgggg acgagcccga cgaggatggc
     2161 gttctccttg ttgaggtagt tcccgaaggc cccggagaag tagatgttct tcacggcgtc
     2221 cttcccgatc ccggcctcgt tcatgaggat gaagcaggcc gcgaggatgg aggccttgga
     2281 catgatgagg cttttcacgt cgttctcggt gatgacgatg tccttctccc cgccggactc
     2341 ctccttccat gcgacgacga actccgggta acgggccccc atccggacgc gctcgttttt
     2401 gatgcccgtg tcgatcctgc ccgtccggtc gatgacgcag gcccggagga gctcggcgag
     2461 gaggtcgatg agcccggaac cgcagatccc ccggggcttc acctggttga tggtctcgta
     2521 cttcgggtcg agggtctcga ggtccagggt gatccgctcg atggccccgg ggttcgcccg
     2581 catcccgaag aggacctcgc cgccttcgag ggccgggccg gcggccccgg cgcaggcgaa
     2641 catccactcc cggttcccga ggaccacctc gaagttcgtc ccgatgtcga tcatgaggga
     2701 gacctcctcc gactccccca tcccgcaggc gaggatgtcc gccacgatat ccccgccgat
     2761 gaagtcgctc accgccggga agatgaagag gccggcattc ccgttcacga cgagcccgag
     2821 gcgctgcgcc gaggcggtga ggtaccggcg gaccaccggg acgtagggct cctcgatcat
     2881 gtaggccggg tccatcccca tgaggatgtg ggtcatgatg gtgttccccg agacgaccac
     2941 ctcgtagata tcgtcgatgt caatcccggc ggcgttcgcg gcgcccttca gcgcgagatt
     3001 gatgctctct gcggcaaggg cctggagcct cgggaggccg cccttccggg cgtagttcac
     3061 ccgggagagg atgtcctccc cgcagctgat ctgccggttg tagttcgagg cgacccccac
     3121 caccttcccg tcgacaaggg accggaggta cacgacgatg gtggtggtcc ccaggtccac
     3181 ggcagccccg tagagccgct ccgtggtgtc gcccttctcg acattgatga gccggtagcc
     3241 gcccgggacc cgtgcaaccg ttcccgtcac ccgccagccg ctctcccgga ggatgcgggg
     3301 gatgagccgg aggagctcga ggggggcgta gatcttctcg acgacgggtc cgcccttctt
     3361 ctggatcccc cagaggagcc gggtgaggtc cggcgagggg tcgtcgaggg tcgggggctg
     3421 gagctcgagg gcgtacttcc agaccgaggg ggaaaaggcg gtctccatcc cgagggcctc
     3481 gatgaggatc ttctgctcct ggatgagggt gctctcgggg atgaggacct ggaggtcgcc
     3541 cgtcacctcc gactggcagc tgaggcaggc ccccccgtcg agctcctccg gggtgaagaa
     3601 ccgcccgaac ttctcccggt caaaggaggc atcccccgcc ttcacgaaga cgacgcactt
     3661 cccgcacttg ccctggccgc cgcagaccac gttcatctgg aacccggcct ggcgggcggc
     3721 gtcgaggatg gtggtcccgg gatcgaccac gaccttgcgg taactgggga ggaaggtgac
     3781 cgtcggcatc aggggaccct ccctgcaccg gaccatgcgg gtccgtccgt cttcatggta
     3841 cagatgttag gaaaaagggg ccacatatac atatggtgcc ccccgggcac cggaaccggg
     3901 gcctcccacc gggggctgtc cctgccggga atatttacgg gggggcctga ccctttgcca
     3961 cccactccct ctccaggaac tcgccgatgt cggccgcatc cctcggcccg acgaggactt
     4021 tccagccggt cgtctcctcg atccggcccg agaggggggc cgcgtatccg gggataacca
     4081 ggatccggtg cttcacctgg tccgcgacct ggaaccggtc gagggagtcc ttgatgagcc
     4141 gctcggagag cttccccgcg gcgacggcgg tgagcaccga gaagccctcg gtgtccacga
     4201 tgaggaggtg gcaggggatc cgggaggcct cgaggtaccc gaggagggtg aagtacgtga
     4261 gggagaagtt cacggaggca agtaccgggg aggaggggcc gggctccccg acccggtaaa
     4321 ggcccggggt catctggatg ggcttctggg gatcggtgta gatgttctgc cggagggtga
     4381 gggccatcct cgcggttccc ggggggagga ccgaggtgac gatgaccccg gcatacttga
     4441 gaatgccggt cacgatcccg gcctcctcgg gaaccatggt ggtcgcgtcc aggtataccg
     4501 ggtggccgag atccgggtcg ctccggtcga gggccttttt gcggatcgtc gtggaccggg
     4561 cgagaaattc accgaggttc gcgggggccg ggtcgaggag gatgttcggg gcgccgagcc
     4621 cggtgcaggc cttcgcaaga tccgcaagcc cggccgggtc gggatccctg accacgaggg
     4681 gacagccgaa gcgcttcgcg agggcggcca cctccctgtg gttctcccgg gtcgcggcat
     4741 ggatcaccgg ccgatagttc ccgcattggg cgaggccggc ggcgagggcc tcgggggaac
     4801 cggagacgag gacctcggga aactcggcca cctcctcgac ggcggccacc gcccggccga
     4861 aggcatccgc ctccccgctc tcgttccgaa cagcgagccc gttgaacctg agatactccc
     4921 ccaccctcgg gaagacctct cccatcaccc tctcggccgc ggcccggatg cgatccccgt
     4981 cccagaggtc ggagaccgag aagaagatcc ccggcgggtg gtaaaagctc ttctcgtgcc
     5041 ggaagaggac gatctcgtcc ccgacgggga cctgccgctc gccgacgccg agtttcacga
     5101 gctggatagg gggccgggtc gcgcccctga ggagctgctt tgtcgattcg tccaggtagg
     5161 gacagctgtc ggcatcggcc tttcctgcgg cgagcttcat ggcaaaggtg aggcaggtgg
     5221 ggtacccgca ctccttgcag ttcttcttcg ggagcatctt gtagatctcg agggctttaa
     5281 gcgccacccg cgccaccccc ctgggtcgtc ccgccggcga gaaggctccg gaaggcctcc
     5341 ttgaggagcc tcaccgtctc cgggtgcctc accgagacga tgtcggcccc cgcgatggcg
     5401 gccgagaggg caaacccgaa ctcccacctg accgcgatct cccgctgcca ttccggggcg
     5461 ccctcccgca cctccttcac cacgagggaa tcgggggcct ggctgatcat gggcatcgcg
     5521 aggtccctgt cgcccttcag ggccgcgtcc cggatccgct ccatgaccga gtaggagtac
     5581 tcgaagccgt acccgagggc gcccgtgtac gggtcgatga cgatccggtc gtgggggacg
     5641 ccgatctccc ggaggaggat gttcagctgc ttcgcgaggt tgatatcgat gggggactgg
     5701 gccacgaccg cgtggccgta ggcgagggcg gccgcggcca ctgaccggta ccggtcggcc
     5761 tcggcggtcc cgaggaggag gcgctccccg gccccggcct ccccgcaccg ctggaagacc
     5821 tcccggtcca gatccggctc gctcgatccc tcgatgatga gggggaggga ggtcgccgag
     5881 aggaccgcct ccacggtcct cccgacggaa gggaagtcgg agaaggaccg gtccctcgtc
     5941 gatgtgagga cgaggcgcac caggtcggcc gaaaagcgcc tctgggcctc ccccgcccag
     6001 gccgccggat cccgggccag atccccgaga tcccgggtga cgacggcggg ccaggtccgc
     6061 gggtcgtcgc agatctcgag ggcgacgagg ggggactgca cccccgcggg ccgcgggcca
     6121 aggaaggggt aggaactcca gccgccgatc cggtaggaga ccgaacgggt cccgccgtcc
     6181 ttctccgtcg ccccgagggt gacgacgtcg atcccggctg ccgattcggg gggaagagga
     6241 tcacccatgg gagcctccgt cagacgaggg gtttcaggga gagggccggg tgcccgacct
     6301 tctcgaggaa cgactggagc tcctcgaggg tcttcgcctc attctcggtg gcgatcttcc
     6361 cgatgaggtc cggggacccg atgtcaccaa gtcgcttcct cagccggggt tcgagttcct
     6421 ccttgagcat gctgggcatc cagacgagcc tcgcgatccc gccctcggcc tggaggaacc
     6481 gctcggagag gatgtaggcc ttcgagatac cggcaaagcc cggcgtctgg gcaccacccc
     6541 cgatggtccc ggcgagggtc gagaaggaca tcccgcaggg cgtgtccccc gtgaactccc
     6601 ggttcaccac catgaccccg ttcacctcgg ggaggactac cgcgatgcac tcgaagcagc
     6661 cgcagcatgt catggggaac tccatgaggg agtagaggga gcaccggtcc acctccccgt
     6721 gggaggcctt cttcacaaac tcgttcaccc cctcgaactc cccgcgctgg gcgtcgatga
     6781 gccggccctt ctttatcggc tggttcgccc ccgaggggga gatctcgtac gcggtccgcc
     6841 cgtcgagcca ggagatggcc ccgcagaggg cgggtcgctg gggggtgatg acgcagacgt
     6901 ggttcggggc aaaggtctgg cagagggtgc aggagtagta gatgtccacc tggtcgtcct
     6961 tcatgccgag gacccggcga tcccgctcct cgtagacgcg ctcggcctcc tccatcatct
     7021 ccaccacctt ctttccgtcg gtgatgaggg ttacctgcac ggcgtccagg agctcgggga
     7081 agtccatctt gaacttcgcc ccgatgagtt tccccaggtg ctccacccgc accccctttg
     7141 ccaccgcctc cttcgagagc cggacccaga tgaggttcct ctgggcgaca tgccaggagc
     7201 cctcgccgta gttcacgaag ttgtggatcc ttcgctcgag gaccggctcg aactccttcc
     7261 tcatccgttt ccccgtgacc tccacgagga tgccgagggg gatcgcggag ccctgcttca
     7321 tctccccaat ctcggggccg acgaccgtga cccgcccgtc cacgacctcc cccggttccc
     7381 tcatcctgag gagctcgaag gccggcgacc tcccgccccc gaactccacg tacatctcct
     7441 ccttccggat gctcttcccc tcgaaggcgg gggagcagcc catggggatg gggatggcgg
     7501 tcaccttcac ccggatgtcc ttctcctcca tcccccgccg gaccgcctcg tccgccgtgg
     7561 cgtggatcca cgatcccccc tcgtatcccc cgagggtgat gatggggacg gaaagggcgc
     7621 ggagggtgtc tgcaaaggcg atctcctcgt ctgagagacc ggggaagacg atgacaaaga
     7681 ccttggcacg ctcggcggcg taggcaatga gccttccggc gtcccccggg accaccccgc
     7741 cgaacatcat cgccacccgg gcgacgatgt ccgcgaagtg taccccgtgc aggggccgtg
     7801 agccgagggg aaccaggcgg taatcaaggc cggtcttcac cccggcagtg gtgagggtgc
     7861 ggatgatatc cccggcgagg aaggtgagca tgtacttctc ctggagctcg cgggccatcc
     7921 ccgccgcctc gtcggcggag gccggcgacc cggcgaggag ggccatcccc aggatggaac
     7981 cgtcgacgag ggagtacccg agcttccgga ggacggtgtc ggggatgaag ccggtgaagg
     8041 ggcctccctc ggacccggat gcggcggccc gggccgcgag gaggcactcg caggcgacga
     8101 gagggttctc ccctgacttt gcatatgcct cgcgggcgga cgggccgtcc ttcacatcga
     8161 tccccgtgag ggcatacgaa atggggaggt ggtacgcggt ctcggcgtac ccgagggttc
     8221 cggagatggc tgccatcgcc ttcttcagct gcccggttcc ctgtccgact cccgcgtcga
     8281 tctcgctcat tccctgtccc cgcttccact ctgtaatgga agagagagct taaaagtatt
     8341 cccgggcgaa ccgaacccgc cccatcccgc gtcccggcgc accgtgcccg ccccgactcc
     8401 atatcatccc gaaccgtccg gaggcgatat cgccctgtct tcctgggcca tcgtgtatcc
     8461 aaggtcagaa gagcgggata agccacggct accgggtaaa acgcgtgaaa aatccgtgta
     8521 cacccgcatt ggaaaacaaa gaggaaattc ccttgaaaaa gcatttttac tccagaatga
     8581 aggatttttt ccctgctccg gcgttcccat acatttaaaa aaaggaaagg gaaacactaa
     8641 gtcaatggtt cccgcgaaga tcttcttcac gaagggcgtc ggcatccaca aggacaagct
     8701 cgcctccttc gagctcgcac tcaggaaggc cggcatcgag aagttcaacc tggtccatgt
     8761 ctcaagcatc ctcccgcccg gctgccggca ggtctccgcc gacgatggac tgaagctcct
     8821 ccagcccggg cagatcgtct tcgtcgtcat ggctcggaac gagaccaatg aaccgaaccg
     8881 gctcgtatcg gcggccatcg gcctcgcccg cccccgtgcc caggacgatt acgggtacct
     8941 ctccgagcac cacaccttcg gggagacaga cgagaagtcc ggcgagtacg ccgaggacct
     9001 cgccgcgacc atgctcgcga cgacactggg catcgagttc gaccccgacc tcgcctggca
     9061 ggagcgcgag cagatcttca aggcgagcgg gaagatcttc aagaccaccc acatctgcca
     9121 gtcggcccag ggggagaaga ccggcaagtg gacgacagtc atctccgtcg cagttttcgt
     9181 tgaggacaac aacaaccaga ccaaactccc cctcggtcct cctcctccgc aagaacccga
     9241 tcagaagacg aagtagagga cgaggatggc tgcggtcgtc gcggccccga cgatcccaag
     9301 agctctcacc ctcggctcgc gcgcgagttt gagcgacacc aagcagatgg cgatgtaggg
     9361 gacaagggtg gtgaggacgg ccaccgaggc caggaggttg aaggagttgg agaagaagag
     9421 gaggcccccc gagaggatgc aggaaatgac gagggcggtc ttcggggtcc cccggggggt
     9481 gagctcggcg agccggggga gccggtaccg gcccgccatg ttctggagga cccgagaggt
     9541 cccgatgata taggcgttca gggccgagaa catggtgatg atcccgatga tggccaccag
     9601 gggggcggcc ccggagaaga cgagcccggc ggcggtggcg acaggtgcag gcgaggccgc
     9661 gaggtcagcc gatcccaccg cgccgatgag ggcgacgttg agcatgaggt agatggtgca
     9721 gacgaggagc atcacgagga ggagtgcgcg ggagatggtc cgggggtccc tcgtttcctc
     9781 gacggggatg gcgctgatct cgaagccggt gaagggccag tacacgatga tgaccacctt
     9841 caggatgtcc gggagggacg cggcccgggc gggtaccagg ttctcgggcc ggatgaaggg
     9901 gagaagggcc gccgcgatga ggacgatggg cacgatcttc gccgccgtga ggatgttctc
     9961 cgtccgcccc gagagggcga tcccgaccag gttgatcccg agaaaggcca tgatgacgaa
    10021 gatctccgcg gcgaggatgt gggggaccga gaagaagccg aagtactgcc cgagaccggc
    10081 cgcgatggtg gcgacgccga agaccgagga gaccaggtag acggtggcaa ggacccccga
    10141 ggcccggggg ccgaagaccg gttcgaagat ggcgacgaac ggggtcccgc ccggtgagcg
    10201 ggactggatc caggcgaggg agagcgcgac gcacaccgcc gagagggcga cgaagatcca
    10261 ggcaaagagt gacgacggtc ccgcgatgcc cgcggcgatc ccggggacca cgaagatgcc
    10321 ggaaccgatg gtccccccga ccccgaggct cacgagctcc acgaggccga gggatttccg
    10381 gtaagaaggc tccgccttcc cagagttatc caccgggaca cctccctgac ccgcagagta
    10441 ttgggacgcc cctgttatga acacaccggt ttcccggggg actgggcgga accccccaga
    10501 tgaaaatgcc tgcgggccca gtcttatctc cgggggatcc cgcactttca gggtgcccgc
    10561 ggcgaggatt tatccaggct ccgtgacgag tccctggcat gaacgtcaag gagaccctgg
    10621 agaccttcct cgaggagggg aaggactggg aacgcaagca gacctcgatt cgtggagtct
    10681 cgatcctgcg gatgccccgc accacgaccc ggccacccac tctagcgatg gagatcaatc
    10741 ccgtcgatga ccggggggca cagatgaaga gaaagggtgt catgatccgg agcccgaagg
    10801 agctcgactc cctgcggggg atcctctctg acccaaaggt cgtgaacctc atggaggctc
    10861 ttgcctccgt cgtcccaccc gtccctgcag caggaaagga accggcggca ggcgtcatag
    10921 aggtctagga accctgtgga gcaattgccc ggcatgggaa ggagcggatc cccgtgaaag
    10981 gaaacatcca ggaggacggc ccgggtcacc ggaccggaac tattcgaagg agaatcgtcc
    11041 gtagcggccg ccgccacccg ggaagagcct tacccttcct gtccgaagcc tggcgatggc
    11101 atcgccggcg acggggtgga gttcctgcaa ctcctccacc ggtacgtcca cgagcaccct
    11161 gatctccgtc ccgaaggccc cgaggaaggc atcgtagagt gcggccacct tcttcgtgtt
    11221 cgggctcgat gtcccgagga cgcgctggag gatctcggcg agcgggatca ggtggagata
    11281 cgggggtcgg ggctcaggcg gcatgtccga gagttcccgg gcccggtcgg agaccccctt
    11341 cttgatgagc ccgccgtcgt ccgggcaccg ccaggagagg gccgcggcct cctcgaggct
    11401 gaactcgcgg aagcaccggg tacaggcggt ccggttgtac ttcccctcct cggggaagaa
    11461 cccggcattc atggcgatcc ggcccttccg gatcgattcg aggacttctc cgacggtgcg
    11521 atgggggacc tcaatccggt tgaactccct ccccatcttc gcgggggagg ggctgtgggc
    11581 atcggagttg gagagaaagg gaattccccg gagctccgag attcccgcgc cgtaggagga
    11641 gtcggccgag aggccgagct ccaggaagtc ggggcgctcg tccccgtagc agtcccggac
    11701 atggtcaaag gaggcgtaga gggaggtcca gggggtgaag gcatgggccg ggccgatgag
    11761 cccccccgcg tcatgcacct ccctcgcgat ctcctccccg gcgagcctga gatggggccg
    11821 ccccatcgag gcgatggagg gggcagaggg ggaaaaccgc ccggcgagat ccccgaaggt
    11881 gtcaaaggat ccggcgagga tgaggtggtg gacgcggtgg gcgtcctcga cctcggcaga
    11941 cgggatgacg agaaccccgt cttctcgccc ggcctccccc cacatctcac gccatttccc
    12001 gtggagggcg tcaccgctcc ccaccgcggc gagtcccttt ctcaggcacc cggcgaggat
    12061 cgaggagggg accatcgagc gggaggaggc gatggagaac cgggaatgga tgtggagatc
    12121 ggtgtcgagg agcatggcct tcagccgaag taccggaggg cctcgtcatc gccgggcttc
    12181 tcgccctcgg tgagcttcgc gatgacctgc tccatctccg cggcgcgggt ctcgagccgg
    12241 ctccggtcga cctccacgcc ggtgagggcc gagaggacgg tgaggaggcc cgaggcggcc
    12301 cgggggtcca ccaggtaccc gctcgtctcc cccatcaggc agaccgcgtc catcccccgg
    12361 cggagggaga ggccgaggag gaggccggag gcgccgatga tcccccccgg cggctcgtcg
    12421 caggtgaact cggcccccgc cgcctcgacc ccggcgcgga gttccccgcg gttcaccgcg
    12481 gagaggaccc ggggttcgga gaccaggtgt cccaccccgt acccgcccag ggtgaagatc
    12541 ctccgcacac ccaggcgctc ggcgatgtcc aggtaggcct ccgcgaggag gtaatggccc
    12601 tcggccgaga cgctctggta atcgccgacg agaaaggcga tccccttgtc cccctcatgg
    12661 aggaagacct cgttcctcgc gagccgggcg actccgtccg gcccgatgat gacctggggc
    12721 gggaagtgga gggagtagat ctcggcgatc ctctcggccc ggagttcgtc gatgaggtac
    12781 tcggccacga gctttcccac atggccgatc ccggggagcc cctcgatgag gatctccgcg
    12841 gtcccgggct cctcagagag ccagcggacc tctatcgggt ccatccccgg ctcagcctcc
    12901 ggtaccttcc ccgccgatca tcgggggaga agcgggcggg atgggcggtc gcggtcggcc
    12961 tgccacagga ggggcagagg ggggagaggg tgtaggtgga gtcaatcggg cagcgccgga
    13021 tgcggccgct catgggatca gccggccttc tgcttgcgca cgaactcccc ggtgtccccg
    13081 gacttctcca tcacccggat ggcggccgtg gccgatcgct cgatggcctt ctcggcctgc
    13141 ttgtagtcgg gggcggtcac cttgatccgg tagatgggcg ccccgacgta gaggagctcg
    13201 atatcgacgt tctcgatctt cgggagggcg ctcttcaggg ccttccggat gacgctcacc
    13261 ccgtcgggct tcgtcgaggt gaggtgcagg tacccggcga tggtaacccg gggcttccgg
    13321 acgttctccc ccgcgatctt gagaaaggcg gcccgggcgg ggggtgggat ggcgagcttc
    13381 tcgaaaacct tctccccctc caccaccacg tcctcgaagg cccggtagag gtgggtgtag
    13441 tccttccgga tcgcgtcctc gatggccggg gccggcacct tcgactcctc agagacgaaa
    13501 ccgatccact tgtgggcctt ctgctcgttc ttccactcct ggatcttctc gcgcctctgg
    13561 tgctcgttca cgtcctttaa cgagaggtcg atgtggccgc ggctccggtc gacgtggagg
    13621 accttgcaga ccaccttctg cccctcccgg acatggtcgc ggatgtactt gacccagccc
    13681 ttcgcgatct ccgagatggg gatcaggccc tgccggccgc cgtactcgtc gagccggacg
    13741 aaggccacga agtccttcac cgcctcgacg gtgcagacga ccagttcccc ttcctcgggc
    13801 cagtccctct ctgccatcgg actctcactc gatctcggag aggatctcgg cggtgatgtg
    13861 cgccttgccc cccgccggct cggcgaggac ctttccgcag acgatgcagt ccacgacggt
    13921 cgaggccttg ccgaagatga cctgctcgtt ctcgcagtcg gggcacttca cccggtagaa
    13981 cctgctccgg ttctctcgat tcagacgtac cattctctca ctccgtgagc tcaaacttgg
    14041 agagccggaa cccggggcgc aggtgggcct tcttgcactt cgtgcaccgg tagcgcacgt
    14101 tgatcttctt cgtcggcttg tcgccgccgg ggaccttgga gaacttgccc atgttcccga
    14161 cctttcccct tcgcgccttc tgccggtcga tccagtggag gccccggctc ttgcccttct
    14221 tgaccctctc cacctcgtgg agctcgtgtt tccggcagaa agggcagtat gtgttgaatt
    14281 tcaccggcat cttcatgata ttggctccgt ttccctcgta acgagtacca taattattca
    14341 ccgggcatga tatttaaggg tatgcgatgc ccggtgccgg gtccttcctg gtgcccgggc
    14401 cgacgggatc cagtcgggtc ctgtgcctcc ggagaccccg ggcctaacct tcgggggcct
    14461 tcatcacgtc catcgcgata ttccggtcga tgaggacccc cgcgtttttc gagggcaggg
    14521 tgaccaggtc ctcggccttc aggttgtaca cccggccgtc gactcccatg aagggctcca
    14581 tgtccgagag gatccgcacg aggaccatgg cctcctccgg tggcatggcc ggcctcggga
    14641 ttcccgcggc ctccgggctc accccggcgg gaggtgtcac cccggggacg ccccctctcg
    14701 ggatctcgcc ggagagtgcc gccttcccct tctccagctc ccggatgatc ccgtcgaaca
    14761 tctccctctc cgcggggagc atctttttca cctcgtcccg ctcggcggtg ccggcctcga
    14821 cctgcacgag ggcgagggag aggatcttcc gtgaccggat gcggaagatg tcccgggcga
    14881 tctcccgcac cgccttcacc tcctcgatga gggtctgggc ctggtcggtg agggggtcac
    14941 tgcacccgta cacctgctcc tggagggagg cgaggtgccc acggatatgg tcgtagaggg
    15001 tcggggggac ctcggagagc cggccgctct cccgctcgtt caggatgacg atccgcaggt
    15061 catcgagggc catgcccgcc ctccccggcg atctccgcga ggccccgggc gcagaggaag
    15121 accgcgagcg cctccgggac ggcggaggtt cccttgtcca cctggtagcg gttcccgagg
    15181 atttccatgg aaatgttcgc ccccgattcc accttcaccg gccggtccga gaagaccacc
    15241 gcgtcccgga gggggtcgaa ggaatcgaaa tctccgaggg cgaggggggt gacccggaac
    15301 cccgatcccc cgtggaggga ggtgggcatc cggatgagcc tcctcgtgtc ggtggtcacc
    15361 ggctcgtcgg caagagcggc ccggccccgc agaagttcct ggaatttccc ccccgccgaa
    15421 atggctgcga aaacctggtt ttcggcgagc ggcccggagt ctcccctccc gagcccggcc
    15481 aggtacgtgt cgatggcgtc gaagaacctc ggggccctct ccctgccgat cccggggaac
    15541 tccatgagat gctccacccc cttctctcgc cccagcccct tgagccagag aaggtactcg
    15601 gagatggtgg atatatatct ctcgctccag cccggggtcc ctccccggac gccggagagg
    15661 agggcaccgg ggtcgatccc gatgccacag acatagtcga tgagctcccg acgctcctgg
    15721 ctcccccacc ccctcacacc aatatcccgg atgtggacat ggtagccccg cccgcccgaa
    15781 aacacgaggt gaatgatccg ctcggagaaa cccagctcgc cggtgagcat ggcgaggagc
    15841 ttctgggtct cgagcttcac gcggtcgagc atcgccctgt acgggccccg catgaggtgg
    15901 tcggcatcga ggtcgaagat gaggtctgcc ccggtccagc ccttctcggc catggttcct
    15961 gcccccggtg ccgtgtaata ggcgctcgag tagaaggcat gcgccgggac catggaccgg
    16021 aggtactccc cgagctcacc cgccgtgcca aaccccatgt gccggcgcat ccggacctcg
    16081 ggcgagtcgg ggtcaaagag gatgaacccg aactcccgct cccccaccga cgggggagcc
    16141 atgatccgct ccctccggta gtactccgtg aacctctgcc gaaggaactc ggtggtcgcg
    16201 ggcctcatgc cagctcctta accatatagg gccccctcct ctcatagcct ctcctccggt
    16261 agtacggccg gaccccgatc cccgggagga ccgtgatgcc ccggaagcct cccttccgtg
    16321 acaggtcctc cgccctttcg aggaggaccg atccgaagga ccggtgctgc cattcggcag
    16381 atcccgcgtc catcccgagg gggaccaggg ggccgtacac gtgaagctcg cggagcaggg
    16441 aggcctcctc cggcacggcc ttcccgggat cgccggggaa ccggagccgg gcatacccga
    16501 tgagggcctc cccggccacg gcggagatga agtgctcggt ccccccgcag cacggatagg
    16561 tgaggacctc gaacccgggc gttcctcccg gagtgcatct cccggcctcc cggcagcgga
    16621 tgcaccggca ggcccccccc atctctccga gccggctcct cgccatctgt ctcaggttcc
    16681 cgtggatgga accggcggtg atgagcttcg ccgggatgtc ccgctgcacc cgctggagcc
    16741 tgacgtacgg ggggagcaaa gccttggccc gggcgatgag gtcgacgagg accggctccg
    16801 ggtacggctc gtaaccaccg ttccggtaca gatcctcgat accggatccc ggggtcacga
    16861 gggtcgggta gatcttcagg aagtcgggcc ggaggtcttc atcggagaag agggtccgga
    16921 acattgcctc atcgtcggcg atggaggcac ccggaaggtt gggcatcacg tggaatccca
    16981 ccttgaatcc cgagtcccgg aggagccggt tcgcctcgac ggcgtccgcg aggctgcacc
    17041 ccctccggtt caggtcgagg atccggtcat cgacgtgctg gaccccgagc tccaccttgg
    17101 tgaccccgag ggagagcatc ctcttcacgt gttcttccct gcagtaatcg gggcgggtct
    17161 cgaaggtcac ggccacgcac cggacggccg aggtctcgtt cgcggcggcg atcctctcga
    17221 agggctcccc ggtttcaccg gtgacacccc ccgactcgtt catcgcacgg atgcactcgg
    17281 agacgaacca ctgctggtat tccggaggcc gggcggttat ggtccccccc atgacgatga
    17341 gctcggcctt gtccacgtga tgcccgaggg cccggaactg gtcgagacgg gccctcacct
    17401 gccggtacgg gtcatacccg tgctcacggg cgcggagggc ggcaggttcc tcaccggtat
    17461 aactctgggg tgtcccgaag ggatggtccg ggccgcccgg gcaggggagg cacttcccat
    17521 ggggacaggg agccggggag gtcatgaccg ccaccggggc gacccccgag agggtacggg
    17581 tgggtttcag gaggaggagt tcccgcagcc gttcacgctc ctccgggcgg gccgcggcaa
    17641 ggatggcgga gttcttcggg atcacggggg aatggtgggc ccggcagacc tcgatcttca
    17701 ggtgttccaa ccgggaaggg gaaaggggat gagaagagag gcgggagatg atctcccggc
    17761 aaagggcgaa ctcatccatg gattactgtt cggcggcgac gcgtcctgtc gcggcctcag
    17821 ggctcatctc caccatcgcc gggcggcggg ccctgatgtg agatacgatc tcatccccga
    17881 gggtgaggat ggccttcttg tggagaatct tgttcttgtg gatgtggacc ggtgatatgg
    17941 tcagggcatt gtacctgtcc gtgaagattt catcagaagt gaggttctca taatatttct
    18001 ttacatgaat gagtaacata tgtaagtgga gcaactcttc cttctgcacg ctatcaccat
    18061 ctttaactct acttgccgtt cctaaatata ttttattggt gatgtttata ttgcaagaag
    18121 agcgggcagt gggggtgctc ctggggctcg cggtagggga cgcgttgggt gcgccccttg
    18181 agggcctgcc tccaccgtcg gtgccggtca gggatctgcc ggccggaggg tcgtacaccg
    18241 atgacaccct ccaggccatg gccgtggcca ggtcgctcgt ggtctcccgc gggttctcac
    18301 ctgaggactg catggcccgg ctcctggatg ggtatcgcag gtacccggag ttctacggtc
    18361 cgacctccag cggggttttc gcccttgttt tggaaggggt gcccgcccgc gacgctgccc
    18421 tgcaggtaca tcggacactg ggcgggagcc ggtccaacgg gtcggtgatg cggggcccgc
    18481 ctctcgggat cttcaccgcc tcccccgttc tcgaggagat gagccttgcc tgctcgcgcc
    18541 tcacccactg cgatccggtc gcgggggcct gctctgcttt cgtgaaccgg atgatcgcga
    18601 gcctcgcgcg ggaggcttcg cgtggtgaag ccttccggct cgcctgctca acctgccgca
    18661 gcggcgaggt cctgtcgatg ctgggagagt accggaaaaa tccgcgggac ccatccctgg
    18721 atgcgctcct tgccacgcac tgcgccctct cggttttcat ggaatctgct tccttcgcag
    18781 aggcggtgct cggggcggta aacatgggag gcgatgccga taccatcggc gccatcaccg
    18841 gggctctcgc gggggcagcc tggggggcgg gggcagtccc ccctctgtgg cgggtgaacc
    18901 tgaaggacca tgaccggatc attgaactgg ccggggggct ctaccgggtg gcagtggaaa
    18961 agggggagtg aaacggcatc gaaaaggggg ttagagcggc ctcagctcaa ccagtttgag
    19021 ggcccggccc ttctcgcaga tgtccggcgc gttccccagg atctccccca ccaggtaccg
    19081 ctcgccgtcg atgaccccgt ccggccggca gagctcgaag gacttgcact cctccttgtt
    19141 gcaggagacc tcgaaggtg
//
LOCUS       DATGDI010000053         1936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_266691, whole genome shotgun sequence.
ACCESSION   DATGDI010000053 DATGDI010000000
VERSION     DATGDI010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266691"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VKO45_02065"
     CDS             <1..595
                     /locus_tag="VKO45_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04691.1"
                     /translation="AAFLVPGVGATGNGAMSGPHFNLNLIGKARTDQLPNDANNGARI
                     FVTLNGNSRIYLTLGTTFNVIDADATDGRGEFMLPAPGNIYNPDGTYSGPGNYQVFIR
                     AVGKPGGSGKISTCGENTVAGVTETLCSLDNVTLIRKKGKSTFQDVTRQLTTVYYYNA
                     TTMTYQRVDIFDDAFQDYLWSYDNLGMKNVQLRFYPI"
     gene            complement(782..1765)
                     /locus_tag="VKO45_02070"
     CDS             complement(782..1765)
                     /locus_tag="VKO45_02070"
                     /inference="COORDINATES: protein motif:HMM:NF028509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HMA04692.1"
                     /translation="MRTYTALFFSLLMVSILAAGCTAPGTPPQNQPIVTQNTTAMPVS
                     GSSVPPEQLVAFVERAYEFAHDHGKEAALREFNNPSGRFVQGELYIFAYDPEGNTLAL
                     PFQPDLLGKNRWNLTDANGTPFIREIIQAARSGGGFVRYFYADPSDNFRIKPKLSYVM
                     MVDEGWLIGSGVYKPPGDSLIVRVGSDPEVRGDLRSFVSEAVVYAAKHGKEAAIREFN
                     DRNGSFIRGNRYIYAFDYRGTTLALPFQPQLVGTDLSGLKDPFRVNYTRIEILLAQHG
                     GGFIFYHYLNPARNMTLEPKMSYIQKVDDTWWLGTGIYLQDWEDVITASPG"
BASE COUNT          415 a          547 c          587 g          387 t
ORIGIN      
        1 cgcggccttc ctcgtcccgg gagtcggggc gacgggcaac ggcgccatga gcggaccgca
       61 cttcaacctg aacctcatcg ggaaggcgag gaccgaccag cttcccaatg acgcgaacaa
      121 cggagcgagg atattcgtta ccctgaacgg gaacagcagg atctacctca ccttgggaac
      181 caccttcaac gtcatcgatg ccgatgcaac ggacggaagg ggcgagttca tgctccccgc
      241 tcccgggaac atctacaacc cggatggcac ttactcgggc cctggcaact accaggtgtt
      301 catccgggcc gtcgggaaac ctggcgggag cgggaagatc tcgacctgcg gggagaatac
      361 cgttgcgggt gtgacagaga ccctctgctc tctcgataac gtcaccctca tccggaagaa
      421 gggaaagtcg accttccagg acgtcaccag gcagctcacc accgtgtact actacaatgc
      481 aacgaccatg acctaccagc gggtcgacat ttttgacgat gccttccagg actatctctg
      541 gtcctacgac aacctgggta tgaagaacgt ccagctcagg ttctacccca tctgacggga
      601 atccgtaatt ttccttcttt ttcaggaggg ttttctaccc ccgagaggtg atggcagctc
      661 caaccggtct cacgtcaccg cagaagccgg ttgtcggggg tacacgtcgc cggaatgaaa
      721 gagaaaaacg ggggatctct tgagaagggg ggtcagaccg ccgttgaggg aaggcctgta
      781 tctatcctgg tgatgccgtg atgacatcct cccagtcttg gagatagatc cccgttccga
      841 gccaccaggt gtcatcgacc ttctggatgt aactcatctt gggttccagg gtcatgttgc
      901 gggccgggtt caggtagtgg tagaagatga acccgccacc gtgctgtgcg aggagaatct
      961 cgatcctcgt gtagttgacc ctgaagggat ccttgagtcc cgagagatcg gtccccacca
     1021 gctggggctg gaaggggagt gccagggtcg ttccccggta atcgaaggca tagatgtacc
     1081 ggtttccgcg gataaacgaa ccgttcctgt cgttgaactc ccggatcgcg gcctcctttc
     1141 cgtgtttcgc ggcatataca acggcctcgc tgacaaagga ccggagatcc cccctcactt
     1201 cgggatcgga ccccacccgg acgatcaggg aatcacccgg tggcttatag acgccggacc
     1261 cgatgagcca gccctcgtcc accatcatga cgtagctgag tttcggtttg attctgaagt
     1321 tgtcagaggg atctgcatag aaataacgga cgaaccctcc tccggaccgg gcggcctgga
     1381 taatttcccg gataaacggg gtgccgttgg catctgtcag gttccaccgg ttcttcccga
     1441 ggaggtcagg ctggaagggg agggccagcg tgtttccctc ggggtcgtag gcgaagatat
     1501 agagttcgcc ctgcacaaac cggccgctcg ggttattgaa ttcccggagg gctgcttcct
     1561 tcccatggtc gtgggcaaat tcgtaggccc tctcgacgaa ggccacgagc tgttccggcg
     1621 gcactgagga accggaaacg ggcatcgcgg tggtgttctg ggtcacaatc ggctggtttt
     1681 gcgggggagt acccggggcg gtacaaccgg cagcaaggat cgaaaccatc agcagcgaaa
     1741 agaagagggc cgtgtatgtt ctcatgaagg gcaatcgcgt taacgggtca taaaccatga
     1801 tagaatgaac cgggccatct cccgggtacc tggagggggc aacagtacgt gatcatcgtc
     1861 tccgtccggc tttcgggtag aaggagtggg cccgctgaga ttcgaactca ggatcttcgc
     1921 cgtgtgaaag cgacgt
//
LOCUS       DATGDI010000054         5146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_266702, whole genome shotgun sequence.
ACCESSION   DATGDI010000054 DATGDI010000000
VERSION     DATGDI010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266702"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..639
                     /locus_tag="VKO45_02075"
     CDS             19..639
                     /locus_tag="VKO45_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HMA04693.1"
                     /translation="MLERIPPREWRDLAVAWFAITLAFTLIFLGGGTSGITLAGFIFI
                     FALSSVTVGVGFILHELAHKFTAMHYGYWAEFRKDNLMLLVAVAMAALAGFVFAAPGA
                     TVIFGANLSRKENGLISASGPVTNLLLAIPFVGLALVGGGGSLLLLEIGDIGWKVNAM
                     LAAFNMIPVGGLDGRKVFAWNPGIFGLLIAAAFALLVLSYFGPLAP"
     gene            complement(615..1262)
                     /locus_tag="VKO45_02080"
     CDS             complement(615..1262)
                     /locus_tag="VKO45_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HMA04694.1"
                     /translation="MEPVKVLGISGSPHRHGNTETLLDTFLEGAESAGAGTRKVLLHE
                     MGSATCRGCNSCHKTGECVIRDELIPLFDEIFSVDVLALASPIYTMGITAEMKGFLDR
                     SQFLWAQKYILKTRYYPRDHIHRHKGFFISTAGTGWENVFSSAFPTMTAFFDIVGFEY
                     YDNIIAKDMDLYHGIRNHPTALAEAREKGGKVVGVVSALKEAPPEKESHGASGPK"
     gene            complement(1268..1879)
                     /locus_tag="VKO45_02085"
     CDS             complement(1268..1879)
                     /locus_tag="VKO45_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HMA04695.1"
                     /translation="MPFSVLAFATSPRRHGNSETLLDWVLAEMAGEPDVTIEKVALDE
                     VTVNPCRGCNACEVFGKCIQRDDMDFLYTKIVEADCIVFASPVYCLGICAQGKALVDR
                     AQVFRSRKYVLKLPVVAPEKLGKRVGIFISTAGQNWDIVFDGAKMSIRCFFHVTEIKD
                     RDTLYLLVRGVDKAGEVETHPTARDEARAIGKKAVERLREMNR"
     gene            complement(1949..2389)
                     /locus_tag="VKO45_02090"
     CDS             complement(1949..2389)
                     /locus_tag="VKO45_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMA04696.1"
                     /translation="MVRKVCIIDDNEECLMEKTKKETTLHQQKPGPGADKADGESAVL
                     AKIAEMKGPYRAMGERVHAIIKASAPALSPNLWYGMPAYARDGDVICFFRSGEMFKER
                     YMTLGFNSKANLDEGAMWPIAFALKELTAAEEARIAALVKKAVS"
     gene            2388..2753
                     /locus_tag="VKO45_02095"
     CDS             2388..2753
                     /locus_tag="VKO45_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMA04697.1"
                     /translation="MGEENSPPAPPDGDARFNHYLSMLSDEDPSRRWKALEALARLGD
                     PRAFQPVVNALKDPDWRVRRKAAWALGVLGDPRAVIPLRRAMLNEMDSVKEMIEEAMQ
                     AIARKALGGEDWVNPFEKE"
     gene            complement(2767..3408)
                     /locus_tag="VKO45_02100"
     CDS             complement(2767..3408)
                     /locus_tag="VKO45_02100"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HMA04698.1"
                     /translation="MPEEPVSFPVIGEPAPDFEADTTQGLITLSSLKGKWVVLFSHPA
                     DFTPVCTTEFLAFAKIHDELAALNVQLIGLSVDSVSSHLAWVRNIREKMGVTIPFPII
                     ADLTMKVAKRYGMIHPGASTTAAVRCVFFIDDKGILRAMIYYPLQNGRYMPEILRLVK
                     ALQTTDQYKVSTPANWQPGEKVVVPAPKTVADMEKRLSEGYDCKEWYLCFKKI"
     gene            complement(3415..3816)
                     /locus_tag="VKO45_02105"
     CDS             complement(3415..3816)
                     /locus_tag="VKO45_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMA04699.1"
                     /translation="MAVRKTSKIPAGKSKNTGSSKGMRELEKKIGKVPVFFRELMKTQ
                     PDMHGAILAFDQHIWDDGQLSRKTKKILAIAIAAALRDQHAVKAQLAGAKKLGVSREE
                     IEEALRVTFLLSGMPAYVYGRAAMEEVMGRE"
     gene            complement(3821..3946)
                     /locus_tag="VKO45_02110"
     CDS             complement(3821..3946)
                     /locus_tag="VKO45_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HMA04700.1"
                     /translation="MVNVSAVGQVFECMICGNVVEVQAVGGGELVCCGEPMVERK"
     gene            complement(3956..4084)
                     /locus_tag="VKO45_02115"
     CDS             complement(3956..4084)
                     /locus_tag="VKO45_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HMA04701.1"
                     /translation="MVNVREAGEVYVCHVCGNTVVVREAGGGELVCHGEPMVRKEA"
     gene            complement(4153..4389)
                     /locus_tag="VKO45_02120"
     CDS             complement(4153..4389)
                     /locus_tag="VKO45_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04702.1"
                     /translation="MAKGTAKAAKAGAKGEPGKYVCIDCGYEFELSARDKELKKCPMC
                     ACEEFNCFPMVAMRPDIKTPEDVKNPPKKREYLK"
     gene            4554..>5146
                     /locus_tag="VKO45_02125"
     CDS             4554..>5146
                     /locus_tag="VKO45_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HMA04703.1"
                     /translation="MGKKVIALLGSPLPEGNTAALLDRAIRGAEEAGCTVEKIVVPFL
                     DFEPCQEILFCREHETCQMEDDLTPLYQKFRELDGLIIATPIMTMGIPGKLKSLMDRF
                     QVFFMAKYMRRDPLVPKEKRNSRLCLFIAISGMKVPGVFDGAKATVAAFLEIIDCRLF
                     GEILVPDMDTIRGIHTKPELLDQAYRKGLELGKALSG"
BASE COUNT          887 a         1648 c         1554 g         1057 t
ORIGIN      
        1 gtccaagccg gatccgcaat gctcgaacgg atccctcccc gcgagtggcg cgacctcgcc
       61 gtcgcctggt tcgccatcac cctcgccttc accctgatct tcctcggcgg ggggacctcc
      121 gggatcaccc tggccgggtt catcttcatc tttgccctct cctcggtgac cgtgggggtg
      181 ggtttcatcc tccacgagct cgcccacaag ttcacggcga tgcactacgg gtactgggcg
      241 gagttccgga aggacaacct gatgctcctc gtggcggtgg cgatggcggc cctcgccggc
      301 tttgtctttg cggccccggg ggcgaccgtc atcttcgggg cgaacctctc ccggaaggag
      361 aacggcctca tctcggcatc gggacccgtc acgaacctgc tcctcgccat ccctttcgtc
      421 ggcctcgccc ttgtcggcgg tgggggatcc ctcctcctcc tcgagatcgg ggacatcgga
      481 tggaaggtga acgcgatgct cgcggccttc aacatgatcc ccgtgggtgg cctcgacggg
      541 agaaaggtgt ttgcctggaa cccggggatc ttcgggctcc tcatcgctgc ggccttcgcc
      601 cttctcgtcc tctcctactt cggcccgctt gccccgtgag attccttctc cgggggagct
      661 tccttcaggg ccgagaccac ccccaccact ttccctccct tctcccgggc ctcggcaagg
      721 gccgtcgggt ggttccggat cccgtggtag aggtccatgt ccttggcgat gatgttgtca
      781 tagtactcga agccgacgat gtcgaagaag gcggtcatgg tagggaaggc cgaggaaaag
      841 acattctccc agcccgttcc cgcggtggag atgaagaacc ccttgtgccg gtggatatgg
      901 tcgcgggggt agtaccgggt cttcaggata tatttctggg cccagaggaa ctgggaacgg
      961 tccaggaagc ccttcatctc ggcggtgatc cccatggtgt agatggggga ggcaagggcg
     1021 aggacatcca ccgaaaatat ctcatcaaag agggggatga gctcgtccct gatgacgcac
     1081 tccccggtct tgtggcagga attgcacccc cggcaggtgg ctgagcccat ctcgtggaga
     1141 aggaccttcc gggtgccggc ccccgccgat tcagctccct cgaggaaggt atcgaggagg
     1201 gtctcggtgt tcccgtgccg gtgggggctt cccgagatgc cgaggacctt cacgggctcc
     1261 atccggttca ccggttcatc tcgcggagcc tttcgacggc ctttttcccg atggcccggg
     1321 cctcgtcccg tgccgtcggg tgggtctcca cctcgccggc cttgtccact ccgcgcacga
     1381 ggagatagag ggtatcccgg tcctttatct cggtgacatg gaagaagcac cggatggaca
     1441 tttttgcacc gtcgaagacg atgtcccagt tctgccccgc ggtggagatg aagataccga
     1501 cgcgcttccc gagtttttcc ggcgccacca ccgggagctt gaggacatac ttccgggacc
     1561 ggaagacctg cgcccggtcc acgagggcct tcccctgggc gcagatgccc aggcagtaga
     1621 cgggcgaggc gaaaacgatg cagtccgcct cgacgatctt cgtgtagagg aagtccatgt
     1681 catcccgctg gatgcacttc ccgaagacct cgcaggcatt gcagccccgg caggggttca
     1741 ccgtgacctc gtcgagggcc accttctcga tggtgacatc cggttccccg gccatctcgg
     1801 cgaggaccca gtcgaggagg gtctcggagt tcccgtgcct ccgggggctc gtcgcaaagg
     1861 cgaggaccga gaacggcatg ctccaatgct tggtcgcggg ccttaaaaaa ggctccacct
     1921 tcgaccggcc cggtggcgag ctctgtcctc agctcactgc tttcttcacg agcgctgcaa
     1981 tccgtgcctc ttcggcggca gtcaactcct tcagcgcgaa ggcgatcggc cacatggcgc
     2041 cttcgtcgag gttcgccttg ctattgaagc cgagcgtcat gtacctctct ttgaacatct
     2101 ccccgctgcg gaagaagcag atgacatcgc cgtccctggc atacgcgggc atcccgtacc
     2161 agaggttcgg cgagagggct ggggcgctgg ctttgatgat ggcatgaacc cgctcgccca
     2221 tggcgcgata cggtcccttc atctcggcga tcttcgcgag cacggcgctt tccccgtccg
     2281 ccttgtccgc gcccgggccg ggcttttgtt gatggagagt tgtttctttc tttgtttttt
     2341 ccataaggca ctcttcatta tcatctataa tacatacctt cctcaccatg ggagaggaga
     2401 actctcctcc ggcaccaccg gacggagatg cccggttcaa ccactacctc tccatgctct
     2461 ccgacgagga cccatcgcgg cggtggaagg cgctggaggc cctcgcccgc ctcggcgatc
     2521 cccgggcctt ccagcccgtg gtcaacgccc tgaaggaccc ggactggagg gtgcggagga
     2581 aggccgcctg ggcgctgggg gtcctcggcg atccccgggc ggtcatcccc ctccggaggg
     2641 cgatgctcaa cgagatggac tcggtgaagg agatgatcga ggaggcgatg caggccatcg
     2701 cgaggaaggc gctggggggc gaggactggg taaacccgtt cgaaaaggag taggagggat
     2761 tgaagatcag atcttcttga agcagaggta ccactcttta cagtcgtacc cctcggagag
     2821 gcgcttctcc atgtccgcca cggtcttcgg ggccgggacc acgaccttct cccccggctg
     2881 ccagttagcg ggcgtcgaga ccttgtactg gtcggtggtc tggagggcct tcacgagccg
     2941 gaggatttcg ggcatgtatc tcccgttctg gagcgggtag tagatcatcg cccgcaggat
     3001 ccccttatca tcgatgaaga agacgcagcg aacagccgcc gtcgtagagg cgccggggtg
     3061 gatcatcccg taccgcttcg ccaccttcat ggtgaggtcg gcgatgatgg ggaagggaat
     3121 cgtaacgccc atcttctccc ggatgttcct cacccaggcg agatgcgacg agacgctgtc
     3181 cacggaaagg ccgatgagct ggacgttcag ggccgcgagt tcgtcatgga tcttcgcgaa
     3241 ggcgaggaac tccgtggtgc agaccggggt gaaatccgcg ggatgggaga agagcaccac
     3301 ccacttcccc ttgagggacg agagggtgat gaggccctgg gtcgtgtctg cctcgaaatc
     3361 gggcgcaggt tcccctatga cggggaagga gaccggctcc tcgggcatga ggatctactc
     3421 ccttcccatg acctcttcca tcgcggccct gccatagaca taggcgggca tccccgagag
     3481 gaggaaggtg acccggagag cctcctcgat ctcctccctg ctcaccccga gcttcttcgc
     3541 cccggcgagc tgcgccttca ccgcgtgctg gtccctgagg gccgccgcga tggcgatggc
     3601 gaggatcttc ttcgtcttcc gggagagctg gccgtcgtcc cagatgtgct ggtcaaacgc
     3661 gaggatcgcc ccgtgcatgt ccggctgggt cttcatcagt tccctgaaaa agaccgggac
     3721 cttcccgatc ttcttctcga gctcccgcat tcccttcgag ctcccggtgt tcttcgactt
     3781 tcctgctgga atctttgaag tctttcttac cgccatatca tcacttcctc tccaccatgg
     3841 gttcaccgca gcagacaagt tccccgccgc cgactgcctg gacctcgacc acgttcccgc
     3901 agatcatgca ctcaaagacc tgcccgaccg ccgagacatt caccatggga acctctcacg
     3961 cctcctttcg caccatgggt tcgccgtggc agacgagctc gccgcccccg gcctccctga
     4021 caacgaccgt gttcccgcag acatggcaga cgtagacctc acccgcttcc ctcacattca
     4081 ccatgatttc cctccattcc cgagcctccg ctcacccgtg gagagtaggg cccgggggct
     4141 ataaaaaggt gattacttca ggtactcccg cttcttgggc gggttcttga catcctcggg
     4201 ggtcttgatg tcgggccgca tcgcgaccat ggggaagcag ttgaactcct cgcaggcgca
     4261 catgggacac ttcttcagct ccttatccct ggccgagagt tcgaattcgt acccgcaatc
     4321 gatgcagaca tatttaccgg gttcaccctt ggccccggcc ttcgccgcct tcgcagtgcc
     4381 ttttgccata gtttaccctt ctctgtcttg atgcgtatct tatttatcgg tttctaaggt
     4441 ttccaaagag tgtcttgagg ggcagggttc ccgccggagt tttttcgggg atcctccggg
     4501 ccggccggaa aaaaagggga gggtttaaac catccgggtg catggaggat gctatgggaa
     4561 agaaggtcat cgccctcctc gggagccccc tccccgaggg taataccgcc gccctccttg
     4621 accgggcaat ccggggggcg gaggaggcag gctgcacggt agaaaagatc gtggtcccct
     4681 tcctcgactt cgagccctgc caggagattc tcttctgccg ggagcacgag acctgccaga
     4741 tggaggatga tctcaccccc ctctaccaga agttccggga gctggacggg ctcatcatcg
     4801 cgacgcccat catgaccatg ggcattccgg ggaagctcaa gtccctcatg gaccggttcc
     4861 aggtcttttt catggccaag tacatgcgga gggaccccct cgtcccgaag gagaagagga
     4921 actcccggct ctgcctcttc atcgctatct ccgggatgaa ggtcccggga gtcttcgacg
     4981 gcgcaaaggc caccgtggcc gccttcctcg agattatcga ctgccggctc ttcggggaga
     5041 tccttgtccc ggacatggat accatacggg gcatccatac gaaaccggag ctcctcgatc
     5101 aggcataccg gaagggtctc gagctcggga aggccctgtc aggata
//
LOCUS       DATGDI010000055         1516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_266935, whole genome shotgun sequence.
ACCESSION   DATGDI010000055 DATGDI010000000
VERSION     DATGDI010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266935"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VKO45_02130"
     CDS             complement(<1..1496)
                     /locus_tag="VKO45_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HMA04704.1"
                     /translation="MAMRVKDRGIWQSYTWKDYYEKVKYFSLGLIKMGLERGDKVSIL
                     GENKPEWYWAELASHCAGAPVVGIFTDCIPSEVKYYLEHSDSKFVVAHDQEQVDKLLE
                     IKGQLPLLKKVIYWDPKGLWHYRDPLLLSFEEVLKLGEVQEKENPQLFEQKIDEGQSS
                     DIGVICYTSGTTGLPKGAMLSQRWLVDGVRAWASMDDWNEKGCQYLSFIPPAWATEQG
                     LGIGGSLVADVSVNFPEEPETVQENLREIGPQILFYGARLWENVNRMVQARMIDSSAI
                     RRLLYRICLPLGYRMADIVGAGKTPSWFDRILYVLAYQTIFRALKDKLGLSNARCVYS
                     AGAAVSPEIIRFFQALGVQIKLFYGSTELGVVSIPRGKEIRPETSGPIVPWAEVRLSD
                     DGEILVKSQFMYSGYYKDPEATARKLKDGWYATGDFGHIDEKGQLIVIDRMEDLKTLA
                     GGKKFSPQYTEVRLRFSPYIKDVLVVGGEDKEFVTALVNIELENMGRYA"
BASE COUNT          287 a          477 c          418 g          334 t
ORIGIN      
        1 gcgtagcgcc ccatgttttc cagctcgatg ttgacgaggg cggtcacgaa ttccttgtcc
       61 tctcccccga ccaccaggac gtccttgatg tacggactga agcgcaatcg gacttcggta
      121 tattgggggg agaacttctt ccctccggcc agtgtcttca ggtcctccat ccggtcgatg
      181 acgatgagct gccccttttc gtcgatatgc ccgaagtccc ccgtggcgta ccatccgtct
      241 ttcagcttcc gggcggtcgc ctcgggatcc ttgtaatagc cggagtacat gaactggctc
      301 ttcaccagga tttccccgtc atccgagagc ctgacctcgg cccagggaac gatggggccg
      361 gaggtttcgg gacggatctc tttcccccgg ggaatcgaaa cgacccccag ttccgtactc
      421 ccgtagaaga gtttgatctg aacccccagg gcctggaaga agcggatgat ctccgggctg
      481 accgcagccc cggcggaata cacgcaccgg gcgttggaaa ggcccagctt gtccttgagc
      541 gcccggaaga tggtctggta agccaggacg tataagatcc gatcgaacca ggagggcgtc
      601 ttccccgccc cgacgatatc cgccatccgg tatccaagag ggaggcagat ccggtagaga
      661 agccggcgga tggccgaaga atcgatcatc cgggcctgta ccatccggtt cacgttctcc
      721 cagagccggg cgccgtagaa aagaatctgg gggccgattt ccctcaggtt ctcctggacc
      781 gtttccggtt cttcggggaa attcacggac acgtccgcca ccagcgagcc gccaatcccc
      841 aggccctgct ccgtcgccca ggccggaggg atgaaggaaa ggtactggca ccccttttcg
      901 ttccagtcgt ccatcgaggc ccaggccctt acgccgtcca ccagccacct ctggctgagc
      961 atggctccct tgggcagccc ggtggtcccg gaggtatagc agatgacccc gatgtccgaa
     1021 ctctgcccct cgtcgatctt ctgctcgaaa agttggggat tttctttctc ctggacttcg
     1081 ccaagtttca ggacttcctc gaaggacaga agaagcgggt cccggtaatg ccagagcccc
     1141 ttcggatccc agtagatgac ctttttcagc aggggaagct gacccttgat ctccagcagc
     1201 ttgtccacct gttcctggtc atgggccacc acaaatttgg agtcggagtg ctccaggtaa
     1261 tatttcactt ccgagggaat gcaatcggtg aagatcccca ctacaggggc tcctgcacag
     1321 tgggacgcca gctcggccca gtaccattcc ggcttgttct ctccgagaat ggataccttg
     1381 tctcccctct ccaggcccat cttaatgagt ccgagggaga agtatttgac tttttcgtaa
     1441 tagtccttcc aggtgtaact ttgccagatg ccccggtcct ttacccgcat ggccatcttg
     1501 cggtcgccgt tcttct
//
LOCUS       DATGDI010000056         4927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_412362, whole genome shotgun sequence.
ACCESSION   DATGDI010000056 DATGDI010000000
VERSION     DATGDI010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412362"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VKO45_02135"
     CDS             complement(<1..713)
                     /locus_tag="VKO45_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HMA04705.1"
                     /translation="MGGDGSAGRILRVNLTERAVGEVPFPKEWRARYLGGRGLGVRLV
                     YDLVDPLADPLGDGNAVVFAAGPLTGTGMPLGSRYCVVTKAPLTGTLSSSNSGGFFGT
                     EMRRTGIDAILVEGRAPGPVYLWIRDGSCEIRDAAPYWGMTTYETMEGIGRDLGEEKA
                     RIACIGPAGERLGRMACIVNDHGRAAGRGGMGAVLGSKRLKAIAVRGTGKVPRGDPAE
                     FSRVRDAIREKIEKAGLNKG"
     gene            822..1985
                     /locus_tag="VKO45_02140"
     CDS             822..1985
                     /locus_tag="VKO45_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HMA04706.1"
                     /translation="MPDVRVYTTTQCPYCRMVKAFLEKRGIPFSEINVSEDREAAGEM
                     IRISGQNGVPVTIAGDRVIVGFDAQALQEVFGGGEAPVDLFDVIILGAGPAGLAASVY
                     TARKFLKTLVITENIGGQALESWAIENYMGYRMITGEDLMKKFEEQAKELHIQLELDG
                     VIGISRDGSSFTVKTATDRSFGAKSILIATGKHPKMLGIEGEERFIGRGLSICATCDG
                     PLFRGKNVAVVGGGNSALQTAIEMSKIAKTVHLLVHSQIRCDAVYKDLYAKRENILTH
                     LNVEVARLYGDQFLTGLGIRHRDTGEEARIDVDGVFLEIGWLPNTEFLEGFVPLNPEK
                     EVVVDENCHTNVPGVFAAGDVTSVKGKQIIIAAGEGAKAALEVYEYLVRSGQG"
     gene            complement(2009..3859)
                     /locus_tag="VKO45_02145"
     CDS             complement(2009..3859)
                     /locus_tag="VKO45_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMA04707.1"
                     /translation="MTGPERKSGGAGQSRTEVPEIRVLIALSEPDLMEYARNDLQSEG
                     CFRVEVARSGEDALERLKSAGYDAIVSGMPLPGMSGVGLLKSIRCGGSRTPFIFLSRG
                     SDLKTVVEAFNQGTSAFIDTGTEGGLLPGNLCMAVLRAVEDHRSAVGVRKERDQLRII
                     LSAQRTGLALVNPDLTIAWANEEARRLFPGREPVGMPCHEFYEGREMPCDPCPTLRAF
                     GTGSVQETEHYNPLDQRWYHIVTQPVRDAEGRVIQVLEGITDITVRKQGEEALRLTQE
                     KYRELVENAGSIIFRSDREGNITFFNDFAQRFFGFSAPEILGKNVIGTIVPESDSAGK
                     DLRAMIREIREHPEEYRNNENENIKKNGERAWISWTNRPIYNGKGEFVELLSVGNDIT
                     ARKRAEEALSAANQKLNLLSNVTRHDVLNQLNVLSGYLGLVRDRISDVDTAALLSRAW
                     DSAWNIRKLIAFTKDYQEIGVHSPRWHEIPATVNRAASSLDLAGIALEVEVGDLQVYA
                     DPLLEKVFYALLDNALRHGDRIDRIRVSSHPEGREIALVVEDNGIGIPDGEKEKAFGR
                     GTGRNTGYGLFIAREILSITGLTIRETGTFGQGARFEIRIPEGAFRSGKG"
     gene            complement(3856..>4927)
                     /gene="tfrB"
                     /locus_tag="VKO45_02150"
     CDS             complement(3856..>4927)
                     /gene="tfrB"
                     /locus_tag="VKO45_02150"
                     /EC_number="1.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043830 - thiol-driven fumarate reductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase (CoM/CoB) subunit TfrB"
                     /protein_id="HMA04708.1"
                     /translation="LACVSVCPAMEVAEFAGPLTMRQEMRLAIDPRDSCDRISDAIRD
                     GLFMCTTCQHCWKVCPKKIEIPGKAIEKLRRMANKRGLTLPRHKEVAELVRETGRSVP
                     KNQPTFLDQVPEVIEPAGPVRCTVGFFVGCMYNNRIPQTALDAMEVLARNGIRVIIPH
                     EQVCCGSPLIRTGQTDILPDLMEKNIAAFTCRGIDTVVTMCAGCGSTLKNDYRTPFTV
                     KDVNELLCEIGIEEPARLPIRVAYHDPCHLLRGQGISRQPRELIGKVAELVELPVKCC
                     GAGGGTRSGQPEVSKALAAKKEEEIEKAGVECVVSSCPFCEFHIGEHSKRPVKNVTTL
                     LLEGYRRKDQDRSGEAGEPAAA"
BASE COUNT          800 a         1638 c         1612 g          877 t
ORIGIN      
        1 cctttgttca gccccgcctt ttcgatcttc tccctgatgg cgtcccggac ccttgagaat
       61 tcggccggat ccccccgggg aaccttcccc gttccccgca cggcgatggc cttcaggcgc
      121 ttcgacccga ggaccgcccc catccccccc cggcccgcgg cccgtccgtg atcgttcacg
      181 atgcaggcca tccggcccag ccgttccccc gcgggcccga tgcaggcgat cctcgccttc
      241 tcctccccga gatccctccc gatcccctcc atggtctcgt aggtggtcat cccccagtac
      301 ggggccgcgt cccggatctc gcaggagccg tcccggatcc agaggtatac cgggccgggt
      361 gccctcccct ccacgaggat ggcatcgatg cccgtccgcc tcatctccgt cccgaagaac
      421 ccgccgctgt tgctggaaga gagggtcccg gtgagggggg ccttggtcac gacgcagtac
      481 cgggatccga ggggcattcc cgtgccggtg agtggcccgg cggcgaaaac cacggcgttc
      541 ccgtcaccga gagggtcggc caggggatcg acaaggtcgt agacgagccg gacgccgagc
      601 ccccgacccc cgagataccg tgccctccat tccttcggga acgggacctc tccgacggcc
      661 ctttccgtga ggttcacccg gaggatccgt cccgcgcttc catctccgcc catggcgaag
      721 ggtacggtgg gaagggagac ggataaacat atcccgggat ccggccctcc ggccgccctt
      781 tcagataccg ctttatatga gcagggaaga gtaccgtggc aatgccagac gtccgggtct
      841 ataccacgac gcagtgcccc tactgccgca tggtgaaggc tttcctggag aaacggggca
      901 tcccgttctc cgagatcaac gtctccgagg atcgtgaggc ggccggcgag atgatccgca
      961 tctccggcca gaacggggtt cccgtgacca tcgccgggga ccgggtcatc gtgggcttcg
     1021 atgcccaggc cctgcaggag gtcttcggcg gcggcgaggc ccccgtggat ctgttcgacg
     1081 tgatcatcct gggagccggg ccggccggcc tcgccgcctc ggtctacacc gcgaggaaat
     1141 tcttaaagac cctggtcatc accgagaaca tcggcggcca ggccctggag agctgggcca
     1201 tcgagaacta catgggctac cggatgatca cgggcgagga cctgatgaag aagttcgagg
     1261 agcaggcaaa ggagctccac atccagctcg agctggacgg ggtgatcggc atctcccggg
     1321 acggctcctc cttcaccgtg aagaccgcga ccgaccggtc cttcggggcg aagagcatcc
     1381 tcatcgcgac cgggaaacac ccgaagatgc tcgggatcga gggcgaggag cggttcatcg
     1441 gccgggggct ctccatctgc gccacctgcg acgggccgct cttcaggggg aagaacgtgg
     1501 ccgtggtggg gggagggaac tcggccctcc agacggccat cgagatgagc aagatcgcaa
     1561 agacggtcca cctcctcgtc cattcccaga tcaggtgcga cgccgtgtac aaggacctgt
     1621 acgcgaagag ggaaaacatc ctcacccacc tgaacgtgga ggtcgcgcgg ctctacgggg
     1681 accagttcct caccggcctc ggcatccggc accgggacac gggggaggag gcccggatcg
     1741 acgtcgacgg ggtcttcctc gagatcgggt ggctcccgaa caccgagttc ctcgagggat
     1801 tcgtgcccct gaaccccgag aaggaggtgg tggtggacga gaactgccac acgaacgtcc
     1861 cgggggtctt tgcggccggc gatgtcacga gcgtcaaggg gaagcagatc atcatcgcgg
     1921 ccggggaggg ggcaaaggcc gccctcgagg tgtacgagta cctcgtgagg agcgggcagg
     1981 ggtagacccg cccggccggg ctatcctttc agcccttccc ggaacggaag gcgccctcgg
     2041 gtatccggat ctcgaaccgg gcaccctgcc cgaaggtgcc ggtctcccgg atggtgaggc
     2101 ccgtgatgga gaggatctcc ctcgcgatga aaaggccgta gccggtgttc ctgcccgtac
     2161 ccctgccgaa ggccttctcc ttctctccgt cgggaatccc gatcccgttg tcctccacga
     2221 caagggcgat ctcccttccc tcggggtggc tggagacacg gatccggtcg atgcggtcgc
     2281 cgtgccggag ggcgttgtcc aggagtgcgt aaaagacctt ttcgaggagg ggatcggcgt
     2341 aaacctggag gtcccccacc tccacctcga gggcgatgcc cgcgagatcg agggatgagg
     2401 cggcgcggtt cacggtcgcg ggaatctcgt gccaccgggg cgagtggacg ccgatctcct
     2461 ggtaatcctt cgtaaaggcg atgagcttcc ggatgttcca ggccgagtcc caggcccggg
     2521 agaggagcgc cgcggtgtcc acatcgctga tccggtccct gacgaggccc aggtagccgg
     2581 agaggacgtt cagctggttc agcacgtcgt gccttgtcac gttcgagagg aggttcagct
     2641 tctggttcgc ggcagagagg gcctcctcgg cccttttccg ggcggtgatg tcatttccca
     2701 ccgagaggag ctccacgaac tccccctttc cgttgtagat cggacggttg gtccaggaga
     2761 tccaggcccg ttcgccgttc ttcttgatgt tctcgttctc gttgttccgg tactcctccg
     2821 ggtgctccct gatctcccgg atcatggccc ggaggtcctt ccccgcgctg tcgctctcgg
     2881 ggacgatggt gccgatgacg ttcttcccga ggatctcggg ggccgagaag ccgaagaacc
     2941 tctgggcgaa gtcattgaag aaggtgatgt tcccctcccg gtccgagcgg aagatgatgc
     3001 tccccgcgtt ctccacgagc tcccggtatt tttcctgggt gagccggagg gcctcctcgc
     3061 cctgcttccg gaccgtgatg tcggtgattc cctcgaggac ctggatcacc cgcccttcgg
     3121 cgtcccggac cggctgggtg acgatatggt accacctctg gtccagggga ttgtagtgct
     3181 cggtctcctg gacgctcccg gtcccgaagg ccctgagggt ggggcaggga tcgcacggca
     3241 tttcccggcc ctcgtagaac tcgtggcagg gcatccccac cggctccctt cccggaaaga
     3301 gcctccgagc ctcctcgttc gcccaggcga tggtgaggtc cgggttgacg agggcgagcc
     3361 cggttctctg ggcggagagg atgatccgca gctggtccct ctccttcctt accccgacgg
     3421 cggaccggtg atcctcgacg gcccggagga cggccatgca gaggtttcca gggaggaggc
     3481 ccccctccgt cccggtatcg ataaaggccg aggtgccctg gttgaaggcc tcgaccacgg
     3541 tcttcaggtc tgatccccgg gaaaggaaga tgaagggagt ccgggaccca ccgcaccgga
     3601 tgctcttcag gagaccgacg ccactcatcc cggggagagg catgcccgat acgatggcat
     3661 cgtagcccgc cgacttcagc ctctccaggg catcctcccc ggaccgggcg acctcgacac
     3721 ggaagcatcc ctcagactgg agatcgttcc ttgcgtactc catgagatcc ggctctgaga
     3781 gggcgatgag gacccggatc tccgggacct cggtcctcga ctgcccggcc ccacccgatt
     3841 tcctttccgg tccggtcatg cggccgccgg ttcgccggct tccccactcc tgtcctggtc
     3901 cttccgccgg tagccctcga ggaggagggt ggtcacgttc ttcaccggcc tcttcgagtg
     3961 ctcgccgatg tggaactcgc agaaggggca ggaggagacg acgcactcca ccccggcctt
     4021 ctcgatctcc tcctccttct ttgccgcgag agccttcgag acctcgggct ggccggaccg
     4081 tgtcccgccc cccgcgccgc agcacttcac cggcagctcg acgagctccg ccaccttccc
     4141 gatgagctcc cggggctgcc tcgagatccc ctggccccgg aggagatgac atgggtcgtg
     4201 gtaggctacc cggatgggga gccgggccgg ttcctcgatc ccgatttcgc agaggagctc
     4261 gttcacgtcc ttcacggtga agggagtgcg gtagtcgttc ttcagggtcg agccacagcc
     4321 ggcgcacatc gtgacgacgg tgtcgatgcc ccggcaggtg aaggcggcga tgttcttctc
     4381 catgagatcg gggaggatat cggtctggcc ggtccggatg aggggagagc cgcagcagac
     4441 ctgttcgtgg gggatgatca cccggatccc gttccgggcg aggacctcca tggcatcgag
     4501 ggccgtctgg gggatacggt tattgtacat gcagcccaca aagaacccga cggtgcaccg
     4561 gaccgggccc gcaggttcga tgacctccgg tacctggtcg aggaaggtcg gctggttctt
     4621 cgggaccgaa cggccggtct cccggacgag ctccgccacc tccttgtgcc tcgggagggt
     4681 gaggccacgc ttgttcgcca tccgccggag cttctcgatg gccttccccg ggatctcgat
     4741 cttcttcggg cagaccttcc agcagtgctg gcaggtggtg cacataaaga ggccgtcccg
     4801 gatggcatcg ctgatccggt cgcaggagtc cctcgggtca atggcgaggc gcatctcctg
     4861 ccgcatcgtg agggggccgg cgaactcggc gacctccatg gccgggcaga ccgagacgca
     4921 ggccagg
//
LOCUS       DATGDI010000057         3727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_238171, whole genome shotgun sequence.
ACCESSION   DATGDI010000057 DATGDI010000000
VERSION     DATGDI010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238171"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2071
                     /gene="mgtA"
                     /locus_tag="VKO45_02155"
     CDS             <1..2071
                     /gene="mgtA"
                     /locus_tag="VKO45_02155"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HMA04709.1"
                     /translation="ADAIVLSAKDFFVNQSVLTGEPFPVERWPVTSPEGNGAEVRNRL
                     FLGTSVVSGTATAVITRTGGATQYGLIARNLSARPPETEMEKGLKRFGYLIMQLVFFL
                     VIFVFLINALYRHSVLESLLFAVALAVGVTPELLPMILSLNLSRGAMAMSGKGVIVKH
                     LASIQNLGSMDILCTDKTGTLTENRITLIRHVDSEGRDSEKVLLFSFLNSSHQTGLKS
                     PLDDAILAFRAIDTGRFRKVDEIPFDFVRKRISIIVGEDGRHLLISKGAPEEILKICT
                     SREVGDRIEDLGREAGDRILSLYRELSGEGFRALGVAYRTFTGDRSVYSTADEAGMVF
                     LGFVAFLDPPKESARESLELLNHAGVQLKILTGDNELVTRKTCTLLGYPEGGIVTGSD
                     IAAMDDDALSRVVERSTTFTRVTPDQKDRIILALKRNGHVVGYLGDGINDAPSLRTAD
                     IGISVDNAVDIARESSDIILLKKDLRVIHEGVLEGRRTFGNTMKYIMMGTSSNFGNMF
                     SVAAASLFLPFLPMLPIQILLNNLLYDLSQSAIPTDEVDPEYTLGPKRLDTGFIKRFI
                     LVFGPISSLFDILTFALLLLVFSATEHLFQTAWFIESLCTQVAIIFVIRTRRSPFFRS
                     RPSILLALSCTLVIAAAFILPYSPLGPAFGFVHLPASFYLILVALIAGYLALVEVVKR
                     WFYRNGD"
     gene            2114..2386
                     /locus_tag="VKO45_02160"
     CDS             2114..2386
                     /locus_tag="VKO45_02160"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HMA04710.1"
                     /translation="MPERLFTSRTRVKLLELFILHPEREVHVREICRITGTNINAVRR
                     ELANLEDLGLLRSRRAGNARFYTVNITFPIYQELVAILLKIGKGRE"
     gene            complement(2390..2758)
                     /locus_tag="VKO45_02165"
     CDS             complement(2390..2758)
                     /locus_tag="VKO45_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04711.1"
                     /translation="MSERFIFTKYRTSCYNCGKEVDQVIKAVRAQAQVACSNCGATRI
                     YIPRASEVGKPGLYTKIGCYDVWELRADAPCMNCCTEGPHDLIIGCSQFTTRCHHCGF
                     THFYKFNVEYIAQCPIPGKE"
     gene            complement(2836..3393)
                     /locus_tag="VKO45_02170"
     CDS             complement(2836..3393)
                     /locus_tag="VKO45_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HMA04712.1"
                     /translation="MAFRADLSSMGLDLVRKITAFVGRPEAAFREVKDEPVTPALIYF
                     VLLFLLYLVLSYIVFALGTLFLPIGPPGDLPVLFMPHILLQLLPLTLLPIYLGLLHGW
                     VYALGGRGGFSQTVKSVLYGLTPLLLLGYFSPLFLIAGIWSLGLITLGLRELRGMSLG
                     KAGLATLLPSIFLVVLIFILFLSAL"
     gene            complement(3463..>3727)
                     /locus_tag="VKO45_02175"
     CDS             complement(3463..>3727)
                     /locus_tag="VKO45_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HMA04713.1"
                     /translation="AEHDKKRKNYGLDVFKGAPIDMYKAGVVEPLRVKTQAVASAAEA
                     AVMILRIDDVIAASRVAGGPPGGPGGMPPGGMPPGMGGDMGDF"
BASE COUNT          732 a         1181 c         1116 g          698 t
ORIGIN      
        1 cgccgatgcc atcgtcctct ctgcgaagga cttcttcgtc aaccagtcag tcctcacggg
       61 agagcccttt ccggtcgaac gctggcccgt cacctcgccg gaggggaacg gggcagaggt
      121 gagaaaccgg ctcttcctcg ggacttcagt ggtgagcggt accgcaacgg cggtcatcac
      181 aaggaccggc ggtgccaccc agtacgggct catcgcccgg aacctctcgg cgaggccgcc
      241 cgagacggag atggagaaag ggctgaagag gttcgggtac ctcatcatgc agctcgtctt
      301 cttcctcgtc atcttcgtct tcctcatcaa tgccctgtac cggcacagcg tgctcgagtc
      361 cctcctcttc gccgtggccc tcgccgtcgg ggtcacgccc gagctcctcc ccatgatcct
      421 ctccctcaac ctctcccggg gggccatggc catgtcgggg aagggggtca tcgtgaagca
      481 cctggcctcc atccagaacc tcgggtccat ggacatcctc tgcaccgaca agaccgggac
      541 cctcaccgag aaccggatca ccctcatcag gcacgtggac agcgagggac gggacagcga
      601 gaaggttctc ctcttctcct tcctcaacag ctcgcaccag accgggctga agagccccct
      661 tgacgacgcg atccttgcat tccgggccat cgacacggga aggttccgga aggtggatga
      721 gatccccttc gacttcgtga gaaaaaggat ctccatcatc gtcggggaag atggccgaca
      781 cctcctcatc tccaagggag cccccgagga gatcctgaag atatgcacct cccgggaggt
      841 gggggaccgg atcgaggatc tgggccggga ggccggggac aggatcctct ccctctaccg
      901 cgaactgagc ggcgagggct tccgggccct cggggtcgcc taccgcacct tcacggggga
      961 ccgatccgtg tacagtacgg cggatgaggc cggcatggtc ttcctgggct tcgtcgcctt
     1021 cctcgatccg cccaaggaga gcgcccgtga atccctggag ctcctgaatc atgccggggt
     1081 ccagctgaag atcctgaccg gtgacaacga gctcgtgacc cggaagacct gcaccctcct
     1141 cggctacccg gaggggggaa tcgtcaccgg gtccgatatc gcggccatgg acgacgacgc
     1201 tctctcccga gtcgtggagc ggtcgaccac cttcaccagg gttaccccgg atcagaagga
     1261 caggatcatc ctcgcgctca agaggaacgg gcatgtggtc gggtacctgg gggacgggat
     1321 caacgatgcc ccctccctcc ggaccgcgga catcggcatc tccgtcgaca atgccgtgga
     1381 catcgcgagg gaatcctcgg acatcatcct actgaaaaaa gatctccggg tcatccatga
     1441 gggggtgctc gaggggaggc gcaccttcgg gaacaccatg aagtacatca tgatgggcac
     1501 ctcctccaac ttcggcaaca tgttcagtgt cgccgcggcc tccctcttcc tccccttcct
     1561 ccccatgctc cccatccaga tcctcctcaa caacctcctc tacgacctct cccagtcggc
     1621 catccccacc gacgaggtcg acccggagta caccctcggg ccgaagaggc tcgacaccgg
     1681 gttcatcaag aggtttatcc tcgtcttcgg gcccatcagc tccctcttcg atatcctcac
     1741 cttcgcgctc ctgctcctcg tattctcggc gaccgagcat ctcttccaga ccgcctggtt
     1801 tatcgagtcc ctctgcaccc aggtggccat catcttcgtc atccgcaccc ggcgctctcc
     1861 cttcttcagg agccggccga gcatcctcct cgctctgagc tgcaccctcg tgatcgcggc
     1921 ggccttcatc ctgccctaca gtccccttgg cccggccttc ggattcgtgc acctgcccgc
     1981 cagcttctat ctcatcctcg tagccctcat cgccggctac ctcgccctcg tcgaggtggt
     2041 gaagcggtgg ttttaccgga atggagattg aataaacttt atgttcaatt gaatactata
     2101 attatccata catatgcccg aacgcctctt cacttcccgt acccgggtga agctcctgga
     2161 actcttcatc ctccacccgg agcgggaagt ccatgtccgg gagatctgcc ggatcacggg
     2221 gacgaatatc aatgcggtgc ggcgggagct cgcgaacctg gaggatcttg gcctcctgag
     2281 aagccgccgg gcggggaatg cccggttcta cacggtgaac atcacgtttc cgatctacca
     2341 ggaactggtg gctattctcc taaaaatcgg aaagggccgg gaatgagttc tactccttcc
     2401 ccggtatcgg gcactgcgcg atgtattcca cgttgaactt gtagaagtgg gtgaagccac
     2461 agtggtggca ccgggtggtg aactggctgc acccgatgat caggtcgtgg gggccctccg
     2521 tgcagcagtt catgcacggg gcatcggccc tgagctccca gacgtcgtag cagccgatct
     2581 tcgtgtagag gccgggcttc cccacctccg aggcccgggg gatgtagatc cgggtcgccc
     2641 cgcagttgga gcaggccacc tgcgcctggg cccgcaccgc ctttatcacc tggtccacct
     2701 ccttcccgca gttgtagcag gaggtgcggt acttcgtgaa gatgaaccgc tcgctcatgt
     2761 tccttactgg tgccacccgg ggataaaaat ggtgcggata acagaaagat gttttacccc
     2821 gcgtgagggg ggctcctaaa gagccgaaag gaacaggatg aagataagaa ccacgagaaa
     2881 gatggaaggg agcagcgtcg caagacccgc cttccctagg gacatccccc gtaattccct
     2941 taatccgagg gtgatgaggc caaggctcca gatgccggcg atgaggaaaa ggggggagaa
     3001 atatcccagg agaaggaggg gggtgaggcc gtagaggacg gacttcaccg tctgtgagaa
     3061 ccctcccctt cccccgaggg cgtacaccca tccgtggagc agtccgaggt aaatggggag
     3121 aagggtcagg gggagcaact ggagtaggat atggggcatg aaaagcaccg gcagatcccc
     3181 gggagggcct atgggcagga acagagtccc cagggcgaac acgatgtaac tcaggacgag
     3241 ataaaggaga aaaaggagca cgaagtagat cagggcgggg gtaacaggct cgtcttttac
     3301 ctcacggaat gcggcctcag gcctcccgac aaaggccgtg attttcctga cgaggtccag
     3361 gcccatggag gagagatcgg ccctgaaggc cataacgctt cccggggagg tctggaaacc
     3421 ggttggagaa agatactaaa aagtgagaat ggtgtggggg gcttagaagt cgcccatgtc
     3481 gccgcccatg ccggggggca ttccgccggg aggcatgccg cctggcccac cggggggtcc
     3541 gcctgcgacg cgtgaggcgg cgatgacgtc gtcgatccgg aggatcatga ccgcggcctc
     3601 ggctgccgag gcgacggcct gggtcttcac cctgaggggc tcgacgacgc ccgctttgta
     3661 catgtcgatg ggtgcgccct tgaagacatc gaggccgtag ttcttcctct tcttgtcatg
     3721 ctctgcc
//
LOCUS       DATGDI010000058         1811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_180062, whole genome shotgun sequence.
ACCESSION   DATGDI010000058 DATGDI010000000
VERSION     DATGDI010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180062"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            554..>1811
                     /locus_tag="VKO45_02180"
     CDS             554..>1811
                     /locus_tag="VKO45_02180"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HMA04714.1"
                     /translation="MLAVLGIVLLLPMASGVETPAGIPDTDSDGVPDPWDICPVDTSL
                     PEVTVPSEGLNTNRFALVDGDAVFDTRAPKGKGPGRIYSLDGSIGSSTCGCSCEQILA
                     FSDGKKEGEFKFGCSIGIMDGFVRTRCEVDADGDGFRISDGDCDDSDPLRNPEATEEC
                     NGVDDDCDGLVPPDEADADGDGYRGCDGDCDDADPSIHPGATETCNGIDDDCDGQIDE
                     GAALTYYLDADGDGYGGMETVQACGAPTGYVETPGDCDDANPSVYPGAVELCDGLDTN
                     CDGKLSAGEADADGDGFRVCDGDCDDTNSAVHPGASEICDGLDNDCDGEVDEGVRLSF
                     YRDADGDGYGSRETLQDCSAPTGYAAVSGDCDDLNPAIYPGAAEVCDGKDNDCDVIVD
                     EGFDQDADRFTSCGGDCDDNDPEVYPG"
BASE COUNT          360 a          508 c          599 g          344 t
ORIGIN      
        1 ctccgaggta aatggccagg tggatcccgt agatgaaaaa gttggcaacg gggaggtaga
       61 ccataggtgt agcatgcccc tgtacccatt taaccctctc gatattatcc tgatcaggga
      121 ctgtggtcct tgttcagcct cggaaagacg ggttttcgtc ctgagacccc ccgattcccc
      181 gacgagcggt gacggccatc attgtcccga ttgtcggaga gctgccctga atgggagagc
      241 gttcaggtcc ttttcccgat cgtgaaattt cgataagttt ataattaaat atgtatattg
      301 aaccacatat cccccggtga tcgaagggca gagatggggt tcgaccgccg ggcgagtctg
      361 ccagcggcga tcccgatctc ccctgtcacg cgggtgtgcg cgagactgca cacagggcac
      421 gcgcatatcc acctgttttt ttccagaagg aaggtcctga ccgtccggac cggcgaggct
      481 cgacatcgat gaggaccgga tgggtggccg gaataagagg tattccatga gatcctttcg
      541 aaaacttctc ctggtgcttg ctgtcctggg tatcgtactg ctgctgccga tggcctccgg
      601 ggtggagaca cctgcaggaa tccccgacac ggattccgac ggtgttcccg atccgtggga
      661 tatctgccct gttgacacgt ccctccctga ggtaacggtg ccctccgaag gattgaacac
      721 gaacaggttt gccctcgtag atggcgatgc agttttcgac acccgggccc cgaagggaaa
      781 gggcccgggc cggatctaca gcttggacgg gagcatcggt tcatcgacct gcggctgctc
      841 ctgcgagcag atcctcgctt tttccgacgg gaaaaaagag ggcgagttca agttcgggtg
      901 cagcatcggg atcatggacg ggttcgtccg aacccggtgt gaggtggacg cggacgggga
      961 cgggttccgg ataagcgacg gggattgcga cgatagcgat cccctccgga atcccgaagc
     1021 caccgaggaa tgtaacgggg tcgatgacga ctgcgacgga ctcgtgcccc cggacgaggc
     1081 tgatgccgat ggtgacgggt accgaggctg cgacggcgac tgcgatgacg ccgatccttc
     1141 gatccatccc ggagcaaccg agacctgcaa cgggattgac gatgactgcg atggccagat
     1201 cgacgaggga gcggccctta cctactacct tgatgcggat ggtgacggtt atggtggcat
     1261 ggaaaccgta caggcctgtg gcgccccgac cgggtacgtg gaaacgccgg gggattgcga
     1321 tgacgcgaat ccgtcggtgt accccggggc cgtcgaactc tgcgacggcc tggataccaa
     1381 ctgtgacggg aagctctccg ccggagaggc ggacgccgat ggcgacggat tccgtgtctg
     1441 cgacggggac tgcgatgaca cgaattccgc ggtccacccg ggggcctcgg agatctgcga
     1501 cgggctggac aacgactgcg acggggaggt cgacgaaggt gtccggctct ccttctaccg
     1561 ggatgccgac ggggacggat atggcagccg ggaaaccctc caggactgtt cggcccccac
     1621 cgggtacgcg gcggtttcgg gcgattgcga tgatttaaac ccggcgatat accccggggc
     1681 tgccgaggtt tgcgacggga aggacaacga ctgcgacgtc atcgttgacg agggcttcga
     1741 ccaggacgct gacaggttca cgtcgtgcgg cggggactgc gacgacaacg accccgaggt
     1801 ctatcccggg t
//
LOCUS       DATGDI010000059         1554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_238310, whole genome shotgun sequence.
ACCESSION   DATGDI010000059 DATGDI010000000
VERSION     DATGDI010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238310"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VKO45_02185"
     CDS             <1..557
                     /locus_tag="VKO45_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04715.1"
                     /translation="APFLPPDLGEEIEALARISQGMDLPTDDPLGTGGLISDALRIAQ
                     VMVQGGGRFPDQAVPELDLPALLGEVLDAALLGLDSFADSGCTGLPADFRLAFRELGL
                     AIGLSSVDPLERLIHGSPAMFGPDSSLQVRAEALRSYFPLGETITRFWADGKNQESAG
                     WKEHREINMVMLATALAPGGFLDI"
     gene            complement(592..>1554)
                     /locus_tag="VKO45_02190"
     CDS             complement(592..>1554)
                     /locus_tag="VKO45_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HMA04716.1"
                     /translation="VFVLAHRLPVSALFDLASDPVCIIDQRRVLTFANTSFFSFFGSS
                     EKDALHRGLDEIGFDVSVIPQPGEFTRDASARKEGIREGEFRKGGTGYQFRIRMLPVT
                     FEDGMKGTAFLFDDITREKKHLENLEFLARTSAELADMGDDENIYQYIADRVAELEPR
                     AHVNVNSVDPERMITIVRATSSADLQFLREFNDSFADLGDFSEMTFDMNRIPEGIQVL
                     MKGSIMEGPRSLYEISWHLLPEAACSEIQEKFRLDRNFVMGCSCKGGLYGHVALRFRK
                     GDEMENKPTVEAFVRQAGVALQRRYLREKLRRIEGREETRRQTR"
BASE COUNT          294 a          499 c          437 g          324 t
ORIGIN      
        1 ccgccccatt tcttcccccg gatctcgggg aggagatcga agcacttgca cggatctccc
       61 agggcatgga ccttcccacc gacgatcctc tcggcaccgg cggcctgatc tccgatgccc
      121 tgcgcatcgc ccaggtgatg gtacagggcg ggggaagatt cccggaccag gccgtgccgg
      181 aactggatct gccggccctg ctcggcgaag tcctcgacgc cgcccttctc ggcctggatt
      241 ccttcgccga cagcgggtgc acgggacttc ccgccgattt ccggcttgcc ttccgggaac
      301 tcggcctcgc catagggctc tccagcgtgg atcccctgga gcggctcatc cacgggagcc
      361 cggcgatgtt cggccccgac agctccctgc aggtccgggc ggaggccctc cgatcctatt
      421 ttcccctcgg agagaccatc acgaggttct gggccgacgg gaaaaaccag gaatccgccg
      481 gctggaagga gcaccgggag atcaacatgg tgatgctcgc aacggccctc gcccccgggg
      541 gattcctgga tatataaaag gagggtgtcc tatccaggac cgccggttca cttaccttgt
      601 ctggcgccgg gtttcttccc tcccctctat ccttctcagt ttctcccgga ggtacctcct
      661 ctggagagca acccctgcct gcctcacgaa ggcttcgacg gtcggcttgt tctccatctc
      721 atcgcccttt ctgaatctca aggccacgtg gccgtacagt cctcccttgc atgaacagcc
      781 catcacgaaa tttctgtcaa ggcggaactt ctcctggatc tcgctacatg cggcctcagg
      841 aaggagatgc cagctgattt cgtataaact tctcggacct tccatgatgg aacccttcat
      901 gagaacctgg attccctcgg gaatcctgtt catgtcaaag gtcatttcgg agaaatcccc
      961 cagatcggca aagctgtcgt tgaactccct gagaaactgg agatccgctg acgaggtcgc
     1021 acgcacgatg gttatcattc tctcgggatc aaccgaattt acattgacgt gggcccgggg
     1081 ctcgagctcc gcgacccggt cggcgatgta ctggtagatg ttctcatcat ctcccatgtc
     1141 ggcgagctcg gccgaggtcc gggcgaggaa ctcaaggttt tccaggtgtt ttttctccct
     1201 cgtgatgtca tcaaagagaa acgcggtccc cttcatcccg tcctcgaagg tgacgggcag
     1261 catcctgatc cggaactgat accctgtccc cccctttcgg aactcccctt cgcgtatccc
     1321 ttctttccgg gcggaggcgt ccctcgtaaa ttcccccggc tgagggataa cggagacatc
     1381 aaacccgatc tcgtccagcc cacggtgaag ggcatccttc tccgaggatc cgaagaagga
     1441 gaagaagctc gtgttggcga aggtgaggac gcggcgctgg tcgatgatac agacgggatc
     1501 cgatgccagg tcgaagaggg ctgaaaccgg taaccggtgg gcgagaacaa agac
//
LOCUS       DATGDI010000060        22891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_63792, whole genome shotgun sequence.
ACCESSION   DATGDI010000060 DATGDI010000000
VERSION     DATGDI010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 22891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22891
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63792"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..345)
                     /locus_tag="VKO45_02195"
     CDS             complement(85..345)
                     /locus_tag="VKO45_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMA04717.1"
                     /translation="MGTAVLCFSQEGCMGCSEQAPINREMEKALRIRIEEVDVVKNPR
                     FVAQYHLRVTPTILILENGEERKRFEGLVHREELEAALRDYL"
     gene            complement(386..1075)
                     /locus_tag="VKO45_02200"
     CDS             complement(386..1075)
                     /locus_tag="VKO45_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04718.1"
                     /translation="MKREKKTESFPWKKVFVIAVGVLFVAMMVLSAMGMSWLQSFRSV
                     TANDTVTIGFTLRDAAGQPVLTTDQNVYQTAITGGYPVFLTQPLAVRAGHIGNPGYTG
                     VVAYNYYVSRSGMTEKFGILGQELDEIDAGVLGMKKGETKTIHFSFTVPPVSMKRSEF
                     MAMGGNFTQTSVGDLIPMGYSETPIVSGIVNESATNAVLRMATVVNKTADAIQIQDAY
                     PSADVTVEEIG"
     gene            1279..1764
                     /locus_tag="VKO45_02205"
     CDS             1279..1764
                     /locus_tag="VKO45_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMA04719.1"
                     /translation="MAWRRKYLFGPFWEEFDDFMADMEGQFNEMLEELASSKSLPGRE
                     MRARMLPAIRGEFRVDVKVHEDEVVVAADLPGVEKDDVSLSLLDPRTLRIAFTREQET
                     KEETEGYFVRERTYGSMDRVVKLPEDVTEEGTTATFKNGVLEVRLKKLVAETGKKISI
                     E"
     gene            1865..2650
                     /locus_tag="VKO45_02210"
     CDS             1865..2650
                     /locus_tag="VKO45_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA04720.1"
                     /translation="MDKKKVRDYMTYDVVTIDVHATVKDVIETIQTTKHDGFPVTNSG
                     EVVGYISARDLLFVHPSTPVERIMSTHLIVADPDMSINDAARVIFRSGIQKLPVVDET
                     NKLLGIISNADVIRSQIEHVSPDKVFKFIDTLRKLYGVEPKLHRDQVEIGSLLPTQTK
                     IYEDELEGRMYEIKKGLAEPLIVVKRPGRFILVDGHHRAIAAKRLGITHLEAYIIEID
                     KDIELGMERTARAMNLNSLDDIKVMDYARHPLVALTHRLVRHG"
     gene            complement(2659..3837)
                     /locus_tag="VKO45_02215"
     CDS             complement(2659..3837)
                     /locus_tag="VKO45_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HMA04721.1"
                     /translation="MKVCIMCGGEGTRLRPLTFERPKPCIPIVNIPSIAHLVSHLSNL
                     GFTEMVLTLGYLGEVIEKALGDGSLFGADIRYVREKTKLGTAGSVKNAQRHLEGGRFL
                     IVGGDHVTDVNLLEFYRSHALGKSIISIGLISIDDPSEFGIAEMDVNYHIKRFKEKPG
                     PGEIFSNLASMGMYVCDPEIFDHIPHGRKYDFARDLFPGLLSQGKTLRGWLARGNWTD
                     VGSPRSLRQAERWKLQEIPYMAISGDLHVQGAQIAGPVQFSGSVTLGPQSRIIGPVSI
                     GAGTTIEGNVLIGPYTSIGEGCTIRGHTKIFSSSIYNRVVVGRNCTISGSVVDTDSVL
                     GEECSVENDTIIGPRVVLRDGVVVHSGTRIWPEVTVEEKTVVREHILNPRFDTGIGGS
                     "
     gene            4026..4523
                     /locus_tag="VKO45_02220"
     CDS             4026..4523
                     /locus_tag="VKO45_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMA04722.1"
                     /translation="MPTFACTRCGKCCMNFGELIRIEREVDDTEFYCRFLITNEVFRA
                     RVMPDWREAFGDTAEVDANPAWCRFLRRAPEGQGFVCTIHDSNASICRDFRCCLMRIY
                     DGRGEQVGKIGGNRSLITEDEELRGFFREKVAGIDTSTVKEWLGRAGKVLTGAGYRVV
                     IYDEG"
     gene            4510..4773
                     /locus_tag="VKO45_02225"
     CDS             4510..4773
                     /locus_tag="VKO45_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04723.1"
                     /translation="MTKGRRTLRVVKGTVECVPGEGVTLDRCRFCAHSTRFLARGVWI
                     TSPARAYCVRSTAGKDVDLSAVEEVECDDLSGEGYRSITSIIS"
     gene            complement(4766..6286)
                     /locus_tag="VKO45_02230"
     CDS             complement(4766..6286)
                     /locus_tag="VKO45_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HMA04724.1"
                     /translation="MSLMVLGTASHAGKSMAVTALCRILHRRGIRVAPFKSQNMSLNS
                     FVTRDGREIGIAQAVQAFAAGVEPEAEMNPVLLKPKGEGVSQVVLMGRPYRDLPIRDY
                     YLETEFLLGEVLSAYRRLEERYGQLVVEGAGGAAEMNLYDRDIANILLARALRLPILL
                     IGDIERGGIFAQVYGTIQLLPEDIRPLVKGIIVNKFRGDPSLFSSGVRMLGEITGIPV
                     LGVIPYAEIPLPSEDSLSLADKRVGEGPVRIAVIRLPHISNFTDFEPLERLVPVDYVD
                     PGRNLSAYDCIIIPGTKNTVEDLAVLQDSGMAAEIRRARERGAPVIGICGGFQMLGKE
                     IHDQGIEGGHGVYPGLGLLSVITRFDRYEKTTRQVQIFANPVGPILSGMDEVTGYEIH
                     MGTTERGEEQEAFSGDGAVSSDGLVFGTYLHGLFQNETAVHALLSFLYGRKGLPFGGH
                     LMPGRTTLPIREEDSGVHGEPNTPGGSDPYDRLADLFEAHLDMEKILPFFSHNKAS"
     gene            6344..7984
                     /locus_tag="VKO45_02235"
     CDS             6344..7984
                     /locus_tag="VKO45_02235"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HMA04725.1"
                     /translation="MALTEAAAQLLKVRYMNAGETPEGVFPRVARGVDTPREGEFRRI
                     MEELLFLPNSPTLMNAGTSIGQLSACFVLPVEDSIEAIFRTMGHMAQIHKSGGGTGFS
                     FSRIRPKGDMVSGTAGIASGPVSFIEVFDKATEAVKQGGRRRGANMGILSFTHPDIRE
                     FVSSKKGGGLRNFNISVGLDATFFEAVRTGKDIPLANPRDGSVWREIPARDLWSEIAE
                     NAWATGDPGVLFMDEINRLSTLPGLGPIEATNPCGEQPLLPYESCNLGSINLSKCIQR
                     RELDEGMLADLARTGVDFLDAIIDVNRFPIPEIQEMNLATRKIGLGIMGFADALIKLG
                     IPYESDEALRFAGRVMALIQKAGHEKSRELGSEKGSFPAIGKSTFTGEMRNSTVTTIA
                     PTGSLHILADTSSGIEPLFALAFQRQMAGRTVNMVNELFVQAVSKRPGSADLIHRALV
                     EGSAQNLPIPDEMKDLFRTAPEISPEHHVRMQAAFQKHVDNAVSKTVNLPEDATAGDI
                     SRIYELARSLGCKGITVYRYNSKPDQVLSRGCEVCRVD"
     gene            8031..8336
                     /gene="yciH"
                     /locus_tag="VKO45_02240"
     CDS             8031..8336
                     /gene="yciH"
                     /locus_tag="VKO45_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HMA04726.1"
                     /translation="MNGGICPTCGLPKELCICEEVAKEQQRITVKINKRRYGKEVTVI
                     EGIDPADIDLGDLTKFLKGKLACGGTVKGSSIELQGNHLERVKELLAQKGFNLDNIS"
     gene            complement(8779..9813)
                     /locus_tag="VKO45_02245"
     CDS             complement(8779..9813)
                     /locus_tag="VKO45_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HMA04727.1"
                     /translation="MAFQDILNNFLNRSSYRPENLPTPVLDDYEKSQTEITDKIATQK
                     KTREGFGKFLKHPLAFVKEHPLNVLYVSGPIAAIVFVAGLVLIVTNYGILSLLQDTMI
                     DDVIVWAVLIAVIPLALLQYTETKRVYSLEESLPNFFRDVAGMNDSGMTLPNAIHNVA
                     AGEYGALTAYIRRLNAEMSWSVPFVEALIRFGERVGTPLAARSVDLIAKASRAGGDVS
                     EVLRAAANDTYEFFNLKNERKNNMLIYMVIVLVSFFVFLFVIAILESTFLTTMAEAGA
                     KVATTGAKGFMSKVNIEFYKRLFSHAAMIQGFFSGLVAGQMGEGDAVAGLKYSAIMLI
                     IAWVTFRFFI"
     gene            complement(9815..10738)
                     /locus_tag="VKO45_02250"
     CDS             complement(9815..10738)
                     /locus_tag="VKO45_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HMA04728.1"
                     /translation="MNSYQRICFKLMGERLKKKRGDYMTLRNNLMRARITVPFEAYLA
                     SAYVTSFFVGISAAVVLGLFSYLLRIPEMISYKGTLPDFLVELSQYRLIFGTVAAIVL
                     SLLIFGGLTYMVFLVYPSIHAGERRRNIDATLPYAINYITAMSTAGITPAEVFKLLGE
                     SPIYGESAAEARYIVREIEIFGKDLTDAIRVVAGLTPSDRMKEFFQGAVAAISAGTNL
                     TDYYRNKAVQYAVENRQQQKIFLETLGLIAESYVTALVAGTLFLIILQSIMSIISGEV
                     SPMFLYVVIYLIVPFGSVMFVILISSMTPEV"
     gene            complement(10735..14016)
                     /locus_tag="VKO45_02255"
     CDS             complement(10735..14016)
                     /locus_tag="VKO45_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HMA04729.1"
                     /translation="MTSQEPDKKPELDAMMKRLGLLGIENREPGPGGQGAEKKKGKAA
                     EPQSSPPLSPGRAQADELKDFLKHLETLEKDGVKQVEGLGPESDSVMAKLEKLDLASL
                     ETEARAPAPAQPSPRAGPEPAQARPQAPRKDDDFVALIRGIEEVKRDLTSEGGGKQAV
                     RREIKAEKVDTTVEKRRAPPEAEPQAKDADYASILDRLDKLDLANVGGETKTDIGEIT
                     AWKKDIRREPKGSAPAPEEAVSPQPPQAVQGGDTEAILKRLEGLSPAEPKAPAARPAP
                     AQKEAAPRVTVKAAHSGQEIDPEVILKKLEGLTPEEARAVAQKVALAAREAAPPRATK
                     PVQPAQGVDAESILRRLGEVPSQGRAPPRKEETAEPAPRATAAPAERRAAGRSKWEAV
                     APPAEEQKAKAAQAPDQGTGKDGSAKDVTSVIDNIFKVHKGAPVGAPSASEDVDASLK
                     QIIEGEGTPADEGAGEKEEVHAKKTRWSEGEMEEGQEAVVSVDEIKDISNLILPKGAT
                     FAIDEVKLHEHTAVFNAKGSVIPSELAENWQSQLPSGVIKDIVIGKTEEAKPKGLFGK
                     LNIMKVIRKEVEEYNPRIHGPLVDLDFRPAPNIEEVEVYPVNEPYAFIRVLHDNASHE
                     YTYTVLEPVLNQAEKDLVVELKQRLFETLEVNTKDLTKEAAHKAIRGAVDIVLSDYGI
                     SLTPVAREKILYNIEKEFLGDGLVDPVMHDKYIEDISCDGMNVPIFVYHTSYENTKTN
                     LIYKEAEDLDSFVTKMAQRAGKHISIAEPILDATMPDGSRIQMTLGSEVTAHGSTFTI
                     RKFREEPITPTDLIEWSTFSPLSVAYLWLAVEAGKSAIFAGGTASGKTTSLNAISLFI
                     PPMAKIVSLEDTRELKLPHPNWIPSITRDSFDTNGKGEIDMYELLRAALRQRPEYLIV
                     GEIRGKEALTLFQAMSTGHVTYATMHADSVASVVHRIENPPLSVPRNMLSALNLVCVQ
                     AQARIGGQRIRRSKQIIEVLDIDPRTNELITNEVFRWHPTTDEIRYSGKSYILEEIME
                     ERGWSEARMKEELKRRQELLEWMRLKMIRHYRDVSKMLISYFRDPEAVIELVRADLYA
                     EAKPA"
     gene            complement(14019..14651)
                     /locus_tag="VKO45_02260"
     CDS             complement(14019..14651)
                     /locus_tag="VKO45_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04730.1"
                     /translation="MDAGTGEIIQIIDEVLSTSTYVASYSYPELMAYTGSHAVNGIGV
                     HDENDRKFYIVFQAGAPEGVIFSDSKGTLFGDKAAILLTGQEPFELFDVSPSIVDALA
                     SRCRIFDKSHVKKRLDDDLPSFGGTHRSPGILCLTLAREGVPQQGIRVSIKKGRQIIA
                     SDITTTDGKVCFKLLNGTYDCMAVDRAQEVYTFMVDFNDRYVESVIEIGG"
     gene            complement(14830..15618)
                     /gene="minD"
                     /locus_tag="VKO45_02265"
     CDS             complement(14830..15618)
                     /gene="minD"
                     /locus_tag="VKO45_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATPase MinD"
                     /protein_id="HMA04731.1"
                     /translation="MFRAYTIASGKGGTGKTTVTVNLGTSLAQLGKETFILDADIGMA
                     NLGLVLGLESVPVTLHEVLAGKASVDDAIYEGPNGLKVVPSGISLQGFQNADPEKMKD
                     VMRDLIGRSDFLLIDAPAGISKDGVVALAIADEVILVVNPELSSMADALKTKILTEMV
                     GGKVYGAILNRAGMENTELRRHSVEDVLGVRVIDMIPEDANVRRAAAYKTPVVIKYPT
                     SGASRAFRRIAADLAGSEYKEEPVKVQEGFVDRLARTLFGGATR"
     gene            complement(15706..16386)
                     /locus_tag="VKO45_02270"
     CDS             complement(15706..16386)
                     /locus_tag="VKO45_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04732.1"
                     /translation="MQIPPSSREEVMKAIPLRTLLSELEKRQFTGYCAMPIAGAGALL
                     VMEEGRYILAEYRGIRGRDALESIRLIGNPLVDVILAPLPRNTLTEMRGTNEPFLVDP
                     PRPAGAPGAKNQPSPTVIRPVSIGGGTGRSVKVVTVKTGRREEARQPAGGQRAPAPAG
                     RPGAAKGPTGASLDRKGLEHLKSMKDSFKNDAADLLSELNLEHLVSKRSKSGQGNEGE
                     GKENSGTG"
     gene            complement(16386..18260)
                     /locus_tag="VKO45_02275"
     CDS             complement(16386..18260)
                     /locus_tag="VKO45_02275"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HMA04733.1"
                     /translation="MSTAPIPTGKIRGSLSDVTHKLQNFVGAVKVTTSSGKGGILVNM
                     GRPVAAYLAHGGVLSRGKDSLAILEKENLAEFEIIRYDEEQFREAMIICENRGLLLEG
                     EKVPSAGEPAQVREAKPREEENVLSAMMKRFAAPKVGKPEPVRPVPAEPVKPAPGIVK
                     EVPLPPSQPPVAAEKKITAPEERETLPSPSPSPSPSPVAETELSSIIKRLRETGRIEK
                     APAPPVSAAGEAPPKPGVFGAGAAAGGAGPAAVEEPGPAVPGAGIAGIPEGRAPEERV
                     RVDLGKMMKRIKRLDITSIRRELQEGEEELGPPGGSPGAVSGQPEETVTLSPAPAGET
                     TPEPEAKGRSDIDELKAFIKRLEVLDEPREPLPPVKDALPKIGAKPLAAESPALPLKR
                     APEPVPEKEGVREPVSAPAEQAEPVPKPPEEKVIRPVEPERKARIASLLRRSGLVKPP
                     AEKEPEEPAPAIMEMPAIPEEKVKPPSPAPAPAEEVELRIPKPQRKRVEITRVPKKPE
                     RPLKEPPLIDEKMIQRILKQPGVIAVSTFYEGFAVQSAGRADFEQVAANAEDLFRAGK
                     KIAREIDIGPLNQIILETGKGKLIIAPHGDLNVCIFTDPDANLGLIRVALKGLMAEKD
                     "
     gene            complement(18268..18615)
                     /locus_tag="VKO45_02280"
     CDS             complement(18268..18615)
                     /locus_tag="VKO45_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HMA04734.1"
                     /translation="MLNQILAEFLKLDGVFAAVVVGRDGMVIESAVSGKVDTDALGAM
                     ASTGMGTAEAMGNELGKGELNQMLVELEKGPILLSPLSKDELIAIVSDNTGNVGRIRY
                     ELKKNKERIIAAL"
     gene            complement(18654..20033)
                     /locus_tag="VKO45_02285"
     CDS             complement(18654..20033)
                     /locus_tag="VKO45_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HMA04735.1"
                     /translation="MTEIRISPLTRLEGHAQVRITLDAKNQVERAHFNVMELRGFEKF
                     LIGAAVEEAPRITPRICGICPTSHHLASVKAVDAILGAEPPATGKKLRELLNHAQYIH
                     SHAIHFFMLAAPDFLVGHDAPAADRSVIGIAKKSPALAKKAIEVRKLGQRITEAVGGK
                     PIHPSNAVPGGMSRALDETARGNLLGMAKAGLAFAKEGWELARGMMDGTDLSLGTVET
                     GFLAMTRDKKYSPYEGSVEMIGKDGATLGGFTGADYAKHIGEFSEDWSYLKFTRLSDG
                     TFYRVGPLARLNICTSMGTPLADAALKEYRAKFGGTVQAPLAYNLARYVEFLASCERA
                     VELLEDPEITGANIRSPVTGVKAKRGVGIIEAPRGTLIHDYTVNDQGFIERCNLIVAT
                     CQNNYAIDRSVEAVARTFVKDGTLPEAAANRIEMVIRAYDPCISCATHAIGKGSWPIE
                     IVRSGVPLR"
     gene            complement(20030..20947)
                     /locus_tag="VKO45_02290"
     CDS             complement(20030..20947)
                     /locus_tag="VKO45_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-nonreducing hydrogenase"
                     /protein_id="HMA04736.1"
                     /translation="MKIAIEELASCSGCTIAVLDLHEALLDVLGKAEIVYAPVIMDVK
                     EPPEGIDIAFVTGAVRNEENEERLKKIRKRAKTVVALGTCACYGGISGLSMLFNREDM
                     FNHVFRGVETVKPDGVIPKDVPPFLYRAFAVGDLAKVDYYITGCPPKEMYLKKILPAL
                     VSGEPLSLSKKSVCSECDRIMGAHEKWQLKRRYAGVPDREHCLLGQGYLCLGAVTFGR
                     CGASCPHSNVPCHGCNGPSLDILREPCRDIYNMMIRRIADLTDKPEAEIEKEIYDVAH
                     TFYPFTIGSLIMEDKGISKIRDLIKERNV"
     gene            complement(20959..21522)
                     /locus_tag="VKO45_02295"
     CDS             complement(20959..21522)
                     /locus_tag="VKO45_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrocarbon binding protein (contains V4R
                     domain)"
                     /protein_id="HMA04737.1"
                     /translation="MTDGAIEKMKGAFSTDLVYRSEDVPLTCIPHPDELEDTLHGLMK
                     LNGLIIRSLEEVAGRGANAVTYRAGRKYGHEVAKYFRKMENIEEALKELSDILRGQFT
                     IEVWKPADKPSYIIEENGSTFIYLVFHDCIVRQTLRRNGQVQGGPLCQTLYGYVVGAV
                     EEITGRKAKLEIVHTGPNACLKKLILQ"
     gene            complement(21656..21901)
                     /locus_tag="VKO45_02300"
     CDS             complement(21656..21901)
                     /locus_tag="VKO45_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA04738.1"
                     /translation="MIAIKVDDSACNGCGLCVKDCPMHVYELRDDLSVPVHPENCMGC
                     LSCHEICPAQALEHRGIYPSRRHYIDIRVCEMLNRVI"
     gene            22028..>22891
                     /locus_tag="VKO45_02305"
     CDS             22028..>22891
                     /locus_tag="VKO45_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04739.1"
                     /translation="MIVPLVVASVAVLATLAYASILDVRERSVPVRVWYPLYLAGSAA
                     VIWYLFTSGSGWGTLSGYAALIVTLVYGIELEAEDGKIPAKVLLLALGATLLQGLAWG
                     FLWRELGIGAIAGFVLLAVFLWEGLELERRASLDEKTLSPASRQFLLWHLPAIVLLQA
                     AGWGALFLSGRGGTGDLVLVLVALYSLLFLLFAVTNLFGFADAIALIAIALFVPRFPF
                     QPILGYPPLDFLPFSALTNAVILNLVTPVGIAVWNAVKGNRAPFPYTFFGFPVRGDTI
                     EHTFGFVMEEFE"
BASE COUNT         3979 a         7215 c         7227 g         4470 t
ORIGIN      
        1 atgaagctcc ggaacgggaa ggtgcttcgc aaggaggggc agagggctcc cgcccgggcc
       61 gtgaaacgga tctacgggaa ccggttatag gtaatccctc agggccgcct cgagttcctc
      121 ccggtggaca agcccctcga agcgtttcct ctcctcaccg ttctcgagga tgaggatggt
      181 gggagtgacc cggaggtggt actgggcgac gaacctcgga ttcttcacca cgtcgacctc
      241 ctcgatccgg atgcgcaggg ccttctccat ctcacggttg atgggggcct gctcgctgca
      301 gcccatgcac ccctcctgcg aaaaacagag gacggcggtt cccatgggag aagggtggcg
      361 gcggggggag aaaaaagtat ggaatctacc cgatttcctc gaccgtgaca tcggctgagg
      421 gatacgcatc ctggatctgg atggcatccg ccgtcttgtt caccacggtg gccatcctga
      481 gcacggcgtt cgtcgctgat tcattcacta tgcccgaaac gatgggtgtc tcggagtagc
      541 ccatggggat caggtcgccc acgctcgtct gggtgaagtt tccccccatg gccatgaatt
      601 cagagcgttt catggagacg gggggaaccg tgaaactgaa gtggatggtt tttgtctcgc
      661 ccttcttcat gccgaggacc ccggcatcga tctcgtccag ttcctggccg aggatgccga
      721 acttctccgt catgccggag cggctcacgt agtaattgta cgcaactacg cccgtgtagc
      781 ccgggttacc gatgtgaccg gccctcaccg cgagaggctg ggtcaggaaa accggatacc
      841 cgccggtgat ggcggtctgg tacacgttct ggtccgtggt gagcaccggc tggccggcgg
      901 cgtccctgag ggtaaatccg atggttacgg tatcgttggc cgtcacgctc cggaaggact
      961 ggagccagct catccccatg gccgagagga ccatcatggc cacgaagaga accccgacgg
     1021 cgatgacaaa cacctttttc cagggaaaac tctccgtttt cttctccctt ttcatccgta
     1081 ctgagaaggt tccacggcga gataaaatct ttcatggaca ggaaacggca gaccgactcc
     1141 cgcatccgtc agacggtgag ggcaattcta aaaaattgag attcggggag acctatttat
     1201 actaccggta cgacctgtcc cttgcctgtg cccgaagcgg catggatgaa gcagtaaccc
     1261 caaggagagg aaacccgtat ggcatggaga cggaagtacc tgtttggacc gttctgggag
     1321 gaattcgatg atttcatggc cgacatggag ggccagttca acgagatgct cgaggagctc
     1381 gcgagctcga agtccctccc cgggcgggag atgcgggcga ggatgctccc cgcgatccgc
     1441 ggcgagttcc gggtggacgt gaaggtgcac gaggatgagg tcgtcgtggc cgcggacctc
     1501 ccgggcgtcg agaaggacga tgtcagcctc tcgctcctgg acccgagaac acttcgaatc
     1561 gcctttaccc gcgagcagga gaccaaggag gagaccgagg gctacttcgt ccgggagagg
     1621 acctacgggt ccatggaccg ggtggtgaag ctccccgagg acgtgacaga ggaggggacg
     1681 acggcgacct tcaagaacgg ggtcctcgag gtgaggctga agaaactcgt cgccgagacg
     1741 ggaaagaaga tttccatcga ataaaaccaa cccttttcac ctctggtgac ccagggccat
     1801 ttctctgaaa tgtccccttt ttcaccgatc aattaagtac ggagaacgtc ctagagctct
     1861 agtgatggac aagaaaaagg tcagggacta catgacctac gacgtcgtga ccatcgacgt
     1921 ccatgccacg gtgaaggacg tcatcgagac catccagacc acgaagcacg acggctttcc
     1981 cgtgaccaac agcggcgagg tggtgggcta catctccgcc cgggacctcc tgttcgtcca
     2041 cccatccacc cccgtggaac ggatcatgtc cacccacctg atcgtcgccg accccgatat
     2101 gtccatcaac gacgccgccc gggtcatctt ccggagcggg attcagaagc tcccggtggt
     2161 tgacgagacg aacaagctcc tcgggatcat ctcgaacgcg gacgtgatcc gatcccagat
     2221 cgagcacgtc tcgccggaca aggttttcaa gttcatcgac accctgcgga agctctacgg
     2281 ggtcgagccc aagctccacc gggaccaggt ggagatcggg agcctcctcc ccacccagac
     2341 aaagatctac gaggacgagc tggaggggag gatgtacgag atcaagaagg gcctcgccga
     2401 gcccctcatc gtggtgaagc gcccgggccg gttcatcctc gttgacgggc accaccgggc
     2461 catcgcggca aagaggctcg ggatcaccca cctcgaggcc tacatcatcg agatcgacaa
     2521 ggacatcgag ctcgggatgg agcggaccgc ccgggccatg aacctcaact ctctcgacga
     2581 tatcaaggtc atggattacg cccggcaccc cctcgtcgcc ctcacccacc gcctcgtccg
     2641 gcacgggtaa attaaaacct aggagccgcc gatcccggtg tcaaacctcg ggttgaggat
     2701 gtgctcccgg acgacggttt tctcctccac cgtcacctcg ggccagatgc gggtccccga
     2761 gtggaccacg accccgtccc ggagcaccac ccggggaccg ataatggtgt cgttctcgac
     2821 ggagcactcc tccccgagca ccgaatcggt atcgacgacc gacccgctga tggtgcagtt
     2881 cctcccgaca acgacccggt tgtagatgga tgacgagaag atcttcgtgt gcccgcggat
     2941 ggtgcaaccc tcgccgatgg aggtgtaggg gccgatgaga acgtttccct cgatggtggt
     3001 ccccgccccg atggagaccg ggccgatgat ccgggactgg gggccgagag tgaccgatcc
     3061 cgagaactgg accgggcccg cgatctgggc accctggaca tggaggtcgc ccgagatggc
     3121 catgtaggga atctcctgga gtttccagcg ctcggcctgc cggagggacc tcgggctccc
     3181 gacgtccgtc cagttgcccc gggcgagcca gcccctgagg gtctttccct gggagaggag
     3241 gcccgggaag aggtcccggg cgaagtcgta cttccgacca tgggggatgt ggtcaaagat
     3301 ctccgggtca cagacgtaca tgcccatgct cgcgaggttc gagaagatct cccccggccc
     3361 gggcttttcc ttgaacctct tgatgtggta gttcacgtcc atctcggcga tgccgaactc
     3421 gctcgggtcg tcgatggaga tgagcccgat ggagatgatg ctcttcccga gggcatggga
     3481 ccggtagaac tcgaggaggt tcacgtcggt gacgtggtct ccgccgacga tgaggaaccg
     3541 gcccccctcg aggtgccgct gggcgttctt caccgagcct gcggtcccga gcttggtctt
     3601 ttcccggacg taccggatgt ccgccccgaa gagggagccg tcgccgagag ccttctcgat
     3661 gacctccccc aagtacccca gggtgaggac catctcggtg aaccccagat tggaaaggtg
     3721 ggagaccagg tgggcgatgg acgggatatt gacaatggga atgcagggtt tcggccgctc
     3781 gaaggtgagg ggacggagcc gggtcccctc gcccccgcac atgatgcaga ccttcatcga
     3841 ggagtatctg ttcaggaggt aattaagggt gcatggtgga acctgccctt tggggcctcg
     3901 ggggatccgg gccgtgaggc tgatgcgatc cgggccgggg gaggcacaag gggatccagg
     3961 ccgcgtgagg cttgatggga tccgggccga acccttaata cgcctcccgg gatactctcc
     4021 gggggatgcc caccttcgcc tgtacccggt gcgggaaatg ctgcatgaac ttcggggagc
     4081 tcatccgcat cgagcgggag gtggatgata ccgagttcta ctgccggttc ctcatcacga
     4141 acgaggtctt ccgggcccgg gtcatgcccg attggaggga ggccttcggg gatacggccg
     4201 aggtggacgc gaacccggcc tggtgccggt tcctcaggcg ggcccccgaa gggcagggct
     4261 ttgtctgcac gatccatgac tcgaatgcat cgatctgccg ggacttccgg tgctgcctga
     4321 tgcggatcta cgacgggagg ggggagcagg tgggaaaaat cggcggcaac cggagcctca
     4381 tcaccgagga cgaggagctc agggggtttt tcagggagaa ggtggccggt atcgatacct
     4441 ccaccgtaaa ggaatggctc gggagggcag ggaaggtcct taccggggcc ggttaccggg
     4501 tggtcatcta tgacgaaggg taggcgcacc cttcgggttg tgaaggggac cgtagagtgc
     4561 gttcccggcg aaggagtaac gctggaccgc tgccggttct gcgcacactc cacccggttt
     4621 ctcgccaggg gagtctggat cacatcccct gcacgggctt actgcgtccg gagcacggcc
     4681 gggaaggacg tggacctctc ggcggttgaa gaggtggagt gcgacgatct ctccggcgag
     4741 gggtaccggt ccatcacgag tatcatcagc tagccttgtt gtgagaaaag aaggggagaa
     4801 tcttttccat atccaggtgg gcctcgaaga gatcggcaag ccggtcatag ggatctgacc
     4861 cccccggggt gtttggctct ccgtgaaccc cggaatcctc ctcgcggatg gggagggtcg
     4921 tcctccccgg catgaggtgt cccccgaagg ggaggccctt ccgcccgtag aggaaagaga
     4981 ggagggcatg cacggcggtc tcgttctgga acagcccgtg caggtaggtc ccgaagacga
     5041 gaccgtcgga gctaacggcc ccgtcccccg agaatgcctc ctgctcctcc ccccgctcgg
     5101 tcgtgcccat gtggatctcg tatcccgtca cctcgtccat gcccgagagg atggggccga
     5161 ccgggttcgc aaagatctgg acctgcctcg tcgtcttctc gtaccggtcg aagcgggtga
     5221 tcaccgagag gagaccgagg cccgggtaga ccccgtgccc tccctcgatc ccctggtcat
     5281 ggatctcctt cccgagcatc tggaagcccc cgcagatgcc gatgaccgga gccccccttt
     5341 ccctcgccct ccggatctcg gccgccatcc ccgaatcctg gagcacggcg agatcctcga
     5401 cggtattctt cgtgccgggg atgatgatac agtcatatgc cgagaggttc cggccggggt
     5461 caacatagtc cacgggcacg aggcgctcga ggggctcgaa gtcggtgaag ttggagatgt
     5521 ggggcaggcg gatgaccgcg atccgcaccg gcccctcgcc caccctcttg tcggcgaggg
     5581 agagggagtc ctcgctcggg agcgggatct ctgcgtacgg aatgacgccg aggacaggta
     5641 tccccgtgat ctccccgagc atccggacac ccgacgagaa aagggagggg tcccccctga
     5701 acttgttcac gatgatccct ttcacgaggg gacggatgtc ctcggggagg agctggatgg
     5761 tcccgtacac ctgggcgaag atcccgcccc gttcgatgtc cccgatgagg agaatgggga
     5821 gccggagggc ccgggcgagg aggatgtttg cgatgtcccg gtcatagagg ttcatctcgg
     5881 cggctccccc cgccccctcg acgaccagct ggccgtaccg ctcctccagc ctccggtacg
     5941 ccgagaggac ctccccgagg aggaactccg tctccaggta gtaatccctg atggggaggt
     6001 cgcggtacgg tctgcccatg aggaccacct gggacactcc ctcgcccttc ggtttgagca
     6061 gaacggggtt catctcggcc tccggctcca cgcctgcggc aaaggcctgg accgcctggg
     6121 cgatcccgat ctccctcccg tcccgggtca cgaaggagtt caggctcatg ttctgggact
     6181 tgaaaggggc cacccggatg ccccggcggt gaagaatccg gcagagggcg gtgaccgcca
     6241 tgctctttcc cgcgtgggat gcggtcccga gaaccatgag ggacatgtac gctgaaagat
     6301 gggcatggat ttttattaaa ggagatcctt aaccctcaac cgtatggcac tcaccgaggc
     6361 ggcggcgcag ctcctcaagg tcaggtatat gaacgccggc gagactcccg agggagtctt
     6421 tccccgggtg gccagggggg ttgacactcc cagggaaggg gaattccgcc ggatcatgga
     6481 ggagctcctc ttcctcccca actccccgac cctgatgaac gccggtacct ctatcggcca
     6541 gctctccgcc tgtttcgtcc ttcccgtgga ggactccatc gaggccatct tccggaccat
     6601 gggccacatg gcccagatcc acaagagcgg cggagggacg ggcttctcct tctcccggat
     6661 ccggccgaag ggggacatgg tgagcggcac cgcgggcatc gcgagcggtc ctgtctcctt
     6721 catcgaggtc ttcgacaagg ccacagaggc cgtgaagcag ggtggccggc gcaggggtgc
     6781 gaacatgggg attctctcct ttacccatcc ggacatccgg gagttcgtct cctcaaagaa
     6841 gggcggaggc ctccgcaact tcaacatctc cgtgggactg gatgccacct tcttcgaggc
     6901 ggtgaggaca ggaaaggata tccccctcgc gaacccccgg gacgggagcg tctggcggga
     6961 gatccccgca cgggacctct ggagtgaaat cgctgagaac gcctgggcaa cgggcgatcc
     7021 cggggttctc ttcatggatg agatcaaccg gctctccacc ctcccgggcc tcggccccat
     7081 cgaggcgacg aacccctgcg gcgagcagcc tctcctcccg tacgagagct gcaacctcgg
     7141 gtccatcaac ctctcgaaat gcatacagag gagggaactc gacgagggga tgctcgccga
     7201 tctcgcccgg accggggtcg acttcctgga tgccatcatc gacgttaacc ggttccccat
     7261 ccccgagatc caggagatga acctggccac ccggaagatc ggcctcggga tcatgggctt
     7321 cgccgacgcc ctcatcaagc tcggcatccc gtacgagtcc gacgaggccc tccgtttcgc
     7381 cgggcgggtc atggccctca tccagaaggc ggggcacgag aagtcgcggg agctcgggtc
     7441 agagaaggga tccttcccgg ccatcgggaa gagcaccttc accggcgaga tgcggaactc
     7501 cacggtgacc accatcgccc cgacgggctc cctccacatc ctcgctgaca cctcctccgg
     7561 gatcgagccc ctcttcgccc tcgccttcca gaggcagatg gcgggaagaa cggtgaacat
     7621 ggtgaacgag ctcttcgtcc aggcggtctc gaaacgcccg ggctcggcag atctcatcca
     7681 ccgggccctg gtggagggtt cggcacagaa cctccccatc cccgacgaga tgaaggacct
     7741 cttccggaca gccccggaga tctcccccga gcaccatgtc cggatgcagg cggccttcca
     7801 gaaacacgtg gacaacgccg tctcgaagac cgtgaacctc cccgaggacg caaccgccgg
     7861 ggacatctcg aggatctacg agctcgcacg ttccctcggg tgcaagggca tcacggtgta
     7921 ccggtacaac agcaagcccg accaggtcct ttcccggggg tgcgaggtct gccgggtgga
     7981 ctaaatttca tgccaagaaa taaataagga gaattttaac atagaaggta atgaatggtg
     8041 ggatttgtcc gacctgcggg cttccaaagg aattatgtat ctgtgaggag gtagcaaagg
     8101 aacagcaaag gatcaccgtg aagatcaaca agaggcgcta cgggaaggag gtcacggtca
     8161 tcgagggcat cgatcccgcg gacatcgacc tcggggacct gaccaagttc ctgaagggga
     8221 agctcgcctg cgggggaacg gtgaagggct cctccatcga gctccaggga aatcacctcg
     8281 agcgggtgaa ggagctcctc gcccagaagg gattcaacct ggataatatc agctgatttt
     8341 ttcggttttt ctgatttttc ttcttcggga tttgagagaa cccggtgaga aaataaagat
     8401 cttcaaacta acccctgatc cgaacttcat tgctgaccgg cggacgttcc tgggggactc
     8461 gctctcgctc gcccccgccg ctccggcgtc ccccgatttg cgatagctcc ctcattccgt
     8521 ttctcatatt acgtaattcc ttgaaccgcg gaccgggagc acataccggt aactgggggg
     8581 tgcccgtgcg gcgggggcgg cgtagcggat cgcgacttcg tcgctccagg tcgggttatg
     8641 ataaaccctc cctcccccga gagcacccca ataagaaact cagatgtacc ctgattgttc
     8701 cataacggaa tttaattcct cagtaaagtg actagaaagt ccaactcgcc gaaaaaagag
     8761 ggatcccgga ttccttcttc aaatgaagaa ccggaaggtc acccaggcga tgatgagcat
     8821 gatggccgag tacttgagcc ccgccaccgc atccccctca cccatctgcc ctgccacgag
     8881 gcccgagaag aacccctgga tcatggccgc gtgggagaag agccgcttgt agaattcgat
     8941 gttcaccttg gacatgaagc ccttggcccc tgtcgttgcg accttggccc ccgcctcggc
     9001 catggtggtg aggaaggtgg actcgaggat ggcgatgacg aagaggaaca cgaagaacga
     9061 gacgaggacg atgaccatgt agatgagcat gttgttcttc cgctcgttct tcaggttgaa
     9121 gaactcgtac gtgtcattcg cggcggcccg gaggacctcg gaaacgtccc ccccggcccg
     9181 ggaagccttg gcgataaggt ccacggaccg ggccgcgagg ggggtcccga cccgctctcc
     9241 gaaccggatc agggcctcga cgaagggaac cgaccaggac atttcggcgt tgagccgccg
     9301 gatatacgcg gtgagggccc cgtactcgcc ggcggcaacg ttgtggatgg cgttcgggag
     9361 ggtcatcccg ctgtcgttca tgcccgccac gtcccggaag aagttgggca gggactcctc
     9421 gagggagtac acccgcttcg tttccgtgta ctggaggagg gcgaggggga tcacggcgat
     9481 gaggaccgcc cagacgatga catcgtcgat catcgtatcc tggagcaggg aaagaatccc
     9541 gtagttcgtg acgatgagga cgagcccggc gacaaagacg atcgccgcga tgggtcccga
     9601 gacgtagagg acattgaggg gatgttcctt gacgaaggcg aggggatgct tcaggaactt
     9661 cccgaagccc tcccgggtct tcttctgggt cgcgatctta tcggtgatct ccgtctggga
     9721 cttctcgtag tcatccagga ccggcgtcgg caggttctcg gggcggtacg aggaccggtt
     9781 gaggaagttg ttcaggatat cctggaaggc catgtcacac ctccggcgtc atcgagctga
     9841 tgaggatgac gaacatgacg ctcccgaagg gaacgatgag gtagatgacc acgtagagga
     9901 acatggggct cacctcgccg ctgatgatgg acatgatgga ctggaggatg atgaggaaga
     9961 gggtgcccgc gacgagggcc gtcacgtacg actcggcgat gagccccagg gtctcgagga
    10021 atatcttctg ctgctgccgg ttctcaacag cgtactgcac ggccttgttg cggtaataat
    10081 cggtgagatt cgttcctgcc gagattgccg ctacggcccc ctggaaaaac tccttcatcc
    10141 ggtcgctggg ggtgaggccg gcgaccaccc tgatggcatc ggtgaggtcc ttcccgaaga
    10201 tctcgatctc cctcacgatg taccgtgcct ccgccgcact ctccccgtag atggggcttt
    10261 cgcccagaag cttgaagacc tcggccggcg tgattcccgc ggtggacatg gccgtgatgt
    10321 agttgatggc ataggggagg gtggcatcga tgttcctccg gcgctcgccg gcatggatgc
    10381 tcgggtagac gaggaagacc atgtaggtca gcccgccgaa gatgaggagg gagaggacga
    10441 tcgcggccac ggtcccgaag atgagcctgt actgggagag ctcgacgaga aagtcgggga
    10501 gggtcccctt gtagctgatc atctccggga tccggaggag gtaggagaag aggccgagga
    10561 ccaccgcggc gctgattccg acgaagaagg aggtgacata ggccgaggcc aggtacgcct
    10621 cgaagggcac ggtgatccgg gccctcatga ggttattcct gagcgtcatg tagtcgcccc
    10681 tcttcttctt cagccgttct cccatgagct tgaagcagat tcgctggtaa ctgttcatgc
    10741 cggcttcgcc tcggcgtaga ggtcggccct gacgagctcg atgaccgcct cggggtcacg
    10801 gaagtaggag atgagcatct tcgatacgtc ccggtagtgc cggatcatct tgaggcgcat
    10861 ccactcgagg agctcctgcc tgcgcttcag ctcctccttc atccgggcct cgctccagcc
    10921 ccgctcctcc atgatctcct cgaggatgta tgacttcccc gagtaccgga tctcgtcggt
    10981 cgtcgggtgc cagcggaaga cctcgttcgt gatgagctcg ttcgttcgcg ggtcgatgtc
    11041 gaggacctcg atgatctgct tggaccgacg gatcctctgc cccccgatcc tggcctgggc
    11101 ctgcacacag acgaggttta aggccgagag catgttcctc gggaccgaga ggggcgggtt
    11161 ctcgatacgg tggacgaccg aggctaccga gtcggcgtgc atggtcgcgt aggtgacatg
    11221 gcccgtggac atggcctgga agagggtgag ggcctccttg ccacggattt cacccacgat
    11281 gaggtactcg ggcctctgac gcagagcggc cctgaggagc tcgtacatgt cgatctcccc
    11341 cttgccgttc gtgtcaaagg agtcccgggt aatgctcggg atccagttcg ggtggggaag
    11401 cttcagctcc cgggtgtcct cgagggagac gatcttggcc atcgggggga tgaagaggga
    11461 aatggcattg agggaggtcg tcttccccga tgcggtcccg ccggcaaaga tggcggactt
    11521 cccggcctcc acggcgagcc agaggtaggc caccgagagg ggcgagaagg tggaccactc
    11581 gatgagatcc gtgggggtga tgggctcctc ccggaacttc cggatggtga aggtggagcc
    11641 gtgggccgtg acctcgctcc cgagggtcat ctggatacgg gagccgtcgg gcatcgtggc
    11701 gtcgaggatg ggctcggcga tggagatgtg cttccctgcc ctctgggcca tcttcgtgac
    11761 aaaggagtcc aggtcctcgg cctccttgta gataaggttc gtcttcgtgt tctcgtagct
    11821 cgtgtggtac acgaagatgg ggacgttcat gccatcgcag gaaatatcct cgatgtactt
    11881 gtcgtgcatg accgggtcga cgagaccgtc cccgaggaat tccttctcga tgttgtagag
    11941 gatcttctcg cgggcgaccg gcgtgaggga gatcccgtag tccgagagca cgatatccac
    12001 cgccccccgg atggccttgt gagccgcctc cttggtaagg tccttcgtgt ttacctcgag
    12061 ggtctcgaag agccgctgct tcagctccac aaccaggtcc ttctcggcct ggttgaggac
    12121 cggttcgagt accgtgtacg tgtactcatg agaggcattg tcatggagga cccggatgaa
    12181 ggcatagggc tcgttcaccg ggtacacctc cacctcctcg atgttgggtg cgggacggaa
    12241 atcgaggtcc acgaggggac cgtgaatccg cgggttgtac tcctcgacct ccttccggat
    12301 caccttcatg atgttcagct tgccgaagag gcccttcggc ttcgcctcct ccgtcttccc
    12361 gatgacgatg tccttgatga ccccggacgg gagctgggac tgccagttct cggcgagctc
    12421 gctcgggatg acgctcccct tcgcgttgaa gaccgccgtg tgctcgtgga gcttcacctc
    12481 gtcgatggca aaggtggccc ccttggggag gatcaggtta ctgatgtcct taatctcgtc
    12541 caccgagacg acggcctcct gcccctcctc catctcgccc tcggaccagc gggtcttctt
    12601 cgcatggacc tcctccttct ccccggcacc ttcatcggcg ggcgttccct caccctcgat
    12661 gatctgcttc agggatgcgt ccacgtcctc gctcgcagac ggggcaccca caggggcccc
    12721 cttgtggacc ttgaagatgt tgtcgatgac cgaggtgacg tccttcgcag agccgtcttt
    12781 tcccgtcccc tggtcggggg cctgggcggc cttcgccttc tgttcctcgg cggggggtgc
    12841 gacggcctcc cacttgctcc ttccggctgc ccttctctcg gcaggggccg ccgttgcccg
    12901 gggggcgggc tcggcggttt cctccttccg ggggggggcc ctcccctgcg agggtacctc
    12961 cccgaggcgc cggaggatcg attcggcatc cacaccctgg gcgggctgca cgggcttcgt
    13021 tgccctgggg ggtgcggcct ccctcgcggc gagggccacc ttctgggcaa cggcccgggc
    13081 ctcctccggg gtgagaccct cgagcttctt taaaatcacc tccgggtcga tctcctgccc
    13141 ggaatgggcg gccttgaccg tcacccgggg ggcggcctcc ttctgggccg gcgccggcct
    13201 cgcagccggg gccttcggct ctgcggggga gaggccctcg agccgcttca ggatggcctc
    13261 ggtgtcccct ccctggaccg cctggggggg ctggggggaa acagcctcct ccggggcggg
    13321 tgcggagccc ttcggctccc tccggatatc cttcttccat gcggtgatct cgccgatatc
    13381 ggtcttggtc tcgcctccca cgttcgcgag gtccagcttg tccagccggt ccaggatgga
    13441 ggcgtagtcc gcgtcctttg cctgcggttc ggcctccgga ggggccctcc tcttctcgac
    13501 ggtggtgtcg accttctcgg cctttatctc ccgccggacg gcctgctttc cgccgccctc
    13561 ggaggtgaga tcccgcttga cctcctcgat gccccggatg agggccacga agtcatcatc
    13621 cttccgggga gcctggggcc gggcctgggc cggttccggc cctgcccggg gggaaggctg
    13681 ggcaggtgcc ggggcccggg cctcggtctc gaggctcgcg agatccagct tctcgagctt
    13741 tgccatgacc gagtccgact cgggtccgag gccttcgacc tgcttcaccc cgtccttctc
    13801 gagggtctcc aggtgcttga ggaagtcctt cagttcatcc gcctgggccc tgccggggga
    13861 gagcgggggg gaggactgcg gctccgcggc cttccccttt ttcttctctg ccccctgccc
    13921 gccgggcccg ggttccctgt tctcgatgcc gaggagcccg aggcgcttca tcatcgcgtc
    13981 gagttccggt ttcttgtcag gttcctgtga tgtcatcttt accccccaat ctcgatgacc
    14041 gattccacgt accggtcatt gaagtcgacc atgaaggtgt agacctcctg ggcccggtcg
    14101 accgccatgc agtcgtaggt gccgttcagc agcttgaagc agaccttccc gtccgttgtc
    14161 gtgatgtcgc tcgcgatgat ctgccgtcct ttcttgatgg acacccggat tccctgctgg
    14221 ggaaccccct ccctcgcgag ggtgaggcag aggatccccg ggctccggtg ggtgcccccg
    14281 aaggacggca ggtcgtcgtc cagccgcttt ttcacatggc tcttgtcgaa gatccggcac
    14341 cgcgacgcga gggcatccac gatggacggg cttacgtcga agagctcgaa gggctcctgc
    14401 ccggtgagga ggatggccgc cttgtccccg aagagggtcc ccttgctgtc ggagaagatc
    14461 accccttcgg gggcgccggc ctggaaaacg atgtagaact tccggtcgtt ctcgtcatga
    14521 accccgatgc cgttcacggc atgggatccc gtgtacgcca tgagctcagg atagctgtag
    14581 ctcgcgacat aggttgaagt ggagagcact tcatcgatga tctggattat ttcccccgta
    14641 ccagcatcca ctcccggttc atggtgcctt caggtctgtg aaagactcta cgtaaggctc
    14701 cagtagtcgg tacaagtata tttcatcgtt aagatattat taaatgttct gtttttaaca
    14761 agcttgaatt aaaagataaa taaggaaaaa aactttgttt tatctttctg ggggaggatc
    14821 tctccccgat caccgggttg cgccgccgaa gagggtccgg gccagccggt ccacgaaccc
    14881 ttcctggacc tttaccggct cctccttgta ctccgagccg gcgaggtccg ccgcaatcct
    14941 ccggaaggcc cgggatgccc ccgaggtcgg gtacttgatg accaccgggg tcttgtaggc
    15001 cgccgccctc cggacgttcg cgtcctccgg gatcatgtcg atgacccgga cgcccaggac
    15061 gtcctcgacg ctgtgcctcc tgagctccgt gttctccatc ccggcccggt tcaggatcgc
    15121 cccgtagacc ttccccccga ccatctcggt aaggatcttg gtcttcaggg catcggccat
    15181 ggaggagagc tcggggttca cgacgaggat cacctcgtcc gcgatggcga gggccacgac
    15241 cccgtccttg ctgatcccgg caggggcgtc gatgaggagg aagtcgctcc ggccgatgag
    15301 atcccgcatg acatccttca tcttctcggg gtccgcgttc tggaagccct ggagggagat
    15361 gccggaggga accaccttca ggccgttcgg accctcatag atggcgtcgt ccaccgaggc
    15421 cttcccggca aggacctcgt ggagggtgac gggaacgctc tcgaggccaa ggacgagtcc
    15481 caggttggcc attccgatat cggcatccag gataaaggtc tccttcccga gctgggcgag
    15541 agaggtaccc aggttcaccg tgaccgtggt cttgccggtc ccacccttac ccgatgcaat
    15601 cgtgtacgct cgaaacatca gcacacccgt ttcatgacga gatccctgta aatctcatgg
    15661 ttaacttttc cctattcaaa tacttatccg ttgctgtcat gccaatcacc ctgtaccact
    15721 gttttctttc ccttcaccct catttccttg gcctgatttg gaacgtttcg acacaaggtg
    15781 ttccaggttc agctccgaga ggaggtcggc ggcgtcattc ttgaagctgt ccttcatgga
    15841 cttcaggtgc tcaaggccct tccggtccag ggatgccccc gtcggcccct tcgccgctcc
    15901 cggcctaccg gcgggggccg gcgccctctg gccgccggct ggctgccgtg cctcctccct
    15961 ccgaccggtc ttgaccgtga caaccttcac agatcttccc gtgcctcccc cgatactcac
    16021 gggccggatt accgtggggc tcggctggtt cttcgccccc ggtgcccctg ccggcctggg
    16081 ggggtcgacg aggaagggct cgttcgtccc cctcatctcg gtgagggtgt tccggggaag
    16141 gggggcgagg atgacatcga ccaaggggtt cccgataagc cggatactct ccagggcatc
    16201 ccgcccccgg atcccccggt actcggccag gatgtacctg ccctcctcca tgacgaggag
    16261 tgctcccgct ccggcgatgg gcatggcgca gtagccggtg aactgcctct tctccagctc
    16321 ggagaggagc gttcgcaggg ggatggcctt catgacctcc tcgcgggaag agggcggtat
    16381 ctgcatcagt ccttctccgc catgagcccc ttgagggcaa cccggatgag gccgaggttc
    16441 gcgtcgggat cggtgaagat gcagacgttc aggtcgccgt ggggggcgat gatgagcttc
    16501 cccttcccgg tctcgaggat gatctggttg aggggaccga tgtcgatctc ccgggcgatc
    16561 ttctttcccg cccggaagag atcctcggcg ttcgccgcca cctgctcgaa atcggcacgc
    16621 ccggccgact ggaccgcgaa gccctcgtag aaggtcgaga cggcgatgac gccgggctgc
    16681 ttcaggatcc gctggatcat cttctcgtcg atgagggggg gctccttgag gggcctctcg
    16741 ggctttttcg ggacccgggt gatctccacc ctcttcctct ggggtttcgg gatccggagt
    16801 tcaacctcct cggccggcgc cggagcgggg gaggggggct tcaccttctc ctcgggaatc
    16861 gccggcatct ccatgatggc gggtgccggc tcctccggct ccttctcggc ggggggcttc
    16921 accaggcccg accggcggag aagggaggcg atcctcgcct tccgttcagg ttccaccggg
    16981 cggatgacct tttcctcggg aggcttcggc accggttctg cctgctccgc aggggcggac
    17041 accggctccc tcaccccttc cttctccggg accggctccg gggcacgctt cagggggagg
    17101 gcgggggatt cggccgccaa aggtttcgcc ccgatcttgg ggagggcatc cttcacgggg
    17161 ggcaggggct ccctgggctc atcgaggacc tcgagccgct tgatgaaggc cttcagctca
    17221 tcgatatcgc tcctgccctt tgcctcgggt tcgggggtgg tctccccggc gggagccggg
    17281 gaaagagtga cggtttcctc cggttgcccg gagacggctc ccggggatcc cccgggcggc
    17341 ccgagctcct cctcaccctc ctggagttcc ctccggatgg aggtgatatc gagtcgcttg
    17401 atcctcttca tcatcttccc gaggtctacc ctgacccgct cctccggggc acggccttcg
    17461 gggatacccg caatcccggc ccccgggact gccggcccgg gctcttccac ggcggccggc
    17521 ccggcacctc ctgcagccgc tcccgccccg aaaacaccgg gttttggagg ggcctcccct
    17581 gctgcggaca ccggcggggc cggcgccttc tcgatcctgc ccgtttccct gaggcgcttg
    17641 atgatggacg agagctccgt ttccgcaacc ggagaagggg atggcgaagg cgacggggac
    17701 ggaagggtct ccctttcctc cggggcggtg atcttcttct cggccgcgac gggtggctgg
    17761 gatgggggaa gaggcacctc cttcacgatc cccggggcag gtttgacggg ttccgccggg
    17821 accggccgga caggttccgg cttcccgacc ttcggggccg cgaacctctt catcatggcc
    17881 gagaggacat tctcctcctc ccggggcttc gcctccctca cctgagccgg ctcacccgcg
    17941 gacggcacct tctccccctc gaggaggagc ccccggttct cgcagatgat catggcctcc
    18001 cggaactgct cctcgtcgta ccggatgatc tcgaactccg caagattctc cttctcgaga
    18061 atcgcgaggg aatcctttcc gcgggagagg acacccccgt gggccaggta ggccgcgacc
    18121 ggcctcccca tgttcacgag gatcccccct ttccccgagg aggtggtcac cttcaccgcc
    18181 ccgacgaagt tctggagctt gtgcgtcacg tccgagaggg aaccccggat cttccctgtc
    18241 ggaatcggtg ccgtggacat aggatatcta aagtgccgcg atgatccgtt ccttgttctt
    18301 cttcagctcg taccggatgc gcccgacatt ccccgtgttg tcagagacga tggcgatgag
    18361 ctcgtccttg gaaagggggg agaggaggat gggtcccttt tcgagctcca cgagcatctg
    18421 gttcagctcg cccttgccga gctcgttccc catcgcctcg gcggtgccca tgccggtgga
    18481 ggccatggca ccgagggcgt cggtgtcgac cttccccgag acggcgctct cgatgaccat
    18541 cccgtccctt ccgaccacca ccgcggcgaa taccccgtcc agcttcagga attcggccag
    18601 gatctgattc agcataagag attatcccct tttgatagcc ataatccttc tcctcaccgc
    18661 agagggacac cggaccggac gatctcgatg ggccaggacc cctttccgat ggcatgggtc
    18721 gcacaggaga tgcatggatc gtacgcccgg atgaccatct cgatccggtt cgcggcagcc
    18781 tcggggagcg tcccgtcctt cacgaacgtg cgggccaccg cctccacgct ccgatcgatg
    18841 gcgtagttgt tctggcaggt cgccacgatg aggttgcacc tctcgatgaa gccctggtcg
    18901 ttcaccgtgt agtcatggat gagggtcccc cggggggcct cgatgatccc gacgccccgc
    18961 ttcgccttca cccccgtgac cggcgaacgg atgttcgcac cggtgatctc gggatcctcc
    19021 aggagctcca cggccctctc gcagctcgcc aggaactcga cgtaccgggc caggttgtag
    19081 gcaaggggtg cctggaccgt cccgccgaac ttcgccctgt actccttcag ggcggcatcg
    19141 gcgagggggg ttcccatgga ggtgcagatg ttcagccggg cgagggggcc gacccggtag
    19201 aaggtcccgt ccgaaagcct cgtgaacttc aggtacgacc agtcctcgga gaactcgccg
    19261 atgtgcttcg cataatctgc accggtgaaa cccccgaggg tggccccgtc ctttccgatc
    19321 atctcgacgg atccctcata gggggaatac tttttgtccc tcgtcatggc gaggaacccg
    19381 gtctccaccg tcccgaggga gaggtcggtc ccgtccatca tgccccgggc cagctcccag
    19441 ccctccttcg caaaggcgag gccggccttt gccatgccga ggaggttccc gcgggcggtc
    19501 tcgtcgaggg cccgggacat tcccccgggg acggcgttgc tcgggtggat gggctttccc
    19561 cccaccgcct cggtgatccg ctgcccgagc ttcctgacct cgatggcctt cttcgcgagg
    19621 gcgggggact tcttcgcgat gccgatgacc gaccggtcgg ccgccggggc gtcgtggccc
    19681 acgaggaaat ccggggccgc gagcatgaag aagtggatgg catgggagtg gatgtactgg
    19741 gcatggttga ggagttcccg gagcttcttt cccgtcgcgg gaggctccgc cccgaggata
    19801 gcatccacgg ccttgaccga ggcgaggtgg tgtgaggtcg ggcagatccc gcagatccgg
    19861 ggcgtgatcc ggggagcctc ctccaccgcc gccccgatga ggaacttctc gaacccccgg
    19921 agttccatga cattgaagtg ggcccgctcg acctggttct ttgcgtcgag cgtgatcctc
    19981 acctgggcat gcccctcgag ccgggtgagc ggggagatgc ggatctcggt catacgttcc
    20041 gctccttgat gaggtcccgg atcttggaga tgcccttgtc ctccatgatg agggatccga
    20101 tggtgaaggg gtagaacgtg tgagcgacgt cgtaaatctc cttctcgatc tcggcctcgg
    20161 gcttgtcggt gaggtcggcg atccggcgga tcatcatgtt atagatgtcc cggcagggct
    20221 cccggaggat gtccagggaa ggcccgttgc acccgtggca ggggacgttg ctgtgcgggc
    20281 aggaggcacc gcaccggccg aaggtgaccg cacccaggca gaggtacccc tgcccgagga
    20341 ggcagtgctc ccggtccggg acccccgcgt accgccgctt cagctgccac ttctcgtggg
    20401 cccccatgat ccggtcgcac tccgagcaca ccgacttctt cgagagggag aggggctccc
    20461 cggatacgag ggccgggagg atcttcttca ggtacatctc cttcgggggg cagccggtga
    20521 tgtagtagtc cacctttgcg aggtccccga cggcaaaggc ccggtacagg aacgggggca
    20581 cgtccttcgg gatcaccccg tccggcttca ccgtctccac gccccggaag acgtggttga
    20641 acatgtcctc ccggttgaag agcatggaga gcccggagat gcctccgtag caggcgcagg
    20701 tcccgagggc aaccaccgtc ttcgcccgtt tccggatctt cttcagccgc tcctcgttct
    20761 cctcgttcct caccgccccg gtgacaaagg cgatgtcgat cccctccggg ggctccttca
    20821 cgtccatgat gacgggggcg tagacgatct cggccttccc gagcacgtca agaagggcct
    20881 cgtggaggtc gagtaccgcg atggtgcagc cggagcagga cgccagttcc tcgatggcaa
    20941 tcttcatggt cttcccccct actgcaggat gagcttcttc aggcaggcgt tcgggcccgt
    21001 atggacgatc tcgagcttcg cctttcttcc cgttatctcc tcgacagcgc ccacgacgta
    21061 cccgtagagg gtctggcaga ggggcccccc ctggacctgg ccgttccgcc ggagggtctg
    21121 ccggacgatg cagtcatgga acacgaggta gatgaaggtc gaaccgttct cctcgatgat
    21181 ataagagggc ttgtccgcgg gcttccagac ctcgatggtg aactggcccc ggaggatgtc
    21241 cgagagttcc tttaacgcct cctcgatatt ctccattttc cggaagtact tcgccacctc
    21301 gtggccgtac ttcctccccg cccggtacgt gacggcgttc gccccccttc ccgcgacctc
    21361 ctcgagggag cggatgatga ggccgtttaa cttcatgagg ccgtggaggg tatcctccag
    21421 ctcatcgggg tgagggatgc aggtgagggg cacgtcctcg gaccggtaca cgaggtccgt
    21481 agagaaggcc cccttcatct tctcgatggc accgtcggtc attaaatcac cctgttcggg
    21541 caatgttcca tcgccctcat tggctgatct gcactgcctt cggcagtcat cagatcaccc
    21601 gattcggggc aatgcgtcgc cccgacagtg taatttgaac ctccgagaga ggtcatcaaa
    21661 tcaccctgtt cagcatctcg cagacccgga tgtcgatgta gtgcctccgg gagggataga
    21721 tcccacggtg ctcgagggcc tgggccgggc agatctcgtg gcaggagagg cagcccatgc
    21781 agttctcggg atggaccggg accgagaggt cgtccctgag ctcgtagaca tgcatcgggc
    21841 agtccttcac acagaggccg cagccgttgc aggcgctgtc gtcgaccttg atggcgatca
    21901 tagaaacgtg ccccctgtag atgggagaaa ggtctacctg tgaagttaaa tacttatatt
    21961 gtccagtttc ccgcgcgaac gggaaccgtc aagcataaga atcaggccaa aaaaatatcc
    22021 agcatccatg atcgtccccc ttgtggtggc ctccgtggcg gtcctcgcca cccttgccta
    22081 tgcatccatc cttgacgtcc gggagcgttc ggtgcccgtc agggtgtggt atcccctcta
    22141 cctcgccgga tcggccgcgg tcatctggta cctcttcacc tccggatccg gctggggcac
    22201 cctttcgggc tacgccgccc ttattgtcac cctcgtctac ggcatcgagc tcgaggccga
    22261 ggacgggaag atccccgcaa aggtcctcct cctcgccctg ggggcgaccc tcctccaggg
    22321 gctcgcctgg ggtttcctgt ggagagagct cgggatcggg gccatcgccg gcttcgtcct
    22381 tctcgcggtc ttcctctggg agggcctcga gctcgagaga agggcgagcc tggacgagaa
    22441 gaccctttcg cctgcgtcgc gccagttcct cctctggcac cttcccgcca tcgtcctcct
    22501 ccaggccgcg ggatggggtg ccctcttcct ctcggggagg ggagggacgg gggacctcgt
    22561 cctcgtgctc gtcgccctct actccctcct cttcctcctc ttcgccgtga cgaacctctt
    22621 cggttttgca gacgccatcg cccttatcgc catcgccctc ttcgtgcccc ggttcccctt
    22681 tcagcccatc ctcgggtatc cccccctcga ttttctcccc ttctcggccc tcacgaacgc
    22741 ggtcatcctc aacctggtga ccccggtagg aatcgccgtc tggaacgcgg tgaaggggaa
    22801 ccgggccccc ttcccctaca ccttcttcgg gttcccggtg aggggggaca ccatcgagca
    22861 taccttcggc ttcgtgatgg aggagttcga g
//
LOCUS       DATGDI010000061         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_383842, whole genome shotgun sequence.
ACCESSION   DATGDI010000061 DATGDI010000000
VERSION     DATGDI010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383842"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1144)
                     /locus_tag="VKO45_02310"
     CDS             complement(158..1144)
                     /locus_tag="VKO45_02310"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HMA04740.1"
                     /translation="MLRVGMTYDLRTDYLEEGYSEEQTAEFDQPATIDAIEETLVSLG
                     YRTDRIGHVRALAKRLVAGDRWDLVFNIAEGLKGFGRESQVPCLLDAYDIPYVFSDPL
                     VLSLTLHKAMAKRVVRDLGIPTPDFAVVEDTGDIAAVSLPYPLFVKPLSEGTGKGINE
                     KSIVRDAGELDARCRSLLEAFSQPVLVETYLSGREFTVGIVGTGRKARAIAVMEVILR
                     ENAEKGVYSLVNKEYCEQFVDYALVTGEEAERVSTVALAAWRGLECRDGGRLDIRADA
                     SGVPHFLEVNPLAGLHPTHSDLPILSALAGVSYRDLLGMIMASAEERIRSGR"
     gene            complement(1233..>2329)
                     /locus_tag="VKO45_02315"
     CDS             complement(1233..>2329)
                     /locus_tag="VKO45_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HMA04741.1"
                     /translation="EWNDWRWQVRNRITGVEMLSRILWLSAEEEMAIHLRGEAIPLNI
                     TPYYASLLDPMDADQPMRRMVVPVMAEFERARDESDDPLGEDHDSPVPGLIHRYPDRV
                     LFLVTGFCSTHCRYCTRSRMVGRHEELHADRERWERAIAYIENTPTVRDILLSGGDPL
                     TLPDETLDWLLSRLRAIPHVELIRIGTKVPVVLPQRVTASLCHVLKRHHPLWMSIHFA
                     HPEELTFEVSQACNRLADAGIPLGSQTVLLAGVNDNVETMKRLVHDLVMNRVRPYYLY
                     QCDPITGSSHFRTPVSRGLEIIEGLRGHTTGYAVPTYVIDAPGGGGKIPLLPGYVVGH
                     DENHILLRNYQGRTFQYPDPAATAECEGRV"
BASE COUNT          439 a          712 c          740 g          438 t
ORIGIN      
        1 cgcctccaga tcgaggcaga acggcatggg aagcgcttcg tggccgagct ccttcagggc
       61 ggccgagacg gcctcgacct gtgtcaggac gtcccgctca tcggcggagg cgcaggaacg
      121 gacgtcactg tgaagtatga caaccctcat ggaattctca ccgcccggat cggatccttt
      181 cctcggcaga cgccatgatc atcccgagaa gatctcgata ggacacgcct gcgagggcgg
      241 acaggatggg aagatcggag tgggtcggat gaaggcccgc caggggattg acctcgagaa
      301 aatgcggcac cccggaggca tcggcccgga tgtcgagccg gcccccgtct ctgcactcga
      361 gccctcgcca tgcagccagg gcgaccgtcg acacccgctc ggcctcctca cccgtgacaa
      421 gagcataatc cacaaattgt tcgcagtatt ccttattgac gagggaatag acgccctttt
      481 ccgcattctc tcgcagaatg acttccatga ccgcaatggc gcgtgctttt cggcccgtgc
      541 ccacaatccc aacggtgaat tccctgccgg agaggtaggt ctcgacaaga accggttgcg
      601 aaaaggcctc gaggagagac cggcagcggg catcgagctc gccggcatcg cggacgatcg
      661 atttctcgtt gattccctta ccggttcctt ccgacaacgg cttgacgaag aggggatagg
      721 gaaggctgac ggcggcgatg tcccccgtgt cctcgacgac ggcgaaatcg ggcgtgggga
      781 tgcccaggtc gcgtaccacc cgcttggcca tggccttgtg gagggtgagc gagagcacca
      841 gaggatcgga gaagacgtag ggaatgtcgt aggcatcgag cagacagggg acctgggact
      901 cccgtccgaa tcctttcagg ccctcggcga tgttgaacac gagatcccac cggtctccgg
      961 caacaaggcg cttcgccaag gcacggacgt gcccgatccg gtccgtccgg tatcccaggg
     1021 agacgagcgt ctcctcgatg gcgtcgatcg tggcgggctg gtcgaactcg gcggtctgct
     1081 cctccgagta tccttcctcg agatagtctg tccgaaggtc gtaggtcatc ccgacccgta
     1141 gcatgggggt caccgtcttc ttttgaattt aagaaccccg ccgttttgtc ggcggggctc
     1201 ttctctggtt ctatccgatc atcgaggccg aatcaaaccc tgccttcgca ctcggcggtc
     1261 gcggccgggt ccgggtactg gaaggtgcgc ccctggtaat tcctgagcag aatgtggttc
     1321 tcatcatgac caacgacata cccgggcaga agcgggatct ttccgccgcc tcccggggca
     1381 tcgatgacgt aggtcggcac ggcgtacccc gtcgtgtgtc cccgcaggcc ctcgatgatc
     1441 tccagtcccc tggagacggg cgtccggaaa tgggacgagc cggtgatcgg gtcgcactga
     1501 tagaggtagt agggcctcac acgattcatg acgaggtcgt ggaccagccg tttcatcgtt
     1561 tcaacgttgt cgttgactcc ggccagcagc accgtctggc ttccaagcgg gatgccggcg
     1621 tcggccaggc ggttgcaggc ctggctcacc tcgaaggtca actcctctgg atgggcgaag
     1681 tggatgctca tccagagcgg atggtggcgc ttcaggacgt ggcagaggct cgcggtgacc
     1741 ctctgcggaa gcacgacggg caccttcgtc ccgatgcgga tcagttcgac gtgcgggatg
     1801 gcccggagcc gggacaacag ccagtccaga gtctcgtcgg gaagggtcag cgggtctccg
     1861 ccggaaagca ggatgtcgcg caccgtcggt gtgttctcga tgtaggcaat cgcacgttcc
     1921 cacctctctc ggtccgcatg gagttcttca tgcctgccga ccatccgcga gcgggtgcag
     1981 taacggcagt gggtggaaca gaaccccgtg acgagaaaga gaacacggtc gggatagcgg
     2041 tgaataagcc ccggcacagg gctatcatgg tcctctccga gtggatcgtc cgattcgtcc
     2101 cgggcgcgct cgaattccgc catcaccgga accaccatcc ttctcatggg ctgatcggca
     2161 tccatgggat ccagcaggct cgcatagtaa ggcgtgatat tcagcgggat ggcctccccc
     2221 cttaagtgga tggccatttc ctcttcggcc gagagccaga ggatgcgcga gagcatctcg
     2281 acgccggtga tccggttgcg gacctgccag cgccagtcgt tccattctg
//
LOCUS       DATGDI010000062         1546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_122281, whole genome shotgun sequence.
ACCESSION   DATGDI010000062 DATGDI010000000
VERSION     DATGDI010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122281"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..>1546
                     /locus_tag="VKO45_02320"
     CDS             45..>1546
                     /locus_tag="VKO45_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HMA04742.1"
                     /translation="MGPKITHKTERGLVRLHLKDEQEVLAAAGEIAKAAGNDLEGFLV
                     QPLVAGRREFVAGLVRDGQFGPVVMFGLGGVFTETLQDVSFRIAPFDEAEAGRMLDEL
                     RASALLGRFRGEEAANREQIIRALGGLSRLGVEHPEVAEVDINPLIVAPDGWVTAVDA
                     LVVLGEKTKPVPGHVRRDLSFLDAMLAPESIAIVGAKRASDDSWQNLMGIIKNFGYQG
                     RLYPINPGADEIEGLKVYPDLLSLPEKVDLVIISIPAPRVPAALEDCAASGNRNVHIF
                     TAGFKETGEEEGIRLQKEIEEIAERGGLRVIGPNCMGIYNPKLRLTTSIPAPAESGPV
                     AFVSQSGGLTGDLVVYAEQFGIYFSKAIRYGNALTLDSTDFLEYLAHDDETKIIALYL
                     EGVRDGGKLLHQITEINRVKPVIIMKGGLTESGARTVASHTGSLAGGEHIWSALFRQS
                     GAVKVTSLEEMTDVILAFLHLGAPQGRRVGVMGTGGGVGVSSADTFTQAGL"
BASE COUNT          289 a          451 c          495 g          311 t
ORIGIN      
        1 cggcgaaggc cgcctctatc gggtttcctg tagtccttaa gggattgggc ccgaagatca
       61 cccacaagac ggagcggggg ctcgtccggc tgcacctgaa ggatgagcaa gaggtccttg
      121 cggcagcggg ggagattgca aaagcggcag gcaacgatct cgagggtttc ctcgtgcagc
      181 ccctggtcgc aggtaggcgg gagttcgtgg caggtctcgt ccgtgacggc cagttcggcc
      241 cggtggtgat gtttggtctg ggcggtgtct ttaccgaaac cctgcaggat gtttcgttcc
      301 ggatcgcgcc gttcgatgag gcagaggccg gccgcatgct ggacgagctt agggcatcgg
      361 ctctcctggg caggtttcgc ggtgaagagg cggcaaaccg ggagcagatc atccgcgccc
      421 tcgggggctt gtcccgcctg ggcgtggagc atcccgaggt ggctgaggtg gacatcaatc
      481 ccctcatcgt cgcacccgac ggctgggtca ctgctgtgga cgccctcgtc gtcctcggtg
      541 agaaaacgaa acccgtccct ggccacgtga ggcgtgacct ctcctttctc gatgcgatgc
      601 tcgcacccga aagcatcgcc attgtcggtg ccaaacgcgc ctcggatgat agctggcaga
      661 acctgatggg aatcatcaag aacttcggtt atcaggggcg tctctatcct atcaatcccg
      721 gggccgatga aatagagggt ctcaaggtct accctgatct cctctccctg ccggagaaag
      781 tggatctcgt catcatttcc attccggcgc cgcgggtacc ggcggcactg gaggactgtg
      841 ccgcttcggg caacaggaat gttcacatct tcaccgccgg cttcaaggag acgggagaag
      901 aggaaggtat cagactccag aaggaaattg aggagatcgc ggaaaggggt ggtctccgtg
      961 tcatcggccc caactgcatg gggatttata atcccaagct ccggctcact acttcgatcc
     1021 cggcccctgc cgagagcggc cctgtcgcct tcgtcagtca gagcggtggc cttaccggtg
     1081 acttggtggt ctatgcggag cagttcggta tctatttcag caaggccatc agatacggga
     1141 atgccctcac cctggacagc acagatttcc tcgagtacct tgcccacgac gacgaaacga
     1201 agatcattgc cctgtatctc gagggagtcc gggacggggg caagctcctc caccagatca
     1261 cggagatcaa ccgggtaaag cccgtcatca tcatgaaggg gggcctgacg gagtctgggg
     1321 cacggactgt ggcatctcac acgggctccc tcgccggagg cgaacacatc tggtcggccc
     1381 tcttccgaca gagcggtgcg gtgaaggtga cttccctgga agagatgacg gacgtgatcc
     1441 tcgctttcct ccatctcggt gctccccagg gtcgccgggt gggcgttatg ggtaccggcg
     1501 gcggtgtcgg agtttcttcc gccgatacct ttacccaggc cggtct
//
LOCUS       DATGDI010000063         1723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_122288, whole genome shotgun sequence.
ACCESSION   DATGDI010000063 DATGDI010000000
VERSION     DATGDI010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122288"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VKO45_02325"
     CDS             <1..544
                     /locus_tag="VKO45_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF166 family protein"
                     /protein_id="HMA04743.1"
                     /translation="GSGNDLVREFAGRFGRPVLRATVDPESRRITGVTVVRDTPCGTA
                     SSAALRLEGLPCDPDEILKRCYEEHHNENAEHCCLAEMDPLYPVMQEAGDLLKDAAFA
                     ACGIPTTKEVILRILSDAGEMSIDDLKVRVVSPQARWEGREKGCTAGRTVDLYLEELA
                     GEGKVEAAGGRVKLRKGSPT"
     gene            complement(713..>1723)
                     /locus_tag="VKO45_02330"
     CDS             complement(713..>1723)
                     /locus_tag="VKO45_02330"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HMA04744.1"
                     /translation="AAVVGAVNNLLACGAVRPDHIYEMTVSGNSVMRDLFLGRAVDRL
                     GVFPFEPLSTSAVNTGASGLGIAIHPGGNVYALPLIGHFVGADTLALILATEMHEREE
                     VTMAIDIGTNTEIALGNRDRILVTSCASGPAFEGSGIACGTGSVEGAIQRVEIAAGGR
                     ISSETIGDGPPVGICGSGLIDSLAGLLEHGIIDRTGKFTGGRERFTLAEGDPAVYLDG
                     RDLDSLKLAKAAVAAGAKILLDRAGIGADSISRLFLAGAFGTYIDVAHAKRIGMLPDV
                     PLEKVMKVGNASVEGAAMVLLSRECREKAEELAGRITHVSLEQDPSFQDRFVEELAFP
                     EK"
BASE COUNT          315 a          552 c          558 g          298 t
ORIGIN      
        1 cgggagcggg aatgacctgg tccgggaatt cgcgggacgg ttcgggagac ccgtcctccg
       61 ggcaaccgtc gatccggaat cccgccgcat caccggggtg accgtcgtcc gcgacacccc
      121 ctgcggcacg gcctcctctg cggcgctcag gctcgaggga ctgccctgcg atcctgacga
      181 aatcctcaaa cggtgctacg aggagcacca caacgagaat gcagaacact gctgcctcgc
      241 ggagatggac cctctctacc ccgtcatgca ggaggcgggg gacctcctga aggacgcggc
      301 cttcgcggcc tgcggcatcc ccacgacaaa ggaggtcatc ctccggatcc tctccgatgc
      361 cggggagatg agcatcgatg acctgaaagt gagggttgta tctccccagg cccgctggga
      421 ggggcgagaa aaagggtgca ccgccgggcg tacggtggac ctctacctgg aggagctcgc
      481 tggagagggg aaggtggagg ctgcgggggg acgggtgaag ctgaggaagg gctcaccgac
      541 gtagtcgtat attctccttt ctcaactata agcagagtag cttttctgaa gaacccggtc
      601 gctctcgggg gcatacgagg ggatgaatat ccccgagtat ggagcaccca tcaggatgcg
      661 atgagccgaa ggcgagtcct gaggagcgtc catcgggggt catttcaaga ggtcattttt
      721 ccgggaacgc aagctcctcc acgaaccggt cctgaaagga cgggtcctgc tccaggctca
      781 cgtgagtgat ccttccggcc agctcctccg ccttctcgcg gcattcccgg gagaggagga
      841 ccatggccgc tccctcgacg gaggcgtttc ccaccttcat cactttttcc agtggcacgt
      901 cggggagcat cccgatgcgt tttgcatggg ccacatcgat atatgtcccg aaggcccccg
      961 caaggaagag ccgggagatg gagtctgccc cgatccccgc ccggtcaagg aggatcttcg
     1021 ctcctgccgc gacagcggcc tttgcgagct tcaggctgtc cagatccctc ccgtcgaggt
     1081 acaccgccgg gtccccttcg gcgagggtga aacgctcacg ccctccggtg aatttgccgg
     1141 tccggtcgat gatcccgtgc tccaggagcc cggcgaggga atcaatgagc cccgagccgc
     1201 agatacccac cgggggcccg tccccgatag tttcgctgga gatccttccc ccggcggcta
     1261 tctccacccg ctggatcgcc ccctccaccg agcccgtccc gcaggcgatc cccgagccct
     1321 cgaaggccgg gccggaggca caggaggtga cgaggatccg gtcccggttc ccgagggcga
     1381 tctcggtgtt cgtcccgatg tcgatcgcca tggtcacctc ttccctctcg tgcatttctg
     1441 tggcgaggat gagagcgagg gtatcggctc ccacgaaatg gccgatgagg ggaagggcat
     1501 agacattccc cccgggatgg atggcgatcc cgagtcccga cgcgccggtg ttcaccgcgg
     1561 aggtggacag aggttcgaag gggaagactc ccagcctgtc gaccgcccgg ccgaggaaga
     1621 gatcccgcat cacgctgttc cctgaaacgg tcatctcgta gatatggtcc ggtcgtaccg
     1681 caccgcaggc gaggaggttg ttgactgccc cgacgactgc ggc
//
LOCUS       DATGDI010000064         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_35108, whole genome shotgun sequence.
ACCESSION   DATGDI010000064 DATGDI010000000
VERSION     DATGDI010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35108"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VKO45_02335"
     CDS             complement(<1..390)
                     /locus_tag="VKO45_02335"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HMA04745.1"
                     /translation="MHHRIFITGGAGFIGSNFIRYIRRSHRDASILNFDKLTYAGNLK
                     NLEDVPAGGDYTFVKGDICDKDAVFQEFQRFAPDIVVNFAAESHVDRSIIDSSEFIRT
                     NIFGVKIMLDAAMKYGAEKFIQISTDEV"
     gene            complement(454..1182)
                     /locus_tag="VKO45_02340"
     CDS             complement(454..1182)
                     /locus_tag="VKO45_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA04746.1"
                     /translation="MPAVSVSHLSKVFRIPHEKRDTLFAALTGLVRPPNYENLYALRD
                     ISFTVDEGEMFGVIGENGSGKSTLLKVLARIIRPTSGSIELTKTVTPFLELGVGFQVD
                     FTAVENIRVYGMIMGLSKREIDGKIDEILKFGGLEKFRDTKLKNFSAGMYVRLAFSTA
                     IQTRPEILLLDEVLAVGDLDFQRKCLSVLEKFKREGVTIIFVSHDLNSVRQHCNRALL
                     LSKGEQVMMGETKEVVDRYEKGVQ"
     gene            complement(1182..1961)
                     /locus_tag="VKO45_02345"
     CDS             complement(1182..1961)
                     /locus_tag="VKO45_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA04747.1"
                     /translation="MTGDHLGALWKYRQLVWTIAWGDFKWRYKNSILGYFWSLLEPLI
                     MFIILYIVFSNLMRVQVEYYQLFLLLGIIMWNFFTKATNLGLNAIVGKPSLIQRVYFP
                     RDVLVIAGVITAMLQSLFESVVFILFMVYFAVPLSWNILYLLYILVIYFMLALGVALG
                     ISALNVYYRDIQFIWVVILQAGFFITPILYPLSIFSPQMQAIFRLNPIAQIIVTSRDV
                     ILYSVPPYWVSLAYAGVASLIVFIAGYVIFLKLEPRFAEAM"
     gene            complement(2041..>2739)
                     /locus_tag="VKO45_02350"
     CDS             complement(2041..>2739)
                     /locus_tag="VKO45_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04748.1"
                     /translation="NIDILFSPDLMGYLSGRHLKGGCFYRTNRIDVDADVPAGAPSRE
                     RLAYCGSHVIRINSRYFWSTTGNLGALLADISRNPGLVGYYLKNLVYKFRIPRLHYNA
                     CGDFTLMAKEDWDALRGYPELEMYSLHIDSLFLMAASASGLRERVLDSPCEIYHIEHS
                     IGSGVTPGMGQKILFERLDGEGIPYLSWRDCVRLFQNLKKQARGKDGTIHCNNDSWGL
                     PDASLPDHQVTACP"
BASE COUNT          604 a          756 c          799 g          580 t
ORIGIN      
        1 cacctcatcg gtcgagatct ggatgaactt ctctgccccg tacttcatcg ccgcgtcgag
       61 cattatcttc accccgaaga tgttggtcct gatgaattcg gaggagtcga tgatactccg
      121 gtccacgtga gactcggccg caaagttgac cacgatgtcc ggggcaaagc gctgaaattc
      181 ctgaaataca gcatccttat cgcaaatatc ccctttcacg aaggtgtaat cacctcctgc
      241 cggaacatcc tcgagattct tcaggtttcc ggcataggtg agtttatcga agttcaggat
      301 ggaagcatcc cggtggcttc tcctgatgta tcggatgaag ttgctcccga tgaatccggc
      361 tccacccgtg atgaaaatgc ggtgatgcat cgatggtact atgcaatttt ccggtgcatt
      421 aaaacttggg ggtgccatcc tttatgcagg tcttcattga acacccttct cgtaccggtc
      481 caccacttcc tttgtctctc ccatcatcac ctgctccccc ttgctgagga ggagtgcccg
      541 gttacaatgc tgcctcaccg aattgaggtc atgggaaacg aagatgatgg tcaccccctc
      601 cctcttgaat ttctcgagga ccgagaggca cttcctctgg aaatcgagat ccccgacggc
      661 gagcacctcg tcgaggagga gaatttccgg gcgggtctgg atggctgtgg agaaggcgag
      721 cctcacgtac atgcccgccg agaagttttt cagtttggtg tcccggaact tttccaggcc
      781 cccgaacttg aggatctcat cgatcttccc gtcgatctcc ctcttggaaa ggcccatgat
      841 catcccgtac accctgatgt tttcgacggc agtgaagtca acctggaacc cgacccccag
      901 ctcaaggaac ggggtcaccg tcttcgtgag ctcgatgctc cctgaggttg gccggatgat
      961 ccgtgcgagg accttgagga gggtgctctt cccgctcccg ttctcgccga tgaccccgaa
     1021 catctcccct tcatcgacgg taaaactgat gtcccggagg gcgtacaggt tttcgtaatt
     1081 gggaggcctc acgaggccgg tcagggccgc aaacagggtg tcgcgtttct catgaggtat
     1141 ccggaacacc ttggagaggt gcgagaccga gaccgcgggc atcacatcgc ctccgcgaac
     1201 cggggctcga gtttcaggaa tatgacgtac ccggcaatga agacgatcag cgaggcaact
     1261 ccggcatagg ccagggaaac ccagtagggt gggaccgagt acaggatcac gtcccgggag
     1321 gtgacaatga tctgggcgat cgggttcaac cggaagatcg cctgcatctg gggagaaaag
     1381 atcgagaggg ggtacaggat gggggtgatg aagaatcctg cctggaggat cacgacccag
     1441 atgaactgga tatcccggta atacacgttc agggccgaga taccaagggc cacgccgagg
     1501 gcgagcatga agtagatgac gaggatgtac aggaggtaaa ggatattcca ggagagggga
     1561 accgcgaagt agaccatgaa gaggatgaac accaccgact cgaagaggct ctgaagcatc
     1621 gccgtaatga cccccgcgat cacgaggacg tcccttggga aatacaccct ctggatgagg
     1681 ctcggttttc cgacgatggc attgaggccg aggttcgtcg ccttggtgaa gaaattccac
     1741 atgatgatgc ccaggaggag gaagagctgg tagtattcaa cctgtacccg catcaggttc
     1801 gagaagacga tgtagaggat gatgaacatg atgaggggtt caaggagcga ccagaagtac
     1861 ccgaggatgg aattcttata tcgccactta aagtctcccc atgctatcgt ccagacgagc
     1921 tggcggtatt tccagagtgc ccccaggtga tcgccagtca ttgccttcac tatacgttcc
     1981 cctgacagcc ttctttacct ttcgatccgg gtgcggcgga ggactcccgg ggtttcccgt
     2041 tcagggacaa gcggtcacct ggtggtcggg aagggatgca tccgggagac cccaggagtc
     2101 attattgcag tgaatcgttc catccttccc cctcgcctgt ttcttcaggt tctgaaagag
     2161 ccttacgcag tcccgccacg acaggtacgg gataccctcg ccgtcgagcc tctcgaagag
     2221 aatcttctgt cccatccccg gtgtgacgcc cgatccgatg ctgtgctcga tgtggtagat
     2281 ctcgcagggg ctgtcgagga cgcgctcgcg aagccccgag gcagaggcgg ccatgaggaa
     2341 aaggctgtcg atgtggaggg aatacatctc gagctcgggg tatccccgga gggcgtccca
     2401 gtcctccttc gccatgaggg tgaagtcgcc gcaggcattg tagtgcagcc gggggatcct
     2461 gaacttgtac acgaggtttt tcaggtagta cccgacgagg ccggggttcc tggaaatgtc
     2521 ggccagcagg gcccccaggt tcccggttgt cgaccagaag tacctcgagt ttatccggat
     2581 aacgtgggat ccgcagtaag cgagtctctc ccgtgagggc gcccccgccg gcacatcagc
     2641 atccacgtcg atccggttcg tccggtagaa acacccgcct ttcagatgtc tcccggagag
     2701 gtagcccatc agatcagggg aaaagaggat atcgatatt
//
LOCUS       DATGDI010000065         5390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_296831, whole genome shotgun sequence.
ACCESSION   DATGDI010000065 DATGDI010000000
VERSION     DATGDI010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296831"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VKO45_02355"
     CDS             complement(<1..279)
                     /locus_tag="VKO45_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04749.1"
                     /translation="MNMYCSPSGYPGDGPVHSIVVYNPLTRLPFMRIYTALFVSLLMV
                     SIFAAGCTSPGTLPQTQPVVTQNTSAMQVSGSSVPPEQLVAFVEKAYEF"
     gene            complement(311..384)
                     /locus_tag="VKO45_02360"
     tRNA            complement(311..384)
                     /locus_tag="VKO45_02360"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Val,seq:cac)
     gene            complement(388..458)
                     /locus_tag="VKO45_02365"
     tRNA            complement(388..458)
                     /locus_tag="VKO45_02365"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Gly,seq:tcc)
     gene            complement(489..1907)
                     /locus_tag="VKO45_02370"
     CDS             complement(489..1907)
                     /locus_tag="VKO45_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HMA04750.1"
                     /translation="MKMKSSLSHLRHLRGMIRDLRPRYEEDLGLLVGIPSVSSEPDHA
                     RDVRRCAEAAAGLLRRSGATAGIVPTDGHPVVHAALGTDPGNPTVAIYNHLDVQPADP
                     GEWNSDPFTLTISRGRYRGRGATDDKGPALAAMTAAAMAREAGIPLNFQFLWELEEEI
                     GSPNFAAFLRKRKERIPADSILVSDTIWADRGMPSIPYGLRGLLPVTLTLETGRADVH
                     SGVAGGAARNPLLEISGLLAECCEPETGRVRIRGFYDGIEDPGDERVKEFLAASGFSR
                     ERFARDLGLASLRHNLATDLDVVQAISARPTFEVHGISGGYAGPGSKAIVPHLAEAKV
                     SMRLVPDQDPARIARLFREFVKRQNPDVRVVFAPALEPYLGDHRGPYALAARSAMREA
                     FGREPVFSREGGSIGAVVAMDRILRVPISFLGLSLPSHGYHSVNEHFDWGQASGGILA
                     FLLYFEAIARIPRSRRRWVRKG"
     gene            complement(1957..2928)
                     /locus_tag="VKO45_02375"
     CDS             complement(1957..2928)
                     /locus_tag="VKO45_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HMA04751.1"
                     /translation="MPAALHRERPVIAVDLGATTTRVALIAPDGEILTRLAAPTPGAG
                     DSPKVITDHLAGLVRRILPAGRRSAPAAIGISAAGPIEHRRGAVVGPPNLPFREIPLE
                     GPLGDEFGLPVRLANDCHAGVLGEVSFGAGAGCRDLVYITLSTGIGGGVICNGRLVLG
                     REGNAAEIGHFHVDSTWNLPCGCGGSGHWEGYASGRFLPRFFARWSEASGLRVTDMPV
                     SAGEIFAAARRGEPGALRFLEALGTINGRGISDVIVAYDPARIILDGSVVLRNEDFIL
                     PYLLPRIDRFLPLPEIRLTALDGLAPLLGASVIARGYDTPLGSLLPR"
     gene            complement(2959..3840)
                     /gene="htpX"
                     /locus_tag="VKO45_02380"
     CDS             complement(2959..3840)
                     /gene="htpX"
                     /locus_tag="VKO45_02380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HMA04752.1"
                     /translation="MQWKRDFGLTMRVWFTMFLLLIVYLIFLTVLSALGVGVGGLIVV
                     AGGLAFVQYFFSDKLVLWTTRARIVQEQEYPELHAMVARLAAAVDLPTPKVAIVQTPV
                     PNAFATGRSPSHAVVAVTDSILRLLSPKELEAVIAHELSHVKNRDVLTLTIASFLAMV
                     AAIIMQNAWLFSMGSRRDQGNPWLIAWIVAIVVWLVSTLLIRALSRYREFAADRGSAI
                     ITRRPNDLITALEKISQRMDYVPPEQKQAVEGANMFYIIPALSGRSIMELFSTHPPLE
                     KRVAALERIEAELASGV"
     gene            4112..4762
                     /locus_tag="VKO45_02385"
     CDS             4112..4762
                     /locus_tag="VKO45_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phosphothreonine lyase
                     domain-containing protein"
                     /protein_id="HMA04753.1"
                     /translation="MGEAEEEDLSDVAYGLFEILLSRALREEGRPLFELVEAGSDFRP
                     AFTATFARFASDYPALAESLRVRFGSTDTLYALLTEGEGVVPTKTTLMYWIVQDPPDV
                     RSEDMDDEKAGKWLIFLQDDRVDELWRKIRNATASGELGISAKVSTSKPNPESRDERK
                     VIYVYTRDWADETDVMRVRERLREMGVIERIGYKRNIETFRGEYSEKGKKVTYYSA"
     gene            complement(4832..4978)
                     /locus_tag="VKO45_02390"
     CDS             complement(4832..4978)
                     /locus_tag="VKO45_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HMA04754.1"
                     /translation="MARFPEAEARLLNVKICMRCNARNALRATHCRKCNYTHLRPKNK
                     ERKD"
BASE COUNT         1051 a         1601 c         1835 g          903 t
ORIGIN      
        1 aaattcgtag gccttctcga cgaaggccac gagctgttcc ggcgggacag aggaaccgga
       61 aacctgcatt gcagaggtgt tctgtgtcac aaccggctgg gtttgcggga gagtacccgg
      121 ggaggtacaa cccgcagcaa agatcgaaac catcagcagg gaaacgaaga gggccgtgta
      181 tattctcatg aagggcaatc gggttaacgg gttataaacc acgatagaat gaaccgggcc
      241 atctcccggg tacccggagg gagaacagta catgttcatc gcctccgtcc ggctttcggg
      301 taaaagggaa tgggcccgct gagattcgaa ctcaggatct tcgccgtgtg aaagcgacgt
      361 catagccgac tagaccacga gcccccatgc atcgaccggg attcgaaccc gggctattgg
      421 cttggaaggc caatatcatg cctctagatc atcgatgcag catctcatga atattgcttc
      481 aagggtgatt agcctttcct gacccagcgt cggcgggaac gggggatccg ggcgatggcc
      541 tcgaagtaca ggaggaacgc gaggatccct cccgaggcct gcccccagtc gaagtgttcg
      601 ttcacggaat ggtacccgtg ggagggaagg gagagaccga ggaagctgat ggggacccgg
      661 aggatccggt ccatggcaac cacggcgccg atggatcccc cttcacggga aaagaccggc
      721 tctctcccga aggcctccct catcgccgag cgggcggcca gggcataggg cccccggtgg
      781 tcaccgagat agggctcgag ggcgggtgca aagaccaccc ggacatcggg attctgccgc
      841 ttcacgaact ccctgaagag ccgggcgatc cgtgccggat cctggtccgg gacgaggcgc
      901 atggagacct tcgcctcggc aaggtggggg acaatggcct tcgaacccgg gccggcgtag
      961 cctccggaaa tgccgtgaac ctcaaaggtg ggcctcgcgg agatggcctg taccacgtcc
     1021 aggtcggtgg cgaggttgtg gcggagggag gcaagcccca ggtcccgggc gaacctctcc
     1081 ctcgaaaaac ccgaggcagc gaggaactcc ttcacccgct cgtccccggg atcctctatc
     1141 ccgtcgtaga atccccggat cctgacccgc ccggtctccg gttcgcagca ctcggcgagg
     1201 agcccggaga tctcgaggag ggggttccgg gcggcccctc ccgcaacgcc ggagtggaca
     1261 tcggcccggc cggtctcaag ggtgagggtc acagggagga ggccccggag gccgtagggg
     1321 atggagggca tgccccggtc ggcccagatg gtgtcggaga cgaggatgga gtccgcggga
     1381 atcctctcct tccttttgcg aaggaaggcc gcgaagtttg ggctcccgat ctcctcctcg
     1441 agctcccaga ggaactggaa gttgagggga attcccgcct cccgggccat cgccgccgcg
     1501 gtcatggctg caagggccgg gcccttgtca tccgtcgcac cccggccccg gtagcgtccg
     1561 cgggagatgg tgagggtgaa gggatcggag ttccattctc cgggatcggc cggctggaca
     1621 tcgaggtgat tgtagatggc caccgtcggg ttcccggggt cggtgccgag ggcggcatgg
     1681 acgaccgggt ggccgtcggt cggtacgatc ccggcggtcg ccccggaccg ccggaggaga
     1741 ccggcggccg cttcggcgca gcggcggaca tcccgggcat gatccggttc ggagctaaca
     1801 ctcgggatgc caacgaggag acccaggtcc tcctcgtacc ggggcctgag gtcccggatc
     1861 attccccgga gatgccggag atgggaaagg gaggatttca ttttcatgga ccaccctgag
     1921 ctaatccccc cggacgataa gccttcccgg aggccttcac cggggcagca gcgacccaag
     1981 cggggtgtcg tagccccggg caatgaccga tgccccgagg aggggggcaa gcccgtccag
     2041 ggcggtgagc cgtatctccg gaagggggag gaaccggtcg atgcggggga ggaggtacgg
     2101 gaggatgaaa tcctcgttcc ggaggaccac cgagccgtcc aggatgatgc gggcgggatc
     2161 gtaggcaacg atgacgtcgg agatcccgcg gccgttgatg gtaccgaggg cctccaggaa
     2221 gcggagggca ccgggctctc ccctcctcgc cgcggcaaag atctccccgg cagacacggg
     2281 catatccgtt acccttaatc cgctggcctc ggaccaccgg gcaaaaaacc gggggaggaa
     2341 ccgccccgag gcatacccct cccaatggcc ggatcccccg cagccgcagg gaaggttcca
     2401 ggtgctgtcg acgtggaaat gcccgatctc ggcggcgttc ccctcacggc cgaggacgag
     2461 cctcccgttg cagatgaccc cgcccccgat acccgtcgag agggtgatgt agacgagatc
     2521 ccggcagcct gcgccggccc cgaaagatac ctcccccagg acccccgcgt ggcagtcgtt
     2581 cgcgagccgg acggggaggc cgaactcatc gccaagagga ccctcgaggg ggatctcccg
     2641 gaaagggagg ttcgggggcc cgaccaccgc cccccgccgg tgttcgatgg gcccggccgc
     2701 cgagatcccg atggccgccg gggccgaccg gcgaccggcg gggagaatcc tcctgacgag
     2761 tccggccagg tgatccgtga tgaccttcgg agaatccccg gctcccggcg tgggggcggc
     2821 gagccgggtg aggatctccc cgtcgggcgc gatcagggcg acgcgggtcg tggtggcacc
     2881 caggtcgacg gcgatgaccg gcctttcccg gtgcagagcg gcgggcatcg aggaaccccg
     2941 gggaggggaa aagggaggtt ataccccgga ggccagctct gcctcgattc tctcgagggc
     3001 ggcgaccctc ttctccaggg gcggatgggt ggagaagagc tccatgatgc tccggcccga
     3061 gagggcggga atgatgtaga acatgttcgc cccctcgacg gcctgcttct gctccggggg
     3121 gacgtagtcc atccgctggc tgatcttctc gagggccgtg atgaggtcat tgggcctcct
     3181 cgtgataatc gcgcttcccc tgtctgcggc aaactcccgg taccgggaga gggcgcggat
     3241 gagaagggtc gagacgagcc agacgacgat ggcgacgatc caggcgatga gccaggggtt
     3301 cccctggtcc cgccggctgc ccatggagaa aagccaggcg ttctgcatga tgatggcggc
     3361 gaccatggcg aggaaggagg cgatggtgag ggtgaggacg tcccggttct tcacatggga
     3421 gagctcgtgg gcgatgaccg cctcgagctc cttcggggag aggagccgga ggatggagtc
     3481 ggtcaccgcg acgacggcat ggctcgggct ccggccggtg gcgaatgcgt tcgggaccgg
     3541 ggtctggacg atggcgacct tcggggtggg gagatccacc gcggcggcca ggcgggcgac
     3601 catggcatgg agctcggggt actcctgctc ctgcacgatg cgcgccctcg tagtccagag
     3661 gacgagctta tccgagaaga agtactggac aaaagcaagc cccccggcca cgacgatgag
     3721 ccccccgacc cccaccccga gggccgagag caccgtgagg aagatgaggt acacgatgag
     3781 gaggaggaac atcgtgaacc agacccgcat ggtgagtccg aaatcgcgtt tccactgcat
     3841 ggtattccta tgattggaga atcctcttct taatacattc gaccatgggc aattcggggc
     3901 attttcgtgg cagcgggaga caaaccagca gatatctgtg gctttcagca cctccggatc
     3961 ttcggcgcgg atgggaaggg agtatcccgg cagtcatacc ctcccgaaca ccgtggatga
     4021 tccatcggac agggaggact tcaccagctc ctgagatgat tggcctcgcc atgggtaacc
     4081 ttttacagag gagcgtccga cactggattg tatgggcgag gcagaggaag aggacctttc
     4141 tgacgtggca tacggcctct tcgagatcct cctctcccgg gcactcaggg aggagggcag
     4201 gccccttttc gagctcgtcg aggccggctc cgacttcagg ccggccttca cggccacctt
     4261 cgccaggttt gcctcggatt acccggccct cgccgaatcc ctcagggtcc ggttcggatc
     4321 caccgatacc ctttacgcct tgctcaccga gggggagggg gtcgtcccca caaagaccac
     4381 cctgatgtac tggatcgtcc aggacccccc ggacgtccgg agtgaggaca tggacgatga
     4441 gaaggccggg aaatggctga tctttctcca ggacgaccgg gtggatgagc tctggcggaa
     4501 gatccggaac gcgacggcgt cgggggagct cgggatctcc gcgaaggtgt ccacctcgaa
     4561 gccgaacccc gagtccaggg atgagcggaa ggtgatctac gtctataccc gcgactgggc
     4621 cgacgagacc gatgtaatgc gggtcaggga gcgcctcagg gagatgggcg tcatcgagcg
     4681 gatcgggtac aagcggaaca tcgagacttt ccgcggcgag tacagcgaaa aagggaagaa
     4741 ggtcacctac tacagcgcgt gaacacccgg gatcagcttt ccgatcgatc ttccttgatg
     4801 aaggaaaaaa cgaacaaaaa atagaaatta ttcagtcctt ccgttccttg ttcttgggcc
     4861 gcaggtgggt gtagttacac ttgcgacagt gggtggcgcg gagggcgttc ctcgcattgc
     4921 agcgcatgca gatcttcaca ttcaggaggc gtgcctccgc ttcgggaaac cgtgccatga
     4981 ctcttgacct tcctcgttca gcctgtaatt aaaggaagcc ggcggtcata aggttttgtg
     5041 caagtcccta ccggaggtat aatttcgggt caatttcatc gtggggtgct ctcggggggc
     5101 tacgccaccc cgccgcacgg gcaccccccc gtgtccggaa ttcatgatga cgtctaacgg
     5161 aattagatgc ctatctccat ttggggaggc cgaagcggcg ggggtgagcg gagcgaaccc
     5221 ccaggagcgt ccggaaaatg gcaatgtagt tcggatcggg agatagtttg agatcttttg
     5281 ctatcctcag ggagcgactt agtagacagt ttcagaccgg cctgtatctc aggaataccc
     5341 tcccgttcct gaagggccgt tcctctagga gctgcaggtt catgcggttc
//
LOCUS       DATGDI010000066         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_180829, whole genome shotgun sequence.
ACCESSION   DATGDI010000066 DATGDI010000000
VERSION     DATGDI010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180829"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VKO45_02395"
     CDS             complement(<1..1074)
                     /locus_tag="VKO45_02395"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HMA04755.1"
                     /translation="MRLLMKFGGTSVGDGDRMKKVVDILEPYHEEGNELGVVVSAMSG
                     VTNQLIEMANEVVTCKEKPGIEPFITQLRDRHRKALEVAAPDYVEETGRILDGRLSDL
                     RNMFVGLNNLRELTPRSRDYLISFGERLSAPVLRAALRQRGIPSVVLDGCEAGLVTTA
                     THGDGIVLPESEGKIRSRVLPLLQDSVPVIMGYMGCTSQGITTTLGRSGSDYSATVVG
                     AAIGADEIWIWTDVDGIMTTDPRLVREARVLPTVSYLEAMELSYFGAKVMHPRSIEPA
                     MRKEIPIRVKNTFSPTQPGTCITKEKRKDRRVVKALTHITGVALINVNGAQMIGRPGV
                     ARAIFTALAEKEVNVMMISQGSSE"
     gene            1131..2759
                     /gene="tfrA"
                     /locus_tag="VKO45_02400"
     CDS             1131..2759
                     /gene="tfrA"
                     /locus_tag="VKO45_02400"
                     /EC_number="1.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043830 - thiol-driven fumarate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (CoM/CoB) subunit TfrA"
                     /protein_id="HMA04756.1"
                     /translation="MRAVDTVECHVLVLGSGGAGVRAAIEAEQYGDTVLVSKSLAGKG
                     GCTPMAEGGYNAALGEGDSCVLHKEDTIRGGAYLNDPVLADILVNEAPDRFRELVKWG
                     AVFDRTDAGGFAQRSFGGQRIPRTCYAGDRTGHEIMMTLLERLRAGNVTVHQEVSVID
                     LLKKGERVVGVVALDREGELMAFQADSTIIATGGAARIYDISTNSATGTGDGFAMGYR
                     AGAELIDMEMVQFHPTGAVYPYDARGRLVTEAVRGEGGILVNAKGERFMERYDPERME
                     LSTRDVVARSIAREVAEGRGTPHYGVYLDVTRLPPKQIEEKLPLMLEQFLAFGIDIRK
                     EKMEVSPTAHHFMGGMRITPKCETTVPGLYACGESAGGVHGANRLGGNSLAETQVFGK
                     RAGEAAGKAPSLGIRIDPRQLDPYRRRIEEFKNGMVNPVSVVKKLQEAMWKGAGISRN
                     VQDLERTLIDVDKLGKKRLLAQTPLNRAECFTVQNMCLVASIVIRSALLRGESRGAHL
                     RTDIFQSWDAANSPYGHTYISLHKAGIEERKVPA"
     gene            2779..>3323
                     /locus_tag="VKO45_02405"
     CDS             2779..>3323
                     /locus_tag="VKO45_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMA04757.1"
                     /translation="MKRFTVTVYRYNPAKDNRSRYQTYNVAVEEGARVLHVLHAIHAE
                     DPTLSYRYCCGTGQCGSCAVRVDGEPVLACMTEARDEMVIEPLNLPVKKDLLVDMEPV
                     LVKMASIAPKEEVCLPSREEVEALKPLRSCIECLACVSVCPAMEVAEFAGPLTMRQEM
                     RLAIDPRDSCDRISDAIRDGL"
BASE COUNT          567 a         1076 c         1135 g          545 t
ORIGIN      
        1 ctccgaggat ccctgggaga tcatcatgac gttcacttcc ttctctgcaa gggcggtgaa
       61 gatggcccgg gcgacgcccg gtctcccgat catctgggcc ccgttcacgt tgatgagggc
      121 gacccccgtg atgtgggtga gggccttcac cacccggcgg tccttccgct tctccttcgt
      181 gatgcaggtc ccgggctggg tggggctgaa ggtgttcttc acccggatgg ggatctcctt
      241 cctcatggcc ggttcgatgg accgggggtg catcaccttc gctccgaagt aggagagctc
      301 catggcctcc aggtacgaga ccgtcgggag gacccgggcc tcccggacga ggcggggatc
      361 ggtggtcatg atcccgtcga cgtcggtcca gatccagatc tcgtcggccc cgatggccgc
      421 cccgaccacc gtcgcggagt agtccgagcc gctcctcccg agagtcgtcg tgatcccctg
      481 tgaagtgcag cccatgtagc ccatgatgac cgggaccgag tcctggagga gggggaggac
      541 ccgggaccgg atctttccct cggattcggg gaggacgatc ccgtcgccgt gggtcgcggt
      601 ggtgacgagc cccgcctcgc agccgtccag gaccacggag gggatacccc gctgcctgag
      661 ggcggcccgg aggaccgggg cggagaggcg ttcgccgaag gaaatgaggt agtcccggga
      721 ccggggggtg agctcccgca ggttgttcag gcccacgaac atgttgcgga ggtccgagag
      781 cctcccgtcc aggatcctcc cggtttcctc gacatagtcg ggggctgcaa cctcgagggc
      841 cttccggtgc cggtcacgga gctgggtgat gaagggctcg atcccgggct tctccttaca
      901 ggtcacgacc tcgtttgcca tctcgatgag ctggttcgtc accccggaca tggcggagac
      961 caccaccccg agctcgttcc cctcctcgtg gtacggctcg aggatgtcca cgaccttttt
     1021 catgcggtcc ccgtctccca cggatgtgcc gccgaatttc atgagaagcc tcatagatcc
     1081 cctcgtaatc ctgacagttt ttatctatat gcgatccatg tactaataag atgcgtgcag
     1141 tcgatacggt ggagtgtcac gtcctcgttc tcgggagcgg cggcgccggc gtgagggcgg
     1201 ccatcgaggc cgagcagtac ggggacacgg tgctcgtctc gaagtccctc gcgggaaagg
     1261 gagggtgcac ccccatggcg gagggggggt acaacgccgc cctcggcgaa ggggactcct
     1321 gcgtcctcca caaggaggac accatccggg gcggggccta cctgaacgac ccggtcctcg
     1381 ccgacatcct ggtgaacgag gcgccggacc ggttccgcga gctcgtgaaa tggggggctg
     1441 tctttgaccg gaccgatgcc ggcgggttcg cccagcgatc cttcggcggg cagcggatcc
     1501 cgaggacctg ctacgccggg gaccggaccg ggcacgagat catgatgacc ctcctcgagc
     1561 gcctccgggc ggggaacgtc accgtccacc aggaggtctc ggtcattgac ctcctgaaaa
     1621 agggggagag ggtggtcggg gttgtcgccc tcgaccggga gggtgagctc atggccttcc
     1681 aggccgactc caccatcatc gccaccggcg gggcggcgag gatctacgac atctccacca
     1741 actccgcgac aggcacgggc gacgggtttg ccatgggcta ccgggcgggg gccgagctca
     1801 tcgacatgga gatggtccag ttccacccca cgggggcggt ctacccctac gacgcccggg
     1861 gccggctcgt caccgaggcg gtgcgggggg aggggggcat cctcgtaaat gcgaaggggg
     1921 agcggttcat ggaacggtac gaccccgagc ggatggagct ctcgacccgg gacgtggtcg
     1981 cccggtccat cgcccgggag gtcgccgagg gcaggggaac cccccactac ggcgtgtacc
     2041 tggacgtgac ccggctcccg ccgaagcaga tcgaggagaa gctgcccctc atgctggagc
     2101 agttcctcgc cttcgggatc gatatccgga aggaaaagat ggaggtctcg cccacggccc
     2161 accacttcat gggaggcatg cggatcaccc cgaagtgcga gaccacggtc cccggcctct
     2221 acgcctgcgg ggagtccgcg ggcggggtgc acggggcgaa ccggctcggg gggaattccc
     2281 tcgccgagac ccaggttttc ggcaagaggg ccggcgaggc ggccgggaag gccccgtccc
     2341 tggggatccg gatcgatccc cggcagctcg acccctaccg gcgccggatc gaggagttca
     2401 aaaacggcat ggtcaacccg gtctccgttg taaagaagct ccaggaggcc atgtggaagg
     2461 gggcggggat ctcgcggaac gtgcaggacc tggagcggac cctcatcgac gtggacaagc
     2521 tcgggaagaa gaggctcctc gcccagaccc cgctgaaccg ggccgagtgc ttcaccgtcc
     2581 agaacatgtg cctcgtggca tccattgtga tccggtcggc cctcctcagg ggcgagtccc
     2641 ggggggccca cctccggacg gacattttcc agagctggga cgcggcgaac tccccatacg
     2701 gccataccta catctccctc cacaaggcag ggatcgaaga aaggaaggtg cccgcgtgac
     2761 ccgaaaggag gtgcccgctt gaagcgcttc acggtaacgg tgtaccgtta caaccccgca
     2821 aaggacaacc gatcccggta ccagacgtac aatgtcgcgg tcgaggaggg agcacgggtc
     2881 ctccacgtcc tccatgccat ccacgccgag gatccgaccc tctcgtaccg gtactgctgt
     2941 ggcaccgggc agtgcggctc ctgtgccgtc agggtggacg gggaaccggt cctcgcctgc
     3001 atgaccgagg cccgggacga gatggtcatc gagccgctga acctcccggt gaagaaggac
     3061 ctcctcgtgg acatggagcc cgtcctcgtg aagatggcct ccattgcccc aaaggaggag
     3121 gtgtgcctcc cctcccggga ggaggtggag gcgttaaagc ccctgcgctc ctgcatcgag
     3181 tgcctggcct gcgtctcggt ctgcccggcc atggaggtcg ccgagttcgc cggccccctc
     3241 acgatgcggc aggagatgcg cctcgccatt gacccgaggg actcctgcga ccggatcagc
     3301 gatgccatcc gggacggcct ctt
//
LOCUS       DATGDI010000067         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_384429, whole genome shotgun sequence.
ACCESSION   DATGDI010000067 DATGDI010000000
VERSION     DATGDI010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384429"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VKO45_02410"
     CDS             complement(<1..366)
                     /locus_tag="VKO45_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MCM family protein"
                     /protein_id="HMA04758.1"
                     /translation="MAKGAAREEQVQAEVTDVAGEWRRFLVRRYNHQLAGLQREFPHN
                     RSLYIDYRTILAHGRLADAVLETPGKVIEDIREALVQHKTIDEKDRDRVNVRFTNLLV
                     KTPIRNIRADQMNRFLTVEG"
     gene            complement(370..1338)
                     /locus_tag="VKO45_02415"
     CDS             complement(370..1338)
                     /locus_tag="VKO45_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HMA04759.1"
                     /translation="MEENHTIWIEKYRPTKLAEMVGQREIVERLQSYVRSRSLPHLLF
                     TGSAGVGKTTAAVALAREFFGETWHMNFRELNASDERGIDVVRNQIKQFARTSPLEGA
                     SFKILFLDEADALTPDAQAALRRTMESYAATCRFILSCNYSSKIIDPIQSRCAIYRFR
                     PLDRGDIAEEIRRIAKREGLTVPEKAIDAIAYVAQGDMRKAINALQGAAILSPVIEEK
                     MVYEIAANARPEEIQEFLDLSLGGDFEGTERVLDRLLRERGIAPAELLGQCYRALVSR
                     KMDLALKVCLIDALGETDFRLSEGADSEIQMEALAARFVLAAKGGR"
     gene            1544..2344
                     /locus_tag="VKO45_02420"
     CDS             1544..2344
                     /locus_tag="VKO45_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04760.1"
                     /translation="MDRAGARMRERVEYLFAAELDRSDLVVPAAGGDGRPALITPTGL
                     VPRRLAITGALTEVEGKEGDYLHARVADPTGAFSLSSGWHRPEVTGTLAKIEPPAFIA
                     VTASPVLSSRSQARGVVLFPEAVAVADRVARDTWVIATAGATLGRLEGLCAALSGARQ
                     DPVLSQAIAHYRITPSFLGEIADIIDTALASLAPAPGGMPRAPGADPKSLVLTFLAER
                     PGEAIPVGEVLAELGRQGIGPGEGEQAVKELLDEGECYAPRKGTLRLA"
     gene            2349..>2880
                     /locus_tag="VKO45_02425"
     CDS             2349..>2880
                     /locus_tag="VKO45_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMA04761.1"
                     /translation="MALHFLPEGPALVIEGTERVLVIADLHIGIESDLDAHGVHVPSQ
                     TRGRLDRVLACIEAGRPDLVLLLGDVKHSVPLTTRQEYREIPTLLGEIRSRAPLRVAP
                     GNHDGGIERFLGAGELLPMRGALIDGVGYLHGHTYPDPSLLGHLLVIGHHHPMVSLRD
                     EVGCALRDRSYLLTSVD"
BASE COUNT          421 a          991 c          960 g          508 t
ORIGIN      
        1 cccctccacg gtgagaaacc tgttcatctg gtcggcccgg atattccgga tcggggtctt
       61 cacgagaagg ttcgtgaacc ggacattcac ccggtcccgg tccttctcgt cgatggtctt
      121 gtgctggacc agggcctccc tgatatcctc gatgaccttg cccggcgttt ccaggacggc
      181 atcggcaagc ctgccgtggg cgaggatggt ccggtagtcg atgtacaggg agcggttgtg
      241 ggggaactcc cgctggagcc cggcaagctg gtggttgtag cgccggacga ggaacctccg
      301 ccactcgccc gcgacatcgg tcacctcggc ctggacctgc tcctcccgcg cggcgccctt
      361 cgccatggct caccgcccgc ccttggcggc gaggacgaac cgcgcggcga gggcctccat
      421 ctggatctcg ctgtccgccc cttccgagag ccggaagtct gtctcgccca gggcgtcgat
      481 gaggcagacc ttcagggcga ggtccatctt ccgggagacg agggcccggt aacactggcc
      541 caggagctcg gccggggcga tccccctctc ccggaggagc cggtcgagga cccgctcggt
      601 cccctcgaag tcgcccccga gggagaggtc caggaactcc tggatctcct cgggacgggc
      661 gttcgcggcg atctcgtaga ccatcttctc ctcgatgacc ggggaaagga tggccgctcc
      721 ctggagggcg ttgatggcct tcctcatgtc tccctgggcg acgtacgcga tggcatcgat
      781 ggccttctca ggcacggtga gcccctcgcg cttcgcgatc cggcggatct cctcggcgat
      841 gtccccccgg tcgaggggcc ggaaccggta gatggcgcac cggctctgga tggggtcgat
      901 gatcttcgag gagtagttgc aggagaggat gaaccggcag gtcgcggcat agctctccat
      961 ggtccggcgc agggccgcct gggcgtcggg ggtgagggcg tccgcctcgt cgaggaagag
     1021 gatcttgaag gaggccccct cgagggggga ggtccgggcg aactgtttga tctggtttcg
     1081 gaccacgtcg attccccgct cgtccgaggc gttcagctcc cggaagttca tgtgccaggt
     1141 ctcgccgaaa aactcccgcg ccagggccac cgccgccgtg gtcttcccca cccccgcgct
     1201 cccggtgaag aggaggtggg gcagggagcg gctcctcaca taggactgga ggcgctcgac
     1261 aatctccctc tgccccacca tctcggcgag cttcgtgggg cggtacttct cgatccagat
     1321 ggtgtggttc tcttccatcg agcctcggtc ccccttcggg cgatcaagta taagcggcgt
     1381 gcgggagaga tctgtaagga tgtgtacctt tcgaaccggg atccccggat cccgcggttc
     1441 gccggatgga tctctccggc tacaggtatc agtgtgctgg ggaatattaa ttatcatttc
     1501 agttgcgggg tgaaggtgct ttgaggggaa gaccccggag gtcatggacc gggctggggc
     1561 gaggatgagg gagagggtag agtatctctt tgcggccgag ctggatcgct cggacctggt
     1621 ggtcccggct gctgggggag acggccggcc cgccctcatc acccctacag gcctcgtccc
     1681 ccggagactc gccatcaccg gggccctcac cgaggtggag gggaaggagg gggattacct
     1741 ccatgcccgg gtcgccgatc cgacaggggc cttctccctc tccagcgggt ggcaccgccc
     1801 cgaggttacc ggcaccctcg cgaagatcga gccccctgcc tttatcgcgg tgaccgcctc
     1861 gcccgtcctc tcttcccggt cacaagcccg gggggtggtc ctcttccccg aggctgttgc
     1921 cgtcgccgac cgggttgcgc gtgacacctg ggtcatcgcg accgccgggg cgaccctcgg
     1981 ccggctcgaa gggctgtgtg ccgccctctc cggcgccagg caggacccgg tcctctcaca
     2041 ggccattgcc cactaccgga tcactccttc cttcctcggg gagatcgcgg atatcatcga
     2101 caccgccctc gcctcccttg cccccgcacc gggagggatg ccccgcgccc cgggagcgga
     2161 cccgaagtcc ctcgtcctca ccttcctcgc ggaacggccg ggggaggcca tccccgtcgg
     2221 ggaggtcctc gcggagctcg ggcggcaggg gatcggtccc ggggagggag agcaggcggt
     2281 gaaggagctc ctcgacgagg gggagtgcta cgctccccgg aagggcaccc tcaggctcgc
     2341 gtgatcccat ggccctccat ttcctccccg aaggaccggc cctcgtcatc gaggggaccg
     2401 agagagtcct cgtcatcgcc gacctccaca tcgggatcga gtcggacctg gacgcccacg
     2461 gggtccatgt accgagccag acccggggga ggctcgaccg tgtcctcgcc tgcatcgagg
     2521 cgggaaggcc cgacctcgtc cttctcctcg gggatgtgaa acactcggtt cccctcacca
     2581 cgcgccagga gtaccgcgaa atccccactc tcctcgggga gatccgctcc cgcgcgcccc
     2641 tcagggtggc gcctggcaac catgacggcg ggatcgagcg cttccttggg gccggggagc
     2701 tcctgcccat gcggggagcc ctcattgacg gcgtggggta cctccatggc cacacctacc
     2761 cggacccctc cctcctcggc cacctcctcg ttatcgggca ccaccatccc atggtctccc
     2821 tcagggacga ggtgggctgc gccctccggg accggtcgta cctcctcacg tcggtggatg
//
LOCUS       DATGDI010000068         1955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_122809, whole genome shotgun sequence.
ACCESSION   DATGDI010000068 DATGDI010000000
VERSION     DATGDI010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122809"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VKO45_02430"
     CDS             <1..502
                     /locus_tag="VKO45_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HMA04762.1"
                     /translation="DRPLPMTPHHIQLADTRYHLGTSDPARIELIKIGWAEGALIQMP
                     IYEYRCADCRKKTTVVTLSVAAAVEPVCQHCGGRAMTKLVSRVMVKKSEESRLESLAD
                     PSALTGLDENDPKSVARWMKKMGREMGDEAGEGFDEEVDKAMEDAEREKEAGEDGGLP
                     GDDDGI"
     gene            533..1783
                     /locus_tag="VKO45_02435"
     CDS             533..1783
                     /locus_tag="VKO45_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HMA04763.1"
                     /translation="MTISTGIILFIGGGIFGAAVVLIVQLVQRKQSRELAKAIVLETE
                     SEKLEDLEAFLVRVKDSLGALSLEALHKTSEEFLRLAESRLGRMTESGEKDLESKKKL
                     IDHSLEAMRTDLQKMQDLVSGFEKDRVQKYGELSAHLKTAAEQTAKLQDTANHLRQAL
                     VSTKARGLWGERMAEDVLQLAGFVEGVNYLKQRALEGSGSRPDYTFNLPQGRKVNMDV
                     KFPLSSYLRYLETENDMEREVQKGQFLRDVRARIKEVTTRDYINPEDETLDYVLVFIP
                     NEQVYAFINQNDPAVIDEALRSKVILCSPFTLFAILAVIRQAMDNFTTAKAANDMLRL
                     IGGFNKEWQKFRESMDKMGRKIEDARDEFQRLTTTRCNKLERPLQKIEDLRRLKGIEP
                     GLALGEAEVQALDDAEEEEMQKRS"
BASE COUNT          423 a          592 c          656 g          284 t
ORIGIN      
        1 cgaccggccc ttgccgatga ccccgcacca catccagctg gccgataccc gctaccacct
       61 cgggacgagc gatccggccc ggatcgagct catcaagatc ggctgggcgg agggggccct
      121 gatccagatg ccgatctacg aataccgctg cgcggactgc cgcaagaaga cgacggtcgt
      181 gaccctctcg gtcgcggcgg cggtcgagcc ggtgtgccag cattgcggcg gccgggcgat
      241 gaccaagctc gtgtcgcggg tcatggtcaa gaagtccgaa gagagccgcc tggagagcct
      301 ggccgacccg tccgcgctca ccggcctcga cgagaacgac ccgaagagcg tggcccggtg
      361 gatgaagaag atgggccggg agatgggcga cgaggccggc gagggcttcg acgaggaggt
      421 cgacaaggcc atggaggacg cggaaaggga gaaggaggcc ggcgaggacg ggggcctgcc
      481 cggcgatgac gatgggatct aggagacggg gcgaggacca aggaggccgc ggatgacgat
      541 ctcgaccggc attatcctgt tcatcggcgg cggcatcttc ggggccgcgg tcgtccttat
      601 cgtccagctt gtccaaagaa aacagtcccg ggagctggcc aaagcgatcg tcctcgagac
      661 cgagagcgag aagctcgagg acctcgaggc tttcctggtc cgggtcaagg actcgctcgg
      721 ggccctttcg cttgaagccc tgcacaagac ctccgaggaa ttcctaaggc tcgcggaatc
      781 ccggctcggc cgaatgaccg aatccgggga aaaggacctc gaaagcaaga aaaagctgat
      841 cgaccactcc ctcgaggcca tgcggaccga cctccaaaaa atgcaggacc tggtctcagg
      901 gttcgagaag gaccgggtcc agaagtacgg cgagctcagc gcccacttga agaccgcggc
      961 cgagcagacg gccaagctcc aggacacggc gaaccacctc cgccaggccc tggtctcgac
     1021 aaaggcccgg ggcctttggg gagagcggat ggccgaggac gttctccagc tggccggatt
     1081 cgtcgagggg gtcaattacc tcaagcagag agcgcttgaa gggtcggggt cccggcccga
     1141 ttacaccttc aacctgcccc aagggcggaa ggtcaacatg gacgtcaagt tcccgctctc
     1201 gagctatctt cgctacctcg agacggagaa cgatatggag cgggaggtcc agaaggggca
     1261 gttcctccgg gacgtccggg cccggatcaa agaggtcacg acccgcgatt acatcaatcc
     1321 cgaggacgag accctggatt acgtcctggt cttcatcccc aacgagcagg tctacgcgtt
     1381 catcaaccag aacgatccgg ccgtgatcga cgaggccctc cggagcaagg tcatcctttg
     1441 ctcgcccttc acgctattcg ccatcctggc cgtcatccgc caggcgatgg acaacttcac
     1501 gacggccaag gcggccaacg atatgctccg gctgatcgga ggcttcaaca aggagtggca
     1561 gaagttccgg gaatccatgg acaagatggg ccggaagatc gaggacgcgc gggacgaatt
     1621 ccagcgactg accacaacgc ggtgcaacaa gctcgagcgg ccgcttcaga agatcgagga
     1681 cctgaggcgt ttgaagggga tcgagccggg gctggccctt ggggaggccg aggtccaggc
     1741 cctcgacgac gccgaggaag aggagatgca gaaaaggtcc tgaacggcgc gcctgacctc
     1801 gtcttagggt ctggggccga agatggctgt cccgatacgg acgagattgg ctccctcttc
     1861 gacagctacc cgccacgaat gggacatgcc catggagagg tgccgcatct cgacgcccgg
     1921 aatagaggcg tggcggaggg catcgaagac tctcc
//
LOCUS       DATGDI010000069         3508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_210051, whole genome shotgun sequence.
ACCESSION   DATGDI010000069 DATGDI010000000
VERSION     DATGDI010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210051"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1866
                     /locus_tag="VKO45_02440"
     CDS             <1..1866
                     /locus_tag="VKO45_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04764.1"
                     /translation="AGYDRMADVPLQRVFLVSAEPPAIRRLEQATDPRAKYSWAYLGK
                     SVIRARNIQKHLGSRFTRLPIGDLLQEAARSARKDYIEYTGSLSSSTGSPIWFLSSVS
                     ERNPFITTLLLYYSYIEAGRRLAGGSGGGDLVIIGESDAVLDTLAEVLAGSGNSRPRE
                     MIRLSAGGRTTHLGKILTKVIRKSWFIARFSFRILCARLLRARKGRFHSRSSQAGKHV
                     VLHSWTDARSFRDGEGFTNPYFGETGQGLERRYPSFLYLSDVLPTYWYPRAVLRLLKD
                     RRNVHTLEEFLSVQDLFSAVSTSVRHYPQPSDIPLFRDVDVSRIVMDEIEGDRSDTRL
                     EQAWVYSRAAGRLCREFDVGLFLYGFEHHTWEKMVCRAIREARPGAIVAGYAIAFINP
                     MYTCFSLSAYEQRGEPLPDIIFTSGEQGRSMLTRSGFREDMIRVGGAIRYPEFLTGKT
                     RRKSPRGNSIMLVLTGELNSSLELVYKALDAFSSLPGVRIVIKCHPTVPFSLLHRYLP
                     SLPDSFSVTDTPVEELLEDSDLVLYTESTVCVEAVAAGVPVFHVSSDHFLDINIFDGN
                     PAVPSSADPEEIRERSEEILEGKYILPSPDTLKELFMPVDTDTLVSSLLALLAGK"
     gene            complement(1896..3365)
                     /locus_tag="VKO45_02445"
     CDS             complement(1896..3365)
                     /locus_tag="VKO45_02445"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA04765.1"
                     /translation="MLLPTNISVLAASLAKAGISTRLFDTTLYRSEEKTFDEIKMDIL
                     QVKKFSYEGFVKTRPADQMYQDLRDLVEEYKPDLIAVTLVQDTFPLAKDLLSPLRDTG
                     IPVIAGGIFVTLNPMEVIGAEDIDMICVGEGEDALVELCRRMQENSDITRIKNIWVKK
                     PDGTILRNEMRDPVNLDSLPFIDFDVYADERKYRPMQGKIYMMLHVEADRGCPYDCTY
                     CSSPELRQLYAKMGCRYYRRKTVPRLIAELEYLKKKYNPGYVDINAESFLARPAGELE
                     EFSRLYGERIGLPFWCQSRPENISDDKIRFLKAAGCKNIQFGIEHGNEEFRRRMLRRT
                     YTNEQVVKGIEIVEKYGIDYTINNMLGFPEETRELFFDTVELNRQFRNPTTLNAYIFT
                     PYRGTEIYTYCVDRGYIDEKEKDPSHVRMIFGEIRMKNQPMTHDEILGLQRTFPLYAR
                     FPREMFPEIRKAERLDEEGNRAYEDLKALFYEKIYKMKY"
BASE COUNT          696 a         1011 c          980 g          821 t
ORIGIN      
        1 gccgggtatg atcgaatggc ggatgttcct cttcaaaggg tgttccttgt ctcagccgaa
       61 ccgccggcga taaggcgcct ggaacaggcc accgatcctc gcgcgaaata ctcctgggca
      121 tacctgggaa aaagtgtcat ccgggcaagg aatatccaga agcacctggg atcccggttc
      181 acgagactcc ccatcgggga cctgctccag gaggccgccc ggtcggcccg gaaggattac
      241 atcgagtata cggggtccct gtcctcctcg accgggagcc ccatctggtt tctctcctcg
      301 gtatctgaaa gaaacccctt catcaccacc cttctcctgt attattcgta tatcgaggca
      361 ggaagaagac tggccggagg gtccggcggc ggggatctcg tcattatcgg tgagagcgat
      421 gcggtcctcg acaccctcgc ggaggtactc gcgggatccg ggaattcccg tccccgggag
      481 atgatacgcc tgagcgcggg aggaagaacg actcacctcg gaaaaatact cacaaaggtg
      541 atcaggaaat cctggttcat agccaggttt tctttccgga tcctgtgtgc acgcctcctc
      601 cgtgcccgaa aggggcggtt ccattcccgg tcctcccagg ccgggaaaca tgtcgtcctg
      661 cacagctgga ccgacgcccg ctcctttcgg gatggggaag ggtttaccaa cccctacttc
      721 ggagagacgg ggcaggggct ggagagaaga tacccttcct tcctttacct ttcggacgtg
      781 cttcccacgt actggtatcc ccgggcggtt ctcaggctcc tgaaggaccg gagaaatgtg
      841 cacaccctgg aggagttcct ctcggtacag gatctttttt ccgccgtctc cacctcggtg
      901 aggcattacc cgcagccgtc ggatatcccc ctcttcaggg acgtggatgt ttcccgaatc
      961 gtgatggacg agatcgaagg ggatcgatca gatacccggc tggaacaggc atgggtatac
     1021 tcccgcgccg ccgggcgcct gtgcagggaa ttcgatgtag gtctctttct ctatggtttt
     1081 gaacatcaca cgtgggaaaa aatggtgtgc cgtgccatca gggaggcccg gcccggcgcc
     1141 atcgtagccg gttatgcgat cgccttcatt aatccgatgt atacctgttt ctctctctcg
     1201 gcatatgaac agagagggga acccctgccg gacattattt tcacgagcgg ggaacaggga
     1261 cgatccatgc tgacccggtc ggggttcagg gaggatatga tccgggtggg aggcgccatc
     1321 aggtacccgg agtttctcac cggtaagacc cggaggaaat ctccccgggg caactccatc
     1381 atgctcgtac tgaccggcga gctgaattca tccctggaac ttgtctataa ggcactggat
     1441 gccttttcct ccctccccgg cgtacgcatc gttatcaaat gccatcccac cgtgcccttt
     1501 tcccttctgc accggtatct cccctccctt cccgattcct tttcggtgac agatacgccc
     1561 gtcgaggaac tgctcgagga ttccgatctg gtcctctaca cggaatcaac ggtatgtgta
     1621 gaggccgtgg cggcaggtgt ccctgttttt catgtcagtt cggatcactt cctggacatc
     1681 aatatcttcg acggtaatcc ggcggtcccg tccagcgcag atccggagga gatcagagaa
     1741 cggtcagagg aaatccttga ggggaagtac atccttccct caccggatac cctgaaagag
     1801 ctcttcatgc ctgtcgatac cgataccctc gtctcctccc tcctggccct cctcgccggg
     1861 aaatgaaaaa cggaaaacag ggattttgcc agggcctaat acttcatttt atagattttt
     1921 tcatagaaca gggccttgag atcctcgtac gcccggttgc cctcctcatc gagccgctcg
     1981 gccttccgga tttccggaaa catctccctg gggaaccggg cgtacagggg gaaggtcctc
     2041 tggaggccaa ggatctcgtc atgggtcatg ggctggttct tcatccgtat ctccccgaag
     2101 atcatccgca cgtggctcgg atccttttct ttttcatcga tgtatcccct gtcgacgcag
     2161 tatgtgtaga tctccgttcc gcggtaaggg gtgaagatat aggcgttgag ggtcgtggga
     2221 ttcctgaact gccggttcag ttcaaccgta tcaaagaaga gttccctcgt ttcctcgggg
     2281 aatcccagca tgttgttgat ggtgtaatcg attccgtatt tctccacgat ctcgattccc
     2341 ttaaccacct gttcgttggt ataggtcctg cggagcatcc gcctcctgaa ctcctcgttg
     2401 ccatgctcga tgccgaactg gatgttcttg cacccggcgg ccttgaggaa tcgtatctta
     2461 tcgtcgctga tattctcggg ccgggactgg caccagaagg gaaggccaat cctctccccg
     2521 tacagcctgc tgaactcttc cagctcgcct gcgggacgcg caaggaagga ttcggcgttg
     2581 atatcgacgt acccggggtt gtacttcttt ttcagatatt cgagctcggc aatgagcctg
     2641 ggcaccgtct tccggcggta atacctgcat cccatcttcg cgtagagctg ccgcagctcc
     2701 ggagacgagc agtaggtgca gtcgtacggg cacccccggt cggcctcaac gtgaagcatc
     2761 atgtagatct tgccctgcat gggccggtat ttcctctcgt cggcgtagac atcgaaatcg
     2821 atgaagggga gggaatcaag attgacggga tcccgcatct cgttcctgag gatggttccg
     2881 tcgggcttct tgacccagat attcttgatc cgggtgatgt ccgagttttc ctgcatcctc
     2941 ctgcagagct cgacgagggc atcctcccct tctcccacgc agatcatgtc gatgtcctcg
     3001 gccccgatga cctccatcgg gttcagggtg acgaagatcc cgccggcgat caccgggatg
     3061 ccggtgtcgc gcagggggga gaggagatcc tttgcgaggg ggaaggtgtc ctgcacgagg
     3121 gtgaccgcga tgaggtcggg cttatattcc tccacgaggt ccctcagatc ctgatacatc
     3181 tggtcggcag gcctcgtctt cacaaacccc tcgtagctga atttcttcac ttggaggata
     3241 tccatcttga tctcatcgaa agtcttctcc tcggacctgt acagggtggt atcaaagaga
     3301 cgggtactga tgcccgcctt tgcgagggat gcggccagca cggatatatt cgtgggcagt
     3361 aacatggaca ggggcagatt gggatagata aataaaacct tgaacttttt ttccatggga
     3421 acctcccagt taacagtccg gtatcgcaac agcggggatg ggaaccatcg tcgcgtacag
     3481 atttatatga tctacaccgt ctggggat
//
LOCUS       DATGDI010000070        16693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_6485, whole genome shotgun sequence.
ACCESSION   DATGDI010000070 DATGDI010000000
VERSION     DATGDI010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 16693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16693
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6485"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1365
                     /locus_tag="VKO45_02450"
     CDS             253..1365
                     /locus_tag="VKO45_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMA04766.1"
                     /translation="MTGPEDEIRAIEEEISSTPYNKATSKHIGRLKAKLAKVKDEAVQ
                     RALKAGGGGEGFSVKKSGDATAVLVGFPSTGKSTLLNTLTGTHSEVASYAFTTVTVIP
                     GALEHRGAKIQILDIPGMIAGAAMGKGRGKEVISVVRSADMIVLLVDVWNEKHFDILI
                     RELADAGIRINRERPDITIKKTSIGGVHVGSVGKIGMDDEEIKAIMAEYRLVNAHVLI
                     RGGASQDDLIDTMLGNRVYVPAILVVNKVDLVDGATMRAMEGDLTGRFGESPLMISAV
                     SGYHMEELKDAIFDHLGFIRVYMKPQGEAADLEEPMILRRNATIEDVCRKLHREFVEK
                     FRYARIWGEAVKHPGQRVGLHYRVHDGDLVTVITRR"
     gene            complement(1373..2488)
                     /locus_tag="VKO45_02455"
     CDS             complement(1373..2488)
                     /locus_tag="VKO45_02455"
                     /EC_number="2.1.1.213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(10)-N(2))-dimethyltransferase"
                     /protein_id="HMA04767.1"
                     /translation="MDLVQVTEGGTEFLVPVQDPDCTFPPGSSPVFFNPRMALSRDAT
                     VLLLRGISPGSYLDAMGATGARGIRVARECGIPVTINDRDSLAVELIRRNAAHAGVSI
                     EVTQCDVNVLLSSRRFDAVDLDPFGTPSPFVDAAARSAKRYLFVTATDTAPLCGAHGP
                     AGARRYFAHPVNNEYHRETGLRTLLGFVVRETVKYDRGLEPLLCFTREHAVRLHLRVR
                     PGARAADDALGRIGFILQCDRCFHREEMAGLIPPCGKCPCGGSLVPVGPLWLGAINDP
                     GILSGMLDRLPTMPLAGGDALFRLLSLLRDELPTSSHYDYHRMAKALHCSPPPMDALL
                     RRIGDAGYRVSRAHYSGTAVKTDAPVEVVESAITGSR"
     gene            2548..2622
                     /locus_tag="VKO45_02460"
     tRNA            2548..2622
                     /locus_tag="VKO45_02460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2583..2585,aa:Arg,seq:cct)
     gene            2751..4736
                     /locus_tag="VKO45_02465"
     CDS             2751..4736
                     /locus_tag="VKO45_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HMA04768.1"
                     /translation="MNRQNGDPEPESLASRIHHALIGARRNIADPSLFHKIALIPILA
                     WIGLGADGLSSSSYGPPEAFGAIGNHLYLAIFLALATAVTVFVISYAYTRIIEHFPSG
                     GGGYIVATSTLGEQAGVVSGSALLVDYMLTITVSIAACAEAIFSFVPPQFQRIKLIFA
                     VLLILFLIILNLRGVKESVLVLAPIFLLFIVTHALLLGYGILSHSSGFGAVSSGIQAN
                     LALDLSTIGIIGILAIFFRAYSLGAGTYTGIEAVANGLQIMREPRVQTGKRTMAYMAG
                     SLALVAGGLFLCYLLWNVVPLPGKTLNAVLADLVFSGWAFGGVLAFLTILSEGALLMV
                     AAQTGFIDGPRVMANMAIDSWFPRRFSSLSERLTMQDGILLMGSAALVLLVYARGSVT
                     VLVIMYAINVFITFSLSEFGMSRFFFTRRHEDPLWSRHIAIHLTGLILCLTILSITIF
                     EKFTQGGWLTLLLTGSVIAICFLIKTHYQEVTEGIHRLDYHLNNVPAAGTVNTDPVDP
                     KKMTAVQLVTGFNGFGLHTFYSIIRSFPNLYENFIFVSVAVVDSGSFKGAGEVKALED
                     SVKEGLQKYVDLARTFGFAADYRTATGTDVVETAVALCQSIAQEFPNSTVYTGQIVFR
                     HESPFHKLLHNETAFAIQRNLQFSGITTVILPVQAEE"
     gene            4819..5685
                     /locus_tag="VKO45_02470"
     CDS             4819..5685
                     /locus_tag="VKO45_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HMA04769.1"
                     /translation="MTLAGLLVITRPVNSLVAGLAALLGYIVATGTLDPIALLLVPIV
                     FTITAGGNVFNDLRDLEIDRINRPLRPLPSGKVTPRAAGVLAAALFSAGLLLTLPAGL
                     PCVVIAVANSLLLILYARSLKGTALWGNVAVSYLAASIYPFGGALAGIAAMERTLPLA
                     GITFLAMLSRELLKDAEDVPGDTAAGARTVPIALGVKKTAVIAYACAVGAIAVSLLPV
                     VPWWGTGYLIGIGAADLVILFGAALALRCRTPDCVRHSRATGILKGGMYLALAIITGA
                     AILTGNLHIPVY"
     gene            5704..6123
                     /locus_tag="VKO45_02475"
     CDS             5704..6123
                     /locus_tag="VKO45_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HMA04770.1"
                     /translation="MKIYRWNETCIVPRGTYFKGNVRIEGDLLVPRDTHFWGRLVVEG
                     DLTLGPFSTVGAGVWCGNATIGDHVRIRGPLAAVGDVMVCDGAAIGMIRAARDVRLRP
                     GVRVGDVVSGRTILVQGKVESGRLLGRTVKVVGATFP"
     gene            complement(6397..7098)
                     /locus_tag="VKO45_02480"
     CDS             complement(6397..7098)
                     /locus_tag="VKO45_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HMA04771.1"
                     /translation="MLFDSPLWNSTMILLGEAAVVIAVFLIAMALFLLVIALLSVRNG
                     HLYLPRLMKTWFFLLEGLVKAICRFFGLDDRDLLSFSIRLQNSMNKKSFEAVPVEKRA
                     VFLPQCLRSANCPGKLTPEGIKCQGCGQCTTCEGIRDLGGMGYRVFIVPGSSFIKRMV
                     KKYRPEGIIGVGCILEVKEGLDLCDRLGIPGMGVVTLKDGCVETFVNWHEVLDVASLG
                     TPLATRPEGLRGPPE"
     gene            complement(7156..8172)
                     /gene="mtnA"
                     /locus_tag="VKO45_02485"
     CDS             complement(7156..8172)
                     /gene="mtnA"
                     /locus_tag="VKO45_02485"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HMA04772.1"
                     /translation="MPEPTIAWDEESGTIRFIDQTLLPERYQVVTCDTVERLIEAIRR
                     LEVRGAPALGVAGGMGVALAARRSRAATREAFLGEVEEAAAALTATRPTAVNLSWGVH
                     RVLARVREARDPGSAREAAAREATFLAEEDRATCQRIGDHGVNLIPDGATILTHCNAG
                     ALACTEWGTALGIVRSAARAGKRVRVLACETRPLFQGSRLTAWELSRDGIGVTVIPDG
                     SAASLMRRGRVDLVLVGADRITRDAVFNKIGTYMHAVCARHHGIPFYVAAPLSTFDPI
                     SEEKTVSIEERAREEVSRCGDRILVPEAARVENPAFDATPLSLVSAIVTEKGVFRPPY
                     DFAG"
     gene            complement(8180..9448)
                     /locus_tag="VKO45_02490"
     CDS             complement(8180..9448)
                     /locus_tag="VKO45_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HMA04773.1"
                     /translation="MAEVAVIGGGIAGIQAALDIANHGIRVHLIEREPSIGGHMAQLD
                     KTFPTNDCSLCILSPKMNEVSRHPLITLHTCTEVTSLRGKPGAYRLGLVTAPRFIDTE
                     KCNGCGECVLICPVEVYNRFDAGVGVRKAIYKPHAQAVPDLVIKDADHCIECGLCYDV
                     CDLDAIRKEEKEQRSEIRVASIVIATGYTVFDARKKGGLGYLTVPDVITSLELERMIN
                     ASGPTGGRVARLSTGKSPESVVFIQCVGSRDVSIGRPWCSCVCCMAAIKNAILLKEKN
                     PGMEVTICHNDIRTYGKGYEEYFTRAERMGIRFLKGLPGEVWSDARGPVLQVENTESR
                     EVEVMHPDLVVLSVGIGPGGGCDLAGILDIPLEESGFVKPLDDKLGPILTGKGGIYLA
                     GTATAPRDIPDSVAMGGAAAMRAFTDTLRE"
     gene            complement(9441..9929)
                     /locus_tag="VKO45_02495"
     CDS             complement(9441..9929)
                     /locus_tag="VKO45_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HMA04774.1"
                     /translation="MAGGGTGAAGFIAPSATVIGDVTLGEDTGIWFGAVIRGDRDRIE
                     IGAGSNIQDNAVVHTSRGFPARIGREVSVGHGAVLHGCTIRDRVLVGMGAIVMNGAEI
                     GEDSIIAAGAVVTEGTAIPPGSVVMGVPARVVKQASPEQKAGIRRNAESYVALAREYR
                     NG"
     gene            9960..10418
                     /locus_tag="VKO45_02500"
     CDS             9960..10418
                     /locus_tag="VKO45_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine adenylyltransferase"
                     /protein_id="HMA04775.1"
                     /translation="MKVMVGGTFDPLHAGHRKLLTRSFELAGSGGCVVIGLTTDEFAG
                     RKSHPVRSFAERRKALERFIQGKKFGAEFRIEPLADRYGPAPDEDFDALVVSEETLPV
                     GMEINRLRQEKGRRKVDIHQISCVLADDGRWISSTRIHRGEIDPEGRLVR"
     gene            complement(10411..10878)
                     /gene="pyrI"
                     /locus_tag="VKO45_02505"
     CDS             complement(10411..10878)
                     /gene="pyrI"
                     /locus_tag="VKO45_02505"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HMA04776.1"
                     /translation="MTPGQENRTLLVSQIKDGTVIDHITAGEALNVLTILGIAGTTSE
                     SLSIATNVASRKEGKKDILKIERRELRKEEVDRIALIAPHATINIIRDYEVVEKKGVN
                     IPRVIKGVVKCPNPGCISNANEPIESSFEVLGKGLHCCYCDWMITKDIASHII"
     gene            complement(10875..11771)
                     /gene="pyrB"
                     /locus_tag="VKO45_02510"
     CDS             complement(10875..11771)
                     /gene="pyrB"
                     /locus_tag="VKO45_02510"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HMA04777.1"
                     /translation="MRHIISIRDFERRDLDHLLRRAGEFAGGKKGGDLLAGKIVALLF
                     FEPSTRTRMSFASAMARLGGSCISVDSVEASSIAKGETLADTIRVVSGYTDAIVLRHP
                     KEGAARLASEFATVPVINAGDGAGQHPSQTLLDLYTIRQSMKLDGIEVGILGDLRYGR
                     TAHSLAYALSLYDVTLHAIAPKGLEFPEGLLHELRDRGMEVRSHHDVRKVIRDLDVLY
                     ITRIQKERFPDLGSYYDVASSYRITPETLEGVKDRLILLHPLPRVDEIAPAVDNTPYA
                     RYFEQSRNGVPVRMALLSEVMG"
     gene            12219..12503
                     /locus_tag="VKO45_02515"
     CDS             12219..12503
                     /locus_tag="VKO45_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YunC family protein"
                     /protein_id="HMA04778.1"
                     /translation="MQLPGKTARGFVYPLGPANLVAVVTDEGMVGCGAFDVAALEKFN
                     YPAARVRPSKGDSIATIDDLLEGIVKDANGPAQSRGVKVGMTGREALGKL"
     gene            complement(12624..13349)
                     /locus_tag="VKO45_02520"
     CDS             complement(12624..13349)
                     /locus_tag="VKO45_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMA04779.1"
                     /translation="MIEDGDRVLLVGKDREFYVQAGNRQLATDLGTVDLSVLVGAEPG
                     DLVKTHLGSEFVVRIPRPPDFFTHAARSGAPMLPKDIGLVIAHTGMNRRDEVLDAGTG
                     SGIAVIYYAGIAKSVKTYEIRSNFSKMAAKNVKAARLDNVEIVEGDVLSAEGKYDIVH
                     LDLQVSREHIVHAYDVLRPGGFLACYSPFLEQLFLVVEEAKKIFPEVHIRECIEREIV
                     RSLRGTRPSTRVSHSGYLTFARK"
     gene            complement(13346..13687)
                     /locus_tag="VKO45_02525"
     CDS             complement(13346..13687)
                     /locus_tag="VKO45_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HMA04780.1"
                     /translation="MFPGRVNPRKMKQMMDKMGMSMEPLEGVERVVISTAKGEYVFDA
                     AEVVAMTMQGVKTFQITGEPRFEAKGPVISEEDVRLVMEQTKAPEEKVRRTLAETRGD
                     IAEAILRLQAP"
     gene            complement(13669..14166)
                     /locus_tag="VKO45_02530"
     CDS             complement(13669..14166)
                     /locus_tag="VKO45_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HMA04781.1"
                     /translation="MSTEGSKARMLARVRVIADYQFGRGAGEGLFPDGCEFILSQTGR
                     VRQVLLGGLRLATLRAGDGRLTLSYTGACRLREAIPAPAYRVAIDPSVSDFIAKGKTA
                     FAKHVVSADPGIRAGDEVLLVTGADELLATGTALLSGAEMPSFNYGPAVKVRQGRESR
                     CFQEE"
     gene            complement(14111..15508)
                     /locus_tag="VKO45_02535"
     CDS             complement(14111..15508)
                     /locus_tag="VKO45_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HMA04782.1"
                     /translation="MDLLSDAAAAADLLAEAGEVTLVSHIDADGICSEAILSTALGRR
                     GIPVRPVFVRQLEPMTVGAIPKDRSFKLFTDLGSGQQNLLVEHGLTGDRILILDHHVG
                     QPVDGHSFREVNSLTYGHEKLAASGIAYLVARALDPANVDLARLAVIGNVGDMMARTD
                     LGLIGPSREIAEDGVRHGSVEVVKKDLNCFGISTRPVHLCLAYSDDPFIPGITGSPGG
                     ALQFLQRLGIALKHPSGRWLVWEEIPVEEKRRVISALAQQLLSHGRSPDRLLAEAYLF
                     PREKERPLRNASEFATVLNACGRWAKPTVGSHICKGDRGIHYREAERMLTHHRGMIRD
                     LLQFILEKGVTELSHLQHIHVGDRFPDTIIGIGAGMALSKLNRGKPILIMAEMMEDPL
                     VTKVSMRTNEEMVENGTDLQQAIVDATAEIGGSGGGHRIAAGAYIPKELEERFIHCVN
                     RRLEGQNARPGQGHR"
     gene            complement(15626..15871)
                     /locus_tag="VKO45_02540"
     CDS             complement(15626..15871)
                     /locus_tag="VKO45_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMA04783.1"
                     /translation="MKNRVRVYRAMYSLTQEELARHLGVTRQTIFAIEKEQYDPSLEL
                     AARIAQLFGATIEDIFIIPPMVTPLPTSEQDIRRSKS"
     gene            complement(15861..16256)
                     /locus_tag="VKO45_02545"
     CDS             complement(15861..16256)
                     /locus_tag="VKO45_02545"
                     /inference="COORDINATES: protein motif:HMM:NF021463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2178 domain-containing protein"
                     /protein_id="HMA04784.1"
                     /translation="MEVRGYWICSLLVIIAVAISLTWAVRIGNPYLAVIILVLAAGVL
                     YLCRKRIHEVIEDERDIRINERAAMKALEVFVPGGIIAGVVLYTISTPAADFTQAGLT
                     LGVAVTGLLLLYALFQTRFARIRGNTDEK"
     gene            complement(16272..16583)
                     /locus_tag="VKO45_02550"
     CDS             complement(16272..16583)
                     /locus_tag="VKO45_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04785.1"
                     /translation="MTEFVTSEKRGTVHLFERVKQQQVILTASVCVIALTIVLKNVLL
                     VPAEVLTRDMAVYTIIYLGFLVFAFRPEGNYEISGKVSPLTWDALVVLITLTVLAVYA
                     L"
BASE COUNT         2832 a         5459 c         5336 g         3066 t
ORIGIN      
        1 tcgggagtcc agggggtggc ccagacgggc tctcagctca taccgggcct ctccctcatc
       61 gtcgcggtga acctgggggc ggcggccatc gaggcactgg tcaccggctt cctcgttgag
      121 tacatcggca gggccaggcc gggatttctc gagggcgact gataccggga ggctgtccgt
      181 ccctccccgg ttcacccggc cttccgggaa acaggatatt ttctttacgt ttccccgcga
      241 atccatactg gaatgaccgg ccctgaggac gagatccgtg cgatcgagga ggagatcagc
      301 tccaccccct acaacaaggc tacctcgaag cacatcggcc ggctgaaggc caagctcgcg
      361 aaggtgaagg acgaggcggt ccagcgggcg ctcaaggctg gcggcggcgg ggaaggcttc
      421 tcggtgaaga agagcgggga tgcgacggcc gtgctcgtcg ggttcccctc gacggggaag
      481 tccacgctcc tcaacaccct caccgggacg cattcagagg tcgcctctta cgcttttacg
      541 acggtcaccg tgatccccgg ggccctcgag caccgggggg cgaagatcca gatcctggat
      601 atcccgggca tgatcgccgg cgccgcgatg ggcaagggca ggggcaagga ggtgatctcc
      661 gtcgtccgtt ccgcggacat gatcgtcctc ctcgtcgatg tctggaacga gaagcacttc
      721 gacatcctga tccgggagct cgccgacgcc gggatccgga tcaaccggga gaggccggac
      781 atcaccatca agaagacctc catcggcggg gtgcatgtcg gttccgtggg gaagatcggc
      841 atggacgacg aggagatcaa ggccatcatg gccgagtacc ggctcgtaaa cgcccatgtc
      901 ctcatccgcg gcggcgcatc gcaggatgat ctcatcgata ccatgctcgg caaccgggtc
      961 tacgtgcccg ccatcctcgt cgtgaacaag gtggatctgg tcgacggggc gaccatgcga
     1021 gccatggagg gggatctcac cggccggttc ggcgagtccc ctctcatgat ctcggcggtc
     1081 tcggggtacc acatggagga actgaaggat gcgatcttcg atcacctggg gttcatccgg
     1141 gtgtacatga agccccaggg cgaggcggcc gacctggagg agcccatgat cctccggcgg
     1201 aatgccacca tcgaggacgt ctgccggaaa ctccaccgtg agttcgtgga gaagttccgg
     1261 tacgcccgga tctggggcga ggccgtgaag caccccggtc aaagggtcgg cctccactac
     1321 agggtccacg acggggacct ggtgacggtc atcacgaggc ggtgagaact gcctacctac
     1381 ttcccgttat tgcgctctcc actacctcca ccggcgcgtc ggtcttcacc gccgtcccgg
     1441 agtagtgggc tctgctcacc cggtagcctg catccccgat cctccggagg agggcatcca
     1501 tcgggggcgg ggagcagtgc agagcctttg ccatccggtg gtagtcgtag tggctcgagg
     1561 tggggagctc gtccctgagg agggagagga gccggaagag cgcatccccc ccggcgaggg
     1621 gcatcgtggg gagccggtca agcatgcctg aaaggatccc cgggtcgttg atcgccccga
     1681 gccagagggg gccgacgggg acgaggcttc ccccgcaggg gcactttccg cacggcggaa
     1741 tgaggcctgc catttcctcc cggtggaagc accggtcgca ctggaggatg aacccgatgc
     1801 ggcccagggc atcgtcggcc gcccgggccc cgggccggac ccgcaggtgg agccgtacgg
     1861 cgtgctcccg ggtgaagcag aggaggggtt cgaggccccg gtcatacttc acggtctccc
     1921 ggacaacgaa accgagaagg gtgcggaggc cggtctcccg gtggtactcg ttgttcaccg
     1981 ggtgtgcgaa gtaccggcgt gcacctgccg gcccgtgggc gccgcagagg ggggcggtat
     2041 cggtcgccgt gacgaagagg tacctcttcg cggaacgtgc cgccgcatcg acgaaggggg
     2101 acggggtgcc gaagggatcg aggtctacgg cgtcgaacct gcgtgaggag aggaggacgt
     2161 tcacgtcgca ctgcgtgacc tcgatggaga ccccggcatg ggcggcgttc ctccggatca
     2221 gctccacggc gagggagtcc cggtcattga tggtgaccgg tatcccgcat tccctggcca
     2281 cgcggatccc ccgggcaccg gtcgccccca tggcgtccag gtagctcccg ggggaaatcc
     2341 cccggaggag gaggacggtc gcatcccggg aaagggccat cctcgggttg aagaacaccg
     2401 gcgaggagcc cggggggaag gtgcagtccg ggtcctggac ggggacgagg aattccgtcc
     2461 ctccttcagt cacctgaacc agatccatga acggcccccg gatagacgct accattatag
     2521 atatacctga gaaacgtaat aaaaaagggg cttgtggcct agccaggata aggcgtcact
     2581 ctcctaaggt gaatatcggg ggttcgaatc cccccaagcc cgtccgtttc ccctgatcaa
     2641 ccgcatgggt aactgaaagg atattatgcc gggatatcca ccttaatccc atggacggca
     2701 ggcggaggtt ccgccctgct cccatcccct gctttcccgg tggcggccct ttgaaccgac
     2761 agaacggcga cccggaaccg gagagtctcg cctcacggat ccaccacgcg ctcatcggag
     2821 cccggcggaa tatcgccgac ccctccctct tccacaagat cgccctcatc cccatcctcg
     2881 cctggatcgg cctcggggcc gacggcctct cctcctcgtc ctacggtccc cccgaggcct
     2941 tcggggccat cgggaaccac ctctacctcg ccattttcct tgccctcgcg acggccgtca
     3001 ccgtgtttgt catctcctat gcctacaccc ggatcatcga gcatttcccg agcgggggcg
     3061 gtgggtacat cgtcgccacc tcgaccctcg gggagcaggc cggcgtggtc tcgggatcgg
     3121 ccctcctcgt ggactacatg ctgaccatca ccgtctccat cgcggcctgt gccgaggcca
     3181 tcttcagctt cgtaccgccc cagttccaga ggatcaagct catcttcgcc gtcctcctca
     3241 tcctcttcct catcatcctc aacctgaggg gcgtgaagga gtcggtcctc gtcctcgcgc
     3301 ccatctttct cctcttcatc gtcacccacg ccctcctcct cgggtacggc atcctctcgc
     3361 acagctcggg cttcggggcg gtgtcctcgg ggatccaggc aaacctggcc ctcgatctct
     3421 cgaccatcgg catcatcggg atcctcgcca tcttcttccg ggcgtactcc ctcggcgcag
     3481 ggacgtatac gggcatcgag gccgtggcga acggtctcca gatcatgcgc gagccccggg
     3541 tccagaccgg gaagcggacc atggcctaca tggcgggctc cctcgccctc gtcgccgggg
     3601 gcctcttcct ctgctacctc ctctggaatg tcgttcctct ccccgggaaa accctgaatg
     3661 cggtccttgc cgatctcgtc ttctcgggct gggcctttgg gggtgtcctc gccttcctca
     3721 ccatcctctc ggagggggcc ctcctcatgg tcgcggccca gacgggattc atcgacgggc
     3781 cccgggtgat ggcgaacatg gccatcgact cctggttccc ccggagattt tcctccctct
     3841 ccgagcggct caccatgcag gacgggatcc tcctcatggg cagtgcggcc ctcgtcctcc
     3901 tcgtctatgc ccgcggttcg gtcaccgtcc tcgtgatcat gtacgccatc aatgtcttca
     3961 tcaccttctc cctttcggag ttcgggatgt cacgcttctt cttcacccgg aggcatgaag
     4021 atcccctctg gtcgcggcat atcgccatcc acctcaccgg cctcatcctc tgtctcacca
     4081 tcctttccat caccatcttc gagaagttca cccagggggg atggctcacc ctcctcctca
     4141 ccggatcggt catcgccatc tgcttcctca tcaagacgca ttaccaggag gtgaccgagg
     4201 ggatccaccg gctcgattac cacctgaaca acgtgcccgc cgcgggcacg gtgaacaccg
     4261 atccggtgga cccgaagaag atgaccgccg tccagctggt caccgggttc aacggcttcg
     4321 ggctgcacac cttctactcc atcatccgga gcttcccgaa cctctacgag aacttcattt
     4381 tcgtctccgt cgccgtggtc gactccggtt ccttcaaggg ggccggggag gtgaaggccc
     4441 tggaggactc ggtgaaggag gggctccaga agtacgtgga cctcgcccgg accttcggct
     4501 tcgcggccga ctaccggacc gcgacgggga cggacgtcgt ggagaccgcc gtcgccctct
     4561 gccagtccat cgcgcaggag ttcccgaact ccaccgttta cacggggcag atcgtcttcc
     4621 ggcacgagag ccccttccac aagctcctcc acaacgagac cgcctttgcc atccagcgca
     4681 acctccagtt cagcggcatt accacggtga ttctgccggt tcaggccgaa gagtgaggat
     4741 ccccggccgc ccggatggtc gttttctccc cggaatccca tgccttaata ccccaccggt
     4801 acaaccgttc cctcatccat gaccctcgcg ggcctcctcg tcatcacccg gccggtgaac
     4861 tccctcgtcg ccgggctcgc ggccctcctc gggtacatcg tcgccacggg aaccctcgac
     4921 cccatcgccc tcctcctcgt ccccatcgtc ttcaccatca cggccggagg gaacgtcttc
     4981 aacgacctcc gcgacctgga gatcgaccgg atcaaccgtc ccctccggcc cctcccgtca
     5041 gggaaggtga ccccccgggc ggccggcgtc ctcgcggccg ctctcttctc cgccggcctc
     5101 ctcctcaccc ttcccgcggg ccttccctgc gtggtcatcg cggtggcgaa ctccctcctc
     5161 ctcatcctct atgcccggtc cctgaagggg acggccctct gggggaatgt cgcggtctcc
     5221 tatctcgcgg cgagcatcta tcccttcggg ggtgcccttg ccgggatcgc cgcgatggag
     5281 aggaccctcc ccctcgccgg gattaccttc ctcgccatgc tctcccggga gctcctgaag
     5341 gacgccgagg acgtccccgg cgatacggcc gccggggccc ggacggtccc catcgccctc
     5401 ggggtaaaga agaccgcggt catcgcctac gcctgtgccg tcggggccat cgccgtctcc
     5461 ctcctccccg ttgtcccgtg gtggggaacg ggctacctca tcgggatcgg ggcggccgat
     5521 ctcgtgatcc tcttcggtgc cgccctcgcc ctccggtgca ggaccccgga ttgtgtccgg
     5581 cattcccggg cgacggggat cctgaagggg gggatgtacc tggccctcgc catcatcacg
     5641 ggggcagcca tcctgaccgg gaaccttcat attccggtat attgagagct ttttacggaa
     5701 gctgtgaaga tctaccggtg gaacgagacc tgcatcgtcc cgaggggaac ctacttcaag
     5761 ggaaatgtca ggatcgaggg tgaccttctc gtcccccgcg atacccattt ctggggcagg
     5821 ctcgtcgtgg agggggatct caccctcggg cccttctcca cggtcggggc gggtgtctgg
     5881 tgcgggaacg ccaccatcgg cgaccatgtc cggatccggg gcccgcttgc cgcggtgggg
     5941 gatgtcatgg tctgcgacgg tgcggccatc ggcatgatcc gcgcggcccg ggacgtgagg
     6001 ctccggccgg gggtgcgggt cggggacgtg gtgagcggga ggaccatcct tgtccagggg
     6061 aaggtggagt ccggccggct gctcgggaga acggtgaagg tcgtcggtgc gacgttcccg
     6121 tagggaacgg agctcgagca atgagaagcg ttgcgggtgc gtagctcccc atgaggggag
     6181 cgagcttgag cgatacaaag cacgcgagtt gcgacgttcc cgtaggggaa cggagctcga
     6241 gcaacgtgcg atgcatccaa tcagggaagc ggagcaggag cgacgcgagg cgtcgtgacc
     6301 tgaagcggtg cgaatagcga tgttcccgtt agggcacgga gctcgagcaa cgcgaaagcg
     6361 gtgcgaaggg ctgagtgaag gacttccccc gggaggctac tcgggaggac cgcgaaggcc
     6421 ctcggggcgg gtggcaaggg gggtccccag ggacgccacg tcgaggacct cgtgccagtt
     6481 cacgaaggtc tcgacgcagc cgtccttcag ggtcacgacc cccatccccg ggatcccgag
     6541 ccggtcgcag aggtcgagcc cctccttcac ctcgaggatg caccccaccc cgatgatccc
     6601 ctccggccgg tacttcttca ccatccgctt gatgaaactt gaaccgggca cgatgaagac
     6661 ccggtagccc atgcccccga ggtcccggat cccctcgcag gtggtgcact ggccgcagcc
     6721 ctggcacttt attccctcgg gcgtgagctt cccggggcag tttgccgagc ggaggcactg
     6781 ggggaggaag acggcccgct tctccaccgg gacggcctcg aaggacttct tgttcatgga
     6841 gttctggagc cggatcgaga aggagagaag gtcccggtcg tcgaggccga agaaccggca
     6901 gatggccttt acgagcccct ccaggaggaa gaaccaggtt ttcatgagcc gcgggaggta
     6961 aaggtgaccg ttcctcaccg agaggagggc gatgacgagg aggaagagcg ccatggcgat
     7021 gaggaacacc gcgatgacga ccgcggcctc gccgaggagg atcatggtgc tgttccagag
     7081 ggggctgtca aaaagcattt tttacttatt tgttggctgt ttcaggagat atagttcatc
     7141 ccgctcggct ttgcattagc ccgcgaaatc gtagggcggc cggaatactc ccttctcggt
     7201 cacgatggcg gataccaggg acaggggcgt cgcatcgaag gcaggatttt ccacccgggc
     7261 cgcctccggg acgaggatcc ggtccccgca ccggctgacc tcctccctgg cccgctcctc
     7321 gatggacacc gttttctcct ctgaaatggg atcgaaggtg gagaggggcg ccgcgacata
     7381 gaaggggatg ccgtggtgac gggcacagac ggcatgcatg taggtgccga tcttgttgaa
     7441 gaccgcgtcc cgggtgatcc ggtcggcacc cacgaggacg agatccaccc gccccctccg
     7501 catgagggag gcggcgctcc cgtcggggat caccgtgacc ccgatcccgt cgcgggagag
     7561 ctcccaggcc gtgagccgtg acccctggaa gaggggcctc gtctcgcagg caaggacccg
     7621 caccctcttt cccgcccggg cggcggaccg gacgatcccg agggcggtcc cccactctgt
     7681 gcaggcgagg gccccggcat tgcagtgggt gaggatggtt gccccatcgg ggatgaggtt
     7741 caccccgtgg tcgccgatcc tctggcaggt cgcccggtcc tcctccgcga ggaaggtcgc
     7801 ctcccgggcc gcggcctccc tcgccgagcc gggatcccgg gcctccctca cccgggcgag
     7861 gacccggtgt accccccagg agaggttcac cgccgtgggc cgggtggcgg tcagggcggc
     7921 ggcagcctcc tccacctccc cgaggaaggc ctcgcgggtg gcagccctgc tcctcctcgc
     7981 ggcgagggcc actcccattc ccccggcgac cccgagggcg ggggcccccc ggacctcgag
     8041 gcgccggatg gcctctatga gccgttccac ggtgtcgcag gtgacgacct ggtatcgctc
     8101 ggggaggagg gtctggtcga tgaaccggat ggttccggat tcctcgtccc aggctatggt
     8161 gggttccggc atggactcct cactccctga gggtatcggt gaaggcccgc atcgccgcgg
     8221 ccccgcccat ggcaaccgag tccggaatat cccggggtgc cgtcgccgtg ccggcgaggt
     8281 agatcccgcc cttccccgtg aggatgggcc cgagcttgtc atccaggggc ttcacgaacc
     8341 ccgactcttc gagcgggata tccaggatgc cggcgagatc gcagccgccg ccggggccga
     8401 tccccactga gaggaccacg agatcggggt gcatgacctc gacctcccgg ctctcggtgt
     8461 tctccacctg gagcaccggg ccccgggcat cggaccagac ctcgcccggg agtcccttca
     8521 ggaaccggat ccccattcgc tcggcacggg tgaagtactc ctcgtagccc ttcccgtagg
     8581 tccggatgtc gttgtggcag atggtcacct ccatccccgg gttcttctcc ttgaggagga
     8641 tggcgttctt gatggccgcc atgcagcaga cgcaggagca ccagggccgg ccgatggaga
     8701 catcccggga gcccacgcac tggatgaaga cgacgctttc cgggctcttc ccggtcgaga
     8761 gccgggcgac ccgcccgccg gtcgggccgc tcgcgttgat catccgctcg agctcgaggc
     8821 tcgtgatgac atccgggacg gtgaggtacc cgagcccccc cttcttccgg gcgtcgaaga
     8881 ccgtgtaccc ggtcgcgatg acgatgcttg ccaccctgat ctcggatctc tgttccttct
     8941 cctccttccg gatggcgtcc aggtcgcaga cgtcatagca gagaccgcac tcgatgcagt
     9001 ggtccgcgtc cttgatgacg agatccggga ccgcctgtgc atggggcttg tagatggcct
     9061 tgcgcacccc cacgcccgcg tcgaaccggt tgtagacctc cacggggcag atgaggacac
     9121 actcgccgca cccgttgcac ttctcggtgt cgatgaaccg gggggcggtc acgaggccga
     9181 gccggtaggc accgggtttc ccccggaggg aggtcacctc ggtgcaggtg tggagcgtga
     9241 tgaggggatg ccgggacacc tcgttcatct tcggcgagag gatgcacagg gagcagtcgt
     9301 tcgtcgggaa ggtcttgtcg agctgggcca tgtgcccccc gatggagggt tcccgctcga
     9361 tgagatgcac gcggatcccg tggttcgcga tgtcgagggc cgcctggatg ccggcgatgc
     9421 ccccgccgat gaccgccacc tcagccattg cggtactcac gcgccagggc gacgtagctc
     9481 tcggcgttcc tccggattcc tgccttctgt tctgggctgg cctgcttgac gaccctcgcc
     9541 gggactccca tgacgacgga ccccgggggg attgcggttc cctcggtgac caccgctccc
     9601 gcggcgatga tggagtcctc cccgatctct gccccgttca tcacgatggc ccccatgccg
     9661 acgaggaccc ggtcgcggat ggtgcacccg tggaggacgg ccccgtggcc caccgagacc
     9721 tctctcccga tccgggcggg gaagcccctg ctcgtgtgaa cgacggcgtt gtcctggatg
     9781 ttcgacccgg ccccgatctc gatccggtcc ctgtcgcccc ggatgacagc gccgaaccag
     9841 ataccggtat cctcgccgag agtgacatca ccgatgaccg ttgccgaggg cgcgatgaac
     9901 cccgcggccc cggttccacc accagccata ataccataaa ggtttcattt ctctcacgaa
     9961 tgaaggttat ggtgggcggg accttcgacc cgctccatgc cggccaccgg aagctcctca
    10021 cccgctcctt cgagctcgcg gggtccggcg gctgcgtggt catcgggctc accaccgatg
    10081 agtttgcggg aaggaaatcc cacccggtcc gttccttcgc agagcgcaga aaggcccttg
    10141 aacggttcat ccagggaaag aaattcgggg ctgaattccg gatcgagccc ctcgcggatc
    10201 ggtacggccc ggcacccgac gaggactttg acgccctcgt ggtctccgag gagaccctcc
    10261 ccgtggggat ggagatcaac cggctcaggc aggagaaggg gaggaggaag gtggacatcc
    10321 accagatctc ctgcgtgctc gccgacgacg gcagatggat ctcgagcacc cggatccacc
    10381 ggggggagat cgatcccgag ggccggctcg tcagatgatg tggctcgcga tgtccttcgt
    10441 gatcatccag tcgcagtaac agcagtggag ccccttcccg agaacctcga aggacgattc
    10501 gatgggctcg ttcgcattgg agatgcaccc cgggttgggg cacttcacca cgcccttgat
    10561 aacccggggg atgttcaccc ccttcttctc gacgacctcg tagtcccgga tgatgttgat
    10621 ggtcgcatgg ggggcgatga gggcgatccg gtccacctcc tccttcctga gttcccggcg
    10681 ctcgatcttg aggatgtcct tttttccctc cttccggctc gccacgtttg tcgcgatgga
    10741 gagggactcg gaggtcgtgc ccgcgatccc gaggatggtg aggacgttca gcgcctcccc
    10801 cgccgtgata tggtcgatga ccgtcccgtc cttgatctgg ctgacgagga gggtccggtt
    10861 ctcctgtccc ggggtcatcc catcacctcc gagaggaggg ccatccggac cgggacaccg
    10921 ttcctggact gctcgaagta ccgggcatag ggcgtattgt ccaccgcggg ggcgatctcg
    10981 tctacccgcg ggagcgggtg gaggaggatg agccggtcct tcaccccttc gagggtctcg
    11041 ggggtgatcc ggtagctgga ggccacatcg tagtaggagc cgaggtcagg gaaccgctcc
    11101 ttctggatcc gggtgatgta gaggacatcc aggtcccgga ttaccttcct cacgtcgtgg
    11161 tggctcctca cctccatccc ccggtcccgg agctcgtgga ggagcccctc ggggaactcg
    11221 agccccttcg gggcgatggc atggagggtg acgtcgtaga gggagagggc ataggcgaga
    11281 gagtgggcgg tccgcccgta ccggaggtcg cccaggatcc cgacctcgat gccgtcgagc
    11341 ttcatggact ggcggatggt gtagaggtca aggagcgtct gcgacgggtg ctgccccgcc
    11401 ccgtcgcccg cgttgatgac cggcaccgtg gcaaactcgg aggcgagccg tgccgcgccc
    11461 tccttggggt gccggagcac gatggcatcg gtgtacccgc tcaccacccg gatggtgtcg
    11521 gcgagggtct cccccttcgc gatggaggag gcctcgacgg aatcaacgga gatgcagctc
    11581 cccccgaggc gcgccatcgc ggaggcaaag gacatgcggg tccgggtcga gggctcgaag
    11641 aagaggaggg cgacgatctt ccccgcgagg aggtccccgc ccttcttccc ccccgcgaat
    11701 tccccggccc gcctgaggag atggtcgaga tcccttcgct caaaatccct gatggagatg
    11761 atatgccgca ccggatcacc ccgcccggtg cccggatctt tccaggaaca gcatcttgtt
    11821 cgaatccccc ttccggtctc ggtgtggact ttagagaatc atttcggcat caatcctgat
    11881 agaggtatcc acatggccgg agccgggacc ttgcggtaca aaaccggatc acggaaggga
    11941 tcccttcatg ggcgagggca ccccactccc atcagaggat ggaaatggac cgggtgatgg
    12001 gggcaaccat aggccttctc cttgtcgcag gtatcgccgt atccggcatc ctgctcacgt
    12061 ccggaaaggt gggggccgcc ggggcatcag gggaagaatc cttagaaggg gaatcagact
    12121 ccctcgggtc atccctgttt ggtgaacaac ccatgatcag aggattcttc tccccgccct
    12181 ccgagatggt tccgttgaca ggtatgcggg atgtaaaggt gcagcttccg ggaaagaccg
    12241 cgaggggatt cgtctacccc ctgggcccgg cgaacctggt ggcggtggtg accgatgaag
    12301 ggatggtggg gtgcggagcc ttcgatgttg ctgcccttga gaagttcaat tacccggcgg
    12361 cacgggttag gccctcgaag ggagactcca tcgcaaccat cgatgacctc ctcgagggga
    12421 tcgtgaagga tgcgaatggc cctgcgcagt cacggggtgt gaaggtcggg atgaccggaa
    12481 gggaggctct ggggaagctc taatatctat catttcctcg cgaaagtgag actcactttc
    12541 ttgcaaaagt gagactcccc ttcttgcgaa ggagaaactc actttcttgc gaaagtgaga
    12601 ttcacttcct tcggaagctc tccttacttt cttgcgaaag taaggtaccc cgagtgggag
    12661 acccgggtgg agggcctcgt gccccggagg cttctcacga tctcccgctc gatgcactcc
    12721 cggatgtgca cctcgggaaa gattttcttc gcctcctcaa cgacaaggaa gagctgctcg
    12781 aggaaggggg agtagcaggc gaggaagccc cccggccgga ggacatcgta ggcatgcacg
    12841 atgtgctcgc ggctcacctg gaggtccagg tggacgatgt cgtatttccc ttcggccgaa
    12901 agcacatccc cctccacgat ctcgacgttg tcaagccggg cggccttcac gttctttgcg
    12961 gccattttcg agaagttgga ccggatctcg taggtcttca ccgacttcgc gatcccggcg
    13021 taatagatga cggcgatacc ggatcccgtc cccgcgtcga gcacctcgtc cctccggttc
    13081 atgccggtgt gggcgatgac gaggccgatg tccttgggga gcatcggtgc cccgctccgg
    13141 gccgcgtggg tgaagaagtc ggggggccgg gggatccgga ccacgaactc tgacccgagg
    13201 tgggtcttta cgaggtcccc gggctcggcc ccgacgagaa ccgagaggtc cacggtcccg
    13261 aggtcggtgg cgagctgccg gtttcccgcc tggacgtaga actcccggtc cttcccgacc
    13321 aggaggaccc ggtccccgtc ctcgatcatg gcgcctggag ccggaggatg gcctcggcga
    13381 tgtcacccct cgtctcggcg agggtcctgc ggaccttttc ctcgggggcc ttcgtctgct
    13441 ccatgacgag ccggacgtcc tcctcggaga tcacgggccc ctttgcctcg aaccggggct
    13501 cgcccgtgat ctggaaggtc ttcaccccct gcatggtcat ggccaccacc tcggcggcgt
    13561 cgaagacata ctcccccttc gccgtggaga tgacgacccg ctccaccccc tcgaggggct
    13621 ccatggacat gcccatcttg tccatcatct gcttcatttt ccgggggttc actcttcctg
    13681 gaaacatcgg ctctccctgc cctgccgtac ctttaccgct ggaccgtaat taaatgaagg
    13741 catctccgcc ccggaaagga gggccgtccc cgtcgcaagg agctcgtcgg cccccgtcac
    13801 gaggagcacc tcgtcccctg cccggatccc gggatcggcg gagacgacgt gcttggcaaa
    13861 agcggtcttc cccttcgcga taaaatccga taccgaggga tcgatggcga cccggtatgc
    13921 cggtgcgggg atggcctccc tcagccggca cgcgccggtg taggagaggg tgagccgccc
    13981 gtcgccggcc cggagagtcg cgagccgcaa ccccccgagg aggacctgcc tcactcttcc
    14041 cgtctgggag aggatgaact cgcacccgtc ggggaaaagc ccctcccccg ctccccgccc
    14101 gaactggtag tcagcgatga ccctgacccg ggcgagcatt ctggccttcg agccttcggt
    14161 tgacacagtg gataaacctc tcctcgagtt ccttcgggat gtacgccccc gcagcgatgc
    14221 ggtgcccgcc gccggatccc ccgatctcgg ccgtcgcgtc cacgatggcc tgctggagat
    14281 cggtcccgtt ctctaccatc tcctcgttcg tccgcatgga gaccttcgtg accagggggt
    14341 cctccatcat ctcggccatg atcaggatgg gcttcccccg gttgagcttc gagagggcca
    14401 tcccagcccc gatcccgatg atggtatcgg ggaaccggtc cccgacatgg atgtgctgga
    14461 ggtgggagag ctcggtgacg cccttctcga ggatgaactg gagcaggtcc cggatcatcc
    14521 cccggtggtg ggtgagcatc cgctcggcct cccggtagtg gatgccccgg tctcccttgc
    14581 agatgtggct cccgacggtc ggtttcgccc agcggccgca ggcattgagc accgtggcga
    14641 actcggaggc gttccggagg ggcctctcct tctcccgggg aaagaggtac gcctctgcga
    14701 ggagccggtc cgggctccgg ccgtgggaga ggagctgctg ggcgagggcc gagatcaccc
    14761 tcctcttctc ctcgaccggg atctcctccc agacgagcca gcgtcccgag gggtgcttca
    14821 gggcgatccc cagccgctgg aggaactgga gggctccgcc cgggctcccg gtgataccgg
    14881 ggatgaaggg atcgtcgctg tacgcgaggc agaggtggac cggccgggtg gagatcccga
    14941 agcagttcag gtccttcttc accacctcca ccgagccgtg gcggaccccg tcctctgcga
    15001 tctcccggga ggggccgatg agcccgagat cggtccgggc catcatgtcc cccacgttcc
    15061 cgatgaccgc gagccgggcg agatccacgt tcgccgggtc gagggcacgg gcgaccaggt
    15121 aggcgatacc ggatgcggcg agcttctcat gtccgtaggt gagggagttt acctcacgga
    15181 acgagtgtcc atccaccggc tggccgacat ggtggtcgag gatgaggatc cggtccccgg
    15241 taagcccatg ttccacgagc aggttctgct ggccagaccc gaggtcggtg aagagcttga
    15301 aggatcggtc cttcgggatg gcccccacgg tcatgggctc cagctggcgg acgaagacgg
    15361 gccggacggg gatgccccgg cggccgaggg cggtggagag aatcgcctcg gagcagatgc
    15421 cgtcggcgtc gatgtgggac accagggtca cctcccccgc ttcggcgagg aggtcggccg
    15481 cggcggccgc atccgagaga agatccatta ctgtatggtt aggtcgatac aagtaataat
    15541 atccggcgtt cggggttgtt gccgggaaaa gggataccgt gctgatcatc cctgcaaccc
    15601 cccgatcccc gcgggggata ccctttcacg atttcgacct cctgatatcc tgctccgacg
    15661 tgggcagggg ggtcaccatg ggggggatga tgaatatatc ctcgatcgta gccccgaaga
    15721 gctgggcgat cctggcggcc agctcgaggg agggatcgta ctgctccttc tcgatggcaa
    15781 aaatggtctg ccgggtcacc ccgagatgtc gggcgagctc ctcctgggtg aggctgtaca
    15841 tcgcgcggta gacccggacc ctatttttca tcggtatttc cccggattcg tgcaaaacgg
    15901 gtctggaata aggcatagag gaggaggagc cccgtcaccg ctacccccag ggtgagtccg
    15961 gcctgggtaa aatcggccgc cggagtgctg atggtgtaga ggaccacccc cgcgatgatc
    16021 cctccgggca cgaacacctc cagtgccttc atcgcggctc tctcattgat gcggatatcc
    16081 cgttcatcct ctatgacctc atggatccgt ttcctgcaga ggtagagaac ccctgccgcc
    16141 aggaccagga tgatcaccgc gaggtatgga ttaccgatcc tcaccgccca ggtgagagat
    16201 attgccacgg cgatgataac gaggaggctg cagatccagt accccctcac ctccatacgc
    16261 cggcctcctc gtcacagggc atagaccgcg aggacggtga gggtgatcag gaccacgagg
    16321 gcatcccagg taagagggct gaccttcccc gatatctcgt aattaccctc gggcctgaag
    16381 gcgaacacga gaaatccgag atagatgatg gtatagaccg ccatgtccct ggtgaggact
    16441 tcagccggta caaggagcac attcttgagg acgatggtaa gagcgatcac gcagacgctc
    16501 gcggtaagga tcacctgctg ctgtttgacc ctttcgaaga ggtgtaccgt acccctcttc
    16561 tcggatgtaa caaattcggt catattctca ctgtaaataa ctctttacat tattatatta
    16621 aaactttgct tcatgttggt gttacttgtg tttttacaat ataaacttat aaaaagtggg
    16681 ctttctgcga atc
//
LOCUS       DATGDI010000071         9069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_122844, whole genome shotgun sequence.
ACCESSION   DATGDI010000071 DATGDI010000000
VERSION     DATGDI010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 9069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9069
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122844"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VKO45_02555"
     CDS             complement(<1..238)
                     /locus_tag="VKO45_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter"
                     /protein_id="HMA04786.1"
                     /translation="MIEELFTLEMHTYRDSPVHRLDARAKILAVLSVIIGIVAVPYTP
                     AVYAPALLLLLFFALLWGLSGISPLVYARRLALIL"
     gene            complement(235..1089)
                     /locus_tag="VKO45_02560"
     CDS             complement(235..1089)
                     /locus_tag="VKO45_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA04787.1"
                     /translation="MHLIETRNLTYRYPKNVLALQDVNFIAPRKARVAVIGANGAGKS
                     TLFKHFNGIFRPTSGSVLVHGEPITPENIREVRKYVGLVFQNPDDQIFAPTVEQDVAF
                     GPANLGLDEETIHHRVHEALRILGIEDLAHRPPHHLSGGEKKRVAIAGVIAMEPQALV
                     LDEPTAGLDPQGVRDLTGSLNALCRDYGMTVIFSTHDVSLVPELADSIYVMHRGQIVA
                     EGTVGEIFARAEMLRSFRLDVPPIPRLLRSLREHGVEISPAYSYAEAEQSLLSAFGKR
                     SSIEDRPP"
     gene            complement(1093..1476)
                     /locus_tag="VKO45_02565"
     CDS             complement(1093..1476)
                     /locus_tag="VKO45_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HMA04788.1"
                     /translation="MIPARTLLGVGLAVAILVGVAAVFLASGGPDGLESTTLVIRGEK
                     SLTGPAPSGAHVGENPGGFRFPSPFPDYSVGEGSGNIGSILAILLGIALVFGAAIGIP
                     RILSHRRPGRTKEEPGTPPSPPEDP"
     gene            complement(1473..2120)
                     /gene="cbiM"
                     /locus_tag="VKO45_02570"
     CDS             complement(1473..2120)
                     /gene="cbiM"
                     /locus_tag="VKO45_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HMA04789.1"
                     /translation="MHIPDGFIPLAQGLVYWAVALAFIALSLRWARRDMDEERIPLVA
                     VLAAGIFAIQSFNLPVGLGTTGHLVGGALAAIVLGSPYGAVFILTLVLLVQGFLFNDG
                     GITVMGANILNMGVIGGFVGFYSFRGIRAALGSLDVAAFSAAWLACLVPAFAAAVELY
                     LAGTFPLEGGLLAMGLYHALIGILEGAITVAAIRLIATARPDILGTEAGAGVAGP"
     gene            complement(2183..3331)
                     /locus_tag="VKO45_02575"
     CDS             complement(2183..3331)
                     /locus_tag="VKO45_02575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMA04790.1"
                     /translation="MYDVVVAGAGPCGSAAARACAEAGLRTLCIEEHGTIGRPVQCAG
                     LLSVAALSECKVSTRSILNTIWGARFIPSSGEPLEIDGGRPMAYVVDRGALDAEMARA
                     AAGAGAEFRLRTTVASVKGDRLITKGIRGREEIGFSILIAADGVRSSIARISGLGQAQ
                     SVLGGLQAEVRCGTGSRFVEIHPHASPEFFGWVIPTGDGMARVGLCGEGDLRPRLAAL
                     LRPFGGGILDLVSGALPLGVLPRTYGPRTLVAGDAAGMAKPTSGGGIYTGVRAARHAA
                     AVAVECCEKGSFRERDLARYEKLWKEDFGRELSVGMALYRARRNLSPADVDAVIGALR
                     DPEIVKEIEKTGDMDRPSSLFRRLALKPSVIRAAGIFLRGGLRSVLTD"
     gene            3568..4023
                     /locus_tag="VKO45_02580"
     CDS             3568..4023
                     /locus_tag="VKO45_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04791.1"
                     /translation="MQAGEILGGSIAVFIIVLIGLFVLPLAEVLTGQTMLIITIPIAI
                     AGALWFVLSLQRLVKGKGKNALRLRIQATRAGIIFIAAVFWSVLLVLTLRTSVLDAYD
                     SLPLDAKFLFWFGLNGLIAIRVYTSLLAHKPSEEEEPILAIDNIRIVQR"
     gene            4062..4430
                     /gene="pth2"
                     /locus_tag="VKO45_02585"
     CDS             4062..4430
                     /gene="pth2"
                     /locus_tag="VKO45_02585"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HMA04792.1"
                     /translation="MTSPGEREFRWKQCLVIRGDIRMSCGKISAQAAHAAVAAFERCD
                     RETRERWLAEGQKKVVLKVKDERSLLELKTIAEARGIAAALIQDAGLTEVPPGTTTAL
                     GLGPARSEEMDRITSDLPLL"
     gene            4427..5698
                     /gene="truD"
                     /locus_tag="VKO45_02590"
     CDS             4427..5698
                     /gene="truD"
                     /locus_tag="VKO45_02590"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HMA04793.1"
                     /translation="MRESPYPLEQRLGISWYATGTEGIGGYLRGEPEDFRVDELPTKS
                     WEESGEYLILRLTKRNWEQQRLVKDLARIFGISHRRISWAGTKDRRAVTSQYISIHGI
                     APEALERITLPGVTLEVAGRSDHPIALGELRGNRFLIRIRECEPTDLATRVEKVAGAG
                     ASGFPNYVGIQRFGVIRPVTHLVGERILRGDFEGAVLSYLGASFPSEPAEVREAREAY
                     LQGRDAKESLRHFPVRLTFERAMLHHLDGHPGDYPGALRALPPRLLSMFVSAFQSFLF
                     NTLLSRRCGGGIPLDGPAPGDRLIFTDGREDRVTAENLGAAVLQVRRKRAQIGLFVPG
                     SGVPEASIPDADPAGALLGELGIKPEDFAAASKFVRVAYSGVTRPVALLTDIDARLEE
                     RDVLLGFSLGPGQYATTVCREFMKADPMRMI"
     gene            complement(5667..6482)
                     /gene="sppA"
                     /locus_tag="VKO45_02595"
     CDS             complement(5667..6482)
                     /gene="sppA"
                     /locus_tag="VKO45_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HMA04794.1"
                     /translation="MGFLEEEVRRIEGRGWRRGLAVLGLIFLVLLAVFAAAWFLSYTK
                     ESSVAVIRVEGTLSTGDFSGPDATGSEFVGAEIRDAADNPLVEAIVLRVNSPGGTPAA
                     AQEITADMEYAKARKPVVVSMGDMATSAAYAVSAHADRIFADPDTITGGIGTVWIFTD
                     ISRWLGNEGYNVTVVKSGAWKDMASPYRNLTAGEEAYAQDLVNASFERFLADILEQRN
                     VSRAEIEDGRVVRGEEAVKIGLVDRLGNLHDAIGEARLLAGSGIRSSASGQPS"
     gene            complement(6484..7170)
                     /locus_tag="VKO45_02600"
     CDS             complement(6484..7170)
                     /locus_tag="VKO45_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HMA04795.1"
                     /translation="MPPRRERPHVLMMSEITADGKLTLKRGASSKILMKYMAPETEIL
                     LHETRADYDAIMVGANTIRIDNSFLTVRLVPGKSPLRVIPSGKAEIPLDANVLKKDAP
                     TVIAVAGEAPKDRVEAIRAKGVDVVVAGKDKVDLPLLMEILWRDYGVKKLMIEGGPTL
                     NWHMLRHRLVDEIRLIHLPFIVGGADTPSLVGGMHIDSESEMIRLALKRFYLCGSNLV
                     TEYDVLYGDG"
     gene            7264..7902
                     /locus_tag="VKO45_02605"
     CDS             7264..7902
                     /locus_tag="VKO45_02605"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HMA04796.1"
                     /translation="MNITALRPRLICHLEPVGRAFARAGFTPNQISLLSLLFGVAAMF
                     LFATRSFPAGSLLLLASGVLDLVDGHVSRIQNSGSRFGAVFDWIVDKYVDTLALLGVG
                     LSGIPILTRIIAAPAVADSGVVMVAILGSMMNTFIKPVTYAEAGFADRHHGKIDDPLE
                     GVGFFGRPETLLVLIAGGLSGLIWVSVLIIAVATNLSAMERIVYLRRHLSES"
     gene            complement(7924..8094)
                     /locus_tag="VKO45_02610"
     CDS             complement(7924..8094)
                     /locus_tag="VKO45_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04797.1"
                     /translation="MKRSVSALEVCVYLKRCEVQCSRFEDLSREIDLYQPDYYKYWAR
                     LKVQICSHLGSR"
     gene            complement(8179..9003)
                     /gene="artA"
                     /locus_tag="VKO45_02615"
     CDS             complement(8179..9003)
                     /gene="artA"
                     /locus_tag="VKO45_02615"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase A"
                     /protein_id="HMA04798.1"
                     /translation="MIEYLVLTSGIFFLLFLLPWRWRFHAAVLGWGSMVLYLIAEIPY
                     YLSLNNILYPALAILALPFLLVTGRRLLRKDPAILALSRGAAVAFLIYAPFAYLPALG
                     DFLIGVVTGQILSLLSAVGQPAALLAWNLIGRNGFRIEIILACTGIQSMAIMLGVAGA
                     VPTTRHQKILAFLLVVPTIYLLNLLRNIFVIAAYTGQWFPFLPEIASNGEYGYESFFW
                     AHNILAEGGALVALIAIAYALFVVIPDLGRWAGDLYTVYSGDLTGLYRRLTTRPGI"
BASE COUNT         1567 a         2939 c         3057 g         1506 t
ORIGIN      
        1 ggaggatgag ggcgagccgc ctcgcgtaca ccagggggga aatcccggag aggccccaga
       61 ggagggcgaa gaacaggaga aggagaaggg ctggggcgta gactgcgggg gtgtaaggga
      121 cggccacgat cccgatgatg accgagagga ccgcgaggat cttcgcccgg gcatcgagcc
      181 ggtggacagg actgtcccgg tacgtgtgca tctcgagggt gaagagctcc tcgatcatgg
      241 gggccggtcc tcgatggacg atcgtttccc gaaggcggag aggagggact gctcggcctc
      301 cgcataggag taggcggggg agatctccac cccatgctcc cggagggatc ggaggagcct
      361 cgggatgggg gggacgtcca gacggaagga ccggagcatc tcggcgcggg cgaagatctc
      421 cccgacggtt ccctctgcca cgatctgccc ccggtgcatc acgtagatgg aatccgcgag
      481 ttcggggacg agggagacgt cgtgggtgga gaagatgacg gtcatcccgt agtcccggca
      541 gagggcgttt aaggacccgg tcaggtcgcg caccccctgg gggtcgaggc ccgcggtggg
      601 ctcgtcgagg acaagggcct ggggctccat ggcgatgacc ccggcaatgg cgacccgttt
      661 tttctctccg cccgagaggt ggtggggggg ccggtgggcc aggtcctcga tcccgagaat
      721 ccgcagggcc tcgtgcaccc ggtggtggat ggtctcctcg tcgaggccca ggttcgccgg
      781 cccgaaggcc acgtcctgct caacggtagg ggcgaagatc tggtcatccg ggttctggaa
      841 gacgagaccc acgtacttgc gcacctcccg gatgttctcc ggcgtgatgg gctccccgtg
      901 gacgaggacc gaccccgagg tgggcctgaa gatcccgttg aagtgcttga aaagggtgct
      961 ctttccggcg ccgtttgccc cgatgaccgc cacccgggcc ttccgggggg cgatgaagtt
     1021 cacgtcctgg agggcgagga cgttcttcgg gtaccggtag gtaaggttcc gggtctcgat
     1081 gaggtgcatg ggtcagggat cctccggggg agagggaggt gtcccgggct cttccttcgt
     1141 ccttcccggg cgccggtggg acaggatccg cggaatgcct atggcggcac cgaagacgag
     1201 ggcgatcccg agcaggatgg cgaggatcga tccgatgttc ccgctcccct caccgacgga
     1261 gtaatccggg aagggggagg ggaaacggaa cccgccgggg ttttccccga catgagctcc
     1321 ggagggagcc ggcccggtga ggcttttctc tccccggatc acgagggtgg tgctctcgag
     1381 cccatccgga ccgccggagg cgaggaacac cgccgcgacc ccgacgagga ttgccacggc
     1441 aagtccgacg ccgaggaggg tgcgggcagg gatcacgggc ctgccacccc cgcgcccgcc
     1501 tccgtcccca ggatgtcggg gcgggccgtc gcgatgaggc ggatggccgc gacggtgatg
     1561 gccccttcaa ggatgccgat gagagcatgg tacaggccca tggcgaggag ccctccctcc
     1621 agggggaagg tgccggccag gtacaactcg acggccgcgg cgaaggcggg gaccaggcag
     1681 gcgagccagg ccgccgagaa ggcggcgaca tccaggctcc ccagggccgc ccggatcccg
     1741 cggaaggagt aaaagcccac gaagccgccg atgaccccca tattcaggat gttcgcgccc
     1801 atgacggtga tccccccgtc gttaaagagg aacccctgga cgaggaggac cagggtgagg
     1861 atgaagaccg ccccgtaggg ggaaccgagg acgatggccg cgagggcgcc ccccacgagg
     1921 tgaccggtcg tcccgaggcc gaccgggagg ttgaaggact ggatggcgaa gatgccggcc
     1981 gcaagcacgg caacgagggg gatccgctcc tcgtccatgt cccgcctcgc ccaccggagg
     2041 gagagggcga tgaaggcgag ggcgacggcc cagtagacga ggccctgggc gagcgggatg
     2101 aagccatcgg ggatatgcat ggcgcacctc accggtagta ttacataatt gtataaaagt
     2161 attacgaaat agttcgattc ttttaatccg taagaactga ccgaagcccg ccccggagaa
     2221 agatccccgc ggcacggatc acggagggtt tcaacgccag ccggcggaaa agggaggagg
     2281 gccggtccat gtcgccggtt ttctcgatct ccttcacaat ctccgggtcc cggagggccc
     2341 cgataactgc gtccacgtct gcgggagaga ggttcctccg ggcccggtac agggccatcc
     2401 cgacggaaag ctcccttccg aagtcctcct tccacagctt ctcataccgg gcaaggtcac
     2461 gctcgcggaa ggatcccttt tcgcagcact ccacggcaac ggcggcggca tgccgtgccg
     2521 cacggactcc ggtgtagatg ccgccccccg aggtgggctt tgccatgccg gcggcgtctc
     2581 cggcgacgag agtccggggg ccgtaggtgc ggggaagcac cccgaggggg agcgccccgg
     2641 acacgaggtc gaggatccct cccccgaagg gccggagaag cgccgccagc cggggccgga
     2701 gatccccctc accacacagg ccgaccctgg ccatgccgtc ccctgtcggg atcacccagc
     2761 cgaagaactc gggggaggca tgggggtgga tctccacaaa acgggaaccg gtcccgcacc
     2821 tgacctctgc ctgaagaccc cccaggacgg actgggcctg ccccagtccg gaaatccggg
     2881 cgatggagga ccggacaccg tctgcggcga tgaggatcga gaacccgatc tcctcgcggc
     2941 cgcggattcc ctttgtgatc agccggtctc ccttcaccga ggccacggta gtccggagcc
     3001 ggaactcggc gccggcaccg gcggctgccc gggccatctc ggcatcgaga gcgccccggt
     3061 ccacgacgta tgccatcggc cggcccccgt cgatctccag gggttccccg gaggacggga
     3121 tgaaccgggc accccaaata gtgttcagga tggagcgggt ggagaccttg cattcggaca
     3181 gggcggccac cgagaggagg cccgcgcact gcacggggcg gccgatggtg ccgtgctcct
     3241 cgatgcagag ggtccggagg ccggcctcgg cgcatgccct cgcggcggca gaaccgcagg
     3301 gccctgcccc ggccacgaca acgtcataca tcccatctcc cgttgatcac gctggttctt
     3361 atccctgccc gcccttagaa ccgcgccaca ccgtccttag atccgcgcca tgcccatcct
     3421 tggttcctcg ctccgcccgc ccagggttcg cgctatgccc tgaccccgac atccgtctcc
     3481 gccctggcct catgtccgag cccccgggcc ccatacaggt tcccgtcctg cgaaatctat
     3541 atgcaagacc ctatgacatc atcaggtatg caagctgggg agattctggg tggaagtatc
     3601 gcggtcttca tcatcgtcct catcggcctc ttcgtccttc ccctggccga ggtcctcacg
     3661 ggccagacca tgctcatcat cacgatccct attgcgatcg ccggggcact ctggttcgtt
     3721 ctctccctcc agcggctcgt caaggggaag gggaagaatg ccctgcgcct ccgtatccag
     3781 gccacccggg ccggtatcat cttcatcgct gccgtgttct ggtcggtgct cctcgtcctc
     3841 accctccgga cctcggttct cgatgcctac gactccctcc ctctcgatgc gaagttcctc
     3901 ttctggttcg ggttaaacgg cctcatcgcc atccgggtct acacctccct cctcgcccac
     3961 aaaccctccg aggaggagga acccatcctc gccatcgata atatcaggat agtccagcgg
     4021 tgaggcggac atttgagcca gcggacaggg agtccgggtc catgacctcc cccggcgagc
     4081 gggaattccg gtggaagcag tgcctggtga tccgggggga tatccggatg agctgcggca
     4141 agatctcggc ccaggccgcc cacgcggcgg tcgcggcctt cgagcggtgc gaccgggaga
     4201 cccgggagcg gtggcttgcc gagggacaga agaaggtggt gctgaaggtg aaggacgagc
     4261 ggagcctcct cgagctgaag accattgccg aggcccgggg gatcgcggcg gcgcttatcc
     4321 aggacgcggg cctcaccgag gtcccgccgg ggaccaccac cgccctgggt ctcgggcccg
     4381 cccggagcga ggagatggac cggatcacct ccgaccttcc gctcctatga gggagtcccc
     4441 gtatcccctc gaacagaggc tcgggatttc gtggtacgcg accgggacgg agggtatcgg
     4501 gggatacctc cggggggagc cggaggactt cagggtcgat gagctgccca cgaagtcttg
     4561 ggaggaaagc ggcgagtacc tcatcctccg gctgacgaag cggaactggg agcagcagcg
     4621 cctcgtgaag gatctcgccc ggatctttgg gatcagccac cgcaggatct cgtgggccgg
     4681 caccaaggat cggagggccg tgacctccca gtacatctcc atccatggca tcgcgccgga
     4741 ggccctggag cgaatcaccc tccccggggt caccctcgag gtggctggtc ggtccgacca
     4801 ccctattgcc ctcggagagc tgcgggggaa ccgcttcctc atccggatcc gggaatgcga
     4861 accgacggac cttgccacca gggtggaaaa agtggctggt gcgggtgcgt ccggcttccc
     4921 gaactatgtc ggcatccagc gattcggggt catccgcccg gtcacccacc tggtaggtga
     4981 gagaatcctc cggggcgact tcgagggtgc ggtcctctca tacctggggg catcctttcc
     5041 ctccgaaccg gcagaggtcc gcgaggcccg ggaggcgtac ctccagggaa gggatgcaaa
     5101 ggagtccctc cgccattttc ccgttcgcct gaccttcgag cgggccatgc tccaccacct
     5161 ggacgggcat ccgggtgatt atccgggggc gctccgggcg ctccccccgc gcctcctctc
     5221 gatgttcgtc tcggccttcc agtctttcct cttcaacacc ctcctctccc ggcggtgcgg
     5281 aggggggatt cctcttgacg gacctgcccc gggtgaccgg ctcatcttca cagacgggcg
     5341 ggaggatcgg gtgaccgcgg aaaacctggg ggccgccgtc ctccaggtgc ggcggaaaag
     5401 ggcacagatc gggctgttcg tgcccgggtc gggggtccct gaggcgtcca tcccggacgc
     5461 cgatcccgcg ggggcactcc tcggggagct cgggatcaaa ccggaggact tcgctgccgc
     5521 ctcgaagttc gtccgggtcg cctactccgg cgtgacccgg cccgtggccc tcttgacgga
     5581 catcgatgcc cgtctcgagg agagggacgt cctcctcggg ttctccctgg gcccgggcca
     5641 gtacgcgacc accgtctgcc gggagtttat gaaggctgac ccgatgcgga tgatctgatc
     5701 cccgagccgg cgaggagcct cgcctccccg atggcatcgt ggaggttacc cagccgatcg
     5761 acgaggccga tcttcaccgc ctcctcccct ctcaccaccc tgccgtcctc gatctcggcc
     5821 cgggagacgt tcctctgctc caggatgtcc gcgaggaacc gctcgaagga ggcgttcacc
     5881 agatcctggg cataggcctc ctcccccgct gtcaggttac ggtacgggga ggccatgtcc
     5941 ttccaggcac cggacttcac cacggtcacg ttgtacccct cgttcccgag ccaccggctg
     6001 atgtcggtga agatccagac cgtcccgatc ccgccggtga tggtgtcggg atccgcgaag
     6061 atccggtctg catgggccga aacggcgtac gccgccgagg tcgccatgtc ccccatggag
     6121 accaccaccg gcttccgtgc cttcgcgtac tccatgtcgg cggtgatctc ctgggctgcc
     6181 gcgggggtcc cgcccgggct gttcacccgg aggacgatgg cctcgaccag ggggttgtcg
     6241 gcggcgtccc ggatctcggc ccccacgaac tcgctcccgg tcgcgtcggg gccggagaag
     6301 tcgccggtgg agagggtccc ctccacccgg atgaccgcga cggaactctc cttcgtgtag
     6361 gagaggaacc aggccgccgc gaacacggcg aggaggacga ggaagatgag gccgaggacc
     6421 gcgagccccc gtctccaccc cctcccctcg atcctccgga cctcctcctc gaggaacccc
     6481 atgtcagccg tccccgtaga gcacgtcgta ctcggtgacc aggttggagc cgcacaggta
     6541 gaagcgtttc agggcgagcc ggatcatctc ggactccgag tcgatgtgca tccccccgac
     6601 gagggaaggg gtgtccgccc cgccgacgat gaaggggagg tggatgagcc ggatctcgtc
     6661 cacgagccgg tgccggagca tatgccagtt gagggtcggc cccccctcga tcatgagctt
     6721 cttcaccccg tagtcccgcc agaggatctc catgaggagg gggaggtcca ccttgtcctt
     6781 ccccgcgacg accacatcca cccccttcgc ccggatggcc tccacccggt ccttcggcgc
     6841 ctctcccgcg accgcgatga ccgtgggggc gtctttcttg agcacgttgg catcgagggg
     6901 gatctcggcc ttcccgctcg ggatgacccg gagagggctt tttcccggca ccagccgcac
     6961 cgtgaggaag gagttgtcga tccggatggt gttcgccccg accatgatgg cgtcgtagtc
     7021 tgccctcgtc tcgtgcagga ggatctcggt ctcgggggcc atgtacttca tgaggatctt
     7081 gctcgatgca ccccgcttca gggtgagctt cccgtcggcg gtgatctcgg acatcatgag
     7141 gacatggggt ctctctctcc tcggtggcat ggggtgtcgg cctcgcatat atcttccccc
     7201 ccgatttgaa gaagatttgg gtggagggga acggttttac ggtttcccgc ccttgtggta
     7261 tggatgaaca tcaccgcgct ccggccgagg ctcatctgcc acctcgagcc ggtcgggagg
     7321 gcctttgccc gggcggggtt caccccgaac cagatctccc tcctctccct gctcttcggg
     7381 gtcgcggcca tgttcctctt cgcgacccgg tccttccccg cggggagcct cctcctcctc
     7441 gcctcggggg tgctcgacct cgtggatggc cacgtctccc ggatccagaa ctccgggtcc
     7501 cggttcgggg cggtcttcga ctggatcgtg gacaagtacg tcgacactct ggcccttctc
     7561 ggggtcgggc tctccgggat ccccatcctc acccggatca tcgcggctcc cgccgttgca
     7621 gactccgggg tggtgatggt cgccatcctc ggctccatga tgaacacctt catcaagccc
     7681 gtgacctacg ccgaagccgg attcgcagac cggcatcacg ggaagattga cgaccccctg
     7741 gaaggggtcg gtttcttcgg ccggccggaa acccttctcg tcctcatcgc gggcggcctt
     7801 tcgggcctca tctgggtctc ggtcctcatc atcgccgtcg cgacgaacct ttcagccatg
     7861 gagcggatcg tgtacctccg caggcacctc agtgaatcgt aaaacaggcc tgccgacagc
     7921 cggctaccgg gagccgaggt gggagcagat ctggaccttc agcctcgccc agtacttgta
     7981 atagtcgggc tggtagagat cgatctcccg gctcaggtcc tcgaaacggc tgcactggac
     8041 ctcgcaccgc ttcaggtaga cgcagacctc cagggccgag acggatcgct tcatccggaa
     8101 tggatcatcg ggggccgggg atgatataag tatccgggac ggaaccggac cggtatcctt
     8161 tcatggcagc tggaaatttc atattccggg ccttgtcgta agccgccggt agaggcccgt
     8221 aaggtccccg gaatagacgg tgtacagatc ccccgcccat ctccccagat ccgggatgac
     8281 gacgaagagg gcataggcga tggcgatgag ggcgacgaga gcacctccct cggcgaggat
     8341 gttgtgggcc cagaagaagg attcgtagcc gtattccccg ttggaggcga tctccgggag
     8401 gaagggaaac cactggccgg tgtacgcggc gatgacaaag atattccgga ggaggttcag
     8461 gaggtagatg gtgggaacca cgagaaggaa ggcgaggatc ttctggtggc gggtcgtcgg
     8521 gaccgccccc gccaccccga gcatgatggc catggactgg atgcccgtgc aggcgaggat
     8581 gatctcgatc cggaacccgt tccgaccgat aaggttccag gcgagaagcg ccgccggttg
     8641 gccgacggcg gaaaggaggg agaggatctg cccggtcacc accccgatga ggaagtcccc
     8701 gagggccggc aggtaggcga agggggcata gatgaggaag gccacggccg ccccccggga
     8761 gagggcgagg atggccgggt cctttcggag gagcctccgg ccggtgacca ggagaaaggg
     8821 gagggcgagg atggcaaggg ccgggtagag gatgttattc agggagaggt agtacgggat
     8881 ctcggcgatg aggtagagga ccatggaccc ccacccgagg accgcggcat ggaaacgcca
     8941 cctccagggg aggaggaaga ggaggaaaaa gatcccggag gtgaggacca ggtactcgat
     9001 cattccctga ttgattggct gtacgggaaa aaaagagttc gcaccgaggc gtttgccccg
     9061 gtggctctc
//
LOCUS       DATGDI010000072         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_384635, whole genome shotgun sequence.
ACCESSION   DATGDI010000072 DATGDI010000000
VERSION     DATGDI010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384635"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VKO45_02620"
     CDS             <1..375
                     /locus_tag="VKO45_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04799.1"
                     /translation="RTQSSGNSPVPSQILPLSRAFGEQGKPPALPPGFAARGGRPSPE
                     GIEVMTGFELLLRIKVYRLAADGEKAYNNSIFLYKDNWKDLIRFWQIPTIAPIHKWSH
                     VCWYRLDEREVTELWYFIPQCR"
     gene            348..1226
                     /locus_tag="VKO45_02625"
     CDS             348..1226
                     /locus_tag="VKO45_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HMA04800.1"
                     /translation="MVFHPPVQIVAKAGDAGKYKTGLPWWNMVLRGFMSGAYIAMGGA
                     LATVCSTGIMATDATLRYGTASAGFSQLILGAVFPVGLIITVLTGAELFTGDAMLAPM
                     AAFVHKISWAAVLNLWVWVYIGNFIGSVWYAYLMAYGPFTSWDAAGAATVTAFGSRAI
                     AIAGAKTAYVGTAGMWSAFLKAIGCNWLVNLGILLGICADDLIGKFFGIWFPIMAFVS
                     SGFEHSIANMYFIPAGIFTQGFVTDPTKIVASVNWVSAWTSNLIIVTIGNIVGAMFFV
                     AVIYWVAFRKEIAALK"
     gene            1236..1757
                     /locus_tag="VKO45_02630"
     CDS             1236..1757
                     /locus_tag="VKO45_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HMA04801.1"
                     /translation="MKFRDIELRISPIQIGVLLFFAIFLLLAYWMSRSTMLLIFGIPM
                     VIGLAVIPLLLRHMSQSQYLALVPTYESEAQKVRIKAINMNMLGKPVRIEGVVERVYF
                     DFLSRPQYMIADRSGEISVKMFTAPRERIRKDDIVEVLGTVMKRYVLVGEDPVINCVS
                     IRKVSGGKPPSES"
     gene            1895..2647
                     /locus_tag="VKO45_02635"
     CDS             1895..2647
                     /locus_tag="VKO45_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMA04802.1"
                     /translation="MCETEKEGFIRRGTGLASVGRFRQSVEWLTRALALRPGDPAALV
                     AKASSLSRMGFFQASVRCCEKVIESHPRDPDAWFIRGSVLYMTGRWTEAVPCLEQAAS
                     LDPGHAEAWYTLGNCHYYLGEYEEALCSYDAVIGLDPEYPKAWYNKGVALADVHRYGE
                     ALTCYEECARINPGVAVVYTNMGVALAGLNRFEEAIARFEEAIGINPHDPTPWNNKAA
                     SLRRLGREEEAEACAEKVRDLTRGREIPWIIR"
BASE COUNT          508 a          812 c          809 g          560 t
ORIGIN      
        1 aggacccaat cttcaggaaa tagccctgtc ccttctcaga tcctgccttt gagccgtgct
       61 ttcggggaac agggaaaacc cccggctctt ccccccgggt ttgccgccag gggaggcagg
      121 ccctctccag aagggatcga ggttatgacc ggctttgaac ttttactccg gataaaggtt
      181 tatcgattgg cagcagacgg ggagaaagca tataataact caatttttct atataaggac
      241 aattggaaag atttaataag gttttggcag attcctacaa ttgcacctat acacaagtgg
      301 tctcatgttt gctggtatag gttagatgaa cgtgaggtga ctgaactatg gtatttcatc
      361 ccccagtgca gatagtcgca aaggccggtg acgccggcaa gtacaagacg ggactgccat
      421 ggtggaatat ggttctccgg ggcttcatgt ccggggccta tatcgccatg ggaggcgccc
      481 ttgccaccgt gtgcagcaca ggaatcatgg cgacagacgc gacgctccgg tacggtaccg
      541 cgagcgctgg tttctcgcag ctcatcctgg gagcggtttt cccggtgggg ctgatcatca
      601 cggtgctcac aggcgctgag ctcttcaccg gtgacgcgat gctcgcaccc atggcggcct
      661 tcgtccacaa gatcagctgg gcggccgtgc tcaacctctg ggtctgggtc tacatcggga
      721 actttatcgg ctcggtctgg tacgcctacc tgatggcgta cgggcccttc acgagctggg
      781 atgcggcggg tgccgcgacg gtcacggcct tcgggtcccg tgccatcgcc atcgccggag
      841 caaagacggc gtacgtcgga accgccggca tgtggtcggc attcctcaag gccatcgggt
      901 gcaactggct cgtgaacctc ggtatcctcc tcgggatctg cgccgatgac ctcatcggga
      961 agttcttcgg catctggttc cccatcatgg ccttcgtttc cagcggcttt gagcactcca
     1021 tcgccaatat gtacttcatc cccgcgggta tcttcacgca gggattcgtc accgacccga
     1081 ccaagatcgt ggcttcggtg aactgggtct cggcatggac gagcaacctc atcatcgtca
     1141 ccatcgggaa catcgtcggg gcgatgttct tcgttgcggt gatctactgg gttgccttcc
     1201 gcaaggagat cgcagccctc aagtaagact gcgcgatgaa gttccgcgat atcgagttga
     1261 ggatctcccc gattcagatc ggggtcctcc tcttcttcgc catttttctc cttttggctt
     1321 actggatgag caggagcacg atgctcctca tcttcgggat cccgatggtc atcggccttg
     1381 ccgtcatccc cctcctgctc aggcatatga gccagagcca gtacctggcc ctcgtcccca
     1441 cctacgagag cgaggcccag aaggtcagga tcaaggccat caacatgaac atgctcggga
     1501 aacccgtcag gatcgaggga gtggtcgagc gtgtctactt cgacttcctg tccaggcccc
     1561 agtatatgat cgccgatcga tcgggggaaa tctcggtgaa gatgttcacc gctccacggg
     1621 aacggatccg gaaggatgac atcgtggagg tcctcgggac ggtcatgaag cggtacgtcc
     1681 tcgtcggaga ggacccggtg attaactgcg tctccatcag gaaggtatcc ggcgggaagc
     1741 ccccgtcgga gtcctgatct atccccgcat cccgacagcc cttctgtgaa cccccccttc
     1801 agcgtatctc ccgtcgggcc tgtttttcct tccgccactc cgataggatg gcataagtac
     1861 cggctccacc cacccgttcc ctgaggcggt atccgtgtgc gaaaccgaga aggaggggtt
     1921 catccggagg ggaaccgggc tcgcatcggt gggccggttc cggcagtcgg tggaatggct
     1981 gacgagggcc ctcgccctgc gtcccggcga tcctgccgcg ctcgtcgcca aggcctcgag
     2041 cctctcccgg atgggatttt tccaggcctc cgtccggtgc tgcgagaagg tcatcgagag
     2101 tcatccccgt gacccggatg catggttcat ccgggggagt gtgctctaca tgaccggccg
     2161 gtggacggag gccgtcccct gcctggagca ggcggcctcc ctcgaccccg gccatgccga
     2221 ggcatggtac accctcggga actgccacta ttatctcggg gaatacgagg aggccctctg
     2281 ctcctatgac gcggtcatcg ggctcgatcc cgagtatcca aaggcatggt ataacaaggg
     2341 cgttgctctc gccgatgtgc accggtacgg ggaggccctt acctgctatg aggaatgcgc
     2401 ccggatcaat cccggggtcg cggtggtgta cacgaacatg ggggttgccc tcgccggcct
     2461 gaaccggttc gaggaggcca tcgcgaggtt cgaggaggcc atcgggatca acccccatga
     2521 ccccactccc tggaacaaca aggccgcaag cctccgaagg ctcgggagag aggaggaggc
     2581 cgaggcatgc gccgagaaag tgagggatct cacccggggc cgcgagatcc cctggatcat
     2641 ccggtaggga tccctagaac cggttctcga gcccctcccg gagggatcc
//
LOCUS       DATGDI010000073         6494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_297394, whole genome shotgun sequence.
ACCESSION   DATGDI010000073 DATGDI010000000
VERSION     DATGDI010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297394"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..161
                     /locus_tag="VKO45_02640"
     CDS             12..161
                     /locus_tag="VKO45_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04803.1"
                     /translation="MTYLEKDPVLTGTLGSHIMENLQRITTMEWDSFRTAVHAWEHDQ
                     YLVRY"
     gene            374..1576
                     /locus_tag="VKO45_02645"
     CDS             374..1576
                     /locus_tag="VKO45_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HMA04804.1"
                     /translation="MVLDSGDTAWVLASTALVMLMTPGVGFFYGGMVRKKNIISMIAL
                     SFIVFALVSVQWVLWGYSLSFGTDIGGFVGGLSHLVLSGVGMGPGPYSQTIPGLAYMV
                     FQLVFATVTLAILTSVFAERVKLSSFIVFGLLWTTLVYDPLAHWVWGGGWSAALGALD
                     FAGGTVVHISSGFGALAIAFVIGKRKGFGEYTMEPSNIPMALLGAAMLWFGWFGFNAG
                     SALAANGLAANAFVVTNTAAAAGALAWMCASWYKGRPASLGVVSGAVAGLVAITPAAG
                     YVGPAAAIVIGIVAGLLCFKMLLYRTQKGLDESLDAWSIHGMGGLWGAIATGIFATTA
                     VNNYTGLLSGNVHQFLVQIVAAGASLVYAFVVSYILARIVDATLGLRVREEEEYVGLD
                     ISQHGERV"
     gene            1589..1918
                     /locus_tag="VKO45_02650"
     CDS             1589..1918
                     /locus_tag="VKO45_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HMA04805.1"
                     /translation="MKMVQAIIRPEKLDVVMKNLEERGFIAVTVIEVKGRGEQKGITL
                     EYRGRKIEVDLLPKVKLEVVIRDEDVEAVIGVIKSAAKTGKVGDGKIFVIPVEKMMRV
                     RTDEVWT"
     gene            2535..2912
                     /locus_tag="VKO45_02655"
     CDS             2535..2912
                     /locus_tag="VKO45_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04806.1"
                     /translation="MQQFQKILRKKPVKKMNWLPVLPLLEEGSDPDLKEFMGGIEEDI
                     RAMVGKHYYFIRVGADGPALCRVPLFTRGKFPREIMDHIQHGISDDDLYHAIGMKEGI
                     SSTQGYYKISDHIEGKLRALLDG"
     gene            complement(3065..4258)
                     /locus_tag="VKO45_02660"
     CDS             complement(3065..4258)
                     /locus_tag="VKO45_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nre family DNA repair protein"
                     /protein_id="HMA04807.1"
                     /translation="MMGKGAYLKRISSSARIPSVDVGRDLEGSTPPSVFIGSWNYPKV
                     YAGPMIAPVHGDTRVMDTPEAWIPGAKTQDDILGFRMSLVRGKRPVFIDDLGSGYIGK
                     LQEIALSSGSVESEATFRERPTGFSLSDEHTPYGPSGSLEHFDLGTMKMDRSLERVFD
                     DGDLTAAEAVVSLHSEDIPFSAIQKAFSCGTMGVGKRRRLVPTRWSITACDSTIADHL
                     LSGVKKNPVIDRCLVHESATLNNRYAVLLLPTGWQYEWMEAFLHVMGNEEVVFADHET
                     GAGKTGYSSVGGCYYSCKMAVLEGLARTGRQAGAIVFREAYQGYVPMGVFNVRENVRN
                     AMRSTPQEFEDMKTALAHISGRMKLPLQRFVGESTLLRDQMKTRQTTLSSFAEPKDRV
                     PGPGT"
     gene            complement(4328..4636)
                     /locus_tag="VKO45_02665"
     CDS             complement(4328..4636)
                     /locus_tag="VKO45_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04808.1"
                     /translation="MDYGKILAGNAEPLPIYRNVSLCLENIELFPFILEAIYREAQNF
                     DDETLDRFRFALIRLQVYTDLHHYEDMENTQKVRYVAQVLEKVIFGDLLLEREHPPGD
                     "
     gene            complement(4637..5524)
                     /locus_tag="VKO45_02670"
     CDS             complement(4637..5524)
                     /locus_tag="VKO45_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HMA04809.1"
                     /translation="MPETTAEKEECEKKCETCELECRDRAAPTALPEKAKIHVKHVIL
                     ILSGKGGVGKSTVAVNLAYSLSTHGFQVGILDLDIHGPNIPIMLGIQNERLQAIGNLI
                     EPIRITGNLAAMSMAFLLPDVSTPVIWRGPMKMAAIRQFLEEVNWGELDFLVVDLPPG
                     TGDEALSIAQLAPNVSGVVIVTTPQDVSTVDSRKAVKFVEMLGFRNLGIIENMSGMIC
                     PHCKEEIDLFGKGGGKKAAEEMGVPFLGSIPIDPEMARAGDEGRPFVIKRGETNPTWE
                     AVDRIMEALVTEVEKEAEP"
     gene            complement(5599..6015)
                     /gene="tsaA"
                     /locus_tag="VKO45_02675"
     CDS             complement(5599..6015)
                     /gene="tsaA"
                     /locus_tag="VKO45_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HMA04810.1"
                     /translation="MAGIQLRPLGVISSPFRERGEAPRQGRLEPTESEIQVFPEFAEG
                     LRDLALHPRLFILYWCDRSHRDILTATPPHEGKEHGVFATRSPERPNPIALCLVDLVR
                     LEGNTLTVRGLDALDGSPLLDIKPFYRDLDCPEEGA"
     gene            6106..>6494
                     /locus_tag="VKO45_02680"
     CDS             6106..>6494
                     /locus_tag="VKO45_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HMA04811.1"
                     /translation="MAAGGRIHEDPALRDRAPVFRDRWDAGERLGAYLAGLPALEDPL
                     VCPIPAGGVPVGVAVSRALGCPIRPLIVRKVQIPWNPEAGFGAVSWDGRVFLNRELLG
                     QLHLTPGQVEAAIRKAQANVRERVARFG"
BASE COUNT         1177 a         1956 c         2092 g         1269 t
ORIGIN      
        1 tcgtcgccgc catgacgtac ctcgaaaagg atccggtcct caccgggacc ctggggtccc
       61 atatcatgga gaacctgcag aggatcacga cgatggaatg ggattccttc cggaccgccg
      121 tccatgcctg ggaacacgac cagtacctcg tcagatacta gatctttttt ttctccaccg
      181 gaggacctcc cggtcttcct tccctatttc gttccccacc gattcgggcg gtaaagaaac
      241 tatagataga tttaaatgac atggaaacta acttccttcc tacgatgcat gaggtgcatc
      301 catgaatccc gcgatgagaa gatccgaaag gaaaatatgc cgcccctttg ctaggggacg
      361 gtgaggtggt aaagtggttc ttgacagcgg cgataccgcg tgggtccttg cctccacggc
      421 cctcgtgatg ctcatgaccc ccggcgtggg gtttttctac gggggcatgg tgaggaagaa
      481 gaacatcatc tcgatgatcg ccctctcgtt catcgtcttt gccctggtga gcgtccagtg
      541 ggtcctgtgg ggctacagcc tctccttcgg gaccgacatc gggggcttcg tgggtgggct
      601 ctcgcacctt gtcctctccg gggtcgggat gggtccggga ccctattccc agacgatccc
      661 ggggcttgcc tacatggtct tccagctcgt ctttgcgacg gtgaccctcg ccatcctcac
      721 ctcggtcttc gctgagcggg tgaagctctc ttcgttcatc gtcttcggcc tcctctggac
      781 gaccctcgtc tacgatcccc tggcccactg ggtctgggga ggggggtgga gtgccgcgct
      841 gggggcgctc gacttcgccg gcgggaccgt ggtccacatc agctcggggt tcggcgccct
      901 cgcgatagcc tttgtcatcg ggaagcggaa gggcttcggg gagtacacca tggagccctc
      961 gaacatcccc atggccctcc tgggcgcggc gatgctctgg tttggctggt tcgggttcaa
     1021 tgcgggaagc gcgcttgccg caaacggcct cgccgcgaac gcgtttgtcg tgacgaacac
     1081 cgcagccgcg gcaggcgccc tcgcctggat gtgtgcgagc tggtacaaag gtcgcccggc
     1141 atccctcggg gtggtctcgg gcgccgtcgc cggcctcgtg gccatcacgc cggcggcagg
     1201 gtacgtcggg ccagcggcgg ccatcgtcat cggcatcgtc gcaggccttc tctgcttcaa
     1261 aatgctcctc tatcggaccc agaagggact ggacgagtcc ctggacgcct ggtccatcca
     1321 cgggatgggc gggctctggg gagccatagc aacaggcata ttcgcgacaa cggcggtgaa
     1381 caactacaca ggcctcctct cggggaacgt ccaccagttc ctggtccaga tcgtcgcagc
     1441 aggagcgtcc ctggtgtatg cattcgtggt ttcgtacatc cttgccagga tcgttgatgc
     1501 aaccctgggg ctcagggtgc gggaagagga ggagtatgtc gggctcgata tctcccagca
     1561 cggggagagg gtgtgaggtg gtgattccat gaagatggtc caggccatca tcaggccgga
     1621 gaagctggat gtggtgatga agaacctcga ggagcggggg ttcatcgccg tcaccgtcat
     1681 cgaagtgaag ggccgcggag agcagaaggg gatcaccctg gaataccggg gaagaaagat
     1741 cgaggtcgac ctcctcccca aggtgaagct cgaggtggtg atcagggacg aggatgtcga
     1801 ggcggtgatc ggggtcatca agtccgccgc aaagacaggg aaggtggggg acgggaagat
     1861 cttcgtgatc ccggtcgaga agatgatgcg ggtgcggacc gacgaggtct ggacctgaga
     1921 gagatctttt ttttcctttc ttcccacggg ttccccgccc gcctctcacc cgggggcccc
     1981 ctctggggtt gaccccaccg gccaaaagat cccgggaccc ccgggtcccg gcagagaaca
     2041 tgaggagatg tggcatcgcc gggtgtgaag ggaccagctc cacagggcga tgccacgaac
     2101 ttaagacgac ttccgtggcc ctgccacggc ccccatcgtc agaccgaatg gcatgattca
     2161 tctggggtag aaaggaatta ctcagccata tataataata gtatcctttt ttccatgcaa
     2221 accattatgc ccaccggaaa gctacttcct tccggccaat ccttctgtcc tgcatggacg
     2281 gggtggcaac ccacatatcc ggaggccacg gggatggtgc tccggcaccg ggcgatcgtt
     2341 tctttctgca ggcagggcgg gcggagaagg atccaggggg gatactccat agagggccgg
     2401 aatccatcgg gaccgggtta ccctggtccc cggggtttct ccatccggcc tggcaggatg
     2461 gttcgggtgg ttgcccgcgg gccaggccgg ggagagggta tggatgctga acatcccagg
     2521 agagaggtga aaagatgcaa cagttccaga agatcctcag gaagaagccg gtgaaaaaaa
     2581 tgaactggct cccggtcctt cccctgctcg aggagggatc agatccggat ctcaaggaat
     2641 tcatgggagg gatcgaggag gatattcggg caatggtagg gaaacactac tacttcattc
     2701 gggtgggtgc agacggaccc gccctctgca gggtccccct cttcaccagg gggaagttcc
     2761 cgagagagat catggaccac atccagcacg gcatctcgga tgacgacctc taccatgcca
     2821 tcgggatgaa ggaagggatt tcctcaaccc agggatacta caaaatctcc gaccatatcg
     2881 aggggaagct ccgggccctc ctcgatggct gaacacagtt caaatccacc atttttttcc
     2941 tgatccgagc tgggaatcgg tttcatcctg acggcggtac ggagcgtcgc gggtacgaac
     3001 ctcagggcca gggtgcgggg ggcgggaagc gcgatggacc ggcagtctta cagacttggt
     3061 cctcttacgt gccgggaccg ggaacacggt ccttcggctc agcgaaggag gaaagggtcg
     3121 tctgccgtgt cttcatctgg tcccggagca gggtgctctc cccgacaaac ctctggaggg
     3181 ggagcttcat ccggccggaa atgtgagcaa gggccgtctt catgtcctcg aactcctggg
     3241 gggtgctcct catggcgttc cggacgttct cccgcacgtt gaagaccccc atggggacat
     3301 acccctggta cgcctcccgg aacacaatgg cccctgcctg cctcccggtc cgtgcgagtc
     3361 cctccaggac cgccatcttg caggagtagt agcatccccc gaccgatgag tatccggtct
     3421 tccctgcccc ggtttcatgg tcggcgaaaa ccacctcctc gttccccatg acatggagga
     3481 aggcctccat ccactcgtac tgccacccgg tgggaaggag gaggaccgca taccggttgt
     3541 tcagggtggc cgactcgtga acgaggcacc ggtcgatgac cgggttcttc ttcactccgg
     3601 agaggaggtg gtcagcgatg gtgctgtcgc aggcggtgat ggaccaccgg gtggggacga
     3661 gccgccgcct cttcccgact cccatcgtcc cgcaggagaa ggccttctgg atggccgaga
     3721 aggggatgtc ctccgagtgg agggagacca ccgcctcggc cgcggtgaga tctccgtcgt
     3781 caaagacccg ttccaggctc ctgtccatct tcatggtccc gaggtcgaag tgttcgaggg
     3841 agccgctcgg tccgtagggg gtgtgctcgt cagagaggga gaagcccgtc ggtctctccc
     3901 ggaaggtcgc ctcgctctcg accgagccag aggacagggc gatttcctgg agcttcccga
     3961 tgtacccgga cccgaggtcg tcgatgaaca cggggcgctt gccccggacg agactcatcc
     4021 ggaagccgag gatatcatcc tgggtcttcg cccccgggat ccatgcctcg ggggtatcca
     4081 tgaccctcgt gtccccgtgg acgggtgcga tcatgggacc ggcatagacc ttcgggtagt
     4141 tccaggaccc gatgaaaacc gacgggggag tggagccttc caggtctctc ccgacgtcaa
     4201 cggaggggat acgggctgag gaggagatcc tcttcaggta ggcccccttg cccatcatca
     4261 cctgtacgtc ggtggtggtt ccttaaaaaa ggatcagggc cgggtcgaaa gtgttcccgg
     4321 ctgcccttca gtctcccggc gggtgctccc gctccaggag caggtccccg aagatgacct
     4381 tctcgaggac ctgggcgacg taccggacct tctgggtatt ctccatgtcc tcgtagtggt
     4441 ggaggtcggt gtacacctgg agacggatga gggcaaaccg gaaccggtcc agggtctcgt
     4501 cgtcaaagtt ctgtgcctcc cggtagatcg cctcgaggat gaaggggaag agctcgatgt
     4561 tctcgaggca gagggagacg ttccggtaaa tggggagggg ctcggcgttc ccggcgagga
     4621 tctttccgta gtccatctag ggctccgcct ctttctccac ttccgtcacg agagcttcca
     4681 tgatccggtc caccgcctcc caggtggggt tcgtttcacc ccgcttgatg acgaagggcc
     4741 tcccctcgtc ccctgccctg gccatctcgg ggtcgatggg gatggacccg aggaagggga
     4801 cccccatctc ctcggcggcc ttcttcccgc ctcccttccc gaagaggtcg atctcctcct
     4861 tgcaatgggg acagatcatg ccgctcatgt tctcgatgat cccgagattc cggaacccga
     4921 gcatctccac gaacttcacg gccttccggg agtcgacggt agagacatcc tgcggggtcg
     4981 tcacgatgac caccccggag acgttcgggg cgagctgggc gatggagagg gcctcgtccc
     5041 cggtccccgg gggaaggtcg acaacgagga aatcgagctc gccccagttc acctcctcga
     5101 ggaactgccg gatggcggcc atcttcatcg ggccccgcca gatgaccggg gtggagacgt
     5161 ccgggaggag gaacgccatg gacatggcgg cgaggttccc cgtgatccgg atcggttcga
     5221 tgaggttccc gatggcctgc aggcgctcgt tctggatccc gagcatgatg gggatgttcg
     5281 ggccgtggat gtccaggtca aggatcccca cctggaagcc gtgtgtcgag agggaatagg
     5341 ccaggttcac ggccacggtg gactttccca ccccgccctt cccggagagg atcaggatca
     5401 cgtgtttcac gtggatcttt gccttctccg ggagagccgt cggcgccgca cggtcccggc
     5461 actcaagctc gcatgtctcg cacttcttct cgcactcttc cttctctgcg gtggtttcgg
     5521 gcatatcctc cctcacagat gacgaactgc ggcataaagc tgtcccctga aagtcataaa
     5581 ggtatgagat atggcctctt acgccccttc ctccgggcag tcgaggtccc ggtagaaggg
     5641 cttgatgtcc aggagagggg atccgtccag ggcgtccaga ccccggacgg tgagggtgtt
     5701 tccctcgagc cgcacgagat ccacgaggca gagggcgatg gggttcggcc tctcgggaga
     5761 tcgggtcgca aagaccccgt gctccttccc ctcgtggggc ggggtggcgg tgaggatgtc
     5821 ccggtgagaa cggtcacacc agtagaggat gaagagccgg gggtggaggg cgaggtcccg
     5881 gagtccctcg gcaaactccg ggaaaacctg gatctcgctt tccgtcggtt cgagccgccc
     5941 ctgcctgggt gcctcccccc tctccctgaa cggggatgaa atgaccccga ggggccgaag
     6001 ctggatccct gccatgggag gaggatgcac ccggaacgag atgaaggtac cgctggggcc
     6061 ccgggcatat cttcaagata tccgccgcga tagggatcgc cgttgatggc tgcaggaggg
     6121 cgaatccatg aagacccggc ccttcgcgac cgggcacccg tgttcaggga tcggtgggat
     6181 gccggagaac ggcttggtgc ctatctcgcc ggtctccccg ccctcgaaga tcccctggtc
     6241 tgcccgatcc ccgccggcgg tgtccccgtt ggcgtcgcag tttcccgggc cctcggttgc
     6301 cctatccgtc ccctcattgt ccggaaagtc cagatccctt ggaaccctga ggccgggttc
     6361 ggggccgttt cctgggatgg ccgggtcttc ctgaacaggg aactcctggg ccagctccac
     6421 ctcactcccg gtcaggtcga ggccgcgatt cggaaggcgc aggcaaacgt cagggaacgg
     6481 gtggcacgct tcgg
//
LOCUS       DATGDI010000074         1794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_355612, whole genome shotgun sequence.
ACCESSION   DATGDI010000074 DATGDI010000000
VERSION     DATGDI010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355612"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VKO45_02685"
     CDS             <1..182
                     /locus_tag="VKO45_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HMA04812.1"
                     /translation="APIASWVGENDEDTTKDWDQIERNKYTQEDLQNMIDSTLRLAYN
                     LKRGAGKGEEPSGTP"
     gene            complement(179..1663)
                     /locus_tag="VKO45_02690"
     CDS             complement(179..1663)
                     /locus_tag="VKO45_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04813.1"
                     /translation="MPDLHRKSPSGIFSNRVLTNSTLRKPPLGIFTAMLKEEWRIHST
                     MFGSAGFALFPLFLVFLAAMAALFWPVFADVLPARTAVTLLHAGYLLAGMSVGGFGLF
                     GREVMNRRFGQASLIAYSSRSLPVSEQSILANFLLKDTVYYLFFWVFPFVAGLAIAAP
                     WIGLPLVSVLALLATLSLSFLLGLSAVFLLSTIYVHSPRALLGLFIAGAAGALLYART
                     LDAGVLGLLPSLGLYYGPTPALLLATLLLIVVPSAISVRFLKIEYPEETRRLPAALDR
                     FTGMLSWTPDAHFIAKDALDLSRSEGGAGKIVFSFLFPVAFIWIALFILLRFIPALSR
                     IMVFAILLGVVSSTIYNWVTEFDLFTSYAFLPVGAGTVIRGKLESYGLLNLVSVAILV
                     AAGLGTGQVELLLPALLVFASVSFYAVAVTVYVGGLSPSVMIYNAKVYVPYAGLITPA
                     LLFLLFVSLPNPWFALSGLCLIPAGWFILGKAFSRWNAVEQPGF"
BASE COUNT          419 a          501 c          665 g          209 t
ORIGIN      
        1 aggcccccat cgcctcctgg gtcggcgaga acgacgagga cacgaccaag gactgggacc
       61 agatcgagag gaacaagtac acccaggagg atctccagaa catgatcgac agcactctca
      121 ggctcgccta taacctgaaa aggggggcgg ggaagggcga ggaaccgtct ggcactccct
      181 aaaatcccgg ctgctccacc gcgttccacc gggagaatgc cttccccagg atgaaccacc
      241 cggcgggaat caggcacagg cccgagaggg caaaccacgg gttcggcagg gagacgaaga
      301 ggaggaagag gagcgccggc gtgatgagcc ccgcgtaggg aacatagacc ttcgcattgt
      361 agatcatgac cgacggggag agcccgccca cgtacacggt gaccgctacg gcgtagaagg
      421 agaccgaggc gaagacgagg agggcgggga ggaggagttc cacctgcccc gtcccgaggc
      481 ccgcggcgac gaggattgcc accgacacga ggttgaggag cccgtagctc tcgagctttc
      541 cccggatgac ggtccctgcc ccgacgggca ggaaggcata ggaggtaaag aggtcgaact
      601 cggtgaccca gttgtagatg gtggaggaga ccaccccgag gaggatggca aagaccatga
      661 tccgggaaag agccgggatg aaccggagga ggatgaagag ggcgatccag atgaacgcga
      721 ccgggaacag gaaggagaag acgatcttcc ctgccccgcc ctcgctccgg gagaggtcga
      781 gggcgtcctt cgcgatgaaa tgggcgtcgg gcgtccatga gagcatcccc gtgaaccggt
      841 ccagtgccgc cggcaagcgc ctcgtctcct cgggatactc gatcttcagg aaccggaccg
      901 agatggccga gggcacgacg atgaggagga gagtggcgag gaggagggcg ggagtcggcc
      961 cgtagtagag gccgagggag ggaaggagac cgagcacccc cgcatccagc gtgcgggcat
     1021 agaggagagc gcctgcggcg ccggcaatga agaggccgag gagggcccgg ggggagtgta
     1081 cgtagatggt cgagaggagg aagaccgccg agaggccgag gaggaaggag agggagaggg
     1141 tggcgaggag ggcgaggacc gagacaaggg gcaggccgat ccagggggcc gcaatcgcaa
     1201 ggcccgcgac gaaggggaag acccagaaga agaggtagta gacggtgtcc ttcaggagga
     1261 agttcgcgag gatggactgc tcggagaccg ggaggctccg ggaggagtac gcgatcaggg
     1321 atgcctggcc gaaccggcgg ttcatcacct cccggccgaa gagcccgaag ccccccacgc
     1381 tcatcccggc gaggaggtag ccggcatgga ggagggtcac cgccgtccgg gcgggcagga
     1441 cgtcggcaaa gaccggccag aagagggcgg ccatggcggc gagaaagacg aggaacaggg
     1501 gaaagagggc aaagcccgcc gaaccgaaca tggtggagtg gatccgccac tcctccttca
     1561 gcatggcggt gaagatcccc agcgggggtt ttcgaagggt cgagttcgtg agaacgcgat
     1621 tcgagaagat ccccgaaggg gattttcgat ggaggtcagg catcctgcct ctccccgcgg
     1681 gtggccgaga ggaagacatc ctccaggctc ctcgaggatc ccctcaggtc tgccaccgtc
     1741 ccctcccgga ccagatcccc ccggtggata atcccgatcc tcgtgcagat ctcc
//
LOCUS       DATGDI010000075         3265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_6638, whole genome shotgun sequence.
ACCESSION   DATGDI010000075 DATGDI010000000
VERSION     DATGDI010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6638"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VKO45_02695"
     CDS             complement(<1..477)
                     /locus_tag="VKO45_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA04814.1"
                     /translation="MKTAESVMVKVPVLSLTDHVTRARKILRDDEFREVYVQDEKRHL
                     AGYLDITDALRITDTRSNVTVEGFLKDAARVSPGDPLEKVAAVIRDAKTDSAAVVDGQ
                     GTVLGAVVLSGIFPILITRHELRGTVGDWMSRGVVTIDSDEMIQKVYTLIMESGFTA"
     gene            complement(474..1037)
                     /locus_tag="VKO45_02700"
     CDS             complement(474..1037)
                     /locus_tag="VKO45_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA04815.1"
                     /translation="MSPKRPTIQFETRVPLREVMRYPPTTIDSDASVADASLAMCRDE
                     VGSCIVLENNFPIGIITEEDINCKVVAKDRKPSDVNVKDAMSSPLITIGAGKSVGDAA
                     KMMVKRKVRRLPVVEGQKVVGIVTVRDILSVASAMEEILSELIDVNREEVLEMGVCDR
                     CGSMSDDLRRSDTLMLCPACRTEDLLV"
     gene            1268..1909
                     /locus_tag="VKO45_02705"
     CDS             1268..1909
                     /locus_tag="VKO45_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Era-like GTP-binding protein"
                     /protein_id="HMA04816.1"
                     /translation="MSIFVRTKKKLSGFLRIFFKKRRARIGIYGPPNAGKTTLANRIV
                     RDWTGDAMGAVSEIPHETRRALRKENITITGVNGDSVDIDIVDTPGVTTKIDYHEFMN
                     FGLSKEEAVTRAREATEGVAEAMHWLREDIDGVIYMLDATLDPFMQVNIMMVGIIESR
                     KLPVIIVANKIDLPEAAPNRIRSAFPQHPVVPISSLDGSNVEQLYEIMTEKFG"
     gene            1915..2283
                     /locus_tag="VKO45_02710"
     CDS             1915..2283
                     /locus_tag="VKO45_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2073 domain-containing protein"
                     /protein_id="HMA04817.1"
                     /translation="MQGIQIDLISDERLARLTPMEKIRLILDDVKKGNIVVLEKGLPP
                     EEASKLIEITMMEIMPDGFTGIEMETYPAREAAEGFSGIFSRFVGRRPESRLTVIGPA
                     NQIKMIKKEKDLISAWVSSR"
     gene            2289..2714
                     /locus_tag="VKO45_02715"
     CDS             2289..2714
                     /locus_tag="VKO45_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing protein"
                     /protein_id="HMA04818.1"
                     /translation="MPHKCTKCGREFKDGSTDILKGCPSCGSKKFLYIREEHRNRDVL
                     EEKTIQEIATETKEEVLEVVGSPETATDPRGRVESIRILSPGNYELNLKKLAESGDMV
                     VGVGKDAKYLLDISSMSKPEEKPEKGKKGKKEKGKGRKK"
     gene            complement(2826..>3265)
                     /locus_tag="VKO45_02720"
     CDS             complement(2826..>3265)
                     /locus_tag="VKO45_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HMA04819.1"
                     /translation="KTNDLLTGRGWVMGIQTFKLYPELWNAKATPNYYTTFPSGSIST
                     ATWTYLAFTFKKNDVMRGYVNGQQVNSTNVNNNNIGTATMNLTIGAEPWNAPPTQWFA
                     NARIDEVRLSNLVRSAPWISTGYNNTYSPGTFHYPGSQLGWTC"
BASE COUNT          675 a          943 c         1056 g          591 t
ORIGIN      
        1 ggcggtgaag ccgctctcca taatcagggt gtagaccttc tggatcatct cgtcggagtc
       61 gatggtcacc acgccccggc tcatccagtc gccgacggtc ccccggagct cgtgccgggt
      121 gatgaggatg gggaagatcc cggagaggac cacggctccg agcacggttc cctgcccgtc
      181 gaccacggcc gcgctgtcgg tcttcgcgtc ccggatgacc gccgccacct tctccagggg
      241 atccccgggg gagacgcgcg ccgcgtcctt caggaacccc tccaccgtga cattggaacg
      301 ggtgtcggtg atccggaggg catcggtgat atccaggtat ccggcgaggt gtctcttctc
      361 gtcctggaca tagacctccc ggaactcgtc gtcgcggagg atcttccggg cccgggtcac
      421 gtggtcggtg agggagagga ccggcacctt caccatgacg ctctcggcgg ttttcatacg
      481 aggagatcct ccgtcctgca ggccgggcag agcatgaggg tgtcgctccg gcggagatcg
      541 tcggacatgg agccgcaccg gtcgcagacc cccatctcga ggacctcctc ccggttcacg
      601 tcgatgagct ccgagaggat ctcctccatg gcgctcgcga cggagaggat gtcccgtacg
      661 gtgacgatgc cgacgacctt ctgcccctcg accacgggga gcctcctcac cttccgcttc
      721 accatcatct tggccgcatc ccccaccgat ttcccggcgc cgatggtgat caggggggag
      781 gacatggcat ccttgacatt gacgtccgag ggcttcctgt ccttcgccac gaccttgcag
      841 ttgatgtcct cctcggtgat gatcccgatg gggaagttgt tctcgaggac gatgcagctc
      901 cccacctcgt cgcggcacat ggcaagggag gcgtctgcca ccgatgcatc tgagtcgatg
      961 gtggtggggg gatatcgcat cacctcccgg agggggacgc gagtctcgaa ctggatggtc
     1021 ggccttttcg gggacatagg aattcctgag tggattgaaa cggatgtttc cctgtaattc
     1081 ctgaaagttc actcttacta ggtgaaggca gtataaaaga attcttctcc ccgtggaaat
     1141 cgcgcgacga tacggggcgg aacgacggga tgcggagggg gagcgcacgg aggattatat
     1201 atgcaggaaa agagagaatt ctcttcaaga atcccatcag gaataccacc aggggggaat
     1261 gcgcgacttg agcatctttg tcaggacgaa gaagaagctc tcgggttttc ttcggatctt
     1321 cttcaagaag aggagggcga ggatcgggat ctatggcccg ccgaacgcgg gcaagacgac
     1381 cctcgcgaac cggatcgtga gggactggac cggggacgcc atgggggcgg tctccgagat
     1441 cccccacgag acaaggcggg ccctccggaa ggagaatatc accatcacgg gggtgaacgg
     1501 ggactccgtg gacatcgata tcgtcgacac acccggcgtg accacgaaga tcgactatca
     1561 tgagttcatg aacttcggcc tctccaagga agaggcggtg acccgggccc gggaggccac
     1621 cgagggggtc gccgaggcca tgcactggct ccgggaggat atcgacgggg tcatctacat
     1681 gctcgacgcg accctcgacc ccttcatgca ggtgaacatc atgatggtcg ggatcatcga
     1741 gtcgaggaaa ctccccgtca tcatcgtcgc gaacaagatc gacctccccg aggcggcccc
     1801 gaaccggatc cggagcgcct tcccccagca cccggtggtg cccatctcga gccttgacgg
     1861 gtcgaacgtg gagcagctct acgagatcat gaccgagaag ttcgggtgac ggcgatgcag
     1921 gggatacaga tcgacctcat atccgatgaa cggctcgccc gcctcacccc catggaaaag
     1981 atccggctca tcctcgacga cgtgaagaag gggaacatcg tggtcctcga gaagggcctc
     2041 ccgcccgaag aggcgagcaa gctcatcgag atcacgatga tggagatcat gccggacggg
     2101 tttaccggga tcgagatgga gacctatccg gcgagggagg cggccgaggg cttctcgggc
     2161 atcttctccc ggttcgtggg aaggcgcccc gagtcccggc tcacggtcat cgggccggcg
     2221 aaccagatca agatgatcaa gaaggagaag gacctcatct cggcctgggt ctcctcgcgg
     2281 taaggcagat gccccacaag tgcacgaaat gcgggcggga gttcaaggac ggctccaccg
     2341 acatcctgaa gggatgcccg agctgcggga gcaagaagtt cctctacatc cgggaggagc
     2401 accgcaaccg cgacgtgctc gaggaaaaga ccatccagga gatcgctacc gagacgaagg
     2461 aggaggtcct cgaggtggtg ggatccccgg aaacggcaac ggacccccgg ggccgggtcg
     2521 agtccatccg cattctctcc ccggggaact acgagctgaa cctgaagaag ctcgccgaat
     2581 cgggggacat ggtggtcggg gtcgggaagg acgcgaagta cctcctcgac atctcctcca
     2641 tgtcaaaacc ggaggagaag ccggagaaag ggaaaaaggg gaagaaggag aaggggaaag
     2701 gcaggaagaa ataggatcct ggacccttcc ccttcgatag ggaatccccg atcaagattc
     2761 ccggaatccg ggcctgcgtg aggatgcgtg cgggcgaaag aaaaagtcgg agctctcccc
     2821 tcttcctagc atgtccatcc gagctggctc cctgggtagt ggaaggtgcc gggactatat
     2881 gtattgttgt acccggtcga gatccacggg gccgaccgga cgaggttcga cagccgcacc
     2941 tcatcgatac gggcgtttgc gaaccattgg gtaggagggg cgttccatgg ctcggccccg
     3001 atggtgaggt tcatggtcgc tgtgccgatg ttgttgttgt tgacgttggt gctgttcacc
     3061 tgttggccat tcacgtaccc tcgcatcacg tcgttcttct tgaaggtgaa ggcaagatag
     3121 gtccaggtag ccgtcgagat gctcccggaa ggaaaagttg tgtaatagtt cggagtcgcc
     3181 ttggcgttcc agagctcggg atacagtttg aaggtctgga tgcccatcac ccagccgcgc
     3241 ccggtcagga ggtcattcgt cttgc
//
LOCUS       DATGDI010000076         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_152398, whole genome shotgun sequence.
ACCESSION   DATGDI010000076 DATGDI010000000
VERSION     DATGDI010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152398"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /gene="pseB"
                     /locus_tag="VKO45_02725"
     CDS             complement(<1..864)
                     /gene="pseB"
                     /locus_tag="VKO45_02725"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HMA04820.1"
                     /translation="MDYSLSGKTILLTGGTGSFGQKFTEIVLGDHSPKALRIFSRGEL
                     LQIKMEERFRDERLRFFIGDVRDRARLHRAMRGADVVVHAAALKHIPACEYNPIEAIK
                     TNIDGTGNVIDAAIDAGVDRVILISTDKAVHPVNLYGATKMVAEKLIIQGNSYTGGIK
                     TRLSCVRYGNVIGSRGSVIPVFLDQKKTGIITITDSRMTRFWITLDQGVRFVVRAIGR
                     MEGGEIFVPKIPSMRLMDLADAIAPHAEKNFSGIRPGEKLHEVLITEDEARHTREYED
                     HFVVEPEFPFWR"
     gene            1268..>2869
                     /locus_tag="VKO45_02730"
     CDS             1268..>2869
                     /locus_tag="VKO45_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04821.1"
                     /translation="MLETDMRELHGIPSLKPARYSMYSLKMLLDVLYTSIIVTHRILD
                     EEHPDTICIPVKHSEDAVSRHGFSNEESVFARILSFDGWSVPVEFLGPETSILSGRKT
                     TVGKISPGGYLRGWIRSFGPVMNIGLLAKHRKILSASGAFLGGLPGRSVGPVLIQGNG
                     YNWDDALVELYRKGWRPVHRLDDEVAGSPPASASTQKSIHDIVAAHPQLQEFRRFLSI
                     DTGPFLSERISTIVSDSFVESIASYRRTRDVIAKKGIRALLVSILSRPGDHAAVQAAR
                     DSGIPVISWQHGGSGYTFHPMMPNLECAHSDLHLVFGTPVAEKYREAMEWLGWEHRPP
                     VVPVGSSTLDRIREMLPGGGPHGKGPVLYVTTAYLQNNFYISQPQQVIGWDEHLWQVQ
                     RGVIDLAGRFPGLEFIIKLHPSQSSREPLGSYIRDSGISNIRCISNEARLDDLLRRCS
                     VLLFDLVSTGILQAMLTKKPVFLYSGLFRLDGEALELLRRRAMVSEDPDDFIGRFSGY
                     LDGDLEARELADSLDFLGAYGLHTCD"
BASE COUNT          564 a          863 c          801 g          641 t
ORIGIN      
        1 tctccagaag ggaaattccg gttcaacgac gaagtggtcc tcatactcac gcgtgtgcct
       61 ggcttcatcc tcggtgatga gtacctcgtg gagcttctcg ccgggccgta tccccgagaa
      121 attcttctcc gcgtgaggag cgatggcatc cgcgaggtcc atcagcctca tggacgggat
      181 cttcgggacg aagatctccc cgccctccat cctgccgatg gccctgacca cgaaccggac
      241 cccctggtcg agggtgatcc agaaccgggt catccgggaa tcggtgatgg tgatgatgcc
      301 ggtcttcttc tgatcgagaa aaaccgggat cacgctcccc cggcttccga tgacattgcc
      361 gtacctgaca cagctgagcc gggtctttat cccgcctgta taggagttgc cctggatgat
      421 cagcttctct gccaccatct tcgtcgcccc gtagaggttc accggatgca ccgccttgtc
      481 ggtggagatc aggatgaccc ggtcgacccc tgcatcgatg gcggcatcga tgacattccc
      541 ggtcccgtcg atattggtct tgatcgcctc gatggggtta tattcgcagg cagggatgtg
      601 ttttaaggcc gcggcatgga ccaccacatc cgcgccccgc atagccctgt gcagcctcgc
      661 tcggtcccgc acatcaccga tgaaaaaccg gaggcgctca tcccggaacc tctcctccat
      721 tttgatctgg aggagttccc cccgggagaa gatcctgaga gccttcgggg aatggtcacc
      781 gaggacgatt tcggtgaact tctggccaaa ggatccggta cctccggtga gcagtatggt
      841 ctttccggac agggaatagt ccatatcaga agtttggtga gtcgtattat tagttcttgg
      901 gatactcctg caggggaacc cggcccgatc ccgtccaaat gccttgctgg ggagggaatt
      961 ttcccggatc tctcgttgaa taaccatggg ttttaatgga ctggagagcc ctagtttgaa
     1021 ctatcccggg ggggaagaaa acatccggtc catcgttctc ctcgaagatc cctccgatat
     1081 ccctgcggct ctttcccgag cggggaagca tggtggcgat gccatcttca tctccctctc
     1141 cccttcggcc tcctatggtc tcgatcgacg aaaaatacct caccgtagta tgctctccta
     1201 cggacgtgga gaggacagct accgcacggg actggataac tttaaaagac tggacaattt
     1261 attccgtgtg cttgagacgg atatgaggga gctgcatgga attccctccc tgaaacccgc
     1321 acgctactcc atgtattccc tgaagatgct ccttgatgtc ctctatacct ccatcatcgt
     1381 cacccaccgg atccttgacg aggaacatcc cgataccatc tgcatcccgg tcaagcattc
     1441 tgaggacgcc gtaagccggc acggcttcag caacgaggaa tccgtttttg cgaggatcct
     1501 ttccttcgac gggtggagtg tacctgttga atttctgggt ccggaaacgt ccatcctctc
     1561 cggaaggaaa acgacggtgg gcaagatctc ccccggagga tatcttcgcg gctggatcag
     1621 atccttcggc ccggttatga atatcggact ccttgccaaa caccgaaaaa tcctttctgc
     1681 ctcgggagcc ttcctcggtg gtcttccggg tagatccgtc gggcccgtac tgattcaggg
     1741 gaacgggtac aactgggacg atgccctcgt cgagttatac aggaagggct ggagacccgt
     1801 ccaccggctc gacgatgagg ttgccgggag ccctccggcg tccgcgagta cccagaaaag
     1861 tatccatgat atcgtggcag ctcacccgca actccaggaa ttcaggcgat tcctttccat
     1921 cgataccggg cccttcctgt ccgagcgcat cagcaccatc gtatccgatt cctttgtcga
     1981 atccatcgca tcctatcgcc gcacgcgcga cgtgatcgcg aagaaaggga tacgagccct
     2041 tctcgtctcg atcctgagca ggccgggcga ccatgcagcg gtccaggctg cccgggattc
     2101 cggcattccg gtcatctcct ggcagcacgg agggtccggg tataccttcc accccatgat
     2161 gcccaacctc gaatgcgctc actccgatct ccatctcgtc ttcgggaccc ctgtggcaga
     2221 gaaatacagg gaggccatgg agtggctggg atgggaacac cggccccctg tcgtgcccgt
     2281 gggctcaagc acccttgacc ggatacggga gatgctgccg gggggcggcc cccacgggaa
     2341 gggcccggtg ctctatgtca ccaccgccta cctccagaat aacttctaca tctcccagcc
     2401 tcagcaggtg atcggctggg atgagcacct gtggcaggtg cagagggggg tgatcgatct
     2461 cgccgggagg tttcctggcc ttgagttcat catcaagtta cacccgagcc agagttcgag
     2521 ggagccactc ggatcgtaca tccgtgattc aggcatttcg aatatccggt gcatatcgaa
     2581 cgaggccagg ctggatgatc tcctgcggcg ttgctccgtc cttctctttg atctcgtatc
     2641 cacgggtatc ctccaggcaa tgcttacgaa aaagccggta ttcctttact ccggtctctt
     2701 ccggctcgat ggtgaggccc tggaattgct ccgaaggcgg gccatggtgt cagaggaccc
     2761 cgacgacttt atcggaaggt tttcaggata tctcgatggg gatctggagg ccagggagct
     2821 tgcggattcc ctggattttc tcggggcata cggcctgcac acctgcgac
//
LOCUS       DATGDI010000077         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_355850, whole genome shotgun sequence.
ACCESSION   DATGDI010000077 DATGDI010000000
VERSION     DATGDI010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355850"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..617)
                     /locus_tag="VKO45_02735"
     CDS             complement(78..617)
                     /locus_tag="VKO45_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HMA04822.1"
                     /translation="MPPHCDTMDGPVVTAAKKALETGNVNLVLIWVPEEADGEVRRAF
                     ERAIQARKGGQAAWEIADEWFFETVVRLHRAGEGAPYTGLKPSGLDEGPVVPRAEKAI
                     DTGDPKETIRFICHTLEDDLKHRFHRVMETKRYDVNDVAAGRAYIQAYIGFVVHAHHL
                     YAGIVGGSGHGERGGGHGH"
     gene            complement(780..1184)
                     /locus_tag="VKO45_02740"
     CDS             complement(780..1184)
                     /locus_tag="VKO45_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMA04823.1"
                     /translation="MTKTSSPKPAQVTYHRIYADALGGSHLDVVTVKQSLAQAAPPAA
                     PFYVSEDKPASKYRFYSFEPGWIGELHPAPTRQFLALMSGAVEVETTDGTVRRFGPGD
                     LVLLEDTSGRGHVTRNIGDGYAMFLVVPVPAT"
     gene            complement(1226..1516)
                     /locus_tag="VKO45_02745"
     CDS             complement(1226..1516)
                     /locus_tag="VKO45_02745"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HMA04824.1"
                     /translation="MTIYITLGNLSRDAFEKIDNLEERDRKAKNLIESLGGKLISLHY
                     TFGRYDFVAMMDMPSKESVVKFLSILGKYGTVQTETLETIPAEMLYKIAKEV"
     gene            1697..3229
                     /locus_tag="VKO45_02750"
     CDS             1697..3229
                     /locus_tag="VKO45_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HMA04825.1"
                     /translation="MKGKGYFIVVLCFVLAVAFTCGCSTQGPSAGTGVSPTPTGPATD
                     AGLRIVTEDFPPFNYAGPDGKAAGLSTDVVNAILARLNQKAAIEVMPWTGGYQLALSG
                     PRVVLFSTGRTDERETLFKWVGPIAANDYTLYARKGSALQIGSLEAAKKAGLIGVVKD
                     DARYQFLLENRFGNILTCDDETGCIRNLTAGTTGLLLGSSLSTPDIARKAGIDPSSLD
                     ALYTIRTVPLYIAFSRDTPDSVIAAWQGALDAMKRDGSFDAILAKYGMTPSGITVSQS
                     TSEAQAGLALEIVAAETDGQLKAVLRPLEVLAATTEAQSGGWQNIRPLLAVLEEKEPA
                     ILAWYALPDGSYYTVDDGLTSANLKNRSYFPVVLAGNESVGIVVVSKATGRNVAVVAV
                     PVKAGGGVTGVLGSSVYLDTLTDTLREEIPAPFVFYAIDTEHKFALHSEKGQITRNIA
                     TIGPGSSFGQAISTILAGDSGTVEYDDGGIHYTARFSSDPLTGWRLVVAWPKAGSTTT
                     TT"
BASE COUNT          626 a         1011 c         1040 g          663 t
ORIGIN      
        1 ggagaactgt atgcaacacc ggaggtaaac ccttccggtg ggtcctcgaa aggtgaaatg
       61 catgaaaact ccatctctca gtgcccgtgc ccacctcccc tctccccgtg cccgctcccg
      121 ccgactatcc ctgcgtagag atggtgggca tggacgacaa acccgatgta cgcctggata
      181 tatgcccgtc ccgcggcaac gtcgttcacg tcgtagcgct tcgtctccat gacccggtgg
      241 aaccggtgct tcaggtcatc ctccagcgtg tggcagatga aacggatggt ctccttcgga
      301 tccccggtgt cgatggcctt ctcggcccgg gggacgaccg ggccctcatc gaggccggag
      361 ggcttgagcc cggtgtacgg ggctccctcc ccggcacggt ggagacggac gacggtctcg
      421 aagaaccatt cgtcggcgat ctcccaggcc gcctgcccgc ccttcctggc ctggatcgcc
      481 cgttcgaagg ccctcctcac ctcgccgtcg gcctcctcgg ggacccagat gagaacgagg
      541 ttcacgtttc ccgtttcgag ggccttcttc gccgccgtga cgaccggccc gtccatggta
      601 tcgcaatggg gtggcatgtc ctttcacgtc cgaccatatt cagacagacg ctctgtcggc
      661 tacccggtat gttaagctat cgttggcttc tcgcgagtgt ttcgggcggg cgtcggtcca
      721 cgtggggacc gccatggtcc ggaaagaggt gatgacggga cgatattttc ggaaaaatat
      781 caggtcgccg ggacggggac cacgaggaac atggcgtacc catccccgat attcctcgtc
      841 acgtgacccc tgcccgaggt atcttccagg aggacgagat ccccgggccc gaaccgcctg
      901 accgtcccgt ctgtcgtctc cacctccacc gcacctgaca tgagggccag gaactgccgg
      961 gtgggagccg ggtgcagttc gccgatccag ccgggctcaa aagaatagaa gcgatacttc
     1021 gacgcgggtt tgtcctcgga gacgtaaaac ggggcagcag gtggcgctgc ttgagcgaga
     1081 ctctgtttga ccgtcacgac atcgaggtgc gagcccccca gcgcgtcggc gtatatcctg
     1141 tgatacgtca cctgggcggg tttgggtgag gatgttttcg tcatgtctgg ttctccccgg
     1201 tgagaccccg gcccttcggg gcgattcata cctcttttgc gatcttgtag agcatctcgg
     1261 cggggatggt ctccagggtc tctgtctgga cggtcccgta cttcccgaga atcgagagga
     1321 acttcaccac gctctccttg gagggcatat ccatcatcgc cacgaagtcg tacctgccga
     1381 aggtgtaatg cagggagatg agttttccgc cgagggattc gatgagattt ttcgctttcc
     1441 tgtcccgttc ctcgaggttg tctatttttt caaaggcatc gcggctcagg ttcccgaggg
     1501 tgatatagat ggtcatgtgt gaaccttgac gcattcggat gataaaaatg ttgaggtgcg
     1561 atggtgagag atggggccgg ggatcgattc tacccctcgt tatgcggatt cacgccggaa
     1621 aatgcgattc cggtcacttt ttatactgac ggtcatacaa tgctcatcaa atacctggag
     1681 agggcaggga ttttccatga aaggtaaagg gtactttatc gtcgtattat gtttcgtcct
     1741 ggctgttgcg ttcacatgcg gttgttccac gcaggggccc tcggcaggaa ccggggtcag
     1801 cccgacgccc accgggccgg cgacggatgc cggccttcgg atcgtcacgg aggacttccc
     1861 ccccttcaac tatgccggtc ccgacgggaa agcagccggc ctgtcgacag acgtggtaaa
     1921 cgccatcctc gcccggctga accagaaggc cgccatcgag gtcatgccct ggaccggggg
     1981 ctatcagctc gccctttccg gcccaagggt agttttgttt tctaccggac ggacggatga
     2041 gcgggaaacc ctcttcaagt gggtcggccc catcgcggcg aatgactata cgctgtacgc
     2101 ccggaagggc tcagccctgc agattggtag cctcgaggct gcgaagaagg ccgggctgat
     2161 aggcgtggtg aaggatgacg cacggtacca gttcctgctc gaaaaccggt tcggcaatat
     2221 cctcacctgc gatgatgaaa ccgggtgcat ccgcaacctg acggcgggaa ctaccggcct
     2281 cctcctcggg agcagcctga gtacgcccga tatcgcccgg aaagcgggga tcgatccctc
     2341 ctcgctggat gcgctctaca cgatacggac ggtcccgctc tacattgcct tcagcaggga
     2401 cacgccggac agcgtgatcg cggcctggca gggcgccctc gacgcgatga agcgggacgg
     2461 atccttcgat gccatcctcg cgaagtacgg gatgacaccg tcgggaatca cggtatccca
     2521 gtcgacctcg gaggcacagg ccggccttgc cctggagatc gtcgcagctg aaacagacgg
     2581 gcagctgaag gccgtcctcc ggccgcttga ggtcctcgcg gcaaccaccg aggcgcaatc
     2641 cggcgggtgg cagaacatca ggccgctcct cgcggttctc gaggaaaagg agccggcgat
     2701 cctcgcgtgg tacgccctcc cggacggctc gtattacacc gtcgacgacg gcctgacgag
     2761 cgccaacctg aagaaccggt cctacttccc ggtcgtcctt gccggcaacg aatccgtggg
     2821 gatcgtggtg gtgagcaagg caaccggcag gaatgtcgcc gtggtggcag tcccggtgaa
     2881 ggccgggggc ggggtgacgg gggtactggg gagctcggtg tacctcgaca ccctgaccga
     2941 tacgctgagg gaggaaatac cggcgccctt cgtcttctat gccatcgata ccgaacacaa
     3001 gttcgccctc cactcggaaa aggggcagat aaccaggaac atcgcgacca tcgggccggg
     3061 gtcctccttc ggtcaggcga tcagcacgat acttgcaggg gattccggca ccgttgagta
     3121 cgatgacggg ggcattcact acacagccag gttcagctcg gacccgctga cgggctggcg
     3181 gcttgttgtc gcatggccga aggccggtag cacaacgact acaacctgac cctttccatt
     3241 tttcccgggt cggtcggcgg ggatatcgag gaaatgaggc ggggtgcctg atccgatgaa
     3301 aggatcgggt aggttcagac accgttaagg ctgcacccgt
//
LOCUS       DATGDI010000078         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_268756, whole genome shotgun sequence.
ACCESSION   DATGDI010000078 DATGDI010000000
VERSION     DATGDI010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268756"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VKO45_02755"
     CDS             <1..1195
                     /locus_tag="VKO45_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HMA04826.1"
                     /translation="GEFRVLIATSVGEEGLDVPSTDLVIFYEAVPSEIRSIQRKGRTG
                     RSGAGKIVVLVTKGTSDEVYRYVSQTRERAMLTGMRTLQGRGGPVSPAGRSGDAGPEV
                     PKGTPQPAGSAGQPATGPEGIQRPVGPDGIQRPGDRIRTAEPSTTTPAPQPRGQTAID
                     AFAPKEPVGPEGIRGPAGVPEGIRGPGPLITADDRETSSRVVEFLSEAGARLELRRLE
                     QGDYLVGDRILVERKTARDFVDTLVERDLFGQIRELASASTRPVLVIEGGDIYTARNV
                     NPAAIRGALAAIAVDLGVAIFLTRDERETADMILTLARREEGDRGERKLHPYKSYRSL
                     KEQQEFILASFPSVGLRNARLLLAHFGSVKAVLDADEAALMAVKGIGEKTARQIFEIS
                     RQPYR"
     gene            complement(1186..1587)
                     /locus_tag="VKO45_02760"
     CDS             complement(1186..1587)
                     /locus_tag="VKO45_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sjogren's syndrome/scleroderma autoantigen 1
                     family protein"
                     /protein_id="HMA04827.1"
                     /translation="MEKKKPDDIMAECLLRGGKMLSKACRACGSPLFEVKGETLCVVC
                     RETGKEAGKVKTPRAVSKGAAAGVAGGRVPGTTGTHAPAGETGADPVAELRQTVAELA
                     RKARVEKDPKKVAALLEAAKRGAEALALLQR"
BASE COUNT          251 a          543 c          571 g          253 t
ORIGIN      
        1 gggagagttc cgggtgctca tcgcgacctc ggtgggggag gaggggctcg acgtgccctc
       61 caccgacctg gtcatcttct acgaggccgt cccgtcggag atccggtcca tccagaggaa
      121 ggggagaacc ggccggagcg gcgcagggaa gatcgtcgtg ctcgtcacaa aggggacatc
      181 tgacgaggtg taccggtatg tgagccagac gagggagcgg gcgatgctca cggggatgag
      241 gaccctgcag ggacggggcg gtcccgtctc ccccgcggga cgatccgggg atgccgggcc
      301 cgaggtcccg aaggggacac ctcaaccggc tggttcagcg gggcaaccag cgacggggcc
      361 ggaagggatt cagcggccgg ttgggccgga cgggatccag cggccggggg accggataag
      421 aaccgcagag ccatctacaa ccacacccgc gccccagccc cggggccaga ccgccatcga
      481 tgcttttgcc ccgaaggaac cggtgggtcc ggaggggatc cgggggccgg cgggggtccc
      541 tgaggggatc cgggggccgg ggccgcttat taccgccgat gaccgggaga cttcgtcccg
      601 ggtcgtggag ttcctctcgg aggcgggggc ccggctcgag ctccgccggc tggaacaggg
      661 ggactacctc gtcggcgacc ggatcctcgt ggagcggaag accgcgaggg acttcgtcga
      721 taccctggtg gagcgggacc tcttcgggca gatccgggag ctcgcctcag catcaactcg
      781 cccggtcctt gtcatcgagg gcggggacat ctatacggcg aggaacgtga accccgccgc
      841 catccggggc gccctcgccg ccatcgcggt ggacctcggg gtcgccatct tcctcacccg
      901 ggacgagcgg gagaccgccg atatgatcct cacccttgca cgccgggagg agggggaccg
      961 gggggagcgg aagctccatc cctacaagtc ctaccggtcg ctgaaggagc agcaggagtt
     1021 catcctcgcc tccttcccgt cggtcggcct gcggaacgcc cggcttctcc ttgcccactt
     1081 cggctcggtg aaggcggtcc tcgacgccga cgaggccgcg ctcatggcgg tgaaggggat
     1141 cggggagaag accgcccggc agatcttcga gatctcgcgg cagccctacc gctgaaggag
     1201 ggcgagggcc tcggcccccc gtttcgccgc ctcgaggagg gcggcaacct tcttcgggtc
     1261 cttctccacc cgggccttcc gggcgagctc ggcgacggtc tgcctgagct cggcaacggg
     1321 atcggcccct gtttctcccg ccggagcatg agtacccgtg gtcccgggaa cccttccccc
     1381 ggccaccccg gcggccgccc ctttcgacac cgcccggggg gtcttcacct tcccggcctc
     1441 ctttcccgtt tcccggcaga cgacgcagag ggtctctccc ttcacctcga agagggggga
     1501 gccgcaggcg cggcaggcct tggagagcat ctttcccccg cggaggaggc actcggccat
     1561 gatatcgtcg ggtttcttct tctccatccc gtcactccgt ttatcaaatt atatatgt
//
LOCUS       DATGDI010000079        23097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_123361, whole genome shotgun sequence.
ACCESSION   DATGDI010000079 DATGDI010000000
VERSION     DATGDI010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 23097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23097
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123361"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VKO45_02765"
     CDS             <1..963
                     /locus_tag="VKO45_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HMA04828.1"
                     /translation="GPARRGGPPGAGPAEEEGESQYLDLPGIKTILLKDIFTDIILFL
                     YTDQAAIEPAFKFAIEEGLKRGGRNFFAYHQSTIQKNFLTDIQKGTLVTKRIRKGELY
                     MLKNLLQRCTDSLSREKGPALQILLDLQDSAEFDDIVALKSLILAKKGEGLPVSGIIG
                     LNIGQMDHTLIRTFSEGIPRIIVSTGRDTMLSFAHPSFPADSVTVVPQATLEEVVKKT
                     LEPVVLSLLSRPISGYDIVREIHDRYKVLIPQARIYTLLYDLERDGYLEMKASGKSKL
                     YYPTEKGRKHIRQKLSEFKFVFQHILDGGMGEGPEKPGRGRGAP"
     gene            1435..1713
                     /locus_tag="VKO45_02770"
     CDS             1435..1713
                     /locus_tag="VKO45_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04829.1"
                     /translation="MKGEKKSQPGRVPETKFPARISFEGERARVTVDLLGIGEEKVRI
                     DLDESLVISATEGRRRFRTALDLPWEARLGSKSFRKGILELVVERAGG"
     gene            2025..3818
                     /locus_tag="VKO45_02775"
     CDS             2025..3818
                     /locus_tag="VKO45_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMA04830.1"
                     /translation="MHEVELLEAGPEGETRPGEGGGVRPRSFHVRAGREEVDSRVRQI
                     LWDHPSGLTVEEVSRAIGLGRSSTGRRLDALASRGEIRLHTYGHTRVFTVPREAGLES
                     ILSSSQPLLLVLSAGLRILGVNDPLLATFRLRREDLQGRPLKSTPLARYGEDLVASLG
                     RSARERAAGEAEMECLIGDLHHVFRTRIMPLSLAAGRRGFVVTLEDVTRRAIHRRRLE
                     GIMDEGTRGLLSSHPGLLEEILDRKRQEEQMQIVQKSVDQAPVPAFWVGRDGHFLRAN
                     RAAARMLGYGEEELARLAYPDVDAGEPPVAWDSLWETLKAGTSTDFESTFRAKRGDLL
                     RVNVRASHLVHRGREYAFLIVEDITDRKRVEDATRQANERLNLLTTVTRHDVLNDIAA
                     LGMYLELPGTGSAREFDPGTTGRLQLLVRSMGRKMEFTRDYADLGMKMPCWEDVAGCV
                     EQAAATLVPGTFRVDLDLPPLEVYADPLFERAIANLVDNTLRHGEHATRIGVRARVEG
                     DGCTLIIEDDGAGVPPGMKEPIFRPGYGRHTGLGLFLVREILGITGMSIRETGEPGKG
                     ARFEIRIPPGGFRIRDGSGDTHGDTRIQAQG"
     gene            3787..4209
                     /locus_tag="VKO45_02780"
     CDS             3787..4209
                     /locus_tag="VKO45_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04831.1"
                     /translation="METLAYRHRVEVPARVPAGGRAGAQGREEDEAVFHLTSIDNVVD
                     GRVRNPAMVKHGVIDAKRIEDLSWWIDPATHLNLDFPDHLLSEVFVASRLENGARILG
                     DANRFMYAPVDQVAYRHLGKVEPVERRRRISLRAGVPG"
     gene            complement(4215..8084)
                     /locus_tag="VKO45_02785"
     CDS             complement(4215..8084)
                     /locus_tag="VKO45_02785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II large subunit"
                     /protein_id="HMA04832.1"
                     /translation="MDLSPSMADYLGSLDRSLGGAMEVARAARSKGVDPRLSVEVPVT
                     NDLADRVEALLGIRGVAARIRELEGKMEREMLALSISGDFIARRFGETGREEVLEHAI
                     RTAMAILTEGVVAAPTEGIVRVDLGKNDSGTEYARIFYSGPIRSAGGTAQALSVLVAD
                     SVRRALNIDRYRPRPEEVERYIEEIRQYHARQHLQYFPSEKEIRLIVENCPVCIDGEG
                     TEDEEVSGYRNLERVGTNSIRGGMALVIAEGVALKAPKLEKHVRNLKLDGWEWLSGLT
                     AAKAGEKEENGIAPRDKYLRDIIAGRPVFSHPMEKGGFRLRYGRSRNTGLAAAGLSPA
                     TMHILGGFLAVGTQMKLERPGKAAGIVPVDILEGPTVRLANGDVLRIDDEKTALALAG
                     QVERILDVGEILIGFGEFLENNHPLMPAGYQEEWWRMESGAEPPASEMAALEMALGGG
                     YLHPAYSFPWEDLSPSQVATLAGRIGGTGSVRDGVLELPVDPEAKALLEELLVPHRVR
                     DKRIRVEHPLVLLACLGLTMGLERRPAWDTAPEAGSGLDLARHLSGLRMRPRAGTRIG
                     GRMGRPGKSRPREMSPPPHSLFPLGDAGGSRRSFQEARSSRGVVKKAGGGFSQTEGVI
                     EILAGERVCPSCRKTTFRNLCDCGKPTDPVFRCPRCGREAPERCPHCGIATTSGQRVP
                     LDLRAEYTAALGRLGIRENQVALVKGVKGLISRERAVEPVEKGILRSIHDLFVFKDGT
                     IRYDMIDLPLTHFRPAEAGVSVERLRELGYTRDIHGEELREPGQVLELRPQDILVSED
                     CARYLVKVAGFLDELLEKVYHLPPFYRVEEPAELVGHLLVGLAPHTSAGVLARLVGFT
                     RANVGYAHPFFHAAKRRNCFHGDTGIEVYDGERWRKYRIRDFVTENFDLTRPGLDRLG
                     TYHSEARQIAYVRAVDTAGNIHLRRILSVSVHRAPEALIRFETDRGRSIAVTPDHAML
                     VSDLAYLRKIRAMELAEGDSLPVCAGGAVITERIARREIVPSPDERVYCLTVDQDHTL
                     EANGIFTGQCDGDEDCVMLLLDCLINFSRAFLPESRGGTMDAPLMITGRIDPSEIDKE
                     SHNIDVCARYPIELYLAAERRAHPREVEGLIDRVGKRLHTPAQFEGFSYTHETSDISA
                     GPLESTYTTLETMQEKLDAMLTLAKMSRAVHPDDVAERVLSTHFIPDLQGNLRAFSNQ
                     TVRCTKCNSKYRRVPLAGKCPRCGGAIQPTVHEASVKKYLEISRRLCEEYRISPYTRQ
                     RIEVLARAIESTFGIEAEKQRALADFM"
     gene            complement(8109..8639)
                     /locus_tag="VKO45_02790"
     CDS             complement(8109..8639)
                     /locus_tag="VKO45_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMA04833.1"
                     /translation="MSGTGEEIAGRIAGLKKDDLAARHEAEEALAKMGEVAAGPLIQA
                     LRDETDPDFRWYAARVLARIGPSIIGLLIQALREEKDPQVRRYLAASLGEMGEPAIRP
                     LIGLLSDPEPALRGFAALALCRAGRPAVGPLSEALDDSDETVRSCAALILWQMGEEGG
                     MEALGKAFAPDEKETH"
     gene            complement(8636..9463)
                     /locus_tag="VKO45_02795"
     CDS             complement(8636..9463)
                     /locus_tag="VKO45_02795"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HMA04834.1"
                     /translation="MSLGTRIIGTLTLWDLIIIALTIIVALVIAQLVSLNLKKVFSGK
                     VHGRDLEVIIKIVYWAVVVAAVLIIFSQLRIDLSGLLIAGGFASIVIGIASQSVVGNL
                     ISGIFLLVERPISIGDEVLIGSLGGYVEDIRILSTLIRTHEGVYTRVPNQEVFTSNIT
                     NYVAHPARRVEYTIGVGYREDLGKVREIILGVLSASPYSLVRPEPEVSVSAIADDRLI
                     LTVYFWTPSPAFTPAKSAVLEEIQRELVGRGVEIPPPRRHVSLAPGESLPGSREGGA"
     gene            complement(9460..10191)
                     /locus_tag="VKO45_02800"
     CDS             complement(9460..10191)
                     /locus_tag="VKO45_02800"
                     /inference="COORDINATES: protein motif:HMM:NF016166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF432 domain-containing protein"
                     /protein_id="HMA04835.1"
                     /translation="MFGEFTPPYRFETDGLSLSLEMKEGHYQYMRDLAGEQSEKAISV
                     GLSRLLVHPVEPFNTGPGAVTHLEIEFPSLILEAEATETFFLTFPIEVGVFVEGIRDT
                     EVLDVFSVDQPKFSLYGSTHRGAITRFARSERHREAPSVDPTREGILKLALRNTLPEW
                     VRVSRIVLEEAQIHLYVGESAAMMAMMRIAGPNMAEITGVDRPLKGGMNRAHDLFTTR
                     RLLRLGSSRLPGMERKGFLMEEGYS"
     gene            complement(10278..11453)
                     /locus_tag="VKO45_02805"
     CDS             complement(10278..11453)
                     /locus_tag="VKO45_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HMA04836.1"
                     /translation="MELTREEEGILEGREGGTRQRMMELLAGLGKVFSASRLIPVASA
                     QVSGASYKTIGRWGLAWLESLDARVTVPTVLNPVGMDREKWREAGIPADFAENQEKVL
                     AVYRRLGVRLECTCTPYEILPTRFGEHLAWAESSAVAFANSVIGARTNREGGPGALAA
                     AIIGKTPCYGLHLDRERAPRLVIRVEGFNGAGPRPDHYGALGFLAGKIAGNRVPFLRG
                     IRPSPDELKALGAAMAASGAVALFHVEGVTPEADRWAPETGGLEEYTVTSAETGELLR
                     ETGVEAVAVGCPHCSSAELAGIARLLRGKKVRKPLFVFTSRRVMEENRGLVGEIEDSG
                     ARVYADTCMVVSPMMDPFASVMVDSGKAFAYVPSLCGALVRLGTQEECIREATRDGG"
     gene            complement(11458..12696)
                     /locus_tag="VKO45_02810"
     CDS             complement(11458..12696)
                     /locus_tag="VKO45_02810"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HMA04837.1"
                     /translation="MREFLSEMEEKGLVEEIPGPVSAEYEAAKIAAGDDRILRFRDLD
                     GKEAVMNLTANRRTLAHALGMPEEEMVPRLAGACFAGEVKIGGKLRMGEPDLASLPVM
                     KFYPGDAGRYITAGIVFARFDGVENASIHRMLVLDANRVAARLVEGRHTHSMLMAALD
                     RGERLPVAVAIGVHPAVTFASTTRVPPGMELPFAAELRGGTLPVSRCGNGVLVPEAEI
                     ILEGSIGAERAREGPFVDITGTYDEVRQEPVIEFTGMHARKDAIYASILPGGDEHKML
                     MGVPYEPIIYRAVSAVTKVRNVHLTRGGCGYLHAVIQIRKTTQGDGKNAILAAFAAHT
                     SLKHVVIVDEDIDPFDMTDVEYAVATRVRADQDLLVITGARGSSLDPRQAEDGTNVKV
                     GVDATMVLGEEARFRRAGWS"
     gene            complement(12689..13243)
                     /locus_tag="VKO45_02815"
     CDS             complement(12689..13243)
                     /locus_tag="VKO45_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HMA04838.1"
                     /translation="MGKVVVVGITGASGILYGRRIVEEIAKSAEVRLIVSETAREIGR
                     HEGVDLDGLRAMYERNDDLAALVSSGSFRHDGMVIAPCSMKTLAGVAHGYAETLVNRA
                     ADVTLKERRRCILLLREMPLSRIHLANMLAAHDAGATVMVASPAFYHRPQTIGQLVDM
                     VVARVLDHLEIPHDLPVRWRGYDA"
     gene            complement(13263..14468)
                     /locus_tag="VKO45_02820"
     CDS             complement(13263..14468)
                     /locus_tag="VKO45_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMA04839.1"
                     /translation="MKFIKDPVHGYVEVEAWLLPVLDSPPVQRLRHVKQLGFSCLVYP
                     GANHTRFEHSLGTMHLATLLARHLALDPAEVRLIAAAGLLHDIGHGPFSHTSEAFLSE
                     FAGHDHHRVDHLLAEPALAEALAEADLDPAAISALIRGEHPLADLIHGDLDADRMDYL
                     LRDAHYTGVPYGTVDAERLIQSSILLDGGLGIEESGVQAAESLLIARTLMRPAVYFHH
                     TSRIATSMLTLALCEHTSGGSADPRAIPLLDDGGLCQALAGSESAVARSLLDGLMGRR
                     LYKRALYVGRDQVGGTLLTDRPSVKRMRALAREIATAAGREDWEVIVDVPPFPVDMSM
                     EVQVRNRSATLDLSLVSPLVNTLNEARRSQWRLGVYAPREHVAEVEQAAVGVLHVKKA
                     TRQNTLASG"
     gene            14519..15427
                     /gene="cofD"
                     /locus_tag="VKO45_02825"
     CDS             14519..15427
                     /gene="cofD"
                     /locus_tag="VKO45_02825"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HMA04840.1"
                     /translation="MITFLSGGTGTPKLLQGMRELLPDREMAVVVNTAEDVWISGNHL
                     SPDFDTVMYLFAGLADTATWWGLSGDTFVTHDAIAAWGGEEALKIGDRDRAIHILRGE
                     MLRKGMRLTEAAALLAKRLGVEAALLPMSDTPVATLLETDHGLLHFQDYWVKRRGMPR
                     VREVVRRFTEPPRASPEVLQAIGKADGVVIGPSNPVTSIAPILECAGVPEALGEKFVV
                     AVSPFLGDRPVSGPAAELMRAWGREPSSQGTFDLYRDLLDVFVQDIRDPVKIGGAVRL
                     DTLMKDRPASEALAREILAMVRGRGS"
     gene            15480..16217
                     /locus_tag="VKO45_02830"
     CDS             15480..16217
                     /locus_tag="VKO45_02830"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMA04841.1"
                     /translation="MTPSAVGAMGARGDVEGLIRALAPGLPAEVRRAAAGILGDLVDR
                     RAVDPLIEILGDREEQVRLAAVLSLAKIADPKAVPALLALLADADGDVRTATVISLGM
                     IGDRSAVPALLTALSDRHTPVRDAVAWALGAMKDERAVRHLIRALSDPEWHVRMEAEW
                     ALLEIGQPAVDLLISALHDEGVRWKAAGVLVKIGAVSLEPLQRALQGTADPNIRQGGE
                     WAVRKIADLKTKMNERRPAPRRGPPGM"
     gene            16225..17184
                     /locus_tag="VKO45_02835"
     CDS             16225..17184
                     /locus_tag="VKO45_02835"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMA04842.1"
                     /translation="MEIFDVFRPNIERLRERGDREGLIRALGYPRDAAVRRAAVKALG
                     DAPDPALLDTLAKALGDPDGGVRYEAVEALVRLKDRRAVEPLSERLLDTDGDVRFAAA
                     WALGEMGDPEAVEALVAALRDENWRVRTEALHALSRLRDVEAMEAIRGMLSDGYWRVR
                     VEAAHALGHLGDPGAVPSLAGALGDENWRVRREVVKALAAIGPPETLEPLVRAIGDTD
                     ENVRREAASALGALGTRAIGPLSGILRGEAREGRREAARTLGKIRDPAAIEVLISILQ
                     DSDPQVRDEALLALGRMGGSAVKPLLKAMRERGFPDVLETEEP"
     gene            complement(17181..18518)
                     /locus_tag="VKO45_02840"
     CDS             complement(17181..18518)
                     /locus_tag="VKO45_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HMA04843.1"
                     /translation="MSRAIPRVVIGGTWSGCGKTTVARGLMAAFAARGLAVQPFKVGP
                     DFIDPTHHTAICGRPSRNLDPFMMGEEEVARVFARASEGADLAIIEGVMGLFDGMDGG
                     DVSSTAQVAELLSAPVVLVVDPRGMSGSVHAVIRGYSTYRPGASPAGIIFNRVASPRH
                     RALIGTGLSARAMGWIPAREELSIGSRHLGLVMAPEVERPGRIEAGIFEECCNLDDIL
                     ELARAAPPLPVPRALSATQPERVRIGVARDAAFCFTYQENLDRLARAGADLEFTSPLT
                     DPFPDVDGLYLPGGYPELHARALSGSPFLRGLRTACGDGMPVYGECGGLLVLSRSLSA
                     GEGEVAMAGVLPARATITGKVQALGYVEARATSTASLLPPGLSFRGHEFHYSAIECER
                     DARFSLDLGRGKGIRGGRDGLTEGNTVGTYTHAYFTDEFAESLVRAAEKSRRA"
     gene            complement(18515..19690)
                     /locus_tag="VKO45_02845"
     CDS             complement(18515..19690)
                     /locus_tag="VKO45_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA04844.1"
                     /translation="MSGIILEARDVRYRYPHGMEAIRGISFHVRRGEKVALVGPNGAG
                     KSTLLLMFNGMLRPDSGMLLFDNRPLVYDRESLRELRRRVGFVFQNPDRQFIAPTVWQ
                     DVAFGPTNLGLSPGQVGEAVESALRNVGLAGLGDRPPHQLSSGEKKRVAIAGVLAMDP
                     DLLVLDEPTSNLDPAGSEDIMELLEEQRGNGKTVFISTHDVELAYPWADRVILLRRGE
                     ILREDSPEVAFGDPDLVRRARLSVPVLLDLHRELGKRGLPLPGKRPRTVLDLVHTIER
                     AARTGGVRPAPGTIHVCNVDETGKETLSRWLSAHGGVSLGAMGTRAKQVASEAGLDLD
                     FSYGVIDKCMLASLNGRSSLILTTGGMVQQVGRRVSAFREESGFEIPVEKVGEVKGG"
     gene            complement(19692..20489)
                     /gene="cbiQ"
                     /locus_tag="VKO45_02850"
     CDS             complement(19692..20489)
                     /gene="cbiQ"
                     /locus_tag="VKO45_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HMA04845.1"
                     /translation="MYEEFLEDIAQGNGLREVSPSLKLGAALGAILLCLLSPGFPGPL
                     AIAALLSLGILLLARVDPRTYGRLLLAPFSFALVSAGAILLLTGAGGAFFSWTPLPWL
                     SLSITPAGINEGILVLSRMTGGMSALIFLAVTTPMTDLFMVMRRYGVPEPVLDLAMMI
                     YRSIFTILDQLVQTYQAQRMRLGYSSFRESLRSFSNLAGSVFIAGWIAGEDLVRAMDA
                     RCYDGRFAVPGGSRLVETRHVLWVVAFLLVSSAMVVFTRGMTVMPGG"
     gene            complement(20508..20801)
                     /locus_tag="VKO45_02855"
     CDS             complement(20508..20801)
                     /locus_tag="VKO45_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HMA04846.1"
                     /translation="MLRRHRFGILVMAALMFLGILALWAGYRGYPGSDDRGSAGVAGL
                     TGTPEEQVRPLVWQWVPQGAGTEAALFALQAGIGVAIIGWALWRWRGTGEREE"
     gene            complement(20801..21493)
                     /locus_tag="VKO45_02860"
     CDS             complement(20801..21493)
                     /locus_tag="VKO45_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HMA04847.1"
                     /translation="MHIMEGFLPVSWCLLWWLAALPCLALGIRELRRVLSRDREALPL
                     LGMTGGFVFILSSLKLPSVTGSSSHPTGTGLPAIAFGPFITTVIGTIVLLFQALFLAH
                     GGLSTLGANIISMGVAGPVAGWGVYRLLRNTRANMYATVFLASACADLFTYVTTSLQL
                     ALAYPAAVGGVGSSLAVFLAIFALTQIPLAILESVVLVLVFRYIVQLRPGILVRLKLM
                     PEDRIAAPAGGA"
     gene            complement(21538..22122)
                     /locus_tag="VKO45_02865"
     CDS             complement(21538..22122)
                     /locus_tag="VKO45_02865"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HMA04848.1"
                     /translation="MKIVGVGCGPGLLTEEAILALRGARRVYGSRRAIEIARHALPRS
                     CEIHEIRDYSSLEFPEDAILLSTGDPMLAGLGNREGEVVPGISSLQLAFARLRLPLAQ
                     VSVVDGHGRDPGQAIGEAVEELRRGKFVFLIPDPAFPTSDLAAAIGREDIPCTIALCE
                     NLGYPEERIILGTPDRPPAGGSRLFSLVIGRIPL"
     gene            complement(22119..>23097)
                     /locus_tag="VKO45_02870"
     CDS             complement(22119..>23097)
                     /locus_tag="VKO45_02870"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HMA04849.1"
                     /translation="PDAWVRACRAPALLPLVEEGLALLTADGTVLRRGFTTGTTAAAA
                     CKAAILSLGGDEVEEVSVTLPCGIEVDLPARAREGEGSSVKDAGDHPSDVTGGLEFVA
                     SARPSEDELVLEFGEGIGRYARDVPGHAIGSPAVSGPALSSITGASLEGLEGAGCHGA
                     VVSLRVPRGREVASRTLNPRLGIEGGISLLGTTGLVEPWDEHLVSSVLERVRAAETPV
                     LTTGRTGLMYSRLLFPAREVILVGGRIGEALEASRGQAILCGLPGLILREICPGILEG
                     TGCDTVADLLARQGWREVVEPCLSAFHRRMPGVRVVLIDRNGLVLGGVP"
BASE COUNT         3842 a         7637 c         8050 g         3568 t
ORIGIN      
        1 ggaccggccc ggaggggggg gcctcccggg gcgggacctg cggaggagga gggagaatcc
       61 cagtacctgg acctccccgg catcaagacc atcctcctca aggacatctt caccgacatc
      121 atcctgttcc tgtacaccga ccaggcggcc atcgagcctg ccttcaagtt cgccatcgag
      181 gaggggctga agaggggggg gcggaacttc ttcgcgtacc accagtccac catccagaag
      241 aacttcctca ccgacatcca gaagggaacc ctcgtgacga agcggatcag gaagggggaa
      301 ctgtacatgc tgaagaacct cctccagcgg tgcaccgact ccctctcaag ggagaagggg
      361 cccgccctcc agatcctcct cgacctgcag gactccgccg agttcgacga catcgtcgcc
      421 ctgaaaagcc tcattctcgc gaagaagggg gagggcctgc ccgtgtcggg gatcatcggc
      481 ctcaacatcg gccagatgga tcatacgctg atccggacct tctcagaggg aattccccgg
      541 atcatcgtct cgaccgggag ggacacgatg ctctccttcg cccacccctc cttccccgcg
      601 gattcggtga cggttgtccc ccaggcgacc ctcgaggaag tggtcaaaaa gaccctggag
      661 cccgtggtcc tctccctcct ctcccggccc atttccgggt acgatatcgt gagggagatc
      721 catgaccggt acaaggtgct catcccccag gcgaggatct acaccctcct ctacgatctc
      781 gagagggacg ggtacctcga gatgaaggcc tcggggaagt ccaagctcta ctaccccacc
      841 gagaagggga ggaagcatat ccggcagaag ctctcggagt tcaagttcgt cttccagcat
      901 atcctggacg gcggaatggg ggaggggccg gaaaagccgg gacggggccg cggtgcgccc
      961 tgaacagatc ttccgatcct ttacttttca gaatttgaaa caaagggtga gagttcggag
     1021 aacgtatatc tggtgccata ggtgacgggg tggtgtgggt gtaacagccc acacagcagt
     1081 ggaagacacc gtggagagac tgatctctgt catggcctgc cgtggagcat cggtggtgcc
     1141 atggcgggga tcacatggga cggacggtcg tatccgggag aatccaggaa cgcctgtccg
     1201 gaggagacaa acggtggaga gcacatttcc acatgccaga aggatcgtgt tcacaaggaa
     1261 gaaaatggtc accccagagg cgcgaatctg ccagccagct cagtatataa ccccgaatct
     1321 ctctctttta ggtgattcca gcctctgggg gcctgcattc cgcccctggt cccggcagag
     1381 acccgccggg ggaccgttga aaggttcctc tccggccgag gggggaacct tcccatgaaa
     1441 ggtgaaaaga agtctcaacc cggccgggtt cccgaaacaa aattccccgc ccgtatctcc
     1501 tttgaagggg aacgggcccg cgtcaccgtg gatctcctgg ggatcggcga ggagaaggtc
     1561 cggatcgatc tcgacgagag cctggtcatc tcggcgaccg aggggaggcg gcggttccgg
     1621 actgccctcg accttccctg ggaggcgagg ctcggttcga agagcttcag gaaagggatc
     1681 ctggaactcg tcgtggagag ggcgggcggc tgaggcgata ggatctgatg agccggaggc
     1741 cgtgcccagg gagaccatgt tcatccgtgg gagcctggga gggcagattt ccggattgac
     1801 gtggtccaac gggactatca tcccccggga ccgtggtctt ccagaccccc tgtcctttca
     1861 gaaccatggt cttccagatc ctggtctttt caaggactga agtcctttca gaaccgtggt
     1921 cttccagacc ccggtctttt caaattctgt cacaaaattt gatcctcggg atagtatcta
     1981 taaaactcgg cgcgagatcc gtctctctgg cacgggagga tcagatgcac gaggtggaac
     2041 tgctggaggc cgggccggag ggcgaaaccc gtcccgggga aggtggagga gtcaggccca
     2101 gatcctttca tgtgcgggcc gggagggagg aggtggattc ccgggtcagg cagatcctct
     2161 gggatcaccc gagcggcctg accgtggagg aggtgtcccg ggccatcggg ctcggccgga
     2221 gctcgaccgg acggcggctg gatgccctcg cctcgcgggg ggagatccgg ctccacacct
     2281 atgggcacac ccgggtattc acggtccccc gggaagcagg cctggaatcc atcctttcct
     2341 catcccagcc ccttctcctg gtcctctcgg ccggcctcag gattctcggg gtgaacgatc
     2401 ctctccttgc cacattccgg ctccggaggg aggatctcca gggcaggccg ctgaaaagca
     2461 ccccccttgc ccggtacggg gaggacctcg tcgcctccct cgggagatcg gcccgggaaa
     2521 gggcggccgg ggaggccgag atggagtgcc tcatcgggga tctccatcac gtcttcagga
     2581 cgaggatcat gcccctctcc ctggcagcgg ggcggagagg gttcgtggtc accctggagg
     2641 atgtcacccg gagggccatt caccggcgga ggctcgaggg aatcatggac gaggggacac
     2701 ggggtctcct ctcctcccat ccgggtctcc tcgaggagat ccttgaccgg aagcgacagg
     2761 aggagcagat gcagatcgtc cagaagtccg tggaccaggc tcccgtcccc gctttctggg
     2821 tcggcaggga cgggcatttc ctccgggcga accgggcggc ggcccggatg cttggttacg
     2881 gggaggagga actggcccgg ctcgcctacc cggacgtgga cgccggcgaa cccccggttg
     2941 catgggattc cctctgggag accctgaagg cgggaacgtc cacggatttc gagagcacct
     3001 tccgggcgaa acggggggat ctgctccggg tgaacgtgag agccagtcat cttgtccacc
     3061 ggggcaggga gtacgccttc ctcatcgtgg aggacatcac cgaccgaaag cgggtcgagg
     3121 atgccacgcg gcaggcgaac gagaggctga acctcctgac caccgtgacc cggcatgatg
     3181 tcctgaacga catcgccgcc ctcggcatgt acctcgagct tcccgggacg ggctctgccc
     3241 gggagttcga ccccgggacg accggaaggc tgcagctcct cgtccgatcc atggggcgca
     3301 agatggagtt cacccgggac tacgcagacc tcgggatgaa aatgccctgc tgggaggatg
     3361 tcgccgggtg cgtggagcag gcagccgcta ctctcgtccc gggcactttc agggtcgacc
     3421 tcgacctccc gcccctcgag gtctatgcag accctctctt cgagcgggcc atcgcaaacc
     3481 tggtggacaa cactctcagg cacggggagc atgccacccg catcggggtc cgggcgcggg
     3541 tggaggggga cggctgcacc ctcatcatcg aggacgacgg ggcgggagtt ccccccggga
     3601 tgaaggaacc catcttcagg ccggggtacg ggaggcacac gggcctcggt ctcttcctgg
     3661 taagggagat cctcggcatc accgggatga gcatccggga gaccggtgag ccgggaaaag
     3721 gtgcaagatt tgagatacgg atcccccccg gcgggttccg tatcagggac ggatcaggtg
     3781 atacacatgg agacactcgc atacaggcac agggttgagg tacctgcccg ggtccccgcg
     3841 gggggaaggg cgggcgccca gggaagggaa gaggatgaag cagttttcca tctcacgtcc
     3901 attgacaacg tcgtggacgg gagggtgcgg aatccggcca tggtgaagca cggcgtcatc
     3961 gacgccaaga ggatcgagga tctctcatgg tggatcgatc ccgccacgca cctgaacctc
     4021 gactttccgg accatctgct gagcgaggtg tttgtggcat ccaggctgga gaacggtgcc
     4081 aggatcctcg gggacgcgaa caggttcatg tacgcccccg ttgaccaggt ggcataccgg
     4141 catctcggga aggtagagcc ggtggagagg cggcggcgga tcagcctgag ggcgggagtc
     4201 cccgggtagc ctgatcacat aaagtcagcg agggcccgct gcttctcggc ctcgatcccg
     4261 aaggtggact cgatggcccg ggcgaggacc tcgatccgct gcctggtgta cggggagatg
     4321 cggtactcct cgcagagcct cctcgagatc tccaggtatt tttttaccga ggcctcgtga
     4381 accgtcggct ggatggcccc gccgcacctc gggcatttcc cggcgagggg cacccgccgg
     4441 tacttggagt tgcacttcgt gcaccgcacc gtctggttcg agaaggcccg gaggttgccc
     4501 tgcaggtcgg ggatgaagtg ggtggagagg acccgctcgg ccacatcgtc ggggtggacc
     4561 gcccgggaca tcttcgcgag ggtgagcatg gcatcgagct tctcctgcat ggtctcgagg
     4621 gtggtgtagg tggactcaag ggggcccgcc gagatgtcgc tcgtctcgtg ggtgtaggag
     4681 aacccctcga actgggcggg ggtgtggagc ctttttccca ccctgtcgat gaggccctcc
     4741 acctccctcg ggtgggcgcg ccgctccgcg gcaaggtaca gctcgatggg gtaccgggcg
     4801 cagacgtcaa tgttatggga ctccttgtcg atctccgagg ggtcgatacg cccggtgatc
     4861 atgagggggg cgtccatggt cccgccccgg gactcgggga ggaaggcccg ggagaagttg
     4921 atgaggcaat ccagaagaag catcacgcag tcctcatccc cgtcgcactg gccggtgaag
     4981 atgccgtttg cctccagggt gtggtcctgg tcgacggtga ggcagtagac ccgctcgtcc
     5041 ggcgagggca cgatctccct ccgggcgatc cgctcggtga tcaccgcgcc cccggcacag
     5101 accgggagcg agtctccctc cgcgagctcc atcgccctga tcttccggag ataggcgagg
     5161 tccgagacga gcatggcatg gtcgggcgtg acggcgatgg acctgccccg gtcggtctcg
     5221 aaccggatga gggcctcggg agcccggtgg acggagaccg agaggatccg ccggaggtgg
     5281 atgttccctg ccgtgtccac ggcccggaca taggcgatct gccttgcctc cgagtggtag
     5341 gtcccgagcc ggtccaggcc ggggcgggtg aggtcgaagt tctcggtgac gaagtcccgg
     5401 atccggtact tcctccaccg ctccccgtcg tagacctcga tcccggtgtc cccgtggaag
     5461 cagttccgcc gcttcgccgc gtggaagaag gggtgggcat acccgacgtt cgcccgggtg
     5521 aacccgacga gccgggcgag gaccccggcc gaggtgtggg gggcgaggcc gaccaggagg
     5581 tgccccacga gctccgcggg ctcctccacc cggtagaagg gcgggaggtg gtagaccttc
     5641 tccaggagct cgtcgaggaa gccggccacc ttgacgaggt accgggcgca gtcctcggag
     5701 acgaggatgt cctggggccg gagctcgagt acctggcccg gctcccggag ttcctccccg
     5761 tggatgtccc gggtgtagcc cagttcccgg aggcgctcca cgctcacccc ggcctccgcg
     5821 ggacggaagt gggtgagggg gaggtcgatc atgtcgtacc ggatggtccc gtccttgaag
     5881 acgaagaggt cgtggatgga gcggagaatc cccttctcca ccggctccac cgcccgctcc
     5941 cgggagatga gccccttcac ccccttcacg agggcgacct ggttctcccg gatgcccagc
     6001 cggccgaggg cggcggtgta ctcggcccgg aggtccaggg ggacccgctg ccccgaggtc
     6061 gtggcgatgc cgcagtgggg acaccgctcg ggggcctccc tgccgcaccg ggggcagcgg
     6121 aagaccgggt cggtgggctt cccgcagtcg cagaggttcc ggaaggtcgt tttccggcag
     6181 gaggggcaca cccgctcccc ggccaggatc tcgatgaccc cctcggtctg ggagaagcct
     6241 cccccggcct tcttcaccac gcccctcgat gacctcgcct cctggaagga acgccggctc
     6301 ccgccggcat caccgagggg gaagagggag tggggcggcg ggctcatctc ccggggccgg
     6361 gacttccccg gcctccccat ccgcccgccg atccgggtcc cggcccgggg gcgcatgcgg
     6421 aggccggaga ggtgacgggc caggtcgagg ccggagccgg cctccggcgc ggtgtcccag
     6481 gccggccgcc tctcgaggcc catggtgagg ccgaggcagg cgaggaggac gagagggtgt
     6541 tccacccgga tccgtttgtc ccggacccgg tgggggacca ggagctcctc gaggagggcc
     6601 ttggcctccg ggtcgacggg gagctcgagc accccgtcac ggacagaccc cgttcccccg
     6661 atcctccctg cgagggtggc cacctgggag ggggagagat cctcccaggg aaacgagtag
     6721 gccgggtgga ggtaccctcc cccgagggcc atctcgaggg cggccatctc gctcgcaggg
     6781 ggctcggccc cgctctccat cctccaccac tcctcctggt agccggccgg catgaggggg
     6841 tggttgttct ccaggaactc cccgaagccg atgaggatct cccccacgtc caggatccgc
     6901 tccacctggc cggcgagggc aagcgccgtc ttctcgtcgt cgatccggag cacgtcgccg
     6961 tttgcgagcc ggaccgtggg gccctcgaga atgtcgacgg ggacgatccc cgcggccttc
     7021 cccggccgct cgagcttcat ctgggtcccc acggcgagga accccccgag gatgtgcatg
     7081 gtggcggggg agagcccggc ggcggcgagg ccggtgttcc gggacctgcc gtaccggagc
     7141 cggaacccgc ccttctccat ggggtgggaa aagaccggcc ggccggcgat gatgtcccgg
     7201 aggtacttgt cccggggggc gatcccgttc tcctccttct cgccggcctt tgcggcggtc
     7261 aggcccgaga gccactccca tccgtcgagc ttcaggttcc ggacatgctt ctcgagcttc
     7321 ggggccttga gggcgacccc ctcggcgatg acgagggcca tcccgccccg gatggagttt
     7381 gtcccgaccc gttcgaggtt cctgtacccg ctcacctcct cgtcctcggt cccctccccg
     7441 tcgatgcaga ccgggcagtt ctcgacgatg agccggatct ccttctcgct cgggaagtac
     7501 tggaggtgct gccgggcgtg gtactggcgg atctcctcga tataccgctc cacctcctca
     7561 ggccggggcc ggtaccggtc gatgttcagg gcccggcgga cagagtccgc cacgaggacc
     7621 gagagggcct gggccgtccc cccggcgctc cggatggggc cggagtagaa gatccgggcg
     7681 tactcggtcc cggagtcgtt cttcccgagg tcgacccgga cgatcccctc ggtgggggcg
     7741 gccacgaccc cctcggtgag gatggccatc gcggtgcgga tggcatgctc gaggacctcc
     7801 tccctgcccg tctccccgaa ccgccgggcg atgaagtcac ccgagatgga gagggcgagc
     7861 atctcccgct ccattttccc ctcgagctcc cggatccggg ccgccacccc ccggatcccg
     7921 aggagcgcct ccacccggtc cgcgaggtcg ttcgtcacgg ggacttcgac ggagagacgg
     7981 gggtccaccc ccttcgaccg ggccgcccgg gcgacctcca tcgcaccccc gagggagcga
     8041 tcaagggagc cgagatagtc ggccatggaa ggggacaggt ccatacccta gcatgggacg
     8101 gggggtatct aatgggtttc tttttcgtcg ggggcgaagg ccttcccgag ggcctccatc
     8161 cccccctcct cccccatctg ccagaggatg agggcggcgc aggaccggac cgtctcgtcc
     8221 gaatcgtcca gggcctcgga gaggggcccg acggccggcc ttcccgcccg gcagagggcg
     8281 agggccgcga agccccggag cgcgggctcg gggtcagaga ggagtccgat gaggggccgg
     8341 atggccggct cgcccatctc cccgaggctc gccgccaggt agcgccggac ctgggggtcc
     8401 ttctcctccc ggagcgcctg gatcaggagc ccgatgatgg aaggaccgat ccgggcgagc
     8461 acccgggccg cgtaccaccg gaaatcgggg tccgtttcat cccggagggc ctggatcagg
     8521 ggcccggccg cgacctcgcc catcttcgcg agggcctcct cggcctcgtg ccgggcggcg
     8581 agatcgtcct tcttcaggcc ggcgatccgg cccgcgatct cctctcccgt cccgctcacg
     8641 cccctccctc tctggagccc ggcaggctct ccccgggcgc gagggagacg tgacgcctgg
     8701 gcgggggtat ctccacgcct ctccccacga gttcgcgctg gatctcctcg aggaccgcgg
     8761 acttcgcggg cgtgaaggcg ggggaggggg tccagaagta cacggtgagg atgagccggt
     8821 cgtcggcgat ggccgagacc gagacctccg gctctggccg gacgagggaa tagggggagg
     8881 cggaaaggac cccgaggatg atctcgcgca ccttccccag gtcctcccgg tagcccaccc
     8941 cgatggtgta ctccacccgc cgcgccgggt gggcgacgta gttcgtgatg ttgctcgtga
     9001 agacctcctg gttcgggacc ctcgtgtaga ccccctcgtg ggtgcggatg agggtggaga
     9061 ggatacggat gtcctccacg taccccccca ggctcccgat gaggacctcg tccccgatgg
     9121 agatggggcg ctccacgagg aggaagatcc ccgagatcag gttcccgacg accgactggc
     9181 tggcgatccc gatgacgatg ctcgcaaagc ccccggcgat gaggaggccc gagaggtcga
     9241 tccggagctg cgagaagatg atgaggacgg cagccacgac cacggcccag tacacgatct
     9301 tgatgataac ctcgaggtcc ctgccatgga cctttcccga gaagaccttc ttcaggttca
     9361 gggagacaag ctgggcgatg acgagggcga cgatgatggt gagggcgatg atgatgaggt
     9421 cccagagggt gagggtgccg atgatgcggg tcccgaggct cacgagtacc cctcctccat
     9481 gaggaagccc ttcctctcca tccccgggag ccgcgaggag ccgagccgga ggagcctcct
     9541 cgtggtgaag aggtcatggg cccggttcat gccccccttc aggggccggt ccaccccggt
     9601 gatctcggcc atgttgggtc ccgcgatccg catcatcgcc atcatggcgg cggactctcc
     9661 gacgtagaga tggatctggg cctcttcgag gacgatgcgg gagacccgga cccattcagg
     9721 gagggtgttc cgcaaggcga gcttgaggat gccctcccgc gtgggatcga cggaaggagc
     9781 ctcccggtgc ctctcgctcc gggcgaaacg ggtgatggct ccccggtggg tggagccgta
     9841 taaggagaac ttcggctggt cgaccgagaa gacatcgagg acttcggtgt cccggatccc
     9901 ctccacgaag acccccacct cgatagggaa ggtgaggaag aaggtctccg tggcctcggc
     9961 ctcgaggatg agggagggaa actcgatctc caggtgggtc acggcccccg ggccggtgtt
    10021 gaagggctcc acgggatgga cgaggaggcg ggagaggccg acggagatgg ccttctcgga
    10081 ctgttcgccg gcgaggtccc gcatgtactg gtagtgcccc tccttcatct cgagggagag
    10141 ggaaagaccg tccgtttcga accggtacgg gggggtgaac tcaccgaaca caggagagct
    10201 tcagaggaga aagacaaata ccttccccca aggggtaaaa tcctgccgtg accgtcgggt
    10261 cttaccgggg gaccgggtta tcccccgtcc ctcgtagcct cccggatgca ctcctcctgg
    10321 gtcccgaggc ggaccaaggc cccgcacagg gaggggacgt aggcgaaggc ctttccgctg
    10381 tcgaccatga ccgaggcgaa gggatccatc atcggggaaa ccaccatgca ggtgtcggca
    10441 tagacccggg cacccgaatc ctcaatctct ccgaccaggc cccggttctc ctccatcacc
    10501 cggcgggagg tgaagacaaa gaggggcttt ctcacctttt tcccccggag cagccgggcg
    10561 atgccggcga gctcggcgga ggagcagtgg gggcatccca ccgccaccgc ctccacgccg
    10621 gtttcccgga ggagctcacc ggtttccgcg gaggtcacgg tgtactcctc gagaccgccg
    10681 gtttccgggg cccaccggtc cgcctccggg gttacccctt cgacgtggaa gagggcgacc
    10741 gccccgctcg ccgccatggc ggcccccagg gccttcaact cgtcgggaga gggccggatc
    10801 ccccggagga agggaacccg gtttccggcg atctttccgg cgaggaaccc gagggccccg
    10861 tagtgatccg gccggggacc ggccccgttg aatccctcca cccggatgac gagccggggg
    10921 gcccgctccc ggtcgaggtg aagcccgtag cagggggtct tcccgatgat ggccgcggcg
    10981 agggccccgg gccctccctc ccggttcgtc cgggccccga tgaccgagtt cgcgaacgcc
    11041 accgccgagg actcggccca ggcgaggtgc tcgccgaacc gggtcgggag gatctcgtac
    11101 ggggtacagg tgcattccag gcgtacaccg agacgccggt agaccgcgag gaccttctcc
    11161 tggttctccg cgaagtcagc gggaattccc gcctcccgcc atttctcccg gtccatcccc
    11221 accgggttca ggacggtggg gacggtcacc ctcgcgtcca ggctctccag ccatgcgagc
    11281 ccccacctcc cgatggtctt gtacgaggct ccgctcacct gggcggaggc gacggggatg
    11341 agccgcgatg cggagaagac cttgccgagc ccggccagga gctccatcat cctctgccgg
    11401 gtcccgccct cccggccctc gaggatcccc tcctcctccc gggtgagctc catctcccta
    11461 ggaccacccc gcccgccgga accgcgcctc ctccccgagg accatggtcg cgtccacccc
    11521 caccttcacg ttcgtcccgt cttcagcctg ccggggatcc agggaggagc cccgggcgcc
    11581 ggtgatgacg aggaggtcct ggtcggcccg cacccgggtg gcgacggcat actccacgtc
    11641 agtcatgtcg aaggggtcga tatcctcgtc cacgatcacc acgtgtttca gggaggtgtg
    11701 ggcggcgaag gctgcgagga tggcgttctt gccatccccc tgggtggtct tccggatctg
    11761 gatgacggcg tggaggtacc cgcacccgcc ccgggtgagg tggacgtttc gtaccttcgt
    11821 caccgccgaa accgcccggt agatgatggg ctcgtagggt acccccatga gcatcttgtg
    11881 ctcgtccccg ccgggaagaa tgctcgcgta gatggcatcc ttcctggcat gcatccccgt
    11941 gaactcgatg acgggttcct ggcgcacctc gtcgtaggtc ccggtgatgt ccacgaaggg
    12001 cccctcccgg gcacgctcgg ccccgatgga accctcgagg atgatctcgg cctcggggac
    12061 gaggacaccg tttccgcacc gcgacaccgg aagggttccc cccctgagct cggccgcgaa
    12121 ggggagctcc atcccggggg gaacccgggt cgtcgaggcg aaggtgaccg ccgggtggac
    12181 cccgatggcg accgcaacgg ggagcctctc gccccggtcg agggccgcca tgagcatgga
    12241 gtgggtgtgc ctcccctcca cgagccgggc tgcgacccgg ttcgcgtcga ggacgagcat
    12301 ccggtggatg gaggcgttct ccaccccgtc gaaccgtgcg aagacgatcc ccgcggtgat
    12361 gtaccggccg gcatccccgg ggtaaaactt catgaccggg agggaggcga gatcgggctc
    12421 tcccatcctg agttttcccc cgatcttcac ctctcccgca aagcatgcgc cggccaggcg
    12481 cgggaccatc tcctcctcgg gcatcccgag ggcatgggcg agggtcctcc ggtttgccgt
    12541 gaggttcatc accgcctcct tcccgtcgag gtcccggaac cggaggatcc ggtcgtcccc
    12601 ggcggcgatc ttcgcggcct cgtactccgc agagaccggg cccgggatct cctcgacgag
    12661 gcccttctcc tccatctccg agagaaattc acgcatcgta acccctccag cggaccggga
    12721 gatcgtgggg gatctccagg tggtcgagga ccctcgcgac gaccatgtcg acgagctgcc
    12781 cgatggtctg cggccggtgg tagaaggcgg gggaggccac catgacggtg gcccccgcat
    12841 catgggcggc gagcatgttc gcgagatgga tccgggagag gggcatctcc cggaggagga
    12901 ggatgcacct ccggcgctcc ttcagggtga catcggccgc ccggttcacc agggtctcgg
    12961 cgtacccgtg ggcgaccccg gcgagggtct tcatgctgca gggggcgatg accatgccgt
    13021 cgtgccggaa ggacccgctc gagacgaggg cagcgaggtc atcgttcctt tcgtacatcg
    13081 cccggagccc gtccaggtcc actccctcgt gccggccgat ctcccgggcg gtctcagaga
    13141 cgatgagccg tacctcggcc gatttcgcga tctcttccac gatcctcctc ccatagagga
    13201 tcccgcttgc ccccgtgata ccgacaacca caacctttcc catgtgcacc ttcccccgta
    13261 ttttacccgc ttgccagggt gttctgcctc gtagccttct tcacgtggag caccccgacc
    13321 gccgcctgct ccacctccgc cacgtgctcc cgcggcgcgt acaccccgag ccgccactgg
    13381 cttcgcctcg cctcattcag ggtgttcacc aggggagata cgagggagag gtcgagggtg
    13441 gccgaacggt tcctcacctg tacctccatg gacatgtcaa ccgggaaggg tgggacgtcc
    13501 acgataacct cccagtcctc cctccctgcc gcggtcgcga tctcccgggc gagggcccgc
    13561 atccttttca cggagggcct gtccgtaagg agtgtgcccc ccacctggtc cctgcccacg
    13621 taaagcgccc tcttgtaaag cctcctcccc atgagcccgt cgaggaggga tcgggcgacg
    13681 gcgctctccg agccggcgag ggcctggcag agccccccgt cgtccaggag cgggatggcc
    13741 cgggggtctg cggatccgcc cgaggtgtgt tcgcagaggg cgagggtaag catcgaggtt
    13801 gcgatccggc tcgtgtggtg gaaatacacc gccggccgca tgagggtccg ggcgataagg
    13861 agggactcgg ccgcctggac cccgctctcc tcgatcccga ggccgccatc caggaggatg
    13921 ctcgactgga tgagcctttc ggcatccacc gtgccgtacg ggacaccggt gtagtgggcg
    13981 tcccggagga ggtagtccat ccggtcggcg tccaggtccc cgtggatcag gtcagcgagg
    14041 gggtgctcgc cccggatgag ggccgagatg gccgccgggt cgagatccgc ctccgcgagg
    14101 gcctccgcga gggccggctc ggcgaggagg tggtcgaccc ggtggtggtc gtgccccgcg
    14161 aactccgaga ggaaggcctc gctcgtgtgg gagaagggcc catgcccgat gtcatggagg
    14221 agcccggcgg ccgcgatgag ccggacctcg gcggggtcca gggccaggtg ccgggcgagg
    14281 agcgtcgcga ggtgcatggt tccgagggag tgctcgaacc gggtgtggtt cgcccccggg
    14341 tagaccaggc aggagaaccc gagctgcttc acgtgccgga ggcgctggac cggcggggaa
    14401 tcgaggacgg ggaggagcca tgcctcgacc tccacgtagc cgtgcaccgg gtccttgatg
    14461 aacttcatcc gataccgttt aagaatcatc aaatattcat tgataatagt attgggtgat
    14521 gatcaccttc ctctccgggg ggaccgggac accgaaactc ctccagggca tgcgggagct
    14581 ccttccggac cgggagatgg cggtggtggt gaacaccgcc gaggatgtct ggatctcggg
    14641 gaaccacctc tccccggatt tcgacacggt gatgtacctc tttgccggcc tcgccgatac
    14701 cgcgacatgg tggggcctct ccggcgacac cttcgtgacc cacgacgcca tcgcggcgtg
    14761 gggaggggag gaggccctga agatcggcga ccgggaccgg gccatccaca ttctccgggg
    14821 agagatgctc aggaagggga tgcgcctcac cgaggccgcg gccctcctcg cgaaacgcct
    14881 cggggtggag gcggccctcc tccccatgtc cgacaccccc gtcgcgaccc ttctcgaaac
    14941 cgaccacggc ctcctccact tccaggacta ctgggtgaag cgccggggaa tgccccgggt
    15001 gagggaggtg gtgaggaggt ttaccgagcc tcccagggcg agccccgagg tgctccaggc
    15061 catcggaaag gccgacgggg tggtcatcgg cccctcgaac cccgtcacct ccatcgcccc
    15121 catcctggag tgcgccggtg ttccggaggc cctcggggag aagttcgtgg tggccgtgag
    15181 ccccttcctc ggggacaggc cggtgagcgg cccggcggcg gagctcatga gggcctgggg
    15241 gagggagccc tcctcccagg ggaccttcga cctgtaccgg gacctcctcg acgttttcgt
    15301 ccaggacatc cgggaccccg tgaagattgg gggggcggtc cggctcgaca ccctcatgaa
    15361 ggacaggccg gcgagcgagg ccctcgcccg ggagattctc gcgatggtcc gggggagagg
    15421 atcatgaagg ggcatcccct accggtgaac gggggtcggg agcagtgacc ggccaggaga
    15481 tgacgccatc ggccgtcggg gccatggggg cccgcgggga cgtcgaggga ctcatccggg
    15541 ccctcgcccc cggcctcccg gcggaggtgc ggagggccgc cgccgggatc ctcggggatc
    15601 tcgtggaccg gagggccgtg gaccccctca tcgagatcct cggcgaccgg gaggagcagg
    15661 tccggctcgc ggcggtcctc tccctcgcaa agatcgcgga tccgaaggcg gtccccgccc
    15721 tcctcgccct cctcgcggac gcggacgggg acgtccggac cgcgaccgtc atctccctcg
    15781 gcatgatcgg ggaccggtcc gcggtccctg ccctgctcac cgccctctcg gaccgtcaca
    15841 cgccggtcag ggacgcggtg gcctgggccc tcggggccat gaaggacgaa cgggcggtga
    15901 ggcacctcat ccgggccctc tccgaccccg agtggcacgt gcggatggag gccgagtggg
    15961 ccctcctcga gatcgggcag ccggccgtcg acctcctcat ctcggcgctt catgacgagg
    16021 gtgtcaggtg gaaggcggcg ggggtgctcg tgaagatcgg ggccgtttcc ctggaacccc
    16081 tccagagggc cctccagggg accgccgacc cgaacatccg gcagggcggg gaatgggcgg
    16141 tgaggaagat agcggatctg aagacgaaga tgaatgaacg caggccggct ccccgccggg
    16201 gtcccccggg gatgtgagga agggatggag atcttcgacg tcttccggcc gaacatcgag
    16261 aggctccggg aaagggggga ccgggaggga ctgatccgtg ccctcgggta cccgagggat
    16321 gccgcggtcc ggagggcggc ggtgaaggcc ctcggcgatg ccccggaccc ggccctcctc
    16381 gacaccctcg caaaggccct cggggatccc gacgggggag tgaggtacga ggcggtggag
    16441 gctcttgtgc ggctgaagga ccggagggcg gtggagcctc tctcagagag gctcctcgat
    16501 accgacggcg atgtgcgctt cgccgccgcc tgggccctcg gggagatggg ggacccggag
    16561 gcggtggagg ccctcgtcgc ggccctcagg gacgagaact ggagggtccg gaccgaggcc
    16621 ctccatgccc tctcgaggct ccgcgatgtg gaggcgatgg aggccatccg ggggatgctc
    16681 tcggacggct actggcgggt gagggtcgag gcggcccacg ccctcggcca tctcggggat
    16741 ccgggcgccg tcccgtctct cgccggggct ctcggggacg agaactggag ggtcaggcgc
    16801 gaggtggtga aggccctcgc ggccatcggc ccccccgaga ccctggagcc cctggtccgg
    16861 gccatcggcg ataccgacga gaacgtccgg cgggaggcgg cctcggccct cggcgccctg
    16921 gggacgaggg ccatcgggcc cctctcggga atcctccggg gggaggcccg ggagggccgg
    16981 cgggaggcgg cgaggaccct cgggaagatc cgggaccctg ctgccatcga ggtcctcatc
    17041 tccatcctcc aggactcgga cccgcaggtg agggacgagg ccctcctcgc cctcgggagg
    17101 atggggggat cggcggtcaa gcctctcctg aaggcgatgc gggagagagg ctttcccgat
    17161 gtcctggaaa cggaagagcc ctaagccctc cgggattttt cggcggcccg gaccagggat
    17221 tcggcgaact cgtccgtgaa gtacgcgtga gtatacgtac ccacggtgtt cccctcggtg
    17281 agtccgtccc ggccgccgcg gatgcccttt ccccggccga ggtcgaggga gaaacgggca
    17341 tcccgttcgc attcgatggc cgagtagtgg aactcgtgtc cccggaagga gaggcccggg
    17401 gggaggagtg aggcggtgct cgttgcccgg gcctcgacat agccgagggc ttggaccttc
    17461 cccgtgatgg tcgcccgggc ggggaggacc cccgccatgg cgacctcgcc ctcccccgcc
    17521 gagagggacc gggagaggac gaggaggccc ccgcattcgc cgtagacggg catgccgtct
    17581 ccgcaggcgg tgcggagccc gcggaggaag ggggatcccg agagagccct ggcgtggagc
    17641 tcggggtacc cccctggcag gtagagcccg tcaacgtcgg ggaagggatc ggtgaggggg
    17701 ctcgtgaact cgaggtctgc acccgcccgg gcgagccggt cgaggttctc ctggtaggtg
    17761 aagcagaagg ccgcgtccct ggccacgccg atccgcaccc tttccggctg tgtcgccgaa
    17821 agcgcgcgtg gcacgggaag agggggagcc gctctcgcaa gctcgaggat atcgtcgaga
    17881 ttgcagcact cctcgaagat gcccgcctcg atccggccgg gcctctccac ctccggtgcc
    17941 atgacgagcc cgaggtgcct gctcccgatg gagagctcct cccgggcggg gatccacccc
    18001 atggcccggg ccgaaaggcc tgtcccgatg agggcccggt gcctcgggct cgccacccgg
    18061 ttgaagatga tgccggccgg cgatgcgccg ggccggtacg tcgagtatcc ccggatcacc
    18121 gcgtggaccg aaccagacat gccccgcgga tccacgacga ggacgacggg agccgagagg
    18181 agctccgcca cttgggcggt gctcgagaca tcgcccccgt ccattccgtc gaagagcccc
    18241 atcactccct cgatgatggc gaggtcggcg ccctccgagg cccgggcaaa gacccgggcc
    18301 acctcctcct cccccatcat gaagggatcg aggttcctcg aggggcggcc gcagatggcc
    18361 gtgtggtggg tgggatcgat gaagtcaggg cccaccttga agggctggac cgcgagtccc
    18421 cgggcggcga aggccgccat gagcccccgg gcgacggtgg tcttgccgca gccgctccag
    18481 gtgccgccga tgacgaccct cgggatggcc cggctcatcc ccccttcacc tcccccacct
    18541 tctccaccgg tatctcgaag ccggactcct cccggaaggc cgagacccgt cgccccacct
    18601 gctgcaccat tcccccggtc gtgaggatga gggaggacct cccgttcagg gacgcgagca
    18661 tgcacttgtc gatgaccccg tacgagaagt cgaggtcgag gcccgcctcc gaggcgacct
    18721 gcttggcccg cgtccccatc gccccgaggg agacaccgcc gtgggcggaa agccaccggg
    18781 agagcgtctc ttttccggtc tcatccacat tgcagacatg gatggttccc ggggcagggc
    18841 ggactccccc ggtccttgcc gctctctcga tggtgtgtac caggtcgagg acggttctcg
    18901 gccttttccc cgggagaggc aggccacgtt tcccgagctc ccggtggaga tcgaggagga
    18961 ccgggacaga gagtcgtgcc ctgcggacga ggtccgggtc cccgaaggcc acctccgggg
    19021 agtcctcacg caggatctcc cctctgcgga ggaggatcac ccggtcggcc cagggatagg
    19081 cgagctctac gtcgtgggtg gagatgaaca cggtcttccc gttcccccgc tgctcctcca
    19141 ggagctccat gatgtcctcg gagccggccg ggtccaggtt gctcgtcggc tcatcgagga
    19201 cgaggaggtc ggggtccatg gcgaggaccc cggcgatggc cacccgcttc ttctcaccgc
    19261 tcgagagctg gtgcgggggc cggtccccca ggccggcgag cccgacgttc cggagggccg
    19321 actccaccgc ctctccgacc tgccccgggg agaggccgag gttcgtcggg ccgaaggcga
    19381 cgtcctgcca gaccgtcggg gcaatgaact ggcggtccgg gttctggaag acgaacccga
    19441 ccctccgccg gagttcccgg agggactccc ggtcgtacac gaggggccgg ttgtcgaaga
    19501 ggagcatccc cgagtcgggc cggagcatgc cgttaaacat gaggaggagg gttgacttcc
    19561 ctgcaccgtt cgggccgacg agggcgacct tctcgccccg gcgcacgtgg aagctgatcc
    19621 cccggatcgc ctccatgccg tgcgggtacc ggtacctgac atcccgtgcc tcgaggatga
    19681 tgccgctcat ctcatccccc cggcatgacc gtcatccccc gcgtgaaaac gaccatggcc
    19741 gatgacacga ggagaaatgc caccacccag aggacgtgcc gggtctccac cagccgggat
    19801 cccccgggca ctgcgaacct cccgtcgtag caccgggcat ccatggcccg gacgaggtcc
    19861 tcgccggcga tccagcccgc gatgaagacc gacccggcca ggttcgagaa ggaccggagg
    19921 gattcgcgga aggacgagta gccgagccgc atccgctggg cctggtacgt ctggacgagc
    19981 tggtcgagga tggtgaagat ggatcggtag atcatcatcg cgagatcgag gaccggctcc
    20041 ggcaccccgt accgcctcat gaccatgaaa agatcggtca tgggggtggt gaccgcgagg
    20101 aagatcaggg cggacatgcc cccggtcatc cgggagagga cgaggatccc ttcgttgatg
    20161 cccgccggcg tgatggagag ggagagccac ggaagggggg tccagctgaa gaaggcaccc
    20221 ccggctcccg tgaggagaag gatcgcgccc gcgctcacga gggcgaagga gaagggtgcg
    20281 aggagaagcc tcccgtacgt ccggggatcg acccgggcga ggagcagaat cccgagggag
    20341 aggagggccg cgatggcgag gggcccggga aagcccgggg agaggagaca gaggagaatg
    20401 gcccccaggg ccgctcccag cttcagggag gggctcacct cccgaaggcc gttcccctgg
    20461 gcgatgtcct cgaggaactc ctcatacatc gtcgcggccc tcccggttca ttcctcccgt
    20521 tcccccgtgc ccctccatcg ccagagggcc cagccgatga tagctacccc gatgcccgcc
    20581 tgcagggcga agagggcggc ctccgtcccg gcgccctgcg gcacccattg ccacacgagg
    20641 gggcggacct gttcttcggg tgtaccggtc agcccggcca ctccggccga cccccgatcg
    20701 tcggagccgg gatacccccg gtatcccgcc cagagcgcga ggattccgag gaacatgagt
    20761 gcagccatta caaggatccc gaaccggtgt ctccggagca tcacgcaccc ccggcaggtg
    20821 cagcgatccg gtcttccggc atgagtttca ggcggacaag gatccccggc cggagctgga
    20881 cgatgtaccg gaagacgagc acgaggacca ccgattcgag gatggcgagg ggaatctggg
    20941 tcagggcaaa tatggccagg aacacggcca gggagcttcc gaccccccct accgcggcgg
    21001 ggtaggcaag ggcgagctgg agggaggttg tcacataggt gaacaggtcg gcacaggccg
    21061 aggcgaggaa caccgtcgcg tacatgtttg cccgggtgtt cctgaggagc cggtacaccc
    21121 cccagccggc cacggggccg gcaaccccca tggagatgat gttcgcccca agggtggaaa
    21181 gccccccgtg agcgaggaag agggcctgga agagaaggac gatggtcccg atgaccgtgg
    21241 tgatgaaggg cccgaaggcg atggcaggga ggcccgtccc cgtcggatgg gatgaactcc
    21301 ccgtgaccga ggggagtttc agggaggaga ggatgaagac gaagccgccg gtcatcccga
    21361 ggaggggcag ggcctcgcgg tcccgtgaga ggaccctgcg cagctcccgg atccccaggg
    21421 caaggcaggg gagggcggcg agccaccaga ggaggcacca cgagacgggg aggaatccct
    21481 ccatgatatg cacgggaaca ccacacaatt ttacttgttt taactacata gaacttgtta
    21541 taagggaatc cggccgatga ccagggaaaa gagccgggat ccacccgcag ggggccggtc
    21601 gggggtcccc aggatgatcc gctcctccgg gtatcccagg ttctcgcaga gggcgatggt
    21661 acaggggata tcctctctcc cgatggcggc ggcaagatcg ctggtgggga aggcgggatc
    21721 cgggatgagg aagacaaact tcccccggcg gagctcctcc acggcctccc cgatggcctg
    21781 gccggggtcc cgtccatgcc cgtcgaccac cgagacctgc gcgaggggga gccggagccg
    21841 ggcaaaggcg agctggagcg aggagatgcc gggaaccacc tccccctccc ggttcccgag
    21901 gccggcgagc atgggatcgc ccgtggaaag gaggatggcg tcctcaggga attccaggga
    21961 tgagtagtcc cggatctcgt ggatctcgca ggaacgggga agggcatgcc tcgcgatctc
    22021 gatggcccgc cgcgagccgt acacccggcg ggctccccgc agggcgagga tcgcctcctc
    22081 ggtgagcagg ccgggcccgc acccgacccc gacgatcttc acggcacgcc ccccaggacg
    22141 agcccgttcc tgtcgatgag gacgaccctg accccgggca tcctgcggtg gaaggcggaa
    22201 aggcagggct ccaccacctc cctccacccc tggcgggcca ggagatccgc gacggtatca
    22261 cagcccgtcc cctcgaggat gccggggcag atctcccgga ggatgagccc ggggagcccg
    22321 cagaggatcg cctggccccg ggaggcctcc agcgcctccc ctattcttcc cccgaccagg
    22381 atcacctcac gggcggggaa gaggagccgg gagtacatga ggcccgtcct tcccgtggtg
    22441 aggaccggcg tttcggcggc ccgtacccgc tccagcaccg aggagaccag gtgctcgtcc
    22501 cagggctcca cgaggccggt ggtcccgagg agggagatgc ccccctcgat cccgagcctc
    22561 gggttaaggg tccgggacgc cacctctcgc ccccggggga cccggaggga gaccaccgcg
    22621 ccatggcagc cggccccctc cagcccctcg agggatgccc cggtgatgga ggagagggcg
    22681 ggcccgctca ccgccggtga cccgatggcg tgcccgggaa catccctcgc gtacctgccg
    22741 atcccctccc cgaactcgag aacgagttca tcctccgaag gcctcgcgga cgcgacaaac
    22801 tcgagcccgc ccgtcacgtc ggaggggtgg tccccggcgt ccttcaccga ggagccctcc
    22861 ccctcccggg cccgggcggg gaggtcgacc tcgatcccgc aggggagggt gacggagacc
    22921 tcctccacct cgtcaccgcc gagggagagg atggccgctt tgcaggccgc cgcggcggtg
    22981 gtgccggtgg tgaagccgcg ccggagcacg gttccatcgg ccgtgagaag ggcgaggccc
    23041 tcttcaacca gggggaggag ggccggggcc ctgcaggccc gtacccaggc atcgggg
//
LOCUS       DATGDI010000080         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_414306, whole genome shotgun sequence.
ACCESSION   DATGDI010000080 DATGDI010000000
VERSION     DATGDI010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414306"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VKO45_02875"
     CDS             complement(<1..798)
                     /locus_tag="VKO45_02875"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04850.1"
                     /translation="MKKTLYFLLGIALIGTLLVAGCAQQQAVPPQQTPAPTTVPLTTV
                     QTTIQTPAAPAGYTVKLGDTSLGKVLTGADGMTLYFFITDLAGEGVSTCYGSCASFWP
                     VFYASTISVEPPLRASDFSSITRTDGTMQTTYKGRPLYSFANDKRPGDVKGENVLKTW
                     YVAKTDYTVMIASRPETGAFLTDSTGRALYIWLKDTTLGDSACRGTCAATWPPFSAST
                     LVVPSALSLTDFSTFTRSDGMRQTAFMGRPLYYYSLDKDAGDLYGQGI"
     gene            915..1223
                     /locus_tag="VKO45_02880"
     CDS             915..1223
                     /locus_tag="VKO45_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HMA04851.1"
                     /translation="MLTEIVHGIAFFFGGAGALLITYGGIRAIAEILGRELLGIEKGY
                     DVIRKPFTEKIIFGLEFFIAADIINTLIAPGQQEILVLGAVVLIRVALGYFLSREARG
                     "
     gene            complement(1356..>2272)
                     /locus_tag="VKO45_02885"
     CDS             complement(1356..>2272)
                     /locus_tag="VKO45_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMA04852.1"
                     /translation="TLEASWAAVAAIVIGYLVMGIRIVRPTHRVLIEFLGKYQKYGNP
                     GLYWILPGIQKLYTVNITEKLVNADRQEIITSDNLNAFVDAQIYFKVRIDEESVKASQ
                     YNVFNYEDQIVALARTTLRNIIGGMTLKTANSERNTINLNLAEALTKETAAWGIQIVR
                     AELKEIQPPPDVQETMNKVVKALNEKTAAIDFATATETNADGQRRAAIKQAEGVKQAT
                     ILEAQGKAEAIRLVNEAAKQYFQGTAITLRQLEVAENALATNTKIIVPAGQSVVNVIG
                     GLLGVETGKQADPGTPNPVKSLWMPPGQ"
BASE COUNT          370 a          675 c          741 g          486 t
ORIGIN      
        1 gataccctgg ccgtagaggt ccccggcatc cttgtctaac gagtagtagt agaggggccg
       61 gcccatgaac gccgtctgcc gcatcccgtc cgaccgggtg aaggtgctga agtctgtgag
      121 gctcagggcc gagggaacca caagggtgct cgctgagaag ggcggccagg tggcggcaca
      181 ggtccccctg catgcactgt cccccagcgt ggtatccttc agccagatgt agagagcccg
      241 gcctgtgctg tcggtcagga aggcccctgt ctccggccgg ctcgcgatca tcaccgtgta
      301 atccgttttc gccacgtacc atgtcttcag gacattctcg cccttcacgt cgccgggcct
      361 cttgtcgttc gcgaaggaat agaggggacg gcccttgtag gtcgtctgca tcgtcccgtc
      421 ggtcctcgtg atggacgaga agtcagatgc ccggagggga ggctctaccg agatggtgct
      481 tgcgtaaaag accggccaga acgaggcaca gctcccgtaa caggtgctca ccccttcgcc
      541 cgcgaggtcg gtgatgaaga agtacagggt catcccgtct gccccggtga ggaccttccc
      601 gagggaggta tccccgagct tgacggtata cccggcagga gctgccgggg tctggatggt
      661 cgtctggacg gttgtaaggg gcaccgtggt cggtgcaggc gtctgctgcg gcggcacggc
      721 ctgctgctgc gcacagcctg cgacgaggag agttccgatc agggcgattc cgaggaggaa
      781 atacagggtc tttttcatga agatcacctt tcacgctgat ggagccagtc ccgcggcatg
      841 ctgataaata ttgggggagt ttacaaaaag agaaacttta aacacctccg ggcggtatcc
      901 actccggagg ttcgatgctc accgagatcg tccacgggat cgcttttttc ttcgggggag
      961 ccggcgccct cctcatcacc tacggcggga tccgggccat tgccgagatc ctggggcgcg
     1021 agctgctcgg gattgaaaag ggctacgatg tcatcaggaa acccttcacc gagaagatca
     1081 tcttcgggct cgagttcttc atcgccgcgg atatcatcaa taccctcatc gcgcccggcc
     1141 agcaggaaat ccttgttctc ggggccgtgg tcctcatcag ggtggccctc gggtatttcc
     1201 tttcccgcga ggctcggggc tgacgtatcc cggagggctg ttccgttccg gattgtttcc
     1261 cggcggaaca gtgagcccgg gaacacgatt cggatcagat cgatagaggg atcttccggg
     1321 ttctggcacc tggaaaaata ggtgatatcc tcctgtcact gaccgggcgg catccagagg
     1381 gatttgaccg ggttcggggt gccggggtcg gcctgtttcc cggtctccac gccgaggagg
     1441 cccccgatga cgttcaccac gctctggccg gcggggacga tgatcttcgt gttggtcgcg
     1501 agggcgttct cggcgacctc gagctgccgg agggtgatgg ccgtgccctg gaagtactgc
     1561 ttcgccgcct cgttcacgag ccggatggcc tcggccttcc cctgggcctc gaggatcgtc
     1621 gcctgtttga ccccctcggc ctgcttgatg gcggcccgcc gctggccgtc ggcgttcgtc
     1681 tcggtggccg tggcgaagtc gatggccgcg gtcttctcgt ttaaggcctt caccaccttg
     1741 ttcatggtct cctggacgtc gggaggcggc tggatctcct tgagctcggc ccggacgatc
     1801 tggatccccc aggcggctgt ctccttcgtg agggcctcgg cgaggttgag gttgatggtg
     1861 ttacgctcgg agttggcggt cttcagggtc atcccgccga tgatgttccg gagcgtcgtc
     1921 ctcgcgaggg ccacgatctg gtcctcgtag ttgaagacat tgtactgcga ggccttcacg
     1981 ctctcctcgt cgatcctgac cttgaagtag atctgggcgt cgacgaaggc gttcaggttg
     2041 tcgctcgtga tgatctcctg ccggtcggcg ttcacgagct tctcggtgat gttcacggta
     2101 tacagcttct ggatgcccgg gaggatccag tacagccccg ggttgccgta cttctggtac
     2161 ttccccagga actcgatgag cacccggtgg gtgggcctca cgatccgtat ccccatgacg
     2221 agatagccga tcacgatggc ggcgacggcc gcccaggacg cttcgagggt ga
//
LOCUS       DATGDI010000081        29655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_356028, whole genome shotgun sequence.
ACCESSION   DATGDI010000081 DATGDI010000000
VERSION     DATGDI010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 29655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29655
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356028"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VKO45_02890"
     CDS             complement(<1..410)
                     /locus_tag="VKO45_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase/dehydrogenase beta
                     subunit N-terminal domain-containing protein"
                     /protein_id="HMA04853.1"
                     /translation="MASDPYLGKYTTCVAARSTDKEILQKSQDGGIATALMVYALEQG
                     IIDGAIVAGKGDRPWQPRPMVAMSREEILKAQGTKYNISPQISWLKEATRSFGLDKVG
                     ITGVCCQMQATRKAQLYPINMRDVPGKIALAVGL"
     gene            complement(421..1182)
                     /gene="frhG"
                     /locus_tag="VKO45_02895"
     CDS             complement(421..1182)
                     /gene="frhG"
                     /locus_tag="VKO45_02895"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit gamma"
                     /protein_id="HMA04854.1"
                     /translation="MANKIKLGHVHMSGCTGCLVSVADNYLGLIKILDDYADLVYGIA
                     LADVRHIPEMDVALVEGSICIQDKESVEEIKETRKKSKIVVALGSCASYGNITRFCRG
                     GQHNQPQHESFLPIGDLIDVDVYIPGCPPSPELIRNVAVMAYLLLQGNADQKALAGKY
                     LKPLMALAKQTTKASYNNLMNDVVDQGLCIGCGICAAACPVRAITHEFGKPQGQRDLC
                     INCGSCYGSCPRSFFNFDVIPEFEGISEIIAGALK"
     gene            complement(1271..1753)
                     /gene="frhD"
                     /locus_tag="VKO45_02900"
     CDS             complement(1271..1753)
                     /gene="frhD"
                     /locus_tag="VKO45_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-reducing hydrogenase, FrhD
                     protein"
                     /protein_id="HMA04855.1"
                     /translation="MEASLYPEIVVAGCGNPLYADDGFGTAVVESLQGLELPENVKVI
                     DAGLGGPHFVFTLLDPESTKKLIIVDIADFGGEPGELRWLAVDELPVGSYRDAHSWDL
                     TEPLQRIKDDIRIRVLACQKKYVSAPEMVIGITDEVQRSIPGAVSEILKEIGMNYGTA
                     "
     gene            complement(1759..3126)
                     /gene="frhA"
                     /locus_tag="VKO45_02905"
     CDS             complement(1759..3126)
                     /gene="frhA"
                     /locus_tag="VKO45_02905"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit alpha"
                     /protein_id="HMA04856.1"
                     /translation="MSKVVEISPTTRHEGHSKLTLKVDDQGIVERGDWLSITPVRGVE
                     KLAIGKTMEQVPKIASRVCGICPIAHCLAGIESMEASVGCEIPMDAKLLRVILHAANR
                     LHSIALHNILILPDFYIPGTETKINPFTKEQPVRSVAARIIRLREIGQTIGAIAGGEA
                     VHPSNPRVGGMYRNVSPRAKQKMADLAKEGLVLAREQMDFMIAVIRNMQKREWTDVAG
                     KKIPLPKTLGFHDQGVMATSPMYGSSSLDEKPMWDFARWKETRPWDWYQGEMTVDLED
                     PTYPIGGTTKAGTKVNPQMEACNGVPTYDGQPVEVGPRARLSTFKNFPEKGTFAQHIA
                     RQLEYPDCLYSILKCLDNLDTSGKVLADNIPQGDGSMGWAANEAPRGTDVHLARVKDG
                     RVLWYEMLVPTTWNLPTCSRGLTGAPWQIAEMVVRAYDPCVSCATHMIVVDQENRLVA
                     QKLIQ"
     gene            complement(3425..4387)
                     /gene="mptA"
                     /locus_tag="VKO45_02910"
     CDS             complement(3425..4387)
                     /gene="mptA"
                     /locus_tag="VKO45_02910"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HMA04857.1"
                     /translation="MTQTSSDLPDVQASQPEVCINLTRVGVTNVKKLVEVPRPNKRPV
                     IFISTFDVFVDLPAQLKGANLSRNFEVIDEVLQQAIDGEVKEVEQLCSTVARKLLDRH
                     EYADRTEVTMRSQFMQRRETPVSMTACHEVVMVYAKAVARRNDSNPFVRKSIGAEVTG
                     MTACPCAQDIMKDRAAHVLRNLGIPQEKVDAFFTEVPMATHNQRGKGFLCIETDDDQR
                     VGLEDIIDILKDSMSARIYELLKRGDESYVVLEAHKNPRFVEDCVREIARKVLGTFAS
                     LPADSVVTIKQTNEESIHQHNAYAERKATLAELMVEMEGELSGQ"
     gene            4492..5490
                     /gene="ilvC"
                     /locus_tag="VKO45_02915"
     CDS             4492..5490
                     /gene="ilvC"
                     /locus_tag="VKO45_02915"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HMA04858.1"
                     /translation="MEPTIPRYFDDDADLGVLKGKTIAVIGYGSQGRGQSLNLRDSGL
                     SVIIGLRPGKSWDRAKKEGFEVLPVKDAVKKADVVQILVPDELQAAAYRADIGPNLKP
                     KTCLMFSHGFNIHFGQIVPPTTVDVIMVAPKGPGAMVRRTYEEGKGVPALIAIHQDAS
                     GKAKDIALAYAKGIGATRAVVLETSFREETETDLFGEQAVLCGGCTSLIKAGFETLVD
                     AGYAPEMAYLEILHELKLIVDLIHEGGLTNMRAAISNTAQYGDLTRGPRVIGPEVYGV
                     MQELLDEIQSGEFAKEWILENQVNRPVFNALTKADEEHLIEEVGKDLRSLMPQFRK"
     gene            5674..6153
                     /locus_tag="VKO45_02920"
     CDS             5674..6153
                     /locus_tag="VKO45_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HMA04859.1"
                     /translation="MSVCIVYHSETGNTKMVAEVVAKATGAVMIPVRDTANYGKISRY
                     LVGARKAMAGEKAVIEPSKIDVSAHDLVIVGTPVWAWRPTPGANAAVEALTGCEGKKG
                     MVFATCGGKPGETLGILKAALEARKVKVEGAFSFAKRDLGDAKKIGEFIDAVKKASG"
     gene            complement(6328..8397)
                     /gene="purL"
                     /locus_tag="VKO45_02925"
     CDS             complement(6328..8397)
                     /gene="purL"
                     /locus_tag="VKO45_02925"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HMA04860.1"
                     /translation="MQTEDLSLLRRELGRDLTDVEMACFENLWSEHCSYRSTKTLLRT
                     LPTEGKDVIVGPGDDAAVVRYSEDLAVVVGMESHNHPSYVDPYHGSATGVGGIVRDVV
                     SMGGRPIALLDPLYFGTFEHEKNRYLFEHVVAGIGDYGNCIGVPVVGGEMGVDPSYRG
                     NPLVNVVCVGIVHPDRYLTARAKRPGSHLLLVGSSTGRDGLGGASFASRDLSEDSEAE
                     DRPSVQIGDPYTEKLIIDATLEMAETGKVLSCKDLGAAGLAGASSEMCASFGALIHAD
                     RIHLREAGMTPPEIMLSESQERMLFEVAAADVPAMGAIAEKYDLGWSDIGEVIPECRY
                     IIRFRGKVVADLPIQLLAGDAPLCFWEKRPAPARAPFRTPVANLKNLALAVLSHPEVA
                     GKDWVTEQYDHDVQLRSISLGRDAAVLRLGQGALVLSCGCNPRQIALAPYEGTANSLL
                     ENAANLAALGATPLCIVNCLNFASPIHPEIYWQIEQCILGLGDMARRLKIPVVGGNVS
                     LYNESDEFGTKILPTPSIGLLGKGEVRRWKAAPAGSLLALVGETKPEYGGSVLDAVAG
                     CGGDPPSVADPGVINAIRELVAGDRCEAATDLSQGGLLSALAALVPSAKVVLGPDALC
                     ELFSESAGRFLLAVKEESDLKGLSYRVIGEAGGEGLRIRAGTESLTLSPEEMESARTS
                     LTRLMRS"
     gene            8606..9220
                     /locus_tag="VKO45_02930"
     CDS             8606..9220
                     /locus_tag="VKO45_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HMA04861.1"
                     /translation="MPQSYEDLLKKAYSQITEVESSHERFQVPPGKVFIEGKTTVLEN
                     LAEIADILNRSPEDLLKYLVGELGTAGKIEGNRAVFNGKFEPASVNALIRSYVEDYVI
                     CSECGRPDTRLVKDGRIPILRCDACGGHRPVKKRKVRLEPQEERPQEGTVVELSILSV
                     SRRGDGVARMGKYTIYVAGAKPGEKVKVKITKVAGSVAFTERAQ"
     gene            complement(9304..9570)
                     /locus_tag="VKO45_02935"
     CDS             complement(9304..9570)
                     /locus_tag="VKO45_02935"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit L"
                     /protein_id="HMA04862.1"
                     /translation="MEIKILELSKDRARIVFPGEGHTFMNLLTDEVLKDPAVDVARYT
                     MEFQHSDPEFFITTNRKDPIKVIRDACKRISGYCDEIMKGIKEA"
     gene            complement(9842..10564)
                     /locus_tag="VKO45_02940"
     CDS             complement(9842..10564)
                     /locus_tag="VKO45_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA04863.1"
                     /translation="MELRFLGTGDAVGTPKVGCDCETCRYAREAGIQRLRTSLLIEEG
                     GRHLLIDTSPDLRQQLLCAGSPGIDAVIWTHGHYDHYSGYGEFYRVQDPPPVYAADET
                     LSYCAPLFAFLPFEGHRVEPFLPFEFSGLTVTLFPVNHPPVPTFGVRIDSPGGSVAYT
                     GDTTSDIPERSRRLLSGVDLLILDAIVPPEIRLNKHMNYEDALRMAGEAHPGEYRLVH
                     LSHLIPWTLPHLARDGECILFT"
     gene            complement(10566..11846)
                     /locus_tag="VKO45_02945"
     CDS             complement(10566..11846)
                     /locus_tag="VKO45_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMA04864.1"
                     /translation="MAAEGTNEVMQVMEILLTAEIFNRNRDLDGNDLPPRCREAFGMG
                     PDGQVKRPVIVSDGTVAQATGIGDASEKVKGNPFVELDSFGHRMRISALDAAAGWFLK
                     KGGKAQITRNPALASFFEGFDSAGVSYRDVKAGNPRVEDTRACLDARIAALTVGDEAL
                     KAALELLIVSAPDEVEPRLSDLICTGQQAAIASKVRTALLHRDLLRSHRIYELGKLLF
                     VGPPGTGKTTLALALSRELHMPMLEVRLSMVTSQYLGETSKNIDRIFEFAKKIAPAIL
                     FIDEFDFVAKSRITDDHGAMKRAVNMLLKNIDRVSLVRDGVLLLAATNHPQLLDEAAW
                     RRFDEVVEFFLPDAGMRKAILAKVTNSLPWDGDLGELAGMTEGFSGADLRVLVKEALL
                     SSLVRESSRIEREDIERGFTLVTNRNMLRGTPSG"
     gene            complement(11891..12766)
                     /gene="map"
                     /locus_tag="VKO45_02950"
     CDS             complement(11891..12766)
                     /gene="map"
                     /locus_tag="VKO45_02950"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HMA04865.1"
                     /translation="MKDEVLDTYLEAGKIASRILSRSLPLVRAGAPFLGVVEEVEALV
                     TGEGAGLAFPLNLSLNEAAAHDTASEGDDRIFRAGDVVKMDLGVHLEGYIADTAGTVD
                     LGENAPLLRASRAALEKAIALVRPGVSTGDLGAAIQAEIEALGFRPVANLTGHGLGRY
                     EIHTPPTVPNIRVSGGAVLKEGMVFAIEPFASTGTGFVSDGPRVQIFQQVLERPARLP
                     AAKRILEQVKDRHGLPFARRWLTGERLEISLSGLVRSGQLHAYPVLRDVPGSLVSQAE
                     HTVIVTGDGCIVSTR"
     gene            complement(12763..13911)
                     /locus_tag="VKO45_02955"
     CDS             complement(12763..13911)
                     /locus_tag="VKO45_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HMA04866.1"
                     /translation="MPSTLLPMESLDDAIAAAGAKAYVAYGSSLDADMRYLTRFRTTD
                     PVLFVKKSGEPGTIIVSQMEYERAARESTAIPVTRAQAGLTEILAEEKNRWKAYARMI
                     AGIAGGDVLVPPQFPAALARELEAFSRFTVDDDTVLKQRAVKSDDEIAAIARVQEATE
                     AAMGGAISSIRSARARKGILYRGKTPLTSEILQTEVAVALLEHGCRAVDTIISCGEDS
                     AIPHIRGSGALREDAPIILDIFPQEEESGYTADMSRTVVRGDPSPEILEMFEAVRGAQ
                     SLAATLVRPGATGAEVHGKVVDYFRERGFESNTRGFIHNLGHGVGLEVHELPVLGPGG
                     GELSAGNVITLEPGLYYPGRGGVRLEDMGVVTPDGFRDLTKMPRDLRA"
     gene            13952..14200
                     /locus_tag="VKO45_02960"
     CDS             13952..14200
                     /locus_tag="VKO45_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04867.1"
                     /translation="MDEHARETFKLRFVKLTLLLNAIVLLVAIGAFVLIRFHSPLGIA
                     VGLALLAVAVITYMYFSRFYQATKEWLETNADRGKEKD"
     gene            14232..14525
                     /locus_tag="VKO45_02965"
     CDS             14232..14525
                     /locus_tag="VKO45_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04868.1"
                     /translation="MLEKLKGDIRLLDRHIGVMKAVMEHEPIGIVKLSLLMDLPYHRI
                     RYSLRVLEQMGYIQASPAGAVTTAQARRLLSNLGHDVDELVDLLESLKEKKAP"
     gene            14585..14657
                     /locus_tag="VKO45_02970"
     tRNA            14585..14657
                     /locus_tag="VKO45_02970"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14618..14620,aa:Asn,seq:gtt)
     gene            14670..14744
                     /locus_tag="VKO45_02975"
     tRNA            14670..14744
                     /locus_tag="VKO45_02975"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14705..14707,aa:Ile,seq:cat)
     gene            14720..15778
                     /locus_tag="VKO45_02980"
     CDS             14720..15778
                     /locus_tag="VKO45_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04869.1"
                     /translation="MRSNRIGPIMQCPVCGQDCVQTAQDLVATLETVFAPCGECLGNT
                     LDKARPPPDLTIAKPCPCGKRFIDDVFVHIYGIMLEEGVLSREDPLKRVGMPLVHPGF
                     AMEAPPHLPANSLLLLSPVVDEPVAKRLHREVPEIRGVVKCGNFVPGAIDVDLTSSPR
                     VYELLAGCDVRADVFYTQHSPVVVYKQQSLIHIEFPRGYDPKIVNVGVNLRHSLPRAF
                     VDASCGAGTLGIIAAQYGVPHIIMNDAWYAAAFWAAFNIGVNRESLVAGELHMLEDYA
                     SMASHPVVREPRKIAYTEGKQLIEVYQGDFRNLPPLIPEREKILTVIDLFEKGDPGLN
                     EGIKKEWRERVGGEVFIP"
     gene            15794..16106
                     /gene="ffs"
                     /locus_tag="VKO45_02985"
     ncRNA           15794..16106
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VKO45_02985"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            16119..17069
                     /locus_tag="VKO45_02990"
     CDS             16119..17069
                     /locus_tag="VKO45_02990"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HMA04870.1"
                     /translation="MTTVSILGASGRIGSFAAHAISHDPHVGEILLIGRDGSEDRLDG
                     LARDLQDSFAARGSDIRIGWSVDLEDLRGSDIVVFTAGIPRHSGQDRLDLAMENARIV
                     APAARAVGRVAPDAIIFMVTNPVDVMTTVALKVSGMQPERVFGLGTHLDSMRLKSLIA
                     DFFRVHVSEVHTRIIGEHGESMVPLWSATTIGGIQVENLPAFSSLPREEMISTVKTSG
                     DEIIRKMGATTYGPGEAIATLVHTILGNENRILTVSRYIVEDLDGTGDVCIGAPARIN
                     RNGVFPVPIRIEESEAEAFRCSVEKIRGITSQVLDRLGME"
     gene            complement(17693..17953)
                     /locus_tag="VKO45_02995"
     CDS             complement(17693..17953)
                     /locus_tag="VKO45_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HMA04871.1"
                     /translation="MGKVVALVRIMPESVDVDMKKLEAELRKKLPSIQDVGVEPIAFG
                     LKALKVAVVVEDTEGGTEAVEKALAEVPGVSQAETVCVNRMI"
     gene            complement(17957..18118)
                     /locus_tag="VKO45_03000"
     CDS             complement(17957..18118)
                     /locus_tag="VKO45_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HMA04872.1"
                     /translation="MEIKKCTSCSAPLAEEGATEFGCPECKALIRRCVRCRMQSVPYV
                     CPKCGFWGP"
     gene            complement(18164..18763)
                     /locus_tag="VKO45_03005"
     CDS             complement(18164..18763)
                     /locus_tag="VKO45_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HMA04873.1"
                     /translation="MDLSGRPLVVKVGGSLFTRAAGIVSSLKGAHRPLLVIPGGGPFA
                     RMVRSLHLPNEPSHWMAILAMDEFGWYLAAGGIPVTRELSRPRGLEILLPYRVLRERD
                     PLPHGWDVTSDTISAWVASELGLDLLILKSVDGITRDGSLVPRVTEPFPCSEVDPCLV
                     PFVLSRQVRTTILNGRAEGRVRAFLEGRAVPGTVIEPRL"
     gene            18817..20493
                     /locus_tag="VKO45_03010"
     CDS             18817..20493
                     /locus_tag="VKO45_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HMA04874.1"
                     /translation="MRRILLVLQLLLVLVLVFQPTACLRIVEFCPDTYLPRDADAYFV
                     MDGVGSLSNITISDGEGSLAFPAGARIRGRVTVADEGLAYRTTHGGFPDFELKNTTPD
                     VPDMVKTGRYAPANDGDELILFLNGTAVQSIRWPGDLMPREGQVHVLTETGWDPRILL
                     LGQSEFSPVTFVNATVTAYVSPDSSYGVIGETVTRARQEILVNAYELTHPGIGDLLAA
                     ARQRGVQVTVLLEGGPVGGISREEAAVCAALNRSGIPLYRMTTTAAAHARYRYNHAKY
                     LVIDGENLLISTENFGIHGYPPTGSRGNRGWGALIEDPGVAGYFREVFLADRRGGDVV
                     PYIPSPGNVTPPFLEPYRVEFPSLRVTGVTVTPVLSPDTSGEIFFLFGRARNRIDIEQ
                     ASIRNTSGGELDPFLSAAVNASRRGVAVRVLLDGSMYNDEGPADNDEMADLVNAIALG
                     EGLPLSVRILEPEAGNLDAVHTKGIIVDRQIVMISSINWNANSPNFNREAGVILESPA
                     LGDYYSAVFEKDWSLAGKRAGLPGPDLVKIAIAGAVILVLLIALYCRKRR"
     gene            20757..21584
                     /locus_tag="VKO45_03015"
     CDS             20757..21584
                     /locus_tag="VKO45_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04875.1"
                     /translation="MEFLITGRIRETESKRPVPGLFVRAADKDLLYDDILGNATTKPD
                     GTFAIGYEGSDFRELFERKPDIYLVIYGSETAMDPGRPRDRPLFSTRGHVRFNAGRRE
                     HFEILIPRESLGNDAPGRVIVTTPGSGAWKEKIDEYHHMHPANYQVDPKRGFSAPYLK
                     CSSNFGPEIRNLKVGDSGWVTVRVSNSGNGVAGTTYVAVYEGPAGYSSPLGDYRINDY
                     RIECVCPGQTVDVKLYWTRLKEIGRIVGICYDPFLDPRGFTVVEQYNRHITSIHYLS"
     gene            21597..23471
                     /locus_tag="VKO45_03020"
     CDS             21597..23471
                     /locus_tag="VKO45_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HMA04876.1"
                     /translation="MPKYGIHHIVLNEAVTQLKVSGHAAGVSAAAIIDAERASAVIGS
                     IGPDLFFWGTDYEYVDKLYTLYENIEEVVEIYNSIVQPIRDVINAVGEPVEDLVETLA
                     PNTVELIKRVLEEIRETASLFKSAISTQLLAGVLEVGDFFSETAKVGPLAQQFFQLYV
                     PDLQSNKPEDDWYWFDMLHYRHTGEFAAKLVKNAQTNRQKAFAFGYISHIATDIAGHP
                     FVNQVCGSTYRMNVWRHVTAENFMDTWKFAQYYTDTDGNGESINQTLLSHLNIPEALP
                     SDIGDLLYQCFIDTYGNYPHPTNRLPGGFYTREDIDKTYEVFYKVLKFMEDQAVKRPE
                     EPFSGVGDVLSDALDAFRPPPAPPSSSSAACSWEDILSFGITESSRECYENFFEEVET
                     WLDYLGELLEWTIETLRNLIDLLLALLLSLPISVLLAILYGIQLLCYELYRNARMVLS
                     TMGFVFPEPYELNTSIARNLITPTECFSLNFKAFPSIGKPARTNLVCPVTEAEQPSTA
                     AGFHPGTIDATPERFISIEPFDVDHLAMYSSAGTPTSTLSLEHDRLTFGNATDLTAWM
                     IENANDPTVLSGTSKIYPILYTNWNLDADRGYGYKDWKGDVPQDIPYVVSNESYKTED
                     "
     gene            23650..23874
                     /locus_tag="VKO45_03025"
     CDS             23650..23874
                     /locus_tag="VKO45_03025"
                     /inference="COORDINATES: protein motif:HMM:NF021362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HMA04877.1"
                     /translation="MREGAFKEYLRRSVVKAVSYRVVVVIADFAAVYLFTNRLEIAVG
                     FVLVSNVYTSVLYFLHERFWDRVAWGRGVG"
     gene            23922..24284
                     /locus_tag="VKO45_03030"
     CDS             23922..24284
                     /locus_tag="VKO45_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04878.1"
                     /translation="MLERQERTALLLLLAILAILLAGHLILTGLGNAPFAAPYGDGSR
                     EGDLVLLTGVVGTVTPTSTGGHLLLTVNGTRVFIPSPLSRQVSLRVNDTVRIYGIVQA
                     YQGLREVAVRDARDVAKE"
     gene            24468..26552
                     /locus_tag="VKO45_03035"
     CDS             24468..26552
                     /locus_tag="VKO45_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMA04879.1"
                     /translation="MAEQHRQTKDYFWFVVIFGTTGAALTATLIGLNRGAFAVYPHLY
                     YFPIIIVSYLYPRRGVIFSVLLGGAYLFLTYAFAFLYPSVVTGDVLAAGTVHFYVFVT
                     VGFIASSLSWKLQESETKFRDITENSPLGIYLTEKGKFRYVNPRFAEIHGYGPDDLPG
                     RVGLENLVHPDDWQVVREQFHRLDAGEEKPFKSLVRTQTKDGRTIWVELLGSRITYQG
                     RPAYMGTLMDVTELKQTRDALAESEARLRTLIESIGAGVLVIDPVSHLITDVNPAAAG
                     MIGSPREEIVGKACHRFICPAEEGHCPVTDLGETVDHAERVLLQADGNKRPIIKTVVN
                     VNLGGKPFLLETFLDISERQKAEIALRESEKRYRTLFDSASDAIFIQDLSGHFLEVNR
                     AACDQLGFTREEFLKMDPRDIEAPPFKGLLMDWIEEVKNGGFLEMMRGGRSTFDTVHV
                     KKDGSYMPVEISCRFIEFENAPALLLIARDITERRRMEGIRALLASIVQSSEEAIVST
                     ELDGTVVSWNPGAEAIYGYTEGEMKGQPMSRIVPPELHHEMIDVLDRLAHGERIQHHE
                     TVRLRKDGSRVEVSLSVAQLKDAYGQVIGTATVARDITQRREAERALLAFINEAAMRL
                     RHPVEIVHDQLKEMQEQVGTGEMTPEDLRILLSVQIKNTEQIVENLNYLNRAILQGKD
                     DIPDSHRRYLTR"
     gene            26563..27267
                     /locus_tag="VKO45_03040"
     CDS             26563..27267
                     /locus_tag="VKO45_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04880.1"
                     /translation="MTFSFDRLDKDKIYLCLTSAERLRENSSTIIKGLLSKGYVVVVV
                     TTNQPYAILKKTYEKDGIDLSRVGFIDAITRYAVGEVPPGAKGVRFTSSPSDLTQLGI
                     AISDSLKDMSGRKAAILLDSVSTMLIYLPSTNLSKFIHFISSKLKILDMAGVFLAVED
                     GLDPTLLSQLTTFVDQVINMDREAAAPAPSPAPPSPPPSPPPRPPSSGTPPPPPAPPR
                     PSPQLPQDPYRRPPPG"
     gene            27335..28267
                     /locus_tag="VKO45_03045"
     CDS             27335..28267
                     /locus_tag="VKO45_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HMA04881.1"
                     /translation="MDVAAILLTIAGLCLFETISSIDNAIINAEVLSTMGERARRWFL
                     VYGLIIAVFLIRGILPWAIIWFTTPSLGPVGALTAAFSDDPLVLDAIEASAPILLMGG
                     GTFLVFLFFHWLFLEEKHFGLRGERYIQKKGVWFFAVVSVLLTLIVWFALGVSPLVAF
                     GAVVGSTAFFIVHGFRQNAEFQEKSLMDRSMSDISKIFYLEVIDATFSIDGVLGAFAF
                     TLSVPLIILGNGLGAFVVREITIGNIDRIKRYIYLKNGAMYSILALGSIMILDSFGVH
                     IPSYLSPLITFGIVGYFFYRSVLVARALEGSPGD"
     gene            28311..28970
                     /locus_tag="VKO45_03050"
     CDS             28311..28970
                     /locus_tag="VKO45_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04882.1"
                     /translation="MHLRVPFLVITLLLSLALLTGCSSPPASQSPGTPVTTPPPSTEP
                     PVTTATVIPTTAPPEPYPGALSIGTPYRYGREDIAMEVTVYRAITMDGYEWWSTAWGK
                     YWNTTPDEGDHFLFAFVRLIDRGTARARLPSRSMFVLHGGDTSYVQNVDRDPSLWIKG
                     VGVRQYDFSYDTSAGWIDPGESNKVEGFLLFEVPATLEPGDAHLEVTFSSQADAVWKL
                     G"
     gene            complement(28927..29394)
                     /locus_tag="VKO45_03055"
     CDS             complement(28927..29394)
                     /locus_tag="VKO45_03055"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HMA04883.1"
                     /translation="MEKREKQRSKVYLAAPLFSDAERAYNLVLRDLLESHGCRVYLPQ
                     ETGEGLAGPERDQAIFRSHRKALEGASCVVAICDGVDTDSGTAWEMGFAAAKGIPVIA
                     LSTDRRRPWAGKKVNLMIRQSAEVVGTPEEILTALDQGCSSRASRRRPPGSRR"
BASE COUNT         5364 a         9446 c         9349 g         5496 t
ORIGIN      
        1 aagaggccga ccgcgagggc gatcttcccc gggacatccc gcatattgat gggatagagc
       61 tgggccttcc gggtcgcctg catctggcag cagacgcccg tgatcccgac cttgtcgaga
      121 ccgaaggacc gggttgcctc cttgagccag gagatctggg ggctgatgtt gtacttcgtc
      181 ccctgggcct tgaggatctc ctcacggctc atggcgacca taggccgggg ctgccagggc
      241 cggtcgccct tgcccgccac gatggccccg tcgatgatcc cctgttcgag ggcatagacc
      301 atgagggcgg tggcgatgcc gccgtcctgc gacttctgga ggatctcctt atcggtgctc
      361 cgcgccgcga cacaggtcgt gtatttgccg agatatggat ctgatgccat ctctctcacc
      421 tcacttgagc gctccagcga tgatctcgct gatgccctcg aattcaggaa tcacgtcgaa
      481 gttgaagaac gacctcgggc aggagccgta gcaggaaccg cagttgatgc agaggtcccg
      541 ctgtccctgg ggcttcccga actcgtgcgt aatggcccgg acagggcagg ctgccgcgca
      601 gataccacat ccgatgcaga gtccctggtc gacgacgtcg ttcatcaggt tgttgtagga
      661 tgccttcgtg gtctgcttcg cgagggccat gagcggcttt aagtacttgc ccgcgagggc
      721 cttctggtcc gcgttcccct gcaggagcag gtacgccatg actgcgacgt tcctgatcag
      781 ctccgggctc ggggggcatc cggggatgta gacatccacg tcgatgaggt ccccgatggg
      841 gaggaaggac tcgtgctggg gctggttgtg ctgcccgccg cggcagaacc gggtgatgtt
      901 cccgtagctc gcgcatgagc cgagggccac cacgattttg gacttcttcc gcgtctcctt
      961 gatctcctcc accgattcct tgtcctggat gcagatcgaa ccctcgacga gggccacatc
     1021 catctcgggg atgtgccgga cgtcggcgag cgcgatgccg tagacgaggt cggcatagtc
     1081 gtccaggatc ttgatgagtc ccaggtaatt gtcggccacg gacacgaggc agccggtgca
     1141 gccgctcatg tgtacgtgcc cgagcttgat cttgtttgcc acaggtttcg cctcctttgt
     1201 agcttccacc ttcttctccg tcgcaacccc ggcgggcttt tcctctccgc cgatggtctt
     1261 cttgatggag ttaagcagtc ccatagttca tcccgatttc cttgagtatc tcggatacag
     1321 ctccaggtat ggatctctga acctcgtctg tgatcccgat gaccatctca ggggcagata
     1381 cgtacttctt ctggcatgca aggacccgga tccggatatc gtccttgatg cgctggagcg
     1441 gctcggtcag gtcccaggag tgggcgtccc ggtagctccc cacggggagc tcgtccacgg
     1501 cgagccacct gagctcgccg ggttcccccc cgaagtccgc gatgtccacg atgatgagct
     1561 tcttcgtgga ctccgggtcg aggagggtaa agacgaaatg ggggcccccg agccccgcgt
     1621 caatcacctt gacgttctcg gggagctcga ggccctggag actctccact accgcggttc
     1681 caaacccgtc gtccgcatag agcgggttgc cgcagcctgc caccacgatc tcgggataca
     1741 gcgatgcttc cattcccttc actggatgag cttctgggct accagcctgt tctcctggtc
     1801 caccacgatc atgtgggttg cgcacgacac gcacgggtca taggcccgga cgaccatctc
     1861 ggcgatctgc cagggcgctc ccgtgagacc gcggctgcag gtcgggaggt tccaggtggt
     1921 gggcacgagc atctcgtacc agagcacccg cccgtccttc acgcgtgcca ggtggacatc
     1981 ggttcccctt ggggcctcgt tcgcggccca gcccatggac ccgtcaccct gggggatgtt
     2041 gtccgccagg accttccccg aagtgtccag gttgtccagg cacttgagga tggagtagag
     2101 gcagtcgggg tattccagct gccgggcgat gtgctgggcg aaggtgccct tctcggggaa
     2161 gttcttgaag gtcgagagcc gggccctcgg gccgacctcg accggctggc cgtcgtatgt
     2221 cgggacgccg ttgcaggcct ccatctgggg gttgaccttc gtgccggcct tcgtggtccc
     2281 accgatgggg tacgtcgggt cctcgaggtc cacggtcatc tcaccctggt accagtccca
     2341 gggccgggtt tccttccacc gggcaaagtc ccacatgggc ttctcgtcga gggaggagga
     2401 tccgtacatc ggggaggtgg ccatgacgcc ctggtcgtgg aacccgagcg tcttcgggag
     2461 ggggatcttc ttccccgcga cgtcggtcca ctccctcttc tgcatgttcc ggatgaccgc
     2521 gatcatgaaa tccatctgct cgcgggcgag gaccaggcct tccttggcga ggtctgccat
     2581 cttctgcttc gcccgggggc tcacgttgcg gtacatcccg ccgacccggg ggttggacgg
     2641 gtggacggct tcgccgccgg cgatggcgcc gatggtctgc ccgatctcgc ggagccggat
     2701 gatccgtgct gcaacactcc tgaccggctg ttccttggtg aacgggttga tctttgtctc
     2761 cgttcccggg atatagaagt ccggaaggat caggatgttg tgcagggcga tgctgtggag
     2821 ccggttcgcg gcatggagga tgacccggag gagctttgcg tccatcggga tctcgcagcc
     2881 gactgaggcc tccatggatt caatacccgc caggcagtgg gcgatgggac agataccgca
     2941 gacccgagag gcaatcttcg ggacctgttc catcgtctta ccgatggcga gcttctcaac
     3001 acccctcacc ggggtgatgg agagccagtc tccgcgctca acgattccct gatcgtcgac
     3061 cttcagggtg agcttggagt ggccttcatg tctcgtggtt ggagagatct ctacaacttt
     3121 cgacaatggt atcgcctcaa ttttgcgttc ggtgttcttc aggctgtact gtggttaagg
     3181 cacactctgt gcgattgttc taagatagtt acccgtacat gttgtacgtt aagcacctac
     3241 tttctccgat gcgttagtta tagttttcgt tttggaacat cgcctgagtt ctgcttcaca
     3301 actgtgaaga aaaaagagtc ccgggtgcag gtgtgcccgg atggggccgg gaacgtccgc
     3361 gggggcatgg aacctgcatc acggtccgtc gcggagaatc cggttcgtgt gccggtgagg
     3421 aatctcactg gccggagagc tcgccctcca tctccaccat gagctcggcc agggtggcct
     3481 tccgctcggc ataggcattg tgctggtgga ttgactcctc gttggtctgc ttgatggtga
     3541 ccaccgagtc ggcggggaga ctcgcgaagg tgccgaggac cttccgggcg atctccctca
     3601 cgcagtcctc cacgaacctc gggttcttgt gggcctcgag gaccacgtag ctctcgtccc
     3661 cgcgcttcag cagttcgtag atccgggccg acatggagtc cttcaggatg tcgatgatgt
     3721 cctcgaggcc gaccctctgg tcgtcgtcgg tctcgatgca gaggaatccc ttgccccgct
     3781 ggttgtgggt ggccatgggg acctcggtga agaaggcgtc caccttttcc tgggggatcc
     3841 cgaggttcct gaggacatgg gcggcccggt ccttcatgat atcctgggcg caggggcagg
     3901 cggtcatgcc cgtcacctcg gccccgatgg acttccggac aaaggggttc gagtcgttcc
     3961 ggcgggccac ggcctttgcg tacaccatca ccacctcgtg gcaggcggtc atgctcacgg
     4021 gggtctccct gcgctgcatg aactggctcc gcatggtcac ctcggtccgg tcggcgtact
     4081 cgtgccggtc gaggagcttc cgggcgacgg tggagcagag ctgctccacc tccttcacct
     4141 ccccgtcgat ggcctgctgg aggacctcgt cgatgacctc gaagttccgg gagaggttcg
     4201 cccccttgag ctgcgccggg aggtccacga agacatcgaa ggtggagatg aagatgaccg
     4261 ggcgcttgtt cggcctcggc acctccacga gcttcttcac gttcgtcacg cccacccggg
     4321 tcaggttgat gcagacctcc ggctgggaag cctggacatc gggaaggtcc gaagaagttt
     4381 gcgtcatgga gagagaacga tcagtatgag gggaatgggg atgattcgct aatgaatctt
     4441 cccttttcct catataatct ttatctcgta ccacgcgaga taactgcggg tatggaaccc
     4501 acgataccca ggtactttga tgatgatgcc gatctcggcg tgctcaaggg gaagaccatc
     4561 gcggtcatcg gctacggctc ccagggccgt ggccagtccc tgaacctccg ggacagcggc
     4621 ctctcggtga tcatcggcct ccggcccggg aagagctggg accgggcgaa gaaggagggc
     4681 ttcgaggtcc tcccggtgaa ggacgcggtg aagaaggcgg acgtggtcca gatcctggtt
     4741 cccgacgagc tgcaggcggc ggcataccgg gctgatatcg gcccgaacct gaagccgaag
     4801 acctgcctca tgttctccca cgggttcaac atccacttcg gccagatcgt ccctcctacg
     4861 accgtggatg tgatcatggt ggccccgaag gggcccggcg ccatggtgag gagaacctat
     4921 gaggagggga agggagtccc cgccctcatc gcgatccatc aggatgcctc ggggaaggcg
     4981 aaggacatcg ccctcgccta tgcgaagggc atcggcgcga cccgcgccgt cgtccttgag
     5041 accagcttcc gggaggagac cgagaccgac cttttcggag agcaggcggt cctctgcggg
     5101 ggctgcacct ccctcatcaa ggccggcttc gagacccttg tcgacgccgg ctatgccccc
     5161 gagatggcgt atctcgagat cctccacgag ctgaagctca tcgtcgacct gattcacgag
     5221 gggggcctga ccaacatgcg tgccgccatc agcaacaccg cccagtacgg cgacctcacc
     5281 cggggccccc gggtcatcgg gcccgaggtg tacggggtga tgcaggagct cctcgacgag
     5341 atccagtccg gcgagtttgc aaaggagtgg atcctcgaga accaggtgaa ccggccggtc
     5401 tttaacgccc tcacgaaggc cgacgaggag cacctcatcg aggaggtcgg gaaggacctc
     5461 cgcagcctca tgccccagtt caggaaataa tgtctttcga cattatttcc ttctccctcg
     5521 gagattgtta tctccttcgg gttcaagaaa taatttcctc gaaattcctt tttctccctc
     5581 ggagcctgat ggcccctccg ggttcaggaa gtaattccac ccatcccgtc caacccaccc
     5641 ttttttctcc atcgccctcc aatcaccctc accatgtccg tctgcatcgt gtaccactcc
     5701 gagaccggga acacgaaaat ggtggccgag gtcgtcgcaa aggcgacggg cgcggtcatg
     5761 atcccggtca gggacaccgc gaattacggc aagatcagca ggtatctcgt gggggcccgg
     5821 aaggccatgg ccggtgagaa ggccgtcatc gagccatcga agatagatgt ctcggcccac
     5881 gatctcgtca tcgtgggcac gccggtctgg gcatggcgcc cgacgccggg tgcgaacgca
     5941 gccgtcgaag ccctcaccgg ctgcgaggga aagaaaggga tggtcttcgc cacctgcggg
     6001 ggaaagcccg gggaaaccct cgggattttg aaggccgccc tcgaagcccg gaaggtgaag
     6061 gtcgagggcg ctttttcctt cgcgaagaga gacctggggg acgcgaagaa gatcggggag
     6121 ttcatcgacg cggtgaagaa ggcctcgggg taagcccgaa gaggcatgtc aatgcctcga
     6181 gggagaggga aatgtcgatt tcccgacccg gaggggcatg tcaatgcctc cgacgggatg
     6241 tctcttcccc gaaggagagg cagtaaattt cccgcctccg agggagaggg aaattctatt
     6301 tcctgagccc ggcacgggaa ggaaaaatca ggagcgcatg agccgggtga gggatgtgcg
     6361 ggcgctctcc atctcctccg gcgacagggt caggctctcc gttcccgccc ggatgcggag
     6421 cccctctccc cccgcctcgc cgatgacccg gtacgacaat cccttcaggt cggactcctc
     6481 cttcaccgcg aggaggaacc tccccgccga ctccgagaag agctcgcaga gggcatccgg
     6541 accgagaacg acctttgccg acgggaccag agcggcgagg gcggagagga gacccccctg
     6601 ggagaggtcg gtcgccgcct cgcaccgatc gccggccacg agctcccgga tcgcgttgat
     6661 caccccggga tcggccaccg agggggggtc gccgccgcac ccggccaccg cgtccaggac
     6721 ggatcccccg tactcgggtt ttgtctcccc gacgagggcg aggagggatc ccgccggtgc
     6781 cgccttccac cgccgcacct ctccctttcc gaggaggccg atggacgggg tggggaggat
     6841 cttcgtaccg aactcgtcgc tctcgttgta cagggataca ttccccccga cgacggggat
     6901 cttcagcctc ctcgccatgt ccccgaggcc gaggatgcac tgctcgatct gccagtagat
     6961 ctcggggtgg atcgggctcg cgaagttcag gcagttcacg atacagagag gggtcgcgcc
     7021 gagggccgcg aggttcgcgg cgttttcgag gagggagttg gccgtcccct cgtacggggc
     7081 aagggcgatc tgccgggggt tgcagccgca ggagaggacg agggcgccct gcccgagccg
     7141 gaggaccgcc gcatcccggc cgagggagat ggaccggagc tggacgtcat ggtcgtactg
     7201 ctccgtgacc cagtccttcc cggccacttc ggggtgggag agcaccgcga gggcgaggtt
     7261 cttcaggttc gcgaccgggg ttctgaaggg cgcccgggcg ggggcgggcc tcttctccca
     7321 gaaacagagg ggggcatcgc cggcgaggag ctggatgggg aggtctgcca ccacctttcc
     7381 cctgaaccgg atgatgtacc ggcactcggg gatcacctcg ccgatatcgg accagccgag
     7441 gtcatacttt tctgcgatgg cccccatggc cggcacgtcg gccgccgcaa cctcgaaaag
     7501 catccgctcc tgggactcgg agagcatgat ctcgggcggg gtcatccccg cctcccgcag
     7561 gtggatccgg tcggcgtgaa tgagggcccc gaagctcgcg cacatctcgc tcgacgcccc
     7621 ggcgaggccc gccgccccca ggtccttgca ggagaggacc tttcccgtct ccgccatctc
     7681 gagggtggca tcgataatga gcttctcggt gtacgggtcc ccgatctgga ccgacggccg
     7741 atcctcggcc tccgagtcct cggagaggtc ccgggaggcg aaggaggctc ccccgaggcc
     7801 gtccctgccg gtggatgacc cgacgaggag aaggtgggac cccggccttt tcgcccgggc
     7861 ggtgaggtac cggtcggggt ggacgatgcc cacgcagacg acgttcacga gggggtttcc
     7921 ccgataggac gggtccaccc ccatctcccc gccgaccacg ggcaccccga tgcagttccc
     7981 gtagtccccg atgcccgcca ccacgtgctc gaagaggtac cggttcttct catgctcgaa
     8041 ggtcccgaag taaaggggat ccaggagggc gatgggccgg ccccccatgg ataccacgtc
     8101 cctgacaatc cccccgaccc ccgtcgctga accatggtac gggtccacat agctcgggtg
     8161 gttgtggctc tccatcccga cgacgacggc aaggtcctcc gagtacctca ccaccgcggc
     8221 atcgtccccg ggccccacga tcacgtcctt cccctcggtg gggagggtcc tgaggagggt
     8281 cttcgtcgac cggtagctgc agtgctcgct ccagaggttc tcgaagcatg ccatctccac
     8341 gtcggtgaga tcccggccca gttcccgccg gaggagggag agatcctctg tctgcatgta
     8401 cggaaggttg gcgaggggtt gagataagta tgactgtcgg gcgccgggga atagggatca
     8461 aagggccccg gggggtaacg gttccagggg caccgggagg gcgatctaag gcaccggggg
     8521 gtggcggatc taagggcccc gggggtggcg gatctaaggg ccccgggggc gagctttaac
     8581 accggtgcgg ccaaatacaa tacgcatgcc ccagtcgtac gaggacctcc tgaagaaggc
     8641 ctactcccag atcaccgagg tggagagctc ccacgagcgc ttccaggtgc cgcccgggaa
     8701 ggtcttcatc gaggggaaga ccacggtgct cgagaacctc gcggagatag cggacatcct
     8761 gaaccggagc cccgaggatc tcctgaagta cctggtcggg gagctgggca ccgccgggaa
     8821 aatcgagggc aaccgggcgg tcttcaacgg gaagttcgag cccgcctcgg taaacgctct
     8881 catccggagc tatgtcgagg attatgtcat ctgctccgag tgcgggaggc cagacacccg
     8941 gctcgtcaag gacgggagga tccccatcct ccggtgcgac gcctgcgggg ggcaccggcc
     9001 ggtgaaaaag cggaaggtcc ggctcgagcc ccaggaggag aggccccagg aggggaccgt
     9061 cgtcgagctc tccatcctct ccgtctcccg gcgtggggac ggggtggccc ggatggggaa
     9121 gtacaccatc tacgtcgcgg gggcaaagcc aggggagaag gtgaaggtga agatcacgaa
     9181 ggtcgcgggg tcggtcgcct tcaccgagcg ggcacagtaa tggggaccgt gttcccgtgc
     9241 cgggaccggg aataaaaaaa cttttttaga gtgattcagg gagggggctc gccgcaggcc
     9301 tgctcaggct tccttgattc ccttcatgat ctcgtcgcag tagcccgaga tcctcttgca
     9361 ggcgtccctg atgaccttga tggggtcctt ccggttcgtg gtgataaaga actcggggtc
     9421 cgagtgctgg aactccatgg tgtagcgggc cacgtccacc gcggggtcct tgagaacctc
     9481 gtcggtgagg aggttcatga aggtgtgtcc ctccccgggg aagacgatcc gtgcccggtc
     9541 ctttgacagt tcgagtatct tgatctccat ctcacctatt ccttggtgag aggggaataa
     9601 aagaggtttg ggtaaggagg aggcgcgaag cggctcctcc tgtccagggg ggtaaggggc
     9661 ccctctggac tgacctgggt cccggaaggg acccggctcc gaaggagacg gggctggccc
     9721 gtggggatag tggccccttg ggaccatgcc gaagccgtcc agaggggaga aggcgagaag
     9781 cggctccccc ggtccagggt ggtaaggggt ccctctggac ccgggccccg ggtcccccgc
     9841 ttcacgtgaa gaggatgcat tccccgtccc gggccaggtg ggggagggtc caggggatga
     9901 ggtgggagag gtggacgagg cggtactccc ccgggtgtgc ctccccggcc atccgcaggg
     9961 cgtcctcgta gttcatgtgc ttgttcagcc ggatctcggg cgggacgatg gcatcgagga
    10021 tgagaaggtc gacccccgag aggagcctcc tcgaccgttc ggggatatct gacgtggtgt
    10081 cacccgtgta cgcgacggat ccccccggtg aatcaatcct cacgccgaag gtgggaaccg
    10141 gcggatgatt gacggggaaa agggtgaccg tgaggccgga aaactcgaag gggagaaagg
    10201 gttccacccg gtggccctcg aagggaagga aggcgaagag gggtgcgcag tacgagaggg
    10261 tctcatcggc tgcatagacc gggggagggt cctggacccg atagaactcg ccatagccgg
    10321 agtaatggtc gtaatgcccg tgggtccaga tcaccgcgtc gatgcccggg gagcccgcgc
    10381 agaggagctg ctggcggagg tccggggagg tatcgatgag gaggtgccgg cccccctctt
    10441 cgatgaggag ggaggtccgg aggcgctgga tccccgcttc tcgggcgtac ctgcaggtct
    10501 cgcagtcaca gcccaccttg ggggtcccga ccgcgtcccc cgtcccgagg aaccggagtt
    10561 ccatgttacc cggacggggt cccccggagc atgttccggt tcgtgacgag ggtaaacccg
    10621 cgctcgatgt cctccctctc gatccggctg ctttcccgga cgagggacga gaggagggcc
    10681 tccttgacga ggacccggag gtctgcaccg gagaagccct cggtcatccc ggctagctcc
    10741 ccgagatccc cgtcccaggg gagggagttt gtgaccttcg cgaggattgc cttccgcatc
    10801 ccggcatcgg ggaggaagaa ctcgactacc tcgtcgaacc ggcgccaggc cgcctcgtcc
    10861 aggagctggg ggtggttcgt cgccgcgagg aggaggaccc cgtcgcggac gaggcttacc
    10921 cggtcgatgt tcttgaggag catgttcacc gcccgcttca tggcgccgtg gtcatcggtg
    10981 atccggctct tcgccacgaa gtcgaactcg tcgatgaaga ggatggcggg ggcgatcttc
    11041 tttgcaaatt cgaagatacg atcgatgttc tttgaggtct cccccaggta ctgggaggta
    11101 accatggaga ggcgcacctc gagcatcggc atgtggagct cccgggacag ggcgagggcg
    11161 agggtggtct tcccggtccc cgggggaccc acgaagagga gcttcccgag ctcgtagatc
    11221 cggtgggagc ggaggaggtc ccggtgaagg agtgccgtcc tcaccttcga ggcgatggcc
    11281 gcctgctggc ccgtgcagat caggtccgag agccggggtt ccacctcgtc cggagcggac
    11341 acgatgagga gctcgagagc cgccttcagg gcctcgtccc ccacggtgag ggccgcgatc
    11401 ctcgcatcga ggcaggcccg ggtgtcctcg acccgggggt tccccgcctt cacgtcccgg
    11461 tacgagaccc ccgccgagtc gaagccctcg aagaagctcg cgagggccgg gttccgggtg
    11521 atctgggcct ttcccccctt cttcaggaac cagcctgccg cggcatccag ggccgagatg
    11581 cgcatccggt ggccgaagga gtccagctcg acgaaggggt tccccttcac cttctcggag
    11641 gcatccccga tcccggtggc ctgggcgacg gtcccgtccg agacgatgac gggccgcttc
    11701 acctgcccgt ccggccccat cccgaaggcc tccctgcagc gggggggcag gtcattcccg
    11761 tccaggtccc ggttccggtt gaagatctcg gcggtgagga ggatctccat cacctgcatt
    11821 acctcgttcg tcccttcagc agccataggt ataacttaga gaatttaccc ggaaagtcaa
    11881 taagggtatc tcaccgggtg gagacgatgc acccgtcacc ggtgacgata accgtgtgct
    11941 ccgcctggga aacgagggac cccggcacgt cccggaggac cgggtaggcg tggagctgtc
    12001 ccgatcggac gaggccggag aggctgatct cgagcctctc cccggtgagc caccggcgtg
    12061 cgaagggaag gccgtgccgg tccttcacct gttccaggat gcgcttcgcc gcggggagcc
    12121 gggcgggacg ctccaggacc tgctggaaga tctggaccct cgggccgtcg gagacaaaac
    12181 cggtacctgt cgatgcgaag ggctcgatgg caaagaccat cccctccttg aggaccgccc
    12241 ccccggagac ccggatgttc gggaccgtcg gcggggtgtg gatctcgtac ctccccagcc
    12301 catggccggt caggttcgcc accgggcgga agcccagggc ttcgatctcc gcctggatgg
    12361 ccgccccgag gtcgcccgtc gagacgccgg gccggacgag ggcgatggcc ttctcgaggg
    12421 ccgcccgcga cgcccggagg agcggcgcat tctccccgag gtccaccgtc cctgccgtgt
    12481 cggcgatgta cccttccagg tggacgccca ggtccatctt caccacatcc ccggccctga
    12541 agattcggtc atcgccctcg gaggcggtgt catgggccgc cgcctcgttc agggagagat
    12601 tcaggggaaa ggcaaggccc gccccttcac ccgtgacaag ggcctccacc tcctccacca
    12661 ccccgaggaa cggggcgccc gccctgacga gggggaggga ccgggagagg atccgggagg
    12721 cgatcttccc cgcctcgagg tacgtgtcga ggacctcgtc cttcatgccc ggagatccct
    12781 cggcatcttc gtgaggtcac ggaacccgtc cggggtcacc acccccatat cctcgaggcg
    12841 cacgcctccc ctccccgggt agtagaggcc gggctcgagg gtgatcacgt tccccgccga
    12901 gagctcgcca ccacccggcc cgaggaccgg gagctcgtgc acctcgagcc ctaccccgtg
    12961 gcccaggttg tggatgaagc ccctcgtgtt gctctcgaac cccctctccc tgaaataatc
    13021 gaccaccttc ccgtggacct ctgcacccgt cgccccgggc cggaccaggg tcgccgcgag
    13081 ggactgggcg ccgcgaacgg cctcgaacat ctcgaggatc tccggcgacg gatcgcccct
    13141 caccacggtc ctggacatgt cagccgtgta ccccgactcc tcttcctggg gaaagatatc
    13201 gaggatgatg ggggcatctt cccgtagcgc tcccgaaccc cggatatggg ggatcgccga
    13261 atcctcgccg caggagatga tggtatccac ggcccggcac ccgtgttcca gaagggcgac
    13321 cgccacctct gtctggagga tctcggaggt gaggggggtc tttccccggt agaggatccc
    13381 cttcctcgcc cgggcagaac ggatggagga gatggctccc cccatcgccg cctcggtcgc
    13441 ctcctggacc cgggcgatgg cggcgatctc atcgtctgac tttaccgccc tttgtttgag
    13501 gaccgtgtcg tcgtccaccg tgaaccggga gaaggcctcg agctcccggg cgagggcggc
    13561 cgggaactgg gggggcacga ggacgtcccc gccggcgatc ccggcgatca tccgggcata
    13621 ggccttccac cggttcttct cctccgcgag gatctcggtg aggccggcct gggcccgggt
    13681 tacggggatg gccgtggact cccgggcggc cctctcgtat tccatctggg aaacgatgat
    13741 ggtcccgggt tccccggact tcttcacgaa aaggacggga tcggtggtcc tgaaccgggt
    13801 gaggtacctc atgtcggcgt ccagggaaga cccgtatgcc acgtaggcct tcgccccggc
    13861 agcggcgatg gcatcgtcca ggctctccat ggggaggagg gttgacggca taccacaagt
    13921 acttttaccc cccgaacccc aagtacgagg gatggacgag catgcccggg agaccttcaa
    13981 gctccggttc gtcaagctta ccctcctcct caacgccatc gtcctcctcg ttgctattgg
    14041 tgcctttgtc ctcatccggt tccactcccc cctcgggatc gccgtcggtc tcgccctcct
    14101 tgccgtcgcg gtgatcacgt acatgtactt ctcgcggttc taccaggcga ccaaggaatg
    14161 gctcgagacg aacgcggacc gcgggaagga gaaggattga agtacaggga gcatgcaccc
    14221 tacatccagg gatgctcgag aagctcaagg gggacatccg gctcctcgat cgccacatcg
    14281 gcgtgatgaa ggcggtgatg gagcacgagc ccatcggtat cgtgaagctc tccctcctga
    14341 tggatcttcc ctaccaccgc atccggtact ccctccgggt cctcgagcag atggggtaca
    14401 tccaggcgag ccctgcaggg gcggtcacca ccgcccaggc aaggaggctc ctctcaaacc
    14461 tgggccatga cgtggacgag ctcgtagatc tgctcgagtc cctgaaggag aagaaggccc
    14521 cgtaacgttt cccataatgt ttaatacgcc tccgaaggat gtaataaagc atatcaagtc
    14581 ccgtgccgcc ataactcagt tggtagagtg gctggctgtt aaccagcatg tcacaggttc
    14641 gagtcctgtt ggcggcgctc tcctttcccg ggcccgtagc ttagtctggt gagagcgccc
    14701 ggctcataac cgggcggtca tgcgttcgaa tcgcatcggg cccatcatgc agtgcccggt
    14761 ctgcggtcag gactgcgtcc agacggccca ggatctcgtc gcgaccctcg aaacggtctt
    14821 tgccccctgc ggggagtgcc ttggaaatac tcttgacaag gcccgtcccc ccccggatct
    14881 caccatcgcg aagccctgtc cctgcgggaa gcggttcatc gacgatgtct ttgtccacat
    14941 ctacggcatt atgctcgagg agggggtcct ttcgagggaa gatcccctga agagggtggg
    15001 gatgcccctc gtccaccccg gtttcgcgat ggaggcgcct ccccaccttc ccgccaactc
    15061 cctcctcctc ctttcaccgg tggtggacga accggtggca aagcggctgc accgggaggt
    15121 cccggagatc cggggcgtgg tgaagtgcgg gaacttcgtc cccggggcca tcgacgtgga
    15181 ccttacatcc agtccccgcg tctacgaact cctcgcggga tgcgacgtca gggcggatgt
    15241 tttctacacc cagcacagcc ctgtcgtggt ctacaagcag cagtccctga tccacatcga
    15301 gttcccccgg gggtacgacc cgaagatcgt gaacgtcggg gtcaacctca ggcactccct
    15361 cccgagggcc ttcgtggacg cctcctgcgg cgccggcacc ctcgggatca tcgccgccca
    15421 gtacggcgtg ccccacatca tcatgaacga cgcctggtac gcggcggcct tctgggcggc
    15481 cttcaatatc ggggtgaacc gagagtccct cgtcgccggg gagctccaca tgctcgagga
    15541 ctacgcctcc atggcctccc atccggtggt cagggagcca cggaaaatcg cctacaccga
    15601 aggaaagcaa ctcatcgagg tctaccaggg ggacttccgg aatcttcctc ccctcatccc
    15661 cgagcgggag aagatcctca cggtcatcga ccttttcgag aagggtgacc ccgggctcaa
    15721 cgaggggatc aagaaggaat ggcgggaacg ggtgggcgga gaggtattta tcccatagaa
    15781 gggctaagga tatccgggga ctagcccggg tggcccgacg tcacctgtga cccgaaaccg
    15841 ccgacacgcg gggggcgaag tccgagggag actccggcga cctgctcacg ttttgtgcaa
    15901 cctgacgaag aagcctcgtc ctgtgaggtc aatggacagg gatcgaagct ccggaggggg
    15961 cggagacctg ccgccgcggg gaccggtcca ggcccggaag ggagcagact taccgcggac
    16021 atctggcgcc cacgggggag cggggtggag gagggctgca caggcggggg cgggacacgt
    16081 ggtggccacc gaggacgtgt ccccattgca cgattaccat gaccacggtt tccatcctcg
    16141 gggcctcggg aaggatcgga tccttcgcgg cccatgccat ctcccatgac ccccacgtcg
    16201 gggaaatcct cctcatcgga cgggacggga gcgaggaccg gctcgatggc ctcgcccggg
    16261 acctccagga ctccttcgcc gcccggggga gcgacatccg gatcggctgg tcggtggacc
    16321 tcgaggacct gaggggctcg gacatcgtgg tcttcaccgc cgggattccc cgccattccg
    16381 gccaggaccg gctcgacctc gccatggaga acgcccggat cgtggccccc gcggccaggg
    16441 cggtgggccg ggtcgcgccg gatgccatca tcttcatggt cacgaacccg gtggatgtca
    16501 tgacgacggt cgccctgaag gtctcgggga tgcagcccga gcgggtcttc ggcctcggga
    16561 cccacctgga ctcgatgcgt ctcaagtccc tcatcgccga cttcttcagg gtccatgtct
    16621 ccgaggtcca cacccggatc atcggggagc acggcgagag catggtcccc ctctggtcgg
    16681 cgaccaccat cggcgggatc caggtcgaga acctgcctgc cttctcaagc ctccccaggg
    16741 aggagatgat ctccacggtg aagacctcgg gagacgagat catccggaag atgggggcga
    16801 cgacctatgg cccgggagag gccatcgcga ccctcgtcca caccatcctc gggaacgaga
    16861 accggatcct gacggtctcc cggtacatcg tggaggacct cgacggcacc ggggacgtct
    16921 gcatcggtgc cccggcccgt atcaaccgga acggcgtatt ccccgtcccc atccggatcg
    16981 aggagagcga ggccgaggcc ttccggtgct cggtggaaaa gatccggggg atcacctcgc
    17041 aggttctgga ccgtctgggg atggaataaa tccccgttcg gattcaccgt cagaattttt
    17101 cctgggatct aggtctgaaa atctattcct gggccggtgt attctcaggg gaactactcc
    17161 gtcgcttgaa ggtggggtgg aggagggggt ggcgggtagg gttcagggtc tacgcatata
    17221 acagtgaccc acctgagaag cacttatgaa accaagacaa acggcgaatc aacccaaaac
    17281 ctgatctgag ctgtacggtt gcccgatgga cgctcctggg ggagggaggg ttcatcagaa
    17341 cccgacctta agcaacccgc ccacaggcgg ggtgcgaccg ggaggtttcg taagaaaccg
    17401 acctcgagcg acgaagtcgc gagtttcctc cagctcaccc ccgccgctct ggcgtccccc
    17461 gcatgaggat aggcctttcc gcgaactgcc ttcatggggt gttaaaaact gccgaatcag
    17521 gagtcccctg gaacgggggg ggtgcccgtg cggcgggggg cgggtgagcc cccccgagag
    17581 cacaccatgc aaagacatcg ccagttgtta ttcggccggg ggacgcgaat agttaccaat
    17641 gatgacgccc gaaaactatc tggaaaaaga ggagtgacga agccagttta tctcagatca
    17701 tccggttgac gcagaccgtt tcggcctggg acacgcccgg cacttcggcg agggccttct
    17761 ccaccgcctc ggtccccccc tcggtatcct cgacgaccac cgcgaccttg agggccttta
    17821 acccgaaggc gatgggctcg acgcccacgt cctggatgga gggaagcttt ttccggagct
    17881 ccgcctcgag cttcttcata tccacgtcca cggactcggg catgatgcgc acgagggcga
    17941 ctaccttacc catcccctac ggcccccaga atccgcactt cgggcagaca tacgggacgc
    18001 tctgcattct gcagcggaca caccggcgga tgagggcctt gcactcgggg cacccgaact
    18061 ccgtggcccc ctcctcggcg aggggcgcgc tgcaggaggt acacttcttg atctccattc
    18121 accgactccc aaaggattct ttataatcta gcccttcagg aaattaaagc cttggttcga
    18181 tgaccgtgcc gggcaccgcc ctcccttcca ggaaggccct gacccttccc tccgcccggc
    18241 cattgaggat ggtcgtcctc acctggcggg agaggacgaa ggggacgagg cacgggtcca
    18301 cctcgctaca ggggaagggt tcggtaaccc gggggacgag gctcccgtcc ctcgtgatcc
    18361 cgtccaccga tttgaggatc aggagatcga gccccagctc ggaggccacc caggcggaga
    18421 tggtgtcgga ggtcacatcc cagccgtggg gcagagggtc gcgctcccga aggacgcggt
    18481 aggggaggag gatttcgagc ccccggggcc gggagagttc ccgggtgacg ggaattcccc
    18541 ccgccgccag gtaccagccg aactcgtcca tcgcgaggat ggccatccag tgggagggct
    18601 cgttggggag gtggagggac cggaccatgc gggcgaaggg cccgcccccg ggaatcacga
    18661 ggagcggccg atgagcaccc ttcagggacg aaacgatacc tgccgcacgg gtgaagaggc
    18721 ttccgccgac cttcaccacg aggggccttc ccgacaggtc catgatcagg aatcggatcg
    18781 cgtccttctt aaaagggaag ggcatatccc acggtgatgc gccggatcct gctcgtgctt
    18841 cagctccttc tcgttcttgt cctggtgttc cagccgacgg cgtgtctcag gatcgtcgag
    18901 ttctgccccg acacctacct cccgagggac gcggacgcct atttcgtgat ggatggggtg
    18961 gggtccctct ccaatatcac catctcggac ggcgagggtt ccctcgcctt tcccgcgggg
    19021 gcccggatcc ggggccgggt cacggtggcg gacgagggcc ttgcgtaccg gacgactcac
    19081 gggggcttcc cggacttcga gctgaagaac accacccccg acgtacccga catggtgaag
    19141 acggggcggt acgcccccgc gaatgacggc gatgagctca tccttttcct gaacgggacg
    19201 gccgtccagt cgatccggtg gcccggggac ctgatgccga gagaggggca ggtccatgtc
    19261 ctcacggaga cagggtggga tccccgaatc ctcctcctcg gccagtctga gttctctcca
    19321 gtgaccttcg tgaacgccac ggtgaccgcc tatgtctccc ccgattcctc ctacggggtc
    19381 atcggggaga ccgtcacccg ggccaggcag gagatcctgg tgaacgccta cgagctcact
    19441 caccccggca tcggggatct cctcgctgcg gcacggcagc gcggtgtcca ggttacggtc
    19501 ctccttgagg gcgggccggt gggcggcatc agccgggagg aggcggcggt ctgtgccgcc
    19561 ctcaaccgaa gcggtattcc cctgtacagg atgaccacca cggcggctgc tcatgcccgg
    19621 taccggtaca atcatgccaa gtacctggtg atcgacggag agaacctcct catctcgacc
    19681 gagaacttcg ggatccacgg gtaccctccc accggctccc gtgggaaccg cggctggggg
    19741 gccctcatcg aggacccggg ggtcgccggc tatttcaggg aggtcttcct ggccgatcgt
    19801 cggggtggag acgtggtccc ctacatccct tcacccggga acgttacccc tcccttcctc
    19861 gaaccctacc gggtggagtt cccctctctc cgggtgacgg gggtaacggt gactcccgtg
    19921 ctctccccgg acacgagcgg ggagatcttc tttctcttcg gccgggcccg gaaccggatc
    19981 gacatcgagc aggcatccat caggaacacc tccggcgggg agctcgaccc gttcctgtcg
    20041 gcggcggtga atgcctcccg gcgcggcgtg gcggtgaggg tcctcctcga cgggagcatg
    20101 tacaacgacg aggggccggc ggacaatgac gagatggctg acctcgtaaa tgccatcgcc
    20161 cttggcgaag gcctgcctct ctcggtgagg atcctggaac cggaggcagg gaacctggat
    20221 gccgtccaca cgaagggcat catcgtggac cggcagatcg tgatgatatc gtccatcaac
    20281 tggaacgcga actccccgaa ctttaaccgg gaggcggggg tgatcctgga gagccccgcc
    20341 ctcggggact actactccgc ggtgttcgag aaggattgga gtcttgccgg gaaaagggcg
    20401 gggctgcccg gtccagattt ggtgaagatc gctatcgcag gggcggtgat cctggtcctc
    20461 ctcatcgctc tgtactgccg gaaacgccgg tgagggaggt tgccccagcc aggccctctc
    20521 gggagaattg aagagcctgc actgtgcggc gatctattca gggtttttcg tcgctatgtt
    20581 tttatatttc cggggagatt ggatttcgat cgcgacgaga tgagggcgga tccaatcatc
    20641 cccgcgaatc tccatcataa ccttccatga attttcttat accctgcccc ggggggagag
    20701 agcagggttt ccatctcttg cggtccgaga gggggatacc ctggtgaggt gattccatgg
    20761 aattcctgat tacggggaga atccgtgaaa ccgagagcaa aagaccggtt cccggtcttt
    20821 tcgtccgcgc cgcggacaag gatctgctat acgatgacat cctcgggaat gcaaccacga
    20881 agcccgacgg cacttttgcc atcggctatg aaggcagtga tttccgggaa ctattcgaac
    20941 ggaagccaga catatacctg gtgatctatg gcagcgagac cgcgatggat ccggggaggc
    21001 cccgggacag gccgttgttt tccacgagag gacatgtcag gttcaatgcg ggccggcggg
    21061 agcatttcga gatcctcatc ccgcgcgaaa gtctggggaa cgatgcgccc ggccgggtaa
    21121 tcgtgaccac gccgggaagc ggtgcgtgga aggaaaagat cgacgagtat catcacatgc
    21181 acccggcgaa ctatcaggtc gatccgaaac ggggattttc ggcgccgtat ctgaagtgca
    21241 gctccaactt cggcccggag atccggaacc tcaaggtcgg tgattccggg tgggtcacgg
    21301 tcagggtatc gaattccgga aacggcgtcg cgggaaccac gtacgtggcg gtctatgagg
    21361 gtccagcggg gtattcgagc ccgctcggag attaccggat caacgattac cggatcgaat
    21421 gcgtttgccc cggccagacg gtggacgtca agctgtactg gacacgcctg aaggaaatcg
    21481 ggaggatcgt gggtatctgt tatgacccgt tccttgatcc gcgcggtttc acagtcgtgg
    21541 agcagtacaa ccgccacatc acctcgatcc attacctgtc ataggaggaa gagaccatgc
    21601 caaagtatgg aattcatcat atcgtcctca acgaggccgt aacgcagctg aaggtttccg
    21661 gacatgccgc aggggtgagt gctgcggcaa tcatcgacgc agaacgagcc tctgccgtca
    21721 tcgggtcgat tggcccggat ctcttcttct ggggcaccga ctatgaatac gtggacaagc
    21781 tgtacaccct ttacgagaat atcgaggagg tcgtggaaat ctacaacagc attgttcaac
    21841 ccatcaggga cgtgatcaat gcagtcgggg aaccggtgga agacctggtg gagacactgg
    21901 ccccgaacac agttgagctc atcaaacggg ttctcgagga gatcagggag accgcgagcc
    21961 tctttaagtc cgcgataagc acccagctcc tcgcaggcgt ccttgaggtg ggggactttt
    22021 tctcggaaac agcgaaggtg gggccactcg cacagcagtt tttccaatta tatgtacccg
    22081 atcttcagtc caacaagccg gaggacgact ggtactggtt tgacatgctg cattaccggc
    22141 acacggggga atttgccgcg aagctcgtga aaaacgccca gacgaaccgg caaaaggcct
    22201 ttgccttcgg gtacatctcc catatcgcca ccgacatcgc cggccacccc tttgtgaacc
    22261 aggtgtgcgg gagtacatat cggatgaatg tgtggagaca tgttaccgca gagaacttca
    22321 tggatacctg gaaatttgca cagtactata cggatacaga cggtaacggg gagagtatca
    22381 atcagaccct tctttcccac ctgaacattc ccgaagcact gccctcggat atcggggatc
    22441 tcctgtacca gtgtttcatc gatacgtacg ggaattaccc ccaccccacc aaccggttgc
    22501 ccgggggctt ttacacaagg gaggacatcg acaagaccta cgaggtgttc tataaggtcc
    22561 tcaaattcat ggaagaccag gccgtgaagc gacccgagga gcccttctcc ggagtgggag
    22621 acgtcctctc cgatgccctc gatgccttcc ggccgccacc ggcgccgccc agctcatcct
    22681 ctgccgcctg cagctgggag gacatcctct ccttcggaat aacggaaagc tcgagagagt
    22741 gctatgaaaa cttcttcgag gaggttgaaa cctggctcga ctatctgggg gaactcctcg
    22801 aatggactat cgaaactctc aggaacctga tcgatctcct tctggcatta ctgctctctc
    22861 tcccaatatc cgttctcctg gccatcctct acgggatcca gctcctctgc tatgagctgt
    22921 atcggaatgc caggatggtc ctctcgacca tggggttcgt gttcccggag ccctatgaac
    22981 tgaacacgag catcgcccga aatcttatca ccccgacgga atgcttcagc ctgaacttca
    23041 aggcattccc gagcatcggg aaacctgccc gaactaacct cgtctgcccg gtcaccgagg
    23101 cggaacaacc gtccaccgcg gccggctttc acccggggac catcgatgcg acacctgaac
    23161 ggttcatcag catagagccg ttcgatgtgg atcaccttgc catgtattcg agcgcgggaa
    23221 ctcccacgag tacattgagt cttgaacacg atcgactcac cttcggcaat gccacggatc
    23281 tcacggcatg gatgatcgag aatgcaaacg acccgacggt cctttccgga acctcgaaaa
    23341 tctatccgat actgtatacg aactggaacc tcgatgccga cagggggtat ggatacaagg
    23401 attggaaagg ggatgtcccg caggatatcc cgtatgtcgt ttctaacgaa tcttataaga
    23461 ctgaagactg aaatccaatt tcttcctttt tcagggacaa ccccacattc cttctccata
    23521 ggacggcgac cgcccatcat gtcgtgtaga tcggggataa gcgtggcctg cgccggaatg
    23581 gtgggtcctc cttttccaaa tgccatatat acggcttaac gcaacccctc ccccggtaag
    23641 gtgtcggatg tgagagaagg tgccttcaag gagtatcttc gccggtccgt ggtcaaggcg
    23701 gtcagctacc gggtcgtcgt ggtcatcgcc gattttgccg cggtgtacct cttcacgaac
    23761 cggctggaga tcgcggtcgg cttcgtcctc gtgagtaacg tctatacgag tgtcctctac
    23821 ttcctccacg agcggttctg ggaccgggtg gcctggggga ggggcgtggg ttaggcgaac
    23881 ggatccacgg gatttatctt cccccccgtg aacctcccct cgtgctcgag cggcaggagc
    23941 gcacggccct ccttctcctt ctcgcaatcc tcgccatcct cctcgcaggc cacctcatcc
    24001 tcaccggcct ggggaacgca ccctttgcgg ctccgtacgg agatggttcc cgggaggggg
    24061 atctggtcct cctcacggga gtggtcggta cggttacccc gacatccacg ggaggccacc
    24121 tgctcctcac ggtgaacggc acccgggtgt ttatcccctc gcccctctcc cgccaggttt
    24181 ccctccgggt gaatgatacc gtccgcatct atggcatcgt ccaggcctac caggggttga
    24241 gggaggtggc ggtgagggat gcccgggatg tcgcgaagga gtagcccttc acctcagggt
    24301 atggctactc atccccgttc cgtgaaattg tccatccctg cctcacattc cacaggaaaa
    24361 gttcgttcac ccatgaaatt gaacccccga ccaaggataa aggattttag taccggcact
    24421 ttaactacta gtgtagaagc gaatccgtaa cccggaggaa ggcgattatg gcagaacaac
    24481 ataggcagac gaaggactat ttctggttcg tcgtgatctt cggcacgacg ggggcggccc
    24541 tcacggccac cctcatcggg ctgaatcgcg gtgcattcgc ggtatacccg catctctatt
    24601 acttccccat catcatcgtg tcgtacctct acccccggag gggggtcatc ttctcggtcc
    24661 tccttggtgg tgcctacctc ttcctcacct acgccttcgc cttcctgtat ccgagcgtcg
    24721 tgacggggga tgtcctcgct gccgggaccg tccacttcta cgtcttcgtc acagtcggtt
    24781 tcatcgcctc ctctctctcc tggaagctgc aggaatcgga gaccaagttc cgggacatca
    24841 ccgagaactc ccccctcggg atctacctca ccgagaaggg gaagttccgg tacgtgaacc
    24901 cccgcttcgc cgagatccac ggctacgggc ccgacgacct gccgggcagg gtgggcctcg
    24961 agaacctcgt ccatcccgat gactggcagg tcgttcggga gcagttccac cggctggacg
    25021 ccggcgaaga gaagcccttc aagtccctcg tccggaccca gacgaaggac gggaggacca
    25081 tctgggtaga gctcctcggg tcccggatca cctaccaggg acggcccgcc tacatgggta
    25141 ccctcatgga cgtgaccgag ctgaagcaga cccgggacgc cctcgccgag agcgaggccc
    25201 ggctccggac gctcatcgag tccatcgggg ccggggtcct tgtcatcgac ccggtgagcc
    25261 acctcatcac cgacgtcaac cccgcggccg ccgggatgat cgggtcgccc cgggaggaga
    25321 tcgtcgggaa ggcctgccac cggttcatct gccccgcgga ggagggccac tgcccggtca
    25381 ccgatctcgg ggagacggtc gaccatgccg agcgggtgct ccttcaggcc gatgggaata
    25441 agaggcccat catcaagacc gtggtcaatg tgaacctcgg ggggaagccc ttcctcctgg
    25501 agaccttcct cgacatctcc gagcggcaga aggccgagat cgccctccgg gagtcggaga
    25561 agaggtaccg gacgctcttt gactcggcga gcgacgccat cttcatccag gacctctcgg
    25621 gccacttcct ggaggtgaac cgggcggcct gcgaccagct cgggttcacg agggaggagt
    25681 tcctgaagat ggacccccgg gatatcgagg cacctccctt caagggcctc ctcatggact
    25741 ggatcgagga ggtgaagaac ggcgggttcc tcgagatgat gagggggggc cgctccacct
    25801 tcgacaccgt gcacgtgaaa aaggacggga gctacatgcc ggtggagatt agctgccggt
    25861 tcatcgagtt cgagaacgcc ccggcgctcc tcctcatcgc ccgggacatc accgagcgca
    25921 ggcgcatgga ggggatccgg gccctcctcg cctccatcgt ccagtcatcg gaggaggcca
    25981 tcgtgagcac cgagcttgac gggacggtgg tgagctggaa cccgggcgcc gaggccatct
    26041 acgggtacac cgagggcgag atgaaggggc agcccatgtc ccggatcgtg ccgccggagc
    26101 tccaccacga gatgatcgac gtcctggacc ggctggccca cggggagcgg atccagcacc
    26161 acgagaccgt gcggctccgg aaggatggga gccgggtgga ggtctccctc tcggtcgccc
    26221 agctgaagga tgcgtacggg caggtcatcg ggaccgcgac cgttgcccgg gacatcaccc
    26281 agcggaggga ggccgagcgg gccctcctcg cctttatcaa cgaggcggcc atgcgcctcc
    26341 gccatcccgt ggagatcgtc catgaccagc tgaaggagat gcaggagcag gtgggaaccg
    26401 gggagatgac cccggaggat ctcaggatcc tcctctcggt gcagatcaag aacaccgagc
    26461 agatcgtcga gaacctgaac tacctgaacc gggccatcct ccaggggaag gacgacatcc
    26521 ccgattctca ccggaggtac ctcacgaggt agcggagccc tgatgacgtt ctccttcgac
    26581 cggctggaca aggacaagat ctacctctgc cttacgtcag ccgagcggct gcgggagaac
    26641 tcgagcacca tcatcaaggg cctgctttcc aagggctacg tggtcgtcgt cgtgaccacg
    26701 aaccagccct acgccatcct gaagaagacc tacgagaagg acgggattga cctctcccgg
    26761 gtgggattca tcgatgccat cacccggtac gcggtcgggg aggtgccccc gggagccaag
    26821 ggggtcaggt tcacgagcag cccctcggac ctcacccagc tcgggatcgc catctcggac
    26881 tccctgaagg acatgagcgg ccggaaggcg gccatccttc ttgattccgt ctcgaccatg
    26941 ctcatctacc ttccctcgac gaacctctcc aagtttatcc acttcatctc gagcaagctg
    27001 aagatcctgg atatggccgg cgtcttcctc gcggtggagg acggcctcga tcccaccctc
    27061 ctctcccagc tcaccacctt cgttgaccag gtgatcaaca tggaccggga ggcggccgcc
    27121 cccgcaccgt ccccggcacc gccgtcacct cccccgtcgc ccccgccccg gcccccctcg
    27181 tccgggaccc cgccaccgcc accagctccg ccgaggccgt ccccccagct accccaggac
    27241 ccgtacagga gaccaccccc gggctgaaac cggccctgct ccccggagcg gggtgcgcac
    27301 gcttccgttg caccggcccc ttcatccctg aaccatggac gtcgccgcca tcctcctcac
    27361 catcgccggc ctctgcctct tcgagaccat ctcctccatc gacaacgcca tcatcaacgc
    27421 cgaggtcctc tccaccatgg gtgaacgtgc ccggcgctgg ttcctcgtct acgggctcat
    27481 catcgcggtc ttcctcatcc gggggatcct cccctgggcc atcatctggt ttaccacccc
    27541 ctccctcggg cccgtgggtg ccctcaccgc ggccttctcg gacgatcccc tggtcctcga
    27601 cgctatcgag gcctcggccc ccatcctcct catgggcggt ggcaccttcc tcgtcttcct
    27661 cttcttccac tggctcttcc ttgaggagaa gcacttcggc ctccggggag agcggtacat
    27721 ccagaagaag ggggtctggt tctttgccgt cgtctccgtc ctcctcaccc tcatcgtctg
    27781 gttcgccctc ggggtgagcc ccctcgtcgc cttcggcgcg gtggtgggct ccaccgcgtt
    27841 tttcatcgtc cacggcttcc ggcagaacgc cgagttccag gagaagagcc tcatggaccg
    27901 gtcgatgtcg gatatctcca agatcttcta cctcgaggtc atcgacgcca ccttctccat
    27961 cgacggggtc ctcggggcct tcgccttcac cctctcggtc cccctcatca tcctgggaaa
    28021 cggcctcggg gccttcgtgg tccgggagat caccatcggg aacatcgacc ggatcaagcg
    28081 gtacatctac ctgaagaacg gggccatgta ctccatcctc gccctgggga gcatcatgat
    28141 cctggacagc ttcggagtac atatcccgag ctacctctcc cccctcatca ccttcgggat
    28201 cgtggggtac ttcttctacc ggtccgtcct ggtcgcccgg gcccttgagg ggtcacccgg
    28261 ggattgacgg cgtcaaaagg gatttcattc ccgggaatga gatccctccc atgcacctgc
    28321 gggttccctt tctcgtcatc accctcctcc tctccctcgc acttcttacc ggctgcagca
    28381 gtcccccggc atcccagtca cccgggaccc ccgtgacgac tcctccgccg tccaccgagc
    28441 ccccggtgac caccgcgacg gtaatcccga cgacggcccc cccggaaccc tacccgggag
    28501 ctctctccat cggcacaccc taccggtacg ggagggagga catcgccatg gaggtgacgg
    28561 tgtacagggc gattaccatg gacgggtatg aatggtggtc cacggcctgg gggaagtact
    28621 ggaacacgac cccggatgag ggggaccact tcctcttcgc ttttgttcgg ctgatcgacc
    28681 ggggaaccgc acgggcccga ctgccctccc ggtcgatgtt cgtgctccac ggtggcgata
    28741 cctcctatgt ccagaacgtg gaccgggatc ccagcctctg gatcaagggg gtcggggtga
    28801 ggcagtacga cttctcctac gacacatcgg cgggctggat cgatcccggc gaatcgaaca
    28861 aggtcgaggg gttcctcctc tttgaggtac cggcgaccct ggaaccgggt gacgcccacc
    28921 tggaggtcac cttctcgagc caggcggacg ccgtctggaa gctcggctag aacacccctg
    28981 atccagggcg gtgaggatct cctccggggt tcccaccacc tccgccgact gccggatcat
    29041 gaggttcacc tttttcccgg cccacggcct ccgccggtcc gtggagaggg cgatgaccgg
    29101 gatgcccttt gccgctgcga accccatctc ccacgccgtc cccgaatcgg tgtcgacccc
    29161 gtcgcagatc gcgaccacgc aggaggcccc ctccagcgcc ttcctgtggc tcctgaagat
    29221 ggcctggtcc cgttcaggac cggcgaggcc ttctcccgtc tcctggggaa ggtagacccg
    29281 gcacccgtgg ctctcgagga ggtccctgag cacgaggtta taggcccgct cggcatccga
    29341 gaagaggggg gcggcgaggt agacttttga tcgttgtttc tctcgcttct ccatgatggt
    29401 gagatcggtg tgggaggata aaagacgagg aggtccgcca gggacgatga atcccggctc
    29461 atcggggacc atcacagccg gtttggtaca tcaggggtct tcgtcaggct cttgagggcc
    29521 ccccgaggcg caaaatattc ggcgaacgtt ccataacgct cggctttcca tgaaagctat
    29581 catgaccgtc gccgtccaaa cataccggga ccatgatccc tctcctcgcc taccgggaca
    29641 acacctgcga cccga
//
LOCUS       DATGDI010000082         4410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_269030, whole genome shotgun sequence.
ACCESSION   DATGDI010000082 DATGDI010000000
VERSION     DATGDI010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269030"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VKO45_03060"
     CDS             <1..494
                     /locus_tag="VKO45_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMA04884.1"
                     /translation="MEQIPDIQVVTMAQVQGTILSLVGSARTLILSVIVVAIIISGSG
                     IMNTLLMSVNERTREFGMMKAIGASGYDIAALVLTETILLTTAGGVTGVAFALAGSSA
                     IEAFVRGSIPYAPAGQFIAADPWLIAGCLGFSILLGMLCGLYPAVKSSRLTPMEAIRG
                     EFE"
     gene            491..1168
                     /locus_tag="VKO45_03065"
     CDS             491..1168
                     /locus_tag="VKO45_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA04885.1"
                     /translation="MSLIEARGLTKVYRRGSEEVHALRGVELNIEEGEYLAIVGPSGS
                     GKTALLNLLGCLDRPSSGSIHLDGTETGRLNEAARARLRREKIGFVFQQFLLIPTLSA
                     RENVELPLLFARKKRDREILDGILERVGLTGRGDHLPHQLSGGEMQRVAIGRALVNNP
                     RILLADEPTGNLDSATAARIFGLFDELVAKGLTLVVVTHNRELADQAHRVIRLSDGNI
                     LGEQGNS"
     gene            1340..2332
                     /locus_tag="VKO45_03070"
     CDS             1340..2332
                     /locus_tag="VKO45_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA04886.1"
                     /translation="MRGMTAEEKAALVSVGSAAIDESLLAGGMRTTATAGPGAGGSSF
                     FLRSGDRRVRLSINPNSPQRVIPEGDHVAVVRDGKVIARGTLERPLCHCPREAYVTIS
                     ERCVFNCKFCPVPLLNGEVKDTTTIVAMVEEAEKTGELEAISLTSGVADTPEGEVQRA
                     VKAVKALKERFNFPIGVSIYPTKTSSEELYAAGADEVKYNVETMDPEIFARVCPGLSL
                     PFILESLEKAVQIFGKNHVASNFILGLGETDACVKDGVETLTAMGVIPVLRPISPHPL
                     RKGEITVERPSAERLLRLARLHREALERHGLDPRKAHTMCLPCTGCDLTPCRDV"
     gene            complement(2380..3291)
                     /gene="nadA"
                     /locus_tag="VKO45_03075"
     CDS             complement(2380..3291)
                     /gene="nadA"
                     /locus_tag="VKO45_03075"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HMA04887.1"
                     /translation="MENEKELIERIQGLREEREAIILAHNYQIGPVQDIADLVGDSLE
                     LSRAAASMEGKVIVFCGVDFMAETAAILSPGKKVLLPAIDACCPMAAMVTGGELRVLR
                     ERYSDAAVVAYVNTTAEVKAESDICCTSANAVKVVESLDQERVIFVPDRNLALYVARF
                     TKKEILPWEGYCIVHDRYTPADVMAARSQHPGAELIVHPECRPEVIDLADGVFSTSGI
                     IRHACTGHGKEFIIGTEVGILHRLGKECPEKLCYPLSEKAVCVNMKRTTLRKVYRSLE
                     RMAPQVTIPDEIAGRARRAIERMLAIQ"
     gene            complement(3441..>4410)
                     /locus_tag="VKO45_03080"
     CDS             complement(3441..>4410)
                     /locus_tag="VKO45_03080"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HMA04888.1"
                     /translation="GAESGELVFTRNTTEAINMVALGLPWQKGDRIVTTLLEHHSNLL
                     PWMRLRDRAGIDLRLITPAPNGTFNLDEFEEAMTPETRLVAVTHASNVLGNIVMVREI
                     AAICQDHGALLLVDGAQSVPHIPIDVERLGCDFLCFSGHKMLGPTGTGALWMRQPEIE
                     PLFLGGGAVEQVTTSSYTLAQGAARYEGGTPNIAGAIGLSRAVEYLEALGMEAVKKHE
                     EALTQKLFDGLSGIDGVKVFGPGTKAERIGVVSFTVEGLHPHDVAIILDSEADIMVRS
                     GHHCCMPLMEHLGTPDGTVRASLHCYNNEEDVDRFLETVGEIARCL"
BASE COUNT          807 a         1380 c         1392 g          831 t
ORIGIN      
        1 aaatggagca gatccccgac atccaggtgg tgaccatggc ccaggtgcag gggaccatcc
       61 tttccctggt gggatcggcg cggaccctca tcctctcggt gatcgtcgtc gccataatca
      121 tcagcggctc agggatcatg aacaccctcc tcatgtcggt gaacgagcgg acccgggaat
      181 tcgggatgat gaaggctatc ggcgcctctg gatatgacat cgctgcgctc gtcctcacgg
      241 agacgatcct cctcaccacc gcgggcggag tgacgggagt cgccttcgcc ctcgccggga
      301 gcagcgccat cgaggccttc gttaggggga gcatccccta tgccccggca gggcagttca
      361 ttgcggccga cccctggctc atcgcgggat gcctcggctt ctcgattctc ctcggcatgc
      421 tctgcgggct ctaccccgcg gtgaagtcct cgcgcctcac cccgatggag gcgatccggg
      481 gggagttcga atgagcctca tcgaggcccg gggcctcacg aaggtctacc ggcgcgggag
      541 cgaggaggtc catgccctcc ggggggtgga actgaacatc gaagaggggg agtacctggc
      601 cattgtgggc ccctccggtt cggggaaaac ggcgctcctg aacctcctgg ggtgtctgga
      661 tcgcccgagc tccgggagca tacacctgga cggtaccgaa acgggaaggc tcaacgaggc
      721 tgcaagggca cggcttcgcc gcgagaagat cgggttcgtc tttcagcagt tcctcctcat
      781 ccccaccctc tcggcccgcg agaacgtgga actcccactc ctctttgccc ggaagaagag
      841 agaccgggag atcctcgacg ggatcctcga gcgtgtcggg ctcaccggcc gcggggatca
      901 cctcccccac cagctctccg gcggtgagat gcaacgggtg gccatcggga gggcgctcgt
      961 caacaacccg cggatcctcc tcgcggacga gccgacaggg aacctcgact cggccacggc
     1021 ggcgaggatc tttggcctct tcgatgagct ggtggcaaag gggcttaccc tggtcgtcgt
     1081 tacccataac cgggaactcg cagaccaggc gcaccgggtg atccggctca gcgacgggaa
     1141 catcttgggg gaacagggga actcctgatc tcctttcggg aggatgcttt ctgacattcc
     1201 agagttgcct caagaccaag gtataaggta tgcaacgatt atattcacaa ttgtgaacca
     1261 ttgaaacagg gtatcctcca gatgacgggg aaaccaggtg agggtcggag gttaggcaat
     1321 gtcggtgaag ggaaagaaaa tgaggggaat gacggcagag gagaaggccg ccctcgtgag
     1381 cgtcgggtcg gcggccatcg acgagtccct cctcgcgggg gggatgagga ccacggcgac
     1441 ggcggggccg ggtgcaggcg ggtcatcctt cttcctcagg tcgggggacc ggcgggtcag
     1501 gctctccatc aaccccaatt ctccccagag ggtcattccc gagggcgacc atgtcgcggt
     1561 tgtcagggac ggtaaagtga tagcccgggg gacgctggag cgacctctct gccactgtcc
     1621 gagggaggcc tatgtcacca tcagcgagcg gtgcgtcttt aactgcaagt tctgcccggt
     1681 cccgctcctg aacggcgagg taaaggacac cacgaccatc gttgccatgg tcgaagaggc
     1741 ggagaagacc ggcgaacttg aggccatctc cctcacaagc ggggtcgcgg ataccccgga
     1801 gggggaggtt caacgggcgg ttaaggccgt gaaggcactc aaggaacgat tcaactttcc
     1861 catcggggtc tccatctacc cgacgaagac ctcctcggag gagctttacg ctgccggtgc
     1921 cgacgaggtc aagtacaacg tcgagaccat ggacccggag atctttgccc gggtctgccc
     1981 cggcctctcc ctccccttca tcctggagtc gctggaaaag gcggtccaga tcttcgggaa
     2041 gaaccacgtt gcctcgaact tcatcctcgg cctcggggag accgacgcct gcgtgaagga
     2101 cggggtggag accctcacgg cgatgggcgt gattccggta ttgcgcccca tctcgcccca
     2161 ccccctgcgg aagggcgaga tcaccgtcga gcgcccctcg gccgagcggc tcctccggct
     2221 ggcgcggttg caccgggagg ccctcgaacg gcacggtctg gacccgagga aggcccacac
     2281 gatgtgcctc ccctgcacgg gctgcgacct gaccccctgc cgggacgtgt aaaagccggg
     2341 gattagatcg aataaggatc gcattcgaaa cgatccctct cactggatgg cgagcatccg
     2401 ttcaatggct ctccgggccc ggccggcgat ctcatcgggg atggtgacct gcggcgccat
     2461 ccgctcgagg gaacgataca ccttccgcag cgtcgttctc ttcatgttga cacagaccgc
     2521 cttctctgag agggggtagc aaagcttctc ggggcattcc tttccaagcc ggtgaaggat
     2581 cccgacctcg gtcccgatga taaactcctt cccatgacct gtgcacgcat gccggatgat
     2641 ccccgatgtc gagaagactc cgtcggcaag atcgatgacc tccggccggc actcggggtg
     2701 tacgatcagc tctgccccgg ggtgctggct ccgtgccgcc atgacatcgg cgggcgtgta
     2761 tcggtcatgg acgatgcaat aaccctccca gggaaggatc tccttcttcg tgaaccgtgc
     2821 cacgtagagg gcgaggttcc ggtccgggac gaagatcacc cgttcctgat cgagggactc
     2881 caccaccttc acggcatttg cggaggtgca gcagatatcg ctctctgctt tcacctccgc
     2941 cgtggtgttc acgtaggcga ccaccgctgc atcggaatac cgctccctga gcacccgcag
     3001 ctccccgccg gtgaccatcg cggccatggg gcagcaggcg tcgatggcgg ggaggaggac
     3061 cttcttcccg ggtgaaagga tggccgcggt ttctgccatg aagtccacac cgcagaagac
     3121 gatcaccttc ccctccatgg atgcggccgc tcgggagagc tccagggagt cccccacgag
     3181 gtcggcgatg tcctgaaccg gtccgatctg gtagttgtgg gcgagaatga tggcctcccg
     3241 ttcctctctg agaccctgga tcctttcgat gagttctttt tcgttttcca tgatcaccct
     3301 cttcccccga cccgagaata gcggccctca cggcctgacg ggatcgcctc cacctcctgt
     3361 tctttcgtat ttgataaggg ttgctctgga atgcatgggt tcaccgccga tcccggttac
     3421 gacaggccag gggtagtcac tcacaaacat ctcgcaatct ccccgaccgt ctccaggaaa
     3481 cggtccacgt cctcctcgtt attgtagcag tggagggatg cccgcaccgt cccatcggga
     3541 gttccgagat gttccatgag gggcatgcag cagtggtgtc ctgatctcac catgatatcg
     3601 gcctcgctgt cgaggatgat ggccacgtca tgggggtgaa gaccttccac ggtgaaggat
     3661 accaccccga tccgctctgc cttcgttccg gggccgaaaa ccttcacgcc gtcaatacct
     3721 gaaagcccgt cgaacagctt ttgcgtgagg gcctcctcgt gtttcttcac tgcttccatt
     3781 ccgagggcct caaggtactc gacagcccgg gagagaccga tggccccggc gatgttcggg
     3841 gtccctccct cgtaccgggc tgctccctgc gcgagggtat agctcgaggt ggtgacctgt
     3901 tcaacagctc ctccaccgag gaacagtggt tcgatctccg gttgcctcat ccagagggcc
     3961 cctgtcccgg tcggaccaag cattttgtgc cccgagaagc agaggaagtc gcagcccagg
     4021 cgttccacgt caatggggat atgggggacc gactgggcgc catccacgag aaggagggca
     4081 ccgtggtcct ggcagatggc ggcgatctcg cggaccatga cgatgttccc cagcacgttc
     4141 gatgcatggg tgaccgcaac gagccgggtc tccggggtca tggcttcttc gaactcatcc
     4201 aggttgaagg taccgttcgg cgccggggtg atgagccgga ggtcgatccc ggcccggtcc
     4261 ctgaggcgca tccaggggag gaggttgctg tggtgttcga ggagtgtggt gacaatccgg
     4321 tccccttttt gccacgggag accgagggct accatgttga tcgcctcggt ggtattccgg
     4381 gtgaagacga gctcgccgct ctccgcgccg
//
LOCUS       DATGDI010000083        12052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_414687, whole genome shotgun sequence.
ACCESSION   DATGDI010000083 DATGDI010000000
VERSION     DATGDI010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 12052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12052
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414687"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..102)
                     /locus_tag="VKO45_03085"
     CDS             complement(<1..102)
                     /locus_tag="VKO45_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HMA04889.1"
                     /translation="MCIAIPAEVVELKGNNVGVVDYGDLRQEVQLDLV"
     gene            complement(130..214)
                     /locus_tag="VKO45_03090"
     tRNA            complement(130..214)
                     /locus_tag="VKO45_03090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(178..180),aa:Ser,seq:tga)
     gene            complement(273..1082)
                     /locus_tag="VKO45_03095"
     CDS             complement(273..1082)
                     /locus_tag="VKO45_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04890.1"
                     /translation="MKRIAWILLVGVFLVGAAQAAYVELNAPSEINVGQTLDVTGTSI
                     GLSAGFSTDLIFYRYSGSKIEIARTRIVVQDGGGFSARFSTDGLSAGTYLLELVDPMT
                     GGNAQFGGQVKNIIYVTLINRQGNITITSPYSQSFTGLLSIRGSIRDARNFGTQLQVD
                     HNGRTIFGPLYVATLNDAFSTDIPITEGGIYTAYFSDSHGNIGSAQFTVSQPVHTTVP
                     TTIATTIKTSVASTTPPLTWTPPPTTQKSPLPALLALIALAMALPVIARRR"
     gene            1110..2093
                     /locus_tag="VKO45_03100"
     CDS             1110..2093
                     /locus_tag="VKO45_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine biosynthesis radical SAM protein
                     RaSEA"
                     /protein_id="HMA04891.1"
                     /translation="MVSCQTERPVAAWIDRERRGTGVVPALTLILKSGGCGWNRCLMC
                     GYRHFRYPREEVSLPDRIRAQLGWVAERFAGEDYGIVKIFTSGSFLDPGEVPPEARRE
                     ILRAFRGREIIVETRPEFVTGETVGECLQELDSGTGPVSFTVAIGLETSDDAIREKSI
                     MKGFSFADFVDASRTAHQAGAGVKAYLLQKPPYLTEKEAIGDVVRSVHDAASHADAIS
                     LNPCTVQRGTEVERLWRARSYRPPYLWSVILALSRAGVPVTCDPVGGGTPRGPHNCPS
                     CDDELVRGIRDYSLSGDRTLLDALLGMDCGCKKEWEFILGAERPWCMPLTG"
     gene            complement(2083..3321)
                     /locus_tag="VKO45_03105"
     CDS             complement(2083..3321)
                     /locus_tag="VKO45_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HMA04892.1"
                     /translation="MEASREIELEGHIIDSGIMTQVFDRIMDMEGNFEILEFDVGKRK
                     TDPSYAKLRVKARDETKLSLILSELHRFGARPVEIEDCHLVAAEGNRIAPRDFYSTTN
                     HPTFIRHEGEWIPVDEMKMDSLVVFDAKTGRAVCKTLSKLLAGELVVVGEDGVKVVPP
                     ARPRKQSLFEFMHGTVSPERPSEAIIAQIAKEMIELKRKGGKIILVGGPAIIHTGASP
                     ALARIIRMGYVDALLAGNALAAHDIEYSLVGTSLGMDLSTGKPVIGGHCHHLQAISEV
                     IRAGSIRDAVEKGVVKSGIMYECVKKGIPYVLAGSIRDDGPLPDVITDVVAAQDAMRE
                     ELKGCGMVLMIATLLHSIAVGNCLPSSVKTVCVDINPTSVTKLMDRGTQQVIGVVSDA
                     GTFLPLLAKQLEIQSGGVSR"
     gene            complement(3473..4606)
                     /locus_tag="VKO45_03110"
     CDS             complement(3473..4606)
                     /locus_tag="VKO45_03110"
                     /inference="COORDINATES: protein motif:HMM:NF044575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HMA04893.1"
                     /translation="MLALILAGGEGSRLGLGEKPLVDICGKPMVAWVIDAFTGAGLEV
                     AVVASSRTPMTLNYLRAQGIPFYRAQGRGYVEDIVEAVTTLEVSAPLFTSVADIPCLR
                     PDHVKKIREAYLSQEKPALSSWIPRDLCPVGGCRTGYTEMVDGVPSAPAGVNVLLGER
                     IREPQEEHRLLLHDPALAWNVNTPEELEAVRRVVCGGRFRETKLRETDLEQPAIRRGA
                     TRMRKRKIAVTPLDGPIEELVGKTDQKTLAIWAADCASRVLPHFEEKYPGDTRPRNAI
                     EALREWVSTGMFRMADVRRTALAAHAAARDVQDDDAARSAARAAGQAMATAHVPRHAL
                     AAAMYAATAVRDAADPAESDNATIREREWQYRHLLGLMETDIP"
     gene            complement(4594..5592)
                     /locus_tag="VKO45_03115"
     CDS             complement(4594..5592)
                     /locus_tag="VKO45_03115"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HMA04894.1"
                     /translation="MKKELPERVVHGGTFLWHQGRSGIPLLDFSANLNPFPPDISWSP
                     DPSVLASYPDDRYEALRETIGRTFHRSPEEVAVGNGSMEIIRVFCQAAFSPGDHFLAD
                     QPTFGEYDLSARLAGALPVSEPGRARVRFLCNPNNPTGALLPADRVRGILPQVRAGPG
                     ILFLDEAFIELSDPGQSLIRERDPALFLLRSLTKAFAVPGLRFGYGFGDPDLVARMEV
                     IRSPWSVNAFAEDFALLAFRYYDRLEESRQRITRERTWLEEKLRVLGLAPSPSKANFL
                     LVPLKMQASSLTARLLEKGILVRDCTSFGLPDAIRIAVRKREENERLIEALTACLR"
     gene            5905..6081
                     /locus_tag="VKO45_03120"
     CDS             5905..6081
                     /locus_tag="VKO45_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HMA04895.1"
                     /translation="MERVTIRLPRQQVDMLEKLVSAGEFPTVSEAVRYAVREFVEKHA
                     SRVIKESDQVSFNV"
     gene            6093..7232
                     /gene="ftsZ"
                     /locus_tag="VKO45_03125"
     CDS             6093..7232
                     /gene="ftsZ"
                     /locus_tag="VKO45_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMA04896.1"
                     /translation="MQSLIDEAVKNAELEKTDTKTTEADDFIGAPRILVVGCGGAGNN
                     TVNRLYHMGIQGAETIAINTDKQHLDMIQAGKRVLIGKSLTKGLGAGGYPDIGKRAAE
                     MARPTLESLMESADLVFVTAGMGGGTGTGSAPVVAQIAKDQGAIVVGMVSYPFQVEKA
                     RLLRAEEGLEGLSKAADSVIVLDNNRLLSYVPNLPIGQAFSVMDQLIAETVKGISETI
                     TEPSLIQLDYADVRAIMSKGGVAVMLVGESKQQNKAESVVHECLNHPLLDIDYRGATG
                     SLIHITGGNDLTLADAEEIASSLTYELDPHADVIWGARIRNEFEGKVRVMAIMTGVRS
                     AQVLGPYAETAAKVTHTAVPQKAAAATRETTTRGDRKSGGLIDFI"
     gene            7554..8414
                     /locus_tag="VKO45_03130"
     CDS             7554..8414
                     /locus_tag="VKO45_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HMA04897.1"
                     /translation="MMNDLIEKRKKTLAESEQHKTRRNELNAQASKFAGERNALNTQT
                     RGFVEEAQQNKDLRDKYNTEVQGLKEQRNDLNRKANELFEEIEAYKKEHGTLRNRGLK
                     ELQKQVEFMEFRQQTEVFTTDKERELIEKIKQMKESIKEQEAELEQNKEIRSKLTQAR
                     DFRKEASDLHAKVTEMADLAQKHHDAMVECYKKADKSRESADAVHRQFVEAQEAADAE
                     HKFFIACQKELRDFDKVIGGLRKKTAKAKVTKEQKEVRKEAERIFNLFRGGEKLTTDD
                     ILLLQRSKLI"
     gene            8495..9619
                     /locus_tag="VKO45_03135"
     CDS             8495..9619
                     /locus_tag="VKO45_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
                     /protein_id="HMA04898.1"
                     /translation="MPEKKTLVLCVDRDDDIGYKAKIESPVIGREACLGAATALALAD
                     PEDSDVNAIFEGIRIYDDLARRGEGVAIAVLAGDHLDMIQGDRKMAADLEQVIRLTGA
                     GSCILVSDGAEDEYVLPIVQSRIPVASVRRVIVSQMPNLESSYYTIKKLIDDPKVSRV
                     FLAPIGLAMLLFAVATLLGYPEGATVIVLGVVGVYILFRVFGVDSAFGAGFSSLQSSL
                     EKGRFSFVTSISAIVLAVIGIVLGSASVFENYTSEFTMGIFLYVISFLYGSLGWFTAS
                     VLIITAGKVIDVYMNDPAGLARTIVLPFFAGSIGLIAYGASVYTLSVSNLLTFPYTPS
                     QGSQFITLAVVGGLLVAIIGVYAQSLVGRWTREKTGTSAE"
     gene            complement(9627..10079)
                     /locus_tag="VKO45_03140"
     CDS             complement(9627..10079)
                     /locus_tag="VKO45_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04899.1"
                     /translation="MRSSLPVLLVLLLVGIAYPALAAEGNRVGYVTFEEARVQLLDGN
                     ARVDVDYSVDPGMGLIILLFGSGDLQKKIERSLNFPSARPEEVGLTHAVFLVDHAAED
                     YGDHAYWFPGHRFGVTFPAVEVRAPGYSLFFPDAGSIPKSFGYFSAGS"
     gene            complement(10290..11918)
                     /locus_tag="VKO45_03145"
     CDS             complement(10290..11918)
                     /locus_tag="VKO45_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HMA04900.1"
                     /translation="MVSRFRRYGQIADVLVKYGFGIALDELFPGVRKWKFFEKHFPEQ
                     RPVYERMRLAIEELGPTFIKFGQIASTRTEILPPELIRELHQLQDRVAPAPFSRVKPV
                     FEEYCGCTEDVFEQIDEEPVGSASIALVYRGVLKDGTKVALKVQRPGIAEVIEKDLVI
                     LQALAKRVEAVFPDLRVYNPTGIVEDFSRQIRKELDFVRDGKNAERLGQNMRDVQGVR
                     TPKIYWKYSGARLLVMEYVEGVRIDDANGIRGFGISPREIGTRGFYAYMKQIFEDGFF
                     HGDPHPGNLIVTPKGDLYFLDFGIMGIIHPGKRLIFTNLLASILQNDVETLLRSLERL
                     GVRVRAEDMDNLRDDLYIVLMDYSEYRFKEVNISQVIQELSDVMRRYQMQVPMNLMLM
                     LKVIMMVADIGKSLDPQFNFSSYVEPYMGDLAEKEYSGRKVLSRARESARTAIEGIAE
                     LPLNMNSVLKRLSTGSLRVEIAEEDLRRVQHISDRASDRILVGLITAALVVGSSIVIF
                     ASGTSITGPLLFLAYLGYAVAVIIGFIALYYSLK"
BASE COUNT         2316 a         3738 c         3811 g         2187 t
ORIGIN      
        1 cacgaggtcg agctggacct cctgcctgag gtcaccgtag tccaccaccc cgacgttatt
       61 tcccttcagc tccacgacct cggcgggaat cgcgatgcac atgactctct tcctcctctc
      121 tccgaaaagc gccggggagg agatttgaac tcccgaggtg ctgggcacca gcggctttca
      181 aggccgccgc ctttccggac tagactaccc cggctccctc agagagttgg ttccggcgcg
      241 taaaaagctt tcaaggcccc aaaaaattgg gattacctcc gcctggcgat gaccgggagg
      301 gccatggcga gggcgatgag ggccagaagg gcggggagcg ggctcttctg ggtggtcggg
      361 ggaggtgtcc atgtcaacgg aggtgtggtg gatgccacgg atgtttttat cgtggtggcg
      421 atggtggtgg ggaccgtggt atggaccggc tgcgagacgg tgaactgggc cgatccgatg
      481 ttaccatggg aatcagagaa atatgcggtg tagatccccc cttccgtgat gggtatgtcc
      541 gtcgagaatg catcgttgag ggtggcgacg tataagggcc cgaagatggt cctcccgttg
      601 tggtctacct ggagctgggt cccgaaattt cgtgcatccc ggatagaccc tcggatggac
      661 agaaggccgg tgaaagactg ggaatacggg gaggtaatgg taatattccc ctggcggttg
      721 atgagggtga cgtagatgat attcttcacc tggcctccga actgggcatt ccctccggtc
      781 atgggatcga caagctcgag gaggtaggtc cctgccgaaa gaccgtcggt gctgaacctc
      841 gcggagaacc ctcccccgtc ctggaccacg atccgggtcc gggcgatctc tattttagac
      901 ccgctgtacc ggtagaagat aagatcggtg gagaaccccg cagagagccc gatggacgtc
      961 ccggtcacgt ccagggtctg gccgacgttg atctcgctgg gagcgttcag ctcgacatag
     1021 gctgcctgtg cggcaccgac gaggaacacc cccacgagca gtatccaggc aatccttttc
     1081 ataggtacac atccaactac cgggaataaa tggtttcttg tcagaccgag cggccggttg
     1141 cggcctggat cgaccgggaa cgtcggggaa ccggggtggt ccccgccctc accctcatcc
     1201 tgaagagcgg cgggtgcggg tggaaccggt gccttatgtg cgggtaccgg cacttcaggt
     1261 acccccggga agaggtatcc ctcccggatc ggattcgggc ccagctcggg tgggtcgccg
     1321 agcgattcgc aggtgaggac tacgggatcg tgaagatttt cacttcgggc tccttcctcg
     1381 acccgggaga ggtgccgccg gaggcgagaa gggagatcct ccgtgccttc aggggcaggg
     1441 agatcatcgt cgagacccgg ccggagtttg tcaccgggga gacggtgggg gaatgcctgc
     1501 aggagctgga ttccgggacc ggccccgtct ccttcacagt tgccatcggg cttgagacct
     1561 cagatgacgc gatccgcgag aagagcatca tgaaggggtt cagcttcgcc gatttcgtgg
     1621 atgcatcgcg caccgcccac caggcaggcg caggggtgaa ggcctacctc ctccagaaac
     1681 ccccgtacct gaccgaaaag gaggccatcg gagacgtcgt ccggtcggtt catgatgccg
     1741 catcccatgc ggatgccatc tccctcaatc cctgcaccgt ccagcggggt acggaggtgg
     1801 agcggctctg gcgggcccgg tcctaccgcc ccccttatct ctggtcggtc atcctcgccc
     1861 tctcccgggc gggcgtaccg gtcacctgcg atccggtcgg cggcgggacg ccccgcgggc
     1921 cccacaactg cccgtcctgc gacgatgagc tcgtcagggg aatccgggat tacagccttt
     1981 cgggggaccg gacactcctc gacgccctcc tcggcatgga ctgcgggtgt aaaaaggagt
     2041 gggagttcat cctcggggcc gagcgcccct ggtgcatgcc ccttaccggc tgaccccgcc
     2101 cgactggatc tcgagctgct tcgcgaggag ggggaggaag gtaccggcgt cggagacgac
     2161 cccgatgacc tgctgggtcc cgcggtccat gagcttcgtg accgaggtcg ggttgatgtc
     2221 cacgcagacc gtctttaccg aggagggaag gcagttcccc acggcgatgg agtggaggag
     2281 ggtcgcgatc atgagaacca tgccgcagcc cttcagctcc tcccgcatgg cgtcctgggc
     2341 cgcgaccacg tccgtgatca cgtcggggag cgggccgtcg tcccggatgg accccgcgag
     2401 gacgtagggt atccccttct tcacgcactc gtacatgatc ccgctcttca cgaccccctt
     2461 ctccaccgcg tcccggatgg accccgcccg gatcacctcg ctgatggcct ggaggtggtg
     2521 gcagtgaccg ccgatgaccg gttttcccgt ggaaaggtcc atcccgaggg acgtccctac
     2581 caggctgtac tcgatgtcat gggccgcgag ggcatttccc gctaagaggg cgtcgacgta
     2641 gcccatgcgg atgatcctcg cgagggccgg ggaggccccg gtgtggatga tggccgggcc
     2701 ccccacgagg atgatctttc cgcccttccg cttgagctcg atcatctcct tcgcgatctg
     2761 ggcgatgatg gcctcgctcg gccgctccgg agagacggtg ccgtgcatga actcaaagag
     2821 ggactgcttc cgggggcggg ccgggggcac cacctttacc ccgtcctccc ccaccacgac
     2881 cagttccccg gcgaggagct tggagagggt cttgcagacc gcccgcccgg tctttgcgtc
     2941 gaagaccacc aggctgtcca tcttcatctc gtccacgggg atccattccc cttcgtggcg
     3001 gatgaaggtc gggtggttcg ttgtcgagta gaagtcccgg ggggcgatgc ggttcccctc
     3061 ggcggcgacc aggtggcagt cctcgatctc cacgggccgg gccccgaacc ggtggagctc
     3121 ggagaggatg agggagagtt tggtctcgtc ccgggccttg acccggagct tcgcgtacga
     3181 tggatccgtc ttccgcttcc ccacgtcgaa ctccaggatc tcgaagttcc cctccatgtc
     3241 catgatccgg tcaaagacct gggtcatgat ccccgagtcg atgatatggc cctcgagctc
     3301 gatctcccgg gaggcttcca tgcctagacg tcggtttgga tggttaatat ggttaatccc
     3361 ggaggatcct ctcccggaaa ccgaggattc tcaaccgtgg aatggatcct tccgagaatc
     3421 gacagatatc gaagatcacc ggtcccgtga cagaggacac cgcggcgggc cattagggga
     3481 tatctgtttc catcagtccg agtaagtgac ggtattgcca ctcacgttcc ctgatcgtgg
     3541 cattatcgga ttcagcggga tcggcagcat ccctgacagc ggttgcggcg tacatcgcag
     3601 cggcgagagc gtgccggggg acatgagccg ttgccatcgc ctggcctgcg gcacgggcag
     3661 cagaacgggc tgcatcatcg tcctgcacgt cacgggcggc tgcatgagcc gcaagggcag
     3721 ttctccggac atcggccatc ctgaacatgc cggtacttac ccactccctg agcgcttcta
     3781 tcgcgtttcg tggacgggtg tctcccgggt atttctcctc aaagtggggc aggacacggg
     3841 aagcacagtc ggcagcccag attgccaggg ttttctggtc agtcttcccc accagctctt
     3901 cgatgggacc gtccagcggt gtgaccgcga tcttccgctt cctcattcgg gtcgcacccc
     3961 gcctgatggc gggttgctcc aggtcggttt ctcgcaactt cgtctctcga aacctccctc
     4021 cgcacaccac cctccgcacc gcctccagct cctccggggt gttcacgttc catgcaaggg
     4081 cggggtcatg gaggaggagc cggtgctctt cctggggttc ccggatcctc tcaccgagga
     4141 ggacattcac cccggcaggg gccgagggaa ccccgtcgac catctcggta taccctgtcc
     4201 ggcacccgcc cacagggcag aggtcccggg ggatccagct ggagagggcc ggcttctcct
     4261 gggagaggta ggcctccctt attttcttca catgatccgg ccggaggcag ggaatatcgg
     4321 cgaccgaggt gaagaggggg gccgagacct cgagggtggt gaccgcctcc acgatgtcct
     4381 ccacgtaccc cctgccctgc gcacggtaga atgggatccc ctgagcccgg aggtagttca
     4441 gggtcatggg ggttcgggag gatgccacca ccgcaacctc gagcccggca cccgtgaagg
     4501 catcgataac ccacgcaacc atgggctttc cgcagatgtc gacgaggggc ttctctccca
     4561 gcccgagccg ggatccctcc ccacccgcga ggatcagcgc aagcatgcgg tgagggcctc
     4621 gatgagccgc tcgttctcct cccttttccg gaccgcgatc cggatggcgt ccgggaggcc
     4681 gaaggaggtg cagtcccgga cgaggatacc cttttcgagg agccgggcgg tgagggagga
     4741 ggcctgcatc ttcaggggca cgaggaggaa gttcgctttc gagggagagg gggcaagtcc
     4801 caggacccgg agcttctcct ccagccatgt cctttctctc gtgatcctct gccgtgattc
     4861 ctccagccgg tcatagtacc tgaaggcgag gagggcgaaa tcctcggcaa aggcgttcac
     4921 cgaccagggg gaccggatca cctccatccg ggcgacgaga tcgggatccc cgaacccgta
     4981 cccgaaccgg aggcccggga ccgcgaaggc cttcgtcagg gaccggagga ggaagagggc
     5041 cgggtccctc tcccggatga ggctctggcc ggggtccgag agctcgataa aggcctcgtc
     5101 caggaacagg atgccgggcc cggcgcggac ctgcggcagg atacctctca cgcgatccgc
     5161 cgggaggagg gcccccgtcg ggttgttggg attgcagagg aaccggaccc gcgcgcggcc
     5221 cggttcagaa accgggaggg caccggccag ccgggccgag agatcgtact ccccgaaggt
     5281 gggctggtcg gccaggaagt ggtcgcccgg ggagaaggcc gcctggcaga agacccggat
     5341 gatctccatg gagccgttcc ccaccgctac ctcctccggg gagcggtgaa aagtcctccc
     5401 gatggtctcc cggagggcct cgtaccggtc atcagggtag gaggcgagga cagaggggtc
     5461 tggactccag gagatatcag gcgggaaggg gttcaggttc gccgagaagt ccaggagggg
     5521 aatccccgac ctcccctggt gccagaggaa agtgcctcca tggacgaccc tctccggtaa
     5581 ctcctttttc acggtctcct ccccaatcgg gaagacaacc ttcccttatg ggaaggcaac
     5641 ttccctaaac gggaagactc ccttccctga tgcgaaggaa acttccctaa acaggaagtg
     5701 ccttctcctg atggaacggc cttccctaac cgggaagtat ttgaaaatac taacttaaaa
     5761 gaatatggaa ttcggcctgg ttcccggaca gaaggtttaa atacttacaa acaaatcaat
     5821 ttttatccgc ttgacggaca attgtagcac agatgtctga cacaaagcag acataaagca
     5881 gccaagtgtc agatagtgat gaaaatggaa cgggtcacca tccgcttacc aaggcagcag
     5941 gtggacatgc tggagaagct cgtctcagcc ggcgagttcc ccaccgtctc cgaggccgtg
     6001 cgctacgcgg tcagggagtt cgtggagaag catgcgagcc gcgtcatcaa ggagagcgac
     6061 caggtttcat tcaacgtcta ggaggaaatg gtatgcagag cctgattgat gaagccgtaa
     6121 agaatgcaga actcgaaaag accgatacca agacgaccga ggccgatgac ttcatcggcg
     6181 caccccggat cctcgtggtc gggtgcggtg gcgcgggaaa caacaccgtg aaccggctct
     6241 accacatggg gatccagggc gccgagacca tcgccatcaa caccgacaag cagcacctgg
     6301 acatgatcca ggcgggaaag cgcgtcctca tcgggaagtc cctgacaaag ggcctcggtg
     6361 cgggcgggta cccggacatc gggaagcgcg ccgccgagat ggcccgcccg accctcgaga
     6421 gcctgatgga gtcggcggac ctcgtctttg tcaccgccgg tatgggtggc ggtaccggta
     6481 ccgggtcggc acccgtcgtc gcccagatcg cgaaggacca gggtgccatc gtcgttggaa
     6541 tggtctccta ccccttccag gtggagaagg cacgcctcct ccgcgccgag gagggcctcg
     6601 aggggctctc caaggcggcc gactcggtca tcgtccttga caacaaccgg ctgctctcct
     6661 atgtgccgaa cctccccatc gggcaggcct tctcggtcat ggaccagctc atcgccgaga
     6721 ccgtcaaggg gatctccgag accatcaccg agccgtccct catccagctc gactacgccg
     6781 acgtccgggc catcatgtcc aagggcggcg tcgcggtcat gctcgtgggc gagtccaagc
     6841 agcagaacaa ggccgagtcc gtggtccacg agtgcctgaa ccacccgctc ctcgatattg
     6901 actaccgggg cgcgacgggg tccctcatcc acatcaccgg cgggaacgac ctcaccctcg
     6961 cggatgccga ggagatcgcc tcgtccctca cctacgagct cgacccccac gcggatgtca
     7021 tctggggcgc ccggatccgg aacgagttcg agggcaaggt ccgggtcatg gccatcatga
     7081 ccggcgtccg gtccgcccag gtcctcgggc cctatgcaga gaccgcggca aaggtcaccc
     7141 acacagccgt cccgcagaag gcggcggccg cgacccggga gaccacgacc cgcggggacc
     7201 ggaagagcgg cggcctcatc gatttcatct aaatcccccc actctccttc ctttcttcga
     7261 aatccttaag aatcaaaaag gcattttaca tatgcagaat cctggcgtga taccgccttt
     7321 tatgcgtgtg atgccaggtc tcgcgtgcag gaaggggtcc ggcgcacgca ggaacgggag
     7381 ttgaacacca tgcaccgcca gatgaacatc ggagggacgg ccaccccgag gccgttcgtg
     7441 ccgatagcgc tcttctcctg gtatgctccc gtttcctgcc cgggcggcag gatcacgtca
     7501 ccggtccctg cgtggggtcg agaaccggta ccgactgttg aggatgaagg aagatgatga
     7561 acgatctcat cgagaagagg aaaaagaccc ttgcggaatc cgagcagcac aagacccgga
     7621 gaaacgagct gaacgcccag gcgagcaagt tcgccggcga gaggaacgcc ctgaacaccc
     7681 agacccgggg cttcgtcgag gaggcccagc agaacaagga tctccgggac aagtacaaca
     7741 ccgaggtcca gggcctcaag gagcagagga acgatctgaa caggaaggcc aacgagctct
     7801 tcgaggagat cgaggcctac aagaaggagc acgggaccct ccggaaccgg gggctgaagg
     7861 agcttcagaa gcaggtcgag ttcatggagt tccggcagca gaccgaggtc ttcaccaccg
     7921 acaaggaacg ggagctcatc gagaagatca agcagatgaa ggagtccatc aaggagcagg
     7981 aggccgagct cgagcagaac aaggagatcc ggtccaagct cacccaggcc cgggatttcc
     8041 ggaaggaggc ctccgatctc catgccaagg tgaccgagat ggcggatctc gcccagaagc
     8101 accacgacgc catggtggag tgctacaaga aggcggacaa gtcccgggag tccgccgacg
     8161 cggtccaccg gcagttcgtc gaggcccagg aggcggccga cgcggagcac aagttcttca
     8221 tcgcctgcca gaaggagctc cgtgacttcg acaaggtcat cggcggcctc cggaagaaga
     8281 cggcaaaggc caaggtcacg aaggagcaga aggaggtgag gaaggaggcc gagcggatct
     8341 tcaacctctt ccggggcggc gagaagctca ccaccgacga tatcctcctc ctccagcgct
     8401 caaagctcat ctgataccct ttttttcgcc tattttcccg aaacggctgc ccgatgctta
     8461 tataggttcg cgtcgataac gtctattgaa gagaatgccg gagaaaaaga ccctcgtcct
     8521 ctgtgtggac cgggacgatg atatcgggta caaggcaaag atcgaaagcc cggtcatcgg
     8581 gagggaggcc tgcctcgggg cggcgacggc cctcgccctc gccgacccgg aggattcgga
     8641 cgtgaatgcc atctttgagg ggatccggat ctacgacgat ctggcccgga gaggggaagg
     8701 cgtggccatc gccgtcctcg ccggggacca cctcgacatg atccaggggg accggaagat
     8761 ggccgcggac ctcgagcagg tgatccggct caccggcgcc gggtcatgca tcctcgtctc
     8821 ggacggggcc gaggacgagt acgtcctccc catcgtccag tcgaggatcc ccgtggcctc
     8881 ggtccggcgg gtgatcgtct cccagatgcc gaacctcgag tcctcctatt acaccatcaa
     8941 gaagctcatc gacgacccga aggtctcccg ggtcttcctc gcccccatcg gcctcgccat
     9001 gctcctcttc gccgtggcaa ccctcctcgg gtaccccgag ggggccacgg tgatcgtcct
     9061 cggggtggtc ggcgtctaca tcctcttccg ggtcttcggc gtcgactctg cctttggcgc
     9121 ggggttttcc tccctccagt cctcccttga gaaggggcgg ttctccttcg tcacctccat
     9181 ctcggccatc gtccttgccg tgatcgggat cgttctcggc tcggcctcgg tgttcgagaa
     9241 ttacacctcc gagttcacca tgggcatctt cctctatgtc atctcctttc tctatggatc
     9301 cctcggctgg ttcaccgcct cggtgctcat catcaccgcc gggaaggtca tcgacgtata
     9361 catgaacgac ccggccggcc tcgcgaggac catcgtcctc cccttctttg ctggctccat
     9421 cgggctcatc gcctacgggg cgagcgtcta caccctctcg gtgtcgaacc tcctcacctt
     9481 tccctatacc ccgagccagg gctcccagtt catcaccctc gcggtagtgg ggggcctcct
     9541 cgtggccatc atcggggtgt acgcccagtc cctggtcggc cggtggacca gggaaaagac
     9601 cgggacatcc gcagaataga aaccttttac gacccggccg agaagtaccc gaaactcttc
     9661 gggatcgacc ctgcgtcggg gaagaacagg gagtatccgg gagccctgac ctccaccgcg
     9721 gggaaggtca ccccgaagcg gtgaccgggg aaccagtagg catggtcccc gtagtcctcg
     9781 gcggcatggt ccaccaggaa gacggcatgg gtgaggccga cctcctcggg ccttgccgag
     9841 ggaaaattga gggatcgctc tatcttcttc tggagatccc ccgagccgaa gaggaggatg
     9901 atcaggccca tccccgggtc gacagagtag tccacgtcta cccgggcgtt cccgtcgagg
     9961 agctggaccc tcgcctcctc gaaggtgacg taccccaccc ggttcccctc tgcagcgagg
    10021 gcagggtatg cgatccccac gagaaggagc accaggagta ccggaagaga cgagcgcatg
    10081 ggcctttgag aatcgctccc gggattataa atcgtttacg gtcgtttttt cgccttcggg
    10141 gccggatcct ggacttccta tttaaagggg tgcggttccg tcccttcccc ctctccggta
    10201 atccggggaa ggggggggca tttcccctcc ggatcgggaa aagaaggcgg atggcgggtc
    10261 ttcctgagca gggatgaaaa ggcctgcctt cacttcaggc tgtagtagag ggcgatgaag
    10321 ccgatgatca ccgcaaccgc ataccccagg tacgcgagga agaggagggg gccggtgatg
    10381 cttgtccccg aggcgaagat gacgatggag gagccaacga cgagggccgc ggtgataagc
    10441 ccgacgagga tccgatcgct cgcccggtcg gagatgtgct ggacccggcg gaggtcctcc
    10501 tcggcgatct cgacccggag agacccggtg gagagccttt ttaagaccga gttcatgttc
    10561 agcggaagct cggcgatccc ctcgatggcg gttcgggcgg actcgcgggc tctcgagagg
    10621 accttccgcc ccgagtactc cttctcggcg agatccccca tgtacggctc gacgtacgag
    10681 ctgaagttga actgggggtc gaggctcttc ccgatgtccg ccaccatcat gatcaccttg
    10741 agcatgagca tgaggttcat gggcacctgc atctggtacc gtctcattac atcggaaagc
    10801 tcctggatca cctgggagat gttcacctct ttgaacctgt actcgctgta gtccatgagc
    10861 acgatgtaga ggtcgtccct caggttatcc atgtcctccg cccggacccg tacaccgagc
    10921 ctctcgaggg aacggaggag agtctccacg tcgttctgga ggatggaggc gaggaggttc
    10981 gtgaagatga gcctcttccc ggggtggatg atccccatga tcccgaaatc gaggaagtag
    11041 aggtccccct tcggggtgac gatcaggttc cccgggtggg gatcgccatg gaaaaaaccg
    11101 tcctcgaaga tctgcttcat gtacgcgtag aaaccgcggg tcccgatctc ccgggggctg
    11161 ataccgaacc cccggatccc gttggcgtcg tcgatccgga ccccctccac gtactccatc
    11221 acgaggagcc gggcccccga atacttccag tagatcttcg gtgtccggac gccctgcacg
    11281 tccctcatat tctgcccgag ccgctcggcg tttttcccgt cgcggacaaa atcgagctcc
    11341 ttccggatct gccgggagaa gtcctctacg atgcccgtgg ggttgtagac cctgaggtcg
    11401 gggaagaccg cctccaccct ctttgcgagg gcctggagga tgacgaggtc cttctcgatg
    11461 acctcggcga tgcccggccg ctggaccttg agggcgacct tcgtcccgtc cttgaggaca
    11521 ccccggtaga cgagggcgat ggaggcggag ccgacgggct cctcatcgat ctgctcgaag
    11581 acatcctcgg tgcagccgca atactcctcg aaaacgggct tcacccggga gaagggggcg
    11641 ggcgccaccc ggtcctggag ctggtggagc tcccggatga gctccggggg gaggatctcg
    11701 gtgcgggtgg aggcgatctg cccgaacttg atgaaggtgg gcccgagctc ctcgatggcg
    11761 agcctcatcc gctcgtatac cggccgctgc tcggggaaat gcttctcgaa aaacttccac
    11821 ttccttaccc cgggaaagag ctcgtcgaga gcgatcccga aaccatactt cacgagcaca
    11881 tccgcgatct gcccgtagcg ccggaaccgg gataccatga cacgatgttt ggatctgggg
    11941 gatgaaagaa gtttggggtt atttcacgcg aagaaaggga gtaactgtta tgggaaaatc
    12001 cgaatcagga ttgcctggat atcctcgttt tactctgggt gtgctctcgg gg
//
LOCUS       DATGDI010000084         4584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_182534, whole genome shotgun sequence.
ACCESSION   DATGDI010000084 DATGDI010000000
VERSION     DATGDI010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182534"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VKO45_03150"
     CDS             complement(<1..182)
                     /locus_tag="VKO45_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04901.1"
                     /translation="MVREERELQAQFSKRERTRKLLDNLEKLNSTGSLGPEQYAQMKK
                     GYEGAIAEAGTGIGSI"
     gene            complement(321..743)
                     /locus_tag="VKO45_03155"
     CDS             complement(321..743)
                     /locus_tag="VKO45_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04902.1"
                     /translation="MERNTLWKGAFVLAVTVLLLLLSGCSSTGGSTGLKSGSNSVVGT
                     WKADTSQGATTAITVVTFRADGTGQYKFDSTTPGYSQMDWHISYPFTWRVTDESYAGS
                     YVKAIGIVDDKNLPGPQFIYDTQRDRLTQSAQPFVRVS"
     gene            complement(750..1676)
                     /locus_tag="VKO45_03160"
     CDS             complement(750..1676)
                     /locus_tag="VKO45_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04903.1"
                     /translation="MAREERDLQRAYSEKEKYEKLLSNLDALKGKGAVSDDQYTSMKS
                     GYQRTQEQAVATVSQLKMKVTQEIQTEETNVERQNTELQTLDTRFKVGEIKADDLQKA
                     EQRIRGKIQRSQATIKDLNRLLSAQSSADVGGYVDVKPGSRSGGSASLPGLSIPGIGE
                     ALSAIRGTSPSDFTEIRTDTQEILTPSRELIGPAGGVLLFISLFLPWVSVFGFGANLF
                     AISGLYGAICLLAALAGIGSAFLVRENARGTAQFAGGAIALVVIILGFFIDPRTITQF
                     GTLMSFLGIGFYFFVIATLMMIVGGIMELGKR"
     gene            complement(1847..2719)
                     /locus_tag="VKO45_03165"
     CDS             complement(1847..2719)
                     /locus_tag="VKO45_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 12 protein"
                     /protein_id="HMA04904.1"
                     /translation="MFIGVDHGTTAIRFSSGEGEFRIPRKEAVDFAIRDLERLCPLGE
                     IEGIAVCYSMGDNFSEITPIALLRNRGVVSQAGAGEHVGGGTRVFDEVLGSGLPAVAI
                     PGLHRGSPTDPRFKAYSHQASPEKIGIAYEVHRSLGDDFVVSDVSSNTVSILVSGGKI
                     RGAIDACIFAPGTTQGALDVDAIRRIDEGEITANRAFMQAGVEFTVPEEERLRTLALF
                     SAMECAALRVLFPGAPVALAGTCAPVIAAEVEALLGVRPAVFDEWCASRGLARIARDV
                     FGGKREILGIGVRR"
     gene            complement(2913..3041)
                     /locus_tag="VKO45_03170"
     CDS             complement(2913..3041)
                     /locus_tag="VKO45_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04905.1"
                     /translation="MEITFLNVDDDDTECKDCGKKFKRQRKKDQCPECGSEKLSPL"
     gene            complement(3176..>4584)
                     /gene="ligD"
                     /locus_tag="VKO45_03175"
     CDS             complement(3176..>4584)
                     /gene="ligD"
                     /locus_tag="VKO45_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HMA04906.1"
                     /translation="AREAMSGPRYSPMLARTAEAPFSSPDWIFEVKWDGIRAIAYIRE
                     TLALLSRSGRDLSGQFPELREIRDLAPGTVLDGEIIVLANGRNDMQALLGRFQETDPE
                     KIRSDSLTLPATYVAFDILEADGKPLLRLPLEERQRILGERVKEGKYLVRSVSLRGDG
                     EPYYAEAVRRGLEGIMAKRLGSPYEPGVRSDSWLKVKALRTCDCVVFGYTRGEGARAG
                     TFGALVLGLFDDGKAVPVGRVGTGFDGRDLEGILASLAPFRSERPTLAGAERGEITWT
                     VPHMVIEVAYQAVTREGRLRMPRFLRIRTDKAPEECTADQLGPGRNLLPYRVKRDFAT
                     TPEPKGKGSPGPEGNAYVVHEHHARHLHYDLRLEREGVLKSWAVPKGVPEVTGERHLA
                     VETEDHPLEYRTFEGTIPEGEYGAGTVSIWDAGTYDTLAWTEGKIEVVFHGKRLSGRY
                     ALIRFRRPGKKDWLLIKT"
BASE COUNT          815 a         1466 c         1389 g          914 t
ORIGIN      
        1 ttgatactcc cgataccggt gccggcctcg gcgatggcac cctcgtagcc cttcttcatc
       61 tgggcatact gctcggggcc cagggagccg gtgctgttga gtttctccag gttatcgagg
      121 agcttccggg tcctttccct cttcgagaac tgggcctgga gctccctctc ctctctcacc
      181 atgattccac accggaaagg gtgttttaaa aatatcttat ttatctgttg ttgtttgcgt
      241 gtccccggcg gcgggaaatg aaccgtatcc atccggcctt gtccaaaacg cgagagttcc
      301 aggggagaaa agggggaaag ttatgatact ctcacaaagg gttgggctga ttgggttaac
      361 ctgtcccgct gggtatcgta aatgaattgg ggacccggga gatttttatc atcaacaatg
      421 ccgatggcct tcacgtatga acctgcataa ctctcatccg tcacccgcca ggtgaaggga
      481 tagctgatat gccagtccat ctgggaatag cccggggtcg tcgagtcgaa cttgtattgc
      541 cccgtcccgt cggcacgaaa cgtcacgacc gttatcgcgg tggtggcccc ctgcgacgtg
      601 tcagccttcc aggtgcccac aaccgaattc gatcccgact tgaggcccgt ggaaccgccg
      661 gtggatgagc agccggaaag gagaaggagg agcacggtga ctgcgaggac gaaggctcct
      721 ttccacagag tattcctttc catgccccgt caccgcttcc cgagctccat gattccgccg
      781 acgatcatca tgagggtggc gatgacgaag aagtaaaagc cgataccgag gaaggacatg
      841 agggtaccga actgggtgat ggttctcggg tcgatgaaga agccgaggat gatgacgaca
      901 agggcgatgg caccccctgc gaactgggcg gtcccccggg cattctccct cacgaggaag
      961 gcagacccga taccggcgag ggcggcgagg aggcagatgg ccccgtacag cccactgatg
     1021 gcgaagaggt tcgccccgaa cccgaagacc gatacccagg gcaggaagag gctgatgaag
     1081 aggagcactc cgccggccgg gccgatgagt tcccgggagg gtgtcaggat ctcctgcgtg
     1141 tccgtccgga tctcggtaaa atccgagggc gaggtcccgc gaattgccga gagggcctcg
     1201 ccgataccgg ggatggacag gcccgggaga gaggcggaac ctccggagcg tgagcctggt
     1261 tttacgtcga cgtacccgcc gacgtctgcc gaagactggg ctgagaggag gcggttcaga
     1321 tccttgatgg tggcctggga cctctggatc tttccacgga tacgttgctc tgccttctgc
     1381 aggtcgtctg ccttgatctc ccccaccttg aaccgggtgt caagggtctg gagctccgtg
     1441 ttctgccgct ctacattcgt ctcttccgtc tggatctcct gggtgacctt catcttcagc
     1501 tggctgacgg tcgcgaccgc ctgctcctgg gttctctggt acccgctctt catcgaggta
     1561 tactggtcat cggataccgc accctttccc ttcagggcat cgaggttgga gaggagcttc
     1621 tcgtacttct ctttctcgga atatgccctc tgtagatcgc gttcttcccg cgccatgagc
     1681 taaaagttcc gtagacacct atttatgtct acttaaatct ttcgtacacc cgctcgcagg
     1741 attggtttcc cgggagcacg acggtgcaat ggccttggtt acgagttttt agattgtcaa
     1801 caaggcaaag tcctgttccg ccgactttcc cgaagaagcc ggtcccctat ctccgcaccc
     1861 cgatccccag gatctccctc tttccgccga agacgtcgcg ggcgatccgg gcgaggcccc
     1921 gtgatgcaca ccactcgtcg aagaccgcgg gcctcacgcc gaggagggcc tccacctccg
     1981 ctgcgatgac cggggcacag gtcccggcga gagcaacggg ggccccgggg aataggaccc
     2041 ggagggccgc gcactccatc gccgaaaaga gggcaagggt gcggagcctc tcctcctccg
     2101 ggacggtgaa ttccaccccg gcctgcatga aggcccggtt cgcggtgatc tcgccctcgt
     2161 cgatcctccg gatggcatcc acgtccagcg ccccctgggt ggtgcccggg gcgaagatgc
     2221 aggcatcgat ggccccccgg atttttccac cggaaacgag gatgctcacg gtgttcgacg
     2281 acacatccga gaccacaaaa tcatccccca gggaccggtg aacctcgtac gcgatcccga
     2341 tcttctccgg gctcgcctgg tgggaatagg ccttgaaccg gggatccgtg ggggagcccc
     2401 ggtggaggcc agggatggca accgccggga ggccgctccc gagaacctcg tcgaagaccc
     2461 gggttccgcc ccccacgtgc tctccggccc ccgcctggct caccaccccg cggttcctga
     2521 ggagcgcgat gggagtgatc tccgagaagt tgtctcccat cgagtagcaa accgcgatcc
     2581 cttcgatctc cccgagtggg cagaggcgtt ccaggtcacg gatggcgaag tccaccgcct
     2641 ccttcctggg aatcctgaac tccccctcgc cggacgagaa gcggatggcc gtggttccat
     2701 ggtcgacgcc gatgaacatg atccctctcc agaggtgccc tcttcacgga ttcccgcgga
     2761 ctgtcagatc cttatgccat gtccggggag ttatagcctc cggtatcggc ggggcggtgt
     2821 ggatggcagg gaaaaaggta tttccggccc acgttccctg gcgatggggg aagaccattg
     2881 gattgggaaa aaaagggatt ttggtattgc ccctagagcg ggctcagctt ctccgagccg
     2941 cactcggggc attgatcctt cttcctctgg cgtttgaact tcttcccgca gtccttgcat
     3001 tcggtgtcat catcgtctac attgaggaat gttatctcca tgggtccgcc ggcagaacag
     3061 gccatatact ccacctcccg gcacgggatg atatcccatg ccgggatatg aagcttgccc
     3121 ctacttttca ccggccttca agatatcccc tcattgaggg gatttcgaaa aaccatcagg
     3181 ttttgatgag cagccagtcc ttcttcccgg gcctccggaa acggatgagg gcgtacctgc
     3241 ccgagagcct tttcccgtgg aagaccacct cgatctttcc ctcggtccag gcaagggtgt
     3301 cgtacgtccc ggcgtcccag atggagacgg tccccgcccc gtactcccct tcagggatgg
     3361 tcccctcgaa agtgcggtac tcgaggggat ggtcctcggt ctcgacggcg aggtgcctct
     3421 cccccgttac ctcggggacc cccttcggga ccgcccagga tttgagcacc ccctcccgct
     3481 ccagccgcag gtcataatgg aggtgccggg cgtgatgctc atgcacgacg taggcattcc
     3541 cctcaggccc tggtgacccc ttccccttcg gctccggggt cgtggcaaag tctctcttca
     3601 cccggtaggg gagcaggttt ctgcccggtc ccagctgatc ggcggtgcat tcctcgggag
     3661 ccttgtcggt ccggatccgc aggaaccggg gcatgcggag ccggccctcc cgggtcaccg
     3721 cctggtacgc aacctcgatc accatgtgag gaacggtcca ggtgatctct cctcgttcgg
     3781 caccggcgag ggtgggcctc tccgaccgga aaggggcgag ggaggcgagg atcccctcca
     3841 ggtccctccc gtcgaagccg gtgccgaccc tgcccacggg caccgccttt ccgtcgtcga
     3901 agagcccgag gacgagggcc ccgaaggtcc cggcccgggc cccttcaccc cttgtatacc
     3961 cgaaaaccac gcagtcgcag gtcctgaggg ccttcacctt cagccatgaa tcgctcctca
     4021 cccccggctc gtaggggctc ccgaggcgct tggccatgat gccctcgaga ccgcgtctta
     4081 ccgcctcggc ataatagggt tccccgtccc cgcgaaggga gacggagcgg accaggtatt
     4141 ttccctcctt caccctctcc ccgaggatcc tctgccgttc ctcgaggggg aggcgcagga
     4201 ggggctttcc gtccgcctcc aggatgtcga aggccacgta cgtcgccgga agggtgaggg
     4261 aatctgaccg gatcttctcc gggtctgtct cctggaaccg gcccaggagg gcctgcatgt
     4321 cgttccggcc gttggcaagg acgatgatct ccccgtcgag gaccgtcccg ggggccagat
     4381 cccggatctc ccggagctcc gggaactgcc cggagagatc cctcccgctc ctcgagagga
     4441 gggcgagggt ctccctgatg taggcgatgg cccggatccc gtcccacttc acctcgaaga
     4501 tccagtccgg ggaggagaac ggggcctccg ctgtccgggc gagcatcggg ctgtaccggg
     4561 gaccgctcat tgcttcccga gctt
//
LOCUS       DATGDI010000085         5234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_298994, whole genome shotgun sequence.
ACCESSION   DATGDI010000085 DATGDI010000000
VERSION     DATGDI010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298994"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..1159)
                     /locus_tag="VKO45_03180"
     CDS             complement(248..1159)
                     /locus_tag="VKO45_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMA04907.1"
                     /translation="MAREGWCLRVPRSKGEERRLELLREGLLDTSLLLKREGDELLLP
                     LKEPRDGCGRALFEEAPDRPDLPRHDLIGGIAVLLEGGEPEAELLLRSRPSIHTVLSP
                     EGPVEGEFRTRKFTVLTGDPTTRTSYIEYGLRFEIDLSLAYFSPRLSGERQRVLVLMN
                     TGERVLDMFAGVGPFAITLAEKAGVVYAADLNPGAVELMLGNVALNRVRNVLPFLADA
                     ARLPDILPRGFDRVVMNLPFGAMGFLDAALALCRPGGTIHLYAMVGEEGALSSTLRAK
                     GLEVLGERVVHSYSPAEWLAVYDLRVG"
     gene            1206..2423
                     /gene="hmgA"
                     /locus_tag="VKO45_03185"
     CDS             1206..2423
                     /gene="hmgA"
                     /locus_tag="VKO45_03185"
                     /EC_number="1.1.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase (NADPH)"
                     /protein_id="HMA04908.1"
                     /translation="MDQYLERLKNGTLKLYALEKEVPAPEAIRIRRAFIEQEAGVKLP
                     KLGSFTMDPSVAVQRNIENMVGAVQVPVGVAGPLKVRGEYADGEYYVPLATTEGALVA
                     SVNRGCGAITRAGGADVRILRNGMTRAPVFSTRDILHARAVKDFVDGNREALAAAAAE
                     TTSHGELTGAMAFVTGTTVHVRLEFDTKDAMGMNMVTIASERIGNLIEEKTGARLLAL
                     SGNLCTDKKPAAVNGILGRGKTVVAGVLLSGGLVSEVFKTDARTIAEVNYRKNLLGSA
                     RAGSLGFNAQAANIIAALFIACGQDAAHVVEGSGAFTTAEERDEGVYVSVTLPALQLG
                     TVGGGTSLDTQRECLSILGVAGGGNPPGANAKRFAEIAASAVLAGELSLLGALGAGHL
                     ARAHRALGRGKKI"
     gene            complement(2420..2881)
                     /gene="rimI"
                     /locus_tag="VKO45_03190"
     CDS             complement(2420..2881)
                     /gene="rimI"
                     /locus_tag="VKO45_03190"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HMA04909.1"
                     /translation="MSEQVHLRRARPTDIPGVMAIERECFVEPWDEEVFVQTLEWAPT
                     YFFVAVADGDIRGFVVGCMENTGSAVYGHVSNLGVTAAYRKKGIGRLLVARLERQYIL
                     EGAEGVLLEVRISNTEAQEFYHRLGYQEAFLLSGYYSNGEDALVMMKDTPF"
     gene            2957..4207
                     /locus_tag="VKO45_03195"
     CDS             2957..4207
                     /locus_tag="VKO45_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HMA04910.1"
                     /translation="MVQIHPFAALRPDPAFAPGVASVPYDVISAAEAGECIRKNPNSF
                     LRVSRADAELPGIPPHDEAVYIRARENLLSMERDGILIRDTEPSLYLYQVEDGGRLVT
                     GLVCTIGIGDYLDGTIRSHELTRRDKEEDRTRHIDAVNAQTGLVFLFYRDGGGIGPAI
                     GSLVSPGVPVATVEEDSGRIHRVFRVRDPAGIERATRLVLPLTRLYIADGHHRAAAAR
                     NVAMARRRDGRGGGEADRFMAVLFPHDQVAIHGYSRLVTDLAGMDPATLLNRLSKRFQ
                     VRKCFPEEAAPPSGPEGSGIHLFRMYLTGGWYEVSRAKASGSHGRNAALDVQVLQDEI
                     LGPLLGIREPREDPRLQFLGGARPVADLEARVDGGEFAVAFSLRPIGIGEVMAVADAG
                     GILPPKSTWFEPKLKSGLLVHTLD"
     gene            4257..5129
                     /gene="hisG"
                     /locus_tag="VKO45_03200"
     CDS             4257..5129
                     /gene="hisG"
                     /locus_tag="VKO45_03200"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HMA04911.1"
                     /translation="MITLALPKGSLEQQTLQLFREADLEVKRTERDYNPAIEDPRIGK
                     VKLLRPQEIPTYVAMGYFDLGISGQDWVRETGSDVVEAARLSYSKSGEGNVRIVIAVH
                     RDEPMEKASQIRPGSRVTTEYPELTRRYFGELGIPVQLFPSYGASEAKVPDLMDVVVD
                     LTETGTTLRRNGLKIIGEIMESSTVLIANRASFADPGKHREIDEIRTLLLGVIEGRHQ
                     VLITMNVPGPSLERVIRMLPAMKKPTVSRLHGIDYYSIQTVVPKAGVNQLIPGLKDAG
                     AEDILEIPISKIVR"
BASE COUNT          957 a         1723 c         1718 g          836 t
ORIGIN      
        1 aggaggtttc gtaagaaacc gaccttgagc gacgaagttg cgagtgctac gccccccgcc
       61 gcacgggcac ccccctccgt ttccgggaac tcccgatgta acggtgcagg ggatcaacgc
      121 ctcatcgccc ttggggacgc cacagcggcg ggggtgagcg gagcgaaccc ccaggagcgt
      181 ccgcgaatca acaacaaagt ccggatcggg agatagtttg aggatcttct gagattttaa
      241 ggggtattta tccgaccctc agatcgtaca ccgccagcca ctccgccggc gaataagagt
      301 gaaccacccg ctcccccagg acctcgagcc cctttgcccg aagagtggag gaaagagcgc
      361 cctcctctcc gaccatcgca tagagatgga tcgtcccgcc gggccggcac agggcgaggg
      421 cggcatccag gaaccccatt gccccgaagg ggaggttcat caccacccgg tcgaagccgc
      481 gcgggaggat atcggggagc cgcgcggcat cggcgaggaa gggaaggacg tttctcaccc
      541 ggttgagggc gacgttcccg agcataagct ccacggcacc cgggttgagg tcggcggcgt
      601 acaccacccc cgccttttct gcaagggtga tggcgaaagg accgaccccg gcgaacatat
      661 cgagcacccg ctctcccgtg ttcatgagca cgaggacgcg ctggcgttcg cccgagagcc
      721 ggggagagaa gtaggcgagg gagaggtcga tctcgaaccg gagaccatac tcgatatagc
      781 tggtcctcgt cgtgggatcc cccgtgagga ccgtaaactt cctcgtccgg aactccccct
      841 ccaccggtcc ctccggggag aggacggtgt ggatggacgg gcgggagcgg aggaggagtt
      901 ccgcctccgg ttccccgccc tccaggagaa ctgcgatacc gccgatcagg tcgtggcggg
      961 ggaggtccgg cctgtcgggg gcctcctcga agagagccct cccgcagccg tcgcggggtt
     1021 ccttcagggg gaggaggagc tcgtcgccct ccctcttcag gaggagggag gtatcgagga
     1081 gcccctcccg gaggagctcg aggcgccgct cctcaccctt cgatctcggg accctgaggc
     1141 accagccctc cctggccatg tcatccaccc tccagcttta atcctccggt tccccagagt
     1201 tgatcatgga tcagtacctc gagaggctaa aaaatggcac cctgaagctg tacgccctgg
     1261 agaaggaggt accggctccc gaggccatcc ggatccgccg ggccttcatc gagcaggagg
     1321 ccggggtgaa gctcccgaag ctcggatcct tcaccatgga cccttcggtg gccgtccagc
     1381 ggaacatcga gaacatggtg ggcgccgtcc aggtccccgt cggggtggcg ggacccctga
     1441 aggtccgggg cgagtacgcc gacggtgagt actacgtccc tctcgccacc accgagggtg
     1501 ccctggtcgc gtcggtgaac cggggatgcg gagccatcac ccgggccgga ggggccgatg
     1561 tgcgcatcct caggaacggg atgacccggg ccccggtctt ctccacccgg gacatcctcc
     1621 atgcccgggc ggtgaaggat ttcgtggacg ggaaccggga ggccctcgcg gccgccgccg
     1681 cggagaccac ctcccacggc gaactcaccg gcgccatggc ctttgtgacc gggaccacgg
     1741 tccatgtccg gctcgagttc gataccaagg atgccatggg catgaacatg gtcaccatcg
     1801 cgagcgagcg gatcgggaac ctgatcgagg aaaagaccgg ggcgaggctc ctcgccctct
     1861 cggggaacct ctgcaccgac aagaaaccgg ccgcggtgaa cggcatcctg ggccggggaa
     1921 agaccgtggt cgccggagtc ctcctctccg gcggcctcgt ctcggaggtg ttcaagaccg
     1981 atgcccggac cattgcggaa gtgaactacc ggaagaacct cctcggttcg gcccgggccg
     2041 ggtccctcgg cttcaatgcc caggcggcga acatcatcgc cgcccttttc atcgcctgcg
     2101 ggcaggacgc cgcccatgtc gtggaaggct cgggggcctt caccacggcg gaggagcggg
     2161 atgagggcgt gtacgtgtcg gtgaccctcc cggccctcca gctcgggacc gtcgggggcg
     2221 ggacctccct cgatacccag cgggagtgcc tctccatcct cggagtcgcc ggcgggggga
     2281 atcctcccgg ggcgaacgca aagcgctttg cagagatcgc cgcctccgcc gtcctcgccg
     2341 gcgagctctc cctcctcggg gccctcggtg caggccacct cgcccgggcc cacagggccc
     2401 tcggccgggg aaagaaaatt tagaaggggg tgtccttcat catgacgagg gcatcctcgc
     2461 cattggagta gtagcccgag aggaggaaag cctcctggta cccgagccgg tggtagaact
     2521 cctgggcctc ggtattcgag atcctcacct cgaggagcac cccctcggcg ccctcgagga
     2581 tgtactgcct ctcgagccgg gcgacgagga gccgcccgat ccccttcttg cggtaggcgg
     2641 cggtgacccc caggttcgag acatgtccgt acaccgcaga tcccgtgttc tccatgcagc
     2701 cgaccacgaa cccccggata tccccgtcag ccacggccac gaagaagtag gtgggggccc
     2761 actccagggt ctggacaaag acctcctcgt cccagggctc aacgaagcat tccctctcga
     2821 tggccatgac cccggggatg tccgtcggcc tcgctctccg gagatggacc tgctcgctca
     2881 tcgggcgcac tcggaagtag tgatttatga tacagggagt taaaatgtat acgctgcccc
     2941 cgatagggga ggttccatgg tccagatcca tcccttcgcc gcgctgcgcc ccgatcccgc
     3001 cttcgccccc ggggtcgcat cggtcccgta cgacgtgatc tcggccgccg aggcgggaga
     3061 gtgcatccgg aagaacccga actccttcct ccgggtgagc cgcgcggatg cggagcttcc
     3121 cggcatccct ccccatgacg aggccgtata catccgtgcc cgggagaacc ttctctccat
     3181 ggagagggat gggatcctca tcagggacac cgaaccatcc ctgtacctct accaggtgga
     3241 agatggcggc cgcctggtga ccggcctcgt gtgcactatc ggtatcgggg actacctcga
     3301 cggcaccatc aggagccacg aactcacccg ccgggacaag gaggaggaca ggacccgcca
     3361 catcgacgcg gtaaacgccc agacagggct tgtctttctc ttctaccggg atgggggggg
     3421 catcgggccg gcgatcggga gcctcgtttc ccccggggtc ccggttgcga cggtggagga
     3481 ggacagcggc aggatccacc gggtcttccg ggtccgggac ccggcgggga tcgagagggc
     3541 cacccgcctc gtcctccccc tcacccggct ctacatcgcc gatggacatc accgggccgc
     3601 ggcggcgagg aacgtggcga tggcgaggcg gagggacggg aggggcggcg gggaggcgga
     3661 ccgtttcatg gccgtccttt ttccccacga ccaggtggcc atccatggat acagcaggct
     3721 cgtgaccgac ctggcaggca tggatccggc tacccttctc aaccgtcttt ccaagagatt
     3781 ccaggtgaga aagtgcttcc cggaagaggc ggccccgccc tccggcccgg aaggttcggg
     3841 tatccacctc ttccgcatgt atctcaccgg gggatggtac gaggtgagcc gggcgaaggc
     3901 ctccgggtct cacgggagga atgcggccct cgatgtacag gtgcttcagg acgagatcct
     3961 cggacccctt ctcgggatcc gggaaccccg ggaggatccc cggctccagt tcctcggggg
     4021 tgcgaggcct gtcgcggatc tcgaggccag ggtggacggg ggggagttcg cggtggcctt
     4081 ctccctgcgg cccatcggga taggtgaggt catggccgtc gcggacgccg gcgggatcct
     4141 ccctcccaag tccacctggt tcgagccgaa gctgaagagc ggcctcctcg ttcataccct
     4201 ggactgaaac cggaaccccc catcctttat tccgtccggc actgatagat ccgggtatga
     4261 tcaccctcgc gctcccgaag ggcagccttg agcagcagac cctccagctc ttccgggagg
     4321 cagacctcga ggtgaagagg accgagcggg attacaaccc ggccatcgaa gacccccgga
     4381 tcggcaaggt gaaactcctc cggccccagg agattccgac ctatgtcgcg atgggctact
     4441 tcgacctcgg gatctcgggc caggactggg tccgggagac ggggtcagac gtcgtggagg
     4501 ccgcccggct ctcgtacagc aagagcgggg agggaaacgt gaggatcgtg atcgcggtcc
     4561 accgggacga gcccatggag aaggcctccc agatccggcc ggggagccgg gtcaccaccg
     4621 agtacccgga gctcacccgg cgctactttg gggagctcgg gatcccggta cagctctttc
     4681 cctcgtacgg ggcctcggag gcaaaggtcc cggacctcat ggacgtggtg gtggatctta
     4741 ccgagaccgg gaccaccctc cggcggaacg gcctgaagat catcggggag atcatggagt
     4801 cctcgacggt cctcatcgcg aacagggcga gcttcgcgga tcccggaaag caccgggaga
     4861 tcgacgagat ccggaccctc ctcctggggg tgatcgaggg acgccaccag gtcctcatca
     4921 cgatgaacgt accggggccc tcgcttgaaa gggtcatccg catgctcccc gccatgaaaa
     4981 agcccaccgt cagccggctt cacgggatcg actattacag catccagacg gtcgtcccca
     5041 aggccggggt caaccagctc atcccgggac tcaaggatgc cggcgccgag gatatcctcg
     5101 agatccccat ctcgaagatc gtgagataaa agaggccgac ggggatggac cgatgagggg
     5161 tatggaaagg agattccttt tccgggccgg tcaggggtgg accgtacccg tcccttcaaa
     5221 atcagatttt cata
//
LOCUS       DATGDI010000086         9666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_182711, whole genome shotgun sequence.
ACCESSION   DATGDI010000086 DATGDI010000000
VERSION     DATGDI010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9666
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182711"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1166)
                     /locus_tag="VKO45_03205"
     CDS             complement(132..1166)
                     /locus_tag="VKO45_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HMA04912.1"
                     /translation="MPWNSSRLEGCTLTGALSVTTEVAGAITVLHGPAGCAQHNVSLL
                     HATLLGEDTPRLLRILSSDLDEAGVIFGGEGALAASLREAAGRDPPVVFVLSTCVVQT
                     IGDDVDEVCRRGWGAPVIPVPSSGFLGGGFQDGMRNALIALGKRAGPSGSEGGTDGGI
                     TIVGEKNLEQDVDENFREVSRLLGLLGVKPGLRFVRGMEWGALGGLARGRLSILRDPE
                     LRPVGEYLASRYGTPYLGSFPVGLSGSLTFLREAARILGIDPREALEEERGRQKDALA
                     AFGDLKGERIRLSSSAGAGPDPFARELTESLGLVLGEGGVAMPLPSPLPVGTAGLVRL
                     LHRWRRVLHA"
     gene            complement(1141..1959)
                     /locus_tag="VKO45_03210"
     CDS             complement(1141..1959)
                     /locus_tag="VKO45_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMA04913.1"
                     /translation="MKQIALYGKGGIGKSTTAANLSAALAEMGLDILQIGCDPKRDST
                     RMLMHGSWIPTVLDQVREGREATITADQVVFRGFGGVRCVESGGPEPGVGCAGRGIIA
                     TFQLLEKLSSLTGDVVVYDVLGDVVCGGFAMPMREGYAQEVYLVTSGELMSLYAANNI
                     CKAIARLGGRARGRCRLAGVIGNAAGQAGEEALVRAFADRIHSRLITFIPRDPVVQRA
                     EINRKTVIEFAPESAQAGVYRSLAKSILENRDLAIPIPLSMDDLESLALEFLPA"
     gene            complement(2049..3008)
                     /locus_tag="VKO45_03215"
     CDS             complement(2049..3008)
                     /locus_tag="VKO45_03215"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HMA04914.1"
                     /translation="MNRGNFKEVIARSPRVFAIGVAGDSGSGKTTFTGAIREIFGKDL
                     VSSISLDDYHRYDREERKARGVTPLAPEANRIDRMERDLAAMKRGEPIEKPVYNHRTG
                     TFDPPVPFIPRKILILEGLHPLFTEGMRGLLDFSLFVDPDDEVKKFWKMKRDTGDRGY
                     RSDEVEAERAARAADYDRYIAPQRRFADAVISAGFSRYGRDLAGERNLYQVTLLQTRP
                     EERIRDIELSIDLSHLLSLSDRDFLMEFRLERLDGREMGALTFDGELPYALVRRLEAA
                     VERQTGVHPIRIFEHRSSVTAGEIVQLVLAWRIINRRIAMESC"
     gene            complement(3050..4234)
                     /locus_tag="VKO45_03220"
     CDS             complement(3050..4234)
                     /locus_tag="VKO45_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HMA04915.1"
                     /translation="MNSREIRPGVLAVGVVDWDRRIFDELIPTPEGTTYNSFLVKGSA
                     KTVLIDTVDPSKEGILHANLDELGVDRIDYLVSNHTEQDHSGSIPSILSRFPSAQVVA
                     TPRGKELLKLHLHIPDERIVTVEDRETLSLGDRTLEFIHAPWVHWPETMFTYLREDRI
                     LFTCDLFGSHYATDEPVIEDLSRVYRAAKRYYAEIMMPFRSVIKGHLEKIRPLPLDII
                     APSHGPAYRKPEQILAAYQDWVSDSVKNQVVIPWVSMHHSTEAMVRHLEKGLTDRGIE
                     VLPFHLTVTDIGELAMALVDAATVIIATPTVLTGPHPAAVSAAYLVKILKPKARSLGI
                     IGSYGWGGKTVKDLTGILQGFPAEVLEPVLAKGLPGPDDFRALDGLADRIAAKHRGYG
                     IL"
     gene            complement(4381..5046)
                     /locus_tag="VKO45_03225"
     CDS             complement(4381..5046)
                     /locus_tag="VKO45_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04916.1"
                     /translation="MAEEKKEFPWIQLIATVGTIMAALIGITPYLLKLNTPTTTPTIV
                     ILTLPPATTTPVPVTTAATTVETIPVTTQATAVTTIPTTGPPPTVTPGANETANTTVS
                     TTVPATTPVATPIPTTAPTIGTTTLPPGPPIHISVNTTSVIQGGFILATGSTDPSVSQ
                     VIITLRNSTTFQSAGVPVAPIQGAFQFEIGTAAYAPGDYTISAAVPDTQAMASATFVI
                     TAA"
     gene            complement(5371..5865)
                     /locus_tag="VKO45_03230"
     CDS             complement(5371..5865)
                     /locus_tag="VKO45_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HMA04917.1"
                     /translation="MIWKGSTIARAETFILAIGVCYGAPAIGSYFTGLSVSTWYAALA
                     KPPIAPAGWFIGAVWTVLYLLMGIALFLVLDADRSLPAVKKGISFFVLQLGLNVLWSV
                     LFFGLRSPLFALLGIILLWVSIAATLLLFLKVSRPAGFLLLPYILWVSFAAVLNAWIL
                     LLNP"
     gene            complement(5862..6140)
                     /locus_tag="VKO45_03235"
     CDS             complement(5862..6140)
                     /locus_tag="VKO45_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HMA04918.1"
                     /translation="MGCIEELKYETILSHASFKECRDYLKEHFTERFEVEPGFKIFDS
                     YIIGIPPILVAVEGDTVIFPYTKPCHGTFLLRIDCADEVARLRKEKKR"
     gene            complement(6119..6622)
                     /locus_tag="VKO45_03240"
     CDS             complement(6119..6622)
                     /locus_tag="VKO45_03240"
                     /inference="COORDINATES: protein motif:HMM:NF020570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1890 domain-containing protein"
                     /protein_id="HMA04919.1"
                     /translation="MADPVTGGKEGGETGRKTALLLLGCPEVPVQMGIALYIAGRLEK
                     GGFSVTVAGNPSVIHLIRVSDPDQHYIRKVMNLDRAMADLIEKRLAPDLIVSFAHNDA
                     GISYAATVRHVSASPLYLVIFGREAEALAGEAEFECEKIVERAVHNPVQLKKKIDGVF
                     GWAVSRS"
     gene            complement(6668..7102)
                     /gene="cutA"
                     /locus_tag="VKO45_03245"
     CDS             complement(6668..7102)
                     /gene="cutA"
                     /locus_tag="VKO45_03245"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HMA04920.1"
                     /translation="MSRPTEVRENRGCREGNIFLMGGSNLPVGSDGVKGDRVILVLST
                     VPESESARIAETLVSERLVACVNATPVRSTYRWKGEICRDREDLLIMKTRESLFARVR
                     DRVREIHPYEVPEILALPVWGGFEGYLRWVVVETTQPGKPEG"
     gene            7151..8299
                     /locus_tag="VKO45_03250"
     CDS             7151..8299
                     /locus_tag="VKO45_03250"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HMA04921.1"
                     /translation="MYSNRMGNLPPYLFAQIDELKEAQLQRGVDVIDLGIGDPDLPTP
                     PHIVEALCRAARDPANHHYPSYAGMKEFRDAVATWYSRRFHVNLDPETEVTALMGSKD
                     GIAHIPEAFVNPGEYVLVPDPGYPVYRTSTLFAEGRIHPLPLLEKNGYLPDLSAIPPG
                     VVKKAKLLFLNYPNNPTAATAPAEFFDGVVDFACEHDIVVVHDNAYSEVSFDGYEAPS
                     FLQAHEAFEAGIEMHSLSKTYNMTGWRIGMACGKMEIIKGLRQVKTNVDSGVFNAVQH
                     AAIAALTGPQDCIRESAEVYQERRDLLVKGLREMGFQVALPQATFYVWMAVENGFDFA
                     RRLLDEAGVVVTPGIGFGQFGEGYVRFAITRSTERIAEALERIRGLGL"
     gene            8296..9591
                     /gene="lysA"
                     /locus_tag="VKO45_03255"
     CDS             8296..9591
                     /gene="lysA"
                     /locus_tag="VKO45_03255"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HMA04922.1"
                     /translation="MNLPPALSVKGGHLHIGRHDTVTLAARFGTPLYVTDEDRIRARY
                     REVHSALSSRYPHVRVLYAAKANGNPAILAALAGEGAGADVFSAGELLLALGAGMDPD
                     RLLFNGSSKSAADLALAVEKGVRVSVDSLDELRELDRIAGEAGETAEISFRVNPALEV
                     PTHPKIATGLRTSKFGIPAGEILGAYRAALACGNVEPVGLHCHIGSQILEVEPFARAA
                     GVMTVLAAGLTDLGVRLDFIDLGGGLGVPYRHGTDPAPSFSDYASAVVPVFAAGVKKA
                     GIAPELWVEPGRYIVADSTILLAGVNSVKEAHRRFVNTDAGFNLLVRPVMYGAWHGAL
                     VANKADTPPAHRYTIAGPICETGDLLAEDRDLPAVEPGDIIAILDAGAYGFAMASQYN
                     GRPRCAEVLVRGPDTALMRRRETVEDLTAAVERPPWQGL"
BASE COUNT         1657 a         3112 c         3226 g         1671 t
ORIGIN      
        1 cccgtcatcc cgcagggcca gaggggggcc gcacactcag gcatggagca cctcctgctg
       61 gcaggcggag ggctgcatgg catcaggcat ggagcacctt cctttccaaa ggaatggagg
      121 gctgcatggc atcaggcatg cagcacccgc ctccaccggt ggagcaggcg gacgaggccc
      181 gcggtgccca cgggcagggg ggagggaagg ggcatggcca cccctccctc cccgaggaca
      241 aggccgaggg attcggtgag ctccctggcg aagggatcgg ggccggctcc ggcggaagag
      301 gagagccgga tccgttcacc cttcaggtcc ccgaaggcgg cgagcgcatc cttctgccgt
      361 ccacgctcct cctccagggc ctcccgggga tcgatcccga ggatacgggc ggcctcccgc
      421 agaaaggtga gggagcccga aagcccgacg gggaaggaac cgaggtacgg ggtcccgtac
      481 cgggaagcga ggtattcgcc gacgggccgg agctcgggat ccctcaggat ggagagcctc
      541 ccccgggcga gtcccccgag ggccccccat tccatccccc ggacgaaccg gaggccgggc
      601 ttcaccccaa gcagccccaa aaggcgcgag acctcccgga agttctcgtc cacgtcctgc
      661 tccaggttct tctccccgac gatggtgatc ccgccgtccg tgcccccctc actcccgcta
      721 gggcccgccc gcttccccag ggcgatgagg gcattcctca taccgtcctg gaacccccca
      781 ccgaggaacc cggaggacgg cacggggatg accggggccc cccagccgcg ccggcagacc
      841 tcatccacat cgtccccgat ggtctggacg acgcaggtag agaggacgaa gacgaccggg
      901 ggatcccttc ccgcggcttc acggagggag gccgcgaggg ccccctcccc gccgaagatc
      961 accccggcct cgtccaggtc ggaggagaga atccggagga ggcggggggt atcctcgccg
     1021 aggagggtgg cgtggaggag ggagacgttg tgctgggcgc atcccgcagg gccgtgcagg
     1081 acggtgatcg cgcccgccac ctcggtggtc accgagaggg cgcccgtgag cgtgcagccc
     1141 tcaagccggg aggaattcca gggcaaggga ttcgaggtca tccatggaga gggggatcgg
     1201 gatcgccaga tcccggtttt cgagaatgct cttcgcgagg ctccggtaca cgccggcctg
     1261 ggccgattcg ggggcgaact cgatgacggt cttccggttg atctcggccc gctggaccac
     1321 ggggtcgcgg gggatgaagg tgatgagccg ggaatggatc cggtcggcga aggcccgcac
     1381 gagggcctcc tcgccggcct ggccggcggc gttcccgatg accccggcga gccggcagcg
     1441 tccccgggcc cggcccccca gtcgggcgat ggccttgcag atgttgttcg ccgcgtagag
     1501 ggacatcagc tcgcccgagg ttaccaggta cacctcctgg gcgtacccct cccgcatcgg
     1561 catggcgaag cccccgcaga ccacgtcgcc gaggacatca tagacgacga catcccccgt
     1621 gagagatgag agcttctcca ggagctggaa ggtggcgatg atcccccgcc cggcgcaacc
     1681 cacccccggt tcaggcccgc ccgactcgac gcaccgcacc cccccgaacc cgcggaagac
     1741 cacctggtcg gcggtgatgg tggcctcgcg tccctcccgg acctggtcga ggaccgtcgg
     1801 gatccagctt ccgtgcatga gcatccgggt gctgtcccgc ttggggtcgc agccgatctg
     1861 gaggatgtcc agccccatct cggcgagggc ggccgacagg ttcgcggccg tggtggactt
     1921 cccgatcccg cccttcccgt agagggcgat ctgcttcatc ctctacccgg taggccgggc
     1981 ttgtatatag attacttgat ctagaacaag taaatttaat tatcaaatat agtataaatg
     2041 ataacttttt agcaggattc catggcgatc cgacggttta taatgcgcca ggcgaggacc
     2101 agctggacga tctctcccgc ggtgaccgac gagcgatgct cgaagatccg gatggggtgg
     2161 accccggtct gccgctcgac tgcggcctcc agcctccgga ccagggcgta ggggagctcc
     2221 ccgtcgaagg tcaatgcccc catctccctc ccgtccagcc tctcgagccg gaactccatg
     2281 aggaagtccc ggtcggaaag ggacaggagg tgggagagat cgatggagag ctcgatgtcc
     2341 cggatcctct cctccggcct cgtctggagg agggtgacct ggtacaggtt cctctccccc
     2401 gcgaggtccc tcccgtaccg cgagaacccc gccgagatca ccgcatcggc aaacctccgc
     2461 tggggtgcga tgtatcgatc gtaatcggcc gcccgggcgg cacgttccgc ctcgacctcg
     2521 tccgacctgt acccccggtc ccccgtgtcg cgtttcatct tccagaattt cttcacctca
     2581 tcatcggggt cgacgaagag gctgaagtcc aggaggcctc gcattccctc ggtgaagagg
     2641 ggatggagcc cctccaggat gagaatcttc ctcgggatga aggggacggg gggatcgaag
     2701 gtcccggtgc ggtggttgta caccggtttt tcgatgggct ctcccctctt catcgcggcg
     2761 aggtcccgct ccatccggtc gatccggttc gcctccgggg cgagaggggt gaccccccgg
     2821 gccttccgct cctcccggtc gtagcggtgg tagtcgtcga gggagatgga ggagacgaga
     2881 tctttcccga agatctcccg gatcgcaccc gtgaaggtgg tcttccccga gccgctgtcc
     2941 cccgccaccc cgatggcgaa gacgcgggga gaccgggcga tgacctcctt gaagttcccg
     3001 cggttcatgg atataggagt gatcggaggc ggggatgatg aagatgcctt cagaggattc
     3061 cgtagccccg gtgcttcgcg gcgatccggt ccgcgaggcc gtcgagggcc cggaaatcat
     3121 ccgggccggg gagtcccttt gcgaggaccg gctcgagaac ctcggccggg aacccctgga
     3181 ggatgcccgt gaggtccttc acggttttcc ctccccagcc gtaggatccg atgatcccga
     3241 ggctcctcgc cttgggcttc aggatcttca cgaggtaagc cgcactgacc gccgccgggt
     3301 ggggcccggt gaggacggtg ggggtggcga tgatcaccgt cgccgcatcc acgagggcca
     3361 tggcgagctc gccgatgtcc gtgacggtga ggtggaaggg aagcacctcg atcccccggt
     3421 ccgtcaggcc cttctccagg tggcgtacca tggcttcggt ggaatggtgc atcgagaccc
     3481 aggggatgac gacctggttc ttcaccgagt ccgagaccca gtcctggtac gcggccagga
     3541 tctgctccgg tttccggtac gccgggccgt ggctcggggc gatgatgtcg aggggaaggg
     3601 gccggatctt ctccaggtgt cccttgataa cgctcctgaa gggcatcatg atctcggcgt
     3661 agtaccgctt cgcggcccgg tacacccggc tcagatcctc gatgacgggc tcgtctgtgg
     3721 cataatggga gccgaagagg tcgcaggtaa agaggatgcg gtcctcccgg aggtacgtga
     3781 acatcgtctc gggccagtgg acccagggag cgtggatgaa ctccagggtc cggtccccga
     3841 gggagagggt ctcccggtcc tccaccgtca cgatcctttc gtcggggata tgcaggtgga
     3901 gcttcaggag ctcctttcct ctcggggtag cgacgacctg tgcagatggg aaccgggaga
     3961 ggatcgaggg gatggacccc gagtggtcct gctcggtatg gttcgagacg aggtaatcga
     4021 tccggtccac cccgagctcg tcaagattgg catggaggat tccctccttc gatggatcca
     4081 cggtgtcgat gagcaccgtt tttgcgctcc ccttcaccag aaagctgttg tatgtcgttc
     4141 cttcgggggt ggggatcaac tcgtcgaaga tcctccggtc ccagtcgacg accccgactg
     4201 ccaaaactcc gggcctgatc tcacgtgaat tcatacatcc tctgctatcc tttgaaaggt
     4261 cgattctcct ttctccttgc ttcatttatg tatatcggat cccccgggtc ctgggaagat
     4321 tcggcatctc aggttcaccg ggatagatcc gcaggaaaga aggatgtact agaggccgct
     4381 ttacgctgct gttatcacaa aggtcgccga ggccattgcc tgggtgtccg ggacggcggc
     4441 ggagatggta tagtcacccg gggcataggc ggcggtgccg atctcgaact ggaacgcgcc
     4501 ctggatcggg gccacgggga caccggctga ctggaaggtg gtgctgttcc tcagggtgat
     4561 gataacctgg cttaccgagg gatccgtgga gccggtggcc aggatgaagc cgccctggat
     4621 aacgctcgtt gtgttcaccg agatatggat gggtggaccg ggaggcaggg tcgttgttcc
     4681 aatggtcggg gccgtcgtcg ggatgggcgt ggcgaccggg gttgtcgccg ggacggtcgt
     4741 tgagacggtc gtgttcgcgg tctcgttcgc gccgggtgtc acggtcgggg gaggcccggt
     4801 ggtcgggatg gtggtcaccg ccgtggcctg ggtggtgacc ggaatcgtct caacggtcgt
     4861 cgcggcggtg gtgacgggga ctggtgtcgt ggtggccgga ggaagggtca ggatgacgat
     4921 ggtcggggtg gtggtcggag tgttgagctt caggaggtaa ggggtgatgc cgatgagggc
     4981 agccatgatg gtacccaccg tggcgatgag ctggatccag gggaattcct tcttttcttc
     5041 tgccatatcc ggttcctgag gagctaaggt ctccctgtcg cttcttatgt gttgtgcatt
     5101 gatccaccgg tatccctctc gattcgcccc gtttcccgtc gcgcgggatg ggacgcggcg
     5161 cacagagagt ccggggagtc ggggcggaac ggtggctcac agccacccgc gggcctgcat
     5221 ggccccgacg accctcttca ccgcgatggt gtaggcagag gtgctgttcg acgatggaaa
     5281 ggaggttcgg ctcgtccatg gtgtcaccga atgtacttcg gaatgggagg atgggctatt
     5341 agaaccttat tgatgcagcg gtcccaggac ctatgggttg aggaggagga tccaggcgtt
     5401 caggactgcg gcgaaactga cccacaggat atagggaagg agcaggaacc ccgcggggcg
     5461 ggagactttc aggaacagga ggagcgtcgc cgcgatggac acccacagga ggatgatccc
     5521 gaggagggcg aaaagagggg accggaggcc gaagaagagg acagaccaga ggacattgag
     5581 gccgagctgg agcacgaaga aggagatgcc ctttttcacg gccggcaggc tccggtccgc
     5641 atcgaggacg aggaacaggg cgatccccat gagaaggtaa agcaccgtcc agaccgcacc
     5701 gatgaaccag ccggccgggg cgatgggggg ctttgccagg gccgcatacc aggtgctcac
     5761 cgagaggccg gtgaagtacg agccgatggc tggggccccg tagcacaccc cgatggcgag
     5821 gatgaaggtc tctgcccggg cgatggtact tcccttccag atcaccgctt cttctccttc
     5881 cggaggcgcg ccacttcatc ggcgcagtcg atccggagga ggaaagtccc gtggcagggc
     5941 ttcgtgtacg ggaagatcac cgtgtccccc tcgaccgcga cgaggatggg agggatgccg
     6001 atgatgtagg agtcaaagat cttgaaaccc ggttccacct cgaaccgctc cgtgaaatgc
     6061 tccttcaggt agtcccggca ctccttgaag ctcgcgtggg aaaggatcgt ctcgtacttc
     6121 agctcctcga tacagcccac ccgaacaccc cgtcgatctt cttcttcagc tggaccgggt
     6181 tgtgcaccgc ccgctccacg atcttctcgc actcgaactc ggcctccccc gcgagggcct
     6241 cggcctccct gccgaagatg acgaggtaga ggggggaggc ggagacatgc cggaccgtcg
     6301 ccgcatagga gatcccggca tcgttgtggg cgaaggagac gatgaggtcc ggggcgagcc
     6361 tcttctcgat gagatcggcc atggccctgt ccaggttcat gacctttctg atgtagtgct
     6421 ggtcaggatc ggagacccgg atcaggtgga tgaccgaggg gttcccggcc acggtgacgg
     6481 aaaatccgcc cttctcgagc cggcccgcga tgtagagggc gatgcccatc tggaccggga
     6541 cctccgggca ccccaggaga agaagcgcgg ttttccggcc cgtctcgcca ccttcctttc
     6601 cccccgtgac tggatccgcc atcacgtcac cccgcacccg gagggtgtgt ccccctcctt
     6661 catgagctta accttccggt tttcccggct gtgtggtctc caccaccacc caccggagat
     6721 atccctcgaa accaccccag acgggcaggg ccagaatctc ggggacctcg tagggatgga
     6781 tctcccgcac ccggtcccgg acacgggcga agaggctctc cctcgtcttc atgatgagca
     6841 gatcctcccg gtccctgcag atctccccct tccaccggta ggtagagcgg acgggggtcg
     6901 cgtttacgca tgcgacgagc ctctcggaaa cgagggtttc ggcgatgcgt gccgattcgg
     6961 actccggaac cgtcgaaagg acgagaatga cccggtcacc cttcacgcca tcacttccaa
     7021 cgggtaggtt actcccaccc atgagaaaga tgttcccttc cctgcatccc cggttctccc
     7081 tcacctccgt aggccggctc acctgcgaaa ttattatgaa ccggccccca aaggtagagg
     7141 aggatgtgag atgtactcga accggatggg caaccttccc ccctacctct tcgcccagat
     7201 cgacgagctg aaggaggcac agctgcaaag gggggtggat gtcatcgacc tcggcatcgg
     7261 cgaccccgat ctcccgacgc ccccccatat cgtggaagcc ctgtgcaggg cggcgaggga
     7321 cccggccaac caccactacc cctcgtacgc gggaatgaag gagttccggg atgccgtcgc
     7381 cacctggtac agccggcggt tccatgtcaa cctcgacccc gagaccgagg tcaccgccct
     7441 catgggatcg aaggacggga tcgcccatat accggaggcc ttcgtcaacc cgggcgaata
     7501 cgtcctcgtc ccagaccccg ggtacccggt ctaccggacc tccaccctct ttgccgaggg
     7561 gcgcatccac ccgctcccgc tcctcgaaaa gaacgggtac ctccccgacc tctcggccat
     7621 tccccccggg gtcgtgaaga aggcaaagct cctcttcctg aactacccga acaaccccac
     7681 ggcggccacg gcgcccgcgg agttcttcga cggggtggtg gacttcgcct gcgagcatga
     7741 catcgtagtc gttcacgaca acgcgtactc ggaggtttcc ttcgacggat acgaagcgcc
     7801 gtccttcctc caggcccacg aggccttcga ggcggggatc gagatgcact ccctctcgaa
     7861 gacctacaac atgaccggct ggcgcatcgg gatggcctgc gggaagatgg agatcatcaa
     7921 ggggctccgg caggtgaaga cgaacgtgga ctcgggggtc ttcaacgcgg tccagcacgc
     7981 ggccatcgcg gccctcaccg gcccccagga ctgcatccgc gaatcagcgg aggtctacca
     8041 ggagaggagg gaccttctcg tgaaggggct ccgggagatg ggcttccagg tcgcccttcc
     8101 ccaggccacc ttctacgtct ggatggcggt ggagaacggc ttcgacttcg cccgccggct
     8161 cctcgacgag gcgggggtgg tcgtgacccc gggcatcggc ttcgggcagt tcggggaggg
     8221 ctatgtccgg ttcgccatca cccgttcgac cgagaggatc gccgaggccc tggagcggat
     8281 acgggggctc ggcctgtgaa cctccctccc gcgctctcgg tgaagggagg ccacctccac
     8341 atcggccggc acgacaccgt caccctcgcc gcccggttcg ggaccccgct ctacgtgacc
     8401 gacgaggacc ggatccgcgc ccggtaccgg gaggtccact cggccctctc ctcccgctac
     8461 ccgcacgtga gggtcctcta cgcggcaaaa gcgaacggga accccgcgat cctcgcggcc
     8521 ctcgccggcg agggggcagg ggccgatgtc ttctccgcgg gcgagcttct cctcgccctc
     8581 ggggccggga tggacccgga ccggctcctc ttcaacggct cctcgaagag cgccgccgat
     8641 ctcgccctcg ccgtggagaa gggggtaagg gtctcggtgg actccctgga cgagctccgc
     8701 gagctcgacc ggatcgcggg ggaggccggg gagaccgcgg agatctcctt ccgggtgaac
     8761 ccggccctcg aggtgcccac ccacccgaag atcgccaccg gcctcaggac ctcgaagttc
     8821 ggcatccctg ccggtgagat cctcggtgcg taccgggcgg ccctggcgtg cgggaacgtc
     8881 gaaccggtgg ggctccactg ccacatcggt tcccagatcc tcgaggtgga gccctttgcc
     8941 cgggcggccg gggtgatgac agttctcgcc gcggggctca ccgacctcgg ggtccggctc
     9001 gacttcatcg acctgggggg cggtctcggc gtcccttacc ggcacggcac cgaccccgcg
     9061 ccctccttct ccgactatgc ctcggcggtg gtgccggtct ttgcggcggg tgtgaagaag
     9121 gcggggatcg cccccgagct ctgggtggag cccggccggt acatcgtcgc cgactccacc
     9181 attctccttg cgggggtgaa ttcggtcaag gaggcccacc ggcggttcgt gaacaccgat
     9241 gccggtttca acctccttgt ccggccggtg atgtacggcg cctggcacgg ggcgttggtg
     9301 gcgaacaagg cggacacccc cccggcccac cggtacacca tcgcgggccc catctgcgag
     9361 accggggacc tccttgccga ggaccgggat ctcccggccg tcgaaccggg ggacatcatc
     9421 gcgatcctcg acgccggggc gtacgggttc gccatggcct cgcagtacaa cggccggccc
     9481 cggtgtgccg aggttttggt ccggggcccg gatacggccc tcatgcggag gcgggagacg
     9541 gtggaggacc tcaccgccgc tgtcgagagg cccccctggc agggactctg acagctgggt
     9601 accgatgtcc ttccactacg ccctcgtgga tgacctcctc tcccgggagg agttcgaccg
     9661 gcgggt
//
LOCUS       DATGDI010000087         4558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_328120, whole genome shotgun sequence.
ACCESSION   DATGDI010000087 DATGDI010000000
VERSION     DATGDI010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328120"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VKO45_03260"
     CDS             <1..770
                     /locus_tag="VKO45_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HMA04923.1"
                     /translation="ILSRVSFMRVVFLGTNGWYDTETGNTTCTLVEAGPWRILLDAGN
                     GIRRLEGPLGDPDHPCHLFLSHFHIDHVAGLHVLGRLSFPGGLSIWGQPGTRETLATL
                     LAPPFTVPCGDLPFPVRICDLAEGAHRLPFPVTCRYLPHASPCFGYRFELEGRIIAYC
                     TDTGPSDTVIELARDADLLIAESALRPGTTSDDWPHLNPEMAVALARSAGARRLSLTH
                     FDAWAYRTLEERTRAGEEVRESFPDLTIATDGLVLDL"
     gene            879..1295
                     /locus_tag="VKO45_03265"
     CDS             879..1295
                     /locus_tag="VKO45_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HMA04924.1"
                     /translation="MDAKKGKTARGKAAPKRWRCKLCNYIYSPLIGEPHNGIPAGTAF
                     EDLPDSYVCPVCGQAGKGKVGKWGFEEWIPTRYHCKACGYVYDKARGEPHRGIKPGTA
                     FEDLPDTYVCPVCALDPDIVKDLGKVGKEGFEPILF"
     gene            complement(1444..1992)
                     /locus_tag="VKO45_03270"
     CDS             complement(1444..1992)
                     /locus_tag="VKO45_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04925.1"
                     /translation="MIRGRITEREGNFGKVLRTFAYESDGITEHEGDRLGRVKRALMY
                     EVDGKTLTEKLGGKDGKVKRTFTFEADHVVEWETSSYGKRKSTYLFDPGEHRILEREG
                     GKYGTLLRGFLFEPEGVIEGEGGGHGPVRRMFVFDREKPAIFERTGGHFGEIKRTFVF
                     EQMDPSVLRNFDAFLQFVFLVG"
     gene            complement(2049..3947)
                     /locus_tag="VKO45_03275"
     CDS             complement(2049..3947)
                     /locus_tag="VKO45_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HMA04926.1"
                     /translation="MKRKRPSPAPLPMTLEEAGERGMDDLDIILVTGDAYVDHPAFGT
                     AVIGRVLEAAGYRVGIIAQPDWRSRADFQRLGRPRLFFGVSSGNVDSMVNNYTAAGRR
                     RRTDAYSPGGIPRRPDRAAIVYADRLHALFPGVPIVLGGLEASLRRFAHYDYWSDRVR
                     KSILADAPADLLVYGMGERQVVEIARRLSEGEGSRPLVDIRGTSYRIAPKDFRETEAG
                     DIVILPSFAEVSEDPAAYSRGAPSPLDAPGSLRDPGAYARAFALHSLEQDPVRGRRVA
                     QDHGKTVIVQNPPAIPLSTEELDRVYELPYTRRGHLSYREEIPALEPVRFSLVSHRGC
                     FGGCSFCAIGHHQGRIIQSRSPESLVRETRRIVGMKGFAGVIQDLGGPTANMYGFTCA
                     RWTAAGTCEDRFCSLDCGNLVSGEKAHLALLRRIRAVPGVKKVFIASGIRHDLIDLSD
                     LEYLTELCAHHVGGHLKVAPEHISPRVLSLMRKPGRRAFDEFRRRFAEVNRRLGKEQY
                     LLPYFMSGHPGCTVADMVDLAEYIRDESLYTEQAQDFTPTPMTAATCMYYTGIDPFTG
                     EEVHVPRGREKEIQRAMLQYRDPAKAGFVREGLRIAGREDLAGSAWNCLVPAETGTRK
                     REKRRYLR"
     gene            complement(3989..4501)
                     /locus_tag="VKO45_03280"
     CDS             complement(3989..4501)
                     /locus_tag="VKO45_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HMA04927.1"
                     /translation="MNGVPEVTEKEIAALHERLDREAEEGGYHLNPDRAFVRGLVRGL
                     IINTRRYGYQSCPCRLATGEKKADLDIICPCDYRDPDLADFGACYCALYVSDEVLAGR
                     RKVASIPERRPPPDVRVRVPPSAGAAPSFTLEYPVWRCRVCGYLCARDEPPDVCPICK
                     AKKDRFERFL"
BASE COUNT          752 a         1549 c         1477 g          780 t
ORIGIN      
        1 gaatcctctc ccgagtatcc tttatgcggg tggtgttcct cgggacgaac ggatggtacg
       61 acaccgagac gggaaacacg acctgtaccc tcgtcgaggc agggccgtgg aggattctcc
      121 tcgacgcagg caatggtatc cggcggctgg agggtcccct cggggacccg gatcacccct
      181 gccacctctt cctctcccac ttccacatcg accacgtcgc cgggctccac gtcctcggcc
      241 ggctctcctt cccgggcggg ctctccatct ggggccagcc ggggacccgg gagaccctcg
      301 cgaccctcct cgccccgccc ttcaccgttc cctgcggaga cctccccttc cccgtccgga
      361 tctgcgacct tgccgagggg gcccatcgcc tgcccttccc ggtcacctgc cggtacctcc
      421 cccacgcctc cccctgcttc gggtaccggt tcgagctgga gggccggatc atcgcgtact
      481 gcacggacac cggtccgagc gataccgtga tcgagctcgc aagggatgcc gacctcctca
      541 tcgccgagtc ggcgctccgg cccggcacga cgagcgacga ctggccccac ctgaaccccg
      601 agatggccgt cgccctggcc cggtccgcgg gagcccgccg gctctccctc acccacttcg
      661 atgcctgggc ctaccggacc ctggaggaga ggacgagggc gggggaggag gtcagggaga
      721 gcttcccgga cctcaccatc gcgaccgacg ggctggtgct cgacctgtaa gggggggcct
      781 ctccccatcc cctgcataac ccataaatag gaacatcccc atctctcctg catggccgcc
      841 cgggcccgtt cccggcggac ccatcggagg ctctgaacat ggatgcaaag aaaggcaaaa
      901 cggcacgggg aaaggccgcc ccgaaacggt ggcgctgcaa actctgcaac tacatctact
      961 cccccctgat cggtgagccc cacaacggga tccccgcggg gaccgccttt gaggatctcc
     1021 ccgacagtta tgtctgcccg gtctgcggcc aggcagggaa ggggaaggtc gggaaatggg
     1081 gcttcgagga atggatcccc acccggtacc attgcaaggc ctgcgggtac gtctacgaca
     1141 aggcccgggg agagccccac cgggggatca agccgggaac ggccttcgag gatctcccgg
     1201 acacctatgt ctgccccgtc tgcgccctcg acccggatat cgtgaaggac ctcgggaagg
     1261 tggggaagga gggattcgag ccgattctgt tctagggcct ttgttttttc gcccaggagc
     1321 atggaataaa cccggcggtc ccggccaccc atttccgtta tttccctccc ggctcacggg
     1381 cagggacaag gaacggacgg tatgccaccc ttcagccggg gcggtccgcc gggaggtacg
     1441 ggttcacccg accaggaaga cgaactggag gaaggcgtcg aagttccgga gcaccgacgg
     1501 gtccatctgc tcgaagacga aggtccgctt gatctcgccg aaatggccac ccgtccgctc
     1561 gaagatggcc ggcttctccc ggtcgaagac gaacatcctc ctgaccggcc cgtggccacc
     1621 cccctcaccc tcgatgaccc cctcgggctc gaagaggaaa ccccggagga gggtcccgta
     1681 ctttcccccc tcgcgctcga ggatccggtg ctcccccggg tcgaagaggt aggtggactt
     1741 tctcttcccg taggagctcg tctcccactc gacgacatgg tcggcctcga aggtgaaggt
     1801 ccgcttcacc ttgccatcct ttcccccgag cttttcggtg agagtcttcc cgtccacctc
     1861 gtacatgagg gcacgtttca ccctcccgag ccggtccccc tcgtgctccg tgatcccgtc
     1921 gctctcatag gcgaaggtgc ggaggacctt cccgaagttc ccctcccgtt ccgtgatcct
     1981 tccccgtatc atggcagacc ttctcccggc cggccggcgg ccggttccgc tgaaaagagc
     2041 ttgaggagtt accgtaaata tcttctcttc tccctcttcc gggtgcccgt ttccgcgggg
     2101 acgaggcagt tccaggcgct ccccgcaaga tcctccctcc ccgcgatccg gagcccctcc
     2161 cggacgaacc ccgccttcgc gggatcccgg tactggagca tggcccgctg gatctccttc
     2221 tccctccccc ggggcacgtg gacctcctca ccggtaaacg ggtcgatccc cgtgtaatac
     2281 atgcaggtcg cggcggtcat cggcgtggga gtgaagtcct gggcctgctc ggtgtagagg
     2341 gactcgtccc ggatgtactc ggcgagatcg accatgtccg caacggtgca gccgggatgg
     2401 ccggacatga aataggggag gaggtattgc tccttcccga ggcgccggtt cacctccgcg
     2461 aacctccgcc ggaactcatc gaaggctctc cggccgggct tgcgcataag ggagaggacc
     2521 cggggggaga tgtgctcggg ggccaccttc aggtgcccgc cgacatggtg tgcgcagagc
     2581 tcggtgaggt actcgaggtc cgagaggtcg atgaggtcgt gccggattcc actcgcgatg
     2641 aacaccttct tcactcccgg gaccgcccgg attctccgga ggagggcaag gtgggccttt
     2701 tcgccggaga ccaggttccc gcaatcgagg gagcagaaac ggtcctcaca cgtcccggcg
     2761 gctgtccacc tcgcgcaggt gaacccgtac atgttggccg tgggcccccc gagatcctgg
     2821 atcacccctg cgaacccctt cattccgaca atacgtctcg tttcccggac gagggattcc
     2881 ggtgaccgac tctggatgat ccttccctgg tgatgcccga tggcgcagaa ggagcatccc
     2941 ccgaagcagc cccggtggct gaccagggag aacctcaccg gctcaagagc cgggatctcc
     3001 tcgcggtacg agaggtggcc ccggcgggtg tagggaagct cgtagacccg gtccagctcc
     3061 tccgtcgaca gggggatggc cggggggttc tggactatca cggtcttccc atggtcctgg
     3121 gcgaccctcc tgccccggac cgggtcctgt tcgagggaat ggagcgcgaa ggcccgggca
     3181 taggctcccg ggtcgcgaag ggagccggga gcgtccaggg gggatggggc ccctctggag
     3241 taggccgcgg gatcctccga tacttcggca aacgagggaa ggatgacgat gtccccggcc
     3301 tctgtctccc tgaaatcctt tggcgcgatc ctgtacgagg tcccccggat atcgacgagg
     3361 ggccgggatc cttcaccttc agagagtcgc cgggcgatct ccacgacctg gcgttccccc
     3421 atgccgtaca cgaggaggtc agcgggggca tcggcgagga tggatttcct cacccggtcg
     3481 gaccagtagt cgtagtgggc gaaacgtcgc aggctcgcct ccaggccccc gaggacgatg
     3541 ggtacccccg ggaaaagggc gtggagccgg tccgcgtaga cgatggccgc ccggtcgggg
     3601 cggcggggga ttcctccggg ggagtaggca tcggtccgcc tcctcctccc cgccgcggtg
     3661 taattgttca ccatggagtc gacattcccg gagctcaccc cgaagaagag gcggggacgc
     3721 ccgagcctct ggaaatcggc ccgggatctc cagtccggct gggcgatgat ccccacccgg
     3781 tatcccgcgg cctcgaggac ccggccgatc accgcggtcc cgaatgccgg gtggtccacg
     3841 taggcgtcgc cggttacgag gatgatgtca aggtcgtcca tcccccgctc cccggcctcc
     3901 tcgagggtca tggggagggg ggcaggggag ggccgctttc ttttcatggg cgcgggatcc
     3961 ggaaaaggga gggaggggag gatgttcgtc agaggaaccg ctcgaaccgg tccttcttcg
     4021 ccttgcagat cgggcagacg tcaggtggct cgtcccgggc acagaggtac ccgcagaccc
     4081 ggcatcgcca gaccgggtac tcgagggtga aggagggggc ggcccctgcg gagggcggga
     4141 cccgaacccg gacatcgggc gggggccggc gctccggtat ggaggctacc ttccttctcc
     4201 ccgcgagaac ctcgtccgag acgtacaggg cacagtagca ggccccgaaa tcggcgaggt
     4261 cggggtcacg gtagtcgcag ggacagatga tgtccaggtc agccttcttc tcccctgtgg
     4321 cgagccggca ggggcaggac tggtagccgt aacgcctcgt gttgatgatg agcccccgga
     4381 cgaggccccg gacgaaggcc cggtcagggt tgaggtgata gccgccctcc tcggcctccc
     4441 ggtcgagccg ctcgtggagg gcagcgatct ccttctccgt gacctcgggg acgccgttca
     4501 tccaagggcc tccctgtagc gggtctcatc gaacccgatg atacactgct ggttgttc
//
LOCUS       DATGDI010000088        10631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_124569, whole genome shotgun sequence.
ACCESSION   DATGDI010000088 DATGDI010000000
VERSION     DATGDI010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 10631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10631
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124569"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VKO45_03285"
     CDS             complement(<1..765)
                     /locus_tag="VKO45_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HMA04928.1"
                     /translation="MKPGAQAEWDPLRHVMVHEPGIEVFFALLAPEASLYERFFNHRE
                     ARWEHRQLCDLLHNNFGVRIHHLKATLLEEAGEPGPVRDLLVSLAARRLGGGRGPPAV
                     PLEERDPEHLFRIIVLNPDTGHGRAGGVRLRSPLHNLYFMRDQQAATDRGICLGRMAT
                     PERTEEVGVSSLCLAALGIEPVHQVSEGRFEGGDFMPAGEFALVGCGSRTNPEGVRDL
                     LGGGLGFDEVGVVSEPRHPLISGRDSMVNMHLDTYLN"
     gene            887..1822
                     /gene="cas1"
                     /locus_tag="VKO45_03290"
     CDS             887..1822
                     /gene="cas1"
                     /locus_tag="VKO45_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HMA04929.1"
                     /translation="MAFQNVPWITVSGFGAHIKSTPTQLVIQRKGEVENYPLRSVRHL
                     VIAGGHTIHTAAITNLLKAGGAISFLDQDSNPVGFLHPPLRHPDEEVKAAQKLAPAHR
                     YALALATGAIGARMARLEVIGEASGGEILYKGELEFLHTSLREMEFLVKLDEVRRLFS
                     LATDMYYEILSRTLPPDLGFRRRTRRPHQDPVNAMLSFGYAMLFSQCMRAVTGANLDP
                     DLGVLHEGTGSLVYDLIEPLKPAMVDDAVFEIARKGLHDGDFEYGERRCHLSEGLIGR
                     LLQKLFISVDAEEIDGLVARFRDSLLSNRPFSVAG"
     gene            complement(1840..2118)
                     /locus_tag="VKO45_03295"
     CDS             complement(1840..2118)
                     /locus_tag="VKO45_03295"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HMA04930.1"
                     /translation="MKTHELYVSGRVQSVGFRACVKKVAGNLGILGEVMNLPDGRVRI
                     TATAEEVILEKFVSMLYGCPRAVIRNLEVRERDLVTFTDFAIKRASER"
     gene            complement(2115..5696)
                     /locus_tag="VKO45_03300"
     CDS             complement(2115..5696)
                     /locus_tag="VKO45_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04931.1"
                     /translation="MPASPNLSHIRKKIHQALLHKDLDGIRSILEDVRTIPKRKERRT
                     ILSVIHEAIKESIFKDEIPDFLIFLGDLPFPEIQEVADRAIKAYIASRNERWLQKTVE
                     LCHSLDRKNYQSKIFSVVSKDLIEAGVAQKDRQLMERGMSTIHLISFRKHRSSIMMDI
                     IPMIIVWSVSTEDDRHLYTSLQLIDVIGDVSKRSILHSEIAKAIGMIGILQRDIRGVR
                     EAVKIAIIIKQKQRRIGCISHILERGWSSPVGKQLEDVVGFMNLIGEVPAPRKYEILA
                     VLIEHLLNRVKDKKQINYTLQNLVEKIPVSRECIVLKLVKKAEASGDKWYFERALEFN
                     EQLKDATRIPIKEIIASSSSVVERTGDVSLLMKTVPLLDAVAKTSEESFLPQYLMVAD
                     TLLKRGNLQNAVEIFARIGGPLESANKVAMDTCVRILREGIFRDETELLKEKILSPMD
                     GETRDFVVNRAVTEFCTDAEFGDIIAHLPSLQSLTLAHRTPGVIIPECIRILIERNFI
                     EFQDPSALLSLANQIPNRAEKEQIVSAIVIGIAKVGVAHKNRDLLQRAVGLSCEIKEQ
                     RPRSEALGHIIDQATLLAVEQGDLDLLHRMMKWTGSLLERDFKLYAIDSVIKGMITYG
                     IHHQSPKALDEAYLITHTVDDPSLQQQQREAIIEGLVRVGCIRLTGAVTPITPEALKF
                     EIEPFDRALTLLIESVNKSQLSLKISKFIDIILEYSQAANRQEFLIPLTMFILNIEDR
                     LERNAMFYRISGNFRDVMEDLESTDPYEIVAAVLQRLQSGNISPLVLDLIHRLLEQTP
                     DSYKKFAGLCNLADSYYSIQERDKGTEILKKLYSNLSSLTDPYERARILADLAGMLAR
                     VDEALARDCLEAAIALLNQVKDEKGSLLRKHIVFAILSLHTLKTQEENLETALDLVKQ
                     IEDPTEYVNALIAILSMVITGEKAKEILANIYDAIAAIPSTYERVAMLLDVVPLAEQY
                     GDPGDAPRLLDEARAEVDSISIPYIATMIKKGLVQVMLMVYHKRSDEAIRRKAKEVTL
                     RIEDEETRSQLLSQVGYDRAEAGVSPVFTSLHQLREKIKEGRNPLNELSSITHQINTL
                     PDRARRAAYYIDLFRIFTESKEEKMAERMLSAAIDEAAIIRPLSRRAYVLGDLALNVY
                     NAGFEERSKDILGMAVYAAMNIRDEGLRDEVFYELDVALRIIEERLA"
     gene            complement(5970..7334)
                     /gene="glmM"
                     /locus_tag="VKO45_03305"
     CDS             complement(5970..7334)
                     /gene="glmM"
                     /locus_tag="VKO45_03305"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HMA04932.1"
                     /translation="MSEEKPGKRLFGTNGVRGVVNEDMSPELALGIGSALGSMRRGRI
                     AVGRDTRTSGPALAGALKAGILATGCDVVDLGVLPTPALQYIVRGGFAAGAMVTASHN
                     PPEYNGIKVIESDGTEMGDAEVIQLEDRLFARRFELAPWDRIGRQSSAPDRVGEYLAG
                     VTGQFPAGEGTGMTVVLDPGSGAGSRTTPEILTRMGCRVHTLNAAEDGTFPNRPPEPS
                     PEGLRPLALMVTATGAAMGMAHDGDADRTVFIDEKGRYLEENREFALVARSLCAEKKG
                     VVVAPVSTSLLVEEVAAGLGCRVEYTPVGSIYVARRMLDLIARGTPVVMGGEGNGGLI
                     YPGHQFCRDGAMTAAKMVSIVSRSGKPLSELADSLPRYHMFKEKFRTGDARTLVGCLV
                     KRFAAEKPDLTDGMRLNREDSWALVRASGTEPLVRVTVEARGEREAKDLLKEVQEAMT
                     TCGG"
     gene            complement(7331..8038)
                     /locus_tag="VKO45_03310"
     CDS             complement(7331..8038)
                     /locus_tag="VKO45_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5806 family protein"
                     /protein_id="HMA04933.1"
                     /translation="MQEPEGAGDAQKYQKFKKVDGATYEKVNTFLRKRTYVTAREWAI
                     ARLCSDFKTTGGSEMTFIGEHLPELVPFMTGTYTPQAVNQARSAFKRKVKKAGATFFY
                     GAMCGFFTAEELDDILFEASEVAKFLLEVEGTSLDIDEEISIEDRITEVMRNVAVSAA
                     KLRRERETVGDQVTGDEPVPPEPDSGRSGGDEETDASGPGKNRGESSPDGEQDRVRAE
                     DPGPQGVHGDPGPGDEP"
     gene            8253..9173
                     /locus_tag="VKO45_03315"
     CDS             8253..9173
                     /locus_tag="VKO45_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04934.1"
                     /translation="MIQGLPRRRLRPIVLALLILVSVAGMVRSQGTSSIIYSTDFSSD
                     PDWVTNNPYTNHWDSARGVFHYALRDSTNTFVYKRIPYSGDSFRLEYDLFPLKTDFQA
                     SFRLGLGNREMTINQGTTIFTEFENGPYGNIIWLHIIDQSNQRREISSYGQSYGGPSV
                     RFSDGTPYHVILEYSHERKSATITVNFLFNGSNLWGYSLSDISNLGVMDRIYLSTIGD
                     FENPSAVAEGDIDNVEMRLLTPSEETTIPSTTTSSPTTVTGGTTSTTRTFPYTTTLPP
                     TTTQRSSPIPILILLGALFGASLLYLARRR"
     gene            complement(9197..10390)
                     /locus_tag="VKO45_03320"
     CDS             complement(9197..10390)
                     /locus_tag="VKO45_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HMA04935.1"
                     /translation="MEGKVPLGELEGRLGRFRARMEKDSPGWEMAAIFGRINLYYLTG
                     TIQDGVLLIPAEGDPVFWVRRSYERARDESSFPWIRPMSGFRDAARETGGIPGRIHLE
                     TELVPLALLERFRKYFPVAEVCSLDRQILRVRSVKSPFEQSLMRRAGAIHESVLEERV
                     PEILRPGITEAALACELYSVMVGEGHQGIVRFGSFNTEIEVGQVGFGENSLYPTCFDG
                     PGGCTGLSPAAPVLGSRKRKLKRGDLVFIDNACGVDGYQTDKTMVYMFGRPPPDEVIE
                     IHRRCVEIQDRMAGLLLPGSSPSSIYAGVVENLEPDFLENFMGFGNRRASFLGHGVGL
                     VVDEPPVIAPGFDEPLEEGMTIALEPKKGIPGTGMVGIENTFLVTNGGGVSLTGTSPG
                     LIPVP"
BASE COUNT         1819 a         3442 c         3315 g         2055 t
ORIGIN      
        1 attcaggtag gtgtccagat gcatgttcac catggagtcc cgtccgctga tgaggggatg
       61 ccggggttcg ctcacgacgc cgacctcgtc aaagccgagc cctcccccga ggagatcccg
      121 gacaccttcc gggttcgtcc gcgatccgca gcccacgaga gcgaactccc ccgcaggcat
      181 gaagtcccct ccctcgaacc gcccctcact cacctggtgg accggctcga ttcccagggc
      241 ggcaaggcag agggaggata cccccacctc ctccgttcgc tcgggggtgg ccatccttcc
      301 caggcagatc ccccggtcgg tggccgcctg ctgatcccgc atgaagtaga ggttgtggag
      361 gggggagcgg agccgcaccc cgcccgcccg gccatgcccg gtgtccgggt tcaggacgat
      421 gatccggaag aggtgctccg ggtcccgctc ctccagggga acggcggggg ggcccctgcc
      481 gccgccgagc ctcctcgccg cgagggaaac gaggaggtcc cggacggggc ccggctcccc
      541 ggcctcctcg aggagggtgg ccttgaggtg gtggatcctg accccgaagt tgttgtggag
      601 aaggtcacaa agctggcggt gctcccaccg ggcctcgcgg tggttgaaga aacgctcgta
      661 caggctggcc tccggggcaa ggagggcgaa aaagacctcg atgcccggtt cgtggaccat
      721 cacgtgcctg agagggtccc actccgcctg cgcccccggt ttcatccacc ggatctaggg
      781 ttcccgcgga tgaagagggt ttctctcccg gcccgggaag aaacgatgag ttaatgaacc
      841 gcgaatctaa ccttcttccg tcgattccat ttcctgcagg attacaatgg ccttccagaa
      901 tgtcccctgg atcaccgtgt cgggattcgg ggcgcacatc aagtccaccc ccacccagct
      961 cgtgatccag agaaagggcg aggtcgagaa ctatccgtta cggagcgtcc gccacctggt
     1021 catcgccgga gggcacacca tccacaccgc ggccatcacg aacctcctca aggccggggg
     1081 agccatcagc ttcctcgacc aggacagtaa ccccgtagga ttcctgcacc cccccctgcg
     1141 ccatcccgac gaggaggtga aggccgccca gaagctcgcc cccgctcacc ggtacgccct
     1201 tgccctcgcc accggggcta tcggggcgag gatggcccgg cttgaggtga tcggggaggc
     1261 ctcgggcggc gagattctct acaaggggga gctcgagttc ctccacacct ccctcaggga
     1321 gatggagttc ctcgtgaagc tcgacgaggt ccggaggctc ttctccctcg cgaccgatat
     1381 gtattacgag atcctctccc gcaccctccc ccccgatctc ggcttccgac ggcggacgag
     1441 gaggccccac caggacccgg tgaacgccat gctctccttc gggtatgcca tgctcttctc
     1501 ccagtgcatg cgggcggtca ccggcgcgaa cctggacccg gatctcgggg tgcttcacga
     1561 gggcacgggg agcctcgtgt acgacctcat cgagcccctc aagccggcga tggtggacga
     1621 cgcggtcttc gagatcgccc ggaaggggct tcacgacggc gatttcgagt acggggaaag
     1681 gcggtgccac ctctccgagg ggctcatcgg ccggctcctc cagaagctct tcatctcggt
     1741 ggacgcggag gagatcgacg gcctcgtcgc ccggttccgg gattccctcc tctccaaccg
     1801 ccccttctcg gtcgccggct gaaagagaga gggctttttt cagcgttcgg aggctctttt
     1861 aatggcgaag tccgtgaagg tgacgagatc ccgctccctg acctccaggt tccggatgac
     1921 cgcccggggg cagccataga gcatggagac gaacttctcg aggatgacct cctcggcggt
     1981 cgcggtgatc cggaccctcc cgtccgggag gttcatcacc tcaccgagga tcccgaggtt
     2041 tccggccacc ttcttcacgc aggcccggaa cccgacgctc tggacgcggc cggagacgta
     2101 gagctcgtgg gtcttcacgc aaggcgctcc tcgatgatcc ggagggcgac gtcgagctcg
     2161 tagaagacct cgtccctgag gccctcgtcc cggatgttca tggcggcgta gaccgccatc
     2221 ccgaggatgt ccttggaccg ctcctcgaaa ccggcgttgt agacgttgag ggcgagatcc
     2281 ccgaggacat aggccctccg ggagaggggc cggatgatgg cggcctcgtc gatggcggcc
     2341 gagagcatgc gctcggccat cttctcctcc ttgctctcgg tgaagatccg gaagagatcg
     2401 atgtagtacg cggcccgcct cgcccggtcc gggagggtgt tgatctggtg ggtgatgctc
     2461 gagagctcgt tcaggggatt ccgcccctcc ttgatcttct cccgcagctg gtggagggag
     2521 gtgaagaccg ggctcacccc cgcctcggcc cggtcatagc ccacctggga caggagctgg
     2581 gaccgggtct cctcgtcctc gatgcggagg gtgacctcct tggccttccg ccggatggcc
     2641 tcgtcgctcc tcttgtggta caccatgagc atcacctgga cgaggccctt cttgatcatg
     2701 gtggcgatgt acgggatgga gatggaatcc acctcggccc gcgcctcgtc gaggagccgg
     2761 ggagcgtcgc cggggtcgcc gtactgctcg gccaggggga ccacgtcgag gagcatggcc
     2821 acccgctcgt acgtggaggg gatggccgcg atggcgtcgt agatgttcgc gaggatctcc
     2881 ttcgccttct cgccggtgat caccatggag aggatggcga tgagggcgtt cacgtactcg
     2941 gtcgggtcct cgatctgctt cacgaggtcg agggccgtct ccaggttctc ctcctgggtt
     3001 ttcaacgtgt gaagggagag gatggcgaag acgatgtgct tcctgaggag cgagcccttc
     3061 tcgtccttca cctggttcaa cagggcgatg gcagcctcca ggcagtcccg ggccagggcc
     3121 tcgtccaccc gggcgagcat gcccgcgaga tcggcgagga tcctcgccct ctcgtaagga
     3181 tcggtgaggg aggagaggtt gctgtagagc ttcttcagga tctcggtccc cttgtcccgc
     3241 tcctggatgg agtagtacga gtccgcgagg ttgcagagcc cggcgaactt cttgtacgag
     3301 tccggcgtct gctctagcag gcggtggatg aggtcgagga cgagaggcga aatgttcccg
     3361 ctctggaggc gctggaggac cgcggcgacg atctcgtagg ggtcggtgga ctcgaggtcc
     3421 tccatcacgt cccggaagtt ccccgagatc cggtagaaca tggcgttccg ctccagccgg
     3481 tcctcgatgt ttaggatgaa catcgtgagc gggatgagga actcctgccg gttcgccgcc
     3541 tgggaatact cgaggatgat gtcgatgaac ttcgagatct tcagggagag ctgggacttg
     3601 ttcacgctct cgatgaggag ggtgagggcc cggtcaaagg gctcgatctc gaacttcagg
     3661 gcctccgggg tgatgggggt gaccgcgccc gtgagccgga tgcacccgac ccggacaagc
     3721 ccctcgatga tggcctcccg ctgctgctgc tggagcgacg ggtcgtccac cgtgtgggtg
     3781 atgaggtacg cctcgtcgag ggccttgggg gactggtggt ggatgccgta ggtgatcatc
     3841 cccttgatca cgctgtcgat ggcatagagc ttgaagtccc gctcgaggag cgaacccgtc
     3901 cacttcatca tccggtggag gaggtcgaga tcgccctgct ccacggcgag aagggtggcc
     3961 tggtcgatga tgtgcccgag ggcctccgac cggggccgct gctccttgat ctcgcaggag
     4021 agcccgaccg cccgctggag gaggtcccgg ttcttgtgag ccaccccgac cttcgcgatc
     4081 ccgatgacga tggccgagac gatctgctcc ttctcggccc ggttcgggat ctggtttgcg
     4141 agggagagga gggccgaggg gtcctggaac tcgatgaagt tccgctcgat gaggatccgg
     4201 atgcattcgg ggatgatgac ccccggcgtg cggtgggcga gggtgaggga ctggagggac
     4261 gggaggtggg cgatgatatc cccgaactcc gcgtcggtgc agaactcggt caccgcccgg
     4321 ttgacgacga agtcccgggt ctcgccgtcc atgggcgaga ggatcttctc cttcaggagc
     4381 tcggtctcat cccggaagat accctccctg aggatccgga cgcaggtgtc catagccacc
     4441 ttgttcgcgc tctccagggg gccgccgatc cgggcgaaga tctccaccgc gttctggagg
     4501 ttgccccgct tgaggagggt atcggcgacc atgaggtact gggggaggaa ggactcctcg
     4561 gaggtcttgg ccaccgcgtc gaggaggggg acggtcttca tgaggaggct cacgtccccg
     4621 gtccgctcca cgacggagct cgaggaggcg atgatctcct tgatggggat ccgggtggca
     4681 tccttcagct gctcgttgaa ctcgagggcc cgttcgaagt accacttgtc tcctgacgcc
     4741 tcggccttct tcacgagctt gaggacgatg cactccctcg acacggggat cttctccacc
     4801 aggttctgga gggtgtagtt gatctgcttc ttgtccttca cccggttcag gaggtgctcg
     4861 atgaggacgg cgaggatctc gtacttcctc ggggcgggga cctccccgat gaggttcatg
     4921 aagccgacca cgtcctcgag ctgctttccc acgggggatg accatccccg ttcgaggatg
     4981 tgggagatgc agccgatgcg gcgctgcttc tgcttgatga tgatggcgat cttcaccgcc
     5041 tcgcggaccc cccggatgtc ccgctggagg atcccgatca tgccgatggc ctttgctatc
     5101 tccgagtgga ggatggagcg cttggagacg tccccgatga catcgatgag ctggagggag
     5161 gtgtagaggt gccggtcgtc ctcggtggag acgctccaga cgatgatcat ggggatgatg
     5221 tccatcatga tgctcgaccg gtgcttccgg aagctgatga ggtggatggt ggacatcccc
     5281 cgttccatga gctgccggtc cttctgcgcg acacctgcct cgatgagatc cttcgaaacg
     5341 accgagaaga tcttggactg gtagttcttc cggtcgaggc tgtggcagag ctcgacggtc
     5401 ttctggagcc accgctcgtt cctcgacgcg atgtaggcct tgatggcccg gtccgccacc
     5461 tcctggatct ccgggaaggg gagatccccg aggaagatca ggaagtcggg gatctcgtcc
     5521 ttgaagatgc tctccttgat ggcctcgtgg atgacggaga ggatggtccg ccgctccttc
     5581 ctcttgggga tggtccggac atcctcgagg atggaccgga tgccatcgag atctttatgt
     5641 aggagggcct ggtggatctt cttccggata tgggataaat tggggctggc cggcacggaa
     5701 atcccgcact acatgatatg acctgatttt atttataaag atttcgatta aaaactattc
     5761 attaaacgca tcagaactcg tatttatcaa atattacaaa agattacggc tttatggccc
     5821 tttcaagagt aatagaaacc aattgatgaa ttctgaccga tattttcccg tgaagcgagc
     5881 gcatttttaa tatttacagc cagcatttcc ccttcccggg gacccatcat ataaaatgag
     5941 gaaaataatc tggctttcct ggcatctggc tatccaccgc aggttgtcat ggcttcctga
     6001 acttctttca ggagatcctt cgcctccctc tctccccgcg cctccaccgt gaccctgacg
     6061 aggggttcgg tccccgaggc acggacgagg gcccaggaat cctcccggtt caggcgcatc
     6121 ccgtcggtca gatcaggctt ctcggcggcg aacctcttta ccaggcatcc gaccagggtc
     6181 cgggcatctc cggtccggaa tttctccttg aacatgtggt accttgggag ggagtccgcg
     6241 agctccgaga ggggtttacc cgatcgggag acgatggaga ccatcttcgc ggccgtcatg
     6301 gcaccgtccc ggcagaactg gtggcccggg tagatcagcc cgccgttccc ctccccgccc
     6361 atgacgaccg gtgtgccccg ggcgatgagg tcgagcatcc gccgcgccac gtagatggac
     6421 ccgaccggcg tgtactccac ccgacacccc aggccggcgg ccacttcctc gacgaggagg
     6481 gaggtgctca ccggggccac caccacgccc ttcttctccg cgcacaggga ccgggcaacg
     6541 agggcgaact cccggttttc ctccaggtag cgccccttct cgtcgatgaa gaccgtccgg
     6601 tcggcgtcgc cgtcatgggc catgcccatc gcggcgccgg tcgcggtcac catgagggcg
     6661 aggggccgga gcccctcggg ggagggctcc ggggggcggt tggggaaggt gccatcctcg
     6721 gcggcgttca gggtgtggac ccggcacccc atccgggtga ggatctccgg ggtcgtccgg
     6781 gaacccgcgc ccgagcccgg gtccaggacc acggtcatcc cggttccctc accggccggg
     6841 aattgcccgg tcaccccggc caggtattcc ccgacccgat ccggtgcgga ggactgcctt
     6901 ccgatcctgt cccacggggc cagctcgaac cggcgggcga acaggcgatc ctcgagctgg
     6961 atcacctcgg catcccccat ctccgttccg tcgctctcga tcaccttgat cccgttgtac
     7021 tcggggggat tatgggaggc ggttaccatg gctcccgccg caaacccgcc ccggacgatg
     7081 tactggaggg cgggagtcgg gagaaccccg aggtccacca cgtcgcagcc ggtggcgagg
     7141 attcccgcct tgagggcgcc cgcaagggcg ggcccggagg tcctcgtgtc ccggcctacc
     7201 gcgattcgcc cccggcgcat cgatccgaga gcggacccga tcccgagggc gagctccggg
     7261 ctcatgtcct cgttgaccac gccacggacg ccattcgtgc cgaagaggcg cttcccgggc
     7321 ttttcttcac tcacggttca tcccccgggc ccggatcccc gtgtaccccc tgcgggcccg
     7381 gatcctccgc tctcacccgg tcctgttccc catccggtga gctctctccc cggttcttcc
     7441 cgggcccgga ggcatccgtt tcctcgtctc ccccggacct gcctgaatcc ggctccgggg
     7501 ggaccggctc atccccggtg acctggtccc cgactgtctc ccgttccctt cgcagcttcg
     7561 cggccgatac cgccacgttc ctcatgacct cggtgatccg gtcctcgatg gagatctcct
     7621 cgtcgatgtc caggctggtt ccctcgacct cgaggaggaa cttcgccacc tcgctcgcct
     7681 cgaagaggat gtcgtccagc tcctcggcgg tgaaaaagcc gcacatggcc ccgtagaaga
     7741 aggtggcccc ggccttcttc accttccgtt taaacgcgct cctcgcctgg tttaccgcct
     7801 ggggggtgta ggtgcccgtc atgaagggaa cgagctcggg gaggtgctcg ccgatgaagg
     7861 tcatctccga gccgcccgtg gtcttgaaat cggagcagag ccgggcgatg gcccactccc
     7921 gtgcggtgac ataggtcctc ttccggagga aggtgttcac cttctcgtag gtggccccgt
     7981 ccaccttctt gaatttctgg tatttctgcg cgtcgccggc tccctcaggc tcctgcattc
     8041 actgtactcc tgagaaggtg caggtaatga aggtgatgag tcccgcggga ggggcatatg
     8101 tagagcatcc cgcggtttat gacaccccgg ggagcagggg ccgggaccct ggatcaggta
     8161 gtatacatat aaatgagcag gtacacctgt actgagattc atctggggtc gtcccggaaa
     8221 acgggagacc cggaaatgaa ggagtcccga ccatgatcca gggattgccc cggcggcgtc
     8281 tccgccccat cgtccttgcc ctcctcatcc tcgtatcggt ggcgggcatg gtccggtccc
     8341 aggggacctc gagtatcatc tactccaccg acttttcctc ggatcccgac tgggtcacca
     8401 acaacccgta cacgaaccac tgggattcgg cgaggggcgt gttccactac gccctccggg
     8461 actccacgaa cacctttgtg tacaagagga tcccctattc gggggactcc ttccggctcg
     8521 aatatgatct cttccccctg aaaaccgatt tccaggcatc cttccggctg gggctcggca
     8581 accgtgagat gaccatcaac caggggacga ccatcttcac cgagttcgag aacgggccgt
     8641 acgggaatat catctggctc catatcatcg accagtcgaa ccagaggagg gagatctcgt
     8701 cctacggcca gtcctacgga ggaccctcgg tacgcttctc cgacggtacc ccgtaccatg
     8761 ttatcctcga gtacagccat gagcggaagt ccgcgaccat caccgtcaat ttcctgttca
     8821 acgggagtaa cctctggggg tattccctct cggacatctc gaacctcggg gtcatggacc
     8881 ggatctatct ctctaccatc ggggatttcg agaacccgag tgcggtggcc gagggtgaca
     8941 tcgacaacgt cgagatgcgc ctcctgacgc cctcggagga aaccaccatc ccgtccacca
     9001 ccacctcgtc cccgacgacc gtgaccggag ggacgacctc gacgacccgg accttcccgt
     9061 acacgaccac gctccccccg accaccaccc agagaagctc cccgatcccg atcctcatcc
     9121 tcctcggagc cctgttcggg gcatccctgc tgtatctcgc ccggagaagg taaggaatcc
     9181 gaaacggccg acggtctcac ggaaccggta tcaggcctgg actcgtcccg gtgagggaga
     9241 ctcccccgcc attcgtgacg aggaaggtat tctcgatgcc caccatcccg gttcccggga
     9301 tccctttctt gggttcgagg gcgatggtca tcccttcctc cagaggctcg tcgaaccccg
     9361 gtgcgatcac cggaggttcg tccacgacga gcccaacccc gtggcccagg aagctcgccc
     9421 gccggttccc gaatcccatg aagttctcga gaaaatccgg ctccaaattc tccactaccc
     9481 cggcgtagat cgatgatggc gaactgccgg ggaggagcag cccggccatc cggtcctgaa
     9541 tctctacgca acgccggtgg atctctatca cctcatccgg agggggcctc ccgaacatgt
     9601 acaccatggt cttgtccgtc tggtaaccgt cgaccccgca ggcgttgtcg atgaacacga
     9661 ggtccccgcg cttcagtttc cgcttccggc tgccgaggac cggcgcggcg ggactgagcc
     9721 cggtacagcc tccggggccg tcaaagcagg tcggatacag ggaattttcg ccgaacccga
     9781 cctggcccac ctcgatctcg gtgttgaagc tcccgaaccg gacgattccc tggtgtccct
     9841 ccccgaccat gaccgagtag agctcgcatg ccagcgccgc ttcggttatc cccggcctga
     9901 ggatctccgg cacacgctct tcgagcacac tctcgtgaat ggcccccgcc cgtctcatga
     9961 gggattgttc gaaagggctc ttcaccgacc tgacccgcag gatctgcctg tccagggagc
    10021 atacctcggc gacggggaag tatttcctga atcgctcgag gagggcgagg ggcacgagct
    10081 cggtctccag gtgtatccgg ccggggatcc cgccagtttc ccgggccgcg tcgcggaacc
    10141 cggacatggg cctgatccag ggaaaggagg attcgtcccg cgccctctcg tagctccggc
    10201 gcacccagaa gacgggatcc ccctcggccg ggatcaggag caccccgtcc tggatggttc
    10261 ccgtaaggta gtacaggttg atcctgccga aaatcgcggc catttcccag cccgggctgt
    10321 ccttctccat ccgggcccgg aaacgcccga ggcgtccttc gagttcaccg agtggaactt
    10381 ttccctccat ggccctcccc cgataagcag gatacatctc gcctcccttc tgccgtgaga
    10441 atcgacgccg gagcgcttaa attctccatc caggaccggt tcgaacctcc ccggtctgcc
    10501 cggattcacc ccgcatatgt atagcaacat acggtttatc ggagcccacg ggattgctgg
    10561 cggggtattc cagagcggag gcaatcgggg tggaaatccg gatccggccc attattgtgg
    10621 attttctcct t
//
LOCUS       DATGDI010000089        12461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_386530, whole genome shotgun sequence.
ACCESSION   DATGDI010000089 DATGDI010000000
VERSION     DATGDI010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 12461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12461
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386530"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VKO45_03325"
     CDS             complement(<1..375)
                     /locus_tag="VKO45_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HMA04936.1"
                     /translation="MELIERILKAGASNAQEIEVYLVEGSSVSAELKREKVATATGSR
                     DWGLSIRTIARGQIGASATSSPARWEECLEAALASMRLATPQEWKGLPEPAELRKDAP
                     SFDKRVSPEPPAAQDLLRGMLEG"
     gene            complement(362..1699)
                     /locus_tag="VKO45_03330"
     CDS             complement(362..1699)
                     /locus_tag="VKO45_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HMA04937.1"
                     /translation="MPPPGIRFYDVRCVRATATHVDVDNGVVESAGTSFSDSALVRVL
                     GPLGWGMISVNNLDLSGKKELDRILSEGLALARITEDPVDLAEPPRGEHPVPAPREDP
                     RSLALEEKTTVLSGIEKAAKLPGIASTRATYIERTEEVFYTDSSGFEHRSTVTRSGFS
                     VLAVARRNGLAQMGRESHHTIHGFNLRHQEQTGAKAGETALALLNAGTAKGGRMRAVL
                     DPELAGVFAHEAVGHASEGDLVKEGSSVLAGRIGERIGPDLLTIVDDPSLPEFGFEPV
                     DAEGCRTGRTEMISRGRVNAYLHTRETLAALGNGMAGHGRAEGGEPPLVRMSNTFIEE
                     GDATLDEILAECRDGILLVGSRGGQVDPGRGVFQFNAEYGYLIERGEKGRMVRDVSLS
                     GEILSTLHGILLCGKDRAMHQGFCGKGGQSVPVSDGSPHILLADAVVGGSGTD"
     gene            complement(1699..3648)
                     /gene="lonB"
                     /locus_tag="VKO45_03335"
     CDS             complement(1699..3648)
                     /gene="lonB"
                     /locus_tag="VKO45_03335"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HMA04938.1"
                     /translation="MEPTDTQKLPDEEQVLGGLELAVSSEIEVPPKLIDQVIGQDHAV
                     EVIRKAAIQRRHVMMIGSPGTGKSMLAKAMAELLPREELQDILVYPNSEDSNNPIIRT
                     VAAGRGKQIVAAHKAEAKRKMQTRNTLILLFVFGIVAFAFITYQWLMGIIAAAFVFMA
                     LRYTTPREDLLVPKLLVSNDATATAPFIDATGSHAGALLGDVRHDPFQSGGLETPAHD
                     RVEAGAIHRAHGGVLFIDEINSLEPASQQSLLTSLQEGIFPITGQSERSSGAMVRTEP
                     VPCKFIMVAAGNVDAVQNMNPALRSRIRGYGYEIFFRDSMDDTPENRRKFVRFIAQEV
                     QKDGKIPHFDRSAIEEVIREGRRRSNRKGHLTLKLRDIGGLIRVAGDLARQKGAKVTT
                     ADDVVKAKETARSIEEQISDDYITRKRDYNLTVVEGARIGQVNGLAVYGEDSGQVQQI
                     MAEVTPAQGESGQIITTGIFERRKEPWLRGENEESIVQQSIKNVSALIKKFTGKDIKN
                     MDVHIQFIGTYGVEGDSASVSVATAVISAIEGIPVHQDMAMTGSLSVRGDVLPVGGVT
                     FKIEAAAKAGIHKVLIPKTNMDDVLIEDRYRGMVEIVPVETIQDVLLNALVAEGRESF
                     LTRIKKMAISAPVKILDSAFPNPAI"
     gene            3746..4591
                     /gene="prs"
                     /locus_tag="VKO45_03340"
     CDS             3746..4591
                     /gene="prs"
                     /locus_tag="VKO45_03340"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HMA04939.1"
                     /translation="MKVACTGHSRALGARIAGHLGAGIAETRFSRFPDGEIYLQTGPL
                     DETTVVVGSLLSGDSLVELLLLTDACQRSEVLLVVPYLGYARQDREFKPGEPVSARAI
                     ARALSIGVSRVITVNAHKETVLTHFTVPASQVNLAPEVGDYIRGLGLSDPLILAPDKG
                     AAPFAAAVASRGGWETDHLEKTRISGTEVRVEPRRGEAWTGDVVLVDDIISTGGTLAT
                     AARMLRAQGARGVHAVCVHGLFAGSALGLLRSAGIDLIAASDTIEGPESRYSAAGPIA
                     RALSR"
     gene            4588..5571
                     /gene="rtcA"
                     /locus_tag="VKO45_03345"
     CDS             4588..5571
                     /gene="rtcA"
                     /locus_tag="VKO45_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HMA04940.1"
                     /translation="MITIDGSQLEGGGQILRGAVALSALSRTPVEVMRIRARRGKPGL
                     APQHIAAVRAVAAACDATCDGLRVGSDRIVFHPGLPVAREVRADVGTAGSVALVLQAW
                     LPVALVAGGSITVTGGTEVDRSPTIDYLEQVLFRVLARHGAKITTEILKRGYYPEGGG
                     EVRVSVEPCRLAPLALSDEKPGSCGIISCSANLPDHVAERQAGAARRLLEGRLKIPCE
                     VRLDRRRGASTGTSCTVWAGAKGGISLGKRGVPADRVGEMAARALLDEVGRGGQVDTH
                     LADQLLVFLALSGGRYTAPACSLHAATMCWLLERFGYRVHSRTNGVVEFSA"
     gene            5568..6023
                     /locus_tag="VKO45_03350"
     CDS             5568..6023
                     /locus_tag="VKO45_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HMA04941.1"
                     /translation="MRYAVDASFFFSELVLDGEISTPPSIVEELADARSRCRLEALLA
                     AGLSVTPPSPPSLKRVAGAAAETGDTGRLSPADTDLLALSLDLGATVVTDDYAVQNVA
                     IRLGLGVRSILQKRARSRQWRFRCPGCNRRYPAAGTCPACGTVLKRSLK"
     gene            6047..6655
                     /locus_tag="VKO45_03355"
     CDS             6047..6655
                     /locus_tag="VKO45_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase-like protein"
                     /protein_id="HMA04942.1"
                     /translation="MSSLDDLIRRARMLHAEGHSQSQISDELNLSMETVTYLLMKEKE
                     GGVPRDVLIDWTPVSGRADLLDDLAALMIGRSRSPGEGPGGEGGEPTAVVGIALSGVP
                     LATLIALQECLKLAVYFPSRHSTADPPVGSLSGNFAPVNGEGCILVDDVITSGKTLGE
                     AVDYLRGHGANPLAIWVIFDKRGLREIDGVPVLALFRVSRLD"
     gene            6722..8335
                     /gene="thsA"
                     /locus_tag="VKO45_03360"
     CDS             6722..8335
                     /gene="thsA"
                     /locus_tag="VKO45_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HMA04943.1"
                     /translation="MLAGQPVIILRDNVERTRGHDAQRSNIAAAKAIAAAVRTTLGPR
                     GMDKMIISSTGDIVITNDGATILHELSIQHPGGKMVVEVAETQDDEVGDGTTTACILV
                     GALMEEAEWMLEQEIHPTVIAQGYRMAMEKALEIVKNLAITVKPDDVKTLKIIADTAM
                     TGKSIEQVKEKLDGIVVDAVRAVVQTVDGKLVVDEDDVKIKKHVGETIDDAEFIRGMV
                     VEKKRVSEEMPKRIEKARIALLATPLEITKTQVKSKIKITSPDQLMAFDTQERGALHE
                     MAEQVIKTGANVILCQKGIADSVQYYLAKEGIMAIEDVPEKDMLFAARALNGQIANKP
                     HDLTAKMLGHAELAEEMEGIDLIKISGCRNPRAVTILVRGSTQVLIDEMERAVYDGIR
                     VVMDAIEDQKYVVGGGAVETEVMLRVRDYAASVGGRIQLAIEAFANAFESIPRTLAEN
                     SGFNPIDKLVELKTAHAKGKKRAGLNVYTGKIVDMFDENVFEPMRVKTQAIQSAAEAS
                     NLLIRVDDMMVTQKKEPAMPPAGAMPPMG"
     gene            8449..8533
                     /locus_tag="VKO45_03365"
     tRNA            8449..8533
                     /locus_tag="VKO45_03365"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8483..8485,aa:Ser,seq:cga)
     gene            8750..9595
                     /locus_tag="VKO45_03370"
     CDS             8750..9595
                     /locus_tag="VKO45_03370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HMA04944.1"
                     /translation="MFEQDVVKPGYIFGCILSLLALSWGIPILVAPGFLPGWSLGLIP
                     FLPGILALVFLSLEDHPIQVHARPLFGPVKVPAVILSVAYPFLLLGLAALVALGTGLG
                     NLNPGAGTGIDTALAAGVVAFLVAIPASLLQEYGYRGYLLPALTYWRGKIAATLIVGI
                     LWGLSMAPFSYLVLSGAGAGDPLTLAFLGFLLTVSVAFAFSSCYYLSESILPVTLMNV
                     LLTILVPAFFSAGWNPTGAASGGLIGVTWPSPVPLLLLVALAFVPVIAWLFTVMDGEM
                     FGDEL"
     gene            9713..10657
                     /locus_tag="VKO45_03375"
     CDS             9713..10657
                     /locus_tag="VKO45_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA04945.1"
                     /translation="MSEPESIIEVQGLSHSFGEVVAVDSISFTVRKGEIFSFLGPNGA
                     GKSTTINVLTTLLPIQKGQVRIAGHDVAAEPGEVRRSIGIVFQEETLDRDLTVREILE
                     FHGRLYSMPRDERRARIDEMIRLVELEKKRDVLVKHLSGGMKRRLEIARGLMTRPGVL
                     YLDEPTLGLDPQSRLRVWDYIRQVNREGTTIFLTTHYMDEADSLSDRISIIDHGKIIA
                     SGTSEELKNALGEDMIYLETGDDAKTTGILRNLPEVKDVKPSLRGLSVTLNNEGTKTL
                     PVILDRLKEGGIEIRSANLKKPTLDDVFVHFTGREIRG"
     gene            10670..11506
                     /locus_tag="VKO45_03380"
     CDS             10670..11506
                     /locus_tag="VKO45_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA04946.1"
                     /translation="MATEFVTVYWRDMLRFFRFRALLFSSLVQPALWMAFFGVAFTGT
                     LSQLGAPTTAPPGALAVPYLTFMAAGIMALTTLFTSLFGGISLLFDKLWGLMREILAS
                     PMPRPHILIGISLSGMTKSFIQVLIIMLFGLVIGVQFFVGASPLMVLVSVLGILLFVG
                     VFSLGFLFMSATISMRMESPESLQAVITLLSLPLFFVSNALYPASAFPSFLQAATAVN
                     PLTYVVTGIRYFAIGPDFYALGSHYVYTLMDMGFAFLALVVFALVMFGIAWWTFEKAV
                     VT"
     gene            11508..12287
                     /locus_tag="VKO45_03385"
     CDS             11508..12287
                     /locus_tag="VKO45_03385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HMA04947.1"
                     /translation="MGRVGVKPGKQPGGGGSAPGITILREDFLTTVSIPPGSVDLVVT
                     SPPYNVSIPYATVRDDISYTEYLEFTRRWLAKAFTLMKPDGRMCLNIPLDKNLGGQQS
                     VYADILGLAKACGWRYHATIIWNEQNISRRTAWGSWLSARAPYVIAPVETIAVLYRGT
                     WKKAEPGKSDITRDEFIGWTNGVWTFSGEKRGRANHPAPFPLELPTRCIKLFTYVGDT
                     VLDPFMGSGTTLLACRNLGRTGIGVDIDAAYCEVAKKRLSG"
     gene            complement(12372..12458)
                     /locus_tag="VKO45_03390"
     CDS             complement(12372..12458)
                     /locus_tag="VKO45_03390"
                     /inference="COORDINATES: protein motif:HMM:NF045043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04948.1"
                     /translation="MCGNIFLDVVPDQCPICKAFKKKFKEIT"
BASE COUNT         2050 a         4244 c         3912 g         2255 t
ORIGIN      
        1 accctcgagc atcccccgga ggagatcctg tgccgccgga ggttcgggtg acacccgctt
       61 gtcgaaggag ggagcatcct tcctgagctc ggccggttca gggaggccct tccactcctg
      121 cggcgttgca aggcgcatgg aggcaagggc agcctccagg cactcctccc acctcgccgg
      181 cgaggaggtc gccgacgccc cgatctggcc ccgcgcgata gtccggatgg agaggcccca
      241 gtcccgggat ccggtcgccg tcgcgacctt ttcccgcttc agctcggccg agaccgagct
      301 cccctccacg agatatacct cgatttcctg tgcgttcgag gcgccggcct tgaggatccg
      361 ctcaatcagt tccactgcca cccaccaccg cgtccgcaag gaggatatgc ggagagccgt
      421 cgctcaccgg cactgactgg ccccctttgc cgcagaaccc ctggtgcatc gcccggtcct
      481 tcccgcagag gaggatcccg tggagcgtgg agaggatctc tcccgagagg gagacatccc
      541 gcaccatcct ccctttctca cctctctcga tgagatagcc gtactcggca ttgaactgga
      601 atacaccccg cccgggatcc acctgccctc cccgggaacc gacgaggagg atcccgtccc
      661 ggcactccgc gaggatctcg tcgagggtgg catccccctc ctcgatgaag gtgttcgaca
      721 tccggacgag gggcggctcc cccccctcgg cccggccatg tcccgccatc ccgttcccga
      781 gggcggcgag ggtctccctc gtatggaggt acgcgttcac cctcccccgg ctgatcatct
      841 cggtcctccc cgtccggcac ccctcggcat ccaccggctc gaacccgaac tccggaaggc
      901 tcgggtcgtc tacgatggtg aggagatcgg gaccgatccg ctcgccgatc cttcccgcga
      961 gcaccgagga cccctccttc acgaggtccc cctcgctcgc gtggccgacc gcctcgtggg
     1021 cgaagacccc ggcgagctcc gggtcgagga ccgcccgcat cctcccgccc tttgccgtcc
     1081 ccgcattgag gagggcgagg gcggtctccc cggccttcgc ccccgtctgc tcctggtgcc
     1141 tcaggttaaa gccgtggatg gtgtgatggc tctccctccc catctgggcg agcccgttcc
     1201 tcctcgcgac cgcgaggacc gagaagcccg agcgggtgac ggtcgagcgg tgctcaaacc
     1261 ccgagctgtc ggtgtagaat acctcctcgg tccgctcgat gtacgtcgcc cgggtggagg
     1321 cgatccccgg gagcttcgcg gccttctcga ttcccgagag caccgtggtc ttctcctcga
     1381 gggcgaggga tcggggatcc tcccgcggcg cagggaccgg gtgctcacct ctcgggggtt
     1441 ccgcgagatc gaccggatcc tccgtgatcc gggcgagggc gaggccctcg gagaggatcc
     1501 ggtccagttc tttttttccc gagagatcca ggttattcac cgagatcatg ccccacccga
     1561 gcggcccgag aacacgcacg agggctgaat ccgagaagga ggtaccggcc gactccacga
     1621 ccccgttgtc gacgtccacg tgggtggcgg ttgcccgcac gcaccggaca tcgtagaatc
     1681 ggatcccggg cgggggcatc agatggcggg attcgggaag gccgagtcca ggatctttac
     1741 cggggccgag atcgccatct tcttgatccg ggtgaggaag ctctcgcgcc cctcggctac
     1801 gagggcattc aggagcacgt cctggatggt ctcgacgggt acgatctcga ccatgccccg
     1861 gtaccggtcc tcgataagaa catcgtccat gttggtcttg gggatgagga ccttgtggat
     1921 cccggccttt gccgccgcct cgatcttgaa ggtgaccccg ccgacgggga ggacgtcccc
     1981 gcggaccgag agggacccgg tcatggccat atcctggtgg accgggatgc cctcgatggc
     2041 ggagatgacc gccgtcgcca ccgaaacgga ggccgagtcc ccctctaccc cgtaggtccc
     2101 gatgaactgg atgtggacat ccatgttctt gatgtccttc cccgtgaact tcttgatgag
     2161 ggcgctcacg ttcttgatgg actgctggac gattgactcc tcgttctcgc cccggagcca
     2221 tggctccttc cgcctctcga agatgccggt ggtgatgatc tggccgctct cgccctgggc
     2281 gggggttacc tcggccataa tctgctgaac ctggccgctg tcctccccgt aaacggcgag
     2341 gccgttcacc tggccgatcc gggctccctc gaccaccgtc aggttgtagt cccgcttccg
     2401 ggtgatgtag tcgtccgaga tctgctcctc gatggaccgg gcggtctcct tcgccttcac
     2461 cacgtcgtca gcggtggtga ccttcgcccc cttctgccgg gcgaggtcgc cggccacccg
     2521 gatcagcccg ccgatatccc ggagcttgag ggtgaggtgc cccttccggt tcgaccggcg
     2581 cctgccctcc cggatgacct cctcgatggc cgaccggtcg aagtggggga tcttcccatc
     2641 cttctgcacc tcctgggcga tgaaccggac gaacttcctc cggttctcgg gcgtgtcatc
     2701 catggagtcc cggaagaaga tctcgtaccc gtatcccctg atacgggacc ggagggccgg
     2761 gttcatgttc tggacggcat ccacgttccc tgccgcaacc atgatgaact tgcaggggac
     2821 gggctctgtc cggaccatgg ccccgctcga acgctcggac tggcccgtga tggggaagat
     2881 cccctcctgc agggaggtga ggaggctctg ctgggacgcg ggctcgaggg agttgatctc
     2941 gtcgatgaag aggaccccgc cgtgggcccg gtggatggcc cccgcctcga cccggtcatg
     3001 ggcaggggtc tcgaggccac cgctctggaa cgggtcgtgc ctgacatccc cgaggagcgc
     3061 cccggcatgg gacccggtgg catcgatgaa gggggcggtg gccgtggcat cgttcgagac
     3121 gagcagcttc gggaccagga gatcctcccg cggggtggtg taccggaggg ccatgaagac
     3181 gaaggccgcg gcgatgatcc ccatgagcca ctggtacgtg atgaaggcaa aggccacgat
     3241 cccgaagacg aagaggagga tgagggtgtt ccgggtctgc atcttccgct ttgcctccgc
     3301 cttgtgggcc gcgacgatct gcttccccct ccccgcggcc accgtgcgga tgatgggatt
     3361 gttcgagtcc tcggagttcg ggtacacgag gatgtcctgc agctcctccc gggggagaag
     3421 ctcggccatg gccttcgcga gcatcgactt cccggtgccc ggggagccga tcatcatcac
     3481 gtgcctccgc tggatggccg ccttccggat gacctccacg gcatggtcct ggccgatgac
     3541 ctggtcgatg agcttcggag ggacctcgat ctcggaggat accgcgagct cgagccctcc
     3601 gagcacctgt tcctcgtctg gtaatttctg ggtatccgta ggttccattc cctgggtgcc
     3661 cctctcttcg gcacgattaa gtcagtaagt agatataatt ctgttcctga tcgtttaagt
     3721 actttcagct gatggtgatt gacgaatgaa agtcgcgtgt acagggcact cccgggccct
     3781 cggggcgagg attgccgggc acctgggagc cgggatcgcg gagacccggt tctcccggtt
     3841 ccccgacggg gagatctacc tgcagacggg gcccctcgac gagaccaccg tcgtggtcgg
     3901 gagcctcctc tccggcgatt ccctggtgga gctcctcctc ctcaccgatg cctgccagcg
     3961 ttcggaggtg ctcctcgtgg tcccgtacct gggatacgcc cggcaggacc gcgagttcaa
     4021 gcccggtgag ccggtctcgg cccgggccat cgcccgggcc ctctccatcg gcgtctcccg
     4081 ggtcatcacc gtgaacgccc acaaggagac cgtcctcacc cacttcaccg ttcccgcctc
     4141 gcaggtgaac ctcgcccccg aggtggggga ctacatcagg gggctcggcc tgtccgatcc
     4201 cctcattctc gccccggaca agggggccgc tcccttcgcg gccgccgttg cctcccgggg
     4261 gggctgggag acggatcacc tggaaaaaac ccggatctcc ggtaccgagg tacgggtcga
     4321 accaagaagg ggggaggcct ggaccgggga cgtggtactt gtggacgaca ttatctccac
     4381 gggtggcacc cttgcgacgg cggcccggat gctcagggcc cagggggcga ggggggtcca
     4441 tgcggtctgc gtccatggcc tcttcgcggg gagcgccctc ggcctgctcc gctcggccgg
     4501 tatcgacctg atcgcggcga gcgacaccat cgagggaccg gagagccgct actcggccgc
     4561 gggacccatc gcccgggccc tctcccgatg atcaccatcg acggttccca gctcgagggg
     4621 ggagggcaga tcctccgggg ggccgtggcc ctctcggccc tttcgcggac gccggtcgag
     4681 gtgatgcgga tccgggcccg gagggggaag cccgggctcg ccccccagca catcgcggct
     4741 gtccgtgccg tggccgcggc ctgcgatgcc acctgcgacg ggctccgggt gggaagcgac
     4801 cggatcgtgt tccatcccgg ccttcccgtg gcccgagaag tccgggccga cgtgggtacc
     4861 gcggggtccg tcgccctcgt cctccaggca tggctccccg tcgccctcgt ggcagggggc
     4921 tccatcaccg tgaccggagg gaccgaggtg gaccggagcc cgaccatcga ttacctggag
     4981 caggtcctct tccgggtcct cgcccggcac ggggcaaaga tcaccaccga gatcctgaag
     5041 aggggctatt accccgaggg cgggggagag gtccgggtct cggtcgagcc ctgccggctg
     5101 gcccccctcg ccctatccga cgaaaagccg gggtcgtgcg gcatcatctc ctgctcggcg
     5161 aaccttccgg accatgtcgc agagaggcag gccggtgcgg cacggagact tctcgaggga
     5221 agactgaaga tcccctgcga ggtcaggctt gaccggcgga ggggagcctc gaccggcacc
     5281 tcctgcaccg tctgggctgg cgcaaagggc gggatcagcc tcgggaagag gggggttccc
     5341 gcggaccgtg tcggggagat ggcggcccgg gccctcctcg acgaggtcgg ccgcggggga
     5401 caggtggaca cccacctcgc cgaccagctc ctcgtcttcc ttgccctgtc aggcggacgg
     5461 tacaccgccc ccgcctgctc ccttcatgcc gccaccatgt gctggctcct ggagcggttc
     5521 gggtaccggg tccattcccg gacgaacggc gtggtggagt tctctgcatg agatacgccg
     5581 tcgacgcctc cttcttcttc tccgagctcg tcctggacgg ggagatctcc accccgccct
     5641 ccatcgtgga ggagctcgca gacgcccgtt cccggtgccg tctggaggcc ctcctcgccg
     5701 cggggctctc ggtcactcct ccctctcccc cgagcctgaa gcgggtcgcc ggtgccgccg
     5761 cggagaccgg ggacaccggc cggctctccc ccgcggacac cgacctcctc gctctctccc
     5821 ttgacctggg cgcaaccgtc gtcaccgacg attatgccgt ccagaacgtg gccatccggc
     5881 tcgggctcgg ggtaaggagc atcctccaga aaagggcccg ctccaggcaa tggagattcc
     5941 ggtgcccggg ctgcaaccgc aggtatcccg cggccggcac ctgcccggcc tgtggcacgg
     6001 tcctgaaacg aagccttaag taggatgcct cgtgaacctt cctctcatgt cctctctcga
     6061 cgacctcatc cggagggccc ggatgctcca tgccgagggg cacagccaga gccagatctc
     6121 cgacgagctg aacctctcca tggagacggt cacgtacctc ctcatgaagg agaaggaggg
     6181 aggggtcccg agggacgtgc tcatcgactg gacgccggtg agcgggaggg cggacctcct
     6241 cgacgacctg gcggccctca tgatcggccg gtcgcggtct cccggggaag gccccggggg
     6301 ggagggggga gagccgaccg cggtggtggg gatcgccctg tccggcgttc ccctcgcgac
     6361 cctcatcgcc ctccaggagt gcctgaagct cgcggtctac ttcccttccc ggcactccac
     6421 cgccgacccc ccggtggggt ccctctcagg gaatttcgct ccggtgaacg gggagggctg
     6481 catcctcgtg gacgatgtca ttacctcggg aaagaccctc ggggaggcgg tggactacct
     6541 gaggggccac ggggcaaacc ccctcgccat ctgggtcatt ttcgacaaga gaggcctgcg
     6601 ggagatcgac ggggtgcccg tcctggccct cttccgggtc tcgcggctcg actgaatcct
     6661 accgattttt ttatatagaa gtttaaatcc atgaatattc acgcattagt gaggttactt
     6721 tatgctcgca ggacaaccag tcatcatcct cagggataac gtcgagcgga cccggggcca
     6781 cgatgcccag cgctcgaaca tcgcagcggc aaaggccatc gctgcggcgg tccgcaccac
     6841 cctcggaccg aggggaatgg acaagatgat catctcctcc accggggaca tcgtcatcac
     6901 gaatgacggg gcgaccatcc tccacgaact ttccatccag catcccggcg gaaagatggt
     6961 ggtcgaggtg gccgagaccc aggacgacga ggtgggcgac gggaccacca ccgcctgcat
     7021 cctcgtgggt gccctcatgg aggaggccga gtggatgctc gagcaggaga tccacccgac
     7081 ggtcatcgcc caggggtacc ggatggccat ggaaaaggcc ctggagatcg tgaagaatct
     7141 cgccatcacg gtgaagcccg acgacgtgaa gaccctgaag atcatcgccg acaccgcgat
     7201 gaccgggaag tccatcgagc aggtgaagga gaagctcgac gggatcgtcg tggatgccgt
     7261 ccgggccgtg gtccagacgg tagacgggaa gctcgtggtg gacgaggacg acgtgaagat
     7321 caagaagcac gtcggggaaa ccatcgacga cgccgagttc atccggggga tggtcgtcga
     7381 gaaaaagcgg gtctccgagg agatgccgaa gaggatcgag aaggcgagga tcgccctcct
     7441 cgcgaccccc ctcgagatca cgaagacaca ggtgaagtcc aagatcaaga tcacctctcc
     7501 cgaccagctc atggccttcg acacccagga gcggggcgcc ctccacgaga tggccgagca
     7561 ggtcatcaag acgggcgcga acgtgatcct ctgccagaag gggatcgccg actcggtcca
     7621 gtactacctc gcgaaggagg gcatcatggc catcgaagat gtcccggaga aggacatgct
     7681 ctttgccgcc cgggccttaa acggccagat cgcgaacaag ccccatgacc tcacggcaaa
     7741 gatgctcggc catgccgagc tcgccgagga gatggagggg atcgacctga taaagatctc
     7801 cggctgccgg aacccccggg ccgtgaccat cctcgtccgg ggctccaccc aggtcctcat
     7861 cgacgagatg gagcgggcgg tctatgacgg gatccgggtg gtgatggacg ccatcgagga
     7921 ccagaagtat gtcgtcgggg gcggtgccgt agagaccgag gtgatgctca gggtacggga
     7981 ctatgccgcc tctgtcggag gcaggatcca gctcgccatc gaggcctttg ccaatgcctt
     8041 cgagagcatc ccgaggaccc ttgccgagaa ctcgggcttc aaccccatcg acaagctcgt
     8101 ggagctgaag acggcccatg ccaaggggaa gaagcgggcc gggctgaacg tctataccgg
     8161 aaagatcgtg gacatgttcg acgagaacgt attcgagccg atgcgggtga agacccaggc
     8221 catccagtct gcggccgagg cctcgaacct cctcatccgg gtggacgaca tgatggtcac
     8281 ccagaagaag gaaccggcca tgccgcccgc cggggccatg ccccccatgg gctgatcccg
     8341 aaggtacccc atcttttctc gctccttttc atcagatgga caggccccgg ccagggcgac
     8401 ccctccgata aaattatggc tgtctgaggc gaacgcttca cctctcctgc cggggtagtc
     8461 tagcccggga aggcgtcggc ctcgaaagcc gatgttgctt ggcaacacgg gagttcaaat
     8521 ctcccccccg gcgtcccaac ctgtacgaaa atatctgtcg gcctcgaaag ctgatgttcc
     8581 ctggcaacac gggagttcaa atctcccccc cccggcgttc caacctgtac caaaatatcc
     8641 gtcggcctcg aaagccgatg cttcctgcca acactctttt atgaacaatc cttataaggc
     8701 tccccctccc accatctctt taaccgcatg attggcctcg cgtgagtgca tgttcgagca
     8761 ggatgtggtg aagcccggat atatttttgg atgcatcctc tcgctcctcg ccctgtcatg
     8821 ggggatcccc atcctcgttg cgcccggttt cctccccggc tggtcgcttg gcctcatccc
     8881 cttcctcccc ggcatcctcg ccctcgtgtt cctctccctc gaggaccatc ctatccaggt
     8941 ccatgcccgg cccctcttcg gcccggtcaa ggtacccgcc gtgatcctgt cggtggcgta
     9001 cccgttcctc cttctcggcc tcgccgccct cgtcgccctc gggaccgggc tcgggaacct
     9061 gaacccgggg gccggtacgg ggatcgatac ggcccttgcc gccggtgtcg tggccttcct
     9121 cgtcgccatc ccggcctccc ttctgcagga gtacgggtac cgggggtacc tcctcccggc
     9181 cctcacctac tggaggggga agattgccgc caccctcatc gtcgggatcc tctgggggct
     9241 ctccatggcc cccttctcgt acctggttct ctcgggggcc ggtgccgggg accccctcac
     9301 ccttgccttc ctcgggttcc tcctcacggt ctccgtggcc ttcgccttct cctcctgcta
     9361 ttacctctcc gagagcatcc tcccggtcac cctgatgaac gtcctcctca ccatcctcgt
     9421 ccccgccttc ttctccgcag gctggaatcc aacgggggct gcctcgggcg gcctcatcgg
     9481 ggtgacctgg ccctctcccg tccccctcct cctcctcgtc gccctcgcct tcgtcccggt
     9541 catcgcctgg ctcttcacgg tcatggacgg cgagatgttc ggcgatgaac tctgaaaggg
     9601 cgtttaccga atctgctccc cgggtttcgg actcctcccc gaggtaagac ggccggaccc
     9661 gcccggtggt ttttatacga agggcggttc atgtctctat ggagtctctt ctttgagcga
     9721 acccgaatcc atcatcgagg tacaggggct ctcccactcc ttcggggagg tggttgccgt
     9781 ggactccatc tccttcaccg tccggaaggg ggagatcttc tccttcctcg ggccgaacgg
     9841 ggcggggaag agcacgacca tcaacgtgct caccacgctc ctcccgatcc agaaggggca
     9901 ggtgcgcatc gccgggcatg acgtggccgc cgaacccggg gaggtccgga ggtccatcgg
     9961 gatcgtcttc caggaggaga ccctggaccg ggacctgacc gtgcgggaga tcctcgagtt
    10021 ccacgggagg ctctactcga tgccccggga cgaacgccgg gcgaggatcg acgagatgat
    10081 ccggctcgtc gagcttgaga agaagcggga cgttctcgtg aagcacctct cgggggggat
    10141 gaagcggagg ctcgagatcg cccgggggct catgacgagg ccgggggtgc tctacctgga
    10201 cgagcccacc ctcggcctcg acccccagag ccggctccgg gtctgggact acatcaggca
    10261 ggtgaaccgg gaggggacca ccatcttcct cacgacccat tacatggacg aggccgacag
    10321 cctctcggat cggatcagta ttatcgatca cgggaagatc atcgcctcgg gaacgagcga
    10381 ggagctgaag aacgccctcg gggaggacat gatctacctc gagaccgggg acgatgcgaa
    10441 gaccacgggg atcctccgga acctccccga ggtgaaggac gtgaaaccct cccttcgggg
    10501 gctttcggtc accctgaaca acgaggggac aaagaccctg ccggtcatcc tggaccggct
    10561 gaaggagggc gggatcgaga tccggtcggc gaacctgaag aagccgaccc tggacgatgt
    10621 cttcgtccac ttcaccgggc gggagatccg gggatgaggg ggtgaaagag tggcgactga
    10681 gttcgtaacg gtgtactggc gggacatgct ccggttcttc cggttccggg cgctcctctt
    10741 ctcatccctc gtccagcccg ccctctggat ggccttcttc ggggtcgcct tcacggggac
    10801 cctctcccag ctgggggccc ccaccaccgc ccccccgggg gccctcgccg tcccgtacct
    10861 caccttcatg gcagcgggga tcatggccct cacgaccctc ttcacgagcc tcttcggcgg
    10921 gatcagcctc ctcttcgaca agctctgggg gctcatgcgg gagatcctgg caagccccat
    10981 gccccggccc cacattctca tcggcatcag cctctcgggg atgaccaagt ccttcatcca
    11041 ggtgctcatc atcatgctct tcggccttgt catcggggtc cagttcttcg tcggggcctc
    11101 gcccctcatg gtcctcgtct ccgtcctcgg gatcctcctc ttcgtcgggg tcttctccct
    11161 cgggttcctc ttcatgtccg ccaccatctc gatgcggatg gagagcccgg agagccttca
    11221 ggcggtgatc accctcctct ccctcccgct cttctttgtc tcgaacgccc tctacccggc
    11281 cagtgccttc ccctccttcc tccaggcagc gacggcagta aaccccctca cctacgtggt
    11341 cacgggcatc cggtactttg ccatcggccc tgacttctat gccctcggga gccactatgt
    11401 ctataccctg atggacatgg gattcgcctt cctcgccctc gtcgtctttg ccctcgtcat
    11461 gttcggcatc gcctggtgga ccttcgagaa ggccgtggtg acctgaggtg ggacgggtgg
    11521 gggtgaagcc cgggaaacaa cccggcggag gcgggtctgc ccccgggatc accatcctcc
    11581 gcgaggactt cctgacgacg gtatccatcc ctccgggctc cgtcgacctg gtcgtcacct
    11641 cgccgcccta caacgtgagc atcccctacg ccacggtccg cgacgatatc tcctataccg
    11701 agtacctcga gttcacccgg cggtggctcg cgaaggcctt caccctgatg aagcccgatg
    11761 gcaggatgtg cctgaacatc cccctcgaca agaacctggg cggccagcag tcggtgtacg
    11821 cggacatcct cgggctcgcg aaggcctgcg gctggcggta ccacgcgaca atcatctgga
    11881 acgaacagaa catctcccga cggacggcct gggggtcctg gctctcggcc cgagccccgt
    11941 acgtgatcgc cccggtcgag accatcgcgg tcctgtaccg cgggacctgg aagaaggccg
    12001 agcccgggaa atcggacatc acccgggacg agttcatcgg gtggacgaac ggggtctgga
    12061 ccttttccgg cgagaaacgg ggaagggcga accacccggc acccttcccc ctggagctcc
    12121 cgacccggtg catcaagctc ttcacctacg tcggggatac cgtcctcgac cccttcatgg
    12181 ggagcgggac caccctcctc gcctgccgga acctggggcg gaccgggatc ggggtcgata
    12241 tcgatgccgc ttactgcgag gtggcaaaaa agaggctctc cgggtgaggg ggaggaaggg
    12301 atcccgggac ttccctgaac atgcgtaaag ccttcaccct tcgggaaaaa gtcccgaacc
    12361 ggtggaggtc tctaggtgat ctccttaaat ttctttttaa acgccttgca gatggggcac
    12421 tggtcgggca ccacatcgag gaagatgttc ccgcagatgg g
//
LOCUS       DATGDI010000090         1678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_212210, whole genome shotgun sequence.
ACCESSION   DATGDI010000090 DATGDI010000000
VERSION     DATGDI010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212210"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1499
                     /locus_tag="VKO45_03395"
     CDS             186..1499
                     /locus_tag="VKO45_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HMA04949.1"
                     /translation="MNEGGTIKQLRKQNEQLKRENELLKAKIVELEARLARYENAHTP
                     PSLRHGGSRKKDQNEGAQGKPGQKKGHKGLTRPYAKPDKQIDVTRDRCPDCGANLGSP
                     FKIESKIIEEIPEPQSVTVTEYRIAHYRCQRCQKVVTGTNAVCPDRGKFGNNTIAQAT
                     LLKYEDRLPHRKIQNALKRLFGLNISPATIFDLTRRAADAIQSDYDSILDRVRKAQIL
                     YVDETSIDVQGKKHWIWVFTTPSEIFVAIRESRGMKVLNEVLGRFEGIIVCDGWKPYV
                     KFTKNIQRCWAHLLRESKDLALKIPEAIPIHGALKCIYERLIDALKDDPPPEIRKVLW
                     YMARALLKRLIEGKYENENIRMLMSKISNGFEYWFTFVVHPDVEPTNNRAERAIREHV
                     VQRKIIGTLRNEKGTSIHQRIMTVLATWSLLRLDSLQMLRLKLSG"
BASE COUNT          560 a          310 c          377 g          431 t
ORIGIN      
        1 gaaatcttct tcctaagcat ggcgtgatga gacaatttta catatttgat gtgaactgag
       61 actaagatta tttgttatat tggtacgtgt ttagctgtcg ttggagtaac tctaggaatt
      121 ccatcagaat tcacctagac cagaaaaatt aagtaaaata aaatcaataa tcttttttta
      181 ggagtatgaa tgagggcgga actatcaagc agcttcgcaa gcaaaatgaa caattgaagc
      241 gagagaatga gctgcttaaa gccaaaattg ttgaacttga agctcgacta gcccgatatg
      301 agaatgcaca tacgccaccg agtctgagac atggtggcag tcgcaaaaag gatcaaaatg
      361 aaggtgccca gggaaagcca ggtcagaaga aagggcataa aggcttgaca agaccttatg
      421 cgaagcctga taaacaaatt gatgttacga gagatcgatg tcctgattgc ggtgcaaatc
      481 tcggcagtcc ttttaagata gaatccaaga ttatagaaga aattccagaa cctcaatctg
      541 tcactgttac cgaatataga attgctcatt acagatgcca acgatgtcaa aaagttgtaa
      601 ctggaaccaa cgcagtttgt ccagatagag gtaaattcgg caacaatacg attgctcagg
      661 caactctgct taaatatgaa gatcgattac cccacagaaa aatccaaaat gcgctaaaga
      721 gattattcgg tctgaatata agccctgcaa caatatttga tctaactcgt cgtgcagcag
      781 atgcaataca atcggattat gattctattt tggacagggt tcgcaaagcc cagattctgt
      841 atgtagatga aacatcaatc gatgttcagg gtaagaaaca ctggatctgg gtattcacaa
      901 ccccctccga gatattcgtc gcaattaggg agagtcgggg catgaaagtt ctgaatgaag
      961 ttctggggag gttcgaggga ataattgtat gcgatggttg gaaaccgtat gttaaattca
     1021 caaagaacat acaaagatgt tgggcacatc tactaagaga atccaaggat ctcgcactga
     1081 agattcctga agcgattcca atacatggcg cactcaaatg catctatgag cgactcattg
     1141 atgctctgaa ggatgatcct ccacctgaaa ttcgaaaagt tttgtggtat atggcaagag
     1201 cattgcttaa gcggttgatt gaaggtaagt atgaaaatga gaacatcaga atgctaatga
     1261 gcaaaattag caacggcttt gagtattggt ttacttttgt cgttcatcct gatgtggagc
     1321 cgacaaataa cagagctgaa agggctattc gagaacacgt tgtccagaga aagattattg
     1381 ggactttgcg taacgaaaaa gggacttcga ttcatcaacg gataatgacc gtacttgcta
     1441 cctggtcact actaaggctt gatagtctgc aaatgctaag gttaaagctg agtggctaaa
     1501 cacataccat ttgttattat cccactcatt ttattcgcat ttggcgcgat gtattctccg
     1561 aatgactcta aaaaattaag aaaacaattc aacgagcagg atcaagcagg attataccaa
     1621 aggattggaa ggcgaacaac tcattaccca gacgctgaag aattgtaatg cccccggt
//
LOCUS       DATGDI010000091         1686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_357452, whole genome shotgun sequence.
ACCESSION   DATGDI010000091 DATGDI010000000
VERSION     DATGDI010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357452"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..869)
                     /locus_tag="VKO45_03400"
     CDS             complement(129..869)
                     /locus_tag="VKO45_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HMA04950.1"
                     /translation="MPAPLVGHKFGVGLVPPLGPGKAKGIKVLGISGSSRQVPGISKS
                     ERLLIRALDRYKGYGCETTFLRLKDLKIYDCEGNYSENPSYCTYPCQSTLKYDDDEMQ
                     TVYDAVLSTDILILATPIRWNNHSALVQKFIERMNAIENQDSWFGNRLIINKVVGLII
                     IGHVDGIQHVAGNLLNFFVWSGFHIPEAAIASWVGENDEDTTKDWDQMERNKYTQEDL
                     QNMIESTLRLSYNLKRGAGEGEEPPGTP"
     gene            complement(923..1333)
                     /locus_tag="VKO45_03405"
     CDS             complement(923..1333)
                     /locus_tag="VKO45_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04951.1"
                     /translation="MDGKKILIAGIAGGILLFIAQFVFSILGNLVSPYNIFAIGGMRQ
                     VNDPLMALFYAYPFVLSFMSAIVFDRVKPALAGSCCSGADFGLILIALVTVPGLFVIF
                     SSMTYPLGFYIGNILFGIIGFPVVGLLYARIWKI"
     gene            complement(1438..>1686)
                     /locus_tag="VKO45_03410"
     CDS             complement(1438..>1686)
                     /locus_tag="VKO45_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMA04952.1"
                     /translation="NAKIESCATGSEGLSLLKSDVAITDANKVTGSPTLIINGQRYSG
                     ARTPDAFKQGICSHFTTSPAECSVNLSAQAAAASGSCG"
BASE COUNT          335 a          466 c          527 g          358 t
ORIGIN      
        1 ctcttcctcc tcttcgtctc cctgccgaac ccgtggtttg ccctctcggg cctgttcctg
       61 attcccgccg ggtggttcat cctggggaat gcccttgccc ggtggaacgc ggtggagcag
      121 ccgggatttt agggagtacc aggcggttcc tcgccctccc ccgcccccct tttcaggttg
      181 taggagagcc tgagagtgct ctcgatcatg ttctggagat cctcctgcgt gtacttgttc
      241 cgttccatct ggtcccagtc cttcgtcgtg tcctcgtcat tctctcctac ccaggaagcg
      301 atggccgctt cagggatgtg gaacccggac cagacgaaga agttcagcag gttcccggcc
      361 acatgctgga tcccgtcgac atgcccgatg atgatgaggc cgaccacctt gtttatgatc
      421 agccggttcc cgaaccagga atcctggttc tcgatggcat tcatccgttc gatgaacttc
      481 tggacgaggg ccgagtggtt gttccaccgg atgggggtcg cgaggatcag gatgtccgta
      541 gagagcacag cgtcgtagac ggtctgcatc tcgtcgtcat cgtacttgag cgtggactgg
      601 cagggatagg tgcagtacga ggggttctct gagtagttcc cctcgcagtc gtagatcttc
      661 aaatccttca gccggaggaa ggtggtctcg cacccatagc ccttgtaccg gtcaagggcc
      721 cggatgagga ggcgctcgga cttggagata ccgggcacct gccggctcga ccccgaaatc
      781 ccgaggacct tgatgccctt tgccttcccg ggtcccaggg gtggaacgag ccccacaccg
      841 aacttgtgcc ccacgagtgg tgctggcatg ggagatcatt ccatgccggg ggataagaaa
      901 atgtccatta cctgttattc ctctagatct tccagatcct cgcgtagagc aatcccacga
      961 cggggaaccc gatgatcccg aagaggatat tcccgatata gaacccgagg ggataggtca
     1021 tggagctgaa gatgacgaac agtccgggca ccgtaacgag ggcgatgagg atgagcccga
     1081 agtcggcgcc gctgcagcag gatcccgcga gggccggctt cacccggtca aagacgatgg
     1141 ctgacatgaa ggagaggaca aagggatagg catagaagag ggccatgagg ggatcattca
     1201 cctgcctcat tccaccgatg gcgaagatgt tgtacggaga aaccaggttt ccgaggatac
     1261 tgaagacgaa ctgggcgatg aagaggagga ttccacccgc gatccccgcg atgagtatct
     1321 tcttaccgtc catgggaggg ggtgcttccc ggggagagaa aaagctggtg gaaaggttct
     1381 gaaggtgatt tgagcttgcg aaaacggatg aatatttcct gaaaaagggc ggggggatta
     1441 accgcagctc cccgatgccg cggcagcctg agcagagagg ttgacactgc actcggcagg
     1501 cgaggtggtg aagtggctgc agatgccctg cttgaaggcg tccggggtcc gggcgccgct
     1561 gtatctctgt ccgttgataa tgagggtcgg cgatccggtg accttgttgg catcggtgat
     1621 agccacgtcg ctcttcagga ggctgagccc ctcgctcccc gttgcacagg actcgatctt
     1681 cgcgtt
//
LOCUS       DATGDI010000092         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_212277, whole genome shotgun sequence.
ACCESSION   DATGDI010000092 DATGDI010000000
VERSION     DATGDI010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212277"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VKO45_03415"
     CDS             <1..1196
                     /locus_tag="VKO45_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HMA04953.1"
                     /translation="TCRLFGTVKALSTVKRSAILVHGPKGCVYHINYILGMRGDRPGA
                     IYTTSLGEHDVIFGAEGKLAEAIGELDRRLSPDLIFVLSCCASSIIGEDVGSACRAAR
                     SRARVIGFECGGFEGEHHTAYGETLSRLAEELALPAEERRPASVNLIGMLRAGPDLRE
                     LKRVLSLAGIEVLGVLPADATLRDLEGLGRAALNVVVCEPAGRVAAETLERRFGTPFI
                     VEEFPIGARAALSFLGHVTSALGIPLDPGIFAGEPEPIHPAGEGVGLERELRIAVVGG
                     PTRAVSMVRFLGEQGLAPVLVAVDFDAGAGERLREFAMPGLEVLVEPSQEEILERLRA
                     RGVNLLIGGMFERPVAAMLGIPFLDMMHGSQRTACFAGESELSRMIAGIRRGRSGVDG
                     GPGSS"
     gene            1350..2480
                     /locus_tag="VKO45_03420"
     CDS             1350..2480
                     /locus_tag="VKO45_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HMA04954.1"
                     /translation="MATREPFDRSARSGVGNMESGNRRSTAGVDRAREAAIRGILPPH
                     MLKHLAESRDPDARSLGIRMLMRIEKIRGMRRALGQFYVAPPGGARCIRVYDVKHGSD
                     SDLPGDPVANPARSRDPEAKEAYQGAVATYNFYKDIYGRNSIDDRGMCIDSSVHFETD
                     YDNAGWDGKQMIYGDGDGVYFNRFTIAIDVIGHELTHGVTEYEAALEYHDQPGALNES
                     MSDVFGTLVKQWKAKETVDAADWLIGEGLFTKKVKGKALRSMSAPGTAYDDPVLGKDP
                     QPADMKHYVKTTEDEGGVHINSGIPNHAFYLTAMELGGYAWEKAGRIWYVTLRDALRP
                     TNSFTTAAKRTYQVAGVLFGTGSEEQQAVRTGWTGVGVKVPK"
     gene            2493..2789
                     /locus_tag="VKO45_03425"
     CDS             2493..2789
                     /locus_tag="VKO45_03425"
                     /inference="COORDINATES: protein motif:HMM:NF042532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protealysin inhibitor emfourin"
                     /protein_id="HMA04955.1"
                     /translation="MIIHLVRSGGFGGIRREVRIETLSLTREEREPLERMVGETGFFT
                     LPEKFPRPARGADFFIYTITVDDGNRRHTVEVAQPSVPEGLRPLVRELSRRLKS"
BASE COUNT          495 a          904 c         1023 g          439 t
ORIGIN      
        1 gcacctgccg gctcttcggc acggtgaagg ccctctcgac cgtgaagagg agtgccatcc
       61 tcgtgcacgg gccgaaggga tgcgtgtacc acatcaacta catccttggg atgagagggg
      121 accgcccggg cgcgatctac accacctcgc tcggtgagca tgacgtcatc ttcggggccg
      181 agggaaagct cgcggaggcc atcggggagc tcgaccggag gctctctccc gacctcatct
      241 tcgtcctctc ctgctgcgcc tcctccatca tcggggagga tgtggggagc gcctgccggg
      301 ccgcccggtc ccgggcccgg gtgatcgggt ttgagtgcgg cgggttcgag ggggaacacc
      361 acaccgccta cggggagacg ctctcccggc tcgcggagga gctcgccctg cctgcggagg
      421 agaggcgccc cgcgtcggtg aacctcatcg gcatgctcag ggcgggcccc gacctccggg
      481 agctgaaacg cgtgctctcc ctcgcgggga tcgaggtcct gggggtgctt cctgccgatg
      541 cgaccctccg ggacctggag ggcctcggga gggcggccct gaatgtcgtg gtctgcgagc
      601 cggccgggag agtcgccgcg gagaccctgg agaggaggtt cgggaccccc ttcatcgtcg
      661 aggagtttcc catcggggca cgggccgccc tgtccttcct cggccacgtg acctcggccc
      721 tcgggatccc cctcgatccg gggatcttcg ccggcgaacc ggagcccatc caccctgcag
      781 gggagggcgt ggggctggag agggagctcc gtatcgccgt ggtaggcggg ccgacccgtg
      841 cggtctccat ggtccggttt ctcggggaac agggcctcgc ccccgtcctc gtggcggtgg
      901 acttcgatgc cggggccggg gagaggctcc gggagttcgc gatgcccggg ctcgaggtcc
      961 tggtcgagcc ctcgcaggag gagatcctgg agaggctccg ggcccggggg gtgaacctcc
     1021 tcatcggggg aatgtttgag aggcccgtcg cggcgatgct cgggataccc ttccttgaca
     1081 tgatgcacgg gagccagcgg accgcgtgct ttgccggcga gagcgagctc tcccggatga
     1141 tcgccgggat cagaagaggt cgctccggag ttgacggcgg ccctggaagc tcctgacccg
     1201 gcttcaccgg ggtcccggcc ctccctcgcg gctctcatcg acggccccat tcacgcgggg
     1261 gacgggccat agttttatat aatgcggaca agagagcaac gtccgccggc agggggtccg
     1321 ggggtagttc ccttgaggag tccctgcaca tggccacccg ggaaccgttc gaccggtcgg
     1381 cccggagcgg agtggggaac atggagtcgg gtaacaggag atccaccgcc ggggtggaca
     1441 gggcgcggga ggcggccatc cgagggatcc tcccgccgca catgctgaag catctcgccg
     1501 aaagccggga tccggatgcc cgcagcctcg ggatccggat gctcatgcgg atcgagaaga
     1561 tccgggggat gcggcgggcc ctcggccagt tctacgtggc accccccgga ggggcccggt
     1621 gcatccgggt ttacgacgtg aaacacggga gcgacagcga tctccccgga gatcccgtcg
     1681 cgaatcccgc gaggagcagg gaccccgagg cgaaggaggc ataccaggga gcggtggcga
     1741 cgtacaactt ctacaaggac atctacggcc ggaattccat cgacgacagg gggatgtgca
     1801 tcgattcatc ggtccacttc gagaccgact atgacaacgc cgggtgggac gggaagcaga
     1861 tgatctatgg ggacggggac ggggtctact tcaaccggtt cacgatagcc atcgatgtca
     1921 tcggccacga gctgacccac ggggtcacgg agtacgaggc cgccctcgag taccatgacc
     1981 agccgggggc cctgaacgag tccatgagcg atgtcttcgg caccctcgtc aagcagtgga
     2041 aggcaaagga gacggttgat gcggccgact ggctcatcgg cgagggcctc ttcacgaaga
     2101 aggtgaaggg gaaggccctc cggtccatga gcgcgccggg aaccgcgtac gacgatcccg
     2161 tcctcgggaa ggacccgcag ccggcggaca tgaagcacta cgtgaagacc accgaggacg
     2221 agggaggggt ccacatcaac tccgggatcc cgaaccacgc cttctacctg acggccatgg
     2281 agctcggggg atacgcctgg gagaaggcgg ggaggatctg gtacgtcacc ctcagggatg
     2341 cgctcaggcc gacgaactcc tttaccaccg cggcgaagag aacctaccag gtcgccgggg
     2401 tgctcttcgg cacggggagc gaggagcagc aggcggtacg gaccggctgg accggcgtcg
     2461 gggtgaaggt acccaagtag acgggccttg tcatgatcat ccatcttgtc aggagcggcg
     2521 gcttcggggg gatccggagg gaggtccgga tcgagaccct gtccctcacc cgggaggagc
     2581 gggaacctct cgaaaggatg gtcggggaga ccgggttctt cacccttccg gaaaaattcc
     2641 cccggccggc ccggggcgcg gacttcttca tctacaccat cacggtggac gacgggaacc
     2701 ggagacatac cgtcgaggtt gcccagccct cggtgcccga gggcctccgc cccctcgtcc
     2761 gggagctctc gaggcgcctg aagagctgag cgggagccgg tcgcgattcc cgtcccgcaa
     2821 gagttatttc ctccccgtcc gacatcaggc gcgatgacag a
//
LOCUS       DATGDI010000093         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_67092, whole genome shotgun sequence.
ACCESSION   DATGDI010000093 DATGDI010000000
VERSION     DATGDI010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67092"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..985
                     /locus_tag="VKO45_03430"
     CDS             254..985
                     /locus_tag="VKO45_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA04956.1"
                     /translation="MLEITDLHVEVGEKEILHDINLRIGNGETHVLMGPNGSGKTSLL
                     MAIMGFSGYMVTQGKIMYNGKEITHLPVNERAKAGVGLMFQRPPTVGGLKLGKLLTAT
                     SGESQEAIFDYAKTVHMLTFLGRDVNKGFSGGEIKRSEILQLMVQHPDFVMLDEPESG
                     VDLENISLIGAAIGKLLEKDLHIVHRKKSGLIITHTGFILDYLDADVGLMMCDGVIKC
                     RGNPREMLKEIKERGYRECMECQKV"
     gene            970..2085
                     /locus_tag="VKO45_03435"
     CDS             970..2085
                     /locus_tag="VKO45_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HMA04957.1"
                     /translation="MPEGLNIPSSISAEDRERLRQSGIELSMINRCGSYLQANQDVMH
                     ATCSAEGIEVLSIADALKRYDWLQEYSWNVMPKDRDQYTKAVAAQENPRGYVIIGRKG
                     TKNILPVQACLYLGREQIQYVHNIIIAEEGAELHIVSGCASGRHVGKGGAHYGISEFY
                     VKKNAKISFTMIHTWNEEIEVYPRSASIVEENGTFLSNYICMQPVKKVQLYPTANLNG
                     ENSVARFSSIVIASPTSYIDVGSRAILGAKGSSAELITRAITRGGTIISRGDIQGKTN
                     GTRGHLECKGLILKDGVIHAIPEIQGTVVGTELSHEAAVGKIAQDEIEYLMARGLSEE
                     EATATIIRGFLDVRITGLPEVLQRQIDAAIDKAESGF"
     gene            2088..>2444
                     /locus_tag="VKO45_03440"
     CDS             2088..>2444
                     /locus_tag="VKO45_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HMA04958.1"
                     /translation="MPADPFTEERRRMVEEQIRARGIRNERVLAAMEKVPRHLFVPGD
                     LRRASYEDRPLPIGEGQTISQPYIVALMTDLLDPRAGDRVLEIGAGSGYQAAILSELA
                     SEVISVERIPSVAAQAR"
BASE COUNT          508 a          748 c          781 g          407 t
ORIGIN      
        1 catcgcccgg gccggatccc tcaggatcgg gcgcggctgg ttctttttca cgataaccgg
       61 gtaccgggcc gccctcgcgg cgatgaaccc gaaggttgaa tcgatggttg ctccgtgcat
      121 gggaaagggg tgaggaaggg acgataaacc ccttcggggt gcgcggggcg aaagtatcag
      181 gattcccggc ccccgtcgga tggccagagg ggagccttta aacatctggt gagagaatta
      241 catctgcgtt catatgcttg agatcacgga cctgcatgtc gaggtggggg agaaggagat
      301 cctccatgac atcaatctca ggatcgggaa cggggagacc catgtcctga tggggccgaa
      361 cggctcgggg aagacaagcc ttctcatggc catcatgggc ttctcgggct acatggtcac
      421 ccaggggaag atcatgtaca acgggaagga gatcacccac ctgccggtga acgagcgggc
      481 gaaggcgggg gtaggcctca tgttccagcg ccctcccacg gtcggggggc taaagctcgg
      541 gaagctcctt accgccacat ccggagaatc tcaggaggcg atctttgact atgcaaagac
      601 ggtgcacatg ctcaccttcc tgggcaggga tgtgaacaag gggttctcgg gaggggagat
      661 caagcggagc gagatccttc agctcatggt ccagcacccc gacttcgtga tgctcgacga
      721 gccggagagc ggggtcgacc tggagaacat ctccctcatc ggggcggcca tcgggaagct
      781 cctggagaag gacctccata tcgtgcacag gaagaagagc gggctcatca tcacccacac
      841 cggcttcatc cttgactacc tggatgccga cgtcggcctc atgatgtgcg acggggtcat
      901 caagtgccgg gggaacccga gggagatgct caaggagatc aaggaacggg gctaccggga
      961 gtgcatggaa tgccagaagg tctgaacatc ccttcctcca tctctgccga ggaccgcgag
     1021 cgcctccgcc agtccggtat cgagctctcg atgatcaacc ggtgcgggtc ctacctgcag
     1081 gcgaaccagg atgtcatgca tgccacctgc tctgccgagg ggattgaggt cctctccatc
     1141 gccgatgctc tcaagcgata tgactggctg caggagtact cctggaatgt catgcccaag
     1201 gacagggacc agtacacgaa ggctgtcgcc gcccaggaga acccgagggg atatgttatc
     1261 atcgggcgga agggaacaaa gaacatcctc cccgtgcagg cgtgcctgta cctggggcgc
     1321 gagcagatcc agtacgtcca caacatcatc atcgccgaag agggtgcgga gctccacatc
     1381 gtctccggtt gcgcgagcgg caggcatgtc gggaaaggag gtgcccacta cgggatctcg
     1441 gagttctacg tgaagaaaaa cgcaaagatc tccttcacca tgatccatac ctggaacgag
     1501 gagatcgagg tgtacccccg ctccgcctcc atcgtcgagg agaacggcac cttcctctcc
     1561 aattacatct gtatgcagcc ggtgaagaag gtccagctgt atccgactgc gaacctgaac
     1621 ggcgagaact cggtcgcccg cttctcgagc atcgtcatcg cctcccctac ctcgtacatc
     1681 gacgtaggtt cccgcgccat cctgggggca aagggatcga gcgccgagct catcacccgt
     1741 gccatcaccc gcggcgggac catcatctcc cggggcgaca tccaggggaa gaccaacggg
     1801 acccgggggc atctggagtg caagggtctc atcctcaagg acggcgtcat ccatgccatc
     1861 cccgagatcc agggaaccgt tgtcgggaca gagctctccc acgaggcagc agtcggaaag
     1921 atcgcgcagg acgagatcga gtatctcatg gcgcggggac tctccgagga ggaggccacg
     1981 gcgaccatca tccggggatt cctcgacgtg aggatcaccg gcctccccga ggtcctccag
     2041 cggcagatcg acgcggccat cgacaaggcg gagtcaggat tctagccatg ccggcggacc
     2101 ccttcaccga ggaacgccgc aggatggtgg aggagcagat ccgggcccgg gggatccgga
     2161 acgagcgggt gcttgcggcg atggagaagg tcccccggca cctcttcgtc cccggggacc
     2221 tgcgccgggc ctcctacgag gaccggcccc tccctatcgg cgaggggcag accatctccc
     2281 agccgtacat cgtcgccctg atgaccgacc tccttgaccc ccgcgccggg gaccgggtcc
     2341 tggagatcgg tgccggcagc gggtaccagg cggcgatcct ctcggagctc gcctccgagg
     2401 ttatctccgt cgaacgcatc ccctccgtgg ccgcccaggc gagg
//
LOCUS       DATGDI010000094        20054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_299606, whole genome shotgun sequence.
ACCESSION   DATGDI010000094 DATGDI010000000
VERSION     DATGDI010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 20054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20054
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299606"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VKO45_03445"
     CDS             <1..685
                     /locus_tag="VKO45_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HMA04959.1"
                     /translation="PAVLETYREQLRVGRDEYAMKLDTIVRKTPQGIYLVEAGGPEPG
                     VGCAGRGVLTALQILKDLKAFETYGIDVAIYDVLGDVVCGGFAMPIREGFAREIYLIC
                     SGGFMSIYAANNIARAVQRLARRGDTGLSGIICNSNGDEAFEHAFVPEFARRLGTTFV
                     RFVPRSPVIQACELEGKAVVEHSPESREAGIFRDLARDIMGNDSRVIPTPVEDLAELE
                     QLYRKYRKG"
     gene            complement(749..1237)
                     /locus_tag="VKO45_03450"
     CDS             complement(749..1237)
                     /locus_tag="VKO45_03450"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HMA04960.1"
                     /translation="MEEVIPNLFIGDLGDNELVRARPDWAVIHAEKELHHEYVERHRL
                     VFQDVVLTPGGNELYLAFEDAMKVDQVNTRCIGPALEFVHRQLADGKKVLIHCIAGVS
                     RSPTIAMLYLLKYTDVLPRTNIFDAIFSFSELYPLYNPNDGLFAYAAKFLEQIKGTPS
                     PR"
     gene            1489..2061
                     /locus_tag="VKO45_03455"
     CDS             1489..2061
                     /locus_tag="VKO45_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HMA04961.1"
                     /translation="MDLKGSKTEKNLLAAFAGESQARTRYSFFASAAKKEGYEQISGI
                     FQETSDNEREHAQLFFNHLKGGIAEITASYPAGITGTTAANLKAAAEGEKFEWGTLYP
                     GYGDVAEKEGFADVANTFRQVAKVEAYHERRYRKLLEAVEKGTVFSRKAPIKWKCRNC
                     GYVHEAAEVPVKCPVCAHARAYFEVWVENY"
     gene            complement(2109..2759)
                     /locus_tag="VKO45_03460"
     CDS             complement(2109..2759)
                     /locus_tag="VKO45_03460"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HMA04962.1"
                     /translation="MLQSVLHLIPDTAPSLLGPTFHSGIWFYLLVFLVILIAAVFVVT
                     PVPDQSILFLAGAMAVNNQVSLAWVLVASIAGAYVGYDLNYWSGRLFSLVVCRRACPY
                     ILSAKNILRARALLDRFGPLSVVISRFIPAVNLPPFFAGLESMDYGRYMVANLAGAVL
                     WCGVTVILGYFVGSLGIIQDYVNLLLDLVILATIAAIGYAAARFVWERARNRENTG"
     gene            2861..3643
                     /locus_tag="VKO45_03465"
     CDS             2861..3643
                     /locus_tag="VKO45_03465"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HMA04963.1"
                     /translation="MTIQVIPVQGLPLIGKGDDIPALVCERIAFQDGDILVLASTIYS
                     KARGFTRRLQDITPSERAERIARRDKEDPRFVQSVLDEAREVLLEHPFILSEMAFGHI
                     GVRSGVDRSNIEDGMVILLPPDPMGGATEIRERIRSLAGKEVGVIITDTCGRAFRRGQ
                     TGNAIGWAGFPAIRDYRNDTDLFGHVLLITEEAVVDEIAGFANFVMGESHQGIPAAVF
                     RGCGRWTGHDDLYFKPAEDVIRKAICTYVGGPPGGGPVPGKR"
     gene            complement(3648..5006)
                     /gene="ftsA"
                     /locus_tag="VKO45_03470"
     CDS             complement(3648..5006)
                     /gene="ftsA"
                     /locus_tag="VKO45_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F390 synthetase"
                     /protein_id="HMA04964.1"
                     /translation="MPSSGYYSGTIETADRETLNGIIDERVRYTVRYAYENSPFYRSW
                     FDGHGIDPSGVKTHEDLLGLPVISGNTIRANQPPETKDFLFRSVGWDTVFTVHETSGT
                     SGTPKAFFLTWQDWERYAEKYARIFISQGFGRGDRVVICFPYGRNVGANTMTLAARGI
                     GMTVIPQGTCRSPGRILESYHPTGMVGSVFRFLALARELSSAGIRPSESGVARLVAGG
                     ESFAPESRAYVEEIWGCPVHNTYGSTEGTMCGECEQHLGLHVPEDLVHLDIYDPGLSS
                     FVPDGECGRIILSTLIGKGEQCGTLLLNYDTEDSATVISRERCPCGRTHMRIMNPTRD
                     AETFRAGGAFFNRIDVERGVFQRENMEYLSGEYESFLISRGREGGTVLQVSVEVLDPA
                     RIDREGIRDRFIRGFIGKNPYLRRAHDEGSLTFDLTFAGPGSLEIFRLSGRPRRLVDR
                     RR"
     gene            complement(5057..5335)
                     /locus_tag="VKO45_03475"
     CDS             complement(5057..5335)
                     /locus_tag="VKO45_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04965.1"
                     /translation="MHGGPETRRVEPPPFGDVLLEVLKGIRSEILLYGIAVLIIVVGS
                     AVFGIEVLRELEYPLLIFATFVLIIYFLANLVKAWRKSRPEEGKDRRP"
     gene            complement(5446..6129)
                     /locus_tag="VKO45_03480"
     CDS             complement(5446..6129)
                     /locus_tag="VKO45_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMA04966.1"
                     /translation="MPVAEGSFVRLSYTGKIGEKVFDTTDEAVAKEAGIHNPEAVYGP
                     VLLRVGKRHVVIGLDDALVGKEAGDEGTVEVPPEKAFGAHDEARVESVSLARFKEKPS
                     LGQSVEIENREGTVTNIIGRRVIVDFNHPFAGKSVSYRYRIEEVVESQPEQIRGLIQL
                     YTQRSMEVTVGNGVVTIHLPPAIMYDRRWLLWRSRVVQEIFEFYPEIGEIVLLESFKR
                     QEKSGEAPE"
     gene            6315..6842
                     /gene="cyaB"
                     /locus_tag="VKO45_03485"
     CDS             6315..6842
                     /gene="cyaB"
                     /locus_tag="VKO45_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HMA04967.1"
                     /translation="MEYEVKIRVEGLASIRSRLTALRIRPEGMTTERDLYLNSPTRDF
                     GRTDEALRIRATRDGTTLTYKGPKIGLGEIKAREELIVPVAGPGDTLEKILVRLGFTP
                     TALVEKDRETYRVEGTLVALDEVKGLGSFVEIEAPPGLGEAEAVALIRRVREILGITG
                     ENTTLSYLEMILATR"
     gene            complement(6832..7473)
                     /locus_tag="VKO45_03490"
     CDS             complement(6832..7473)
                     /locus_tag="VKO45_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMA04968.1"
                     /translation="MTRVLLLADLHGRYGKLDSFLALDPDLVFICGDITQFGPCEDAL
                     GLLERIEVPCLAIPGNCDPPEILDSLEESEAVSIHGRSITLGKITVTGIGGSNRTPFG
                     TPFELTEEEIGDLLEGAMERMEKNVHNILVTHAPPQGTLDRVGENRVGSPALRRHMKR
                     FDLVCCAHIHEAKGLTEEDGVMVVNPGPASEGHCALVTLGDESRDIRIELLTV"
     gene            complement(7470..8588)
                     /locus_tag="VKO45_03495"
     CDS             complement(7470..8588)
                     /locus_tag="VKO45_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HMA04969.1"
                     /translation="MRVNWRLINAARNSYASLFAACSLYGFSLRPVKSPESEVTCYSL
                     NSLNEPRFRKEIRDASCTTVVGGPHATACPREVARYADYVVVGEGEYTLPRLLTRIEE
                     GREGLVPGVVTKEWGLPADHAVRLDAYPPFSVMKGYIEISRGCPFGCAFCQTPRIFGS
                     SMRHRSIDTIAEYARRYRDVRLVSPNALAYGSDGRRPRLDRVERLLATLEGNIYFGTF
                     PNEVRPEFVSDPALDLIARYCSNTRVAFGIQSGSDRVLRKIGRGHTVADGMRAVELCR
                     DHGLTPVVDLILGFPFEEPEDEEATLDLIRWVTRSGRVRLHAFIPLPGTPLAGTSPHP
                     VSRSADRLLGRLALEGKLTGSWRDPAGRLSMPHADTSP"
     gene            complement(8563..8982)
                     /locus_tag="VKO45_03500"
     CDS             complement(8563..8982)
                     /locus_tag="VKO45_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HMA04970.1"
                     /translation="MSLIIRARGLSGGRGRGPLLVSPEPISFLGGVDPETGEVIEKGH
                     PLFGRRVGGTILVFPHGKGSTVGSYVIYALARNGRAPAAMVNTEAEPIIVVGAIIAGV
                     PLIDHPEVPVSRLEDGKMAEVDGDAGELRYEGELEAH"
     gene            9020..10186
                     /locus_tag="VKO45_03505"
     CDS             9020..10186
                     /locus_tag="VKO45_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HMA04971.1"
                     /translation="MDNTITNNPPVESEVFQLKLELQEIQAQILDLRMKNETLQKELS
                     QVKRENAQLKRMPLFVAAVVEVMENGEIYLRQQGNNQEYITQASEDLHAVLKPGTKVA
                     VNNALSIVKIIGNIYDARVRVMELEESPEVTFERIGGLKKEIEEVREAVEYPLTKPEV
                     FRRLGVEPPKGILLHGPPGTGKTLMAKAVAHEAHATFIRMSGSELVHKFIGEGAQLVR
                     ELFSLARERAPSIVFIDEIDAIASHRTHDGTSGSAEVQRTLMQLLAEMDGFDNRGDVR
                     IMAATNRVDMLDLAILRPGRFDRILEIPLPDSGARREILRIHSAPMHLGKIDLDEIVR
                     LTEHTTGADLQAVCREAGMNAVRRGAEAVEQGDYIEAVKKVRSEPPAMDNRMYT"
     gene            complement(10247..10660)
                     /locus_tag="VKO45_03510"
     CDS             complement(10247..10660)
                     /locus_tag="VKO45_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04972.1"
                     /translation="MISMKQQTLQPWVNASDVVNAVNEITVLIEGNGGIPRAKLEYYG
                     ILAVISDLEFKHQFLRMLEERCPGAHPQFVRYLHKLAENGRKYSREYRSDMHHMTSHL
                     GRLYGMYGDGIFTRMKRGRRGVPGSTLDDAVINCA"
     gene            complement(11013..11225)
                     /locus_tag="VKO45_03515"
     CDS             complement(11013..11225)
                     /locus_tag="VKO45_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04973.1"
                     /translation="MFKTDPLQFLEAAVISIGSLRTWLFMWLISLTGLLWATFLLAQG
                     VWLWVSAWLIIAIVGSNLLVMLLRFR"
     gene            complement(11302..12462)
                     /locus_tag="VKO45_03520"
     CDS             complement(11302..12462)
                     /locus_tag="VKO45_03520"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HMA04974.1"
                     /translation="MPQGACRLEGVPGTYYEILGVARGASDREIKQAYRALAMRFHPD
                     ICHEEEAGDRFRRIHEAYRVLSDPGERNRYDSLGHEDYTRYAGGSPPPWGSRHTGGFQ
                     GFGDAFDSFFRGKSWGPSAEFRPRSPSDILVKVPVTLAEAIRGCEREIEVPYTTRCVS
                     CAGTGSAGGDVRQCPGCGGTGRQGWKEAGGFPDPTSPPCGVCGGKGRVPESPCGECGG
                     WGATQATRRVKVRVPAGIDTGMRILIPGLGEEGDHEVPGGDLYVEATVLPHDMFTRRG
                     NDLETMVHVSPAGAVMGSPVELETLDGRMIRVEIPSGTQHDGTVRIQGEGMRISGRAG
                     DLVVRVRIRLPEKATARERRLYGRLLRIEKEQADAKTGLVVRYLSKVRNRGK"
     gene            12631..14115
                     /gene="purH"
                     /locus_tag="VKO45_03525"
     CDS             12631..14115
                     /gene="purH"
                     /locus_tag="VKO45_03525"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HMA04975.1"
                     /translation="MKWALLSVWDKTGLAGLARDLTSRGVSILASGGTGKALGAGGIP
                     FVEISEYTGSPEMMDGRVKTLHPRIHAGLLGRRGIDDDAMAALGIHPIDLLVANLYPF
                     EKMATQGLPLAELVEFIDIGGPAMIRAAAKNHASVAVVVDPADYPVVSAAVQRGSFTG
                     EERLALAKKAFARTAAYDGAISNHLQSLEGPLPLVYTLQARNGRILRYGENPHQAGAV
                     YGETGIAGQVPLQGREMSYNNYLDTDASVALLREFDETCCVCVKHNNPCGVATGVDPK
                     EAYMAAREVDPDSSYGGIVALNREVDAGTAEEICRTFIELVIAPSFTGDALGVFRAKE
                     KMRVLTLPDPAGGLALRSIDGGLLIQSTPPYTEHWQVVTERDPTASELLALRLAWRVC
                     KHARSNAIVFADPVRTLGIGAGQTSRVDAARIAVMKARFPLKGSAVASDAFLPFPDTL
                     EEAARAGATALVQPGGSIRDRESIEAANRLDMAMIFTGIRHFRH"
     gene            14248..14913
                     /locus_tag="VKO45_03530"
     CDS             14248..14913
                     /locus_tag="VKO45_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HMA04976.1"
                     /translation="MDIGKILNDSLGYAKDAVWEKWVRWLLLIISSIIFPLILGYELE
                     VYRGKKPAPEPTNWGGLFIDGIKLFIIELIYLIPTFIVLFLTVGAAALAVTSPAVAMA
                     MWGTFAAGIIITLVIAFITGLFLAIGAIRFARTGRMGEAFNFSAILEKIGKIGWGSYI
                     LALIVVAVVAGVIGFILSVIPIIGILLVLIAAPALAIFSARFITLVYDSVPDAAPAAP
                     PAA"
     gene            15074..15760
                     /locus_tag="VKO45_03535"
     CDS             15074..15760
                     /locus_tag="VKO45_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HMA04977.1"
                     /translation="MDIGKVLNDSLEYAKDAVWEKWVRWLLLIIMSVIFPLIWGYRVE
                     VYRGKKPAPEPTNWVGLFVDGIKLLVIEFIYLIPVFIVLIIFIGVGVLSLIRGSPGAV
                     IAGVGSILGGILVAIIVFIIVMLFAIVGAIRFARTGRMGEAFNFSAILDRIGKIGWGS
                     YILALVVVAIVAGVIGIILAIISAIPVIGWLIALIVGPVVTILVARYVTLVYDSVPEG
                     AAPVAAPPAA"
     gene            15887..17377
                     /locus_tag="VKO45_03540"
     CDS             15887..17377
                     /locus_tag="VKO45_03540"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HMA04978.1"
                     /translation="MRFFDTTLRDGEQTPGVSLSPAEKLEIARKLSEIGVHVIEAGSA
                     AASEGEREGIRLIAGDGLSAEICTYVRALRQDIDYAADLGADSVHLVIPVSDLHIEKK
                     LRKTRDQVSHMAWEAVEYAKERGLIVELSGEDASRADQEFVRSIFSGGVERGADRLCF
                     CDTVGLLTPERAAEIVPPLAAIAPLSIHCHDDLGVAMANTLAALRAGATCAHVTVNGL
                     GERAGNTPLEELAMALEVLYGVKTGIKTGEIFHLASLVSRLTKVPLPVNKAIVGEMAF
                     THESGIHAHGILRDARTYEPIRPEMVGRKRRIVLGKHSGTASVEAALQELHYHADAHQ
                     VAVIVTRIKKLGDEGKRVTDNDLMAIAETVLGLEAKPALTLRQFTVVSGSHVVPTASV
                     TLMVNGKEITGASTGDGPVDAAISSLQRSVAEVGNIRLEEYHVDAITGGTEALVDVTV
                     RLSKDGKIITSRGASTDIIMASVEAVIAGMNRLLREEDEVRSENTH"
     gene            17349..19019
                     /gene="ilvB"
                     /locus_tag="VKO45_03545"
     CDS             17349..19019
                     /gene="ilvB"
                     /locus_tag="VKO45_03545"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HMA04979.1"
                     /translation="MKSGAKILIEGLEREGVETIFGYPGGVVLPIYDELYNAPIRHIL
                     VRHEQAAAHAADGFARASGRVGVCLATSGPGACNLVTGIATAYMDSVPIVALTGQVPT
                     NLLGNDAFQEADISGITLPITKHSYLVKDVRDLSRVVREAFFIARTGRPGPVLIDIPK
                     DVSTNQAEDKPPEGPVSLRGYQPTVKGHARQIEKALALLDAAERPVIYAGGGIIASNA
                     SAELVELAEQFMIPVTTTLMGIGCIPCDHPLNLGMLGMHGTEYANFAVTECDLLLAVG
                     ARFDDRVTGKVSTFAPHAKIIHIDVDPAEIGKNKPIDVPIVGDARSVLQAMLHKAKKG
                     ARENWMEKIRAWKKKHPLRYKEDGKLRPQFIIKELSDLLKGEGVIVSEVGQNQMWTAQ
                     WYCFRKPRSWLTSGGLGTMGYGFPAAMGATFARPGEQVFDIAGDGSFQMNIQELGTVS
                     ANKIPVKVAILNNMFLGMVRQWQELFYDRRYSYTELPPVDFVKIAAAYGVDGIRVESV
                     DEVREALKTAIATDGPFVMDFRIEREENVFPMVPAGAAINEMIGGHPR"
     gene            19016..19519
                     /gene="ilvN"
                     /locus_tag="VKO45_03550"
     CDS             19016..19519
                     /gene="ilvN"
                     /locus_tag="VKO45_03550"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HMA04980.1"
                     /translation="MKPHTLAVLVLNRPGVLSRVSGLFSRRGFNIESLAVGTCEEPDM
                     SRITIVVIGDDAQVEQVIKQLNKLIDVVKVSDITGMETVDRELALIKVTAEAGSSRAE
                     VMQIADIFRAQIVDVGSKSVVLEVTGGSTKIDALESLLRQYGVKELVRTGKISMLRGT
                     KTVKPTK"
     gene            complement(19718..>20054)
                     /locus_tag="VKO45_03555"
     CDS             complement(19718..>20054)
                     /locus_tag="VKO45_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04981.1"
                     /translation="MKRYEGLIPRVIGIIGEEGFALELEGILRKHEGLARRVRKAGRV
                     ERCAADAAGRVRATGEPPERAAAHAALAGNLSQASVYRMAGILEGEGISLSPAAILRE
                     MGRIKRGIY"
BASE COUNT         3739 a         6391 c         6308 g         3616 t
ORIGIN      
        1 ccccgccgtc ctcgaaacgt accgggagca gctccgggtg ggaagggacg agtacgcgat
       61 gaagctcgat accatcgtcc ggaaaacccc ccaggggatc tacctcgtcg aggcgggagg
      121 gccggagccc ggtgttggct gtgccgggcg cggggtactc accgccctcc agatcctcaa
      181 ggacctgaag gccttcgaga cctatggaat cgacgtcgcc atctacgacg tgctcggcga
      241 tgtggtctgc ggggggtttg ccatgcccat cagggagggc ttcgcccgcg agatctacct
      301 catctgctcc gggggattca tgagcatcta cgcggcgaac aacatcgccc gggccgtgca
      361 gcgcctcgcc cgccggggag acaccggtct ttccgggatc atctgcaata gcaacgggga
      421 cgaggccttt gagcatgcct tcgttcccga gttcgcgagg cgcctcggca ccaccttcgt
      481 ccggtttgtc ccgaggagcc cggtcatcca ggcctgtgag ctggagggga aggccgtggt
      541 ggagcattcc ccggaatcca gggaagccgg gatcttcagg gatctcgcga gggatatcat
      601 ggggaacgat tcgagggtga tcccgacgcc ggtggaggac ctcgcggagc tcgagcagct
      661 gtatcggaag taccggaagg gctgaggcgt tcgggggcta aagatccgtc tttccctccc
      721 gaggccgggc gagctccctc cccccgcgtt atcgcggtga tggggtcccc ttgatctgct
      781 cgagaaactt cgccgcgtag gcaaagagcc cgtcgttcgg gttgtagagg gggtacagct
      841 cggagaagga gaaaatggca tcgaagatgt tcgtcctcgg gaggacatcg gtgtacttca
      901 ggagatagag catcgcgatg gtcggggatc gggagacccc ggcgatgcag tggatgagga
      961 ccttttttcc gtccgccagc tgccggtgga cgaactcgag ggccgggccg atgcaccggg
     1021 tgttcacctg gtccaccttc atggcatcct cgaaggcgag gtacagctcg ttcccgccgg
     1081 gggtgaggac cacgtcctgg aagaccagcc ggtgcctttc cacgtactcg tggtgcagct
     1141 ccttctcggc atggatgacc gcccagtccg gtcttgccct cacgagttcg ttatccccga
     1201 gatccccgat gaacaggttc gggatcacct cttccatgat acccccggct ccccacgtag
     1261 tatctctttg tccagacgca atcaagcttg tgcccgaacg ggttatctcc cgaagtattc
     1321 cgggaccctt tcaggttcta ccagatcagg gagccctggg acatgggtca ctgcactccc
     1381 cccggtcctg cctcctgttt tgcccccggt ccggtccctc ccgggagaag ggaagggttc
     1441 ttcaggtgcc tggtgagaaa gtatgaaacg atcacgggag atcaacgtat ggacctgaaa
     1501 ggcagtaaga ccgagaagaa cctgctcgcc gcctttgccg gtgagtccca ggcgaggaca
     1561 cggtacagct tctttgcgag cgctgcgaag aaggagggat acgagcagat ctccgggatc
     1621 ttccaggaga cctcggacaa cgagagggag catgcccagc tctttttcaa ccacctgaag
     1681 ggcgggattg cagagatcac cgcctcctac ccggccggga tcaccgggac caccgcggcc
     1741 aacctgaagg cagccgccga gggggagaag ttcgagtggg gaaccctcta tcctggttac
     1801 ggggacgtgg ccgagaagga agggtttgcc gatgtggcaa ataccttccg gcaggtggcg
     1861 aaggtcgagg cgtatcatga gaggcgctac cggaagctcc tggaagccgt ggagaaggga
     1921 acggtctttt cgaggaaggc ccccatcaag tggaagtgcc ggaactgcgg gtatgtccac
     1981 gaggcggccg aggtcccggt gaagtgcccg gtctgcgccc atgcccgggc ctactttgag
     2041 gtctgggtcg agaactattg aaacatcacc tcctcttttt tataccaccg gtaaggggtg
     2101 ggagcggttc aaccggtatt ttcccggttt ctcgccctct cccacacgaa cctcgcggcg
     2161 gcgtacccga tggcggcgat ggtcgcgagg atgaccaggt cgaggagcag gttcacgtaa
     2221 tcctggatga taccgaggct cccgacgaag tacccgagga ttaccgtgac cccgcaccag
     2281 aggaccgctc cggccaggtt tgcgaccatg taccggccat agtccatgga ttcgaggccc
     2341 gcaaagaagg gaggaagatt gacggccggg atgaacctgc tgatcaccac cgaaagggga
     2401 ccgaaccggt caaggagcgc cctcgccctc aagatattct tcgcgctgag aatgtacggg
     2461 caggccctcc ggcagacgac gagggagaaa agcctcccgg accagtagtt caggtcatac
     2521 ccgacgtagg ctccggcaat cgaggcgacg agcacccatg cgagggacac ctggttgttc
     2581 accgccatcg ctccggcaag gaagaggatg gattgatccg gaacgggggt gaccacgaag
     2641 accgcggcga tgaggatcac caagaagacg aggagataga accagatccc cgagtgaaac
     2701 gtcgggccga ggagggatgg ggcggtatcg gggatcaggt ggagtacgct ctggagcatc
     2761 ccggttctcc tttttctccg gctctatatg aaacatcggg aggggagctt cgggaaggac
     2821 gaccgtccct tttatttcag ggaagggaaa atctccatcc atgaccatcc aggtgatccc
     2881 cgtccagggg ctccccctca tcgggaaggg tgacgacatc cccgccctcg tctgcgaacg
     2941 gatcgccttc caggacgggg acatcctcgt cctcgcctcg accatctact ccaaggccag
     3001 gggattcacg aggaggctcc aggatatcac cccttcggag agagcggaga ggatcgcccg
     3061 gagggataag gaggaccccc ggttcgtcca gtcggtcctc gacgaggcaa gggaggtact
     3121 cctggagcat cccttcattc tttcggaaat ggccttcggc cacatcggcg tacggtccgg
     3181 cgttgaccga tccaatatcg aggacgggat ggtcatcctc cttcccccgg atcccatggg
     3241 cggggccacc gagatccggg agaggatccg ttccctcgcg gggaaggagg tgggggtcat
     3301 catcaccgac acctgcggcc gggccttccg caggggacag acgggaaatg ccatcgggtg
     3361 ggcggggttt cccgccatca gggactaccg gaacgacacc gacctcttcg gccacgtcct
     3421 cctcatcacc gaggaggcgg tggtggacga gatcgccggc tttgcgaact tcgtgatggg
     3481 ggagagccac caggggattc ccgccgccgt cttccggggc tgcggccggt ggaccggcca
     3541 cgacgacctg tacttcaaac ccgccgagga tgtcatccgg aaggccatct gcacgtacgt
     3601 cggggggccg cctggtggcg ggcccgttcc cggaaaacgg tgatccctta cctccgcctg
     3661 tccacgagcc gtctcggcct cccggagagc cggaagatct cgaggctccc gggcccggcg
     3721 aacgtgaggt caaaggtgag gctcccctcg tcgtgggctc tcctgaggta gggatttttc
     3781 ccgatgaatc cccggataaa ccggtcgcgg attccctccc ggtcgatcct cgccgggtcg
     3841 agaacctcca ccgagacctg aagcaccgtc ccgccctccc ggccgcggga aatgaggaag
     3901 gactcgtact cgccgctcag gtactccatg ttctccctct ggaagacccc gcgctccacg
     3961 tcgatccggt tgaaaaacgc ccctccggcc cggaaggtct cggcgtccct cgtcgggttc
     4021 atgatccgca tgtgggtcct gccgcaggga cacctctctc tcgagatcac ggttgcggaa
     4081 tcctcggtat cgtagttgag gaggagcgtg ccgcactgtt cccccttccc tatgagggtt
     4141 gagaggatga tcctcccgca ctccccgtcg ggtacgaagg aggagagccc cgggtcgtag
     4201 atatccaggt ggacgagatc ctcgggaacg tggaggccca ggtgctgctc gcattccccg
     4261 cacatggtgc cctcggtcga cccgtaggtg ttgtgcaccg ggcagcccca gatctcctcg
     4321 acataggccc gggactccgg ggcgaaggac tcaccgccgg cgaccagcct cgcgacccct
     4381 gattccgacg gccggatccc cgcagaggag agctcccggg cgagggcaag gaacctgaac
     4441 accgatccca ccatgccggt cgggtggtag ctctccagga tcctccccgg ggaccggcag
     4501 gtgccctggg ggatgaccgt catcccgatc ccccgggcgg cgagggtcat ggtgttcgcc
     4561 cccacgttcc tcccgtacgg aaagcagatg acgacccggt ctccccggcc aaagccctgg
     4621 gagatgaaga tccgggcgta cttctcggcg taccgttccc agtcctgcca ggtgaggaag
     4681 aaggccttcg gggtgccgct cgtcccgctc gtctcgtgga cggtgaagac cgtatcccag
     4741 cccacgctcc ggaagaggaa gtcctttgtt tccggcggct ggttcgcccg gatggtattt
     4801 cccgagatga cgggaaggcc gaggaggtcc tcgtgagtct ttacccccga tggatcgatc
     4861 ccgtggccat cgaaccagga ccggtagaag ggggagttct cgtaggcgta cctgacggta
     4921 taccgcaccc tttcgtcgat gatcccgttc agggtttccc gatcggcggt ctcgatggtc
     4981 ccgctgtagt accctgatga tggcatgttc ccggggagag atggagggga gggataataa
     5041 agggaggtac ccgccctcac ggccggcggt ccttcccctc ctccggcctt gatttcctcc
     5101 acgccttcac aaggtttgcg agaaaataga tgatgagaac gaaggtggca aagataagaa
     5161 gggggtactc cagctcccgg aggacctcga tcccgaatac cgcggaaccg acgacgatga
     5221 tgaggaccgc gatcccgtag aggaggatct cgctccggat ccccttcagt acctcgagga
     5281 gaacatcccc gaagggtggc ggttccaccc tcctcgtttc cggtccgccg tgcatattca
     5341 tgattccttt tcctctctcg cagcgatctg ccgatataag tgggtttggt gttgccgata
     5401 aaaggattat gctgtggccg gtgtgaatga aaaaaagcgg ggggattact ccggcgcttc
     5461 tcctgacttc tcctggcgct tgaagctctc caggaggacg atctccccga tctcggggta
     5521 gaactcgaag atctcctgca cgacccggct ccgccagagg agccagcgcc ggtcgtacat
     5581 gatggcgggg ggaaggtgga tggtcaccac cccgttcccg acggtgacct ccatgctgcg
     5641 ctgggtgtag agctggatga gcccccggat ctgctcgggc tgggactcca cgacctcctc
     5701 gatccggtac cggtagctca cggacttccc ggcgaagggg tggttgaagt cgacgatgac
     5761 cctcctcccg atgatgttcg tcacggtccc ctcgcggttc tcgatctcca cgctctggcc
     5821 gagggagggc ttctccttga accgggccag ggacaccgac tccaccctgg cctcgtcgtg
     5881 ggctccgaag gccttctcgg gggggacctc caccgtcccc tcgtccccgg cctccttccc
     5941 gacgagggcg tcgtcgaggc cgatgaccac gtgccgcttc cccacccgga ggagcaccgg
     6001 cccgtagacc gcctcggggt tgtggatccc cgcctccttc gccaccgcct cgtcggtggt
     6061 gtcgaacacc ttttcgccaa tcttcccggt atagctcaac ctcacgaagg aaccttccgc
     6121 aacgggcatc tctgtcacct tgtctttatg aggtcgggat gaggggattt atatttaacc
     6181 cggggagaac ccggtagagg tggaggaata tccgggtgca gttcaggaca tcccggaacc
     6241 ctgaccgtca cctgatgcga atgtgaaggc cgaaagaacc gggaagatgc caattaccga
     6301 ggtgagagag gttgatggag tacgaggtaa agatccgggt tgaaggtctc gcatcgatcc
     6361 ggtcccggct cacggccctt cggatccggc cggaagggat gacgacggaa agggacctct
     6421 acctcaactc tcccacccgc gacttcggga ggaccgacga ggccctgcgg atccgggcga
     6481 ccagggacgg gacgaccctc acctacaagg ggccgaagat cggcctcggg gagatcaagg
     6541 cccgggagga gctcatcgtc ccggtggccg gcccgggcga taccctcgag aagatcctcg
     6601 tccggcttgg cttcaccccg accgccctcg tcgagaaaga ccgggagacg taccgggtgg
     6661 aggggaccct ggtcgccctc gacgaggtga aggggctcgg ctccttcgtg gagatcgagg
     6721 cccctccggg cctcggagag gccgaggccg tggccctgat ccgcagggtc agggaaatcc
     6781 tcgggatcac gggtgagaat accaccctgt catacctcga gatgatcctc gctacacggt
     6841 gaggagctcg atccggatat cccgcgattc gtctcccagg gtgacgaggg cgcagtgtcc
     6901 ctctgaggcg ggtccgggat tcaccaccat caccccgtcc tcttcggtaa gaccctttgc
     6961 ctcgtggata tgggcgcagc agaccaggtc gaaccgtttc atgtgcctcc gcagcgccgg
     7021 gcttcccacc cggttctccc cgacccggtc cagggtgccc tgcgggggtg cgtgggtgac
     7081 gaggatgttg tggacgttct tctccatcct ctccatcgcc ccctcgagga ggtccccgat
     7141 ctcctcctcg gtgagctcaa agggggttcc gaagggggtc cggttcgacc ccccgattcc
     7201 cgtcaccgtg atcttcccga gggtgatgga gcgcccgtgg atggacacgg cctccgactc
     7261 ctcgagggaa tccaggatct ccgggggatc gcagtttccg gggatcgcaa ggcagggtac
     7321 ctcgatccgc tcgaggagcc cgagggcatc ctcgcagggc ccgaactggg tgatgtcccc
     7381 gcagatgaag acgagatcgg ggtccagggc aaggaaggag tccagcttcc cgtaccggcc
     7441 gtggaggtct gcaagaagga ggacccgggt catggagaag tatcggcgtg gggcatagat
     7501 aaccttccgg ccgggtccct ccaggagccg gtgagcttcc cctcgagggc gagccgcccg
     7561 aggagccggt cggcggaccg gctcaccggg tgcggggagg tccccgcgag gggggtgccg
     7621 gggaggggga tgaaggcgtg gagacggacc cggccggaac gggtcaccca ccggatgaga
     7681 tcgagggtcg cctcctcgtc ctccggttcc tcgaagggga aacccaggat gagatcgacc
     7741 accggggtga gcccgtggtc cctgcagagc tccaccgccc gcatcccgtc cgcaacggtg
     7801 tgccccctgc cgatcttcct caagacccgg tcgctccccg actggatccc gaaggccacc
     7861 cgcgtgttgg agcagtaccg tgcgatgaga tcgagcgccg gatcggaaac gaactccggg
     7921 cggacctcgt tcgggaaagt accaaagtag atgttcccct ctagggtggc gaggagcctc
     7981 tctaccctgt caagcctcgg ccgtctcccg tccgagccat aggcgagggc gttcggggag
     8041 acgagcctca cgtcccggta ccggcgggcg tactcggcga tggtgtcgat ggaccggtgc
     8101 cgcatggagg agccgaagat gcggggggtc tggcagaagg cgcacccgaa ggggcacccc
     8161 cgggagatct cgatatagcc cttcatcacc gaaaaggggg gataggcatc caggcggacc
     8221 gcatggtccg ccgggagacc ccattccttt gtcacgacgc cgggaaccag gccctccctc
     8281 ccctcctcga tacgggtcag gagccggggg agggtgtatt ccccctcccc cactaccacg
     8341 taatccgcgt accgggcaac ttcccggggg catgcggtcg cgtgggggcc gccgacgacg
     8401 gtggtgcagg aggcgtcccg gatctccttt cggaaccggg gttcattcag ggagttgagg
     8461 gagtagcagg tgacctcgga ctccggggac ttcacggggc ggagggaaaa gccgtacagg
     8521 gagcaggccg caaacaggga ggcgtaggag ttcctggcgg cgttaatgag cctccagttc
     8581 accctcatac ctgagctccc ccgcatcccc gtccacctcc gccatcttcc cgtcctcgag
     8641 gcgcgatacg ggcacctccg gatggtcgat gagggggacc cccgcgatga tggccccgac
     8701 gacgatgatg ggctccgcct cggtgttcac catggccgcg ggtgccctcc cgttccgcgc
     8761 gagggcgtag atgacgtagg aacccacggt ggaacccttc ccgtggggaa acacgaggat
     8821 ggtcccgccg acgcgcctcc cgaacagggg gtgtcccttt tcgatgacct cccccgtttc
     8881 cggatccacc ccgcccagga aggagatggg ctccggggag acgaggaggg ggccccggcc
     8941 ccttcccccg gagagacccc tggcccggat gatcaacgac atacctaata aatctctcaa
     9001 atgcgtacta tatagtggca tggacaacac catcaccaac aacccgccgg tcgaaagcga
     9061 ggttttccag ctgaagctcg agctgcagga gatccaggcc cagatcctgg atctcaggat
     9121 gaagaacgag accctccaga aggagctatc ccaggtaaag cgggagaacg cccagctgaa
     9181 gcgcatgccc ctctttgttg cggcggtagt ggaggtgatg gaaaacgggg agatctacct
     9241 gcggcagcag gggaacaacc aggagtacat cacccaggcg agcgaggatc tccacgctgt
     9301 cctgaaaccc gggaccaagg tcgcggtgaa caacgccctc tccatcgtga agatcatcgg
     9361 gaacatctac gacgcgcggg tgagggtcat ggagctcgag gaatccccgg aggtcacctt
     9421 cgagaggatc ggcggtctca agaaggagat cgaggaggtc agggaggcgg tagagtatcc
     9481 cctcacgaag cccgaggtct tccggaggct gggggtcgag ccgcccaagg ggatcctcct
     9541 ccacggtccc ccgggaacgg ggaagaccct catggccaag gcggtggccc acgaggccca
     9601 cgccaccttc atccggatgt cagggagcga gcttgtccac aagttcatcg gggagggggc
     9661 ccagctcgtg cgggagctct tctccctcgc ccgggagagg gcgccctcca tcgtcttcat
     9721 cgatgaaatt gacgccatcg cgagccaccg gacccacgac gggacgagcg ggagtgcgga
     9781 ggtccagcgg accctcatgc agctcctcgc ggagatggac ggcttcgaca accgggggga
     9841 cgtgcggatc atggccgcga cgaaccgggt ggacatgctg gaccttgcca tcctcaggcc
     9901 cggccgcttt gaccggatcc tcgagatccc ccttcccgac tccggggcga ggagggagat
     9961 cctgaggatc cactccgccc cgatgcacct cgggaagatc gatctcgacg agatcgtgag
    10021 gctcaccgag cacaccacgg gcgccgacct ccaggccgtc tgccgggagg cgggcatgaa
    10081 cgcggtcagg agaggggcgg aagcagtgga acagggggac tacatcgagg cggtgaaaaa
    10141 ggtgaggagc gagcccccgg ccatggataa caggatgtat acgtaggtcc ggattccttc
    10201 cgaccctgat tttctctttt ttgggcgagt ggccgaacag gccgggtcag gcgcagttta
    10261 tcacggcgtc gtcgagggtg gatccgggga cgccccgcct tcccctcttc atccgggtaa
    10321 agatcccgtc gccgtacatc ccgtagagcc tgccgaggtg gctcgtcatg tggtgcatat
    10381 cggaccggta ctcccgggag tacttcctcc cgttctcggc gagcttgtgc aggtagcgga
    10441 cgaactgggg gtgggcgccg gggcaacgct cctcgagcat cctcaggaac tggtgcttga
    10501 actcgaggtc tgaaatgacc gcgaggatcc cgtaatactc gagctttgcc ctcgggatcc
    10561 ccccgtttcc ctcgatgagc acggtgatct cattcaccgc gttcacgaca tcggaggcgt
    10621 tcacccatgg ctggagagtc tgctgtttca tagagatcat ctggtggttc agggtctgca
    10681 acgctgtata aagataaatc atctcaatag tacttatagt ttgctattac tgattaatcc
    10741 atgatattta atacactatt ttctaactat aaatcattgt tacattcgtg ataagaagtt
    10801 aaactcaggt ctgatttaga ttaaatatga tattatatgt tgaaagatat ctaaatccaa
    10861 gataaatgag tctgcgtgtc aaaataaagt tttttcaacc gatatactca aatagatctc
    10921 acaatgggaa gataagtccg tcgtgacgaa acgggcggag gaagaggtgg gagaagaaga
    10981 ggtgggaaaa ggggagaagc gagcgcgtcg tcctacctga atcgcaggag catgacgaga
    11041 aggttactcc ccacgatggc aatgatgagc caggcactca cccagagcca tactccctgc
    11101 gcgaggagga aggtcgccca caggagcccg gtcaggctga tgagccacat gaagagccag
    11161 gttctcaggg acccgatcga aatcaccgct gcttcaagga attggagggg atctgtcttg
    11221 aacatgttgg attcccgcat cgatcatgaa gcctgcgggg cggctccatc ctaatgcctt
    11281 ggtccgggaa ttttcatttc atcactttcc ccggttcctc acctttgaga ggtaccttac
    11341 gaccagcccg gttttcgcgt ctgcctgctc cttctcgatc ctgaggagcc tcccgtacag
    11401 cctcctttcc cgggctgtcg ccttttccgg aagccggatc ctgaccctca cgaccaggtc
    11461 gcccgcccgg ccggagatcc tcatcccctc gccttgaatc ctcacggtcc cgtcatgctg
    11521 ggtcccggag gggatctcca ccctgatcat cctgccgtcg agggtctcca gctcaacggg
    11581 ggaccccatg accgccccgg cgggtgagac atggaccatc gtctcgagat cgtttcctct
    11641 cctcgtgaac atgtcatggg ggagtaccgt cgcctccacg tacaggtccc cgcccgggac
    11701 ctcgtgatcc ccctcttccc cgagccccgg tatcaggatg cgcatcccgg tgtcgatacc
    11761 ggccggcacg cggaccttca ccctcctcgt cgcctgggtc gccccccagc ccccgcattc
    11821 accgcagggg gactccggga cccttccttt cccgccgcac acaccgcagg ggggcgaggt
    11881 gggatccggg aatcctcctg cctccttcca tccctgcctc ccggttcccc cgcaaccggg
    11941 gcattgacgc acatcccccc cggcgctccc cgtcccggca caggaaacac accgggtggt
    12001 gtacgggacc tcgatctccc tctcgcatcc ccggatcgcc tctgcgaggg tcacgggtac
    12061 cttcaccagg atatcggagg gcgatctcgg cctgaactcg gcggaggggc cccaggactt
    12121 cccccggaag aaggaatcaa aggcgtcgcc aaacccctga aagcccccgg tgtgacggct
    12181 gccccacgga gggggagacc cccccgcata ccgcgtgtaa tcctcgtggc cgaggctgtc
    12241 ataccggttg cgttccccgg ggtccgagag cacccggtag gcctcgtgga tccgcctgaa
    12301 ccggtcccct gcctcctcct cgtggcagat atcggggtgg aacctcatcg cgagggcccg
    12361 gtaggcctgc ttgatctccc ggtccgaggc tcccctcgcc accccgagaa tttcgtagta
    12421 ggtcccgggt accccctcca gacggcacgc cccctgcggc accttcgaac ccggtggaga
    12481 ccaccctcga tcggtggtcc ccccggagga aggagcacta gtggatcttc taccgggcac
    12541 ccagatatac tattccggtc cgtatatccc ccggtctccg cggggtgaga accgcaggct
    12601 attatgtatc gggaaaccgt agtgtgccgt atgaagtggg cactcctctc ggtgtgggac
    12661 aagacgggcc tcgcaggcct cgcgcgggat ctcacttccc ggggggtcag catcctcgcg
    12721 tcgggcggga ccggaaaagc cctcggggcc gggggaatcc ccttcgtcga gatctccgag
    12781 tacaccgggt cgccggaaat gatggacggg cgggtgaaga ccctccaccc caggatccat
    12841 gccggcctcc tcggccggag ggggatcgac gatgatgcca tggccgccct cgggatccat
    12901 cccatcgatc tcctcgtggc aaacctctac cccttcgaga agatggccac acagggtctc
    12961 cccctcgctg agctcgtgga gttcatcgac atcggggggc cggccatgat aagggccgcg
    13021 gcgaagaacc acgcgagcgt cgcggtggtg gtggacccgg ccgactaccc ggtggtgtcc
    13081 gcggcggtcc agcgtggcag tttcaccggc gaggaacgcc tcgccctcgc gaagaaagcc
    13141 tttgcccgta ccgcggccta cgatggggcc atcagcaacc acctccagtc cctcgaaggc
    13201 ccccttcccc tcgtctacac cctccaggca aggaacggga gaatcctccg gtacggggag
    13261 aacccccacc aggcaggggc ggtctacggg gagacaggga tcgcgggcca ggttcctctc
    13321 caggggaggg agatgtccta caacaattac ctcgacacgg acgcgtccgt ggcactcctg
    13381 cgggagttcg acgagacctg ctgcgtctgc gtgaagcaca acaacccctg cggggttgcc
    13441 acgggcgttg acccgaaaga ggcgtatatg gccgcccggg aggtcgatcc cgattcctcc
    13501 tacgggggga tcgtggccct gaaccgcgag gtggacgccg gcaccgctga ggagatctgc
    13561 cggaccttta tcgagcttgt gatcgccccc tccttcaccg gcgatgccct cggggtcttc
    13621 cgggcgaagg agaagatgcg ggtcctcacc ctccccgatc ctgccggcgg cctcgccctc
    13681 cgatccatcg acggggggct cctcatccag agtacccccc cgtacaccga gcactggcag
    13741 gtggtgaccg agcgggatcc caccgcctcc gaactcctgg ccctgcggct cgcctggagg
    13801 gtctgcaagc atgcccggtc caatgccatc gtattcgcgg acccggtccg gaccctcggg
    13861 atcggggccg gccagacgag ccgggtggat gcggcccgga tcgccgtcat gaaggcgagg
    13921 ttccccctga agggctccgc ggtcgcctcc gacgccttcc tgcccttccc cgataccctg
    13981 gaggaggcgg cgagagccgg ggcgacggcc ctcgtccagc ccggggggtc catccgggac
    14041 cgggagtcta tcgaggcggc caaccggctg gacatggcca tgatcttcac cgggatccgc
    14101 cacttccggc actgacccgt ccgccggcga aaggagaaaa cgaatacaat attttcttga
    14161 accgtttccc tttttccccc tgtaaagggc gatttcagag ataatcttat atacaaggac
    14221 aaaaagaatc ccgtacggtg attttacatg gatatcggaa agattctgaa cgattccctg
    14281 gggtatgcca aggacgcggt atgggaaaag tgggtgaggt ggcttctcct catcatctcg
    14341 agcatcatct tccccctgat cctcgggtat gagctcgagg tctaccgggg caagaaaccg
    14401 gcccccgagc ccaccaactg gggaggactc ttcatcgacg ggatcaagct cttcatcatc
    14461 gagcttatct acctgatccc gactttcatc gtgctcttcc tgaccgtcgg ggcagccgcc
    14521 ctcgcggtca cgtccccggc cgtggccatg gccatgtggg gaaccttcgc cgccggcatc
    14581 atcatcaccc tcgtcatcgc cttcatcacg gggctgttcc tggccatcgg ggcgatccgc
    14641 ttcgcccgga cgggacgcat gggtgaggcc ttcaacttct ccgcgatcct cgaaaagatc
    14701 gggaagatcg gctggggatc ctacatcctc gccctgatcg tcgtggccgt ggttgcgggc
    14761 gtcatcggct tcatcctctc ggtgattccc atcatcggca tactcctcgt gctcatcgcg
    14821 gcaccggccc tcgccatctt ctcggcacgc ttcatcaccc tcgtctatga cagtgtgccc
    14881 gacgcggccc cggccgcacc cccggcggcc tgatatcaat cctttttttt cctaccgatc
    14941 acgcggacct ctggcagtcc tccgccgcag gagatgagca tctctttgtg cccggtctcg
    15001 cacaagggtt ccctggcctg gcttcggcca atttatatat aagcataaca agaactgcac
    15061 cctggtgaga aacatggaca tcggcaaggt tctcaacgat tccctggagt atgcaaagga
    15121 cgccgtatgg gagaagtggg tgaggtggct tctcctcatc atcatgagcg tcatctttcc
    15181 cctgatctgg ggttaccggg tcgaagtgta tcgtggaaag aaaccggctc ccgagcccac
    15241 caactgggtg ggactctttg tcgatgggat aaagctcctc gtcatcgagt tcatctacct
    15301 tattcccgtc ttcatcgtgc tgataatctt catcggggtc ggggtcctct ctctcataag
    15361 agggtctccc ggggcggtca tcgccggcgt gggctccatc ctcggcggga tcctcgtcgc
    15421 gatcatcgtc ttcatcatcg tgatgctctt tgcaatcgtc ggagcgatcc gcttcgcccg
    15481 gaccggaagg atgggagagg ccttcaactt ctccgcgatt ctcgaccgga tcgggaagat
    15541 cgggtgggga tcctacatcc tcgccctcgt cgttgttgcc atcgtcgcgg gtgtcattgg
    15601 catcatcctc gcgatcattt ccgcgatccc cgttatcggg tggctcatcg ccctcatcgt
    15661 gggcccggtc gtcacgatcc tcgtcgcccg ctacgtgacc ctcgtctacg acagcgtgcc
    15721 cgagggagcc gcaccggtag ccgcaccccc ggcagcctga tccctccctt tttttcggtc
    15781 ccctccggcc tgatccccgt ttgccgatcg gaggtaccta tttataccat gccctctcac
    15841 gttttttcgg tgcattccat gagggattgc tttattcgct gaaacgatgc gcttttttga
    15901 caccacgttg agggacgggg aacagacacc cggcgtgtcc ctttcgcctg ctgagaaact
    15961 cgagatcgcc cggaagctct ccgagatcgg cgtccacgtc atcgaagcgg gatctgcggc
    16021 agcctccgag ggcgagcggg agggcatccg gctcatcgcc ggcgacggcc tctctgccga
    16081 gatttgcacc tatgtccggg ccctcaggca ggacatcgac tatgccgccg acctcggggc
    16141 agactcggtt cacctcgtca ttccggtaag cgatctccac atcgagaaga agctccggaa
    16201 gacccgggat caggtttcgc acatggcctg ggaggcggtg gagtacgcga aggagcgggg
    16261 gctcatcgtg gagctctcgg gagaggacgc atcccgggcc gaccaggagt tcgtccggag
    16321 catcttctcg gggggggtgg aacgcggggc agaccggctc tgcttctgcg acaccgtcgg
    16381 gctcctcacc cccgaacggg cggcggagat tgtgcctccc ctcgccgcca tcgcccccct
    16441 ctccatccac tgccacgacg acctgggggt ggcgatggcg aataccctcg cggccctccg
    16501 ggccggggcg acctgcgccc atgtcacggt gaacggcctc ggagagcggg cggggaacac
    16561 tcccctcgag gagctcgcca tggccctcga ggtactctac ggagtaaaga cggggatcaa
    16621 aaccggcgag atcttccacc tcgcctccct cgtctcccgg ctcacgaagg tgccccttcc
    16681 cgtgaacaag gccatcgtcg gggagatggc cttcacccac gagagcggca tccatgccca
    16741 cgggatcctg cgggacgccc gaacctacga gcccatccgg ccggagatgg tggggaggaa
    16801 gaggaggatc gtcctcggga agcactcggg aacggcgtcc gtcgaggcgg ctctccagga
    16861 gctgcactac catgccgatg cccaccaggt cgccgtgatc gtcacccgga tcaagaagct
    16921 cggggacgag gggaagcggg tcacggacaa cgacctgatg gccatcgccg agacggtcct
    16981 cggcctcgag gcgaagcccg ccctgaccct ccggcagttc accgtcgtat ccgggtcgca
    17041 cgtcgttccc accgcctcgg tcaccctcat ggtgaacggg aaggagatca ccggggcctc
    17101 gacgggtgac gggcccgtgg acgcggccat ctcctccctc cagcgctcgg tggcagaggt
    17161 gggaaacatc cggctcgagg agtaccacgt ggacgccatc accgggggca ccgaggccct
    17221 cgtcgacgtg acggtaaggt taagtaagga cgggaagata attacatcgc gcggcgcctc
    17281 cacggacatc atcatggcat ccgtcgaggc tgtgatcgcc ggcatgaaca gactgttgag
    17341 ggaagaggat gaagtccgga gcgaaaatac tcattgaggg actagagcgg gagggcgtcg
    17401 agaccatatt cggctatccc ggcggggttg tactccccat ctacgacgag ctgtacaacg
    17461 cccccatccg gcacatcctc gtacgccacg agcaggcggc cgcccatgcg gcggacggct
    17521 tcgcccgggc gagcggccgg gtcggggtct gcctcgcgac ctcggggccg ggggcatgca
    17581 acctggtcac gggtatcgcg acggcctaca tggactcggt ccccatcgtg gccctcaccg
    17641 gccaggttcc gacgaacctc ctcgggaacg acgccttcca ggaagcggac atatcgggca
    17701 tcacgctgcc catcacgaaa cactcgtacc tggtgaagga cgtcagggac ctctcacggg
    17761 tggtcaggga ggccttcttc atcgcgagga cgggccggcc cgggccggtc ctcatcgaca
    17821 tcccgaagga cgtctccacg aaccaggcgg aggacaagcc gcccgaggga ccggtttccc
    17881 tccgggggta ccagccgacg gtgaaggggc atgcgaggca gatcgagaag gcccttgccc
    17941 tccttgacgc ggccgaacgg cccgtgatct acgcgggtgg cgggatcatc gcctcgaacg
    18001 cctccgccga gctcgtggag ctcgccgagc agttcatgat cccggtcacg acgaccctca
    18061 tggggatcgg ctgcattccc tgcgaccatc ccctgaacct ggggatgctc gggatgcacg
    18121 gaaccgagta cgcgaacttc gcggtcaccg agtgcgacct cctcctcgcc gtgggggcac
    18181 ggttcgacga ccgggtcacg ggaaaggtct ccaccttcgc cccccatgcg aagatcatcc
    18241 atatcgacgt ggacccggcc gagatcggga agaacaagcc catcgacgtc cccattgtcg
    18301 gcgatgcacg gtccgtcctc caggctatgc tccataaggc gaagaagggg gcgagggaga
    18361 actggatgga gaagatccgg gcgtggaaga agaagcaccc gctccggtac aaggaggacg
    18421 ggaagctcag gccgcagttc atcatcaagg agctctccga tctcctcaag ggcgaggggg
    18481 tcatcgtctc ggaggtcggc cagaaccaga tgtggaccgc ccagtggtac tgcttccgga
    18541 agccccggtc atggctcacc tcgggaggtc tcgggaccat gggctacggt ttccccgcgg
    18601 cgatgggggc cacctttgca aggcctggcg agcaggtctt tgacatcgcc ggggacggtt
    18661 ccttccagat gaacatccag gagctcggga ccgtctcggc gaacaagata ccggtgaagg
    18721 tcgccatcct gaacaacatg ttcctcggca tggtccggca gtggcaggag ctcttctatg
    18781 accggaggta ctcgtacacc gagctcccgc cggtggactt cgtgaagatc gccgccgcct
    18841 acggggtcga cgggatacgg gtggagtcgg ttgacgaggt cagggaggcc ctgaagacgg
    18901 cgattgccac cgacggcccc ttcgtcatgg acttccggat cgagcgggag gagaacgtct
    18961 tccccatggt cccggccggg gccgccatca acgagatgat cggggggcac ccccgatgaa
    19021 gccccacacc ctcgcggtcc tcgtgctgaa ccggcccggg gtcctctccc gggtctcggg
    19081 gctcttctcc cgccgggggt tcaacatcga gagcctcgcc gtgggcacct gcgaggagcc
    19141 ggacatgagc cggatcacca tcgtcgtcat cggagacgac gcccaggtgg agcaggtcat
    19201 caagcagctg aataaactga ttgacgtggt caaggtctcg gacatcaccg ggatggaaac
    19261 ggtggaccgg gagctcgccc tcatcaaggt aacggccgag gccgggtcca gccgggccga
    19321 ggtgatgcag atcgccgata tcttccgggc ccagatcgtg gacgtgggtt cgaagagcgt
    19381 ggtccttgag gtcacgggcg ggtccacgaa gatcgacgcc cttgaaagcc tgctccgcca
    19441 gtacggggtc aaggagctcg tccggacggg gaagatatcg atgctccggg gcacaaagac
    19501 ggtaaaacct accaaataaa ttcattattt ggaaatcctc tcacgaggcc ctcgaaatga
    19561 ggtcttcgac ctcatttctc ggacccctgc cgtggccctc gaaatacggc tttcgcccca
    19621 atttttcgga ccattgccgc ggcccacgaa ataaattcta ttttctcatg gcggtggggc
    19681 gaccatcgaa gagaagtccc cggaacccca tcttttttca ataaatcccg cgcttaatcc
    19741 gtcccatttc ccggaggatg gccgctggag agagagagat tccctcccct tcgaggatcc
    19801 cggccatccg gtagaccgag gcctgggaaa ggtttcccgc gagagccgcg tgggcagcgg
    19861 ccctttccgg cggctctccc gtggcccgga cccgtccggc cgcatcggcg gcacaccgct
    19921 ccaccctgcc cgcctttctc accctccggg ccagcccctc atgcttcctg aggatcccct
    19981 cgagctcaag ggcgaacccc tcctccccga tgatgccgat gacccgggga atgagacctt
    20041 catatctttt cata
//
LOCUS       DATGDI010000095         1911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_415963, whole genome shotgun sequence.
ACCESSION   DATGDI010000095 DATGDI010000000
VERSION     DATGDI010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415963"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VKO45_03560"
     CDS             complement(<1..564)
                     /locus_tag="VKO45_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04982.1"
                     /translation="MSQDPHSMPAGVEDPPGGNAGEKITPPFYWFFSRYLSKVELAPE
                     CEQALKGLSERGVVVYALKYRSRFNSLILHDLGLRKNIPQPLYFHGAGSLLWRPATKI
                     VRSLLRSHPADPGSTGDLDRLTRLRVSSVIYLRGSERDDAADPIAQLLSAQMALETPV
                     TIVPVAVTYGPRREKKERLLVDIFFGDQ"
     gene            complement(578..>1911)
                     /gene="ptsP"
                     /locus_tag="VKO45_03565"
     CDS             complement(578..>1911)
                     /gene="ptsP"
                     /locus_tag="VKO45_03565"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HMA04983.1"
                     /translation="RNLAGVHHETVSGIGDNAIIIARDLSPADTMQMKVEKIQGFATD
                     MGGKTSHTTIVARAYEIPAVVGIEKITTEAKTGDMVIVDGTAGAVIVNPDPEIIRRYE
                     EKKRHYETIEDSLLKHARKTATTLDKRRVLIGANIEFPEEIASAVHHGTDGIGLYRTE
                     FIYINRDRLPTEEEHFCTYKKVVESKGIRWSTIRTFDLGGDKFTSDPRLAAEMNPVMG
                     LRAIRFCLREVDLFKVQLRAILRASAYGKTKVLFPMISGMEELLETKRIFNEVKKELK
                     RDGIPYGKDVKIGIMMEVPSAALIAEDLAKEVDFFSIGTNDLIQYTLAIDRVNERVSY
                     LYEPLHPAVLKLIRQVVEAGHKAGIEVAMCGEMAGEPVYTMVLLGLGLDELSMNALVI
                     PRIKNVIRAVTVKECRKLVDRIMSFNMASEIEFFVRDYMRRKFTEDFEVAD"
BASE COUNT          329 a          582 c          599 g          401 t
ORIGIN      
        1 ctggtcgccg aagaagatat ccaccagaag gcgttctttc ttctcccggc gagggccgta
       61 ggtaaccgcc acggggacaa tggtgaccgg ggtttcgagg gccatctggg cactcaggag
      121 ctgtgcgatc gggtccgccg catcgtcgcg ctcggagccg cgcagataga tcacggagct
      181 tacacgaagg cgggtgaggc ggtcgaggtc ccccgtggac cccggatcgg caggatggct
      241 tctcagcagg gaccggacga ttttcgtggc gggcctccag aggagcgatc ccgccccatg
      301 gaaatagaga ggctgcggaa tgttcttcct caggccgaga tcgtggagga tgaggctgtt
      361 gaaccggctg cggtatttca gcgcgtaaac gacaacccct ctttcggaaa gccctttcag
      421 ggcctgctcg cactcggggg ccagttccac tttggagaga tatcgcgaga aaaaccagta
      481 aaacggaggg gtgattttct ccccggcgtt cccgccggga gggtcctcga cgccggcagg
      541 catgctgtgg gggtcctgac tcatggggcc tccggtgtca atccgctact tcgaagtctt
      601 cggtaaactt ccgccgcatg tagtccctga caaaaaactc gatctcggag gccatgttga
      661 aggacatgat ccgatcgacg agcttgcggc actccttcac cgtgacggcc cggatgacat
      721 tcttgatccg ggggatcacc agcgcgttca tgctcagctc gtcgagaccg agaccgagca
      781 ggaccatggt gtagacgggc tcgcccgcca tttccccgca catggcaacc tcgatgccgg
      841 ccttgtgccc cgcctccacc acctggcgga tgagcttgag caccgccggg tgcaggggtt
      901 cgtagaggta actgaccctc tcgtttaccc ggtcgatggc cagggtgtac tggatgagat
      961 cgttggtgcc gatgctgaag aagtccacct ctttggcaag atcctcggca atcagtgctg
     1021 ccgagggaac ctccatcatg atcccgatct tgacgtcctt gccgtagggg atgccgtcgc
     1081 gcttgagctc ctttttcacc tcgttgaaga tccgcttggt ctccagcagc tcctccatcc
     1141 ccgagatcat ggggaagagc accttcgtct tcccgtaggc gctggccctc aggatggccc
     1201 tgagctggac cttgaagagg tccacctccc tcaggcagaa ccggatggcc cgcaggccca
     1261 tgacggggtt catctcggcg gccagccgcg ggtcggaggt gaacttgtcg ccgcccaggt
     1321 cgaacgtccg gatcgtggac cagcggattc ccttcgactc gacgaccttc ttgtaggtgc
     1381 agaaatgctc ctcctcggtg ggaaggcggt cgcggttgat gtagatgaac tccgtccggt
     1441 acagcccgat cccgtcggtg ccgtgatgca cggccgaggc aatctcctcg gggaactcga
     1501 tgttggcccc gatgagcacc cggcgcttgt cgagcgtcgt ggccgtcttc cgggcatgct
     1561 tgaggaggct gtcctcgatg gtttcgtagt gccttttctt ctcctcgtag cggcggatga
     1621 tctccgggtc ggggttgacg atgacggccc ccgccgtccc gtcgacgatg accatgtccc
     1681 ccgtcttggc ctccgtcgtg atcttctcga tcccgacaac agccgggatc tcgtacgccc
     1741 gggccacgat ggtggtgtgg gaggtcttgc cgcccatgtc ggttgcgaat ccctggatct
     1801 tttccacctt catctgcatg gtatcggcgg gagaaagatc tcgggcaatg atgatggcgt
     1861 tatcgccgat cccggagacg gtctcgtgat ggacccccgc caggtttctc a
//
LOCUS       DATGDI010000096        11207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_328728, whole genome shotgun sequence.
ACCESSION   DATGDI010000096 DATGDI010000000
VERSION     DATGDI010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 11207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11207
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328728"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..212)
                     /locus_tag="VKO45_03570"
     CDS             complement(36..212)
                     /locus_tag="VKO45_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04984.1"
                     /translation="MIGIEKCIDFLDRQGLQVLKGNELEIHPFTSHGACRRVPAQIRG
                     KNGMDKTIRLLQRL"
     gene            complement(769..1935)
                     /locus_tag="VKO45_03575"
     CDS             complement(769..1935)
                     /locus_tag="VKO45_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMA04985.1"
                     /translation="MRQYGSARARAIPPSGIRRFFDLLLTMEDVISLGVGEPDFETPW
                     CVAEAGIYAIEQGHTSYTSNKGILPLREAVARDLATRYGISYDPEEEIVITSGVSEAA
                     DIAIRALVDPGDEVLVAEPSYVSYSPCVTLAGGKPVPVSCLEKDEFRLTPGCLMEKVT
                     RKSRVLVLNFPNNPTGGIMGTDEINAIADVVVDHDLLVISDEVYSELTYEGSHVAVAS
                     HPELRERTVTMNGFSKAYSMTGWRIGYLCAPRPISELALKIHQYVMLCAPIMGQMAAV
                     EALKNGEEEKNAMVREYRHRRNFFVEGLNRIGLPCHVPKGAFYAFPSVAKTGLSDQEF
                     AERLLTEKQVAVVPGSVFGETGTDHLRCAYAVSRENLGTALARMGDFLSGLRGA"
     gene            complement(1943..2434)
                     /locus_tag="VKO45_03580"
     CDS             complement(1943..2434)
                     /locus_tag="VKO45_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HMA04986.1"
                     /translation="MDEKDHEILHQLDEDGRLPARELGAGIGLSEREVEERVRALEES
                     RVIRKFSTVIDWERAGNSRVSAIIDLKIAPERDFGYDKIAERIARFRQVKALYLVSGV
                     YDLQILVTGKDMNEVSRFVSEQIAPMDHIRETATHIIMKIYKENGIPFFEREGGDRLP
                     LSI"
     gene            complement(2439..3716)
                     /locus_tag="VKO45_03585"
     CDS             complement(2439..3716)
                     /locus_tag="VKO45_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HMA04987.1"
                     /translation="MPEDTSSMGLFQKYLVNKNIFRNREVLRHSYRPISLPHRQPEIN
                     SIASILAASLKGETPSNILIYGKTGTGKTACVRYVAAELEKASQETVIPCHVIHLNCE
                     VIDTQYRVLAQVAKSLEDHDERPSDGTRGTIPFTGWPTDQVYMELKNLLESVGGVMVI
                     VLDEIDKLVKKSGDETLYNLTRINSDLKDSRVSIIGISNDLRFTDFLDPRVLSSLSEE
                     EIVFPPYNAPQLCDILQQRGEDAFMENVLDPAVIPLCAALAAQEHGDARRALDLLRVS
                     GELAERGDTDKVTEKHVKMAQEKIETDSMIECVSTLPTQSKVVLYSMLLLEHIGKKIF
                     TSGEVLQVYREVAGNLDVDVLTNRRITDLVSELSMLGVINTRVVSKGRYGRTKEMWFD
                     MTSERIWEVLSKDARLSRDTLIQLDTNRLKAIR"
     gene            4330..4827
                     /locus_tag="VKO45_03590"
     CDS             4330..4827
                     /locus_tag="VKO45_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HMA04988.1"
                     /translation="MNPEHLRELTVTAAVITVSSSRTPETDESGQAAKNLLHEAGIEV
                     TYYALVPDRIEHIRTALVLALDLASCTILTGGTGLTPDDCTIEAVRPFLEKEIEGFGE
                     LFRSLSHREIGTAAMLSRALAGISRGRAVFCLPGSVAAVTLGTRELILPEIRHILSHA
                     APDQR"
     gene            complement(4818..5333)
                     /gene="cgi121"
                     /locus_tag="VKO45_03595"
     CDS             complement(4818..5333)
                     /gene="cgi121"
                     /locus_tag="VKO45_03595"
                     /inference="COORDINATES: protein motif:HMM:NF011465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Cgi121"
                     /protein_id="HMA04989.1"
                     /translation="MTTEPFEIRTAIFRVDDCSAFLGRLRAIGRARGTHIVCFNADLM
                     AGRAHAEAAVRRAFRAVAAGTVISDSFEMEALLFASGNRQCTIAQQFGIHEGENRAYV
                     CLCPPVEAAWRDLSVFILFVDRDWETLSGEKEARLREIFGITEAELGAAGAARLQDLV
                     LERVALLEVYR"
     gene            complement(5330..7483)
                     /locus_tag="VKO45_03600"
     CDS             complement(5330..7483)
                     /locus_tag="VKO45_03600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HMA04990.1"
                     /translation="MEINSLPIPPYLSDAYRGQGITELYPPQEECVRAGLFDGKNLLV
                     AIPTASGKTLVAEMAMHHHVARGGKCLYIVPLKALASEKLGEFSGKKVRVGISTGDYD
                     RREEYLGRNDIIIATSEKVDSLMRNDTPWLSELTLLVVDEVHLIDSEDRGATLEMVIT
                     KLRSRNPSMQVIGLSATIGNPRTLAAWLAAVPVTSTWRPVELREGVFYRGAIRFPDGE
                     RKVEGVSRFDDLNLCLDTVAEGGQCLVFVSSRRNAEAFAKRAAAALKRVSPALVASAR
                     ELTDLAETEMDRSLAACVERGAAFHHAGLRREQRRIVEAGFRAGEIKCISSTPTLAAG
                     LNLPARRVVIRDYLRYRAGEGMAPIPVREYHQMAGRAGRPRLDPYGEAVLIAKDRDAV
                     QDLTCAYIDAPPEDVHSQCGSATAICTHILSLVATGFTPTGARVMDFLDRTFYVHENR
                     GRQALTGVVDRALSFLSRSEMVVRLGDDLSATELGSLVSRLYLDPRGAEAIASSLVQR
                     EVFTGIGLLHLLCSTPDMPTLYLRQSDLPLLERYLYEHEDALWLPLPSLGDEDCEAYY
                     RALKTALVLADWTEEVGEAMICERYGINPGDIYSFVESLQWLVHAAARLSSRFAPHLA
                     GPVRDMETRVRSGVKEELLPLVALRGIGRVRARRLFNNGITGPDAIRGAGVDRVASII
                     GRGIAEQVFAQLGRTPREPAVGDGQAELGRFGDGS"
     gene            complement(7639..8181)
                     /locus_tag="VKO45_03605"
     CDS             complement(7639..8181)
                     /locus_tag="VKO45_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HMA04991.1"
                     /translation="MTREEVRVSQDRIGVLIGKAGATKKSLEEKTGSRISVDSKEGLV
                     AVDGEDAEGVISAADVVRAIGRGFSPERAFVLLQDKDLILDVIELTALADTPQQMDRI
                     RGRVIGKDGRSREQIEDMTGTTISVHGKTVAIIGSVEQLKTARTAVEMLLNGVPHETV
                     FAFLDRKKREAKQDLLGSYY"
     gene            complement(8258..9034)
                     /locus_tag="VKO45_03610"
     CDS             complement(8258..9034)
                     /locus_tag="VKO45_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HMA04992.1"
                     /translation="MEREPDERGFDRRIAEFGIRIKDADQWKVQDDVFDEVTLLALYR
                     LAHKKRLTTFGGPISTGKEGNVFYGEKDGLPLAIKIYMIRTANFRAMTEYIDGDPRFS
                     HIRRSRKEIIFAWTRKEFSNLKRARKAGVPVPEPYSFDRNILIMEFLGEGQKPFPQIR
                     DVEIPEPGKTYLEIIGFIETLFSRARLVHGDLSEYNILYGRGPHIIDMGQAVTPDHPR
                     ALHFLVRDIKNINRFFSDKCEVMGEREIFQRIAGERFAGP"
     gene            complement(9042..9998)
                     /locus_tag="VKO45_03615"
     CDS             complement(9042..9998)
                     /locus_tag="VKO45_03615"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HMA04993.1"
                     /translation="MTTPGTDPFQLATRNTVEVVTEEDLRRTLGMERPRCYAGYEPSG
                     EIHLGHLVTVNKLMDLQAAGFHVVVLLADLHAFLNRKGTLEEVEELSRYNRACFEALG
                     LAGVEYVTGSALQLCPEYEILVLRLAQQITLNRAQRSMDEVGRQMEHPTVSQMIYPIM
                     QMVDIAQLKADVAVGGIDQRKIHMLAREYLPPAGFPAPVCIHTPILLGLDGEKMSSSK
                     GNYISVVEKEEDIRRKLSKAFCPPTIGENPVLQILELHVFPRMPSVTVKRPAKFGGDR
                     TFETYGEMAAAYGRGEVHPMDLKNTTAEYLVEILRPVRERLG"
     gene            complement(10039..11058)
                     /locus_tag="VKO45_03620"
     CDS             complement(10039..11058)
                     /locus_tag="VKO45_03620"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HMA04994.1"
                     /translation="MIRVAINGYGTVGKRVADAVAAQPDMEIVGVSKTRPSFEAYIAK
                     QRGFPLYVADLAKRAAFEKLGLPVAGAVPEMLARADLVVDATPGGVGKENKPLYEKAK
                     VKAIFQGGEDHEVAGFSFCSDCNYKMALGRQFVRVVSCNTTGLCRIINTLDREYGIAK
                     VRAVMVRRASDPGEIKKGPVDAVVLDPVTVPSHHGPDVQTVLAHIPISTMAMIVPTTF
                     MHMHAIQVELKKDGKKERILELIEKHPRMGLVKGGLGIKSTAELKEYALDLGRPRGDL
                     WESCIFADSVAVQGRDVSFFQAIHQEADIVVENVDAIRAMMGNVKDPDESIRMTNEAL
                     HFTAI"
BASE COUNT         1884 a         3595 c         3616 g         2112 t
ORIGIN      
        1 acaggaatac gactatcggg ggagaattcc ctgaatcaaa gcctttgaag gaggcgaatc
       61 gtcttatcca tcccattctt ccccctgatc tgggctggta ctctccggca ggctccatgg
      121 gaagtaaagg ggtggatttc aagctcattt cctttcagga cctgtaagcc ctgcctgtcc
      181 agaaaatcaa tgcatttttc gataccgatc attccctcct caataatttg gtaaatacta
      241 tcttgatggc gaggtggatg tcaccggatg aacaatccgg tggttgcatg tcctccaatg
      301 gaagtaaggg ggttgtttct ggcgaatatt tcttattcga tatatacttt cctttttaag
      361 gggatcgcgt acctggccgc ccttattttt cctggttcaa ttcacgccgg aatcagattc
      421 ggatctcccc ttcggacccc cggttgtccg atccggtgcc tccagatgct ccaatggaaa
      481 taagggggta tattcccagg aatttttgaa tataaacttt ccttcatctc tcaaaatctg
      541 aaaacatcca gccagaactt ctccggcggc ccgaggggtg ggatgtcccc gtccccggtg
      601 ttcggggggg tccgccccac cctgcctcat ctcaaggggg ctttccgtct ccggcgggtc
      661 attttttcac gggaaggaat gtccccggat atccaatccg gtggttgcat gtcctccaat
      721 ggaaataaag gggttggagc ggcctttttc cgtcggagaa gaggggcctc aggcgcccct
      781 gaggccggag aggaaatcgc ccatccgggc aagggccgtg cccaggttct cccgggagac
      841 cgcgtaagca caccggaggt gatctgtccc ggtctcgcca aagaccgacc cgggaaccac
      901 cgcgacctgc ttctcggtga ggagcctctc cgcaaactcc tggtccgaaa gcccggtctt
      961 tgccaccgag gggaaggcat aaaaggcccc cttgggaacg tggcagggaa ggccgatccg
     1021 gttcaggcct tccacgaaga agttgcgccg gtgccggtac tcccggacca tggcgttctt
     1081 ctcctcctcc ccgtttttca gggcctccac cgcggccatc tggcccatga tgggtgcgca
     1141 gagcatcacg tactggtgga tcttgagggc gagctcgctg atgggcctcg gggcacagag
     1201 gtacccgatc cgccaaccgg tcatggagta ggccttcgag aagccgttca tggtcacggt
     1261 cctctcccgg agctcggggt gagacgccac cgcgacgtga gatccttcgt aggtgagctc
     1321 ggagtaaacc tcgtcggaga taacgaggag gtcatggtcc accaccacgt cggcgatggc
     1381 gttgatctcg tccgttccca tgatcccgcc cgtggggttg ttcgggaagt tcaggacgag
     1441 gacccggctc ttccgggtga ccttctccat caggcacccg ggggtgagcc tgaactcgtc
     1501 cttttccagg caggagaccg ggaccggttt tccccctgca agggtgacgc agggggagta
     1561 ggagacgtac gagggctcgg ccacgaggac ctcgtccccc gggtcgacga gggcccggat
     1621 ggcgatgtcg gccgcctccg agaccccgct cgtgatgacg atctcctctt cggggtcgta
     1681 ggagatgccg tagcgggtgg cgagatcccg ggccacggcc tcgcggaggg ggaggatgcc
     1741 cttattcgag gtgtaggagg tgtgcccctg ctcgatggcg tagatccccg cctcggccac
     1801 gcaccaggga gtctcgaagt cgggctctcc cacgccgagg gagatgacgt cctccatggt
     1861 gaggaggagg tcgaagaacc gccggatgcc cgagggcggg atggcacgcg cccgggccga
     1921 gccgtactgc ctcatggtgc ccctagatgc tcagggggag acggtccccg ccctccctct
     1981 cgaagaaggg gatcccgttc tccttgtaga tcttcatgat gatgtgggtc gcggtctccc
     2041 ggatgtggtc catgggcgcg atctgctcgg agacgaaccg ggagacctcg ttcatgtcct
     2101 tcccggtgac gaggatctgg aggtcgtaga cccccgaaac gaggtagagg gccttcacct
     2161 gccggaaccg ggcgatacgc tcggcgatct tgtcgtaccc gaagtcccgc tcgggggcaa
     2221 tctttaagtc gatgatggcc gagacccggg agttccctgc ccgttcccag tcgatgacgg
     2281 tgctgaactt gcggatgacc cgggactcct ccaacgcccg gacgcgctcc tccacctccc
     2341 tctcggaaag gccgatcccc gcaccgagct ccctcgcggg gagccggccg tcctcgtcga
     2401 gctggtggag gatctcatgg tctttttcat ccatgggatc acctgatggc cttcaaccgg
     2461 ttggtatcca attgtataag ggtatcccgg gagaggcgag catccttgga gaggacctcc
     2521 cagatgcgct ccgaggtcat gtcgaaccac atctccttgg tcctcccgta ccggcccttg
     2581 gaaacgaccc gggtgttgat caccccgagc atcgagagct cggagacgag gtcggtgatc
     2641 cgccggttcg tgagcacgtc cacatccaga ttgcccgcca cctcccggta gacctggagg
     2701 acctcccccg aggtgaagat cttcttcccg atatgctcga ggaggagcat ggagtagagg
     2761 accaccttcg actgggtggg gagggtcgag acgcactcga tcatgctgtc ggtctcgatc
     2821 ttctcctggg ccatcttcac gtgcttctcg gtcaccttgt ccgtgtcgcc ccgctcggcg
     2881 agctcccccg agacccggag gaggtcgagg gccctccggg catccccgtg ctcctgggcg
     2941 gcgagggccg cgcagagggg gatgacggcg gggtcgagga cgttctccat gaaggcgtcc
     3001 tcgccgcgct gctggaggat gtcgcagagc tgcggcgcgt tgtagggagg gaagacgatc
     3061 tcctcctcgg agagggagga gaggaccctc gggtccagga agtcggtgaa ccggaggtcg
     3121 ttcgagatgc cgatgatgga gacccgggag tccttgagat ccgagttgat ccgggtcagg
     3181 ttgtagaggg tctcgtcccc gctcttcttc acgagcttgt cgatctcgtc gaggacgatg
     3241 accatcaccc cgcccacgga ctcgagcagg ttcttgagct ccatgtacac ctggtcggtg
     3301 ggccacccgg tgaaggggat ggtgccgcgc gtcccgtcgc tcggccgctc gtcatgatcc
     3361 tccaggctct tcgcgacctg ggcgaggacc cggtactggg tgtcgatgac ctcgcagttc
     3421 aggtggatga cgtggcaggg gatgaccgtc tcctggctcg ccttctcgag ctcggcggcg
     3481 acgtaccgga cgcaggcggt ctttcccgtc ccggtcttcc cgtagatcag gatgttactc
     3541 ggggtctcgc ccttaaggga agcggcgagg atggaggcga tggagttgat ttcaggctgc
     3601 cggtggggga ggctgatcgg ccggtacgag tggcggagca cctcccgatt cctgaagatg
     3661 ttcttgttca cgagatattt ctggaacagt cccatggacg aggtgtcttc aggcataatc
     3721 tcaggtgaag aattgcgctc cggtgatatg gccccggccc ctcttaagct gatcgttttc
     3781 tccctgttta ttaacccctc atcttcctgt ggaaatattg caatatttcc ctttttccac
     3841 aaaataaccc ctataatcgt taatcttgag agatggtttc catgccccga acctctttgc
     3901 cctgtccatc cagctcgacc cccctgagag gctgatgatg gagggggcac ccggagtttc
     3961 acctggcggg ggaatttccc ggccccctcc cccctttgct tcctgtggag atgatgaaaa
     4021 tagcctgttc ctgtactggt attttcagaa gaaatagccg gttgtttctc tgtttcattc
     4081 catataaaaa tagatcgaaa tgaacaggaa gcatggtaaa gggcgaagga aggaacttgt
     4141 gcagactctt aggggttccc tgagggaatt cccgacggaa accgggcccg gccctcaggg
     4201 gaacccccgg tctcctctcc actggaaata agggggtccc gggtcgagag aacaggtaca
     4261 gtgaccgcag aaggggaacc ctggcatttc ctttatcttt ccccgggcca atcatgagct
     4321 cggagaggca tgaaccccga acacctgcgg gagctcaccg tcacggccgc ggtcatcacc
     4381 gtctccagca gccggacccc ggagaccgat gagagcgggc aggccgcgaa gaacctcctt
     4441 cacgaggccg gcattgaggt gacctattac gccctcgtcc ccgaccggat cgagcatatc
     4501 cgcaccgccc tcgtcctcgc cctggacctc gcctcctgca ccatcctcac cggaggcacc
     4561 ggtctcaccc ccgatgactg taccatcgag gcggtccggc ccttcctcga gaaggagatc
     4621 gaggggttcg gggagctctt ccgctccctg tcccaccggg agatcgggac cgcggccatg
     4681 ctctcccggg ccctggccgg gatctcccgg ggacgggcgg tcttctgcct ccccggttcc
     4741 gttgcggcgg taaccctcgg cacccgggag ctcattcttc ccgagatccg gcacatcctc
     4801 tcccacgcgg ccccggatca gcgatagact tcgaggaggg caacccgttc caggaccaga
     4861 tcctggagtc gcgccgcccc ggccgccccg agctcggcct ccgtgatccc gaagatttcc
     4921 cggagccgcg cctctttctc tccggagagg gtctcccagt cccggtccac gaacaatata
     4981 aagacagaga ggtcacgcca ggcggcctcc acgggagggc acaggcagac gtacgcccgg
     5041 ttctccccct cgtggatacc gaactgctgt gcgatagtgc actggcggtt cccggaggcg
     5101 aagaggaggg cctccatctc gaaggagtcc gagatgaccg tccccgccgc gaccgcccgg
     5161 aatgcacgcc tgaccgcggc ctccgcgtgg gcccggcccg ccatgagatc cgcgttgaag
     5221 cagacgatgt gcgtcccccg ggcacgcccg atggcccgga gcctgccgag gaacgcggag
     5281 cagtcgtcca cccggaagat cgcggtgcgg atctcaaagg gttcggtggt catgagccat
     5341 ccccgaaccg gccgagctcg gcctgcccgt ccccgactgc gggttcccgc ggggtcctgc
     5401 cgagctgggc aaagacctgc tcggcgatgc ccctgccgat aatggaggcg acgcggtcca
     5461 cgcccgcccc gcggatggcg tcgggcccgg tgatcccgtt gttgaagagc cgccgggccc
     5521 ggaccctgcc gatgccccgg agggcgacca gggggaggag ctcctccttg acgccggacc
     5581 tgacccgggt ctccatgtcc cggaccgggc cggcgaggtg gggggcgaac cgggaggaga
     5641 gccgggccgc ggcatggacg agccactgga ggctttcgac gaaggagtag atgtcccccg
     5701 ggttgatccc gtaccgctcg cagatcatgg cctcccccac ctcctcggtc cagtcggcga
     5761 ggacaagggc ggtcttcagg gcccggtaat aggcctcgca gtcctcgtct cccagggagg
     5821 ggagggggag ccacagggca tcctcgtgct cgtagaggta ccgttcgagg agggggagat
     5881 ccgactggcg gaggtagagg gtgggcatgt cgggtgtcga gcagaggagg tgcaggagcc
     5941 cgatgccggt gaagacctcc ctctggacga gggaggaggc gatggcctcc gcgcccctcg
     6001 ggtccaggta gaggcgggag accaggctcc cgagctccgt ggccgagaga tcgtccccga
     6061 gccggaccac catctcggac cgcgaaagaa aggagagtgc ccggtccacg acccccgtga
     6121 gggcctggcg accccggttc tcgtggacat agaaagtacg gtccaggaag tccatgaccc
     6181 gcgccccggt gggggtgaag cccgtggcga cgagggagag gatgtgggtg cagatggccg
     6241 tcgcagaccc gcactgggaa tggacatcct cgggcggggc atcgatgtag gcgcaggtga
     6301 gatcctgtac cgcatcccga tccttcgcga tgaggaccgc ctccccgtac gggtccaggc
     6361 gggggcggcc ggcccggccg gccatctggt ggtactcccg caccgggatg ggggccatcc
     6421 cctcgccggc ccggtaccgg aggtagtccc ggatgacgac ccgccgggca gggaggttga
     6481 ggccggcggc gagggtcggg gtcgaggaga tgcatttgat ctccccggcc cggaacccgg
     6541 cctccacgat cctccgctgc tccctccgga gccccgcgtg gtgaaaggcc gccccccgct
     6601 ccacgcaggc cgcgagggac cggtccatct cggtctcggc gaggtccgtg agctcccggg
     6661 ccgaggcgac gagggccggg gagaccctct ttaaggcggc cgcggccctc ttcgcgaagg
     6721 cctcggcatt gcgccgggag gagacaaaga ccaggcactg acccccctcg gccacggtgt
     6781 ccaggcagag gttcaggtcg tcgaaccggg agacgccctc gaccttccgt tccccgtcgg
     6841 ggaaacggat ggcaccccgg tagaacaccc cctcccggag ctccaccggc cgccaggtgg
     6901 aggtcaccgg caccgcggcg agccaggcgg cgagggtcct cgggttcccg atggtcgcgg
     6961 agaggccgat gacctgcatc gaggggttcc gggagcgaag cttcgtgatg accatctcga
     7021 gggtcgcccc ccggtcctcg gagtcgatga ggtggacctc gtccaccacg aggagggtga
     7081 gctccgagag ccagggggtg tcgttcctca tgagggagtc caccttctcg gaggtggcga
     7141 tgatgatgtc gttcctcccc aggtactcct cgcgccggtc gtaatcccct gtggagatgc
     7201 cgacccggac cttcttcccc gagaactccc cgagcttctc gctcgcgagg gccttcagcg
     7261 gaacgatgta caggcacttc cccccccggg cgacatggtg gtgcatcgcc atctcggcaa
     7321 cgagggtctt tccgctcgcc gtggggatgg ccacgaggag gttcttgccg tcgaagaggc
     7381 cggcccggac gcactcctcc tggggaggat agagctcggt gattccctgg ccccggtagg
     7441 catccgagag gtagggggga atggggaggg agtttatctc cattcagagc ccccataccc
     7501 gacggaaagc cggtgccccg gtccggatag atctgcggtg cccggtacct gctggccaga
     7561 ccattctcgc cgcgcgataa cccgtctccc gccgggggtc ccgccccctc cgggaccgtc
     7621 tctccctcac ccgaccccct aatagtacga accgaggagg tcctgcttcg cctcccgctt
     7681 cttccggtcg aggaaggcaa agaccgtctc gtgggggacc ccgttcagga gcatctccac
     7741 agcggtcctg gccgtcttca gctgctcgac gctcccgatg atggcgacgg tctttccgtg
     7801 gaccgagatg gtggtcccgg tcatatcctc gatctgctcc cgcgaccgcc cgtccttccc
     7861 gatgacccgg ccccggatcc ggtccatctg ctggggagtg tcggcgaggg cggtaagctc
     7921 gatgacatcg aggatcaggt ccttgtcctg gaggaggaca aaggcgcgtt cgggtgaaaa
     7981 acccctcccg atggcgcgga caacatcggc cgcggagatg accccctcgg catcctcacc
     8041 atccacggcg acaaggccct ccttgctgtc cacgctgatc cgggaccccg tcttctcctc
     8101 gaggctcttc ttcgtcgctc ccgccttccc gatgagaacc ccgatccggt cctgggagac
     8161 cctgacctcc tctcgtgtca tctcttcatc ccggagatcc tcacggcccg gcgaacctct
     8221 caccggccct tacgggccgt tgatatctca ccggccctta tggcccggcg aacctctcac
     8281 cggcgatccg ctggaagatc tcccgttcac ccatgacttc gcacttgtcc gagaaaaacc
     8341 tgtttatgtt cttgatatcc cgaacgagga agtggagggc ccggggatgg tccggggtca
     8401 ccgcctgtcc catgtcgatg atatggggcc ccctcccgta caggatgttg tactcggaga
     8461 ggtcgccgtg gacgagccgg gcgcgggaga agagggtctc gatgaaaccg atgatctcaa
     8521 ggtaggtctt cccgggctcc gggatctcca cgtccctgat ctggggaaag ggcttctggc
     8581 cttccccgag gaactccatg atgaggatgt tccggtcaaa ggagtacggc tcggggacgg
     8641 ggacccctgc cttcctcgcc cgcttaaggt tggagaactc cttccgggtc caggcgaaga
     8701 tgatctcctt ccttgaccgc cggatgtggg agaaccgggg atccccgtcg atgtactcgg
     8761 tcatggcccg gaaatttgcc gtccggatca tgtagatctt gatggcaagg ggcaggccgt
     8821 ccttctcccc gtagaagaca ttcccctcct tccccgtgga gatgggcccg ccgaaggtgg
     8881 tgagccgctt cttgtgcgcg agccggtaga gggcgaggag ggtgacctca tcgaagacgt
     8941 cgtcctgcac cttccactgg tccgcgtcct tgatccggat cccgaactcc gcgatcctcc
     9001 ggtcgaaccc ccgctcgtca ggctcgcgct ccacggggcc ctcaccccag gcgttcgcgc
     9061 accgggcgga ggatctccac caggtactcc gccgtggtgt tcttcaggtc catgggatgg
     9121 acctcccctc tcccgtaggc cgcggccatc tccccgtagg tctcaaaggt ccggtccccg
     9181 ccgaacttcg cgggcctctt caccgtgacc gacggcatgc gggggaagac gtggagctcg
     9241 aggatctgga ggaccgggtt ctccccgatc gtaggggggc agaaggcctt cgagagcttc
     9301 cgccggatgt cctcctcctt ttcgaccacc gagatgtagt tccccttcga ggaggacatc
     9361 ttctccccgt cgagcccgag gaggatgggg gtgtggatgc agacgggggc cgggaagccg
     9421 gccgggggaa gatactcgcg ggcgagcatg tggatcttcc gctgatcgat gccccccacg
     9481 gcgacatcgg ccttcagctg cgcgatgtcc accatctgca tgatggggta gatcatctgg
     9541 gagacggtgg ggtgctccat ctgccggccc acctcgtcca tggaccgctg ggcccggttg
     9601 agggtgatct gctgggcgag ccggagcacc aggatctcgt actcggggca gagctggagg
     9661 gcggatccgg tgacgtactc cacgccggcg agcccgaggg cctcgaagca ggcccggttg
     9721 taccgggaga gttcctccac ctcctcgagc gtccccttcc ggttcaggaa ggcgtggagg
     9781 tcggcgagga ggaccacgac gtggaacccg gcggcctgga ggtccatgag cttgttcacg
     9841 gtgaccaggt gcccgaggtg gatctccccg ctcggctcgt agccggcata gcagcggggc
     9901 cgctccatcc cgagggtgcg ccggaggtcc tcttcggtca ccacctcgac ggtattccgg
     9961 gtggcgagct ggaagggatc cgtcccgggg gtcgtcatgt agaggaaaat aggcggcggg
    10021 ggtggtaaaa ggtttctttc agatggccgt gaagtgaaga gcctcgttgg tcatccggat
    10081 ggactcatcc gggtctttca cgttccccat catggcccgg atggcgtcca cgttctccac
    10141 gacgatgtcg gcctcctggt ggatggcctg gaagaaggag acgtcccttc cctgcaccgc
    10201 cacggagtcg gcgaagatgc agctctccca gaggtcgccc ctcggccggc ccaggtccag
    10261 ggcgtactcc ttcagctcgg cggtggactt gatgccgagg ccccccttca cgagccccat
    10321 cctcgggtgc ttctcgatga gctccaggat ccgttccttc ttcccgtcct tcttgagctc
    10381 gacctggatg gcgtgcatgt gcatgaaggt ggtcgggacg atcatggcca tcgtgctgat
    10441 ggggatgtgg gcgaggaccg tctggacatc gggcccgtgg tggctgggga cggtcaccgg
    10501 gtcgaggacc accgcgtcca ccggcccctt cttgatctcg cccgggtccg aggcccggcg
    10561 caccatcacg gccctcacct tggcgatgcc gtactcccgg tcgagggtgt tgatgatccg
    10621 gcagaggccg gtggtgttgc aggagacgac ccggacgaac tgcctcccca gggccatctt
    10681 gtagttgcag tccgagcaga aggagaagcc ggcgacctcg tgatcttccc cgccctggaa
    10741 gatggccttc accttcgcct tctcgtagag gggcttgttc tcctttccca ccccgccggg
    10801 ggtcgcgtcc accacgaggt cggccctggc gagcatttcc gggaccgccc cggccacggg
    10861 gagcccgagc ttctcgaagg cggcccgctt cgcgaggtcg gcgacataga gggggaaccc
    10921 ccgctgcttc gcgatgtagg cctcgaagga ggggcgggtc ttcgacaccc cgacgatctc
    10981 catgtccggc tgggccgcga ccgcgtccgc cacccgcttc cccacggtgc cgtacccgtt
    11041 gatggcaact cgtatcatga ttgatgaatg gggagagggg cttttaaggt tttttacatt
    11101 catcccggga cacggcccgg atgatcgtcc aggtaatccc tctggatcgc gaccacctcg
    11161 ccgctgtccc ccgccccggc ataccgttcg aggggctcgc ggttcac
//
LOCUS       DATGDI010000097         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_183429, whole genome shotgun sequence.
ACCESSION   DATGDI010000097 DATGDI010000000
VERSION     DATGDI010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183429"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VKO45_03625"
     CDS             complement(<1..634)
                     /locus_tag="VKO45_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HMA04995.1"
                     /translation="MNPSPLADILCSWDLQFKAEHPEVALAGSPERSLFRTVIEDAGA
                     KRFVLEKIDPRMVDRKREIAAAVDNLASRGLAGVHPYLRNRDGDFITKAAGGFWQVSP
                     FIEGVELRRPEYTLEAWRGKALARFLIDMKEKSEGIPFGKPGRPFSIAEFTRDLMTKM
                     SRHNPERAAAVQKPYHHVEKHLFPAHDRLPVAFCHGDPHPINVIWGDSDIR"
     gene            complement(682..1089)
                     /locus_tag="VKO45_03630"
     CDS             complement(682..1089)
                     /locus_tag="VKO45_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04996.1"
                     /translation="MRKFARTLTLAFSAGALGGLVNSLCVWLAVKYGLTAALGVSLAA
                     PLSPAWIYPRIVWGGIWGLLFVVPLWERNPFLRGLFFSIGPTLVMLFVIFPLRLGKGM
                     MGLDLGTWTPAFVILFNAAWGWTAALWMRWVNR"
     gene            complement(1086..1688)
                     /locus_tag="VKO45_03635"
     CDS             complement(1086..1688)
                     /locus_tag="VKO45_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HMA04997.1"
                     /translation="MPPAAHDPEADREFFRQAMEGARPLTGSNREEGRGKPSPAGLAP
                     KSAGEEALEGMKALIASGRGFVVADTPEYIEGTGYQVDPKMAERLHRGEFSIQAHIDL
                     HGLGAEEARVAFEDFLRESVIRNRRSLLVIHGRGLSSQGEPVLKARLTEWLTRGPWRK
                     WVIAYSSARLCDGGAGATCILLRTRPVTKRHKKDPSRERP"
     gene            complement(1842..>2229)
                     /locus_tag="VKO45_03640"
     CDS             complement(1842..>2229)
                     /locus_tag="VKO45_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA04998.1"
                     /translation="VKLQRRMSVFAKISQRSDDAASEDMPGLDESKMEKAMEMIARES
                     GGLDENDPRQAAQLMRKLTEATGLSLGPKMEEALARMEKGEDPEKIEAEMGDSIGEDE
                     LFIMAKKAASGKKDKKPRVDEKLYDL"
BASE COUNT          367 a          719 c          687 g          456 t
ORIGIN      
        1 cgcggatatc gctgtcaccc cagatgacat taatggggtg ggggtcaccg tggcaaaagg
       61 cgacgggaag acggtcatgg gcggggaata aatgcttctc cacatggtgg taaggcttct
      121 gtacggcagc ggcccgttca gggttgtggc ggctcatttt cgtcatgaga tcgcgggtga
      181 actccgcaat cgaaaagggg cgccccggct tgccgaaggg gatgccctcc gatttttcct
      241 tcatgtcgat gagaaaccgg gccagggcct ttccccgcca ggcctcgagg gtgtattcgg
      301 ggcgcctgag ctccacgcct tcgatgaagg gcgatacctg ccagaaaccg cctgcggcct
      361 tcgtgatgaa atccccgtcc cggttcctga gataaggatg gacgcctgcc agtccgcggg
      421 aggccaggtt atcgaccgct gcggcaatct cccgtttccg atcgaccatc cggggatcga
      481 tcttctcgag gacaaaacgc tttgcgcccg cgtcttcgat caccgtgcgg aacagcgacc
      541 gctccggact cccggccagg gccacttccg ggtgctcggc cttgaattgc aggtcccagg
      601 agcaaaggat atccgcaagc ggagaaggat tcacctatcg ctcccccgtt ttcgaccgaa
      661 tagacggaac agaccgccgc gtcatcgatt cacccacctc atccacagcg ccgccgtcca
      721 gccccaggcg gcgttgaaga ggatgacgaa ggcgggtgtc catgttccca ggtcgaggcc
      781 catcatcccc ttgcccagcc tcagtgggaa gatcacgaag agcatgacca gggtcgggcc
      841 gatgctgaaa aaaagccccc ggagaaaggg gtttctctcc cagaggggga cgacgaacag
      901 aagcccccat atcccgcccc atacgatgcg cgggtagatc catgcagggc tcaggggggc
      961 ggcgaggctc acgccgaggg ccgccgtgag gccgtacttc acggcaagcc agacacagag
     1021 gctgttgacg agtccgccca gcgccccggc gctgaaagcg agggtcaggg ttcttgcaaa
     1081 ttttctcatg gccgctccct ggaggggtct tttttgtgtc gtttcgtaac gggccttgtc
     1141 cgcaggagga tgcacgtcgc cccggcgccg ccgtcgcaga gcctggcgct cgagtaggcg
     1201 atgacccact ttcgccaggg cccgcgggtg agccactcgg tcagcctggc cttcagaacg
     1261 ggctctccct gcgaggagag gccccggccg tggatgacga gcagcgagcg ccggttgcgg
     1321 atgaccgatt cccgcaggaa atcctcgaaa gcgacgcggg cctcctcggc gccgaggccg
     1381 tgaaggtcga tgtgggcctg gatggagaac tcgccccggt gcagccgctc ggccatcttc
     1441 gggtccacct ggtatcccgt cccctcgatg tactcgggcg tgtcggcgac gacgaatccc
     1501 cggccggagg caatgagcgc cttcatccct tccagcgctt cctcgccggc gctcttcggc
     1561 gccaggcctg ccggggaagg ttttcccctg ccctcttccc ggttcgagcc tgtcaggggg
     1621 cgggcgccct ccatggcctg gcggaagaat tccctgtccg cctcggggtc atgagcagcg
     1681 ggcggcacga tcggctcccc gatcccctgc cgcttctttt cttggggctt ctcttgggtc
     1741 ttttctttga agcgtccctt gaggtccttg aaagggttgt gaccgagggg cgtccgaggc
     1801 gattccattg tcctcaccac tgggctgaag ccgtctccga ttcacagatc gtagagtttc
     1861 tcatcgaccc ttggtttttt gtcctttttt cccgaggctg ctttttttgc catgatgaac
     1921 aactcatctt cgccgatgct gtcgcccatc tctgcctcga tcttttccgg gtcctcccct
     1981 ttttccatcc gggcaagggc ctcctccatc ttcggcccga gggagagccc cgtggcttcg
     2041 gtgagtttcc gcatcagctg cgccgcctgg cgggggtcgt tctcgtcaag cccgccggat
     2101 tcccgggcga tcatctccat cgccttttcc atctttgact cgtcgaggcc gggcatgtct
     2161 tcggacgcgg catcgtcgct tctctgagag atcttcgcga agaccgacat ccggcgctgg
     2221 agcttcact
//
LOCUS       DATGDI010000098         3585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_125290, whole genome shotgun sequence.
ACCESSION   DATGDI010000098 DATGDI010000000
VERSION     DATGDI010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125290"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..915
                     /locus_tag="VKO45_03645"
     CDS             331..915
                     /locus_tag="VKO45_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate synthase family protein"
                     /protein_id="HMA04999.1"
                     /translation="MDEEGFGMLGRMMVALGEIEGCREFAGLIPEVRTNLVYARSLAK
                     TRDDVLAVDGRITVVEGRPRAAGRVKFGASSHMARLIIELRKVDPSVRAGIDFAWTPH
                     LSEWLQAYCRERGWVYSVIDRRGEPEEIKEAEGASMPWKVAEAVRAAGGKVPKIFYET
                     GAVGKEPVSVLVGRDPIEVAGQACEIARRYHGGG"
     gene            902..1945
                     /locus_tag="VKO45_03650"
     CDS             902..1945
                     /locus_tag="VKO45_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HMA05000.1"
                     /translation="MAAGNRKIGKIDRETFESFILARLGKPDASVIVPPRTGVDAGVI
                     DLEDGRVLIVAEDPIFAIPGQPPEMFGWYTVHIGASDVAVMGVPPRYMTYTLLMPPGT
                     ADPEFRAIVDAIHGAAVDLRIAIVGGHTGYYPGFAAPTIGGVTVFAVTARDRYVTPAG
                     ARPGNAVILTKGPAIETAGILSVLRERELLARYPEALVRRAQGLGRQMTVVRDALTAM
                     AAGGVTAMHDATEGGVIGGLAEVAEASGVGMVVDEARFVYPDEVRMVCEAFGIDPAAA
                     IAEGSLVITSEASRAPLIIGDLRKAGIAASAIGMVTGNTGSRVMIRRDGSEVPLRMPA
                     QDPFWPVFFESVG"
     gene            complement(1990..3108)
                     /gene="thiI"
                     /locus_tag="VKO45_03655"
     CDS             complement(1990..3108)
                     /gene="thiI"
                     /locus_tag="VKO45_03655"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HMA05001.1"
                     /translation="MTDLWLVRYSEIFLKSDYVRREWERALMRNIREVLPGCRVRSER
                     GRIWVGGEVDPEKLRRVFGIVSFSPVTRCTLENLNETLLDLCRERGILDLHSFAIRMR
                     RVGVHSFTSQEKAVELGNLVRREFPGLEVDLDHPDIELHVEIREGDCYLYTGVIRGAG
                     GLPLGVEGSLVALVSGGIDSPVAAWMMMKRGCRILPVFIALEGFLDDDARERAEKVVE
                     VLRNYQPDIRLRVVPDAYLACARRELEGMDLERFTCLLCKRRMYRIAEAMAREEGAKG
                     IVTGESLGQVASQTLENLLVLDDAATLPVYRPLIGLDKEEITRIAREIGTFETSTKAV
                     GGCRAVPGKPSTRAELGKIREIEESLRCNREIPVRKNP"
     gene            3349..>3585
                     /gene="nadA"
                     /locus_tag="VKO45_03660"
     CDS             3349..>3585
                     /gene="nadA"
                     /locus_tag="VKO45_03660"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HMA05002.1"
                     /translation="MEKNEALVKGIQALREERDAIILAHNYQVGPVQDIADLVGDSLE
                     LSRAAASMEGKVIVFCGVDFMAETAAILSPEKKVL"
BASE COUNT          596 a         1155 c         1242 g          592 t
ORIGIN      
        1 ccgttcctcg agacgatcac cccggcgggg ttgatgtcca gggcgaggag cacctcagag
       61 gccctggccg catctccctc gtaggtccgc acggtcccgt cctggagctg gatcttcatg
      121 ggatgctcag atcccattct cgccccggcc ctttaaaatc ccctgccatg ggcccgggag
      181 ccccgttcca acggcatttt ccggaggatg cgggggtccc ttcgccggtc gggaaagggg
      241 aaccggacgt tcataggact ggagggaggg agcatttttt gtaggccggc ctccctatgc
      301 tctagcggag aggccggggc aggtgaggcg atggacgagg aaggattcgg gatgctcggg
      361 aggatgatgg tcgcgctcgg ggagatcgag ggatgccggg agttcgccgg actgatcccc
      421 gaggtgcgga cgaacctcgt gtacgcgagg tccctcgcaa agacccggga tgacgtgctc
      481 gccgtcgacg gccggatcac ggtcgtggag ggcaggccga gggcggcggg ccgggtgaag
      541 ttcggggcct ccagccacat ggcacgcctg atcatcgagc tcaggaaggt ggacccctct
      601 gtccgggcgg ggatcgattt tgcctggact ccgcacctct cggaatggct gcaggcctac
      661 tgcagggaga ggggatgggt ctactcggtc atcgaccggc gcggcgagcc cgaggagata
      721 aaagaggcgg aaggagcctc catgccctgg aaggtggccg aggcggtgcg ggcggccggc
      781 gggaaggtgc cgaagatctt ctacgagaca ggcgccgtgg gaaaggagcc ggtctcggtc
      841 ctcgtgggaa gagatccaat tgaggtggcg gggcaggcct gcgagatcgc gaggaggtac
      901 catggcggcg ggtaacagga agatcgggaa gatcgaccgg gaaaccttcg agtccttcat
      961 cctcgcccgg ctcgggaaac cggacgcgag cgtcatcgtc cctccccgga ccggcgtcga
     1021 tgcgggggtg atcgacctgg aggacggccg ggtgctcatc gttgccgagg accccatctt
     1081 cgccattccc gggcagccgc cggagatgtt cgggtggtac accgtccaca tcggggcgag
     1141 cgacgtggcc gtcatggggg tgcctccccg gtacatgacc tacaccctgc tcatgccccc
     1201 cggaaccgca gatccggaat tccgggccat cgtcgatgcc atccacgggg ccgccgttga
     1261 tttgaggatc gccatcgtgg gggggcacac cgggtactac ccggggttcg cggcgcccac
     1321 catcggcggg gtgaccgtct ttgccgtcac ggcccgcgac aggtatgtga cacccgcggg
     1381 ggcgaggccg ggcaacgcgg tgatcctcac gaaggggccc gccatcgaga ccgcggggat
     1441 cctctcggtt ctccgggagc gggagctcct cgcccggtac ccggaggccc tcgtccggag
     1501 ggcccagggc ctcggccggc agatgaccgt cgtccgggat gccctcaccg cgatggcggc
     1561 cggcggcgtg accgcgatgc acgacgccac cgaggggggc gttatcggcg ggctcgccga
     1621 ggtcgcggag gcgagcggcg tgggaatggt ggtggacgag gccaggttcg tgtaccccga
     1681 cgaggtcagg atggtctgcg aggccttcgg gatcgacccg gccgcggcca tcgccgaggg
     1741 ctccctcgtc atcacctccg aggcgagccg ggcccccctc atcatcgggg atctcaggaa
     1801 agccgggatc gccgcctctg ccatagggat ggtaacgggg aataccggtt cccgggtgat
     1861 gatccggcgg gacgggagcg aggtgcccct ccggatgccg gcccaggacc ccttctggcc
     1921 ggtctttttc gagagtgtcg ggtagggatc ccgccccttt tcaggagagg cgggaggatc
     1981 acccgggcat caggggttct tccggaccgg gatctccctg ttacacctga ggctctcctc
     2041 gatctcccgg atcttcccga gttctgccct cgtggagggc ttccccggga ccgcccggca
     2101 cccccccacg gcctttgtcg acgtctcgaa ggtcccgatc tcccgggcga tccgggtgat
     2161 ctcctccttg tcgaggccga tgaggggccg gtagacgggg agggtcgccg cgtcgtcgag
     2221 caccaggagg ttctcgaggg tctgggaggc gacctggccc agggactcgc cggtgacgat
     2281 cccctttgcc ccctcctccc ttgccatggc ctccgcgatc cggtacatcc tccgcttgca
     2341 gagcaggcag gtgaacctct ccaggtccat cccctcgagc tccctcctcg cgcaggcgag
     2401 ataggcgtcc gggacgacgc ggagccggat gtccggctgg tagttccgca gcacctccac
     2461 caccttttcg gccctttccc gtgcgtcgtc atcgaggaag ccctcgaggg cgatgaagac
     2521 cgggaggatc cggcacccgc gcttcatcat catccatgcc gcgaccggcg aatcgatccc
     2581 cccggatacc agggcgacaa ggctcccctc caccccgagg ggaaggccgc ctgccccccg
     2641 gatcaccccg gtgtacaggt agcagtcccc ttcccggatc tcgacatgga gctcgatgtc
     2701 cgggtggtcc aggtccacct ccaggccggg gaattcacgg cggacgaggt tcccgagctc
     2761 caccgccttt tcctgcgagg tgaaggaatg gacccccacc cgccgcatcc ggatggcaaa
     2821 gctgtggaga tcgagaatgc cgcgctccct gcagagatcg aggagggtct cgttcaggtt
     2881 ctccagggtg cagcgggtaa ccggggagaa ggagacgatg ccaaagaccc gcctgagttt
     2941 ctccgggtcg acctcccccc cgacccagat cctccccctc tcggagcgaa ccctgcagcc
     3001 cgggagcacc tcgcggatat tcctcatgag ggccctctcc cattcgcgcc ggacatagtc
     3061 cgacttcagg aagatctcgg agtagcgtac cagccagagg tcggtcatgg agatcgggat
     3121 cccgctcttc cagagattgc ccctccggca ttatggcgct ttcctctcct cagtgacctc
     3181 ccgggacaag gacgtacagg cagctggtca gggccgggtg gatttgagga gttcctgtga
     3241 cactcggagg gcaaccctta tacgatgatg accaaaggtt aagaactgaa aagaccgtga
     3301 caggagcggg gtctgccgag ccgggagtgg cccggaggag aggtgcccat ggagaagaac
     3361 gaggcgctcg tcaagggaat ccaggcgctc agggaggagc gtgacgccat catcctcgcc
     3421 cacaactatc aggtcgggcc ggtgcaggac atcgccgacc tcgtcgggga ctccctggag
     3481 ctctcccggg cggctgcctc catggagggg aaggtcatcg tcttctgcgg cgtggacttc
     3541 atggccgaga cggccgccat cctctcgccg gagaagaagg tcctc
//
LOCUS       DATGDI010000099         6386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_212758, whole genome shotgun sequence.
ACCESSION   DATGDI010000099 DATGDI010000000
VERSION     DATGDI010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212758"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VKO45_03665"
     CDS             <1..924
                     /locus_tag="VKO45_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HMA05003.1"
                     /translation="ERDPEHLFRIIVLNPDTGHGRAGGVRLRSPLHNLYFMRDQQAAT
                     DRGICMGRMATPERSEEVGVSSLCLAALGIEPVHRVDEGRFEGGDFMPAGEFALVGCG
                     SRTNPEGVRDILGGGLGFDEVGVVSEPRHPLISGRDPMVNMHLDTYLNFPNEGVAVGN
                     PDLLREAKVTVYHREGGGYTRAGAAGTLPEYLTGKGFDIIPITTLEQLCYSTNFLCVR
                     GGACITPDASTLAPIVLKRLGEKAQDHPAKYGRLLSQAEHDYQNLRNESEFFPHKREV
                     YAHGLEMTTLLLTNATGGYGGAHCMTCVLAR"
     gene            complement(926..1981)
                     /locus_tag="VKO45_03670"
     CDS             complement(926..1981)
                     /locus_tag="VKO45_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HMA05004.1"
                     /translation="MRLVVALGGNAILRRGEKGTAEEQLGHVRYAAANLTALVKGGHR
                     IAITHGNGPQVGDILLKNECAKDTLPRMPLDICGAESQGMLGYLIQQCLENALRDAGI
                     DRRVITVVTQTVVDPADPAFSRSSKPLSGAFSQTPRAPGPGGPSKPLNGAASQTLRAL
                     GPGGPTKPIGPFYTGAEARRLAEKEGWVLHPVEGGLLRRVVPSPAPVRIVEESSIRTL
                     VEEGFVVIAAGGGGIPVVEGASGNLGGVEAVIDKDLTAERLAAAIGAEVLLILTDVEH
                     VSLHYGTPDAEPIREAHAPKIRAWLAEGHFPEGSMGPKMLACARFVEEGGRCAIVTSL
                     EKALDGLEGRAGTRIVP"
     gene            2097..3392
                     /locus_tag="VKO45_03675"
     CDS             2097..3392
                     /locus_tag="VKO45_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HMA05005.1"
                     /translation="MFWDEKAETLKGKALGALQLRRLKGTVAQAGKVPFYRKLLGDAG
                     VGPGDIRTLADVKRLPFTGKKDLRGGYPFGFLAVPLREVRRIHTTSGTTGKPTVVGYT
                     GKDLSNWAGLIARNLTMVGLGEGDVFQNMVNYGLFTGGLGFHYGAELMGLTVVPSATG
                     NTRRQIEMIRDFGVTAIHCTPSYAMHLSEVMEEMGARLPTLKTGCFGAEPWSEATRGE
                     LERRLGVSAFDSYGLSEMYGPGVAMECPEKDGLHIWHDSFLVEIIDPATGENLGPGEK
                     GEMVITPLVKEAMPLLRYRTGDITMLMDEDGCSCGRGQKISRILGRSDDMLVVRGINV
                     FPSQIEHVLFSLPEVGEQFMVYLDRIHQLDEMTIEVEMNRGAFSGELRDLHLIQERIA
                     RALRETLGIRTDVRLVEPGSLPRFEGKAKRVVDRRGVLF"
     gene            3389..4690
                     /locus_tag="VKO45_03680"
     CDS             3389..4690
                     /locus_tag="VKO45_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HMA05006.1"
                     /translation="MMYWDSKMETMEKEELGRLQYRLLKTLVYRLYSFSPFYHRRMEE
                     AKVHPDDVKTLSDVTKLPFMFKRDLRDNYPDKLFTATKEELVRYHVSSGTTGKPTVVG
                     YTENDLEEWTVSLARALTSCGLGRKDVIQVSYGYGLFTGGLGLHYGAERIGATVIPTS
                     VGGTERQIELMQDLGATAICCTPSYLLHMGEVAEKMGVSIKKDTKLRTGILGAEPWTA
                     EMRNRIQDWLGIKAYDIYGTSELSGPLFTECTEQSGIHVWGDFAYTEIVDPKTGEQVA
                     DGEKGELTMTILKKEALPIVRYRIGDITSMTREVCPCGRTHPRISRITGRVDDMLIIR
                     GINVFPSMIEHTLMGIPEVGQHFQIVVDRVHELDSLLVRVEMKPEAFSDKITDLMKVK
                     EMIGRRLESALSVHADVELAEPGSLPRFEGKAKRVIDRRKM"
     gene            4690..5124
                     /locus_tag="VKO45_03685"
     CDS             4690..5124
                     /locus_tag="VKO45_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HMA05007.1"
                     /translation="MEHERYFIKQISVFSENKPGRLAAIAKAMEEEGVNIFAFSIAEA
                     DGFGVVRALVNHPDKAYRKLTSLGFVVSFTDVIGVKMRDEPGGLYEVARLLGDANINI
                     EYSYAYSGKEAAVLILRVDSIRNAIEKIVGAGGSLMEAAFFR"
     gene            complement(5181..>6386)
                     /locus_tag="VKO45_03690"
     CDS             complement(5181..>6386)
                     /locus_tag="VKO45_03690"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HMA05008.1"
                     /translation="GGADWVEAGTPLIKSAGMEAVRRLRAEFPDREIVADMKVADTGT
                     LEVEMAAKSGAGVICILATADDEVVREAVRAKNLYGVRIMADLINSPDPVGRAFELEE
                     LGVDIIAAHTGIDQQMVGLTSVELARELGGRLSVPLAVAGGLDAGSAAEAVRSGASII
                     IVGGNIIRSGDVTGSTRRIRKAIDRPGERTSPVRTRDEEIRELLMGVSTPNISDAMHR
                     KGAMRDILSICGDVKMAGKAVTVQTFAGDWAKPVEAIEIAGPGDVLVINNDGGVHVAP
                     WGELATRSCMGKGVSGVVIDGAVRDVDDIRRLSYPLFARAIVPNAGEPKGFGEINAEI
                     RCCGEVVRPGDWIVGDMNGVVVIPRERAYEVARRAREVQKNEERIREEISRGGTLSVI
                     QELEKWEKR"
BASE COUNT         1093 a         2146 c         2109 g         1038 t
ORIGIN      
        1 gagcgggacc cggagcacct cttccggatc atcgtcctga acccggacac cgggcatggc
       61 cgggcgggcg gggtgcggct ccgctccccc ctccacaacc tctacttcat gcgggatcag
      121 caggcggcca ccgaccgggg aatctgcatg gggaggatgg caacccccga gcgatcggag
      181 gaggtagggg tatcctccct ctgcctcgcc gccctgggaa tcgagccggt ccaccgggtg
      241 gatgagggga ggttcgaggg aggggacttc atgcccgcgg gggagttcgc cctcgtcggc
      301 tgcggatcgc ggacgaaccc ggagggtgtc cgggatatcc tcgggggagg gctcggcttt
      361 gacgaggtcg gcgtcgtgag cgagccccgg catcctctca tcagcggccg ggaccccatg
      421 gtgaacatgc acctggacac ctacctgaac ttcccgaacg agggggtggc agtcgggaac
      481 ccggacctcc tccgggaggc gaaggtgacg gtctaccacc gggaaggcgg aggctacacc
      541 cgggcggggg ccgcgggaac cctgccggaa tacctcacgg gcaagggttt cgatatcatc
      601 cccatcacca ccctcgagca gctctgctac agcacgaact tcctttgcgt taggggcggt
      661 gcctgcatca ccccggatgc gagcaccctg gctcccatcg tgctcaaacg ccttggcgag
      721 aaggcgcaag accacccggc aaagtacggg aggctcctct cccaggccga gcatgactac
      781 cagaacctca ggaacgagtc cgagttcttc ccccacaagc gggaggtcta tgcccatggc
      841 ctggagatga cgaccctcct ccttacgaac gccacaggag ggtacggcgg ggcccactgc
      901 atgacctgcg tcctggcccg gtgaatcacg ggacgatgcg ggtccccgcc ctcccctcca
      961 gcccgtccag ggctttttca agggaggtga cgatggcgca cctgccgccc tcctcgacga
     1021 accgggcaca cgccagcatc ttcggcccca tggagccctc cgggaagtgc ccctccgcga
     1081 gccatgccct gatcttcggt gcgtgggctt cacggatcgg ttccgcgtcg ggtgtcccgt
     1141 aatgcaggga tacatgctcg acgtcggtga ggatgaggag gacctccgcc ccgatggccg
     1201 ccgcgaggcg ttcggcggtg agatccttgt cgatgaccgc ctcgaccccg cccaggttcc
     1261 cggatgcgcc ctctaccacc gggatgcccc ctccgcccgc ggcgatgacc acgaatccct
     1321 cctcgacgag ggtccggatg gaagactcct cgacgatccg gacgggggcc ggcgagggga
     1381 ccacccgccg gagcaaccct ccctcgaccg ggtggaggac ccagccctcc ttttcggcga
     1441 gccgcctcgc ctctgccccc gtatagaagg gcccgatggg cttcgtgggc cctcccgggc
     1501 caagggcccg cagggtctga gaagccgctc cgttgagcgg tttcgagggc cctcccgggc
     1561 caggagcacg gggggtctga gaaaacgctc cgctgagcgg cttcgaggag cgggagaagg
     1621 ccgggtcggc ggggtccacc acggtctggg tcaccaccgt gatgacccgc cggtcgatcc
     1681 ccgcgtcccg gagggcgttc tccaggcact gctggatgag gtacccgagc atcccctggc
     1741 tctctgcccc gcagatatcg aggggcatcc gggggagggt gtcctttgca cactcgtttt
     1801 tgaggaggat atcccccacc tgggggccgt tcccgtgagt gatggcgatg cggtgccctc
     1861 ccttcacgag cgccgtgagg ttcgccgccg cgtaccgcac gtggccgagc tgctcctcag
     1921 cggtgccctt ctccccccgg cggaggatgg cgttcccgcc cagggcaacg acaaggcgca
     1981 tagggaagtg gtggggtggc ccggggattt aataggtagg tgggcaggcc ggaaagttcg
     2041 ttccgaatca tgcaccataa atagcatgcc atgcaacggc atatcgggga ttccggatgt
     2101 tctgggacga gaaggcggag accctgaagg ggaaggccct cggggccctc cagctccggc
     2161 ggctcaaagg gacggtcgcg caggccggaa aggtcccctt ttaccggaaa ctcctcggtg
     2221 atgcaggcgt cggcccgggc gacatcagga cccttgccga cgtgaagaga ctccccttta
     2281 ccggcaagaa ggacctccgg ggggggtacc ccttcggatt cctcgcggtt ccgctgaggg
     2341 aggtccggcg gatccacacc acctccggga ccacgggaaa gcccaccgtc gttggctaca
     2401 cgggaaagga cctctcgaac tgggccggcc tcatcgcccg gaacctgacc atggtgggcc
     2461 tcggcgaggg ggacgtcttc cagaacatgg tgaactacgg cctctttacc ggcggcctcg
     2521 ggttccacta cggggccgag ctcatggggc tcaccgtggt tccctcggcg acggggaaca
     2581 cccggcggca gatcgagatg atccgggact tcggggtgac cgccatccac tgcacgccca
     2641 gctatgccat gcacctctcc gaggtcatgg aggagatggg ggcccggctg ccgaccttaa
     2701 agaccgggtg cttcggggcc gagccctggt ccgaggcgac ccgcggggaa ctcgagcgcc
     2761 ggctcggggt ctctgccttc gattcgtacg gcctctccga gatgtacggg cccggggtcg
     2821 ccatggaatg cccggagaag gacgggctcc acatctggca tgactccttt ctcgtcgaga
     2881 tcatcgaccc ggcgacgggc gagaacctgg gcccggggga gaagggcgag atggtgatca
     2941 ccccgctcgt gaaggaggcg atgcccctcc tccggtaccg gacgggagat atcaccatgc
     3001 tcatggacga ggacgggtgc tcctgcggtc ggggccagaa gatcagccgg atcctgggga
     3061 gatccgacga catgctcgtg gtccggggga tcaatgtctt cccctcccag atcgagcatg
     3121 tgctcttctc gctcccggag gtcggcgagc agttcatggt ctacctcgac cggatccacc
     3181 agctggacga gatgaccatc gaggtggaga tgaaccgggg ggccttctcg ggcgagctcc
     3241 gggacctgca cctcatccag gagaggatcg cgagggccct gcgggagacc ctgggaatcc
     3301 ggaccgacgt gaggctcgtg gaaccgggct ccctcccccg gttcgagggg aaggcaaagc
     3361 gggtggtgga caggagaggg gtgttgttct gatgtactgg gattccaaga tggagacgat
     3421 ggagaaggag gagctcgggc gcctccagta ccggctgttg aagactctcg tgtaccggct
     3481 ctacagcttc tcaccgttct accaccggag gatggaggag gcgaaggtgc acccggacga
     3541 cgtgaagacc ctctcggacg tgacgaaact ccccttcatg ttcaagcggg acctcaggga
     3601 caactacccg gacaagctct tcacagcgac gaaggaggag ctcgtccggt accacgtctc
     3661 ctcgggcacc accgggaagc ccacggtggt gggctacacc gagaacgacc tcgaggagtg
     3721 gaccgtctcc ctcgcccggg ccctcacctc ctgcggcctc gggaggaagg acgtcatcca
     3781 ggtgagttac ggctacggcc tcttcacggg cggcctcggg ctccactacg gggccgagcg
     3841 gatcggggcc acggtcatcc cgacctccgt cggcgggacc gagcggcaga tcgagctcat
     3901 gcaggacctc ggggccacgg ccatctgctg caccccctcc tacctcctcc acatgggcga
     3961 ggtcgccgag aagatggggg tctccatcaa gaaggacacg aagctccgga ccgggatcct
     4021 cggagccgag ccctggaccg ccgagatgcg gaaccggatc caggactggc tcgggatcaa
     4081 ggcctatgac atctacggga cgagcgagct ctcggggcct ctcttcaccg agtgcaccga
     4141 gcaaagcggg atccacgtct ggggcgactt cgcgtacacc gagatcgtcg acccgaagac
     4201 cggcgagcag gtcgccgacg gggagaaggg ggagctcacc atgaccatcc tcaagaagga
     4261 ggccctcccc atcgtccggt accgaatcgg ggacatcacc tccatgacga gggaggtctg
     4321 cccctgcggg aggacccacc cgaggatctc gcgcatcacg ggaagggtcg acgacatgct
     4381 catcatccgg gggatcaacg tcttcccgtc catgatcgag cacaccctca tgggcatccc
     4441 cgaggtggga cagcacttcc agatcgtggt ggaccgggtc cacgagctcg attccctcct
     4501 cgtgcgggtc gagatgaagc ccgaggcctt ctcggacaag atcaccgatc tcatgaaggt
     4561 gaaggagatg atcggccggc ggctggagtc ggccctctcg gtccatgccg acgtggaact
     4621 cgccgagccg ggctccctcc cgaggttcga gggaaaggca aagagggtga ttgacaggag
     4681 gaagatgtaa tggagcatga gaggtatttc atcaagcaga tctcggtctt ctcggagaac
     4741 aagccgggga ggctcgcggc catcgcgaag gccatggagg aggagggggt gaacatcttc
     4801 gccttctcca tcgccgaggc ggacggcttc ggggtggtgc gggcgctcgt gaaccacccg
     4861 gacaaggcgt accggaagct cacctccctc gggttcgtcg tgtccttcac cgatgtcatc
     4921 ggggtgaaga tgcgggacga gccgggcggg ctctacgagg tggccaggct gctcggcgat
     4981 gcgaacatca acatcgagta ctcgtacgcc tacagcggga aggaggcggc ggtgctgatc
     5041 ctccgggtgg acagcatcag gaacgccatc gagaagatcg tcggggccgg cggatccctc
     5101 atggaggcag ccttcttccg gtgatcatcg caccgcttcg cgttgctcca gctccgttcc
     5161 cctgaaggag aacgtcaccc tcacctcttc tcccacttct cgagctcctg gattaccgag
     5221 agggtgcccc cccgggagat ctcctcccgg atccgctcct cgttcttctg cacctcccgg
     5281 gccctcctcg ccacctcgta ggcccgttcc ctcgggatga ccaccacccc gttcatgtcc
     5341 ccgacgatcc agtcccccgg gcggacgacc tcgccacagc accggatctc ggcgttgatc
     5401 tccccgaagc ccttgggctc gccagcgttc gggacgatgg cccgggcgaa gagcgggtag
     5461 gaaagccgcc ggatgtcgtc gacatccctc accgcgccgt cgatgacgac cccggacacc
     5521 ccttttccca tgcagctcct cgtggcgagc tccccccagg gggcgacgtg gactcccccg
     5581 tcgttgttga tgacgagaac gtcgcccggc cccgcgatct cgatggcctc cacgggcttt
     5641 gcccagtcgc ctgcaaaggt ctggacggtg accgccttcc ccgccatctt cacgtcgccg
     5701 cagatggaga ggatatcccg catcgccccc ttccggtgca tggcatccga gatgttcggg
     5761 gtggacaccc ccatgagcag ttcgcggatc tcctcgtccc gcgtgcggac gggactcgtg
     5821 cgttccccgg gccggtcgat ggcctttcgg atcctccggg tcgagccggt cacgtcgccg
     5881 gaccgtatga tgttcccgcc gacgatgatg atcgaggccc cggatctcac cgcctccgcc
     5941 gccgatcccg cgtcgaggcc tcccgcgacg gcgaggggga ccgagagccg ccccccgagc
     6001 tccctcgcga gctccacgga ggtgagcccc accatctgct ggtcgatccc cgtgtgggcc
     6061 gcgatgatgt ccacccccag ctcctcgagc tcgaaggccc ggccgaccgg gtccggcgag
     6121 ttgatcaggt cggccatgat cctcactccg tagaggttct tcgcccggac cgcctcccgg
     6181 accacctcgt catccgcggt ggcgaggatg cagatgacgc cggctcccga ctttgcggcc
     6241 atctccacct cgagggtgcc cgtgtccgcg accttcatgt cggccacgat ctcccggtcc
     6301 gggaactcgg cccggagcct ccggaccgcc tccatcccgg cgctcttgat gaggggggtc
     6361 ccggcctcta cccagtcggc cccgcc
//
LOCUS       DATGDI010000100        20874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_241963, whole genome shotgun sequence.
ACCESSION   DATGDI010000100 DATGDI010000000
VERSION     DATGDI010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 20874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20874
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241963"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VKO45_03695"
     CDS             complement(<1..828)
                     /locus_tag="VKO45_03695"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HMA05009.1"
                     /translation="MGDGVRKIAENEWEVPVGFTPGMRVPGRFFLSDGLRKSLEPGAI
                     QQLANVATMPGIVKYALAMPDIHWGYGFPIGGVAAFGISDGVISPGGVGFDINCGVRL
                     MATPLTVQDLKNRRELVERLYEAVPTGVGAKATLRLSNRELEEMMVTGSRWAVEHGYG
                     VPRDIARCEEQGSMKGADPGSVSPKARQRGFPQAGTLGSGNHFLEIQEVREVYDPKAA
                     SLFGISRGQVTVMIHCGSRGLGHQVCTDHLRELETATKKYGIRLPDRQLACAPITSPE
                     "
     gene            complement(837..1244)
                     /locus_tag="VKO45_03700"
     CDS             complement(837..1244)
                     /locus_tag="VKO45_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HMA05010.1"
                     /translation="MSYEVLEHTADLRFRVRGRTLGEAFAEAARAVCETMAPNCTPGE
                     VVRRVEIEAPDRETLLADFLSEILYLSDAESLAFCSFGVEIRGNRLSAVLGGEPFRRE
                     KHGGGEEIKGISYSGLSLYQEGESYVLEFIADV"
     gene            complement(1250..1516)
                     /locus_tag="VKO45_03705"
     CDS             complement(1250..1516)
                     /locus_tag="VKO45_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05011.1"
                     /translation="MDRTSPSPADDGIRVTEEVRRYLEARRHDMRLCTSCGGPILLPT
                     SIHPPKPTDLRIRVGGWTVYVSRYQARYHDTIHAGMIPRFMDRF"
     gene            complement(1790..2101)
                     /locus_tag="VKO45_03710"
     CDS             complement(1790..2101)
                     /locus_tag="VKO45_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2551 domain-containing protein"
                     /protein_id="HMA05012.1"
                     /translation="MGSSDGFRREIRRRLKGYLLRDRTGIRREVLALFLRLRSLTIPR
                     IYGVLTRRFSVSYHALASMVGIIAARIGILHVKKQQDSPYAVYELREEYVDVVTQLLV
                     A"
     gene            2436..3290
                     /locus_tag="VKO45_03715"
     CDS             2436..3290
                     /locus_tag="VKO45_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HMA05013.1"
                     /translation="MDWFSYDINRYTNLKMVAIPATIFLISLAIVGMTYLSTGLPVQP
                     GIDFRGGTAVTLSTNDTQAAIEAYFAGFPLEPVTHDISSHVYLLTFTAMSADKNQQLI
                     DRINARYPDASVDYIDSTFGATLQGQALIALLFSFIGMAIVVFIAFRTFIPSIAVVSC
                     AFIDMVGTAAGMNLFGIQLTLPTTAALLMLIGYSVDSDILLTMRVIKRQGKLEDKLAG
                     AFRTGIIMTSTTLAAVLSLWVVSSLGQIEIIRDMAAVLLIGLVLDIINTWITNATLLK
                     WYAEGKVT"
     gene            3292..4809
                     /locus_tag="VKO45_03720"
     CDS             3292..4809
                     /locus_tag="VKO45_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071806 - protein transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HMA05014.1"
                     /translation="MAKPRKKKEGEAAAKQQPQAPARKEGLRELVRDWRVLLLIVLIV
                     LSVGAIYPHFENGQFTTALQFGLDLQGGSWLQMSFNSEVVGFTSQRTADDLASALNKR
                     LDAEVSVVGPGQLEIRKSIGQAELEQAFTAENATLTTYTQGVSASTGNDIKRILEQKI
                     NTLGTKDARVNLLTPVGSNVVKYVRVELAGVDINTARNIVGKQGLFEIRIKTTGNETE
                     HVLYGNAITSVGNPQQDPTRMTWGVSFTLSEQGAAAFRAAANEYGAVTAPEAHPVSMV
                     LDNVTVFSAPLDVSLAKELLTTDVSRLQATTGGGTAGMTQAQNLEIHLRAGALPVDVQ
                     VANFGQVSAPLGEYFKLMSFVAAILALLTVGLVIYLRYREPSIVLPMVGTNFAEIIIL
                     LGIARFVQQLDLASIAGLIAVVGTGIDQLVVITDEVLHEGKVPSPALYLKRLTRALAI
                     ILVAAGTVVFAMLPLALMDLSTLKGFAFITILGVLIGVAVTRPAYGKIIMSVLSR"
     gene            complement(4877..5674)
                     /locus_tag="VKO45_03725"
     CDS             complement(4877..5674)
                     /locus_tag="VKO45_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05015.1"
                     /translation="MPPRGERLWGDPDNIPAMKEETRWFILAGAGLVALSALLYTVEY
                     LVFRDPNHLLLYFVGDLAFVPLEVLIVTLVIDRLLASRERESRREKMNMLFGAFFTRL
                     GTPLLARFTRADPGIDGLGPHLKVGTGWTPARFREVKNRLETYRCEISPGSVDLEALR
                     SFLAGHEDFLLRLIENPAIFEHEGFTDLLLALDHLYKELKAREDLAILPPTDRQHLSG
                     DILRVYSLLIPEWLSYMEYLKDRYPYLFSLAMRTNPFDPSASVVLKE"
     gene            5764..6027
                     /locus_tag="VKO45_03730"
     CDS             5764..6027
                     /locus_tag="VKO45_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMA05016.1"
                     /translation="MIDFYADWCGPCRLQTPIVEEFARRMGDQVEVKKINVDEHMDMA
                     NKYRIYVVPTLIIEKDAKEIRRVEGLTDASSLEAMVKPLVEKP"
     gene            6024..6671
                     /gene="npdG"
                     /locus_tag="VKO45_03735"
     CDS             6024..6671
                     /gene="npdG"
                     /locus_tag="VKO45_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HMA05017.1"
                     /translation="MKVGIVGGTGDIGEGMALRISQVHEVILGSREVERACATSNACH
                     LVLDEKGLPCTCSGVSNQEAVDRAEVVILAIPFQHLAATLSSLSGFGGKVVVSPVNPI
                     GRKEYFFFAPPPEGSAAQLVRSLLPAARVCTAFNNIAAGSWKAFDAPFAHSVAVCGDD
                     PAAKEVVSGLVASIPHLAPFDAGPLAASPMIEAITPLLLNIAKYQKMRDVGIRFS"
     gene            6649..7212
                     /locus_tag="VKO45_03740"
     CDS             6649..7212
                     /locus_tag="VKO45_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HMA05018.1"
                     /translation="MSGSGSASAEIARIGRRALAEGLVSGNFGNASLRTAGGFLVKRT
                     GAFLDEPGPLVPVPLDGEIPAGASRESLVHRTVYNLTPHRAILHIHPPYAVTLSFDRD
                     EIIPIDPEGMLLCPGIPVVTGDCGSQALGDRVAHALMHGKIVIARGHGTFAAGKDLEE
                     AYLVTAAAEHACRVLWLRDVLTGRGRK"
     gene            complement(7236..8159)
                     /gene="aglJ"
                     /locus_tag="VKO45_03745"
     CDS             complement(7236..8159)
                     /gene="aglJ"
                     /locus_tag="VKO45_03745"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer glycoprotein N-glycosyltransferase AglJ"
                     /protein_id="HMA05019.1"
                     /translation="MKVNPDEVCILIPTLNEAPTIGNVVKEFQQLGYRHILVVDGHST
                     DKTVEIARGLGVTVKTQAGKGKGEAIIEALEQVREPYIVLVDGDGTYTAKDAERLLRP
                     LYLGFDHAIGDRLNTADKGAFNILNHTGNVLINFLFKFAHGTDLHDILSGYRAFTLDS
                     IKGMRLKEKGFGIETEMTIEAVRNQQRIMVVPVHYRKRVGTRTKLDPFHDGVKIVTTI
                     YRLAKVNNPIFYFGLMGILLSLAGMGVGVYVVLEWFKGINHFPMTVLTVLLIMVGFEI
                     FMFGVISDILLAFHREVIHEIHLLQQPRKER"
     gene            8198..8782
                     /locus_tag="VKO45_03750"
     CDS             8198..8782
                     /locus_tag="VKO45_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HMA05020.1"
                     /translation="MHLAKRGIRALGIAESFSGRSSSTMAGVVMRKDLRIDGVALATL
                     TVGGMDATDAVLGIIASLARRDINVALVSGSVVAWYNILDPEAIQEKTGLPLIVVTYE
                     DSEGLEEDITHHFPGDEDRLKAYRRLGAREPVDLGGGSRVFIRPFGISSDDASRILRD
                     FTRDGKVPEPVRVARLVARAAMRWMGGGGAGERA"
     gene            complement(8779..9294)
                     /locus_tag="VKO45_03755"
     CDS             complement(8779..9294)
                     /locus_tag="VKO45_03755"
                     /inference="COORDINATES: protein motif:HMM:NF019407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HMA05021.1"
                     /translation="MDSRRSFPAVSPVIGAMLLVGMTVVLSALVYLLVLSAPPPVIDP
                     SRFQFIHITDIRHPGDPVATPCDDSCILLIHEGNAPLENDRLSAIILGNDEMVRANIT
                     TLNGKKYPATKHFGVKNLGGAGSRGSTWDPGEEIYIDLKEDLVKEGDLVTVRIVDKDS
                     NLVISEDTARA"
     gene            complement(9402..9746)
                     /locus_tag="VKO45_03760"
     CDS             complement(9402..9746)
                     /locus_tag="VKO45_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HMA05022.1"
                     /translation="MADLAEQALRIIQSSRDGVLQSELWKLLKVDSRKCSRIVKKLLD
                     SGQIERIEFKGDGIKTYTLRAMKKTVRPSLILAGGELVPCISCDRECVVTACNLLLDW
                     MYQLAIEEHTEG"
     gene            complement(9752..9988)
                     /locus_tag="VKO45_03765"
     CDS             complement(9752..9988)
                     /locus_tag="VKO45_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain-containing protein"
                     /protein_id="HMA05023.1"
                     /translation="MPTSMVLPIKKVFALVDSRITVEIKDEDRKFQGRLISVDEYLNI
                     HLDETIELIGNERGRSLGTVVIRGNNILTISPVV"
     gene            10237..11658
                     /locus_tag="VKO45_03770"
     CDS             10237..11658
                     /locus_tag="VKO45_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HMA05024.1"
                     /translation="MILGYGSVAKCTLPILLSHIQIPLDLITILDFEDKEEALAPWTA
                     RGIRYFRRRITPENLGSVLSEYLSPGGLLIDLAWNIDCCEIVSWCHDHDVLYVNTSVE
                     VWDSYGERFTADPYEKSLHRRQMKLRELAGDWEDAPTFIVDHGANPGLISHFAKQGAV
                     DIARRMIADGLARDPDLLERLIREGDFARLAREIGVRVIHCSERDTQISCRPKEVGEF
                     VGTWSIEGLREEGTAPAEMGWGTHEKELPPLAFLPPGEPRNQVMLAQMGINTWVRSWV
                     PGGEIVGMVIRHGEAFGISDRWTVWEKGRAVYRPTVHYAYVPCDATIASLQELRGRNY
                     ELQPRQRIMNDREIVSGADTLGALLMGHPYRSWWTGSILSIEEARRLAPGENATTIQV
                     ALGVVSAVMWMIENPRKGFCLPDDLPHEFVLSVAKPYLGEFSSGPSDWTPLSERTVYF
                     RENPASVADREDPWQFKNFLFVH"
     gene            11672..12883
                     /locus_tag="VKO45_03775"
     CDS             11672..12883
                     /locus_tag="VKO45_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HMA05025.1"
                     /translation="MATMEEARKGAKKEGQPDGVPHLDQAKKEKLQDLAREHGTPIFV
                     IDHEKIRENYREFRELLPRVQVYYAVKANSNPEIVKTLFSLGASFDVASMPEFMVVHD
                     NIRNMPDKERQDWIWDKIIYANTIKPIETLEALDQYKPLVTFDNIEELRKIRKHAPNA
                     GLVLRLRVPNTGSMVELSSKFGAHPGEAVDLIVEAFKTGLVVEGLSFHVGSQCTNFEN
                     YVQALEISANIIREAEERAGNRIKILDIGGGFPVKYNPEVRSLKVLARKLNAEIRRLF
                     TPDTEILAEPGRFMVADACSLVAKVIGKAVRDGKKCYYINDGIYHTYSGQIFDHCTYP
                     VLAFREGEREISSVFGPTCDAFDTVTLGAELPDLEIGDLVYSENIGAYSIASSTYFNG
                     FPPAKVVHINR"
     gene            complement(12917..13558)
                     /locus_tag="VKO45_03780"
     CDS             complement(12917..13558)
                     /locus_tag="VKO45_03780"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HMA05026.1"
                     /translation="MNPMEARKNPLQRYAATCGIVGPILYALVVFALGILTPGYSQAT
                     QLMSELGENGAPYALAMNLGGFLLLGVLLLIFSFGLHAALRPSRASRAGALLVVFAGL
                     TYIGEALFSCDRGCVPVTFGGSAHLLIGEIAVFVAVLASYTLALAMRPDGRWNGYWQY
                     SAATGILVILLIPLFPVFVGVTGAVQRLVVGVILLWLFVIALRAYRILGGNGT"
     gene            complement(13636..14403)
                     /locus_tag="VKO45_03785"
     CDS             complement(13636..14403)
                     /locus_tag="VKO45_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05027.1"
                     /translation="MEDPPVNLHVFAVLFAIAIIHPIFTLTRIFAQVLAQRVNPPGGF
                     PVNYIGLDFVVLTYLVLVAILGGLIYAALYLGKKVGLGAPLLEGWTKGEPVRDRALSA
                     LKIALAVGLGVAMAKYLLDLLVFSPFVPATLSQWGQAPLPFLLPIPFQQGIGDEIVSR
                     LFWMTVIVWIIWKIRGSGNEPLGGPTYWACILMAGLISIPGLMLSGTPGLVILQYAVL
                     ILAGAIPFGWLYWKKGIESALVAHFTSSVILVLLSLF"
     gene            complement(14495..15592)
                     /gene="fbp"
                     /locus_tag="VKO45_03790"
     CDS             complement(14495..15592)
                     /gene="fbp"
                     /locus_tag="VKO45_03790"
                     /EC_number="3.1.3.11"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase/phosphatase"
                     /protein_id="HMA05028.1"
                     /translation="MVKTSVSMIKADIGSYPGHSRPHPLLLEKAQKMLKEQEGKLLTD
                     SFVTHCGDDLELIMTHTKGVDNAEIHKLAWNVFLECAKIAKSMKLYGAGQDLLSDAFS
                     GNVKGMGPGVAEMEFEERGSDPLLCFMADKTEPGAWNFYLYKVFADPFNTSGLVIDPG
                     MHEGFIFEVHDVIEKRRIMFRTPEESYSLLAYIGAPSRYVVKHVFRKDGVIAASTSTQ
                     RLSLIAGKYVGKDDPVMMIRAQSGLPAVGEVIEPFATPIIVAGWMRGSHHGPFMPVGL
                     CDANCTRFDGPPRVICLGFQVANGKLIGPVDMFDDVAFDRVRSRCNELADILRAHGPF
                     EPHRLSLSEMEYTTLPLVEKALRNRWEKIPE"
     gene            complement(15973..16410)
                     /locus_tag="VKO45_03795"
     CDS             complement(15973..16410)
                     /locus_tag="VKO45_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMA05029.1"
                     /translation="MEDTPSDAFRRLQEILEQMIRESMEQGNFMVRPMGFTIVIRGTG
                     PFPLGQGIQAPESGGTLEPRIEVHEADGEILVLAELPGVSRDQVRFDLEEGALRIAAS
                     DGARQFVGRAELPPVDPDSLSASCRNGVLEVRFRRIPEPAGYS"
     gene            complement(16416..19127)
                     /locus_tag="VKO45_03800"
     CDS             complement(16416..19127)
                     /locus_tag="VKO45_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HMA05030.1"
                     /translation="MTSKDSAEVTVKEAYLGDPGRGIARISLDIMKSLNLVSGDVIEI
                     EGRKKATAVVWPGYPEDTRKAVIRIDGTIRANAGTGIDEKVSIRKIEVVYAKKVVVQP
                     TQPIRLVGGEQYLSRLLRGRSVQEGQAVRVDVLGNPLTFVIAKVIPKGVGVVTDDTEI
                     ELKEEAYEPGKEKKGKKEVSDVHYEDIGGLGRELQLVREMIELPLRHPELFERIGIEP
                     PKGVLLYGPPGTGKTLIAKAVANEVDAHFITLSGPEIMSKFYGESEKHLREVFEEASE
                     NAPAIIFIDELDSIAPKRAEVTGEVERRVVAQLLALMDGLKGRGQVVVIAATNLPDSI
                     DPALRRGGRFDREIEIGIPDKKGRMEIFQIHTRGVPLALEKVELTDEQKAKVEEDFNA
                     KKLDLDYYREKLITEKKREKFDRQAKPGVPFDPLKVSLTGEEMKAVDADYTRRFEEEK
                     KKIEEPYRKEKFLREYADTTHGFVGADIALLVKEAAMFALRCVIPKIKIDEAIPAEIL
                     DQLRVRKADFDEARKHVEPSAMREVLVEIPDVEWTDVGGLEEVKQELREAVEWPLKFG
                     EVYTALQTKPPKGILLFGPPGTGKTLLAKAVANESECNFISVKGPELLSKWVGESEKG
                     VREIFRKARQASPSIIFFDEVDALVPKRGMYMGSSHVTESVVSQILTELDGLEELKNV
                     VVLGATNRPDMIDPALLRPGRLDRIIYVPPPDAASRLKIFEVYLGDAKEKGVLSGEIS
                     LDDLVSRTDGYVGADIEALVREAKLAAMREIVGGFTGMSEAERKETVKKVQITPAHFE
                     TAFTRVKGSLDADTLEENERRSWEMIFNAEERKTLDGALSVLKRAELASGGSEPPEEL
                     AKGMAALKAGIFARRKDWKELKKARERLEKLLEKKQKLPEKVPLTAR"
     gene            complement(19296..19496)
                     /locus_tag="VKO45_03805"
     CDS             complement(19296..19496)
                     /locus_tag="VKO45_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05031.1"
                     /translation="MQISPGMAKLTNGQLQKVQKLEGELDVILIAHEKMPVLSDLSRE
                     ELKALREMEKRMSLTLVAYRAE"
     gene            19545..20108
                     /locus_tag="VKO45_03810"
     CDS             19545..20108
                     /locus_tag="VKO45_03810"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HMA05032.1"
                     /translation="MEEVIPCGILIGPRLPVLMMPPTPRLTGLPPVVGPDPVVLILGS
                     FPSVISLERGEYYANPRNRFWPLMEALLGIQATLPYHVRTVRLKEKRIALWDVTKECE
                     REGSDDAAMKNEVLNDIPGFLRDHPTISVIVLNGGGAGRIFHKSFADGIPGILILTLP
                     STSPANARVGLPQLADTWSALREFSGK"
     gene            complement(20136..>20874)
                     /locus_tag="VKO45_03815"
     CDS             complement(20136..>20874)
                     /locus_tag="VKO45_03815"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HMA05033.1"
                     /translation="CGLVSRGGAFLTAWMLHNKKENPLYSEFDYLLEILKEHEVTLSM
                     GNGMRAGAVHDATDRAGIQELIINAELADKATAAGVQTIVEGPGHIPLDEIEANVIIQ
                     KRLTNRKPFYMLGPLVTDIAPGYDDRVAAIGAAISSAHGADFICYVTPAEHLALPTPE
                     EVYEGVISTRIAAHVGDMVKLKKRDGDLEMGKARRSLDWGKQFRLAINPEKAKRIRAE
                     RMPGDSDVCTMCGDYCAMKIVNQYFAF"
BASE COUNT         3744 a         6757 c         6622 g         3751 t
ORIGIN      
        1 ctcgggggag gtaatggggg cgcaggcgag ctgccggtcg gggagccgga tgccgtactt
       61 cttcgtcgcg gtctcgagtt ccctgagatg gtcggtgcag acctggtggc cgaggccccg
      121 ggagccgcag tggatcatca cggtgacctg tccccgggag atgccgaaga gggaggcggc
      181 cttgggatcg tacacctccc tcacctcctg gatctccagg aagtggttcc ccgaccccag
      241 ggtccccgcc tgggggaagc cccgctgccg ggccttcggg cttaccgagc cggggtccgc
      301 ccccttcatc gagccctgct cctcgcatcg cgcgatgtcc cgggggaccc cgtacccgtg
      361 ctccactgcc caccgggagc cggtgaccat catctcctcc agctcccggt tcgagagccg
      421 gagggtggcc ttcgccccga cgccggtcgg aaccgcctcg taaagccgct caacgagttc
      481 ccgccggttc ttgaggtcct ggaccgtgag cggggtcgcc atgagccgca ccccgcagtt
      541 gatgtcaaag cccacccccc cgggggagat gacaccgtcg gagatcccga aggccgcgac
      601 ccccccgatg gggaagccgt agccccagtg gatgtcgggc atggcgaggg cgtacttcac
      661 gatgcccggc atcgttgcga cgttcgcgag ctgctggatg gcccccggct ccaggctctt
      721 cctcagcccg tcggaaagga agaaccggcc cgggacccgc atcccgggcg tgaacccgac
      781 cggcacctcc cactcgtttt ccgcgatctt cctgacgcca tcacccatac ggaccctcac
      841 acgtcagcga tgaactccag tacataactc tccccctcct gataaagaga gaggcccgag
      901 tacgagatcc ccttgatctc ctcgcccccg ccgtgcttct cccggcggaa gggctcgcct
      961 cccaggaccg ccgagagccg gttcccccgg atctccaccc cgaaggagca gaaggcgagg
     1021 gactcggcgt cggagagata gaggatctcg gagaggaaat cggcgaggag ggtctcgcga
     1081 tccggggcct cgatctcgac ccgtcggacc acctcccccg gcgtgcagtt cggagccatg
     1141 gtctcgcaga ccgcccgggc agcctcggcg aaggcctccc cgagggtgcg gcccctcacc
     1201 cggaacctca ggtctgctgt gtgctcgagg acctcgtagg acatatccgc tagaacctgt
     1261 ccatgaaccg ggggatcatc ccggcatgga tggtgtcatg gtaccgggcc tggtaccggg
     1321 agacgtacac ggtccagcca cccacccgga tccggaggtc ggtcggcttc ggcgggtgga
     1381 tggaggtggg gaggaggatc gggccgccgc aggaggtgca caggcgcatg tcatgtctcc
     1441 gggcctccag gtatctccgc acctcctcgg ttacccggat cccgtcgtca gccggggaag
     1501 gagaggtgcg gtccatgggg agcgttgcac cggggggttg aaaaaggtgg cggggatccc
     1561 gggtcccgca ggaagccccc ggctccgaag gaggcggggc tgtcccgggg gggttggggc
     1621 ccctcgggac cgagccggga gcgtccaggg gggaggagga gacacgaagt ggctcctcct
     1681 gcccaggggg gtaggggccc ctctgggcgg aagggagccc tctggacctg gacgggtccg
     1741 aaggagccgg agttgtccag gggggaaggg ggccctctcg acaggtcccc taggcgacga
     1801 ggagctgggt caccacgtcc acgtactcct ccctgagttc gtagacggcg tacgggctgt
     1861 cctgctgctt cttcacgtgg aggatcccga tccgggccgc gatgatcccc accatggagg
     1921 caagcgcatg gtagctcacc gagaaccgcc gggtgaggac cccgtagatc cggggtatgg
     1981 tgagagagcg gagccggagg aagagggcga gcacctccct ccggatcccg gtcctgtccc
     2041 ggaggaggta tcccttcagg cgcctcctga tctccctccg gaaaccatcg ctcgatccca
     2101 tacgggcacc atacagcagg gccgttctta agggttgtca tttgcactcg ttttgaggag
     2161 gaagggatgc ggggagcatc cctgacgacg agaaatgggt gcgggagaaa aaggtgccag
     2221 agcacctttt tcgaccagag gaggaacgac gagaaacggg tgcgggagaa aaggtgcgaa
     2281 gcgccttttt gacctgatag gaggaacgac gagagatggg tgccgagcag ccctttaagg
     2341 aggggatgcg ataaaggatg cgatagttcc atccccgcct tcctcctccg ggacccccaa
     2401 atcctttaat gcatccggcg gtcaccttgt acggtatgga ctggttctcc tacgacatca
     2461 accggtacac gaacctgaag atggttgcca tccccgccac catcttcctc atctccctcg
     2521 ccattgtcgg gatgacctac ctctccacgg ggcttcccgt gcagcccggc atcgacttcc
     2581 gggggggcac cgcggtcacc ctctcgacga acgacaccca ggcggccatc gaggcgtact
     2641 tcgcggggtt ccccctcgag ccggtcaccc atgacatctc ctcccacgtc tatctcctca
     2701 ccttcacggc catgtcggcg gacaagaacc agcagctcat cgacaggatc aacgcccggt
     2761 acccggacgc gagcgtggac tatatcgact cgaccttcgg ggccaccctc cagggccagg
     2821 ccctcatcgc cctcctcttc tccttcatcg ggatggccat cgtcgtcttc atcgcctttc
     2881 gcacctttat cccctccatc gcggttgtct cctgcgcctt catcgacatg gtggggaccg
     2941 cggccgggat gaacctcttc ggcatccagc tcactctccc caccaccgcg gccctcctca
     3001 tgctcatcgg ctactcggtg gacagcgaca tcctcctcac catgcgggtc atcaagcgcc
     3061 aggggaagct cgaggacaag ctcgccggcg ccttccgcac ggggatcatc atgacatcca
     3121 ccaccctcgc cgcggttctc tccctctggg tcgtgtcgag cctcgggcag atcgagatca
     3181 tccgggacat ggccgcggtg cttctcatcg gcctcgtcct tgatatcatc aatacctgga
     3241 tcacgaatgc caccctcctc aagtggtatg ccgaggggaa ggtgacctga catggcgaag
     3301 ccgaggaaaa aaaaggaggg cgaggcggcc gcgaagcagc agccccaggc gccggcgcga
     3361 aaggagggcc tgcgcgagct ggtgcgggac tggagggtcc tcctcctcat cgtcctcatc
     3421 gtcctctccg tcggggccat ctacccgcac ttcgagaacg gccagttcac gaccgccctc
     3481 cagttcggcc tcgacctcca gggagggtcc tggctccaga tgtcctttaa ctcggaggtc
     3541 gtggggttca cctcccagag aacggcggat gaccttgcct ccgccctgaa caaacgcctc
     3601 gatgcagagg tctcggtggt gggacccggc cagctggaga tccggaagtc catcggccag
     3661 gccgagctcg agcaggcctt cacggccgag aacgccacac tgaccaccta cacgcagggg
     3721 gtatccgcct cgaccgggaa cgacatcaag cggatcctcg agcagaagat caacaccctg
     3781 ggaacgaagg acgcccgggt gaacctcctc acgccggtgg gctcgaacgt ggtcaagtac
     3841 gtccgggtgg agctcgcggg ggtggacatc aacacggccc gtaacatcgt cgggaagcag
     3901 ggcctcttcg agatccggat caagaccacg ggaaatgaga ccgagcatgt cctctacggg
     3961 aatgccatca cctcggtggg caacccccag caggacccga cccggatgac ctggggggtc
     4021 tccttcaccc tctcggagca gggggcggcc gccttccggg ccgcggcgaa cgagtacggg
     4081 gcggtgaccg cccccgaggc ccatcccgtc tccatggtcc tcgacaatgt cacggtcttc
     4141 tctgcgcccc tcgacgtctc cctggcaaag gagctcctga ccaccgatgt ctcccggctc
     4201 caggcaacca ccggcggggg taccgccggc atgacccagg cccagaacct ggagatccac
     4261 ctccgggccg gggccctccc cgtggacgtg caggtggcga acttcggcca ggtctcggcc
     4321 cccctcgggg agtacttcaa gctcatgtcc ttcgtcgcgg cgatcctcgc cctcctcacc
     4381 gtggggctcg tcatctacct gcggtaccgg gagccctcca tcgtcctccc catggtgggg
     4441 acgaacttcg ccgagatcat catcctcctc gggatcgccc ggttcgtcca gcagctggac
     4501 ctcgcctcca tcgcgggcct catcgcggtg gtggggaccg ggatcgacca gctggtggtc
     4561 atcaccgacg aggtcctgca cgagggaaag gtcccctccc ccgccctcta cttgaagagg
     4621 ctgacccggg ccctcgccat catcctcgtt gcggcgggca cggtggtctt cgccatgctc
     4681 cccctcgccc tcatggacct ctcgaccctg aagggctttg ccttcatcac catcctcggg
     4741 gtcctcatcg gggtcgcggt cacccggccg gcctacggga agatcatcat gtccgtgctc
     4801 tcgagatagg ccccttctcc tttttcccct aagacgagta cgggtccggt ctgccttccc
     4861 cccgggaaaa atccggctac tccttcaaga ccaccgaagc ggagggatcg aaggggttcg
     4921 tccgcatcgc gagggagaag aggtacgggt accggtcctt caggtattcc atgtagctca
     4981 gccactcggg gatgagcagg gaatacaccc gcaggatatc accggagagg tgctgccggt
     5041 cagtcggggg aaggattgcg aggtcctccc gggccttcag ctccttgtag agatgatcca
     5101 gggcgaggag gagatcggtg aagccctcgt gctcgaagat ggccgggttc tcgatgagcc
     5161 tgagaaggaa gtcttcgtgg ccggcaagga aggaccggag cgcctccagg tcgaccgatc
     5221 caggtgagat ctcacaccgg taggtctcca gccggttctt cacctcccgg aaccgggcgg
     5281 gcgtccagcc ggttccgact ttgagatgag gccccaggcc atcgatgccc gggtccgcgc
     5341 gggtgaaccg ggcgaggaga ggcgtcccga ggcgggtgaa aaatgcaccg aaaagcatgt
     5401 tcatcttctc cctccgggac tccctctccc gggacgcgag gagcctgtcg atgacgaggg
     5461 tgacgatgag cacctcgagg ggcacgaagg cgaggtcccc cacgaagtag aggaggaggt
     5521 ggttcgggtc ccggaagacc aggtactcga cggtatagag aagggcggag agggcgacga
     5581 ggcccgctcc cgcgaggatg aaccaccggg tctcttcctt catcgccggg atgttgtccg
     5641 ggtcccccca taatcgttcg ccccgcggcg gcatgcccct ttcccggatc gcggcctccg
     5701 tgtacgaatc tttattttcc accgggatcg agcttcttcc ggtgacccat gggaaaacct
     5761 gttttgatcg acttttacgc tgactggtgc gggccctgcc ggctccagac ccccatcgtc
     5821 gaggagtttg cgcggcggat gggcgaccag gtcgaggtga agaagatcaa cgtggacgag
     5881 cacatggaca tggccaacaa gtaccggatc tacgtcgtcc cgaccctgat catcgagaag
     5941 gacgcaaagg agatccggcg ggtcgagggg ctcacggacg cctcctccct cgaggccatg
     6001 gtgaagcccc tggtggagaa gccgtgaagg tcggtatcgt cggcgggacg ggcgatatcg
     6061 gggaggggat ggccctccgg atctcccagg tccacgaggt gatcctgggc tcccgggagg
     6121 tggagcgggc ctgcgcgacg agtaacgcct gccacctcgt cctcgatgaa aaggggcttc
     6181 cctgcacctg ctccggggtt tcgaaccagg aggcggtgga ccgggccgag gtggtgatcc
     6241 tggccatccc cttccagcac ctcgcggcga ccctctcctc cctctcgggc ttcgggggga
     6301 aggtggtcgt ctccccggtg aaccccatcg ggcggaagga gtacttcttc ttcgcgcctc
     6361 ctcccgaggg atcggcggcc cagctcgtcc ggagcctcct ccccgcggcg agggtctgca
     6421 ccgccttcaa caacatcgcg gccggatcct ggaaggcctt cgatgccccc ttcgcccatt
     6481 ctgtcgcggt ctgcggggac gacccggcgg caaaggaggt cgtgtccggc ctcgtcgcga
     6541 gtatccctca cctggccccc ttcgatgcgg ggcccctcgc cgcctcgccc atgatcgagg
     6601 ccatcacccc cctcctcctg aacatcgcga agtaccagaa gatgcgggat gtcgggatcc
     6661 ggttcagcta gcgcagaaat cgcccggatc gggagaaggg ccctcgccga aggcctggtc
     6721 tcggggaact tcgggaacgc aagcctgcga accgccggag ggttcctcgt caagcggacc
     6781 ggggccttcc tggacgaacc gggccccctg gtccctgtcc ccctcgatgg cgaaatcccg
     6841 gccggcgcct cccgggagtc cctcgtccac cggacggtat acaacctgac gccccaccgg
     6901 gccatcctcc acatccatcc accctacgcg gtcaccctct ccttcgacag ggatgagatc
     6961 atccccatcg accccgaggg aatgctcctc tgcccgggca tacccgttgt gaccggcgac
     7021 tgcgggtcac aggccctcgg tgaccgggtg gcccatgccc tgatgcacgg gaagatcgtc
     7081 atcgcgaggg ggcacggtac cttcgccgcg ggaaaagacc ttgaggaggc atacctggtc
     7141 accgccgccg ccgagcatgc ctgccgcgtg ctctggctta gagacgtcct cacgggacgg
     7201 ggcaggaaat aagactttta caactgggct tcccttcacc gctctttccg tggctgctgg
     7261 aggaggtgga tctcgtgaat gacctcccgg tgaaaggcga ggaggatgtc gctgatcacc
     7321 ccgaacatga agatctcgaa ccccaccatg atgaggagca cggtgaggac ggtcatgggg
     7381 aagtggttga tccccttgaa ccactcgaga accacgtaaa ccccgacccc catcccggcg
     7441 agggagagga ggatccccat gaggccgaag tagaagatgg ggttgttcac cttcgcgaga
     7501 cggtagatgg tggtgacgat cttcaccccg tcgtggaacg ggtcgagctt cgtccgggtc
     7561 cccacccgct tccggtagtg gacggggacc accatgatcc gctgctggtt ccggaccgcc
     7621 tcgatggtca tctcggtctc gatgccgaag cccttctcct tcaggcgcat gcccttgatg
     7681 gaatccaggg tgaaggcccg gtagccggag aggatgtcgt ggaggtcagt cccgtgggcg
     7741 aacttgaaga ggaagttgat gagcacgttc cccgtgtggt tcaggatgtt aaaggcaccc
     7801 ttgtccgcgg tgttcagccg gtccccgatg gcatggtcga acccgaggta caggggccgg
     7861 aggagcctct ccgcgtcctt cgccgtgtag gttccgtcgc catcgacgag gacgatgtac
     7921 ggctccctga cctgctcgag ggcctcgatg atggcctccc cctttccctt cccggcctgg
     7981 gttttcaccg tcaccccgag gcccctcgcg atctcgacgg tcttgtccgt ggagtggccg
     8041 tccacgacga ggatgtgccg gtacccgagc tgctggaact ccttcaccac attcccgatg
     8101 gtgggggcct cgttcagcgt cgggatgagg atgcagacct cgtcggggtt gaccttcatc
     8161 gatatactat ttaaaatcac ccgtcataag accttgcatg cacctcgcga aacggggaat
     8221 ccgggccctg ggaatcgccg aatccttctc cgggcggtcc tcctccacca tggccggggt
     8281 ggtgatgagg aaggacctcc ggatcgacgg ggtcgccctc gccaccctca cggtcggagg
     8341 aatggacgcc accgatgccg ttctcgggat catcgcctcc ctcgcccgcc gtgacatcaa
     8401 cgtcgccctg gtgagcggct cggtggtggc ctggtataat atcctggacc ccgaagcgat
     8461 ccaggagaag accggcctcc ccttgatcgt ggtgacctac gaggattcgg agggcctcga
     8521 ggaggatatc acccaccatt ttccgggcga cgaggaccgt ctcaaagcgt accggaggct
     8581 cggtgcccgg gagcccgttg acctcggagg tggatcccgg gtcttcatcc gccccttcgg
     8641 catatcatcg gacgatgcat cgaggatcct ccgggacttc acccgggacg ggaaggtccc
     8701 cgagccggtc cgggtcgccc ggctggtcgc ccgggccgcc atgaggtgga tggggggcgg
     8761 aggtgccggg gaacgggcct aggcccgggc cgtgtcctcg gagatgacca ggttggaatc
     8821 cttgtcgacg atccggaccg tgacgaggtc tccctccttc acgagatcct ccttgaggtc
     8881 gatatagatc tcctcgccgg ggtcccaggt cgacccccgg gacccggccc cgccaaggtt
     8941 tttcaccccg aagtgcttcg tggcgggata cttcttcccg ttcagcgtcg tgatgttcgc
     9001 cctcaccatc tcatcattcc cgaggatgat ggcggagagc cggtcgtttt ccaggggtgc
     9061 attgccctcg tgaatgagga ggatgcagct atcgtcacag ggcgtggcga cgggatcccc
     9121 cgggtgccgg atatcagtga tatggataaa ctggaaccgg gacggatcaa ttacgggcgg
     9181 cggggcggaa aggacgagga ggtagacgag ggccgagagg acgacggtca tccctacgag
     9241 gagcatcgct ccgatcaccg gtgaaacggc agggaaggac cggcgggaat ccatgaacgt
     9301 tccggaaata tgacccgacg gtgataaagg ttccgaaaga gggttgaaag tggaatccct
     9361 tacgtggggg agaaggagac aaggcggaac gagaaagggg gtcagccttc agtgtgctcc
     9421 tcgatggcga gctggtacat ccagtcgagg aggagattgc aggccgtgac cacgcactcc
     9481 cggtcgcagg agatacaggg cacgagttcc ccgccggcga ggatgaggga gggtcggacg
     9541 gtctttttca tggcccggag ggtgtaggtc ttgatcccgt cccccttgaa ctcgatccgc
     9601 tctatctggc cgctgtccag gagcttcttc acgatccggg agcacttgcg gctgtccacc
     9661 ttgaggagct tccagagctc gctctggagc acgccgtcgc ggctcgactg gatgatccgg
     9721 agtgcctgct ctgcgaggtc tgccataggt tttagacgac aggcgagata gtcaggatgt
     9781 tgttcccgcg gatgaccacg gtccccaggc tgcgccccct ctcgttcccg atgagttcga
     9841 tggtctcgtc caagtggatg ttcaggtact cgtccacgct gatgagccgg ccctggaact
     9901 tccggtcctc gtccttgatc tccacggtga tccgtgagtc caccagggcg aagaccttct
     9961 taatcgggag caccatgctc gttggcatct tcggtaatct ggttggtgcg gggttattaa
    10021 ggtttcttcc cgctcttccc gtgaaaaatg agacttttac cgccttcctt cccatgcgcg
    10081 ggaaccgggg tccgggtgcg atggggaggg atccggtttt taggaattgc agcctccccc
    10141 gccggcaggc tgctctttgg taagaatgaa ataatttccc ctcccatcag tgttcacagc
    10201 cggagggctt tcaaattgga gttcaggaac agggtcttga tcctcgggta cgggtcggtc
    10261 gccaagtgca cgctcccgat cctcctctcg cacatccaaa tccccctcga ccttatcacc
    10321 atcctcgact tcgaggacaa ggaggaggcc ctcgccccct ggacggccag ggggatccgg
    10381 tacttccggc gcaggatcac gccggaaaac ctgggatccg tcctctccga gtacctctcc
    10441 ccgggggggc tcctcatcga cctcgcctgg aacatcgact gctgcgagat cgtcagctgg
    10501 tgccatgacc atgacgtcct ctacgtgaac acctccgtcg aggtctggga ctcgtacggg
    10561 gagcgcttta cggcagaccc gtacgagaag tccctccacc ggcggcagat gaaactcagg
    10621 gagctcgcgg gggactggga ggatgccccg accttcatcg tcgatcatgg cgcgaacccc
    10681 ggcctcatct cccatttcgc gaagcagggg gcggtcgaca tcgcccgccg gatgatcgcc
    10741 gacgggctgg cccgggaccc ggatctcctg gagaggctca tccgggaggg tgatttcgcc
    10801 cgcctggccc gggagatcgg cgtccgggtc atccactgct cggagcggga cacccagatc
    10861 tcctgccggc cgaaggaggt cggggagttc gtcggcacct ggtccatcga gggtctccgg
    10921 gaggagggga ccgccccggc cgagatgggg tggggtaccc atgagaagga gcttcccccg
    10981 ctcgcctttc tcccgcccgg ggagccgagg aaccaggtca tgctcgccca gatggggatc
    11041 aacacctggg tgcggtcctg ggtcccgggc ggggagatcg tcgggatggt gatccggcac
    11101 ggggaggcct tcgggatctc tgaccggtgg accgtctggg agaagggaag ggcggtctat
    11161 cgccccacgg tgcactacgc gtacgtgccc tgcgacgcga ccatcgcctc cctccaggag
    11221 ctcaggggaa ggaactacga gctccagccg aggcagagga tcatgaacga ccgggagatc
    11281 gtctcggggg cggacaccct gggcgccctc ctcatggggc acccgtacag gtcctggtgg
    11341 acggggagca tcctctccat tgaggaagcg aggaggctcg ccccggggga gaacgcgacg
    11401 accatccagg tggccctcgg ggtggtgtcg gcggtcatgt ggatgataga gaacccgagg
    11461 aaggggttct gcctgccgga cgaccttccc cacgaattcg tcctctcggt ggcaaaaccc
    11521 tacctggggg aattttcctc cgggccctcg gactggaccc ccctctccga gaggacggtg
    11581 tacttcaggg aaaacccggc gagcgtagcc gaccgggagg atccctggca attcaaaaac
    11641 ttcttattcg tgcactgatg caggatacgt aatggcaacc atggaagagg cgaggaaggg
    11701 agcgaaaaag gagggacagc ccgacggagt gccccacctg gaccaggcga agaaggagaa
    11761 gttgcaggac ctggcacggg agcacgggac cccgatcttt gtcatcgacc acgagaagat
    11821 ccgggagaac taccgggagt tccgggagct ccttccccgc gtccaggtct actacgccgt
    11881 gaaggcgaac tcgaaccccg agatcgtgaa gaccctcttc tccctgggcg cgagcttcga
    11941 tgtcgcctcc atgccggaat tcatggtcgt ccacgataac atcaggaaca tgccggacaa
    12001 ggagaggcag gactggatct gggacaagat catctatgcc aacaccatca agcccatcga
    12061 gaccctggag gccctcgacc agtacaagcc cctcgtcacc ttcgacaaca tcgaggaatt
    12121 aagaaagatc cggaaacacg cacccaatgc cggcctcgtt ctcaggctgc gggtcccgaa
    12181 caccgggtcg atggtggagc tctcctccaa gttcggggcc caccccggcg aggcggtcga
    12241 cctgatcgtg gaggccttca agaccggcct cgtcgtggag ggcctctcct tccacgtcgg
    12301 cagccagtgc acgaacttcg agaactatgt gcaggccctc gagatctcgg caaacatcat
    12361 ccgggaggcc gaggagaggg cgggaaaccg gattaaaatt ctggacatcg gtggcggttt
    12421 tccggtcaag tacaaccccg aggtgaggtc cctcaaggtc ctcgcccgga agctgaacgc
    12481 cgagatccgg cggctcttca ccccggacac cgagatcctc gccgagcccg gccggttcat
    12541 ggtggccgac gcctgctccc tcgtggcaaa ggtcatcggg aaggcggtcc gggacgggaa
    12601 gaagtgctac tacatcaacg acgggatcta ccacacgtac tcggggcaga tcttcgacca
    12661 ctgcacctat cccgtcctcg ccttcaggga gggggagagg gagatctcct cggtcttcgg
    12721 cccgacctgc gacgccttcg acaccgtcac cctcggggcg gaactcccgg atctcgagat
    12781 cggggatctc gtgtactccg aaaacatcgg cgcctattcc atcgcctcct ccacctactt
    12841 caacggcttc ccgccggcga aggtggtgca catcaaccgg tgagtgtgct ccacctcgga
    12901 tcaccgggac aggcgctcag gtcccgtttc caccgagaat ccgataggcc ctcagcgcga
    12961 tgacaaacag ccagaggagg atcaccccca ccaccagccg ctgcacggcc cccgtcaccc
    13021 cgacaaaaac cgggaacagg ggaatgagga ggatgacgag gatcccggtg gcggctgagt
    13081 actgccagta tccattccac cggccatccg gtctcatggc gagggcgagg gtgtaagaag
    13141 caaggacggc gacaaaaacg gcgatctccc cgatgaggag atgggccgat cccccaaagg
    13201 ttacgggaac gcatcccctg tcgcaggaga aaagggcctc cccgatgtag gtgagcccgg
    13261 caaaaacaac aaggagtgcc cctgcccggc ttgcccgcga tggacggagg gcggcgtgca
    13321 ggccgaagga gaagatgagg aggagaactc cgagaaggag gaaccctccc aggttcatcg
    13381 cgagggcata gggggctccg ttctccccga gctcgctcat cagctgcgtg gcctgggagt
    13441 agcccggcgt gaggatcccc agcgcaaaga ccacgagagc atagaggatg gggccgacga
    13501 tgccacaggt ggcggcatac ctctgaaggg ggtttttcct ggcctccatg ggattcatcc
    13561 ctcaaccggt agaggttatg agggaaaaaa ggatctcgtc cacaccggaa aacaggccgc
    13621 gagggaggat ccctttcaga acagggacag cagcacaaga atgacgcttg aagtgaaatg
    13681 ggcgacgagg gcggactcga tccccttctt ccagtagagc cagccgaagg ggatggcccc
    13741 cgcgaggatc agaacggcat actgaagtat cacaaggcca ggtgtcccgg agagcatcag
    13801 gcccgggatc gagatgagcc cggccatgag gatgcacgcc cagtaagtcg ggcccccgag
    13861 cggttcattt cctgaccccc ggattttcca gatgatccat acgatgacag tcatccagaa
    13921 caggcggctg acgatttcat ccccgatccc ctgctggaag ggaatgggaa ggaggaacgg
    13981 aaggggggcc tgcccccact gtgacagagt ggccggcacg aagggtgaaa ataccagaag
    14041 gtccagcagg tacttcgcca tggcaacgcc gagaccgacg gcaagggcga tcttcagggc
    14101 agagagggcg cggtctctta ccggctctcc ctttgtccag ccttcaagga gcggggcccc
    14161 gaggccgacc ttcttcccca gatacagggc ggcatagatg agacccccga ggatcgcgac
    14221 caggacgagg tacgtgagga ccacgaaatc gaggccgatg taattgacgg gaaatccacc
    14281 agggggattc accctctgcg cgaggacctg agcgaagatg cgggtaaggg taaagatagg
    14341 gtgaatgatg gcgatggcaa agaggacggc aaacacatgc aggttgacgg ggggatcttc
    14401 catacgcgta tttagttcag atcgtctata aagaagagag gtgggggggg catggccggg
    14461 cctcccgcac ccgccacccg cggtgcctgc cgctttattc cggtatcttc tcccaccggt
    14521 tcctgagcgc cttctcaacc agcgggaggg tggtgtactc catctccgag agggagagcc
    14581 ggtggggctc gaaaggcccg tgagcccgga gaatgtcggc gagctcgttg caccgtgagc
    14641 ggacccggtc gaaggccacg tcatcgaaca tgtccacggg gccgatgagc ttcccgttcg
    14701 caacctggaa gcccaggcag atgacccggg gcgggccgtc gaaccgggtg cagttcgcgt
    14761 cacagagccc tacaggcatg aaggggccgt ggtgggagcc cctcatccag ccggcgacga
    14821 taatgggagt cgcgaagggc tcgatgacct ccccgacggc ggggaggccg ctctgggccc
    14881 ggatcatcat gaccgggtcg tccttgccca cgtacttgcc ggcgatgagg gagagccgct
    14941 gggtggaggt cgaggcggcg atgaccccgt ccttccggaa gacgtgcttc accacgtacc
    15001 gggagggcgc cccgatgtag gcgaggaggc tataggactc ctcgggggtc ctgaacatga
    15061 tgcgcctttt ctcgatgacg tcatggacct cgaagatgaa gccctcgtgc atccccgggt
    15121 cgatgacgag ccccgaggtg ttgaagggat cggcgaagac cttgtacagg tagaagttcc
    15181 aggcaccggg ctcggtcttg tcggccatga agcagaggag ggggtcggag ccccgctcct
    15241 cgaactccat ctcggcgacc cccggaccca tgcccttcac gttccccgag aaggcgtcgg
    15301 agagaaggtc ctggccggcc ccgtagagct tcatgctctt cgcgatcttc gcgcactcca
    15361 gaaagacgtt ccaggcgagt ttgtggatct cggcattgtc cacgcccttc gtgtgcgtca
    15421 tgatgagctc gaggtcgtca ccgcagtggg tgacgaaaga atcggtaagg agcttcccct
    15481 cctgctcctt gagcatcttc tgggccttct ccaaaaggag cgggtgggga cgggagtgtc
    15541 ccggataact cccgatgtcc gccttgatca tcgagacgct ggtcttgacc atacgatgca
    15601 cctacctact aacagatccc gagactatat ctttcctttg attttgctga cggcgagcga
    15661 ggcgagccga gaaccaccga aggtggtgac cggcgagcga agcgagccga gaaccaccga
    15721 aggtggtgac cggcgagcgg atcgcgacgg cggcgaacgc agttcgacgc acggcgagcg
    15781 aggcgagccg agaaccacca aaggtggtga ccggcgcggg tcgcgacggc ggcgaacgga
    15841 gtgcgccggg agacgcatat cgatgtcgcg acggcgagcg cggcaagtcc ggggtgcaca
    15901 cggggtgcca cacgacgcgg aacgcggccg cattgtccgt caggaattcg cagagaaaaa
    15961 tgatggtgtg tttcagctgt atcctgccgg ttcgggaatc ctccggaacc gcacctcgag
    16021 gaccccgttc ctgcagctcg cgctgaggga gtcgggatcg accgggggga gttctgccct
    16081 gcccacgaac tgccgtgccc cgtctgaagc ggcgatccgc agggctccct cctcaagatc
    16141 gaaccgtacc tggtccctgg ataccccggg gagctccgcc aggacgagga tctccccgtc
    16201 cgcctcgtgg acctcgatgc gcggctcgag ggtcccgccg ctctccggcg cctggattcc
    16261 ctgccccagg gggaaggggc cggtcccccg gataacgatg gtgaaaccca tgggcctcac
    16321 catgaagttt ccctgttcca tactctcgcg tatcatctgt tccaggatct cctggagcct
    16381 cctgaatgca tcgctgggtg tgtcttccat ggaacctacc ttgccgtgag aggcaccttc
    16441 tccgggagct tctgcttctt ctcgagcagc ttctccagcc tctccctcgc tttcttcaac
    16501 tccttccagt ccttgcgcct cgcaaagatc ccggccttta aggccgccat tccctttgcg
    16561 agctcctcgg gaggttcgga cccgcccgag gcgagttccg cccgcttgag gacagagagg
    16621 gcaccgtcca gggtcttcct ttcctcggca ttgaagatca tctcccagga ccgccgctcg
    16681 ttctcctcca gggtatcggc gtcgagggat cccttcaccc gggtaaaagc ggtctcgaag
    16741 tgggcagggg tgatctgcac cttcttcacc gtctccttcc gctccgcctc gctcattccc
    16801 gtgaaccccc cgacgatctc ccgcatggcg gcgagcttcg cctcccggac gagggcctcg
    16861 atgtccgccc cgacgtaccc gtcagtccgg ctcaccaggt cgtccaggct gatctctccc
    16921 gagaggaccc ccttctcctt tgcatctccg agatacacct cgaagatctt cagccggctc
    16981 gccgcgtccg gcggcgggac atagatgatc cggtccaggc ggcccggccg gaggagggcc
    17041 gggtcgatca tgtccgggcg attcgtcgcc ccgaggacca ccacgttctt cagctcctcg
    17101 aggccgtcga gctcggtgag gatctgggag accaccgact cggtgacatg ggaggagccc
    17161 atgtacatcc ccctcttcgg gacgagggcg tccacctcgt cgaagaagat gatggagggc
    17221 gaggcctgcc gggccttccg gaagatctcc cgaacaccct tctcggactc ccccacccac
    17281 ttcgagagga gctcggggcc cttcaccgag atgaagttgc actccgactc gttggccacg
    17341 gcctttgcaa ggagggtctt ccccgtcccc ggcgggccga agaggaggat ccccttcggg
    17401 ggcttcgtct ggagggcggt gtagacctcc ccgaacttga gcggccactc caccgcttcc
    17461 cggagctcct gcttgacctc ctcgagcccg cccacgtcgg tccactccac atccgggatc
    17521 tccacgagga cctcccgcat ggccgagggc tccacgtgct tcctcgcctc gtcgaagtcg
    17581 gccttccgga cacggagctg gtccaggatc tcggccggga tggcctcgtc gatcttgatc
    17641 ttcgggatga cgcaccggag ggcgaacatc gccgcctcct tcacgaggag ggcgatgtcg
    17701 gcccccacga agccgtgggt ggtgtcggcg tactcccgga ggaacttctc cttccggtac
    17761 ggctcctcga tcttcttctt ctcctcctcg aaccggcggg tgtagtcggc gtcgaccgcc
    17821 ttcatctcct cgccggtgag ggagaccttc aggggatcga agggcacgcc gggcttcgcc
    17881 tgacggtcga acttctcccg cttcttctcg gtaatgagtt tctcccgata gtagtccagg
    17941 tcgagcttct ttgcattgaa gtcctcctcg acttttgcct tctgctcgtc ggtgagctcc
    18001 accttttcaa gggcgagagg gaccccccgg gtgtggatct ggaagatctc catccggccc
    18061 ttcttgtccg ggatcccgat ctcgatctcc cggtcgaacc gcccgccccg gcggagggca
    18121 gggtcgatgg aatcggggag gttcgtcgcg gcgatgacca cgacctgccc ccggcccttg
    18181 agcccgtcca tgagggcgag gagctgggcc acgacccggc gctccacctc cccggtcacc
    18241 tcggcccgct tcggggcgat ggagtccagc tcgtcgatga agatgatggc cggggcgttc
    18301 tcgctcgcct cctcgaagac ctcccggagg tgcttctcgg actcgccgta gaacttggac
    18361 atgatctccg ggcccgagag ggtgatgaag tgggcgtcca cctcgttcgc cacggccttg
    18421 gcgatgaggg tcttcccggt acccggcgga ccgtagagga ggaccccctt cgggggctcg
    18481 atgccgatgc gctcgaagag ctcggggtgg cgcaggggga gctcgatcat ctcccggacg
    18541 agctggagct cccggccgag gcccccgatg tcctcgtagt ggacgtcgga aacctccttc
    18601 ttccccttct tctccttccc cggctcgtag gcctcctcct tgagctcgat ctcggtatcg
    18661 tcggtgacga cgccgacccc cttcgggatg actttcgcga tgacgaaggt gagcgggttc
    18721 ccgaggacgt ccacccggac cgcctgcccc tcctggaccg agcggccccg gagaagccgg
    18781 gagaggtact gctcaccacc gacgagccgg atgggctgcg tcggctggac caccaccttc
    18841 ttcgcgtaca cgacctcgat cttccggatg ctcaccttct cgtcgatacc ggtgccggcg
    18901 ttcgcccgga tggtcccgtc gatccggatc accgccttcc tcgtgtcctc cgggtagccc
    18961 ggccagacga cggccgtcgc cttcttcctc ccctcgatct cgatgacgtc ccccgagacc
    19021 aggttcaatg acttcatgat gtcaaggctg atgcgggcga tgccccggcc cgggtcgccg
    19081 aggtacgcct ccttcaccgt gacttccgca gaatcctttg atgtcaacgt gataaactcc
    19141 tgtgagtgat ttaccttaag ttagggagtc tcactatata atggttcctc aggccggcgg
    19201 accgttttct cgcctgaacc gcctctccgg gtcggacttg cccccgggtt tccggatctc
    19261 ctcccttcgc ccggaaaagg ggagtcttcc ccgccttatt ccgccctgta cgccaccagg
    19321 gtgagactca tccgcttctc catctcccgg agggccttca gctcctccct tgagagatcc
    19381 gagaggaccg gcatcttttc gtgggcgatg aggatcacat cgagctcgcc ctcgagcttc
    19441 tggacctttt ggagctggcc gttcgtgagt tttgccatac cagggctgat ctgcatccct
    19501 tcaccccgtg catccctccc cgtggatggg gataaaggtt tgggatggag gaggtcatcc
    19561 cctgcggcat ccttatcggt ccccgcctcc ctgttctcat gatgcccccc accccccgcc
    19621 tcaccggcct ccctcccgtc gtcggcccgg atcccgtagt cctcatcctc ggttccttcc
    19681 cctcggtgat ctccctcgaa aggggtgaat actatgctaa tccccggaac cggttctggc
    19741 ccctcatgga ggcgctcctg gggatccagg ccaccctgcc gtaccatgtg aggaccgtcc
    19801 ggctcaagga gaagcggatc gccctctggg acgtgacgaa ggagtgcgag cgggagggga
    19861 gcgatgacgc agccatgaaa aacgaggtcc tgaacgatat ccccggtttc ctccgggacc
    19921 acccgaccat ctccgtcatc gtcttgaacg gaggaggggc cggccgtatc ttccacaagt
    19981 cttttgctga cggaatcccg ggtatcctga tcctcaccct cccttccacg agcccggcga
    20041 atgcccgggt cggcctgccg cagctcgccg atacgtggag tgcattgagg gaattttcgg
    20101 gaaaataaaa gaagggagga tgcaggggat cgccgtcaga aggcgaagta ctggttcacg
    20161 atcttcatgg cacagtaatc gccgcacatg gtgcagacgt ccgagtcccc cggcatccgc
    20221 tcggcccgga tcctcttcgc cttctcgggg ttgatggcga gccggaactg cttcccccag
    20281 tcgagggacc ggcgggcctt ccccatctcg aggtcgccgt cccgcttctt cagcttcacc
    20341 atgtccccga cgtgggccgc gatccgggtg gagatgaccc cctcatagac ctcctcgggg
    20401 gtggggaggg ccaggtgctc ggccggggtc acgtagcaga tgaagtcggc accgtgggcc
    20461 gaggagatgg ccgccccgat ggccgcgacc cggtcgtcgt agccgggcgc gatgtcggtg
    20521 acaaggggcc cgagcatgta gaagggcttc cggttggtga gccgcttctg gatgatgacg
    20581 ttcgcctcga tctcgtccag gggaatgtgc cccggcccct cgacgatggt ctggacccct
    20641 gcggcggtgg ctttatcggc aagctcggcg ttgatgatga gctcctggat gcccgcacgg
    20701 tcggtggcat cgtgcactgc ccctgcccgc ataccgttcc ccatggagag ggtgacctcg
    20761 tgctccttca ggatctcgag gaggtagtcg aactccgagt agaggggatt ctccttcttg
    20821 ttgtggagca tccacgccgt gaggaaggcg ccgccccggg agacgagccc gcag
//
LOCUS       DATGDI010000101         3059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_416444, whole genome shotgun sequence.
ACCESSION   DATGDI010000101 DATGDI010000000
VERSION     DATGDI010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416444"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VKO45_03820"
     CDS             complement(<1..250)
                     /locus_tag="VKO45_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HMA05034.1"
                     /translation="MKRKRPSPAPLPMTLEEAGERGMDALDIVLVTGDAYVDHPAFGT
                     AVIGRVLEAAGYRVGIIAQPDWRSRADFQRLGRPRLFFG"
     gene            complement(292..804)
                     /locus_tag="VKO45_03825"
     CDS             complement(292..804)
                     /locus_tag="VKO45_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HMA05035.1"
                     /translation="MNGTPEIPEAEIAALHDRLDREAEEGGYHLNPDRAFVRGLVHGL
                     IINTRRYGYQSCPCRLATGKKKADLDIICPCDYRDPDLADYGACYCALYVSDEVLAGR
                     RKVASIPERRPPPDVRARVPPSAGAAPSFTLGYPVWRCRVCGYLCARDEPPDICPICK
                     AKKDRFERFF"
     gene            complement(801..1196)
                     /locus_tag="VKO45_03830"
     CDS             complement(801..1196)
                     /locus_tag="VKO45_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HMA05036.1"
                     /translation="MKQPGLAALILLSLALGALLVLPGCMATTPPAPATSYPSYVMHI
                     PGNNHGTVLLVGRTSCPHCHDARVFLANLSIDYYWIDTDTLDQANITQVLNSLKGVCS
                     QIESVPILVVNNQQCIIGFDETRYREALG"
     gene            complement(1193..1474)
                     /locus_tag="VKO45_03835"
     CDS             complement(1193..1474)
                     /locus_tag="VKO45_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HMA05037.1"
                     /translation="MEFVHVKGKRKQGEVLLYALSTCGWCAKTKELLTNLGIDFSYLY
                     VDLLPKSEMEKAFDEVKRFNPACSFPTVVIDGGKTIVGFREQELREAFE"
     gene            1661..2143
                     /locus_tag="VKO45_03840"
     CDS             1661..2143
                     /locus_tag="VKO45_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05038.1"
                     /translation="MKRIPRDDTLVSSEMLEQAVKEILARTTLDRTHDIPYLAGYSRD
                     GKRIYIDRHLPVSFQSGGKVILTDRFLILHEALEKAVIDQLGLVYQHAHQLALREEEA
                     AVRAFGVSWSEYSEFMDRYIKEAADERLTRIPPDLDIKPYRDEHDLRLLRNMQEAMRR
                     "
     gene            2319..2861
                     /locus_tag="VKO45_03845"
     CDS             2319..2861
                     /locus_tag="VKO45_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05039.1"
                     /translation="MAKGVPGKKGVSRGVDDVIAILDGYDTFHWSRRTLARAADFMIL
                     VAAITLAGVLWNRPYLSTGTPQGFFVVDLSLILFGLSACIFAVFRAVLFMIEDYMEKA
                     VNGVQGIRNGTFSSGRRNLRRAIDSVSSRLQDSIGLWWTARVIVIRSAFVIIPGVLFY
                     LLGIANLVMYLVGMRIIPGL"
BASE COUNT          575 a          915 c          991 g          578 t
ORIGIN      
        1 ccccgaagaa gaggcgggga cgcccgagcc tctggaaatc ggcccgggat ctccagtccg
       61 gctgggcgat gatccccacc cggtatcccg cggcctcgag gacccggccg atcaccgcgg
      121 tcccgaaggc cgggtggtcc acgtaggcgt cgccggtgac gaggacgatg tcaagggcgt
      181 ccatcccccg ctccccggcc tcctcgaggg tcatggggag gggggcaggg gaggggcgct
      241 ttcttttcat ggccgcggga tccggaaaag ggagggaggg gagaatgttc gtcagaagaa
      301 ccgctcgaac cggtccttct tcgccttgca gatcgggcag atgtcaggcg gctcgtcccg
      361 ggcacagagg tacccgcaga cccggcaccg ccagaccggg tacccgaggg tgaaggaggg
      421 ggctgcgccc gcagagggag ggacccgggc ccggacatcg ggcgggggcc ggcgctccgg
      481 tatggaggct accttccttc tccccgcgag gacctcgtcc gagacgtaga gggcacagta
      541 gcaggccccg tagtcggcga ggtcggggtc ccggtagtca cagggacaga tgatgtccag
      601 gtccgccttc ttcttccctg tggcgagccg gcaggggcag gactggtatc cgtaacgcct
      661 cgtgttgatg atgagcccat ggacgaggcc ccggacgaag gcccggtcag ggttgaggtg
      721 atagccgcct tcctcggcct cccggtcgag ccgatcgtgg agggcagcga tctcggcctc
      781 cggtatctcg ggagtcccgt tcatccaagg gcctccctgt agcgggtctc atcgaacccg
      841 atgatacact gctggttgtt caccacgagg atcgggaccg actcgatctg gctgcaaacc
      901 cctttgagcg agttcaggac ctgcgtgata ttggcctggt cgagggtatc agtgtcgatc
      961 cagtagtagt caatgctcag gtttgcgaga aaaaccctgg catcgtggca atggggacag
     1021 ctggtcctcc ccacgaggag gaccgtacca tgattgttcc ccgggatatg catcacgtac
     1081 gaggggtacg aggtcgccgg ggcggggggg gttgttgcca tgcagcccgg gaggacgagg
     1141 agggctccga gggcgaggct caggaggatc agggcggcga gtcccggctg cttcattcaa
     1201 aggcctcccg gagctcctgc tcccggaacc cgacgatggt ctttccgccg tcgatgacca
     1261 ccgtggggaa ggagcatgcg gggttgaaac gcttcacctc gtcgaaggcc ttctccatct
     1321 cggacttcgg gaggaggtcg acgtacaggt acgagaagtc gatccccagg ttcgtgagga
     1381 gctccttcgt ctttgcgcac cagccgcagg tggagagggc gtagagaagg acctcgccct
     1441 gcttcctctt tcccttcacg tgaacgaact ccatgaaagc accatgccca tccgggtctt
     1501 caagctatcg tctggcagcg atatcaggtt atctggacgg gtcgcctccg gcagtcgggg
     1561 gtcgcgggca gagaccgatc tgcatcggca gcggtcaggt ataataaccg atcaccgtta
     1621 tgaaaatagt ggggacattc gacgccggga ggccgatgcc atgaaaagaa ttcctcggga
     1681 tgatacgctc gtctcatcgg agatgctcga gcaggccgtg aaggagattc tcgcccgcac
     1741 gaccctggat cgcacgcacg atataccgta cctggccggc tacagcaggg acggaaaaag
     1801 gatctatatc gaccggcatc tgccggtgtc gtttcaaagc gggggaaagg tgatcctgac
     1861 cgatagattc ctgatccttc acgaggctct cgagaaggcg gtcatcgatc agctcggact
     1921 cgtctaccag cacgcccacc agctcgccct gcgcgaggag gaggcggccg tcagggcgtt
     1981 cggggtatcc tggagcgaat attcggaatt catggaccgg tacatcaagg aagcggccga
     2041 cgagaggctg acccgcattc ccccggatct ggatatcaag ccttaccgcg atgagcacga
     2101 tctccggctg ctcaggaaca tgcaggaagc aatgaggagg taggcccgcg gaccggggga
     2161 accgtgtggt atcggaaaac ggtggtcagg gaaaacagaa ttcctcatca tgatatatcc
     2221 atggtacggg cttttcccgg ggatctgtca gggatatttg ttaccttttc aggccagata
     2281 accttcttta tataccgggg ctattatcct gaggtcacat ggcgaagggc gttcccggaa
     2341 agaagggggt atcccgaggg gtggacgatg tcatcgccat cctggacggg tacgacacct
     2401 tccactggtc ccgccggacc ctggcgcggg cggcggactt catgatcctc gtcgccgcca
     2461 tcaccctcgc gggggtcctc tggaaccgcc cctacctctc cacgggaacc ccgcaggggt
     2521 ttttcgtcgt cgacctctcc ctcatcctct tcggcctctc cgcctgcatc ttcgcggtct
     2581 tccgggcggt tctcttcatg atcgaggact acatggaaaa ggcggtcaac ggggtgcagg
     2641 ggatccggaa cggcaccttc tcctcaggga gaaggaacct ccggcgggcc atcgactcgg
     2701 tctcctcccg gctccaggac tccatcggcc tctggtggac ggcccgggtc atcgtcatcc
     2761 ggtcggcctt cgttatcatc cccggcgtcc tcttctacct cctcgggatc gcaaacctgg
     2821 tcatgtacct ggtcgggatg cggatcattc ccggcctgtg agccccgaag ggggatttca
     2881 atcccccgag ggatgagaag tatctatttt tcgagccccg aaggggatct caatccccga
     2941 gggatgagaa aatcttattt tcgagcccgg ggagaggaag gaccaggtcc tgcccgtcgt
     3001 tttccggatc attcaccctg cccgagacag ggaatgcctc catctcctcc gccgggtac
//
LOCUS       DATGDI010000102        14325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_155328, whole genome shotgun sequence.
ACCESSION   DATGDI010000102 DATGDI010000000
VERSION     DATGDI010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 14325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14325
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155328"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..11443
                     /locus_tag="VKO45_03850"
     CDS             14..11443
                     /locus_tag="VKO45_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMA05040.1"
                     /translation="MGLARATLDDQGEAARAFGRATDLDPSSGPAWYGLGAASRRIGE
                     NQRALDALDHAVSLDPGAPAGLMEKGLTLGNLGRYAEALSAFDRVLEIAPGDRPALIA
                     RVETLASLERFGEGLAAAQRMLDRDPSLAAAWYLRGRCLSGLGRHGEAVESLGRALEL
                     DPDQASAWHQLGLVQEARGFFGDAADAFWRAGQIAPEEGSSWLHLGTNLIRETRWREA
                     LDALRRATARMPGDARAWHQLGLALRGTGRAGEAALAFEKALSIEPDRPETLAAQGQT
                     FMLAERFEEASRVLARAASLAPDDPDTWFRKGVSEDRTGRFAEAVASFDEAIARDPSN
                     LSAWFHRGLALRRSGDLPAALEAFDHVLSLEGEDREALFHRGAILRELGRPGEGLASL
                     EKGLSLEPGNAGAWFEKGLAERDLGKEEEAVASLRQALALDPRHARAWCALGIALMKE
                     GLTEKAVETLARAVVFDPGLTRAWLHLGVGLTGLRRFREAAEAIGNCLVQDEGDPDAW
                     YYQGFVLNRLGRHGKALESLDRALALDPASGKALLEKVRAEMAVLRYREALETLDRLN
                     GLEPEKAEAWYRRGSIMALLGIHEEALDHLATALRFDPSCRPALLQAALSQLELGNPP
                     EALKSLERAISLDPADPRPLFYRGVVLERMGRQPEAAASYDAVIRLRPADLAARERKG
                     QILLALGKHAEALSTAEEALGVSPENAGFQTLRGLSLLGLGRTGEAEEAFARSVEHEP
                     LRAGSWYHRGLALLDLGKNREAAEAFREALLLDPRNAPALFQAGVAYERLGEHEKALR
                     SLEEGLAIDPSPAGAWVTRGTAERELGRYEPAVSSFERAGELDPTNTEAWYGKALALR
                     LLGREQEALASLDTGLSLRPGDTVSWSLKGSLLRHLGRAAEAAEAFRHVTEGDPAAPQ
                     AWFQLGETLYELGQYDEALEATGKAVERDPGLTTAWFTRGMAFNRLGRYGEAVEAFRQ
                     VTIQNRDHGLAWYNMGWADRELRLYGEAVDAFDEAIRVDPRDAGSWYNRGLALHEMGR
                     FGDAVSSFDQVTTIDPENAQGWFRKALSLAALGRDEEAAEAFDRVIQLDPENHEPWHE
                     LGQIFLKLKGYDEALVCFDRALQISPEFHPSWFLRGETLSAMGRYPEAVEAYDRVLSF
                     EDGEPQAWLRKGLALRELKDDEGAAQALERFLLVEPGSAEAWYQKGLALEGKGKYATA
                     AEAFGRSLEIEPGRVDAWYQKGLALAGSGNPEEGIPAYQEAIRLDSTHALAWYSLGLA
                     LEQVKKFEKAMDAFARALQIQPKNLPAWFHRGLCEEEEGYFSDAVFSFEKALEIEPKD
                     VDSWFHKGTCLARAGRHQEAVNAFGNALLYDPENPRAWFHQGMSLLETGENEGAVASF
                     DRALGLDAGNPAAWYEKGVALESLGRREEALKAFSRSLDLDPERRDAWRHRGSLLFAG
                     KSLEAALESFDQVIRIDPKDALAWYNRGCILGDLGDAEGAVGSLDRALAIDPGNSRAW
                     NNKGAILFQEEQYAEAVDAFDHSLEIDSGNAGTWFNRGLALMERGEDEAAIGSFSRTL
                     DLDPGNAEAWFQKGKALERSGRPAEAVEALDEAIRLQPGHGQASFIRGLALERLQRFD
                     EAVHAFDRSLEADPRNAEALYHQGLALHALGMHHEAIEVFARAAELDPESAGAWYHMG
                     LALHTLGRPEEAVTALGQALVLQPGAPDAWYYHGISLSALRRHAEALDSFEKGLEVQG
                     RDGRLWHGKGVALAELGRNEEAARALGVAVEIDPEDERAWFTMGVSLHRLERYGEAVP
                     ALERSVSLDPANLEGWLLLGFDLRELGRNEEALAAAEGARRTDPGNPRAWTLLGIAQR
                     DLGRHAEALESLNHVIALNPGDAEAWFERGLVEAELGQEEEAEASLAKSLELEPSHPG
                     ALLGLGKILARAGSHGEAIECFRRLVRLDPGNGEAWYWMGMSLSLAGEEEEALDAFEK
                     TLKRDPGHAAAWYNRGCILNGLGRLEEAVDAFDHALSLEPGNAQAWNNKGGALLGLGR
                     AAEAAAAFREVVRAIPGHGPAWYNLGLSEMELGNPAPAAEAFEKALDIDPSLAEAWYQ
                     KGLSHERLGQQSEAVVSYDRAIALSPEDGPSWFRRGVALAALGKGTDAITSLERAATL
                     DPENAGAWYHLGLSLMGEKAYDRALESFTHALELRLEDGRLWFESGLALDALGHTEDA
                     LEALRRSVEIDPGDRESWYHRGLLEARTGHAREAVASFDRILDRDEGHAPSHHQKGLA
                     LLSLGEYDGAIAALSRAADLDPGNVDARFRRGSALSAAGRYEEALTAFDGALTLDPSH
                     GRAWAARGTTLAHLGRQAEAAEAFGKAVTLEPLEVPVHVALGKALMSLGRFEEAVHAL
                     ERGLLITPEHTGLWILKGTCEKELGKFGNAAASFGEAIGHEPGNPEPWYLLALTLEEA
                     GRPGDSLNALEGFLTLMPENPGAWMRAGDLLVTLGRGEEAIEAFERATTLAPKDAPSW
                     IRYGNSLLRASRFREAAEAYGKALNIEPGDAPAWFHRARALAEAGDDDGAAGAFARSL
                     EIDGRQAGAWAGKGRSLARLGRREEAVASFSRALDLDPALTAALQGRAEALHALGRNG
                     DAVPDLEEVLRREPGNASAWQDLSRVLRGLQRFPEAREAIGKALELDGKNVSAWAERG
                     FLEEALGEHTEAAGSYRTALSLSPEDPALWSGLGIAELSLQHHREAAEAFGESLGRSP
                     EDAAAWFHLGEVLLLLDHVDDAYDAFRKSADVRPDDARTWHFQGIALTRLVRLEEAIE
                     AFDRALEIDPGDAESWFARGIALYDLGRYEEAVESFGRTLALKGDRADAWFRTGLCHE
                     AQRDHTLAERAFGRAAGLSPDDGEAPYHRAIALMALGKNGEALECLERVLEIDPDQTD
                     AWFRKGLILAGLGRAEEAARAFDAVVRISPEHAGAWYQKGLALESAGKGEEASGCYEM
                     AVRSDPGFEQGWLHRGRILASLGKAGEGLSALDRALEIDPGDGEALFSRAGVLADLGR
                     PGEALEAYGRALERNPRSAEGHLRRGLLLLSLSRSEEAQGAFDKAIEADETDPRAWSE
                     KGAVLAGTGKYGEAVGAYDRALGLDPESPGTLRRRGVSLLALGRIPEAVADLEKAAAL
                     TGEDAGTWLDLGMARIRGRLFEGAAEALDRSLALDPGNPRAWCASGIALARLGRLEEA
                     ASALGRALAADDGLEAAWFQLGLAQEGLNRYEEAVESFRRGLKIDVRNEEAWYHAGGA
                     LYHLGREEEALRAFERATKLSPRDPRPWYNEGCIFSERGEFGAAVEAFDRSLEADPRF
                     AAALNNRGSALFELGQYGEAEASFDRSLEIEPGNPAVWYNKALIQKQRGAQDAALESL
                     ARVLELDPGHAGALHTRGLLLHALGRDGEALEAYDRALSLNSGLVHVWYDRGIVLEER
                     GGTEEAIGSFRHAADLDPGDALAWYRMGILLARQGRHTEAREALVRSLAADPSRADAW
                     YRLGLSLRETRGEEEAVGAFDHALEIEPRHGGAWYNRGLALFALARFVESVTSFERAL
                     EIDPADAGAWHNRGYACLTIRNHAEALASFDKALELDGGNPLSWTYRGLALEGLGRRE
                     EGFASHRKALEIDPANRIALANQSWAFYGERQLDLALEGFERILSRDPADPAAWLGKA
                     AVLEGQGKTEEALKAYDRAVETGPDDARAWNSRGALLYRMGRIPEAIHSFENAVDADP
                     RLAAAWNGLAAVKGGLGRHDEALGAAEKALEISPDDARAWNNRGGSLFQLGRYEEALA
                     CFEKAIALDPGYASPWMNKGQALARLKRRREARSAMKKAKELDPTLGEPAGKT"
     gene            11458..12147
                     /locus_tag="VKO45_03855"
     CDS             11458..12147
                     /locus_tag="VKO45_03855"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HMA05041.1"
                     /translation="MTRPYVIVNMAMSADGKISSVERRQVRISGAADRERVDRLKAEC
                     DAVMVGIGTVLADNPSLTVKSAALRGARARAGKPENPVRIVVDSRGRTPPSADILHRG
                     PGERIIAVSGRAGPEATLRYHDLATVIPAGEGDEVELHLLMGRLHELGIGRLMVEGGG
                     SLVASLFARGLVDEFLTYVGNLVIGGKDAPTPADGPGFPNDNEFVRLSLMDVSELDEG
                     VLLRWRVKNHR"
     gene            12123..13418
                     /locus_tag="VKO45_03860"
     CDS             12123..13418
                     /locus_tag="VKO45_03860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMA05042.1"
                     /translation="MAGEKPPVDRRVLVDLAVVLICYTGVAALIAILMYLVSGSGSTW
                     LTFDAARFHSMAEVIIGGFKPYIDFVDPKPPLLFFMVSAMDLIAPAGSVDILVMSAVN
                     VLCALLIYWIGRNDYGNISGFSAGLLFLVAAVFVQGYFLFSEQFAVLFLILAFMLVRK
                     DHWAAAGLCLGLAMGFKQYAFLAIPPLLFLARVKGSERVVRYIEFIIPALLVFFALFG
                     ALYLAYGPDATRSAINWTFGIAPSYVTGHLIEVPAYQPDNVLAFTANLLGSVMMVLPT
                     LLFAMTSIWRRGLRTDDEYAFGLFIIFFLGTLLIRQYLHYWILMLPFLVILACREFAV
                     GSARESDESPEGSVLHRLAGFAREVGSSPEAPGIDDGPDSASRIVFSAPVPGSPAPMG
                     DAREPGGLIPYRREEGKPGGSEKKEETTGPEQGKDEDSR"
     gene            complement(13453..13947)
                     /locus_tag="VKO45_03865"
     CDS             complement(13453..13947)
                     /locus_tag="VKO45_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05043.1"
                     /translation="MNGRIALIPILLVLAAALGAGCTGTPGPTTPTPTPGTTVPATTM
                     ETIPVTTPADLVPGPTVTVPPVWAVAVDVLRDSNTYTRKITVIFQGGKGQSATQRVDV
                     VVTHDDGSVETKSITRPESGSIIAGSSVNFTGTLLDRIEVTVWLNGIPYKIYDKVLPL
                     ASRS"
     gene            14116..>14325
                     /locus_tag="VKO45_03870"
     CDS             14116..>14325
                     /locus_tag="VKO45_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HMA05044.1"
                     /translation="MNLRRPNANEALGTSNRSRNVAPMSGICTRCLDGCKGMCDIWLS
                     SFRGREVLYPGPFGEMTAGADKDYPV"
BASE COUNT         2147 a         4981 c         5080 g         2117 t
ORIGIN      
        1 ggcatggtac cagctggggc ttgcccgggc aaccctcgat gaccagggcg aggccgcccg
       61 ggccttcggg cgggcgacgg atctcgaccc gtcctccggc ccggcctggt acgggctggg
      121 ggccgcctcc cgacgaatcg gagagaacca gcgggccttg gacgccctcg atcacgccgt
      181 ctccctggac ccgggagcgc cggccggctt gatggagaag gggctcaccc tcggaaacct
      241 cgggcggtac gcggaggccc tttcggcctt cgaccgggta ctcgagatcg ctcccgggga
      301 cagacccgct ctcatcgccc gggtggagac cctcgcctcc ctggagcggt tcggggaggg
      361 gctcgccgcg gcccagagga tgctcgaccg ggacccatcc ctcgcggcgg cctggtacct
      421 caggggccgg tgcctctcgg ggctgggccg gcacggtgaa gcggtggagt ccctcgggag
      481 ggccctcgag ctcgacccgg accaggcctc cgcgtggcac cagctgggcc ttgtccagga
      541 ggcacggggc ttcttcgggg acgcggccga tgctttctgg agggccgggc agatagcgcc
      601 ggaggaaggg tcgtcctggc tccacctggg tacaaacctc atccgggaga cccgctggag
      661 ggaggccctg gatgccctca ggcgggcgac cgcccggatg ccgggggatg cccgcgcctg
      721 gcaccagctc ggcctcgccc tcaggggaac ggggagggca ggggaggccg ccctcgcctt
      781 cgagaaggcc ctctccatcg agccggaccg ccccgagacc ctcgcggccc aggggcagac
      841 cttcatgctc gccgagcgat tcgaggaggc ctcgcgggtc ctcgcccggg ccgcctccct
      901 cgcgcccgac gaccccgaca cctggttccg gaagggggtc tccgaggacc ggaccgggag
      961 gttcgcagag gcggtggcgt cctttgacga ggccatcgcc agggacccct cgaacctgtc
     1021 agcctggttc caccggggcc tcgccctccg gcgttcgggc gacctcccgg ccgccctcga
     1081 ggccttcgac cacgtcctct ccctcgaggg ggaggaccgg gaggccctgt tccaccgggg
     1141 cgcgatcctc cgcgagctcg gccggcccgg tgagggcctc gcgtccctgg agaagggcct
     1201 cagcctggag cccgggaacg ccggggcatg gttcgagaag gggctcgcgg agcgggacct
     1261 cgggaaggag gaagaggcgg tcgcatccct ccgccaggcc ctcgccctcg acccacggca
     1321 cgcccgggcc tggtgcgccc tcggcatcgc cctcatgaag gaggggttaa ccgagaaggc
     1381 cgtcgagacc ctggcccggg ccgtggtctt cgacccgggc ctcacccggg cctggctcca
     1441 cctcggggtc gggctcacgg gcctgcgccg gttccgggag gccgcggagg ccatcgggaa
     1501 ctgcctcgtc caggatgaag gggacccgga cgcctggtac taccaggggt tcgtcctgaa
     1561 ccggctcggc aggcacggga aggccctcga atccctggat cgcgccctcg ccctcgaccc
     1621 ggcgagcggg aaggccctcc tcgagaaggt gcgggccgag atggccgtcc tccggtaccg
     1681 ggaggccctc gagaccctcg accggctcaa cggcctcgag cccgagaagg ccgaggcctg
     1741 gtaccgtcgc gggtccatca tggccctcct cgggatccac gaagaggccc tcgatcacct
     1801 ggcgacggcc ctccggttcg acccgtcctg ccggccggcc cttctccagg cggccctctc
     1861 ccagctggag ctcgggaatc cccccgaggc cctgaagtcc ctggagaggg ccatctctct
     1921 tgatcccgcc gacccccggc ccctcttcta ccggggggtc gtcctcgaga ggatgggcag
     1981 gcagcccgag gcggcagcgt cctacgacgc ggtaatccgg ctccggccgg cggacctcgc
     2041 ggcccgggag cggaaggggc agatcctcct cgccctgggt aagcatgccg aggccctatc
     2101 cacggcggag gaggccctcg gggtgagccc ggagaacgcc gggttccaga ccctccgggg
     2161 cctttccctc ctcgggctgg ggaggaccgg ggaggccgag gaggccttcg cccgttcggt
     2221 ggagcatgag ccgctccggg ccgggtcctg gtaccaccgg ggcctcgccc tgctggacct
     2281 cgggaagaac cgggaggcgg ccgaggcctt ccgtgaggcc ctcctgctgg acccgcggaa
     2341 cgcccccgcc ctgttccagg ccggggtcgc ctacgaacgg ctcggggagc atgagaaggc
     2401 cctccggagc ctcgaggagg ggctggccat cgacccctcc cctgccgggg cctgggtcac
     2461 ccggggcacc gccgagaggg agctcgggag gtacgagccc gcggtctcct ccttcgagag
     2521 ggccggtgag ctcgatccca cgaacaccga ggcatggtac gggaaggccc tcgccctccg
     2581 gctcctgggg agggaacagg aggccctcgc ctccctcgac acgggcctct ccctgcggcc
     2641 gggcgacacc gtgtcctgga gcctgaaggg ctccctcctc aggcacctgg gccgtgccgc
     2701 cgaggcggcc gaggccttcc ggcacgtgac cgagggggat cccgccgccc cccaggcctg
     2761 gttccagctg ggcgagaccc tgtacgagct cgggcagtat gacgaggccc tggaggccac
     2821 cgggaaggcc gtggagcggg accccggcct caccaccgcg tggttcaccc gggggatggc
     2881 cttcaaccgg ctcgggaggt acggggaggc ggtcgaggca ttccgccagg tgaccatcca
     2941 gaaccgggac cacggactcg cctggtacaa catggggtgg gcggaccggg agctgcgtct
     3001 ctacggggag gccgtggatg ccttcgacga ggccatccgg gtggaccccc gggacgcggg
     3061 ttcctggtac aaccggggcc tggccctcca cgagatgggg aggttcgggg atgcggtctc
     3121 ctccttcgac caggtcacca ccatcgaccc ggagaacgcc cagggctggt tccggaaggc
     3181 cctctccctc gctgccctgg gccgggacga ggaggcggcc gaggccttcg accgggtgat
     3241 ccagctcgac ccggagaacc acgagccctg gcacgagctg gggcagatct tcctcaaact
     3301 gaaggggtat gacgaggccc tcgtctgctt cgaccgggcc ctccagatca gcccggagtt
     3361 ccacccgtcc tggttcctcc ggggcgagac cctctctgcc atgggccgct accccgaggc
     3421 ggtggaggca tacgaccggg tcctttcctt cgaggacggg gagccccagg cctggctccg
     3481 gaagggcctc gccctccggg agctcaagga cgacgagggg gccgcccagg ccctggagcg
     3541 cttccttctc gtcgagcccg gatctgccga ggcctggtac cagaagggcc tcgccctcga
     3601 ggggaagggg aagtacgcca ccgccgcgga ggccttcggg aggtccctcg agatcgagcc
     3661 gggccgggta gacgcctggt accagaaggg cctcgccctg gccgggtccg ggaaccccga
     3721 ggaggggatc cccgcctatc aggaggccat ccgcctcgat tccacccatg ccctcgcatg
     3781 gtacagcctt ggcctcgccc tggagcaggt gaagaagttc gagaaggcga tggacgcctt
     3841 tgcccgggcc ctccagatcc agccgaagaa cctccccgcc tggttccacc gggggctctg
     3901 cgaggaggag gaggggtact tttccgatgc cgtcttctcc ttcgagaagg ccctggagat
     3961 cgagcccaag gacgtggatt catggttcca caaggggacc tgccttgccc gggcgggccg
     4021 gcaccaggag gcggtgaacg ccttcgggaa tgccctcctc tatgatccag aaaacccgcg
     4081 ggcctggttc caccagggca tgtcccttct cgagacaggg gagaacgagg gggcggtcgc
     4141 ctcctttgac agggccctcg gcctcgacgc ggggaacccg gccgcctggt acgagaaggg
     4201 ggtggccctg gagagcctcg gccggcggga ggaggccctg aaggcctttt cccggtccct
     4261 cgatctcgac ccggagagaa gggatgcctg gaggcaccgg ggctccctcc tcttcgccgg
     4321 gaagtccctg gaggccgccc tcgagtcctt tgaccaggtg atccgcatcg acccgaagga
     4381 tgccctcgcc tggtacaacc ggggctgcat cctgggcgac ctcggggatg ccgagggcgc
     4441 cgtcgggtcc ctggaccggg ccctcgccat cgacccgggc aattcccgcg catggaacaa
     4501 caagggggcc atcctcttcc aagaggagca gtacgcggag gcggtggatg ccttcgatca
     4561 ctccctcgag atcgattcgg ggaacgcggg gacctggttc aaccgggggc tcgccctcat
     4621 ggaacgggga gaggacgagg cggccatcgg gtccttttcg aggaccctcg acctggatcc
     4681 cgggaacgcc gaggcatggt tccagaaggg gaaggccctc gaacggtcgg ggcggcccgc
     4741 cgaggcggtg gaggccctcg acgaggccat ccggctgcag ccggggcacg gccaggcctc
     4801 gttcatccgg ggcctcgccc tggagcggct ccagcggttc gacgaggcgg tgcatgcctt
     4861 cgaccggtcc ctggaggcgg acccccggaa cgccgaggcc ctctaccacc agggactcgc
     4921 cctccatgcc ctgggaatgc accacgaggc catcgaggtc tttgcccggg ccgccgagct
     4981 ggatcccgaa tcggccgggg cctggtacca catgggcctc gcccttcaca ccctggggag
     5041 gcccgaggag gccgtgaccg ctctcggcca ggccctcgtt ctccagccgg gcgccccgga
     5101 cgcatggtac taccacggca tctccctctc cgccctccgc cggcatgccg aggccctgga
     5161 ttccttcgag aaaggtctcg aggtccaggg aagggacggg aggctctggc acgggaaggg
     5221 ggtcgccctc gcggagctcg ggagaaacga ggaggcggca cgtgccctgg gagtcgcggt
     5281 ggagatcgat ccggaggacg agcgggcctg gttcaccatg ggggtctccc tccaccggct
     5341 cgaacggtac ggggaggcgg tcccggccct cgaacggtcg gtctccctcg acccggcgaa
     5401 cctggagggc tggctcctcc tcggcttcga cctccgcgag ctcggccgga acgaggaggc
     5461 cctcgccgcg gccgaggggg cccggcggac cgacccgggg aacccccggg catggaccct
     5521 cctcgggatc gcccagcggg acctcggccg gcacgccgag gccctcgagt ccctgaacca
     5581 cgtcatcgcc ctcaacccgg gggatgccga ggcctggttc gagagagggc tcgtcgaggc
     5641 cgagctcggt caggaggagg aggcggaggc ctccctcgcg aagtccctcg agctcgagcc
     5701 atcccacccg ggggccctcc tcgggctcgg gaagatcctg gcccgggccg ggtcccacgg
     5761 cgaggccatc gagtgcttcc ggcggctcgt ccggctcgac cccgggaacg gggaggcctg
     5821 gtactggatg ggaatgagcc tctccctcgc cggtgaggag gaggaggccc ttgacgcctt
     5881 cgagaagacc ctgaaacggg accccggcca tgcggcggcc tggtacaaca ggggctgcat
     5941 cctgaacggc ctcggacggc tcgaagaagc cgtcgatgcc ttcgaccatg ccctctccct
     6001 tgaacccggg aacgcccagg cctggaacaa caagggcggg gctctcctcg ggctcgggcg
     6061 ggccgccgag gccgcggccg ccttccggga ggtcgtgaga gccatcccgg gccatgggcc
     6121 ggcctggtac aacctcggtc tctccgagat ggagctcgga aaccccgctc ccgctgccga
     6181 ggccttcgag aaggccctgg acatcgaccc ctccctcgcg gaggcgtggt accagaaggg
     6241 gctctcccac gagaggctcg ggcaacagtc cgaggccgtc gtatcctatg accgggccat
     6301 cgccctctcc cccgaggatg gtccgtcctg gttccggagg ggtgtcgccc tcgcggccct
     6361 cggaaaggga accgatgcca ttacgtccct cgagcgggca gccaccctcg acccggagaa
     6421 cgcgggggcc tggtaccacc tgggcctctc cctcatgggg gagaaggcct acgaccgggc
     6481 cctggaatcc ttcacccatg ccctcgagct ccggctcgag gacgggaggc tgtggttcga
     6541 gagcggcctt gccctcgatg cactgggcca cacggaggat gcactcgagg ccctccggag
     6601 atcggtggag atcgaccctg gggaccggga gtcatggtac cacagggggc tcctggaggc
     6661 acggaccggg catgcccggg aggcggtggc ctcctttgac cggatcctcg acagggacga
     6721 ggggcatgcc ccgtcccacc accagaaggg gctcgccctc ctctccctcg gggagtacga
     6781 cggggccatc gcggcccttt cgagggctgc cgatctcgat ccggggaacg tggatgcccg
     6841 gttcaggagg ggatcggccc tgtccgccgc cggaaggtac gaggaggccc tcacagcctt
     6901 cgacggggcc ctcaccctcg acccgtctca cgggcgggcg tgggccgcga ggggaaccac
     6961 cctcgcacac ctcgggcggc aggcggaggc cgcggaggcc ttcgggaagg ccgttaccct
     7021 cgaacccctg gaggtcccgg tccacgtcgc cctcgggaag gccctcatga gcctgggccg
     7081 gttcgaggag gcggtgcatg ccctcgagcg ggggctcctc atcacccccg agcataccgg
     7141 cctctggatc ctcaagggga cctgcgagaa ggaactcggg aagttcggga acgccgccgc
     7201 atccttcggg gaggccatcg gccacgagcc cgggaatccc gagccctggt accttctcgc
     7261 cctcaccctc gaggaggcgg gccggcccgg ggattctctg aacgccctcg aggggttcct
     7321 caccctcatg cccgagaacc ccggggcatg gatgcgggcg ggggaccttc tcgtcactct
     7381 cggtcgcggg gaggaggcca tcgaggcctt cgagcgggca acgaccctcg ccccgaagga
     7441 tgccccctcc tggatccggt acgggaattc cctcctcagg gcctcccggt tccgggaggc
     7501 cgcagaggcg tacggaaagg ccctgaacat cgagccgggg gatgccccgg cctggttcca
     7561 ccgcgccaga gccctcgcgg aggccgggga tgacgacggg gccgccggcg cttttgcccg
     7621 ttccctggag atcgacggga ggcaggccgg ggcctgggcc gggaagggaa ggagtctcgc
     7681 gcggctggga aggcgggagg aggccgtcgc ctccttctcc cgggccctcg atctcgaccc
     7741 ggccctcacc gctgccctcc agggccgggc ggaggccctc cacgccctgg gcaggaacgg
     7801 ggacgccgtc ccggacctcg aggaggtcct gcgccgggag ccgggcaacg cctctgcctg
     7861 gcaggatctc tcccgggtgc tcaggggcct gcagagattc cccgaggccc gggaggccat
     7921 cgggaaggcc ctggaactgg acgggaaaaa cgtctcggca tgggccgaga gggggttcct
     7981 cgaggaggcc ctcggcgagc atactgaggc tgccgggtcc taccggaccg ccctctccct
     8041 ctcccccgag gatccggccc tctggtccgg gctcgggatc gccgagctct ccctccagca
     8101 ccaccgggag gccgccgagg ccttcgggga gagcctcggg cgctcccccg aggatgcggc
     8161 agcctggttc cacctggggg aggtcctcct cctcctcgat cacgtcgacg acgcctacga
     8221 tgccttcagg aagagcgccg atgtccggcc ggacgatgcc cggacctggc acttccaggg
     8281 gatcgccctc acccgcctcg tccggctcga ggaggcgatc gaggccttcg accgggccct
     8341 ggagatcgac ccaggcgatg ccgagtcatg gttcgcccgg gggatcgccc tgtatgacct
     8401 cgggaggtac gaggaggcgg tggagtcctt cgggcggacc ctcgctctca agggggaccg
     8461 ggcggatgca tggttccgga cgggcctctg ccacgaggca cagagagacc ataccctcgc
     8521 cgagcgtgcc tttggccggg cggccgggct ctcgcccgat gacggggagg cgccctacca
     8581 ccgggccatc gccctcatgg ccctcgggaa aaacggggag gccctcgaat gcctggagcg
     8641 ggtcctcgag atcgacccgg accagaccga tgcctggttc aggaaggggc tcatcctcgc
     8701 cggtctcggc cgggcagagg aggcggcccg tgccttcgat gccgtggtcc gcatctcccc
     8761 ggagcatgcc ggggcctggt accagaaggg cctcgccctg gaatccgccg ggaaggggga
     8821 ggaggcctcg ggatgctacg agatggcggt gaggtcggat ccgggcttcg aacagggatg
     8881 gctgcaccgg ggccggatcc tcgcgtccct ggggaaggca ggggaaggcc tctccgccct
     8941 ggaccgggcc ctggagatcg acccgggcga cggcgaggcc ctgttctccc gtgcgggtgt
     9001 cctcgcggac ctgggccggc ccggagaggc cctcgaggcc tacggccgtg ccctggagcg
     9061 gaatccccga agcgcggagg gccatctccg gagagggctc ctcctccttt ccctctcccg
     9121 ctccgaggag gcccaggggg ccttcgacaa ggccatcgag gcggacgaaa ccgacccgcg
     9181 ggcctggtct gagaagggtg cggtacttgc cgggacgggg aaatacgggg aggcggtcgg
     9241 ggcgtacgac cgggccctcg gcctcgaccc cgaaagcccc ggcaccctca ggagaagggg
     9301 agtctccctt ctcgccctcg gcaggatccc ggaggcggtt gcggacctgg agaaggcggc
     9361 ggccctcacc ggggaggacg cgggcacctg gctcgacctg gggatggccc ggatccgggg
     9421 gagactcttc gagggggccg cggaggccct tgatcggtcc cttgccctcg atcccgggaa
     9481 tccccgggcc tggtgcgcga gcggcatcgc cctcgcccgc ctcggccggc tcgaggaggc
     9541 ggcctctgcc ctcggccggg ccctcgcggc tgacgacggc ctcgaggccg cctggttcca
     9601 gctgggcctc gcccaggagg gcctcaaccg gtacgaggag gcggtggagt ccttccggag
     9661 ggggctcaag atcgatgtcc ggaacgagga ggcctggtac catgccggcg gggccctcta
     9721 ccatctcggc agggaggagg aggccctccg cgccttcgag cgggccacga agctctcccc
     9781 ccgggacccg aggccctggt acaacgaggg ctgcatcttc tccgagcggg gggagttcgg
     9841 ggccgcggtg gaggccttcg accgttccct cgaggccgac ccccggttcg cggccgccct
     9901 gaacaaccgg gggagcgccc tcttcgagct cgggcagtac ggggaggcgg aggcctcctt
     9961 cgaccgctcc ctcgagatcg agcccggaaa cccggcggtc tggtacaaca aggccctcat
    10021 ccagaagcag aggggggctc aggatgccgc tctcgagtcc ctcgcccggg tgcttgagct
    10081 cgatcccggc catgccgggg cgctccacac ccggggtctc cttctccacg ccctcggccg
    10141 ggacggcgag gccctggagg cgtacgaccg ggcgctctcc ctcaattccg ggctcgtcca
    10201 cgtctggtac gaccggggga tcgtcctcga ggagagaggc gggacggagg aggccatcgg
    10261 gtccttccgg cacgccgcgg acctggaccc cggggatgcc cttgcctggt accggatggg
    10321 aatcctcctc gcccggcagg ggcggcacac cgaggcccgg gaagccctcg tgaggtccct
    10381 cgcggcggac ccgtcccggg cggacgcgtg gtaccggctc gggctctccc tgcgggagac
    10441 gcggggggag gaggaggccg tcggggcctt tgaccatgcc ctcgagatcg agccccggca
    10501 cgggggcgcg tggtacaacc gcggcctcgc cctcttcgcc ctcgcccggt tcgtcgagtc
    10561 cgtcacctcc ttcgagcgtg ccctggagat cgacccggca gacgccgggg cctggcacaa
    10621 ccggggctac gcctgcctga ccatccggaa ccatgccgag gccctcgcct cctttgacaa
    10681 ggccctggag ctggacggcg ggaacccgct ctcctggacc taccggggtc tcgccctcga
    10741 ggggctcggc cggcgggagg agggttttgc ctcccaccgg aaggccctcg agatcgaccc
    10801 ggcgaaccgc atcgccctcg cgaaccagtc ctgggccttc tacggagaga ggcagctcga
    10861 cctcgccctc gagggattcg agaggatcct ctcccgggac ccggccgacc cggcggcgtg
    10921 gctggggaag gccgcggtgc tcgaggggca ggggaagaca gaggaggccc tgaaggccta
    10981 tgaccgggcc gtggagaccg ggccggacga tgcccgggcc tggaacagcc ggggggccct
    11041 cctctaccgg atgggtcgga tcccggaggc catccattcc ttcgagaacg cggtggacgc
    11101 ggatccccgg cttgccgccg catggaacgg cctcgcggcc gtgaagggtg gcctcgggag
    11161 gcacgacgag gccctcgggg ccgccgaaaa ggccctcgag atcagcccgg acgatgcccg
    11221 ggcctggaac aacagggggg gttccctctt ccagctggga aggtacgagg aggccctcgc
    11281 ctgcttcgag aaggccatcg ccctcgaccc cgggtacgca tcgccctgga tgaacaaggg
    11341 tcaggccctc gcgaggctga agaggcggcg ggaggccagg tccgcgatga agaaggcgaa
    11401 ggagctcgac cccacgctcg gcgagcccgc gggaaagacc tgaccatggg tgatcggatg
    11461 acgcggccct acgtcatcgt gaacatggcc atgagtgccg acggaaagat ctcctccgtc
    11521 gagaggaggc aggtgcggat cagcggggcg gccgaccggg agagggtgga ccggctgaag
    11581 gcggagtgcg atgcggtgat ggtcgggatc ggtacggtcc ttgccgacaa cccgtccctc
    11641 acggtgaagt ccgcggccct ccggggggcc cgggccaggg ccgggaagcc ggagaacccg
    11701 gtgaggatcg tcgtggacag ccggggaagg acccccccct ccgccgacat cctccacagg
    11761 ggacccggtg agcggatcat cgcggtctcg ggcagggcgg ggccggaggc cactctccgg
    11821 tatcacgacc tcgccacggt cattcccgcc ggggaggggg acgaggtgga gctccacctc
    11881 ctcatgggcc ggctccacga gctcgggatc gggcggctca tggtggaggg cggggggtcc
    11941 ctggtcgcct cccttttcgc ccggggcctc gtggacgagt tcctcaccta tgtcgggaac
    12001 ctggtcatcg ggggaaagga cgccccgacc cccgcggacg ggccgggatt tcccaatgac
    12061 aatgaatttg tgaggctgag tctcatggat gtgagtgagc ttgacgaagg ggtgctcctg
    12121 cggtggcggg tgaaaaacca ccggtagacc ggcgcgttct cgtggacctg gcggtcgtcc
    12181 tcatctgtta taccggtgtt gccgccctca tcgccattct catgtatctg gtgagcggga
    12241 gcggctccac ctggctcacc ttcgatgccg cccggttcca ttccatggcc gaggtcatca
    12301 tcgggggctt caagccctac atcgacttcg tggacccgaa gccgcctctc ctcttcttca
    12361 tggtctcggc aatggacctc atcgcaccgg ccgggagcgt ggacatcctc gtcatgtcgg
    12421 cggtgaacgt cctctgcgcc ctcctcatct actggatcgg gaggaacgac tacgggaaca
    12481 tctccggctt ctctgcgggc ctcctcttcc ttgtcgcggc cgtcttcgtc caggggtact
    12541 tcctcttctc cgagcagttc gcggtcctct tcctcatcct cgccttcatg ctcgtccgca
    12601 aggaccactg ggctgccgcg ggcctctgcc tcggcctcgc catgggcttc aagcagtacg
    12661 ccttcctcgc catcccgccc ctcctcttcc tcgccagggt gaagggcagc gagcgggtgg
    12721 tccggtacat cgagttcatc attcccgcgc tcctggtgtt cttcgccctc ttcggggccc
    12781 tgtacctcgc gtacggcccc gatgccacga ggagcgccat caactggacc ttcgggatcg
    12841 ccccctccta tgtcacgggg cacctcatcg aggtcccggc gtaccagccc gacaacgtcc
    12901 tcgcctttac ggcaaacctc ctcggatcgg tgatgatggt cctccccacc cttctcttcg
    12961 ccatgacctc catctggagg cggggcctca gaaccgacga cgagtacgcc ttcggcctct
    13021 tcatcatctt cttcctcggg acgctcctca tccgccagta cctccactac tggatcctca
    13081 tgctcccctt cctcgtcatc ctcgcctgca gggagtttgc cgtcgggtcg gcccgggagt
    13141 cggacgagag ccccgaggga tcggtcctcc accgccttgc cgggttcgcc cgcgaggtcg
    13201 gctcctcccc ggaggccccg ggcatcgacg acgggccgga ctctgcctcg cggatcgtct
    13261 tctcggcccc ggtccccggg tcgcctgccc cgatgggcga tgcccgggag ccgggcggcc
    13321 tcatcccgta ccggcgggag gagggcaaac cgggtggcag tgaaaagaaa gaggagacaa
    13381 ccggcccgga acagggaaag gacgaggatt cccggtaacc ggccggaaca ctactaaaaa
    13441 atttgtattt tttcaggatc tggacgccag ggggaggacc ttatcgtaga tcttgtacgg
    13501 gatgccgttc agccagaccg tgacctcgat ccggtcgagg agggtccccg tgaagttcac
    13561 cgagcttccc gcgatgatgg agcccgattc gggccgggtg atggacttcg tctccacgct
    13621 cccgtcgtca tgggtgacca cgacatccac ccgctgggtg gcgctctgcc ccttcccgcc
    13681 ctggaagatc acggtgatct tccgggtgta ggtgttcgag tccctgagca catcgacggc
    13741 aaccgcccag acgggaggca cggtcacggt cggccccggc acgaggtccg ccggggtcgt
    13801 gacagggatg gtttccatcg tggtagccgg tacggtcgtc ccgggtgtgg gggtgggggt
    13861 cgttggtccc ggggttcccg tgcagcctgc cccgagggcg gccgcaagga caaggagtat
    13921 gggaatgagg gctatccttc cgttcatacg cgaaggttgg gactgtgcat acttgaggat
    13981 tgtgcctgac cacaaatccg ttataggggg aggccttggg gaggccggaa agaccgtcgg
    14041 gccaggccgc aggtgatgca gacacccggg tccatatcga aaatcattta acagggggaa
    14101 agaaaccgta ttctcatgaa cttaagacga ccaaatgcca acgaggccct gggaacctcg
    14161 aaccggtcac ggaacgtcgc tcccatgtcc ggcatctgca cccgctgcct tgacggatgc
    14221 aaggggatgt gcgatatctg gctctcgagt ttccgcgggc gcgaggtgct ctaccccggt
    14281 cccttcggcg agatgaccgc gggggccgac aaggactacc ccgtc
//
LOCUS       DATGDI010000103         7483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_242599, whole genome shotgun sequence.
ACCESSION   DATGDI010000103 DATGDI010000000
VERSION     DATGDI010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242599"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VKO45_03875"
     CDS             complement(<1..422)
                     /locus_tag="VKO45_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HMA05045.1"
                     /translation="MSDFERILIANRGEIAIRVMRACRELGIETVAIYSDPDENALHV
                     KYADEAFCVGEAHPSKSYLNMERIVEVAKKAGAGAIHPGYGFLAENSAFAKLVESEGL
                     IFIGPHWKTIEAMGSKIGSKRMMRKAGVPVLPGTEEGTT"
     gene            complement(419..2149)
                     /locus_tag="VKO45_03880"
     CDS             complement(419..2149)
                     /locus_tag="VKO45_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/oxaloacetate carboxyltransferase"
                     /protein_id="HMA05046.1"
                     /translation="MHRVHITDTTLRDAHQSLIATRLRTEDSLPLARALEKAGFFSVE
                     AWGGATFDTAIRFCNDDPWDRLSAFAEALPKTPIQMLLRGQNLVGYRHYPDDVVERFV
                     AAAHRRGVGIFRVFDALNDVRNMKKAMKEVKKAGAHLQGAICYTTSPVHSVKGFIAMA
                     KELQGHDCDSICIKDMAGLIKPEPARELVTGIKKATGLPVDLHSHSTAGLAPMSYQAA
                     IEAGVDILDTAMSPFALGTSQPPTESVVASVEGTDRDTGIDLHVLREARNVCLSIREK
                     YLGILDPISERVDSDVLLYQLPGGMISNLVSQLKEQDALNRREDVHQEIPRVREDLGY
                     PPLVTPTSQIVGTQAVFNVLIGGERYTNVTKEVKDYLRGLYGKPPGPVNEEVRRKIIG
                     NEKVVTVRPADLLEPVYDSMKKEAVSLGLVKKEEDVLTYILYPAVAPAFLKGEKRPEI
                     LPPKMSAAAQVPDVPSAMEVEVDGEVYSVRIVSVAGAAVVAKAGAASAGKAPRGEIEG
                     GIRSNMQGMVLKVLVRVGEAVKKGDPLMVLEAMKMENPIASPRDGKVTEIFVDTGDVV
                     ASGDVLLVVR"
     gene            complement(2241..2867)
                     /locus_tag="VKO45_03885"
     CDS             complement(2241..2867)
                     /locus_tag="VKO45_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05047.1"
                     /translation="MQDPRLILLSTVALSVAAFAGVTGALLAIGWWAVATPRHLPLGR
                     WRTLLGILLFVLATAVLAAFSGGNGLSYLVRISAVLLIATWAYGSRKGGEFLDMGVWA
                     LGDRTGFELGLVAEMAVGSLEVLDRDLGDLLLAIRQKAGRLTIMSLVPALGLLVKREL
                     ERARDQGDILAVRGYRDGGSLCPRFAQGPWDGVKALSAVGIALLALLL"
     gene            complement(2849..3547)
                     /locus_tag="VKO45_03890"
     CDS             complement(2849..3547)
                     /locus_tag="VKO45_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HMA05048.1"
                     /translation="MRLSLLDVTLRRGAFRLRAAGTFSEGIHLVTGPVGSGKSTLALA
                     LAGALRPETGEIAREGIGSVTLGLPFPGCQVTGSTLEAEAGTWGVAPGPVLAAAGLEG
                     RGREDPLRCSQGELKRLVLSCILARAGDLLILDEPYGLLDCAWKSVLSGRLAERRRGI
                     TILLTHEDENLPRVDEIWEIRDGVLFPLGRVPEAIARWSRPPAYLRAALESGALPVNI
                     TPGDARDAVCRTRG"
     gene            complement(3544..4212)
                     /locus_tag="VKO45_03895"
     CDS             complement(3544..4212)
                     /locus_tag="VKO45_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HMA05049.1"
                     /translation="MIRITGLSHRILSIPDLTLPSGIVAVIGPNGGGKTTLLRLVAGI
                     DIPSEGEILVEGTPPRGRETGWVGENPGETMVFTRVYDEIASPLRFRRDPCSRVDERV
                     TEVAEKLGMSDLLGRETGTLSSGEKARVALAAALAADPDLLVLDEADSNLDQESTIGM
                     MDTIRRSSCSQVIEATQRMGLASSADHIVFLEGGRVVHSGRPAAVLAALSGTPFSHPR
                     EGCL"
     gene            complement(4209..4718)
                     /locus_tag="VKO45_03900"
     CDS             complement(4209..4718)
                     /locus_tag="VKO45_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HMA05050.1"
                     /translation="MHGNPERTRLLAETAAFIALIAAGAWVAIPVGPVPVTLQTFFLL
                     LAAPVMKRRAIIPAALYLLLGAANLPVFHSGLAGIGVLLGPSGGFLAGFIPAAAVAGF
                     AYGSPSRILRGAGLAAGTLVIYVCGVAWLLFSAGLSLPAAVLIGVVPFLPGDILKGAG
                     VFLLGERLP"
     gene            complement(4723..5703)
                     /locus_tag="VKO45_03905"
     CDS             complement(4723..5703)
                     /locus_tag="VKO45_03905"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HMA05051.1"
                     /translation="MDDPAFKVLRVLDEEGGSAPAESLSASLGIPRSRVSESVDRLRH
                     LGYAIAEPRKDLYQLTGRSTRLLPYEIERHLGTKVIGRRMKYLEATESTTWIARDLLC
                     QQDPARLHGMVIIAEEQTGGQGRLGRTWASPPGGIWTTTILAPKIPIDRVFMVTMAAA
                     IAVARALRKDLDLGALIKWPNDILLGDRKVAGLLLELSAENSEVKYALLGLGIDANIS
                     LDHLPADLRATVTSLSAELGREVDRPALFASVLREFERRLDLLEAEEYEAVAREWRSL
                     SVTLGHRVRITTLAKTFEGEAVDIDEFGALLVRKDTGAMERVLAGDCRHI"
     gene            complement(5708..6130)
                     /locus_tag="VKO45_03910"
     CDS             complement(5708..6130)
                     /locus_tag="VKO45_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2111 domain-containing protein"
                     /protein_id="HMA05052.1"
                     /translation="MRDPVISAGSTAEDWRPLLLALHDLVNRLPVTARSRDHPGIRIE
                     DGRVISMAYTGPVLEEAITRNELRRTTPKSGTYRGVPVTVAPVRNAEGEAVGAIGIVD
                     ITGIFDLATLMEHQSAILKQVCGKDPCPLPSEQVGAKR"
     gene            complement(6162..6740)
                     /locus_tag="VKO45_03915"
     CDS             complement(6162..6740)
                     /locus_tag="VKO45_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05053.1"
                     /translation="MTARTGFLWDSPQIFTRLIEECTGCCELVTPQLLASPFYRGRFS
                     ALVIPTGFGNPLYSRLLPALRASSTRIERFLEAGGRILVFGAADNRPDVYDWLPFRLM
                     YRHDYSVRSIGFTPGSPWVCLIEGYDPGTVPCDGTFPEHDGTVVATAEGGDAVVVERG
                     VGKGLVLATTIHEYPSRSFLASFSCGGEEALL"
     gene            complement(6737..>7483)
                     /locus_tag="VKO45_03920"
     CDS             complement(6737..>7483)
                     /locus_tag="VKO45_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HMA05054.1"
                     /translation="VMIHCGSRGLGHQVCTDHLRELETATKKYGIRLPDRQLACAPIT
                     SPEGKAYFGGMAAAANYAWANRQVITDMTRKVLGKLYGISTEQMPLIYDVCHNVAKME
                     VHRVDGKEVQLCVHRKGATRSFGPGAPDLPAEFRDTGQPVIIPGSMGTPSFLLCGTAG
                     AMEKTFGSTCHGAGRVTSRTQAKKAVRGRDIQDQLAKEGIIVRAPSEASIAEEAPQVY
                     KPSEEVVRVVDAVGISRLVAKLVPLGVIKG"
BASE COUNT         1181 a         2510 c         2625 g         1167 t
ORIGIN      
        1 gtggtcccct cctcggtccc cgggaggacg ggcacccccg ccttcctcat catcctcttc
       61 gagccgatct tgctccccat ggcctcgatg gtcttccagt gggggccgat gaagatgagc
      121 ccctcggact ccacgagttt cgcaaaggcg gagttctcgg cgaggaagcc gtagccgggg
      181 tggatggcgc cggccccggc cttcttcgcc acctccacga ttctttccat gttcaggtaa
      241 gacttcgagg ggtgggcctc cccgacgcag aaggcctcgt cggcgtactt gacgtggagg
      301 gcgttctcgt cggggtccga gtagatggcc acggtctcga tgccgagctc cctgcaggcc
      361 cgcatcaccc ggatggcgat ctccccccgg ttcgcgatga ggatccgctc gaagtcgctc
      421 atcggaccac caggagcacg tcgccgctcg cgaccacgtc cccggtatcc acgaagatct
      481 ccgtgacctt cccgtccctc gggctcgcga tggggttctc catcttcatg gcctcgagga
      541 ccatgagggg atcgcccttc tttaccgcct ccccgacccg gacgaggacc ttcagcacca
      601 tgccctgcat gttcgaccgg atcccgccct cgatctcgcc cctcggggcc ttgccggcgg
      661 aggccgcccc ggcctttgcg acgacggcgg caccggccac cgagacgatc cggaccgagt
      721 acacctcccc gtccacctcc acctccattg ccgagggcac atcgggtacc tgggccgcgg
      781 cggacatctt cggggggagg atctcgggac gcttctcacc cttcaggaac gcaggcgcca
      841 cggcggggta caggatgtac gtgaggacgt cctcctcttt cttcacgagg cccagggaga
      901 ccgcctcctt cttcatgctg tcgtacaccg gttccaggag gtcggcgggc cggacggtca
      961 ccaccttctc gttcccgatg atcttccgcc ggacctcttc gttcacgggc ccggggggct
     1021 tcccgtacag cccccggagg tagtccttca cctccttcgt cacgttcgtg taccgctccc
     1081 cgccgatgag gacgttgaag accgcctggg tccccacgat ctgggaggtg ggggtgacga
     1141 gaggcgggta cccgagatcc tcccggaccc gggggatctc ctggtgcacg tcctcccggc
     1201 ggttcagggc gtcctgctcc ttcagctggg agacgaggtt cgagatcatc ccgccaggga
     1261 gctggtagag gagcacgtcg gagtccaccc gctcggagat ggggtccagg atcccgaggt
     1321 acttctcccg gatggaaagg cagacgttcc tcgcctcccg gaggacgtgg aggtcgatcc
     1381 cggtgtcccg gtcggtcccc tcgacggagg ccaccacgct ctccgtcggg ggctggctcg
     1441 tccccagggc gaagggggac atggccgtgt ccaggatgtc cactcctgcc tcgatggccg
     1501 cctggtagga catgggggcg agcccggcgg tggagtggga gtggaggtcc acggggaggc
     1561 cggtggcctt cttgatgccg gtgacgagct cccgggccgg ctccggcttg atgagcccgg
     1621 ccatgtcctt gatgcagatg gagtcgcagt catggccctg gagctccttc gccatggcga
     1681 tgaatccctt caccgagtgg acgggcgagg tggtgtagca gatggccccc tggaggtggg
     1741 ccccggcctt cttcacctcc ttcatggcct tcttcatgtt ccggacgtcg tttaaggcat
     1801 cgaagacccg aaagatcccg accccgcgcc ggtgggccgc ggcgacaaac ctttccacga
     1861 catcgtcggg gtagtggcgg tagcccacca ggttctgccc ccggaggagc atctggatgg
     1921 gggtcttcgg gagggcctcg gcaaaggccg agagccggtc ccaggggtcg tcgttgcaga
     1981 accggatggc cgtgtcgaag gtcgcccctc cccaggcctc caccgagaag aacccggcct
     2041 tctcgagggc ccgggcgagg gggaggctgt cctcggtccg gaggcgcgtg gcgatgaggg
     2101 actggtgggc gtcccgcagc gtggtgtccg tgatgtgcac tcggtgcata cctgctcttc
     2161 cccggtggcc ggcctgccgg cgtggccgaa ggggtaaaac cttttgaaaa atggatggtg
     2221 caagtataaa aactatcact tcagaggagc agggcgagga gggcgatccc cacggcggag
     2281 agggccttca ccccgtccca cggcccttgg gcgaaccggg ggcagaggct cccgccgtcc
     2341 cggtagcccc ggaccgcgag gatgtccccc tgatcccggg cacgttccag ttccctcttc
     2401 acgaggagcc cgagggccgg gaccagggac atgatggtga ggcggccggc cttttgcctg
     2461 atggcgagga ggaggtcccc gaggtcccgg tccaggacct cgagggatcc cacggccatc
     2521 tcggcgacga ggccgagctc gaacccggtc cggtcgccga gagcccaaac ccccatgtcc
     2581 aggaactccc cccccttccg tgaaccgtag gcccaggtcg cgatgaggag gaccgccgag
     2641 atccggacga ggtaggagag gccgttcccc cccgagaatg cggcgaggac agccgtggca
     2701 aggacaaaga ggaggatacc gaggagggtc cgccaccggc cgagagggag gtgccggggg
     2761 gtggcgacgg cccaccagcc gatggcgagg agggctcccg tcacgcccgc aaaggcggcg
     2821 acggagaggg ccacggtgga gaggaggatc agcctcgggt cctgcacacc gcatccctcg
     2881 catcgccggg ggtgatgttc acgggcaggg ccccgctctc cagggccgca cggaggtatg
     2941 cagggggccg ggaccaccgg gcgatggcct ccggcacccg gcccaggggg aaaaggaccc
     3001 cgtcccggat ctcccagatc tcgtccaccc gggggaggtt ctcgtcctcg tgggtgagga
     3061 ggatggtgat ccccctgcgc cgttccgcga gccggccgga gagcaccgac ttccaggcgc
     3121 agtcgaggag gccgtacggc tcgtcgagga tgaggaggtc ccctgcccgg gccaggatac
     3181 aggagaggac gaggcgcttc agttcaccct ggctgcaccg caggggatcc tccctgcccc
     3241 gcccctcgag gccggcggcc gcaagaaccg gcccgggggc gaccccccat gtaccggcct
     3301 ccgcctccag ggtgctcccg gtcacctggc agcccgggaa gggaagaccc agggtcacgg
     3361 agccgatccc ctccctcgcg atctccccgg tttcggggcg gagggccccg gcgagggcga
     3421 gggcgagggt ggatttcccg ctcccgaccg ggccggtgac caggtgaatc ccctcggaaa
     3481 aggtgcccgc ggccctgagc cggaacgccc cccgccggag agtcacgtcc aggagggaga
     3541 gcctcacagg catccctccc ggggatggga gaagggtgtc cccgagaggg cagcaaggac
     3601 cgcggccggc cttccggagt ggacgaccct gcccccctcc aggaagacga tgtggtccgc
     3661 cgacgaggcg agccccatcc gctgggtggc ctctatgacc tgggagcagg acgaccggcg
     3721 gatcgtatcc atcatcccga tggtgctctc ctggtcgagg ttcgagtctg cctcgtcgag
     3781 gacgaggagg tcagggtccg cggccagggc agcggcgagg gcaaccctgg ccttctcccc
     3841 ggaggagagg gtcccggtct cccgcccgag aagatcggac atgcccagct tctccgcaac
     3901 ctccgtcacc ctctcgtcga cccgggagca gggatccctc cggaaccgga gcggggatgc
     3961 gatctcgtcg tacacccggg tgaagaccat ggtctccccc gggttctccc ccacccaccc
     4021 ggtttccctc cctcggggcg gggttccctc gacgaggatc tccccctccg acgggatgtc
     4081 gatgcccgcg acaagacgga ggagggtggt cttcccgccc ccgttcggcc cgatgaccgc
     4141 cacgatccct gacgggaggg tgagatccgg gatggagagg atccggtggg agagcccggt
     4201 gatccggatc acgggagacg ctcccccagg agaaagaccc ctgcaccctt caggatatcg
     4261 ccgggaagga agggaaccac cccgatgagt accgcggccg ggagggagag ccccgccgag
     4321 aagaggagcc aggcgacccc gcagacatag ataacgaggg ttccggcggc gagcccggct
     4381 ccccggagga tccgggaagg gctcccgtag gcgaagcccg caacggcggc cgccgggatg
     4441 aacccggcga ggaacccgcc ggaggggccg aggaggaccc cgatccccgc gaggccggag
     4501 tggaagaccg gaaggttcgc ggccccgagg aggagataca gggccgccgg tattatcgcc
     4561 cggcgcttca tgaccggcgc ggcgaggagg aggaagaagg tctggagggt gaccgggacc
     4621 gggccgacgg ggatggccac ccaggcaccg gcggcgatga gggcgatgaa ggcagcggtc
     4681 tccgcgagga gccgggtccg ctccgggttt ccgtgcatgg ggtcagatgt ggcggcagtc
     4741 cccggcgagg acccgctcca tcgcccccgt gtccttgcgg acgaggaggg ccccgaactc
     4801 gtcgatgtcc accgcctcgc cctcgaaggt cttcgcgagg gtggtgatcc tgacccggtg
     4861 gcccagggtg acggagaggc tgcgccactc ccgggcgacg gcctcgtact cctctgcctc
     4921 gagaaggtcg aggcgccgtt cgaactcccg gagcaccgat gcaaagaggg cgggccggtc
     4981 cacctccctc ccgagttctg ccgagaggga ggtgaccgtg gcccggagat cggcgggcag
     5041 gtggtcgagg gagatgttcg cgtcgatgcc gagtccgagg agtgcgtact tcacctccga
     5101 gttctcggca gagagttcga ggaggagacc ggccaccttc cggtcaccga ggaggatgtc
     5161 gttcggccac ttgatgaggg ccccgaggtc gaggtccttc ctgagggctc ttgccaccgc
     5221 gatggccgcg gccatggtca ccatgaagac ccggtcgatg ggaatcttcg gcgcgaggat
     5281 ggtggtcgtc catatcccgc ccgggggcga ggcccaggtc cggccgagcc ttccctgccc
     5341 cccggtctgc tcctcggcga tgatcaccat gccgtggagc cgggccgggt cctgctggca
     5401 caggaggtcc cgggcgatcc aggtggtgga ctcggtggcc tccaggtact tcatccggcg
     5461 tccgatgacc ttcgtcccga gatgcctctc tatctcgtag ggaaggagcc gggtggatct
     5521 tccggtgagc tgatacaggt ccttccgggg ttcagcgatg gcgtacccga ggtggcggag
     5581 ccggtccacc gactcgctca cccgggaccg ggggataccc agggaggcgg agagggactc
     5641 ggcgggggcg ctcccccctt cctcgtcgag aaccctgagg accttgaagg cgggatcgtc
     5701 caccggatca cctcttcgcc ccgacctgct ccgagggcag ggggcagggg tccttcccgc
     5761 agacctgttt taggatggcg ctctggtgct ccatgagggt ggcgaggtcg aagatgcccg
     5821 tgatgtccac gatcccgatg gcccccacgg cctccccctc cgcgttcctg acaggggcga
     5881 cggtcaccgg caccccccgg taggtcccgg atttcggggt ggtcctccgg agctcgttcc
     5941 gggtgatggc ctcctcgagg accgggccgg tgtaggccat gctgatgacc ctcccgtcct
     6001 cgatcctgat gcccgggtgg tcccgggacc gggcggtgac cgggagcctg ttgacaagat
     6061 cgtggagggc gaggaggagg ggcctccagt cctctgccgt ggagcctgcc gagatgacgg
     6121 ggtctctcat ggaaccatcc tcactttctc tctcccatcc cctacaataa ggcttcctcc
     6181 ccgccgcagg agaaggaggc aaggaagctc cgggaggggt actcatggat ggtcgtggca
     6241 aggacgaggc ccttccccac tcccctttcc acgaccaccg catccccccc ctcggccgtg
     6301 gcgaccacgg tcccgtcgtg ctcggggaag gtgccgtcgc acgggacggt gccgggatcg
     6361 tacccctcga tgaggcacac ccaggggctc ccgggggtga agccgatgct ccgcaccgag
     6421 tagtcgtgcc ggtacatgag ccggaagggg agccagtcgt agacgtcggg ccggttatcc
     6481 gccgccccga aaacgaggat ccgccctccc gcctccagga accgctcgat ccgcgtggat
     6541 gaggcccgga gggcggggag gagccgggag tagagggggt tcccgaagcc cgtcgggatg
     6601 acgagggccg aaaacctgcc ccggtagaag ggcgaggcga ggagctgggg ggtgacgagc
     6661 tcgcaacaac cggtgcactc ctcgatgagc cgggtgaaga tctgggggga gtcccagagg
     6721 aagccggtcc gggccgtcac cccttgatca ccccgagggg cacgagtttc gcgacgagcc
     6781 gggagatgcc caccgcgtcc accacccgca ccacctcctc ggagggcttg tacacctggg
     6841 gcgcctcctc ggcgatggag gcctccgagg gagcccggac gatgatcccc tccttcgcga
     6901 gctggtcctg aatgtccctc ccccgcaccg ccttctttgc ctgggtccgg gaggtcaccc
     6961 ggccggcacc gtggcaggtg gagccaaagg tcttctccat ggcccccgcc gttccgcaga
     7021 ggaggaagga gggggtcccc atggagcccg ggatgatcac cggctgaccc gtgtcccgga
     7081 attccgcggg aaggtcgggg gcaccgggcc cgaaggaccg ggtcgccccc ttccggtgga
     7141 cgcagagctg gacctccttc ccgtccaccc ggtggacctc catcttcgcc acgttgtggc
     7201 agacatcgta aatgaggggc atctgctcgg tggagatgcc gtagagtttc ccgaggacct
     7261 tccgggtcat gtcggtgatg acctgccggt tcgcccatgc atagttcgcc gccgcggcca
     7321 ttccgccgaa gtatgccttc ccctcggggg aggtaatggg ggcgcaggcg agctgccggt
     7381 cggggagccg gatgccgtac ttcttcgtcg cggtctcgag ttccctgaga tggtcggtgc
     7441 agacctggtg gccgaggccc cgggagccgc agtggatcat cac
//
LOCUS       DATGDI010000104        10169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_155606, whole genome shotgun sequence.
ACCESSION   DATGDI010000104 DATGDI010000000
VERSION     DATGDI010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10169
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155606"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="VKO45_03925"
     CDS             <1..1558
                     /locus_tag="VKO45_03925"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HMA05055.1"
                     /translation="GKGLASASIGALLEARGLSVTLQKFDPYINIDPGTMNPFQHGEV
                     FVTDDGAETDLDLGHYERFIDENLSQLSNVTTGRIYQAVIAKERRGDYLGGTVQIIPH
                     ITDEIKASIGRAAGKPVDHGTAEICLVEVGGTVGDIESMPFLEAVRQMRGELPARDIA
                     LIHVTLVPMDTMGDMKTKPTQHSVKALRELGLAPDIIVARGERPLGEGTKRKISAFCD
                     VKQRAVISAATMPDIYQVPLELEREGLADVLTDYLGLERRDADTSWYTKAMRDYTDRV
                     TVGLVSKYGIEDVYISIKEALKHAGRALATEVQIRWIDAEHYTARDLEEVDGVVVPGG
                     FGSRGIEGKIRAITHARENRVPFLGLCFGFQLAVVEYARSMLGISDAASEEFGSGTFV
                     VAILPEQKGIDQLGGTMHLGNYPVMLKPGTLAGRLYGKTEILERHRHRYEVNPEFIPR
                     LEKAGLVFSGHNGVRMEVCELPGHPFFLATQFHPEFNSRPAHPSPPFVGFVTACRERH
                     GGGPPRERSG"
     gene            1555..2469
                     /gene="guaA"
                     /locus_tag="VKO45_03930"
     CDS             1555..2469
                     /gene="guaA"
                     /locus_tag="VKO45_03930"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HMA05056.1"
                     /translation="MNTGEFIEKAVGEIRAEAGGEKVVMALSGGVDSSVCAALASRAI
                     DDRLVPIYVDTGLMRKGETERIERLFGDLPLHIVRAEDRFFAALRGVTDPEEKRKRIG
                     EQFIRVFEQEARAVNARHLLQGTIYPDRIESQGGIKSHHNVGGMPLSIAFSRIIEPIR
                     DLYKDEVREVAGALGLPPEIQHRMPFPGPGLAVRVLGEVTREKIGVVREANAIAEEEL
                     VERYRPWQCFAALLGKGTGVKGDVRIHGWVIAVRAVNSRDGMTADPLEIPFADLVRIG
                     SRITSEIPSVARVVYDITPKPPATIEYE"
     gene            2489..3610
                     /locus_tag="VKO45_03935"
     CDS             2489..3610
                     /locus_tag="VKO45_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HMA05057.1"
                     /translation="MKEEREREARYYQPVFTRDMKIVRGKGARVWDAEGKEYIDLVAG
                     IAVVSTGHCHPAVVGAICRQSKQLIHCSNLYYVPHQAELAEKLSEITGLSKVFLTNSG
                     TEATEAAIKLARVRTGRKEFVAFTHGFHGRSTGALAVTHKPAIREPFEPLEPKCTFVE
                     YGDLEALRRAVTKDTAGVFVEPIQGEAGVIIPPEGFMAGVREICDETGALLIDDEVQT
                     GMGRTGRWLAIEHEKVKPDIVTLAKGIGSGFPMGALVAREGLEFLKGEHGSTFAGGPL
                     ACAAALATIGVIEGILPTVIPKGERFRHGLSVHNPRVRGLMVGITVGQSCPEVQKRCA
                     ARGVLINCAADGNIRLVPPLVIGDAEIDTAIEVIHEALG"
     gene            3594..4649
                     /gene="hisC"
                     /locus_tag="VKO45_03940"
     CDS             3594..4649
                     /gene="hisC"
                     /locus_tag="VKO45_03940"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HMA05058.1"
                     /translation="MRRSVRPCYEKGGYVYATGAREIALKSGRERVALLASNENPFPP
                     SPPVLDAAMLAMRGVNRYPPEQPVSLLGALRRHHGDYSFAAGAGMDGIIETVIRVLVA
                     PGEKVAVATPTFSFYGVAAKGQGAEIVPVRRERDFSLDPDTFTRAMRDAPLGFICSPN
                     NPTGNAVSPEEIAVILEGIEGVLFLDNAYVDFSDLDYRPLMKKHDNLVIGRTLSKIYA
                     LAGLRIGYAFLPGWLVEPFARAETPFAVSTVAAAAAEAALADREGPAAYLAHVRRWRD
                     RFSREIRGRVFPSEANFVLVDVAPSRSDEMTEALAARGVVVRSCRSFPDLEDHYIRVS
                     VGEDWENERFITEINRP"
     gene            4649..5158
                     /locus_tag="VKO45_03945"
     CDS             4649..5158
                     /locus_tag="VKO45_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HMA05059.1"
                     /translation="MCGITGTPGTGKSSAAEELARRRYPVIHLSDTVGNYAIGRDPER
                     DTTVIDEERWAREFTPGEGFVEGHLAHLLPCDLVVVLRCRPDLLLSRLRERGYREEKC
                     RENAEAEALDVCLIETLERHASDHVLEIDTTPLGPAQVADLIEGFSRGRVPASHGRID
                     WSSYLLEAL"
     gene            5155..5751
                     /locus_tag="VKO45_03950"
     CDS             5155..5751
                     /locus_tag="VKO45_03950"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HMA05060.1"
                     /translation="MTLDQFRPHAAALLRPLTGPCIRAGLTPNSFSAMAFLASVAAGL
                     AFFAGEVPAAILLVAVNALFDSLDGAIAREMKMASTRGDFLDHVLDRYADIFIITGIF
                     AGGAAPWIIGVFGLTGVLMSSYLGTQAQAVGVGRYYGGTLGRADRLVLIVLAGLLTLW
                     LPAGLSGLSFLGWLLAVFGIFGHATAVQRFFYVWGRIK"
     gene            complement(5839..6417)
                     /locus_tag="VKO45_03955"
     CDS             complement(5839..6417)
                     /locus_tag="VKO45_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05061.1"
                     /translation="MPARFLRRRSIIWTVFSTIFGILVFTLLLAALNVIVPPSHEILH
                     GFVVLLNNNLPFFIVIALIFMTGEIFDRFTFPLNLPAPLFHALASVFIVIFFFNVFEF
                     LDMITGAGIYRNLEILRILLSILIFLIVFTGGYFKIFGDLLIRGRERDHQVESPQASS
                     DVKTWNDIGNEFRQLVFDVLQRMREELRGGED"
     gene            complement(6556..6969)
                     /locus_tag="VKO45_03960"
     CDS             complement(6556..6969)
                     /locus_tag="VKO45_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05062.1"
                     /translation="MKPKRSLVKLLLGSIISGIIIKYAAQIAGPDTDPVPLIGQFFRD
                     LIFSQTLTRIPYDIFKVNTLLWLIIFLLIALVPFMFALFAGTWGFLTYVAGFLGGYLL
                     VSGYFFASDFLTLVAGLFCVALGLVTALYGEYIKV"
     gene            7103..7732
                     /locus_tag="VKO45_03965"
     CDS             7103..7732
                     /locus_tag="VKO45_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2150 family protein"
                     /protein_id="HMA05063.1"
                     /translation="MPRKKGTPEEAPLKLFYIFYSRERMENWIRSLEGLSFAGDPSSE
                     DPPEGLLALSRFAEDITIEVLKIMRLFQNGRFTGEEASQRMREVEAIVMSEVRDGEIG
                     EVVESLQLSLLVLFSACRKFMGGKFEKDIKALVKRARALPAAEMEESLELAGQIGAAV
                     IAGTPCCDKYMSDDPGQSTLFDDWLLEIETIRESLKSLKDFDETTGEED"
     gene            7729..8181
                     /locus_tag="VKO45_03970"
     CDS             7729..8181
                     /locus_tag="VKO45_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5814 domain-containing protein"
                     /protein_id="HMA05064.1"
                     /translation="MIAGRARFAGIRKLERTTGLRLPDQVFSGSFLSAISSGVNLRSL
                     DGATRDQIRNILHDFVACGCRDAPDCGCPERRFASRIIGLRESGLDHRQIADALLEEY
                     GISVYPADILSFLEDSVHVLEALADVARLAGEEDLGERCREHRKQIMG"
     gene            complement(8238..9413)
                     /locus_tag="VKO45_03975"
     CDS             complement(8238..9413)
                     /locus_tag="VKO45_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HMA05065.1"
                     /translation="MTLPVILLLVCLSVPASAQVSPAEGSLLWTYTADNSIAGVATTP
                     DASLTVAITTEAGVYAFSRTGGVLWNYPVGKRMTALALAPDGSRIAVGTFDGLTLLAG
                     DGSLLWKKPIGSDVNGVGISSGGEEIAVASSDRLLRLLGPDGSLLWSYTANGAMTSAA
                     ISADGSRVVAGSQDYRVYLLNGAGTLLWSYTTGGKIQALAISADGTSVVAASSDNTVY
                     LLDGTGRLVWRRSFTLAPLSVAVSADGTAIAVGSADYKVSVFDVTGQNTFSAIFDGTV
                     NGVSLSSAGRYLTAGTSRNLVYEYGFLPQTPVVTPSPTPTATSPPVWNTTPVTSLPVT
                     TTLPVTLPPTETTPVAVTQTPGPLPPVGSPLFILVVAGILIGVAALVIAGIHFFRKR"
     gene            9655..10095
                     /locus_tag="VKO45_03980"
     CDS             9655..10095
                     /locus_tag="VKO45_03980"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HMA05066.1"
                     /translation="MKYTSARMGRIFVARVDHGEDFLAGIKNLVVREGISHAVMLFLG
                     ALREGSIVTGPEEPVIPPTPHFVRFGEGWEVVGIASVFPGDEGPVIHVHGSAGRGERA
                     LTGCIRGKAETYLVVEVVVFELAGLTARKNRDPATGLVLPDFDS"
BASE COUNT         1875 a         3145 c         3464 g         1685 t
ORIGIN      
        1 cggcaagggc ctggcctccg cgtcgatcgg cgcgctgctg gaggcgcgcg gcctctcggt
       61 caccctgcag aagttcgacc cctacatcaa catcgacccc ggcaccatga accccttcca
      121 gcacggggag gtcttcgtca ccgacgacgg cgcggagacc gacctggacc tgggccacta
      181 cgagcgcttc atcgacgaga acctttcgca gctctccaac gtcacgaccg gccgcatcta
      241 tcaggcggtg atcgccaagg agcgccgcgg cgactacctc ggcgggacgg tccagatcat
      301 cccccacatc accgacgaga tcaaggcctc catcggtcgt gcagcgggaa agccggtgga
      361 ccacgggacg gcggagatct gcctcgtcga ggtcgggggc accgtcggcg atatcgagag
      421 catgcccttc ctcgaggcgg tccggcagat gcggggcgag cttccggccc gggacattgc
      481 cctcatccat gtcacccttg tccccatgga caccatgggg gacatgaaga cgaagcccac
      541 ccagcactcg gtgaaggccc tgcgggagct cggcctcgcg ccggacatca tcgtggcccg
      601 gggggaacgg cccctcggcg agggaacgaa gaggaagatc tcggccttct gcgacgtgaa
      661 gcaacgggcg gttatttctg cggccacgat gcccgacatc taccaggtgc ctctcgagct
      721 ggagcgggaa ggcctcgccg atgtcctcac ggattacctc gggctcgaga ggagggacgc
      781 cgatacctcg tggtacacga aggccatgcg ggactacacc gacagggtca ccgtcggcct
      841 cgtgagcaag tacggcatcg aggacgtcta catcagtatc aaggaggccc tgaagcatgc
      901 cggccgtgcc ctcgcgaccg aggtgcagat ccggtggatc gacgccgagc actacaccgc
      961 ccgggacctc gaggaggtgg acggggttgt cgtccccggg gggttcggga gccgggggat
     1021 cgagggaaag atccgggcga tcacccatgc ccgggagaac cgggttccct tcctcgggct
     1081 ctgcttcggc ttccagctcg cggtcgtgga gtacgcgagg agcatgctcg ggatctccga
     1141 cgcggcgagc gaggagttcg ggtcggggac cttcgtcgtg gccatcctcc cggagcagaa
     1201 ggggatcgac cagctcgggg gcaccatgca cctcgggaac tacccggtga tgctgaagcc
     1261 ggggaccctc gccggccggc tctacgggaa gaccgagatc ctcgagcggc accggcaccg
     1321 gtacgaggtg aaccccgaat tcatcccccg gctcgagaag gcgggcctcg tcttctccgg
     1381 gcataacggg gtcaggatgg aggtctgcga gctccccggc cacccgttct tcctcgcgac
     1441 ccagttccac cccgagttca attcccggcc ggcccatccc tcccctccct tcgtcgggtt
     1501 cgtcaccgcg tgccgggaga ggcacggggg cgggcctccg cgggagcggt cgggatgaac
     1561 accggggagt tcattgaaaa ggccgtcgga gagatccggg cggaggccgg cggcgagaag
     1621 gtcgtgatgg ccctctcggg cggggtggac agctcggtct gcgccgccct cgcgagccgg
     1681 gccatcgatg accggctcgt tcccatctac gtggacaccg gcctcatgag gaagggggag
     1741 accgagcgga tagagaggct cttcggggat ctccccctcc acatcgtgag ggcagaggac
     1801 cggttcttcg ccgccctgcg gggagtcacg gaccccgagg agaaaaggaa acggatcgga
     1861 gagcagttca tccgggtctt cgagcaggag gcccgggcgg tgaacgcccg gcacctcctc
     1921 caggggacca tctaccccga ccggatcgag agccagggcg ggatcaagag ccaccacaac
     1981 gtcgggggaa tgcccctctc catcgccttc tcccggatca tcgagcccat ccgggacctg
     2041 tacaaggacg aggtgaggga ggtggcgggg gccctcgggc ttcccccgga gatccagcac
     2101 cggatgccct tcccggggcc gggcctggcc gtccgggtcc tcggcgaggt cacacgggag
     2161 aagatcgggg tcgtccggga ggcgaacgcc atcgccgagg aggagctcgt cgagcgttac
     2221 cggccctggc agtgcttcgc ggccctcctc gggaagggga ccggggtgaa gggggacgtc
     2281 cggatccacg gctgggtcat cgccgtgagg gcggtgaact cccgcgacgg catgaccgcc
     2341 gatcccctgg agatcccctt cgccgacctg gtgcgcatcg ggtcccggat cacctccgag
     2401 ataccctccg tcgcacgggt ggtgtacgac atcaccccga aaccccccgc gaccatcgag
     2461 tatgagtgag agagtcaaca tggcaggaat gaaagaggaa agggagcgtg aggcccgcta
     2521 ttaccagccc gtcttcaccc gggacatgaa gatcgtccgg ggcaaggggg cgagggtctg
     2581 ggatgcagaa gggaaggagt acatcgacct ggttgccggg atcgcggtcg tgagcaccgg
     2641 ccactgccac cccgccgtcg tcggggccat ctgccggcag tcgaaacagc tgatccactg
     2701 ctcgaatctc tactacgtcc cccaccaggc agagctcgcc gaaaagctct cagagatcac
     2761 cggcctctcg aaggtcttcc tcacgaactc cgggaccgag gccaccgagg cggccatcaa
     2821 gctcgcccgg gtgaggaccg ggaggaagga gttcgtggcc ttcacccacg ggttccatgg
     2881 ccgctcgacc ggagccctcg ccgttaccca caagccggcc atccgcgagc ccttcgagcc
     2941 cctcgaaccg aagtgcacct tcgtcgaata cggggacctg gaggcactcc ggagggctgt
     3001 cacgaaggac accgccgggg tctttgtcga gcccatccag ggggaggcgg gggtgatcat
     3061 cccgccggag ggcttcatgg ccggcgtcag ggaaatctgt gacgaaacgg gggcactcct
     3121 catcgacgac gaggtccaga ctggcatggg gcggaccggg cggtggcttg ccatcgagca
     3181 cgagaaggta aaacccgaca tcgtgacgct cgcaaagggg atcgggagcg gcttccccat
     3241 gggggcactc gtggcccgcg aagggctcga gttcctgaag ggggagcatg gctccacttt
     3301 cgcgggcggg cccctggcct gtgccgcggc cctcgcgacc atcggcgtca tcgaggggat
     3361 cctccccacg gtgatcccga agggagagcg gttccggcac ggcctctcgg tccacaatcc
     3421 ccgggtccgg gggctcatgg tcgggatcac cgtcgggcag tcctgccccg aggtccagaa
     3481 gcggtgcgcc gcccgggggg tcctcatcaa ctgcgccgcg gacgggaaca tccggctcgt
     3541 cccgcctctc gtcatcggtg atgccgagat cgatacggcc atcgaggtca tccatgaggc
     3601 gctcggttag gccgtgttac gagaaggggg ggtacgtcta tgccacgggt gcccgggaga
     3661 tcgccctgaa atcggggagg gaacgggtcg ccctcctcgc gagtaacgag aatccctttc
     3721 ccccatcccc tccggtcctc gacgcggcca tgctcgccat gaggggcgtg aaccgctacc
     3781 ccccggagca accggtttcc cttctcgggg ccctgcggag gcatcacggc gattattcct
     3841 tcgccgccgg tgccggcatg gacgggatca tcgagacggt catccgcgtc ctggtcgcgc
     3901 cgggagagaa ggtggcggtg gcaaccccga ccttctcctt ctacggcgtt gcggcgaagg
     3961 ggcagggcgc cgagatcgtc ccggtgagga gggaacggga cttttccctc gatccggaca
     4021 cctttacccg ggccatgagg gatgctccgc ttggcttcat ctgcagcccg aacaacccga
     4081 cgggaaacgc ggtatcaccg gaggaaatcg cggtgatcct ggagggaatc gagggggtac
     4141 tcttcctcga taacgcctac gtcgacttct cggacctgga ctaccggcct ctcatgaaaa
     4201 agcacgacaa cctggtcatc ggccggaccc tgtccaagat ctatgccctg gccgggctcc
     4261 ggatcgggta tgccttcctc cccggctggc tcgtggaacc ctttgcgagg gcagagactc
     4321 cctttgccgt gagtaccgtc gctgccgccg ctgcggaggc agccctcgca gaccgggagg
     4381 ggccggccgc gtacctcgcc catgtcagga gatggcggga ccggttttca cgggagatca
     4441 ggggacgggt tttcccctca gaggcgaact tcgtcctcgt ggatgtcgca ccctcccgga
     4501 gcgacgagat gaccgaggcc ctcgcggccc ggggggtggt ggtcaggtcc tgccggagct
     4561 tccctgacct ggaggaccac tacatccggg tgagcgtcgg cgaggactgg gagaacgaac
     4621 ggttcatcac ggaaatcaac cggccatgat gtgcgggatc accgggactc ccggcaccgg
     4681 aaaatcttct gcggcggagg agctcgcccg ccggagatat cccgtgattc atctctccga
     4741 tacggtcggg aactatgcca tcggcaggga cccggagagg gatacgacgg tcatcgacga
     4801 ggagcgctgg gcccgggagt tcactcccgg cgagggattc gtcgaggggc acctggccca
     4861 tctcctcccc tgcgacctcg tcgtggtcct ccgctgcagg ccggacctcc tcctctcccg
     4921 gctgcgggag aggggctacc gggaggagaa gtgccgggaa aacgccgaag ccgaggccct
     4981 ggacgtctgc ctcatcgaga ccctcgagag gcatgcctcc gaccatgtcc ttgagatcga
     5041 taccaccccg ctcgggccgg cacaggtggc agatctcatc gagggtttct cccggggccg
     5101 ggttccggcc tcccatggcc ggatagactg gtcctcgtat ctcctggagg cgttgtgacc
     5161 ctggatcagt tcagacccca tgcagcggct cttctccgtc ccctcaccgg cccctgcatc
     5221 cgtgcgggcc ttaccccgaa cagtttttcc gcgatggcct tcctcgcctc ggtggcagcc
     5281 ggtctcgcct tctttgccgg ggaggtgccg gcggccatcc tcctcgtcgc ggtgaacgcc
     5341 ctcttcgact ccctcgacgg ggccatcgcc cgggagatga agatggcgag cacccggggg
     5401 gacttcctcg accatgtcct cgaccggtac gcggacatct tcatcatcac cggcatcttc
     5461 gccggcgggg cggccccctg gatcatcggg gtcttcggac tcaccggggt gctcatgtcc
     5521 tcctacctgg gcacccaggc ccaggccgtc ggggtaggcc ggtactacgg gggaaccctg
     5581 gggcgggcgg acaggctcgt cctcatcgtc ctcgcggggc tcctcaccct ctggctcccg
     5641 gcgggcctct cggggctctc cttcctcggc tggcttctcg cggtcttcgg gattttcggc
     5701 catgccacgg ccgtccagcg tttcttttac gtctggggaa ggatcaaata gaaaaaccgg
     5761 tttcaatttt ctacctggtc ttcatcttcc cggagctcct cccgcattcg ctggaggaca
     5821 gtccggaaag gctcggccct agtcctcgcc tccccggagc tcctcccgca tccgctggag
     5881 gacatcaaac acgagctgcc tgaactcgtt cccgatgtcg ttccaggtct tgacgtcgga
     5941 ggaggcctgg gggctttcca cctgatggtc ccgttccctc ccccggatga ggaggtcgcc
     6001 gaagatcttg aagtacccgc cggtgaaaac gatgaggaag atcaggatgg agaggaggat
     6061 ccggaggatc tcgagattcc ggtagattcc ggccccggtg atcatgtcca ggaactcaaa
     6121 cacgttgaag aagaagatga caatgaagac gctcgccagg gcatggaaga ggggggccgg
     6181 gagattgagg ggaaaggtga accggtcgaa gatctccccg gtcatgaaaa tgagggcgat
     6241 gacgatgaaa aacgggaggt tgttgttcag gaggacgacg aaaccgtgga ggatctcatg
     6301 ggagggagga acgatgacgt tcagggcggc gaggaggagg gtgaacacga ggatcccgaa
     6361 aatggtcgaa aagaccgtcc agatgatgga tctccttcgt aagaagcggg ccggcacggt
     6421 ttaccccccg gtttggtccg gtttccaaca gtttgccggg gcgggtcata agggtttcca
     6481 gatgagagca cggcattcag gcgggagaaa cggagaagaa gaaaaggagc agaacgggga
     6541 acccgggacc cttccctaga ccttgatgta ttccccgtag agggcggtaa cgaggccgag
     6601 ggcgacacag aagaggcctg cgaccagggt gagaaagtcg gaggcaaaga aatacccgct
     6661 caccagaagg tacccgccga ggaaaccggc aacgtacgtg aggaaccccc aggtgcccgc
     6721 aaagagggcg aacatgaagg gcacgagggc gatgaggagg aagatgatga gccagaggag
     6781 ggtgttaacc ttgaagatat catacggtat gcgggtcagg gtctggctga agatgaggtc
     6841 cctgaagaac tgcccgatca gggggaccgg atcggtatcc ggcccggcaa tctgggcggc
     6901 gtacttgatg atgatccctg agatgatgga gccgaggagg agcttgacga gggatctctt
     6961 tggtttcatc agtgggatga tatagaagtc gtcctatata aatgtatcaa tgatttttgc
     7021 tggacagggc accgagataa tcgcaaaaaa cggggggaaa tgacagaatc ctttaattgt
     7081 ccggagacga agaattgagt gaatgccaag gaagaagggc accccggaag aagcgcccct
     7141 caagctcttc tacatcttct acagcaggga acggatggag aactggatcc ggagcctgga
     7201 gggactgagc ttcgccggtg acccctcttc cgaggatccc cccgagggac tcctcgccct
     7261 ctcccggttc gccgaggaca tcaccatcga ggtgctcaag atcatgaggc tcttccagaa
     7321 cggcaggttc acgggggagg aggcatccca gaggatgcgc gaggtggagg ccatcgtcat
     7381 gtccgaggta cgggatggcg agatcgggga ggtcgtcgag tccctccagc tctccctcct
     7441 cgtcctcttc tcggcctgcc ggaagttcat gggcgggaag ttcgagaagg acataaaagc
     7501 cctcgtgaag cgtgcccggg ccctccccgc ggccgagatg gaggaatccc tggaactggc
     7561 cggtcagatc ggggcggcgg tgatcgcggg caccccctgc tgcgacaagt acatgtccga
     7621 cgatccgggg cagagcaccc tcttcgatga ctggctcctc gagatcgaga ccatccgcga
     7681 atccctgaaa tccctgaagg acttcgacga gaccaccggg gaggaggatt gatcgccggc
     7741 cgggcacggt tcgccggcat ccggaaactc gagaggacca ccggcctccg gctgcccgac
     7801 caggtcttca gcggctcctt tctctcggcc atctcctcgg gggtaaacct gagatccctg
     7861 gacggggcca cgagggatca gatccggaac atcctccatg atttcgtcgc ctgcgggtgc
     7921 cgcgatgccc cggactgcgg gtgtcccgag cggcgcttcg cctcccggat catcgggctc
     7981 cgggaatccg gcctggacca caggcagatc gccgatgctc tccttgagga gtacggcata
     8041 tctgtgtacc ccgccgatat cctctccttc ctcgaggact ccgtccatgt cctcgaggcc
     8101 cttgccgacg tggcacgcct cgcgggcgag gaggacctgg gggagaggtg cagggagcac
     8161 cggaagcaga tcatgggata gccggggcag gggctgcctg cctgccgttc tgttttcccg
     8221 cccgggaacc cgggtggtca cctcttccgg aagaagtgga tcccggcgat gaccagggcc
     8281 gcgaccccga tgaggatccc ggccaccacg aggatgaaca ggggagaccc gaccgggggc
     8341 agggggcccg gggtctgggt gaccgcaacg ggggtcgttt ccgtgggcgg cagggtgacg
     8401 gggagggtcg tggtcaccgg aagggaggtc accggtgtcg tgttccagac cggcggggac
     8461 gtggccgtcg gggtcgggga gggggtgacc accggggtct gggggaggaa cccatattcg
     8521 tagaccaggt tgcgggaggt tccggccgtc aggtaccttc ccgccgatga gagggagacg
     8581 ccgttcacgg tcccgtcgaa gatggcgctg aaggtattct ggccggtcac atcgaagacg
     8641 gagaccttgt agtcggcgga tcccacggcg atggccgttc catcggccga gacggcgacc
     8701 gagaggggcg cgagggtgaa ggaacgcctc cagaccaggc ggcccgtgcc gtcgaggagg
     8761 tagacggtgt tgtccgagga tgccgcaacg acggaggtcc cgtcggcgga gatggcgagg
     8821 gcctggatct tcccgccggt ggtgtagctc cagaggaggg tacccgcccc gttcaggagg
     8881 tagacccggt agtcctggga cccggcgaca acccggcttc catcggccga gatggccgcc
     8941 gaggtcatgg ctccgttcgc ggtgtagctc cagaggaggc tcccgtcagg cccgaggagc
     9001 cggagcagcc ggtccgagga ggcaacggcg atctcctccc cgcccgagga gatccccacg
     9061 ccgttcacat cggatccgat gggtttcttc cagaggaggc ttccgtcgcc tgcgaggaga
     9121 gtgaggccgt cgaaggttcc cacggcgatc cgggacccgt ccggcgcgag ggcgagggcg
     9181 gtcatccgct tgccgacggg atagttccag aggacacccc cggtgcggga aaaggcgtac
     9241 acgcctgcct cagtggtgat agcaacggtg agtgaggcgt ccggggtcgt tgccaccccc
     9301 gcaatgctgt tatccgcggt ataggtccag aggagggacc cctcggccgg ggatacctgg
     9361 gccgaggccg ggactgacag gcatacgaga agcaggatca ccggtaacgt cataccggca
     9421 atccgccccc atagctccgt agggttgttc atggtctgtg gttccccctt cctgatgttt
     9481 tataaagcga gctatccagt tccccgtatt tatggatatc ccagggatgt gccgactgga
     9541 tacagacggg aaaggaggtg aaagggtttt cttccatcgc ccggaatgat taggtgaggg
     9601 atccagccgg agcaccgcgc gtaagggagc gggaccggat gaaggagtga catcatgaag
     9661 tatacgtcgg cccggatggg gcggatcttc gtggcacggg tggatcacgg ggaggatttc
     9721 ctggccggga taaaaaacct cgtcgtgcgt gagggaatct cccacgcggt catgctcttc
     9781 ctgggcgccc tccgggaggg atccatcgtc accggacccg aggagccggt cattccgccc
     9841 acgccccact tcgtccgttt cggggagggc tgggaggtgg tgggaatcgc ctcggtgttt
     9901 ccgggcgatg aggggccggt gatccatgtc catggatcgg cgggacgcgg cgaaagggca
     9961 ctcaccgggt gcattcgggg aaaggcagag acatacctcg tcgtggaggt tgtcgtgttc
    10021 gagctcgccg gccttaccgc caggaaaaac cgcgaccccg ccaccggcct ggtcctcccc
    10081 gacttcgatt cctgagctcc cgacggatcc gctccggggt aatcccggga cccctgataa
    10141 gggggaatcc tgtgcatgga caataatat
//
LOCUS       DATGDI010000105         7526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_39308, whole genome shotgun sequence.
ACCESSION   DATGDI010000105 DATGDI010000000
VERSION     DATGDI010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39308"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="VKO45_03985"
     CDS             <1..1551
                     /locus_tag="VKO45_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HMA05067.1"
                     /translation="AAVKLSDLQKSIGRVVRVHGEISQIKQTSGPTIFTLVDGEGLAN
                     AAAFVEAGVRAYPEVQLGDVVSMMGEVMQRNSQLQVEVSSLAVLTGDEALHVREEIDL
                     TLEARAEPAELPLLVESQVLAALVPALRKVARRVRKAVFMGQPILVRHHADADGISAA
                     VSLEQAVVALIRENGGDFDSDYFLFKRSPSKAPFYEIEDITRDLDFALKDLVRYGQKL
                     PLIVLMDNGSTEEDIPSLQMAAVYDLAMLVVDHHHPDEVVDPFLEGHVNPYHVGGDFG
                     VTSGMLGVEIARLVHPSVEEGIRHIAAVSAVGDRSEAPERGRYLAIAGEAYPEAHCKD
                     IALALDYLQFWLRFNDGRELVKDLLGLNGNPDRHQALVRHLVEQANLAIADQVEASMP
                     HVEERTLVNGVKLFLLDVELHAHKFTFPPPGKTSGEIHDRLIQKNPGAPVVTIGFGPD
                     FAVLRSRGVMMNIPRMVRELRQEITGGGVSGGGHLVVGSVKFVEGMRGQVLEALIGKI
                     AGAPVQPR"
     gene            1693..1944
                     /locus_tag="VKO45_03990"
     CDS             1693..1944
                     /locus_tag="VKO45_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05068.1"
                     /translation="MSTANRIREKHNDTQRKILSYLSSGIRKGKHYFKSKYIAKDLGL
                     SPKEVGTNMGILAEICEELSIIRWSYSNSTTWLVTSRPA"
     gene            1968..2153
                     /locus_tag="VKO45_03995"
     CDS             1968..2153
                     /locus_tag="VKO45_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05069.1"
                     /translation="MARIEITGKIGFVAGIDEKRDCIMSQEHASQDPDALWFNIDLPK
                     GHGLRPGDRVKVTVEKI"
     gene            complement(2298..2924)
                     /locus_tag="VKO45_04000"
     CDS             complement(2298..2924)
                     /locus_tag="VKO45_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05070.1"
                     /translation="MERGDLAAVIIGLSLVIVLMVVLSPPHAPGTEPTTRVPTPVPAT
                     TVPGSLPNPTSGTPPPTPETPTPTPFPAKRILYTADYARFPVRFLPADMALYGFSDIE
                     WPYGSSFVFAYVEENHGGITEPFTVTYPVWKLVTSVSAVKTPEKARFRMILVDRQSGR
                     IIEGVEIRFPGSVTKTVASRGRPLYMVIAAEGVDRFTVSLEAPSEFME"
     gene            3145..3399
                     /locus_tag="VKO45_04005"
     CDS             3145..3399
                     /locus_tag="VKO45_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05071.1"
                     /translation="MNAFSQRTWVPFYLVSFGTLSMAVFRSMDGFMLLGSLVLVTVAL
                     GIIFLAIQAEVTKNLEKKELMEILSKKVLTAGETGDPGAK"
     gene            3406..3990
                     /locus_tag="VKO45_04010"
     CDS             3406..3990
                     /locus_tag="VKO45_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05072.1"
                     /translation="MGNDLYLHHLYQEKEFWNRYDNLKEVLRHLQEQNELLDRIRREE
                     VIPEGPRELAVKALTTDIGQTRRLFSEIFQNFVSGASAGLNRVDLETSLTLLDGNILE
                     VNRCALWVDGEPWEIPADFGRKFAEHILSRGEGTPAEAITGFYESEQHIYDIRLGGSL
                     NRCSLQMMEEIYPAKNLHVRIRLPAQSLVDHHIF"
     gene            4269..4775
                     /locus_tag="VKO45_04015"
     CDS             4269..4775
                     /locus_tag="VKO45_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMA05073.1"
                     /translation="MGVAGQKIKIVVFGAYNAGKSSFIRALDPFSRHIEAENADGSTT
                     VAMDFGRVRIGDCSIFLFGTPGQERFEFARKVLSRGMDGAVVVVDSTRDLDELTLSLF
                     RWLKTRGIPVAVMANKCDCAEALPEQVRELVPFFPVHVISARTGMNVEPSLRAFLAEI
                     LSARVPLP"
     gene            4807..5199
                     /locus_tag="VKO45_04020"
     CDS             4807..5199
                     /locus_tag="VKO45_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05074.1"
                     /translation="MSTGKSVLLLASLLAILSLSTGCLSTATIGPAVYDGSSLRVQVT
                     NSAGAGDAALQVNVFRFQDFRQVEVTREILSVRLERGTGTYSIPLALDDGTFRLYLYV
                     ITNNVRRAAAIQDITVASHGTTPRIGSG"
     gene            5168..6841
                     /gene="ade"
                     /locus_tag="VKO45_04025"
     CDS             5168..6841
                     /gene="ade"
                     /locus_tag="VKO45_04025"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HMA05075.1"
                     /translation="MERLLASALGEEPADAVFHGGTLFNPLDCSWTETSFAVTGGIVV
                     GTGEYRGAETHDLGGARVVPGLVDAHVHPESSLLAPSEFARVVLSHGTTTVIADPHEI
                     ANVAGVPGIEYLMREASRTPLDILLMLPSCVPSAPGDPGGATLSAADLRGFLGREGVI
                     GLGEMMDVPGVLSGDAEVGRKLSLSLIRDGHAPGLSGKPLNAYILSGLQSDHECTTLA
                     EAREKLGKGMFIMIREGSTERNLADLLPLVTPRTADRCSFATDDRHADLLAGAGHVDD
                     CVRKAIALGLDPDLALRMATLSPCGRFGLSDRGAISPGRIADFCILEPGREFRVRETW
                     KRGVRADHAPYRRPEPLRTPFRCRLPDAASLRIDGSGEARVIGLVPGQIATKDLRYDV
                     RGGELPDIGRDILKIVACDRYGRGGLGVGLVHGFGLASGAIASSVSHDAHNILAAGAS
                     DREILRAVREVAARDGGLVVVSKERVTALPLECAGLMSAEPFEEVARQLARLDEEVAS
                     LGGGPHSFMHLSFLALPVIPELRITPRGLFDARAFGPVDLFVADKKRGG"
     gene            complement(6868..>7526)
                     /locus_tag="VKO45_04030"
     CDS             complement(6868..>7526)
                     /locus_tag="VKO45_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05076.1"
                     /translation="VNAINPCALAVLVVILLAIITNNPGRRDQVLLAGLAFSLSIFVF
                     YLAYGLVLVSLLSTVREATSLQPLITRALGLVSIVIGIFHIREYLAPGGPVVGTGIPT
                     GWKPRLGEALNRISSPWGAFVVGGLVTLFLLPCNIGPYIIGCGILAVYGPVVAIPWLL
                     LYNLVFILPMLGITLAVYLGVARISDVKGWREENIGRFHLASGLVILAFGILLVLGLI
                     "
BASE COUNT         1329 a         2475 c         2405 g         1317 t
ORIGIN      
        1 gccgccgtga aactctcgga cctccagaag tccatcggcc gggtcgtccg ggtccacggg
       61 gagatctccc agatcaagca gacgagtggc cccaccatct tcaccctcgt cgacggcgag
      121 gggctcgcca atgccgcggc cttcgtggag gccggggtcc gggcgtaccc cgaggtccag
      181 ctcggggacg tcgtctccat gatgggcgag gtgatgcagc ggaactcgca gctccaggtg
      241 gaggtctcct ccctcgccgt cctcaccggc gacgaggccc tccatgtccg ggaggagatc
      301 gacctcaccc tggaggcccg ggccgagccc gccgagctcc ccctcctcgt ggagagccag
      361 gtcctcgcgg ccctcgtccc tgccctccgg aaggtcgccc gccgggtacg gaaggcggtc
      421 ttcatgggcc agcccatcct cgtccggcac catgccgacg ccgacgggat ctcggcagcc
      481 gtctccctcg agcaggccgt ggtcgccctg atccgggaga acggggggga cttcgactcc
      541 gattacttcc tcttcaagcg ttccccgagc aaggccccct tctacgagat cgaggatatc
      601 acccgggacc tggacttcgc cctgaaggac ctggtccggt acggccagaa gctccccctc
      661 atcgtcctca tggacaacgg atccaccgag gaggacatcc cctccctcca gatggccgcg
      721 gtgtacgacc tcgcgatgct cgtcgtggac caccaccatc ccgacgaggt ggtggacccc
      781 ttcctcgagg gacacgtgaa cccctaccac gtgggagggg acttcggggt aacctcgggg
      841 atgctcgggg tcgagatcgc ccggctcgtg cacccctcgg tggaggaggg catccggcac
      901 atcgccgcgg tctctgccgt cggggaccgg tccgaggccc ccgagcgggg gcggtacctg
      961 gccatcgccg gggaggccta tcccgaagcc cactgcaagg acatcgccct cgccctggac
     1021 tacctccagt tctggctccg gttcaacgac ggccgggagc tcgtgaagga cctcctcggc
     1081 ctgaacggca accccgatcg ccaccaggcc ctggtccgtc acctggtcga gcaggcgaac
     1141 ctggccatcg ccgaccaggt cgaggcctcc atgccccacg tggaggaacg gaccctcgtg
     1201 aacggggtga agctcttcct cctcgacgtg gagctccatg cccacaagtt caccttcccc
     1261 ccgcccggga agacctcggg ggagatccat gaccggctca tccagaagaa cccgggagcc
     1321 cccgtggtca ccatcggctt cgggccggac ttcgcggtgc tccggtcccg gggcgtgatg
     1381 atgaacatcc cgcggatggt gcgggagctc aggcaggaga tcaccggtgg cggggtctcg
     1441 ggagggggtc acctcgtcgt tgggtcggtg aagttcgtgg aggggatgcg cggccaggtc
     1501 ctcgaggccc tcatcgggaa gatcgcgggg gcaccggtcc agccccggtg aggtcagggg
     1561 acggccccgt cggacgacgg ggttcccagc gtcggtacgc taggttccta gcgtcggtac
     1621 gctagggaac cgaaaaggaa cttttttata gtcccccgga gacacattga ggatcaaaca
     1681 gggggcgaat ggatgtcaac cgcgaaccga atccgggaaa aacacaacga cacccagcgc
     1741 aagatcctct cctatctcag ttccggcatc cggaagggga agcattactt caagtccaag
     1801 tacatcgcga aggacctggg cctctccccg aaggaggtgg ggaccaacat ggggatcctc
     1861 gccgagatct gcgaggaact ctccatcatc cggtggagct attcgaacag cacgacctgg
     1921 ctcgtgacct cgcgcccggc ctgacctctt ttccggtttt ccctctcatg gcccgtatcg
     1981 agatcacggg aaagatcggg tttgtcgcag gcatcgacga gaagagggac tgcatcatgt
     2041 cccaggagca tgcctcgcag gaccccgatg cgctgtggtt caatatcgac ctccccaagg
     2101 gccacgggct ccggcccggg gaccgggtaa aggtcaccgt cgagaagatc tgacgggccc
     2161 tttttccgcg atcgcccccg ggccgctttt tccgaaaccg gccccgacgg gcccgttttc
     2221 cgcgatcgaa ccgcctcttc tgaaggtctg tccgcagatc ctcccggtac cgtatcgcga
     2281 atttggggaa ggaaggttca ctccatgaat tccgacggtg cttccaggga gaccgtgaac
     2341 cggtccacgc cctccgccgc gataaccatg tagaggggcc tcccccggga ggcgaccgtc
     2401 tttgtcaccg agccggggaa ccggatctcc accccctcga tgatccggcc gctctgcctg
     2461 tccacgagga tcatcctgaa ccgggccttc tccggggtct tcacggcgga aacgctggtc
     2521 acgagcttcc agacggggta ggtcacggta aagggctcgg tgatcccccc gtggttctcc
     2581 tcgacgtagg cgaaaacgaa ggagctcccg tacggccatt cgatgtccga gaacccgtac
     2641 agggccatgt cggccgggag gaaccggacg gggaaccggg cgtagtcggc tgtgtaaagg
     2701 atgcgtttcg cggggaaggg tgtaggcgtg ggggtttccg gtgtcggcgg gggtgttccc
     2761 gaagtcggat tcgggaggga tccggggacc gttgtggcgg gcactggggt cgggacccgg
     2821 gtggttggtt cggtaccggg tgcatgggga ggagagagga cgaccatgag gacgatgacc
     2881 agggaaaggc cgatgatgac cgcagccaga tctccccttt ccatggagaa accaaggaag
     2941 gctcaccata aatctgtttc ctctgctgcc acccggattc ccgatctgcc cccggaccgc
     3001 cccgggggga aggatgacgg caaataaggg ctgaatactc attgaaccca tcctgatttt
     3061 gtattttgtg aaaaaagggt caatggtaaa ttaaaacaaa atcgattaat atatatatga
     3121 gagtgattaa ctagttgatc aggcatgaac gcattctccc aacgcacctg ggtacccttc
     3181 tacctggtct ccttcgggac cctctccatg gcggtcttcc ggtccatgga cgggttcatg
     3241 ctcctcggaa gcctcgttct cgtcaccgtg gccctcggca ttatcttcct cgccatccag
     3301 gccgaggtca cgaagaacct ggagaagaag gagctcatgg agatcctctc caagaaggtc
     3361 ctcactgcgg gggaaaccgg ggaccccggg gcgaagtgac cggccatggg gaatgacctc
     3421 tacctccacc acctctacca ggagaaggag ttctggaacc ggtacgacaa cctgaaggag
     3481 gtgctcaggc acctccagga gcagaacgag ctcctggaca gaatccggcg ggaggaggtc
     3541 attcccgagg gcccccggga gctcgccgtg aaggccctca cgaccgatat cgggcagacc
     3601 cgccggctct tctcggagat cttccagaac ttcgtatccg gtgcctcggc cggcctcaac
     3661 cgggtcgacc tcgagacatc cctcaccctc ctcgacggga atatcctcga ggtgaaccgg
     3721 tgcgccctct gggtggacgg ggagccctgg gagatcccgg ccgacttcgg gaggaagttt
     3781 gccgagcaca tcctgtcccg gggcgagggt actccggccg aagccatcac ggggttctac
     3841 gagagcgagc agcacatcta cgatatccgg ctcgggggaa gcctgaaccg ctgttctctc
     3901 cagatgatgg aggagatcta tcccgcaaag aacctccatg tcaggatacg cctgccggcc
     3961 cagtccctcg tcgatcacca catcttctga acgggcatcc tttggcatcg gattccatcc
     4021 tgctgccgtc ccggtacccg gttgcccggc atcctgactc attcccgagc gaccgggctt
     4081 ggtagagggg atggcactac aggtatgaat tcatttataa gtctatctat tttgatctca
     4141 tattctttca tttccgctca attttttacc ctgtatttat atccaaaaag ggaactctcc
     4201 ccagataaga ccgttctgtc cggttcaaaa aaggaaatat taaatctggt gccttccaac
     4261 gctatcgtat gggtgtggcc gggcagaaga tcaagatcgt ggtcttcggg gcctacaacg
     4321 cggggaagtc aagcttcatc cgtgcgctcg atcccttctc ccggcacatc gaggccgaga
     4381 acgccgacgg ctccaccacg gtggccatgg acttcggacg ggtccggatc ggggactgca
     4441 gcatcttcct cttcgggacc cccggccagg agcggttcga gtttgcccgg aaggtcctct
     4501 cgagggggat ggacggggcg gtcgtcgtgg tcgactccac ccgggacctg gacgagctca
     4561 ccctctccct tttccggtgg ctgaagaccc gggggatccc cgtcgcggtc atggccaaca
     4621 agtgcgactg tgcggaagcc cttcccgagc aggtgaggga gcttgtgccc tttttcccgg
     4681 tccatgtcat ctccgcccgg accggcatga acgtggagcc ctccctccgt gccttcctcg
     4741 cggagatcct ctctgcccgt gtccccctcc catagctttt tatctccaga acttcacatt
     4801 tcgttaatgt ccaccggaaa atcggtgctc ctcctcgctt cccttctcgc catcctttcc
     4861 ctctcgacgg gctgcctctc cacggccacc atcgggccgg ccgtgtacga cgggagcagc
     4921 ctccgggtcc aggtcacgaa cagcgccggg gccggggacg cggcactcca ggtgaacgtc
     4981 ttccggttcc aggacttccg gcaggtcgag gtgacccggg agatcctttc ggtcaggctc
     5041 gagaggggca cgggcaccta ctccatcccc ctcgccctgg acgacgggac cttccgcctc
     5101 tacctgtatg tcatcacgaa taacgtcagg agggcggcag ccatccagga catcaccgtg
     5161 gcatcccatg gaacgactcc tcgcatcggc tctgggtgag gagccggccg atgccgtctt
     5221 ccacggcggg accctcttca accccctcga ctgctcctgg accgagacct cctttgccgt
     5281 aaccggcgga atcgtcgtcg ggacgggcga ataccggggg gccgaaaccc atgacctcgg
     5341 cggtgcgcgg gtcgtcccgg gcctcgtcga tgcccacgtc catcccgagt cctccctcct
     5401 cgccccctcc gagtttgccc gggtggttct cagccacggg acgacgaccg tcatcgccga
     5461 tccccacgag atcgcgaatg tcgccggggt cccgggtatc gagtacctca tgagggaggc
     5521 ctcccgcacg cccctcgata tcctcctcat gctcccctcc tgtgttccct ccgcaccggg
     5581 cgatcccggg ggcgccaccc tctccgcggc cgatctccgg gggttcctgg gaagggaggg
     5641 ggtcatcggc ctcggggaga tgatggacgt gcccggggtg ctttccggcg atgcggaggt
     5701 gggccggaag ctctccctct ccctgatccg ggacggccat gccccgggcc tctccgggaa
     5761 acccctgaac gcgtacatcc tctcgggcct ccagagcgat cacgagtgca ccacccttgc
     5821 agaggcccgg gagaagctcg ggaaggggat gttcatcatg atccgggagg gctccaccga
     5881 gcggaacctc gcggacctcc tcccgctcgt caccccccgc accgcggacc gctgctcctt
     5941 tgccaccgat gaccggcacg ccgacctcct cgcaggggcg ggccatgtgg acgactgcgt
     6001 ccggaaggcc atcgccctcg gcctcgaccc cgacctcgcc ctccggatgg cgaccctctc
     6061 tccctgcgga cgcttcggcc tctcggaccg gggtgccatc tctcccggca ggatcgccga
     6121 tttctgcatc ctggaaccgg gccgggaatt ccgggtgagg gagacctgga agaggggagt
     6181 ccgagccgac catgccccct accggaggcc ggagccgctc cggacaccgt tccgctgcag
     6241 actgcctgac gccgcctccc tccggatcga cggctcggga gaggcgcggg tgatcggcct
     6301 cgttccgggg cagatcgcga cgaaggattt gcgatacgac gtgcgcggag gggagctacc
     6361 ggatatcggc agggacattc tcaagatcgt ggcctgcgac cggtacggga ggggaggcct
     6421 cggcgtgggc ctcgtccacg gcttcggcct cgcctcgggg gccatcgcct cctcggtctc
     6481 ccacgacgcc cacaacatcc tcgcggccgg ggcctcggac cgggagatcc tccgggcggt
     6541 ccgcgaggtg gccgcccgtg acggcggcct ggtcgtggta tcgaaggaga gggtgaccgc
     6601 ccttcccctg gaatgcgcgg gccttatgtc ggccgaaccc ttcgaggaag tggcgcggca
     6661 gctcgcccgg ctcgatgaag aggtcgcctc cctaggtggg ggcccccact ccttcatgca
     6721 cctctccttc ctcgcgctgc ctgtcatccc cgagctcagg atcacccccc gggggctctt
     6781 cgatgcccgg gccttcggac ccgtggacct gttcgtcgcc gataagaaaa gaggcggctg
     6841 aagggttccc cgacagccgt caccgggtca gatcaggccc aggacgagga ggatcccgaa
     6901 ggcgaggatg acaaggcccg aggcgaggtg gaaccggccg atgttctcct cccgccaccc
     6961 ctttacgtcg ctgatcctcg cgactcccag gtacacggcg agggtgatgc cgagcatggg
     7021 gaggatgaag acgaggttgt agaggaggag ccaggggatg gcgacgaccg gcccgtagac
     7081 cgcgaggatg ccgcagccga tgatgtacgg cccgatgttg cacgggagga ggaagagggt
     7141 gaccaggccc ccgacgacga aggcccccca gggggagctg atccggttga gggcctcccc
     7201 gagcctgggt ttccagccgg tcgggatgcc ggtgccgacc acggggcctc ccggggcgag
     7261 gtactcccga atgtggaaga tcccgatgac gatggagaca agccccaggg cccgggtgat
     7321 gaggggctgg agggaggtcg cctcccggac cgtggagagg agggagacga ggacgagtcc
     7381 gtacgcgagg tagaagacga agatggagag ggagaaggcg aggcccgcga ggaggacctg
     7441 gtcccgcctc cccgggttgt tcgttatgat ggcgaggagg atgaccacga ggacggcgag
     7501 ggcgcaggga ttgatggcgt tcaccg
//
LOCUS       DATGDI010000106         7232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_388403, whole genome shotgun sequence.
ACCESSION   DATGDI010000106 DATGDI010000000
VERSION     DATGDI010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388403"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..586
                     /locus_tag="VKO45_04035"
     CDS             308..586
                     /locus_tag="VKO45_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMA05077.1"
                     /translation="MAFAVHVNLDRCTGCNNCVVACPVNALELHTVDPVTKEKIYCVR
                     DGKAITLDVKSELCAGCGVCVEACPYDVIRLKGRWEAGSKPTVVYVTE"
     gene            595..1788
                     /locus_tag="VKO45_04040"
     CDS             595..1788
                     /locus_tag="VKO45_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA05078.1"
                     /translation="MTLFPKFSKRLEFQKDGKKIIQEQRLLQTTNNLILNVDVCTGCG
                     ICIESCPEEAIKWGPVGAVRRGAVDYAAPVEIDEVKCSYCGVCPVMCPFSALSLTIDG
                     VERYPIIEKEGFPQYDQVSKIDDEKCVKCTVCEDVCPRDAIKRDVPLFEGAAKDGKER
                     QLALKSKTEFKVDMEKCTVCGICGYLCPAITVKKKPFTGESGKVEAVADAKTGLPWDE
                     TKCDACKVCVEACPEECITVERTVESDKIQGKVDIDNDLCCTCTWCSRNCPTEAITVE
                     KLFDGEIEFFPEKCPSGCSTCVEVCPCNAIYLPTPPPAAQMKGSQEAKIAVNKDLCMF
                     CGACVAACPGEDIIVLQRKSIRYKGTETDLFKKIEEKLFTKRTSQVKETVQPGEVQEK
                     MLGKT"
     gene            1798..2388
                     /gene="hdrC"
                     /locus_tag="VKO45_04045"
     CDS             1798..2388
                     /gene="hdrC"
                     /locus_tag="VKO45_04045"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit C"
                     /protein_id="HMA05079.1"
                     /translation="MVKAKGYTDAKLEAKLQDRYYYREYSNPDFTKKVHDLARTVNHM
                     CFQCGTCTGSCVSAPRSSYRIRLFMRRQILGLEDEALTDPDLWLCTTCYSCTDRCPRD
                     IAPTDVIMAMRNFAYQRKIIPVNFLKTVQLIYQSGHGVPNNDVNRAVRVRLGLAKDPP
                     TTHTYTEYMPGIRQIIDHWHVKEIADKIVAEAEKHG"
     gene            2381..3316
                     /gene="hdrB"
                     /locus_tag="VKO45_04050"
     CDS             2381..3316
                     /gene="hdrB"
                     /locus_tag="VKO45_04050"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit B"
                     /protein_id="HMA05080.1"
                     /translation="MAEAKGHDYALFLGCIAPNRYPGCESAGIACSKKLGINLHEMKG
                     ASCCPAPGAFGSIDLNVWYAMAARNVVIAEKMGMDIALECNGCYKSIYEVNEKLKHND
                     DLRDMVNEVLKTVPDPMDPTKKGTMEFKGTIDVFHLVELYSDPKICGPDRIRESVTRP
                     LTGTRIGVHYGCHLIKPKKERHLGDTENPMWIEELVAALGAEPVQYRKKMQCCGAGGG
                     VRGYDLVHALDITNEKLINLHEAKVDAVTECCPFCQLQFDRGQIEIKEKFGKLWGLPV
                     LHFNELLGLAQGMSPTDLALDMHAIPVDSFLKKIL"
     gene            3326..5404
                     /locus_tag="VKO45_04055"
     CDS             3326..5404
                     /locus_tag="VKO45_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HMA05081.1"
                     /translation="MADTKAKAKVKDTKEAKETKAKATPQKARIGVFVCHCGTNIAGV
                     INVKDVIEYAKTLPNVAHAEDYQYVCSTPGQTKIINAIKEKNLTGTVVAACSPRLHEP
                     TFRRATKEGGLNPFRFEMANIREQGTWVHMHDAPGATEKAKDAIRIAVAKASLLQDLT
                     PKSVPVEHAAMVVGAGIAGMQAALDLANAGIKTYLVEKLPTIGGRMSQLDKTFPTLDC
                     SQCILTPKMVDVGRAENIELLTYHEVEAVEGFIGNFDVTLKKKANGVLSMAEAEAKGI
                     AGGGCNGCGDCEPVCPVIKPNPFEMGMKPRKAIYIYHPQVVPLLYTIDFDTCIKCGLC
                     VTACGEKKAIDLEMKDEFRKVKVGTVVLALGYDIFPVEKKREWGYQQFDNVVTSLEFE
                     RLICASGPTAGHLIRPSDGQTPKRVAFVLCAGSRDNTGIGKPYCSRFCCMYSLKHAHQ
                     IIEKIPGVIPYILYMDIRSFGKMYEEFYYRIQDEGTKFIRGRVANILEDKVTKNLHVY
                     AEDTMLNRPIDLEVDMVVLAAAVQPAWDTDRTRKLFGVSCSQDGWLLEAHPKLNPCGT
                     TTAGVFVAGVCQGPKDIPDTVAQAEGAASAASIPIHIGKVELEPYFAQCIEEKCAGCG
                     MCVNLCPYSALSLVDKEGGGKVMQVTEAKCKGCGTCGGFCPGGAIWMQHFATPQVVAQ
                     IDAFLLGGAK"
     gene            5404..5832
                     /locus_tag="VKO45_04060"
     CDS             5404..5832
                     /locus_tag="VKO45_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HMA05082.1"
                     /translation="MTAPEGWKPKILGIVCNWCSYAGADLAGSARAQYPPDIRIVRLM
                     CTGRVDPLFIMKAFMDGADGVLVSGCHFGDCHYLEGNYKSAKRMFLLKSVLKDLGIED
                     KRLRMTFVSASEGAKWAKVVGDVVKTINDLGPSPIKALAK"
     gene            5846..6247
                     /locus_tag="VKO45_04065"
     CDS             5846..6247
                     /locus_tag="VKO45_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HMA05083.1"
                     /translation="MAKIQLNLISGRTIQQGVAIEGGKEKPAYREACGIIELDAEDFK
                     KLGIYRNTNVRVTSPYGTVVVKAVDATQGPHPGLGWIPMGPWANQITNPNTYSTGMPT
                     FKGVPVEVEPAPNELVLLGIELVQKACRAEG"
     gene            6264..>7232
                     /locus_tag="VKO45_04070"
     CDS             6264..>7232
                     /locus_tag="VKO45_04070"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HMA05084.1"
                     /translation="MTKTVTDVICPFCGTLCDDIEVVLSDDGKKILEVMNACAIGNEK
                     FHHSQAADRPTRPRMQQPDGSYKEVSYDEAVEYTAQMFAKSKKPLMYGWSSTNCEAQS
                     VGHEIGELCGAVVDNTATVCHGTTLIAVQDVGIPSCTLGEIKNRADRILYWGCNPAHA
                     HPRHMSRYSIFPRGFFTGKGQASRKVIVVDPRPTDTSNMADYYYQVEQGRDYELLQAF
                     RTALHGHWLPDTVAGIPKEKIYEGAEVMKSGRFGIIFFGMGVTQSLSKNHNIDAAIML
                     TKDLNEFTKFNIMPMRGHYNVTGSGEVLGWQFGFPYAVDLSRGIARY"
BASE COUNT         1604 a         2203 c         2198 g         1227 t
ORIGIN      
        1 tatcatcgat tttcacgcca tttatctcca aaatcattca ccccttttca ccacacattc
       61 accgggtcca ttaaagaatg ttatcatttg atctcttttc cggaaaactc gactggaagc
      121 gacaccatta catatattta ataaggtcca aaaatcaaaa tatatctata aatcttctga
      181 gccttttgcg ggagtccggg cgaaatctga cctctgcgaa ttttcagaag atttaattac
      241 cggcttcgac cacctgatgg tgatcgattt tcaaatcgac aaatcgacag agctggtgcg
      301 tgcaaacatg gcattcgcag tacacgtcaa tctggatcgt tgtaccggct gtaacaactg
      361 tgtggttgca tgccccgtga acgcattgga gctgcacacc gtggatcccg tgacaaagga
      421 aaagatctac tgtgtgaggg atggcaaggc catcaccctg gacgtgaagt ccgagctctg
      481 cgccggatgc ggcgtttgcg tggaagcatg cccgtacgat gttatccggt tgaaaggacg
      541 ctgggaggct ggatccaagc cgaccgttgt gtatgtaacg gagtgatgtg agagatgacc
      601 cttttcccca agttttccaa gaggctcgaa ttccagaagg atggcaagaa gatcatccag
      661 gaacagagac tcctccagac cacgaacaac ctgatcctga acgtggacgt ctgtacgggt
      721 tgtggcatct gcatcgagtc ctgccccgag gaggccatca agtggggccc ggtcggagcg
      781 gtccgccggg gagccgtgga ctatgccgca cccgtcgaga tcgacgaggt gaagtgctcg
      841 tactgcggcg tctgccccgt tatgtgcccc ttctcggccc tctccctcac catcgatggg
      901 gtcgagcggt accccattat cgagaaggag gggtttcccc agtacgacca ggtctcgaag
      961 atcgacgacg agaagtgcgt gaagtgcacc gtctgcgaag acgtctgccc cagggacgcc
     1021 atcaagagag acgtccccct cttcgagggt gcggcgaagg acggcaagga acggcagctg
     1081 gccctcaagt ccaagaccga gttcaaggtc gacatggaga agtgcacggt ctgcggcatc
     1141 tgcggctacc tctgcccggc catcaccgtg aagaaaaagc ccttcaccgg tgagagcggc
     1201 aaggtcgagg ccgtggccga tgcgaagacc ggcctcccct gggacgagac caagtgcgac
     1261 gcctgcaagg tctgcgtcga ggcctgcccc gaggagtgca tcaccgtcga gcggacggtc
     1321 gagagcgaca agatccaggg caaggtcgac atcgataacg acctctgctg cacctgcacc
     1381 tggtgcagcc ggaactgccc gaccgaggcc atcaccgtgg agaagctctt cgacggcgag
     1441 atcgagttct tccccgagaa gtgcccgtcg ggatgctcta cctgcgtcga ggtctgcccc
     1501 tgcaacgcca tctaccttcc gaccccgcca ccggccgccc agatgaaggg atcgcaggag
     1561 gcgaagatcg cggtgaacaa ggacctctgc atgttctgcg gggcctgcgt ggccgcgtgc
     1621 ccgggcgagg acattatcgt gctccagaga aagagcatcc ggtataaggg aacggagacg
     1681 gacctgttca agaagatcga ggagaagctc ttcacgaagc gcacctccca ggtgaaagaa
     1741 accgtccagc ccggagaggt ccaagaaaag atgctcggga agacgtgagg tatcaccatg
     1801 gtcaaggcaa aaggttacac agacgcaaaa ctcgaagcga aactccagga ccggtactac
     1861 taccgggagt acagcaaccc ggacttcacg aagaaggtgc atgacctcgc ccgcacggtg
     1921 aaccacatgt gcttccagtg cgggacctgc accggctcgt gcgtctcggc accacgatcc
     1981 tcataccgca tccggctctt catgaggagg cagatcctcg gcctcgagga tgaggcactc
     2041 accgatccag atctctggct ctgcaccacc tgctacagct gcaccgaccg gtgtccgagg
     2101 gacatcgccc cgacggacgt gatcatggcg atgcggaact tcgcgtacca gcggaagatc
     2161 atcccggtca acttcttgaa gaccgtgcag ctcatctacc agtccggcca cggcgtgccg
     2221 aacaacgatg tgaaccgtgc tgtacgggtc cggctcgggc tcgcaaagga tccccccacc
     2281 acccacacct acactgaata catgcctggt atccggcaga tcatcgacca ctggcatgtg
     2341 aaggaaatag ccgataagat cgtcgcggag gctgagaagc atggctgagg ccaagggaca
     2401 cgactacgcc ctcttcctcg gctgtatcgc cccgaaccgg tacccgggct gtgaatctgc
     2461 ggggatcgcc tgcagcaaga aactcgggat caacctgcac gagatgaagg gtgcgagctg
     2521 ctgcccggca ccgggcgcct tcggctccat cgatctcaat gtctggtatg ccatggcggc
     2581 ccggaacgtc gtcatcgccg agaagatggg aatggacatt gccctcgagt gcaacggctg
     2641 ctacaagtcc atctacgagg tgaacgagaa gctgaagcac aacgacgatc tccgggacat
     2701 ggtgaacgag gtgttgaaga ccgtccccga cccgatggac cccaccaaga aggggaccat
     2761 ggagttcaag gggaccatcg atgtcttcca tctcgttgaa ctctactctg acccgaagat
     2821 ctgcggccct gaccggatcc gggagagcgt aacccggccc cttaccggga cccgcatcgg
     2881 ggtccactac ggatgccacc tcatcaaacc caagaaggag cggcacctcg gcgacaccga
     2941 gaatcccatg tggatcgagg agctcgtggc ggccctgggg gccgagccgg tccagtaccg
     3001 gaagaagatg cagtgctgcg gcgcaggagg cggtgtgcgg ggctatgacc tcgtccatgc
     3061 cctggacatc acgaacgaga agctcatcaa ccttcacgag gcgaaggtag atgcggtaac
     3121 cgaatgctgc ccgttctgcc agctccagtt cgaccgcggg cagatcgaga tcaaggagaa
     3181 gttcggcaag ctgtggggcc tccctgtcct ccacttcaac gaacttctcg gcctggccca
     3241 gggcatgagc cccacggacc tggccctcga catgcacgcc atccctgtcg actccttcct
     3301 gaagaagatc ctgtgaggta cgaagatggc tgacacaaag gcaaaagcaa aggtaaagga
     3361 caccaaggaa gccaaggaaa ccaaggcgaa agcgacaccc cagaaggcac ggatcggcgt
     3421 cttcgtctgt cattgcggta cgaacatcgc gggcgttatc aatgtgaagg acgtcatcga
     3481 gtacgcgaag actcttccga acgtggcgca tgccgaggac taccagtacg tctgctcaac
     3541 accgggccag accaagatca tcaacgccat caaagagaag aacctgaccg gaaccgttgt
     3601 cgcggcctgt tctccccggc tccatgaacc aaccttccgg agagccacca aggagggcgg
     3661 gctcaacccc ttccggttcg agatggccaa catccgtgag cagggcacct gggtgcacat
     3721 gcacgatgcc ccgggagcga ccgaaaaggc caaggatgcc atccggatcg cggttgcgaa
     3781 ggcctcactc ctccaggacc tgactccaaa gagcgtgcct gtcgagcacg cggccatggt
     3841 ggtcggtgca gggatagcag ggatgcaggc tgcccttgac ctggcaaacg ccggcatcaa
     3901 gacctacctt gtggagaagc tcccgaccat cggcggcagg atgtcccagc tggacaagac
     3961 cttcccgacc ctggactgct cccagtgcat cctcaccccc aagatggtgg atgtgggcag
     4021 ggccgagaat atcgagctcc tgacctacca cgaggtcgag gcagttgaag ggttcatcgg
     4081 gaacttcgac gtaaccctca aaaagaaggc caacggcgtc ctttccatgg ccgaggccga
     4141 ggcaaagggg atcgcaggcg gcggctgcaa cggctgcggc gactgcgagc ccgtctgccc
     4201 ggtcatcaaa ccgaacccct tcgagatggg gatgaagccg aggaaggcca tctatatcta
     4261 ccacccgcag gtggtgcctc tcctctacac catcgacttc gacacctgca tcaagtgcgg
     4321 cctctgcgtc accgcatgtg gcgagaagaa ggccatcgat ctcgagatga aggacgagtt
     4381 caggaaggtc aaggtcggga ccgttgtcct ggcacttggc tatgacatct tcccggtgga
     4441 gaagaagcgg gagtggggct accagcagtt cgacaacgtg gtcacgagcc tggagttcga
     4501 gcggctcatc tgtgcctccg gtccgaccgc agggcacctc atccgcccgt ccgacggcca
     4561 gaccccgaag cgggtcgcct tcgtcctctg tgcaggctcc cgggacaaca ccggcatcgg
     4621 gaagccctac tgctcccggt tctgctgcat gtactccctg aagcacgccc accagatcat
     4681 cgagaagata ccgggggtga tcccgtacat cctctacatg gacatccgtt ctttcgggaa
     4741 gatgtacgag gagttctact accggatcca ggacgagggt accaagttca tccggggccg
     4801 ggtggcgaac attctcgagg acaaggtgac gaagaacctc catgtctatg ccgaggatac
     4861 gatgctcaac cggcctatcg atctcgaggt ggacatggtg gtcctcgcgg cagcggtcca
     4921 gccggcgtgg gacaccgacc ggacccggaa gctcttcggg gtctcctgct cccaggacgg
     4981 gtggctcctc gaggcccacc cgaagctgaa cccgtgcggc accactacgg caggggtctt
     5041 cgtggcaggg gtctgccagg gaccgaagga catccccgac accgtggccc aggcggaggg
     5101 tgccgcctcg gcggcctcca tccccatcca catagggaag gtggagctcg agccctactt
     5161 cgcccagtgc atcgaggaga agtgcgcagg atgcggcatg tgcgtgaacc tctgcccgta
     5221 ctcggcactc tccctcgtgg ataaggaggg cggcgggaag gtcatgcagg tgaccgaggc
     5281 caagtgcaag ggctgcggga cctgcggcgg tttctgcccc ggaggcgcca tctggatgca
     5341 gcacttcgcc accccgcagg ttgttgcgca aattgatgcc ttcctcctgg gaggtgcaaa
     5401 gtaatgacag caccggaggg ctggaagcca aagatcctcg ggatcgtctg caactggtgc
     5461 tcctatgcgg gagccgatct cgcggggagc gcccgggccc agtacccgcc cgatatccgg
     5521 atcgtgcgcc tcatgtgcac cgggcgggtc gaccccctct tcatcatgaa ggccttcatg
     5581 gacggggctg acggggtgct tgtgtcggga tgccacttcg gcgactgcca ctaccttgag
     5641 gggaactaca agtctgcaaa gcgtatgttc ctcctaaaga gcgtcttaaa ggatctcggc
     5701 atcgaggaca agcgcctccg gatgaccttc gtctcggcat ccgagggcgc gaaatgggcc
     5761 aaggtggtcg gggacgtggt gaagaccatc aatgacctcg gcccgagccc cataaaagcg
     5821 ctcgcgaagt gacggaggtg tcagtttggc aaagatccag ctgaacctca tcagcgggcg
     5881 caccatccag cagggcgtcg ccatcgaggg gggcaaggag aagcccgcct accgcgaggc
     5941 ctgcgggatc atcgagctcg atgctgagga cttcaagaag ctcgggatat accggaacac
     6001 gaacgtccgg gtcacgagcc cctacgggac cgtcgtggtc aaggcagtgg atgcaaccca
     6061 gggaccccac ccggggctcg ggtggatccc catgggacca tgggcgaacc agataacaaa
     6121 ccccaacacc tactccaccg ggatgccgac cttcaagggg gtccccgtgg aggtagaacc
     6181 cgccccgaac gagctggtgc tcctcgggat cgagctcgtg cagaaggcct gccgggcgga
     6241 aggctgaccg aggtgatgta gacgtgacaa agactgtaac tgacgtgatc tgcccgttct
     6301 gcggcaccct ctgtgacgac atcgaggtcg tgctctccga cgatggcaag aagatcctgg
     6361 aggtcatgaa cgcctgcgcc atcgggaacg agaagttcca ccactcccag gcggccgacc
     6421 ggccgacgag gccccggatg cagcagccgg acgggagcta caaggaggtg agctacgacg
     6481 aggcggtcga gtacaccgcc cagatgttcg cgaagtcaaa gaagcccctc atgtacggct
     6541 ggtcctccac gaactgcgag gcgcagtccg tcggccacga gatcggggag ctctgcgggg
     6601 cggtcgtgga caacaccgcc acggtctgcc acgggacgac cctgatcgcg gtccaggacg
     6661 tgggcatccc gagctgcacc ctcggggaaa tcaagaaccg tgccgaccgg atcctctact
     6721 ggggctgcaa cccggcccac gcccatcccc ggcacatgag ccggtactcc atcttccccc
     6781 gcggcttttt cacggggaag gggcaggcga gccggaaggt catcgtcgta gacccgcgcc
     6841 cgaccgatac ctccaacatg gcggactact actaccaggt cgagcagggc agggactacg
     6901 agctcctcca ggccttcagg acggcgctcc acggccactg gctcccggac acggtggcag
     6961 gcatccccaa ggagaagatc tacgagggcg ccgaggtcat gaagagcggc aggttcggga
     7021 tcatcttctt cgggatgggg gtaacccagt ccctgtcgaa gaaccacaac atcgacgccg
     7081 ccatcatgct cacgaaggac ctgaacgagt tcacgaagtt caacatcatg ccgatgcggg
     7141 gccactacaa cgtgacgggc tccggcgagg tgctgggatg gcagttcggc tttccctacg
     7201 cggttgacct ctcgaggggc atcgcacggt ac
//
LOCUS       DATGDI010000107         3663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_184934, whole genome shotgun sequence.
ACCESSION   DATGDI010000107 DATGDI010000000
VERSION     DATGDI010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184934"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VKO45_04075"
     CDS             complement(<1..747)
                     /locus_tag="VKO45_04075"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HMA05085.1"
                     /translation="MKYIVVTGGVMSGLGKGITTASIGRILKSRGYAVTAVKIDPYLN
                     IDAGTMNPAQHGEVYVLSDGGEVDLDLGNYERFLDIELSSDHNITTGKVYRTVIEKER
                     KGDFLGSTVQIIPHITDEIKASIGRAAGKPVDHGTAEICLVEVGGTVGDIESMPFLEA
                     VRQMRGELPARDIALIHVTLVPMDTMGDMKTKPTQHSVKALRELGLAPDIIVARGERP
                     LGEGTKRKISAFCDVKQRAVISAATMPDIYQ"
     gene            1169..2638
                     /locus_tag="VKO45_04080"
     CDS             1169..2638
                     /locus_tag="VKO45_04080"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMA05086.1"
                     /translation="MLPLAAVLFCVLGSPAMALAGYEARITSGPSQHTWPSLSGDLIA
                     FEDDRNGQVDIYLFNLSSGGEQRLTDEPGEHYWPKIYGDRVVWWENRSGVDNFDVFMY
                     NVTTGVETRITSDPANQENADLYRDRIVWMDRRSGNFDVYLYNTTVGSETRITDDPAD
                     QWEPQVWGDRIVWMDNRSNPGYDPSAGYFDYYTYDLSTRTEQAVTTQGWAWYGGLSGD
                     RLVWSQWRPDSGYPGGYNEQIYLYDFSTGTETRVTSDASNHWYPKVFGDRIVWEDDRN
                     GADSNWDIFLYNATTGTEDQVTSLPAAQMNPVIWGDRIAYEDYRDGNSQIFLYTLISR
                     PIADFAANASTGPAPLAVQFIDRTAGNVTGWLWDFGDGTTSKDRNPVHTYAQPGSYAV
                     SLTATNEAGSDTATRSDAIIATRGILPLPGFVLPPTDPDADGKYEDLTGDGQLRFNDV
                     VAFFKNMKWIAGNEPIPAFDMNGNGRIDFNDVVKLFQEL"
     gene            2645..>3663
                     /locus_tag="VKO45_04085"
     CDS             2645..>3663
                     /locus_tag="VKO45_04085"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HMA05087.1"
                     /translation="MTLRHARFLMIGLVIAALFVSAASAFAVIVHDAEVQAGGTVTIP
                     VEIDEIPVNIWYYQITLAESDPTLAEMVDFAFPSWPTVTDNSTIPADTLTMSGGNLTG
                     PVPAGTQNVSLGSITVRGDNPGSGQITVTVDSMETDNGVLVTPATINGNLVVEPATGS
                     IHVTSNPSGAEIWIDNVDSGQMTEFTIDGITPGDHVVNVTLAGYELAAEQTMTVVAGE
                     TVNADFQLVPLPTTGSIHVTSNPSGAEIWIDNVDSGQTTEFTIDGIAPGDHTVNVTLA
                     GYEMAAEQTVTVVAGETAQADFVLTPVPTTGSIHVTSNPSGAEIWIDNADSGQMTEFT
                     IDGITP"
BASE COUNT          755 a         1166 c         1045 g          697 t
ORIGIN      
        1 ctggtagatg tcgggcatcg tggccgcaga gatgaccgcc cgctgcttta cgtcgcagaa
       61 ggccgagatc ttccgcttcg tcccctcgcc gaggggccgt tccccccggg ccacgatgat
      121 gtccggcgcg aggccgagct cccgcagggc cttcaccgag tgctgggtgg gcttcgtctt
      181 catatccccc atggtgtcca tagggacgag ggtgacgtgg atgagggcaa tgtcccgggc
      241 cggaagctcg ccccgcatct gccggaccgc ctcgaggaag ggcatgctct cgatgtcgcc
      301 gacggtgccc ccgacctcga cgaggcagat ctccgccgtc ccgtggtcca ccggctttcc
      361 cgctgcacga ccgatggagg ccttgatctc gtcggtgatg tgggggataa tctggaccgt
      421 ggaaccgagg aagtcgccct tccgctcctt ctcaatgacc gtgcggtaga ccttgcccgt
      481 ggtgatgttg tgatcggagg agagctcgat gtccaggaac cgttcgtaat ttccgaggtc
      541 gagatccacc tcgcccccgt ccgagagcac gtagacctcg ccatgctggg ccgggttcat
      601 ggtgccggcg tcgatgttca ggtacggatc gatcttcacc gccgtgaccg cgtacccccg
      661 gctcttcagg atcctcccga tggacgcggt cgtgatcccc tttccgaggc cgctcatcac
      721 cccccccgtc accacgatgt atttcacgga gcattccacc cgctggagat caggttggtg
      781 tctcacgtga tatagctacg gaatacgcct gacggtgagt cttccttccc ctccgggatc
      841 cgatccggat tttccaaaaa gggtgagtga gagatagagg aatcaaaata tcccataata
      901 tacaaatctt cctctttgat catgtgtcct ttatttcttt gatcatgaat tgtgcaatta
      961 gattatcgtt aaattacttt ataataagtt tataatgatt ttcacttaaa ggatatattt
     1021 atatacaatt aattgtacga tccggtgaat agccccaaaa ggggtcccgc aggaccgcga
     1081 atatgcccca caatgcccca tgaatccagg ccctgcggta attataaagt gcgtgacgat
     1141 tcggatgacg agcccagcaa ggtaccgtat gctgcccctc gcagctgtcc tgttctgtgt
     1201 cctcgggagc ccggccatgg cacttgccgg gtacgaggcc cgcatcacga gcggaccctc
     1261 ccagcataca tggccctccc tttcggggga cctgatcgcc ttcgaggatg atcggaatgg
     1321 ccaggtcgac atctaccttt ttaacctcag ttccggaggc gaacagcgcc tcacggatga
     1381 gcccggtgag cactactggc cgaagatcta cggagaccgg gtggtctggt gggagaaccg
     1441 ttccggggtc gacaacttcg acgtgttcat gtacaatgtc acgaccggag tcgaaacccg
     1501 gatcacgagc gaccccgcca accaggagaa cgcagacctc taccgggatc ggattgtctg
     1561 gatggacagg cggtcgggca acttcgacgt gtacctgtac aacaccaccg tcggttccga
     1621 gacccggatc acggatgatc ccgccgacca gtgggagccc caggtctggg gagaccggat
     1681 agtctggatg gacaaccggt caaaccccgg ctatgatccc tccgccgggt acttcgatta
     1741 ctacacctat gacctctcga cccggacgga acaggccgtc accacccagg gatgggcctg
     1801 gtacggcggc ctctccgggg atcggctcgt ctggtcgcag tggcggcccg attcgggtta
     1861 ccccggcggc tacaacgagc agatctacct ctatgacttc tccaccggga ccgagacccg
     1921 ggtcacctcc gacgcatcca accactggta cccgaaggtt ttcggggacc gcatcgtctg
     1981 ggaggacgac cggaacgggg ccgactcgaa ctgggacatc ttcctctaca atgccacgac
     2041 cggcaccgag gaccaggtca ccagcctgcc agctgcgcag atgaacccgg tgatctgggg
     2101 agaccgaatc gcctacgagg actatcgcga cggaaattct cagatcttcc tctacaccct
     2161 catctcgaga cccatcgcgg acttcgccgc gaatgccagt accggacccg cgcccctggc
     2221 cgttcagttc atcgacagga cggccgggaa cgtgacgggc tggctctggg acttcggtga
     2281 cgggacgacc agcaaggaca ggaatcccgt ccacacctac gcccagccag gatcctacgc
     2341 cgtgagcctc acggcgacga acgaggcagg gagcgacacc gcgacgcgga gcgacgccat
     2401 catcgccacc cggggcatcc ttccccttcc cgggttcgtc ctgccgccca ccgaccccga
     2461 cgccgatggg aaatacgagg acctgacagg cgacggccag ctgcggttca acgacgtggt
     2521 ggccttcttt aaaaacatga agtggatcgc cgggaacgaa ccgatcccgg cctttgacat
     2581 gaacgggaac gggaggatcg atttcaacga cgtcgtgaag ctgtttcagg agttgtaaac
     2641 gaacatgaca ctcaggcatg cccggttcct catgatcggg ctggtcatcg ctgcactctt
     2701 cgtgtctgca gcatcggcat ttgccgtcat cgtgcatgat gccgaagtcc aggctggtgg
     2761 caccgtcacc atacctgtag agatcgacga gatcccggtg aatatctggt attaccagat
     2821 caccctggcc gaatcggatc ccactctcgc ggagatggtg gatttcgcat tcccctcatg
     2881 gcccacggtt acggataatt ccacgatccc ggcagatacc ctgaccatgt caggtggaaa
     2941 ccttacggga cccgttccag ccgggacgca aaacgtgagc ctcgggagta tcaccgttcg
     3001 cggagacaat ccgggatccg gccaaattac cgtcactgtg gacagcatgg agacggataa
     3061 cggagtcctc gtgaccccgg cgaccattaa cgggaatctc gtggtggaac cagccactgg
     3121 ttctatccac gtcacctcga acccctccgg ggccgagatc tggatcgaca acgtggactc
     3181 cggccagatg accgagttca ccatcgacgg gatcacgccc ggtgaccatg tcgtcaatgt
     3241 caccctcgcc gggtacgagt tggccgccga gcagacgatg accgtcgtcg ccggggagac
     3301 cgtgaacgcc gacttccagc tggtacctct cccgacaacg ggatcgatcc acgtgacctc
     3361 aaacccctcc ggggccgaga tctggatcga caacgtggac tccggccaga cgaccgagtt
     3421 caccatcgac ggaatcgcac ccggtgacca caccgtcaac gtcaccctcg ccgggtacga
     3481 gatggccgcc gaacagacgg tgaccgtcgt cgccggggag accgcccagg ccgacttcgt
     3541 cctgacaccg gttccaacaa ccggatcgat ccacgtgacc tcaaacccct ccggggccga
     3601 gatctggatc gacaacgcgg actccggcca gatgaccgag ttcaccatcg atggtatcac
     3661 gcc
//
LOCUS       DATGDI010000108         7189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_272378, whole genome shotgun sequence.
ACCESSION   DATGDI010000108 DATGDI010000000
VERSION     DATGDI010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272378"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VKO45_04090"
     CDS             <1..217
                     /locus_tag="VKO45_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiS"
                     /protein_id="HMA05088.1"
                     /translation="RMGSEHPMKIQLQDGTVRTYEGDAARASEVLLALDINPAGVIVS
                     RNGRVIPEDVLLGRDDEIRVTRVSHGG"
     gene            159..1070
                     /locus_tag="VKO45_04095"
     CDS             159..1070
                     /locus_tag="VKO45_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HMA05089.1"
                     /translation="MSSSAGTTRSGSPGSPTGGEPTAPGAGGPLPACDLCGGDAVFLD
                     RVRGCHLCADHLLRDVEGRVRRTVRDYRMVRPAERIAVALSGGKDSTVLLHLLRRIVP
                     LGSDITLTAVTVDEGIAGYREATMRVARETAEKLGVEHHVVSFREIAGSSLDDLVAGG
                     GHHPCSLCGIHRREALRRVAGELGATALATGHNLNDEAQTVVMNCLRGDLPGFTRPPV
                     REPAEGRIRRIKPLFRIPEKEVALYAMLAGTFHDLPECPYAGTALRSEARRMLDRLEY
                     LHPGTLGRVVRGQEEVAALWGRRQTEG"
     gene            complement(1110..1874)
                     /gene="fdhD"
                     /locus_tag="VKO45_04100"
     CDS             complement(1110..1874)
                     /gene="fdhD"
                     /locus_tag="VKO45_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HMA05090.1"
                     /translation="MYRDLPCTKIGAKGTREATDPVAEEVPVTVTVNGRQVATAMTSP
                     EGLEEFALGFLFTEGIIKGKGDIDSIQVEKTSVKIITKDLLRVVGPKRTVLSGCGGSS
                     SFLDPSKLPRVTSPLTVEAGEISRLMKELLRSDIHELTGGVHVVGLCRAGKVTEKAFD
                     IGRHNALDRLAGRALIAGTDLGQAYVLSSGRISSEMVRKCLVAGIPLIASRGATTTLA
                     VGLAEKNGLTVIGFVRGGGMNIYTNPQRVTETIPAG"
     gene            complement(1876..3060)
                     /locus_tag="VKO45_04105"
     CDS             complement(1876..3060)
                     /locus_tag="VKO45_04105"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HMA05091.1"
                     /translation="MIPAGIRADFPILSEVCYLDSAATSLSPEPVLSAMTEFEHTFRA
                     NVGRGVHRLAQIASQKYWDAHQKVRRFIGAKEGELVFTRNATEAINMVALGLPWRTGD
                     RVVTTLLEHHSNFLPWMRLRDREGIDLHVITPAAEGGLDLAEFERAITPETRLVAVTH
                     ASNVLGNLVPVPEIAAICRDHGALLLVDGAQSVPHIPVDVEALGCDFLCFSGHKMLGP
                     TGTGALWMRSPGIEPLLLGGGAVDRVTVSGYTLAEGYARYEGGTPNVAGAIGLGRAVE
                     YLESLGMEVVRRHEQALTRRMLEGLGEIGGVKVYGPGPGPERIGVVSFTVGRLHPHEV
                     AHLLDEEADIMVRSGHHCCMPLMTHLGLPDGTVRASLHCYSSVEDVDRLLGAVGAIAR
                     GA"
     gene            complement(3057..3854)
                     /gene="larE"
                     /locus_tag="VKO45_04110"
     CDS             complement(3057..3854)
                     /gene="larE"
                     /locus_tag="VKO45_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HMA05092.1"
                     /translation="MDLDGKGKRLREQIGGRGSMLVAFSGGVDSSLLAAVAREVLGDR
                     CRAVLLESPVVPRSAIRYAEETAAGLGIPLERVLVPLMEEEAFLANPADRCYHCRKVS
                     ARVLKARAAELGYACVADGINSSDLGEHRPGIRAGDEEGIVHPFVEAGITKADIRDIA
                     RRDGYGFWDRPSAACLSSRIPYGERITAGRLGLIERAEDQLRGMGFTQARVRLHGPVA
                     RIEVIPGEMERLWRERGRILEILRPIGFPYVTVDLAGYRSGSMDEVL"
     gene            complement(3938..4267)
                     /locus_tag="VKO45_04115"
     CDS             complement(3938..4267)
                     /locus_tag="VKO45_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMA05093.1"
                     /translation="MRKTPCQQIVWDLLPAIRAALAAELVKNGISQQEVARLLDIAPS
                     AVSQYLSGKRGYRIVFEGPAEKLIGDLAREIKTGGVPDITSRICTICREIRNAQEPCG
                     PERRAGS"
     gene            4457..5203
                     /locus_tag="VKO45_04120"
     CDS             4457..5203
                     /locus_tag="VKO45_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HMA05094.1"
                     /translation="MLSERERERYKRQILLFGEEGQERLKKAGIFIAGAGGLGSPVAI
                     YLAVAGVGTLTIVDSDVVDPSNLNRQILHHDPDIGRKKITSAREKLREINPDITIRAI
                     DAHLDETNAGNLVGDSDGIVDAMDNYPARYTLNRVALDRGIPLFHGAIHGMYGQATTI
                     IPGKTPCLSCIFPKPPPEETLPVVGVTPGLIAMVQATEVLKYLLGTGELLANRLFLWD
                     GMQAQASEICVERNPACEVCGESRRKRRRR"
     gene            5208..5486
                     /locus_tag="VKO45_04125"
     CDS             5208..5486
                     /locus_tag="VKO45_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HMA05095.1"
                     /translation="MKVRIRFFARFRELLGTDIVAEPAPGTSFAGLVREVARRNGEGY
                     RAIFDDSGAFREHVILMRNGKRIEAAEAGETVIDDGDEIAVFPPVSGG"
     gene            5498..5899
                     /locus_tag="VKO45_04130"
     CDS             5498..5899
                     /locus_tag="VKO45_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HMA05096.1"
                     /translation="MISIQTGDVAIGALINRARRPGTGAIVVFDGIVRDDDIRAMELE
                     AYRDVALAELEGIAEDARREFHLLTVDIVHRIGRLKVGDNILVIVVGAGHRKEAYEGS
                     RYIIERIKAGVPIWKKEIRKDGSSWVEGDRG"
     gene            5902..7113
                     /locus_tag="VKO45_04135"
     CDS             5902..7113
                     /locus_tag="VKO45_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-related McrA-glycine thioamidation protein"
                     /protein_id="HMA05097.1"
                     /translation="MVLSPCKKSYREDTHRAVSPEETLARVSEKIPVAGITRVADITN
                     LDRVGIPVFSSIRPTAQEGAVSVYNGKGATPTEARVSAVMEGIERYSAELHHHEISFG
                     TYPGMAARGKAVDPRSLNLPRGADPDGRLPWIEGWDIVADEGVCLPAHAVFHPLPRGC
                     PPLFRTNTNGLASGNTLEEAVFHGLAEIVERDAWSLVEVTRRPGPRITDPPAGLPAEL
                     LGKFADAKVEVTVRDITSDLGIPTCAAVADDALLRDPRLLTIGMGTHTSARIAFLRAL
                     TEVAQSRLTQIHGAREDTTTADLRTRMGYDRVRRMNRYWFEADSEVPFSALPSADNDD
                     FLKDIGAIVAALGKAGMDRVIVADLTREEIGVPVVRVVVPGLEVYAMDNERIGERCRN
                     AERGRLSRPEP"
BASE COUNT         1220 a         2395 c         2467 g         1107 t
ORIGIN      
        1 gagaatggga tctgagcatc ccatgaagat ccagctccag gacgggaccg tgcggaccta
       61 cgagggagat gcggccaggg cctctgaggt gctcctcgcc ctggacatca accccgccgg
      121 ggtgatcgtc tcgaggaacg ggcgggtgat acccgaggat gtcctcctcg gcagggacga
      181 cgagatccgg gtcacccggg tctcccacgg ggggtgagcc gacggccccg ggtgccggcg
      241 ggccgcttcc ggcctgcgac ctctgcggag gggatgcggt cttcctggac cgggtcagag
      301 gctgccatct ctgcgcggat cacctcctcc gcgacgtcga agggcgggtc aggcgcaccg
      361 tccgggatta ccgcatggtc cgtcccgcgg aacgcatcgc ggtggccctc tcgggaggga
      421 aggacagcac cgtcctcctc cacctcctca ggaggatcgt gcccctggga agcgacatca
      481 ccctcacggc cgtcaccgtc gacgagggca tcgcggggta ccgggaggcc acgatgcggg
      541 tggcccggga aacggcggaa aagctcggcg tggaacacca cgtggtctcc ttccgggaga
      601 tcgcgggatc aagcctcgac gacctggtgg cggggggcgg ccaccatccc tgcagcctct
      661 gcgggattca ccggcgggag gccctccgga gggtggccgg ggagctggga gcgaccgccc
      721 tcgccacggg ccacaacctg aatgacgagg cccagacggt ggtgatgaac tgcctcaggg
      781 gggacctccc cgggttcacc cggccgccgg tcagggagcc ggcggagggg aggatccgcc
      841 ggatcaagcc gctcttcagg attccggaga aggaggttgc cctgtacgcg atgctcgccg
      901 gtaccttcca tgatcttccg gaatgcccgt atgcgggcac cgccctgagg agcgaggcga
      961 ggaggatgct cgaccggctg gagtaccttc acccgggtac cctcgggcgg gtcgtgaggg
     1021 ggcaggagga ggtggccgcc ctctggggga gacgccagac cgaaggatag ccgccgctcc
     1081 cggggtgaaa cggaggggct acgaagcatt catccggcgg gtatggtttc cgtgacccgc
     1141 tgcgggttcg tgtagatgtt catcccgcct ccccggacga atccgatcac cgtgaggccg
     1201 tttttttcgg cgaggcccac ggcaagggtc gtggtcgccc cccgggaggc gatgaggggg
     1261 ataccagcaa caaggcactt ccggaccatc tccgaggaga tgcggcccga cgagaggacg
     1321 tacgcctgcc cgaggtcggt gccggcgatg agggcccgac cggcgagccg atcgagggca
     1381 ttatgcctcc cgatgtcaaa ggccttctcc gtcaccttcc cggcgcggca gaggccgacg
     1441 acatgaaccc ctcccgtgag ctcgtgaata tcggacctga ggagctcctt catgagacgg
     1501 gagatctctc ccgcctccac ggtaaggggc gaggtcaccc tcgggagctt cgacgggtcg
     1561 aggaaggagg aggacccccc gcagccggag aggacggtcc tcttcggccc gaccaccctg
     1621 aggaggtcct tcgtaatgat cttcacgctg gtcttctcca cctggatcga gtcgatgtcc
     1681 ccctttccct tgatgattcc ctcggtgaag aggaagccga gggcgaactc ctcgaggccc
     1741 tccggggagg tcatggcggt ggccacctgc ctcccgttga cggtcacggt cacgggcacc
     1801 tcctcggcca cagggtcggt ggcctcccgg gtaccctttg ccccgatctt ggtacagggg
     1861 agatcccggt acatcctatg ccccccgggc gatggccccg accgcgccga ggagccggtc
     1921 cacgtcctct acgctactgt agcagtggag ggaggccctc accgtcccgt cgggaaggcc
     1981 gaggtgcgtc atgaggggca tgcagcagtg gtggccggac cgcaccatga tgtcggcctc
     2041 ctcgtcgagg agatgggcga cttcgtgggg gtgaagcctc cccaccgtga aagagaccac
     2101 cccgatccgc tccggccccg gcccgggtcc gtagactttc accccgccga tttccccgag
     2161 accctcgagc atcctccggg tcagggcctg ctcgtgcctc cgcaccacct ccattcccag
     2221 ggactccagg tactcgacgg cccggccgag gccgatggcg ccggcgacgt tcggggtccc
     2281 cccctcgtac cgggcgtagc cctcggcgag ggtgtacccc gatacggtca cccggtccac
     2341 ggcccctccg ccgaggagga ggggctcgat cccggggctt ctcatccaga gggcgccggt
     2401 tcccgtgggg ccgagcatct tgtggcccga gaagcagagg aagtcgcagc ccagggcctc
     2461 cacgtccacg gggatgtggg gaaccgactg ggcgccgtcc acgaggagga gtgccccgtg
     2521 atcccggcag atcgcggcga tctcggggac cgggaccagg ttccccagca cattcgaggc
     2581 atgggtgacg gcaacgagcc gggtctccgg agtgatcgcc ctctcgaact ccgccaggtc
     2641 gaggcctccc tctgctgccg gggtgatgac atggagatcg atcccctccc ggtcgcgaag
     2701 gcgcatccag gggaggaagt tgctgtggtg ctcgaggagg gtggtgacga cccggtcccc
     2761 cgtcctccag gggagcccca gggccaccat gttgatggcc tctgtcgcgt tccgggtgaa
     2821 gacgagctcc ccctccttcg cgccgatgaa cctccggacc ttctggtgcg catcccagta
     2881 cttctgcgag gcgatctggg caagccggtg cacccccctt cccacgttcg cccggaaggt
     2941 gtgctcgaac tccgtcattg ccgaaaggac cggttcaggc gagaggctcg tcgccgcgct
     3001 gtccaggtag cagacctccg agaggatcgg gaagtccgcc cggatcccgg cgggtatcat
     3061 agcacctcgt ccatggaacc gctccggtag ccggcgaggt ccacggtcac gtacgggaac
     3121 ccgatgggcc ggaggatctc gaggatgcgg ccccgttccc gccagagcct ctccatctcc
     3181 ccggggatga cctcgatccg tgccacgggg ccgtgcaacc gcaccctcgc ctgggtgaaa
     3241 cccatgcccc ggagctggtc ctcggccctc tcgatcagcc cgagcctgcc ggcggttatc
     3301 ctctcgccgt aggggatccg ggacgagagg caggctgcag agggccggtc ccagaacccg
     3361 tacccgtccc ggcgggcgat gtcccggata tcggccttcg tgatccccgc ctcgacgaag
     3421 gggtggacga tcccctcctc gtcccccgcc cggatcccgg gccggtgctc gcccaggtcc
     3481 gaggagttga taccgtcggc gacgcaggcg taccccagct ccgccgcccg tgccttcagg
     3541 acccttgccg agaccttcct gcagtggtag caccggtcgg ccgggtttgc aagaaaggcc
     3601 tcctcctcca tcaagggaac aaggacccgt tcgaggggaa tcccgaggcc ggccgcggtc
     3661 tcctccgcat accggatcgc ggacctcggt accaccggac tctcgaggag aacggcccgg
     3721 catctgtccc cgaggacctc ccgggcgacg gcggcgagga gggaggagtc gacaccgccc
     3781 gagaaggcga cgagcatgga accacggccc ccaatctgct cccgaagtct cttccctttc
     3841 ccgtccagat ccatggttcc cacccggtgc ctccggagcc cgtgttatgc aggaggttca
     3901 cggggagaaa ggataaccct ttcccgctct cccgctttca ggatccggcc cgcctttcgg
     3961 ggccacaggg ttcctgggcg ttcctgatct cccggcagat ggtgcagatc cgggaggtga
     4021 tgtccgggac cccgccggtc ttgatctccc gggcgagatc cccgatgagt ttctcggcgg
     4081 gcccctcgaa cacgatccga tagccccgct tccctgagag gtactgggag acggccgagg
     4141 gggcgatgtc caggagccgg gcgacctcct gttgtgatat gccgtttttc acgagctctg
     4201 cggcaagggc ggcccggatc gccggcagca ggtcccagac gatctgctgg cagggggtct
     4261 tcctcatgca tcatccggat aggaggccgg gggcaataag gggtgccttc gtgatggcga
     4321 agcatagcca tgcggccacc atcagcgggc ttatcatctg ccgagaggga taactcaggt
     4381 gaaaggaccg gagatcggga aggccgggcc ccgcggccga aacgatccat tgccggctcc
     4441 gcatgcgacg gtgtccatgc tatccgagag agaacgagag aggtacaaac ggcagatcct
     4501 tctcttcgga gaggagggac aggagaggct gaagaaggcc gggatcttca tcgcgggagc
     4561 cggggggctc ggctcgcccg tcgcgatcta cctcgccgtg gcgggagtgg ggaccctgac
     4621 cattgtcgac tcagacgtgg tggacccgtc gaacctcaac cggcagattc tccatcatga
     4681 tcccgatatc ggcagaaaga agatcacctc tgccagggag aagctccggg agatcaaccc
     4741 ggatatcacc atcagggcca tcgatgccca cctcgacgag acgaacgcag ggaacctggt
     4801 cggggactct gacgggatcg tcgatgccat ggacaactac ccggcccggt acaccctcaa
     4861 ccgggtcgcc ctcgacaggg ggatccccct tttccacggg gcgatccatg gcatgtacgg
     4921 gcaggcgaca accattattc ccggaaaaac cccatgcctt tcatgcatct tcccgaaacc
     4981 gccccccgag gagaccctcc cggtggtggg ggtgaccccc ggcctcatcg ccatggtgca
     5041 ggcgacggag gtgctgaagt accttctcgg gacaggagaa cttctcgcga accggctctt
     5101 cctctgggac gggatgcagg cccaggcgag cgagatctgc gtggagagga accccgcctg
     5161 cgaggtatgc ggcgagagcc ggcggaagag gcggagaagg tgagaagatg aaggtcagga
     5221 tccggttctt cgcccggttc cgggagctgc tcgggaccga catcgtcgcg gaaccggcac
     5281 ccgggacgag cttcgccggc ctggtccggg aggttgcccg gaggaacggg gagggctacc
     5341 gggccatctt cgatgactcc ggagccttcc gggagcacgt catcctgatg cggaacggga
     5401 agaggatcga ggcggcagag gccggagaga ccgtgattga cgacggggac gagatcgcgg
     5461 tcttcccacc ggtctcgggc ggttagggag gcggcccgtg atctccatcc agaccggtga
     5521 cgtcgccatt ggagccctga taaaccgggc aaggcggccg ggaacgggag ccatcgtggt
     5581 cttcgacgga atcgtccgtg acgatgacat cagggcgatg gaactcgagg cctaccggga
     5641 cgtcgcgctc gcggagctcg agggaatcgc agaggacgcc aggagggagt ttcacctgct
     5701 cacggtggac atcgtgcacc ggatcggccg cctgaaggtc ggggacaaca tactggtcat
     5761 tgtcgtcggg gccggtcacc ggaaggaggc gtacgaagga tcccggtaca tcatcgagcg
     5821 gatcaaggcc ggcgttccca tctggaagaa ggagatccgc aaggacggga gcagctgggt
     5881 ggagggggac cggggttgag gatcgtcctc tcgccctgca aaaaatcgta cagggaggac
     5941 acccaccggg cggtctctcc cgaagagacc ctggcccggg tctcggagaa gatcccggtg
     6001 gccgggatca cccgggtcgc cgatatcacg aacctggacc gggtcgggat cccggtcttc
     6061 tcctccatcc gtcccacggc gcaggagggg gccgtctcgg tgtacaacgg gaagggggca
     6121 acccccaccg aggcccgggt ctccgccgtg atggagggga tcgagcgata ttctgccgaa
     6181 cttcaccacc atgagatcag cttcgggacg taccccggga tggcggcccg gggaaaggcg
     6241 gtggacccca ggtccctgaa cctcccccgg ggggccgacc ccgacggaag actgccctgg
     6301 atcgaaggct gggatatcgt cgccgacgag ggggtgtgcc ttcccgccca cgcggtcttc
     6361 caccccctcc cccggggctg cccgcccctc ttccgcacga acacgaacgg cctcgcctcg
     6421 gggaacaccc tcgaggaggc ggtcttccac ggcctcgccg agatcgtgga gcgggacgcc
     6481 tggtccctcg tcgaggttac gaggagaccc ggcccccgga tcaccgatcc tccggcgggg
     6541 cttcccgccg agctcctcgg gaagttcgcc gacgcgaagg tggaggtgac cgtgcgggac
     6601 atcacgagcg acctcgggat cccgacctgc gccgccgtgg ccgacgatgc cctcctccgg
     6661 gacccccggc tcctcaccat cgggatgggg acccacacct ccgcccggat cgccttcctc
     6721 cgggccctca ccgaggtggc ccagagccgg ctcactcaga tccacggggc ccgggaggac
     6781 accacgacgg cggacctccg cacccggatg gggtacgacc gggtcaggag gatgaaccgg
     6841 tactggttcg aggcagactc ggaggtcccc ttctcggccc tcccctcggc cgacaacgac
     6901 gacttcctga aggacatcgg cgccatcgtc gcagccctgg ggaaggcggg catggaccgg
     6961 gtgatcgtcg ccgacctcac ccgggaggag atcggggtgc ccgtggtccg ggtcgtcgtt
     7021 ccgggcctcg aggtctatgc catggacaac gagcggatcg gggagcggtg caggaatgcg
     7081 gagcgcggtc gtctttctcg gcccgagcct tgagcggggg gcggcccgcc gtatcctcga
     7141 tgccgactac cggcccccgg ccgcccgggg cgacatcacc cgggccgcc
//
LOCUS       DATGDI010000109         4213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_301515, whole genome shotgun sequence.
ACCESSION   DATGDI010000109 DATGDI010000000
VERSION     DATGDI010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301515"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..623)
                     /locus_tag="VKO45_04140"
     CDS             complement(312..623)
                     /locus_tag="VKO45_04140"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HMA05098.1"
                     /translation="MVMSAAMYFISTLRPRRPDFLATMTPDEKAVMGEHASYTKRLFD
                     EGKIILGGAATDGAIGVIVWRLGSAEEARRIYENDPAVKAGIGEAELHPFRVGLLAGK
                     V"
     gene            complement(637..1014)
                     /locus_tag="VKO45_04145"
     CDS             complement(637..1014)
                     /locus_tag="VKO45_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05099.1"
                     /translation="MLRYLPVAILVIALLLASGCTEQAPASVVPSGGLSASVSKVEDH
                     WNLGLGCYWRAYGTVFNSADTTSRGVVVYVQLIDTTSGSIRDAKTLSIGDLAKGGSQS
                     FEVALDGECDHSYRVEVRPTTGA"
     gene            1122..1973
                     /locus_tag="VKO45_04150"
     CDS             1122..1973
                     /locus_tag="VKO45_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HMA05100.1"
                     /translation="MFESIGRSWELVKASWQILSRDRKLLVFPVLSGIVTLAVVASFI
                     LPLILTGAVLGSVAGPAPWYVLLFLFYVVSYFVAIFFNTALVTCVYSLLQGRETSIGD
                     GLSHAVRHAGSILLWAVIAATVGILLRVIGERTGIIGRIAVSLVGGIWSLVTIFVVPV
                     LVLEDKGVVDAMKESVALFRKTWGESVVGSVSIGLIFGAILILGVLPVIASALTGSVA
                     VILLAVALFVLLLVVVGVVASAMQGVFVVALYTYAKTGVVPHPFDRGMVEKAFVPKEP
                     AFGAGNI"
     gene            complement(1987..2262)
                     /locus_tag="VKO45_04155"
     CDS             complement(1987..2262)
                     /locus_tag="VKO45_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HMA05101.1"
                     /translation="MAERRTSFEIYWEILVFCRTPRSITAIINRCDLNSKTGQEYLDF
                     LAGRGYLSVTTEGEKKLFLSTPKAAGYIDTFTRLYQSLFERIPGFRL"
     gene            complement(2265..2912)
                     /locus_tag="VKO45_04160"
     CDS             complement(2265..2912)
                     /locus_tag="VKO45_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05102.1"
                     /translation="MTDTPENRVAGTFAAELRSFFALCLGNLVFGSLAMAFGMQFIVT
                     AVLAMANSGNFGVFPFLQALVGWAGAVIGFRWILSTAGILKGVTGIQREYRTLERSGP
                     ISGDALTGLIIRMTAHYRENWKTIWRMNLISTLGGVIFLALGLANLVQGISALNPAMY
                     LSSALAFLAAGINLVIGLVSLLFSSWFRRFSRAWELRLIEASHSEENLKKTMEQE"
     gene            complement(2989..3735)
                     /locus_tag="VKO45_04165"
     CDS             complement(2989..3735)
                     /locus_tag="VKO45_04165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HMA05103.1"
                     /translation="MFDRLSLSTKILLLLDGILAILTGIYVLSPQYPISPAQPFPAPL
                     PVLAVVSAVGIFILYGALAFIGLKLARRLGLPEIWDAEVSNRDRFLAPAIRGVGIGLF
                     FIAADLLFRNFNSLGAIIHPPFPSSLIASATAGIGEEMIFRLFLISLGTWIVSTKILK
                     GERQDQVFWAVAVFSALLFGFSHLPALMILFGLPSFQQVPPVFLAEVLLLNGVLSLAA
                     ASFFKKSGFLAAVGVHFWADVVWHVLFGLL"
     gene            3923..>4213
                     /locus_tag="VKO45_04170"
     CDS             3923..>4213
                     /locus_tag="VKO45_04170"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HMA05104.1"
                     /translation="MMKQPETAPGKGFRRMITIERTFRANAKDLWDLWTTREGLESWW
                     GPEGFTMKVDRLEVRPGGRFEYEMTATAPEQMEALKRENLPLVSLARSTYTEI"
BASE COUNT          807 a         1284 c         1383 g          739 t
ORIGIN      
        1 agcaacccgc catcaggcgg ggtgcgactg ggaggtttca tcagaaaccg atctagagcg
       61 acccgccatc aggcggggtg cgactgggag gtttcatcag aaaccgatct agagcgatga
      121 agtcgtgagc tcgcctctgg ctggagctta caagccgatt caatctccct ccccagctat
      181 acgccggtgg acgggtcggt ggatattgtg gacgctcccg ggggctttgc ccccgccgct
      241 gaggcaaccc ccattacgat aggcccttca acaggttact ggatttccca ggtggttgga
      301 acgcgtggat atcacacctt gcctgcaagc aatcccaccc ggaacgggtg gagctcggcc
      361 tcgccgatgc ccgccttcac cgccgggtcg ttctcgtaga tccgcctcgc ctcctcggcc
      421 gagccgagcc tccagacgat gaccccgatg gcgccgtcgg tcgccgcacc cccgagaatg
      481 atcttcccct catcgaacag gcgctttgtg taggaggcgt gctcccccat caccgccttc
      541 tcgtccggag tcatggtcgc gaggaaatca gggcgcctcg gacggagcgt ggagatgaag
      601 tacatggctg cactcatgac catcacggat tttttatcaa gcgcctgtcg tcggccggac
      661 ctcgacccgg taggaatggt cgcactcgcc gtccagcgcc acctcgaagg actgggagcc
      721 ccccttcgcg aggtcgccga tggagagggt ctttgcgtcg cggatgctcc cgctcgtggt
      781 gtcgatgagc tggacataga ccaccactcc gcgggaggtg gtgtccgccg agttaaacac
      841 ggtcccgtag gccctccagt aacagccgag cccgaggttc cagtggtcct ccaccttgga
      901 gaccgaggcg gagaggccgc ccgaggggac gaccgaggcg ggggcctgct ccgtgcagcc
      961 gctcgcgagg aggagggcga tcacgaggat cgcgacgggg aggtatcgaa gcatacacca
     1021 cgatgggcgg gggaggatat aggctttttc cggtcgcgaa cagggggctg ggccactggc
     1081 tgtgagaacc ttcatttcaa cccgcatctc agctctcctc catgtttgaa agcatcggca
     1141 ggagctggga gctcgtcaag gcgagctggc agatcctctc gcgggacagg aaactcctcg
     1201 tgttccccgt cctctcgggg atcgtcaccc tcgccgtggt cgcgagcttc atcctccccc
     1261 tcatcctcac gggggcggtc ctcggcagcg tggcgggacc ggcaccctgg tacgtcctcc
     1321 tcttcctctt ctacgtcgtg agctatttcg tggccatctt cttcaacacg gccctcgtca
     1381 cctgtgtgta ctccctcctc cagggccggg agacgagcat cggggacggg ctctcccacg
     1441 ctgtccggca cgcgggttcc atcctcctct gggccgttat cgccgcgacg gtggggatcc
     1501 tccttcgggt catcggggag cggaccggga tcatcggcag gatcgcggtt tccctcgttg
     1561 gcgggatctg gagcctcgtc accatcttcg tggtcccggt cctcgtgctc gaggacaagg
     1621 gggtggtcga tgccatgaag gagtccgtcg ccctcttccg gaagacctgg ggggagagcg
     1681 tggtcgggtc agtctccatc gggcttatct tcggggccat cctcatcctc ggagttctcc
     1741 cggtgatcgc ctccgccctc acgggcagcg tggcggtgat cctcctcgcg gtcgccctct
     1801 tcgtcctcct cctcgtcgtg gtcggggtgg tcgcgtcggc gatgcagggg gtctttgtcg
     1861 tggccctcta tacatacgca aaaacaggtg tggttcccca ccccttcgac cgcgggatgg
     1921 tggagaaggc ctttgtcccg aaggagccgg ccttcggggc ggggaacatc tgaatccctt
     1981 ttccctctac agccgaaagc ccgggatcct ctcgaaaaga gactggtaga gccgggtgaa
     2041 ggtatcgatg tacccggctg ccttcggagt ggaaaggaac aatttcttct ccccctccgt
     2101 cgtgaccgag aggtaccccc tgcccgcaag gaaatccagg tactcctgcc cggtctttga
     2161 gttcaggtcg caccggttga tgatggcggt gatggaccgg ggggtccggc agaagaccag
     2221 gatctcccag tagatctcga aggaggtcct tctttcggcc atgctcactc ctgctccatc
     2281 gtcttcttca ggttctcctc cgaatgagag gcctcgatga gccggagctc ccatgcccgg
     2341 gagaaccggc ggaaccagga ggagaagagg aggctcacga ggccgatgac gaggttgatc
     2401 cccgccgcga ggaaggcgag ggcggaggag agatacatgg ccgggttcag ggcagaaatt
     2461 ccctggacga ggtttgcgag gccgagggcg aggaagatca ccccgcccag ggtcgagatg
     2521 aggttcatcc tccagatggt cttccagttc tcccggtagt gggccgtcat ccggatgatc
     2581 aggccggtga gggcatcacc ggagatgggc ccggaccgtt ccagtgtccg gtattccctc
     2641 tggatcccgg tcaccccctt caggatcccc gcggtcgaga ggatccaccg gaacccgatc
     2701 accgccccgg cccatccgac cagcgcctgg aggaacggga agaccccgaa gttcccggag
     2761 ttcgccatgg cgaggaccgc cgtcacgatg aactgcatcc cgaaggccat ggcgagggaa
     2821 ccaaagacca ggttcccgag gcacagggcg aagaaggacc tgagctcggc ggcgaaggtg
     2881 ccggcaacgc ggttctctgg ggtatcggtc atggttgttt tatcaccgga atatcatccg
     2941 ctggccgctt ataaagggag gcatgaacct gagatacgta cctgttgttc agagcagtcc
     3001 aaacaggaca tgccacacga catcggccca gaagtggacc ccgacggccg cgaggaaccc
     3061 ggatttcttg aagaaggacg cggcggccag ggagaggacc ccgttcagga gcaggacctc
     3121 ggcgaggaag accggcggga cctgctggaa agagggaagg ccgaagagga tcatgagcgc
     3181 cggcaggtgg ctgaaaccga agaggagcgc agagaatacg gccacggccc agaagacctg
     3241 gtcctgccgc tcccccttca ggatcttcgt ggagacgatc cacgtcccga gggagatgag
     3301 gaagaggcgg aagatcatct cctccccgat ccccgcagtt gccgaggcga tgagggatga
     3361 ggggaacggc ggatgaatga tcgccccgag ggaattgaaa ttccggaaga ggaggtctgc
     3421 ggcgatgaag aacaggccga tgcctacgcc cctgatcgcc ggagcgagga acctgtcccg
     3481 gttcgacacc tccgcatccc agatctcggg gaggccgagc ctccgggcga gtttcagccc
     3541 gatgaaggcc agggccccgt agagaatgaa gatcccgacg gcactcacca ccgcgaggac
     3601 cggtaagggc gccggaaagg gctgtgccgg ggaaatgggg tactgggggg agaggacgta
     3661 gatccccgtg agaatcgcga ggataccgtc gagcaggagg aggatctttg tcgagagcga
     3721 gaggcgatcg aacatccgac acaggtttgg agggatttcg tattaaggga accggaacca
     3781 gggtcccggg gcggtgcccg gggccggatc caggtccggc cgctcggcac cgggttctct
     3841 tctcacgtcc tgcaacgtgg acaaaccttc ttatccatga cccgctcatg cacccgcgcc
     3901 tgaaaagcag gaggagcaac cgatgatgaa acaacccgag accgcgccgg ggaagggctt
     3961 ccggaggatg atcaccatcg aacggacctt ccgggcgaac gcgaaggatc tctgggatct
     4021 ctggaccacg agggagggcc tcgagtcctg gtggggtccc gaggggttca ccatgaaggt
     4081 cgacaggctc gaggtccgcc cgggcggcag gttcgagtac gagatgaccg cgaccgctcc
     4141 ggagcagatg gaggcattga aaagagagaa ccttcccctc gtgagcctgg ccaggagcac
     4201 ctacaccgag atc
//
LOCUS       DATGDI010000110         9565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_243508, whole genome shotgun sequence.
ACCESSION   DATGDI010000110 DATGDI010000000
VERSION     DATGDI010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9565
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243508"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(654..1541)
                     /locus_tag="VKO45_04175"
     CDS             complement(654..1541)
                     /locus_tag="VKO45_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMA05105.1"
                     /translation="MAKANILIVEDDFVIAKVLAESLQELGYQVAGIVSTGEEAVERA
                     AKVHPDLVLMDIRLKGEMDGIDAGEQISGDLHIPLVYLTAYSDERTVERAKITEPYGY
                     LIKPFTDTELKTTLEMAIYKHRREKREREKGEWFLETLMAISDGIITTNTEGQVTFMN
                     PSAELMTGWQSPEAYLRPLPEVFQLEHEGSGAAVENLARRAAREENLVTGDGDEVLVA
                     KNGTKWPVEGRFSPIRDPRGKAIGAVAIFRPRGGKDERAGEELQTIRNIEGVFRSMAP
                     DLLKQIDLDYLSGTPPKKV"
     gene            complement(1556..3079)
                     /locus_tag="VKO45_04180"
     CDS             complement(1556..3079)
                     /locus_tag="VKO45_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HMA05106.1"
                     /translation="MTRSTPRERETAPGDDAITGGLLDLLPDGLLLIDGSGKIVHLNS
                     AMEQVSGMRAEESRGMTARDFIAKGIARYLAGDEGGIRRLTGILSTGGEGKGIEFALS
                     GPKGVRWFSYITLPLRDAPYQGGRLDTYREVTGNRRAGQARSDQEDRLLAIFLRAMKM
                     SLSRDRELTERERRLSAIFEAAQNVAFVTSDLRGKESRILDFSPGAEGIFMYWKGDIV
                     GEKLALLYPPEELELLAEIHRKMKRKQVEYSGEITLVRKNGEQFPALLNMYPLRDEAG
                     KVKAAVSVCIDITILKRAEAQIRATLLEKENLLREIHHRVKNNMQVVSALLILQSKKT
                     KDPRILEMFRESEHRIKTMALIHEKLYQSRSFGTIDLAEYLTRLLQYLFRSYEERSKG
                     VSLQMDVRDISLDLDTALPVSLIMNELVSNSLKYAFPGEKGGKICIRVAREEGKGFVM
                     EIRDDGIGLPPGFNLEETGTLGLHLVHGLAVSQLGGSIELGKGKGTSFIIRFPDMKK"
     gene            3531..3821
                     /locus_tag="VKO45_04185"
     CDS             3531..3821
                     /locus_tag="VKO45_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05107.1"
                     /translation="MDEILDRLLKVRDISRDKVRDLGFDIRSVDLDNLPVELDKEKVY
                     WISFYKNLFNFISIPLFVNGVIDQQEIASIRFDEHCKHDIAEILTSIDVEAS"
     gene            3979..4707
                     /locus_tag="VKO45_04190"
     CDS             3979..4707
                     /locus_tag="VKO45_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA05108.1"
                     /translation="MLDVGKVTKLIGGMTILSEVSLSVQKGEIVGVVGPNGAGKTTLF
                     NVITGISRPNRGSISFEGEEITGLTPEQICRKGVAKTFQIAHCFPNLTARESVMVGAI
                     FGTDGVPSMGEAGEESEKILGYVGFPGEKADTLVRNLNVVELKKVQLARALASKPRLL
                     LFDELMTGLNTAESKDSVELVKKIRDQGISILMIEHVMSVIMGVSDRVVVLDHGEKIA
                     DGHPYEVINDQRVIDTYLGDVDAF"
     gene            4721..5416
                     /locus_tag="VKO45_04195"
     CDS             4721..5416
                     /locus_tag="VKO45_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA05109.1"
                     /translation="MLEIEKLNIFHGNLQVVWDLSLSVNSGELVTLIGPNGAGKTSTV
                     ETIIGLNPRAKGSIRFMGEEILGMPPHRIFGKGIALVPEQREIFPKMRVSENLQLGAH
                     GRAGWEKKLEHVFSLFPVLSERERQVAGTLSGGEQQMLAIGRALMSEPRMLVLDEPST
                     GLSPILVGQVFRSLEQLGREGLTVLLLEQNVRHALELCTRGYVLENGRIVLQGKTRDL
                     LHDPHIERAYLGI"
     gene            complement(5650..6642)
                     /locus_tag="VKO45_04200"
     CDS             complement(5650..6642)
                     /locus_tag="VKO45_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HMA05110.1"
                     /translation="MRVERGRSSWYLTGIALAIAVLLPLIAGMSFYVLNVVIIMLTFV
                     LFSSAWNFLAYSGQASLGHAAFFGIGGYVSTLIARATGFSPFGTILMGGAVAAVIGLL
                     LGLTCVRLKEWFLAMVTFGFAVILQAVIASPLAATTGGWDGIGSPRLLPAEIQGYLAY
                     EYYAILAITVISILCIHLILRSRMGLAFAAIRENELEARASGVNPVRYKLLAFTVSSF
                     IAGVAGALQVHHISFISPEIFHAETSFWPVIYSIFGGLGTISGPVIGTVVLTVIWEGL
                     KGLGLTFERFVVIGVLLIVVVIFLPRGLVSLPEEFRRWRESRKANPGPERGSDG"
     gene            complement(6642..7523)
                     /locus_tag="VKO45_04205"
     CDS             complement(6642..7523)
                     /locus_tag="VKO45_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HMA05111.1"
                     /translation="MALDLGILIQVLFWGLYAGCIYILLATGLNLIFGVMKIVNFAHG
                     EVLMLGAYITASVFLLSGLNPYVIILLTMLALGLIGILVERLCFRPILGTGKLNEIFL
                     SLGLIYIFQNGVALIWGDDRQVLNSPYQNISIPIGAIRLPLDYIIIIAITALILCGLY
                     FLLRRTELGMAIRATSQNRKGAMIVGIDVERMDMISFGIGSGLAAAAGTLWVVSGQVF
                     NPYIGSIPAIKAFAIIILGGLGSIPGAIVGGLLYGIAENGAAYFLGGIWKDSISFLIL
                     IVVLIFRPTGLFGEAGE"
     gene            complement(7582..8898)
                     /locus_tag="VKO45_04210"
     CDS             complement(7582..8898)
                     /locus_tag="VKO45_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA05112.1"
                     /translation="MKKTTVSILLAALVLGSVLLAGCAQQQAQPQDIKIGAVVSLTGA
                     GSNVGKHMKQSAELAVSEINAQGGVFVKAYNKKLPITLIVADDETKPDSAVKAVTKLA
                     SEDKVDLLVGGYSSAVTIATQGVVAENKIPYVVTGASSPDVTRKAGVDTSYMFHFCPT
                     TAMYGQYTTLFMDQVMRPAINEKFEFNSSRPMRLAILYQDSAYGKGVLAGVTNTITAN
                     NLNIQIVSNQSFKLGETDFRTALTAIKAARPDAIYAAAFPAEQTQIDVQARRDVNLNT
                     IILAVETNDNPDYYKGIGHFGEYSIIESRFSPYATPASAIATADVKFKTDYQAKYGTF
                     PDMMGTSTYEGVYIEAQAIQNAGTLDKAAVRDALASLSMPEIVEAIKDRTITFSKDYR
                     EVQFSLFMEQLIWNAQAGEVRPKIVWPDELKEQEFVLPDWYQAGSG"
     gene            9072..9194
                     /locus_tag="VKO45_04215"
     CDS             9072..9194
                     /locus_tag="VKO45_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05113.1"
                     /translation="MRIGEHFQYSPWHPCTMDGFMYGMMYGNPYGWAFPWMIVA"
BASE COUNT         1844 a         2861 c         2958 g         1902 t
ORIGIN      
        1 gtccaggggg gtttggggcc cctcaggact tgagcgacgg gtccgaagga cccggagctg
       61 tccaggaggg tttggggcct ctctggactt gagcggaggg tccgagggag cggaggatcc
      121 gatggagcga gggtccgaag gaacgatttg gacaggaaag gagatgcggg gttcacccgg
      181 gggaagaaat cccccgggca tccccgggtg gaagacccgc ctattcagac gggaaaaaga
      241 gggtagagtt cctcggggta ccgggcggct gatagcggga gtgctcatca cgggtggtgt
      301 ggggattgag agttccctcc cggccctttg gatctcccca gattttcatg cacatgaggt
      361 gttttctcct ttcgtgtggt ctctctcttg taaccagagg gcttcgagcc ccaactggtt
      421 cacacataac aataattact aatagtatat atacatttgc acataatcat cttcatgaaa
      481 atcatcaatt atgtgaaatt agcgataatc gcaggtaata tccccaaaaa aaggattccg
      541 ggaggggtat caggcggttg aaaaacctcc aaatcggcga ggtggccggc ggtggatccc
      601 gttcattcct tccgccttcc ctcgtcccgg tctccgtccc cgtggcaagg ccttcagacc
      661 ttcttcgggg gcgtcccgga caggtaatcg aggtcgatct gcttgaggag atccggggcc
      721 atggaccgga agaccccctc gatgttcctg atggtctgga gttcctcgcc cgcccgctcg
      781 tccttcccgc cccggggccg gaagatggcc acggcgccga tggccttccc ccggggatcc
      841 cggatggggg aaaaccggcc ctcgacgggc cacttcgtcc cgttcttcgc cacgagaacc
      901 tcgtccccgt ccccggtcac caggttctcc tcccgggcgg ctcgccgtgc caggttctcc
      961 accgccgccc cgctcccctc gtgctctagc tggaagacct cgggcagggg ccgcaggtac
     1021 gcctccgggg actgccaccc ggtcatgagt tccgccgacg ggttcatgaa ggtgacctgc
     1081 ccctcggtgt tcgtggtgat gatgccgtcg ctgatggcca tgagcgtctc gaggaaccac
     1141 tcccccttct cccgctcccg cttctccctc cggtgcttgt agatggccat ctcgagggtg
     1201 gtcttgagtt ccgtgtcggt gaagggcttg atcaggtacc cgtacggctc ggtgatcttg
     1261 gcccgctcca ccgtccgctc atcggaatac gcggtgaggt acacgagggg gatgtggaga
     1321 tcgcccgaga tctgctcgcc tgcatcgatc ccgtccatct cccccttgag ccggatgtcc
     1381 atgaggacga ggtcggggtg gaccttggcc gcccgctcca ccgcctcctc gccggtcgag
     1441 acgatacccg ccacctggta cccgagctcc tggaggctct ctgcgaggac ctttgcgatg
     1501 acaaagtcgt cctccacaat caggatattg gctttggcca cgtggaaaaa cccctttact
     1561 tcttcatgtc tggaaaccgg atgataaagc ttgtaccctt ccccttcccc agctcgatgg
     1621 atcccccgag ctggctcacg gcaaggccat ggaccagatg gaggcccagg gtcccggtct
     1681 cctccaggtt gaaacccggc ggaagcccaa tcccgtcgtc ccggatctcc atcacgaagc
     1741 ccttcccctc ctccctcgca acccggatgc agatcttccc gcccttttcc ccggggaagg
     1801 catacttcag ggagttgctc acgagctcgt tcatgatgag gctcacgggg agggcggtgt
     1861 cgagatcgag ggagatatcc cggacatcca tctggaggga aacacctttc gaccgctctt
     1921 cgtacgagcg gaaaaggtac tggaggagcc gggtcaggta ctcggcaagg tcgatggtgc
     1981 cgaaggaccg ggactggtag agcttttcgt ggatgagggc catggtcttt atccggtgct
     2041 cgctctcccg gaacatctcg aggatcctcg ggtccttcgt cttcttggac tggaggatga
     2101 ggagggcgga gaccacctgc atgttgttct tcacccggtg gtggatctcc cggagcaggt
     2161 tctccttctc gaggagggtt gcccggatct gggcctccgc cctcttgagg atggtgatgt
     2221 cgatgcagac cgagacggcg gccttcacct ttcctgcctc gtctctcagc gggtacatgt
     2281 tgaggagggc agggaactgc tccccgttct tccggacgag ggtgatctct cccgagtact
     2341 ccacctgctt cctcttcatc ttccggtgga tctccgcgag cagttcgagt tcctcggggg
     2401 ggtagaggag ggcgagcttc tcgccgacaa tatccccctt ccagtacatg aagatgccct
     2461 ctgcgcccgg gctgaaatcc aggatccggc tctccttccc ccggaggtcc gaggtcacga
     2521 aggcgacgtt ctgggcggcc tcgaagatgg ccgagagccg gcgttcacgc tcggtaagtt
     2581 cccggtcccg ggagaggctc atcttcatgg cccggaggaa gatggcgagc agccgatcct
     2641 cctggtcact gcgagcctgc ccggccctcc tgttccctgt cacctcacgg taggtatcga
     2701 gccttccgcc ctggtacggg gcgtctctca ggggcaacgt tatgtacgag aaccaccgga
     2761 ctcccttcgg cccggagagg gcaaactcga ttccctttcc ctctcctccc gtggagagga
     2821 tcccggtcag cctccgaatt cctccctcgt cccccgccag gtaccgggcg atccccttcg
     2881 cgataaagtc ccgggcggtc atgccccggg actcctccgc cctcatcccc gatacctgct
     2941 ccatcgcgga gttgaggtgg acgatctttc cggacccgtc gatgagaaga aggccgtcgg
     3001 gaaggaggtc gagcaatccc ccggtgatgg catcatcccc cggggcagtc tccctctccc
     3061 ggggcgtact cctagtcaaa ccagccccca tgctcatatg tgctgaagag aaccataaac
     3121 tgagtacctc ccgcttgggg gggtcgcaca tgttcctgtc cagacctgcg gcaggggcac
     3181 ttcatccctt ctccctcccc ttccgcctct ccgtcggagg acagtcctgc ggcctcccgc
     3241 ccctccatgc cccggtcgga acggcccggc tctggtttca cccataaagg ttatcatttc
     3301 tcgttaaccg gttgtgcacc tcatcaaccg ataataatat tggcaaatcg acccaatcag
     3361 gaattaatca aaaatataaa ataagtaatc tttatataca tgtgttttga tatagattgt
     3421 cttgcagaga acggtatccg ggggtctctc ttcggctatc gggatggaga tcagtcatga
     3481 tgttcccata tgagatagaa aatctcctca acatggatgg gttgcaggac atggatgaga
     3541 ttctggaccg gctcctgaag gtccgcgata tttcccggga taaggttcgg gaccttggtt
     3601 ttgacatccg gtccgtggac ctggacaacc tcccggttga gctcgacaag gaaaaggtct
     3661 actggatctc gttttacaag aacctcttca acttcatcag catccccctg ttcgtgaacg
     3721 gtgtcatcga ccagcaggag atcgcgagca tcaggttcga tgagcactgc aagcacgaca
     3781 tcgccgagat cctgacgagt atcgatgtgg aggcatcctg atctcctccc ccctcctcct
     3841 tcattcacaa ctccggaaat accatatcat cccttttcgc tgatttcacc agaaggggac
     3901 cctccgcccg ggttcacaac gtttaagtcc gttccccggt aacagttagc ataccgatcc
     3961 ggggaggccg ggttccagat gctcgacgtg gggaaggtca cgaaactcat cggaggcatg
     4021 accatccttt ccgaggtgag cctctcggtg cagaagggcg agatcgtcgg ggtcgtcggg
     4081 ccgaacgggg cagggaagac cacccttttc aatgtcatca ccggcatttc ccggccgaac
     4141 aggggatcca tctcattcga gggcgaggag atcaccggcc tcacccccga gcagatctgc
     4201 aggaagggag ttgcgaagac cttccagatc gctcactgtt tccccaacct cacggcccgc
     4261 gagtcggtca tggtgggggc gatcttcggg acggacgggg taccgtccat gggagaggcc
     4321 ggggaggaat cggagaagat cctcgggtac gtcggcttcc ccggggaaaa ggcagacacc
     4381 ctcgtcagga acctgaacgt cgtcgagtta aagaaggtgc agctggcccg ggcccttgca
     4441 tcgaaaccga ggctcctcct cttcgacgag ttgatgaccg ggctgaacac ggccgagagc
     4501 aaggattcgg tggagctcgt gaagaagatc cgggaccagg gtatctccat ccttatgatc
     4561 gagcatgtca tgagcgtcat catgggggta tccgaccggg tcgtggtgct cgaccatggc
     4621 gaaaagatcg ccgatggtca cccgtacgaa gtgatcaacg accagagggt catcgatacg
     4681 taccttggcg acgtggacgc attctaggag aggggacccg gtgctcgaga tagagaagct
     4741 caatatcttt cacggaaacc tccaggtcgt ctgggacctc tccctctcgg tgaactccgg
     4801 ggagctcgtc accctcatcg gcccgaacgg ggcgggcaag acctcgaccg tcgagaccat
     4861 catcggcctc aacccccggg cgaagggaag catcaggttc atgggagagg agatcctggg
     4921 gatgcccccc caccggatct tcgggaaggg catcgccctc gtcccagagc agagggagat
     4981 cttccccaag atgcgcgtct cggagaacct gcagctggga gcccatggca gggccggctg
     5041 ggagaagaaa ctggaacatg tcttttccct cttccccgtc ctttcggagc gggagcgcca
     5101 ggtggcgggg accctttcag gcggagagca gcagatgctc gccatcggga gggccctcat
     5161 gtcggagccg aggatgctcg tcctcgacga gccctccacc ggtctctcgc cgatcctcgt
     5221 agggcaggtg ttccgctccc tggagcagct cggccgggag gggctgaccg tcctcctcct
     5281 cgagcagaac gtccgccacg ccctcgagct ctgcaccagg gggtacgtgc tcgagaacgg
     5341 gaggatcgtg ctccagggga agacgaggga tctcctccac gacccccaca tcgagcgggc
     5401 atatctcggg atctgaggac cggttgcgcc ccatcacgag ccccgacttt tcaggaatga
     5461 tctgtgcctc cgggaaaggg cgtatcctac ccggggaacg acgctcggcc accccacccg
     5521 gttacggtag accggtcgga tgtgcccggg gttccccctt gcgagggaca cggcagcggg
     5581 agggtgagta gtgaagcaaa accccgagaa tctcccgcca ctccgagaag ggggggaatt
     5641 ccccccggtt tatccgtcac tccctctctc gggtcccggg tttgccttcc tcgattctct
     5701 ccatcttcgg aactcttcgg ggagtgacac gagcccccgg gggaggaaga tcaccacgac
     5761 gatgagcagc acgccgatga cgacgaaccg ctcgaaggtg aggcccagcc ccttcaggcc
     5821 ctcccagatg acggtgagga cgacggtgcc aatgacgggc ccgctgatgg tcccgagacc
     5881 gccgaagatc gagtagatga cgggccagaa ggacgtctcg gcatggaata tctccgggga
     5941 gatgaagctg atgtggtgga cctggagcgc tccggcgacc cccgcgatga agctgctcac
     6001 ggtgaaggcc agcagcttgt accggacggg attcaccccc gatgcccgtg cctccagttc
     6061 gttctccctg atggcggcga aggcaagacc catgcgggac cggaggatga ggtggatgca
     6121 gaggatggag atgacggtga tggcgaggat ggcatagtac tcgtaggcca ggtagccctg
     6181 gatctcggcc gggagcagcc gcggcgagcc gatgccgtcc cacccgccgg tggtggcggc
     6241 aagagggctt gcgatgacgg cctggaggat cacggcaaag ccgaaggtca ccatggcaag
     6301 gaaccactcc ttcagccgca cgcaggtgag gccgaggagg agcccgataa cggccgcgac
     6361 cgcccctccc atgaggatgg tgccgaaggg ggagaacccc gtcgcccggg cgatgagagt
     6421 ggatacatac cccccgatgc caaagaacgc ggcgtggccc agggaggcct gtcccgaata
     6481 ggcgaggaag ttccaggccg aggagaagag gacgaaggta agcatgatga tgaccacgtt
     6541 caggacatag aagctcatcc cggcgatcag ggggaggagc accgcgatgg cgagagcgat
     6601 tcccgtgaga taccaggaag aacgacccct ctccacccgc atcactcgcc cgcctccccg
     6661 aagagaccgg tcggcctgaa gatgagcacc acgatgagga tcagaaacga gatggagtct
     6721 ttccagatcc cgcccaggaa gtacgcagcc ccgttctcgg cgatgccgta gaggaggccc
     6781 cccacgatgg cgccggggat gcttccgagg cccccgagga tgatgatggc aaaggccttg
     6841 atggccggga tggaaccgat gtacgggttg aagacctgcc cgctcaccac ccacagggtg
     6901 cccgcggcgg cggcgagacc ggacccgatg ccgaagctga tcatgtccat tctctccacg
     6961 tcgatcccca cgatcatggc tcccttcctg ttctgggagg tcgccctgat ggccatcccg
     7021 agttcggtcc tcctgagaag gaaatagagc ccgcagagga tcagggccgt gatggcgata
     7081 atgatgatgt agtcgagggg aagccggatg gccccgatgg gaatggagat attttggtac
     7141 gggctgttga ggacctgcct gtcgtctccc cagatgaggg cgaccccgtt ctggaagatg
     7201 tagatgagcc cgagggagag gaagatctcg ttcagtttac cggtgccgag gatgggccgg
     7261 aagcagagcc gctcgaccag gatcccgatg aggcccaggg cgagcatggt gaggagaatg
     7321 atgacatacg ggttgaggcc ggagaggaga aagacggagg ccgtgatgta ggcgccgagc
     7381 atgaggacct cgccgtgggc aaagttcacg atcttcatca cgccgaagat caggttgagg
     7441 ccggtcgcga ggaggatata gatgcacccg gcgtacaggc cccagaagag aacctggatc
     7501 aggataccga gatcgagagc cacgattcct ccgtccctcc ttgtacagta tcgaaggcag
     7561 gacgtgataa aagaggggga tttatccgga cccggcctgg taccagtccg gcaggacgaa
     7621 ttcctgctcc ttcagttcat cgggccagac gatcttgggc ctgacctctc ccgcctgggc
     7681 attccagatg agctgttcca tgaagagcga gaactggacc tcgcggtagt ccttggagaa
     7741 ggtgatggtc cggtccttga tggcctcgac gatctcgggc atcgagaggc tcgcgagggc
     7801 gtctctcacc gcggccttgt ccagggttcc cgcattctgg attgcctggg cctcgatgta
     7861 gacaccctcg taggtcgagg tacccatcat gtcggggaag gtcccgtact tcgcctggta
     7921 gtctgtcttg aacttgacat ccgcggtggc gatggcagat gccggtgtcg cgtacggcga
     7981 gaatcggctc tcgatgatgg aatactcccc gaagtgcccg attcccttgt agtagtcggg
     8041 gttgtcgttc gtctcgaccg cgaggatgat ggtgttcagg ttgacgtccc tccgggcctg
     8101 gacatctatc tgggtctgtt ccgccgggaa ggccgcggca tagatggcat cgggcctcgc
     8161 tgccttaatc gccgtgaggg ccgtcctgaa gtcggtctcg cccagcttga agctctggtt
     8221 cgagacgatc tggatgttca ggttatttgc ggtgatggta ttggtgacgc cggcgagcac
     8281 tcctttcccg taggcactat cctggtagag gatggcgagc ctcatgggcc ggctgctgtt
     8341 gaactcgaac ttctcgttga tggccgggcg catgacctga tccatgaaga gcgtcgtgta
     8401 ctgcccgtac atggcggtcg tcgggcagaa gtggaacatg tagctcgtgt ccacgcccgc
     8461 cttccgggtg acatcggggc tcgaggcacc ggtgaccacg tacgggatct tgttctcggc
     8521 gaccacgccc tgcgtggcga tggtcaccgc gctgctgtag cccccgacga gaagatccac
     8581 cttatcctcc gatgcgagct tggtcacggc cttcaccgcg ctgtcgggct ttgtctcgtc
     8641 atcggccacg atgagggtga tggggagctt cttgttatat gccttcacaa acacccctcc
     8701 ctgggcattg atctcgctga ccgcgagctc ggcggactgt ttcatgtgct ttcccacgtt
     8761 gctccctgca cccgtcaggg agaccaccgc tccgatcttg atgtcctggg gctgggcctg
     8821 ttgctgtgcg cagccggcca ggagcaccga gcctagcacc agcgccgcga gcagaatgga
     8881 taccgttgtt tttttcatgc cgtttcctct gtgcagaagt gccatgtcaa tgcatgggct
     8941 gccatgtcat ggcatgacag tggtataccg tgggaaccgg ggaaatataa cggttgttat
     9001 tcttgacgga gacggggagt gtctggcccc gagaattttt cgcatccgga tgagttccca
     9061 catctcaccg tatgcgcatc ggggaacatt ttcaatattc cccctggcat ccctgcacca
     9121 tggacggctt catgtacggg atgatgtacg ggaatccgta cggctgggcg ttcccctgga
     9181 tgatcgtggc ctgagtcctc cagctggtcg tcgggtactt cgtctaccag gatgcaaggg
     9241 ggaggcagat gagcgcggtg ctctggttca tcctcgtaat cctccccatg atcgggtatc
     9301 tcttcctcgt ggtatacgtg atcatacgga aacccgggag cccgctgcgg gtcccgccgg
     9361 gaaatctgcc ccgcagatcc tggacgagag gtacgcgaag ggagagatct ctcccgggga
     9421 ataccagcgg atgaaggaag acctgaagaa gtaggggggg agctgccccg ggcgaaagga
     9481 cccgggaatc cggaggcaat cctcccggct tttacgcctg aaaacgcgcc ggtttcacga
     9541 actttcgatt attctcaatt caata
//
LOCUS       DATGDI010000111        17166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_330593, whole genome shotgun sequence.
ACCESSION   DATGDI010000111 DATGDI010000000
VERSION     DATGDI010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 17166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17166
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330593"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1956)
                     /locus_tag="VKO45_04220"
     CDS             complement(43..1956)
                     /locus_tag="VKO45_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HMA05114.1"
                     /translation="MGEKQVSRYEEAYRRSIEDPEGFWGEAAGAVSWYRKWDRVLDRS
                     NPPFYRWFPGGIVNTCYNALDRHVEAGHGSRPALIYDSPVTSTVKTFTYAGLLDLVSR
                     CAGSLARLGITRGDRVIIYMPMVPEAVISMLACARIGAIHSVVFGGFAAPELATRIDD
                     ARPRLVISASCGIEVNRVIPYKPLLDRAIEIARAKPERCVILQRPQLESSLKPERDIP
                     WEEFMSGEPAPCVPVGATDPLYILYTSGTTGIPKGVVRDNGGHLVALTWSMKNIYGMD
                     PGEVYWAASDIGWVVGHSYIVYGPLFHGCTSILYEGKPVSTPDPGAFWRVISDHGVAA
                     LFTAPTAFRAIRREDPEGEHIRRYRRTGFRTLFLAGERCDPDTLLWAEKQLGVPVIDH
                     WWQTETGWAIGGNPVGLEMLPVRPGSCTKPIPGYDIHVLDGEGRELPAGKIGNVVVRL
                     PLPPGCFPTLWQNDRDFVRTYLADYPGYYATADAGYLDTDGYLWIMSRTDDIINVAGH
                     RLSTGGMEEVLASHPDVAECAVTGVNDEIKGEIPVGFIVLKAGVSRPEAVIVEELVRL
                     VRERIGPIASFRQAAVVKRLPKTRSGKILRGTMKKIADGEPYAVPPTIDDPDILDEIR
                     ESLAALGYPRGNP"
     gene            complement(2395..3633)
                     /locus_tag="VKO45_04225"
     CDS             complement(2395..3633)
                     /locus_tag="VKO45_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HMA05115.1"
                     /translation="MGENPWQGQDPPNNEEIYKYLLEKISQLENRNLELREQLRQLEV
                     EKRYTETQKIRYERDVRKLKSEIEQLRSPPLVIGTITDVVDANRVIVRSSSGPRFLVR
                     TSQCIEPDQLKPGVRCSLNQQSLAIVEILPSSYDAAIYGMELVESPAETYADIGGLEK
                     QIVEIKEAVELPLKKPNLFKEVGIDPPKGVLLYGPPGTGKTLLAKAVAHETNAHFLRV
                     VGSELVQKYIGEGARLVRELFDMAQKKAPSILFIDEIDAIGAQRMDADTSGDREVQRT
                     LMQLLAGMDGFDRRGDVKIIGATNRIDILDRALLRPGRFDRIIEIPLPDADGRFAILA
                     IHTKGMNLDKSVSLKEVARATDGKNGADLKAICTEAGMFAIRSDRTTVSQQDFMAAIE
                     KVSIDFSKHQLTTSYGAMFA"
     gene            3688..4185
                     /locus_tag="VKO45_04230"
     CDS             3688..4185
                     /locus_tag="VKO45_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein bridging factor aMBF1"
                     /protein_id="HMA05116.1"
                     /translation="MQCELCGGEIRGAPRTVHIEGAELRVCAPCSKYGTEVQQPRAQA
                     GSPVAPRRAGGPPAPQPPVQRRRRDLFDRMEGDIAEDYADRIRNARAAKGWTQKQLAL
                     EIMERELLIKKVEKGDLIPEEELRKKLEKVLGISLLESAKEEVKSPKGKGITTTFGDI
                     ISIKK"
     gene            complement(4466..6571)
                     /locus_tag="VKO45_04235"
     CDS             complement(4466..6571)
                     /locus_tag="VKO45_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMA05117.1"
                     /translation="MTGPGDEPDTWAIFGGARDPQGLVEEGIEFYTRGKYTDALDRFN
                     AALEEDPSSPAAWLNKGGALTVLGRYEEAHQAFERALELDGGNPLVLRNKGITEIYLG
                     RLGDAILSFDRALEIDPYDVTALLNRGIALVKVGRHAEGIECFDLVLDMDLRNTIAWV
                     QKGKAMAIMGKGREAIELFEKALALNPRDPEAWLHKGLALAGMDEKDKALAAYDRALE
                     ISPHLLPALTAKGILLIKKGDYGGALAVMDEAIALDSSDPNAWFGRGVTLKILGRYDD
                     ALASFDRALAINPKDASVYQGKGDLHVLAGRYKEALESYLKARDLDPSLAGPLEGAIE
                     RCITFVQFNLAQWRDQGLFLLSQDEPVEAIRFFDRVLEVEPEHAEVLLRKGEALEALG
                     RNEEALGSLDRLVQVSPRDTAGWLARSDVLIRLGRPEEAAVSLDGVIHDHPEMKDAQA
                     REAYTRSGDAMTVRGNFAGALSAYESALAMAPTNPQALVGKGRALVMLDRPVEAASVF
                     DRALMLEPENPDIWVKKGIVLDIMGRFDEAVACYEHALALRPRDADSWFNLGLTLLNK
                     SEFEEAVKAFDHALEADPRNAEAYYNRGVALLGLRKYEKSLMSFNKAVEIDDTVPEFW
                     HSKGCVHGILRDFNEAMRDIDKAIALNPKFALAWRSRGLFLQFLGKLPEAVTSYERSL
                     DLEFDIETEQMKDTIRGKI"
     gene            6773..7462
                     /gene="tpiA"
                     /locus_tag="VKO45_04240"
     CDS             6773..7462
                     /gene="tpiA"
                     /locus_tag="VKO45_04240"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HMA05118.1"
                     /translation="MGEPFILINCKALPQGMGSRGQIIAEAAETVAEESGVRIGMAPV
                     YTEIHPISHHFRIPVFAQHIDGVPPGPFTGHVTAAAVRHAGAVGTLINHSERRLTLAE
                     IEACVKAAREAKLETVVCTNNDPTTAAASALGPDYVAIEPPELIGSGVSVCTADPGII
                     RRSVEAARRVNPGVKVLTGAGIQTGDCVRIALDLGTTGVLLASSVVKAEDPAKVLRDL
                     VSQVGVTKKAG"
     gene            complement(7443..7997)
                     /locus_tag="VKO45_04245"
     CDS             complement(7443..7997)
                     /locus_tag="VKO45_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA05119.1"
                     /translation="MHIPTPDEIRARRMNLKLTQTELAMKAGISQSMIARIESDSVDP
                     RVSTLRKIVDVLSESGKPPYRAKDVMHTPVYAVGPADPVSLAVEIMERNDVSQLPIID
                     GGVPIGCISESAIVSALEAGGMHASGSRPVREFMEPGFPTVPLEADVRDVVHLLRENH
                     AVLAVEGGRVAGVITKHDLIPLFS"
     gene            complement(8050..8350)
                     /gene="rnpB"
                     /locus_tag="VKO45_04250"
     ncRNA           complement(8050..8350)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VKO45_04250"
                     /product="RNase P RNA component"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00373"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00373"
     gene            8460..9026
                     /locus_tag="VKO45_04255"
     CDS             8460..9026
                     /locus_tag="VKO45_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HMA05120.1"
                     /translation="MKALTEEEGTAALSLARQALEASVRHGKFSLPPLPPVFSEKRGV
                     FVTLTEAGDLRGCIGLPYPVMALREAIPEAAASAALQDPRFPPVEPEDLPRIHVEVTV
                     LSVPEPMGGSPEGRENCVRVGVHGLIAQGMGRSGLLLPQVATEYRWTACEFLDHTCVK
                     AGLPPGSWKSPRVEVLTFEGQIFDEKEP"
     gene            9026..10477
                     /gene="tgtA"
                     /locus_tag="VKO45_04260"
     CDS             9026..10477
                     /gene="tgtA"
                     /locus_tag="VKO45_04260"
                     /EC_number="2.4.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(15) transglycosylase TgtA"
                     /protein_id="HMA05121.1"
                     /translation="MAIRFELLEKDIAGRLGRLQVGEKTVHTPALFPVVNPHLPLIPP
                     AEIQAMGAEAIITNAYIFATSDRFRERALEEGLHRVLGFHGVIMTDSGAFQQSVYGDV
                     EITNRETLLFQRSTGSEILVPLDIATPPRADRRTAESDLIVTLERIREAVDTVGVENL
                     AAPVQGGRFPDLREEAGRAVSGMGFGFCPIGGVVPLMETYRYRELVEVVLAAKRGLSP
                     AACVHLFGAGHPSMLALAVAMGCDVFDSAAYALFAREGRYLTPSGSLHIGEITELACP
                     CEACRGRSAEDLRGDPGKEALLARHNLLVTLGEMARIREAIREGTLWELVDDRCRSHP
                     RLLDGYRALLRHGEELEQSDRASKRRFFYRGSESCLRTEVHRYHRLVGRVPAAGQVLV
                     SLTGKVPPGYDTAFLFRPPFGPYPQELGETFPIGQSEIPSWDREMVARGCAGIRALLK
                     DHEGIHLTVLTQPEWGDLVKGEVPGAEVLIEPL"
     gene            10464..12095
                     /gene="arcS"
                     /locus_tag="VKO45_04265"
     CDS             10464..12095
                     /gene="arcS"
                     /locus_tag="VKO45_04265"
                     /EC_number="2.6.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002948 - archaeosine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0002927 - archaeosine-tRNA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine synthase subunit alpha"
                     /protein_id="HMA05122.1"
                     /translation="MSLFDIRRRDCLAREGTFSREGYSVRTPGIIDIPDVFPGLDGET
                     GTNVPLSADAEFVSRYHHPGTESPVVVHPLGREEVSPGDLVIVANWQTTLSRPRDFVR
                     YLVSLKGRLPPDTAWYAPASALPSNAALLAYVGFDLFDACAVDLATVRGYFCTPEGIL
                     PEDLTGAGVCGCEGCLSGDIGRHNRIALERELALVRRFIAESRLRDLVEARCRSDPDQ
                     VAILRLLDREESFLEPYTPVARSGSLLATTTEVFRRIEVVRFGRRVVERSLPPVHDVA
                     VLLPCSARKPYSTSLSHHRFMKAIRGRAHELIVTSPLGLVPRELERVYPAAHYDIPVT
                     GHWDREELALVSSVIAGYLARHPYRRIIAHLEGGALVAAQMAADACGISLEVTAGPHP
                     ADPASLAALDAALEGERRRSSDMVRGVLAWQLGVAVETRGMTVRGRPPQERVEKAGKI
                     LFTIDAGTGLLRPTFDGWALLPEVYRVRIDAFTPKGDILAPGVISADEGIREGDEVLV
                     LGKGLSATGRAAMGGPEMSRSRHGVAVRVRKLKSE"
     gene            12141..12974
                     /locus_tag="VKO45_04270"
     CDS             12141..12974
                     /locus_tag="VKO45_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HMA05123.1"
                     /translation="MYRVEEVFRFSERVFQMWIRAPQVARNAAAGQFLVIRLDECGER
                     IPLTVSAVKGDRVRVIFMTVGKTTTRLSLLSAGEDIRDVVGPLGKPTEAKTYGTCAVI
                     GGGVGIAGAPIIARALRGAGNRVIGILGARSAPFMILEEEMRESCDEVLTATDDGTLG
                     RKGFAADLLKEVMAREHLDAVWIIGPAIMMKVTSDVTRPQGIRTFVSLNPIMVDGTGM
                     CGSCRVLVGGKTRFACVDGPEFDAHQVDFDALMSRQRMYLDEEKRAVEHRHAGPCGAG
                     R"
     gene            12971..14320
                     /gene="gltA"
                     /locus_tag="VKO45_04275"
     CDS             12971..14320
                     /gene="gltA"
                     /locus_tag="VKO45_04275"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HMA05124.1"
                     /translation="MTGGGCGEGNLEGNFSEVEKGFSADEAVEEAGRCLACKKPGCVG
                     GCPVSIDIPAFVQAVAEGDFRAAARILKAENMLPAICGRVCPQEIQCEGVCILGVKDR
                     PLAIGKLERFVADWERESGVELPPVAPPTGRRVAIVGSGPSGLTAAAELARSGHAVTL
                     YESLHEAGGVLMYGIPSFRLPREVVKTEIESVLALGVDLRLNHLAGKSVPIGDLLAFD
                     AVYVATGAGLPFFMNIPGEHLNGVYSANEFLTRVNLMHADRFPEYDTPVRRGCHVAVV
                     GGGNVALDATRVARRMGARATLIYRRREEDMPARRAEIHHAKEEGVEFLTCANPVRVI
                     GKDRVEGVECHRMRLCGEEDGRGWFEPVEGSEFTVEADVFIVAIGTSPNPLLPRCLTG
                     LECGRLGNVVVDGEGRTSIPRVFAGGDVATGAATVILAMGAAKTAVKAMNRMLGGGD"
     gene            14391..15374
                     /locus_tag="VKO45_04280"
     CDS             14391..15374
                     /locus_tag="VKO45_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HMA05125.1"
                     /translation="MKGRILVHGGVGRWDAEEGREGEVVPVLTGAIEAGLSAMRDGTA
                     VDAVVEAVATLEESGVFIAGRGALPNLDGDFELDAGLMDGETLRVGGVAAVRSVLHPI
                     RLARVVMERTENILVVGHGAGRLAGAFGLSGEIRPDASRREEIERKRREYLESPDHRW
                     VHEPGPGPTRAREGDTVGAVAMDGEGRFAAATSTGGLAFKIPGRVGDSPVVGQGFYAM
                     RGAGAASASGIGEAIARYGLSLRAVLRMAGGMRAQAAAEVEISGLSRLFGPDTAGIIL
                     LDRDGTPGVSFNTRGMAVGFGKDGSGSHSHIVRREELPVFTPALRKEMGSG"
     gene            complement(15430..16509)
                     /locus_tag="VKO45_04285"
     CDS             complement(15430..16509)
                     /locus_tag="VKO45_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HMA05126.1"
                     /translation="MVKLTIRGGDLDLVEYDLQPFRPGEQMNTLGFEKKKFSLKPVKG
                     TVRVKAPARIHLTVLDMNRFAPGKPGGGGVGFALQVYCTAEVSCIPKGVEIDYNRAPV
                     LRHHVEIFRKAVGYRGGFRIKARDHEYHHVGMGSTSTISIAALNAMNRAVGSPLNSDE
                     LRLLMGYNFVEETGEGKIAFAFETGVGPAVSSHGGMAVMGDDLALVYRHAFAEGQKVF
                     IVIPTSDISSAGTREIELLMNRGRSLDYMDREVKCHMVMMDLIPALERGDVKKAGKIF
                     WEIEFRGSKRAEVEHHGFRIYQYMSKIREAGFDFVGMSSVGPTIAVITDRSRKDLEKA
                     IKPLNLKVAVETKIDNKGMVIGKKG"
     gene            complement(16518..17069)
                     /locus_tag="VKO45_04290"
     CDS             complement(16518..17069)
                     /locus_tag="VKO45_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HMA05127.1"
                     /translation="MTGTETAAVETVAGLMALSARTAPKAKGQDALVTRVLAGDELKE
                     LSRGMDALGTDRGIRFFLRDARNVGESDACLLLGMRREPTAGLDCGACGYATCAEMLR
                     EDPKKNPTLFSGPNCAVRMADLGIALGSAVKTASIHNVDNRVMFSAGATGLALGLLPG
                     CTVGYGIPLSASGKNIFFDRKDK"
BASE COUNT         2838 a         5523 c         5796 g         3009 t
ORIGIN      
        1 tgatgtgggc ctcggggggc tttcgaaaga ctccttccgg gatcaggggt tcccccgggg
       61 gtaccccagc gcggcaaggc tctccctgat ctcgtcgagg atgtcgggat cgtcgatagt
      121 cggaggaacg gcgtacggct ccccgtcggc gattttcttc atggtccccc ggaggatctt
      181 ccctgaccgg gtcttgggga gcctcttcac caccgcggcc tgacggaacg aagcgatggg
      241 gccgatccgt tccctgacga gccggacaag ctcctccacg attacggcct cgggccggga
      301 caccccggcc ttgaggacaa tgaacccgac cgggatctcc cccttgatct cgtcgttcac
      361 cccggtgacc gcgcactcgg ccacgtccgg gtgcgaggcg aggacctcct ccattccgcc
      421 ggtcgagagc cggtggccgg cgacgttgat gatgtcatcg gtccggctca tgatccagag
      481 gtacccgtcc gtgtccaggt acccggcatc ggccgtggca tagtatcccg gatagtccgc
      541 caggtaggtc ctcacgaagt cccggtcgtt ctgccagagg gtggggaagc acccgggcgg
      601 gagcgggaga cggaccacga cgttccctat ctttcccgcg ggaagttccc gtccttctcc
      661 gtccaggaca tggatgtcgt atcccgggat cggcttcgtg caggacccgg gcctgacagg
      721 aagcatctcc agcccgacgg gatttcctcc gatggcccag ccggtttcgg tctgccacca
      781 gtggtcgatg accgggaccc cgagctgctt ctcggcccag aggagggtgt cggggtcgca
      841 ccgctcgcct gcgaggaaga gggtccggaa tccggttctt cggtatctcc ggatgtgctc
      901 cccctcggga tcctcacgcc ggatggcccg gaaggcggtg ggggcggtga agagggccgc
      961 aaccccatgg tcggagatga cccgccagaa ggcgccgggg tcgggggtcg agaccggctt
     1021 tccctcgtag aggatggagg tgcacccgtg gaagaggggg ccgtacacga tgtaggagtg
     1081 gccgacgacc cacccgatgt ccgaggcggc ccagtagacc tcgccggggt ccatgccgta
     1141 gatattcttc atggaccagg tgagggccac caggtggccc ccgttgtccc gcaccacgcc
     1201 cttggggata cccgtggtcc ccgaggtata gaggatgtag agcgggtcgg tcgccccgac
     1261 gggaacgcag ggggcgggct caccggacat gaactcctcc cagggaatgt ccctttcggg
     1321 cttgagggac gactccagct gcggtctctg gagaataaca cacctctccg gcttcgcacg
     1381 ggcgatctcg atggcccggt cgaggagggg cttgtagggg atgacccggt tcacctcgat
     1441 cccgcaggag gcggagatga cgagcctcgg ccgtgcgtca tcgatccggg tcgcaagctc
     1501 cggggcggcg aatccgccga agaccaccga gtggatcgcc ccgatccggg cacaggcgag
     1561 catcgagatg accgcctcgg gaaccatggg catgtagatg atcacccggt caccccgggt
     1621 gatcccgaga cgggcgaggg atccggcgca ccgggacacg agatcgagga gccccgcata
     1681 ggtgaaggtc ttcacggtcg aggtcaccgg gctgtcgtag atcaaagcgg ggcggctgcc
     1741 atggcctgcc tccacgtgcc ggtccagggc gttatagcag gtattcacga taccgccggg
     1801 gaaccaccgg tagaagggag ggttcgatct gtcgaggacc ctgtcccatt tccggtacca
     1861 gctgaccgcc ccggctgcct cgccccagaa cccctccggg tcctcgatgg acctccggta
     1921 ggcctcttcg tatctcgaca cctgcttctc tcccatggca gatcgcacta aaaaattcag
     1981 ggagggtttt cttatctgtt gtgatttttc caccgggagg ggagattcaa cgattcataa
     2041 ttctgacaga ggatcgggaa cgtcagacgc tcctggggga ctcgcctatc ggctcgcccc
     2101 cgccgctgcg gcgtcccccg cgtggagata acccccgttc tcggactctt tgattcttca
     2161 gttatcggca accatccctt caggaattcc cgtccacgga gggggatggc cgcgccgggg
     2221 ggcggtagcc ctcgcgactt cgtcgctcga ggtcggtttc ttacgaaacc tccctcttac
     2281 gaaacctccc tcccccaaga gcctgacgaa gaagatcaga acaaacaggg aaatgttggg
     2341 tcgatgatca aagaaaatga ataatcggat acctgacaca gaaaaaattg agggttatgc
     2401 aaacatcgcc ccgtacgagg tggtgagctg gtgcttggag aagtcgatgc tcaccttctc
     2461 gatggcggcc atgaagtcct gctgggatac cgtggtccgg tctgaccgga tggcgaacat
     2521 ccccgcctcc gtgcagatgg ccttcaggtc cgccccgttc ttcccatcgg tcgcccgggc
     2581 gacctccttc aggctcaccg acttgtccag gttcatgccc ttcgtatgga tggcgaggat
     2641 ggcgaagcgc ccgtcggcgt cggggagcgg gatctcgatg atccggtcga accgtcccgg
     2701 ccgcaggagg gcccggtcca ggatgtcgat ccggttcgtg gcgccgatga tcttcacgtc
     2761 gcccctccgg tcgaagccgt ccatccccgc cagaagctgc atgagggtcc gctggacctc
     2821 gcggtccccg gaggtgtcgg cgtccatccg ctgggcaccg atggcatcga tctcgtcgat
     2881 gaagaggatg gagggggcct tcttctgggc catgtcgaag agttcccgga cgagccgggc
     2941 accctcgccg atgtacttct ggacgagctc ggaacccacg acccggagga agtgggcgtt
     3001 cgtctcgtgg gccacggcct tcgcgaggag ggttttcccc gtccccggcg ggccgtagag
     3061 gaggaccccc ttcggcgggt cgatcccgac ctccttgaag aggttgggct tctttaaggg
     3121 gagctccacc gcctccttga tctcgacgat ctgcttctcc aggccgccga tgtccgcgta
     3181 ggtctcggcc ggggactcca cgagctccat cccgtagatg gcggcatcgt agctcgaggg
     3241 gaggatctcg acgatggcca gggactgctg gttcagggaa cacctgaccc ccggcttcag
     3301 ctggtcgggc tcgatgcact gggaggtccg gacaaggaac ctcggacccg aggaggaccg
     3361 gacgatgacc cggtttgcat caaccacgtc ggtgatggtg ccgataacga ggggcgggct
     3421 ccggagctgc tctatctcgc tcttgagctt ccggacgtca cgctcgtagc ggatcttctg
     3481 ggtctcggtg taccgtttct ccacctcgag ctgccggagc tgctccctga gctccaggtt
     3541 ccggttctcg agctgggaga tcttctccag caggtacttg tagatctcct cgttattcgg
     3601 gggatcctgg ccctgccagg ggttctcgcc catcggtggt cctcaaataa ctatatagga
     3661 tgtgactata aagatgtttt cgagaccatg cagtgcgaat tgtgcggcgg agagatacgg
     3721 ggcgcgcccc ggaccgtcca catcgagggg gcagagctcc gggtctgcgc cccctgctcg
     3781 aaatatggaa ccgaggtcca gcagccccgt gcccaggccg gctccccggt ggccccccgg
     3841 agggcgggag gcccgccggc accccagccc cctgtacagc ggaggaggag ggacctcttc
     3901 gaccggatgg agggggacat cgccgaggac tatgcggacc ggatccgcaa cgcccgggcg
     3961 gcgaagggct ggacccagaa gcagctcgcc ctcgagatca tggaacggga actcctcatc
     4021 aagaaggtcg agaaggggga tctcatcccc gaggaggagc tcaggaagaa gctcgagaag
     4081 gtccttggca tttccctcct tgaatcggca aaggaggagg tgaaatcccc gaaggggaag
     4141 gggatcacca ccaccttcgg ggatatcata tcgatcaaga agtaattccg attatccctt
     4201 ttccaaggaa atctaatcgt aattttaaca ttcggacgct cctgggggag ggagggttca
     4261 tcagaacccg acctggagca acccgccatc gggcggggtg cgacccggag ggttcatcag
     4321 aacccgacct ggagcgatga agtcgcgagg ttcgctccgc tcacccccgc cgccgcggcg
     4381 tccccagctt ggggataggc ctgttagaag ggaagtgtga tgagctgagg aggagatgga
     4441 atttgctcaa ataacgggaa tccggtcaga tcttccccct gatggtgtcc ttcatctgct
     4501 cggtctcgat atcgaactcg aggtcgaggg accgctcgta ggaggtgacc gcctccggga
     4561 gcttcccgag gaactggagg aacagcccgc gggacctcca ggcgagggcg aacttggggt
     4621 tgagggcgat ggccttgtcg atgtcccgca tggcctcgtt gaagtcccgg aggatcccgt
     4681 ggacacagcc cttgctgtgc cagaattcgg gcacggtatc atcgatctcc accgccttgt
     4741 taaaggacat gagggacttc tcgtacttcc tgaggccgag gagggccacc ccccggttgt
     4801 agtacgcctc ggcgttccgg gggtcggcct ccagggcatg gtcgaaggcc ttcaccgcct
     4861 cctcgaactc gctcttgttg aggagggtga ggcccaggtt gaaccaggag tccgcgtcac
     4921 ggggcctgag ggcgagggca tgctcgtagc aggcgacggc ctcgtcgaac cggcccatga
     4981 tgtcgagcac gatccccttt ttcacccaga tgtcgggatt ttccggctcg agcatgaggg
     5041 cccggtcgaa gaccgaggcc gcctcgacgg ggcggtcgag catgacaagg gcccggccct
     5101 ttcccaccag ggcctgcggg ttcgtcgggg ccatggcgag ggcgctctcg taggccgaga
     5161 gggccccggc aaagttcccc ctgacggtca tggcatcccc cgaccgggtg tatgcctccc
     5221 gggcctgcgc atccttcatc tcgggatgat cgtggatcac cccgtcgagg gagacggcgg
     5281 cctcctcggg cctcccgagc cggatgagaa catcgctccg cgcgagccac ccggcggtgt
     5341 ccctcgggct gacctggacg agccggtcca ggctcccgag ggcctcctcg ttccttccca
     5401 gggcctcgag ggcctccccc ttccggagga ggacctcggc atgctccggc tccacctcga
     5461 ggacccggtc gaagaagcgg atggcctcca cgggctcgtc ctgggagagg aggaagagcc
     5521 cctggtcacg ccactgggcg aggttgaact gcacgaaggt gatgcaccgc tcgatggccc
     5581 cctccagggg accggcgagg gacgggtcga ggtcccgggc cttcaggtag gactccaggg
     5641 cctccttgta ccgcccggcg aggacatgga gatccccctt cccctggtaa accgaggcgt
     5701 ccttcgggtt gatggccagg gcccggtcga aactcgcgag ggcgtcatcg taccgcccga
     5761 ggatcttcag ggtaaccccc ctcccgaacc aggcgttcgg gtcgcttgaa tcgagggcga
     5821 tggcctcgtc catgaccgcg agggcccccc cgtagtcccc cttcttgatg aggaggattc
     5881 ccttggccgt gagggcaggg aggaggtggg gggagatctc cagggcccgg tcataggcgg
     5941 cgagggcctt gtccttctcg tccatgcccg cgagggcgag ccccttgtgg agccaggcct
     6001 ccgggtccct cgggttgagg gcgagagcct tctcgaagag ctcgatggcc tcccgcccct
     6061 ttcccataat ggccatggcc ttccccttct gcacccaggc gatggtgttc cggaggtcca
     6121 tatcgaggac caggtcgaag cactcgattc cttccgcgtg cctgcccacc ttcacgaggg
     6181 cgattccccg gttcaggagg gcggtgacgt cgtagggatc gatctccagg gcccggtcga
     6241 aggagaggat ggcatccccc agcctcccga ggtagatctc ggtgatgccc ttgttgcgca
     6301 ggacgagggg attcccgcca tccagctcca gggcccgttc aaaggcctgg tgagcctcct
     6361 cgtacctccc gaggacggtg agggcaccgc ccttgttgag ccaggccgcc ggggaggacg
     6421 ggtcctcctc gagggcggca ttgaaccggt ccagggcatc cgtgtacttt cccctcgtat
     6481 agaactcgat gccctcctcc acgagcccct ggggatcccg tgccccgccg aagatcgccc
     6541 acgtatcagg ttcgtccccc ggcccggtca tggaagccgg tcccccttcc ctttcctctc
     6601 atcaggcggc accgggcggt gcacctcctc ctttttggaa tacgccgttt cgcggtgaga
     6661 acggaaaaaa cccgtcattc ctgttcccct cgacggagaa aacccctcat acttaggtga
     6721 tcgcactatt ttatatagtt cgtgatgatt cgtgatggta cagggaaccg cgatgggaga
     6781 accattcatc ctcatcaact gcaaggccct tccccagggc atgggaagcc gggggcagat
     6841 catcgccgag gcggcggaga cggtcgcgga ggagagcggc gtgcggatcg ggatggcccc
     6901 ggtgtatacc gagatccacc ccatctcgca tcacttccgg ataccggtct tcgcccagca
     6961 catcgacggg gttcccccgg gaccgtttac cggccacgtg acggccgcgg cggtcaggca
     7021 cgcgggggcc gtggggacgc tcatcaacca ttccgaacgg cggctcaccc tcgcggagat
     7081 cgaggcctgt gtgaaggcgg cccgggaggc gaagctcgag acggtggtct gcacgaacaa
     7141 cgatcccacc accgcggcgg cctccgccct ggggccggac tatgtcgcca tcgagccgcc
     7201 cgagctcatc gggagcgggg tctcggtctg cacggccgac cccgggatta tcaggcgatc
     7261 cgtggaggcg gcgaggaggg tgaacccggg ggtgaaggtc ctcaccggcg cggggatcca
     7321 gacgggggac tgcgtccgga tcgccctcga cctcgggacg accggggtac tcctcgcctc
     7381 gagcgtggtc aaggccgagg accccgcaaa ggttctccgg gacctggtct cccaggtcgg
     7441 ggtcacgaaa aaagcgggat aaggtcgtgc ttcgtgatga cgccggccac ccggcccccc
     7501 tccacggcga ggaccgcgtg gttctcccgg aggaggtgga ccacgtcccg gacgtctgcc
     7561 tcgaggggca cggtggggaa cccgggttcc atgaactccc gcaccggccg gctgcccgag
     7621 gcgtgcattc cccccgcttc cagggcagag acaatggccg attccgagat gcatccgatg
     7681 gggacccccc cgtcgatgat ggggagctgg gagacatcgt ttctctccat gatctcgacg
     7741 gccaaggaga cgggatcggc gggcccgacg gcatagaccg gcgtgtgcat gacgtccttc
     7801 gcccggtagg ggggcttacc gctttctgag aggacatcca ctatctttcg cagggtcgat
     7861 acccgcggat caacagagtc tgactcgatc ctcgcgatca tcgattggga tattcctgcc
     7921 ttcatggcaa gctcggtctg ggtcagtttg agattcatcc gccgggcccg gatctcatcg
     7981 ggcgtcggga tatgcataac agtaatataa aaggggcagt aaataactct tgcgaattct
     8041 tcgaagaatg agtgggagga gtaagccccc ttctgttcat ggcggcattc agctgtgcgc
     8101 caaacctggg cgggcaccag gcggcccatc gcccgtttct ggcttgcacc cgcaggggtt
     8161 caccgtttca acgcttcccg ccgttacgct ccacgaatcg gtcgtgtcat ggctggcggc
     8221 gggccgtgcc gtttctgtgt catggccgtg gctctcgcca cccgcacgtg ggtgcggctg
     8281 cgagcctggt cggtgggggg actttcctca gcggttccga agaaccaccg tcagccgccc
     8341 tctccctctc ccatgaatat gggagccccc cttatttatt tctcctgaag gtccccgcgg
     8401 cagggaaggg gggcaggctc cattcactat tatatcggtg gcccctccat gtatttctca
     8461 tgaaggcgct cacagaggag gagggtaccg ccgccctctc ccttgcacgg caggccctgg
     8521 aggcctcggt gaggcacggg aagttctccc tcccgcccct cccgccggtc ttttccgaga
     8581 agaggggggt ctttgtcact ctgaccgagg caggggatct ccggggatgc atcggtctcc
     8641 cgtacccggt gatggccctc agggaggcga tcccggaggc ggcggcctcc gcggccctcc
     8701 aggacccccg gttcccgccc gtggagccgg aggatctccc ccggatccac gtggaggtca
     8761 cggtcctctc ggtgcccgaa ccgatgggcg gatcaccgga aggccgggag aactgcgtcc
     8821 gggtcggggt ccacggcctc atcgcccagg ggatggggag gagcggcctc ctcctcccgc
     8881 aggtggctac cgagtaccgc tggaccgcct gcgaattcct ggaccacacc tgcgtgaagg
     8941 ccgggctgcc ccccgggtcc tggaagtccc cgagggtcga ggtgctcacc ttcgaggggc
     9001 agatcttcga cgagaaggaa ccctgatggc catccgcttc gagctcctcg agaaggacat
     9061 cgcgggcagg ctggggaggc tccaggtggg ggagaagacc gtccataccc cggccctctt
     9121 tcccgtggtg aacccccacc tgcccctcat cccgcccgcg gaaatccagg cgatgggagc
     9181 cgaggccatc atcacgaatg cttacatctt cgcgacgagc gatcgcttcc gggagcgggc
     9241 ccttgaggaa gggctgcacc gggtcctcgg ctttcacggt gtcatcatga ccgactcggg
     9301 ggctttccag cagtcggtgt acggggacgt ggagatcacg aaccgggaga ccctcctctt
     9361 ccagcgatcc acagggagcg agatcctggt ccccctggac atcgcgacac ccccccgggc
     9421 cgaccggagg acggcggaat cagatctcat tgtcaccctg gaacgcatcc gggaggcggt
     9481 tgataccgtc ggcgtggaga acctcgccgc cccggtccag ggcggaaggt tccctgacct
     9541 ccgtgaggag gccgggaggg cagtctcggg gatggggttc ggattctgcc ccatcggcgg
     9601 ggtcgtgccc ctcatggaga cctaccggta ccgggagctt gtcgaggtcg tcctcgcggc
     9661 aaagcgcggc ctctcccccg cggcctgcgt ccacctcttc ggggcaggcc acccgtccat
     9721 gctcgccctc gcggtcgcca tgggctgcga tgtcttcgac tcggcggcct atgccctctt
     9781 tgcgagggaa gggaggtacc tgaccccgtc gggatccctc cacatcgggg agatcaccga
     9841 gctcgcctgc ccctgcgagg cctgccgggg gcggtcggcg gaggatctca gaggggatcc
     9901 cgggaaggag gccctcctcg cgaggcacaa cctcttggtg accctgggcg agatggcccg
     9961 gatccgtgaa gccatcaggg aggggaccct ctgggaactc gtcgacgacc ggtgccggag
    10021 ccacccccgg ctcctcgacg ggtaccgggc tctgctccgg cacggggagg agctcgagca
    10081 gtccgaccgg gcctccaaga ggcgtttttt ctaccggggg agcgagagct gcctgcgcac
    10141 cgaagtgcac cggtaccacc ggctcgtggg aagggtcccg gccgccgggc aagtcctcgt
    10201 cagcctcacg ggaaaggtcc ctcccgggta cgacaccgcc ttcctcttcc ggcccccctt
    10261 cggcccctac ccgcaagaac tcggagagac cttccccatc ggccagtcag agatcccctc
    10321 ctgggaccgc gagatggttg cccgcgggtg tgccgggatc cgcgccctgc tgaaggatca
    10381 cgaaggaatc cacctcaccg tcctcaccca gcccgagtgg ggggatctcg tgaaaggtga
    10441 ggtgcccggc gcagaggtgc tcattgagcc tctttgatat ccgccggagg gattgcctcg
    10501 cccgggaggg gaccttctcc cgcgaggggt actcggtccg cacaccgggg atcatcgaca
    10561 tccccgacgt ctttcccggg ctcgacggcg agacggggac gaacgtccct ctttccgcag
    10621 atgcggagtt tgtatcaagg taccaccacc ccggcaccga aagccccgtc gttgtccatc
    10681 cgctgggaag ggaggaagtc agcccggggg accttgtcat cgttgcgaac tggcagacga
    10741 ccctctcccg gccccgtgac tttgtgcggt acctcgtctc tctcaaggga aggctccccc
    10801 ccgacactgc ctggtacgcc cctgcctcgg ccctcccgtc caatgccgct ctcctcgcgt
    10861 acgtggggtt cgacctcttc gatgcctgtg ccgtggatct cgccaccgtg aggggatact
    10921 tctgcacccc cgaagggatc cttccggagg atctgacagg ggccggggtc tgcggttgcg
    10981 agggatgctt atcaggggat atcggccggc acaaccggat cgccctggaa cgtgagctcg
    11041 ccctggtgcg gagattcatc gccgaatcga ggctccgcga cctggtcgag gcacggtgca
    11101 ggtccgatcc agaccaggtg gccatcctgc ggctcctcga ccgggaggaa tccttcctcg
    11161 agccgtacac cccggtggca aggtcagggt ccctcctcgc cacaaccacc gaagttttcc
    11221 ggcggatcga ggtggtgcgg ttcgggagaa gggtcgtgga acgatcgctc cctcccgtcc
    11281 atgacgtcgc tgtcctcctc ccctgctctg cccggaagcc gtactccacc tccctctccc
    11341 accaccggtt catgaaggcg atcagaggga gggcgcacga gctcatcgtg acatcgccgc
    11401 tgggcctcgt gccccgggag ctggaacggg tgtaccccgc cgcccactac gatatcccgg
    11461 tcacgggcca ctgggacagg gaggaactcg ccctggtctc gtcggtcatc gcggggtacc
    11521 tcgcccggca cccgtaccgg cgaatcatcg cccacctgga ggggggtgcc ctcgtggcgg
    11581 cgcagatggc ggcggatgcc tgcgggatct ccctcgaggt gaccgccggg cctcacccgg
    11641 cggatcccgc ctccctcgcc gccctcgatg cggccctcga gggggagagg aggcgatcct
    11701 ccgacatggt ccgcggagtt ctcgcctggc agctcggggt cgcggtcgag acccggggga
    11761 tgaccgtgag gggaaggccc ccccaggaga gggtggagaa ggcgggaaag atcctcttta
    11821 ccatcgacgc aggaaccggc ctcctccgcc cgacctttga cgggtgggcc ctcctccccg
    11881 aggtgtacag ggtgaggatc gacgccttca ccccgaaggg ggacatcctc gcgcccggag
    11941 tcatctcggc cgacgagggg atccgggagg gggacgaggt cctcgtcctg gggaaggggc
    12001 tctccgccac cggccgggcg gccatggggg ggccggagat gagccgttcc cggcacgggg
    12061 tggcggtgcg ggtgcgtaag ctaaagagcg agtagatcca acgggtaaaa gaaatttcca
    12121 gtactcccgg ggggaagggt atgtaccgcg ttgaggaggt tttccggttc tcggaacggg
    12181 tcttccagat gtggatccgg gccccgcagg tggcgaggaa cgccgcggcg gggcagttcc
    12241 tcgtcatcag gctggacgag tgtggggagc ggatccccct caccgtctcg gcggtgaagg
    12301 gcgaccgggt ccgggtcatc ttcatgaccg tgggaaagac gaccacccgg ctctccctcc
    12361 tctcggcggg ggaggatatc cgtgacgtgg tgggtcccct cgggaagcct accgaggcaa
    12421 agacctacgg gacctgtgcc gtgatcggtg gaggggtggg aatcgccggg gcccccatca
    12481 tcgcgagagc cctccggggg gccggcaacc gggtcatcgg tatcctcggc gcccgttcgg
    12541 cacccttcat gatcctcgag gaggagatgc gggaatcctg cgacgaggtc ctcaccgcca
    12601 ccgatgacgg aaccctcggg cgcaagggct ttgcggcgga cctgctcaag gaggtcatgg
    12661 cccgggagca cctggatgcc gtctggatca tcgggccggc catcatgatg aaggtcacct
    12721 ccgacgtgac gaggccgcag gggatccgca ccttcgtgtc cttaaacccc atcatggtcg
    12781 acgggacggg gatgtgcggt tcctgccggg tcctcgtagg ggggaagacc cggttcgcct
    12841 gcgtggacgg ccccgagttc gatgctcacc aggtggactt cgacgccctc atgtcccggc
    12901 agcggatgta cctcgacgag gagaagaggg ccgtggagca tcgccacgcc ggcccgtgcg
    12961 gggcggggag atgaccggcg gggggtgcgg tgaggggaac ctcgagggaa acttctccga
    13021 ggtggagaag ggattctccg ccgatgaggc ggtcgaggag gcgggccggt gcctggcctg
    13081 caagaagccg ggctgcgtcg ggggctgccc ggtctccatc gacataccgg cctttgtcca
    13141 ggccgtcgcg gagggggact tccgggcggc cgcccggatc ctgaaggccg agaacatgct
    13201 cccggcgatt tgcgggaggg tgtgccccca ggagatccag tgcgaggggg tctgcatcct
    13261 cggggtgaag gaccggcccc tcgccatcgg gaagctcgag cggttcgtgg ccgactggga
    13321 acgggagagc ggcgtggagc tcccccccgt cgcccccccg accgggaggc gggtcgccat
    13381 cgtcgggtcg ggcccctcgg gcctgaccgc cgccgcggag ctcgcccgct cgggccacgc
    13441 ggtcaccctg tacgaatccc tccacgaggc gggcggggtc ctcatgtacg gcattccctc
    13501 cttccggctc ccccgggagg tcgtgaagac cgagatcgaa tcggtcctcg ccctcggcgt
    13561 ggacctgcgc ctgaaccacc tggcgggaaa gagcgtcccc atcggggacc tcctcgcctt
    13621 cgacgcggtc tatgtcgcga cgggcgccgg ccttcccttc ttcatgaata tcccggggga
    13681 gcacctgaac ggggtctact cggcaaacga gttcctcacc cgggtgaacc tgatgcacgc
    13741 cgatcggttc cccgagtacg acaccccggt gagacggggg tgtcatgtcg ccgtggtggg
    13801 cggggggaac gtcgccctgg acgccacccg ggtggcccgg aggatggggg cccgtgccac
    13861 cctcatctac aggcggcgcg aggaggacat gccggcccgg agggccgaga tccaccatgc
    13921 gaaggaggag ggggtggagt tcctcacctg cgcaaacccc gtccgggtca tcgggaagga
    13981 ccgggtggag ggggttgagt gccaccggat gcggctctgc ggcgaggagg acggccgggg
    14041 gtggttcgaa ccggtggaag ggagcgaatt caccgtggag gccgacgtgt tcatcgtggc
    14101 catcgggacc tccccgaacc ccctcctccc ccggtgcctc acggggctcg agtgcgggag
    14161 gctcgggaac gtggtcgtgg acggggaggg gaggacctcc atcccccgtg tcttcgcggg
    14221 cggggacgtg gcgacgggtg cggcgacggt catcctcgcc atgggcgccg cgaagaccgc
    14281 ggtaaaggcc atgaaccgga tgctcggcgg cggggactga gagcaccggc tggccttccc
    14341 gcacgcatga ccgattctta tgtatcccgt gcaccgcaga agggcatggt atgaaggggc
    14401 ggatcctcgt ccacggcggt gtcggccggt gggacgcgga ggaaggcagg gagggggagg
    14461 tggtaccggt cctcacggga gcgattgagg ccggcctctc ggccatgcgg gacggaaccg
    14521 cggtggatgc cgtggtcgag gccgtcgcga ccctcgagga atcaggggtc ttcatcgccg
    14581 gaaggggggc gctcccgaac ctggacggag atttcgagct cgatgccggc ctcatggacg
    14641 gcgaaaccct gagggtggga ggggtggctg cggtccgctc ggtcctgcac ccgatccggc
    14701 tcgcgagggt ggtcatggag aggacggaga atatcctcgt ggtggggcac ggggcgggaa
    14761 ggcttgccgg agccttcggc ctctcggggg agatccgtcc cgatgcatcg cgccgggagg
    14821 aaatcgagag gaagagaagg gagtatctcg agtcccccga tcaccggtgg gtccatgagc
    14881 ccggacccgg cccgacccgg gcacgggagg gagacaccgt cggggctgtg gccatggacg
    14941 gggaggggag gtttgccgcg gcgacgagca ccggcggcct cgctttcaag atcccgggga
    15001 gggtcgggga ttctcctgtc gtcgggcagg gcttctacgc gatgcgggga gccggagccg
    15061 cttcagcatc agggatcggc gaggccatcg cccggtatgg gctctcgctc cgggctgtac
    15121 tccggatggc cggtgggatg agggcgcagg ccgctgcgga agtggaaatc tctgggctct
    15181 cccggctctt cgggccggat acggccggga tcatcctcct cgaccgcgat ggcacccccg
    15241 gagtgtcctt caacaccagg gggatggcgg tggggttcgg caaggacgga tccggatcgc
    15301 actcgcacat cgtgaggagg gaggagctgc cggtttttac ccctgccctc cggaaagaga
    15361 tgggctccgg ttaaaggccc gggtgcaagg tccccggggc gatgttctct ctcagggttt
    15421 gcccggtttt caacccttct tcccgatgac catcccctta ttgtctatct tcgtctccac
    15481 ggcgaccttg aggttgaggg gcttgatcgc cttctcgaga tccttccggc tccggtctgt
    15541 gatgaccgcg atggtcggcc ccaccgagga catgcccacg aaatcaaagc cggcctcgcg
    15601 gatcttcgac atgtactggt agatgcggaa gccgtggtgc tccacctcgg cccgcttgga
    15661 tccccggaac tcgatctccc agaagatctt ccctgccttc ttcacgtccc ctcgttcgag
    15721 ggccgggatg aggtccatca tgaccatgtg gcacttcacc tcccggtcca tgtagtccag
    15781 gctccggccc cggttcatga ggagctcgat ctccctcgtg cccgcagagg agatgtccga
    15841 ggtcgggatg acgatgaaga ccttctgtcc ctcggcgaag gcgtgccggt acacgagggc
    15901 caggtcgtcc cccatgaccg ccatcccgcc gtgggaggag accgccgggc cgaccccggt
    15961 ctcgaaggcg aaggcaatct tcccttcccc ggtctcctcc acgaagttgt accccatgag
    16021 gagccggagc tcgtcagagt tgagggggga tcccaccgcc cggttcatgg cgttcagggc
    16081 cgcaatggag atagtagagg tggaacccat cccgacgtgg tggtactcgt ggtcccgggc
    16141 cttgatccgg aaccctcccc tgtaccccac cgccttcctg aagatctcga catgatggcg
    16201 gagaaccggt gctcggttat agtcaatctc cacccccttc gggatgcagg agacctcggc
    16261 ggtgcagtac acctgcagcg cgaatcccac gccccctccg ccaggcttcc ccggggcgaa
    16321 ccggttcatg tcgagcaccg taagatggat ccgggcgggg gctttcaccc ggacagtgcc
    16381 cttcacgggc ttcaggctga atttcttctt ctcgaagccg agggtgttca tctgctcacc
    16441 gggccggaag ggctggaggt cgtactccac gaggtccaga tccccgcccc tgatcgtcag
    16501 cttgaccatg atatctccta cttgtccttc cggtcgaaga agatgttctt ccccgatgcc
    16561 gagagcggga tgccgtagcc gacggtgcac ccgggcagga gcccgagggc gaggccggtg
    16621 gcacccgccg agaacatcac ccggttgtca acgttgtgga tcgacgcggt cttcaccgcc
    16681 gagccgaggg cgatgccgag gtcggccatc cgcacggcgc agttcggccc ggaaaacagg
    16741 gtgggattct tcttcgggtc ctctctcagc atctcggcgc aggtggcata gccgcaggcc
    16801 ccgcagtcga gcccggcagt cggctccctc cgcatcccga ggagaaggca ggcatcgctt
    16861 tccccgacgt tccgtgcgtc ccggaggaag aaccggatcc cccggtcggt cccgagggca
    16921 tccatccccc gggagagctc cttcagctcg tcccctgcga ggacccgggt gacgagggcg
    16981 tcctggccct tcgccttcgg ggcggtcctt gcagagagag ccatgaggcc cgccaccgtc
    17041 tccacggcgg ccgtttcggt acccgtcatg tttggagggg tgggcgggag ggaagataag
    17101 ggtttataaa cggggttttc ttacaaccct gaaaaaatct tatcccgaat ctcgcttgtt
    17161 atatta
//
LOCUS       DATGDI010000112         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_98132, whole genome shotgun sequence.
ACCESSION   DATGDI010000112 DATGDI010000000
VERSION     DATGDI010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98132"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VKO45_04295"
     CDS             complement(<1..458)
                     /locus_tag="VKO45_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HMA05128.1"
                     /translation="MPGTMEFRLVAIEKPEDVNFILGQSHFIKTVEDIAEAVVGSVPG
                     AKFGLAFCESSGDCLVRAEGNDDNLKNLAVKNALDIGAGHSFILFLKDCFPINVLNAV
                     KGVQEVCSIYCATANPVEVIIAESATGRGILGVIDGAKPKGVEGPDEVAWR"
     gene            755..2650
                     /gene="acs"
                     /locus_tag="VKO45_04300"
     CDS             755..2650
                     /gene="acs"
                     /locus_tag="VKO45_04300"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HMA05129.1"
                     /translation="MVDQFDVKLVEEAKYYTPRPEYREKAWVRDYDEEYERFVKDPEG
                     FWAKVAKELDWYSPWKKVLDWQYPYAKWFVGAKLNITYNCLDRHVNNHRRNKVALIWK
                     GENGEERIYTYQMLYRDVNRFANGLKRLGIGKGDRVCIYMPMIPEQLIAMLGCARIGA
                     VHSVVFGGFGVTALNYRINDTESKVVITADIGFRRGKTIPLKTIVEEAIINAPSVERI
                     VVLRRKEPKVEIHSGIEIDFEDLMEGVEPYCKPEVMDAEDPLFVLYTSGSTGAPKGVI
                     HTCAGYMVGTYYTSKVVFDLKEDDIYWCTADVGWITGHSYILYGPLAAGATVFFGEVV
                     PDYPDAGAFWKLIQDTGITIFYTAPTAIRMFMKMGEDWPNKYDLSCLRILGSVGEPLN
                     PEAFEWYYRVIGKKNCPIVDTWWQTETGMQMITTFVGEKMRPGFAGKGIPGVIPDVVD
                     KAGRSLDPGKLGFLIIRNPWPAMFRTIYKNDERYRKYWETIPDVYTVGDLAVKGKDGY
                     IMILGRADDIIIVAGHNIGTAEVESALVSHQAVAEAAVIGKPDALKGNLIKAFVILKI
                     GQKATDKLKQDLKYHVRMTLGPIAQPEDIEFVDKLPKTRSGKIMRRVLKAKELGMDPG
                     DISTLEE"
BASE COUNT          638 a          866 c          936 g          520 t
ORIGIN      
        1 cgccaggcca cttcgtcggg cccctcgaca cccttcggct tcgccccgtc aataaccccg
       61 aggataccgc ggccagtagc agattccgcg atgataacct ccaccgggtt cgccgtcgcg
      121 cagtagatgc tacagacctc ctggacgccc ttcaccgcat tgaggacgtt gatggggaaa
      181 cagtctttca ggaagaggat gaaggagtgg cccgcgccaa tgtcgagggc gttttttacg
      241 gcgaggttct tcagattgtc gtcgttcccc tccgcccgga cgaggcaatc cccggatgac
      301 tcgcaaaagg cgaggccgaa cttcgcgccg ggcacggatc ccacgactgc ctccgcgata
      361 tcctcgacgg tcttgatgaa gtgggactgg ccgaggatga agttcacgtc ctcgggcttc
      421 tcgatcgcaa caagcctgaa ttccatggtt cctggcatgg gttcaccgga gagagatgaa
      481 tcctcgggat ttaaatccct tgactgcatg gggaagtatt gcacggcctt ttgcggaatg
      541 aatccttccc gccgccggag aggctcgcgc gatggcgggt cgaattcaat gccgcaaatt
      601 ctcgtggagg cctcctggat ccccctgata cggagtcggg gccttcggga ggacgtccga
      661 aaggatgacg gtaaacgacg gtgggccggc cgaaaatcag aaaattaata tagacattca
      721 tcatgattaa taatgtagaa taggggatgc cccgatggtg gatcagttcg acgtgaaact
      781 cgttgaggag gcgaagtact acacgcctcg ccccgagtac cgcgagaagg cctgggtcag
      841 ggattacgac gaggagtatg agaggttcgt caaggatccc gaggggttct gggcgaaggt
      901 ggcgaaggag ctcgactggt acagcccgtg gaagaaggtc ctcgactggc agtaccccta
      961 tgccaagtgg ttcgtcggtg ccaagctgaa catcacgtac aactgcctgg atcgccatgt
     1021 caacaaccac cggcggaaca aggtggctct catctggaag ggtgagaacg gcgaggaacg
     1081 gatctacacc taccagatgc tctaccggga tgtgaaccgg ttcgcaaacg gcctcaaaag
     1141 gctcggcatc ggaaagggcg accgggtctg catctacatg cccatgatcc ccgagcagct
     1201 catcgccatg ctcggctgcg cccggattgg cgccgtccac agcgtcgtct tcggcgggtt
     1261 cggcgtgacc gccctcaatt accggatcaa cgacaccgag tcgaaggtgg tcatcacggc
     1321 ggacatcggg ttccggcgcg ggaagaccat ccccctgaag accatcgtcg aggaggccat
     1381 catcaacgcc ccgagcgtgg agaggatcgt cgtcctccgg agaaaggagc cgaaggtgga
     1441 gatccactcc ggcatcgaga tcgacttcga ggatctcatg gagggggtgg agccgtactg
     1501 caagcccgag gtgatggacg ccgaggatcc cctcttcgtc ctctacacga gcggctcgac
     1561 gggggcgccc aagggggtca tccacacctg tgcggggtac atggtgggga cctactacac
     1621 gagcaaggtg gtctttgacc tgaaggagga tgacatctac tggtgcaccg ccgacgtggg
     1681 atggatcacc gggcactcct acatcctcta cggacccctc gccgccgggg ccacggtctt
     1741 cttcggcgag gtcgttcccg attaccccga cgccggggcc ttctggaagc tcatccagga
     1801 cacggggatc accatcttct acaccgcccc cacggccatc cgcatgttca tgaagatggg
     1861 ggaggactgg ccgaacaagt acgacctctc ctgcctccgg atcctcgggt cggtcgggga
     1921 acccctgaac cccgaggcct tcgagtggta ttaccgggtg ataggcaaga agaactgtcc
     1981 catcgtggat acctggtggc aaaccgagac gggcatgcag atgatcacga ccttcgtcgg
     2041 ggagaagatg cggcccggct ttgcgggaaa ggggatcccc ggggttatcc ccgacgtggt
     2101 ggacaaggcg ggaaggtccc tcgacccggg gaaactcggg ttcctcatta tcaggaaccc
     2161 ctggccggcc atgttccgga ccatctacaa gaacgacgag cggtaccgga agtactggga
     2221 gaccatcccg gatgtgtata ccgtcgggga cctcgcggtg aaagggaagg acgggtacat
     2281 catgatcctc gggagggccg atgacatcat catcgtcgcg ggccacaaca tcgggaccgc
     2341 ggaggtggag agtgcccttg tctcccacca ggccgtggca gaggcggcgg tcatcgggaa
     2401 gcccgacgcc ctcaagggca acctcatcaa ggccttcgtc atcctgaaga tcgggcagaa
     2461 ggcgacggac aagctgaagc aggacctgaa gtaccatgtc cggatgaccc tcggccccat
     2521 cgcccagccc gaggacatcg agttcgtgga caagctcccc aagactcggt cagggaagat
     2581 catgcgccgg gtcctcaagg ccaaggagct gggcatggac cccggcgaca tctccaccct
     2641 ggaggaatag gtcccgggcc cgcagggccg ggaccgtcca gggggatagg gaccctctgg
     2701 actaaacccc gggtccgaag gaatcccggg tccgaaagag ccgggattgt tcagggggga
     2761 taggggcccc tctgaactga gccgggaccg tccagggagg aaaaggcgcg aagcggcttc
     2821 tcctgtccag ggggggaggg gcccctctgg accaggggat ggatccctct gggcaaaccc
     2881 acttctttat ataccattta tctcctttta tataccatga aaggcagacc cgaggactcc
     2941 ctgaagatcg agaatatcgt
//
LOCUS       DATGDI010000113        13314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_417909, whole genome shotgun sequence.
ACCESSION   DATGDI010000113 DATGDI010000000
VERSION     DATGDI010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 13314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13314
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417909"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VKO45_04305"
     CDS             complement(<1..220)
                     /locus_tag="VKO45_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HMA05130.1"
                     /translation="MRILAIGLGGAGARIVDTLYGQDMQGSIHCMAAIAVDFDADTLQ
                     RLKHLPPAARIHFPPIDPTTPYDVQTTID"
     gene            570..1559
                     /locus_tag="VKO45_04310"
     CDS             570..1559
                     /locus_tag="VKO45_04310"
                     /EC_number="1.5.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydromethanopterin reductase"
                     /protein_id="HMA05131.1"
                     /translation="MSYGIEFVPGNVNVRNVVKFCKLAENKDIDFAWITNHYNNRHCY
                     PTLAMIAKETTTLQMGPGIMNSFTDTPAAMASFICTLDEISDGRAVLGIGPGDLSTLP
                     KIGIQGEKPVARLEEAIKVIRKLCAGEELKTPEMAFFKYQGAKLTGVNLPDKKKGVKV
                     YVGAQGPKVLDLAGRVGDGALINASNPKDFQVAIPIIRKATDAVGKKGFDIGAYTAMS
                     IDRDVKKARNAAKIVAAFIAAGSPPELLTRHGLNLDNVAKIKTALGKFDFKTVGELVS
                     DKEIDAFTIAGTPDMVKQKCDELTKAGVTQIIFGSPLGPDMTNSIRLLGKYVV"
     gene            1643..2575
                     /locus_tag="VKO45_04315"
     CDS             1643..2575
                     /locus_tag="VKO45_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HMA05132.1"
                     /translation="MFTERVVETGKGKLHLRRETLTGLSCRISSERSKRGIDRACSLE
                     FPTEGCPFCEANLAGATPTFPDGKRILVGESVTFPNLYPFAEWHTVTVISPDHSGSSL
                     TERQLEDAFSGQLASLLGRDGYPSINWNFLPSAGASLAHPHLQGLVDSRPAPLAARYL
                     AGSEEYLRKRERTYWDDLRRAERDAGRILKEGRIFWHAQAVPLGEKEIRGVLPVSHLP
                     GLRPLIPDLARDLLGFISFYRDLGTYAFNFALFFDREGCNGGFSAFCSLIARINPNPS
                     SMSDSAFMERLHLEPVILTLPENLARMFREWKER"
     gene            complement(2625..3467)
                     /locus_tag="VKO45_04320"
     CDS             complement(2625..3467)
                     /locus_tag="VKO45_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent methylenetetrahydromethanopterin
                     dehydrogenase"
                     /protein_id="HMA05133.1"
                     /translation="MVVKVGVAKLGNIASGVMAELLLDERADREDMQTFMATSGTKLQ
                     PEDIERVVSNMKAWKPDFCIVVSPNGVLPGPTGARESLLAAGIPCIIITDDITTKKEE
                     FEKIKASKFGYIIMKADAMIGARREFLDPIEMADYNGNLIKVLACTGAFRKLQNELDK
                     VIDQVKMGKKGDALVLPKVAISSDSAVKGEFNNPYALAKARAAYEIAQAVAGVNVKGC
                     FMTKEYVDYVPIVTSAHEMMRMAANLCDQARELEKGMDAVIRKPHKKDGVIVSKTKLV
                     SKPE"
     gene            3861..4265
                     /locus_tag="VKO45_04325"
     CDS             3861..4265
                     /locus_tag="VKO45_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfx family DNA-binding protein"
                     /protein_id="HMA05134.1"
                     /translation="MKDSLLTERQKEVLRYRKKGLTQQQIADIIGTSKANVCTIEKAA
                     LENIRRSKETLEFLYTLDATHLTTIKAGTDLLDAAKSVYGEAENLGIKVKYDTISLIN
                     QMRDSNPERFKARFIREDVEVYINDDGELYFG"
     gene            4921..5931
                     /gene="rpl3p"
                     /locus_tag="VKO45_04330"
     CDS             4921..5931
                     /gene="rpl3p"
                     /locus_tag="VKO45_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HMA05135.1"
                     /translation="MGKTRQPRKGSLAYSPRKRAKSQVPRYKSWQELEGAPFLQAFAG
                     YKVGMTHVVMVDDHKSSPTEGRDVVVPVTVVEVPALRVTAIRAYTEDSFGPRPLTEVW
                     SEALDPSLSRRIAVPKEHDRTAILAEMEKGIASGDIVDLRAVVQTQPGMVTGIPKKTP
                     ELLEIRIAGDNLAKRFEFAKSVLGKEVKAGDVVKAGQYVDVTAVTKGKGTQGPVKRWG
                     VKLRKRKHRVGRARIVGTLGPWHPHHIRWQVPMLGQTGYQQRTEPNKRILKIGDNGGE
                     VTPAGGFLHYGEVRNQYLLVKGSIPGPAKRLVRIRPAVRQGEHKFRVPVIQHVSTQSK
                     QG"
     gene            5939..6688
                     /gene="rpl4p"
                     /locus_tag="VKO45_04335"
     CDS             5939..6688
                     /gene="rpl4p"
                     /locus_tag="VKO45_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HMA05136.1"
                     /translation="MLAELKDLKGGVKKKVRLPPAFDEDFRPDLIKKAVLAIQSTRYQ
                     PHGAFPYAGIRTSAHSWGSGRGVAHVPRLKNGSRAARVPQAKGGREAHPPNAAKVLLE
                     KVNKKEKRKALLSAIAATTDEELVRGRGHQFEGKLPVVIEDAFEKLERTSDVIAVLQA
                     AGVYPDVERSRASRKVRPGRGKIRGRHYKQRKSLLIVTKGEPLRAARNLAGVDAVSVK
                     ELNAEHLAPGTHAGRLTVWTAGAVKQLGELS"
     gene            6685..6933
                     /locus_tag="VKO45_04340"
     CDS             6685..6933
                     /locus_tag="VKO45_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HMA05137.1"
                     /translation="MILKYPFVTEKAMMTLERDNKLQFLVENRATKPQIKREIEKTFE
                     KEVASVRTLMNTKGEKKAIITFVDEKAAEEILSRLGIM"
     gene            6943..7662
                     /locus_tag="VKO45_04345"
     CDS             6943..7662
                     /locus_tag="VKO45_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HMA05138.1"
                     /translation="MGHRIQTQNRGRGGPTYRAPSHLYRAEVHHAGRNDGLTRGKVLD
                     IEHDPARHGPVALVSLEDGRKTYVLATEGTGVGDSIAWGKGAEAKSGNTLPLSEIPVG
                     AMVSNIEAKPNDGGKFVRSGGTQALVMGRSDDNKVGVRMPSGKLKWFHPGCMATIGIV
                     SGGGRGEKPFVKAGKKYHKMQTGATKWPIVRGVAMNVIDHPFGGGSHQHAGRPKTIAR
                     GTSPGRTVGSVAARRTGIKKK"
     gene            7674..8087
                     /locus_tag="VKO45_04350"
     CDS             7674..8087
                     /locus_tag="VKO45_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HMA05139.1"
                     /translation="MVKKTQKRLPRRREEFTYRGFTLGELLQMAPSEQVKIMPARVRR
                     KFQRGLTRDEEDLLSRVRAGDPKIRTHLRSMVVMPEMVGKAIEIHNGKEFQKVDIQPE
                     AVYHYLGEFALTRHKVAHGAAGIGATRSSKYVPLK"
     gene            8093..8548
                     /locus_tag="VKO45_04355"
     CDS             8093..8548
                     /locus_tag="VKO45_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HMA05140.1"
                     /translation="MARTGYSMKLEGEDIARAKANEVDISPKHAFEIARFIKRMNTDD
                     AIEYLNRVVALEKAIPLRTYHKKVPHRHGLPGPGRYPQKAAKVFIQLLNAAKKNAEYI
                     GLDAGNTQIVHVQANRGRCHEAFFPRAMGRATPKNRETVNIEIIVKEVA"
     gene            8551..9258
                     /locus_tag="VKO45_04360"
     CDS             8551..9258
                     /locus_tag="VKO45_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HMA05141.1"
                     /translation="MAVERKFISEGVRRVRVEKYLTKELKRAGFGGMDIARTPLGTQV
                     TVFAEKPGIVIGKGGKVIRQLTLDLAANYGVDTPQVDVQQVPNPNFNAQIMAERLASA
                     IERGWYFRKAGSSIMRRVMDAGALGCEVIIAGKLTGPRARVQKFQEGYIKHAGEPSRT
                     VVERGYAIAIKKLGVIGVQVKIVPPDAKLPDSFVITKKVEAPPAEKATVTEVGEEMEP
                     EPAPEARDAFDRIEEGS"
     gene            9258..9461
                     /gene="rpmC"
                     /locus_tag="VKO45_04365"
     CDS             9258..9461
                     /gene="rpmC"
                     /locus_tag="VKO45_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HMA05142.1"
                     /translation="MAIFRAREVHQLSDVELQENMQKLRMELLQKYGKVSAGGAAENP
                     GRIREVRRTIARILTEQGTRKKA"
     gene            9458..9733
                     /locus_tag="VKO45_04370"
     CDS             9458..9733
                     /locus_tag="VKO45_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component 1"
                     /protein_id="HMA05143.1"
                     /translation="MIAAGNILRHELMGLPVEVVRSANPAQEGIAGRVVDETRNMLVI
                     GTPRGEKRIEKAHATFRLTLPDGTVADVSGIALVGQPEKRITQKKRF"
     gene            9743..10069
                     /locus_tag="VKO45_04375"
     CDS             9743..10069
                     /locus_tag="VKO45_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HMA05144.1"
                     /translation="MAKSVGLDVPAPATECGDVNCPFHGSLSVRGQVITGKVVSDRMK
                     GTVVVERDYLHYIRKYNRYEKRSSRIHAHNPPCIQAKAGDMVTIAECRPLSKTKSFVV
                     VEVEKP"
     gene            10066..10464
                     /locus_tag="VKO45_04380"
     CDS             10066..10464
                     /locus_tag="VKO45_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HMA05145.1"
                     /translation="MKAKQSYIPRALNTGSRLVCADNTGARMVQVISVDGYHGVRRRQ
                     PKLGLGDIATISVKKGTPDMRKKMEKAVVIRQKKEFRRPDGLRVSFEDNAMVIVNENN
                     EPKGTEIKGPVAREVAERFPKIGSMATIIV"
     gene            10473..10835
                     /gene="rplX"
                     /locus_tag="VKO45_04385"
     CDS             10473..10835
                     /gene="rplX"
                     /locus_tag="VKO45_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HMA05146.1"
                     /translation="MATTSSQPRKQRKAQANAPLHERGRTLSAPLAPALREQYKKRHL
                     RVVKGDTVKVTRGDHRGEEGLVDEVDVKRCILLVHGVSIPKADNTEVPRPVHPSKVQI
                     TKLNLKDRLRAERLGEVE"
     gene            10835..11566
                     /locus_tag="VKO45_04390"
     CDS             10835..11566
                     /locus_tag="VKO45_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HMA05147.1"
                     /translation="MAHLKRYAAPDSWHIRKKSTTYVAKTAPGPHDSGAMPVAVWLRD
                     HMSLARTMREVRQILNSGSVIINGRVCKNPSLGIGVFDIVSVPGIGKHYRILLDRRGR
                     YRSIEISPEDAKTRLAKIRGKTVISRGRVQLNLLFGANVIADNSYKPKDSVVLSLEEG
                     EGKKRFAILDHFPYAVGNVAMVIGGRHSGSVGRILEIRKEVGAVPNRVILENTATKEK
                     FDTIEEYVFMVGKAESAVKSWGIEE"
     gene            11563..12069
                     /locus_tag="VKO45_04395"
     CDS             11563..12069
                     /locus_tag="VKO45_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HMA05148.1"
                     /translation="MNPMQDLRIQKLVVHMGVGESGEKLSKAEEVLSTITHQKPVKAM
                     AKKTQPAFGTRKGSPIGCKVTLRSDAAEAFFETSIGIIDRQLPATHVDNQGNFSFGIE
                     EHTDFPGMSYDPSIGIFGMDVNVVLEKRGARISRRKVGQKKLPQKQRVKREEAIRFLQ
                     EKYKVEVR"
     gene            12071..12232
                     /locus_tag="VKO45_04400"
     CDS             12071..12232
                     /locus_tag="VKO45_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HMA05149.1"
                     /translation="MAVEQKEMKKFGRGAYQCLLCGRKQGLVRRYHIMFCRQCFREWA
                     QKMGFKKMN"
     gene            12247..12639
                     /locus_tag="VKO45_04405"
     CDS             12247..12639
                     /locus_tag="VKO45_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HMA05150.1"
                     /translation="MVRSNPISDAMTAIKNAADTGKADCIVEPAGKLLGSMLRIMQES
                     GYIGGFEFIDDNRGGQFRVQLNGRINRCGAINPRFNVKAEEMERWESQFLPGKNFGML
                     ILSTSRGVMAHDSARKLGIGGELLGYVF"
     gene            12651..13175
                     /locus_tag="VKO45_04410"
     CDS             12651..13175
                     /locus_tag="VKO45_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HMA05151.1"
                     /translation="MGATRKVKIPEGVTVRIEGNLVKVTGPKGMLMREMYYPNVSVSV
                     TDREFTITTESGRKKILAVCGTYAAHLQNMCRGVTEGYQYRMKVVYSHFPIQLKLAGD
                     RIEVGNFLGEKRPRFARVEKDVKVALGADEVTITGIDKESVGKTAANIEHATRIRERD
                     PRVFQDGVYVVERA"
BASE COUNT         2790 a         4055 c         4368 g         2101 t
ORIGIN      
        1 tgtcgatggt tgtctggacg tcgtaggggg tggtggggtc gatggggggg aagtggatac
       61 gggcggccgg cgggaggtgt tttaagcgct ggagggtatc ggcatcgaaa tcaacggcga
      121 tggccgccat gcagtggatg cttccctgca tgtcctgccc gtagagggta tctactatcc
      181 gcgcccctgc acccccgagg ccgatggcaa gtatccgcat ggccggttca gtccccgaaa
      241 agggaacgat cccgcatgct cgtatctagg agacagcggg ctaaatagtt cctcttccca
      301 tagatccgac ctcccggcgt ataaggttgg tggatgtctg atccgcccac gacggaagga
      361 cagggatcgg ccggattccc ggcatttccc tctccgccca gggcatacgg ggggagttca
      421 aacgggggaa ggggaggatt tcccgatgtc cccctgtatc ccgggaattc cccgaatcca
      481 ttgccgtaga ggcgcccgat tgagaaggcg gcacaaaatg ataatatata ttacctcttg
      541 tgtcggtata ctgtgctgag gtgaaagcct tgtcttacgg aatagaattc gtccccggaa
      601 acgtcaatgt caggaatgtc gtcaagttct gcaagcttgc cgagaacaag gacattgact
      661 ttgcatggat taccaaccac tacaacaacc gccactgcta ccccaccctc gccatgatcg
      721 cgaaggagac cacgaccctc cagatgggcc cgggtatcat gaactccttc accgacaccc
      781 cggcggccat ggcctccttc atctgcaccc tcgacgagat ctccgacggg cgtgcggtcc
      841 tcggtatcgg ccccggtgac ctctcgaccc tcccgaagat cgggatccag ggagagaagc
      901 cggttgcccg cctcgaagag gcgatcaagg tcatccggaa gctctgcgca ggcgaggagc
      961 tcaagacccc cgagatggcg ttcttcaagt accagggtgc caagctcacc ggcgtgaacc
     1021 tcccggacaa gaagaaggga gtcaaggtct acgtcggcgc ccagggcccg aaggttctgg
     1081 acctcgccgg cagggtcggc gacggggctc tcatcaacgc gtccaacccg aaggacttcc
     1141 aggttgccat ccccatcatc aggaaggcca ccgatgccgt cgggaagaag ggctttgaca
     1201 tcggtgccta caccgccatg tccatcgacc gcgacgtgaa gaaggcccgg aacgccgcga
     1261 agatcgtcgc agccttcatc gccgccggtt caccgcccga gctcctcacc cgccacggcc
     1321 tcaacctgga caacgtggcc aagatcaaga cagccctcgg caagttcgac ttcaagaccg
     1381 ttggcgagct cgtcagcgac aaggagatcg acgcctttac catcgccggg acccccgaca
     1441 tggtcaagca gaagtgcgat gagctgacca aggccggcgt cacccagatc atcttcggtt
     1501 ccccgctcgg gccggacatg acgaactcca tccggctcct cggcaagtac gtcgtctgag
     1561 gtcggaacag catcacccct ttttttcgcc gattatcccg ctggtttctt atagggaccc
     1621 gtcgttcgtt ctcttatcgc atatgttcac cgagcgggtc gtggagacgg ggaagggcaa
     1681 actccacctc cggcgggaga cgctcaccgg tctcagctgc aggataagct cggagcggag
     1741 caagaggggc atcgaccgag cctgttccct cgagttcccc accgaggggt gccccttctg
     1801 cgaggcgaac ctcgccgggg caaccccgac cttccccgac gggaagagga tcctcgtcgg
     1861 ggagagcgtc accttcccga acctctaccc cttcgccgag tggcacacgg tgaccgtgat
     1921 ctccccggac cacagcggct cctccctcac ggagaggcag ctcgaggatg ccttctcagg
     1981 gcagctcgca tcccttctgg gccgtgacgg ctatccatcc atcaactgga acttcctccc
     2041 ttcggcaggg gcgagcctcg cccaccccca cctccaggga cttgtggatt cccgcccggc
     2101 acccctcgcg gccaggtacc tggcagggag cgaggaatac ctccggaaac gggaaaggac
     2161 gtactgggac gacctccgga gggccgagcg cgacgcgggc cggatcctta aggagggaag
     2221 aattttctgg catgcccagg ccgtccccct cggcgagaag gagatccggg gggtcctgcc
     2281 cgtctctcac cttccggggc tcaggcccct catcccggac ctcgcccggg atctccttgg
     2341 tttcatctcg ttctaccggg acctgggcac ttacgccttc aatttcgccc tctttttcga
     2401 ccgggaaggg tgcaacgggg gcttctcggc cttctgctcc ctcatcgccc gtatcaatcc
     2461 caatccctcc tccatgagtg actcggcctt catggaacgc ctccacctcg agccggtgat
     2521 cctcacgctc ccggagaacc tggcgagaat gttccgggaa tggaaggaga ggtaatctcc
     2581 taaaatccgg gtaaccttgc agaaaaagag attgaaattt gtgtttactc gggcttgctg
     2641 acgagcttcg tctttgagac gatgacgccg tccttcttgt ggggcttccg gatgaccgcg
     2701 tccatgccct tctcgagctc acgggcctgg tcgcagaggt tcgccgccat gcgcatcatc
     2761 tcgtgggccg aggtgacgat ggggacgtag tccacgtact ccttcgtcat gaagcagccc
     2821 ttgacgttca cgccggcgac cgcctgggcg atctcgtagg cagcccgggc cttggcgagg
     2881 gcataggggt tgttgaactc gcccttcacg gcggagtccg aggagatggc gaccttgggg
     2941 aggacgaggg cgtcgccctt cttgcccatc tttacctggt cgatgacctt gtcgagctcg
     3001 ttctggagct tccggaaggc gccggtgcag gcgaggacct tgatcaggtt cccgttgtag
     3061 tcggccatct cgatggggtc gaggaactcc cgccgggccc cgatcatggc gtcggccttc
     3121 atgatgatgt agccgaactt ggaggccttg atcttctcga actcctcctt cttcgtcgtg
     3181 atatcatcgg tgatgatgat gcaggggatg cctgccgcaa ggagggactc ccgggcaccc
     3241 gtcgggccgg ggagaacccc gttcggggag acgacgatgc agaagtccgg cttccaggcc
     3301 ttcatgttgg agacgacacg ctcgatatcc tcgggctgga gcttcgtccc gctcgtggcc
     3361 atgaaggtct gcatgtcctc gcggtcagcg cgctcatcaa ggagcagttc tgccattaca
     3421 ccgcttgcaa tgttgccgag tttggcaact ccgactttta caaccatttt ttccacctct
     3481 gacgttggat aacatcgtag gtatcggtca aactacacat aaaacttgtg aaaccgcgtg
     3541 agaattccga ttccggcggg gagcccggat gcgttgccgg tgagagtctc gaaaccatcg
     3601 tgggcactcg ggtcatctgc cggacatatt acggattcgg aatgacaggt tccggatgat
     3661 ggcccgggga aatgtctgga tctgagtcca gggggtgaga tctcgattct gcaccgggga
     3721 agatacggaa aggtgacaag gctttgcggg gagcatccgg atctgcttcc tgggagggat
     3781 cggatcgccg cttcataagt ctccggagct gtcctgagtt aatcaaaagt gttaaatcgg
     3841 aagggaagaa ctatctcata atgaaagaca gtctcctcac cgaacggcag aaggaggtcc
     3901 tccggtaccg gaagaagggg ctgacccagc agcagatcgc cgatatcatc gggacatcga
     3961 aggcgaatgt ctgcaccatc gagaaggccg ccctcgaaaa catacggcgg tccaaggaaa
     4021 ccctcgagtt cctctatacc ctggacgcca cccacctcac gaccatcaag gccgggacgg
     4081 acctcctcga cgccgcgaag tccgtgtacg gcgaggccga gaacctcggg atcaaggtga
     4141 agtacgacac catttccctc atcaaccaga tgagggactc aaatccggag cgcttcaagg
     4201 cccggttcat ccgggaggac gtcgaggtct acatcaacga cgacggggaa ctctacttcg
     4261 ggtgagggag gttttgaagc gacgaagtcg cgagaaaccg agctggagca acccgccatc
     4321 tggcggggtg cgaccaggga ggttttgaaa acctgattgt tttctcatcc gctaccaccg
     4381 ccgtggacta tagcagcggc aggtgttcgt aagagggagg attcatcaga acccgacctg
     4441 gagcgacgaa gtcgcgagtc cttcccatct cacccccccc ttcgcggttc ccgggaggcc
     4501 ttcgtccata aatttatatc cctctccaat caattttata ggaagccgcc tgacaggcag
     4561 agggtatccc cgccttcaca ggaggttgaa cagaaccgag ttgtgccgac gggcacaacg
     4621 gctgacggaa gtcggatctg atgtcgccgc tcaagggcca acgtctctga acgtgccctg
     4681 taccggttaa ccggtacgcg gtgaacgatc aggcgctctt cgcgaaaatc tccgcgaaac
     4741 ttcgcgggac cgggcagtgc gcccgggcga accgccccat cccagagcca cttctcgccg
     4801 acccgaatgg ccggccatcc cccggaagct ccggcggggt ttctcagctg gaagagatca
     4861 aaagagtggt gttcgcgcga aagcctggtc tttcgcgcct ggaacaagga cggacagagt
     4921 atgggaaaaa caagacagcc gaggaagggt tccctggcct acagcccccg gaaacgggcg
     4981 aagagccagg ttccgcggta caagtcatgg caggagctcg aaggggcacc cttcctccag
     5041 gccttcgcgg gctacaaggt gggcatgacc cacgtggtca tggtcgatga ccacaagagc
     5101 agccccaccg aggggaggga cgtggtggtc ccggtcaccg tcgtggaggt cccggcactc
     5161 cgggtcacgg ccatccgcgc gtacacggag gacagcttcg ggccgaggcc cctcacagag
     5221 gtctggtcag aggccctgga cccctcgctc tcccgccgga tcgccgtgcc gaaggagcat
     5281 gaccggacgg ccatcctcgc tgagatggag aaggggatcg cctcgggtga catcgtcgac
     5341 ctcagggccg tcgtgcagac gcagcccggg atggtcacgg gcatccccaa gaagaccccc
     5401 gagctcctcg agatccggat cgccggggac aacctcgcga agcggttcga gttcgcgaag
     5461 tcggtcctcg ggaaagaggt caaggccggg gacgtggtga aggccggcca gtacgtggac
     5521 gtgacggccg ttaccaaggg gaaggggacc cagggtcccg tgaagcgctg gggcgtcaag
     5581 ctccggaagc ggaagcaccg ggtgggcagg gctcgtatcg tcggtaccct cggcccgtgg
     5641 cacccccacc acatccggtg gcaggtcccg atgctcgggc agaccggcta ccagcagcgg
     5701 accgagccga acaaacgcat cctcaagatc ggcgacaacg gaggggaggt caccccggcc
     5761 gggggcttcc tccactacgg cgaggttcgg aaccagtacc tcctcgtcaa gggatccatc
     5821 cccggcccgg cgaagaggct tgtccggatc cggcccgcgg tgaggcaggg cgaacacaag
     5881 ttccgggtgc cggtgatcca gcacgtgagc acccagagca agcaggggtg aggaaaggat
     5941 gctcgctgaa ctcaaggatc tgaagggcgg ggtaaaaaag aaggtccggc tcccgccggc
     6001 ctttgacgag gacttccggc ccgatctcat caagaaggcg gtcctcgcca tccagagcac
     6061 ccggtaccag ccccacgggg cattccccta cgcgggaatc cgcacctccg cccactcctg
     6121 ggggagcggt cgcggggtcg cccatgtgcc caggctgaag aacggttccc gggccgcccg
     6181 ggtcccccag gcaaagggtg gccgcgaggc ccacccgccg aacgcggcga aggtcctcct
     6241 cgagaaggtg aacaagaagg agaagaggaa ggccctcctc tctgccatcg ccgcgaccac
     6301 cgacgaggaa ctcgtccggg gccggggcca ccagttcgag ggcaagctcc ccgtcgtcat
     6361 cgaggatgcc ttcgagaagc tcgagcggac gagcgatgtc atcgcggtcc tccaggcagc
     6421 cggggtttat ccagacgtgg aacgctcccg ggcgagccgg aaggtccggc ccggccgggg
     6481 caagatccgc ggccggcact acaagcagcg gaagagcctc ctcatcgtga cgaagggcga
     6541 gcccctccgg gcggcgagga acctcgcggg ggtcgatgcc gtgagcgtga aggagctcaa
     6601 tgcggagcac ctcgcccccg gcacccacgc ggggcggctt accgtctgga ccgcgggggc
     6661 ggtcaagcag ctgggggagc tctcatgatc ctgaagtacc cctttgtgac ggaaaaggcg
     6721 atgatgaccc tggaacggga caacaagctc cagttcctcg tcgagaacag ggcgacgaaa
     6781 ccccagatca agagggagat cgagaagacc ttcgagaagg aggtcgcctc ggtccggacc
     6841 ctcatgaaca cgaaggggga gaaaaaggcc atcatcacct tcgtcgacga aaaagccgcc
     6901 gaggagatcc tctcccggct cggcatcatg taggtggaag agatgggaca cagaatccag
     6961 acacagaaca ggggccgggg cgggccgacc taccgggccc cctcccacct ctaccgcgcc
     7021 gaggtgcacc acgcggggag aaacgacggc ctcacccggg ggaaggtcct cgatatcgag
     7081 catgacccgg ccaggcacgg ccccgttgcc ctcgtctccc tcgaggacgg gaggaagacc
     7141 tacgtcctcg ccacagaggg cacgggcgtc ggggacagca tcgcctgggg aaagggtgct
     7201 gaagcgaaaa gcgggaacac ccttcccctc tcggagatcc cggtcggcgc catggtgagc
     7261 aacatcgagg cgaagcccaa tgacggcggg aagttcgtcc gctcgggcgg cacccaggct
     7321 ctcgtcatgg gcaggtccga cgacaacaag gtcggggtgc ggatgccctc cgggaaactc
     7381 aagtggttcc accccggctg catggcgacc atcgggattg tctccggcgg cgggaggggc
     7441 gagaagccct tcgtgaaggc cgggaagaag taccacaaga tgcagacagg ggccaccaag
     7501 tggcccatcg tccggggggt cgcgatgaac gtcatcgacc atcccttcgg cggtggctcg
     7561 caccagcatg cgggacgtcc gaagaccatc gcgaggggaa cctcgccggg aaggacggtc
     7621 ggctccgtgg ccgcgcggcg gaccgggatc aagaagaagt gaggtgaggg cacatggtga
     7681 aaaagactca gaaacggttg ccccggcggc gggaggagtt cacctaccgg gggttcaccc
     7741 tgggggagct cctccagatg gcgccctcgg agcaggtgaa gatcatgccg gcgcgggtga
     7801 gaaggaagtt ccagcgcggc ctcacccggg acgaggagga tctcctctcc cgggtccggg
     7861 ccggcgaccc gaagatccgc acccatctca ggtccatggt tgtcatgccc gagatggtgg
     7921 gaaaggccat cgagatccac aacggcaagg agttccagaa ggtggatatc cagccggagg
     7981 cggtgtacca ctacctcggg gagttcgccc tcacgagaca caaggtcgcc cacggcgccg
     8041 cgggtatcgg cgccacccgg tcgagcaagt atgtcccgct gaagtgatgg ccatggcgcg
     8101 aacaggatac agcatgaagc tcgagggcga ggacatcgcc cgggcgaagg cgaacgaggt
     8161 ggacatctcc ccgaagcatg ccttcgagat cgcccggttc atcaagagga tgaatacgga
     8221 cgatgccatc gagtatctca accgggtggt ggccctcgag aaggccatcc ccctccggac
     8281 ctaccacaag aaggtccccc accgccacgg ccttccgggc ccgggaaggt acccccagaa
     8341 ggcggcgaag gtcttcatcc agctcctcaa cgcggcaaag aagaacgccg agtacatcgg
     8401 ccttgacgcg ggaaacacgc agatcgtcca tgtgcaggcc aaccggggca ggtgccacga
     8461 ggcctttttc ccaagggcca tggggcgtgc gacgccgaag aaccgggaga ccgtgaacat
     8521 cgagatcatc gtgaaggagg tggcctaggc atggccgtcg agagaaagtt catctccgag
     8581 ggagtccgcc gggtccgggt cgagaagtac ctgaccaagg agctgaagag ggccggcttc
     8641 gggggcatgg acatcgcccg gacgcccctc gggacccagg tgacggtctt tgccgagaag
     8701 ccggggatcg tcatcgggaa gggcggcaag gtcatccggc agctcaccct ggatcttgcg
     8761 gcaaactacg gggtcgacac cccgcaggta gatgtccagc aggtcccgaa ccccaacttc
     8821 aatgcccaga tcatggccga acggctggcc tccgccatcg agcgcggctg gtacttccgg
     8881 aaggccggat cgagcatcat gcggagggtg atggacgcgg gcgccctggg ctgcgaggtc
     8941 atcatcgccg ggaagctgac cgggccgagg gcccgggtcc agaagttcca ggaagggtac
     9001 atcaagcatg ccggtgaacc gagcagaacg gtggtggaac gcgggtacgc catcgccatc
     9061 aagaagctcg gggttatcgg ggtgcaggtg aagatcgtgc ccccggacgc gaagcttccc
     9121 gacagtttcg tcatcacgaa gaaggtggag gccccgccgg ccgagaaggc aacggtcacc
     9181 gaggtcggcg aggagatgga gcccgagccc gcgcccgagg cgcgggacgc tttcgaccgg
     9241 atcgaggagg gctcctgatg gccatcttcc gggcccggga ggtccaccag ctctccgatg
     9301 tggagctgca ggagaacatg cagaagctcc ggatggagct tctccagaag tacggcaagg
     9361 tgagcgccgg cggcgccgcc gagaatcccg ggcggatccg ggaggtccgg cggaccatcg
     9421 cccggatcct caccgagcag ggcacgagga agaaggcatg atcgcggccg ggaacatcct
     9481 ccgccacgag ctcatgggcc tccccgtgga ggtcgtgcga tcggcaaacc ccgcccagga
     9541 agggatcgcc gggcgcgtcg tggacgagac aaggaacatg ctcgtgatcg ggaccccccg
     9601 gggagaaaag aggatcgaga aggcccacgc gaccttccgg ctcactctcc ccgacgggac
     9661 ggtggcggat gtttccggga ttgccctcgt cggccagcct gagaagagaa ttacacagaa
     9721 aaagaggttc tagaggaata ccatggcaaa gagtgtaggt ctggatgtcc ctgccccggc
     9781 gacggaatgc ggggacgtca actgcccgtt tcacggcagc ttgtcggtgc gcggccaggt
     9841 gatcaccggc aaggtcgtga gcgaccggat gaagggaacg gtggtggtgg agagggatta
     9901 cctccactac atccggaaat acaaccgata cgagaagcgg agctcgagga tccatgccca
     9961 taacccgccc tgcatccagg caaaggcggg ggacatggtg accatcgccg agtgccggcc
    10021 cctctcgaag accaagtcct tcgttgtcgt cgaggtggag aagccatgaa ggcgaagcag
    10081 tcctatatcc cgagggcgtt aaacaccggc tcacgcctcg tctgcgccga taacaccggc
    10141 gcgaggatgg tccaggtcat ctcggtggac ggctaccacg gcgtgcggag gaggcagccg
    10201 aagctcgggc tcggcgacat cgccaccatc tcggtgaaga agggaacgcc ggacatgagg
    10261 aagaagatgg agaaggccgt cgtgatccgg cagaagaagg agttccggcg gccggacggc
    10321 ctccgggtct ccttcgagga caatgcgatg gtcatcgtga acgagaacaa cgagccgaag
    10381 gggacggaga tcaagggccc ggtcgcccgc gaggtggccg agcggttccc gaagatcggc
    10441 tccatggcca ccatcatcgt gtgaggcgcg aaatggcaac gacaagttca caaccgagaa
    10501 agcagaggaa ggcccaggcg aacgcgcccc tccacgagcg gggcaggacc ctctcggcac
    10561 ccctcgcacc cgcactcagg gagcagtaca agaaaaggca cctccgggtc gtgaagggcg
    10621 acacggtgaa ggtcacgaga ggcgaccacc ggggcgagga aggcctcgtc gacgaggtgg
    10681 acgtgaagcg ctgcatcctc ctcgtccacg gggtttccat cccgaaggcg gataacaccg
    10741 aggtcccgag gcccgtccac ccctccaagg tccagatcac gaagcttaac ctgaaggacc
    10801 ggctccgggc cgagcggctc ggggaggtgg agtgatggcg cacctgaagc ggtacgcggc
    10861 ccccgactcc tggcacatcc gcaagaaatc caccacctac gtggcgaaga ccgctcccgg
    10921 cccgcacgac agcggggcca tgcccgtggc cgtctggctc cgggaccaca tgtccctcgc
    10981 ccggacgatg cgggaggtca ggcagatcct gaactccgga tcggtcatca tcaatggaag
    11041 ggtctgcaag aacccgagcc tgggaatcgg ggtctttgac atcgtgagcg tgcccggcat
    11101 cgggaagcac taccggatcc tcctggacag gcggggccgg taccggtcca tcgagatctc
    11161 gcccgaggac gcgaagacac ggctcgcgaa gatccggggc aagaccgtga tctccagagg
    11221 gagggtccag ctgaacctcc tcttcggggc gaatgtcatc gccgacaaca gctacaagcc
    11281 caaggactcg gtggtcctct ccctcgagga gggcgagggg aagaagcgct tcgccatcct
    11341 cgaccacttc ccgtacgccg tcgggaacgt ggccatggtc atcggcggga ggcactcggg
    11401 ctccgtcggc cggatcctcg agatccggaa ggaggtcggg gccgtcccga accgggtcat
    11461 cctcgagaat accgccacga aggagaagtt cgacaccatc gaggagtacg tcttcatggt
    11521 gggaaaggcg gagtctgccg tgaagtcctg ggggattgag gaatgaaccc gatgcaggat
    11581 ctccggatcc agaagctcgt cgtccacatg ggcgtcgggg agagcggcga gaagctctcc
    11641 aaggcggagg aggtgctctc gaccatcacc caccagaagc cggtgaaggc catggcaaag
    11701 aagacccagc cggccttcgg gacccggaag ggttccccca tcgggtgcaa ggttaccctc
    11761 cggagcgatg ccgccgaggc cttcttcgag acctccatag gtatcatcga ccggcagctc
    11821 ccggccaccc acgtcgacaa ccagggcaac ttctccttcg ggatcgagga acacacggac
    11881 ttcccgggga tgagctacga cccgtccatc gggatcttcg ggatggacgt gaatgtcgtc
    11941 ctcgagaaga ggggcgcccg gatctcccgg cggaaggtcg ggcagaagaa gctcccccag
    12001 aagcagcgcg tgaagcggga ggaggccatc cggttcctcc aggagaagta caaggtggag
    12061 gtgcggtgac atggccgttg aacagaagga gatgaagaag ttcgggaggg gggcgtacca
    12121 gtgcctcctc tgcgggcgga aacagggcct cgtgcggagg taccatatca tgttctgccg
    12181 gcagtgcttc cgggaatggg cccagaagat gggcttcaag aagatgaact aggtggtgaa
    12241 acgaccatgg tcagatcaaa tcccatctcg gacgcgatga ccgccatcaa gaacgccgcc
    12301 gataccggga aggccgactg catcgtcgag cctgccggga agctcctggg ttcgatgctc
    12361 cgcatcatgc aggagtcggg gtacatcggg gggttcgagt tcatcgacga taaccggggc
    12421 ggccagttcc gggtccagct gaacggccgg atcaaccgct gcggggccat caacccgagg
    12481 ttcaatgtga aggcagagga gatggagcgc tgggagagcc agttcctccc ggggaagaac
    12541 ttcgggatgc tcatcctctc cacgagcagg ggcgtcatgg cccatgacag cgcccggaag
    12601 ctcgggatcg gcggggaact cctggggtac gtgttctaga ggtgacggaa atgggagcga
    12661 caagaaaggt gaagatcccg gagggcgtga cggtgcgtat cgagggaaac ctcgtcaagg
    12721 tcaccggccc gaaggggatg ctcatgcggg agatgtacta cccgaacgtc tccgtctcgg
    12781 tcaccgatcg ggagtttacc atcaccaccg aatccgggag gaagaagatc ctcgccgtct
    12841 gcgggaccta cgcggcccac ctgcagaaca tgtgcagggg cgtgacggag gggtaccagt
    12901 accggatgaa ggtggtgtac agccacttcc ccatccagct gaagctcgcc ggtgacagga
    12961 tcgaggtggg caacttcctc ggtgagaagc ggccccggtt cgcccgggtc gagaaggacg
    13021 tgaaggtggc cctcggggcg gacgaggtga ccatcacggg catcgacaag gagtcggtgg
    13081 gaaagaccgc cgcgaacatc gagcacgcga cccggatccg ggagagggac ccccgcgtct
    13141 tccaggacgg ggtctacgtc gtggagaggg cgtgatccgt atggcagacg agaaggcaag
    13201 gctcatccgg gtgcggaacg ggaaccggcc acggttccag cggcggggta tccacgagaa
    13261 gaagcgcctc gccgacgtgt ggcgcaagcc gaagggccgg cacaacaagc tccg
//
LOCUS       DATGDI010000114         8797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_98423, whole genome shotgun sequence.
ACCESSION   DATGDI010000114 DATGDI010000000
VERSION     DATGDI010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98423"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..979
                     /locus_tag="VKO45_04415"
     CDS             41..979
                     /locus_tag="VKO45_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMA05152.1"
                     /translation="MEAVVTGGCGFIGSHLVNALVGRGEKVTVIDDLSSGSEANIAGL
                     LRTRRATLRRADLLSEGWEDALEGVPVVYHLAADPDVRASATRPEEVIGRNILATGRV
                     LEAMRRKDVPRIVFTSTSTVYGEASVIPTPEDYAPLEPISVYAASKLACEALISAYCH
                     TYGMQGWVFRFANIIGERSNHGVIGDFIRKLRENPQSLEILGDGRQAKSYLEVGECVR
                     AMLHAVDGAGGVYNVYNIGSEDWIDVKGIADIVVATLGLTGVEYRFTGGDRGWVGDVP
                     RMLLSVERLKGLGWRPALGSRESVVAAARALAGDRR"
     gene            976..2265
                     /locus_tag="VKO45_04420"
     CDS             976..2265
                     /locus_tag="VKO45_04420"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HMA05153.1"
                     /translation="MKVLLVLGDGMADEPIPGLGGRTPLEAARTPNFDRIARDGACGM
                     VRTIPPGFEAGSDIANLSLLGYDPRRYYTGRGPLEAASMGVTLGPGDIAYRCNLVTVT
                     DGVMEDFSAGHIPSDEGAPLIQAVAAGVEGVSLRAGISYRNLMVIPKGGGAVTTPPHD
                     IVGERIEPHLPEGRDAPLLLRVMERSREILRDHPVNRDRIARGQRPATMIWPWSGGKA
                     PDLPSFRELHGVTGAMISAVDLLNGIARIAGMEVIRVPGATGYLDTDYDAKARYAIQA
                     LERFDFLYLHVEAPDEAGHLGSVEEKVKAIERVDGMVGTIMEGFEGIIAVLPDHPTPI
                     RLKTHTADPVPFAVRGATCDVSIGKRSKSTTKWCEFPPRGTEQEHRETVRLRRDGCNR
                     LTEREAARGGLGLLEAGSFLPMLLLRRPRTGEARDRT"
     gene            2262..3182
                     /locus_tag="VKO45_04425"
     CDS             2262..3182
                     /locus_tag="VKO45_04425"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMA05154.1"
                     /translation="MILVTGSGGLLGANFVLTALHRQREVAATFHRNAIRIPGVSALP
                     LDITDRDSVERVIASLEPEWIVHCAAVTNVDWCEDHPAECHSVNEEATRTLARAARDH
                     GSSMTYISTDAVYRGSDGNYDEGSPADPVNVYGRSKLAGENAVREVLGDPLILRTNIF
                     GWNLQPKHSIAEWFLSGLEAGKRVPGFTDVLFSPLLVNSLSEIILDLMDARKTGTFNA
                     GCVRPCSKYEFGRMLAETFHLDPGWVQPVRLADTHLRAPRPLDTSLDTRKISHALGRG
                     MPDVGSQLARFKYLRDSGFAGELRDSWGGS"
     gene            3186..4259
                     /locus_tag="VKO45_04430"
     CDS             3186..4259
                     /locus_tag="VKO45_04430"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HMA05155.1"
                     /translation="MIIETAKKRIGDGQPVYFVADIAANHEGDLGRALELIRLAAGAG
                     ADAAKFQNFRAPEIVSEYGFEHLGGGRSHQAHWKRPVYRVYEDASLPWEWTPVLRAEC
                     DRVGIDFFSTPYDLEAVEMLDPYVSLFKIGSGDIDWMEMLTAVAARGKPVILATGASE
                     MREVTRAVKHILAINPALVLLQCNTNYTVSRENFRHINLSVLRTYLAQFPGVICGLSD
                     HTKGHAAALGAVALGGKVIEKHFTDDDTRDGPDHPFSMTPAEWREMVDRTRELELALG
                     NGEKVVEENERETLVVQRRCIRAARPLPAGEVIRREDLKILRPAPPGSIKPSDLDRVI
                     GLRVRIPVPEGDYLRWAGLEPRT"
     gene            4264..5391
                     /locus_tag="VKO45_04435"
     CDS             4264..5391
                     /locus_tag="VKO45_04435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMA05156.1"
                     /translation="MRILFFSRGFSPHERRFLEKMAGWRHEISFLMLENPGEFRPEER
                     LPDGIAFAGSLGFTGGVIPRDEQPGLVARFQEVCDRARPDLVHAGPIQSCGFLSALAG
                     FHPLLLMSWGSDILRDADTDDLSRRVTRYALRASDWLFCDCVAVRNRVKEFTPYEDAR
                     MVMFPWGVDLRRFRPSPRPSSLRRDLGWEDRTVLLSTRMWEPVYGINTLLEAFQIASA
                     KHPDLRLILLGKGSLGSRVGGFIREHDLDDRVAMPGMVDEPEMPAYFHASDLYISCSL
                     SDGTSVSLLQAMACGLPVVVTDIPGNREWVTEGENGLLARGGDPRAYARAISRTLEIP
                     AAEGEGIARMNRRIVEERADWDKNILLLLATYDRIEEVYGP"
     gene            5381..6397
                     /locus_tag="VKO45_04440"
     CDS             5381..6397
                     /locus_tag="VKO45_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMA05157.1"
                     /translation="MGHKSLSREKARILVIGCGSIGKRHIRNLQVAGAREITAFDPRE
                     DRRREAGEGLGIRVFADLEAAFAAGPDAVFVCSPTAFHREQSLRAVAAGSHVFIEKPV
                     SDSLEGMDTLMEVVDRQGLVSLVGCNFRFHPGLRQVKSLLNSGSIGRTISFRARFGHY
                     LPDWHPWEDYRKTYSARRDLGGGVILDRVHEIDYARWLFGDVRQVFSWAGHESSLEID
                     TEDTAEILLGFRNGITGSLHLDYLRRRYDAGLEVTGEEGIIQWSYQNHTVTWYEAGKG
                     SWQSMRWPGYDGNKMYLDEVMHFLAALEGGEQSVNPVPDAVRVLEIALAAKRSSTDGC
                     VVNL"
     gene            complement(6486..7808)
                     /locus_tag="VKO45_04445"
     CDS             complement(6486..7808)
                     /locus_tag="VKO45_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMA05158.1"
                     /translation="MKREKNSGLTLWQRAKRIIPGGSQLLSKRSEQFLPDLWPSYYRS
                     AKGVEVTDLDGRRYIDMSLLGVGACPLGYADPDVDRAVKEAIDQGSMCTLNCPEEVEL
                     AEVLLRLHPWAEMVRFARGGGEAMAVAVRIARARTGREIVAFCGYHGWHDWYLSANLA
                     DDRNLDGHLLPGLEPRGVPRGLKGTAVPFRYNEIEDLRAIIDRHDGSLAAVVMEPIRD
                     HEPVPGFLEEVQELAAAAGALLVVDEVSAGFRLNTGGAHLVYGIRPDIAVFAKGMSNG
                     YPMAAIIGRGDAMSAAQETFISSTYWTERIGPVAALATIDKYGRCNVPEHLVRTGKQV
                     QGLWARAAADAGLPLVVEGIPPLSHFTFSRPEALEMQTAFTQMMLDRGFLAGKAFYSS
                     FAHKGTHIEKYRNAVDEVFLDLASALKKGEMAGLLKGPVAHAGFGRLT"
     gene            complement(7805..8587)
                     /locus_tag="VKO45_04450"
     CDS             complement(7805..8587)
                     /locus_tag="VKO45_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HMA05159.1"
                     /translation="MSAVAIIQARMGSTRLPGKVLMDLCGKPVLWHVVQRTQAAQRVH
                     RVLVATTRRAEDDAIKELCREWGTPVYRGPAEDVLARYHGAVRYLDRQGVPVDLIVRI
                     TADCPLMDPAVIDRGVREAEEKNPDYISNVDPPTFPDGLDVEVFTRQVLEITHSEARL
                     PSDREHVTPFMRRSGRFKTVNFRAVTDLSHLRWTLDTREDYLFIREVYRHLFPQKNLF
                     ALEDVVRLLADHPELSGINSHLKRNEGYRLSLEKDTRILGAE"
BASE COUNT         1617 a         2786 c         2830 g         1564 t
ORIGIN      
        1 gaacaccggt attttacatg gggagggcaa ataagctctc atggaagcgg tggtcaccgg
       61 gggctgcggg ttcatcggct cgcatctggt gaacgccctc gtcggccggg gggagaaggt
      121 caccgtgatc gatgacctga gctccggctc cgaggcgaat atcgcgggtc tcctcaggac
      181 gaggagggcc accctccgga gggccgacct cctctcggag gggtgggagg atgccctcga
      241 gggggtcccg gttgtctacc acctcgccgc cgaccccgac gtccgggcga gtgcaacccg
      301 ccccgaagag gtgatcggga ggaacatcct cgccaccggc cgggtcctcg aggcgatgcg
      361 ccggaaggac gttccccgca tcgtcttcac ctccacctcg accgtctacg gcgaggcatc
      421 ggtcatcccc acccccgagg actacgcccc cctcgagccc atctcggtgt atgcggcgag
      481 caagctcgcc tgcgaggccc tgatctcggc gtactgccac acgtacggta tgcagggatg
      541 ggtcttccgg ttcgcgaaca tcatcggcga gcgatcgaac cacggggtca tcggggactt
      601 catccggaag cttcgggaga acccccaaag cctcgagatc ctcggggacg ggaggcaggc
      661 caagtcctac ctggaggtgg gggagtgcgt gagggcgatg ctccatgccg tggacggggc
      721 agggggggtg tacaacgtct acaacatcgg ctccgaggac tggatcgacg tgaaggggat
      781 cgccgacatc gtggtcgcca ccctcggtct caccggcgtg gagtaccggt tcaccggtgg
      841 cgaccggggg tgggtgggag acgttccccg gatgctcctc tccgtcgaac ggctgaaggg
      901 gctcggctgg agaccggccc tgggctcgag ggagagcgtc gttgcagcgg cccgggccct
      961 ggcgggtgac cggcggtgaa ggtcctcctc gtcctcggcg acgggatggc cgacgagccc
     1021 atccccgggc tcggcgggag aacccccctc gaggccgccc ggaccccgaa cttcgaccgg
     1081 atcgcgaggg acggtgcctg cggcatggtc aggaccattc ctcccggctt cgaggcgggg
     1141 agcgatatcg cgaacctctc cctcctggga tacgatcccc ggaggtacta caccggccgt
     1201 ggccccctcg aggcagcgag catgggagta accctgggcc ccggggacat cgcctaccgg
     1261 tgcaacctgg tgaccgtgac agacggggtg atggaggact tctcggccgg ccacatcccg
     1321 agtgacgagg gtgcccccct tatccaggcc gtcgcggcag gggtcgaagg ggtttccctc
     1381 cgtgccggta tctcgtaccg gaacctcatg gtcatcccga aaggaggggg tgccgtcacg
     1441 accccgcccc acgacatcgt cggggagagg attgaacctc atctaccgga gggcagggac
     1501 gccccgctcc tcctcagggt catggagaga tcccgggaga tcctccggga tcatccggtg
     1561 aaccgggatc gcatcgcacg ggggcagcgt ccggcgacca tgatctggcc ttggagcggt
     1621 ggaaaggcac ccgatctccc ctccttccgg gagcttcacg gcgtcaccgg cgcgatgatc
     1681 tccgcagtgg atctcctgaa cgggatcgcc cgtatcgcgg ggatggaggt gatccgggtg
     1741 cccggcgcga cagggtacct cgacacagac tatgatgcaa aggcgcggta cgcgatacaa
     1801 gcgctggaac ggttcgactt tctttacctc cacgtcgagg cacccgacga ggccggccac
     1861 ctgggctcgg tggaggagaa ggtgaaggcc atcgagcggg tcgacgggat ggtcgggacc
     1921 atcatggagg gattcgaggg aatcatcgcc gttctccccg accaccccac ccctatcagg
     1981 ctgaagaccc acaccgccga cccggtgcct tttgcggtgc ggggtgcgac gtgcgacgtt
     2041 tccatcggga aacggagcaa gagcaccacg aagtggtgcg agttccctcc taggggaacg
     2101 gagcaggagc accgggaaac ggtgcgactc cgcagggacg gatgcaaccg gctcaccgag
     2161 cgggaagcgg cgagaggggg tttaggtctg ctggaggccg gctcgttcct gccgatgctc
     2221 cttttgcggc ggccacggac cggagaggcc agggaccgaa catgatcctc gtgaccggat
     2281 cgggcggcct cctcggggca aacttcgtcc tcaccgccct ccacaggcag cgggaggtcg
     2341 ccgccacctt ccacaggaat gccatccgga tccctggcgt gagtgcgtta cccctggata
     2401 tcacggatcg cgattcggtg gagagggtca ttgcgtccct tgaaccagaa tggattgtcc
     2461 actgtgccgc cgtcacgaac gtggactggt gcgaggatca tccggcggaa tgtcactcgg
     2521 tgaacgagga ggcgacccgg accctggcac gtgccgcccg ggaccatggc tcctcgatga
     2581 catacatctc cacggatgcg gtctaccggg gcagcgacgg gaattacgac gagggttccc
     2641 ccgccgaccc ggtgaacgtg tacgggcggt cgaagctcgc aggggagaac gcggtgaggg
     2701 aggtgctcgg ggatcccctg atcctccgta ccaatatctt cggatggaac ctccagccga
     2761 aacactccat cgcagaatgg ttcctctccg gactcgaggc cggaaagcgc gttcccgggt
     2821 ttacggatgt cctgttctcc cccctgctcg tgaattccct gtccgagatt atcctcgatc
     2881 tcatggatgc ccgaaagacc gggaccttta atgccggatg cgttcgcccc tgcagcaagt
     2941 acgaattcgg gaggatgctc gcagagacct tccacctcga ccccgggtgg gtccagcccg
     3001 tgcgtctcgc cgacacccac cttcgcgcgc cccgcccgct cgacacctcc ctggacaccc
     3061 ggaagatctc gcatgccctg gggaggggga tgcccgacgt gggatcgcag ctcgcccggt
     3121 tcaaatacct cagggattcg ggatttgcgg gagaactcag ggattcctgg ggaggttctt
     3181 gacccatgat catcgaaaca gcgaaaaagc ggatcgggga tggccagcct gtatacttcg
     3241 tcgccgatat tgcggcgaac catgaggggg atctcggccg ggcactggag ctgatccggc
     3301 ttgcggcagg ggcgggagcg gatgccgcga agttccagaa cttccgggca ccggagatcg
     3361 tgagcgagta cggcttcgaa cacctgggcg gggggcgttc gcaccaggct cattggaaga
     3421 ggccggtcta tcgcgtttat gaagacgcct ccctgccctg ggaatggacc ccggtgcttc
     3481 gggccgagtg cgaccgcgtt gggatcgact tcttctccac cccctatgac ctggaggccg
     3541 ttgagatgct cgacccgtat gtcagcctct tcaaaatcgg ctcgggggac atcgactgga
     3601 tggagatgct cacggcggta gccgcgaggg ggaagccggt catcctcgcg accggcgcgt
     3661 cggagatgcg ggaggtcacc cgggccgtca agcacatcct cgccatcaac cctgccctgg
     3721 tgcttctcca gtgcaacacg aactacacgg tctcccggga gaatttcagg cacatcaacc
     3781 tgagcgtcct caggacctac ctggcccagt tcccgggggt catctgcggt ctctcggacc
     3841 acacgaaggg ccatgcggcc gccctcgggg cggtcgccct cggaggcaaa gtcatcgaga
     3901 agcacttcac cgacgacgac acccgggacg gccccgacca ccccttctcg atgacccccg
     3961 ccgagtggag ggagatggtg gacaggaccc gggagctgga gctggccctg gggaacggag
     4021 agaaggtggt ggaggagaac gagcgggaaa ccctcgtcgt gcagaggaga tgcatccggg
     4081 ccgcccgccc cctgcccgcg ggcgaggtca ttcggagaga ggacctgaag atcctcaggc
     4141 cggccccccc gggctccatc aagccctcgg acctggaccg ggtcatcggc ctgcgggtcc
     4201 gcatccccgt ccccgaagga gactacctcc gctgggccgg gctcgaaccc agaacgtgat
     4261 tccatgagga tcctgttctt cagcagggga ttttccccgc acgagcgccg gttcctggag
     4321 aagatggcag gctggcgaca tgaaatctcc ttcctcatgc tggagaatcc cggggagttt
     4381 cgcccggagg agaggcttcc cgatggaatt gcctttgcag ggtcgctcgg attcaccgga
     4441 ggggtgatac cccgtgacga gcagccaggg ctcgtggccc ggtttcagga agtgtgcgac
     4501 cgggcgcgac cggatctcgt ccatgcggga cccatccagt catgcggttt tctctcggcc
     4561 ctggccgggt tccatcccct gctcctcatg tcatggggct cggacatcct ccgggatgcc
     4621 gacaccgatg atctctcgag acgggtgacc cggtacgccc tccgcgcctc cgactggctt
     4681 ttctgcgact gcgtggcggt caggaaccgg gtgaaggagt tcacccccta cgaggatgcg
     4741 aggatggtca tgttcccctg gggcgtagac ctccggaggt tccggccctc cccgcggcct
     4801 tcatccctcc gcagggatct gggctgggag gaccggaccg tgctcctctc gacgcgcatg
     4861 tgggaaccgg tgtacgggat caataccctc cttgaggcct tccagatcgc cagtgcaaag
     4921 catcccgatc tcaggctgat cctcctcggc aaaggctcac ttggatcccg ggtcgggggc
     4981 ttcatcaggg agcatgacct ggatgaccgg gtggccatgc ccggcatggt ggacgagccg
     5041 gagatgcccg catacttcca tgcctctgac ctgtacatct cctgctcgct ttccgatggc
     5101 acctcggtat cactcctcca ggcgatggcc tgcggcctcc ctgtcgtggt gaccgacatc
     5161 ccggggaaca gggaatgggt gacagagggg gagaacgggc tccttgcgag gggaggggat
     5221 ccccgggcct atgccagggc aatctcccgg accctggaga tcccggccgc tgaaggggag
     5281 gggatcgccc ggatgaaccg caggatcgtg gaagagaggg cggactggga taaaaacata
     5341 ttattgctcc tcgccaccta tgatcgaatc gaggaggtat atgggccata aatccctttc
     5401 cagagagaag gcacggatcc ttgtcatcgg ttgcgggtca atagggaaaa gacacatacg
     5461 caacctccag gtggccggcg cgagggagat caccgccttt gatcccaggg aggaccggcg
     5521 ccgggaagcg ggcgaaggcc tcgggatacg ggtgttcgcc gacctcgagg ctgcctttgc
     5581 cgcagggccc gacgccgtgt tcgtctgctc ccccaccgcc tttcaccggg agcaatccct
     5641 ccgggccgtg gccgcgggct cccatgtctt catcgagaaa ccagtctctg actccctgga
     5701 agggatggat acactcatgg aggtagtgga caggcagggc ctcgtctccc tggtgggatg
     5761 caacttcagg ttccatccgg ggctgcggca ggtgaagtcc ctcctcaaca gcgggagtat
     5821 cgggaggacc atctccttcc gggcccgctt cgggcattac ctgcccgatt ggcacccctg
     5881 ggaggactat cgcaaaacct acagtgcacg gagggatctc ggcggggggg tcatccttga
     5941 ccgggtccat gagatcgact atgcacgatg gctcttcggg gacgtgcgtc aggttttttc
     6001 ctgggccgga catgaaagca gccttgagat agacaccgag gacacggccg agatattact
     6061 cgggttcagg aacgggatta cgggatcgct ccacctggat tacctcagga gacggtacga
     6121 tgcaggcctc gaggtaacgg gggaggaggg catcatccag tggtcatacc agaaccacac
     6181 cgtcacctgg tacgaagccg ggaagggatc atggcaatcc atgaggtggc cggggtacga
     6241 cgggaacaag atgtacctcg acgaggtcat gcacttcctt gcggccctcg aaggagggga
     6301 acaatccgtc aacccggtcc ccgatgcggt acgggtgctc gagatcgccc tcgcagcaaa
     6361 gcgatccagt accgacgggt gcgtcgtgaa cctgtgatgt gtgtttcctt ctcccgggcc
     6421 acccccgaga ggaccatgga ggcatgcttc taccgggaat cgctttctgc aaagcccctc
     6481 ggtccctacg tcaatctccc gaatccggcg tgggccacgg gccccttgag gagccctgcc
     6541 atctcaccct tcttcaaggc ggatgcaaga tccaaaaaaa cctcatccac cgcgtttcgg
     6601 tacttttcga tatgggtacc cttgtgggca aacgaggagt agaaggcttt tcctgcgagg
     6661 aaaccccgat cgagcatcat ctgggtaaag gcggtttgca tctcgagggc ctccgggcgg
     6721 gaaaaggtga aatgggagag gggcgggatc ccctccacca cgaggggaag acctgcatcc
     6781 gctgcggctc tggcccagag accctgcacc tgcttccccg tcctcacgag gtgttcgggg
     6841 acgttgcacc gcccgtactt gtcgatggtc gcgagggcgg caacaggccc gatccgttcg
     6901 gtccagtaag tgctgctgat gaaagtctcc tgggccgcgg acatggcatc gcctctccct
     6961 atgatggccg ccatgggata gccgttactc atccccttgg caaaaaccgc gatgtccggc
     7021 cggatcccgt agacgaggtg ggcacccccg gtattcaggc ggaatcccgc tgagacctca
     7081 tccacgacga gaagagctcc cgccgcggcc gcgagctcct ggacctcttc gagaaatccg
     7141 ggcacaggtt catggtccct gattggttcc atgaccacgg cggccaggga accatcatgc
     7201 cggtcgataa ttgcccggag atcctcgatc tcattgtacc ggaagggcac cgctgtaccc
     7261 ttcaaacccc ggggaacacc cctcggttca aggcccggca ggagatgtcc gtcgaggttc
     7321 cggtcatcgg caaggttcgc ggagaggtac cagtcgtgcc agccatggta cccgcagaag
     7381 gccacgatct ccctccctgt ccgggcgcgc gcgatacgca ccgccaccgc catggcctcg
     7441 cccccgcccc gtgcaaaccg gaccatctct gcccagggat ggagccggag gaggacctcg
     7501 gcgagctcga cttcctccgg gcagttcaga gtgcacatgg agccctggtc gatggcctcc
     7561 ttcaccgccc ggtccacgtc gggatcggca tacccgaggg ggcatgcccc cactcccagg
     7621 agggacatgt cgatgtaccg ccgcccgtcc aggtcggtga cctccacccc ctttgcggaa
     7681 cgatagtacg agggccacag atccgggagg aactgttcgc tgcgcttcga gaggagctgg
     7741 cttcctccag ggatgatcct ttttgcccgc tgccagaggg tcaaaccgga gttcttctct
     7801 ctcttcattc cgcccccagg atccgggtat ccttctcaag ggataacctg tatccctcat
     7861 ttcttttcag gtggctgttg ataccggaga gttccgggtg atcggcgagg agcctgacaa
     7921 cgtcctccag ggcaaaaagg tttttctgcg ggaacaggtg ccggtagacc tcccggataa
     7981 agagataatc ctcccgggtg tcaagcgtcc accggagatg ggacagatcc gtcaccgcac
     8041 ggaaattcac ggttttaaat ctgccgctcc tccgcatgaa gggtgtgaca tgctcacggt
     8101 ctgagggcag tcttgcctcg ctgtgggtga tttcgagaac ctgcctcgtg aagacctcga
     8161 catcgaggcc atcggggaag gtcgggggat cgacatttga gatatagtca gggttcttct
     8221 cctcggcctc gcggaccccc cgatcgatga ccgcgggatc catcagcggg caatccgccg
     8281 tgatcctcac aatcagatcc acggggacgc cttggcggtc caggtacctc accgctccat
     8341 ggtagcgggc caacacatcc tcggctggcc cccgatagac cggggtaccc cattccctgc
     8401 agagttcctt gatggcatca tcctccgccc tccgggtggt tgcgacgaga acccggtgta
     8461 cacgttgggc cgcctgtgta cgttgtacga catgccagag aaccggtttc ccgcagagat
     8521 ccatgaggac cttcccaggg aggcgcgtgg accccatccg cgcctggatg atggcaactg
     8581 cactcattcc cggtccccgt ggcaccggga aaccggtgca ggcagcaatg cagtatcatc
     8641 caggtccagc ccccgccggt agcccctgcc cctcaatcct ctggaatcct tcatcccccg
     8701 catgatcgta aagattttat tgggcctccg caacgatgtc ccggagatcc tcctttgtca
     8761 gccacacggt atttgagttg ctgtcgtacc ggaaatc
//
LOCUS       DATGDI010000115        17888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_98655, whole genome shotgun sequence.
ACCESSION   DATGDI010000115 DATGDI010000000
VERSION     DATGDI010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 17888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17888
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98655"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VKO45_04455"
     CDS             <1..647
                     /locus_tag="VKO45_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HMA05160.1"
                     /translation="DLSGMRIVATVVNAREAAHAAVLCPDLIEARIDLMAGDPSEELP
                     AIRSAFDGPVILTIRSRAEGGGFGGDPGAWWECLEPLLPFGDLVDVELPFSSFCPAIR
                     REGKGIIASRHVEGMPTREELRRLEADLRRYGDIPKIVVRPGTERDLLGLLEFTLGAE
                     RPICTGVLGEAFRFARVILPFFGSELAYTHAGTSAAPGQFALEDFKRIQALLGI"
     gene            complement(682..1584)
                     /locus_tag="VKO45_04460"
     CDS             complement(682..1584)
                     /locus_tag="VKO45_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05161.1"
                     /translation="MNAWIETYRRAYPVLLLLLVFFILVLPSQAIPYTAATAYSTDFS
                     TDPGWSTNNPATNYWDKDLREWHYLMRDGSGAYVNVKVPYHGESFTLAYDILPERTDY
                     QASVKFGLGDNDQVSVERLTIFTEFENSPYGRLIWIRSIDGQNQRREASSYYLSYGGP
                     TVQFTDGVWYHVVMDYRLETRSLTISVTRRNDSQPVWHYSLDSLSVFPTMNRIYMSKI
                     GDNANPNAIAEGYIDNVVFSLVYPEATLQPSGQAATPLTSGSPAGTTGGVPSSTARPV
                     PLSLLPAGCALAVLVLFAGRSRRS"
     gene            complement(1701..1904)
                     /locus_tag="VKO45_04465"
     CDS             complement(1701..1904)
                     /locus_tag="VKO45_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05162.1"
                     /translation="MAGGEDTYALETCAHCEGAGCCYCDKKGKVFVRKPSRKCHHCEG
                     DGCIYCGFTGWEGLRAKYPSCGE"
     gene            complement(1904..2929)
                     /gene="aroC"
                     /locus_tag="VKO45_04470"
     CDS             complement(1904..2929)
                     /gene="aroC"
                     /locus_tag="VKO45_04470"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HMA05163.1"
                     /translation="MNTLGRNFRITTFGESHGKALGVVIDGCPPGIPLTEGDIQPFLD
                     RRRPGKSPLESPRKEPDRVEILSGVFRGKTTGAPIALVVANTDVREEDYAELEEIFRP
                     GHADFTWQAKYGIRDHRGGGRSSGRETVARVAAGAVAAKFLAGTGVKITGRILEIHGK
                     RDPDAMTREIEKARDEGDSVGGIAEVLVAGCPAGLGDPVFSKLDALLAHALMGIGAVK
                     GVEIGEGFAAARMKGSKHNDAMTRKGFATNHAGGILGGISTGQEIVARIAVKPTPSIA
                     KTQRTVDRMGKEVSVSIRGRHDPCIVPRILPVAEAMVALVIMDAYLEQARYSALPGRE
                     AGSEGDA"
     gene            complement(2930..4309)
                     /locus_tag="VKO45_04475"
     CDS             complement(2930..4309)
                     /locus_tag="VKO45_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HMA05164.1"
                     /translation="MRIVLTGFRGTGKTTVGEILAGKLGIPSIDTDRAIEERAGRKIH
                     AIFREEGEPAFRELERQVIASLPEDRVVVSAGGGAVLDPENVARLRRGSLVFLLQAGA
                     GVISARIGGSDRPSLTGRPPAEEVKELLRARGPAYLRAADFCIASGDRSPDEVAGEIL
                     RILREGPVSPGSRTPWMSFIEKVPFPGRDRETVSRILRGDGGKRAGLCGIAGYPCLHS
                     KSPALWNRLFGRYGLPYFYTALEWPDIGEVIGAVRDLGIRALSVTIPFKERVVGLLDE
                     VDGEARAIGAVNTVVRCGDELHGYNTDWLGIRAPLGDLHGARAVVLGAGGAASAAVHA
                     LLSLDMEVTILARDPVKGGRLAARFGCRSAPLSSFREIRPDLVVNATPVGMLPDQSSL
                     LAAGDLEKGMTVFDLVYTPPETPLLAIARKAGCRVIPGTEMFIHQAREQFFHVTGIRV
                     PDTLVRELV"
     gene            complement(4306..5577)
                     /gene="aroA"
                     /locus_tag="VKO45_04480"
     CDS             complement(4306..5577)
                     /gene="aroA"
                     /locus_tag="VKO45_04480"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HMA05165.1"
                     /translation="MEVTLRKAGALDLRVTAPPSKSFTHRAMVAAGLARGSSTLVYPL
                     ISKDTLTTGDALRALGTLVAWEKKYAHITGVGGRFPTTAETVLDLGDSGTSMRFFTAL
                     ALLAKNPVVLRGSGRMHERPIGPLADALGKLGGEVRYLGKEGYPPLRVSGSFRGGEVV
                     VDGSMSSQFVSAILLAAPCGEGDLTLTVSPPPVSRSYLDITTEVMKAFHAPPEREGYL
                     RFHVKGGTGYWFHGYQIEGDFSSASYYLAMGAVCGGRVMVDNLNRSSVQGDRRFIDIL
                     EQMGCRIGWDQWDTVTLESDGNLSGVDVDMSASPDTVQTLCAVAAFAKGPTRIRGIGH
                     LRHKESDRIEATAVAFRNLGGDVRVEGDTMVIEPGPLHGGVVDPAGDHRTAMAFAVVG
                     LGAGGITIRGAECVAKSFPEFWEPLRRARLL"
     gene            complement(5568..6365)
                     /locus_tag="VKO45_04485"
     CDS             complement(5568..6365)
                     /locus_tag="VKO45_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HMA05166.1"
                     /translation="MIAALGPEGTFSHELALSLFGEGEVQLLPTIRRIFDEVEKGGCD
                     GVVPLENSEVGGVGPTLDCLQTHQVFITGEAYMEVHHHLAGFGKLEDLTMIYVHPQTH
                     EQCSEFLEKLGLEVIHTSSNAASAQAMKERKHAGAIVSELAARIYRIPIIRRDVQNTT
                     GNTTRFILISSRPPAEARPAKCSILIDPREDRPGLLHDLLSVFARRGINLTRIESRPS
                     KRGMGSYIFFLDFATSRGWEVAIRELHGLTHVKNLGCYRRVEVPGWR"
     gene            complement(6362..7189)
                     /locus_tag="VKO45_04490"
     CDS             complement(6362..7189)
                     /locus_tag="VKO45_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HMA05167.1"
                     /translation="MIAGIIGGTGRMGRLFAPILERHGLEVRVSGRTTALSNRRLAEE
                     SDLVMVSVPIRSTVEVIQEIAPLLTEEQLLCDLTSLKVEPVKAMLSSRAQVLGMHPLF
                     GPTVSSLRSQTVILTPARCGTPLASRIPEILRAEGANLLVMDPEDHDRLMAVIQGLTH
                     FGNLCMAEAIRRTGTDLAAALGATSPVYRIQMDLVGRLLSQDPALYGDMLQLNPHVPS
                     VLASFEAAAADLRARVEGGELPAFVEFFGRVAELYRPYRDQAAVETDALIDFMAGRT"
     gene            complement(7194..8183)
                     /locus_tag="VKO45_04495"
     CDS             complement(7194..8183)
                     /locus_tag="VKO45_04495"
                     /EC_number="1.4.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HMA05168.1"
                     /translation="MKELWVDVRPWKKDIATTAIESGADVLVVESAAKVRELGRVTTV
                     ATDGDLVPGRDVFEIEVKDKPSEEEAVRRSREGYVLVRTPDWTVIPLENLVSRADRII
                     AAVKDRKEAELALGALEKGVRGVLLKTGNPSVVRDVARLIKETAGKAELVPFTITAIR
                     PVGMGDRVCVDTCTMMGEGQGILVGNTSSAFLLVQAETQENPYVAPRPFRVNAGAVHA
                     YALMPGGKTAYLSEIDISSRVALVSADGRVTEACVGRTKIEKRPLLLVEAEGGGGVRA
                     SLILQNAETVRLVGEDGRSISVAALKVGDRVLGATAEGGRHFGVAVKESILEK"
     gene            complement(8180..8962)
                     /locus_tag="VKO45_04500"
     CDS             complement(8180..8962)
                     /locus_tag="VKO45_04500"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HMA05169.1"
                     /translation="MIGKEIRIERIINRNTGRSVIIPMDHGFSLGQIEGLTNMTNVIT
                     DVAKGGANAIILHKGMVKAGHRKHGKDIGLIVHLSGSTSMNPDPNDKVIVCTVEEAIA
                     LGADAVSIHVNLGAPNESRMIESAGEISRACTRWGIPLLIMIYPRGKNVDSTSPAAIG
                     HCVRVAEELGADFIKTNYPGDPESFRKIVSACSVPVMIAGGEKAGDAETLKIISDSVK
                     AGGAGVCMGRNAFQRTNTTRFVQAISKVVHKNLDPGEAMGML"
     gene            complement(8983..9774)
                     /locus_tag="VKO45_04505"
     CDS             complement(8983..9774)
                     /locus_tag="VKO45_04505"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HMA05170.1"
                     /translation="MRGKEIRLERIMNRNTGRTIIVPMDHGVTNGPMKGLIDMGRTVE
                     LVADGGANAVLGHLGLALHGHRRHGRDVGLILHLSGSTSLSPDPNEKVLVNTVQNAMK
                     MGADGVSIHVNVGAESEARMLRDLGKIAVECMDWGMPLLAMMYPRGAKIKDEHGVEPV
                     ALAARVAAELGADLVKTVYTGDPDSFREVTRGCPVPVVVAGGSKTDERATLELIAGAM
                     EGGAAGISIGRNAFQHPAPDRFVRAAAAIVHESRSVEEALKIIKG"
     gene            complement(10254..11681)
                     /locus_tag="VKO45_04510"
     CDS             complement(10254..11681)
                     /locus_tag="VKO45_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA05171.1"
                     /translation="MIFEKVVSVGLAHNSYLVGSGGEAAVIDPRRDIDVYLDIAREED
                     LRITHVFETHRNEDYVIGSLELARATGCEIHHGGRLDFGYGSPVREGDSFRVGTLELR
                     ILETPGHTEESISLVLLDTEVSGTPLMVFTGDALFAGEVGRTDFYPDRMEQVAGELHD
                     SLHRKILPLGDGVIICPAHGGGSVCGADISDLPWTTIGYERETSPILRLERGAFIRKK
                     VAERHHYPAYFSMMEKLNHEGSPPLAWPLFLLNLTVPSVAELRKKGAQVVDIRSPASF
                     AGGHLPGSLHVWREGLSAFIGYYLNYDDPIVVVDDFNEDLVEVRREFARLGYDNLAGC
                     LEGGFMAWAKAAEPVGRVTSWTVHDLKAHLGDPSLFLLDLRDEPNYRKHGHLPGAHQV
                     YAGEFPSFICKVPEDMDIVCFCDAGYKSSIAASLLLRAGFTRVTVVLGSMAAWEKAGY
                     PVEKESGERKDAERGGTQGLEIGPS"
     gene            11819..13132
                     /locus_tag="VKO45_04515"
     CDS             11819..13132
                     /locus_tag="VKO45_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05172.1"
                     /translation="MGSETGKQAGGNLLPAPHEIHLRRKLGILILLLSFSCLVVGFLF
                     VPVALATGWYLRRNRGYHLFADLFLVLAGIIFYTTNIPLFAPPPWFMRVFLLNFSLSG
                     LPFAVLLSLAFIEKILILSSALYLLAGDLLVRLGPSFPGPERASFPRVILPIFALLAV
                     IPLFLPLGLATGPEGSWSSGGCPPSLDAFSMEGDSITLIADPEAGRWTYRITARNRLD
                     REASILRITGQKPFPGLLGSLNLLSRTVILAPPYHPGIEVNGGVRTEESILVRPGETA
                     VIRILSDEPIFSLTILDSGGCEFSRSFLAPAGELPDYTIDATPAYDPGVRILLTEEFP
                     PGSDPGKTVFHPGENIYLSETGLPAGTAYGFQVKDSGGRIFVWPPLRMQQMARVKTGT
                     GPETSGTFNTRQDPLEPGEFTVELVRFGEKKGWIIASVNLSVIPA"
     gene            complement(13157..14041)
                     /locus_tag="VKO45_04520"
     CDS             complement(13157..14041)
                     /locus_tag="VKO45_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HMA05173.1"
                     /translation="MIEGQNLPDKARRAVLASQRGETTEHGIYRALALKASEAGNRKV
                     LEEIAAVEQGHYGFFRSFTGTDIAPDRIRIAFYLLIYHIFGITFSLKLMERGEKRAQK
                     LYRDLLPVVPGLDAVLLDEEGHEKALIGLIDERRLQYTGAIVLGLNDALVELTGTLAG
                     FTLALQDTRVIAMAGLITGIAAALSMGSAEYLSTKSEGGERNPLRAAMYTGIAYIITV
                     LFLVAPYLILASYYLALAVTILNAIVVILVFTFYISVTRDVDFRGRFVEMAGLSLGVA
                     ALSFLMGLLVRVLLGVNA"
     gene            complement(14038..14829)
                     /locus_tag="VKO45_04525"
     CDS             complement(14038..14829)
                     /locus_tag="VKO45_04525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HMA05174.1"
                     /translation="MKPYFAADGFELYHADSLGFLEGLPEDSVDLIFADPPYRLSNDG
                     FTCHAGRRAPVNKGSWDESRGTEEDFRGHLAWIRACQRVLAPAGSIWISGTYHSIYAC
                     GYALQIAGYHLLNDICWFKPNAPPNLSGRYFTASHETLLWAAKGPECRHTFNYDLVKN
                     GSWPGDALKRPHRQMRSVWSIPAPRPGEKRFGRHPTQKPLELLLRIILASSKKGDLVL
                     DPFTGSSTTGLACTRTGRRFIGVDREEKFLELSVKRYLASREEGT"
     gene            15108..16118
                     /locus_tag="VKO45_04530"
     CDS             15108..16118
                     /locus_tag="VKO45_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMA05175.1"
                     /translation="MRPDGENGGRAAPDPEIAVLYVDDEPALLELTTLFLGKAHGDLA
                     IDTASSARVALEKLRLRPYDVIVSDYLMQGMDGIEFLKGLRAGGCSIPFIIFTGKGRE
                     DVAIEALNSGADFYLQKGGDPRSQFAELANMIRQAARKRRAEEALRAHEERLHAIIQG
                     SPIPQFVIGRDHRVIYWNRALEQNTGVPASEVLGTRGQWKAFYPFERPCLCDLLVDGT
                     PGEISLWYGDKAGPSRHVEGACEVKDFFPNMGDHGRWLLFTSVAIRDRTGAILGALET
                     LEDITDQVSAEENLRERECELEETRARLDGIFQAHPGTPDRGLHGGSLGTGNLPVPPA
                     EQ"
     gene            16409..17098
                     /locus_tag="VKO45_04535"
     CDS             16409..17098
                     /locus_tag="VKO45_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05176.1"
                     /translation="MASSPDPSRFGEYMKRVSHVIALQGIFGAILLLTRYLPDQSVPL
                     LARGQEALTLLDVNSLTVFALFLVLSIAILRQESDFALPREDPFVRVLEGYGSPAGST
                     TLFRVVHGLFTVVMILVYLTAPPITAVPLAPTLHQEAGVLVLFVIVVFSSRAVSLAAM
                     RFFDRTMAGLRHAFACLIYLLGLGLIFGGAITGIENLLIAGMAVISLEILYVVIREDR
                     RVRAGESSRDG"
     gene            17205..17804
                     /gene="hxlB"
                     /locus_tag="VKO45_04540"
     CDS             17205..17804
                     /gene="hxlB"
                     /locus_tag="VKO45_04540"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HMA05177.1"
                     /translation="MEEHPVQDMMRLMASKVRSIANGISGEEVEGMIREILAAKRVYV
                     MGAGRSGLVARAFAMRLMHLGLTSYVVGETVTPAVNPEDLVIVFSGSGETRTVADIAE
                     SAKAIGARLCLITSKRDSRIGRIADCCVVIESQRDTEGDEHAEFEIRQMMGKHKSFAP
                     LGTLFETASVMFADAVISRLMEIKEINVETLKGRHVNIE"
BASE COUNT         3021 a         5922 c         5749 g         3196 t
ORIGIN      
        1 ccgatctctc cggcatgagg atcgtggcca cggtcgtgaa cgcgcgggag gccgcccatg
       61 cggcggtcct ctgccccgat ctcatcgagg cccggatcga cctcatggcc ggagatccct
      121 ctgaggagct gccggccatc cgatcggcct tcgacgggcc cgtcatcctc accatccgga
      181 gcagggccga gggaggggga ttcggcggag atccgggggc atggtgggaa tgcctcgagc
      241 ccctcctccc cttcggggac ctcgtggacg tcgagttgcc cttctcctcc ttctgccctg
      301 cgatacgccg ggaggggaag gggatcatcg catcccgcca tgtggagggg atgcccaccc
      361 gcgaggaact ccggcggttg gaagcggacc tcaggaggta cggggatatc cccaagatcg
      421 tcgtccgtcc ggggaccgaa cgggatctcc tggggctcct ggagttcacc ctcggggccg
      481 aaaggcccat ctgtaccggt gttctcggcg aggcgttccg gttcgcgagg gtaatccttc
      541 ccttcttcgg ctcggagctc gcgtacaccc acgcggggac ctccgccgcc ccgggccagt
      601 ttgccctcga ggatttcaaa aggatccagg ccctcctcgg gatctgacgg ggcggaaggg
      661 cccgaaaaac ggggaaggag gtcagctccg cctggaccgt ccggcgaaga gaaccaggac
      721 cgcgagggcg cacccggcgg gaaggaggga gaggggaacg ggtctggcag tcgatgatgg
      781 aacccctccc gtcgtacccg cgggagatcc cgaggtgagg ggtgtcgcgg cctgaccgga
      841 gggctgaagg gttgcctccg ggtagaccag ggagaagacc acgttgtcga tgtacccctc
      901 ggcgatggcg ttcgggttcg cattgtcacc gatcttggac atgtagatcc ggttcatggt
      961 ggggaagacc gagagggagt ccagggagta atgccagact ggctgcgagt cattgcgccg
     1021 ggtcaccgag atcgtgaggc ttctcgtttc caaccggtag tccatgacca cgtggtacca
     1081 taccccgtcg gtgaactgga ccgtgggccc gccgtaggag aggtagtagg aggatgcctc
     1141 cctccgctgg ttctggccgt cgatggaccg gatccagata agcctcccgt agggggagtt
     1201 ttcgaactcc gtgaagatgg tgagccgctc gacggacacc tggtcgttat ccccgaggcc
     1261 gaacttcacg ctcgcctggt aatcggtccg ctccggtaaa atatcgtagg caagggtgaa
     1321 ggactcgccg tggtaaggaa ccttcacgtt cacgtaggcc ccgctcccgt ccctcatcag
     1381 gtagtgccat tcccgcaggt ccttgtccca gtagttggtc gccgggttgt tcgtggacca
     1441 gccggggtct gtggagaaat ccgtgctgta cgcggtggcc gccgtatacg ggatggcctg
     1501 ggacgggagg accaggatga aaaacacgag gaggaggaga agaaccgggt atgcccgcct
     1561 gtacgtttcg atccatgcat tcacgctgat ccctccttct tctagacctg agatgggacc
     1621 ccggggttta aaaatgaacg ggacaggggg agccgccgga ccatcatcac cgtcgggcag
     1681 agtgaaccgc ccgcgaggta ttattcgccg caggaagggt acttcgccct gagaccctcc
     1741 cagccggtga acccgcagta gatgcagccg tccccttcgc agtgatggca cttcctcgac
     1801 ggcttgcgga caaaaacctt tcccttcttg tcgcagtagc agcacccagc accctcgcag
     1861 tgggcgcagg tctcgagggc ataggtatct tccccgccag ccatcaggca tccccctcag
     1921 agccggcctc ccggccgggc agggcagagt acctggcctg ctcgaggtac gcgtccatga
     1981 tgaccagggc aaccatggcc tctgcgaccg ggaggatccg gggcacgatg caggggtcgt
     2041 gcctgccccg gatggagacg gacacctctt tccccatcct gtcgacggtc cgctgggtct
     2101 ttgcgatgga gggggtcggc ttgaccgcga tccgggccac gatctcctgg ccggtgctga
     2161 ttcccccgag aattcctccg gcatggttcg tcgcgaaccc cttccgggtc atggcgtcgt
     2221 tgtgcttgct tcccttcatc cgggccgcgg caaagccctc gccgatctcc actcccttca
     2281 ccgctccgat ccccatgagg gcgtgggcga ggagggcgtc gagcttggaa aagaccgggt
     2341 ccccgaggcc ggccgggcaa cccgcgacca ggacctcggc gatcccgcct accgagtcac
     2401 cctcgtcccg ggccttctcg atctccctcg tcatcgcgtc cgggtccctc ttcccgtgaa
     2461 tctcgaggat cctccccgtg atcttgaccc cggtcccggc gagaaacttc gcggccaccg
     2521 ccccggccgc gacccgggcc acggtctccc tccccgagga ccggcctcct ccccggtgat
     2581 ccctgatccc gtacttggcc tgccaggtga agtctgcgtg gccaggccgg aagatctcct
     2641 cgagctcggc gtagtcctcc tcccgcacgt cggtgttcgc caccacgagg gcgatggggg
     2701 ccccggtggt cttcccccgg aagacgcccg agaggatctc cacccggtcg ggttccttcc
     2761 ggggtgactc cagcgggctc ttcccgggcc gcctccggtc caggaagggc tggatgtccc
     2821 cctcggtgag ggggatcccc ggcgggcagc cgtcgatgac gacccccagg gctttcccat
     2881 ggctctcgcc gaaggtggtg atacggaagt tccggccgag ggtattcact cacaccagct
     2941 ccctgacgag cgtatcgggg acccggatac ccgtgacatg gaagaactgt tcgcgggcct
     3001 ggtggatgaa catctcggtc cccgggatga cccggcaccc cgccttacgc gcaatggcga
     3061 ggagcggggt ctccgggggg gtgtacacga ggtcgaagac ggtcatcccc ttctccaggt
     3121 cccctgcggc gaggagggag gattggtccg ggagcatccc gacgggggtc gcgttcacca
     3181 cgagatccgg ccggatctcc ctgaaggagg agaggggggc cgagcggcac ccgaaccggg
     3241 cggcgaggcg ccctcccttc accgggtcgc gggcgaggat ggtgacctcc atgtccaggg
     3301 agagaagggc gtggaccgcc gcggaggcgg cccctcccgc cccgagcacc accgcccggg
     3361 cgccgtggag atccccgagg ggggcccgga tcccgagcca gtcggtgttg tatccatgga
     3421 gctcgtcccc gcaccggacc accgtgttca ccgcgccgat ggcccgggcc tcaccgtcga
     3481 cctcgtccag gagacccacg accctctcct tgaaggggat ggtgaccgag agggcccgga
     3541 tgcccaggtc ccggaccgcc ccgatcacct ccccgatatc cggccactcg agggcggtgt
     3601 agaagtaggg gagcccgtac ctcccgaaga gccggttcca gagggccggg cttttactgt
     3661 ggagacacgg gtaccccgcg atcccgcaga ggccggcccg cttcccgcca tcgccgcgga
     3721 ggatgcggga gaccgtttcc cggtccctgc cggggaacgg gaccttttcg ataaagctca
     3781 tccacggggt ccgggatccc ggggaaacgg gtccttcgcg gaggatcctc aagatctcgc
     3841 ccgcgacctc atcgggggac cggtccccgc tcgcgatgca gaagtcagcg gcgcggaggt
     3901 atgccggccc ccgtgcccgg aggagttcct tcacctcctc ggcagggggg cggccggtga
     3961 gggagggacg gtcgctcccc ccgatccggg cggagatgac ccctgcccct gcctggagga
     4021 ggaagacgag ggacccccgc ctcagccggg ccacgttctc cgggtcgagg accgccccgc
     4081 ccccggcgct gaccacaacc cggtcttcgg ggagggaggc gatgacctgc cgctccagct
     4141 cccggaaggc gggttccccc tcctcgcgga agatggcgtg aatcttcctc ccggcccgtt
     4201 cctcgatggc ccggtcggtg tcgatggagg ggattcccag tttcccggca aggatttcac
     4261 cgaccgtggt cttccccgtc ccccggaagc cggtgagaac gatcctcaca ggagcctcgc
     4321 cctcctgaga ggttcccaga actccgggaa ggacttcgcc acgcactccg cacccctgat
     4381 ggtgatgccc ccggcgccga ggccgaccac cgcgaaggcc atggcggtcc ggtggtctcc
     4441 ggcgggatcc acgacgcccc cgtggagggg cccgggctct ataaccatcg tgtccccctc
     4501 cacccggaca tccccgccga ggttcctgaa ggccacggcc gtggcctcga tccggtcgct
     4561 ctccttgtgc ctgagatggc cgatcccccg gatccgggtc gggcctttcg cgaaggcggc
     4621 caccgcgcag agggtctgga ccgtgtccgg gctcgccgac atgtccacgt ccactccgga
     4681 gagattcccg tcgctctcca gggtgaccgt gtcccactgg tcccacccga tccggcatcc
     4741 catctgttcc aggatatcga tgaaccgacg atcaccctgg acagacgacc ggttcaggtt
     4801 gtcgaccatc accctccccc cgcagaccgc gcccatggca aggtagtagg aggccgagga
     4861 gaagtccccc tcgatctggt agccatggaa ccagtacccc gttcctccct tcacgtggaa
     4921 ccgcaggtat ccctcccgtt ccgggggggc atggaaggcc ttcatgacct ctgtcgtgat
     4981 atccaggtag gaccgcgaga ccggtggggg gctcacggtg agggtgaggt ctccctcgcc
     5041 gcagggggcg gcaaggagga tggccgagac gaactggctg ctcatgctcc cgtccaccac
     5101 cacctccccg ccccggaagc tgcccgaaac tctgagaggg ggatacccct ccttcccgag
     5161 gtaccggacc tctcctccca gcttcccgag ggcatcggcg aggggaccga tgggccgctc
     5221 gtgcatcctc ccgctccccc gaagcacgac gggattcttc gcgaggaggg cgagggcggt
     5281 gaagaaccgc atgctcgtcc ccgagtcccc gagatcgagg accgtttccg ccgtggtggg
     5341 gaatctcccg ccgacacccg tgatgtgcgc gtacttcttc tcccaggcga ccagggtccc
     5401 gagcgcccgg agggcatccc ccgtggtcag cgtgtccttc gagatgaggg ggtacacgag
     5461 cgtggaggag ccgcgggcga ggcctgctgc caccatggcc cggtgggtga aggacttgga
     5521 ggggggcgcg gtgacccgga ggtcgagggc ccccgccttc cggagggtca cctccatccc
     5581 ggcacctcca ccctccggta gcaccccagg ttcttcacat gggtgagccc gtggagctcc
     5641 cggatggcca cctcccagcc cctgctcgtc gcaaagtcaa ggaagaagat gtagctcccc
     5701 attcctctct tcgaaggccg ggactcgatc ctggtgaggt tgatccccct ccgcgcgaaa
     5761 acggagagga ggtcatggag gaggccaggc cggtcctccc ggggatcgat gaggatgctg
     5821 cacttggccg gcctcgcctc ggcgggcggg cgggaggaga tgaggatgaa ccgggtggtg
     5881 ttcccggtgg tgttctggac atcccggcgg atgatgggga tacggtagat ccgggcggcc
     5941 agctccgaga cgatggcccc cgcgtgcttc ctctccttca tcgcctgggc gctcgccgcg
     6001 ttggaggagg tgtggatgac ctcgagcccg agcttctcca ggaactcgct gcactgctca
     6061 tgggtctggg gatggacgta gatcatggtg aggtcctcga gtttcccgaa gccggcgagg
     6121 tggtggtgta cctccatgta cgcctcgccc gtgatgaaaa cctggtgggt ctggaggcag
     6181 tcgagggtgg ggcccacccc cccgacctcg ctgttctcga gagggactac tccgtcgcat
     6241 ccccccttct ccacctcgtc gaagatcctc cggatggtgg gcaggagctg cacctccccc
     6301 tccccgaaga gggagagggc cagctcgtgg ctgaaggtcc cctctggacc tagggcggcg
     6361 atcatgtcct ccccgccatg aagtcgatga gggcatcggt ctctaccgcc gcctggtccc
     6421 ggtaggggcg gtacagctcc gcgacccgcc cgaagaactc gacgaaggcc gggagctccc
     6481 cgccctccac ccgggcccgg agatcggccg ccgccgcctc gaaggaggcg agcaccgacg
     6541 gcacgtgggg gttcagctgg agcatgtccc cgtagagggc ggggtcctgg gagaggagcc
     6601 tcccgacaag atccatctgg atccggtaga ccggcgaggt cgccccgagg gcggcggcga
     6661 gatcggtccc tgtcctccgg atggcctcgg ccatgcagag gttcccgaag tgggtgagcc
     6721 cctggatcac cgccatgagc cggtcatggt cttcggggtc catgacgagg aggttcgcac
     6781 cctccgccct gaggatctcg gggatccggg aggcgagagg ggtgccgcac cgggcgggcg
     6841 tgaggatgac cgtctggctc ctgagggagg agaccgtcgg cccgaagagg ggatgcatcc
     6901 cgagaacctg ggccctcgac gagagcatcg ccttcacggg ttccaccttc agggaggtga
     6961 ggtcgcagag cagctgctcc tcagtgagaa ggggagcgat ctcctggatg acttccaccg
     7021 tcgatctgat gggcaccgag accatcacga ggtcgctctc ctccgcgagg cggcggttcg
     7081 aaagggcggt ggtcctcccc gaaaccctga cctcgaggcc gtgcctctcg aggatgggtg
     7141 caaagagccg gcccatcctt cccgttcccc cgatgatacc cgcgatcatg gcctcacttc
     7201 tcgaggatgc tctccttcac cgcgaccccg aagtggcggc ctccctcggc tgtcgccccg
     7261 aggacccggt cccccacctt cagggcggcg acggagatgg acctcccgtc ctcgcccacg
     7321 agccgcaccg tctccgcgtt ctggaggatg aggctcgcgc gcaccccgcc gcccccctcg
     7381 gcctccacga ggaggagggg ccttttttcg atcttcgtcc tccccacgca ggcctcggtg
     7441 accctcccgt ccgccgagac gagggccacc cgcgaggaga tatcgatctc cgagaggtag
     7501 gcggtcttcc caccgggcat gagggcatag gcatgcaccg cccctgcgtt cacccggaag
     7561 ggccttgggg caacgtaggg gttctcctgg gtctcggcct ggacgaggag gaaggccgag
     7621 gaggtgttcc ccacgaggat gccctgtccc tcacccatca tcgtgcaggt gtcgacgcag
     7681 acccggtcgc ccatgccgac ggggcggatg gccgtgatgg tgaagggcac gagctcggcc
     7741 tttcccgcgg tctccttgat gagcctcgcc acgtcgcgga cgaccgaggg gttcccggtc
     7801 ttgaggagga ccccccggac ccccttctcc agggccccga gggcgagctc cgcctccttc
     7861 cggtccttca ccgcggcgat gatccggtcg gccctcgaca ccaggttctc gagggggatg
     7921 accgtccagt ccggggtcct cacgaggaca tacccctccc ggctcctcct gaccgcctcc
     7981 tcctccgagg gtttgtcctt cacctcgatc tcgaatacat ccctcccggg cacgagatcc
     8041 ccgtcggtgg cgacggtcgt caccctcccg agctccctga cctttgccgc cgactcgacc
     8101 acgaggacat cggcgccgct ctcgatggcg gtggtcgcga tgtccttctt ccacggacgg
     8161 acgtcgaccc agagttcctt catagcatcc ccatggcctc gccggggtcg aggttcttgt
     8221 gcacgacctt cgagatggcc tggacgaagc gggtcgtgtt ggtccgctgg aaggcattcc
     8281 ttcccatgca gaccccggcg cctccggcct tcaccgagtc actgatgatc ttcagggtct
     8341 ccgcgtcccc ggccttttcc ccgcccgcga tcatgaccgg gaccgagcat gccgagacga
     8401 tctttctgaa ggactcgggg tcgcccgggt agttggtctt gatgaagtct gccccgagct
     8461 cctccgcgac cctcacgcag tggccgatgg ccgcgggcga ggtgctgtcg acgttcttcc
     8521 ccctcgggta gatcatgatg agcaggggga ttccccaccg ggtgcaggcc ctcgagatct
     8581 cgcccgcgga ctcgatcatc cgggactcgt tcggcgcgcc gaggttcacg tggatggaga
     8641 cggcatcggc cccgagggcg atggcctcct ccaccgtgca gacgatgacc ttgtcgttcg
     8701 ggtccgggtt catggaggtc gagccggaga gatggacgat gaggccgatg tccttcccgt
     8761 gcttccggtg gccggccttc accatcccct tgtgcaggat gatggcgttc gccccgccct
     8821 tcgcgacgtc ggtgatgacg ttcgtcatgt tcgtgagtcc ctcgatctgc cccagggaga
     8881 acccgtggtc catcggaatg atgacgctcc tccccgtgtt ccggttgatg atccgctcaa
     8941 tacgaatttc ctttccgatc atgtttcctt cctccttcct cgttatccct tgatgatctt
     9001 cagtgcctct tcaacgcttc gactctcatg cacgatggcc gcggccgccc ggacgaaccg
     9061 gtcgggggcc gggtgctgga aggcgttccg gccgatggag ataccggccg cgccgccctc
     9121 catcgccccc gcgatgagct cgagagtggc ccgctcgtcg gtcttcgagc cgccggccac
     9181 caccaccggg accgggcagc cccgggtcac ctcccggaag ctgtcgggat cccccgtgta
     9241 cacggtcttc acgagatcgg ccccgagctc cgccgccacc cgggcggcga gggcgaccgg
     9301 ctccaccccg tgctcatcct tgatcttcgc ccccctcggg tacatcatgg cgaggagggg
     9361 catcccccaa tccatgcact cgacggcgat ctttcccagg tcccggagca tccgggcctc
     9421 ggactcggcc ccgacgttca cgtggatgga aaccccgtcg gcccccatct tcatcgcgtt
     9481 ctgcacggtg ttcacgagca ccttctcgtt cggatcggga gaaagggacg tcgagcccga
     9541 gaggtggagg atgagcccca cgtcccgccc gtgcctgcgg tggccgtgga gggcgaggcc
     9601 caggtggcca agcaccgcgt tcgcgccccc gtcggccacg agctccacgg tccttcccat
     9661 gtcgatgagc cccttcatgg ggccgttcgt caccccgtgg tccatgggga cgatgatggt
     9721 cctccccgtg ttccggttca tgattcgttc cagacggatt tcttttcctc tcatgtacaa
     9781 cctcccccgc cggaaggcgg cccatcggag gttgagaggt ccggaaccgt tccagcggcg
     9841 ggaaaactac ccgaaatacc cgtaaaagcg ataatagcta aagacagcag ccgctttccg
     9901 gacggaattc agagaactgt tcgctttctg caggacggag gcttcactcg gggagataaa
     9961 ggcaatcccg ggcgaaaagg catccaccac gcattggcta agatatagca tagccacagt
    10021 atttaaaatt accgagaatt attccatggg gtctcgttgc ccgggacagg cctatcctta
    10081 cgcgggacgc cgaagcggcg ggggtgagga cgcacgcctg aacctgcgac ttcgtcgctc
    10141 caggtcgggt tctgatgaac cctccctccc ccgaggagcg tccccctgga gccagcaggg
    10201 aatcatcgcc tcacacctgc cccgggctcc gattccgtat cgtgggggtt cagttacgac
    10261 ggcccgatct ccagtccctg cgtccccccg cgttcggcat cctttctctc tccggactcc
    10321 ttctccaccg ggtaccccgc cttttcccac gcggccatgg agccgaggac gaccgtgacc
    10381 cgggtgaaac ctgcccggag gagcagggaa gccgcgatgg aggacttgta gcctgcgtcg
    10441 cagaaacaga cgatatccat gtcctcgggg accttgcaga tgaaggaggg aaactcgccg
    10501 gcgtacacct ggtgggcgcc gggcaggtgg ccgtgtttcc ggtagttcgg ctcgtccctg
    10561 agatcgagga ggaacaggga gggatcgccg aggtgtgcct tcaggtcatg gacggtccag
    10621 gatgtcaccc tccccaccgg ttccgcggcc ttcgcccatg ccatgaagcc cccctcgagg
    10681 caacctgcca ggttgtcgta cccgagtcgg gcgaactccc ggcggacctc cacgaggtcc
    10741 tcgttaaaat catccacgac gacgatgggg tcgtcgtaat tcaggtagta cccgatgaag
    10801 gccgacagtc cctcccgcca gacatggagg ctcccgggca ggtgtccgcc ggcgaaggat
    10861 gcgggggacc ggatgtccac gacctgggct cccttcttcc ggagttccgc caccgagggg
    10921 acggtcaggt taaggaggaa tagcggccac gcaaggggag gggagccctc gtggttgagt
    10981 ttctccatca tggagaagta ggccgggtaa tggtgccgct cggccacctt cttcctgatg
    11041 aaggcccccc tctccagccg gaggatgggg ctcgtctccc tctcatagcc gatggtcgtc
    11101 caggggaggt cgctgatatc ggccccgcag actgacccgc caccgtgggc ggggcagatg
    11161 atgaccccgt ccccgagggg gagtatcttc cggtggaggc tgtcgtggag ctctccggcc
    11221 acctgctcca tccggtccgg gtagaagtcc gtgcgcccga cctcccccgc aaacagggca
    11281 tcgccggtga agaccatgag gggggtaccc gagacctcgg tgtccaggag gacgagggat
    11341 atgctctcct ccgtgtggcc cggagtctcc aggatccgga gctccagggt cccgacgcgg
    11401 aagctgtctc cctcgcggac gggtgacccg tacccgaagt ccagtcttcc cccgtgatgg
    11461 atctcgcagc ccgtggcccg cgcgagctcg agagaaccga tcacgtagtc ctcgttccgg
    11521 tgcgtctcga agacgtgggt gatgcggaga tcctcctctc tcgcgatatc caggtagaca
    11581 tcgatgtccc ggcgcggatc gatgaccgcc gcctcgcccc cggagcccac caggtaggag
    11641 ttgtgggcga gcccgacgga gaccactttt tcgaagatca tgaatgcccc ccggtttccc
    11701 ttttccggga ggggagatat acccctgcct catttatagg ttatgccctg ccgggaggtt
    11761 cctccagcca gccgggaggg aggtagaatc aaatatccat gagaggacag attcatgtgt
    11821 gggatcggag acggggaaac aggccggcgg gaatctcctt ccggctcccc atgagataca
    11881 cctcaggagg aagcttggca tcctcatcct cctcctctcc ttttcctgcc tcgtggtcgg
    11941 tttcctcttc gtgccggtcg ccctcgccac agggtggtat ctgcgaagga accggggata
    12001 ccacctcttc gccgacctct tcctcgttct tgccggtatc atcttctaca cgacgaacat
    12061 ccccctcttc gcccctccac cctggttcat gcgggtattc ctgttgaatt tcagcctgtc
    12121 cggcctgccc ttcgcggtgc tcctatccct cgccttcatc gaaaagatcc tgatcctctc
    12181 ctctgccctc taccttctcg cgggcgacct cctggtccgc ctcgggccgt ccttccccgg
    12241 cccggaaagg gcgagcttcc cccgcgtgat actccccatc tttgccctcc tcgccgttat
    12301 ccccctcttc ctccctctgg gcctggccac gggacccgag gggagctgga gctccggtgg
    12361 atgtccccct tccctggacg ccttcagcat ggaaggcgat tccataacac tcatcgctga
    12421 ccccgaagcc ggccgatgga cgtaccggat cacagcccgg aaccggctgg accgggaggc
    12481 gtcgatcctg agaatcaccg gccagaaacc cttcccggga ttactgggga gtctcaacct
    12541 cttatcgaga accgtgatcc ttgcaccccc gtaccacccg ggtatcgagg tgaacggcgg
    12601 agtgagaaca gaggagagca tcctggtgag gccgggagaa acggccgtta tcaggattct
    12661 ctccgatgaa ccgatctttt ccctgaccat cctggacagc ggcgggtgcg agttcagccg
    12721 ttccttcctc gcccccgcgg gagagctgcc ggattatacc atcgatgcga cccccgccta
    12781 tgatccgggc gtgcggattc tcctcacgga ggaattcccc cccggttccg atccggggaa
    12841 aacggtgttc catccgggtg agaacatcta cttgagcgaa acagggctcc ccgcagggac
    12901 ggcttacggg ttccaggtaa aagattccgg cggaaggatc ttcgtgtggc cgcctctgcg
    12961 gatgcagcag atggcaaggg tgaaaacggg aaccggaccc gagacttccg gaaccttcaa
    13021 cacgaggcag gatccccttg agcctggcga attcacggtg gaactggtca ggttcgggga
    13081 aaagaaggga tggatcatcg catcagtaaa cctgtcagtc atccctgcgt gacctcctcc
    13141 ccccctcacc gcccgcctac gcgttcaccc cgaggaggac ccggacaagg agccccatga
    13201 ggaaggagag ggccgcgacc ccgagggaga ggccggccat ctcgacgaac cttccccgga
    13261 agtccacgtc ccgggtcacc gagatgtaaa aggtgaagac gaggatgacg acgatggcat
    13321 tgaggatggt caccgcgagg gcgaggtaat agctcgcgag gatgaggtac ggcgcgacaa
    13381 ggaagaggac ggtgatgatg tacgcgatgc cggtgtacat ggcagcccgg agggggttcc
    13441 tctccccgcc ctcggacttc gtggagaggt actccgccga gcccatggag agggccgcgg
    13501 cgatcccggt gatgagaccg gccatggcga tgacccgggt gtcctggaga gcgagggtga
    13561 acccggccag ggtcccggtg agctccacga gggcgtcgtt caggccgagg acgatggccc
    13621 cggtgtactg gagccgccgc tcgtcgatga gcccgatgag ggccttctcg tgcccctctt
    13681 cgtcgaggag caccgcatcg aggccgggaa caaccgggag gaggtcccgg tacagcttct
    13741 gggccctttt ctccccacgc tccatcagtt tcagggagaa ggtgatcccg aagatgtggt
    13801 aaatgaggag gtagaaggcg atcctgatcc tgtcgggggc gatgtcggtg ccggtgaagg
    13861 accggaagaa cccgtaatga ccctgctcca ccgcggcgat ctcctcgagg accttccggt
    13921 tacccgcctc cgaggccttc agggcaaggg cccggtagat cccgtgctcg gtggtctcgc
    13981 ctctctggga tgcgaggacg gcccgcctcg ccttgtcggg gagattctgc ccctcgatca
    14041 tgttccctcc tccctggagg cgaggtacct cttcaccgag agctcgagga acttttcctc
    14101 acggtccacg ccgatgaatc tcctcccggt tcgggtgcag gcaaggccgg tggtggagct
    14161 cccggtgaag ggatcgagaa cgagatctcc ctttttcgag ctcgcgagga tgatccggag
    14221 gaggagctcg aggggcttct gggtcgggtg cctgccgaac cgtttctccc ccggtctcgg
    14281 ggccgggatg gaccagaccg accgcatctg ccggtggggc cgtttcaggg cgtccccggg
    14341 ccaggagccg ttcttcacga ggtcgtagtt gaaggtgtgc cggcactccg gacccttcgc
    14401 cgcccagagg agggtctcgt ggctcgccgt gaagtacctt cccgagaggt tggggggggc
    14461 gttcggcttg aaccagcaga tgtcgttcag gaggtggtag cccgcgatct ggagggcgta
    14521 gccgcaggcg tagatggagt ggtaggtccc cgaaatccag atgctccccg ccggggcaag
    14581 gacccgctgg caggcccgga tccaggcgag gtggccgcgg aagtcctcct cggtcccccg
    14641 gctctcgtcc cagcttccct tgttcaccgg ggccctcctc ccggcatggc aggtgaagcc
    14701 gtcgttggag agccggtagg gagggtctgc aaagatcagg tccaccgagt cctcagggag
    14761 gccctccagg aacccgaggg aatcggcatg gtaaagctcg aaaccatcgg cggcaaaata
    14821 gggcttcatc gctccacccc tcccttcgtc ctgacaggaa atatggggcg gttccctatt
    14881 tgagcatacc tttgacaggc cctggcatcg ggatcgaatc gccggtccgc ggctcgggac
    14941 ctgcccccgg atccggattc ggaaaaaccg gccaggcggg aggtaatatc aacaggcaat
    15001 tatgaaataa gtcaatttca tcaacaaata tatatatctt cgcataaaat actcccttaa
    15061 ccagatcccc catttcgggg aaaagttaag ctggattgaa tggtacgatg agacctgacg
    15121 gagagaacgg ggggcgggcg gctccggatc cggagatcgc cgtcctctat gtcgatgacg
    15181 agccggcgct cctcgagctc accacgctct tcctgggaaa ggctcacggg gatctcgcca
    15241 tcgataccgc atcctcggcc cgggttgccc tggagaagct caggctgcgc ccctacgatg
    15301 tcatcgtctc ggactacctg atgcagggca tggacggcat cgagttcctg aagggcctcc
    15361 gggccggggg gtgctccatc cccttcatca tcttcaccgg gaaggggagg gaggacgtcg
    15421 ccatcgaggc cctgaactcc ggcgcagact tctaccttca gaaggggggt gatccccggt
    15481 cccagttcgc ggagctcgcg aacatgatcc ggcaggccgc ccggaaacgg agagccgagg
    15541 aggcactcag ggcgcacgag gaacggctcc atgccatcat ccagggctcg cccattcccc
    15601 agttcgtcat cgggagggat caccgggtga tatactggaa ccgggcgctg gagcagaata
    15661 ccggcgttcc cgcgtccgag gtgctcggaa cgagggggca gtggaaggcc ttctatccct
    15721 tcgagaggcc ctgcctgtgc gatcttctcg tggacgggac ccccggggag atctcactct
    15781 ggtacgggga caaggccggc ccgtcccggc acgtcgaggg ggcctgcgag gtgaaggact
    15841 ttttcccgaa catgggcgac cacgggaggt ggctcctctt cacctccgtc gccatccggg
    15901 accggacggg cgccatcctc ggtgccctcg agaccctcga ggatatcacg gatcaggttt
    15961 ccgccgagga gaacctcagg gagagggaat gcgagctgga ggagacccgc gcccggctgg
    16021 atggcatctt ccaggcccac ccgggaacac ctgaccgggg gctgcacggg ggatccctcg
    16081 ggactgggaa cctccccgtc cccccggcag aacagtagcg acctctcccg gatcccggat
    16141 cctccggcag cgcgatcaac ccaggtccga tccggctggc aattcgtcaa ccagctcccg
    16201 tttattttcc ccgaaggaga gggggatagt tcacgaaggg aggttattca acattcccga
    16261 tttatataat tttctttctg aagggaaccc ggtaacaggt tatttcatta aaattataag
    16321 gccaataaaa ttcaaatgat tgaaataata atatagctgg ccggccaaac aaccctgcgg
    16381 gagaaactcc cggtggtgtg ggtaacccgt ggcatcttcc cccgatccct cgaggtttgg
    16441 cgaatacatg aagagggtgt cccatgtcat cgccctccag gggatcttcg gggccatcct
    16501 cctcctcacc cggtacctcc cggaccagtc ggtgcccctc ctcgcccggg gacaggaggc
    16561 cctcaccctc ctcgacgtga attccctcac cgtcttcgcc ctcttcctcg ttctctccat
    16621 cgccatcctc cggcaggagt ccgacttcgc gctcccgagg gaagacccct tcgtccgggt
    16681 cctcgaggga tacgggagcc cggcgggctc gaccaccctc ttccgggtgg tccatggcct
    16741 cttcacggtg gtcatgatcc tcgtgtacct cacggcgccg cccatcacgg cggtccccct
    16801 cgcccccacc ctccaccagg aggcgggggt cctcgtcctc ttcgtcatcg tggtcttctc
    16861 ctcccgggct gtctccttgg cggccatgcg gttctttgac cggaccatgg cgggccttcg
    16921 ccacgccttc gcctgcctta tctacctgct gggcctcggc ctcatcttcg gaggggccat
    16981 cacggggata gagaacctgc tcatcgcggg gatggcggtc atctccctcg agatcctcta
    17041 cgtcgtgatc cgggaggacc gccgggtccg ggccggggaa tcttcccgcg acggctgagg
    17101 cacccggggg accggttgcc gctgtacgcc ctgcgacggg gtattccccc gcatcatgaa
    17161 tacactatta agtagtccga aggagtacgc tagaaagaag gagcatggag gaacacccgg
    17221 tccaggacat gatgcggctc atggcctcga aggtgaggag catcgcaaac ggcatctccg
    17281 gcgaggaggt ggagggcatg atccgggaga tcctcgcggc aaaaagggtc tatgtcatgg
    17341 gggcggggag gtccgggctc gtcgccaggg cctttgccat gcgcctcatg cacctcggcc
    17401 tcacctcgta cgtggtcggg gagacggtga ccccggccgt gaacccggag gacctcgtga
    17461 tcgtcttctc cggttcaggg gaaacgagga ccgtcgccga tatcgcggag tccgcaaagg
    17521 ccatcggggc gaggctctgt ctgatcacga gcaagaggga ttcccggatc ggccggatcg
    17581 ccgactgctg cgtggtcatc gagagccagc gggacacgga gggggacgag cacgcggagt
    17641 tcgagatccg gcagatgatg ggcaagcaca agtcctttgc ccccctgggc accctcttcg
    17701 agaccgcctc ggtcatgttc gccgacgcgg tcatctcgcg gctcatggag atcaaggaga
    17761 tcaacgtcga gacgctcaag ggaaggcacg tgaacatcga gtgaagggag agaggggatg
    17821 gaagggatga ggttttccga gaaggtacag ggcatcgatc tctcaggcat ccggcggatc
    17881 ttcgaggc
//
LOCUS       DATGDI010000116         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_418542, whole genome shotgun sequence.
ACCESSION   DATGDI010000116 DATGDI010000000
VERSION     DATGDI010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418542"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..460
                     /locus_tag="VKO45_04545"
     CDS             119..460
                     /locus_tag="VKO45_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05178.1"
                     /translation="MAEYDLTDESSVGVLVRTRVKKYEEAHPGCSVFAADRRGMWDIR
                     FTCGEGRSISGTAYISAPEATGWVLKIHWPGGALGDIEKLDFTNTAGLEGLILKAIDW
                     EAGRERPRKKR"
     gene            635..1018
                     /locus_tag="VKO45_04550"
     CDS             635..1018
                     /locus_tag="VKO45_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMA05179.1"
                     /translation="MPNIGYFEIPADNVSRAKKFYTSLFGWTIGPTAMPGSMPPGMEY
                     QDVKCGDPVEGTLAMGGMYRRQMPMTPFTAYVVVEDYKKVKAKVEKLGGKILHEMEVE
                     GVGPTAVIQDTEGNVVGFWQPVMKK"
     gene            complement(1040..1576)
                     /locus_tag="VKO45_04555"
     CDS             complement(1040..1576)
                     /locus_tag="VKO45_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05180.1"
                     /translation="MADAAAKRSIDEVKADLAGFLADLDVEKVERYDEEGSWGFTMTF
                     GNYPILIDNEKGDPYCVVIFQVTLPEGEPVEKLNALYAKDDYKSLAAIFAAFSSPLTG
                     FSRIFEGEKVAGFTVSKYIYPFHPGFSIRELDGALQAVVSAGAMGVMFLKSLIGEWEE
                     EERAEKELGKSEPGPMYE"
     gene            complement(1628..>2186)
                     /locus_tag="VKO45_04560"
     CDS             complement(1628..>2186)
                     /locus_tag="VKO45_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMA05181.1"
                     /translation="PLEAALRVATSNFLVLEFIALSALFATTSVIMSSIMGGSRALFA
                     MARQRVIPGVFSRISGQGIPFLTVLVTGITIGAIVFLTNANLDWLASIFNFGTLVTFF
                     FINASLLRLRRTMPDVKRGFRVPLYPFTPALGMASCLLLSYYLSRNALLVGFGWIAIG
                     LVAYVLNRRRRGNLPEGTTTFPRET"
BASE COUNT          436 a          652 c          737 g          361 t
ORIGIN      
        1 aggaccggct ctggaagtac ctgggaggtc tcgcgggcct ggactgacgg ggccaggaac
       61 cgggagaggg ggaaataata taaccacgga ggaggcactc acagccggtg aggagaccat
      121 ggcggagtac gatctcacgg acgagtcatc ggtcggggtc cttgtccgga cccgggtgaa
      181 aaagtacgag gaggctcacc cggggtgttc ggtctttgcg gctgaccggc gggggatgtg
      241 ggacatccgg ttcacctgcg gggaggggag gtccatcagc gggaccgcct acatctcggc
      301 gcccgaggcg acggggtggg tgctcaagat ccactggccc ggcggagccc tcggggacat
      361 cgagaagctc gatttcacca acaccgcggg gctcgagggg ctgatcctca aagccatcga
      421 ctgggaggcc gggagggaga ggccccggaa gaagcgctga cgacccgctg agcatcgctc
      481 aaaccgtggg ttttcgcatg ccccgcctcc cttgccgcag ggagccggag catcgctcaa
      541 accgccgatt ctcgcatgcc cctgccgagg ggcatctctc aaattccggg tttcagggcg
      601 aaggttatat aggcggccgg cgtccgcctg tttcatgccc aacatcggct acttcgagat
      661 accggcagac aatgtttcgc gcgcgaagaa gttctacacc tccctcttcg ggtggaccat
      721 cgggccgacg gcgatgcccg gctccatgcc ccccgggatg gagtaccagg acgtgaaatg
      781 cggggacccc gtggagggga ccctcgccat gggcgggatg tacaggaggc agatgcccat
      841 gacccccttc accgcctatg tcgtggtgga ggactacaaa aaggtgaagg cgaaggtgga
      901 gaagctcggc gggaagatcc tccacgagat ggaggtcgag ggcgtgggcc ccacggcggt
      961 catccaggac accgagggaa acgtcgtcgg gttctggcag cccgtgatga agaaataatc
     1021 ctcctatttt tcgcgtccct cactcgtaca tcggccccgg ctcggacttc ccgagttcct
     1081 tctcggcccg ctcctcctcc tcccactcgc cgatgagaga cttgaggaac atgaccccca
     1141 tcgccccggc actcaccacc gcctggagcg ccccgtccag ctcccggatc gaaaagccgg
     1201 ggtggaaggg gtagatgtac ttcgacaccg tgaaacccgc gaccttctcc ccctcgaaga
     1261 tccgggagaa accggtgagc ggtgacgaga aggcggcgaa gatcgcggcg agggacttgt
     1321 agtcgtcctt cgcatacagg gcattcagct tctcgaccgg ctccccctcg ggaagggtga
     1381 cctggaagat cacgacgcaa tacgggtccc ctttctcgtt atcgatgagg atggggtagt
     1441 tcccgaaggt catcgtgaaa ccccacgagc cctcctcgtc gtacctctcg accttctcga
     1501 catcgaggtc cgcgaggaat cccgcgagat ctgcctttac ctcgtcgatg ctcctctttg
     1561 ccgccgcatc ggccatggga gggagtacgc ggggggcggt tatatgcatt gtgccccccg
     1621 ccctccttca cgtctcccgg ggaaacgtcg tggtaccctc cggtagattc ccgcggcgtc
     1681 tcctgttgag cacataggcg acgagcccga tggcgatcca cccgaacccg accaggaggg
     1741 cattccggct caggtaatac gagagaagga ggcagctcgc catcccgagg gcgggggtga
     1801 aggggtacag cggcacccgg aatccccttt tcacgtcggg catggtcctc cggagccgga
     1861 ggaggctcgc gttgatgaag aagaaggtga cgagggtccc gaagttgaag atggaggcga
     1921 gccagtcgag gttcgcgttc gtgagaaata cgatggcccc gatggtgatc ccggtgacaa
     1981 gcacggtgag gaaggggata ccctgcccgg agatgcggga aaagaccccg gggatcaccc
     2041 gctgccgtgc catggcaaag agggcccggg agccccccat gatggatgac atgatgaccg
     2101 aggtggtggc aaagagggcc gaaagggcga tgaattccag gacgaggaaa ttcgaggtgg
     2161 cgacccggag ggcggcctcg aggggg
//
LOCUS       DATGDI010000117         3996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_389961, whole genome shotgun sequence.
ACCESSION   DATGDI010000117 DATGDI010000000
VERSION     DATGDI010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389961"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="VKO45_04565"
     CDS             <1..160
                     /locus_tag="VKO45_04565"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HMA05182.1"
                     /translation="PYEACETTYRRLFAWIGEQGLSITGPIRETYLNDPREVQPDQIL
                     TEIMAPVA"
     gene            complement(254..949)
                     /locus_tag="VKO45_04570"
     CDS             complement(254..949)
                     /locus_tag="VKO45_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMA05183.1"
                     /translation="MEWATRLQDVIERAPGVKSFRFPRPDEFQYDPGQWMFITIGKGG
                     EMTKHFTISSSPTEGEFIEFTKKITDHEFSLALDRMRAGDPVTIDGPYGEFTFKGEYP
                     GIGMLTGGIGITPFRSMIRYCTDKQIPSRITLLYGNHNEESIVFRRELEEMGSRNPNL
                     AIRHCLSHPGEDWAGRRGHVDRAVIEEEIPDYLERVFYVCGPPALVTGLVGALGTLQV
                     PPSQVKVEDFPGY"
     gene            1167..1709
                     /locus_tag="VKO45_04575"
     CDS             1167..1709
                     /locus_tag="VKO45_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HMA05184.1"
                     /translation="MKELRIHGRGGQGSVTAAELIATAAFEGGVFAQAFPMFGVERRG
                     APVQAFVRFADKKIRLRSQIYEPDYIIVQDSTLIRDVNVFAGMKKGGIAIVNTSKAGG
                     WSSPEGVRVITVDATGIALEVLGIPITNTSLMGAFAAASGEITMEAIEHAIRNRFKEE
                     IAVKNIEAARRAYSLVKGAA"
     gene            1710..1970
                     /locus_tag="VKO45_04580"
     CDS             1710..1970
                     /locus_tag="VKO45_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA05185.1"
                     /translation="MALSVGCTARPGKARGNKTGAWRVFKPVFHHNKCTKCGICSMVC
                     PEGCILEQEDEAFLPDYDYCKGCGLCAEECPAEDIEMVKEEK"
     gene            1972..3126
                     /gene="porA"
                     /locus_tag="VKO45_04585"
     CDS             1972..3126
                     /gene="porA"
                     /locus_tag="VKO45_04585"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorA"
                     /protein_id="HMA05186.1"
                     /translation="MMRFIEGSIAVAEAVRMCRPDVVSAYPITPQTHIVEALAEMVAN
                     CTLDAEYITVESEFSAISACLGAVAAGSRAYSATTSQGLFLMFEPCFNFAGMRLPMVM
                     TIANRAVSAPLSIWNDQQDSISLRDSGWLQFYAEDNQEAADLHYLAYKVAEDHGVLLP
                     AMVCFDGFILSHTYEPVDMPTQEQVDAYLPRFTPIQRLDAQDPITFGMFAFPEYYMEF
                     RYAVDQAMQRAKEKIREAGKELSAKIGRDYSAFVEGYRMEDAETVLVAMGSLCGTVKD
                     AIDEMRESGKKVGLLKIRTYRPFPGEEVLRALSGAKAVAVLDKNISLGSKGAVGLEVK
                     DALYGSGIPVHDYIVGLGGRDLRKRDIARIADLAEKGEGDLFYGLREGVA"
     gene            3127..>3996
                     /locus_tag="VKO45_04590"
     CDS             3127..>3996
                     /locus_tag="VKO45_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMA05187.1"
                     /translation="MVIDKSLELFDCGHRACGGCGPALAARLILKAAGKNTIVVTATG
                     CLEVFSSPYPETSWKVPWVHSLFENAAAVGSGVEAALRKQGRSEKVVIIGGDGATFDI
                     GMLCISGAFERGHDFTYICYDNEAYMNTGIQRSGATPLDASTTTSPAGKCSTGNRRPK
                     KDMPAILAAHGAPYVATTSIAYPTDLMQKVERAIETKGPCYVQILAPCPTGWGYEGDR
                     TMVIGKLAVETGLFVLFEQTDGTVTKVKKVMRKPVEEYLKVQKRFAHLFKPQRNETEI
                     ARIQAIADANAAKF"
BASE COUNT          738 a         1262 c         1281 g          715 t
ORIGIN      
        1 gccctacgag gcctgtgaga ccacctaccg acgcctcttt gcctggatag gagagcaggg
       61 gctcagcatc accggtccca tccgggaaac gtacctgaac gatccgcgag aagtacaacc
      121 cgaccagatc cttaccgaga tcatggcgcc ggtcgcatga ggtgactgta gaatcgtggg
      181 agaagagggt ttcgatggaa gggcgtaccc gcaggggaca gaacgggtcg gacctgttcg
      241 ccgcccgtcg gactcaatat cccgggaagt cttcgacctt tacctgtgac gggggcacct
      301 ggagggtgcc gagcgcgccc acgaggccgg tgacgagggc aggcggtccg cagacgtaga
      361 acacccgttc gaggtaatca ggtatttcct cctcgatgac ggccctgtcc acatggcccc
      421 gcctccccgc ccagtcctcg ccgggatggg agaggcagtg acggatggcc aggttggggt
      481 tccgggaccc catctcctcc agttcccggc ggaatacgat gctctcctcg ttatggttcc
      541 cgtagaggag ggtaatcctg gatgggatct gtttgtcggt gcagtacctg atcatgctcc
      601 tgaagggtgt gatgccgata cccccggtga gcatgcctat cccggggtat tcgcccttga
      661 aggtgaattc accgtacggt ccgtcaatgg tcacggggtc ccctgcccgc atcctgtcga
      721 gggccaggga gaattcatgg tcggtgatct ttttcgtgaa ttcgatgaat tcaccctcgg
      781 tcgggctgct ggatatggtg aaatgcttgg tcatctcccc gcctttcccg atggtgatga
      841 acatccactg gcccgggtcg tattgaaact cgtcaggccg ggggaaccgg aagctcttca
      901 caccgggtgc cctctctatg acgtcctgca gcctggtggc ccattccatc ccattcacct
      961 cggtttccct gataggtgat ccgaggaatc tccatctccc ctctttttat ttaacccttg
     1021 gggcgccttc tcgccgctga aaataccatg catatccgcg cccttcccat gtgatacgga
     1081 cgattttacg cccgttctga ttcaatcgga gctcaaaaga gatacattta tccacgatta
     1141 atcatgtaaa atgtaccggg attttcgtga aggagttgcg catccacggg aggggcggcc
     1201 agggctcggt cacggcggcg gagctcatcg ccacggcggc cttcgagggc ggggtctttg
     1261 cccaggcctt ccccatgttc ggggtcgagc ggaggggggc gccggtccag gcctttgtcc
     1321 ggttcgcgga taaaaagatc cggctcagga gccagatcta cgagccggac tacatcatcg
     1381 tccaggacag cacccttatc agggacgtga atgtctttgc cgggatgaag aagggcggga
     1441 tcgccatcgt aaacacctcg aaggcgggtg gctggtcttc ccccgagggc gtccgggtca
     1501 tcaccgtgga tgccacgggg attgccctgg aggttctcgg gatccccatc acgaacacga
     1561 gcctcatggg ggcctttgcc gcggcgagcg gggagatcac gatggaggcc atcgagcacg
     1621 ccatccggaa ccggttcaag gaggagatcg ccgtaaagaa catcgaggcg gcgcggcggg
     1681 cgtacagcct cgtgaagggg gcggcctgaa tggccctctc tgtcggttgc accgcgagac
     1741 cgggaaaggc ccgggggaac aagaccgggg cctggcgcgt tttcaagccg gtctttcacc
     1801 acaacaagtg cacgaagtgc gggatctgct ccatggtctg ccccgagggg tgcatcctcg
     1861 agcaggagga cgaggccttc ctcccggact acgactactg caagggctgc ggcctctgcg
     1921 ccgaggagtg cccggcagag gacatcgaga tggtgaagga ggagaagtga catgatgcgc
     1981 ttcatcgagg gctccatcgc ggtcgcggag gcggtccgca tgtgccggcc cgacgtggtc
     2041 tcggcctacc ccatcacccc ccagacccac atcgtcgagg ccctcgccga gatggtggcg
     2101 aactgcaccc ttgacgccga gtacatcacc gtggagtcgg agttctcggc catctcggcc
     2161 tgcctcggcg ccgtggcggc gggctcccgg gcctactcgg ccaccacctc ccaggggctt
     2221 ttcctcatgt tcgagccctg cttcaacttc gccgggatgc gcctccccat ggtgatgacc
     2281 atcgcgaacc gcgccgtgtc ggcccctctc tccatctgga acgatcagca ggactccatc
     2341 tccctccggg actccggctg gctccagttt tacgccgagg acaaccagga ggcggccgac
     2401 cttcactacc tggcctacaa ggtcgccgag gaccacgggg ttctcctccc tgccatggtc
     2461 tgcttcgacg ggttcattct ctcccacacc tacgagcccg tggacatgcc cacccaggag
     2521 caggtggatg cgtacctgcc ccggttcacc cccatccagc ggctggacgc gcaggacccc
     2581 atcaccttcg ggatgtttgc ctttcccgag tactacatgg agttccggta cgccgtggac
     2641 caggccatgc agagggcgaa ggagaagatc cgggaggcgg gaaaggagct ctcggcgaag
     2701 atcggccggg actacagcgc ctttgtcgag ggctaccgga tggaggatgc cgagaccgta
     2761 ctcgtcgcca tgggctccct gtgcggcacg gtcaaggatg ccatcgacga gatgcgtgaa
     2821 tcggggaaga aggtcgggct cctcaagatc cgcacctacc ggccattccc cggcgaggag
     2881 gtccttcggg ccctctcggg ggcaaaggcg gtcgcggtcc tcgacaagaa catctccctc
     2941 gggtccaagg gcgcggtggg actcgaggtg aaggatgccc tctatgggtc cgggattccg
     3001 gtccatgact acatcgtcgg gctcgggggc agggacctcc ggaagcggga catcgcccgg
     3061 atcgcggacc tcgccgagaa gggcgagggc gatctcttct acggccttcg cgagggggtg
     3121 gcataggtgg tcatcgacaa gagcctggag ctcttcgact gcggccaccg ggcctgcggc
     3181 ggatgcggcc cggcccttgc cgcccggctc atcctgaagg cggcggggaa gaacaccatc
     3241 gtcgtgacgg cgaccgggtg cctggaggtc ttctcgagcc cctacccgga gacctcctgg
     3301 aaggtcccct gggtccactc cctcttcgag aacgcggcgg cggtgggctc cggggtcgag
     3361 gcggcgctca ggaagcaggg cagaagcgag aaggtggtga tcatcggggg cgacggggcg
     3421 accttcgaca tcgggatgct ctgcatcagc ggggccttcg agcgtggcca tgacttcacc
     3481 tacatctgct acgacaacga ggcctacatg aacaccggca tccagcgctc gggcgcgact
     3541 cccctcgacg cgagcaccac gacctcccct gccgggaagt gctccacggg gaaccggcgg
     3601 cccaagaagg acatgccggc catcctcgcc gcccacggcg ccccgtacgt cgccaccacc
     3661 tccatcgcct atcctaccga cctgatgcag aaggtggagc gggccatcga gaccaagggg
     3721 ccctgctacg tccagatcct ggccccatgc cccaccgggt ggggctatga gggcgaccgg
     3781 accatggtca tcggaaagct cgcggtggag acgggactct tcgtcctctt cgagcagaca
     3841 gacggaacgg tcaccaaagt aaagaaggtg atgcgaaagc ccgtcgagga gtacctgaag
     3901 gtccagaagc ggttcgccca cctcttcaag ccccagcgga acgagaccga gatcgcccgg
     3961 atccaggcca tcgccgatgc gaacgcggcg aagttc
//
LOCUS       DATGDI010000118         1950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_215542, whole genome shotgun sequence.
ACCESSION   DATGDI010000118 DATGDI010000000
VERSION     DATGDI010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215542"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1950
                     /locus_tag="VKO45_04595"
     CDS             <1..>1950
                     /locus_tag="VKO45_04595"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HMA05188.1"
                     /translation="VHDQIREIVEEEAPQPGKKHPAKKQPDDAQAWYGRIHRSVLSGY
                     LSNIAVKKEKNTYGAAGGRQVVVFPGSGLYNRGGDWIVAAEIVETTQLFARTAANIDR
                     EWLEALGGSLCRSTTLNARWDRKRGEVTALERVSLYGLTIVEVRRISYGRIDPQEATR
                     IFIREALVPAEMERPLPFLAHNRDLIERVRDMENRLRRRTFLTTDDVLAAFYEERLQD
                     IYDVRTLQRMVRDRGSDSFLRMTEEDVLRQAPDPAELSLYPDTVSAGRLALPLSYRFR
                     PGETDDGVTLKLPASALTRLRPEAVEWAVPGLLQERVTALVKALPRDIRRHLIPIPQS
                     VETILREMKRGEGSLPAALSECLKRKFWLDVPLAAWDLGGLPDHLKLRYAVVDPKGKE
                     LRSTRDLESLRKDRLEEEESRAFSRARAAWERTGIARWEFPDLPEEIPEEIRLDAQNV
                     LEGVAYPALVPAGEAVDLRLMTNRREAETAHRAGVKTLLTLHFRKDLELARRYLALRG
                     EMKVWSAYFGGTKVLEEALFQGLVRRLLEKDIRTRQDFEAYTRLMGPTLLKAPQELLG
                     EVESVLRAYHETRQALAGLEAANLSNRTVLNFLEEMRTELQRLLPLSFLEIYDRERIP
                     QVIRYLKALQVRAERGAVHIDKDRG"
BASE COUNT          366 a          654 c          624 g          306 t
ORIGIN      
        1 acgtccacga ccagatacgg gagattgtcg aggaggaggc cccccagccc gggaagaagc
       61 accccgcaaa gaagcaaccc gacgacgccc aggcctggta tggccggata caccgctcgg
      121 tgctgagcgg ctacctatcg aacatcgccg tgaaaaagga gaaaaacacc tacggcgccg
      181 caggcgggcg ccaggtcgtg gtcttccccg gctcgggtct ctacaaccgc ggcggagact
      241 ggatcgtggc agcggagatc gtggaaacga cacagctttt tgcccgcacg gccgccaaca
      301 tcgacaggga gtggctcgag gccctgggcg gaagcctctg ccgctcgaca accctgaatg
      361 cccgctggga ccgcaagcgc ggcgaggtga cggccctcga acgggtgagc ctctacggac
      421 tgacgatcgt cgaggtaagg cgcatctcct acggccgcat cgatccgcag gaggccaccc
      481 ggatcttcat ccgcgaggcc ctcgtgccgg cagagatgga gaggcccctg ccctttcttg
      541 cccacaaccg cgacctcatc gagcgggtcc gggacatgga aaaccggctc cgcagaagga
      601 catttttaac cacggacgac gtcctggccg ccttctacga ggagcggctc caggacatct
      661 atgacgtccg gactctccag agaatggtca gagaccgggg gagcgattcg tttctgagga
      721 tgaccgagga ggacgttctc cgtcaggctc cggacccggc ggagctttcc ctttacccgg
      781 acaccgtttc agcgggcagg ctggcccttc cgctttctta ccggttcagg ccgggagaaa
      841 ccgacgacgg ggttaccctg aagctcccgg cgagcgcgct cacgcgcctg cggcccgagg
      901 ccgtcgagtg ggccgtcccg ggtcttttgc aggaacgggt cacggcactc gtcaaggccc
      961 tgccccggga catccgcagg cacctcattc ccatcccgca gtcggtggaa acgatcctgc
     1021 gggagatgaa gcgcggcgaa gggtctctgc ccgcggccct ctcggagtgc ctgaagcgca
     1081 agttctggct ggacgtcccc cttgcggcct gggacctggg ggggctgccc gatcacctga
     1141 agctccgcta cgccgtcgtg gacccgaagg gcaaagaact ccgctcgacg cgcgacctcg
     1201 aatccctgag aaaagaccgt ctcgaagagg aggaatcccg ggccttcagc cgggccaggg
     1261 cggcctggga acggacaggg atcgcccgct gggagtttcc cgatctgccc gaggagatcc
     1321 ccgaggaaat ccggctggat gcgcaaaacg tcctcgaggg ggtcgcctac ccggcccttg
     1381 tccccgccgg cgaggccgtc gaccttcggc tcatgacaaa ccgcagggag gccgaaacag
     1441 cccaccgggc cggcgtaaag acgctcctga cccttcattt ccggaaagat ctggaactcg
     1501 cccgccgtta ccttgccctc cggggagaga tgaaggtctg gtccgcctat ttcggcggga
     1561 cgaaggtgct ggaggaggcc ctcttccagg gtcttgtccg ccgcctcctg gaaaaggaca
     1621 tccggacccg gcaggacttc gaagcgtata cccgcctgat ggggccgacg cttctcaagg
     1681 caccccagga gcttctcggg gaagtggagt ccgttcttcg cgcctaccac gagactcgcc
     1741 aggccctggc ggggctcgag gcggcaaacc tctcgaaccg cactgttctc aatttcctcg
     1801 aggagatgcg caccgagctt cagcgcctcc tgcccctgtc ttttctcgag atctacgacc
     1861 gggagcgcat cccccaggtg atccgctacc tcaaggcgct ccaggtccgt gccgagcggg
     1921 gcgccgtgca catcgacaag gaccggggac
//
LOCUS       DATGDI010000119         1546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_331709, whole genome shotgun sequence.
ACCESSION   DATGDI010000119 DATGDI010000000
VERSION     DATGDI010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331709"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1546
                     /gene="mrdA"
                     /locus_tag="VKO45_04600"
     CDS             <1..>1546
                     /gene="mrdA"
                     /locus_tag="VKO45_04600"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HMA05189.1"
                     /translation="DVKDLQGLVGKMETLYAKVNLTFPMDYETILENRRPFTPLRVDR
                     NVSREKLALVETHSLDLPGVGIDVMPVREYYYGESMAHIIGYTGEVSRDDLERDKSAG
                     YKSGDLIGKFGLEKALDPFIRGKAGGEQVEVNVIGKKVKTLGRVEPSQGYRVVLSLDS
                     QVQKAAWTALEGKAGAVVALDPRDGSILALVSRPGFDPNQFNRGITSDLWEKLSTNPL
                     HPMENRAVAGQYPPGSTYKLIVAAAALEEGIITPETAFNCPGHFEMGTRTFRCWRKQG
                     HGRVSLHRAIVESCDVYFYNVGKLLGVDRLAEYAQAFGLGTRTGVALVGERRGLIPTR
                     EWKLARFGVPWQPGETISIAIGQGYNTATPIQLANAYAAVANNGEYFTPRIVKRVETD
                     DGEIIEEFRPEKKAVLPVSQENIQLLKKALWGVVNEPGGTGGQARVAGRDVGGKTGTA
                     QVIGMAEGDDGSSYPYEYRDHALFVCFAPRDNPEIVVAVVVEHAGHGGSVAAPVARKV
                     LEAYFAG"
BASE COUNT          307 a          454 c          534 g          251 t
ORIGIN      
        1 agatgtgaag gacctccagg ggcttgtggg gaagatggag accctctatg caaaggtgaa
       61 cctgaccttt cccatggact atgaaaccat cctcgagaat cgcaggccct tcaccccgct
      121 gcgggtcgac cgcaacgtga gcagggaaaa actggccctc gtcgagaccc attcgctgga
      181 tctgcccggc gtcgggatcg acgtcatgcc tgtgagggag tattactacg gggagtcgat
      241 ggcccacatc atcggataca caggcgaagt gagccgtgac gatctggagc gcgacaaatc
      301 agccggctac aagagcggcg atctgatcgg gaagttcggc ctggaaaagg ccctcgaccc
      361 attcatccgg ggcaaggcgg gcggggagca ggtcgaggtc aacgtcatcg gcaagaaggt
      421 caagaccctg gggcgggtcg agccctcaca gggctaccgg gttgttctct ccctggattc
      481 gcaggtccag aaggcggcct ggacggcttt ggagggaaag gcgggggccg tcgtggctct
      541 cgatccccgg gacgggtcca tcctcgcgct ggtgagccgg ccgggattcg accccaacca
      601 gttcaaccgg gggatcacgt cggatctgtg ggagaaactc tccacgaatc ccctgcatcc
      661 catggaaaac agggccgttg cgggccagta tccgcccggt tccacctaca agctcatcgt
      721 ggcagcggcg gccctggagg aagggatcat cacgcccgag acggccttca actgcccggg
      781 gcatttcgaa atggggacgc gcaccttccg ctgctggcgc aaacagggac atggccgggt
      841 gagcctgcac cgggccatcg tcgagtcctg tgacgtctac ttctacaacg tggggaagct
      901 tctcggtgtg gaccggctcg ccgagtatgc ccaggccttc gggctgggga cccggacagg
      961 ggtcgccctg gtcggcgaac gaagggggct catcccgacg agggaatgga aacttgcccg
     1021 gttcggtgtt ccctggcagc ccggcgagac gatctcgatt gccatcggcc agggctacaa
     1081 cacggcgacc ccgatccagc tggccaacgc ctatgcggcc gttgcaaaca acggggagta
     1141 cttcaccccg aggatcgtga aacgtgtaga gaccgatgac ggggagatca tcgaagaatt
     1201 ccgtcccgag aaaaaggccg tgctccccgt gagccaggaa aatatccagc tcctgaagaa
     1261 agcgctgtgg ggcgtggtga atgagccggg gggcacggga ggccaggccc gtgtggccgg
     1321 cagggacgtg ggcggcaaga cgggaacggc gcaggtgatc gggatggccg agggggacga
     1381 cggctcgtct tacccctacg agtacaggga ccatgccctc ttcgtctgct ttgcgccccg
     1441 ggacaacccg gagatcgtcg tcgccgtggt ggttgagcac gcgggccacg gcggttcggt
     1501 ggcagcgccg gtggccagaa aggtcctcga agcctacttt gcgggg
//
LOCUS       DATGDI010000120         5332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_41098, whole genome shotgun sequence.
ACCESSION   DATGDI010000120 DATGDI010000000
VERSION     DATGDI010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41098"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VKO45_04605"
     CDS             <1..436
                     /locus_tag="VKO45_04605"
                     /inference="COORDINATES: protein motif:HMM:NF021370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HMA05190.1"
                     /translation="QALVIEAGGQKTAYVLLDGNNIVKGGREVLLGGIRDLVDDCEVM
                     TSDTHLVNTITGKNPVGLGVPPSDIAPYVREAVEIALADLAPAETGAATAWCRNVQVF
                     GPQRISQIASISTTTVNFIGPLGISLLLSAYLLTLVTYMALV"
     gene            788..872
                     /locus_tag="VKO45_04610"
     tRNA            788..872
                     /locus_tag="VKO45_04610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:824..826,aa:Leu,seq:taa)
     gene            complement(899..2365)
                     /locus_tag="VKO45_04615"
     CDS             complement(899..2365)
                     /locus_tag="VKO45_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HMA05191.1"
                     /translation="MKNRRTVDLEAIAWHAMEKYGFEPGFPEAVETEVATISGEKPQG
                     ESDGTRDLRGLLWSSIDNEDSMDLDQIEYCERRGNGEIHVRIAVADVDLYVKKESPAD
                     GHAAHNGTSVYTGIRTFPLFPDRLSKGITSLLPGEDRTAIVVEYAVLFDGSFRPGEVY
                     PALVNNKAKLVYEVIGDWFEGTTPVPEVVRDVPGLEAQLRLQLEASMLLKKYRMEQGA
                     LELDTLEPKVVVEGGKVSRLVAPKKNPAGHLIEDFMIAANGTMVARLEEAGIPLVQRV
                     VRVPKHWDGIVETAAMYHERLPEEPDAKALSEFLVRRKKADPERFPDLSLTIVKLLGR
                     GEYLALVPGQPPMGHFGLAVTHYTHSTAPNRRFVDLIIQRLVKSVLAGAPSPYEAGEL
                     TGICAWLTDREHEANKVERFMRKASAALLLSDRIGESFDAIVTGVIEHGIFVRLFDPP
                     AEGKVVRRERGLKVGQFVRVRLLSTDPYNGFIDFERVG"
     gene            complement(2567..3019)
                     /locus_tag="VKO45_04620"
     CDS             complement(2567..3019)
                     /locus_tag="VKO45_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05192.1"
                     /translation="MEPDTGRAGDDYASYRILVDLWAAENPVKTAKLLALLVVHAVLI
                     VAMTLGGGLVPRNWPVCLAGVAFSLVFVLSLGRTLLFQEAWRLKIRALASRYIGEERF
                     QVLETAEAQRDAPPLLRILGGIPSVYYLLGAPLLLLASWLVALVLLLS"
     gene            complement(3098..3982)
                     /locus_tag="VKO45_04625"
     CDS             complement(3098..3982)
                     /locus_tag="VKO45_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05193.1"
                     /translation="MTGSSARVAALLILSLVVLMVPPVLGFEATIEPATIQDRNPISI
                     NLSGVTDGVSLNVTLTATFTPPPGVAWLNLTNWNYPFALQGGRMIAGGRNVNEMTFLV
                     REGNTFMTQRGSGAGTITVEIPLDVQPMATTDFSIAYKLHDNTEPVTLTLVQQGSKKG
                     LEQDAVLTPSILGIVEGNLSVQVRFNGTLVGARDIQVLEEAPLPPTPEVNVTATPMST
                     PVSTATSTPTPSHVPTTPATTQTTSPPVATKPVTTPPATEVPVPDTPSAGPSPFILAY
                     GAVIVIIALLADYYLLKD"
     gene            4100..4594
                     /locus_tag="VKO45_04630"
     CDS             4100..4594
                     /locus_tag="VKO45_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05194.1"
                     /translation="MKGLRMVTLLLILGALAIPPVAALHVRYLDIAVDRDGSATVTFE
                     YTLPWIEQVFAFLGVVSPDRDFGRILGVASGGEIVALSAGDGMTTFSMRGFANVTGTP
                     PDTTCTTPALNLSWGQAGYEASILAPLLSPDFSPDVTVVRFPDAYSLTFRDELLIPGI
                     THAC"
     gene            complement(4578..>5332)
                     /locus_tag="VKO45_04635"
     CDS             complement(4578..>5332)
                     /locus_tag="VKO45_04635"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HMA05195.1"
                     /translation="FRTNSYEYSPTIPLDAEKAILRKAAEWERALLRDPAGFLASLET
                     PVKAAPTGGTTDALIIQGSPRPDGNCSILAGWAAEEAAGIGKRARVVYLDDLVIHPCI
                     GCYQCYNTGSCTFEDDMAELIRALRSAPVLVICTPVYTNTVPGGLKIFIDRCQAYHAE
                     KNLFYPGGEKKGILLSVAGRTGMANFTCVKKVVVSFMRNLGIRVAGEVLIDRIDGIRD
                     VRTLPGLREEIGDLVREAFSDLKKEPVLSRRG"
BASE COUNT          956 a         1668 c         1746 g          962 t
ORIGIN      
        1 gcaggccctc gtcatcgagg cgggggggca gaagaccgcg tacgtcctcc tggacgggaa
       61 caacatcgtg aagggcggac gggaggtgct cctcggcggg atccgggacc tcgtggacga
      121 ctgcgaggtg atgacctccg atacccacct cgtgaatacc atcacgggca agaaccccgt
      181 cggcctgggg gtcccgccat ctgatatcgc gccctacgtc cgggaggccg tggaaatcgc
      241 cctcgcggac ctggcgcctg ccgagacggg ggccgcgacg gcatggtgca gaaacgtcca
      301 ggtcttcggc ccccagagga tttcccagat cgcctccatc agcacgacca ccgtgaactt
      361 catcggtcct ctcgggatct ccctcctcct ctccgcctac ctcctgaccc tcgtcaccta
      421 catggcactc gtgtaggaaa atatggtcac agaaaatgcc aaccggcgaa tgttccatgg
      481 actccgtcga ttaaataccg ggcatctcaa actatccctg atccagcctc aatccctgac
      541 tgattgatgc tcccggggga ctcgcttcgc tcgccccctg ccgctgtggc gacccccaga
      601 ttgcgatggc ccgatgattc cgtgcccgtg ggaacaggaa ttaccggaaa ccggggggtg
      661 cccgtgcggc ggggtggcgt agccccccga gagcacccca tgaaaaaaag agagtctcaa
      721 aattcttctg tagaatctgc ccagagtggg cgcaaccctt tttcagatag agatacaagc
      781 ttcccgtgcg agggtaacca agccaggcca acggtgttgg acttaagatc caatctcgca
      841 ggagttcgtg ggttcgaatc cctcccctcg catccctgat aggggaaatt ttccctcctc
      901 accccacccg ctcgaagtcg atgaacccgt tgtacggatc ggtgctgagc agccgtaccc
      961 ggacaaactg gccgaccttg agaccgcgtt ccctccgcac gacctttccc tcggccggag
     1021 ggtcgaagag acggacaaag atcccgtgct cgataacgcc cgtcacgatg gcatcgaagc
     1081 tctcgccgat gcggtccgag aggaggaggg cggcgctcgc cttgcgcatg aaccgctcga
     1141 ccttgttcgc ctcgtgttcc cggtccgtga gccatgcgca gatcccggtg agctccccgg
     1201 cctcgtacgg gctcggggcg ccggcgagca ccgatttcac gagccgctgg atgatgaggt
     1261 cgacaaacct ccggttcggg gcggtggagt gggtgtagtg ggtgacggca aggccgaagt
     1321 ggcccatggg cggctgcccg ggaaccaggg ccaggtactc cccccggccc aggagcttca
     1381 cgatggtaag agagaggtcg gggaaccgct ccggatcggc cttcttccgc cggacgagga
     1441 actccgacag ggccttcgca tccggctcct ccgggagccg ctcgtggtac atcgcggcgg
     1501 tctccacgat cccgtcccag tgcttcggga cccggaccac ccgctggacg agaggaattc
     1561 cggcctcttc gagccgggcg accatggtcc cgttcgcggc gatcatgaag tcctcgatga
     1621 ggtggcccgc ggggttcttt ttcggggcca ccagccgcga gaccttcccc ccctcgacga
     1681 ccaccttcgg ctccagggta tcgagctcga gggccccctg ctccatccgg tacttcttga
     1741 ggagcatcga ggcctcgagc tgcagcctga gctgggcttc caggcccgga acgtcccgga
     1801 cgacctcggg gaccggcgtc gttccctcga accagtcccc gatcacctcg tacacgagct
     1861 ttgccttgtt gtttacgagg gccgggtaca cctcccccgg gcggaaggaa ccgtcaaaaa
     1921 ggacggcgta ctcgacgacg atggccgtcc ggtcctcccc ggggaggagg gaggtgatac
     1981 ccttcgagag ccggtcggga aagaggggaa aggtccggat ccccgtgtag accgaggtcc
     2041 cgttgtgggc cgcgtgcccg tccgccgggg actccttctt cacgtagaga tcgacgtccg
     2101 cgacggcgat cctgacatgg atctccccgt ttcccctgcg ttcgcagtac tcgatctggt
     2161 cgaggtccat ggagtcctcg ttgtcgatgg acgaccagag gaggccgcga agatcccggg
     2221 ttccgtcact ctccccttgc ggcttttccc cggagatggt ggcgacctca gtttccacgg
     2281 cctcggggaa gcctggctca aagccgtact tctccatggc atgccaggcg atggcctcga
     2341 gatcgactgt cctccggttc ttcattacct tccgctccgg gaattaccat ccccgggcat
     2401 atagcccttc aggttcttgg ttctcctggt ggaggtcgac tccgctctct ggatctccct
     2461 cgtccccatc gccgtcaccg gaaacccttc ctggttccgg gggaggagac ccgggaaccg
     2521 ggagcaccct gacctggaga gggtgcgacg ggacgagatc cttggttcag gaaaggagga
     2581 ggacgagcgc gacgagccag gaggccagga ggaggagggg cgccccgagg aggtaataca
     2641 ccgacgggat ccctcccagg atcctcagga ggggcggggc atccctctgc gcctcagccg
     2701 tttcgagtac ctggaaccgc tcctcgccga tatatcgtga tgcaagggca cggattttga
     2761 gccgccacgc ctcctggaag aggagggtcc tccccaggga gaggacgaag acaagggaaa
     2821 aggcgacacc ggcaaggcag accggccagt tccgggggac caacccgccc ccgagggtca
     2881 tggccacgat gagcacggca tggacgacaa ggagagcgag aagcttcgcg gtcttgacag
     2941 gattctcggc ggcccagagg tccacgagga tacggtagga ggcatagtcg tctccggccc
     3001 ggcccgtatc cggttccatg aggatgagtc tccggggctc gagataatgg ctctgtcccc
     3061 caccgggatg tgagagggga tggcggggcg ggttctccta gtccttcagg aggtagtaat
     3121 ccgcgaggag cgcgatgatg acgatgaccg ccccataggc gaggatgaag ggcgacggcc
     3181 cggccgaggg ggtatcggga accggcacct cggtggcggg aggggtcgtc accggcttgg
     3241 tggccaccgg gggagaagta gtctgggtgg tggccggagt ggtgggtaca tgggagggag
     3301 tgggagtgga agtagcggtg gacacggggg tggacatggg ggtggcggtg acattcacct
     3361 ccggtgttgg gggaaggggg gcctcctcca ggacctggat atcccgggcg ccaacgaggg
     3421 tgccattgaa gcggacctga acggagaggt tcccctccac gatgcccagg atggacgggg
     3481 tgaggacggc atcctgctcc aggcccttct tgctcccctg ctggacgagg gtgagggtga
     3541 cgggttcggt attgtcgtgc agcttgtagg cgatggagaa atcggtggtc gccatgggct
     3601 ggacgtccag gggaatttca accgtgatgg tccctgcccc tgatccgcgt tgtgtcatga
     3661 aggtgtttcc ctcccggacg aggaaggtca tctcgttcac attcctgccg cccgcgatca
     3721 tcctgcctcc ctgcagggcg aaggggtagt tccagttggt gagattgagc caggcgaccc
     3781 cggggggagg cgtgaaggtg gcggtcaggg tcacattcag ggaaaccccg tccgtgacac
     3841 cggaaagatt gatggaaatc gggttcctgt cctggatcgt ggcaggctcg atggtcgctt
     3901 caaacccgag aacagggggg accatgagga ccaccaggga aaggatgagg agggcggcca
     3961 cccgggcgga ggatccagtc atactcatcg gaataacgat ttttaggagg cgtgaaggca
     4021 tgaacctgcc gattggtccc ggggcgtccc cgggagaaag attatggagc ccggttccca
     4081 aactggtggt ggtgaatcaa tgaaagggct ccggatggtc actctcctcc tcatcctcgg
     4141 ggccctcgcc atcccgccgg tcgcagccct ccatgtgagg tacctggata tcgccgtcga
     4201 ccgggatggg agtgccaccg tcaccttcga gtacaccctc ccctggatcg aacaggtctt
     4261 cgccttcctc ggggtagtga gccccgaccg ggatttcggc cggatcctcg gggtggcctc
     4321 gggcggagag attgtcgccc tctcggcggg tgacgggatg accaccttct cgatgagggg
     4381 ctttgcaaac gtcacgggga cgccccccga caccacctgc accacgcccg ccctcaacct
     4441 gagctggggg caggccggat acgaggcctc gatcctcgcg ccgctcctct ccccggattt
     4501 ctcgcccgat gtaaccgtgg tccggtttcc tgatgcctat tccctgacct tccgggacga
     4561 gctcctgatc cccggcatca cccacgcctg ctgaggacgg gttccttttt caggtctgaa
     4621 aaggcctccc tcaccaggtc gccgatctcc tcacggaggc cggggagagt ccggacatcc
     4681 cggatcccgt cgatgcggtc gatgagaacc tcgcccgcga cccggatccc caggttcctc
     4741 atgaaggaga cgaccacctt cttcacgcag gtgaagttcg ccatccccgt cctccccgcc
     4801 accgagagga ggatgccctt cttctccccg ccgggataga aaaggttctt ttccgcatgg
     4861 tatgcctggc agcggtcgat gaagatcttg aggcccccgg ggacggtgtt cgtgtacacc
     4921 ggggtacaga tcacgaggac gggtgccgac cggagggccc ggatgagttc ggccatatcg
     4981 tcctcgaagg tgcaggaacc ggtgttgtag cactggtaac acccgatgca gggatggatg
     5041 acgagatcgt ccaggtacac cacccgtgcc ctcttcccga tccccgccgc ctcctctgcc
     5101 gcccagccgg cgaggatgct gcagttcccg tcgggccggg ggcttccctg gataatgagg
     5161 gcatcggtgg ttcccccggt aggggcagcc ttcaccgggg tttcgaggga ggcgaggaac
     5221 cccgcagggt ccctcaggag agccctctcc cactcggcgg ccttccggag gatggccttc
     5281 tccgcatcca ggggaatcgt cggggagtac tcgtaggagt tggtgcggaa ga
//
LOCUS       DATGDI010000121        14547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_12012, whole genome shotgun sequence.
ACCESSION   DATGDI010000121 DATGDI010000000
VERSION     DATGDI010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 14547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14547
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12012"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VKO45_04640"
     CDS             complement(<1..371)
                     /locus_tag="VKO45_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate synthase family protein"
                     /protein_id="HMA05196.1"
                     /translation="MGRAEEEKDVRELLLLAVRTLADSMVPSLLPGVGFRIVYALRGA
                     RRQEDVAGVEGGLVREGGKVRPAGGIAFGAGEGVAGMVLTAMHTDPEIRSAAAIRFSP
                     AIHGILGDLLLEVCEFDRAREP"
     gene            486..671
                     /locus_tag="VKO45_04645"
     CDS             486..671
                     /locus_tag="VKO45_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05197.1"
                     /translation="MNARMLDVATGITSLVVFIVALIVLSAVFPEGEKGLAYILAIAV
                     YIALMSGAGILIREKIA"
     gene            complement(652..1728)
                     /gene="priL"
                     /locus_tag="VKO45_04650"
     CDS             complement(652..1728)
                     /gene="priL"
                     /locus_tag="VKO45_04650"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF002591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase regulatory subunit PriL"
                     /protein_id="HMA05198.1"
                     /translation="MRVQLDRRDLAKYPFLKEAQEWVAGRTGTLDQFLATNYGKIAAR
                     NGKQRVLEAISGGEPGGNRPISSPEHEVFSYALARILVSCGKDRALIDRLTRFEAARA
                     FGFLQEEDPDKRSYVAESLGIDPDTEDLPVTEYVELVTQLREDRWRLVNRQVIRGRVQ
                     IGKDELEELVRERIRHLLHRQLPLPVPAQVCEVLSPLLADISATYQATVMRDFGEVEE
                     GAFPPCLQALVGAITAGTNLPHAGRFAITAFLHNIGMKPVQIMEIFARAPDFDPEKTR
                     YQVEHISGRGGTEYTAPSCATLRTTGFCVNRDSTCERVNHPLSYYRLKKRREKGRAKE
                     AEGEKGKAEEAGEKEKKPATRSSP"
     gene            complement(1728..2471)
                     /locus_tag="VKO45_04655"
     CDS             complement(1728..2471)
                     /locus_tag="VKO45_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase sliding clamp"
                     /protein_id="HMA05199.1"
                     /translation="MLKASIDADIFREAIEVVSALVTECRLHADANGIRTRAVDTANV
                     AMVSLDLGKEGFSAFSATKEEIGLDITKMKNILSMMSKGDLLSLQLGDNAKKLELSFG
                     SYRYSVTLLDVNTVRKDPNPPTIDLPGKVVISGEALSSAIKAASVVSDKIALGIDPKG
                     QKFYMVAEGDTDHIRLELGKDQLVSINPVEARSLFSLDYLKDLGKVMGRAKEVQVSLG
                     VDHPVKFSFTFADGKGSVDYLLAPRIEAD"
     gene            2590..3183
                     /locus_tag="VKO45_04660"
     CDS             2590..3183
                     /locus_tag="VKO45_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HMA05200.1"
                     /translation="MGSGWASDTVHRRAAKEGYRSRAAFKLLEIEDRFRVIRRGDSVI
                     DLGAAPGSWLQVVSSLTDGTVLGVDLQPIPPLPGVTTLVGDFTDPTVEERIRALVPGA
                     SVLLTDAAPHLTGNRSVDQARAVGIGGEALALACRVLKPGGNIVLKSFQGEDFNDLLD
                     AVRERFRSVHVFRPRVTRRGSRECYIVGKNFAGPESC"
     gene            3177..4052
                     /gene="moaA"
                     /locus_tag="VKO45_04665"
     CDS             3177..4052
                     /gene="moaA"
                     /locus_tag="VKO45_04665"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HMA05201.1"
                     /translation="MLIDTFGRPVTNLRVSVTQRCNLACIYCHAEGEKAPDAELPLGD
                     IREILRVAAGLGMRSVKFTGGEPLVRGDILEIVRAVPPGLESSLTTNGILLGKYAGGL
                     KEAGLSRVNVSLDSLDPACYRDITGHDRLSEVLAGIDAALEAGLTPIKLNMVVLKGIN
                     EDEVEAFLGYVRGNRDLILQLIELMELGSCVSHAEINRLEKDLESRSKIILTRRMHHR
                     KKYCVDGAEVEVVRPRHNTEFCAFCNRLRVTSDGKLKPCLLRSDNLIDIRGKRGGELE
                     ALFREAVRIREPYCR"
     gene            4172..4699
                     /locus_tag="VKO45_04670"
     CDS             4172..4699
                     /locus_tag="VKO45_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05202.1"
                     /translation="MLESNEYEQVLRHLYSKTLIMHNPSDFHPVLRFFFMDTLAHLDY
                     TIGTLAYNFMSIRNEMSREYLRWRVDEEEKGDRQKFRAFVNWLKVNHPDRFRSLPTVW
                     RIVYDDDNAAQYMSFRLVVDPDSNRPVPPGFFFDAAEEFFAQPFLMSLYTGSGLAKLF
                     EEFLLAPPAGGPGAG"
     gene            4789..6321
                     /locus_tag="VKO45_04675"
     CDS             4789..6321
                     /locus_tag="VKO45_04675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMA05203.1"
                     /translation="MAKAKEKKPGKGKGESPKEPGCDLEGPAPSAPVAGLAGILESVR
                     ASRYLQVLLVLTILGAFLRLFQLGAASLWLDEASTLTFARESVTQIWGSLSSATEFHP
                     PLFYWMEHAMLAFGDGEVVLRIIPAIFGILTIPVFYLLGAEILDRNTGLLAAALLTFS
                     PFQIYYSQEARDYTMMLFFFSLAFLFFLRLLRSWGRRDALLFGVFSALAFWAHFYAFV
                     PIVALLIFVLAVKAKEMARDPRSAIPLVISILVFSLLTLPLIYYAVQLFGKITGSAPT
                     FGTQGIQTISLTFMQISGFSEILAYAFVVLFVLGAASLFTRDRMRATLLVFAIVGPLL
                     LSIFLSYRMPMNPRYLIYLLAAFLPGIALLIRPACSLFRSRNLVYGALILALVINLPV
                     LVPYYTTQQKDDWRGASRELTGLAAPGDLVVAIPGYVHQPLDYYYGNETAGTLQFGLY
                     TVGDVQGLIASYPGRQVWFIVTGDIQATDTQGLLISWLGSHTRLTWRDSNGGILLLRY
                     TG"
     gene            6333..7052
                     /locus_tag="VKO45_04680"
     CDS             6333..7052
                     /locus_tag="VKO45_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HMA05204.1"
                     /translation="MDAKDSPLDLTVIIPTYNEGETIGTVIGAVEEVLDRAGIRGEIL
                     VVDDSSPDGTIPAVRALEAVIPNLRLAVRTSDPGLSQSVVEGFSLSRAPVLVVMDADL
                     SHPPELIPRFFEEIRNGADVVIGSRYMRGGAIEDWPAGRRVISWGATLLGRILFPGIT
                     DPVSGFFAVRREVVAGARLHPKGYKILMEVLGRGSWGKAKEIPFIFRDRKAGSSKLGP
                     GTILDYLRQVLGISFHALSGR"
     gene            complement(7026..8729)
                     /locus_tag="VKO45_04685"
     CDS             complement(7026..8729)
                     /locus_tag="VKO45_04685"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HMA05205.1"
                     /translation="MHRFFQSVRNKITLRHLQFLIAAIIVVLLVLSVNPGALSRFLFH
                     TSPLFLLLAMAFYLVNNLLMTYRLKRVLTFLGHKLRFRMTFSSHMAGMIFSDVTPARS
                     GYLYAAYDLSRKGIPLPRAMVSVTGTYVFDLIFKSLIAALGILYFYSSLFPLDARMYL
                     LLLFGFIAAGILLYAFITRAPQGVRGPLQRYRIFHLLFQYGDESRTLHRISPFILGIS
                     FLGWISRGFEWYCVAAALGIASFSILDGLFLNPLLTLLSLIPVTPAGIGIQEAGIVGL
                     FLLISIDRVAATSFALLTRAIEALIDAVGLASFYRSRVEHKDLHAFYGSIDGDIDERS
                     YNSDLFVQRYFQQRRTAAIMEHLEVGRGDVFLDIGCGSGVQMAMFARKDYALAIGIDL
                     NRNALSYARKRGLPNSEFIMADAGHLPFKAGVIRKILCAEVIEHMDRPDGLVQEMKRV
                     LGTGGEIVVTTPNENSPWGVYEFAWDVFGRGRNYADTHLKFYSPREIRELFQAFPEKE
                     VETLFFLSPFVALLNNNALVSAAERIDSLFERWSLGVLIIAHVKLGSPDRALSTREGV
                     E"
     gene            complement(8904..10121)
                     /locus_tag="VKO45_04690"
     CDS             complement(8904..10121)
                     /locus_tag="VKO45_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05206.1"
                     /translation="MKDRGKKRQRKEAEPARNRFGLPVISREKLLAMAVAVVGTRIVI
                     GLATTYLFNSFIDYYVLGIYLQAAMNVLQGMVPWANGTLFYYPPLALVPLLASFMAVP
                     VAGFLGFVVALWVLMAICDIVTTICVYYVGLKLYSERTAFIAAILNATAISVAYYSIT
                     RWEAYVVCLAMLAVLATVYERKTWGYIATIAGAFAKVWPALLYPFLWLYNARDGSILE
                     EGKKRAAWLLLAAGLAFGLMLWTGYNRFLEYAGMVYSNTVPYTVFEYLKLAGLAVEFS
                     TIATLFEVLLALVIIAALYGMYRRPQLTTMLKGILLVILVVTIFVQYRSPQYVAWLTP
                     FAALLIAGDRWGIFAFLGVQVMEYISFPLTFYSVWVNANYVTPWALPLFTVLFLMYGL
                     LAWRALKGKGCGE"
     gene            complement(10528..11883)
                     /locus_tag="VKO45_04695"
     CDS             complement(10528..11883)
                     /locus_tag="VKO45_04695"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HMA05207.1"
                     /translation="MTPIHSIILAGGIGTRLWPLSRRYYPKQFLQLSRRSLFQMTYGR
                     AGLLSGDDGITVVTNSIHQYLVRNQLEEMGCTLPEDRLLLESEGRNTLPAIAWAVGRI
                     GREDPDGLAAVFPSDHLVEESVVDEVRAASTLARENLVTFGVRPTRPHTGYGYIRPGG
                     DLGGGFRVSEFREKPDEATAARLIGEGCLWNSGIFLFSVRCFLSELARYQPGVGQAFR
                     TGEPGDEGIPSISMDHGLLEKSGRVAVVPLRARWDDLGDFGALYGNEPHDGAGNAGRA
                     EFIDAHGNFVHAGGKHVGVIGADDLVIVDTPDALLVCGREETGRVRELVERYDRRHDP
                     ITQFHLQVHRPWGSYTILEESGSYKIKRVSVSPGKRLSLQLHHRRSEHWIVVSGKAEI
                     QLGEEGFVLSPNESTFVRAGVRHRLGNPGKETLEVIEVQSGDYLGEDDIVRFDDEYGR
                     R"
     gene            12004..12699
                     /locus_tag="VKO45_04700"
     CDS             12004..12699
                     /locus_tag="VKO45_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05208.1"
                     /translation="MSSMRLAGVIAILLIILILTAGCGQRVPTRNTTTKNPWGNATVT
                     PKGGGGSSSGTTVVPTTTSPLIQATLMATPTTTPISGTSYRGPPLEANATANLTLFDE
                     QKMGPSYIGTAYNLNLTNTPLLIDLTMTVPNITRTVAEKDPTVVPSENDPNPTRTATK
                     TYPDPTAWFEVTVMDLKTKHIIARGGYGRTYDVSTHKQVWVRYPGSYHIEFTGARLTA
                     DVHFWMPNQTGAT"
     gene            12705..12842
                     /locus_tag="VKO45_04705"
     CDS             12705..12842
                     /locus_tag="VKO45_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05209.1"
                     /translation="MPACRDCFFFRPLTADEGECQMNGIVTADRDAERCPSRTFRPRA
                     G"
     gene            complement(12918..13505)
                     /locus_tag="VKO45_04710"
     CDS             complement(12918..13505)
                     /locus_tag="VKO45_04710"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMA05210.1"
                     /translation="MEAIRKVKGKMPGRRFMVDDPDRKQWQDPEEILSSVGLAAGMVF
                     VDLGCGEGYFTIPAARKVGPGGRVYAIDINPGAIGALRARAEEEGLGNIRSQVGEAET
                     TPVCEGCADLVFFGIDLHDFRDPVAVLKNAGRMLAPGGRIADLDWKDEPMPRGPPLSI
                     RFSVAKARSLIESAGFRVLSVRDTGPYHYLILAGL"
     gene            13620..13691
                     /locus_tag="VKO45_04715"
     tRNA            13620..13691
                     /locus_tag="VKO45_04715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13653..13655,aa:Arg,seq:gcg)
     gene            complement(13778..14470)
                     /locus_tag="VKO45_04720"
     CDS             complement(13778..14470)
                     /locus_tag="VKO45_04720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMA05211.1"
                     /translation="MMKGSPIHSLTYFTGTTDIRDQSNWQYLIHKYISNIDEQRILDV
                     GCASGFLIQELAKTSKFAVGIDIRPLDTAPGYPFIQADCHDLPFKDHSFDLVLSLGVL
                     EHVSDYELAITEMKRVLKPGGYLFLMMGPTPLWKYLDNPEHRKTITKHPEVTTVLRLL
                     QDGYVRKIWNENIEYRLFHMDTFQLFPTNPILAKLFRSCILRDFLSSILRFFEKNNME
                     QNICLIYHTIQG"
BASE COUNT         2773 a         4642 c         4340 g         2792 t
ORIGIN      
        1 ggttcccggg cccggtcgaa ctcgcagacc tcgaggagca gatccccgag gatgccgtgg
       61 atagccgggg agaaccggat cgcggcggca ctccggatct ccggatcggt gtgcatcgcg
      121 gtcagcacca tgccggccac cccttccccc gccccgaagg ctatgccgcc tgcaggccgg
      181 accttcccgc cttcccggac aaggcccccc tctaccccgg cgacgtcttc ctggcgcctt
      241 gctccccgga gggcatagac gatccgaaac cccactccgg gcaggaggga cgggaccatc
      301 gaatccgcga gggtcctgac ggcgaggagc aggagctccc tgacatcctt ctcctcctct
      361 gctcttccca ccgtccttac ctccggaatg taggtacgga tcatggaacc gggaaggtaa
      421 aaagagaacg atcgggggaa gggaatgggc aggcataagg ggaaggagga cggatagttt
      481 ctcccatgaa tgcccggatg ctggacgtgg cgaccggaat tacgagtctg gtcgtcttta
      541 tcgtcgcact catcgtcctc tccgccgttt tcccggaagg agaaaaaggg cttgcataca
      601 tcctcgccat tgcggtctac atcgcgctca tgagcggggc cgggatcctg atcagggaga
      661 agatcgcgta gccggttttt tttccttctc tcccgcttcc tccgcctttc ccttctcccc
      721 ctccgcctcc tttgcccttc ccttctcccg tctctttttc agccggtagt acgagagcgg
      781 atggttcacc cgttcgcagg tgctgtcccg gttcacgcag aagccggtcg tccggagggt
      841 tgcgcaggaa ggagcggtgt attccgtccc cccgcggccg gagatgtgct cgacctggta
      901 ccgtgtcttc tcgggatcga agtccggtgc ccgggcgaag atctccatga tctggaccgg
      961 cttcatgccg atgttgtgga gaaaggccgt gatggcgaac cggccggcat gggggaggtt
     1021 cgtcccggcg gtgatggccc ccacgagggc ctggaggcag gggggaaagg ccccctcctc
     1081 cacctccccg aagtcgcgca tgacggtcgc ctggtaggtg gcagagatat cggcgaggag
     1141 gggggagagg acctcgcaga cctgcgccgg cacgggaagg ggcagctgcc ggtggagaag
     1201 gtgccggatc cgttccctga cgagctcctc gagctcgtcc ttcccgatct gcacccttcc
     1261 ccggatgacc tgtcggttca cgagcctcca gcggtcctcc cgtagctgcg tcacgagctc
     1321 cacgtactcg gtcaccggga gatcctccgt atccgggtcg atgccgaggc tttcggcgac
     1381 ataggaccgc ttgtccgggt cctcctcctg gaggaacccg aaggcccggg ccgcctcgaa
     1441 ccgggtcagc cggtcgatga gggcccggtc cttcccgcag gacacgagga tccgggcgag
     1501 ggcgtaggag aagacctcat gctcggggga ggagatgggg cggttccccc ccggttcccc
     1561 tccggaaatt gcctcgagta ccctctgttt cccgttccgg gcggctatct ttccatagtt
     1621 cgtcgcgaga aactggtcga gggtgccggt ccggccggcg acccattcct gggcttcctt
     1681 caaaaacggg tatttggcga ggtcacgtct gtccagctgg acccgcatca atcagcctcg
     1741 atgcgggggg cgaggaggta gtccaccgaa cccttcccgt cggcgaaggt gaaggagaac
     1801 ttcaccgggt ggtccacccc gagggagacc tgcacctcct tcgccctccc catgaccttc
     1861 cccaggtcct tcaggtaatc gagggagaag agggaccggg cctccacggg gttgatggag
     1921 acgagctggt ccttcccgag ctcgagccgg atgtgatcgg tgtccccctc ggcgaccatg
     1981 tagaacttct gccccttcgg gtcgatgccg agggcgatct tgtcggagac caccgaggcg
     2041 gccttgatgg ccgaggagag ggcctcgccg gaaatgacca ccttcccggg gaggtcgatg
     2101 gtgggcgggt tcgggtcctt ccggacggta ttcacgtcga ggagggtgac ggagtaccgg
     2161 tagctcccga aggagagctc gagtttcttc gcattgtccc cgagctggag ggagaggagg
     2221 tcccccttgc tcatcatcga gaggatattc ttcatcttgg tgatgtcgag gccgatctcc
     2281 tccttcgtcg cagagaaggc ggaaaaccct tccttcccga ggtcgaggga aaccatcgcg
     2341 acatttgccg tgtccaccgc ccgggtccgg atcccgttcg catccgcgtg gagccggcac
     2401 tcggtcacga gggccgagac gacctcgatg gcctcccgga agatatctgc atcgatgctt
     2461 gccttgagca ttcctaaccc cttaacacac tctattaata actatataag catatttttt
     2521 aggtacattt gggttagcac gccgcgccgg gcctgctcga atgcccgggc cggcagggag
     2581 cattttcaga tgggttcagg atgggcgagt gataccgttc accggcgtgc cgcgaaggag
     2641 ggctaccggt cgagggccgc cttcaagctc ctggagatcg aagaccggtt ccgggtgatc
     2701 cggcgggggg actcggtcat cgacctgggg gcggcccccg gtagctggct ccaggtcgtc
     2761 tcgtccctca ccgacgggac tgtcctcggg gtagacctcc agccgatccc ccctctcccg
     2821 ggggtgacca ccctcgtggg ggacttcacc gatcccaccg tggaggaacg gatccgggcc
     2881 ctcgtgcccg gggcctcggt gctcctcacc gacgccgcgc cccacctcac cggcaaccgg
     2941 agtgtggacc aggctagggc cgtggggatc ggaggggagg cccttgccct cgcctgccgg
     3001 gtcctcaaac ccggcgggaa tatcgtcctc aaatccttcc agggggagga cttcaacgac
     3061 ctgctcgacg cggtcaggga acgcttccgc tcggtccacg tcttccggcc gagggttacg
     3121 cggaggggat cgagggagtg ctacatcgtc gggaagaact tcgcgggacc ggagtcatgc
     3181 tgatcgatac cttcggccgg ccggtgacga atctccgggt aagcgtcacc cagcgctgca
     3241 acctcgcgtg catctactgt catgccgagg gagagaaggc tccggatgcc gagctccctc
     3301 tcggagacat ccgggagatc ctccgggtgg ccgccggcct cggaatgcgc tccgtgaagt
     3361 tcaccggagg ggagcccctc gtccgcgggg atatcctgga gatcgtgcgg gcggttcccc
     3421 ccggtcttga gtcctccctg accacgaacg gcatcctgct cgggaagtac gccggcgggc
     3481 tgaaggaggc gggcctttcg agggtcaatg tctccctgga cagcctggac cccgcatgct
     3541 accgggacat caccggccac gaccgcctct cggaggtcct cgcggggatt gacgccgccc
     3601 tcgaggccgg cctgacgccc atcaagctga acatggtggt cctcaagggg atcaacgagg
     3661 acgaggtgga ggccttcctc gggtatgtgc gggggaaccg ggacctcatc ctccagctca
     3721 tcgagctcat ggaactcggg agctgcgtct cccacgcaga gatcaaccgg ctcgagaagg
     3781 acctcgagtc ccggtcgaag atcatcctta cccggcggat gcaccaccgg aagaagtact
     3841 gtgtcgacgg cgccgaggtg gaggtggtcc ggccccggca caacaccgag ttctgcgcct
     3901 tctgcaaccg gctccgggtg acctccgacg ggaaactgaa gccctgcctc ctccggtccg
     3961 acaatctcat cgatatccgg ggaaaacggg ggggggaact ggaggccctc ttccgcgagg
     4021 cggtgaggat ccgggagccg tactgtcggt gatctggacc ggcacacgct gccgctggtc
     4081 cggattgcag cagctcccgc cgattcggtt gtggcgggag aaaccggatg ggtgaaaagg
     4141 tttttaacgg cgggcccccc tttcagtctg tatgctcgaa tccaacgagt acgagcaggt
     4201 gctccggcac ctctacagta agaccctcat catgcacaat ccctcggatt tccacccggt
     4261 cctccgcttt ttcttcatgg acaccctcgc ccacctcgat tataccatcg ggactctcgc
     4321 gtacaacttc atgtccatca ggaacgagat gtcccgggaa tacctccggt ggcgggtgga
     4381 cgaggaggaa aagggggaca ggcagaagtt ccgggccttc gtcaactggc tgaaggtgaa
     4441 ccacccggat cgcttccggt cgctgccgac ggtctggagg atcgtgtacg acgatgacaa
     4501 cgccgcccag tacatgagct tccggctggt cgtggacccg gacagcaacc gtcccgtgcc
     4561 accgggattc ttcttcgatg ccgccgagga gttcttcgcc cagcccttcc tgatgagcct
     4621 gtacaccggg tccggccttg ccaagctctt cgaggagttc ctcctcgcac cccctgcagg
     4681 gggcccggga gccggttgat ccgacttcaa gcatttctcc cctcggggcc ggcccctgcc
     4741 gggacgatcc tcttttaacc ccctgtctcc cacctactat ttgatctcat ggcgaaggca
     4801 aaggagaaaa aaccggggaa ggggaagggg gaatccccga aagaacccgg ctgtgacctt
     4861 gagggcccgg caccgtccgc ccccgttgca ggcctggccg gaatccttga atcggtccgg
     4921 gcaagccggt atctccaggt cctcctcgtc ctcacgatcc tcggggcatt ccttcgcctc
     4981 ttccagctcg gggcagcctc cctctggctc gacgaggcct ccaccctcac cttcgcccgc
     5041 gagtcggtga cccagatctg gggctcgctc tcctctgcca ccgagtttca cccgcccctc
     5101 ttctactgga tggagcatgc catgctcgcc ttcggggacg gcgaggtggt cctccgcatc
     5161 atccccgcca tcttcgggat cctcaccatc cccgtcttct acctcctcgg agccgagatc
     5221 ctcgaccgga acaccgggct cctcgccgca gccctcctca ccttctcccc cttccagatc
     5281 tactattccc aggaggcccg tgactatacc atgatgctct tcttcttctc cctcgccttc
     5341 ctcttcttcc tccggctcct cagatcctgg gggaggaggg acgccctcct cttcggggtc
     5401 ttttcagccc tcgccttctg ggctcacttc tacgccttcg tgccgatcgt cgccctcctc
     5461 atcttcgtcc tcgccgtgaa ggcgaaggag atggcacgcg atccccgttc ggcgatcccc
     5521 ctcgtcatct ccatcctcgt cttctccctc cttaccctcc ccctcatcta ttacgccgtc
     5581 cagctcttcg gcaagataac gggctcagca cctaccttcg gcacccaggg gatccagacc
     5641 atctccctga ccttcatgca gatatccggg ttctcggaga tccttgccta tgccttcgtg
     5701 gtccttttcg tcctcggggc cgcctccctc ttcacgaggg accgcatgag ggccaccctc
     5761 ctcgtcttcg ccatcgtcgg ccctctcctc ctctccatct tcctctccta ccggatgccc
     5821 atgaacccga ggtacctcat ctacctcctc gcggccttcc tcccgggtat cgccctcctg
     5881 atcaggcctg catgctccct cttcaggagc aggaacctcg tgtacggtgc cctcatcctt
     5941 gccctggtca tcaacctgcc ggtcctcgtt ccctactaca ccacgcagca gaaggacgac
     6001 tggaggggtg cctcccggga actcaccggc ctcgcggcac ccggggacct ggtggtcgcc
     6061 atccccggct acgtgcacca gcccctcgat tattactacg ggaacgagac cgcggggacc
     6121 ctgcagttcg gcctgtacac cgtcggggat gtacagggac tcatcgcatc gtatcccggc
     6181 cgccaggtct ggttcatcgt cacgggggat atccaggcaa cagacaccca gggcctcctc
     6241 atctcctggc tgggttcgca cacccggctt acctggaggg actcaaacgg cgggatcctc
     6301 ctcctcaggt atacaggcta gaggctgacc tcatggatgc gaaggattcc ccgcttgatc
     6361 ttaccgttat catcccgacc tacaatgaag gggagaccat cgggacggtc atcggggcgg
     6421 tagaggaggt cctcgaccgg gccggcatac gcggggagat cctcgtggtc gacgattctt
     6481 cccccgacgg caccatcccc gcggtacggg cccttgaggc ggtcatcccg aacctgcgcc
     6541 tcgcggtccg gacttcagat cccggtctct cccagtctgt ggtggagggg ttctctcttt
     6601 cacgtgcccc cgttcttgtg gtgatggatg ccgatctctc tcatccgccc gagctcattc
     6661 cccggttctt tgaggagatc cgtaacggtg ccgacgtggt catcggttcg cgctacatgc
     6721 ggggcggggc catcgaggac tggccagcgg gcaggcgggt catctcgtgg ggagcgaccc
     6781 tcctcgggcg gatcctcttt cccgggatca ccgacccggt gagcggcttt ttcgccgtga
     6841 ggagggaggt ggtcgcaggg gcacgccttc acccgaaggg ctacaagatc ctcatggagg
     6901 ttctcgggag gggatcgtgg ggaaaggcga aggagatccc ctttatcttc cgggacagga
     6961 aggcgggatc gagcaagctg ggcccgggca cgattctcga ctacctccgt caggtcctcg
     7021 gcatctcatt ccacgccctc tcgggtcgat agggcccggt cgggactccc cagcttcacg
     7081 tgggcgatga tgagaacccc gaggctccac ctctcgaaga gggaatcgat cctttccgcc
     7141 gctgagacga gggcattatt gttgaggaga gcgacgaagg gggacaggaa gaagagggtc
     7201 tccacctcct tttcgggaaa tgcctggaag agctcccgga tctcccgggg ggagtaaaac
     7261 ttcagatggg tatctgcgta attcctcccc ctcccgaaga cgtcccaggc gaactcgtag
     7321 actccccagg gtgagttctc gttgggagtg gtgaccacga tctctccccc cgtccccagc
     7381 acgcgtttca tctcctgcac gaggccgtct ggccggtcca tgtgctcgat aacctctgcg
     7441 cagaggatct tccggatgac accggccttg aaggggaggt gcccggcatc ggccatgatg
     7501 aattccgaat tcgggaggcc cctcttcctc gcataggaga gggcattccg gttcagatcg
     7561 atcccgatgg cgagagcgta gtccttccgg gcaaacatgg ccatctggac accgcttccg
     7621 caccctatat cgaggaagac gtcccccctc ccgacctcca ggtgctccat gatggctgcc
     7681 gtcctcctct gctggaagta gcgctgcacg aagaggtccg agttgtacga cctctcgtcg
     7741 atatccccgt cgatggaacc atagaaggcg tggagatcct tgtgctcgac ccggctccgg
     7801 tagaaactgg ccaggccgac cgcgtcgatg agggcctcga tggcccgggt gagaagggcg
     7861 aaggaggtgg ccgcgacccg gtcgatgctg atgagcagga agagcccgac gatgcccgcc
     7921 tcctggatcc cgatgcccgc cggcgtcacg gggatgaggg agaggagggt aaggagcggg
     7981 ttcaggaaga ggccgtccag gatggagaag gatgcaatcc ccagggcggc ggcgacacag
     8041 taccattcga acccgcggga gatccagccg aggaaggata tgccgaggat aaagggcgag
     8101 atccggtgga gggtccggct ctcatccccg tactggaaga gcaggtgaaa tatccggtat
     8161 ctctggaggg gccctctcac cccctgagga gcccgggtga tgaaggcgta gaggaggatg
     8221 ccggccgcga tgaacccgaa gaggaggagg aggtacatcc gggcatcgag gggaaagagg
     8281 ctcgaataaa aatagaggat ccccagggcc gcgatgaggg acttgaatat caggtcaaag
     8341 acgtacgtgc ccgtcaccga aaccatcgcc ctgggaagcg ggatcccctt cctgctcaga
     8401 tcatatgccg catacaggta cccgctccgt gccggggtca cgtccgagaa gatcatcccc
     8461 gccatgtggg aggagaaggt catgcggaat ctcagcttat gcccgaggaa cgtgaggacc
     8521 cttttcaagc ggtatgtcat gaggaggtta ttcacgaggt agaaggccat ggcgaggagg
     8581 aggaacaggg ggctcgtatg gaagaggaac cgggagagtg cccccgggtt gaccgagaga
     8641 acgagaagga cgacgatgat cgccgcgatg agaaactgaa gatgcctcag ggtgatttta
     8701 ttccgtacgg actggaagaa tcggtgcata ctcccctggc cctctgcaat ccccatccgg
     8761 atgacctgta ggcgttcttc ggctttaccc ggtgctccct gtcgctctcg aacggaaaac
     8821 cggctccctg gcgaggaatc cccgggcggg agccttgccc attaccctgt ggcgcgggat
     8881 cttcgtcagg cggaaggagg gattcattct ccacatccct tccccttcag cgcccgccat
     8941 gcgagcagcc cgtacatcag gaaaaggacc gtgaaaagcg gcagggccca cggggtgacg
     9001 taattggcat tcacccacac cgagtaaaag gtgaggggga acgagatgta ctccatgacc
     9061 tgcaccccca agaaggcaaa gattccccac ctgtctccgg cgatgagaag cgcggcgaag
     9121 ggggtcagcc aggcaacgta ctgcggggaa cgatactgca cgaagatcgt tacgacgagg
     9181 atcacgagga gtattccctt cagcatcgtg gtgagctggg ggcgcctgta catcccgtag
     9241 agcgccgcga tgatgacaag ggcgagcaat acctcgaata gggtggcaat cgtgctgaac
     9301 tcgacggcga gcccggcaag cttcagatat tcaaaaaccg tgtatgggac ggtgtttgag
     9361 tacaccatgc cagcatactc gaggaacctg ttatacccgg tccagagcat cagcccgaag
     9421 gccaggccgg ctgcgaggag gagccatgcg gctcttttct tcccttcctc gaggatcgag
     9481 ccgtcccgtg cgttgtagag ccataggaac ggatagagga gcgcaggcca gaccttggcg
     9541 aatgcccccg caatggtcgc aatgtacccc catgtcttcc tttcgtacac ggttgccagc
     9601 accgcgagca tcgcaaggca caccacgtac gcctcccagc gggtgatgga atagtacgcg
     9661 accgagatcg ccgtcgcatt caggattgcc gcgatgaagg ccgtcctctc actgtatagc
     9721 ttcaggccga cgtagtagac acagatggtg gtcacgatgt cgcagatcgc catcagcacc
     9781 cacaacgcca ccacgaagcc gaggaaaccc gccaccggga cggccataaa cgaagccagc
     9841 aggggaacca gggccagggg tgggtaatag aacagcgtcc cgttcgccca gggtaccatc
     9901 ccctggagga cgttcatggc tgcctggaga tagatcccga ggacgtagta gtcgatgaag
     9961 gaattgaaca ggtaggtcgt ggcgagcccg atgacgatcc gcgtgcccac aacggccaca
    10021 gccatcgcga ggagcttttc acgactgatg acggggagcc cgaacctgtt ccgggcaggt
    10081 tctgcctcct ttctctgtct cttcttccct ctgtccttca tacaacctcc tcggtggtga
    10141 tccggagagt cttctcctca ggcgggatca aaacccgcgt aagcagacct tctgtgtctc
    10201 cctcgtatcc attgacccgg tctcctgtca tgccgtgatt caccgcgacg ggcaggacgc
    10261 catacatgcc atccgggagg gggtggagga aggtggagaa gggacggcag actgctccgg
    10321 taagacgggg gagggccggg gagcctgcag aatgcaggga aggcgatgga aacatggaaa
    10381 tttcctgatg ctccccgata atcctgtgaa ccccccagat cccgctcatt ttgccccgta
    10441 tatcatgtct ccccggacgc tataagaaaa ttgtcatgcc ttccccgtac cggggatccg
    10501 acgcgggcgc cccccttcct cccggggtca cctcctcccg tactcgtcgt cgaaccggac
    10561 gatgtcgtcc tcgcccaggt agtccccgct ctgcacctcg atgacctcga gggtttcctt
    10621 ccccgggttc ccgaggcggt gccggacccc tgcacggacg aaggtgctct cgttcgggga
    10681 gaggacgaac ccctcctccc cgagctggat ctccgccttc cccgaaacga cgatccagtg
    10741 ctcgctccgc cggtgatgga gctggaggga gagacgttta ccgggactca cgctcacgcg
    10801 cttgatcttg taggacccgg actcctcgag gatggtgtag gagccccagg gacggtggac
    10861 ctggaggtgg aactgggtga tggggtcatg gcgccggtcg taccgttcca cgagctcccg
    10921 cacccttccc gtctcctccc gcccgcagac gaggagggca tcgggggtat cgacgatgac
    10981 gaggtcgtcc gccccgatga ccccgacatg cttgcccccg gcatggacga aatttccatg
    11041 ggcatcgatg aactccgccc ggcctgcatt ccccgccccg tcgtgaggct cgtttccgta
    11101 cagggcgccg aagtccccga gatcgtccca cctcgcccgg aggggcacga ccgcgaccct
    11161 tcctgacttc tcgagcaggc catggtccat cgagatggac gggatgcctt cgtccccggg
    11221 ttcccccgtc cggaaggcct gccccacccc gggctggtac cgcgcgagct cggagaggaa
    11281 gcagcgcacc gagaagagaa agatcccgct gttccagagg cagccctctc cgatgaggcg
    11341 tgccgccgtc gcctcatcgg gcttttccct gaactccgag acccggaatc ccccgccgag
    11401 atccccgccc ggccggatgt acccgtaccc ggtgtgggga cgggtcggcc tcacaccgaa
    11461 ggtgaccagg ttttcccggg cgagggtgga ggcggcccgg acctcgtcca ccaccgattc
    11521 ctccacgagg tggtcgctcg ggaagacggc cgcgaggccg tcgggatcct ctctccctat
    11581 ccgtcccacc gcccacgcga tggcgggaag ggtattcctg ccctccgatt cgaggagcag
    11641 ccgatcctcc gggagggtgc accccatctc ctccagctgg ttcctgacca ggtactggtg
    11701 gatggagttc gtgaccacgg tgatgccatc atcccccgaa aggagtcccg cccgcccgta
    11761 ggtcatctgg aagagggacc tccgggagag ctggaggaac tgtttcgggt aatatcgacg
    11821 ggagagaggc cagagccggg tcccgatgcc cccggcaagg ataatggaat ggatcggggt
    11881 catcgagaaa cgtgggattc ggtatcatat tagatattct gttgtaaaat ccccctaatc
    11941 cggcccgaga tggagttcat actggataaa ggaataaata tatgattcat caaatattca
    12001 ctgatgagtt ccatgcggct ggccggggta atcgcaattc ttctcatcat cctgatcctc
    12061 accgcgggat gcggccagag ggtccccacg cggaacacta cgaccaagaa cccgtggggg
    12121 aatgccaccg tcacccccaa agggggcggc ggctcctcct cgggaacgac agtggtcccg
    12181 acgaccacga gccccctcat acaggcgacc ctcatggcga cgccgacgac gacccccatt
    12241 tcggggacct cctaccgcgg acccccgctc gaagccaatg ccacggcaaa cttgaccctc
    12301 ttcgacgagc aaaagatggg tccctcgtac atcgggaccg cgtacaacct caacctcacg
    12361 aacacgcccc tcctcatcga ccttaccatg accgtgccca atatcacgag aaccgttgcc
    12421 gagaaggatc ccacggtagt gccatcagaa aatgacccca atcctacccg gacggccacc
    12481 aagacctacc ccgatcccac cgcatggttc gaggtcacgg taatggacct gaagacaaaa
    12541 catatcatcg cgagaggagg gtacggccgg acgtatgacg tgtccaccca taagcaggtc
    12601 tgggtccgct atccaggtag ttaccatatc gagttcacgg gggcccggct gaccgcggac
    12661 gtccatttct ggatgcccaa ccagacggga gccacctgag ggatatgccg gcctgccgtg
    12721 actgtttctt cttccggccc ctcaccgcgg acgaggggga atgccagatg aacggcatcg
    12781 tcaccgccga ccgtgatgca gagagatgcc cgtcgcgcac cttccggccc agggccggat
    12841 gaggaaccat tcaccttctc ttcgttatcg ctgttattga tccgaacccg ggaacggacg
    12901 attcccatgg tacatcccta aaggcctgcc aggatgaggt aatggtaggg tccggtatcc
    12961 ctgaccgaga ggaccctgaa cccggcggac tcgatcaggg accgcgcctt cgcaacggag
    13021 aatcggatag agaggggcgg gccccggggc atcggttcgt ccttccagtc gaggtcggcg
    13081 atccgccccc cgggcgcaag cattctcccc gcatttttga ggaccgccac gggatccctg
    13141 aaatcatgga ggtcgatccc gaagaacacc agatccgcac atccctcgca gaccggtgtc
    13201 gtttcagcct cgcccacctg agaccggatg tttcccaggc cctcctcctc tgcccgggca
    13261 cggagcgccc cgatggcccc gggattgata tcgatggcat agacccttcc cccggggccc
    13321 accttcctcg ccgcgggaat cgtgaagtac ccctccccgc atccaaggtc gacgaagacc
    13381 atccccgccg cgaggccgac ggaggagagg atctcctcag gatcctgcca ctgtttcctg
    13441 tccggatcgt ccaccatgaa ccgccggccg ggcattttgc ctttcacctt ccggatcgct
    13501 tccatagtca ggtcgcgagg aaccgataac aacgtatcgg aggggatatc ggtgcagccg
    13561 ggacacatgg gcaaccgttt taaggctaca ggacaatccc tttgagtaat ttccctcgcg
    13621 tcccggtggg gtagtggaca tcctagaagc ctgcggagct tttgacccgg gttcaagtcc
    13681 cggccggggc gtaccacttt tcacgtcaat acctcttccc gctccatcga gtctggttga
    13741 tgagcggttg gcgcatccca ccgtaaggta agatgcctta cccctgaata gtatgataga
    13801 tcagacaaat gttctgttcc atattattct tctcgaaaaa tcgcaggatt gacgaaagaa
    13861 aatctctgag gatacaactc ctgaaaagct tcgccaaaat cggattcgtt ggaaatagct
    13921 gaaaggtgtc catgtgaaac agacggtatt ctatattttc attccagatt ttccggacat
    13981 acccatcctg taacaagcgt agcaccgtag taacttccgg gtgtttcgtt atggttttcc
    14041 gatgctcagg attatcaagg tatttccaca atggggtagg tcccatcatc aggaaaaggt
    14101 aaccccctgg cttcaggact cgtttcattt ctgtgatcgc cagctcgtaa tcagatacgt
    14161 gttcaagaac cccgagcgat agaaccagat caaaggagtg gtctttgaac ggtaagtcat
    14221 gacaatctgc ctgaatgaat ggatagccag gtgcggtgtc caaaggccgt atgtcaatac
    14281 caaccgcaaa cttgctggtt ttggccaatt cctgaattaa aaaacccgag gcgcagccaa
    14341 catcaagaat cctttgttca tcgatattcg agatatactt atgaatcaga tactgccagt
    14401 ttgactgatc cctgatgtcg gtcgtgccgg taaagtaggt aagggaatga atgggagatc
    14461 ccttcatcat ggcagctcac caccaattgg attgtctgac ttcacatact ctccatcctt
    14521 atgcagcttt ccaaaagcct tcaggtt
//
LOCUS       DATGDI010000122         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_244835, whole genome shotgun sequence.
ACCESSION   DATGDI010000122 DATGDI010000000
VERSION     DATGDI010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244835"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VKO45_04725"
     CDS             complement(<1..270)
                     /locus_tag="VKO45_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05212.1"
                     /translation="MADSSAYGLWTLVILYSVVFIGFAYAFTRPRTSRDWKSLGAFSA
                     FMVALFTEMYGFPLTIYLLSGWLGSRVPGLDLLSHNAGHLWYTVLG"
     gene            complement(399..989)
                     /locus_tag="VKO45_04730"
     CDS             complement(399..989)
                     /locus_tag="VKO45_04730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMA05213.1"
                     /translation="MHIQSESVADFNRWSRTYETSWMQRSLFDRVHEGVLDLVVRDSA
                     PGVILDAGCGTGRLLRKARIRYPGARLIGVDPAEGMIEKARQMLPDAAFSVGSAEALP
                     LPDASVDLAVSTISYHHWKDQEQGVREIRRVLRPGGQFLLADMVLPAFLVKVLRHGHI
                     RTAGEVKAIFGRAGLEVLSQHRLLLGLILVTVGIRR"
     gene            complement(1078..1221)
                     /locus_tag="VKO45_04735"
     CDS             complement(1078..1221)
                     /locus_tag="VKO45_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HMA05214.1"
                     /translation="MAIDPVCKMTVDEKTAKITSDYKGKKYYFCNPGCKKKFDADPAK
                     YAK"
     gene            1407..1480
                     /locus_tag="VKO45_04740"
     tRNA            1407..1480
                     /locus_tag="VKO45_04740"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1441..1443,aa:Cys,seq:gca)
     gene            1504..2763
                     /gene="cysS"
                     /locus_tag="VKO45_04745"
     CDS             1504..2763
                     /gene="cysS"
                     /locus_tag="VKO45_04745"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HMA05215.1"
                     /translation="MDLYSTLSGKVEALVTLHPDRVTMFVCGPTVYDYSHLGHARTYV
                     VFDVLAKYLRWTGKEVFYLQNITDVDDKIIRRAGEEGITQSQLARKYEAEYFRDMRAL
                     GIDGVSLYAHATTHIPEILSQVERLLARGVAYITEAGVYFNVDTFEGNGELSGQDRAK
                     RVSRVVDETKHNPQDFALWKFGEFGEYTWDSPWGRGRPGWHIEDTAISERYLGQQYDI
                     HGGGLDLIFPHHEAEIAQMESLDGRRPMVRYWVHTGFLTVQGEKMSKSLGNFITIRDA
                     LKTWSRDVLRYFILLSHYRSPLQATEEGILNASRGLDHIRAVAKKDAGPNQEGRKAFT
                     DAMESDLNTPAALAAIQGLASAGDLGALREFGEILGINFLRETSAPLSILRDVRRELR
                     SRKQFEVSDLIRARMVDAGLTITDEPL"
     gene            complement(2897..>3428)
                     /locus_tag="VKO45_04750"
     CDS             complement(2897..>3428)
                     /locus_tag="VKO45_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HMA05216.1"
                     /translation="GGAVMLSDREIYRRILFNQNAVGAILSPFDSFLVARGIKTLALR
                     MERHEKNALAVGRYLEEHRKTAAVYYPGLPSHPQHDLARRQMTGFSGMLSFEIRGGEA
                     EARRFLGALRVFALAESLGGVESLIEHPATMTHSTIPKPEREKVGITDTLVRVSVGIE
                     NVEDLIGDLGQALDRV"
BASE COUNT          664 a         1069 c         1066 g          629 t
ORIGIN      
        1 gccgaggacc gtgtaccaga gatgccccgc gttgtgcgag agcaggtcaa ggccaggcac
       61 ccggctgccc agccagccgg agaggaggta gatggtcagc gggaagccgt acatctcggt
      121 gaagagggcg accatgaagg ccgagaatgc cccgagggac ttccagtccc gggaggtccg
      181 cgggcgcgtg aaggcatagg cgaagccgat gaagacgacc gagtagagga tgacaagggt
      241 ccagagcccg taggcagatg aatcagccat gcttttcaac tccgtttcta tccggggacc
      301 cttcctccgg aggtcccctg tcttccttac cggtaccggg gctccaggta tgaacgtgac
      361 cctgtcggca agacctgccg cgatttcccc gtcctggttt aacgcctgat cccgaccgtg
      421 acgagaatga gcccgagcag cagccggtgc tgcgacagga cttccaggcc tgcccgcccg
      481 aagatcgcct tcacttcccc ggcagtccgg atgtggccgt ggcgtaggac cttcaccaga
      541 aacgccggga gaaccatgtc ggcgagaagg aactgccctc cggggcgcag gacacgccgg
      601 atctcccgca cgccctgttc ctggtccttc cagtggtggt atgagatggt actcaccgca
      661 agatccaccg aggcatcagg aaggggtaag gcttctgccg aacccacgga gaaggcggca
      721 tccggtaaca tctggcgcgc cttttcaatc atcccctctg ccggatccac cccgatcagc
      781 cgggctccgg ggtaccggat cctcgccttt cgcagcaacc ttccggtccc gcatccggca
      841 tccaggatga ccccgggggc ggagtcacgg acgacgagat cgaggacacc ttcgtgcacg
      901 cgatcgaaga gagaccgctg catccacgat gtctcgtagg tccgtgacca gcggttgaaa
      961 tccgcaacac tttcagattg tatgtgcatg gatcgtttcg ccctgtctcc agcttcacag
     1021 tctttgggta aactcccgga tgggatttta aaaataatat ctccttcgtg cctgctctta
     1081 cttcgcgtac ttcgccgggt cggcgtcgaa cttcttcttg cacccggggt tgcagaaata
     1141 gtacttcttc cccttgtaat cgctcgtgat cttcgcggtc ttttcatcca ccgtcatctt
     1201 acagactggg tcgattgcca tgtgtgcacc tcagtgctgc gatcttgttt ccctctcaat
     1261 attttaatgg atcgggtacc ggcaatctct gcctcaccgg ccaaattttg cctcttcgag
     1321 aatcctctta tagacgccat gccgatacta tttcgccccc cccgggaaca ccgtggaacg
     1381 tccatgaagg ggggggccgg gaaagagccg gtgtggcaga acggatcaat gccttcgccc
     1441 gcaaagcgaa tgtctggggg ttcgactccc tccgccggcg tgagaaaggg agagatcagc
     1501 accatggacc tgtatagcac gctctcgggg aaggtggagg ccctggtcac ccttcacccc
     1561 gaccgggtca cgatgttcgt ctgcggcccg acggtatacg actactccca cctcggccac
     1621 gcgaggacgt acgtcgtttt cgacgtgctc gccaagtacc tccggtggac ggggaaggag
     1681 gtattctacc tccagaacat caccgatgtg gatgacaaga tcatcaggcg ggcaggggag
     1741 gaggggataa cccagagcca gctggcgcgg aaatacgagg ccgagtactt cagggatatg
     1801 agggccctcg gcatcgacgg ggtcagtctc tatgcccatg ccaccaccca catccctgag
     1861 atcctcagcc aggtagaacg cctgctggcc cggggcgtgg catacatcac agaggccggt
     1921 gtctacttca acgtggacac cttcgaggga aacggggaac tgtccggcca ggacagggca
     1981 aagcgggtca gccgggtggt ggacgaaacg aagcacaacc cccaggactt cgccctctgg
     2041 aagttcggcg aattcgggga gtacacctgg gactccccgt ggggcagggg ccggcccggc
     2101 tggcatatcg aggacacggc gatatccgag cggtacctcg ggcagcagta cgacatccat
     2161 ggcggggggc tggacctgat cttcccccat cacgaggcag agatagccca gatggagtcc
     2221 ctggatggca ggcgcccgat ggtgaggtac tgggtgcaca ccggcttcct gacggtccag
     2281 ggagagaaga tgtccaagtc cctcgggaac ttcattacca tccgggacgc attgaagacg
     2341 tggagcaggg atgttctccg atatttcatc ctcctctccc actatcggtc ccccctccag
     2401 gccaccgagg aagggatcct gaacgcctcg cgcgggctcg atcatatccg ggcggtggca
     2461 aagaaagacg caggcccgaa tcaggagggg aggaaggcct tcaccgacgc catggagagc
     2521 gatctcaata cccctgctgc cctcgcggcg atccaggggc ttgcctccgc cggggatctt
     2581 ggagccctga gggagttcgg ggagatcctc ggcatcaact tcctccggga gacgagcgcc
     2641 cccctaagca tcctccggga cgtgaggagg gagctccgtt cccggaagca gttcgaagtc
     2701 tcggacctga ttcgggctcg aatggtcgac gcgggcctaa ctatcaccga cgaaccgctc
     2761 tgatgttccc tgccatggaa gggaaaggta cgcctgggcc aggtgagaca gtggtgtggg
     2821 gtctccccga tggtgcgggg ccggctctct cggaagggtt tttaccggcc cgcacgacgg
     2881 ggccgtgacg ccctctttac acccggtcga gcgcctgccc gaggtccccg ataaggtcct
     2941 ccacgttctc gatacccacg gagacccgga cgagggtgtc cgtgatcccc accttttccc
     3001 gctcgggctt cgggatggtg gagtgggtca tcgtcgccgg gtgctcgatg agggactcga
     3061 ccccgcccag gctctcggca agggcgaaca cccggagcgc cccgaggaac ctccgtgcct
     3121 cggcctcccc gccccggatc tcgaaggaga gcatccccga gaagccggtc atctgccgac
     3181 gggcgaggtc atgctgcgga tgcgacggca gcccgggata gtagacggca gcggtcttcc
     3241 ggtgctcctc taggtatctg cccacggcga gggcattctt ctcgtgccgt tccatccgga
     3301 gggcgagggt cttgatcccc cgcgcgacca ggaagctgtc gaagggggaa aggatcgccc
     3361 ccacggcgtt ctggttgaac aggatcctcc ggtagatctc cctgtcagag agcatgaccg
     3421 cgccgccc
//
LOCUS       DATGDI010000123        18680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_41233, whole genome shotgun sequence.
ACCESSION   DATGDI010000123 DATGDI010000000
VERSION     DATGDI010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 18680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18680
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41233"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VKO45_04755"
     CDS             <1..396
                     /locus_tag="VKO45_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05217.1"
                     /translation="ARTELIHISLDRIAHPIAPKRVKMPTGAVRRIRVSTISPIPGAR
                     FPIGVGILIGTPATIPRSIAVVTAERIRRKSGVRAKMYRERNPLGSEPVSRTIFAFPD
                     PGVRIEPSMRKNGRMPINSFNLTGILPGF"
     gene            556..750
                     /locus_tag="VKO45_04760"
     CDS             556..750
                     /locus_tag="VKO45_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17e"
                     /protein_id="HMA05218.1"
                     /translation="MGIKPSYIKRLGEELIEGRPERFGRDFKENKQGVSENSIIGSKK
                     VRNRVAGYITRKHNTGKKSS"
     gene            929..1834
                     /gene="dapA"
                     /locus_tag="VKO45_04765"
     CDS             929..1834
                     /gene="dapA"
                     /locus_tag="VKO45_04765"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HMA05219.1"
                     /translation="MFEGVFPAIITPFTRGNRPATDDRSDLDLEGLRANVAWLVEQGV
                     HGIVPCGSTGESATMSHKEHEKVIEETVDVVNGRVPVLAGTGSNNTEEALLLTKAAKN
                     LGADGVLVISPYYNKPNRSGLFKHYTKIANLDIPVVMYNVPGRTGQDMNNFQPPLVPE
                     LAKHPNIVGIKEASGKLDKISQIIEATRDQNFSVISGDDNLTLPILELGGVGVISVAA
                     NVEPRLMVEMYEAFKKGNLARAREIHSKLGPLFKDLFIDTNPIPVKKAVGLRGMAAGP
                     VRLPLDDLDAEKTAILKKTLDSYRA"
     gene            1847..2614
                     /gene="dapB"
                     /locus_tag="VKO45_04770"
     CDS             1847..2614
                     /gene="dapB"
                     /locus_tag="VKO45_04770"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HMA05220.1"
                     /translation="MIKVVVCGALGRMGGTVAKLVHEASDLELAGGVDVRAGTAFGAE
                     VVASNQIEAFLEKKKPDVLVDFTVASAAVENVKAAARHGVAAVVGTTGFTPKQRDEMK
                     RAVEASVPAVITSNFSVGVNIFWFLVREAAKKLGDYDIEVIEAHHRNKKDAPSGTAKT
                     ILEILDAEVGTRPKLYGREGMMERGKEIGVHVIRGGDIVGEHTVLFAGNQEAIELTHR
                     AQDRSVFAMGALRAIRWVGGKKPGVYTMADVLNLHPK"
     gene            2771..2944
                     /locus_tag="VKO45_04775"
     CDS             2771..2944
                     /locus_tag="VKO45_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA05221.1"
                     /translation="MPAHVESDLCVGCETCVDECPAEAITMEGGVAKVDKKKCTECKT
                     CMDVCPSAAIRLE"
     gene            3149..4165
                     /gene="asd"
                     /locus_tag="VKO45_04780"
     CDS             3149..4165
                     /gene="asd"
                     /locus_tag="VKO45_04780"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HMA05222.1"
                     /translation="MIKVGVLGATGAVGQRFVQLLANHPWFDLVTLTASERSAGKKYG
                     DVVNWKLDAPFPEKVRNIIVSPTSVKGARGAELVFSALPAEIAGSVEDDFSRAGMAVC
                     SNARSHRMEPDIPLVIPEVNPDHLALIDVQRDAGREGFVVTNPNCSTIMLCVALEPLR
                     SMKFTDVRVATMQAISGAGFSGVAAMDIYDNVVPYIGQEEEKMETETLKIMGTLTGGL
                     VRDAPFRVSASCHRVPVIDGHTLAIWVDVKEGVEKVKQAFRNYRPPFSGLPTQPEKSI
                     ELFEEDNRPQPRLDRMRGGGMTVSVGRVRPGLRFIALGHNTIRGAAGASVMNAELIRT
                     KNYL"
     gene            4179..4454
                     /gene="albA"
                     /locus_tag="VKO45_04785"
     CDS             4179..4454
                     /gene="albA"
                     /locus_tag="VKO45_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HMA05223.1"
                     /translation="MLKDNTVFVGNKPVMNYVLAVVTQFNNGAEEVAIKARGKAISRA
                     VDTAEIALNRFLDNVAKKEILTSTEVIDTDSGKTNVSSIEIILAQKT"
     gene            4559..5839
                     /locus_tag="VKO45_04790"
     CDS             4559..5839
                     /locus_tag="VKO45_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMA05224.1"
                     /translation="MSPPVPFRDRVPELARREYPDLEALYRELHAHPELSGLEKETAA
                     RMGAELGRSGLVVHRGIGGHGVVGVLENGPGPAVMIRCDMDALPVTEMTGLPYASTVM
                     TSVETRGVKVGVMHACGHDLHMAILAGAVRLLAATRDTWSGTLLAVVQPAEETGAGAR
                     AMVRDGIFTRFPRPVSALALHVDPNIPAGKIGYRPGIISAGSTSLDVTVRGIGGHAAH
                     PHQSIDPVVLAARVVLALQTIVSREIDPREMAVLTVGAIHGGTKHNAIPDEVVLRVNI
                     RYNSDAVRDRMLASIDRIVKGIALSAGVPEELAPVIRRPGETTPPMVNDPLLTEQVRE
                     ILASVLGAENVLLIPQLTGSEDFSVFSNGSPAPAICYLRLGAADPRDLEESRKTGRDL
                     PYLHSSRFAPPPDPTIRVGAIALAAAVNGLLAPR"
     gene            complement(5879..6166)
                     /locus_tag="VKO45_04795"
     CDS             complement(5879..6166)
                     /locus_tag="VKO45_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HMA05225.1"
                     /translation="MTAEEEKLTGEILSWIKKYAKEKKLAINTDDRQFAAVVRGLVRN
                     RLKHGERYCPCRIRSGDPEKDRLIICPCSYHEKEIESEGHCHCNLFFKPGS"
     gene            complement(6214..6912)
                     /gene="purQ"
                     /locus_tag="VKO45_04800"
     CDS             complement(6214..6912)
                     /gene="purQ"
                     /locus_tag="VKO45_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HMA05226.1"
                     /translation="MRFAVVRFGGSNCDLDAVHVLRHVCGVETDLLWYKEGLRRKYDA
                     VVIPGGFSYGDYLRAGAIAARTPVMEEILRHAKEGGLILGICNGAQIGAESRLVPGTF
                     TLNAYPKFLCRPVHLRVENSGTPFTSLYREGEVIRLPIAHKEGRYMADPETLAALNRE
                     KRVVFRFSDADGRVTEAVNHNGSAENITGVLGRGGNFLAMMPHPERASEAVLGSDDGK
                     RIFLSMIRHIEENT"
     gene            complement(6909..7154)
                     /gene="purS"
                     /locus_tag="VKO45_04805"
     CDS             complement(6909..7154)
                     /gene="purS"
                     /locus_tag="VKO45_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HMA05227.1"
                     /translation="MNFDVEVRISLKPGILDPEARAIQHALSDLGFPTTRLSTARIFS
                     ISLEAGDRAEAGEMARQMCERLLANPVIHRYEVEVRE"
     gene            complement(7151..7912)
                     /gene="purC"
                     /locus_tag="VKO45_04810"
     CDS             complement(7151..7912)
                     /gene="purC"
                     /locus_tag="VKO45_04810"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HMA05228.1"
                     /translation="MKKGALLYRGKAKSVYLTDTPDELSVEFRDDITAFDGGKMSVLP
                     DKGVYNAAVSAFFFEYLEIHGVRTQYIHMLDRRTMLVRRLSMIPLEVIVRNVAAGSLV
                     KRYPFKEGEVLKPTIIMLDYKDDSRHDPMVNDDIIFALGLVSREELLTVRKMALKVND
                     LLRTYLAGLAITLVDFKLEFGRWKDQILVGDEISMDSMRLWDERTRVSLDKDVFRFDK
                     GDVMATYAAVAERITPGALHRFEAEKAVFAGDEQG"
     gene            complement(7909..8892)
                     /gene="cofG"
                     /locus_tag="VKO45_04815"
     CDS             complement(7909..8892)
                     /gene="cofG"
                     /locus_tag="VKO45_04815"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HMA05229.1"
                     /translation="MMARVITFSRNVFLPLTTVCRNRCDYCGFRTPVREGCILSPAGV
                     DRILVHGAATGCTEALFTFGERPETEPGFSAMLSALGYNTILDYCYGMCLRAIATGLL
                     PHTNAGLLTYGDMERLREVNASMGLMLETTAEVPAHRNSPGKRPEERIRMMEDAGRLR
                     IPFTTGILVGIGETLGDREESLMVIRDLQKRFGHIQEVIVQNFCPKEGTPMARAPGVS
                     TDEMCLTLRLARDILPAEVAVQIPPNIADAAALIPCGVDDLGGISPVTPDYINPEHSW
                     PAVDDLWKVAGEAELRERLCIYPGFIEKGWYPPAIEGLIRRLEQQIRGEKR"
     gene            8979..9611
                     /gene="cofC"
                     /locus_tag="VKO45_04820"
     CDS             8979..9611
                     /gene="cofC"
                     /locus_tag="VKO45_04820"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HMA05230.1"
                     /translation="MPIRAIIPFKPKNPKTRLSKIMSLAERETFAEMMLGDVIEAVVD
                     GGCTPTILSTAPYCLTGIETIVHPGGLNEALNELLPKAEGPTLIVMSDLPLITPDNIT
                     EITTTKARAAMVPGRGGGTNAIFLPNPAGFRADFYGASFLKHLEIAGELGLSVEIIDS
                     FRLHDDIDEVEDLVEVLTLGSGGTRRYLERLGFALATEDGRVGVKRNPHE"
     gene            complement(9540..10349)
                     /gene="cofE"
                     /locus_tag="VKO45_04825"
     CDS             complement(9540..10349)
                     /gene="cofE"
                     /locus_tag="VKO45_04825"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HMA05231.1"
                     /translation="MKTGFSVFGVKTPLIRAGDDLPSIILEAAGKSPAKGVRDGDILV
                     IAESPVATAEGRAVSLDRVTPSDRARELAGRYGMDPRLAEVVLGESDAVVGGVAGFLL
                     TMKQGTLLPNAGVDLSNTPPGTALPLPRDPDGSSARIRRALEERTGAKVAVIIADSRT
                     HAMRLGCSSVAIGCAGIRAVIDERGRTDLYGHILEVTQRAVADNIASAAVLVMGEADE
                     SVPCAIVRGLGLPIVDEVGVPSIAAEECLFMGVALHADPSVLGGKGEPEPL"
     gene            complement(10346..11155)
                     /gene="folP"
                     /locus_tag="VKO45_04830"
     CDS             complement(10346..11155)
                     /gene="folP"
                     /locus_tag="VKO45_04830"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HMA05232.1"
                     /translation="MQACVIRGLSIGDGSPVRLMGVINCSPESFYRGSYVPPGKAGEK
                     ALTMVADGADLVDVGARATGPGSPPLSPEVERERMVAALSALEGTGVTVSVDTREPEV
                     LAACLRYDIHAVNDIGGLSDPRFALRVRDSGLPAILMASREVPGDAIGLQATLDALQL
                     VVTRCMEYDIDDFILDPGIGKWVPERTARDDWELVRHFAEFRSFGRPLLAAVSRKTFI
                     GELLRREPGERLSGSLAVALLLALGGASVVRTHDVRETRDVLGVLAEVKGP"
     gene            complement(11140..11979)
                     /locus_tag="VKO45_04835"
     CDS             complement(11140..11979)
                     /locus_tag="VKO45_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HMA05233.1"
                     /translation="MILDGRATADKRLEGLKEQIRASGITPRLATVMVGKDPGSQLYV
                     RMKHRTCEQVGIGSVSVELLEGAASGEVEGEIRALGENPSIDGILVQLPLPRHLDERR
                     IIAAIPPEKDVDGFTPFSLGRLFSGNPAFVPCTPLGVMTLLSEYGIPVEGADAVVVGR
                     SLEVGRPMAALLLNAGSTVTICHSKTRDLAGKTGSADILVSAVGKPGFITGDMVKKGV
                     VAVDVGITMVGGKPVGDLDFPSVEPVARAITPVPGGVGPMTIAMLMENTLAAARERRC
                     RPA"
     gene            complement(11981..13219)
                     /gene="glyA"
                     /locus_tag="VKO45_04840"
     CDS             complement(11981..13219)
                     /gene="glyA"
                     /locus_tag="VKO45_04840"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HMA05234.1"
                     /translation="MSELARTDPEIADVIEKERLRQLNGLELIASENIVSRAVLEAQG
                     SIMTNKYAEGYPGKRYYGGCGYYDVAENLARDRLKQLFGAEHANVQPHSGTQANMAAY
                     FAAMKPGGTLMSMSLSQGGHLSHGSPVNFSGQLFRIVPYGVDLETETLDYGVIESIAR
                     KEKPNVIVCGASAYPRTIDFKAFREIADAVDAKCVADIAHIAGLCATGVHPTSVNVAH
                     FTTTTTHKTLRGPRGGAIMCGKEFAQAVDKAVFPGMQGGPLMHTIAAKAVCFKEALRP
                     QFTDYNRQIVKNCRVLAQTLMDGGLRLVSGGTDNHLLLIDLSDKGITGLEAENALGCA
                     GITVNKNTIPRETRSPFVTSGLRIGTPAVTSRGMKEPEMRFIGAKILHVIRDVKDEKA
                     LVQVKEEVIAMASRFPIYPE"
     gene            13570..13995
                     /locus_tag="VKO45_04845"
     CDS             13570..13995
                     /locus_tag="VKO45_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05235.1"
                     /translation="MRVPQAGSDRVSELENKVREMEALIKGLTNELLDLKSIVERLNS
                     RFEEKRPPLLTRPQGRVSVSPRGQPGEGAPASEGELARIMQSDGTMKEERRKDSDYIV
                     ASAKTAQALRSKGKGEPSKGKGSNALIYAVEEEDEKSKQ"
     gene            14041..17481
                     /gene="smc"
                     /locus_tag="VKO45_04850"
     CDS             14041..17481
                     /gene="smc"
                     /locus_tag="VKO45_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HMA05236.1"
                     /translation="MHITEIEIDNFKSFGRKTTIPFFEHFTVISGPNGSGKSNIIDAI
                     LFSLALSSSRTLRAEKLTDLINLNTGKNTAEVAIRFSDETVVRRRIRRTPHGYYSYNY
                     LNNRLCKQTDITEFLGKVGIKPEGYNVVMQGDITRIMEMSDTERRKILDEIAGVAEFD
                     SKKEAALRELEVVRERIDREELMLHELDTRLQELEAERDRAVEYRALLDTIHRFEVCR
                     SAAELREREKELLALRELSAEQGKALEKAKGAAADVAGAIGKLNGELGELDLQINEKS
                     GPEYLSLVSGLEAEKGKITLAEQTIGRISKEKEGNLEEINRIYLDQKRAEAKVTEGTQ
                     AIRTLSIDRANLAMEAGSLRGKFEVLDRDVKSRTSNVESSRDRLFSLMEEVEKKKTAR
                     AEVIHKKDLLIEKSRGRTSERERLQKEVEAAEAEMAEKDRQVAECGTSVADREAGKQG
                     LEREIAAAEGRLFALRAEAERLETEGRAKERELARLEAQQQAGGEGTERGLEAVRGLD
                     GVHGTIRELGKADPEYATALSVAAGGRIRNIVVDNDRVAADAITLLKEQKLGRFTFLP
                     LKNLKNVNLPPLEARPGVIDYAVNLIHYDPVYAPAFRIVFGRTVVVDTLDRARKLLGK
                     YRMVTLEGELLEESGAMTGGSLKKVKGFGIAAEDDIARLRAEIQGLAAETAEVRAGIQ
                     REEARSTDLRARRTTLDEEMARFRILAEEYGKRRDALTEERAATAAALAKMDEETGGG
                     AQELAACEQELEEMTREISRVTGEVEELKRALDDDALPGLVQEMERLRTEADEVEKRL
                     RNKDAEISGAQQERSYFTKRVEELSAEKERVAGKNREIDATVASSKEEIERAKGAIVS
                     LEERLSVFSKELSGLREARDKLTEKIRTAERKRADREAGIERIALQISALEEKEKAIA
                     PALEALKVQAAGIETELSLQEIEEGLSEAKKGVEGMGAVNMLAIEEYERTTARVKERK
                     ERKEILSRERASILERIEQYEKMKHSAFSTAFSAIDGNFREIFARLTRGSGHLVLENE
                     EDPFAGGLTFAVQPRDKKVHLLSALSGGEKSLTTLAFIFSIQKYMPAPFYALDEIDMF
                     LDGYNVEQIANMIKELSANAQFVIVSLRKPMIDSADRIVGVTLREDKSSLVTGVKASG
                     "
     gene            17577..18347
                     /locus_tag="VKO45_04855"
     CDS             17577..18347
                     /locus_tag="VKO45_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HMA05237.1"
                     /translation="MDEEPVEILVGLAERGEIDPWNIDILEVTDRFLNELDRLRQLDL
                     RVSGRTLFYAATLLRLKSEFLVEPDATGEEDEFGPEEEPPEGFDIHEWLGPIERLERE
                     IRRRIERKNLRTRPVTLYELIRMLRTAEREEHRRRRTRTVTPEFIPETADVVSVAHDE
                     GYQETAGDVWKRWVELAASREEIRLGELCQTLDRKIADVYIPLLFLNLDGKVEISQET
                     FFEEVFIRRVPEKAHDREPVKNAENGKRKTRKREQAAE"
     gene            complement(18418..>18680)
                     /locus_tag="VKO45_04860"
     CDS             complement(18418..>18680)
                     /locus_tag="VKO45_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05238.1"
                     /translation="LYPRSVPHDLNKMDVSGYIACGRRENGQKSGSINAELPGNVKLP
                     AGGSSRERWGAQSDVPIRATELEHGEERRFRSATLPKEAELK"
BASE COUNT         3487 a         6029 c         6145 g         3019 t
ORIGIN      
        1 gccaggaccg agctgatcca catttccctt gaccgtatcg cccacccgat cgccccgaaa
       61 agggtaaaga tgccgaccgg ggcggtgagg aggatcagag tctccaccat atctcccata
      121 cccggggcca gatttcccat cggcgtgggg atactcatcg gaactccggc cacgattccc
      181 cggagcatcg ccgtcgtaac tgcggaaaga atcaggagga agagcggggt gagggccaag
      241 atgtaccggg agaggaaccc tctgggatcg gaacccgttt ccaggacgat cttcgcgttc
      301 ccagatcccg gagtccggat cgagccttcc atgcggaaaa atgggcggat gcccataaat
      361 agtttcaacc tgaccgggat acttccgggg ttttgaacag cgcgagcggt gacgggtgga
      421 ccgggcccgg ggaacggcat ccccccctgt tttcccgcag gaaggccggg aaaaaagggt
      481 gcagaccatg gcaatccgct gttcgcaaat attcttaagg tcccgcgacg catccatgat
      541 gcacaaggga gatccatggg aatcaaacca tcctacatca agagactggg cgaggagctc
      601 atcgaggggc gacccgaacg gttcggccgg gacttcaagg agaacaagca gggcgtatca
      661 gagaactcga tcatcgggag caagaaggtc cggaaccggg tcgccgggta catcacccgg
      721 aagcacaaca ccgggaagaa gtcctcgtaa ggccggtctc ctcttttcct attttaccgg
      781 ctgttctccc ggtcccccat gcatccttca ccttcttgag ggtttccccg agagccgggg
      841 ttctttggaa cagaggggcg gatattcgaa agcgataaga ataaggtgcg cgctggtaga
      901 agctgcaggc aggtgaggct acagcgctat gttcgagggt gtctttccgg ctatcatcac
      961 gcccttcacc cgggggaatc gcccggcgac ggatgatcgc tcggatctcg atctcgaagg
     1021 attgcgtgcg aacgtggcat ggctcgtgga gcagggcgtg catggcatcg tgccctgcgg
     1081 ctcgacgggg gagtccgcga ccatgagcca caaggagcac gagaaggtca tcgaggagac
     1141 cgtggacgtg gtgaacggga gggtaccggt ccttgccggg acgggctcca acaataccga
     1201 ggaggccctc cttctcacca aggcagcaaa gaacctcgga gcggacgggg tcctcgtcat
     1261 ctccccgtac tacaacaagc cgaacaggtc cggccttttc aagcactaca cgaagatcgc
     1321 gaatctcgac atcccggtcg tcatgtacaa cgttcccggc cggacggggc aggatatgaa
     1381 caattttcaa cccccgttgg tacccgaatt ggcaaaacat cccaatatcg tcgggatcaa
     1441 agaggcaagc ggaaagcttg acaaaatttc acagatcatt gaggcgaccc gggaccagaa
     1501 cttcagtgtc atctccggcg atgacaacct gaccctcccc atcctcgagc tcgggggggt
     1561 cggggttatc tcggttgcgg cgaacgtgga gccgaggctc atggtggaga tgtacgaggc
     1621 cttcaagaag gggaatcttg cccgcgcccg ggagattcac tcaaaactcg ggccactctt
     1681 caaggatctt ttcatcgata cgaatccaat cccggtaaag aaggcggtgg ggttacgggg
     1741 tatggcggcc ggcccggtcc ggctccccct ggatgacctg gacgccgaaa agactgcgat
     1801 cctgaagaag acccttgaca gctacagggc ttgaggagga accggcatga tcaaggtggt
     1861 cgtctgcggc gccctgggcc ggatgggcgg cacggtggca aaactcgtcc acgaggcatc
     1921 tgacctcgag ctcgcgggcg gggtggatgt ccgggcggga accgccttcg gggcggaggt
     1981 ggtggcgtcg aaccagatcg aggccttcct cgagaagaag aagcccgacg tgctcgtgga
     2041 cttcaccgtg gcctctgcgg cggtcgagaa cgtgaaggcc gcggcccggc acggcgttgc
     2101 ggcggtcgtg ggaaccaccg gcttcacccc caagcagcgg gacgagatga agcgggccgt
     2161 ggaggcgtcg gtccccgcgg tcatcacgag caacttctcg gtcggggtga acatcttctg
     2221 gttcctcgtc cgggaggccg cgaagaagct cggggactat gacatcgagg tcatcgaggc
     2281 ccaccaccgg aacaagaagg acgccccgag cgggacggcc aagaccatcc tcgagatcct
     2341 ggacgccgag gtcgggaccc ggccgaagct ctacggacgg gagggaatga tggagcgggg
     2401 aaaggagatc ggggtccatg tcatccgggg cggggacatc gtcggggagc atacggtgct
     2461 cttcgccggg aaccaggagg ccatcgagct cacccaccgg gcccaggacc gctcggtctt
     2521 tgcgatgggt gccctccggg ccatccgctg ggtgggaggg aagaaaccgg gggtctacac
     2581 gatggccgac gtgctgaacc tccacccgaa gtgagccctg gccctgaaaa atcgcacctt
     2641 cggtgctcaa atcacccccc tgtttggggg gctgaacatc gaaaggctca cgcctttctc
     2701 tttctcccct cctgaaggga gggtcgaata tcgaaaccta ttagtccttt tttcccgaac
     2761 atatacgaga atgccagcac acgtggaatc cgatctctgc gtggggtgcg agacctgcgt
     2821 cgacgaatgt ccggccgagg ccatcaccat ggaggggggc gtcgcgaagg tggacaagaa
     2881 gaagtgcacc gagtgcaaga cctgcatgga cgtctgcccc tcggccgcca tcaggctgga
     2941 gtgagccgca ggcccttcca ccaccttacc ggccgattca tttcttcttc cgtccctccg
     3001 gaaccgtgaa aaatccatat cctccgccca ccaggcctcg tttgaaccgg ttccggttct
     3061 ccgcgccttc ccggatccaa gcccggattt cccggcgggc ggacaaagca aattatttat
     3121 tgagtgaccg ctcctaacac tctctgccat gatcaaggtc ggagtgctcg gagcgaccgg
     3181 ggccgtcgga cagcggttcg tccagctgct cgccaaccac ccctggttcg acctcgtcac
     3241 cctcaccgcc tcggagagga gtgccgggaa gaagtacggg gacgtggtga actggaagct
     3301 cgatgccccc ttcccggaga aggtgaggaa catcatcgtg tcccccacga gcgtcaaggg
     3361 ggcgaggggg gcggaactcg tcttctccgc ccttccggcc gagatcgcag gctcggtcga
     3421 ggacgacttc tcccgggcgg ggatggcggt ctgcagcaat gcccggtccc accggatgga
     3481 gccggacatc cccctcgtca tccccgaggt gaaccccgac cacctggccc tcatcgacgt
     3541 ccagagggac gcggggagag agggtttcgt cgtcacgaac ccgaactgct ccaccatcat
     3601 gctctgcgtg gccctcgagc ccctccggtc catgaaattc accgatgtcc gggtcgcgac
     3661 catgcaggcc atctccgggg ccggcttctc cggggtcgcg gccatggaca tctacgacaa
     3721 cgtcgtcccc tacatcgggc aggaggagga gaagatggag accgagaccc tgaagatcat
     3781 gggcaccctc acgggaggcc tcgtgaggga cgcacccttc cgggtgagtg ccagctgcca
     3841 ccgggtgccg gtcatcgacg ggcacaccct tgccatctgg gtggacgtga aggaaggggt
     3901 cgagaaggtg aagcaggcct tccggaatta ccggcccccc ttctccggtc tgccaacgca
     3961 gccggagaag tccatcgagc tcttcgagga ggacaaccgc ccgcagcccc ggctcgaccg
     4021 gatgcggggc ggggggatga cggtctccgt cgggagagtc cggccgggcc tgcgcttcat
     4081 cgccctcggc cacaacacca tccggggggc tgcaggcgcc tccgtgatga acgcagagct
     4141 gatccgcacc aagaattacc tctgaggggg ggagccccat gctgaaggac aacacagtgt
     4201 tcgtcggaaa caagccagtc atgaactacg tgctcgcagt ggtgacccag ttcaacaacg
     4261 gtgcagaaga ggtggcgatc aaggcgaggg ggaaggccat ctcccgggcg gtggacaccg
     4321 ccgagatcgc cctcaaccgt ttcctcgaca acgtcgcgaa gaaggagatc ctcacctcca
     4381 ccgaggtgat cgataccgac agcgggaaga cgaacgtctc cagtatcgag atcatcctcg
     4441 cccagaagac gtgaagggcg gagacctgac ttcccgtttc ctgcccggag tcggcaggga
     4501 gagacccggc cacgactccc gcgggtgatc cgcggggaaa tgcacgagga gaagaaagat
     4561 gagccctccg gttcccttcc gtgatcgcgt tcccgagctt gcccgccggg aataccctga
     4621 tctcgaggcc ctctaccgcg agctccacgc ccaccccgag ctctccggcc tagagaagga
     4681 gaccgccgcg aggatggggg ccgagctcgg ccggtcgggg cttgtggtcc accggggaat
     4741 cggcgggcac ggggtcgtgg gggtccttga aaacggaccc ggcccggccg tgatgatccg
     4801 gtgcgacatg gacgccctcc ccgtaaccga gatgaccggc ctcccctacg cgagcaccgt
     4861 catgacctcc gtagagaccc ggggggtgaa ggtcggggtg atgcatgcct gcggccatga
     4921 cctgcacatg gcgatcctgg cgggcgcggt ccggctcctc gcggcgacga gggacacctg
     4981 gtcgggcacc ctcctcgctg ttgtccagcc ggccgaggag accggggcgg gcgcccgggc
     5041 catggtccgg gacggcatct ttacccggtt cccccgtccg gtttcggccc tcgccctcca
     5101 cgttgacccg aatatcccgg ccgggaagat cgggtaccgg cccggcatca tctcggcggg
     5161 cagcacctcc ctcgacgtga ccgtccgggg catcgggggc catgccgcgc acccccacca
     5221 gtccatcgac ccggtggtcc tcgccgcccg ggtggtcctt gccctccaga ccatcgtctc
     5281 ccgggagatc gacccgaggg agatggcggt tctcaccgtc ggggccatcc acgggggcac
     5341 gaagcacaac gccatccccg acgaggtggt gctgcgggtg aatatccggt acaacagcga
     5401 tgcggtccgg gaccggatgc tcgcctccat cgaccggatc gtcaagggaa ttgccctctc
     5461 cgcgggtgtc ccggaggagc ttgcaccggt catccgacgg cccggggaga cgacgccccc
     5521 gatggtgaac gatcccctcc tcaccgagca ggtccgggag atcctcgcct cggtcctcgg
     5581 cgcggagaac gtcctcctca tcccccagct caccgggagc gaggacttca gcgtcttttc
     5641 gaacgggagc ccggcgcccg ccatctgcta cctccgcctc ggcgcggcag acccgcgcga
     5701 tctggaggag agcagaaaga cggggaggga tctcccctac ctccacagca gccggttcgc
     5761 cccgccaccg gaccccacga tccgggtcgg cgccatcgcc ctcgcagcgg cggtgaacgg
     5821 ccttctcgcg ccccggtgat cgagggatgc ggaggtggtc cgtccggcct ggaacttttc
     5881 agcttccggg cttgaagaaa aggttgcagt ggcagtgccc ctcgctctcg atctcctttt
     5941 cgtggtagct gcacgggcag atgatgagcc ggtccttctc cggatctcct gagcggatcc
     6001 ggcaggggca gtaccgctca ccgtgcttca gccggttcct gaccagcccc cggacaacgg
     6061 cggcgaactg ccggtcgtcc gtattgatgg ccagtttctt ctccttcgcg tacttcttga
     6121 tccaggagag gatttcgccg gtgagcttct cctcctcggc ggtcatctcc ttcttctcct
     6181 cccccttccc cttcgtccct gccatggaag acctcacgtg ttctcctcga tgtgcctgat
     6241 catggagagg aagatccgct tcccgtcgtc agacccgagg accgcctcgg aggcccgctc
     6301 ggggtggggc atcatggcga ggaagttccc tcccctgccg aggacccccg tgatgttctc
     6361 cgccgatcca ttgtggttga ccgcctccgt gacccgcccg tcggcatctg agaaccggaa
     6421 cacgaccctc ttctcccggt tcagggccgc gagggtctcc gggtctgcca tgtaccgccc
     6481 ctccttgtgg gcgatgggga gccggatcac ctccccctcg cggtagaggg aggtgaaggg
     6541 ggtcccgctg ttttcgaccc gcaggtggac cgggcggcag aggaacttcg ggtacgcgtt
     6601 cagggtgaag gtgcccggga cgagccggct ctcggccccg atctgggcgc cgttgcagat
     6661 cccgaggatc aggcccccct ccttcgcgtg ccggaggatc tcctccatga caggcgtcct
     6721 tgccgcgatg gccccggccc ggaggtaatc cccgtacgag aacccgccgg ggatcaccac
     6781 ggcatcgtat ttccgccgga ggccctcctt gtaccagagg aggtcggtct ccaccccgca
     6841 gacgtgacgg agcacatgga cggcgtccag gtcgcagttg gaacccccga accggacgac
     6901 cgcgaacctc attccctgac ctccacctca tagcggtgga tgacggggtt cgcgaggagc
     6961 cgttcgcaca tctgcctggc catctccccg gcctcggccc ggtcccccgc ctccagcgag
     7021 atggagaaga tccgggccgt ggagagccgg gtcgtgggaa agccgaggtc cgagagggcg
     7081 tgctggatgg cccgggcctc gggatcgagg atcccgggct ttaaggagat cctcacctcg
     7141 acatcgaagt tcatccctgc tcatctcccg cgaagaccgc cttttctgcc tcgaaccggt
     7201 gaagggcacc gggggtgatc ctctcggcca ccgccgcata ggtcgccatc acgtccccct
     7261 tgtcaaaacg gaacacgtcc ttgtccaggg acacccgggt cctctcgtcc cagagccgca
     7321 tggagtccat ggagatctcg tcccccacga ggatctggtc cttccacctg ccgaactcga
     7381 gcttgaagtc gacgagggtg atggcgaggc ccgcgaggta ggtccggagg aggtcgttca
     7441 ccttgagggc catcttcctc acggtgagga gctcctcccg gctgacgagg cccagggcga
     7501 agatgatgtc gtcgttcacc atgggatcgt gccggctgtc gtccttgtag tcgagcatga
     7561 tgatcgtggg cttcagcacc tccccctcct tgaaggggta ccgcttcacg agggatcccg
     7621 ccgcgacgtt ccggacgatg acctcgaggg ggatcatgga gagccgccgg acgagcatgg
     7681 tccgccggtc gagcatgtgg atgtactggg tcctgacccc gtggatctcc aggtactcga
     7741 agaaaaaagc cgagaccgcg gcgttgtaca ctcccttgtc cgggaggacg gacatcttcc
     7801 ctccgtcgaa ggccgtgatg tcgtcccgga actccacgga cagctcgtcc ggggtgtcgg
     7861 tcaggtacac cgacttcgcc tttcccctgt agagaagcgc gcccttcttc accgtttctc
     7921 ccccctgatc tgttgctcca gccgtctgat aagtccttcg atggcgggag gataccatcc
     7981 cttctcaatg aaccccgggt agatgcagag gcgttccctg agctccgcct cccccgccac
     8041 cttccaaagg tcatcgaccg ccggccagga gtgctccggg ttgatgtagt cgggagttac
     8101 cggcgagatg cccccgagat cgtccacgcc gcaggggatg agggcagcgg catcggcgat
     8161 gttcggcggg atctggaccg cgacctctgc cgggaggatg tcccgggcaa gccggagggt
     8221 gaggcacatc tcatcggtgg acactcccgg cgcccgggcc atcggggtcc cctccttcgg
     8281 gcagaagttc tggacgatga cctcctggat gtggccaaac ctcttttgga ggtcccggat
     8341 gaccatgagg gactcctccc ggtctcccag ggtctcgccg atccccacca ggatgcccgt
     8401 ggtgaagggg atgcggagcc ttcccgcgtc ctccatcatc cggatccgct cctcgggccg
     8461 cttccccggc gagttccggt gagcggggac ctccgccgtc gtctcgagca tgaggcccat
     8521 cgaggcgttc acctcccgga gacgctccat atccccgtag gtaaggagac ccgcgttcgt
     8581 gtggggaagg aggcccgtcg caatggcccg gaggcacatc ccgtagcagt aatcgaggat
     8641 cgtattatag cccagggccg aaagcatggc agagaacccg ggttcggtct ccggccgctc
     8701 gccgaaggta aagagggcct cggtgcatcc ggttgcggcc ccgtggacaa gtatccggtc
     8761 caccccggcg ggagaaagga tgcatccttc ccgcacagga gtccggaacc cacagtagtc
     8821 gcaccggttc ctgcagaccg tggtgagggg gaggaagacg tttctcgaga aggtaatgac
     8881 ccgggccatg atgtactcct tcccctcccg gctcatgaag ctggccgggc gggatcatcg
     8941 aattgttttt ctcatccccg gaaccacccc tgtatcgcat gcccatccgc gccatcatcc
     9001 ccttcaagcc gaagaacccg aagacccggc tctcgaagat catgagcctc gcagagcggg
     9061 agaccttcgc ggagatgatg ctcggtgacg tgatcgaagc ggtggtcgac ggggggtgta
     9121 ccccgacgat cctctccacc gccccctact gcctgaccgg catcgagacc atcgtccacc
     9181 caggaggcct caacgaggct ctgaatgagc tcctcccgaa ggcagaaggt ccgaccctca
     9241 tcgtcatgtc cgatctccct cttatcaccc ccgacaacat caccgagatc acgaccacga
     9301 aggcccgggc tgccatggtg ccgggccggg gagggggaac gaacgccatc ttcctcccca
     9361 accccgcggg cttccgggcc gacttttacg gggcgagctt cctgaagcac ctcgagatcg
     9421 ccggggagct cggcctctcg gtggagatca tcgactcctt ccggctccac gacgacatcg
     9481 acgaagtgga agatctcgtg gaggtcctca ccctggggag cggcgggacc cggaggtacc
     9541 tagagcggct cgggttcgcc cttgccaccg aggacggacg ggtcggcgtg aagcgcaacc
     9601 cccatgaata ggcactcctc ggcggcgatg gaggggaccc ccacctcgtc cacgatggga
     9661 aggccgagcc ccctcacgat ggcgcagggg acggactcgt ccgcctcccc cataacgagg
     9721 accgccgccg aggcgatgtt gtcggccacg gcccgctggg tgacctcgag gatgtggccg
     9781 tagaggtcgg tcctcccccg ctcgtcgatc accgcccgga tcccggcgca gccgatggcc
     9841 accgaggagc agccgaggcg catggcatgg gtccggctgt cggcgatgat caccgcgacc
     9901 ttcgctcccg tccgttcctc cagggcgcga cggatccggg ctgagctccc gtcgggatcc
     9961 ctcgggaggg gcagcgcggt ccccggcggg gtgttggaca ggtccacccc ggcgttcggg
    10021 aggagggttc cctgcttcat ggtgaggagg aagcccgcga cgccgcccac gacggcgtcg
    10081 ctctccccga ggaccacctc ggcgagccgg gggtccatcc cgtaccggcc cgcgagctcc
    10141 cgggcccggt ccgagggtgt cacccggtcg agggatacgg cccgaccctc ggcggtggcg
    10201 accggcgact ccgcgatgac aaggatgtcc ccgtcccgca cccccttcgc cgggctcttc
    10261 ccggccgcct ccaggatgat ggacgggaga tcgtcaccgg cccggatgag gggcgtcttc
    10321 accccgaaga ccgagaaacc cgttttcacg ggcccttcac ctcggcgagg accccgagga
    10381 catccctcgt ctcccgcacg tcgtgggtgc gtaccactga cgcccccccc agggcgagga
    10441 ggagggcaac cgcgagggac ccggagagcc gttcgccggg ttctcttcgc aggagctccc
    10501 cgataaaggt cttccgggag accgccgcga ggagcggccg gccaaaggag cggaactccg
    10561 cgaagtgccg gaccagctcc cagtcgtccc gggcggtcct ctccggcacc cacttcccga
    10621 tcccgggatc caggatgaag tcatcgatgt cgtactccat gcaccgggtc accaccagct
    10681 ggagcgcgtc cagggtcgcc tggagaccga tggcatcccc aggaacctcc cgggaggcca
    10741 taaggatcgc ggggagcccg gagtccctta cccggagagc gaaccgggga tccgagaggc
    10801 ctccgatgtc gttcaccgca tggatgtcgt accggaggca tgccgcgagc acctcgggct
    10861 ccctcgtgtc caccgagacc gtgacgccgg tcccctcgag ggccgagagg gccgcaacca
    10921 tccgctccct ctcgacctcc ggcgaaagag ggggggatcc cggccccgtt gcccgggccc
    10981 ccacgtccac gaggtcagcc ccgtccgcga ccatggtgag ggccttttcc cccgccttcc
    11041 cgggagggac atacgacccc cggtaaaagg actctgggct gcagttaatc acgcccatga
    11101 gccggacggg cgagccatcc ccgatggaga ggccccggat cacgcaggcc tgcatctcct
    11161 ctcccgtgcc gccgcgaggg tgttctccat gagcatggcg atggtcatcg gccccacgcc
    11221 gcccgggacg ggggtgatgg cccgggcgac cggttccacc gacgggaagt cgagatcccc
    11281 gaccggcttc ccgccgacca tggtgatccc cacatcgacc gcgacaacgc cctttttgac
    11341 catgtccccc gtgatgaacc cgggcttccc cacggccgag acgaggatgt ccgcagaccc
    11401 cgtctttccg gcgaggtccc gcgtcttcga gtggcagatg gtgaccgtcg atccggcatt
    11461 gaggaggagg gcggccatgg gccggcccac ctcgagagac ctccccacca ccacggcatc
    11521 ggccccctcg accgggatgc catactcaga aagaagggtc atgaccccga ggggcgtgca
    11581 ggggacgaag gcgggattcc ccgagaaaag ccgtcccagg gagaaggggg tgaacccgtc
    11641 cacgtccttc tcgggcggga tggcggcgat gatccgtctt tcatcgagat gccgcggaag
    11701 ggggagctgg acgaggatcc cgtcgatgga gggattctcc ccgagcgccc tgatttcccc
    11761 ctcgacctcg cccgaggccg ccccctccaa gagctccacc gagacggatc cgatcccgac
    11821 ctgctcgcat gtccggtgct tcatccttac gtagagctgt gatcccggat cctttcccac
    11881 catgaccgtg gcaagccgcg gggtgatccc ggaggccctg atctgctcct tcaggccctc
    11941 gagacgctta tcggcggttg cccggccgtc aaggatcata tcactccggg tagatgggga
    12001 accggctcgc catggcgatg acctcctcct tcacctgcac cagggccttc tcatccttca
    12061 catccctgat gacatgcagg atcttcgccc cgatgaacct catctccggc tccttcatgc
    12121 cccgtgaggt aaccgccggg gtaccgatcc ggaggccgct cgtgacaaag gggctccggg
    12181 tctcccgggg gatggtgttc ttgttcacgg tgatcccggc gcagccgagg gcgttctcgg
    12241 cctcgaggcc ggtgatgccc ttgtccgaga ggtcgatgag gaggaggtgg ttgtcggtgc
    12301 ctcccgagac gaggcgcagg ccgccgtcca tgagggtctg ggcgaggacc cggcagttct
    12361 tcacgatctg ccggttgtag tcggtgaact ggggccttag cgcctccttg aagcagaccg
    12421 cctttgccgc gatggtgtgc atgagggggc cgccctgcat ccccgggaat accgctttat
    12481 ccaccgcctg ggcgaactcc ttcccgcaca tgatggcccc gccccggggg ccgcggaggg
    12541 tcttgtgggt cgtggtcgtc gtgaagtgcg ccacgttcac cgaggtcggg tgcacgccgg
    12601 tggcgcagag cccggcgata tgggcgatgt ctgccacgca ttttgcatcc acggcatccg
    12661 cgatctcccg gaaggctttg aagtcgatgg tcctcgggta ggcgctcgcc ccgcagacga
    12721 tgacattcgg cttctccttc cgtgcgatgg actcgatgac cccgtaatcg agggtctcgg
    12781 tctccaggtc cactccgtac ggcacgatcc ggaagagctg gcccgagaag ttcaccggtg
    12841 agccgtggga gaggtggccc ccctgggaga ggctcatgga catgagggtc cccccgggct
    12901 tcatcgcggc gaagtaggcc gccatgttcg cctgggtgcc cgagtggggc tggacgttcg
    12961 catgctcggc cccgaagagc tgcttcagcc ggtcccgggc caggttctcg gccacgtcgt
    13021 agtacccgca tccgccgtag taccgcttgc cagggtatcc ctcggcgtac ttgttcgtca
    13081 tgatggatcc ctgcgcctcg aggacggcgc gggacacgat gttttcggag gcgatgagct
    13141 ccagcccgtt cagctggcgc agacgctcct tctcgatcac gtcggcgatc tccggatcag
    13201 tcctggcaag ctcggacatg cattagagaa aggggagtcc ttgggcatga gccttttcct
    13261 taatgcgacg ttcccgtcag ggaacggagc tggagcaccg cgaagcggtg cgataatacg
    13321 acgttcccgt taggtccggg gtgaacacaa aggatatagg ttacctgaaa gataaattcc
    13381 ttagaaatct tcggtttccc gtgaggtacg gggagatcag gaaggttcga cggtcccatc
    13441 aggggactag aatgagaaag gcgtcagcct ttcgggattc gactgttccg acaggaacaa
    13501 gagaacgaga aaggcatcag cctttcgagg ttccatacag gatacggagt ggggtacatg
    13561 aacggggata tgagggttcc acaggccggg agcgaccggg tgagcgagct ggagaacaag
    13621 gtccgggaga tggaggccct catcaagggg ctcacgaacg agctcctgga cctcaagtcc
    13681 atcgtcgaga gactgaacag caggttcgag gagaagcggc ctccgcttct cacgcggccc
    13741 cagggaaggg tgagcgtctc cccgagggga cagccgggcg agggcgcccc ggcgagcgag
    13801 ggggagctcg cccggatcat gcagtccgat ggaaccatga aggaggagcg gaggaaggac
    13861 tcggactaca tcgtcgcctc cgcaaagacg gcacaggccc tccggagcaa gggcaagggg
    13921 gagccgtcca agggcaaggg atccaacgcc ctcatctacg cggtcgagga ggaagacgaa
    13981 aaatccaagc agtgagtatc ccgataccat aagccggaac gacagaccgt ggggcctgcc
    14041 ttgcatatca ccgagatcga gatcgataac ttcaagtcct tcgggcgaaa gaccaccatc
    14101 ccttttttcg agcacttcac cgtcatctcg ggcccgaacg gctccgggaa gagtaacatc
    14161 atcgatgcca tcctcttttc cctcgccctc tcctcgtcga ggaccctccg ggccgagaag
    14221 ctcaccgacc tgatcaacct gaacaccggg aagaacaccg ccgaggtcgc catccggttc
    14281 tctgacgaaa ccgtggtgag gaggcggatc cggcgcaccc cgcacgggta ctacagctac
    14341 aactacctga acaaccggct ctgcaaacag acggacatca cggagttcct ggggaaggtc
    14401 gggatcaagc ccgaggggta caacgtcgtg atgcaggggg atatcacccg gatcatggag
    14461 atgtccgaca ccgagcgccg caagatcctg gacgagatcg ccggggtcgc cgagttcgac
    14521 tccaagaagg aggccgccct ccgggagctc gaggtggtga gggagcggat cgaccgggag
    14581 gagctcatgc tccacgagct cgacacccgg ctccaggagc tcgaggccga gcgggaccgg
    14641 gcggtggagt accgggccct cctcgatacg atccaccggt tcgaggtctg ccgctcggcc
    14701 gcggagctcc gggagaggga aaaggagctt ctcgccctcc gggagctctc ggccgagcag
    14761 gggaaggccc tcgagaaggc aaagggtgcg gccgcggacg tggccggtgc catcgggaaa
    14821 ctgaacggcg agctcgggga gctcgacctc cagatcaacg agaagagcgg gcctgagtac
    14881 ctctccctcg tctccggcct cgaggccgag aaggggaaga tcaccctcgc ggagcagacc
    14941 atcgggcgca tctcgaagga gaaggaggga aacctggagg agatcaaccg gatctacctc
    15001 gaccagaagc gggccgaggc caaggtcacc gagggaaccc aggccatccg gaccctgtcc
    15061 atcgaccggg cgaacctggc catggaggcc gggtccctcc gggggaagtt cgaggtcttg
    15121 gaccgggacg tgaagtcccg gacctcgaac gtcgagagct cccgggaccg gctcttctcc
    15181 ctcatggagg aggtggagaa gaagaagact gcccgggccg aggtgatcca caagaaggat
    15241 ctcctcatcg agaagagccg gggcaggacc tccgagcgcg aacgccttca gaaggaggtg
    15301 gaggcggccg aggccgagat ggccgagaag gaccggcagg tcgccgagtg cgggacctcg
    15361 gtcgcggacc gggaggcggg gaagcagggc ctggagcggg agatcgccgc cgccgaggga
    15421 cggctcttcg ccctccgggc cgaggccgag cggctggaga ccgaaggccg ggcaaaggag
    15481 cgggagctcg cccggctcga ggcccagcag caggccgggg gcgaggggac cgagcggggg
    15541 ctcgaggcgg tccggggcct ggacggcgta cacgggacca tccgggagct cgggaaggcg
    15601 gacccggagt acgccaccgc cctctcggtc gcggccggcg gaaggatccg gaacatcgtg
    15661 gtggacaacg accgggtcgc ggccgatgcc attaccctcc tcaaggagca gaagctcggc
    15721 cggttcacct tcctccccct gaagaacctg aagaacgtga acctcccgcc gctcgaggcg
    15781 cggcccgggg tcatcgacta tgccgtgaac ctgatccact acgacccggt gtacgccccc
    15841 gcgttccgga tcgtcttcgg ccggaccgtt gtcgtcgaca ccctggaccg ggcccggaag
    15901 ctcctcggga aataccggat ggtgaccctc gagggcgagc tcctggaaga gagcggggcc
    15961 atgaccggcg ggtccctgaa gaaggtgaag ggcttcggta tcgccgccga ggatgacatc
    16021 gcccggctcc gggccgagat ccagggcctc gccgccgaga ccgcggaggt gagggcgggc
    16081 atccagaggg aggaggcccg gtccacggat ctccgggcac ggcggacgac cctcgacgag
    16141 gagatggccc ggttccggat ccttgccgag gagtacggca agaggaggga cgccctcacc
    16201 gaggaaaggg cggcaaccgc cgcggccctc gcaaagatgg acgaggagac gggtggcggg
    16261 gcgcaggagc tcgcggcctg cgagcaggag ctcgaggaga tgacccggga gatcagccgg
    16321 gtcacgggcg aggtcgagga gctgaagagg gccctggacg acgacgccct ccccggcctc
    16381 gtccaggaga tggagcgcct ccgcaccgag gccgacgagg ttgagaagcg gctccggaac
    16441 aaggacgccg agatctcggg cgcccagcag gagcggtcgt acttcacgaa gcgggtggag
    16501 gagctctccg ccgagaagga gcgggtcgcg gggaagaaca gggagatcga tgccaccgtc
    16561 gcctcgtcga aggaagagat cgagcgggca aagggggcca tcgtctccct cgaggaacgg
    16621 ctctcggtct tctcgaagga gctctcgggc ctccgggagg cccgggacaa gctcaccgag
    16681 aagatccgga ccgcggaacg gaagagggcc gacagggagg cggggatcga gcggatcgcc
    16741 ctccagatct cggccctcga ggagaaggag aaggccatcg ccccggccct cgaggccctg
    16801 aaggtccagg ccgcggggat cgagaccgag ctctcgctcc aggagatcga ggagggactc
    16861 tccgaggcga agaagggcgt cgagggaatg ggggcggtga acatgctcgc catcgaggag
    16921 tacgagcgga ccacggcccg ggtgaaggaa cggaaggagc ggaaggagat cctctcccgg
    16981 gagcgggcga gcatcctcga acggatcgag cagtacgaga agatgaagca cagcgcgttt
    17041 tccacggcgt tttccgccat cgacggcaac ttccgggaga tcttcgcccg cctcacccgg
    17101 gggagcggcc acctggtcct cgagaacgag gaggacccct tcgccggcgg cctcaccttc
    17161 gccgtccagc cccgggacaa gaaggtccac ctcctctcgg ccctctcggg tggggaaaag
    17221 tccctcacca ccctcgcctt catcttctcc atccagaagt acatgcctgc ccccttctac
    17281 gccctcgacg aaatcgacat gttcctggac gggtacaacg tcgagcagat cgcaaacatg
    17341 atcaaggagc tctcggcgaa cgcccagttc gtcatcgtct cgctccggaa gcccatgatc
    17401 gatagcgccg accggatcgt gggggtgacc ctgcgggagg acaagtcgtc cctcgtcacc
    17461 ggggtgaagg ccagtgggta gggctccggc gggattcggc tggacgtcac cgggggtgaa
    17521 ggccagtggg gagacccagg cggtgcccgg ctggacttca ccggggatga atgcccatgg
    17581 atgaggagcc ggtcgagatc ctggtgggcc ttgccgagcg gggggagatc gacccctgga
    17641 acatcgatat cctggaggtg acggaccgtt tcctcaacga actcgatcgg ctccggcagc
    17701 tcgacctgcg ggtctcgggg aggaccctct tctatgccgc gaccctcctt cggctgaagt
    17761 ccgagttcct cgtcgaaccc gatgcgacgg gtgaggagga cgagttcggc cccgaggagg
    17821 agccccccga gggcttcgat atccacgagt ggctcggccc catcgagcgg ctcgagcggg
    17881 agatccggcg ccgcatcgag cggaagaacc tccgcacccg gccggtcacc ctctacgagc
    17941 tcatccggat gctccgcacc gccgaacgcg aggagcaccg gagacggcgc acccgcaccg
    18001 tcactcccga gttcatcccg gagacggcgg atgtggtctc ggtcgcccat gacgaggggt
    18061 accaggagac ggcaggcgac gtctggaaga ggtgggtcga gctggccgca tcacgggagg
    18121 agatccggct cggggagctc tgccagaccc tggacagaaa gatcgccgac gtgtacatac
    18181 ccctcctgtt cctcaacctc gatgggaagg tggagatatc gcaggagacc tttttcgagg
    18241 aggtgttcat ccggcgggtg ccggagaagg cccatgacag agagccggtg aaaaatgctg
    18301 agaacggaaa gaggaaaacg aggaagaggg agcaggcggc agagtgacgg gaacacatcg
    18361 caccgcttcg caccgcttcc gcggtgctca agttccgctt cctcgcaaca ctccatgtta
    18421 cttgagctcc gcttccttgg gaagcgtcgc acttcggaaa cgtcgctctt caccgtgctc
    18481 cagctccgtt gccctgatgg gaacgtcgct ctgcgcgccc caccgctccc gtgatgatcc
    18541 tccggccggg agcttcacat tcccgggaag ttcagcattt attgaaccgg atttttggcc
    18601 gttttcccgt cggccgcaag ctatatatcc cgacacatcc atcttattga ggtcatgggg
    18661 gacggaccgg ggataaaggg
//
LOCUS       DATGDI010000124         1543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_303076, whole genome shotgun sequence.
ACCESSION   DATGDI010000124 DATGDI010000000
VERSION     DATGDI010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303076"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VKO45_04865"
     CDS             <1..683
                     /locus_tag="VKO45_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA05239.1"
                     /translation="ATPEQVMQAIRVVNEVGGARREGIPELLPGLNFIIGLAGETEET
                     FRLNREFLEGLLAGGLLVRRVNIRKLMAFPGTRAYTENTLGRHERLFRRFKEEVRRDF
                     DRPMIARVFPLGTVLRQVIVEQPGPVSYGRQMGSYPVLVGIPLVLAERRVLDTVVVDW
                     GMRSITALPYPVEVNLLPFQALAWLPGIGRKRAGALLAARPFRDLAAFRAVAGETPLD
                     GALSFRTD"
     gene            complement(680..>1543)
                     /locus_tag="VKO45_04870"
     CDS             complement(680..>1543)
                     /locus_tag="VKO45_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA05240.1"
                     /translation="IKINSITLLILGGVSSIENEVPDPRDELPMSLAGPLTSLGLAVI
                     SWLAVLAGENLIPTALAPVGGVVVFLFGYLALLNLILFAFNLLPAFPMDGGRVLRAWL
                     AKRMPLPRATKIAADVGKVFAFLFVIFGVLTLNFILVIIGIFIYLGADQEFYAIRSTV
                     LLKDVAVSDVMSSSLITVPSTMPVPEVIRLMYATKHLGFPVVDAGALAGMVTLEDVHR
                     TSEIDRDAKQVKDIMSRNLITLPPTAMLTEAFRLMSRHNIGRIPIVADGKLLGIVTRT
                     DILKVMELREA"
BASE COUNT          265 a          463 c          568 g          247 t
ORIGIN      
        1 aggcgacccc ggagcaggtg atgcaggcca tccgggtggt gaacgaggtg ggcggggcca
       61 ggcgggaggg gataccggag ctcctcccgg gcctgaattt catcatcggc ctcgccggcg
      121 aaacggagga gaccttccgg ctgaaccggg agttcctcga agggctcctt gccgggggac
      181 tcctcgtccg gcgggtgaac atccggaagc tcatggcctt cccggggacc cgggcgtaca
      241 cggagaacac cctcgggaga catgagcggc tcttccggag gttcaaggag gaggtcagga
      301 gggacttcga ccggccgatg atcgcgaggg tattcccgct cgggacggtc ctcaggcagg
      361 tcatcgtgga gcagcccggc cctgtctcgt acggccggca gatggggtcc tacccggtcc
      421 tcgtggggat cccgctcgtc ctcgcggaac ggagggtgct cgacaccgtt gtcgtggact
      481 gggggatgcg gtccatcacc gccctcccgt atccggtgga ggtgaacctt ctccccttcc
      541 aggccctcgc atggctcccg gggatcggga ggaagcgggc cggtgccctc ctcgccgccc
      601 ggccctttcg ggacctcgcg gccttccggg ccgtggccgg ggagactccc ctcgacgggg
      661 ccctctcctt ccggacggat taggcctcgc ggagctccat caccttcagg atgtccgtcc
      721 gggtgacgat gcccaggagc ttcccgtccg cgacgatggg gatcctcccg atgttgtgcc
      781 tggacatgag ccggaaggcc tcggtaagca tcgccgtcgg ggggagggtg atgaggttcc
      841 gggacatgat gtccttcacc tgctttgcgt cccggtcgat ctccgaggtg cggtggacgt
      901 cctcgagggt gaccatgccc gcgagcgccc cggcgtccac gacggggaaa cccaggtgct
      961 tcgtcgcgta catgagccgg atcacctcgg gcacgggcat cgtggagggg acggtgatga
     1021 gggagctgga catgacatcc gataccgcaa cgtccttgag gagcacggtg gaccggatgg
     1081 cgtagaactc ctggtctgcc ccgaggtaga tgaagatccc gatgatgacg aggatgaagt
     1141 tcagggtgag gaccccgaag atgacgaaga ggaaggcaaa gaccttcccc acgtccgcgg
     1201 cgatcttcgt cgccctcggg aggggcatcc gcttcgcgag ccatgcccgg agcaccctcc
     1261 ccccgtccat ggggaaggcc gggaggaggt tgaaggcaaa gaggatcagg ttgaggaggg
     1321 cgaggtaccc gaagaggaag accacgaccc cgccgaccgg ggcgagagcg gtgggaatca
     1381 gattttcccc cgcgagcact gcgagccagg agatgacggc gaggccgagg gaagtgaggg
     1441 gcccggcgag ggacatgggg agctcgtccc ggggatcggg gacctcgttc tcgatggagg
     1501 agacgccccc gaggatgagg agggtgatgc tgttgatctt gat
//
LOCUS       DATGDI010000125         5614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_245032, whole genome shotgun sequence.
ACCESSION   DATGDI010000125 DATGDI010000000
VERSION     DATGDI010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245032"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VKO45_04875"
     CDS             complement(<1..677)
                     /locus_tag="VKO45_04875"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HMA05241.1"
                     /translation="MTFLEDAKRGIVGDEIRTVARDEGVTEDFVRRGMAAGRIVIPIS
                     PYRKVKFCGIGEGLRTKVNASVGTSSDIVDIDMELEKVRQAELAGADTIMELSTGGDF
                     AEIRRRVCGATSLSVGSVPLYQAFIEAAIRDGAVVHMKEDDLFRITAEQAKLGTNFMA
                     IHTGINLETMERLKNQGRTCGLVSRGGAFLTAWMLHNKKENPLYSEFDYLLEILKEHE
                     VTLSMGNG"
     gene            1277..2005
                     /locus_tag="VKO45_04880"
     CDS             1277..2005
                     /locus_tag="VKO45_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HMA05242.1"
                     /translation="MKVNFGGFVPLSTVDWRGRAVCTVFLRGCPVRCSYCHNRSIQDG
                     EDFRELEEVTALMRSSRILVSGVVFSGGEPTMQPEPLKALARASRAMGLLVGLQTNGV
                     FPKVLASLISEKLVDRVALDIKARWERYDNLLKEKYSGPIKESLALCRDAKLAGDLPE
                     FEVVVTLFRGYEDEVPYIARVADGVDLVLQQGMDGGVKPLSSEELRKVADRVGRVVKI
                     RTREEGEVVYAGDRGRRVSGERQG"
     gene            1959..2507
                     /locus_tag="VKO45_04885"
     CDS             1959..2507
                     /locus_tag="VKO45_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMA05243.1"
                     /translation="MRVIGVVGYPASGKGEFSRIATESGIPVVVMGDVIRRKATEAGL
                     GTSDAALGEMAVRLRKEQGMDAVALATIPAVEALGAPLVIIDGIRGDAEVEVFRRHFP
                     VFLLVGIVSSFETRLSRSSARGRSDDLTRAEDLRNRDRREEGFGLARALAGADRVIGN
                     DGSLGDFEERARALLDRIRREA"
     gene            2504..3277
                     /locus_tag="VKO45_04890"
     CDS             2504..3277
                     /locus_tag="VKO45_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HMA05244.1"
                     /translation="MSLTPFFSASSKAWEDRRWVYSLEGIGYRGWEVMADGASSFERP
                     GIVKEVTEIAASTRLALSVHAPYSDLNLASLNHPIWRESVRQVLACIEGAADLTDRVT
                     VHPGYLSPMGKLLPEKVWALQKSALSEIGKYAADRGVRVFLENMGGLKEVLCRFPEEI
                     LGMIEDAGGTGFTLDIGHAHTVGKVREFLAVLPSAHHIHVHDNHGTADEHLALGEGTI
                     PWEEVGRAVARDYRGIVVVEGRSLGEAKKSIAVFRRCFV"
     gene            3274..4212
                     /gene="rnz"
                     /locus_tag="VKO45_04895"
     CDS             3274..4212
                     /gene="rnz"
                     /locus_tag="VKO45_04895"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HMA05245.1"
                     /translation="MTGETLHVHFLGTAGALPTPQRNLPCIMVRRGSDTLLFDCGEGA
                     QQQMMRARTGFTVNAVFITHWHADHFLGAFGLAQTLSFMGRTEPLTLYGPPGVHEFAG
                     LVAQLCRNTLRFPVESVEMRGGSVVEFGGYAVHAFATQHGIQSLGYVLAEDPRPGRFD
                     RERAVSLGVNPGPLFGRLQRGETVRVTTNGQERVVRPDEVMGPPRAGRRICYTGDTRP
                     VEDRIREIAGGADLLIHDATFDDRDRERAEEVHHSTAGEAGAAAAALGVRTLALVHIS
                     PRYTTTVAHVEDARRHFTGEVIAPADLVMLDIPFRD"
     gene            4319..>5614
                     /locus_tag="VKO45_04900"
     CDS             4319..>5614
                     /locus_tag="VKO45_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HMA05246.1"
                     /translation="MEGDTKVIVYRLGANCDIGEVEEGKIYSGKVQGFANFGAFVLLN
                     DRVKGLVHKSNIKQRHKEQDPILVLVKGIRPNGNIDLEEVTPSVFHTEMVVKKSAPVR
                     LKDLGKSIGRVVRIQGEVSQVKQTSGPTIFTLVDGDGIANTAAFVEAGVRAYPEVQLG
                     DIVSIMGEVMQRNSQLQVEVSSLAVLTGDEALHVREEIDVTLEARAEPPELPLLVESP
                     VLSALVPAMRIVARRIRKAVFMGQPILVRHHADADGISAAVALEQAVVALIRENGGDY
                     DAASFLFKRSPSKAPFYEIEDITRDLDFALKDLVRYGQKLPLIILMDNGSTEEDIPSL
                     RMAAVYDLDMLVVDHHHPDEVVDPYLEGHVNPYHVGGDFGVTSGMLGVELARLVYPGV
                     EGAIRHIAAVSAVGDRSEAPERARFLAIAGEAYPEAHCRD"
BASE COUNT          952 a         1838 c         1879 g          945 t
ORIGIN      
        1 ccgttcccca tggagagggt gacctcgtgc tccttcagga tctcgaggag gtagtcgaac
       61 tccgagtaga ggggattctc cttcttgttg tggagcatcc acgccgtgag gaaggcgccg
      121 ccccgggaga cgagcccgca ggtcctcccc tggttcttca gccgctccat ggtctccagg
      181 ttgatccccg tgtggatggc catgaagttc gtgccgagct tcgcctgctc ggcggtgatc
      241 cggaagaggt cgtcctcctt catgtggacc acggccccgt ccctgatggc ggcctcgatg
      301 aaggcctggt agagaggcac cgagcccacc gagaggctcg tcgccccgca gaccctccgc
      361 cggatctcgg cgaaatcccc gccggtggag agctccatga tggtgtcggc gcctgcgagc
      421 tccgcctgcc ggaccttctc gagctccatg tcgatgtcca cgatgtccga ggaggtgcct
      481 accgaggcgt tcaccttggt ccgcagcccc tcgccgatcc cgcagaactt caccttccgg
      541 tacggggaga tggggatgac gatcctccct gccgccatcc ccctccggac aaagtcctcg
      601 gtgaccccct cgtcccgggc caccgtccgg atctcgtcgc cgacgatccc gcgcttcgca
      661 tcctcaagaa atgtcatatc ccaccactag gcgaagcgac cagataaagg cttgttttcc
      721 agaaatctaa ataaatctgg cttgtctgct tcgcaagtaa agttgatttg cgttacggca
      781 aacgaggttt attaaaagga aatcttcttt cccatgatat tcgaagcccc caaggacttg
      841 atggatctat cgtttttcga taatccggat gacgctcctg ggggcttcac ctgatggctc
      901 gcccccgccg ctgtgacgtc ccccaattgc gataggccct tcaattggag gaagaaggtt
      961 gatgatattc gcgaaaactc tcgcgggaat ccccatcaac ggagggggat ggccgcgccg
     1021 gggggcggta gccccccaag agcctgacaa cgaaaccaag attgcctgac ttagtcaaag
     1081 cttcggttcg gtaaggtcac cgatcattcg ttgaggtgta acaccacagg agcccgccgc
     1141 gaaaatactg atcgccatta cgttcctgct tgggctccgg atgaaattct attaccgtaa
     1201 ctgatccaca cctccgcttc ggatcgcata gcttcatgaa acctgccgcc gcacgctctg
     1261 atcagccggt gattctctga aggtcaactt cgggggcttc gtaccccttt ccacggtgga
     1321 ctggagggga cgtgccgtct gcacggtctt cctccggggc tgcccggtcc ggtgctccta
     1381 ctgccataac cgttccatcc aggatgggga ggacttccgg gagctggagg aggtgacggc
     1441 cctcatgcga tcctcacgga tcctcgtctc cggggtggtc ttctcgggag gcgagccgac
     1501 gatgcagccc gagcccctga aggccctggc ccgggcctcc cgggccatgg ggctcctcgt
     1561 cgggctccag acgaacgggg tcttcccgaa ggtcctcgcc tccctcatct cggaaaagct
     1621 cgtggaccgg gtggccctgg acatcaaggc ccggtgggag cggtacgaca acctcctgaa
     1681 ggagaagtac tcggggccca tcaaggagtc cctcgccctc tgccgggatg caaagctcgc
     1741 gggcgacctc ccggagttcg aggtggtggt gaccctcttc cgggggtacg aggacgaggt
     1801 tccctacatc gcccgggttg ccgatggggt ggacctcgtt ctccagcagg ggatggatgg
     1861 cggggtgaag cccctctcgt ccgaggagct ccggaaggtc gcggaccgcg tcggccgggt
     1921 ggtgaagatc cggacccggg aggaagggga ggtggtgtat gcgggtgatc ggggtcgtcg
     1981 ggtatccggc gagcggcaag ggtgagttct cccggatcgc caccgaatcg ggaatcccgg
     2041 tggtggtcat gggggacgtg atccggcgga aggccacaga ggcgggcctc gggacctcgg
     2101 atgccgccct cggggagatg gccgtccggc tccggaagga acagggcatg gacgcggtcg
     2161 ccctcgccac tatcccggcg gtagaggccc tcggtgcccc cctggtaatc atcgatggga
     2221 tccggggaga tgccgaggtg gaggtcttcc ggcgacactt ccccgtcttc ctcctcgtcg
     2281 ggatcgtctc ctccttcgag acgcgcctct cgaggtcctc ggcaaggggg aggagcgatg
     2341 acctgacccg ggccgaagac ctgaggaacc gggaccggcg cgaggagggc ttcggcctcg
     2401 cccgggccct cgccggggcg gaccgggtca tcgggaacga cgggtccctc ggggacttcg
     2461 aagagagggc gagggccctc ctcgaccgga tccggaggga agcgtgagcc tcaccccctt
     2521 cttctcggcc tcctccaagg catgggagga ccggcggtgg gtctactccc tggagggcat
     2581 cggttaccgg ggctgggagg tgatggccga cggggcctcc tccttcgagc ggcccgggat
     2641 cgtgaaggag gtcaccgaga tcgcggcatc cacccgcctc gccctctcgg tccatgctcc
     2701 ctacagcgac ctgaacctcg cctccctcaa ccaccccatc tggagggagt cggtgcgcca
     2761 ggtcctcgcc tgtatcgagg gggccgcgga tctcaccgac cgggtcaccg tgcatcccgg
     2821 gtacctctcg cccatgggca agctcctgcc cgagaaggtc tgggccctcc agaagtccgc
     2881 tctctccgag atcgggaaat atgccgcgga ccggggggtc cgggtcttcc tggagaatat
     2941 ggggggcctc aaggaggtcc tctgccggtt ccccgaagag atcctcggga tgatcgagga
     3001 cgccggcggg accgggttca ccctggacat cgggcatgcc catacggtcg ggaaggtgag
     3061 ggagttcctc gcggtccttc cctcggcaca ccacatccac gtccatgaca accacggcac
     3121 cgccgacgag catctcgccc tcggagaggg gaccatcccc tgggaggagg tcgggagggc
     3181 cgtggcccgg gactaccggg ggatcgtcgt cgtggaggga aggagcctcg gggaggcgaa
     3241 aaagagcatc gccgttttcc ggaggtgttt cgtctgaccg gcgagaccct ccatgtccac
     3301 ttcctgggga cggcgggggc actcccgacg ccccagcgga acctcccctg catcatggtg
     3361 aggaggggct ccgacaccct cctcttcgac tgcggcgagg gcgcccagca gcagatgatg
     3421 cgtgcccgca ccggcttcac cgtgaacgcg gtcttcatca cccactggca tgccgaccac
     3481 ttcctcgggg ccttcggcct cgcccagacc ctctccttca tggggaggac cgagcccctc
     3541 accctctacg gcccgccggg ggtccacgag ttcgccgggc tcgtcgcgca gctctgccgg
     3601 aacaccctcc gcttccctgt cgagtcggtg gagatgcggg ggggctcggt ggtggagttc
     3661 ggcggctacg cggtccatgc cttcgccacc cagcacggca tccagtccct cggctacgtc
     3721 ctcgccgagg acccgaggcc ggggcggttc gaccgggaga gggcggtctc cctcggggtg
     3781 aaccccggcc ccctcttcgg caggctccag cggggggaga ccgtccgggt cacgacgaac
     3841 gggcaggagc gggtggtccg gcccgacgag gtgatggggc ccccgcgggc gggccggagg
     3901 atctgctaca cgggggacac gcggccggtt gaggaccgga tccgggagat cgccggcggg
     3961 gcagacctcc tgatccacga tgccaccttc gatgatcggg accgggaacg ggcagaggag
     4021 gtccaccact ccacggccgg cgaggcgggg gcggcggccg cagccctcgg ggtgaggacc
     4081 ctcgccctcg tccacatcag cccgaggtac acgacgacgg tggcccatgt cgaggatgcg
     4141 aggaggcact tcaccggcga ggtcatcgca cccgccgacc tggtcatgct cgacatcccc
     4201 ttcagggact gaaagaacgg cacgggacta cccataagag ggaggacggc gattactagg
     4261 taggaaggat ctccggcccc ggccgcgatg gggggcgggg aggaacacga gggtgcacat
     4321 ggaaggggat accaaggtca ttgtctaccg gctgggcgcg aactgcgaca tcggggaggt
     4381 cgaggagggg aagatctaca gcgggaaggt ccagggcttc gcgaacttcg gggccttcgt
     4441 cctcctgaac gaccgggtga aggggctcgt ccataagtcg aacatcaagc agaggcacaa
     4501 ggagcaggac cccatcctcg tccttgtcaa aggcatccgg ccgaacggca acatcgacct
     4561 cgaggaggtg accccctcgg tcttccacac cgagatggtg gtgaagaagt ctgccccggt
     4621 caggctgaag gacctcggaa aatccatcgg gagggtcgtc cggatccagg gagaggtctc
     4681 ccaggtcaag cagacgagcg gcccgaccat cttcaccctc gtggacggcg acgggatcgc
     4741 gaacacggcc gcctttgtcg aggcaggggt ccgggcctac ccggaggtcc agctcgggga
     4801 catcgtctcc atcatggggg aggtgatgca gaggaactcc cagctgcagg tcgaggtttc
     4861 ctccctcgcg gtcctcaccg gggatgaggc cctccacgtg cgggaggaga tcgatgtcac
     4921 cctcgaggcg agggccgagc ctcccgagct cccgctcctc gtggagagcc cggtcctctc
     4981 cgccctcgtc cccgccatgc gcatcgtggc ccggaggatc cggaaggccg tgttcatggg
     5041 gcagcccatc ctcgtccggc accatgccga tgccgacggg atctccgcgg ccgtcgccct
     5101 cgaacaggcg gtggtggccc tcatccggga gaacggcggg gactatgacg ccgcctcctt
     5161 cctcttcaag cggtccccga gcaaggcccc cttctacgag atcgaggaca tcacccggga
     5221 cctggacttc gcgttaaagg acctcgtccg gtacggccag aagctccccc tcatcatcct
     5281 catggacaac gggtccaccg aggaggacat cccctccctc cggatggctg ccgtctacga
     5341 cctggacatg ctcgtcgtgg accaccacca cccggacgag gtggtggacc cctacctcga
     5401 ggggcacgtg aacccgtacc acgtcggggg cgacttcggg gtgacgagcg gcatgctcgg
     5461 ggtggagctc gcccgtctcg tctacccggg cgtcgagggg gccatccgtc acatcgcggc
     5521 ggtctcggcg gtgggggacc ggtccgaggc cccggagcgg gcacggttcc tcgccatcgc
     5581 aggcgaggcc tacccggagg cgcactgcag ggac
//
LOCUS       DATGDI010000126         1608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_70654, whole genome shotgun sequence.
ACCESSION   DATGDI010000126 DATGDI010000000
VERSION     DATGDI010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70654"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VKO45_04905"
     CDS             complement(<1..168)
                     /locus_tag="VKO45_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HMA05247.1"
                     /translation="MRHEVRFSGFGGQGIILAAVILGRAAALYDGKHAVQTQVYGPEA
                     RGGASMSAVIID"
     gene            complement(165..953)
                     /locus_tag="VKO45_04910"
     CDS             complement(165..953)
                     /locus_tag="VKO45_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMA05248.1"
                     /translation="MSFRDWYRADRLPTIYCAGCGNGTVINCALHAVDSLGWKWEETV
                     FVSGIGCSSRAPGYIRTDSLHTTHGRALAFATGIKLAKPELHVVVFTGDGDLAAIGGN
                     HFIHACRRNVDMTVICMNNWIYGMTGGQGSPTTPRGAITTTTPYGSGEGVFDLAALAV
                     AAGANHVARWTSYHVKELTNAIQAGLQTPGFSLVEVLTQCPTAYGRRNKLREVPDMIE
                     YLKTHAILLPKYNRLVAEGKPVPEDMFTVGEFVRRNRPAMGVPP"
     gene            complement(950..>1608)
                     /locus_tag="VKO45_04915"
     CDS             complement(950..>1608)
                     /locus_tag="VKO45_04915"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HMA05249.1"
                     /translation="LTVRCFNLADRYRVPVFLMADETIGHMREKILIPDTVERVGRKP
                     FVPGTPPFRATDPDLVPAFPQFGTGQHVHVTGLTHDERGYPAATNPRLHEKLVMRLVH
                     KVELARKEMADYVVTNPGAEQVFISYGAPARSVFQLLHDRPKEEIGHLRLRVVWPFPE
                     FALGEFPEAKAFLVPEMNLGQVAREIERHTDVPVIPIPRLGGALHPPGMLAKALEASR
                     "
BASE COUNT          244 a          532 c          572 g          260 t
ORIGIN      
        1 gtcgatgatc accgcggaca tggaggcccc gcccctggcc tcggggccgt acacctgggt
       61 ctggaccgcg tgcttcccgt cgtagagggc cgcagcccgc ccgaggatga ccgcggcgag
      121 gatgatcccc tgcccgccga agcccgagaa ccggacctcg tgcctcaagg cggcaccccc
      181 atggccggcc ggttcctgcg gacgaactcc ccgacggtga acatgtcctc agggacgggc
      241 ttcccctcgg cgacgagccg gttgtacttc gggaggagga tggcatgggt cttcaggtac
      301 tcgatcatgt ccgggacctc cctgagcttg ttcctcctcc cgtacgccgt ggggcactgg
      361 gtgaggacct cgacgaggga gaagcccggg gtctggaggc cggcctggat ggcattcgtg
      421 agctccttca catggtacga ggtccaccgg gccacgtggt tcgcccccgc tgccacggcg
      481 agggccgcga ggtcgaagac cccctcgccc gagccatagg gggtcgtggt ggtgatggcc
      541 ccccggggcg tggtggggga gccctgccca ccggtcatcc cgtagatcca gttgttcatg
      601 cagatgaccg tcatgtccac gttccgccgg caggcatgga tgaagtggtt cccgccgatg
      661 gcggcgaggt cgccgtcccc ggtgaagacg acgacgtgga gctccggctt cgcgagcttg
      721 atccccgtgg caaaggcgag ggcccggcca tgggtggtgt ggagggagtc ggtccggata
      781 tacccgggtg ccctcgacga gcagccgatc cccgagacga agaccgtctc ctcccacttc
      841 cagccgaggc tgtccacggc atggagggcg cagttgatga ccgtcccgtt cccgcacccg
      901 gcgcagtaga tggtggggag ccggtcggcc cggtaccagt ccctgaagct catcgggatg
      961 cctccagggc cttcgcgagc atccccggcg ggtggagggc cccgccaagc cgggggatgg
     1021 ggatgaccgg cacatcggtg tgcctctcga tctccctcgc cacctgcccg aggttcatct
     1081 ccgggacgag gaaggccttc gcctcgggga actccccgag ggcgaactcg gggaagggcc
     1141 agaccacccg gagccggaga tggccgatct cctccttcgg ccggtcgtgg aggagctgga
     1201 agacggaccg ggcaggggcc ccgtaggaga tgaagacctg ctcggcacct gggttcgtga
     1261 ccacgtagtc tgccatctcc ttcctcgcca actccacctt gtggacgagg cgcatgacga
     1321 gcttctcgtg gagccgcggg ttcgtggccg ccgggtagcc ccgctcgtcg tgggtgaggc
     1381 cggtgacgtg gacgtgctgg cccgtgccga actgcggaaa ggcggggaca aggtccgggt
     1441 ccgttgcccg gaagggcggg gtgccgggca cgaacggttt ccggccgacc cgctcgaccg
     1501 tgtccgggat gaggatcttc tcccgcatgt gcccgatcgt ctcgtccgcc atgagaaaga
     1561 ccggcacgcg gtaccggtcc gcgaggttaa agcacctcac ggtcagct
//
LOCUS       DATGDI010000127         3744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_99687, whole genome shotgun sequence.
ACCESSION   DATGDI010000127 DATGDI010000000
VERSION     DATGDI010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99687"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VKO45_04920"
     CDS             complement(<1..997)
                     /locus_tag="VKO45_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMA05250.1"
                     /translation="MTDRKFRLGTAALHAGQEPDPTTGSRAVPIYQTTSYVFRDTEHA
                     ANLFGLRELGNIYTRLMNPTTDVFEKRIAAIEGGTGAIATASGQAAITYALLNITRPG
                     DEIVSADNLYGGTYELFHYTFPKLGRNVIFVDSARPEAFRKAITPKTKAVYAETIGNP
                     KLDIPDFEAIAKIAHEAGIPFVVDNTTGIGLVRPIDHGADIIANSATKYIGGHGNSIG
                     GVIVDSGKFAWNNGKFPEFTEPDPSYHGLKYWDAFGNFPGLGNVAFVFKIRISLMRDT
                     GAVLSPFNAWLFLLGLETLHLRVPRHSENALAVAKFLMEQPKVAWVNYPGLPGHPS"
     gene            complement(1128..2492)
                     /gene="pscS"
                     /locus_tag="VKO45_04925"
     CDS             complement(1128..2492)
                     /gene="pscS"
                     /locus_tag="VKO45_04925"
                     /EC_number="2.5.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-phospho-L-seryl-tRNA:Cys-tRNA synthase"
                     /protein_id="HMA05251.1"
                     /translation="MNPRVQKIYEALFSLEEIRGIFRRSLPTGLDTVGETEFRERLAA
                     LRKIIDDLEAGTGTPQVTKIGGGLDIRCREEQFINIHPIQAAGRLTTEARKALISYGD
                     GYSTCDYCRKPFRLDKITKPGIADFHAELAKWLNMDQARVVPGARRGFQAVASSLVEK
                     GDPVIVSALAHYTEFLAVEEAGGVVREVPLNGQNIVTADATAQKIEEVQRETGRLPAL
                     VMIDHFDYQFANEHEIAGIGKVAHQYGIPFLYNGAYTVGVMPVDGKRIGADFVVGSGH
                     KSMASVAPSGVLAATGEYASKVLRTTAMVGDVTKRKFGIKEVEMMGCTLMGGTLLSMI
                     ASFPAVKERVVRWDDEVKKSNYFLGKLLQVTGSTVLSEYPRKHTLTKVDTTGSFDTVA
                     KTHKRRGFYLSDELSSRGIVGEFAGATRTWKLNTYGLTWDQIGYLGHAFTEIAEKHGL
                     EVAK"
     gene            3010..>3744
                     /locus_tag="VKO45_04930"
     CDS             3010..>3744
                     /locus_tag="VKO45_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA05252.1"
                     /translation="MKKKFVIGAAVVLVAIGIAYALGAFSTSSGGITGKGTVGILYAK
                     GVGPLPTLLATNQIDGYIAWQPFVEIAPLAGIGKVVSYSGALPPDDTWKNHPQNVFVA
                     RDDFVVANPDLVTALTALTVASDQYIADHPAEAAGTTADWLAGGGNFTYGNVSVSSVT
                     TMNRAITTIIYTTDPSAEWIAGTKKFIGAQADLGYVTGILKNASPDQQDAILFNFGPY
                     QNAKKELDTKQIVIPPVTATPIGFGYL"
BASE COUNT          662 a         1155 c         1125 g          802 t
ORIGIN      
        1 ggctcggatg gcccggaagg ccggggtaat tgacccaggc gaccttcggc tgttccatga
       61 ggaacttcgc cactgcgagg gcattctcgg agtggcgcgg tacccggagg tgcagggtct
      121 cgaggccgag aaggaagagc caggcattga agggggagag aaccgccccc gtgtctctca
      181 tgagggagat ccggatcttg aagacgaagg ccacgttccc gaggccaggg aagttcccga
      241 aggcatccca gtacttcaga ccatggtagc tcgggtcggg ctcggtgaac tccgggaact
      301 tcccgttgtt ccaggcaaac ttcccggaat cgacgatgac ccccccgatg gagttcccgt
      361 ggccgccgat gtatttcgtt gccgagttgg cgatgatgtc ggccccgtgg tcgatgggcc
      421 ggacgaggcc gatcccggtc gtgttgtcca cgacgaaggg gatgcccgcc tcgtgggcga
      481 tcttcgcgat ggcctcgaaa tcggggatgt cgagcttggg attcccgatg gtctctgcgt
      541 acacggcctt tgtcttcggg gtgatggcct tccggaaggc ctcgggtctc gccgaatcca
      601 cgaagatgac gttcctcccg agcttcggga aggtgtagtg gaagagctcg tacgtgccgc
      661 cgtacaggtt gtcggcagag acgatctcgt cacccggcct cgtgatgttc aggagggcgt
      721 aggtgatggc ggcctgcccg gacgccgtcg cgatcgcccc ggtcccgccc tcgatggcgg
      781 cgatgcgttt ctcgaagacg tctgtggtcg ggttcatgag ccgggtgtag atgttcccga
      841 gctccctgag gccgaagagg ttcgccgcat gctccgtgtc cctgaacacg taggacgttg
      901 tctggtagat cggtaccgcc cgggacccgg tcgtcgggtc gggctcctgc cctgcatgga
      961 gggcagcggt tcctaaacgg aatttcctgt cggtcatttt tcacactccc tgtggttttt
     1021 tcctgttcct gtcctattcc tgtcctgttc ttttcgtcgt ccgatcacga gggattccac
     1081 cctccgggtg acccccgcga tctctggaat tcacccgcca ggccgcatca tttcgcgacc
     1141 tccagcccat gcttctccgc gatctcggtg aaggcatggc cgaggtaccc gatctggtcc
     1201 cacgtgagcc cgtaggtgtt cagcttccag gtcctcgtcg cccccgcgaa ctcgccgacg
     1261 atgccgcggg acgagagctc gtcgcttaag taaaaccccc gtcgcttgtg ggtctttgcg
     1321 accgtgtcga agctccccgt ggtatcgacc ttggtgaggg tgtgcttcct cgggtactcc
     1381 gagaggaccg tgctcccggt gacctggagg agcttcccga ggaagtagtt ggacttcttc
     1441 acctcgtcgt cccagcggac cactcgctcc ttcacggccg ggaaggaggc gatcatggag
     1501 aggagggtcc cccccatcag ggtacacccc atcatctcca cttctttgat cccgaacttt
     1561 cgcttcgtta cgtcccccac catcgcagtg gttcggagga ccttcgatgc gtactccccg
     1621 gtagccgcga gcactccgga cggtgccacc gaggccatgc tcttgtgtcc ggagccgaca
     1681 acgaagtccg caccgatcct ctttccgtcg acgggcatca cgccgaccgt gtatgccccg
     1741 ttatagagga atgggattcc gtactggtgc gcgaccttcc cgatcccggc gatctcatgc
     1801 tcattcgcga actggtagtc gaagtggtcg atcatgacga gggcagggag cctcccggtc
     1861 tccctctgca cctcctcgat tttctgcgcc gtggcatccg cggtgacgat attctgcccg
     1921 ttcagtggga cctctctcac gaccccccca gcctcctcga cggcgaggaa ctcggtatag
     1981 tgggcgaggg ccgagacgat cacgggatct cccttctcca cgagggatga ggccaccgcc
     2041 tggaatcctc gccgggcacc gggcacgacc cgtgcctggt ccatgttcag ccatttcgca
     2101 agctcggcat ggaagtccgc gattcccggt ttcgtgatct tgtcgagccg gaagggtttg
     2161 cggcaatagt cacaggtgga gtacccgtct ccgtaggaaa tgagggcctt ccttgcctcc
     2221 gtggtgagcc tccccgcggc ctggatgggg tggatgttga tgaactgttc ctcccggcac
     2281 ctgatatcaa ggcccccgcc aatcttcgtg acctgcggag ttcccgtccc tgcctcaagg
     2341 tcgtcgataa tctttcggag ggcggcgagc cgctctctga actctgtctc cccgacggta
     2401 tcgaggcctg tcgggaggga cctccggaag atcccgcgga tctcctcgag gctgaaaagg
     2461 gcttcataga tcttttggac acgtgggttc atggacacct ccggaggcag ggtgaggtat
     2521 cctgccttca cgattgtgaa ctacttagag ataacaccgt gagaacttat aaagattcct
     2581 atagagcata ttttgcctat gtttcaatga gcggcatgca taaccacagg ttcaggatgc
     2641 caaggaatgg gatccccggg cgaatgattg agaacgtgag gtctccaccg gggcgatgcc
     2701 ccgttggttt tccgtcgggc tgatgccctc caggtgctcc accggggatc cctggagtcc
     2761 ttggagccgg ggcacaggtt tgccgcgctc gcgatggccg gtgtggaggc ctcgcgactc
     2821 ccttgtgacc cctgcagagt gttcacaatt gtgaactatt cttcatcctc tcgcagggcc
     2881 ttagagctgc cttcttaggc agatttttcc tgatcttcac ttgctaaatc ctgtccgggg
     2941 atgacagatc gacggcaatc tttgcctcaa taggaggccc tttcaaaatt caggtttcaa
     3001 gctctcttca tgaaaaagaa gtttgttata ggagcggcgg ttgtcctcgt cgccatcggg
     3061 atcgcgtatg ccctcggtgc attctccaca tcttccgggg gaataaccgg aaagggtacc
     3121 gtgggaatcc tgtatgcaaa aggagtcggg cctctcccca cgcttctcgc cacgaatcag
     3181 atcgacgggt acatcgcgtg gcagcccttc gtcgagatcg cccccctggc tgggataggg
     3241 aaggtcgtca gctactctgg ggcgcttccg cccgatgata cctggaagaa tcacccgcag
     3301 aatgtcttcg tggcgaggga cgatttcgtc gtggcgaacc ccgatctcgt gaccgcactc
     3361 accgccctca ccgtcgcctc cgaccaatac attgccgacc acccggccga ggccgccggc
     3421 accaccgccg actggctcgc agggggaggg aatttcacct acgggaatgt ctcagtatcc
     3481 tcggtgacca ccatgaaccg ggccatcacg accatcatct acaccacgga tcccagcgcg
     3541 gaatggatcg cggggacgaa gaagttcatc ggggcccagg ccgacctcgg gtatgtcacc
     3601 gggatcctga agaacgcgag cccggaccag caggacgcga tcctcttcaa cttcggcccg
     3661 taccagaatg cgaagaaaga gctcgatacg aagcagatcg tgatcccgcc cgtgaccgcg
     3721 acccccatcg ggttcgggta tctc
//
LOCUS       DATGDI010000128         4178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_186954, whole genome shotgun sequence.
ACCESSION   DATGDI010000128 DATGDI010000000
VERSION     DATGDI010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186954"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VKO45_04935"
     CDS             complement(<1..483)
                     /locus_tag="VKO45_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HMA05253.1"
                     /translation="MVLTKRIIPCLDLKDGRVVKGCHFIDLRDAGDPVALAQRYNEQG
                     ADEVVFLDITASRENRGIILDVIRRAADQLFLPLTVGGGIRSLEDIQQILRAGADKVS
                     INTSAVNDPALIGRSASRFGTQCTVVAMDVRRRLDDPAGKTPVPLPGGGTCWYEVVTY
                     G"
     gene            648..1391
                     /locus_tag="VKO45_04940"
     CDS             648..1391
                     /locus_tag="VKO45_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05254.1"
                     /translation="MDRTLSVVLAALVVASVIFLAVISLTAYIGITYRDTLSSTYEFR
                     VSITPDATLRNVTLYLPVPVRGSGPSAVLEGIGTGKLTGVPGGWNVSLIGTEKFTYLE
                     VWTREISPLPDARSYELAIDERVTGPIDTKNAGSGDLVLAPAAAMRQAACTGTDGGTS
                     PDLLCEQYESPVYADFTSPGNAHLRIFISLSGRNTWDVFGPSSNEYQDGLQVSFSEGA
                     QGWRPGSGMLVTGIGDYGLDPWLQGEGTG"
     gene            1388..2614
                     /locus_tag="VKO45_04945"
     CDS             1388..2614
                     /locus_tag="VKO45_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HMA05255.1"
                     /translation="MNTGRLKIEWARQYMPVLASIRREFEEGRPLRGLTVGMALHVEA
                     KTAVLVETLSAAGAEVCITGCNPLSTQDDVAAALNEVPHVHCYAKRGATVEEYYRAID
                     RVLEARPSITIDDGMDLIYALHTKRRDLLDGVIGGCEETTTGVHRLRAMAAEGALAFP
                     VLAVNDTPMKRYFDNVHGTGESALAAIMITTNVLMAGRNLVIAGYGFCGKGIARKAAG
                     LGARVIVTEVDPRVALQAHMDGYAVMTMEEAAPLGQIFITTTGNTGVITSRHFPRMRD
                     GAILANAGHFNVEVDRVWLEEHADSVVRRDGIDTYRFGDRSLHLLAEGRLVNLATPKG
                     MGHPVEVMDLSFALQALCTRHIAGKGRDLPAGVHEVPADIDEAVARRKLATLGLSIDR
                     LTDEQVKYQAGWSQGT"
     gene            complement(2565..3470)
                     /locus_tag="VKO45_04950"
     CDS             complement(2565..3470)
                     /locus_tag="VKO45_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HMA05256.1"
                     /translation="MGTIRLREDQPLDLALTLSCGQAFRWEERAGWWEGVVRGSVWRL
                     RQEADRVFYDGPTAPAVSRYLALDDDLPAILASVDRDPVIHRSLDACRGLRILRQDPW
                     EILVSFICATNTNIPRIRKMIASLAETFGHRVPGGGYPRFSFPSPRVLSGSCAHRLPG
                     CRLGYRERYVAETAARVAQDLSLLSRISGLPFPGARNSLLAFPGVGPKAADCILLFGF
                     HRLEAFPVDVWIRRIMHTHYLRPVPGLKVPSEKEDRRIRDFGREHFGMYAGYAQEYLF
                     CAREPLGDPVRSPGSSRPGTLPARR"
     gene            3595..>4178
                     /locus_tag="VKO45_04955"
     CDS             3595..>4178
                     /locus_tag="VKO45_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05257.1"
                     /translation="MTEIEEHYRTENGDILIEMRLRTVMQIFNSLDPSPFHEKDLDPD
                     AEAYFTEMVQDFPLSQPMRIVIHLPCKESECEEAKTLEPAIQNHFSYLELSAARERRF
                     KFRQGRVSLAIGIAFLLSMGIISTLISPYTQVGFAAWVAGGLLIVSWVAMWEPINTFL
                     YGWWPIRRKRQIYEKISRMKVEVRHDDHAGGEEVP"
BASE COUNT          752 a         1331 c         1415 g          680 t
ORIGIN      
        1 gccgtaggtc acgacctcgt accagcaggt cccccctccc gggagaggga cgggggtctt
       61 tcccgcaggg tcgtcgaggc ggcggcggac gtccatggcg acgaccgtgc actgggtccc
      121 gaaccgggag gcggagcggc cgatgagggc cgggtcattc accgcgctcg tgttgatgga
      181 gaccttgtca gccccggccc ggaggatctg ctggatgtcc tcgaggctgc ggatgccgcc
      241 ccccacggtg agggggagga agagctggtc ggcggcccgc cggatcacgt cgaggatgat
      301 gccccggttt tcccgggagg cggtgatgtc gaggaacacc acctcgtcgg ccccctgctc
      361 gttgtaccgc tgggcgagag cgacggggtc ccccgcgtcc cggagatcga tgaagtggca
      421 gcccttcacg acacggccgt ccttcaggtc gagacagggg atgatccgct tggtgagcac
      481 catggataag aatgggacac gaggtatatt ggttttggta tcccggggga cctgcgggcg
      541 gggagggatc ccacccctat aaggaaattc agggcggtta tcccggaacc cgtaagaacc
      601 cgcatgagtt catgtaggat gaggcgtaac ctaaccgtgg tgagaggatg gacagaaccc
      661 tttcggtcgt gctcgcggcc ctcgtggtgg cctcggtgat cttcctcgcg gtgatttccc
      721 tgaccgcgta catcgggatc acctaccggg acaccctctc gagcacctac gagttccggg
      781 tgagcatcac ccccgatgcg accctcagga acgtgaccct gtacctcccg gttccggtga
      841 ggggatccgg gccctcggcc gtcctcgagg ggatcgggac agggaagctc acgggggtgc
      901 ccggcgggtg gaacgtgtca ctgatcggga cggaaaagtt cacgtacctc gaggtctgga
      961 cccgcgagat ctcccccctt cccgacgccc gttcctatga gcttgccatc gacgaacggg
     1021 tgacggggcc tatcgatacg aagaatgcgg gttccgggga tctggtgctt gcccctgccg
     1081 cggcgatgag gcaggccgcg tgtaccggga cggatggcgg gacatcaccg gatctgctgt
     1141 gcgagcagta cgaaagcccg gtctacgccg acttcacctc ccccgggaat gcccacctcc
     1201 gtatcttcat ctctctctcc gggaggaaca cctgggatgt cttcgggccc tcctccaatg
     1261 aataccagga cggcctgcag gtctccttct cagagggcgc gcagggctgg cgcccgggga
     1321 gcggcatgct cgtcacgggt atcggggatt atggcctcga tccctggctg cagggggagg
     1381 ggacagggtg aacacgggaa ggctcaagat cgagtgggcc aggcagtaca tgcccgtcct
     1441 cgcctccatc cggcgggagt ttgaggaggg acgcccgctc cggggcctca ccgtcgggat
     1501 ggccctccac gtggaggcca agaccgcggt cctcgtggag accctctccg cggcgggggc
     1561 ggaggtctgc atcacgggct gcaaccccct ctccacccag gatgatgtgg cggcggccct
     1621 gaacgaggtg ccccatgtcc actgctatgc aaagaggggt gccacggtgg aggagtacta
     1681 ccgggccatc gaccgggtcc tcgaagcacg gccctctatt actatcgatg acgggatgga
     1741 cctcatctat gcccttcaca cgaaaaggcg ggacctcctc gacggggtta tcgggggctg
     1801 cgaggagacc accaccggcg tccaccggct acgggccatg gcagcggagg gggccctcgc
     1861 cttcccggtc ctcgcggtga acgacacgcc catgaagcgg tacttcgaca acgtccacgg
     1921 gaccggggag agcgccctcg ccgccatcat gatcacgacg aacgtcctta tggcaggaag
     1981 gaacctggtc atcgcgggct atggcttctg cgggaagggc atcgcccgga aggcagccgg
     2041 cctcggggcc cgggtcatcg tcaccgaggt ggatccgagg gtcgccctcc aggcccacat
     2101 ggacgggtat gccgtgatga ccatggagga ggcggccccg ctcgggcaga tcttcattac
     2161 cacgacgggg aacacgggag tgatcacctc gcgccacttc ccgcgcatgc gggacggcgc
     2221 catcctcgcg aacgcggggc acttcaatgt cgaggtggac cgggtatggc tcgaggagca
     2281 cgcggacagc gtggtccgga gagacgggat cgacacgtac cggttcgggg accggtccct
     2341 ccaccttctc gccgaaggaa ggctcgtgaa cctggccacc cccaagggga tggggcaccc
     2401 cgtcgaggtt atggacctct ccttcgccct ccaggcgctc tgcacccgcc acatcgccgg
     2461 taaggggagg gatctcccgg cgggggtcca tgaggtccct gccgacattg acgaggcggt
     2521 ggcccgccgg aagctcgcca ccctcggcct ttccatcgac cggctcaccg acgagcaggt
     2581 aaagtaccag gccggctgga gccaggggac ctgaccgggt ccccgagcgg ttcccgggcg
     2641 cagaagaggt actcctgggc ataccccgcg tacatcccga agtgctccct gccgaaatcc
     2701 cggattctcc ggtcctcctt ctccgacggt actttcaatc cgggcacggg cctgagataa
     2761 tgggtgtgca tgatcctccg gatccagacg tccacgggaa aggcctccag ccggtggaac
     2821 ccgaagagga ggatgcagtc cgcagccttc gggccgacgc cggggaaggc gagaagggag
     2881 ttcctcgctc cgggaaacgg aaggccggag atacggctca gaagggagag atcctgtgcc
     2941 actctcgcgg cggtttcggc gacgtaccgt tcgcggtagc cgagccggca cccgggcagg
     3001 cgatgggcgc aggacccgga aaggacccgg ggggaaggga agctgaaccg ggggtaacct
     3061 ccgcccggaa cccggtggcc gaaggtctcg gcgagggagg cgatcatctt ccgtatccgg
     3121 gggatgttcg tattggtcgc gcagatgaag gagacgagga tctcccacgg gtcctggcgg
     3181 aggatccgca gcccgcggca ggcatcgagg gaccggtgga tgacaggatc ccggtcgacc
     3241 gatgcgagga tggcggggag atcatcgtcg agagccaggt acctgctgac ggccggggcg
     3301 gtgggcccgt catagaaaac ccggtccgct tcctgccgca gccgccagac actccccctg
     3361 accaccccct cccaccagcc tgcccgctcc tcccaccgga aggcctgccc gcaggagagg
     3421 gtcagggcaa ggtcgagggg ctgatcctcc cggagccgga tcgtccccat gccctgaaga
     3481 atggaccctt ccggatgtaa taggatgcgg agggcatccg gtcaggcgtc ctcatggggc
     3541 ccggtgcgaa atcctcatgg gttttcggat tccacaggta gtgtcgggtt tcgcatgacc
     3601 gagatcgagg agcactaccg gaccgagaac ggcgacatcc tcatcgagat gaggctccgg
     3661 acggtcatgc agatcttcaa ttctctcgac ccctccccct tccacgagaa ggacctggac
     3721 cccgacgcgg aggcgtactt caccgagatg gtccaggact tccccctctc gcagcccatg
     3781 aggatcgtta tccacctccc ctgcaaggag tcggaatgcg aggaggcaaa gaccctcgag
     3841 cccgccatcc agaaccactt cagctacctc gagctctcgg ccgcccggga gcggcggttc
     3901 aagttccggc agggaagggt gagcctcgcc atcgggatag ccttcctcct ctccatgggg
     3961 atcatctcca ccctcatctc tccctatacc caggttggtt tcgccgcctg ggtcgccggg
     4021 gggctcctca tcgtgagctg ggtggccatg tgggagccca tcaacacctt cctgtatggc
     4081 tggtggccca tccggagaaa acggcagatc tacgagaaga tcagcaggat gaaggtggag
     4141 gtgcggcacg acgatcacgc aggcggggaa gaggttcc
//
LOCUS       DATGDI010000129         1896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_332439, whole genome shotgun sequence.
ACCESSION   DATGDI010000129 DATGDI010000000
VERSION     DATGDI010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332439"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /gene="mtrG"
                     /locus_tag="VKO45_04960"
     CDS             <1..351
                     /gene="mtrG"
                     /locus_tag="VKO45_04960"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit G"
                     /protein_id="HMA05258.1"
                     /translation="FQEQVKIVDIMETEDNAAIKAKIAELKAQDPGAFPADAMVVQVK
                     EAGGAPEVAAAGVNPQFLEIEKRLDALEKKIEFTDAEVAQRVGRKIGRDIGILYGLVA
                     GLVIFYLLNILRFI"
     gene            373..1413
                     /gene="mtrH"
                     /locus_tag="VKO45_04965"
     CDS             373..1413
                     /gene="mtrH"
                     /locus_tag="VKO45_04965"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit H"
                     /protein_id="HMA05259.1"
                     /translation="MFKFEKEQQVWDFNGTKLGGQPGEYPTVLAASIFYNKHEIVLDD
                     KTGKIDKPKAEALWARCQELSDTVNIPHFIQILAESGPAMEKYIEWFDSIDHKTAFLM
                     DSSVPAVLAGACKYVTDVGLAHRAIYNSINGSIGAANIEALKNSDVDSAIVLAFNPAD
                     PSVFGRQKVLAEGGVAGQAKGMLQIAEECGIKRPILDTAATPLGLGSGSSYREILACK
                     AIFGYPTGGAYHNMTVAWTWLRRWKGTSKTPSLLAKGYEGKPALLEQMAHHYLGGMEG
                     IKQAAWSAPDIGCNLIASTLGANLIMYGPIENCEPMITSQAYMDIVTLEAVRSLGVDV
                     KEGVKHPIQKLI"
BASE COUNT          412 a          575 c          565 g          344 t
ORIGIN      
        1 ttccaggagc aggtcaagat cgtcgacatc atggagaccg aggacaacgc cgccatcaag
       61 gccaagatcg ctgaactcaa ggcccaggac cccggtgcct tcccggccga cgctatggtc
      121 gtccaggtca aggaggccgg cggtgcacct gaggtcgcgg cagcaggagt gaacccgcag
      181 ttcctcgaga tcgagaagag actcgatgcc ctcgagaaga agatcgagtt caccgatgca
      241 gaggtcgcac agcgcgttgg caggaagatc ggtcgagaca ttgggatact ctatggactg
      301 gtagcaggac tggtgatatt ctatctcctg aacatcctgc ggttcatctg agcgtacgga
      361 ggtgaaagaa aaatgttcaa attcgagaaa gaacagcagg tctgggactt caacggaacc
      421 aagctcggtg gacagcccgg cgagtacccg acggtgctcg cggcctccat cttctacaac
      481 aagcatgaga tcgtactgga tgacaagacg gggaagatcg acaagccgaa ggccgaggcc
      541 ctctgggcac gctgccagga gctctctgac acggtgaaca tcccccactt catccagatc
      601 ctggcagagt ccgggccggc aatggagaag tacatcgaat ggttcgacag catcgaccac
      661 aagacggcct tcctcatgga ctcctcggtg cccgccgtgc tcgcgggtgc gtgcaagtac
      721 gtcaccgacg tgggccttgc ccaccgggcg atctacaact ccatcaacgg gtccatcggg
      781 gccgcaaaca tcgaggcatt gaagaacagc gacgtggact cggccatcgt gcttgccttc
      841 aacccggccg acccctcggt cttcgggaga cagaaggtgc tcgccgaggg cggggtcgcg
      901 ggacaggcga agggaatgct ccagatcgcc gaggagtgcg ggatcaagcg cccgatcctc
      961 gacacggcgg caaccccgct gggtctcgga tccgggagtt cctaccgtga gatcctggca
     1021 tgcaaggcca tcttcgggta tcccacgggc ggtgcctacc acaacatgac ggtggcctgg
     1081 acatggcttc gccgctggaa gggaacgtcg aagaccccct cgctcctcgc caaggggtac
     1141 gagggtaagc cggccctcct cgagcagatg gcgcaccact acctcggggg catggagggt
     1201 atcaagcagg cggcatggtc tgctcccgat atcgggtgca accttatcgc gagcaccctc
     1261 ggtgccaacc tgatcatgta cgggcccatc gagaactgtg aacccatgat cacctcgcag
     1321 gcgtacatgg acatcgtgac cctcgaggcg gtccgttccc tgggcgtcga cgtgaaggag
     1381 ggtgtgaagc accccatcca gaaactcatt taaacttttt tttcccggga ttttctcagc
     1441 gagcggaagc caatattcaa tcgccaattg gctgctctcc gggattatgc ctgtcaccat
     1501 gaccgtcctg tattactccc ctctgcaact ataagccggt ggacagctta ggtgacacgt
     1561 ggacgctctc gggggctccg cccccgccgc tttggcaccc cccggtgcga tgggctgatc
     1621 cacagttacc ggaaaaaccg gggaactctc cggcgaatac cgtcaaagga tgacctgcct
     1681 atcccaaccc gggggacgca gaagcggcgg gggcgagcga cgcgagtccc cctggagcgt
     1741 ccaccttgta gccatacagt cgaccggctt atagtcggga agggatccgg aaaactgtgg
     1801 gtcgatggta aacctaccca tagagttttt tcatcaccca ggccatgttc tgtcccagga
     1861 cccgcatcgt cctgagccct tcttcgtctt tctcta
//
LOCUS       DATGDI010000130         4107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_129081, whole genome shotgun sequence.
ACCESSION   DATGDI010000130 DATGDI010000000
VERSION     DATGDI010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129081"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..407
                     /locus_tag="VKO45_04970"
     CDS             18..407
                     /locus_tag="VKO45_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMA05260.1"
                     /translation="MPGRWRRCETLPELLREADILTIHAAPPLNGRPIIMKRQLHICK
                     KGVILINTARGSLLSEGDLVEALREGRVAGAGLDVFSSEPYQGPLLEFPQVIMTPHVA
                     SNTVESRAEMEMEAVQNLISALRETEA"
     gene            404..1342
                     /locus_tag="VKO45_04975"
     CDS             404..1342
                     /locus_tag="VKO45_04975"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMA05261.1"
                     /translation="MKALVTGGSGFLGHYLVKELLKRDIETVVLDTVDPDSFFLEGCT
                     PPGGIRCRYIQGDIMDPVVLENALKGCDLMFHTAAIADLDEARRTPIRTMEVNVMGTA
                     RCLEAARKEGVSRFLFASSIYTAGRRGSFYRASKQAGESLCKIFEEEFGLPYTILRYG
                     SLYGRESNQWNFIYGVCRALLTTGEFTYVSSPDSEREYIHIADAARETVRIALDPGFV
                     NKAVLITGHQRMKVRELFAMIQEILGREITIHYVPQQQRTHYVMTPYSFESDIPVRIN
                     LASYVDISEGILDCLHEVQKELGRETGSIQESSPGP"
     gene            1398..2276
                     /locus_tag="VKO45_04980"
     CDS             1398..2276
                     /locus_tag="VKO45_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMA05262.1"
                     /translation="MMQCYLCGNREFTLITERLRYEEPRAVYRCNRCGLVFLHPTMTP
                     EEERAFYEKEYGEIFSKEKGTTPENLFEARSGDARMYRDWVSPFLDREDDCLEIGCAS
                     GYFLMAIREQVRSIHGMEPHAVLNRFCREMGISMIPDLAACRDGRFTKIFMFFLLEHL
                     GDPAGLLAEAGRVLRPGGKLFIVVPNVEDALLSLYEIPAFRDFYYTPAHQFYYSKKTL
                     SDLLVKSGFSRFGILPRQRYDLSNHMHWMQYGKPGGTGRYNAVFSEELRGEYAKCLTD
                     HFLCDTLFAVAEKGEG"
     gene            2278..3330
                     /locus_tag="VKO45_04985"
     CDS             2278..3330
                     /locus_tag="VKO45_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMA05263.1"
                     /translation="MAAGKPGRNEGAIRAAIIGFGGMGQRHHQAYLQNGIEVAAVADW
                     DTEKIRKLLPHLPSRHIYRDDEELLDGEEVDILSVVTNGPTHAGIAIRAAEHGIPMVL
                     CEKPVATSLADARRVIEAAERHGTRLAVNHIRRWSPTYRRLRSLLGEGVIGEIRHLSF
                     SMGSTGIGNFPLHFFDTARFLTGSEPGWAVGFLDSTGTPNPRGKQFIDPGGYGIFGFR
                     NGARFFFDSSEDTGVQYLFEIVGSYGRILIDELNDVWQIRARPEADRTIPFTRYGTGM
                     PLVPYQRDAPHDIVKLTAAAIAELAGGGPVSCTGDDGLRALEMVIALHVSDEQGHAQV
                     SLPLGPEHAAREVRIA"
     gene            3323..>4107
                     /locus_tag="VKO45_04990"
     CDS             3323..>4107
                     /locus_tag="VKO45_04990"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMA05264.1"
                     /translation="MPERSRGEAAGRKRHLLVTGGAGFIGSHLARRCLSLGHEVTVID
                     NLATGQRGNVPPGAAFIEGDISDEATLDRIPPGRIDAVLHLAAQSSGEISDERPDLDL
                     RVNTLGTLLLLSFCRRRGIPRFLYASSMAVYGDPPRNPVSEEVPCHPLSFYGISKRAS
                     ELYIDHFGRAGLATTCFRMFSVYGPGQDLSNLKQGMVSIFLAYLLEGKPIHVKGSGDR
                     FRDFVYIDDVVEAWIRALDQPATYGKVYNLATGVRTLVRELVR"
BASE COUNT          771 a         1286 c         1313 g          737 t
ORIGIN      
        1 ccgcggaccc gccgggtgtg ccaggccggt ggagaaggtg cgagacactg ccggaactcc
       61 tcagggaggc cgatattctt acgattcacg cagctccgcc attgaatggc aggccaatta
      121 ttatgaaaag acaacttcat atctgtaaaa agggggtcat tctcatcaac accgcccgtg
      181 ggagtctgtt aagtgaaggg gacctggtcg aggctctccg ggaggggcgg gtggcgggtg
      241 ccgggcttga tgtgttctca tccgagccgt accaaggtcc cctcctggaa ttcccccagg
      301 tgatcatgac cccccatgtg gcatcgaaca ccgttgaatc gagggcagag atggagatgg
      361 aggctgtgca gaacctgatt tcagctctcc gggagacgga ggcatgaagg cgctcgtcac
      421 cggcgggtcc ggcttcctcg ggcattacct ggtgaaggag ctcctgaagc gggacatcga
      481 gaccgttgtc ctggacacgg tggaccccga tagcttcttc ctcgaggggt gcaccccgcc
      541 gggaggcata cggtgcaggt acatccaggg ggatatcatg gacccggtcg tccttgagaa
      601 tgccctcaag ggctgcgacc tgatgtttca cacggcggcc atcgccgacc tggatgaggc
      661 ccgccgcacg cccatccgga ccatggaggt gaacgtgatg ggcaccgccc ggtgcctgga
      721 ggcggcccgg aaggaggggg tctcccgctt cctgttcgcg agctccatct acaccgccgg
      781 aaggagagga tccttttacc gggcctccaa gcaggccggg gaatccctgt gcaagatctt
      841 cgaggaggag ttcggacttc cctacaccat cctgcgttac gggagcctgt acgggcgcga
      901 gtccaaccag tggaacttca tctacggggt ctgccgggcc ctgctcacca cgggcgagtt
      961 cacctatgtc tcgagccccg actccgagcg ggagtacatc cacatcgccg atgccgcccg
     1021 ggagacggtg cgcatcgccc tcgaccccgg cttcgtgaac aaggcggtcc tcatcacggg
     1081 tcatcagagg atgaaggtgc gggagctctt cgccatgatc caggagatcc tgggaaggga
     1141 gatcaccatc cattacgtgc cccagcagca gcggacccac tatgtcatga ccccctattc
     1201 ctttgaatcg gatattccgg tccgaatcaa cctcgcctcc tatgtcgata tcagcgaggg
     1261 gatcctggat tgcctgcacg aggtgcagaa ggagctcgga agggagaccg ggtccatcca
     1321 ggaatcctct cccggaccat gatgcacgcg tgcctctgct tcaggagcgg ttccggactg
     1381 gggggtggga agcgggcatg atgcaatgct acctctgcgg taaccgggag ttcaccctca
     1441 tcaccgaaag actcaggtac gaagagcccc gggcggtcta ccggtgcaac cggtgcgggc
     1501 tcgtcttcct ccacccgacc atgacgccgg aggaggagag ggccttctac gagaaggagt
     1561 acggcgagat cttcagtaag gaaaaaggta ccactcccga gaatctcttt gaggcccgat
     1621 cgggcgacgc caggatgtac cgcgactggg tgagcccctt cctcgaccgg gaggatgact
     1681 gcctcgagat cgggtgcgcc tccgggtact tcctcatggc catccgcgaa caggtccgaa
     1741 gcatccatgg catggaaccc cacgcggtcc tgaaccggtt ctgccgggag atggggatat
     1801 ccatgatccc cgacctcgcg gcctgccggg atggcaggtt cacgaagatc ttcatgtttt
     1861 tccttctcga gcacctcggg gatcccgccg gtctcctcgc ggaggcgggg agggtcctcc
     1921 ggccgggggg aaaactcttc atcgtggttc ccaatgtgga ggatgccctc ctctccctgt
     1981 acgagatccc ggccttcagg gacttctact acaccccggc gcaccagttc tactactcga
     2041 aaaagaccct ttccgacctc ctcgtgaaaa gcgggttttc caggttcggg atactcccca
     2101 ggcagcggta tgacctctcc aaccatatgc actggatgca gtacgggaag ccggggggca
     2161 cgggccggta caacgcggtc ttctcggaag aactgcgggg tgagtacgcg aagtgcctga
     2221 cggatcattt cctctgcgat accctctttg ccgtcgccga aaagggggag gggtagcatg
     2281 gccgcgggaa agcccgggag gaatgaggga gcgatccggg ccgccatcat cgggttcgga
     2341 gggatgggac agcggcacca ccaggcctat cttcagaatg gcatcgaggt cgccgcggtc
     2401 gcagactggg atacggagaa gatccggaag ctgctcccgc atctcccgtc gaggcacatc
     2461 taccgggatg acgaggagct cctcgatggc gaggaggtgg acatcctctc ggtggtgacg
     2521 aacggaccca cccacgccgg gatcgccatc cgcgcagcgg agcatgggat cccgatggtc
     2581 ctctgcgaaa agcctgtcgc gacaagcctc gcggacgccc ggcgggtcat cgaagcggcc
     2641 gaaaggcatg gaacgagact cgccgtcaac catatcagac ggtggtcccc tacctaccgc
     2701 cggctccgat ccctccttgg agagggggtc atcggggaga tccggcacct ctccttctcc
     2761 atgggttcga cgggcatcgg gaactttccc ctgcacttct ttgataccgc ccggttcctc
     2821 accgggagcg agcccgggtg ggccgtgggg tttctggata gcaccgggac cccgaacccc
     2881 cgcggaaagc agttcatcga tcccggcggg tacggcattt tcgggttccg gaacggcgcg
     2941 aggttcttct tcgattcctc cgaggacacc ggcgtccagt acctcttcga gatcgttggt
     3001 tcctacggga ggatcctcat cgacgagctg aacgacgtct ggcagatccg ggcccggccg
     3061 gaggcggacc gaaccatccc cttcaccagg tacggtaccg gtatgcccct tgtgccctat
     3121 cagcgggacg ctccccatga tattgtgaag ctcacggcgg ccgccatcgc cgagctcgcc
     3181 ggaggaggac cggtgagctg cacgggggac gacggactcc gggccctcga gatggtcatc
     3241 gccctccatg tctcggacga gcagggccat gcacaggttt ccttacccct cggacccgag
     3301 catgcggcga gggaggtccg gattgcctga gaggagccgc ggggaggcgg caggcaggaa
     3361 acgacacctc ctcgtcacgg ggggagcggg gttcatcggg tctcacctcg cccggcggtg
     3421 tctctccctg ggccacgagg tgaccgtcat cgacaacctt gccacgggac agaggggaaa
     3481 cgtgcctccc ggggcggcct tcatcgaggg ggacatctcg gacgaggcca ccctcgaccg
     3541 catccccccg gggaggatcg atgcggtcct ccacctcgcg gcccagtcct cgggggagat
     3601 ctccgatgag cgccccgacc ttgacctcag ggtgaacacc ctcggaactc tcctcctcct
     3661 ctccttctgc aggagaagag ggatcccgag gttcctctac gcgagctcca tggcggtata
     3721 cggggacccg ccccggaacc ccgtctccga ggaggtcccg tgccatcccc tctccttcta
     3781 cgggatctcg aagcgggcga gcgagctcta catcgatcac ttcgggaggg caggccttgc
     3841 caccacctgc ttccggatgt tctcggtcta cggcccgggg caggacctct cgaacctgaa
     3901 gcaggggatg gtgagtatct tcctcgcata cctcctcgag ggaaagccca tccacgtgaa
     3961 ggggagcggg gatcgtttcc gggacttcgt ctatatcgac gacgtggtgg aggcatggat
     4021 acgggcactg gaccagcccg ccacttacgg gaaagtctac aaccttgcca cgggggtccg
     4081 gaccctggtg agggagctgg tccgtga
//
LOCUS       DATGDI010000131         1732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_216499, whole genome shotgun sequence.
ACCESSION   DATGDI010000131 DATGDI010000000
VERSION     DATGDI010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216499"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="VKO45_04995"
     CDS             complement(<1..162)
                     /locus_tag="VKO45_04995"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107117.1"
                     /note="catalyzes the reversible formation of fructose
                     1,6-bisphosphate from glycerone phosphate and
                     D-glyceraldehyde 3-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HMA05265.1"
                     /translation="MIGKEIRIERIINRNTRRAVIIPMDHGFSLGQIEGLTNMTNVIT
                     DVAKGGANAI"
     gene            complement(159..971)
                     /locus_tag="VKO45_05000"
     CDS             complement(159..971)
                     /locus_tag="VKO45_05000"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HMA05266.1"
                     /translation="MRGKEIRLERIMDRNTGKTIIVPMDHGVTNGPMKGLTDMGETVG
                     LVAEGGANAVLGHLGLALYGHRRHGRDVGLILHLSGSTSLGPDPNEKVLVNTVENAMK
                     MGADGVSIHVNIGAGSEARMLSDLGKVAIECMEWGMPLLAMMYPRGAKIRDEHGVESV
                     ALAARVAAELGADLVKTVYTGDPESFREVTRGCPVPVVVAGGSKTDERATLELVAGAM
                     EGGAAGISIGRNAFQHPAPDRFVRAAAAIVHERRSVEEALEIMKDEGRRRKR"
     gene            complement(1303..>1732)
                     /locus_tag="VKO45_05005"
     CDS             complement(1303..>1732)
                     /locus_tag="VKO45_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMA05267.1"
                     /translation="RLGYDILAGCLGGGFMTWAKAAEPVGTVRTWTVHELKANLGDPN
                     LYLLDLRDTPNYRKHGHLPGAHHVYVGELPSHICKVPSPEREIVCFCDAGYKSSIAAS
                     LLLQQGFTKVTVVLGSMAAWEKAGYPVEKEQEPEGPAEQG"
BASE COUNT          277 a          614 c          513 g          328 t
ORIGIN      
        1 gatggcgttt gccccgccct tcgcgacgtc ggtgatgacg ttcgtcatgt tcgtgagccc
       61 ctcgatctgc cccagggaga acccatggtc catcggaatg atgacggccc tcctcgtgtt
      121 ccggttgatg atccgctcga tccggatctc ctttccgatc atcgctttct cctccttccc
      181 tcatctttca tgatttccag tgcttcttca acgcttcgcc tctcgtgcac gatggccgcc
      241 gccgcccgga cgaaccgatc cggggcaggg tgctggaagg cgttccggcc gatggagatg
      301 ccggccgcgc cgccctccat ggcccccgcc acgagctcga gagttgcccg ctcgtcggtc
      361 ttggaaccgc cggccaccac caccgggacc gggcagcccc gggtcacctc ccggaaactc
      421 tccgggtctc ccgtgtacac ggtcttcacg aggtcagccc cgagctcggc cgccacccgg
      481 gcggcgaggg ccacggactc caccccgtgc tcgtccctga tctttgcgcc cctcgggtac
      541 atcatggcga ggaggggcat cccccactcc atgcactcga tggcgacctt cccgaggtcc
      601 gagagcatcc tcgcctcgga cccggcgccg atgttcacgt ggatggagac accgtcggcc
      661 cccatcttca tggcattctc caccgtgttc acgagcacct tctcgttcgg gtcagggcca
      721 agggacgtcg agcccgagag gtggaggatg agccccacgt cgcgcccgtg cctccggtgg
      781 ccgtagaggg cgaggccgag gtgcccgagc accgcgttcg cccccccttc cgcgacgaga
      841 cccacggtct cgcccatgtc tgtcaggccc ttcatggggc cgttcgtcac cccgtggtcc
      901 atggggacga tgatggtctt ccccgtgttc cggtccatga ttcgttccag acggatctct
      961 tttcctctca tgtacacctc ccccgccggg aacaccggtc aggggaaacc atgagggccc
     1021 gagaaccgtt ccggcggcgg gaaaattacc cgaaatagcc gaagaagcga taatagccga
     1081 aaaaggcccc tgctcctcgg gtcatggtca gagaacagtt cactgcgggc aggacggagg
     1141 cttcactcgg ggcgataaag gcaatcccgg gcgaaaaggc atccaccacg cattggctaa
     1201 gctatagcac ggccggagta tttaaaatga tcagatcctc ccgggggctc cgcccccagg
     1261 aacgtccgtg atgtcaccgt gcgcgtattt cgcttgactt ccctatccct gctccgccgg
     1321 tccctccggt tcctgctcct tctccaccgg gtaccccgcc ttctcccatg ccgccatgga
     1381 cccgaggacg acggtgacct tcgtgaaccc ctgctgcagg agcaggctcg ccgcgatgga
     1441 ggacttgtac cccgcgtcgc agaagcagac gatctccctc tccggggacg gcaccttgca
     1501 gatgtgcgac gggagctccc cgacatagac gtggtgggcg cccggaagat gcccgtgctt
     1561 ccggtagttc ggtgtgtccc tgaggtcgag gagatagagg ttcgggtccc cgaggttcgc
     1621 cttcagctcg tggacggtcc aggtccgcac ggtgcccacg ggctccgctg ccttcgccca
     1681 ggtcatgaac cctcctccga ggcagcccgc aaggatgtcg tacccgagcc gg
//
LOCUS       DATGDI010000132         5794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_12869, whole genome shotgun sequence.
ACCESSION   DATGDI010000132 DATGDI010000000
VERSION     DATGDI010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12869"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..364
                     /locus_tag="VKO45_05010"
     CDS             134..364
                     /locus_tag="VKO45_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05268.1"
                     /translation="MTEQHEREIRCPRCGWWWHHPREPVHFASWDEWGEEHPEKENLT
                     CPNCGEVTEVSEGNVRFSKLGKGKKGSGTRKR"
     gene            complement(370..2835)
                     /locus_tag="VKO45_05015"
     CDS             complement(370..2835)
                     /locus_tag="VKO45_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HMA05269.1"
                     /translation="MDPGTLADGRTGLSEGEARKRLTQYGPNRLSKPWEITLLGIARE
                     EITEPMILLLLAVGIFYTLLASFGDALTLYAIIATLVAVEIANEYRAKKAISSLAALA
                     EPRTKAVRDGKITEVATEEVVPGDLLVLAAGTRVSADGKILSGVSLQADESSLTGESF
                     PAEKNPGDDLFAGTLVVSGEGILEVTLTGGGTRIGRISELARVIRPPKTPLQIAMKSL
                     AKTLVFVALFFSIAIPAIGVLQGQDLRVMFLTALALAFATIPEELPIIITMNLGLGSW
                     LLSRENFLVKKLKAAETLGNATVILTDKTGTLTESAMRVVEIFPPQDAGRVLSAARAA
                     MTDLSLSITDRALVEAAAARSLPVPAGRVVRERAFDRGRNTRAIIRENGGKATLFLIG
                     APEEVLAGAAGDNRGAVEALAAEAAKGRRVIGVAHRELDGAEAGRPFGELERGLTLDG
                     LVSLEDPPRPGVRETIGRAQRAGIRTVMVTGDHPKTAAAIAAEVGIPPAPLLEGKELD
                     ALSDADLRGRVKEVSVYARATPEHKYRLVKALQENGEVVAVTGDGVNDSLALKGADIG
                     IAMGIRGTDAAKEAADIVIADDNFITIGKGVFEGRRIFDNLSKGVRYYLSVKIALIAV
                     FAASLALSIPFPFSPIQIILLELFMDLGASASFVAEPAEPSIDRRPPRDPRVPFLGGG
                     MVTGIAAGGIALFVAVFATYGFALRAAPHQAQTYAFSAWMIGHVLLALFSRSAHDPMY
                     RIGPFSNRVLLLWMAGALGFLAAAILIPPLGQHLNVVPVTGAGIVSVAIFVFLCMAGF
                     EVAKIVRAARNPADPEKKEESAA"
     gene            2918..3793
                     /locus_tag="VKO45_05020"
     CDS             2918..3793
                     /locus_tag="VKO45_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HMA05270.1"
                     /translation="MRGVPIAESFGIQLFISSIPYMVWMDASRWILPDLASALAWARE
                     RNGQGIRCTLALAGEFARTPGQAGSAADGNLRCIAGIAVAGLDASLSVKPSALGGLFD
                     PEACRGHATGLAREAARSGVPLELDMEGRNSVDSTLDIAAGCRRENPRVMVALQAYLD
                     RTPGDIRNMVSLGIGVRLVKGAYLGDVSDYRQVRGRMKECAGILQDLGASFSLGTHDP
                     VLIGWAMEEFADGKDLVEFGFLKGLSDETKIRLASGGWRVSEYVPYGRGEEGYVTRRE
                     RYLANLAGAGLAPAP"
     gene            complement(3823..4125)
                     /locus_tag="VKO45_05025"
     CDS             complement(3823..4125)
                     /locus_tag="VKO45_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05271.1"
                     /translation="MAADKGEGSGSEGRHRRTGIRESLLDRYRVILVNKLTIKDLSEL
                     KDYLRKEFDEEGNLNREVREKFEFKLRQFLDEEVLTREIVGIEALIRSLRPGDLKR"
     gene            4259..4642
                     /locus_tag="VKO45_05030"
     CDS             4259..4642
                     /locus_tag="VKO45_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HMA05272.1"
                     /translation="MADTKNFGELIYTFDKAAGEALGKRESHTPKIEAPATVKPDQAF
                     DLKVMVGPHPNTIEHSIRWITVWVNEDGRAFNPIFIGKASLTPVVAQPEVVFKVKLQK
                     GGVIHAVEYCNLHGLWSGKKEIKVG"
     gene            4754..>5794
                     /locus_tag="VKO45_05035"
     CDS             4754..>5794
                     /locus_tag="VKO45_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05273.1"
                     /translation="MTDTNGEQARAIMLDFARLTGLDPEGRGPGRYLWTDAFAVCNYV
                     GLWQATGDEGLRDLGLRLVDQVHHTLGRHRGDDPRTGWLSGLGEEDGESHPTIGGLRI
                     GKKLPERALGEPPDERLEWDQDGQYFHYLTKWMHALVIAGRATGDPVFLRWAAELART
                     AYAGFIHRPRSGGPDRMYWKMSIDLSRPLVASMGQHDPLDGLVTFCEIQVARGTYPAP
                     FLPPDLGEEIAGLAGISQGMDLPTDDPLGTGGLISDALRIAQVMVQGGGRFPEQAVPE
                     LDLPALLGEVLNAALLGLDAFADSGCTGLPADFRLAFRELGLSIGLSGAGELIRLIDG
                     HPSRFGSGSSLQV"
BASE COUNT          969 a         1877 c         1963 g          985 t
ORIGIN      
        1 gcccagccct cggtgcccga gggcctccgc cccctcgtcc gggagctctc gaggcgcctg
       61 aagagctgag cgggagccgg tcgcgattcc cgtcccgcaa gagttatttc ctccccgtcc
      121 gacatcaggc gcgatgacag aacagcatga acgcgagatc cggtgcccga ggtgcgggtg
      181 gtggtggcac cacccgaggg aacccgtcca ttttgcctcc tgggacgagt ggggcgagga
      241 gcaccccgag aaggagaacc tcacctgccc gaactgcgga gaggtgaccg aggtctctga
      301 ggggaacgtc cggttttcga agctcggaaa agggaagaag ggatccggga cccggaagag
      361 gtaggcccct caggccgcgg attcctcctt cttttccgga tcagccgggt tccgtgcggc
      421 tctcacgatc tttgccactt cgaacccggc catgcagagg aacacgaaga tcgccacgga
      481 tacgatcccc gccccggtga ccgggacgac gttcaggtgc tgccccaggg gcgggatgag
      541 gatcgcggcc gcgaggaacc cgagcgctcc tgccatccag agaagaagca cccggttcga
      601 gaagggcccg atccggtaca tgggatcgtg cgccgatcgg gagaagaggg caaggaggac
      661 gtggccgatc atccaggccg agaaggcata ggtctgggcc tggtgcgggg ccgcccggag
      721 agcgaacccg taggtcgcaa agaccgcgac aaagagggcg atcccgccag cagcgatgcc
      781 cgtgaccatg ccccctccga ggaagggaac ccgggggtca cggggcgggc gccggtcgat
      841 ggagggctcg gccggctcgg ccacgaagga ggccgaggcg ccgaggtcca tgaagagctc
      901 gaggaggatg atctggatcg gggaaaaggg gaaggggatg gagagggcga gggatgcggc
      961 aaagacggcg atgagggcga tcttcaccga gaggtagtac cggaccccct tggagaggtt
     1021 gtcgaagatc cgccggccct cgaagacccc cttcccgatg gtgatgaagt tgtcatcggc
     1081 gatcacgatg tcggccgcct ccttcgccgc atcggtcccc cggatgccca tggcgatgcc
     1141 gatgtccgcc cccttcaggg cgagggagtc gttcaccccg tccccggtga ccgccaccac
     1201 ctccccgttt tcctggaggg ccttcacgag ccggtacttg tgctccggcg ttgcccgggc
     1261 gtacaccgag acctccttca ccctcccgcg gagatccgcg tccgaaaggg cgtcgagctc
     1321 ctttccctcg aggaggggag ccggcgggat gcccacctcg gcggcgatgg ccgccgcggt
     1381 cttcgggtgg tcacccgtga ccatgaccgt ccggatgccc gcccgttggg cccgcccgat
     1441 ggtctccctc accccgggcc tcggcgggtc ctccagggag acgaggccgt cgagggtgag
     1501 accccgctcc agctccccga agggccggcc ggcctcggcg ccgtcaagtt cccggtgggc
     1561 gaccccgatg acccgcctgc ccttcgccgc ctcggccgcg agggcctcca ccgccccgcg
     1621 gttgtcaccg gccgcccccg cgaggacctc ctcgggggcg ccgatgagga agagggtcgc
     1681 cttcccgccg ttctcccgga tgatcgcccg ggtattcctc ccccggtcga aggcccgctc
     1741 ccggacgacc ctgccggcgg gtaccggtag agatcgggcc gcggccgctt ccacgagcgc
     1801 cctgtcggtg atggagaggg agagatcggt catggcagcc ctcgccgcag agaggaccct
     1861 tcccgcatcc tgcgggggaa agatctccac gacccgcatc gcgctctcgg tgagggtccc
     1921 cgtcttgtcg gtgaggatca ccgtggcatt ccccagggtc tcggcggcct tcagcttctt
     1981 cacgaggaag ttctccctcg aaagaagcca ggagccgagg cccagattca tggtgatgat
     2041 gatggggagc tcctcgggga tggtggcaaa ggcgagggcg agggccgtga ggaacatcac
     2101 ccggagatcc tggccctgga ggacgccgat ggcggggatg gcgatggaga agaagagggc
     2161 gacaaagacg agggtctttg caagggactt catggcgatc tggagggggg ttttcggggg
     2221 ccggatcacc cgggcgagct ccgagatcct cccgatgcgc gtgcccccgc ccgtgagggt
     2281 gacctcgagg atcccctcgc cggacacgac cagggtcccg gcgaagagat cgtcgcccgg
     2341 gttcttctcc gccgggaagg actcgccggt cagggacgac tcgtctgcct ggaggctcac
     2401 gcccgagagg atcttcccgt cggcgctgac cctcgttccg gcggcgagga cgaggaggtc
     2461 gcccggcacc acctcctcgg tggcaacctc ggtgatcttc ccgtcccgca cggccttcgt
     2521 cctcggctcg gcgagggccg cgagggagga gatggccttc ttggcccggt actcgttcgc
     2581 gatctccacc gcgaccaggg tggcgatgat ggcgtagagg gtgagggcgt ccccgaaact
     2641 cgcgaggagg gtgtagaaaa tcccgacggc gaggaggagg aggatcatcg gctcggtgat
     2701 ttcctcccgc gcgatgccga ggagggtgat ctcccagggt ttcgacagcc ggttgggccc
     2761 gtactgagta agcctcttcc gggcctctcc ctcggaaagg cccgtccgtc cgtccgcgag
     2821 cgtaccggga tccataccag tgtgtccgcg gttatataat agtggattcc cattttcctt
     2881 ttcccgatac accggaccgg cggcggtgcc cggtcggttg agaggagtac ccatcgcgga
     2941 atccttcggg atccagcttt ttatcagctc catcccctac atggtgtgga tggatgcctc
     3001 gcgctggatc ctgcctgacc tggcatcggc ccttgcctgg gcgcgggaga ggaacggcca
     3061 ggggatccga tgcaccctcg ctctcgccgg ggagtttgcg aggacccccg gccaggcagg
     3121 gtcggcggcc gatgggaacc ttcggtgtat cgcgggaatc gccgtggcag ggctggatgc
     3181 ctccctgtcg gtgaagccga gtgccctcgg cggactcttc gatccggagg cctgccgcgg
     3241 gcatgcgacg gggctcgccc gggaggccgc ccggtccggg gtccccctcg agctggacat
     3301 ggaaggaagg aactccgtgg attccaccct cgacatcgcg gccgggtgcc ggagggaaaa
     3361 cccccgggtc atggtagctc tccaggccta cctcgaccgg acccccgggg acatccgcaa
     3421 catggtctcc ctggggatcg gggtccggct cgtcaagggt gcgtatctcg gggacgtgtc
     3481 agactacagg caggtccggg ggcggatgaa ggaatgtgcg ggaatacttc aggatctcgg
     3541 cgcatccttc tccctcggga ctcatgaccc ggtcctcatc ggctgggcca tggaggaatt
     3601 cgcggacggg aaggatctcg tggagttcgg gttcctgaag gggctctccg atgaaacgaa
     3661 gatccgcctc gcgtccgggg gatggagggt ctcggagtac gtcccctacg gcaggggcga
     3721 ggaggggtac gtcacgagga gggaacggta cctcgccaat ctcgcggggg ctggccttgc
     3781 cccggccccg tgagccgccg cgcggcgaat gccggggtat ggtcaccgct tcaggtcacc
     3841 gggccggagg ctccggatga gcgcctcgat cccgacaatc tccctcgtca ggacctcctc
     3901 gtcgaggaac tggcggagct tgaactcgaa tttctccctc acctcccggt tcaggttccc
     3961 ctcttcgtcg aactctttcc tgaggtagtc cttgagttcg ctgaggtcct ttatcgtcaa
     4021 cttgttgaca aggatcaccc ggtatctgtc caggagggat tcccggatcc cggttctccg
     4081 gtgtcttccc tcggaaccgg atccctcacc cttatctgct gccatcggtt cggccatccc
     4141 cttgttcaat ttcctgacac gatgccccga tataagaaca ttgtccaacc tttcaaagaa
     4201 tgcccggaac cgcaatcttc attagtatct ctttcctctc ccttcaccgg tgaaacgtat
     4261 ggccgacacg aagaacttcg gtgaactgat ctacaccttt gacaaggccg cgggagaggc
     4321 gctcgggaag cgcgagagcc acaccccgaa gatcgaggcc cccgccacgg tgaagccgga
     4381 ccaggccttc gacctgaagg tcatggtcgg gccccacccg aataccatcg agcactccat
     4441 ccggtggatc accgtctggg tcaacgagga cgggcgggcc ttcaatccca tcttcatcgg
     4501 gaaggccagc ctcaccccgg tcgtcgccca gcccgaggtg gtcttcaagg tgaagctcca
     4561 gaagggcggc gtgatccacg ccgtggagta ctgcaacctc catggcctgt ggtccgggaa
     4621 gaaggagatc aaggtcggct gatcatcctc ccatcgtttt ttcggtttcc gacgcgggga
     4681 ccggggtccc ggcgtcgcat gcagccctga aggaatccat tcccggatca ggttcaatct
     4741 gtggaacgta cccatgacgg atacgaacgg ggagcaggcc cgcgccatca tgctcgactt
     4801 cgcgcgcctc accggcctgg atccggaggg aagagggccg gggcgctacc tgtggaccga
     4861 cgcctttgcg gtgtgcaact atgtcgggct ctggcaggcg acgggggacg aaggcctccg
     4921 ggatctcgga ctgcgcctgg tggaccaggt gcaccacacc ctcggcaggc accggggtga
     4981 cgacccgagg acgggctggc tgagcggtct cggggaggag gatggagaga gtcatccgac
     5041 catcgggggg ctccggatcg ggaagaagct ccccgagcgg gctcttgggg aaccccccga
     5101 tgagcggctg gaatgggacc aggacgggca gtacttccac tacctgacga agtggatgca
     5161 tgccctcgtc atcgcgggcc gcgccacggg ggacccggtt ttccttcgct gggcggcaga
     5221 gctcgcgagg accgcctatg cagggtttat ccatcgtccc cggtctggag gcccggaccg
     5281 gatgtactgg aagatgagca tcgatctctc ccgtcccctc gttgcttcga tgggacagca
     5341 cgaccccctc gacggccttg tcaccttctg cgagatccag gtcgcccggg gaacctatcc
     5401 cgccccattt cttcccccgg atctcgggga ggagatcgca ggacttgcag ggatctccca
     5461 gggcatggac cttcccaccg acgatcctct cggcaccggc ggcctgatct ccgatgccct
     5521 gcgcatcgcc caggtgatgg tgcagggcgg gggaagattc ccggagcagg ccgtgcctga
     5581 actggatctc ccggccctgc tcggcgaagt cctcaacgcc gcccttctcg gcctggatgc
     5641 cttcgccgac agcgggtgca cggggttacc cgctgatttc cggctcgcct tccgggagct
     5701 cgggctctcc atcgggctct cgggggccgg ggaactgatc aggctgatcg acgggcaccc
     5761 atcgaggttc gggtcaggca gctccctgca ggtg
//
LOCUS       DATGDI010000133         4158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_129176, whole genome shotgun sequence.
ACCESSION   DATGDI010000133 DATGDI010000000
VERSION     DATGDI010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129176"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /gene="thpR"
                     /locus_tag="VKO45_05040"
     CDS             <1..554
                     /gene="thpR"
                     /locus_tag="VKO45_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HMA05274.1"
                     /translation="VFLMVRTFIAIDLTPEVRERMRDPQASLAGCRARLTLVKPPSIH
                     ITLKFLGEIDEKTLEKVKEALSRIRFEPFDISAGGVRGNPPQSPRVVWSEIRDEGGCG
                     RLFDLIEEALSPLHIPRERRGYTPHATLARVKRFDPSLLPVLAGLAAEEFGSCPVRCF
                     RLKKSTLTPGGPVYEDILEVPCA"
     gene            551..1984
                     /gene="cca"
                     /locus_tag="VKO45_05045"
     CDS             551..1984
                     /gene="cca"
                     /locus_tag="VKO45_05045"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HMA05275.1"
                     /translation="MRKRRDEGIGAPGKKGIRAAEAKGIRAGGEKEVLGRIRPGKEEH
                     NHTWAVATEILSAVGEDGRAKGMVVGSVARDTWLSGEKDLDIFLLLPPSLSREELEET
                     GLDLARKIARRFAGSFREKYAEHPYVNATIDGLDVDLVPCYEVASAAKIQSAVDRTPF
                     HTRYIAEKIAPLRDDVLLAKQFAKACGVYGSDQMTEGFSGYLFELLVLHYGGFTPLLE
                     AAAAWKPGTCIDLEGHACKAFEEPLRVVDPVDPARNVAAALSGTRMFEFVEYSRGYLE
                     SPSPDFFFPPGSGGMDREGFARVLARRGTYLYALTCATPPLIPDIVVPQLRRSLASVR
                     GLLVRHGFPVLQDDCAMGEDRSILVLELLVDRLPSVTRHMGPPLWDRANAGKFADKYW
                     GNPFSGPFIRDGRYHVDLDREFTSAGDLLRSPEILSVGLGKHVKQSMERGWEVSEGPD
                     VWRDEFAGFLSRFIEPASPLARLKRGS"
     gene            complement(1989..2222)
                     /locus_tag="VKO45_05050"
     CDS             complement(1989..2222)
                     /locus_tag="VKO45_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HMA05276.1"
                     /translation="MRGPGTDGRLRSSVRVGSTVDVVRKQDQKTGKRTRGIVAEILTS
                     SASHPHGIKVRLRDGTVGRVVGVVGDVEPGKEK"
     gene            complement(2219..2659)
                     /locus_tag="VKO45_05055"
     CDS             complement(2219..2659)
                     /locus_tag="VKO45_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HMA05277.1"
                     /translation="MTAKRSGEAVAAFGRGYNCSMAIFSAFAPDLGLDAEKAARIASP
                     FGAGVARTGEICGAVSGALMVIGLSRRPEEICDPPSREKVYTLAQRFIDEFTARNGSV
                     NCTELVGYDLSDPARYAEAREKKVFATRCSRLVRDAAEILEGIL"
     gene            complement(2714..3151)
                     /locus_tag="VKO45_05060"
     CDS             complement(2714..3151)
                     /locus_tag="VKO45_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HMA05278.1"
                     /translation="MDTATSEPMALKEQIFEVLEGPHPAAVATIDRGMPAVRFMVLNG
                     FWDMTLVGGTMKTSRKVEQLRRNPNAAIAIWSGKEFTDPYVVIQAKGEIHEDVETKKK
                     YWNPMFEQYFKSVENTEFVVLVFTAREIEYHGANMAMPETWKR"
     gene            complement(3281..3745)
                     /gene="msrB"
                     /locus_tag="VKO45_05065"
     CDS             complement(3281..3745)
                     /gene="msrB"
                     /locus_tag="VKO45_05065"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HMA05279.1"
                     /translation="MDAPKKSTGKVPIFSVATGKVELVDRVVKTDEEWRKILDPNTFE
                     IARRAGTEPAFTGKYHAWHEKGLYRCACCGTDLFLSDTKFESGTGWPSFWAPVSEHNV
                     ELRKDITFGMVRTEVLCARCGAHLGHVFDDGPPPTHQRYCMNSASLAFMKRD"
BASE COUNT          742 a         1345 c         1297 g          774 t
ORIGIN      
        1 aagtattctt gatggtcagg acttttattg ctattgatct caccccggag gtgagggagc
       61 ggatgaggga cccgcaggca tcccttgcag gctgccgggc ccggctcacc ctcgtgaaac
      121 ctccctccat ccacatcacc ctcaagttcc tgggggagat cgacgaaaaa accctcgaga
      181 aggtcaagga ggccctctcc cggatacgct tcgagccctt cgatatctcc gccggcgggg
      241 taagggggaa tcccccgcag tccccccggg tcgtctggtc ggagatccgg gacgagggag
      301 gctgcgggag gctctttgac ctcatcgagg aggccctctc ccccctgcac atcccgaggg
      361 agaggagggg gtacaccccc catgccaccc tcgcgcgggt gaagaggttc gatccctccc
      421 tcctccccgt cctcgcgggc cttgccgcag aggagttcgg gtcctgccct gtccgctgct
      481 tccggctgaa gaagtctacc ctcacacccg gaggacccgt ctacgaggac atcctggagg
      541 tgccctgcgc gtgaggaaac ggcgggatga agggatcggg gccccgggga agaaggggat
      601 ccgcgccgcg gaggcgaaag ggatccgggc cgggggcgag aaggaggtcc tcgggaggat
      661 ccggccgggg aaggaggagc acaaccacac ctgggccgtg gcaacagaga tcctctcggc
      721 cgtcggggag gacgggaggg caaaagggat ggtggtcgga tccgtggccc gggacacctg
      781 gctctcgggg gaaaaggacc tggacatctt ccttcttctt cccccctccc tctcccgcga
      841 ggagctggag gagacgggcc tcgacctcgc ccggaagatc gcccgccggt tcgccgggag
      901 cttccgggag aagtacgccg agcatccgta tgtgaacgcg accatcgacg gcctcgacgt
      961 ggacctcgtc ccctgctacg aggtcgcctc cgcggcaaag atccagtccg ctgttgaccg
     1021 cacccccttc cacacccggt acatcgccga aaagatcgca cccctccggg acgacgtcct
     1081 cctcgcgaag cagtttgcca aggcctgcgg ggtctatggc tcggaccaga tgaccgaggg
     1141 tttctcgggc tacctcttcg agctcctcgt gctccattat ggcggcttta ccccactcct
     1201 cgaggcagcc gcggcatgga agcccggcac ctgcatcgac ctcgagggcc atgcatgcaa
     1261 ggccttcgag gagcccctca gggtggtgga cccggtcgac ccggcccgga acgtcgcggc
     1321 cgccctctcc ggcacccgca tgttcgagtt cgtggagtac tcgcggggtt acctggagag
     1381 cccctcgccg gacttcttct tcccgccggg atcgggaggt atggaccggg aaggcttcgc
     1441 ccgcgtcctc gccaggcggg ggacctatct ctatgcactc acctgtgcaa cccctccgct
     1501 cattcctgac atcgtcgtcc cccagctccg gaggagcctt gcctcggtcc ggggtctcct
     1561 cgtcaggcac ggctttccgg tcctccagga tgactgcgcc atgggagagg accggtccat
     1621 ccttgtcctc gagctcctgg tggaccggct cccgtccgtc acccggcaca tgggaccgcc
     1681 gctctgggac cgggcgaacg ccgggaagtt cgcggacaaa tactggggaa atcccttctc
     1741 gggccccttc atcagggatg ggcgctacca cgtggacctg gaccgggagt tcacgagcgc
     1801 cggagacctc ctgcgctccc ccgagatcct ttccgtgggc ctggggaaac acgtgaagca
     1861 gtcgatggag aggggctggg aggtgagcga gggaccggat gtctggaggg atgagttcgc
     1921 ggggttcctg tcccggttca tcgaaccggc gagccccctc gcgaggctga agcggggctc
     1981 gtaaaaattt acttttcttt tccgggttcg acatccccga cgaccccgac caccctcccc
     2041 accgtcccgt ccctgagcct gaccttgatg ccgtgagggt gggaggcgga ggaggtcagg
     2101 atctctgcga cgattccgcg agttctcttt ccagtcttct gatcctgttt ccggaccacg
     2161 tccaccgttg agccgacacg aaccgatgaa cggagccggc cgtcggttcc cggacctctc
     2221 acaggatccc ttccaggatc tccgccgcat ccctcacgag cctcgagcac cgggtggcga
     2281 acaccttctt ctccctcgcc tccgcgtaac gggccgggtc agagaggtcg taccctacga
     2341 gctcggtgca gttcaccgat ccgttccggg ccgtgaactc gtcgatgaac ctctgtgcga
     2401 gggtatagac tttctccctc gatgggggat cgcatatctc ctccggtctc ctggaaaggc
     2461 cgatgaccat gagcgccccc gaaacggcgc cgcaaatctc cccggtccgt gccactcccg
     2521 caccgaaggg ggaggcgatc ctcgccgcct tctccgcatc aagcccgagg tccggggcaa
     2581 atgccgagaa gattgccatg gagcagttgt acccgcgacc gaaggcggcc acggcctcgc
     2641 cggaccgttt ggctgtcacg gatctctatt cgttttccgg ccaaaaaaag gaatggatgg
     2701 tgagggaccg gaatcacctc ttccaggtct ccggcatcgc catgttcgcc ccgtggtact
     2761 cgatctcccg ggcggtgaag acgaggacca cgaactccgt attttcgacg gacttgaagt
     2821 actgttcgaa catcgggttc cagtacttct tcttggtctc cacgtcctcg tggatctctc
     2881 ccttcgcctg aatgaccaca tagggatcgg tgaactcctt ccccgaccag atggcgatgg
     2941 ccgcgttcgg gttccttcgg agctgctcca ccttccggct cgtcttcatg gtcccgccaa
     3001 cgagggtcat gtcccagaat ccgttgagca ccatgaatcg cacggcgggc ataccccggt
     3061 cgatggtggc gacggcggcg ggatgggggc cctccaacac ctcaaaaatc tgctccttaa
     3121 gtgccatggg ttcactcgtg gcggtgtcca tattaaagct gaccccgaaa agcaggggat
     3181 gaaatgatga tgtcccgtcg tgcctgcctg gaagggagga ggccggtatg cgataccgcg
     3241 agacacccga acttcctccc cttcccagag agtggtttct ctagtccctc ttcataaagg
     3301 cgagcgatgc cgagttcatg cagtaccgct ggtgggtcgg cggcgggccg tcatcgaaga
     3361 catgcccgag gtgagccccg caccgggcgc agagcacctc ggtccggacc ataccgaagg
     3421 tgatgtcctt ccggagctcg acgttgtgct ccgagaccgg ggcccagaaa gagggccagc
     3481 cggtgcctga ctcgaacttc gtatccgaga ggaagaggtc ggtcccgcag catgcacacc
     3541 ggtagagtcc cttctcgtgc caggcatggt acttcccggt gaaggcgggc tcggtccctg
     3601 cccttcgcgc gatctcgaac gtgttcgggt cgaggatctt cctccactcc tcgtcggtct
     3661 tcacgacccg gtccacgagc tccaccttcc ccgtcgcgac cgagaagata ggtactttgc
     3721 ctgttgattt cttcggggca tccatggttc tcactccggc tgagagaccg tggggaataa
     3781 gtccctttcg gtgagagagg tttcgaagaa accgacctcg agcgacgtag tcgcgaatag
     3841 ggaggtttca tcagaaaccg acctcgagcg acgtagtcgc gagtgaggga ggtttcgtgg
     3901 gagggagggt tcatcagaac ccgaccttga gcaacgaagg tgcgataagg gagggtccgg
     3961 aggacccgac ctcgagcgac gacgactatt tacgtaggac gatgatttcc gcaggaaatc
     4021 ggagtcgagc gacgtaagtc gcgagcaaat aggagtgccg cgacgggtag tagcggccaa
     4081 gtcgcgagga aaccgacctg gagcaacccg ctatctggcg gggtgcgacc cccggttctt
     4141 ccgcttcgcc ttctccct
//
LOCUS       DATGDI010000134         5616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_187397, whole genome shotgun sequence.
ACCESSION   DATGDI010000134 DATGDI010000000
VERSION     DATGDI010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187397"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VKO45_05070"
     CDS             <1..818
                     /locus_tag="VKO45_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMA05280.1"
                     /translation="VLAQEADPLICLGVGFSKMTNVPLDRPMIQLDIDPQKLGKGPSS
                     VSLWGNCHSVLPKWIEMVAPREDPAAGARIAALKGAIRKRLDAEADAKAIPVRPPFIM
                     KVLSDTIPDDAVISIDIGENGWWFGRNFRMKRQRFVMSGYLASMGFGLPGAIAAKLAR
                     PEKKVFCITGDGGFAMAMAELVTAVKYHLPMVVVVLNNRELGMIRVEQQMEHYPNFGT
                     DLLNPDFAAYGESCGGRGIKVTRPGELEPAIRKAMALDTVVVVDVETDPRRFV"
     gene            988..1707
                     /locus_tag="VKO45_05075"
     CDS             988..1707
                     /locus_tag="VKO45_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HMA05281.1"
                     /translation="MQQRAGTGRSSSEPRGDLLRIDMHVHSSYSQDSIIPPKTIFQSW
                     KRTGIIPLVCDHDSLEGSLRVMRAIHAENTGIPRVLAEEVTTREGEIIGLFLSEEIPP
                     GLSAADTLDRIHGQGGLSLVPHPFCDYRSKVLRAGTRNALVEKIDLVEGHNARTISGE
                     EDRLGIIFASEMGIPFTVGSDAHIPFELGRVWMEVPPFDGPRELLKGLAGAPFSFRRV
                     TRGVHLFTHLVKGVRKLQADR"
     gene            1787..2506
                     /locus_tag="VKO45_05080"
     CDS             1787..2506
                     /locus_tag="VKO45_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HMA05282.1"
                     /translation="MNWQIAGIALTVFLATFLGGLVVFRLKRTLPYLFAFAAGNLIGV
                     SFFDIQPVLFQIGGGSGISMRWFMVIIVASFFFFNLLERYLVLYHTDSEDFHGHIMGP
                     VGAGGLVVNSLLDGVAIGIAFQAGAAIGLIVGLAVIFHDITDGMNTVVIMLKNRQSTR
                     NSMVFLLLDSIAPALGILLTVPLRLPEPFLAILLSIFAGEFLFIGAASLLPETYRYPP
                     MKMVISMGLGILLILGLTLLV"
     gene            complement(2630..3379)
                     /locus_tag="VKO45_05085"
     CDS             complement(2630..3379)
                     /locus_tag="VKO45_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HMA05283.1"
                     /translation="MASLSSRCIAEGVGAALLIYFGAGAAAITLMLAAGTKPATAFNI
                     GIGQLGGLADWLAIGMAFAIVLAVVIYALGRISGAHINPAVTIALWATKRFPALDAVC
                     YIVSQCIGAAIGSLLFFLSAGPAAVTIGGLGATAPFPGIGYGQAILVEAIATFVLMLA
                     IMGVAVDSRAPTGFAGLVIGLTLGAMITATGNLTGGSLNPARTFGPYVMDWLLGGPNL
                     WATFPIYVVGPIIGAVLAAFFYDRITAEGTV"
     gene            complement(3379..3591)
                     /locus_tag="VKO45_05090"
     CDS             complement(3379..3591)
                     /locus_tag="VKO45_05090"
                     /inference="COORDINATES: protein motif:HMM:NF021464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2180 family protein"
                     /protein_id="HMA05284.1"
                     /translation="MKCYVCAKEGKESDAVAVCIACGMATCMKHTIRRDVDVWEGSYP
                     LPSRKLPRKMPRMLCPDCNAAYEGGK"
     gene            complement(3726..4280)
                     /locus_tag="VKO45_05095"
     CDS             complement(3726..4280)
                     /locus_tag="VKO45_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HMA05285.1"
                     /translation="MDLITILLVAVALAMDCFAVALAACIRPEERMRNATRIALAFGL
                     FQGGMPVLGWLAGRTFSDLISGFDHWVAFGLLAIVGLRMIRGGMSADMDQKTPCLDTT
                     HLLFLSVATSIDALAVGVSFAFLDTGILLPCLIIGVSTFTISFLGAVLGGAAAERWGK
                     AMEVLGGLVLLGIGIRILFTHMVH"
     gene            complement(4358..4861)
                     /locus_tag="VKO45_05100"
     CDS             complement(4358..4861)
                     /locus_tag="VKO45_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HMA05286.1"
                     /translation="MIKDNVRDAINTQMNREFYSSFLYLSMAAYFESMNLKGFSRWMQ
                     VQAKEEHAHGMKFFDYLVGQGARITMADIEAPAAEWPTPLAAFEHALGHERKVTGLIH
                     DLVELAMSEMDHATTTMLQWFVTEQVEEEANAGEIVFKMKLASAEKGAVLLYLLDHEL
                     GKRGKTT"
     gene            complement(4947..>5616)
                     /locus_tag="VKO45_05105"
     CDS             complement(4947..>5616)
                     /locus_tag="VKO45_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMA05287.1"
                     /translation="VNTFYSIHGRVLPVATGIKLANPDLTVIGCAGDGDAYAEGLDHL
                     IFAAKRNLDISLFIHNNRVYGLTTGQYTPTSPLGFKGRSTPGGTPEYPLNPLELMFAS
                     GATYIARGYTRNQHLLKKTMAEAVRHKGFSFVDILQICATYFDVHDVYDKYVYELAGH
                     DTKNPQQALQKILEWDYNIPAPIALGTLYEKDLPSFESRFARQPASPADRRGKIRALL
                     DSFR"
BASE COUNT         1049 a         1750 c         1755 g         1062 t
ORIGIN      
        1 gggtcctcgc ccaggaggcc gaccccctca tctgcctggg ggtcgggttc tcgaagatga
       61 ccaacgtccc cctggaccgg cccatgatcc agctggacat cgacccccag aagcttggca
      121 agggcccctc gtcggtaagc ctctggggca actgccactc cgtcctcccg aaatggatcg
      181 agatggtcgc gccgagggag gacccggcgg cgggggcaag gatcgcggcc ctgaaggggg
      241 cgatccggaa acggctcgat gccgaggccg acgcgaaggc catcccggtc cgccccccct
      301 tcatcatgaa ggtgctctcg gacaccatcc ccgatgatgc cgtcatctcc atcgacatcg
      361 gggagaacgg gtggtggttc ggccggaact tccggatgaa gcggcagcgc ttcgtgatgt
      421 ccggctacct cgcctccatg ggcttcggcc tccccggtgc catcgccgca aagctcgccc
      481 ggccggagaa gaaggtcttc tgtatcaccg gcgacggcgg cttcgccatg gccatggccg
      541 agctcgtgac cgccgtgaag taccacctcc ccatggtggt cgtcgtcctg aacaaccggg
      601 agctcgggat gatccgggtg gagcagcaga tggagcacta cccgaacttc gggaccgacc
      661 tcctcaaccc ggacttcgcg gcctacgggg agtcctgcgg ggggagggga atcaaggtca
      721 cccggcccgg cgaactcgag cccgccatcc ggaaggcgat ggccctcgac acggtggtgg
      781 tggtggacgt ggagaccgac ccgaggcggt ttgtataacc cgtccaggtc cgaatcttcc
      841 cgggacctaa ccccgccacc cgcccctccg ccctgccatc cctcttaacg atagcttccc
      901 ctctctcagg cgtccctcgc cgaaacaggt ataacccccc gggataacgg tatcctataa
      961 gcaattcccc cggggtcgtt tcacctgatg cagcagaggg cgggaacggg aaggagctct
     1021 tctgaacccc gcggcgacct cctgcgtatc gacatgcacg tgcactcctc ctattcccag
     1081 gactccatca tcccgccaaa gaccatattc cagtcctgga agaggaccgg gatcatcccc
     1141 ctcgtctgcg accacgactc cctggagggc tccctccggg tgatgcgggc catccatgcc
     1201 gaaaatacgg gcatcccccg ggtcctcgcc gaggaggtca ccacccggga gggggagatc
     1261 atcgggctct tcctctccga ggagatccct ccggggctgt ccgccgccga taccctggac
     1321 cgcatccacg ggcagggcgg gctctcgctg gtccctcacc ccttctgcga ttaccggtcg
     1381 aaggtgctcc gggcggggac gaggaacgcc ctcgtggaga agatcgacct cgtggagggg
     1441 cacaacgccc gcaccatctc cggggaagag gaccggctgg ggatcatctt cgcctcggag
     1501 atggggatcc ccttcacggt ggggtcggat gcccacattc ccttcgagct cgggagagtc
     1561 tggatggagg tccctccctt cgacggcccc cgtgagctcc tgaaggggct tgccggggcg
     1621 cccttcagct tccggagggt aacccgcggg gttcacctgt tcacccatct ggtgaaaggg
     1681 gtccggaagc tccaggccga caggtaggat ctcagctgac gggcgatctt ttcattccgg
     1741 aggaaagagg cagggttttt taccggcccg tccacagtat ggatggatga actggcagat
     1801 cgccggtatc gcgctcacgg tgttcctcgc gaccttcctg ggcgggctcg tcgtgttccg
     1861 gctgaagagg accctcccct acctcttcgc ctttgccgcc ggaaacctca tcggggtgtc
     1921 ctttttcgat atccagccgg tgctcttcca gatcggggga ggctcgggga tctccatgag
     1981 atggttcatg gtcatcatcg tcgcctcctt cttcttcttc aacctgctgg agaggtacct
     2041 cgtcctctac cacaccgaca gcgaggactt ccacggccac atcatggggc ccgtgggtgc
     2101 cgggggcctc gtggtgaaca gcctcctcga cggggtggcc atcggcatcg ccttccaggc
     2161 gggggcagcc atcggcctca tcgtcggcct tgccgtgatc tttcatgaca tcaccgacgg
     2221 gatgaacacg gtggtgatca tgctgaagaa ccggcagtcg acaaggaatt ccatggtgtt
     2281 cctcctcctg gattccatcg cccccgccct cgggatcctc ctcaccgttc ccctccggct
     2341 cccggagccc ttcctcgcca tcctcctttc catcttcgcg ggcgagttcc tcttcatcgg
     2401 ggcggcgagc ctcctgcccg agacctaccg gtacccgccc atgaagatgg tgatctccat
     2461 ggggctcggg atcctcctga ttctcggcct cacgctcctg gtttagggag atccggggaa
     2521 aaggtgatgg catcctcgtt atctcaaaat aaataactgt gctaccaccg tttcaattga
     2581 aaccgttgaa cctgctcacg atggaaatgg aaagaaatgg gggagaggat tatacggttc
     2641 cctcggcggt gatccggtca tagaagaacg cggcgagaac cgcgccgatg atgggcccca
     2701 cgacataaat ggggaaggta gcccagaggt tcgggccgcc cagcaaccag tccatcacgt
     2761 agggcccgaa ggtcctcgcg gggttgaggg accccccggt cagattccca gttgccgtga
     2821 tcatggctcc cagcgtgaga ccgatgacaa gcccggcaaa cccggtgggg gcacggctgt
     2881 caacggcaac gcccatgatg gcgagcatca ggacgaaggt tgcgatggcc tcgacgagga
     2941 ttgcctgccc gtacccaatc ccggggaagg gtgccgttgc tccgagccct ccgatcgtga
     3001 cggcggccgg ccccgcggag aggaagaaga gaaggctccc gatggccgca ccgatgcact
     3061 gggacacgat gtagcagact gcatcgaggg caggaaaccg ctttgtcgcc cagagggcaa
     3121 tggtcaccgc agggttgatg tgggcgccgg atatccggcc gagagcatag atgaccaccg
     3181 cgaggacgat ggcaaaggcc atcccgatgg ccagccagtc tgcgagcccg ccgagctgtc
     3241 cgattccgat attgaaggcc gtcgccggtt tcgtgcccgc ggcgagcatg agggtgatgg
     3301 ccgcggcccc cgccccgaag tagatcagga gtgccgcgcc cactccctcc gcgatgcagc
     3361 gggaagaaag cgatgccatc acttccctcc ttcataagcg gcgttgcaat cagggcagag
     3421 catccgtggc atcttcctgg ggagcttacg ggagggaaga ggataacttc cctcccagac
     3481 atccacatct ctccggatcg tatgtttcat gcacgtcgcc ataccgcagg caatacatac
     3541 ggccacagca tcactttctt ttccctcttt tgcacaaaca tagcacttca ttctgttcac
     3601 ctcgcatctg ctgttttaaa aacacaaatg aatacactat cgggttcatg ttataatctt
     3661 gcttattttt cccccttcag gatactattt gaccttaaac gaagcagagc cgtcctgtta
     3721 tgcgcttaat gcaccatgtg tgtgaagagg atccggatcc cgatcccgag gagaaccagg
     3781 cccccgagca cctccatcgc ttttccccat ctctctgcag ccgctccccc taggacggct
     3841 ccgaggaaag agatagtgaa ggtggaaacc ccgatgataa ggcacgggag gagaattccg
     3901 gtgtcgagga aggcaaagga gactcccacg gccagggcat cgatggaggt cgccaccgag
     3961 aggaagagga ggtgggtggt atccaggcag ggggttttct ggtccatgtc agcggacatc
     4021 ccgccccgga tcatccggag ccccacgatg gcgaggaggc cgaaggcgac ccagtgatca
     4081 aagccggaga tgagatccga gaacgttcgc ccggccagcc atcccaggac cggcattcct
     4141 ccctggaaca ggccaaacgc aagggcgatt ctcgtggcgt tcctcatccg ctcctcgggc
     4201 ctgatgcatg cggcgagggc cacggcaaag cagtccatcg caagggcgac agcgacgagg
     4261 aggatggtga tgagatccat ggggggaact gattcccttg tgagccctcg gaatcgaccg
     4321 gcgaaaagga aatgaggtcc ggggaggggg acccggttca ggtggtcttc ccgcgtttgc
     4381 cgagctcgtg gtccaggagg tagaggagca ccgcaccctt ctctgccgag gcgagcttca
     4441 tcttgaagac gatctcaccg gcgtttgcct cctcttccac ctgctccgtg acaaaccact
     4501 ggagcatcgt ggtggtggca tggtccatct cggacatggc gagctccacg aggtcatgga
     4561 tcaggccggt gaccttccgc tcgtgtccga gggcatgctc gaaggccgcg aggggggtcg
     4621 gccactcggc ggcgggggcc tcgatatcgg ccatggtgat cctcgccccc tgcccgacca
     4681 ggtagtcgaa gaacttcatc ccgtgggcat gctcctcttt tgcctggacc tgcatccagc
     4741 gggagaatcc cttcaggttc atggactcga agtaggccgc catggagagg tacaggaagg
     4801 aggaatagaa ttccctgttc atctgggtgt tgatggcatc ccggacattg tccttgatca
     4861 tggtactagc ctcccatccc tgtgggggat ggatctatat aagcctggac gtgggggacg
     4921 ggatcccgga cggggcggga tcccggtcac cggaaggagt cgaggagtgc ccggatcttc
     4981 cctctccggt ccgctggcga ggcaggctgc cgggcgaacc ggctctcgaa gctcgggagg
     5041 tccttttcgt acaatgtccc gagggcgatg ggtgcaggga tgttgtaatc ccattcgagg
     5101 atcttctgga gggcctgctg gggatttttc gtatcatggc ccgcgagctc gtacacgtac
     5161 ttgtcgtaga cgtcgtggac atcgaagtag gtggcgcaga tctggaggat gtccacgaag
     5221 gagaacccct tgtgcctgac cgcctcggcc atggtctttt tcaggaggtg ctggttacgg
     5281 gtatagcccc tcgcgatgta ggtggcaccg ctcgcgaaca tcagttcgag ggggttgagg
     5341 ggatactccg gggtcccccc gggggtcgac ctccccttga acccgagggg ggaggtgggg
     5401 gtgtactgcc ccgtggtgag cccgtacacc cggttgttgt ggatgaagag gctgatgtcc
     5461 aggttcctct tcgcagcgaa gatgaggtgg tcgagcccct cggcatacgc atccccgtcc
     5521 ccggcgcacc cgatgacagt gaggtccggg ttcgcgagct tgatccccgt ggccacgggg
     5581 aggacccggc catggatgga gtagaaggtg ttcacg
//
LOCUS       DATGDI010000135         1899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_303954, whole genome shotgun sequence.
ACCESSION   DATGDI010000135 DATGDI010000000
VERSION     DATGDI010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303954"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VKO45_05110"
     CDS             complement(<1..858)
                     /locus_tag="VKO45_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HMA05288.1"
                     /translation="MKKEKKSEKTVMQETKPVERKTGEVKSGETKAGERKSPRPIWSG
                     AVSIGLVNVPVKVFPMTRDLSVHFHLLHRKDGQPLKYERVCTRDGVVVPWEETVRGYE
                     VRKNEFIVLEKEELRAALPESDSRIRIDKFVHYLSLDPVYFENSFVLVPDGSADAYAL
                     LYTVCQELGEAGVGHFTMRTKEYPAVVHAYHGGLVLTTLRYANEVMDPSGFEELKALS
                     APAGKQLDLAKRIITDLTGDLDITEYHDAYRERVLAVIDKKRTGETIRVEKPKAAEVK
                     ELMVALEETV"
     gene            complement(855..1730)
                     /gene="ligD"
                     /locus_tag="VKO45_05115"
     CDS             complement(855..1730)
                     /gene="ligD"
                     /locus_tag="VKO45_05115"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HMA05289.1"
                     /translation="MAEPLTRVEFTHLPKIIYPEAGITKLQVIKYFIRMAPRILPFLD
                     GRPVVLTRYPDGVGGKGFYEKDAPMGTPEWVETWPHYSETADRVVHFIVCNSLDTLLW
                     IANLDALEIHMILAPTRSPENPDILFIDLDPEAPATTAQAAGIALLVRDRLASQGIRI
                     SVKTSGKRGFHLAVPLVAGLTYREVRDFVHGMGRELARENALVASEAPGPSNPGRVLV
                     DYGQNSPGRTMVAPYSLRATPRATVSAPVTWREMEKGINPGDLHIQSVPERDEDPWEG
                     ILEHPQRLPGGGRTG"
     gene            1799..>1899
                     /locus_tag="VKO45_05120"
     CDS             1799..>1899
                     /locus_tag="VKO45_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMA05290.1"
                     /translation="MEAVVTGGCGFIGSHLVNALVGRGEKVTVIDDL"
BASE COUNT          292 a          609 c          608 g          390 t
ORIGIN      
        1 gacggtctcc tcgagagcga ccatgagttc cttcacctcg gctgccttgg gcttctccac
       61 cctgatggtc tccccggtcc gtttcttgtc gatgacggcg aggacccgct cccggtaggc
      121 gtcatggtat tcggtgatgt cgagatcgcc cgtgaggtcc gtgatgatcc gcttcgcgag
      181 atcgagctgc tttcctgccg gggcggacag ggccttcaat tcctcgaagc cggagggatc
      241 catgacctcg tttgcgtacc ggagggtggt gaggacgagt ccgccgtggt acgcgtggac
      301 cacggcggga tactccttgg tcctcatggt gaagtgcccg acccccgcct ctccaagctc
      361 ctggcagacg gtgtagagaa gggcgtaggc gtcggccgac ccgtcgggga cgaggacgaa
      421 ggagttctcg aagtacaccg ggtcgaggga gaggtagtgg acgaacttgt cgatccggat
      481 ccggctgtcg gactcgggga gggctgcgcg gagctcctcc ttctcgagca cgatgaactc
      541 gttcttcctt acctcgtagc ccctcaccgt ctcctcccag ggtaccacca ccccgtcgcg
      601 ggtacagacc cgctcgtact tcaggggctg cccgtccttc cggtggagaa ggtggaaatg
      661 gaccgagaga tcccgggtca tggggaagac cttgaccgga acgttcacga gcccgatgct
      721 cacggccccg ctccagatgg gtctcgggct cttcctctcc ccggccttcg tctctccgga
      781 cttcacctct cccgtcttcc tctcgacggg cttcgtctcc tgcatcaccg tcttttcgct
      841 cttcttttcc ttcttcatcc tgttctccct cctccgggaa gcctctgggg atgttcgagg
      901 attccctccc atgggtcctc atcccgttcg gggacactct ggatatggag gtccccgggg
      961 tttatccctt tttccatctc cctccaggtg accggtgccg agaccgttgc ccgcggggtc
     1021 gccctcagac tgtagggtgc caccatggtc cttccggggc tattctgtcc gtagtccacg
     1081 aggaccctgc ccgggttcga gggcccgggg gcctcggagg ccacgagggc attctcccgg
     1141 gccagctccc ggcccatacc gtgcacgaaa tcccgcacct cccggtaggt gagccccgcg
     1201 acgagcggaa ccgccaggtg gaaccccctt ttccccgatg ttttcaccga gatccggatc
     1261 ccctgcgagg cgagccggtc ccggacgagg agggcgatcc cggcggcctg tgcggtggtc
     1321 gccggggcct ccgggtccag gtcgatgaag aggatatccg ggttctcggg cgaccgggtg
     1381 ggggcgagga tcatgtgaat ctcaagggca tcgaggttcg cgatccagag gagggtgtcc
     1441 aggctgttgc acacgataaa atggaccacc cggtccgccg tttcggagta gtgcggccag
     1501 gtctcgaccc attccggggt ccccatcggg gcgtccttct cgtagaagcc cttccccccc
     1561 accccgtcag ggtaccgggt gaggacgaca ggccttccgt cgagaaaagg gaggatgcgg
     1621 ggcgccatcc ggatgaagta cttgatcacc tggagcttcg tgatccctgc ctccgggtag
     1681 atgatcttcg ggaggtgcgt gaactccacc cgggtgaggg gctctgccat cagggacacc
     1741 gccgcccgcc tttcatgtga acaccggtat tttacatggg gagggcaaat aagctctcat
     1801 ggaagcggtg gtcaccgggg gctgcgggtt catcggctcg catctggtga acgccctcgt
     1861 cggccggggg gagaaggtca ccgtgatcga tgacctgag
//
LOCUS       DATGDI010000136         7329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_129369, whole genome shotgun sequence.
ACCESSION   DATGDI010000136 DATGDI010000000
VERSION     DATGDI010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129369"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1670)
                     /locus_tag="VKO45_05125"
     CDS             complement(165..1670)
                     /locus_tag="VKO45_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA05291.1"
                     /translation="MEQRFFFITRVPDEPGSLEKAAAIITEGNGNINRIHYDRRIDPC
                     TVFFEVTADEGMEERIREGLREKGFLQATLPSPGLLRVAVHLPHRPGALHEFLGHTTA
                     ARANIAFVDFDDSGGGPKRLVMAMTLEDRSVAGGLLDRLKSRYRIEVLESVEGGDPLD
                     DTVFYLRFAEDLRSLLGGGEEAFLLRLLHDVNHIVQELNRRGEDPKTVFQSILATGKT
                     LRETSGKGFYVDVQRFPLAGDLTLTCLQPPCGGNIYLLSSPGHLLMVDTGFGIYHDDL
                     LRTFTRLGLGDPRNLSAILVTHADADHCGGAGLFPAVARLTRTSLECIRARDRAFGSR
                     SEGLILETVYTTLINLFSGFRPPRELEILPEPGPAVTGFFPVISRFTLAGHAFEILQG
                     LDGHMAGQVYLASRELGVLFTGDSLIHLEGLTPERRSFNSLAVNLITSVNVDRELARR
                     EREGLLALAASWDRDVPAGGRRCLICGGHGPVSVLEGRSLVTRGEVERCRP"
     gene            1895..4174
                     /locus_tag="VKO45_05130"
     CDS             1895..4174
                     /locus_tag="VKO45_05130"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMA05292.1"
                     /translation="MVNGKRFAKILALLVLIGLIGFVHAQTTATQNGYIEVGLYPYAD
                     FSGTPLSGAPPLAVAFTDLSSGSTPRTYVWEFGDGGTSTDANPTHTYTASGVYTVTLT
                     ITNGYGTNTATKQEYVRVGVGPLADFMGTPQTGNLPLAVTFTDLSTGNPATWSWTFGD
                     GGTSTLQNPSHTYSKAGTYTVRLTVTNSFGTSTKTRSGYITTGMPPHADFTSGARVGA
                     TPLTVAFTDASTGAPTSWSWNFGDGTSSTTKNPAHTYTVAGTYTVTLTVKNAYGTDTA
                     TKAGYVTAGGMPAADFTANPRVGTAPLTVTFTDTSTGNPVSWAWKFGDGSTSSEENPV
                     HVYQNEGTYDVTLTVTNAYGTDTETKTGAGENCIAGVTGYIVVGRVPVADFSATPTSG
                     SAPLAVAFTDKSAGASPRTYSWSFGDGGTSTDTNPTHTYAANGVYSVTLTVTNPFGTD
                     TATKSEYISVGAGPVADFVGTPQSGLLPVAVTFTDQSTGNPASWRWDFGDGAVSNEKN
                     PSHTYTKAGTYAVRLTVANPFGTSTKIKAGYITTGMPPTADFTSGARSGGVPLSVAFV
                     DASKGGPTSWSWSFGDGSTSTTRNPAHTYTVAGLYTVSLTVKNAYGTDTATKAGYVNA
                     AGRPVADFTADERRGVKPFTVRFTDLSSGNPTKWLWNFGDANTSTEQNPVHVYTHEGS
                     YDVTLTVSNQYGTDTVKKTGATPLQTTPAATAAQTTAPSGGSTTSSTTGPQATSTPGF
                     EGILAITGLCALVYLAKRH"
     gene            complement(4276..5037)
                     /locus_tag="VKO45_05135"
     CDS             complement(4276..5037)
                     /locus_tag="VKO45_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA05293.1"
                     /translation="MTPGIAIYVLWLREMTRFVRAKSRVIGTLGMPLFFMAFLGFGFS
                     RAQLPNLPPGVNYIQFLVPGIVGMTLLFSSTFAGISVLWDREFGFLKEIMVAPVSRVS
                     IVLGRIAGGATTSLIQGAAILLLSVPMGFSPAGAAGILLSVLVMLLISVIFIGLGLVF
                     ASNMKDIHGFNMITNFIVFPLFLLSGALFPVGNLPSQVQTFAYLDPLTYGVDALRGAL
                     LGTSTFPLLLDLGVLVLASLVMVLLAAFFFERCRC"
     gene            complement(5034..6029)
                     /locus_tag="VKO45_05140"
     CDS             complement(5034..6029)
                     /locus_tag="VKO45_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA05294.1"
                     /translation="MNPAIAVENLTRRFEDLTAVDNVTFSVEAGELFALLGPNGAGKT
                     TIINMLITLLPPSSGTASVAGFDVLREKDAVRRHIGIVFQEPALDKDLTGRENLDFHG
                     MMYGLSRAAREQRIAEVLELVELGERADVLVQNYSGGLKRRLEIARGLIHHPSVLFLD
                     EPTLGLDTQTRRRIWEYIRRMNREEGVTIILTTHYMEEADYLSGRVAIIDQGRVVAMG
                     TPSALKDILGGDVITLEVGGNGEGLLPLLKSIPWVKGAKLHNGEISVTVDHGERKVPE
                     LVSLARDHGITISSVLLRKPSLEDVFIHFTGRSIREREVSAIESRKELITRRMLR"
     gene            complement(6086..6520)
                     /locus_tag="VKO45_05145"
     CDS             complement(6086..6520)
                     /locus_tag="VKO45_05145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMA05295.1"
                     /translation="MDLYAPAGSRVVAIEEGTVISAGIFTGPDLVPYWNRTYQVTVAH
                     VSGIFCRYAELGDVAVRKGGRVEGGTLIGHVGEVLNPAMIGADSPPYIRALRDQGRTS
                     MLHLEVFDSVPAKSLEYRGGNWFSRARPPGLVDPIRILRDIP"
     gene            complement(6765..7184)
                     /locus_tag="VKO45_05150"
     CDS             complement(6765..7184)
                     /locus_tag="VKO45_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05296.1"
                     /translation="MDNVGYILMDRSRVLDGEGNEEEVLTLHIRGMRFGVRNSDMQDL
                     LATGAQARVERLRKNWRFYLDGVEGLCGLSRSGKAVNVELFGAERYTISLRALRSVLS
                     GGSRFAMVAAVPGEKHQETSRRSPATYQQRIPVTVAS"
BASE COUNT         1380 a         2398 c         2361 g         1190 t
ORIGIN      
        1 agctccccgg cgcaggagcc ctctaccccg aagccttccc cgacgacaga atccccgtct
       61 tcgagtccca ggaccgcctt catgaagtta ccatacagag tggtggaccg gatgtattaa
      121 cgatgcggat gccatgcaaa aagagccccc ttgagacggg ttacctacgg gcggcagcgc
      181 tccacctcac cacgggtgac gagggatctc ccctcgagga ccgacaccgg cccgtgaccg
      241 ccgcagatga ggcagcgcct ccctccggcg ggtacatccc ggtcccagga ggccgcgagg
      301 gcgaggagcc cctcccgctc ccgccgggca agctcccggt ccacgttcac cgaggtgatg
      361 aggttcacgg cgagggagtt gaaggagcgg cgctccgggg tgaggccctc caggtggata
      421 aggctgtcgc cggtgaagag gactccgagc tcccgggagg cgaggtacac ctgcccggcc
      481 atgtgcccgt cgaggccctg caggatctcg aaggcatgcc ccgcgagggt gaaccgggag
      541 atgacgggaa agaatcccgt gacggccggg cccggctcag ggaggatctc cagctcccgc
      601 gggggcctga accccgagaa gaggttgatg agggtggtgt acacggtctc gaggatgagt
      661 ccctcgctcc gggacccgaa ggcccggtcc ctcgcccgga tgcactcgag ggaggtccgg
      721 gtgagccttg cgaccgccgg gaaaagcccg gccccgccgc agtggtcggc atcggcgtgg
      781 gtgacgagga tggccgaaag gttccgggga tccccgaggc cgagccgggt gaaggtccgg
      841 aggaggtcgt cgtggtagat cccgaagccg gtatccacca tgagaaggtg gcccggagag
      901 gagaggaggt agatgttccc gccgcacggg ggctggaggc aggtgagggt gaggtccccg
      961 gcgaggggaa acctctgcac gtccacgtaa aagccctttc ccgaggtctc ccggagggtt
     1021 ttcccggtgg caaggatgct ctgaaacacg gtcttcgggt cctcgccccg ccggttgagc
     1081 tcctggacga tgtggttcac gtcgtggagg agccggagga ggaaggcctc ctccccgccg
     1141 ccaaggaggg acctgagatc ctcggcgaac ctgaggtaga agacggtgtc gtccaggggg
     1201 tcccctccct ccacggactc gagaacctcg atccggtacc gggatttcag ccggtcgagg
     1261 aggcccccgg ccaccgaccg gtcctcgagg gtcatggcca tcacgaggcg tttcgggccc
     1321 ccgcccgagt catcgaagtc gacaaaggcg atgttcgccc ttgcggccgt ggtatggccg
     1381 aggaactcgt ggagggcccc gggccggtgc gggaggtgga cggcgaccct gagcaggccg
     1441 ggggagggca gggttgcctg gaggaatccc ttctcccgga ggccctcgcg gatccgctcc
     1501 tccatcccct catcggccgt tacctcaaag aagaccgtgc agggatcgat cctccggtcg
     1561 taatggatcc ggttgatgtt cccgttgccc tccgtgatga tggccgccgc tttctcgaga
     1621 gacccgggct cgtccggcac ccgggtaata aagaagaacc tctgctccat cccccacctg
     1681 gccctgtagc cagttacgcg gggaatatga aaattcttcc ctcccggtgg gcccggaacg
     1741 ggatctctcc gacgaacaga gataaataca tatccataat tttatgagat aggagattga
     1801 tagcagcatg gtatttctca ctccggatcc tgtgactcgg gatgcacctg atggtccggg
     1861 agggtgaaat cgcatctgat cggaggattg caccatggtg aatggaaaaa gattcgcgaa
     1921 gatccttgct cttctcgtcc tcatcggcct catagggttt gtccatgccc agaccacggc
     1981 gacccagaac ggttacatcg aggtcgggct ctatccgtac gcagacttct cgggaacccc
     2041 gctctccgga gccccgcccc ttgccgtggc cttcacggac ctgtcgagcg ggtcgactcc
     2101 ccggacctat gtgtgggagt tcggtgacgg gggcacctcg accgatgcca acccgaccca
     2161 cacgtacacc gcgtccggtg tctacacggt taccctgacc atcacgaacg ggtacggtac
     2221 caacaccgcg accaagcagg agtatgtcag ggtcggggtg ggccccctcg cagactttat
     2281 gggaactccc cagaccggaa acctccccct cgccgtgacc ttcacggatc tctccaccgg
     2341 gaacccagcc acctggtcct ggaccttcgg tgacgggggg acatcgaccc tccagaaccc
     2401 gtcccacact tactcgaagg cagggaccta cacggtacgg ctcacggtga ccaacagttt
     2461 cggcacctcg acgaagaccc ggtcgggcta catcaccacc ggcatgccgc cccacgccga
     2521 tttcacctcg ggggcacggg tcggggccac tcccctcacc gttgccttca cggatgcatc
     2581 gacaggggcc cccacctcct ggtcctggaa cttcggtgac gggaccagct ccaccaccaa
     2641 gaacccggcc cacacctaca cggtcgccgg cacgtacacc gtgaccctca cggtgaagaa
     2701 cgcgtacggg accgacacgg cgaccaaagc gggatatgtc accgccggcg gtatgcccgc
     2761 ggctgacttc accgcaaacc cgagggtcgg caccgccccc ctcaccgtga ccttcaccga
     2821 tacctcgacc gggaacccgg tatcatgggc ctggaaattt ggcgacggga gcacatcctc
     2881 cgaagagaac cctgttcacg tgtaccagaa cgagggcacc tatgacgtga ccctcacggt
     2941 cacgaatgcc tacggcaccg acaccgagac gaagaccggc gcgggcgaaa actgcatcgc
     3001 gggcgtgacc ggttatatcg tcgtgggacg ggtgccggtg gcggacttct cggcgacccc
     3061 gacctcgggt tccgcgccgc tcgccgtggc cttcacggac aagtctgccg gcgcctcgcc
     3121 gaggacctac tcctggagct tcggtgacgg cggcacctcc accgacacga atccgaccca
     3181 cacctatgcg gccaacgggg tctactccgt caccctgacc gtgacgaacc ccttcgggac
     3241 ggacacggca accaagtcgg agtacatcag cgtgggagcc gggccggtcg ccgacttcgt
     3301 ggggactccc cagagcggcc tcctccccgt cgccgtgacc tttaccgacc agtcgaccgg
     3361 gaacccggcc agctggcggt gggacttcgg tgacggggcc gtctcgaacg agaagaaccc
     3421 gtcccacacc tatacgaagg cagggaccta cgcggtccgg ctcacggtag cgaatccctt
     3481 tggcacatcg acgaagatca aggccgggta catcacgacc ggcatgccgc cgaccgccga
     3541 cttcacctcc ggggcgaggt cagggggcgt cccgctgtcc gtcgccttcg tggatgcctc
     3601 gaagggagga cccacctcct ggtcctggag cttcggtgat ggaagcacct ccaccacccg
     3661 gaacccggcc cacacctaca cggtcgccgg gctctacacc gtgagtctca ccgtgaagaa
     3721 cgcgtacggg accgacacgg cgaccaaggc gggatatgtc aacgcggccg ggagacccgt
     3781 ggccgacttc accgccgatg agcggcgggg cgtgaagccc ttcaccgtgc ggttcaccga
     3841 cctctcgtcc gggaacccca ccaaatggct gtggaacttc ggtgacgcga acacctccac
     3901 cgagcagaac ccggtgcatg tctacaccca cgaggggtcc tatgacgtga ccctcacggt
     3961 ttcgaaccag tacggcaccg acaccgtgaa gaagacggga gcgacccctc tccagaccac
     4021 gccggccgcc acggccgccc agaccaccgc tccctcggga gggagcacga cctcctccac
     4081 caccggcccc caggcgacct cgacccccgg gtttgagggg atcctcgcca tcaccggtct
     4141 ttgcgccctc gtgtacctcg caaagaggca ctagaatatt tttctttttt tcccggattg
     4201 tttacatcac cgggccgttt ttctgttact ccgctccggt gaccaggcca ttgggaacgg
     4261 tcgcatccca ctcttttagc accggcaccg ctcgaagaag aaggccgcga ggaggaccat
     4321 gacgagggac gcgaggacga gcacccccag gtcgaggagg aggggaaagg tggaggtccc
     4381 gaggagggcg ccccggagag cgtccacgcc ataggtgagg ggatccaggt acgcgaaggt
     4441 ctggacctgg gagggcaggt tcccgaccgg gaagagggcc cccgaaagga ggaagagggg
     4501 aaagacgatg aagttcgtga tcatgttgaa gccgtggatg tccttcatgt tcgaggcgaa
     4561 cacgagcccg aggccgatga agatgaccga gatgaggagc atgacgagga ccgagaggag
     4621 gattccggcc gctcctgcag gggagaaccc cataggaacg gagaggagga ggatggccgc
     4681 cccctggatg agggatgtgg ttgcccctcc ggcgatccgt ccgaggacga tggagacccg
     4741 gctcaccggc gcgaccatga tctcctttaa aaagccgaac tcccggtccc agaggaccga
     4801 gatccccgcg aaggtggagg agaagaggag ggtcatcccg acgatcccgg gcacgagaaa
     4861 ctggatgtag ttcacgccgg gagggaggtt ggggagctgg gcccgggaga agccgaagcc
     4921 aaggaaggcc atgaagaaga ggggcatccc cagggtcccg atgacccggg actttgccct
     4981 gacgaagcgg gtcatctccc ggagccagag aacgtagatg gcgatcccgg gggtcaccgg
     5041 agcatcctcc tcgtgatgag ttccttcctc gactcgatgg ccgagacctc ccgctcgcgg
     5101 atggaccggc cggtgaagtg gatgaagacg tcctcgaggc tcggtttccg gaggaggacc
     5161 gaggatatcg tgattccatg gtcccgggcg agggagacga gctcggggac cttccgttcc
     5221 ccgtggtcga cggtcacgct gatctccccg ttgtgcagct tcgccccctt tacccagggg
     5281 atcgacttga ggagggggag gagcccttcc ccattgccac ccacctccag ggtaatgaca
     5341 tcgcccccca ggatatcctt cagggctgac ggggtaccca tggcaacgac cctcccctgg
     5401 tcgatgatgg cgaccctccc ggagaggtag tccgcctcct ccatgtagtg ggtggtgagg
     5461 atgatggtga ccccctcctc ccggttcatg cgccggatgt actcccagat cctccggcgg
     5521 gtctgggtat cgaggcccag ggtcggctcg tcgaggaaaa gcaccgacgg gtggtggatg
     5581 aggccccggg cgatctcgag cctccgcttc agcccccccg agtagttctg gacgaggaca
     5641 tcggcccgct cgccgagctc cacgagctcc aggacctcgg cgatcctctg ctcccgggcc
     5701 gcccgggaga gtccgtacat catcccatgg aagtcaaggt tctcccggcc ggtcaggtcc
     5761 ttgtcgaggg ccggctcctg gaagacgatc ccgatgtggc gcctgaccgc gtctttctcc
     5821 cggaggacgt cgaagcccgc caccgaggcg gtacccgagc tgggagggag gagggtgatg
     5881 agcatgttga tgatggtggt cttcccggcg ccgttcgggc cgaggagggc gaagagctcc
     5941 cccgcctcca cggagaaggt gacgttgtcc accgcggtca gatcctcgaa ccgtctcgtc
     6001 aggttctcaa cggcgatggc cggattcata ctccctcccg ggatcttcgc cccggggggc
     6061 cataaacgtg ccccggggcc gggtatcagg gaatgtcccg gaggatccgg atgggatcga
     6121 cgagacccgg gggccttgcc cgggaaaacc agttcccgcc cctgtattcg agagatttgg
     6181 caggcaccga gtcgaacacc tcgaggtgaa gcatgctcgt ccgcccctga tcccggaggg
     6241 cacggatgta tgggggtgaa tcggccccga tcatggcggg gttgagcacc tcaccgacgt
     6301 gcccgataag ggtgccccct tcaacccggc ctcctttcct gaccgcaaca tccccgagct
     6361 cggcatacct gcagaagatt cccgagacgt gggcgacagt gacctggtac gtcctgttcc
     6421 agtaagggac gaggtccggg ccggtgaata tacccgccga gatcaccgtg ccttcctcga
     6481 tggcgaccac ccgcgatccg gccggggcat acagatccac gcctgcgtgc cggcggtcac
     6541 cgcggtcctc ccagaaagat ccctgatctc cggcggatgg caggatccga tcccggtccg
     6601 ggaggggcca gcatctcgcc ggcgagggat ggccttctcc cccggcacct gcacctcccg
     6661 atgaggagcg gtgcggaccc ttcctgtgcc ggggagacat ggaggtacct tctcgctccg
     6721 ggggaaaaag gatttttcgg gtttgccccg ggaaaaaatg ggtttcagga tgcaacggtc
     6781 acgggtattc tctgctgata ggtggccggg ctccggcggg atgtttcctg gtgcttctct
     6841 cccgggaccg cggccaccat ggcgaagcgg ctcccccccg agaggaccga gcggagcgcc
     6901 cggagggaga tggtgtacct ctccgccccg aagagctcga cattcaccgc cttaccggac
     6961 cgggagagcc cgcagagccc ctcgaccccg tcgaggtaga acctccagtt cttccggagc
     7021 ctctccaccc gggcctgggc gccggtggcc aggagatcct gcatgtccga gttccggaca
     7081 ccgaaccgca tgccccggat gtgcagggtg aggacctcct cctcgtttcc ctcaccgtcc
     7141 aggacccttg accggtccat gaggatgtat ccgacgttgt ccatcccgat ctcctctgat
     7201 cacccgtgtt gcccggagga gtatataaag tctcgatggg cggggggtga aagtgaaaat
     7261 cagatgagga cctttatccc ggcgatgatg agctgcaccc cgatggcggc gaggatcatc
     7321 ccgaggacc
//
LOCUS       DATGDI010000137         1940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_304059, whole genome shotgun sequence.
ACCESSION   DATGDI010000137 DATGDI010000000
VERSION     DATGDI010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304059"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VKO45_05155"
     CDS             complement(<1..410)
                     /locus_tag="VKO45_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion system protein E"
                     /protein_id="HMA05297.1"
                     /translation="MSTLTVQTNLPFKPAKTDEGRDCSANIEECSIYRMLPAHAREYM
                     KKSPHLLEYLHTYPVDKYGIPLFFSDLKRDLRTIKEPNLIYPANEETFIHIFPDPVDA
                     RNWYIPIEPSFLRSVNDLIPAVEVKLVSLLDALE"
     gene            complement(414..1160)
                     /locus_tag="VKO45_05160"
     CDS             complement(414..1160)
                     /locus_tag="VKO45_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HMA05298.1"
                     /translation="MPPDEKQELSDKTGEKSKAVLITGKADIDKKIDDGIPLRSLTLI
                     EGEHDSGKSVLIQQIVWGAMKQGLCADIFSSETTPRGFLPQMEAMSLDISDYYAWGYL
                     RLFPITAPGLDLEKENMEELLQRLVGYIRESPAEVIIIDSLTLFTEYTTNEVIIDFFT
                     SCKSLCDKGKTILVSLHSYAFDEDTLVRIRSVCDALLTLKRALIGDKYLMVLEVVKIR
                     GAHKTRENIVSFEVVPGYGMKIIPISVAKV"
     gene            complement(1170..1646)
                     /locus_tag="VKO45_05165"
     CDS             complement(1170..1646)
                     /locus_tag="VKO45_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaF"
                     /protein_id="HMA05299.1"
                     /translation="MGAGTIIATAIAITLLIITGYVLIGGTLSTARMVTLAQQAAAEQ
                     ESQRLHTRIEIFSVITDNASARTYIEVNNTGSEVVGSFDHMEVYLLQEGIPYVYTNAS
                     GNWTYTIQPDLVHPNLLDPDEVANITVSYDSLRGNATWVKVTTSNGISDSKYSMFI"
     gene            complement(1649..>1940)
                     /locus_tag="VKO45_05170"
     CDS             complement(1649..>1940)
                     /locus_tag="VKO45_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HMA05300.1"
                     /translation="NVGSSRISLADLNMSTIFIGAPGGYETVSRVGTFPPSSTGQWAY
                     EILEDTANGYWDPMETLHISTKSDKIPASGNVVYFQIVLADGTKRSEEFTVS"
BASE COUNT          374 a          502 c          619 g          445 t
ORIGIN      
        1 ttctccaggg catcgaggag gctcacgagc tttacctcca ccgccgggat gagatcgttc
       61 accgagcgga ggaaggaggg ctcgatgggg atgtaccagt tgcgggcatc gacgggatcg
      121 gggaagatgt ggatgaaggt ctcctcgttg gccgggtaga tgaggttggg ctccttgatg
      181 gtcctcagat cccgcttcag gtcggagaag aagaggggaa tcccgtactt gtccaccggg
      241 taggtgtgga ggtattccag gaggtggggg ctcttcttca tgtactcccg cgcatgggcc
      301 gggagcatcc ggtaaatgga gcactcctcg atgtttgccg agcagtcgcg accctcatcg
      361 gtcttcgccg gcttgaaggg caggttcgtc tggacggtga gggtggacat ggatcacacc
      421 ttcgcgacgg agatgggaat gatcttcatg ccgtacccgg ggacgacctc gaaggaaacg
      481 atgttctctc tcgtcttgtg ggcgccgcgg atcttgacca cctcgaggac catgaggtac
      541 ttgtccccga tgagggcccg cttgagggtg aggagggcgt cgcagaccga gcggatccgg
      601 accagggtgt cctcgtcgaa ggcataggag tggagggaca cgaggatggt cttccccttg
      661 tcgcagaggg acttgcagct cgtgaagaaa tcgatgatga cctcgttcgt ggtgtactcg
      721 gtgaagaggg tcagggagtc gatgatgatg acctcggccg gggactcgcg gatgtagccg
      781 accaggcgct gcaggagctc ctccatgttc tccttctcga ggtcgaggcc gggggcggtg
      841 atgggaaaga gccggaggta gccccaggca tagtagtccg agatatcgag actcatggcc
      901 tccatctggg ggaggaatcc cctcggcgtg gtctcgctcg agaagatatc ggcgcagagc
      961 ccctgcttca tcgctcccca gacgatctgc tggatgagga ccgatttccc gctgtcatgc
     1021 tccccctcga tgagggtgag ggaccggagg gggatgccgt cgtcgatctt cttatcgatg
     1081 tcggccttcc ccgtgatgag gacggccttg gacttctcgc ctgttttatc tgaaagttcc
     1141 tgtttctcat ctggcggcat ggatctctcc tagataaaca tggaatattt ggaatcggat
     1201 atcccgttcg aggtggtcac ctttacccac gtggcgtttc cccgtaaaga gtcataagag
     1261 acggttatat tcgctacctc gtccggatca aggaggttgg ggtgaaccag gtccggctga
     1321 atcgtgtacg tccagtttcc tgaagcgttc gtgtatacat aagggatccc ctcctgcagg
     1381 aggtatacct ccatatgatc gaaagatcct accacttcac ttccggtatt atttacctcg
     1441 atgtacgtcc tggctgaggc attatccgtt atgacactga aaatctcgat ccgggtatga
     1501 agcctttggc tctcctgttc tgcggcggct tgctgggcca gggtcaccat gcgggctgtg
     1561 gagagagtac cgccgatcag tacataaccg gtgataatga ggagggtaat ggcgatggcg
     1621 gtcgcgatga tggttcctgc gcccatcgtc agctcaccgt gaactcttcc gatcgtttcg
     1681 ttccgtcggc gagcacgatc tggaagtaga caacattacc gctcgcgggg atcttatcac
     1741 tcttcgtgga gatgtgaagg gtctccatgg ggtcccagta gccatttgca gtgtcttcaa
     1801 ggatctcata ggcccactgg ccggtagagg agggcgggaa ggtgccgacc cttgatacag
     1861 tctcataccc tcccggtgct ccgatgaaga tggtggacat gttcaggtcg gccaggctga
     1921 tccgactcga tccgacattc
//
LOCUS       DATGDI010000138         9300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_333550, whole genome shotgun sequence.
ACCESSION   DATGDI010000138 DATGDI010000000
VERSION     DATGDI010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9300
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333550"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VKO45_05175"
     CDS             complement(<1..329)
                     /locus_tag="VKO45_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA05301.1"
                     /translation="MPASPSYLALAATGELQDRIARACELLTDCGVCPRHCGVDRTAG
                     EIGYCRGGFLPRVSSAGPHFGEEPPLVGQSGSGTIFLTGCNMRCAFCQNYGISQCYHG
                     EEVSCGHL"
     gene            complement(414..1034)
                     /locus_tag="VKO45_05180"
     CDS             complement(414..1034)
                     /locus_tag="VKO45_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HMA05302.1"
                     /translation="MLESDEYSRILDILGNRNRRRIISLLQQKPCFVTELSERLTLSP
                     KALIEHLALMEQMEILGSYLDDRRRKYYYLTREISVRVSFAEKEGAARSFAGKDPAWR
                     RTLTLLRRMIRAREGLITNLEQLDRDIQQKVGDLARQGGDLITSEEEMDIILALTHYD
                     LTLAELRDFMDMPAEVVGMILGTLMEKRIVEKKGAKYSLRGIGDSG"
     gene            complement(1082..2554)
                     /gene="guaB"
                     /locus_tag="VKO45_05185"
     CDS             complement(1082..2554)
                     /gene="guaB"
                     /locus_tag="VKO45_05185"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HMA05303.1"
                     /translation="MFLEKLDMPLAITFDDVLLEPAASSTEPDQADVRSRFSRSVPLN
                     IPLVSAAMDTVTESSVAIALAREGGIGVIHRNMSAELETQEVRAVKHAEDLIQRNVIT
                     VGTDATVADVERLMNEHQISGVPVMKGNRICGIVSRRDIRGIAKKHGGENIQQFMTRK
                     PITATESTTMEEALETMYNRKVERLPVVGKKGTLVGIITMQDILHKHQYPQANRDRQG
                     NLRVAAAVGPFDFKRAGMLADAGADAIVVDCAHGHNLRVVEGVKEIKGSLKIDVIAGN
                     IATREAAGALAGHVDGLKVGIGPGSICTTRIVAGVGVPQITAVADVAEVARGHDVPVI
                     ADGGIRYSGDIAKAIAAGADSVMIGSLFAGTDESPGRIIVMKGRRYKQYRGMGSLGVM
                     SSGESSDRYFQKKSREFGKTKFVPEGVEGVTPYAGPIAEVTYMLVGGLKQAMGYVGAA
                     TVAEIQEKARFRRITPAGHLESHPHNILITDEAPNYRIFE"
     gene            complement(2560..3246)
                     /locus_tag="VKO45_05190"
     CDS             complement(2560..3246)
                     /locus_tag="VKO45_05190"
                     /EC_number="4.2.3.153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HMA05304.1"
                     /translation="MFLLVSPCSIEEASRSLAADIIDVKRPSEGSLGANFPWVIRAVK
                     GMTAKPVSAAIGDFDYRPGGAALAAYGAACAGADYVKVGLKFSGTEQARELIGAVVRA
                     VKEEFPGKSVVIAAYADHARLGTIPPFEAACLAAEGGADVAMIDTGLKDGRSTFEFMD
                     EATLTDYTLANRGLGLLTALAGSLRFEDIGALKRINPDIIGVRGMVCGGDRSAAIEPA
                     LVEKALRMIG"
     gene            complement(3438..3776)
                     /locus_tag="VKO45_05195"
     CDS             complement(3438..3776)
                     /locus_tag="VKO45_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05305.1"
                     /translation="MPAMSGTEITSLVVELVCGVAIGAILMNVLQDAINPIIVFVIII
                     ATIIAILDFADYMPAFSIAYFTGYVVEFLHSFAGIFDGLEIPVLVIALIGIGAVVAMK
                     ARRKRDRKTL"
     gene            3925..4593
                     /locus_tag="VKO45_05200"
     CDS             3925..4593
                     /locus_tag="VKO45_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HMA05306.1"
                     /translation="MDLVLALDLMKGRVVHGARGERSTYRPLLLPGIPSSRPEEIVSA
                     ISPRFLYIADLDRITRSGNHDDAIRACSRLVGRSYVDRGIRSPADYLEGENLVNVVGT
                     ETGGRDLAGYPGGFLSVDVKDGKVIPGGEDPRVILAMAGGWAFEGCILLNLGRVGTGS
                     GLSPEIGEFRDICKRRLFYGGGVASVADLEQLREAGFDGAIVATGVHRGTIPLEWVRR
                     GSLC"
     gene            4587..5204
                     /gene="tmk"
                     /locus_tag="VKO45_05205"
     CDS             4587..5204
                     /gene="tmk"
                     /locus_tag="VKO45_05205"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HMA05307.1"
                     /translation="MLITLEGIDGSGKSTLVAALRETLSDLDPVFTREPGSTWVGDAV
                     RRAIAERVDPVTEALLFVADHAAHLETVVRPALDLGRLVISDRYTDSRYAYQGALLEG
                     RLPDPMAWLRALHDGWTVVPDRTFLLLIPPDEAIRRLPADKRREHFEDGPLLDRVQKN
                     YLTLAESDPARFVLVDALLPAGEIHRFVESGIRELARRPRSRRRR"
     gene            complement(5161..7752)
                     /locus_tag="VKO45_05210"
     CDS             complement(5161..7752)
                     /locus_tag="VKO45_05210"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HMA05308.1"
                     /translation="MAPSPEIPKHYDFAEAEERWQRTWRDEENYFDRHSDRPRYIIDT
                     PPPYPTGNFHIGNALNWCYIDFIARYKRMQGYNVMFPQGWDCHGLPTEVKVEEIHGIT
                     KNDVSREEFRRMCRELTEKNIGLMRATLRRLGFSVDWSNEYITMRPEYFGKTQKSFLR
                     MFRDGYIYQAEHPVNFCIRCETAIAFAEVTYQERETTLTTFLFDGVEIATTRPELLAA
                     CVAVAVHPEDERFTRLHGRELTVPFFGQKVPVLQDEAVDPTFGTGAVMICTFGDKQDV
                     HWWKQYNLPLRKAIDRKGMMTGIAGKYAGMTVGDARKAIVADMKKEGLLRGEKRITQR
                     VGSCWRCKTPIEILSERQWFIKVKHDEILKAAREVTWIPAHMRTRLENWVAQMEWDWC
                     ISRQRIFATPIPVWFCEACGEMILPSEEDIPVDPTLTLPKTPCPKCGGRDFTPEEDVL
                     DTWMDSSISVLHVTGWDGKTTPPLFPAQLRPQGHDIIRTWACYTILRSVALTGQKPWD
                     GILVNGMVLGEDGFKMSKSRGNIIQPEEIIGKYGADSFRQWSASGAATGSDIMFNWND
                     VVAASRLQTKLWNIVRFILPKIGDRVYDPAEATVLADRWLLTRLSETVSAVTAAMETY
                     DFDQALKAIREFAWDVLADQYIELVKGRLYSEGRDRESAAAALSITLDALLRMLAPFL
                     PHFSEECYHHLRGRSVHHESWVTFRFTNDSAREKGDLLARVVSGLRTYKHDHGLALNA
                     PLGRLTIYAGTALEEGGDAARALNAGVVWMAGEPRLEKRVSDVKFNMGVVGPALKGKA
                     RGFMEAVRRLPPGSLENPPSAILLGGEEVPVPAGAFSPAFSYAIGGKDVDVVTVGDVI
                     VAVERAP"
     gene            8058..9278
                     /locus_tag="VKO45_05215"
     CDS             8058..9278
                     /locus_tag="VKO45_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HMA05309.1"
                     /translation="MRSREGIMQYTGILSALALTAGCILAAPFIEPPFILSILVIVFS
                     GILYLIRGTRSISISIVILAILYGLGWLPLLIFAATLGIVIVGEIAYRSNPGGHHRYL
                     TYFAASAAGGALAMLSLGHRDPLLLILGVVVAVLLKSALRERNDVLMVEGLGVAMTMY
                     LFEDLNYQVPAQILLLAIVVAFSFGYFAYRFRVADVSGLFSGTLVGIILIVFADVRWF
                     LVMIAFLAMGSGATRYRFEAKAAAGIAESHGGVRGYVNVFANGIVGVAAAVLYGLSGG
                     NPLPLALFLGSVATAAADTMGGEIGMTSHDPVLITTLEPVPKGTNGGITMLGEASALC
                     ASVAIAASALLLGIGSSRMLLFAVLAGFAGSNVDSLVGALLENRGVIGNAGTNLIATL
                     TGGLIAAGLFFLFP"
BASE COUNT         1488 a         3060 c         3028 g         1724 t
ORIGIN      
        1 agatgtccgc aggagacctc ttcgccgtgg tagcactggc tgatcccgta gttctggcag
       61 aaagcgcacc gcatgttgca gccggtgagg aagatggtcc cggagccgga ttgcccgacg
      121 agaggcggct cctcgccgaa gtggggaccg gcactcgaaa ccctcgggag gaaacctccc
      181 cggcagtagc cgatttctcc ggccgtccgg tccacgccgc agtgccgggg acagaccccg
      241 cagtctgtga gaagctcgca tgcccgggcg atgcgatcct ggagctcacc tgtcgctgcc
      301 agggcgaggt aagaagggga cgcgggcatc agcaacggat tacctcacgg ggaaataaag
      361 ataccatgag gcggggcacg gcctacatgg ggtgcttgaa atcacccggg cggttatccg
      421 gaatccccga tgccccggag ggagtacttg gcgcccttct tctccacgat ccgcttttcc
      481 atgagggttc cgaggatcat gcccacgacc tcggccggca tatccatgaa gtcgcggagc
      541 tcggcgaggg tgaggtcata gtgggtgagg gcgaggatga tgtccatctc ctcctcgctc
      601 gtgatcaggt ccccgccctg ccgggccagg tccccgacct tctgctggat gtcccggtcc
      661 agctgctcga ggttggtgat gagcccctcc ctcgcccgta tcatgcgccg gagcagggtg
      721 agggtcctcc tccaggctgg gtcctttccg gcgaaagacc gggctgcacc ctccttctcc
      781 gcgaaactca ccctgaccga gatctcccgg gtcaggtagt agtacttcct ccggcggtcg
      841 tccaggtagc tccccaggat ctccatctgc tccatgaggg cgaggtgctc gatgagggcc
      901 ttcggggaga gggtgaggcg ctccgagagc tcggtgacga agcagggctt ctgctgcagg
      961 aggctgatga tccgcctccg gttccggttt cccaggatgt ccaggatccg ggagtactcg
     1021 tcgctctcga gcatccttaa ctaaatgtta gggtcttctc tcccaaaaaa gatgatcccg
     1081 gtcactcgaa gatccggtag ttcggggcct cgtcggtgat gaggatgttg tggggatggc
     1141 tctcgaggtg gcccgcgggg gtgatgcgcc ggaaccgggc tttctcctgg atctcggcga
     1201 cggtcgccgc cccgacgtac cccatggcct gcttgaggcc gccgacgagc atgtaggtga
     1261 cctcggcgat gggcccggca tagggggtga ccccctccac gccctcgggg acgaactttg
     1321 tcttcccgaa ctcccgggac ttcttctgga agtaccggtc gctcgactcc ccgctgctca
     1381 tcaccccgag agaacccatg ccccggtact gcttgtagcg ccggcccttc atcacgatga
     1441 tccttcccgg cgattcgtcg gtcccggcga agagggagcc gatcatgacg ctgtcggcac
     1501 cggccgcgat ggccttcgcg atgtccccgg agtaccggat acccccgtcg gcgatgaccg
     1561 ggacgtcgtg gccccgggcg acctcggcca catcggcgac ggcggtgatc tggggcaccc
     1621 cgacgccggc cacgatccgg gtcgtgcaga tggagcccgg gccgatcccg accttcaggc
     1681 cgtccacgtg gcccgcgaga gccccggccg cctcccgggt cgcgatgttc ccggcgatca
     1741 cgtcgatctt caggctcccc ttgatctcct tcaccccctc caccacccgc aggttgtggc
     1801 catgggcaca gtccaccacg atggcatcgg ccccggcatc ggcgagcatc cccgcccgct
     1861 tgaagtcaaa gggccccacc gcggccgcga cccggaggtt cccctgccgg tcccggttcg
     1921 cctggggata ctggtgcttg tggaggatgt cctgcatcgt gatgatcccg acgagggtgc
     1981 ccttttttcc gacgaccggg agccgctcca ccttccggtt gtacatggtt tccagggcct
     2041 cctccatcgt ggtgctctcg gttgcggtga tgggcttcct cgtcatgaac tgctggatgt
     2101 tctccccgcc gtgcttcttc gcgatccccc ggatgtcgcg acggctcacg atcccgcaga
     2161 tccggttccc cttcatcacg ggcacgccgc tgatctggtg ctcgttcatg aggcgttcga
     2221 cgtcggcgac ggtcgcatcg gtccctaccg tgatgacatt cctctggatg aggtcctcgg
     2281 cgtgcttgac cgcccggacc tcctgggtct ccagttcggc ggacatgttc cggtggatga
     2341 ccccgatccc accctccctc gcgagggcga tggccaccga tgattcggtc accgtgtcca
     2401 tggcggccga aacgaggggg atgttcaggg ggacggatct cgagaaccgg ctgcggacat
     2461 ccgcctggtc cggctcggtg gaggaggcgg cgggctcgag gagcacgtca tcgaaggtga
     2521 tggcgagggg catgtcgagt ttttcgagga acatggggat cacccgatca tcctgagcgc
     2581 cttttccacg agggcgggct cgatggcggc cgaccggtcg cccccgcaga ccatcccccg
     2641 gaccccgatg atgtcggggt tgatcctctt taacgccccg atgtcctcga accggaggga
     2701 cccggcgagg gcggtgagga gccccagccc ccggttcgcg agggtgtaat ccgtgagggt
     2761 cgcctcgtcc atgaactcaa aggtgctccg gccgtccttc aggccggtgt cgatcatggc
     2821 cacgtcggca cccccctcgg cggcgaggca ggccgcctcg aaggggggga tggtcccgag
     2881 ccgggcatgg tcggcgtagg cggcgatgac cacggacttc ccggggaact cctccttcac
     2941 cgccctcacc accgccccga tgagctcccg ggcctgctcc gtcccgctga acttgaggcc
     3001 gaccttcacg taatcggccc cggcgcaggc cgcgccatag gcggcgaggg ccgcgccccc
     3061 gggccggtag tcgaaatcac cgatggcggc gctgaccggt tttgcggtca tgcccttcac
     3121 ggcccggatc acccagggga agttcgcgcc gagggatccc tcggagggcc tcttcacatc
     3181 gatgatgtca gcggcaagcg atcgggatgc ctcctcgata ctgcacgggc tgacaagaag
     3241 gaacatggaa gatggtggta cgtcgtggtt aatagcggtt gctgtttccc cgatggcaac
     3301 ctccgggcgg gtcccgggga agccccggac cgcgaaggga gggtgccccg gggaagctgg
     3361 gggccggccc cgagccgggc aggataaagg gagttcccgg cccggcgtga aaagtctccc
     3421 cggcaccggc cgggacgcta gagggtcttc cggtccctct ttcgcctggc cttcatcgcc
     3481 accaccgccc ctatcccgat gagggcgatg acgaggaccg ggatctcgag gccatcgaag
     3541 atcccggcga aggagtggag aaattccacg acatagcccg tgaagtaggc gatggagaag
     3601 gccggcatgt agtcggcgaa gtcgaggatg gcaatgatgg tggcgatgat gatgacgaag
     3661 acgatgatgg ggttgatggc atcctggagg acgttcatca ggatggcccc gatggcgacc
     3721 ccgcagacga gttccacgac gagggaggtg atctctgttc ccgacatggc aggcatggtc
     3781 aaaccgggta tcgacctgct ctcttatatc gtttctcacg gcaggcccta cctcctatcg
     3841 acgggctctc ccttccgatc ccggaggccg cgctcatcgg cgggataccc atcgcttata
     3901 tcctttcaac atgcacctct tcccatggac ctcgtcctcg ccctggacct gatgaaagga
     3961 agggtcgtcc acggcgcccg tggggaacgg tcgacctacc ggccgctcct tcttccggga
     4021 atcccatcat cccgccccga ggagatcgtc tctgccatct ccccgaggtt cctctacatt
     4081 gcggacctgg accggatcac gaggtcaggg aaccatgacg atgccatcag ggcgtgctcc
     4141 cgcctggtcg gcaggtccta cgtcgaccgg gggatccggt cccctgcgga ttacctggag
     4201 ggggagaacc tggtgaacgt cgtcgggaca gagaccgggg gccgggacct cgcgggctac
     4261 cccggggggt tcctgtcggt ggacgtgaag gacgggaagg tcatcccggg cggggaggac
     4321 ccgagggtga tcctcgcgat ggccgggggc tgggctttcg agggatgcat tctcctcaac
     4381 ctcgggaggg tgggaaccgg ttccgggctc tcccctgaaa tcggggaatt cagggatatc
     4441 tgcaaaagga ggctcttcta cgggggaggg gtggcgtcgg tcgcggacct ggagcagctc
     4501 agggaggcag gcttcgacgg ggccatcgtg gccaccgggg tgcaccgggg aaccatcccc
     4561 ctcgaatggg taaggagggg atccctctgc tgatcaccct cgaaggaatc gacgggagcg
     4621 ggaagagcac cctcgtcgcg gccctccgcg aaactctttc ggacctggac ccggtcttta
     4681 cccgggagcc gggctccacc tgggtggggg atgcggtccg gcgggccatc gcggagcggg
     4741 tggatccggt gaccgaggcc ctcctcttcg tcgcggacca cgcggcccac ctggagacgg
     4801 tcgtgcgacc ggccctcgat ctgggaaggc tcgtcatctc ggaccggtac accgacagca
     4861 ggtacgccta ccagggcgcc ctgctggagg gaaggctccc ggacccgatg gcatggctac
     4921 gggccctcca cgatggctgg actgttgtcc cggaccggac cttcctcctc ctcatccccc
     4981 ccgacgaggc tatcaggcgc ctccccgcgg ataagcgcag ggagcacttc gaggacggtc
     5041 ccctcctcga tagagtccag aagaactacc tcaccctcgc cgagtccgat ccggcgcggt
     5101 tcgtcctcgt ggacgctctc ctcccggcgg gggaaatcca ccggttcgtg gagtcaggga
     5161 tcagggagct cgctcgacgg ccacgatcac gtcgccgacg gtgaccacgt ccacgtcctt
     5221 cccgccgatg gcgtaggaga aggccgggga aaaagcgccg gccgggaccg ggacctcctc
     5281 cccgccgagg aggatggcgg acgggggatt ttcgagggat ccggggggaa gccggcggac
     5341 tgcctccatg aacccgcggg ccttcccctt gagggcgggg ccgacgaccc ccatgttgaa
     5401 cttcacatcg ctaacccgct tctcgagcct cggttccccg gccatccaca cgaccccggc
     5461 attgagggcc cgggccgcgt ctcctccctc ctcgagggct gttcccgcgt agatggtcag
     5521 ccgcccgagg ggtgcgttca gggccagccc gtgatcgtgc ttgtacgtcc tgaggccgga
     5581 gaccacccgg gccaggagat cccccttctc ccgggcggaa tcgttggtga accggaaggt
     5641 aacccaggat tcgtggtgga ccgacctccc cctcaggtgg tgataacatt cctcggagaa
     5701 gtgggggagg aagggggcga gcatccggag gagggcatcg agggtaatgg agagggccgc
     5761 ggcggcactc tcccggtccc ttccctccga gtagagcctc cccttgacga gctcgatgta
     5821 ctggtcggcg aggacatccc atgcaaactc gcggatcgcc ttcagtgcct ggtcgaagtc
     5881 gtaggtctcc atggcagcgg tgaccgcgga tacggtctcc gacagccggg tgaggagcca
     5941 gcggtcggcg aggacagtcg cctccgccgg atcgtacaca cggtccccaa ttttcgggag
     6001 gatgaaccgg acgatgttcc agagcttcgt ctggagcctg gatgcggcga cgacatcgtt
     6061 ccagttgaac atgatgtccg aacccgtcgc cgcgcccgag gccgaccact gccggaagga
     6121 gtctgccccg tacttgccga tgatctcctc gggctggatg atgttccccc ggctcttgga
     6181 catcttgaag ccgtcctcgc cgaggaccat gccgttcacg aggatgccgt cccagggctt
     6241 ctgccccgtg agggccaccg agcggaggat ggtgtaacag gcccaggtcc ggatgatgtc
     6301 atgcccctgg ggccggagct gggcgggaaa gaggggcggt gtcgtctttc cgtcccagcc
     6361 ggtcacatgg aggacggaga tggaggagtc catccaggtg tcgaggacat cctcctcggg
     6421 ggtgaagtcc ctccccccgc acttcgggca gggggttttc ggcagggtga gggtcgggtc
     6481 cacgggaatg tcctcctccg acgggaggat catctcgcca caggcttcgc agaaccagac
     6541 cgggatgggg gttgcaaaga tccgctgcct cgagatgcac cagtcccact ccatctgggc
     6601 gacccagttc tcgagacggg tccgcatgtg cgccggtatc caggtcacct ccctcgcggc
     6661 cttcaggatc tcgtcgtgct tcaccttgat gaaccattgg cgctcggaga ggatctcgat
     6721 gggggtcttg caccgccagc aactccccac ccgctgcgtg atccgcttct cgccccggag
     6781 gagcccctcc ttcttcatgt cggcgacgat ggccttccgg gcatccccca ccgtcatccc
     6841 ggcgtacttc ccggcgatcc cggtcatcat ccccttccgg tcgatggcct tccgcagggg
     6901 gagattgtac tgcttccacc agtggacgtc ctgcttgtcc ccgaaggtgc agatcatgac
     6961 cgcgcccgtc ccgaaggtcg ggtcgaccgc ctcgtcctgg agcaccggga ccttctggcc
     7021 gaagaagggg acggtgagct ccctcccgtg gagccgggtg aagcgctcgt cctccgggtg
     7081 gaccgccacc gcgacgcagg ccgcaagcag ctcgggcctg gtggtcgcga tctcgacccc
     7141 gtcgaagagg aaggtcgtga gggtggtctc ccgctcctgg taggtcacct cggcaaaggc
     7201 gatggcggtc tcgcaccgga tgcagaagtt caccgggtgc tcggcctggt agatgtaccc
     7261 gtcgcgaaac atccggagga aggatttctg ggtcttgccg aagtactcgg gacgcatcgt
     7321 gatatactcg ttcgaccagt cgaccgagaa tccgagccgg cgcagggtgg ccctcatgag
     7381 cccgatgttt ttctccgtga gctcccggca catccgccgg aattcctccc gggagacatc
     7441 gttcttcgtg atgccgtgga tctcctccac cttcacctcg gtgggaaggc cgtggcagtc
     7501 ccacccctgg gggaacatga cgttgtatcc ctgcatccgc ttgtaccggg cgatgaagtc
     7561 gatgtagcac cagttcaggg cgttcccgat gtggaagttc cccgtcgggt agggaggggg
     7621 cgtgtcgatg atgtaccgag gcctgtcgga gtgccggtca aagtaattct cctcgtcccg
     7681 ccaggtccgc tgccaccgtt cctctgcctc agcgaagtca tagtgcttgg gaatttcggg
     7741 tgagggggcc aaattttccg tccaggcgcg gttgtgcctt ctcatttttg atcctcaagg
     7801 aaaaaaatac ctttcattcc tgaccggtac tggcgcgggg gagagcatgg ccaaccttca
     7861 cgacttacgt tcatcatccc cgcacggagg atccgaagca cgatctcctc ccgtgaatcc
     7921 cttacgcggc cgtgacaggc gggcctcggc aggaaggcgg tcaccaaaaa ggattatcca
     7981 ttcgggttca gatgtttcag ggaatgaaga ggcccgtggc ggaccccggc cctgaggcgg
     8041 ggagcccacc cggaaggatg aggtcccgcg aggggatcat gcagtacacc gggatcctct
     8101 cggcccttgc cctgacggcg ggatgcatcc tcgccgctcc tttcatcgag ccccccttca
     8161 tcctctctat cctcgtcatc gtcttctccg ggatcctgta cctcatccgg gggacccggt
     8221 ctatttccat ctccatcgtc atcctcgcca tcctctacgg cctcgggtgg cttcccctcc
     8281 tcatcttcgc cgccaccctt gggattgtca tcgtcgggga gatcgcctac cggtcaaacc
     8341 cggggggcca ccaccggtac ctcacctact tcgccgcctc agccgccggg ggggctctcg
     8401 cgatgctctc cctcgggcac cgggatcccc tcctcctcat cctgggcgtg gtggtcgcgg
     8461 tcctcctcaa gtctgccctc cgcgagagga acgacgtgct catggtggag ggcctcgggg
     8521 tagccatgac catgtacctc ttcgaggacc tgaactacca ggtcccggcc cagatcctcc
     8581 tcctcgccat cgtcgtcgcc ttctccttcg ggtacttcgc ctaccggttc cgggtcgcgg
     8641 acgtgagcgg actcttctcg ggaaccctcg tcgggatcat cctcatcgtc ttcgccgacg
     8701 tgcggtggtt cctcgtcatg atcgccttcc tggccatggg atcgggtgcc acccggtacc
     8761 ggttcgaggc gaaggcggcg gcggggatcg cggaatccca cgggggcgtc cgggggtacg
     8821 tgaacgtctt tgcaaacggc atcgtcgggg tcgcggccgc ggttctctac ggcctctcgg
     8881 gtgggaaccc gctccccctg gccctcttcc tcggcagcgt ggccaccgcg gcggccgata
     8941 ccatgggggg cgagataggg atgacctccc atgacccggt gctcatcacc accctcgagc
     9001 ccgttccgaa ggggacaaac ggggggatca ccatgctcgg cgaggcctcg gccctctgcg
     9061 catcggtggc catcgcggcg agcgccctcc tcctcgggat cggatcctcc cggatgctcc
     9121 tctttgcggt cctcgccggg ttcgccggtt cgaacgtcga cagccttgtg ggggcgctcc
     9181 tcgaaaacag gggggtgatc gggaacgcgg ggacgaatct catcgcgacc ctgacgggag
     9241 gccttatcgc ggccggcctc ttcttccttt tcccgtaaag gcggtaccta gtagcggtac
//
LOCUS       DATGDI010000139         1877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_72252, whole genome shotgun sequence.
ACCESSION   DATGDI010000139 DATGDI010000000
VERSION     DATGDI010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72252"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VKO45_05220"
     CDS             complement(<1..359)
                     /locus_tag="VKO45_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HMA05310.1"
                     /translation="MIGDFINKYYIAGIVQGQPYNIVDTATYAIVLVISVYLVYRWLR
                     RSGVALDGPFILATIPFVVLGGLLRVVEDTGMIGPPAKYLLITPLIFFVVFFITAAAL
                     VLSRTLEKHRLVPRFRT"
     gene            358..1449
                     /locus_tag="VKO45_05225"
     CDS             358..1449
                     /locus_tag="VKO45_05225"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-1-phosphate
                     dehydrogenase"
                     /protein_id="HMA05311.1"
                     /translation="MINQNRAQGMKVLRNKGFDKSKWMQLPRDVLVGHGVLGQLPRVC
                     EDLAVGKSALVLTGRHTLGVAGKKVIAMLSRRYDTRTFLAEEISPAVIREAEKEARKA
                     EILVAVGGGKIIDTAKVASFNTDRQFISVPTAASHDGIASASASVPTPEGNLSLQAHP
                     PVAIVADTGIIASAPHRLLASGCADIISNESAVLDWELSHRLKGEPISEYASTLSRMT
                     AEGMIRNADLVKPNSEETAWMVVKALVASGVAMSIAGSSRPASGGEHKFSHALDRIAP
                     GKGLHGEKCGIGTLITLFLHGGDWRGAREALKKIGAPTSPAEIGIDDATAVEALLLAS
                     TIRPDRFTILDTGITRESAETLIRMLSKD"
     gene            1456..>1877
                     /locus_tag="VKO45_05230"
     CDS             1456..>1877
                     /locus_tag="VKO45_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HMA05312.1"
                     /translation="MPEAKPKVTLIGRLLARPGLEFIFEGELSDCEGCRVRKACNNLQ
                     PGRKYRIVSVRTTAPHDCSIHEGGTCAVEVVESPIVALVNADMAIMNSRITFEVSCNK
                     EECKSFELCRPDGVIDGERYLVGEILGNAPDICEKGRAL"
BASE COUNT          370 a          604 c          623 g          280 t
ORIGIN      
        1 aaggtacgga accggggaac gagcctgtgc ttctcgaggg tccgggagag gacgagagcc
       61 gcggctgtga tgaagaaaac gacgaagaag atgagggggg tgatgagcag gtacttcgcg
      121 ggaggcccga tcatccccgt gtcctccacc acccggagga gcccgcccag gacgacgaag
      181 gggatggtgg caaggatgaa cggcccgtcc agggccaccc ccgagcggcg gagccagcga
      241 tatacgaggt atacggaaat gacgagaacg atggcatagg tggcggtatc cacgatgtta
      301 tagggctggc cctggacaat gccagcgatg taatatttat tgatgaaatc cccaatcatg
      361 atcaatcaga accgggcgca gggtatgaaa gtactgagga ataaaggttt tgacaagtcc
      421 aaatggatgc agctcccccg ggacgtcctc gtcggccacg gcgtcctcgg gcagctcccc
      481 cgggtctgcg aggacctcgc ggtggggaaa tccgcccttg tcctcacggg gaggcacacc
      541 ctgggggtgg ccgggaaaaa ggtcatcgcg atgctctcca ggaggtacga cacccgcacc
      601 ttccttgccg aagagatcag cccggccgtg atccgggagg ccgagaagga ggcccgcaag
      661 gcggagatcc ttgtcgccgt cgggggcggg aagatcatcg acacggcgaa ggtcgcctcc
      721 ttcaacaccg atcgccagtt catctccgtg ccaaccgcgg catcccacga cggaatcgcc
      781 tcggcctcgg cctcggtccc gaccccggag gggaacctct ccctccaggc ccacccgccg
      841 gtcgccatcg tcgccgacac cgggatcatc gcctcggccc cccaccggct cctcgcctcg
      901 gggtgcgccg acatcatctc caacgagtcc gcggtcctcg actgggagct ctcccaccgg
      961 ctcaaggggg agcccatctc cgaatacgcc tccaccctct cccggatgac cgccgagggg
     1021 atgatccgga acgcggacct cgtcaagccg aacagcgagg aaaccgcctg gatggtggtg
     1081 aaggccctcg tcgcctcggg agtggccatg tccatcgcgg gatcctcgcg gccggcgagc
     1141 ggcggggagc acaagttctc ccatgccctc gaccggatcg cgccggggaa ggggctccac
     1201 ggggagaagt gcgggatcgg gaccctcatc accctcttcc tccacggggg ggactggagg
     1261 ggggcccggg aggctctgaa aaagatcggg gccccgacct ccccggcgga gatcgggatc
     1321 gacgatgcga ccgctgtgga ggccctcctc ctcgcgtcga ccatccggcc cgatcgcttc
     1381 accatcctgg acacggggat cacccgggaa tcggcagaga ccttaatcag gatgctctcc
     1441 aaagactaga tcggtatgcc cgaggcaaaa cccaaggtga ccctcatcgg gaggctcctc
     1501 gcccggccgg gccttgaatt catcttcgag ggggagctct ccgactgtga gggctgccgg
     1561 gtcaggaagg cctgcaacaa cctccagccg gggaggaagt accgcatcgt ctcggtccga
     1621 accacggccc cccacgactg ctccatccac gagggaggga cctgcgcggt ggaggtggtg
     1681 gagtcgccca tcgtcgcgct cgtgaacgct gacatggcca tcatgaactc ccggatcacc
     1741 ttcgaggtct cctgcaacaa ggaggagtgc aagtccttcg agctctgccg gccggacggg
     1801 gtcatcgacg gcgagcggta cctggtgggg gagatcctgg ggaacgcgcc ggacatctgc
     1861 gagaagggcc gggccct
//
LOCUS       DATGDI010000140        26957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_188518, whole genome shotgun sequence.
ACCESSION   DATGDI010000140 DATGDI010000000
VERSION     DATGDI010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 26957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26957
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188518"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VKO45_05235"
     CDS             complement(<1..192)
                     /locus_tag="VKO45_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HMA05313.1"
                     /translation="MTIEDRLRDLKEKIDGIVPAGISVSQVEFEGPELVIYTEDPKKF
                     ADQADLIKILARDLRKRIVV"
     gene            complement(221..862)
                     /gene="psmB"
                     /locus_tag="VKO45_05240"
     CDS             complement(221..862)
                     /gene="psmB"
                     /locus_tag="VKO45_05240"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit beta"
                     /protein_id="HMA05314.1"
                     /translation="MQELSQETMKGTTTIGLVFKDGVILATERRATMGNLVASKKAKK
                     VYQIADRIGMTTAGGVGDAQQLARIITVECSLYQIRRGRPISVGASSTLLSNYLNQNR
                     YYPYYVQLLVGGVDETGPSVYSVDAMGGATREEEIVSTGSGSPMAYGVLEDRYRPDMG
                     EQEAVDLAVRALRAAMRRDIASGEDINVVVITKEKYEEFIVENGQKHPISASA"
     gene            complement(940..1797)
                     /locus_tag="VKO45_05245"
     CDS             complement(940..1797)
                     /locus_tag="VKO45_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA05315.1"
                     /translation="MTGTEVRDFMTPDVVSVEIPGNRDDVLKILKRTGISGVPVLKEG
                     KLVGIITRKDMLRKSEETQLGLLMTRNPATIAPDASLEEAARLIIEKKVRRLPVVEDK
                     ALVGILSVADLVTAIAQQKNRMEIKDRLTSTTFALWEETPLVLVGRVMEISGVDAIPI
                     LDGEGVLSGIISERDLIKHSSIEDSVETSDFSNGTDDDEWTWESIRDLHTLSFGVSKV
                     KLPARPVKTAMVTNVVSVPLNSEVGECALKMKRARVDQLPVVNSDKRLVGMLFDREII
                     RVLCDGGQS"
     gene            complement(1794..2228)
                     /locus_tag="VKO45_05250"
     CDS             complement(1794..2228)
                     /locus_tag="VKO45_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMA05316.1"
                     /translation="MPMFQSILAAIDGSKKSEAALDIAIGEAKVHGAALHVVYVIETG
                     LFSSIPMDNTWEVIYGLLENQGKEAHLAAAKRAQAQGVAITTHLKEGHAGNEIIKLAR
                     ETGADLIVLGSHGKSNLDRILLGSISSFVVSHSPVSTLVVRS"
     gene            complement(2231..3298)
                     /locus_tag="VKO45_05255"
     CDS             complement(2231..3298)
                     /locus_tag="VKO45_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMA05317.1"
                     /translation="MTQADFQVSGHAIVGRDLDLRPVRITVEEGVIREIAEISSPPPV
                     WICPALFNAHTHLGDTVGMDMPWSGPLEDLVTPPGGLKHRLLKTSPRRTLREGMRASI
                     GVMVRSGTAGFADFREGGEAGVRDLRAAAEGLPCRPVIFGRDGGQGIAEGLGIPSVRD
                     FSDAEAAVSAARREGKLVAFHAGERDPLDIDGALALEPDLLIHCTHAEDRHLREIADR
                     DIPVAICPRSNWILGVSSSRDHPPLERMLTFGVTLLLGTDNAMFVQPDLWAEMAFAST
                     VYPIHPREILAAAIRGSGRFLSGGFIEEGSPARFLVINPAGSNLSFSRDPVASLVRRT
                     GPSDIREKYLFSPDKYVTQPF"
     gene            3340..3498
                     /locus_tag="VKO45_05260"
     CDS             3340..3498
                     /locus_tag="VKO45_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HMA05318.1"
                     /translation="MAKKQGGRLISSAGLVNYYESTDRRALQISPYTVAVVAAAIGVA
                     IFILNILY"
     gene            3608..3874
                     /locus_tag="VKO45_05265"
     CDS             3608..3874
                     /locus_tag="VKO45_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1922 domain-containing protein"
                     /protein_id="HMA05319.1"
                     /translation="MYIVIRCPGCSTFTYVDRFQRHKLCHICGEVIDVRTAPAYLEVK
                     DYPDAEEVVNQLQSFLEREGRKDLSPQELRMLRAKYAQWLLSQT"
     gene            complement(3875..4024)
                     /locus_tag="VKO45_05270"
     CDS             complement(3875..4024)
                     /locus_tag="VKO45_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PriA"
                     /protein_id="HMA05320.1"
                     /translation="MLRHSCGYEANFHCKNCHLPLSHDERIGLYCKKCGRRITTVCPG
                     CGRPW"
     gene            4185..5204
                     /locus_tag="VKO45_05275"
     CDS             4185..5204
                     /locus_tag="VKO45_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HMA05321.1"
                     /translation="MTHHLRRKILLAILLLAIAAIFALFWHWLDMIPFGIAVAVVAAP
                     AKNRLERRLPGWLSALAIALVTLLLLSAGILVTIQAMQENLATNEEILGRIASGIEAL
                     SPQLTTAGIPEGTVHTAVAWIEGSVQALAGFWLSLSLESVLLTPRVGLFFLSLGLALW
                     KGEAVLRAVLPRIPPSWLSVYPNLAAVSVDTLYAVIVVHLLIVGLTFGLSLPFFWILG
                     YGHVLYLSLVTAFCELVPVLGASIPMVVLLLYSLALGDLRGFLLVFFIGYLVVALLPE
                     VTIRPILMGRRTLLSPVLMFASFIGGILLLGISGFLLGPLVAALGVSWYRLRMEGRTG
                     QTQPP"
     gene            5265..5349
                     /locus_tag="VKO45_05280"
     tRNA            5265..5349
                     /locus_tag="VKO45_05280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5301..5303,aa:Leu,seq:tag)
     gene            5387..6016
                     /locus_tag="VKO45_05285"
     CDS             5387..6016
                     /locus_tag="VKO45_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HMA05322.1"
                     /translation="MASPAPTLHQTALHLEYFTVGYNIVEAVASIVFGNLAGSIALIG
                     FGLDSIVESLSGLVLIWRLRRHGHISRDEEERVERRAQRFVALTFFILGAYVLYESGA
                     KLIFREAAQPSSAGIIIALLSILIMPMLAWRKQQVGMGIGSRALIADSKETLACAFLS
                     VGLLAGLGANQLFGFWQADPLAGLFISLFLFREGYEGWRESGDEKADQD"
     gene            complement(6174..7121)
                     /locus_tag="VKO45_05290"
     CDS             complement(6174..7121)
                     /locus_tag="VKO45_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HMA05323.1"
                     /translation="MEKSYAIQTLIFFLIVIPGAWLAWTLSGMNIMTTFWAILWIGLI
                     AIVIAASFKVADQWEKAVVLRLGKYFGLKGAGPFLIIPIVDRVAYWIDLRIITTTFMA
                     EKTLTKDTVPVDVEAVLFWKVVDSEKAALAVENYRTAISWASQTALREIIGKSMLADM
                     LENREKLDKELQHIIDLRTEQWGVNISSVEIRDILIPQELQNAMSMQAQAERERQARV
                     ILGDSEKQIAQKFEEAARTYQDNPTALHLRAMNMLFEGLKTGNPTLVIVPASVLSTMN
                     LGDTTGVVALAQNLENQKEKAKLAGQVEKEMGGGYKPPK"
     gene            7419..9824
                     /gene="kdpA"
                     /locus_tag="VKO45_05295"
     CDS             7419..9824
                     /gene="kdpA"
                     /locus_tag="VKO45_05295"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HMA05324.1"
                     /translation="MARSQLRPALVIFLLLTLLTGVLYPVLVTGIAQLVFPVQANGDL
                     MVHDGKVVGSSLIGQPFSSPVYFWGRPSATALVPYNASASGGSNLGPSNPVLLDAVQA
                     RVDALHAADPANTRLIPVDLVTASGSGLDPDISVAAAYYQVPRVARVRNLSEGEVQGL
                     VAGHIQGRQLGIFGEPRVNVLSLNLALDDLATRGPGSPAPDLVPVSQGSYNETPVPGM
                     RGSDWLQLIFFFAAGAALIIPLGGFMAKVLTGEPTFLSPLTGPIEGWLLRSSGVKPGQ
                     EMDWRMFGIALMVFSGICLIVPFLLQETQQLLPLNPSGLGPVPWDLSLNTAVSFATNT
                     NWQFYVPETTVSYLTQMAGLAVQNFLSAAKGITVLVALVLAFSRRSAQTVGNFWVLLV
                     RAVMILLPLSLVLALLLVSQGSVQTFGGPVTVPLLDPVRDASGNLVTTQLISVGPAAS
                     QIAIKMLGTNGGGFFNANSAHPFENPTPLSNFIENLSLMIIAAALCYSFGQMIGSRRK
                     GIALLMAMTILFLPLAGIAIWSELGGNPAFDPMGVDQSPFHSQPGGNMEGKEVRFGIV
                     PSALWSTATTSTSCGAVNSMHDSYMPLGGFVQMFAMQLGEVVYGGVGSGLYGMLVFVV
                     IAMFIAGLMVGRTPELYGKKIEPQEMKLATVIILIPIFVTLTGTAIAVLTGPGTATTL
                     NPGPHGFSEILYAFTSTPQNNGSAFAGLSANLFYTISTAIAMIIGRYAVALFTLALAG
                     SFAGKKIVPASEGTLQDHRPLFIIWLVFVVVIVGALSYLPALSLGPIAEYLIQIGRGA
                     IHA"
     gene            9817..11919
                     /gene="kdpB"
                     /locus_tag="VKO45_05300"
     CDS             9817..11919
                     /gene="kdpB"
                     /locus_tag="VKO45_05300"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HMA05325.1"
                     /translation="MPDPIRARGPDPAPPALYRRAILDAVIKLDPRTLSRNFVMLVVE
                     MGSLLTTLLWVQALLGHGEAPAGFIGAIAAWLWFTVLFANFAEALAEGRGKAQAEALR
                     RMRQDTLAKRLDPCLRRERKKEPGPSDFTVVSSSELRREDIFYVKAGDAIPVDGEVIE
                     GIASVNESAITGESAPVIRESGGERSAVTGGTTLLSDWLVVRVSANPGEGFLDHMIGL
                     VEGAKRQRTPNEVALNILLVGLTAVFIVVCASLRAFSVYSVRAAGGGVPVTLTVLVAL
                     LVCLMPTTIGGLLSAIGIAGMDRLIRRNVITTSGRAIEAAGDVDALLLDKTGTITLGN
                     RQAVALIPVDGTDITELAEVSQLASLADETPEGRSIVVLAKERYGLRGRSVGASEDIP
                     GMRFIPFSSHTRMSGVDRGDVLIRKGAADVMFAHIRATGNGVSDQLEREVDAIAGAGG
                     TPLVVSRDGRALGVIHLKDIVKGGIRDRFTQMRKMGIRTVMITGDNRLTAATIAAEAG
                     VDDFLAEATPESKLRLIREYQEGGRMVAMTGDGTNDAPALAQADVAVAMNTGTQPARE
                     AANMVDLDSNPTKLIEIVEIGKQLLMTRGALTTFSIANDIAKYFAIIPAAFITTYPAL
                     AALNLMGLRYAVLSAVIFNALIIVLLIPIALRGVKYRPMSAEEALRNNILIYGIGGII
                     APFIGIKIIDILLSIMGA"
     gene            11924..13915
                     /locus_tag="VKO45_05305"
     CDS             11924..13915
                     /locus_tag="VKO45_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HMA05326.1"
                     /translation="MMARNSDLRPNPDLLLARVQEEERKGKRGRLKIFLGYAAGVGKT
                     YAMLKAAHLRRGEGIDVRIGYIETHGRPETELLLAGLAVIPTRSVEYRGVVLPELDLD
                     AVLAAFPRLVLVDELAHTNAPGSRHPKRYQDVEEILEAGIDVYTTLNVQHLESLNDVV
                     AQVTGVLVRETLPDRLIDEAAEIEVVDLPPPELVQRLMEGKVYVPELAASAIERFFKE
                     GNLYALRELTLRRAAERIDDQMLAYMQIHAIPGPWAAAESILVCIGPSPLSERLVRIA
                     RRQADRIHARWTALYVETPSHHRLSERSKEQAWRSLRLAEKLGAETATVFGLTVADTA
                     IDYAHRHNITRILIGKTLRPRWQEFLFGSVVDQLIHKSGTIDVYVISSSEGVSEPPVK
                     MEPLLPVTPPRSYLESIALVILMTAIGWLIRSVISPTNLIVFYLLAVVLSAYRWGIRP
                     AVLTAVLGVLAFDFFFVTPTFSFRVSDTEYLITFTGLITVGTVISLLVARVREHAFAA
                     QTRDQETSTLYALAQDLATAMDTDSILLAVSKHIEEIFQGKAVFLLPEEETLRVHAAS
                     LGLVLDADELAVATWAYRHRTMAGYGTDTLPGSRLRYIPIQSSRSALGVMGIKPPEPD
                     GAITPEQGRILLAFANQTALALERVNLAGRLGEASKREG"
     gene            complement(13953..14129)
                     /locus_tag="VKO45_05310"
     CDS             complement(13953..14129)
                     /locus_tag="VKO45_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05327.1"
                     /translation="MFLATEKVSEERTGRILAEIERAERIFRKMCDCGELDEEIRKAK
                     HAREETAAIRRILR"
     gene            14729..15817
                     /locus_tag="VKO45_05315"
     CDS             14729..15817
                     /locus_tag="VKO45_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HMA05328.1"
                     /translation="MLSEAFGYLEGIPLPALALDLRCRVIGLNKRFCGLLDLAGNTVM
                     GMDGFGLLDPGDREPVAALFRGLDREGSATLVTSLVGKNGRRTRVQAHWSRLSEATPG
                     GYLGLFEAMPVPSPAISARSPTPGGEETLPGRIDHSKRNQIIHTIIFHDAKNRLGALH
                     GYASLLRESLAGSGFLPYLDKLEEIGSDIERDLGVASILSHLGLIEPRWQNLREVIAK
                     LASREPEGRISLDGIPPSLFCLADPFFPRVFANLFENAGRHGERVTAIRIFTREDGAG
                     LVVSVEDDGIGVLADQKEKIFELGFGRHTGYGLYLAREILSIGGFSIRETGDPGKGAR
                     FEIRIPRGRYQLRPACPEEPDPVRIPAA"
     gene            complement(16302..17423)
                     /gene="dnaJ"
                     /locus_tag="VKO45_05320"
     CDS             complement(16302..17423)
                     /gene="dnaJ"
                     /locus_tag="VKO45_05320"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HMA05329.1"
                     /translation="MAKKDYYELLGIPRNASEDDIKKAFRHLARKYHPDLNPGNKGAE
                     EQFKEINEAFQVLSDPQKKAQYDQFGHAAFRPEDFAGFRTTNFEDLFRDSGFGDIFSI
                     FPGFGGGGRERARAPQPGADLRYDLNISFDEAFQGVTRTIEVPHSVECPTCHGTGAKP
                     GTLKDCPKCGGTGQVRSVRRSPFGQMVQISTCPQCGGSGKITTEPCETCQGRGKLRKV
                     SKIEVTIPKGIDDGQYLRVSGEGEAGERGGPPGDLYVVVHVAPHPIFERRGRDLYCKT
                     TIDFTTAILGGEVQVPTMTGTGGLKIPAGTQTHTVFRLKGQGMPGLNSDRRGDQFVKV
                     VVHIPEKITKKQRELLKEFSGEKKEETSAGFFEKLREFV"
     gene            complement(17446..19332)
                     /gene="dnaK"
                     /locus_tag="VKO45_05325"
     CDS             complement(17446..19332)
                     /gene="dnaK"
                     /locus_tag="VKO45_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HMA05330.1"
                     /translation="MPKEKIIGIDLGTSNSQAAVMLGGKPVIIPSAEGVTVAGKMFPS
                     VVAFTKEGELLVGEPARRQAVSNPDGTVMAAKRKMGTDHLYRISGKTYTPQQISAFLL
                     QKIRKDAEAFLGEEVKKAVITVPAYFNDNQRTATKDAGTIAGLEVVRLVNEPTAASMA
                     YGLDKGGEHKILVFDLGGGTLDVTIMEFGHGTFTVLSTSGDTQLGGTDMDQTVMDWIV
                     EEFRKQEGVDLKKDRMAVQRVREAAEKAKIELSTVVETEINLPYITADKEGPKHLTLK
                     LNRSKLEQLITPIINRCVHPLDTALADAKLTREGIDKVILVGGPTRMPIVQKFIEDRV
                     GKKVERGVDPMECVALGAAIQGGILAGEVTDLLLLDVTPLTLGIETLGGVRTTLIERN
                     TTIPTRKSQIFTTAADLQSEVTIHVLQGERPMAADNISLGRFNLVGIAPAPRGVPQIE
                     VTFDIDASGILNVTAKDLGTGKEQKMTITASTKLPDRDVDRMVKEAQQFAADDEKRKE
                     EATAKNEADSLIYTAEKTIADLGEKIPADLKEKIGSAVKATRSALEGKDIQKMKDETA
                     ALQKVLAEAGSAIYQEAQKKQAEEKARAGGSPSGEGSGESSGQGGPAGNEKVFDADFK
                     VKED"
     gene            complement(19304..19849)
                     /locus_tag="VKO45_05330"
     CDS             complement(19304..19849)
                     /locus_tag="VKO45_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HMA05331.1"
                     /translation="MTGEVSPQGEDPERKIARLEGELAEARRTAEERLTQLQYLQAEF
                     DNYRKSLEKEKVLAIQRAREALVAGLLPVLDDLDQALPCLEGEKNREGFRLLSRKLRR
                     FLEDTGLSPIESVGRPFDPRLHEAIAREESDRDDGIILEEFQKGYLLGSKVIRPSKVK
                     IAENTKESREENHAEGKDHRN"
     gene            complement(19939..21063)
                     /locus_tag="VKO45_05335"
     CDS             complement(19939..21063)
                     /locus_tag="VKO45_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMA05332.1"
                     /translation="MPGVLERRRDILRLMRSFTLDRGYFTVSDIARSTGLPRSTAQDW
                     INRLLAEGCLVVRKGRRGRSPARYLSKSAMPSSACRRIFTTIDGDAAEIYHECLSAGS
                     AAFCEFHHRRAGGILSRVSRDGTLLRERASLGSRDAPIGLFPDPSVGVTGLRRDGENL
                     VQSIRCVGGPAYSLTDMMAHAEGVEDVKVARSGAFVEGEVTTRALRFLAIGIDDTDSP
                     EGGATFALALALYQHLSTIGGVMPIGHHVVMLNPAHPGRTAGNSASFIELAAEPAVCP
                     EIRAHSLRFVSDEALSPEWGIAIKQGFTVPRDLRQFGNRVRTGEVTREEAEEIAREHK
                     IFTRGGRGIVGAVAAVSCIGLDNSILLDPKRAILPEEGGA"
     gene            complement(21047..22033)
                     /locus_tag="VKO45_05340"
     CDS             complement(21047..22033)
                     /locus_tag="VKO45_05340"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HMA05333.1"
                     /translation="MRVALLGFGSVGRGVARMIGEKDLPLTLTALADSKGGIIDPKGI
                     SIPEAFSRKENSGSCGRPKVSALDIVESDAYDVLVEVTPTNVDTGEPALTHIRGALNR
                     RRHVVTSNKGPIALAHRELTDLAATQGVALRYEATVCGAIPIIHTLQHGLLGNTVAAL
                     FGVMNGTCNYILTRMEDEGLTYDQALGEAQEMGYAEKDPTYDVKGLDAAIKLVILANT
                     IWRMDAGMKDVEVTGIDRITPEAIQLAKDGETAIRLIGEAVPSRNLLRVSPRILPRNH
                     PLLVEGTLNAVTVVTDMAGELTFIGKGAGSIETASAVIGDLLSIREMYAGRP"
     gene            complement(22030..22521)
                     /locus_tag="VKO45_05345"
     CDS             complement(22030..22521)
                     /locus_tag="VKO45_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HMA05334.1"
                     /translation="MKLEMKDVPGQLVRVLEPISACGGNIIAVIHQRDPESQSGTIDV
                     QIVLDLPSGRLPALVDQIKSLGVDIIRLGEERLLHQRALIMIGHIMHTDISDTVDQID
                     RTGYAEVSELSLMMPGISERSSARITIRAANRGDMESAVGILREVAARKEILVVEPLE
                     DGP"
     gene            22670..23107
                     /locus_tag="VKO45_05350"
     CDS             22670..23107
                     /locus_tag="VKO45_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HMA05335.1"
                     /translation="MKVRVIACGNPLMGNDAIGPSVMALLAKEHPEVDVVDGGTGGLG
                     LIPLMEGYDRIVIVDATSGMGKIGDVRVIRDIPAGGIASLSLHELGIPEALGLARTLG
                     ISPGVVIVGIEGGTIPGFSGEMDPAVRAAIPEACRRVLEELRR"
     gene            complement(23120..23830)
                     /locus_tag="VKO45_05355"
     CDS             complement(23120..23830)
                     /locus_tag="VKO45_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HMA05336.1"
                     /translation="MTETKPKISYAGIAETLKGALSLAGSPVAVKLAKSREEVPAGVP
                     EGKETVRHCQMVNMARKEGAIFYAPSAKHQCNGGAWALGLREITPTLQSGEFYFKLGK
                     FESMAACRRTMDKIPHLRTGETYATIYAPLEKTPFDPDVVLVVTTPRNMLKLAQASIF
                     RLGGRLSPAMAGIQSVCSDATAQPYLTGAPNFSLGCDGSRKFSGIADDEMVMGIPAEL
                     LPEIGEALRIVTTAPGSK"
     gene            complement(24058..24870)
                     /locus_tag="VKO45_05360"
     CDS             complement(24058..24870)
                     /locus_tag="VKO45_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HMA05337.1"
                     /translation="MRILVVSRVDDHRAVDFARQLAGDLRDWHHEVILEDALARRLGV
                     PGTDLGEAGADLAVVIGGDGSVLYTVQGLKSQIPILGINWGEVGFLAELEPGEARGFL
                     EGLREGFRVERRMRIGITLDGAGIGEALNEVVIVTSRPAKMLRFTVEVDGILAERFRA
                     DGMLVSTPTGSTAYAMSAGGPIVDPRVEGLLLVPLAPYMLSSRPVVIAGDRVLRITLE
                     SEKPARLVIDGQQTRELALGAVLTIRRSESPALFVDVGKNFFDKVDRKLRRL"
     gene            complement(24872..25681)
                     /locus_tag="VKO45_05365"
     CDS             complement(24872..25681)
                     /locus_tag="VKO45_05365"
                     /EC_number="3.1.3.11"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     fructose-bisphosphatase/inositol-phosphate phosphatase"
                     /protein_id="HMA05338.1"
                     /translation="MAYHKYHAEIMAFLARCEEMARAVEDIVQDLVARPEGGRVVRTG
                     ASGTPTRQIDQAAEDWIVEYIGTHALCRTLVSEECGKRENMGGERGTIFLDPVDGTHN
                     ALAGIPFYALSIAYAEEGTVRQGFVRDLAHHETFTAERGKGAFRDGRPIRVSRTMLLE
                     ESAMSVYGRKFNPAPVLFLGQKIRRWRLLGASALELCYVGSGRLDGFIDLRATLRVTD
                     AAAGMLVCEEAGGKVSDLDGRGISFPDEVTVGKCLVASNGTLHGKVIEYLR"
     gene            25717..26094
                     /locus_tag="VKO45_05370"
     CDS             25717..26094
                     /locus_tag="VKO45_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HMA05339.1"
                     /translation="MKEQTEVGKLKEGRYVVIDEEPSKILGIATSKPGKHGAAKSRID
                     AVGLFDGVKRSIVQPVSAKIYVPMVERKGAQVISKAGRVIQLMDVKDYTMFELTVSEE
                     EAARIETGKDITYIESMGKRKLD"
     gene            complement(26121..26780)
                     /locus_tag="VKO45_05375"
     CDS             complement(26121..26780)
                     /locus_tag="VKO45_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05340.1"
                     /translation="MEKQDILVVVIAIVAFAVLALLVRPLLVGPIAGTGPGTGIRTTP
                     TVAVTPSVTQVVTPAKTVPTLPPTTPAPTPTWDGSVKSVGFVGQPEGQVTPTPNPTIP
                     QPSLQNRSLVTYATISNRWPGTTENVYVPAPWWVLEYTAEPMALPPNAYPMLIIQVFD
                     AQDPNRLVIAPIRQYIYEEPSDTPWSQKLYEGRRTYYFRVDTSFIKTYTIRIKVPLEY
                     T"
BASE COUNT         4491 a         8846 c         8423 g         5197 t
ORIGIN      
        1 taccacgatc ctcttccgga ggtcgcgggc caggatcttg atgaggtcgg cctggtccgc
       61 gaacttcttc gggtcctcgg tgtagatgac gagctcgggg ccctcgaact cgacctggga
      121 gaccgagatc ccggcgggta cgataccgtc gatcttttcc ttgagatccc ttaacctgtc
      181 ctcaatggtc ataaaaaaac cgtccgtcgg aaaaaagggg ttatgcagag gcagaaatgg
      241 ggtgtttctg gccgttctcc acgatgaact cctcgtactt ctccttcgtg atgacgacga
      301 cgttgatgtc ctcgcccgag gcgatgtccc ggcgcatggc ggcccggagg gcccgcaccg
      361 cgaggtccac cgcctcctgc tcccccatgt caggccggta gcggtcctcg agcacgccgt
      421 aggccatggg cgaaccggag cccgtggaca cgatctcctc ctcgcgggtc gcgcccccca
      481 tcgcatccac cgagtagacg ctcggccccg tttcgtcgac gcctcccacg aggagctgga
      541 catagtacgg gtagtaccgg ttctggttca ggtagttcga gaggagcgtc gaggacgccc
      601 cgaccgagat gggccttccc cgccggatct ggtagaggct gcactcgacg gtaatgatcc
      661 gggcgagctg ctgggcatcg cccacccccc ctgccgtggt catcccgatc cggtccgcga
      721 tctggtagac cttcttcgcc ttcttgctgg ccacgaggtt ccccatggtg gccctcctct
      781 cggtggcgag gatcacgcca tccttgaaga cgagcccgat ggtggtcgtc cccttcatgg
      841 tctcctggga taattcctgc aaagagttta cctccttttg aaaaacgcca acgtctataa
      901 gagtgtcaat ccatacaacg cgcttctttc acttaaatat tatgattgtc ccccatcgca
      961 gaggacccgg atgatctccc ggtcgaagag catccccacg aggcgcttgt cgctgttcac
     1021 cacggggagc tggtccaccc gggcccgctt catcttgagg gcacactcgc cgacctcgga
     1081 gttcaggggc accgagacga cgttcgtgac catcgcggtc ttcaccggcc gggccgggag
     1141 cttcaccttc gagacgccga aggagagggt gtggaggtcc cggatgctct cccaggtcca
     1201 ctcgtcgtcg tccgtgccat tggagaagtc cgaggtctcc accgagtcct cgatggagga
     1261 gtgcttgatg aggtcccgct cggagatgat cccggagaga accccctcgc cgtccaggat
     1321 ggggatggcg tccacccccg agatctccat gacccttccc acgaggacga ggggggtctc
     1381 ctcccagagg gcgaaggtgg tgctcgtgag ccggtccttg atctccatcc ggttcttctg
     1441 ctgggcgatg gcggtcacga ggtccgcgac cgagagaatc ccgacgaggg ctttgtcctc
     1501 gaccaccggg agccggcgca ccttcttctc gatgatgagc ctggctgcct cctcgaggga
     1561 ggcatcgggg gcgatggtcg ccggattccg ggtcatcagg aggccgagct gggtctcctc
     1621 cgacttccgg agcatgtcct tcctcgtgat gatcccgacg agcttcccct ctttgagaac
     1681 cgggacgccg ctgatccccg tgcgcttcag gatcttgagg acgtcgtcgc ggttccccgg
     1741 aatctcgacg gacaccacgt caggggtcat gaaatccctg acctctgtcc ccgtcatgac
     1801 ctcaccacca gggtggagac cggggagtgg ctcacgacga aggaggagat gctccccagg
     1861 aggatccggt ccaggttgct cttgccatgg gagccgagca cgatcaggtc tgcacccgtt
     1921 tcccgggcga gcttgatgat ctcgtttccg gcatggccct ctttcaggtg ggtggtgatg
     1981 gcgaccccct gggcctgggc ccgcttcgcc gcggcgaggt gggcctcctt cccctggttc
     2041 tccaggagcc cgtagatcac ctcccaggtg ttgtccatgg ggatggacga gaagagcccc
     2101 gtctcgatga cgtagacgac atggagcgcg gccccgtgaa ctttcgcttc cccgatggcg
     2161 atgtccaggg ctgcctcgct cttcttcgac ccatctatcg ctgcaaggat ggattggaac
     2221 ataggcacac tcaaaatggc tgcgttacgt acttatccgg tgaaaataaa tatttttccc
     2281 tgatatccga gggccccgtc ctccgtacga gggaggcgac cgggtcccgg ctgaaggaga
     2341 ggttggatcc cgccgggttg atgaccagga atcgcgccgg ggagccctcc tcgatgaaac
     2401 ccccggaaag aaaccgcccc gatccccgga tcgccgccgc gaggatctcc cggggatgga
     2461 tcggatagac ggtgctcgca aaggccatct ccgcccagag gtcgggctgg acgaacatgg
     2521 cattgtcggt cccgaggagc agggtgaccc cgaatgtgag catccgctca aggggcgggt
     2581 gatccctcga tgacgagacc ccgaggatcc agttggaccg ggggcagatg gccacgggga
     2641 tatcccggtc ggcaatctcg cggaggtgcc ggtcctcggc atgggtgcag tggatcagga
     2701 gatccggttc gagcgcgagg gcaccgtcga tgtcgagggg atcccgttcg ccggcatgga
     2761 aggccacgag tttcccttcc cttcttgcgg cggaaacggc cgcctcggca tccgaaaagt
     2821 cccggacact cgggatcccg agtccctcgg cgatcccctg ccccccgtcc cggccaaaga
     2881 tgaccggccg gcaggggagg ccctccgccg ccgcccggag gtcacggacc cccgcctccc
     2941 cgccctcccg gaagtcggcg aagcccgccg tccctgacct gaccatcacc ccgatgctcg
     3001 cgcgcatccc ctcccggagg gtgcgcctgg ggcttgtctt gagaaggcgg tgcttcaggc
     3061 cgccgggcgg ggtgaccagg tcctcgaggg ggcctgacca tggcatgtcc atcccgacgg
     3121 tatccccgag atgcgtgtgg gcattgaaga gggccgggca gatccagacg ggaggagggg
     3181 aggagatctc ggcgatctcc cggatcaccc cctcctccac ggtaatccgg acgggccgga
     3241 gatcgagatc cctgccgacg atggcatgcc cgctcacctg gaagtctgcc tgcgtcatcc
     3301 ctgataccta tttatacctt gtccgggtaa tagctgtgca tggcaaaaaa gcaggggggc
     3361 aggctcatct cctcggcggg cctcgtcaac tactacgaga gcacggaccg gcgtgccctc
     3421 cagatcagcc cctatacggt ggccgtcgtg gcggccgcca tcggggtggc aattttcatc
     3481 ctgaacatcc tctactaggc atttcccttt tttccgtcgg tatcaggatt ccggggtcct
     3541 tcccttccca ccagtcgacg ttcccgtcta cgagaaacta tatcatccga cacagggatc
     3601 tcccattgtg tatatcgtga tccgctgccc gggctgcagc accttcacct acgtggaccg
     3661 cttccagcgg cacaagctct gccatatctg cggggaggtg atcgatgtca ggaccgcccc
     3721 cgcgtacctc gaggtgaagg attaccccga tgccgaggag gtcgtgaacc agctccagag
     3781 cttcctcgag agggagggca ggaaggatct ctcgccgcag gagctccgca tgctccgggc
     3841 aaaatacgcc cagtggctcc tctcccagac ctgatcacca cggccggccg cagccggggc
     3901 agacggtggt gattctcctc ccgcacttct tgcagtagag gccgatcctc tcgtcgtggg
     3961 agagggggag gtggcagttc ttgcagtgga aattggcctc gtacccgcag ctgtgccgaa
     4021 gcattgatca agggtatccc ggcccgtgct tataaaggaa tcccaggtac cggttctccg
     4081 atttcggagc cgtatgcgac ggaagggacg ggctacaggc ccccggttcc ccggaattcg
     4141 cgggattctt tatacgtaga ggccccatcc ttatccggcg atccatgacg caccacctcc
     4201 ggcggaagat cctccttgcc atcctgcttc tcgccattgc ggccatcttc gccctgttct
     4261 ggcactggct ggacatgatc cccttcggga tcgccgtggc cgtcgtggcc gccccggcga
     4321 agaaccggct ggaacgccgc ctgcccgggt ggctctcggc cctcgccatc gccctcgtga
     4381 ccctcctcct cctctctgcc gggatcctcg tcaccatcca ggcgatgcag gagaacctcg
     4441 ccacgaacga ggagatcctc gggcggatcg cgtcgggcat cgaggccctc tcgccccagc
     4501 tcaccaccgc agggattccc gaggggacgg tccatacggc cgtcgcctgg atcgagggct
     4561 ctgtccaggc cctcgcgggc ttctggctct ccctttccct cgagtccgtg ctcctcaccc
     4621 cgagggtcgg cctcttcttc ctctccctcg gactcgccct ctggaagggg gaggcggtcc
     4681 tccgggcggt ccttccccgt atcccgccgt cgtggctctc ggtgtacccg aacctggccg
     4741 cggtctcggt ggacaccctc tacgcggtca tcgtggtcca cctcctcatc gtgggcctta
     4801 ccttcgggct ctcccttccc ttcttctgga tcctggggta tgggcacgtc ctctacctct
     4861 ccctcgtcac cgccttctgc gagctcgtgc ccgtcctcgg ggcctccatc cccatggtgg
     4921 tcctcctcct ctattccctg gccctcgggg acctccgggg gttcctcctc gtcttcttca
     4981 tcgggtacct ggtggtggcc ctcctcccgg aggtcacgat caggcccatc ctcatgggga
     5041 ggcgtacgct cctctccccg gtcctcatgt tcgcgagctt catcggcggc atcctcctcc
     5101 tcggcatctc ggggttcctc ctcggccccc ttgtcgcggc cctcggggtg tcgtggtaca
     5161 ggctgcggat ggagggaagg acggggcaga ctcagcctcc ctgagcaacc ggttttctcc
     5221 cctggggaag ggatttatcg cctgcgagcc tgaacccttg tactgcgagg gttgccaagc
     5281 ccggccaaag gcggcggatt tagggtccgt tcccgaaggg gttcaccggt tcgaatccgg
     5341 tccctcgcat cagatgtcct cgtcaggaaa tcctggccgg ctcaagatgg cgtcaccggc
     5401 tccaaccctc caccagactg ccctccacct cgaatacttc acggtcggct acaacattgt
     5461 cgaggcggtg gcatccatcg ttttcggcaa cctggccggg tcgattgccc tcatcggctt
     5521 cgggctggat agcatcgtag aatccctctc gggactggtt ctcatctggc gtctccgccg
     5581 gcacggtcat atctcaagag acgaagaaga acgtgtcgaa cggagggcac agcggttcgt
     5641 cgccctgacc ttcttcatcc tcggcgccta cgtcctctat gagtccgggg cgaagctcat
     5701 cttccgcgag gccgcccagc cttcctccgc gggaatcatt atcgccctcc tctccatcct
     5761 catcatgccc atgctcgcct ggaggaaaca gcaagtcggg atgggtatcg gcagcagggc
     5821 gctcatagcg gactcgaagg agacccttgc ctgtgccttc ctctctgtcg gcctcctcgc
     5881 gggcctcggg gcaaatcagc tcttcgggtt ctggcaggca gaccccctcg ccggtctctt
     5941 catctcactc tttctcttca gggaaggata cgaaggatgg cgggaatccg gggatgagaa
     6001 ggctgatcag gattgacctg ccgacgaaag ggcaccccga cttcttcccc tgcagacacc
     6061 tgaacatctt ttccttcacc cacgcgggat ccttggcatt ttcctggtga accggatagg
     6121 gggaatggta gagggccgat ctcctcccga tcctctcgcg attctcggaa agattatttg
     6181 ggtggcttgt acccccctcc catctccttc tccacctgcc ccgccagttt cgccttctcc
     6241 ttctggttct cgaggttctg ggcgagggcg accacgccgg tcgtatcacc caggttcatg
     6301 gtggaaagga ccgacgcggg aacgatgacg agggtcgggt tgcccgtctt caggccctcg
     6361 aagagcatgt tcatggctct caggtgcagt gccgtcgggt tgtcctggta ggtccttgcc
     6421 gcctcctcga acttctgggc aatctgcttc tcggagtccc cgaggatgac ccgggcctgg
     6481 cgctcccgct cggcctgggc ctgcatggac atcgcgttct gaagctcctg ggggatgagg
     6541 atgtcccgga tctccacgga gctgatgttc acgccccact gctcggtccg caggtcgatg
     6601 atgtgctgga gttccttgtc gagcttctcc cggttctcga gcatgtcggc gagcatcgac
     6661 ttcccgatga tctcccggag ggcggtctgg gaggcccagc tgatagcggt ccggtagttc
     6721 tccaccgcga gggcggcctt ctccgagtcc accaccttcc agaagagcac cgcctcaacg
     6781 tcgacgggga ccgtgtcctt cgtgagcgtt ttctcggcca tgaaggtggt cgtgatgatc
     6841 cggaggtcga tccagtacgc cacccggtcg acgatgggaa tgatgaggaa ggggccggcg
     6901 cccttgaggc cgaaatactt cccgagccgg aggaccaccg ccttctccca ctggtcggcg
     6961 accttgaagg aggcggcaat cacgatggcg atgaggccga tccagaggat cgcccagaag
     7021 gtcgtcatga tattcatgcc ggagagggtc caggcaagcc atgctcccgg gatcacgatg
     7081 aggaagaaga tcagggtctg gattgcatat gacttttcca tggtctctcc ctatcggggt
     7141 ctacctatat aagactggct caggcggtat gggtcccttc cccccttgat ggtgcgcggg
     7201 ggatactttt aaggataatt tcatggaatg cctcttcccc ttcccggctc tttcccgacg
     7261 tgacgatacc ccatcaaatg aaattgcccg ggtgacgatg ggcacggata tcaagtacca
     7321 taaatagtta caacctgcac gctggatcag gggtacattc ctccaggtgg gaggggcccg
     7381 ggatgggtga tccggccgtg ttgcgggaaa ccgtttccat ggcaaggagc cagctcaggc
     7441 ccgccctcgt catcttcctg ctcctcaccc tcctcaccgg ggtcctctac ccggtcctcg
     7501 tcaccggcat cgcccagctg gtcttccccg tccaggcgaa cggggatctc atggtgcacg
     7561 acgggaaggt cgtcggatcc tcgctcatcg ggcagccctt ctcctccccg gtctacttct
     7621 ggggacgccc ctcggccacc gccctcgtcc cttataatgc gagtgcctcc gggggctcga
     7681 atctggggcc ctccaacccg gtgctcctcg atgcggtaca ggcccgggtc gatgccctcc
     7741 acgccgccga cccggcgaat acccggctca ttccggtcga cctcgtcacc gcatcgggga
     7801 gcggcctcga tcccgatatc agtgtcgctg ccgcgtacta ccaggtcccc agggtggccc
     7861 gggtgcggaa cctgagcgag ggggaggtgc aggggctggt cgccggtcat atccagggca
     7921 gacagctcgg gatcttcggg gagccgcggg tgaacgtcct ttcgctgaac ctcgcactcg
     7981 atgacctggc gacccggggc ccgggctcgc cggcccctga tctcgtcccg gtctctcagg
     8041 ggagctacaa cgagacgccc gtcccgggta tgcgggggtc cgactggctc cagctcatat
     8101 ttttcttcgc cgcgggcgcg gccctcatta tccccctcgg ggggttcatg gcaaaggtgc
     8161 ttacggggga accgacgttc ctctccccgc tcaccggccc aatcgagggc tggctcctcc
     8221 gctcatcggg agtgaagccc gggcaggaga tggactggag gatgtttggc attgccctga
     8281 tggtcttctc ggggatctgc ctcatcgtgc ccttcctcct ccaggagacc cagcagctcc
     8341 tccccctcaa cccctccggc cttggcccgg tgccgtggga cctctccctg aacaccgcgg
     8401 tcagtttcgc cacgaacacg aactggcagt tctatgtccc ggaaaccacg gtgagctacc
     8461 tgacccagat ggccgggctc gccgtgcaga acttcctttc cgcggccaag gggataacgg
     8521 tcctcgtcgc cctcgtcctc gccttctcgc ggagatcggc acagaccgtg ggtaacttct
     8581 gggtgctcct cgtccgggcg gtcatgatcc tcctgccgct cagcctggtc ctcgcccttc
     8641 ttctcgtttc ccaggggagc gtccagacct tcggcgggcc cgtgaccgtc cctctcctcg
     8701 acccggtccg ggacgcctcc gggaacctcg tgaccacgca gctcatctcc gtcggtcccg
     8761 cggcatcgca gatcgccatc aagatgctcg ggacgaacgg cggagggttc ttcaacgcca
     8821 actcggcgca tcccttcgag aaccccacgc ccctctcgaa ctttatcgag aacctctccc
     8881 tcatgatcat cgccgcggcc ctctgttaca gcttcgggca gatgatcggg tcgaggcgga
     8941 aggggatcgc cctcctcatg gcgatgacca tcctcttcct ccccctcgcg gggatcgcca
     9001 tctggtccga gctgggaggc aatccggcct ttgatcccat gggcgttgac cagagcccct
     9061 tccattccca gcccgggggg aatatggagg gaaaggaggt gcggttcggg atcgtcccct
     9121 cggccctctg gtccaccgca acgacctcga cctcctgcgg ggccgtgaac tccatgcatg
     9181 actcgtacat gcccctcgga ggcttcgtcc agatgttcgc catgcagctc ggcgaggtgg
     9241 tgtacggcgg cgtcggctcc gggctgtacg ggatgctcgt cttcgtggtc atcgccatgt
     9301 ttatcgccgg gctcatggtc gggcggaccc cggagctgta cgggaagaag atcgagccgc
     9361 aggagatgaa gctcgcgacg gtcatcatcc tcatccccat cttcgtgacg ctcaccggga
     9421 cggccattgc cgtgctcacc ggcccgggga cggcaaccac cctcaacccg gggccccacg
     9481 gattctccga gatcctctat gccttcacct cgacccccca gaacaacggg agcgccttcg
     9541 ccggtctctc ggcgaatctc ttctacacca tctccaccgc catcgccatg atcatcgggc
     9601 ggtatgcggt cgccctcttc acccttgccc tcgcgggttc ctttgcggga aagaagatcg
     9661 tcccggcgag cgaggggacc ctccaggacc accgcccgct cttcatcatc tggctcgtct
     9721 tcgtggtggt catcgtcggg gccctcagct acctcccggc gctctcgctc gggcccatcg
     9781 ctgaatacct gatccagatc gggaggggtg cgatccatgc ctgatcccat ccgggcgagg
     9841 gggcccgatc cggccccgcc tgccctctac cggcgggcca tcctcgacgc cgtgatcaag
     9901 ctcgacccga ggacgctctc ccggaacttc gtcatgctcg tggtggagat ggggagtctc
     9961 ctcaccaccc tcctctgggt gcaggccctc ctcggccacg gggaggcacc cgcggggttc
    10021 atcggggcca tcgcggcatg gctctggttc accgtcctct tcgccaactt cgccgaggcc
    10081 ctcgccgagg gaagggggaa ggcccaggcc gaggcgctga ggaggatgcg ccaggacacg
    10141 ctcgcaaaga ggctggaccc gtgcctccgg cgcgagagga agaaggagcc gggaccatcg
    10201 gatttcaccg tcgtatcctc gtccgagctc cgcagggagg acatcttcta tgtgaaggca
    10261 ggggacgcca tcccggtcga tggggaggtc atcgagggca ttgcctcggt gaacgagagc
    10321 gccatcaccg gggagagtgc cccggtcatc cgggagtccg gcggggaacg gagcgcggtc
    10381 accggaggga ccaccctcct ctccgactgg ctcgttgtcc gtgtcagtgc aaaccccggc
    10441 gaggggttcc tggaccacat gatcggcctc gtggaggggg cgaagaggca gaggaccccg
    10501 aacgaggtcg ccctcaacat cctcctcgtc gggctcaccg cggttttcat cgtggtctgc
    10561 gcctctctcc gggccttttc ggtgtacagc gtccgggcgg ccggcggcgg cgtgcctgtc
    10621 actctcaccg tcctcgtcgc cctcctcgtc tgcctcatgc ccacaaccat cggcgggctc
    10681 ctctcggcca tcgggatcgc gggcatggac cggctcatcc ggaggaacgt catcaccacc
    10741 tcgggacggg ccatcgaggc ggcgggggac gtggacgccc tcctcctcga taagacgggc
    10801 accatcaccc tcggaaaccg gcaggccgtt gccctgatcc cggtcgacgg gaccgatatc
    10861 acggagctcg ccgaggtctc ccagctcgcc tccctcgccg acgagacccc cgagggccgg
    10921 agcatcgtcg tcctcgcgaa ggagcggtac gggctccggg gaaggtcggt gggcgcctcg
    10981 gaggacatcc cggggatgcg gttcatcccc ttctccagcc acacgaggat gagcggggtc
    11041 gatcgcgggg acgtcctgat ccggaagggg gcagccgacg tgatgttcgc ccacatccgg
    11101 gccacgggga acggtgtctc cgatcagctg gagagggagg tggacgccat cgcgggcgcc
    11161 ggcgggaccc ctctcgtggt ctcgagggac gggagggccc tcggggtcat ccacctgaag
    11221 gacatcgtga agggcgggat ccgggaccgg ttcacccaga tgaggaagat ggggatccgg
    11281 accgtcatga tcaccgggga caaccggctc acggccgcga ccatcgcggc cgaggcgggg
    11341 gtggacgact tcctcgccga ggcgacgccg gagagtaaac tcagacttat ccgtgagtac
    11401 caggaggggg gaaggatggt cgcgatgacc ggtgacggga cgaacgacgc ccccgccctc
    11461 gcgcaggccg acgtggccgt cgcgatgaac accggcaccc agcccgcgcg ggaggcggcg
    11521 aacatggtgg atctcgacag taacccgacg aagctcatcg agatcgtgga gatcgggaag
    11581 cagctcctca tgacccgggg cgccctcacc accttctcca tcgcgaacga catcgcgaag
    11641 tacttcgcca tcataccggc cgccttcatc actacgtacc ccgccctcgc cgccctcaac
    11701 ctgatgggcc tccggtatgc cgttctctcg gcggtgatct tcaacgccct cattatcgtc
    11761 ctcctcatcc ccatcgccct ccggggagtg aagtaccggc ccatgtcagc ggaggaggcc
    11821 ctccggaaca acatcctcat ctacgggatc ggcgggatta tcgccccgtt catcggaatc
    11881 aagatcattg atatcctcct ctcgatcatg ggagcgtgac aggatgatgg cgagaaactc
    11941 cgatctccgg cccaacccgg acctcctcct cgcgagggtg caggaggagg agcgaaaggg
    12001 aaagcggggc aggctgaaga tcttcctcgg ctatgccgcg ggggtgggga agacctacgc
    12061 gatgctcaag gctgcacacc tccggagggg ggaggggatc gacgtgagga tcgggtacat
    12121 cgagacccat ggcaggcccg agaccgagct gctcctcgcg gggctcgccg tcatcccgac
    12181 gaggtccgtc gagtaccggg gggtagtact cccggagctg gacctcgatg ccgtgctcgc
    12241 cgcattcccc cggctcgtcc ttgtcgacga actggcccat acgaatgcac cgggctcgcg
    12301 ccacccgaag aggtaccagg acgtggagga gatcctggag gccggcatcg acgtgtacac
    12361 gaccctgaac gtccagcacc tggagagcct gaacgacgtc gtggcccagg tgacgggggt
    12421 cctcgtccgg gagacgctcc cggaccggct catagacgag gcagccgaga tcgaggtggt
    12481 cgacctcccg ccccccgagc tcgtccagcg gctcatggag gggaaggtct acgtccccga
    12541 gctcgccgcg agcgccatcg agcggttctt caaggagggg aacctctacg ccctgcggga
    12601 actcaccctc cggcgtgcgg cggagcggat cgacgaccag atgctcgcct acatgcagat
    12661 ccatgccatc ccggggccct gggcggccgc cgagagcatc ctggtctgca tcggcccgag
    12721 tcccctctcc gagaggcttg tccggatcgc ccggcggcag gcggaccgca tccatgcccg
    12781 gtggacggcc ctctacgtcg agacgccctc ccaccaccgg ctctcggaga ggtcgaagga
    12841 gcaggcctgg cgttccctcc ggctcgccga gaagctcggg gcagagacgg caacggtctt
    12901 cggcctcacc gtggcagaca cggccatcga ttacgcccac cggcacaaca tcacccggat
    12961 cctcatcggg aagaccctgc ggccccggtg gcaggagttc ctcttcggct cggtggtgga
    13021 ccagctcatc cacaagagcg ggaccatcga cgtctatgtc atcagcagca gcgagggggt
    13081 ttccgagccg ccggtaaaga tggagcccct tctcccggtc acaccccccc ggagctacct
    13141 ggaaagcatc gccctcgtga tcctcatgac cgccatcggg tggcttatcc ggtcggtcat
    13201 ctccccgacg aacctgatcg tgttctacct gctcgcggtg gtgctctccg cgtaccggtg
    13261 gggcatccgc cctgcagtcc tgaccgcggt gctcggcgtc cttgccttcg actttttctt
    13321 cgtcaccccc accttcagct tccgggtgag tgataccgag tacctcatca ccttcacggg
    13381 gctcatcacc gtgggcaccg tcatctccct cctcgtcgcc cgggtgcgcg aacacgcctt
    13441 tgcggcccag actcgggacc aggaaacgag caccctctat gccctcgcgc aggatctcgc
    13501 caccgccatg gacaccgatt ccatcctact cgccgtctca aagcacatcg aggagatctt
    13561 ccaggggaag gccgtgttcc tcctgccgga ggaggagacc ctgagggtgc atgcggcaag
    13621 cctcggcctg gtcctcgatg cagacgagct ggcggtcgcc acctgggcgt acaggcaccg
    13681 gaccatggct ggatacggaa ccgatacgct ccccggatcg aggcttcgct acatccccat
    13741 ccagagttcc cggagtgccc tcggggtcat ggggatcaag ccgcccgagc ctgacggcgc
    13801 cattacgccg gaacaggggc ggatcctcct tgcctttgca aaccagacgg ccctcgccct
    13861 ggagcgggtg aacctggcgg gaaggttggg ggaggcctcg aaaagagagg ggtaagggag
    13921 gggagcctga tctacctggt cagggaggcg tatcagcgga gtatacgcct gatggcagcg
    13981 gtctcctccc gggcgtgctt tgccttccgg atctcctcat cgagctcccc gcaatcgcac
    14041 atcttcctga agattctctc cgcacgctcg atctctgcga ggatccttcc cgttctctcc
    14101 tcggacacct tctctgtggc aaggaacaat ccagatccct caaatcctga ataggtgatg
    14161 tgatgcgaat aagtatttaa tgttaatttc gcccataaat tttataaaat taattgactt
    14221 ttattgacct cccgggaggc caagcttcat gggtgaccgg aaaaatccat ggatttcgca
    14281 tatctaactg aaaatcatct ggatacgtca tattgcctgg agaaaatcat tatatagaat
    14341 aacatacttt tgatgaattt ccttgatttt gctgttttgg tggagaggac ggccgagtca
    14401 tctccgggca ttacatcgtt ttgttgtcca tccttcccaa ctcgccggtt ctctcccaca
    14461 ttctctctcc ggcctcaggc accgcgcgca aaaatccggc tgggctgaga gctgcctggg
    14521 accaggtacg gaccagatca aacggtcgga cgacggtctt gcagaggccc cttcgttcat
    14581 caagagaggc accggcagcg ggagctcccc tgtaaaactt catatattcg tctggatgcc
    14641 aattaaaagg cccggagaaa aatcaaatga tttaagtata tgtctgcgat tggtaccgtt
    14701 attgggagtg tggtggcggg tgaagggggt gctgtccgag gcattcggat atctcgaggg
    14761 gatacccctt cccgcactgg ccctggatct ccggtgcagg gtcatcggcc tcaataaacg
    14821 gttttgcggc ctcctggatc tggcagggaa taccgtcatg gggatggacg gtttcggcct
    14881 tctggatcct ggagaccggg agccggttgc cgccctcttc agggggctgg atagggaggg
    14941 cagcgccact ctggtcacct ccctcgtcgg aaagaatggg cggcgcacga gggtccaggc
    15001 acactggtcc cgcctttcgg aggcaacccc gggcggttac ctcggactct ttgaggcgat
    15061 gcccgttccc tcgccggcga tatcagccag gagccccacc cccgggggcg aggagacgct
    15121 cccgggcagg atcgatcact cgaagaggaa ccagattatc cacaccatca tcttccacga
    15181 tgccaagaac cggctggggg ccctccacgg gtacgcctcc cttctcaggg agagcctcgc
    15241 gggctcgggg ttcctcccgt atctcgacaa actggaggag atcggttcgg acattgagcg
    15301 ggacctgggg gtcgcctcca tcctctccca cctcgggctc atcgagcccc gctggcagaa
    15361 cctcagggag gtcatcgcga aactggcctc acgggagccg gagggaagga tctccctgga
    15421 cgggatcccc ccctccctct tctgcctcgc cgaccccttc ttcccccggg tcttcgcgaa
    15481 cctcttcgag aatgcgggga ggcacgggga gagggtgacc gccatccgga tcttcacccg
    15541 ggaagacggg gccgggctcg tcgtctcggt cgaggacgac ggcatcgggg ttctcgcgga
    15601 tcagaaggag aaaatcttcg agctcgggtt tggccgtcac accgggtacg gcctctacct
    15661 cgcccgggag atcctctcca tcggggggtt ctccatccgt gagaccgggg atcccggaaa
    15721 gggggcgagg ttcgagatcc gtatcccgag aggcaggtac cagctccgcc cggcctgccc
    15781 ggaggaaccg gacccggttc ggatcccggc ggcgtgagga tcgatctccc ggttgttttc
    15841 tccctttcgt taaattcgag attcaccaac tatctaccga tccgaactga atccttgact
    15901 ggtggacgct cccgggggaa tggaggtttc gtagaaaccg acatggagca acccgccata
    15961 aggcggggtg cgactcgcct cccggctcgc ccccgccgct gcggcgtccc ccggattacg
    16021 ataggccgtt tattccatgt accgtttcat cagaagatgc cggaaacagg ggggtgcccg
    16081 tgcggcgggg gtggagggag gtttcgtaag agggagggtt catcagaacc cgacctcgag
    16141 cgacgaagtc gcgaagaaac cgacctggag caacccgcca tcaggcgggg tgagactcgt
    16201 agcccccctt agagcaccac actaaaatga atcacgatgt cattgggcct tttcagattc
    16261 aaaataaatt cgagataagg tcgaatcacg ccgatcattc gtcagacgaa ttcgcggagt
    16321 ttctcgaaga agccggcgct cgtctcttcc ttcttctccc cggagaattc cttcaggagc
    16381 tccctctgct tcttcgtgat cttctcgggg atatggacca ccaccttgac gaactggtcc
    16441 ccccgcctgt ctgagttcag gccgggcatt ccctgaccct tgagccggaa gaccgtgtgg
    16501 gtctgggtgc cggccgggat cttcaggccc cccgttcctg tcatggtggg gacctggacc
    16561 tcccctccca agatagcggt agtaaaatcg atggtggtct tgcagtaaag gtcgcgcccg
    16621 cgcctctcga agatgggatg gggcgcaaca tgcacaacca cgtagagatc cccggggggc
    16681 ccacccctct ctcctgcctc gccctctcca ctgacacgga ggtactgccc gtcatcgatc
    16741 cccttcggga tggtcacctc tatcttcgac accttccgca gcttccccct tccctggcag
    16801 gtctcgcagg gctcggtggt gatcttccct gatcccccgc actgggggca ggtggagatc
    16861 tggaccatct gcccgaaggg ggatctgcgc acgctgcgaa cctgtcccgt gccgccacac
    16921 ttcgggcagt ccttcaacgt gccgggcttc gcccctgtac cgtggcaggt cgggcattcc
    16981 accgaatggg ggacctcgat ggttctcgtg accccctgga atgcctcatc aaaggagata
    17041 ttcaggtcat acctgagatc cgcacccggc tgcggcgccc gcgccctctc ccgccccccg
    17101 ccgccgaagc ccgggaagat gctgaagata tccccgaagc cggagtcccg gaagaggtcc
    17161 tcgaaattgg tggtgcggaa gccggcgaag tcctcgggcc ggaaggccgc atggccgaac
    17221 tggtcgtact gggccttctt ctgggggtcg gagagcacct ggaaggcctc gttgatctcc
    17281 ttgaactgct cctcggcccc cttgttcccg gggttcaggt ccgggtggta tttccgggcg
    17341 aggtgcctga aggccttctt gatatcatcc tctgacgcgt tcctggggat ccccaggagc
    17401 tcgtaatagt ccttctttgc catgccccgg cggccggact cccggctagt cctcctttac
    17461 cttgaagtcc gcgtcaaaca ccttctcgtt cccggccggc ccaccctggc cggatgactc
    17521 tcctgaccct tccccggacg gggacccccc cgcccgcgcc ttttcctcgg cctgcttctt
    17581 ctgggcctcc tggtagatgg ccgatcccgc ctctgcaagg accttctgga gggccgcggt
    17641 ctcgtccttc attttctgga tatccttccc ctcgagggct gacctggtgg ccttgacggc
    17701 cgatcctatt ttctccttca ggtctgccgg tatcttttcg ccgagatcgg cgatggtttt
    17761 ctcggctgtg tagatgagcg aatcggcctc gttctttgcc gtcgcctcct ccttccgctt
    17821 ctcatcgtcg gccgcgaact gctgggcctc cttcaccatc cggtccacgt ccctgtcggg
    17881 gagcttcgtc gaggcggtga tggtcatctt ctgctccttc cccgtgccca ggtcctttgc
    17941 cgtgacgttc aggatcccgc tcgcgtcgat gtcgaaggta acctcgatct ggggtacccc
    18001 ccgcggagca ggcgcgatcc cgaccaggtt gaacctcccg aggctgatat tgtcggccgc
    18061 catgggacgc tcgccctgga ggacgtggat ggtgacctcg gactggaggt cggcggctgt
    18121 cgtgaagatc tggctctttc tcgtggggat ggtggtattt ctctcgatga gcgtggtcct
    18181 caccccgccg agggtctcga tgccgagggt gaggggggtg acatcgagga ggaggaggtc
    18241 ggtcacctcg ccggcgagga tcccgccctg gatggccgcg ccgagggcga cgcactccat
    18301 ggggtcgacc ccgcgctcca cttttttccc gacccggtcc tcgatgaact tctggacgat
    18361 gggcatcctc gtggggccgc cgacgagaat caccttgtcg attccctccc tcgtgagctt
    18421 cgcgtctgcg agagccgtgt cgaggggatg cacacaccgg ttgatgatgg gcgtaatgag
    18481 ctgctcgagt ttcgaacggt tcagcttcag ggtgaggtgc ttcggtccct ctttatcggc
    18541 ggtgatgtag ggaaggttga tctcggtctc gacgacggtg gagagctcga tcttcgcctt
    18601 ctcggcggcc tcccggaccc tctgcaccgc catccggtcc ttctttaagt ccaccccttc
    18661 ctgcttccgg aactcctcga cgatccagtc catcacggtc tggtccatgt cggtcccgcc
    18721 gagctgggtg tcgccggagg tggagagaac ggtgaaggtc ccgtggccga actccatgat
    18781 ggtcacgtcc agggtcccgc ccccgagatc aaagacgagg atcttgtgct ccccgccctt
    18841 gtcgaggccg tacgccatgg atgccgccgt gggctcgttc acgagcctga ccacctcgag
    18901 gcccgcgatg gtcccggcat ccttcgtggc cgtccgctgg ttgtcgttga agtaggcggg
    18961 gacggtgatg accgcctttt tcacctcctc cccgaggaag gcctcggcgt ccttccggat
    19021 cttctggagg aggaaggccg agatctgctg gggggtgtag gtcttccccg agatccggta
    19081 caggtgatct gtccccatct tccgcttcgc ggccatgacg gtcccgtcgg ggttgctcac
    19141 ggcctgcctc ctcgccggct cacccaccag gagctccccc tccttcgtga aggccaccac
    19201 cgacgggaac atcttccccg ccacggtcac cccctcggcg gaggggatga tgaccggctt
    19261 tcccccgagc atgacggctg cctggctgtt cgacgtgcct aaatcaattc cgatgatctt
    19321 ttccttcggc atggttctcc tccctggatt ccttcgtgtt ctctgcgatc ttcaccttgg
    19381 atggccgtat caccttcgat ccgagcaggt accccttctg gaactcctca aggataatcc
    19441 cgtcgtcccg gtcgctctcc tcccgggcga tggcctcgtg gagccggggg tcaaagggcc
    19501 tcccgacgct ctcgatgggc gaaagaccgg tgtcctcgag gaacctgcgg agcttcctcg
    19561 acagaagccg gaatccttcc cggttctttt ctccctcgag gcatggcagg gcctggtcca
    19621 gatcgtcgag aaccgggagg aggcccgcca ccagggcttc ccttgcccgc tggatggcga
    19681 ggaccttctc cttctccagg ctcttccggt agttgtcgaa ttcggcctgg aggtactgga
    19741 gctgcgtaag gcgttcctcg gcggtgcgcc tcgcctccgc gagttccccc tcgagtcgtg
    19801 cgatcttcct ttccggatcc tctccctggg gagaaacctc accggtcatc cttcataata
    19861 tcgggcattc catatttata tttttctttc ccaaagggta atccttcggg agcttccgga
    19921 gatccccggc ccggggattc aggctccccc ctcctccgga aggattgccc tcttcgggtc
    19981 aaggaggatg gagttgtcga ggccgatgca ggagaccgcc gcgactgccc cgacgatccc
    20041 ccttccgccc cttgtgaaga tcttgtgctc ccgggcgatc tcctctgcct cctcccgggt
    20101 gacctcgccg gtccgcaccc ggttcccgaa ctgccggaga tcccggggaa cggtgaagcc
    20161 ctgtttgatg gcgatccccc attcgggaga gagggcctcg tccgaaacga accggaggct
    20221 gtgtgcccga atctcggggc agacggcagg ctcggcggcg agctcgatga aactcgccga
    20281 gttccccgcg gtcctccccg ggtgggccgg gttgagcatc acgacatggt gcccgatggg
    20341 catgaccccc ccgatggtcg agaggtgctg gtagagggcg agagcgaggg caaaggtcgc
    20401 acccccctcg ggcgagtcgg tgtcgtcgat acctatggcg aggaagcgca acgcccgggt
    20461 ggtgacctcc ccctcgacga aggcgcccga ccgtgcgacc ttcacgtcct ccaccccctc
    20521 ggcgtgggcc atcatgtcgg tgagggagta cgccggaccc ccgacgcacc ggatgctctg
    20581 gaccaggttc tccccgtccc gccggaggcc ggtgaccccg acggaaggat cgggaaagag
    20641 gccgatggga gcatcccggg acccgaggga ggcccgctcc cggaggaggg tgccgtcccg
    20701 ggagacccgg gagaggatcc cgccggcccg ccggtggtgg aactcgcaga aggctgcgct
    20761 ccccgccgag aggcactcgt ggtagatctc ggctgcatcc ccgtcgatgg tggtgaagat
    20821 ccgccggcag gcgctcgagg gcatcgcgct cttcgagagg taccgggccg gggaacgtcc
    20881 gcgccggcct ttccggacga ccaggcatcc ctctgcgagg agccggttga tccagtcctg
    20941 ggcggtggat cgggggaggc ccgtgctccg ggcgatatcc gaaacggtga agtacccccg
    21001 gtccagagtg aagctccgca tcagcctgag gatatcgcgc cgcctctcaa ggacgcccgg
    21061 catacatctc ccggatggag aggagatccc cgatgaccgc gctcgctgtc tcgatggagc
    21121 cggccccctt cccgatgaag gtgagctccc cggccatgtc ggtgaccacc gtcaccgcgt
    21181 tcagggtccc ctccacgagg aggggatggt tccgggggag gatccggggc gagacccgga
    21241 ggagattcct cgaggggacc gcctccccga tgagccgtat ggcggtctcc ccgtccttgg
    21301 cgagctggat ggcctcggga gtgatccggt cgatccccgt cacctccacg tccttcatgc
    21361 ccgcgtccat ccgccagatg gtattcgcga ggataacgag cttgatggcc gcgtcgaggc
    21421 ccttcacgtc gtacgtgggg tccttctcgg cgtatcccat ctcctgggcc tcgccgaggg
    21481 cctggtcgta cgtgagcccc tcgtcctcca tccgggtcag gatgtagttg caggtcccgt
    21541 tcatcacgcc gaagagggcc gcgaccgtgt tccccaggag gccgtgctgg agggtgtgga
    21601 tgatggggat ggcgccgcag accgtggcct cgtaccggag ggcaaccccc tgcgtcgcgg
    21661 cgaggtcggt gagctcccgg tgggcgaggg cgatggggcc cttgttggag gtaaccacgt
    21721 gccgtcgtcg gttgagggct ccccggatgt gggtgagggc gggctcgccc gtgtccacgt
    21781 tcgtcggggt gacctccacg aggacgtcgt aggcatccga ctcgacgata tcgagggcag
    21841 agaccttcgg cctcccgcac gagcccgagt tctccttccg ggagaaggcc tcgggaatgg
    21901 agatgccctt cggatcgatg atcccgccct tcgagtcggc gagggccgtg agggtgaggg
    21961 ggaggtcctt ctccccgatc atcctcgcca ccccccggcc caccgagcca aaccccagga
    22021 gcgcgaccct catgggccgt cctccagggg ctccacgaca aggatctcct tcctcgccgc
    22081 gacctcccgc aggatgccca cggcgctctc catgtccccc cggttcgcgg cccggatggt
    22141 gatccgggcg gaagagcgct cgctgatccc aggcatcata agggagagct ccgagacttc
    22201 ggcatacccc gtgcggtcga tctggtcgac ggtatcgctg atgtccgtgt gcatgatgtg
    22261 gccgatcatg atgagggccc gctggtggag gaggcgctcc tcgccgagcc tgatgatatc
    22321 caccccgagg gacttgatct ggtcgacgag ggccgggagc cgtcccgagg ggagatcgag
    22381 gacgatctgc acgtcgatgg tgccggactg cgactccgga tcccgctggt gaatcaccgc
    22441 gatgatgttg ccgccgcagg cagatatggg ctcaaggacc ctgacgagct ggcccgggac
    22501 gtccttcatc tccagtttca tggatacctg cacgttcgac cacctggtga tcgttatttg
    22561 aactccctcc ctgataagag tataagaact gtccggagcc ggcacgggag tcgatacctg
    22621 tggggccttg cccgccccct gcgaccgggt gaagccggaa ggagaacgga tgaaggtccg
    22681 cgtcatcgcc tgcgggaacc cgctgatggg aaacgatgcc atcggacctt cggtcatggc
    22741 cctcctcgcg aaggagcatc cggaggtgga cgtcgtggac gggggtaccg gaggactcgg
    22801 gctcatcccc ctgatggagg ggtacgaccg gatcgtgatc gtggatgcca cctcgggcat
    22861 ggggaagatc ggcgatgtcc gggtcatccg ggacattccg gcgggcggga tcgcctccct
    22921 ctccctccac gagctgggga tacccgaggc cctcggactc gcccggaccc tcgggatctc
    22981 cccgggggtg gtcatcgtgg ggatcgaggg cgggacgatc cccgggttct ccggagagat
    23041 ggatccggcg gtcagggccg cgatccccga ggcctgccgc cgggtgctcg aggaactgag
    23101 gaggtgaccg gaaaaaaggt tacttggacc cgggtgcggt ggtgacgatc cggagggcct
    23161 ccccgatctc ggggaggagc tccgccggga tacccatgac catctcgtcg tcggcgatcc
    23221 ccgagaattt ccgggagccg tcgcacccga gggaaaagtt cggggcaccg gtgaggtagg
    23281 gctgggcggt ggcatcggag cagaccgact ggatccccgc catggccggg gagagccggc
    23341 cgccgagccg gaaaatgctt gcctgggcga gcttcagcat gttcctcgga gtggtcacca
    23401 cgaggacgac atcggggtca aagggagtct tctccagggg ggcgtaaatg gtggcatagg
    23461 tctcgcccgt gcggaggtgg gggatcttat ccatggtcct ccggcaggcc gccatgctct
    23521 cgaacttccc gagcttgaag tagaactccc cgctctggag cgtgggggtg atctcccgga
    23581 ggccgagggc ccaggcgccg ccgttgcact ggtgcttcgc cgagggtgca tagaagattg
    23641 ccccctcctt tctcgccata ttcaccatct ggcagtgccg gacggtctcc ttcccctcgg
    23701 ggaccccggc gggcacctcc tcccggctct tcgcgagctt cacggccacg ggtgacccgg
    23761 cgagggagag agctcccttc agggtttcgg caataccggc gtaggagatc ttgggtttcg
    23821 tctctgtcat aaccatcctc tccgaatacg aacacactct ccctgatccc tgataaggct
    23881 tgagatgcgg ggcgggagca gtccccggac ctggaaaatg cgggagggaa cagtccgggg
    23941 tctcggggcc cgggcccgga ttttaagggg gcttgagccc ggatctaggg ccccggcccg
    24001 gttctaaggg ggcgtgggcc cggatttaag ggggctcggg cccggttcta agggggctca
    24061 cagcctccgg agcttcctgt ctaccttgtc gaaaaagttc ttaccgacgt caacgaagag
    24121 tgccgggctt tcggagcggc ggatggtgag gacggccccg agggcgagct ccctcgtctg
    24181 ctgcccgtcg atgacgagcc tcgccggctt ctcgctttcg agggtgatcc ggaggacccg
    24241 gtcgcccgcg atgacgaccg gccgcgagga gagcatgtac ggggcaaggg ggaccaggag
    24301 gagaccctcc acccgggggt cgacgatggg gccgccggcg ctcatggcat aggcggtgga
    24361 gccggtgggg gtggagacga gcatcccgtc ggcccggaag cgctccgcga ggatcccgtc
    24421 gacctcgacg gtgaaccgga gcatcttcgc tggccggctc gtcacgatga ccacctcgtt
    24481 cagggcctca ccaattcccg ccccgtccag ggtgatcccg atccgcatcc tcctctcgac
    24541 ccggaacccc tcccggagcc cctccaggaa tccccttgcc tcaccgggct cgagctcggc
    24601 gaggaagccc acctcccccc agttgatccc gagaatgggg atctgcgact tcaggccctg
    24661 gaccgtgtag aggacagaac cgtccccccc gatgaccacg gcgaggtctg ctccggcctc
    24721 cccgagatcg gtgcccggga cccccaggcg cctggccagg gcatcttcga ggatgacctc
    24781 gtggtgccag tcccggaggt ccccggccag ctgccgggcg aagtcgaccg cccgatggtc
    24841 atccacccgt gaaacgacaa ggatcctcat gctacctcag gtactcgatg accttcccat
    24901 ggagggtccc gttgctcgcg accaggcact tcccgacggt cacctcgtcg gggaaactga
    24961 ttcccctgcc gtcgaggtcc gaaacctttc cccccgcctc ctcgcagacg agcatgcccg
    25021 ccgccgcatc cgtcacccgg agggtcgccc ggaggtcgat gaacccgtcc agcctcccgg
    25081 agccgacgta gcagagctcg agggccgagg ccccgaggag acgccagcgc cggatcttct
    25141 gcccgaggaa gaggacgggg gccgggttga acttccgccc atagacggac atggcgctct
    25201 cctcgaggag catggtccgc gagacccgga tgggcctccc gtcccggaag gccccttttc
    25261 cccgctccgc ggtgaaggtc tcgtggtggg cgaggtcccg cacgaagccc tgccggaccg
    25321 tcccctcctc ggcgtatgcg atggagaggg catagaaggg gatgccggcg agggcgttgt
    25381 gggtaccgtc cacggggtcg aggaagatgg ttcctctctc ccctcccatg ttctcccgct
    25441 tcccgcactc ctcggagacg agggtccggc acagggcatg ggtgccgatg tactcgacga
    25501 tccagtcctc cgccgcctgg tcgatctgcc gggtcggggt tccgctcgca ccggtgcgta
    25561 caacccgtcc tccctcgggc cgtgccacca gatcctggac aatatcctcc accgcacggg
    25621 ccatctcctc gcagcgggcc agaaatgcca tgatctccgc gtggtattta tggtatgcca
    25681 tccagataaa ctacaccaca caagaggata gcgagaatga aggaacagac agaggtcggc
    25741 aagctgaagg aaggcaggta cgtggtcatc gacgaggagc ccagcaagat cctggggatc
    25801 gcgacatcca agcccgggaa gcacggggcg gccaagtccc ggatcgatgc cgtgggactc
    25861 ttcgacggtg tgaagcggtc catcgtccag ccggtctctg ccaagatcta cgtacccatg
    25921 gttgagcgga agggcgccca ggtcatctcc aaggcgggca gggtcatcca gctcatggac
    25981 gtgaaggact acaccatgtt cgaactgacc gtctcggaag aggaagcggc ccggatcgag
    26041 accgggaagg atatcaccta catcgagtcc atgggcaaac ggaaactgga ctgaatcgat
    26101 tcaaaatacc atttttccca tcaggtatac tccaggggca ccttgatcct gatggtatag
    26161 gttttgatga aggatgtatc gacccggaag taatacgtcc tccttccttc gtacagcttc
    26221 tggctccagg gggtgtccga aggttcctcg taaatgtact gccggatggg ggcgatgacg
    26281 agccggttgg ggtcctgggc atcaaagacc tggatgatga gcatcgggta ggcattcggc
    26341 ggcagggcca tgggttctgc cgtgtattcc aggacccacc agggggccgg gacatagaca
    26401 ttctcggtgg tacccggcca ccggttcgat atggtggcat aggtgacgag cgaccggttc
    26461 tggagcgacg gctgggggat ggtggggttg ggtgtgggcg tcacctgtcc ctccggctgg
    26521 cccacgaagc cgacggattt caccgacccg tcccaggtcg gggtcggggc aggggttgtc
    26581 ggaggaaggg tgggcactgt ttttgcggga gtgaccacct gggttaccga cggggtcacg
    26641 gcaaccgtgg gagttgtccg gatacccgtt cccgggccgg tgccggcgat tggtccgacg
    26701 aggaggggcc tcaccagcag ggcaaggacc gcaaaggcca cgatggcgat gacgacaacg
    26761 aggatgtcct gtttctccat acggatccag aggttcgcat aaaatatgga gcaggagagg
    26821 gtaatattct ttccgggccc ggttttcgct gctcaacccc catgacgatg ggaattttta
    26881 cttttgatgt aaatgaagcc ttcccgaaaa caggttgata taaatagtaa aaatttgaat
    26941 tatttctcca tgtcagg
//
LOCUS       DATGDI010000141         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_130508, whole genome shotgun sequence.
ACCESSION   DATGDI010000141 DATGDI010000000
VERSION     DATGDI010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130508"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VKO45_05380"
     CDS             complement(<1..257)
                     /locus_tag="VKO45_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05341.1"
                     /translation="MASAPVRNPLAGFALAIVTAAALSGVAGFASSFIPYLEFRLFFL
                     GVGLCGVGALAGRRTFLGSLGFMGSYLGAFLGLYTAEALFW"
     gene            complement(341..1168)
                     /locus_tag="VKO45_05385"
     CDS             complement(341..1168)
                     /locus_tag="VKO45_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HMA05342.1"
                     /translation="MSNLARFARVVDRPESAINLAEAALLIAEDAYPRLDTDAYLRRM
                     DELAEPLVDRVRPDSSLLETVEALNGHLFGDVGFRGNAQDYYDPRNSYLNEVLDRKLG
                     IPITLSLVYMEVARRLGLTVVGIGLPGHFVVEARQGDSSLLLDPFAGGQVLNLEDCER
                     LVQHVYGDSVEFSEDLLAPVRKRQILMRMLNNLKRNYLTADDAVRAWPVVEKEVFLDP
                     ENAVDRRDRGLLAHRLNQFGLARSDLRFYLERRPDAPDRVAIQASLSAIEAILQMMA"
     gene            1424..>2227
                     /locus_tag="VKO45_05390"
     CDS             1424..>2227
                     /locus_tag="VKO45_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05343.1"
                     /translation="MALTHDDVYGSSGTLPSEETRDEPIVIRKVPTGIADLDSIVDGG
                     FPSGSTVLLRGDIGAGMQEFAYTAASKIAIVNERPDSWHYYLGDHCDTSDLPSKIAYV
                     TFSRSRETILTELATSFNSEYFYAFRDRTIFKDFSSVYYRHSVVPQSWTHQDAFDLPS
                     TNVLEELVTFLEEEGQGAMVVIDSITDLAVSEAVEIRDLVTTVKGLQRAAKKWDGLVY
                     LLLTNGILEPRTEQMLMDSTDGCLVFQWKTSQRSSTRQRYMYLEKFTGVL"
BASE COUNT          408 a          749 c          725 g          345 t
ORIGIN      
        1 atccagaaga gcgcctcggc ggtgtacagg cccaggaagg cgcccaggta ggagcccatg
       61 aagcccaggg aaccgaggaa cgtccggcgt cccgcgagcg ccccgacccc gcacaggccc
      121 acgcccagga agaacagccg gaactcgagg taggggatga acgagcttgc gaatcccgcc
      181 acgccggaga gggcggcggc ggtcacgatg gctagcgcga accccgcgag ggggttccgg
      241 acaggggccg aggccatgca ggtcccaccg gggccgccaa ccccgggttg cgttcaagta
      301 cctgacgtcc tcggtatcgt ccggtgataa tgaggaaagg tcaggccatc atctggagga
      361 tggcctcaat cgcggagagg gatgcctgga tcgcgacgcg gtccggggca tccgggcgac
      421 gctccaggta gaaccgcagg tcgctccgcg cgaggccgaa ctggttcagc cggtgggcca
      481 ggagcccccg gtcccgccgg tccacggcgt tctcggggtc caggaagacc tccttctcca
      541 cgacgggcca cgcacgcacg gcgtcgtccg cggtgaggta gttccgcttg aggttgttca
      601 gcatccgcat gaggatctgc cgcttgcgga ccggggccag gaggtcctcg gagaactcca
      661 cgctgtcccc gtacacgtgc tggacgaggc gctcgcagtc ctccaggttg aggacctggc
      721 caccggcaaa cggatccagc aacagggagg agtccccctg ccgcgcctcg acgacgaagt
      781 gccccgggag gccgatgccc acgacggtga gaccgagacg ccgggcgacc tccatgtaga
      841 ccaagctcag ggtgatcggt atgcccagct tgcggtccaa gacctcgttt aggtaggagt
      901 tccgggggtc gtagtagtcc tgggcattac cccggaaccc cacgtcgccg aagaggtggc
      961 cgttcagcgc ctccacggtc tccaggagcg aggagtccgg gcggacccgg tccacgaggg
     1021 gctcggccag ctcatccatg cgccgcaggt acgcatccgt atccaggcgg ggatacgcgt
     1081 cctcggcgat cagaagcgcg gcctccgcca ggttgatggc ggattccggc cggtccacca
     1141 cacgggcgaa ccgggccaga tttgacaaag gaaccgtccg ctccatccag gcccggcgtc
     1201 ccaaagcagg accccgtcat aaggtttctc ggatacgccc cgcgggatgg ccccattccc
     1261 acgcgcgcgt gcgcacgctg ccgtggagga atatcattcg tattgacgaa tttaagtcgg
     1321 ttcccgctcc ggtcccgacc ggggggtgcg gacgccgcgg cgggccgcga ccctttacaa
     1381 aaagccttta tctcgacgag gcgctccgtc gacctcgagt gggatggctc taacgcacga
     1441 cgacgtctac ggtagctcgg gcactctccc ctccgaggag acgagggacg agcccatcgt
     1501 gatccgcaag gtcccgacgg gcatcgcaga tctggacagc atcgtggacg gcgggttccc
     1561 cagcggctcc acggtcctcc tgcgggggga catcggcgcg ggcatgcaag agttcgccta
     1621 cacggccgcg tccaagattg ccatcgtgaa tgaacgcccc gactcctggc actattacct
     1681 cggggaccac tgcgacacct cggacctgcc ctccaagatc gcctacgtga ccttctcccg
     1741 gtcgcgggag accatcctga cggagctggc cacgtccttc aactcggagt acttctacgc
     1801 cttccgcgac cggaccatct tcaaagactt ctcctccgtc tactaccggc attccgtggt
     1861 cccgcagagc tggacccacc aggacgcctt cgacctaccc tccacgaacg tcctcgagga
     1921 gctggtcacc ttcctcgagg aggagggaca gggggccatg gtggtcatcg actcgatcac
     1981 ggacttggcc gtctccgagg ccgtggagat ccgggacctc gtgaccacgg tcaagggcct
     2041 ccagcgggcc gcgaagaagt gggacggact cgtctacctc ctgctcacga acgggatcct
     2101 ggagccgcgg accgagcaga tgctcatgga ctctacggat ggctgcctcg tcttccaatg
     2161 gaagacgtcc cagcggtcct cgacccggca gcggtacatg tacctggaga agttcacggg
     2221 cgtcctg
//
LOCUS       DATGDI010000142         4531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_130758, whole genome shotgun sequence.
ACCESSION   DATGDI010000142 DATGDI010000000
VERSION     DATGDI010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130758"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..814)
                     /gene="surE"
                     /locus_tag="VKO45_05395"
     CDS             complement(11..814)
                     /gene="surE"
                     /locus_tag="VKO45_05395"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HMA05344.1"
                     /translation="MKPRILLTNDDGIYSAGLWAAYEALSGIAEVTIVAPATQQSAVG
                     RSISIFEPIRVHEVDLQGIRGIAVAGKPTDAVIIGLYSLGLEPNLVVSGINIGENLSF
                     ESIMTSGTVGAALEAANQGTPAIAFSLQVEDQGEKFDDPRCYGKSCTDARRIVLDVTT
                     RVLEQGFPPGADVINVNIPSRIRGGYEVTRLSRKLFHTGVEKRLDPRGRPYFWINGPL
                     VEDAEEGTDVHAIRKGNISVTPITLDCTAHGACAGIRRLFPDSGPAPSA"
     gene            894..1778
                     /locus_tag="VKO45_05400"
     CDS             894..1778
                     /locus_tag="VKO45_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HMA05345.1"
                     /translation="MKVLLAEYATFHDPGLAAEGAAMLRVLSGSFSRIGYEVITPGKG
                     SFDQEIERLAPRCDFGLVIAPDHLLPGYTKILEDLTRNLGCSSLVAAVCANKVMTGEI
                     LRRHGVPVPEKAESGLRVVKPVQGCGSVGVRLTDASPGEGEFAQQFIDGEHLSASMVC
                     SRNTGEACLYYSGKPPLALSLNRQLIDMEKGMFHYLGGETPAEHPKSGEILDVARRAV
                     TVLGCQGYAGVDMVVADRAYVVDVNPRITTSLIGIAAVMEEEVAGILVDASHGGGPDV
                     VHLRGRVRFTKDGQVTRL"
     gene            1775..2713
                     /locus_tag="VKO45_05405"
     CDS             1775..2713
                     /locus_tag="VKO45_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HMA05346.1"
                     /translation="MIGIDVGGANLKMVDEKKVTIRYCPLWEKAPLADLLRAYRDTHP
                     DPRAAVVMSGELADCFASKMEGIRCIVAAVLKVYPEAFFYGTDALFHREEVPQLAAAN
                     WLAAADYLRMSFPGSVLLDVGSTTTDIIPLPLFPRLLGLTDLDRLQRGYLLYTGLLRT
                     SVATQIPSVTLEGVRTPTCAEHFAIAADAHLVLGHISPRAYSCDTPDRGQKNVAAALR
                     RLARVVCADLDEIGPEGALEIARQFWEVQRDRIREATDRVVRDCGANGVVVAGIGSSL
                     LAREIGGRDLAKEMGAVADALPAYAVKEVALREPGS"
     gene            complement(2734..3390)
                     /locus_tag="VKO45_05410"
     CDS             complement(2734..3390)
                     /locus_tag="VKO45_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05347.1"
                     /translation="MRLPAIPPMLGDLIRTTLLAMEGVSFTELAACPTCGGPLRHHDR
                     KEKRFARMRLDGEERDIHVLVARFRCGSCGGFWYAREPFYEGVRFGIPVVDLALTLAR
                     DHPYHRTERILASLGLLVDRGTIRTYAIRAQRPVPATGLLGMPLPISLIRLSNLVSRG
                     PKGAPVPGAEVLAALGLPPAHRAPEHPAPPSQERDERDTEDQEKGGNAKKEEHRRKAE
                     "
     gene            3558..>4531
                     /locus_tag="VKO45_05415"
     CDS             3558..>4531
                     /locus_tag="VKO45_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HMA05348.1"
                     /translation="MDVKYVPTTCPYCGTGCGFNLVVKDGKAVGVSPWHRNPVNDGKL
                     CPKGNYAYEFIHSEDRLMKPLIKKGDKFVEASWDEAYDTIVMAFKKYKPEEMACLSSA
                     RVSNEENYLMQKFARAVLKTPNVDHCARLCHASTVVGLAGTFGSGAMTQSIADIAQAE
                     TLLIIGSNTFEQHPLIGRRVMQAKAKGAKMIYADPRMTATGKQADLHLPFFSGTDVAL
                     LNCFMQQILKNGWENKEFIEKRTKDFDKLKAVVMKPEYSLENVAKITGVPAKDIATAA
                     EWIGKSKANAILYSMGITQHTTGVDNVKSVANLQMLTGNLGRPGTGVCA"
BASE COUNT          822 a         1417 c         1509 g          783 t
ORIGIN      
        1 cgaccgccca tcaggcggac ggggccggac cggaatcggg gaaaagacgc ctgataccgg
       61 cgcaggcacc atgggccgta cagtccaggg tgatgggcgt cacggaaatg ttgcccttcc
      121 ggatcgcgtg gacatcggtc ccctcctcgg cgtcctcgac gaggggaccg ttgatccaga
      181 agtagggtct tccccggggg tccagccgtt tctcgacccc ggtatggaag agtttccgtg
      241 agaggcgcgt cacctcgtac cctccccgga tccgggaggg gatgttcacg ttgatcacgt
      301 cggccccggg ggggaagccc tgttccagca cccgggtcgt gacatcgagg acgatccgcc
      361 gggcatccgt gcagctcttc ccgtaacacc gagggtcatc gaacttctcc ccctggtcct
      421 cgacctggag ggagaaggca atggccggcg tcccctggtt cgcggcctcg agggctgcgc
      481 ccaccgtccc cgaggtcatg atggactcga aggagaggtt ttccccgata ttgatacccg
      541 agaccacgag gttgggctcg agcccgagag aatacaggcc gatgatcacg gcatccgtgg
      601 gcttcccggc gacggcgatt ccccggatcc cctgcaggtc aacctcgtgg acccggatgg
      661 gttcgaagat ggagatggac cgcccgaccg cgctctgctg ggtggccggt gccacgatgg
      721 tcacctcggc gatgccggag agggcctcgt aggccgccca gagccccgcc gagtagatgc
      781 catcgtcgtt ggtgaggagg atccggggtt tcatccagat cgtagttgga gaggggggac
      841 caagaaggaa tcgatcgtca tggtaaaggg taatcggtgc acaccggtac tggatgaagg
      901 tcctcctcgc cgagtatgcc acctttcacg accctggcct tgcggccgag ggggcagcga
      961 tgctgcgggt gctctcgggc tccttctccc ggatcgggta cgaggtgatc acgcccggga
     1021 agggttcctt cgaccaggag atagagcgtc tggccccccg gtgtgacttc ggcctggtca
     1081 tcgctccgga ccacctcctc ccgggataca cgaagatcct cgaggacctc acccggaacc
     1141 tcgggtgcag cagcctcgtg gccgcggtct gcgcgaacaa ggtgatgacc ggggagatac
     1201 tcaggaggca cggggtgccg gtacccgaaa aggccgaatc ggggctcagg gtggtgaagc
     1261 cggtgcaggg gtgcgggtcg gtgggcgtcc ggctcacaga tgcatccccg ggagaggggg
     1321 agttcgcgca gcagtttatt gacggagagc acctctcggc gagcatggtc tgttcgcgga
     1381 acacgggtga ggcctgcctc tactactcgg ggaagccccc cctcgccctc tccctgaacc
     1441 gccagctcat cgacatggaa aaaggcatgt tccattacct cggcggcgag acccccgccg
     1501 aacacccgaa gagcggggag atcctcgacg tcgcccggag ggccgtgacg gtcctcggct
     1561 gccaggggta tgccggcgtg gacatggtgg tcgcggaccg ggcctacgtg gtggacgtga
     1621 acccccggat cacgacctcc ctcatcggca tcgccgcagt gatggaggag gaggtcgccg
     1681 ggatcctcgt cgatgcctcc catgggggcg gcccggatgt ggtccacctc cggggaaggg
     1741 tcaggttcac gaaggacggg caggtgaccc ggctgtgatc ggcatcgatg tggggggtgc
     1801 aaacctgaag atggtcgacg aaaaaaaggt caccatccgg tactgccccc tctgggagaa
     1861 ggcccccctc gcggacctgc tccgggctta ccgggatacc catccggatc cccgggccgc
     1921 cgtcgtgatg agcggtgagc tcgccgactg cttcgcttcg aagatggagg gcatccggtg
     1981 tatcgtggcg gccgtcctca aggtgtaccc ggaggcattc ttctacggga cggacgccct
     2041 gttccatcgg gaagaggttc cacagctcgc ggcggccaac tggctcgccg cagccgacta
     2101 cctccggatg tccttccctg gctccgtcct cctcgatgtc gggtcgacca ccacggatat
     2161 catccccctt cccctgtttc cccggctcct cggtctcacc gacctcgaca ggctccagcg
     2221 tggctatctt ctctacacgg gccttctccg aacctccgtc gccacccaga tcccatctgt
     2281 gacgctggag ggggtcagga caccgacctg tgccgaacac ttcgccatcg ccgcggatgc
     2341 ccaccttgtc ctggggcaca tttccccccg ggcgtacagc tgcgacacac cggaccgggg
     2401 tcagaagaat gtggccgcgg ccctccgcag gcttgcgagg gtggtctgtg cagatctgga
     2461 cgagatcggt cccgaagggg cactggagat cgcccggcag ttctgggagg tgcagcggga
     2521 ccggatcagg gaggccacgg acagggtggt ccgtgactgc ggtgcgaatg gggtggtggt
     2581 ggcggggatc gggtcctccc tcctcgcccg ggagataggg ggaagggacc tcgcgaagga
     2641 gatgggggcg gttgcagatg cgcttcccgc ctatgccgtg aaggaggtgg ccttacgaga
     2701 acctggatcc tgaccgcggg cctcctcgcc ggctcactcg gcctttctgc ggtgctcttc
     2761 cttcttggcg ttcccgcctt tttcctggtc ctctgtatcc cgctcatccc gttcctggga
     2821 gggaggcgca gggtgctccg gtgcccggtg tgcgggcggg aggcccaggg cggcgagaac
     2881 ttctgccccc gggacgggag cccccttcgg cccccgggag acgaggtttg agagccggat
     2941 gagggagata gggaggggca tcccgaggag accggtggca gggaccggcc tctgcgcccg
     3001 gatggcgtac gtcctgatgg tcccccggtc cacgagaagg ccgagggagg cgaggatccg
     3061 ttccgtgcga tggtagggat gatcccgcgc gagggtgagg gcgagatcca cgacgggaat
     3121 cccgaaccgg accccttcgt aaaagggttc ccgggcatac cagaatcccc cgcaggaccc
     3181 gcaccggaac cgggcaacga ggacatggat gtccctctcc tccccgtcga gccgcatgcg
     3241 ggcgaaccgc ttctccttcc ggtcatggtg cctgagcggg cctccgcacg tgggacatgc
     3301 ggcgagttcc gtgaaggaga ccccctccat ggcgaggagg gtcgtccgga tgaggtcccc
     3361 gagcatgggg gggatggcgg gaagcctcat gcgcacctcg gccttccggg taaattaaat
     3421 gggctgaata tttccgtgaa tacaccgaaa ctctttatca tctcgggccc cacctccacc
     3481 actaataatc agtttttgag gaaaaaacac tatcgttata tgctcgcgca ttgacctttt
     3541 cctgtccgag gttttctatg gacgtcaagt atgttccaac gacgtgtccg tactgcggta
     3601 ccggatgcgg gttcaacctc gtggtgaagg acgggaaggc agtgggagtc tccccctggc
     3661 accggaaccc cgtgaacgac gggaagctgt gccccaaggg gaactatgcc tacgagttca
     3721 ttcacagcga ggaccggctc atgaagcccc tcatcaagaa gggggacaag ttcgtcgagg
     3781 caagctggga tgaggcctat gataccatcg tgatggcctt caagaagtac aagcccgagg
     3841 agatggcatg cctctcttct gcgagggtct ccaacgagga gaactacctc atgcagaagt
     3901 tcgcgcgggc ggtcctaaag accccgaacg tggaccactg cgcacggctc tgccatgcct
     3961 ccacggtcgt ggggctcgcc ggtaccttcg gctcgggtgc gatgacccag tccatcgcgg
     4021 acatcgccca ggccgagacc ctcctcatca tcgggagtaa cacctttgag cagcaccccc
     4081 tgatcggccg gcgcgtcatg caggccaagg caaagggggc gaagatgatc tatgccgacc
     4141 cgcgcatgac cgcgacgggg aagcaggccg acctgcacct gcccttcttc tcgggcaccg
     4201 atgtcgcgct cctcaactgc ttcatgcagc agatcctgaa gaacggctgg gagaacaagg
     4261 agttcatcga gaagcggacg aaggacttcg acaagctgaa ggccgtcgtc atgaagcccg
     4321 agtactccct ggagaatgtg gcgaagatca cgggagtccc ggcgaaggac atcgccaccg
     4381 ctgccgagtg gatcgggaag agcaaggcga acgccatcct ctactccatg ggcatcaccc
     4441 agcacaccac cggcgtggac aacgtgaagt cggtcgcaaa cctccagatg ctcacgggca
     4501 acctgggaag gcccgggacc ggcgtctgcg c
//
LOCUS       DATGDI010000143         5356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_160036, whole genome shotgun sequence.
ACCESSION   DATGDI010000143 DATGDI010000000
VERSION     DATGDI010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160036"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VKO45_05420"
     CDS             complement(<1..380)
                     /locus_tag="VKO45_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F430 synthase"
                     /protein_id="HMA05349.1"
                     /translation="MEILVLDTIHGGTELAAELERSGHRVDTVDVYRGERGISPDDAR
                     ERRYDLLVAPVHLDPDYPLLSAVQAPRITHHLAVRMLLSGKAPRPMIEVTGARGKTTT
                     AHAIAHGMGGTGLPLQRGPISPGRS"
     gene            complement(389..793)
                     /gene="cfbA"
                     /locus_tag="VKO45_05425"
     CDS             complement(389..793)
                     /gene="cfbA"
                     /locus_tag="VKO45_05425"
                     /EC_number="4.99.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin nickelochelatase"
                     /protein_id="HMA05350.1"
                     /translation="MSKKGLLLVGHGSSLPYNKQLVEDTARMVSRRDPGYLVKCAFMN
                     LNTPTIGEALEEFKREDVDTVIVVPLFLARGVHINRDIPEVLGLPEGSYRGSLQKNGS
                     SIPLIYADPIGSDPLLAELMLKNAARALRERL"
     gene            1175..1633
                     /locus_tag="VKO45_05430"
     CDS             1175..1633
                     /locus_tag="VKO45_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase"
                     /protein_id="HMA05351.1"
                     /translation="MSDFEREMVSCLNRFFKAEKRQGFAYRLKQSKFSTQYVDVLVDS
                     LDPRYYCAIECKSIRGKKLYFSQHFHTDRNHVHQIDAISGFLARTGRRGYLAIEFRFG
                     SGRTREAYLIPWEALVGFYAGAPGIGIDDFRSFIALERTPEGYVLRELDR"
     gene            1669..2796
                     /locus_tag="VKO45_05435"
     CDS             1669..2796
                     /locus_tag="VKO45_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 9 domain-containing
                     protein"
                     /protein_id="HMA05352.1"
                     /translation="MSPPADPFDLSVDGHRVKTPIAIASMAGTVDAAYIAARAGHVGM
                     GFIGGYSIDTPTIAASRRMAEAGRCEFLPGDPVAEIGRQADLLAGIPVVGGVNLRAAT
                     PEALSGVARAVGDRLIYEIDAHCRQEPMIEAGSGEFLLRHPDRLIGMVSALKSEGVLV
                     SVKIRAGVAESDPKLARTLWEEGADILHVDLMDAGYAKVRQIRNASPILIIANNSMQS
                     FDRVMEMFSHGADLISLARHSDSQTLAGLDAAITRYANEFGWYNAPKQLCRGGDVRAL
                     TFCCLPVKDCPLQPTLSKVGLSRDEYVDFKKEAVRGTPLQDGQYTCFGSLAWCCKASS
                     PCLLRDATLEQMGMSKKEYMQWKRELSRAIMARVFPSRARL"
     gene            2793..3620
                     /locus_tag="VKO45_05440"
     CDS             2793..3620
                     /locus_tag="VKO45_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HMA05353.1"
                     /translation="MTPVERAQMAMMLEVTAYPKPGNVDRCHDYEGTRLEHFLASSIL
                     ARPALERAAGCGGGIGEILRQAVRLTTSHSGGNTHFGAFILLVPLLRGVDIPGAMEAV
                     RGTTVEDSLEFYRAFSETKVRVLPGDPLDITDPHALDTIREKGLTLLDIMIHSMEHDM
                     VAREWVTGFPLTRKAADLLHRSGCGRQGLVDTFLGLLSTETDTFIVKEHGETVAGEVR
                     RRAGEVLAGRENLEVFDADCIARGINPGSTADILIAGIYIALGEGWEWDCSPKREAG"
     gene            3643..4233
                     /locus_tag="VKO45_05445"
     CDS             3643..4233
                     /locus_tag="VKO45_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HMA05354.1"
                     /translation="MGLLREGINEVIATTWNNAAPMGIIFRDGEYRMVLFRGSHTAMN
                     IERWGWVVANIVHDPVLWVRTAFSDLPPSAFVEETVEDFLVHRLPDVEAWIAFDAAIE
                     HRARESMTAILSPMKEVVVTPVVHPVNRGYNSVIEAAVHGTRLLWNKDPKLRELIDHH
                     RSIIRRCGGPREIEALEMLERYLERPKALSSGGNPM"
     gene            complement(4322..4711)
                     /locus_tag="VKO45_05450"
     CDS             complement(4322..4711)
                     /locus_tag="VKO45_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HMA05355.1"
                     /translation="MFRKKREEAVSPVIGVILMVAITVILAAVIAAFVFGMAGGVSKT
                     KSVAATARQSNATACIVTWQGGQDNQAVKSYNVTIGDATTPQGNAISNTVGYSSVYNV
                     CSSAQDHVVVVAAFNDGTSQVVLDTYV"
     gene            complement(4754..5143)
                     /locus_tag="VKO45_05455"
     CDS             complement(4754..5143)
                     /locus_tag="VKO45_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HMA05356.1"
                     /translation="MFRKKRDEAVSPVIGVILMVAITVILAAVIAAFVFGMASGVSKT
                     KSVAATARQSNASACVVTWQGGQDNALVSSYNITFIDAALPQNNAVAPTVGLTTAFNG
                     CSSGQDHVVVTAAFTDGSQQVVLDTYV"
BASE COUNT          997 a         1654 c         1709 g          996 t
ORIGIN      
        1 gagcgtccag gtgagatggg gcccctctgg agtgggagcc ccgtgccccc catcccgtgg
       61 gcgatggcat gggccgtggt ggtctttccc cgggctcccg tcacctcgat catcggccgg
      121 ggggctttac ccgagaggag catccggacg gcgaggtgat gcgtgatccg gggtgcctgg
      181 accgcggaga ggagcgggta atccgggtcc aggtggacgg gcgcgaccag gagatcgtac
      241 cggcgttccc gggcatcgtc gggtgatatc ccccgctccc ctcggtagac gtcgaccgta
      301 tcgacccggt ggccggacct ctccagttcg gccgcgagct cggttccgcc gtggatggta
      361 tcgaggacga ggatctccat ggaacggtct agaggcgctc cctgagggca cgcgctgcgt
      421 tcttgagcat gagctcggcg aggagggggt cgctcccgat gggatctgcg tagatgaggg
      481 ggatggagga gccgttcttc tggaggcttc cccggtacga gccctcgggc aggccgagca
      541 cctcggggat gtccctgttg atgtggaccc cccgggcgag gaagaggggc accacgatca
      601 cggtgtcgac atcctccctc ttgaattcct ccagggcctc tccgatggtg ggcgtgttca
      661 ggttcatgaa ggcgcacttc acgaggtagc ccggatcccg ccgtgacacc atccgcgcgg
      721 tgtcctcgac gagctgcttg ttgtagggaa gcgagctccc gtggcccacc agaagaaggc
      781 cctttttgct catgaggtaa cctgtatacc cgcatgggta aaatgattat cgatgtggaa
      841 cgaggaaaac cctgcatatt ccccgccgtc gtcccgaaaa gggcggggat ggtctcctcg
      901 ggaaatgctg gccggttccc tcccgatgtt ccggaccgtg ggggaacaat cgcttcggga
      961 aggtcgcccc ttccctcccg atgttccgga ctgtggggga acaatcgctt cgggaaatgc
     1021 cggccgtttc ccgtctgcca cccatgacat gagggaaacg tcggttcagg aataggatgg
     1081 gataatccta tgtgagacca ccccccatac tgtaacaggg attgatccgt gaccgggggc
     1141 tggatcgaag gatgatcgga caggaagggg gaatttgagc gactttgagc gggagatggt
     1201 ctcctgcctg aaccggttct tcaaggcaga gaagaggcag ggtttcgcct accggctcaa
     1261 gcagtccaag ttctccaccc agtacgtcga cgtcctcgtg gactccctgg acccccggta
     1321 ctactgcgcc atcgagtgca agtccatccg ggggaagaag ctctacttct cccagcactt
     1381 ccacaccgac cggaaccatg tgcaccagat cgatgccatc agcggcttcc ttgcccggac
     1441 gggccggcgg ggctacctgg ccatcgagtt ccgcttcggc tcggggagga cccgggaggc
     1501 ctacctcatc ccgtgggagg ccctggtcgg gttctacgca ggggccccgg gcatcggaat
     1561 cgacgacttc cggtccttca tcgccctgga acgcacgccg gagggctatg tgctccgtga
     1621 gctggataga taagcctaaa ggctccagag ggaaacaggt tagaatccat gtcacccccg
     1681 gctgacccgt tcgacctctc cgtggacggg cacagggtga agacccccat cgccatcgcg
     1741 tccatggccg gtaccgtcga tgccgcctac atcgcggccc gggccgggca tgtcgggatg
     1801 ggattcatcg gcggatactc catcgacacg ccgaccatcg ccgcgagccg gaggatggca
     1861 gaggccggga ggtgcgagtt cctccccggc gacccggtgg ccgagatcgg gcggcaggcg
     1921 gacctccttg ccgggattcc cgtcgttggc ggggtgaacc tccgggcggc caccccggag
     1981 gccctctccg gggttgcgag ggccgtcggg gaccggctga tctacgagat cgacgcccat
     2041 tgccggcagg agcccatgat cgaggcggga tccggggagt tcctcctccg tcacccggac
     2101 cgcctcatcg ggatggtctc ggccctcaaa tccgaggggg tcctcgtgtc ggtgaagatc
     2161 cgggccggcg tggccgagag cgacccgaag cttgcccgaa cgttgtggga ggagggtgcc
     2221 gatatcctgc acgtggatct catggacgcg ggctatgcca aggtccggca gatccggaac
     2281 gcctccccca tcctgatcat cgcgaacaac tccatgcagt ccttcgaccg ggtcatggag
     2341 atgttctccc acggggcgga tctcatttcc ctcgcccgcc actcggatag ccagaccctt
     2401 gcgggccttg atgccgccat tacccggtat gcgaacgagt tcggctggta caacgcaccc
     2461 aagcagctct gccggggcgg ggatgtccgg gccctcacct tctgctgcct cccggtgaag
     2521 gactgccccc tccagccgac gctctcgaag gtgggcctct cccgggatga gtacgtggat
     2581 ttcaagaagg aggcggtccg gggaaccccg ctccaggacg ggcagtacac ctgcttcggc
     2641 tccctcgcct ggtgctgcaa ggcgagctcc ccctgcctcc tccgggacgc caccctcgag
     2701 cagatgggga tgtcgaagaa ggagtacatg cagtggaagc gggagctctc ccgggccatc
     2761 atggcccggg tctttccgtc gcgggccagg ctatgacgcc ggttgagcgg gcccagatgg
     2821 ccatgatgct cgaggttacc gcgtacccga agccggggaa cgtggaccgg tgccacgact
     2881 acgagggcac ccggctcgag cacttcctcg cctcctccat cctcgcccgc ccggccctcg
     2941 agagggcagc ggggtgcggc ggagggatcg gggagattct ccgccaggcg gtgcggctca
     3001 ccacctccca ctccgggggt aacacccact tcggggcctt catccttctc gtccccctcc
     3061 tccggggggt cgacatcccg ggggcgatgg aagctgtcag gggcaccacg gtggaggact
     3121 ccctcgagtt ctaccgggcc ttctccgaga cgaaggtgcg ggtactcccg ggagaccccc
     3181 tggatatcac ggacccccat gccctcgaca ccatccggga gaaggggctc accctccttg
     3241 acatcatgat ccactcgatg gagcacgaca tggtcgcccg ggagtgggtg accgggttcc
     3301 ccctcacgag gaaggccgcg gacctcctcc accggtccgg ctgcggccgg cagggcctcg
     3361 tcgatacctt ccttgggctc ctctccaccg agaccgacac cttcatcgtg aaagagcacg
     3421 gggaaaccgt cgcaggagag gtgaggagac gggcgggcga ggtcctcgcc gggagggaga
     3481 atctcgaggt gttcgacgcc gactgcatcg cccggggcat caacccgggc tccaccgccg
     3541 acatcctcat cgcagggatc tacatcgccc tcggggaggg ttgggaatgg gactgttccc
     3601 cgaagagaga ggcgggatga ttcccacgag gggggcctga tcatgggact gctccgggaa
     3661 ggcataaacg aggtcatcgc gaccacctgg aataacgccg cccccatggg gatcatcttc
     3721 cgggacgggg aataccggat ggtgctcttc cggggaagcc acacggcgat gaacatcgag
     3781 cgttggggat gggtcgttgc gaacatcgtc cacgacccgg ttctctgggt ccgcaccgcg
     3841 ttttccgatc tccccccgtc agccttcgta gaagagaccg tcgaggactt ccttgttcac
     3901 cggctccccg acgtcgaggc ctggatcgcc ttcgatgccg ccatcgagca ccgtgcacgg
     3961 gagtcgatga cggcgatcct cagtccgatg aaggaggtcg tcgtcacgcc ggtagtccat
     4021 cccgtgaacc ggggctacaa cagcgtgatc gaggcggcag tgcacgggac ccgccttctt
     4081 tggaataagg acccgaaact ccgtgaactc atcgatcacc accggagcat catccgcagg
     4141 tgtggcggac cgagggagat cgaggcgctg gagatgctgg agaggtacct ggaaagacct
     4201 aaagccttat cgtcaggcgg aaatcctatg taatccgaaa tgccctcggg aaaacacgat
     4261 ctcaacgaat acaaagaatt ttcttatact ggatccgttt cgatgggaaa aaaaggtgag
     4321 attagacgta ggtgtccagg acaacctgcg acgttccatc gttgaaggct gcaacgacaa
     4381 cgacgtgatc ctgggctgaa ctgcaaacgt tgtaaacgct tgagtacccc acggtgtttg
     4441 atatggcgtt cccctgaggc gtcgtagcgt cgccaatggt gacgttataa gacttcacgg
     4501 cttgattatc ctggccaccc tgccacgtga cgatgcaggc cgttgcattt gactgcctag
     4561 cggtcgctgc cactgacttc gtctttgaaa cgcccccggc cataccaaag acaaatgcgg
     4621 cgatgaccgc ggcgaggatc accgtgatgg cgaccatgag gatgacgccg attaccggtg
     4681 ataccgcctc ttctctcttc tttctgaaca taatttcacc tgatccagca tgtcttgatc
     4741 ggacgtgctt tgatcagacg taggtgtcca gaacgacctg ctgagatccg tcggtaaacg
     4801 cagcagtaac gacaacgtgg tcctggccgc ttgaacaccc gttgaaggcg gttgtgagac
     4861 ccacggtagg cgccacagcg ttgttctgcg ggagtgcagc atcgatgaag gtgatgttgt
     4921 agctcgacac cagtgcgttg tcctgaccac cctgccatgt gacgacgcag gctgatgcat
     4981 tactctgccg ggcggtcgcc gccaccgact tcgtctttga aacgccgctc gccatgccga
     5041 atacgaaggc ggcaatgacc gcggcaagga tcaccgtgat ggcgaccatg aggatgacgc
     5101 cgattactgg tgataccgcc tcatctctct tctttctgaa cataatttca cctgatccag
     5161 cctgtcttga tcagacgtgc tttgatcaga cgtaggtgtc cagcacgacc tgctgagatc
     5221 cgtcggtaaa cgcagcagta acgacaacgt ggtcctggcc gcttgaacac ccgttgaagg
     5281 cagttgtgag acccacggta ggcgccacag cgttgttctg cgggagtgca gcatcgatga
     5341 aggtgatgtt gtagct
//
LOCUS       DATGDI010000144         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_130840, whole genome shotgun sequence.
ACCESSION   DATGDI010000144 DATGDI010000000
VERSION     DATGDI010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130840"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2632
                     /locus_tag="VKO45_05460"
     CDS             <1..>2632
                     /locus_tag="VKO45_05460"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HMA05357.1"
                     /translation="GDCDDNDPEVYPGFPETCDQKDNDCDGQVDEGFDRDGDGFPGWY
                     SGCGNDCDDADPLTYPGAPEVCDGKDNDCDGETDEGVETAFYRDEDGDGLGGTGTILA
                     CSLPSGASAGTGDCDDRDPARSPGTTEICDGKDNDCDGQVDEDLPLVTFYRDADGGGF
                     GGAETVQACSAPAGYVDVPGDCDDADPGIHPGAEEVCDGKDNNCDGEIDEFLLSTFFR
                     DADGDGFGGAETVRACSAPAGYVDVPGDCDDASAAVSPAVTEVCDGLDNDCDGLIDED
                     LALLTLYRDADADGFGGTMALETCSATLPGYIADSTDCDDTDLFTHPGATEICDGKDN
                     NCDGQVDEGLLLTFYQDADGDGYGGTVTTTACTAPQGHVANSRDCNDADAGVHPGATE
                     IPCDGIDQNCNGAADDGLTDADRDGWTECAGDCNDSNPNINPGVVETGTTLCDGIDQD
                     CDGKDDCSARFVASGGAVLDTETGLSWLSNGMCLGPGRYVRGGTWDTVPSLTERVSLL
                     GDGQCGLSDGSGPGNWRVSTIGEIQNLFRHGYCLDEQLGDQFYYGTPDTLCLGNAAGT
                     YRWKPGDAFADLLYLGKYLTEPGKHWYYYYTSESAGAGYQSGVILYNTTMCRISGSSQ
                     ECRIYVTFAYASTFGTTWDGGFVWPVRDKLVDNDGDGYYRGLDCDDTDPAIHPGATEV
                     CDGIDNNCDGRIDEVCDSDGDGYTPEGGDCNDNDATIHPGATEVCDGKDNDCDGQVDE
                     GVTQTFYRDGDGDGYGGTATILACIAPAGYVANSGDCNDNDAGVNPAAPDPCDGRDTN
                     CDGREENRHVYFRDQDGDGFGALPATLACSAPTGYVDDRKDCDDTNPAINPLGTEVCD
                     GKDNDCDGQVDEGLRQTFYWD"
BASE COUNT          519 a          794 c          907 g          412 t
ORIGIN      
        1 ggggactgcg acgacaacga ccccgaggtc tatcccgggt ttcccgagac gtgcgaccag
       61 aaggacaatg attgcgacgg ccaggtggac gagggtttcg acagggacgg ggacgggttc
      121 ccgggctggt acagcgggtg cgggaacgac tgcgacgatg ccgatcccct gacctacccc
      181 ggcgcgcccg aggtctgcga tgggaaggac aacgactgcg acggggaaac cgacgaggga
      241 gtagagaccg ccttttaccg ggacgaggac ggggacgggc tcggcgggac cggtaccatc
      301 cttgcctgct ccctcccctc gggggcttcg gccgggaccg gggactgcga tgatagagac
      361 cccgcgagat ctcccggaac cacggagatc tgcgacggga aggataacga ctgcgatggg
      421 caggtggatg aggatcttcc cctggtgacc ttctaccggg atgccgacgg aggcggcttc
      481 gggggtgcgg aaacggtcca ggcctgctcg gccccggccg ggtacgtgga cgtcccaggt
      541 gactgtgatg atgcggaccc gggaatccat ccgggtgcag aggaggtctg cgacgggaag
      601 gacaacaact gcgatggtga gatcgacgag ttcctcctgt ccaccttctt ccgcgatgcc
      661 gacggcgacg gcttcggggg cgcggaaacg gtccgggcct gttcggcccc ggcagggtac
      721 gtggatgtcc ccggtgactg cgacgatgcc agtgcggcgg tcagtcctgc cgtgaccgag
      781 gtctgcgacg ggcttgataa tgactgcgac ggcctgatcg acgaagacct cgccctcctg
      841 accctgtacc gggacgccga cgccgacggc ttcggtggaa ccatggccct cgagacctgt
      901 tcggcaaccc ttcccggtta catcgcagac tccacagact gcgatgacac ggacctgttc
      961 acccacccgg gcgcgaccga gatctgcgac gggaaagaca ataactgcga cgggcaggtg
     1021 gacgagggtc tcctcctgac cttctaccag gatgccgacg gcgatggcta tgggggaaca
     1081 gtcacaacca cggcctgcac ggccccgcag ggccatgtcg caaactccag ggactgcaac
     1141 gacgcagatg cgggtgtcca ccccggggcg accgagatcc cctgtgacgg tatagaccag
     1201 aactgcaacg gcgccgccga tgatggcctc acggatgccg acagggacgg gtggacggag
     1261 tgcgcaggag actgtaatga ttcgaatccg aacatcaacc ccggggttgt tgaaaccggc
     1321 accaccctct gcgacggcat cgaccaggac tgcgatggga aggacgactg cagtgcccgg
     1381 ttcgtcgctt ccggtggggc ggtcttggac accgagaccg ggctctcctg gctgagcaat
     1441 ggcatgtgcc tgggacccgg gcgttacgtc aggggaggta catgggatac tgttccatcc
     1501 ctgaccgagc gggtaagcct gctgggggac gggcagtgcg ggctctccga cggttccggg
     1561 ccgggtaact ggagggtttc cactattgga gaaatccaga acctgttcag gcacgggtac
     1621 tgcctggacg agcagctggg tgaccagttc tattacggga cccccgatac tctctgcctt
     1681 ggcaatgccg caggtacata tcgctggaaa cccggtgatg ccttcgccga cctgctctac
     1741 ctggggaagt acctgaccga gccgggtaaa cactggtatt attactacac cagcgagagc
     1801 gcaggtgcgg gataccagtc cggggtgatc ctctacaaca ccaccatgtg ccggatttca
     1861 ggcagctccc aggagtgcag gatttacgtg accttcgcgt atgcctcgac ctttggcacg
     1921 acctgggacg gcgggttcgt ctggcccgtg cgggacaagc tcgtcgataa cgacggcgac
     1981 gggtattacc gcggcctgga ctgcgacgac acggatccgg ccatccatcc gggcgccacg
     2041 gaggtctgcg acgggatcga caacaactgc gacggacgaa tcgacgaggt ctgtgattcc
     2101 gacggggacg gctacacccc ggagggaggg gactgcaacg acaacgatgc caccatccac
     2161 ccgggcgcca cggaggtctg cgacgggaag gacaacgact gcgacgggca ggtcgatgag
     2221 ggcgtgaccc agaccttcta ccgggacggg gacggcgacg gctatggcgg gacagccacc
     2281 atcctggcct gcatcgcgcc cgccggctac gtggcaaact cgggggactg caatgacaac
     2341 gacgccgggg tgaaccccgc tgcaccggat ccctgcgatg ggagggatac gaactgcgac
     2401 ggcagggagg agaacaggca tgtctacttc agggaccagg acggtgatgg gttcggcgcg
     2461 ttgcctgcga cccttgcctg ctcggctccc accggatacg tagacgacag gaaggactgc
     2521 gatgatacca acccggccat caatcccctt ggcaccgagg tctgcgacgg gaaggacaac
     2581 gactgcgacg ggcaggtgga cgaggggctc cggcagacct tttactggga cc
//
LOCUS       DATGDI010000145         6829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_218343, whole genome shotgun sequence.
ACCESSION   DATGDI010000145 DATGDI010000000
VERSION     DATGDI010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218343"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VKO45_05465"
     CDS             <1..538
                     /locus_tag="VKO45_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMA05358.1"
                     /translation="HDDHLGEVIRLGRKTVAISEIGKFLRSKGLPVETMNIGGTITVD
                     GVEFTMTQAIHSSGLEAGGPGFYGGCPCGFVIGMDGVRVYHAGDTALFSDMKLIGELY
                     APDIALLPIGGKFTMGPREAMMAANYVGAKTVIPMHYSTWPAILQDPLSFKHAIERTT
                     DLSVVILSPGEAYEVPAR"
     gene            complement(807..1217)
                     /locus_tag="VKO45_05470"
     CDS             complement(807..1217)
                     /locus_tag="VKO45_05470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HMA05359.1"
                     /translation="MAIPETEPGCPFCNPDEKEVLLRNELAYARTDRFPVSRGHMLVI
                     PFRHVVSFFDTTPEERSAILDLVIRVRGVVEERHHPDGYNIGVNVGRYAGQAVMHVHF
                     HVIPRYKGDAKGHHGGIRWVIPRGPPPAEKNWFG"
     gene            complement(1288..1437)
                     /locus_tag="VKO45_05475"
     CDS             complement(1288..1437)
                     /locus_tag="VKO45_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05360.1"
                     /translation="MLSERQKQSYHRVFRKTCPVCGAEFETFNAKLKYDSHICSRRAK
                     KGKQL"
     gene            1528..2226
                     /locus_tag="VKO45_05480"
     CDS             1528..2226
                     /locus_tag="VKO45_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HMA05361.1"
                     /translation="MSGVYAGDAMAGPTQYSAVNLFGEARVDPARWNRTPSEEEVAGA
                     VTAIGKRGIRVIRARDGREALDALVGLIPRGSEVMNGSSTTLIEIGYESLLRSGKTGW
                     IDLHDRITAENDDRKRAELRRRSVAAEYFVSGANAITQTGEILACDASGSRVGAWPFA
                     AGHLILVAGINKIVPTLEDALRRVREYAFPLENARALRVYGTPSMIGKCVILANEKAE
                     GRVTLILVGESLGY"
     gene            2229..2525
                     /locus_tag="VKO45_05485"
     CDS             2229..2525
                     /locus_tag="VKO45_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05362.1"
                     /translation="MPENPLEHLKALIGRYTVITSIPDIDRYRRITAPKEGHCIFRGC
                     PEPIAWEDAQGGNFLCEGHYRTMKTWIEEARKGLLPGGQLSPLFTQGGTDATRL"
     gene            2509..3876
                     /gene="merA"
                     /locus_tag="VKO45_05490"
     CDS             2509..3876
                     /gene="merA"
                     /locus_tag="VKO45_05490"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HMA05363.1"
                     /translation="MPPAYDLIIIGTGAAGTSAATRAHRLGAERIAMVERGPLWGTCV
                     NTGCIPSKLLLSIGDLAYYRNFGHAGLGVVTRLDPKEVIREKRDLILGLRDRKAHRLF
                     GEIGAELVEGTARFLSPREVEVGGRRLEARRFVIATGSSPSLPAVEGASSVPSLTSTG
                     ALDLAEVPESLIVVGGRSLGLEFAQIFAHFGSRVTLLQRSPRIIPEEEPEISSVMEDA
                     LKGEGIDIRTGAELLRVRKEGEGIGVSARVKGEAEKFSSARILFATGRTPNTRELDLA
                     ACGVKVDARGAIIVDDTMQTSVPHIWAAGDVTGEPMLEPWAGVGGAIAAENAMTGKGR
                     RIDRASLPHAIFTTPQVASVGLTEAQARAKGIAVKCRSVSLEGISRAVMTHDTRGLVK
                     MVAGEGSGKVLGVHVCAPLAAEIIEEGVLAVKFGLTTGDLVETFHVFPTMAGAIQDCA
                     RAFRS"
     gene            3932..4240
                     /locus_tag="VKO45_05495"
     CDS             3932..4240
                     /locus_tag="VKO45_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05364.1"
                     /translation="MATGQLAGFGISVLMIVIGVALCLYSVYDRMKNTEERAKRIAEE
                     VARRLEKRAGITTSPIPLGELDEIIREIMKIQNPSMQVGVFLTVFGILVMIISIFIPF
                     "
     gene            complement(4460..5245)
                     /locus_tag="VKO45_05500"
     CDS             complement(4460..5245)
                     /locus_tag="VKO45_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05365.1"
                     /translation="MTTIAEQYMNEMNKQFSCFATWEPGTPLKIGDIGELHHNEFTYV
                     SSLKEKGMDFNVRVDDSAGDLKYASGRSVKISTKASGQVALPQFNLDVGDAGIVVQFS
                     AEKAVVFEASGVEHRMITQVDQLDRYIEREKEADRWQDEWVIISDLMVAKSATVLISQ
                     GRDANIALRAKANVPNLSLSNVEAQFETAVKNQMNTTIVCQAGLTPLFKVRGIVRKFG
                     GLGGTKAGAIRDGPVFIKKEVHASAAHRISNAGEIPFSPFRLR"
     gene            complement(5387..5704)
                     /locus_tag="VKO45_05505"
     CDS             complement(5387..5704)
                     /locus_tag="VKO45_05505"
                     /inference="COORDINATES: protein motif:HMM:NF017917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HMA05366.1"
                     /translation="MAPSKNCHPELAINTTSFNGELVTGMPFPYKATKDLPEAVRNAL
                     PDHAQDIFLKAANAAWEKYRDPKDRRDPKESRDEVAYKIAWAAVERVYEKDPVTKTWR
                     KRP"
     gene            5770..6567
                     /locus_tag="VKO45_05510"
     CDS             5770..6567
                     /locus_tag="VKO45_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618527.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HMA05367.1"
                     /translation="MKTILYYFTGTGNSLAVAEGLARRLGDTEVVPIASLAGLPAPIT
                     PAAERVGIITPVYFFGLPSLVAVFSARLDLVKVNYVFAVATMGGSGGSAALRQLDSIL
                     KRGPGGKGLDAGFTVRMPGNYVLMYEPPSGKKQKELLKEADRQVEEIAEAVKKGRKVR
                     LPWSFFAFVVNGLMYPRFIAGVGEADRKFTVDDRCTNCGICEAVCPVENIRLEAGRPV
                     WLHHCEQCLACIQHCPTGAIQAGPETENRGRYRHPEVGIEDLKRRKV"
BASE COUNT         1281 a         2153 c         2111 g         1284 t
ORIGIN      
        1 ccatgacgat cacctcggcg aggtgatccg gctcgggaga aaaacggtcg ccatctcgga
       61 gatcgggaag ttcctcaggt caaagggtct acccgtagag acgatgaaca tcggcgggac
      121 catcacggtg gacggggtgg agttcaccat gacgcaggcc atccactcct ccgggctcga
      181 ggccgggggc ccggggtttt acggcggctg tccctgcggg ttcgtcatcg ggatggacgg
      241 ggtgcgggtt tatcacgccg gcgacaccgc actcttctcg gacatgaagc tcatcgggga
      301 gctctacgca cccgacatcg ccctcctccc tatagggggg aagttcacca tgggaccccg
      361 ggaggcgatg atggcggcga actatgtggg ggcaaagacg gtcatcccga tgcactacag
      421 cacctggccg gccatcctcc aggatcccct ctccttcaag cacgccatcg agcggaccac
      481 cgacctctcg gtcgtcatcc tctcgccggg ggaggcctac gaggtgccgg cccggtgagg
      541 agatagatgg ggcttctctc ctggtacgga attaagcatt gccggacgct cctggggaat
      601 tcgggcttcg ccctcacccc cgccgctgcg gcgtcccccg cgtgaggata gagcctatca
      661 ccgtataacc gtcggatcag gagatcccgg atccgaaggg ggatggccgc gccggggggc
      721 ggtagccccc caagagcact acgacgacga gaatgtacct aagaaccctt cctcttcctc
      781 atccggagaa acccgatcac gagtcctcac ccgaaccagt tcttctccgc gggaggcggt
      841 ccccggggaa tgacccacct gataccgcca tgatggccct tcgcgtcgcc cttataccgg
      901 gggatgacgt ggaaatggac gtgcatcacc gcctggcccg cgtatctccc gacgttcaca
      961 ccgatgttgt acccgtccgg gtggtgccgt tcctccacga cgccccttac cctgatgacg
     1021 aggtcgagga tggcggagcg ctcctccggg gtcgtatcga aaaaggacac gacatgccgg
     1081 aaggggatga cgagcatgtg cccccgggac acggggaagc ggtcggtcct cgcgtacgcg
     1141 agttcgttcc tgaggaggac ctccttttcg tcggggttgc agaaagggca gccgggctcg
     1201 gtctcgggaa tcgccatcac cggtatccac actcagggct cgaacccaga aaaaggttga
     1261 gatcgggtcg ccctgagccg ggccggatca gagctgcttt cccttcttgg ccctgcggct
     1321 gcagatgtgg gagtcgtact tcagcttcgc gttgaaggtc tcgaactcgg ccccgcagac
     1381 cgggcaggtc ttccggaaaa cccggtgata ggactgcttc tgtctctcag agagcatata
     1441 tcgcgttccc tctccttccg caatatggcg gccggaagat gatgaaggtg tgggtgagcg
     1501 gtgctccccg caggttttta tagggggatg agcggagttt atgccggtga tgccatggca
     1561 ggaccgaccc agtacagcgc ggtgaacctc ttcggcgagg cgcgggtcga ccccgcgagg
     1621 tggaaccgca ccccctccga ggaggaggtg gccggggccg tgaccgccat cgggaagagg
     1681 gggatccggg tgatccgcgc cagggacggg cgggaggccc tggacgctct cgtggggctc
     1741 atcccccggg gttcggaggt catgaacggc tcctccacga cgctcatcga gatcgggtac
     1801 gaaagcctcc tccggtcggg aaagacggga tggatcgatc tccatgaccg gatcaccgcc
     1861 gagaacgacg accgcaagag ggcggagctg cgccggaggt cggtggccgc cgaatacttc
     1921 gtctcggggg cgaatgccat cactcagacg ggggagatcc tcgcctgcga tgcgagcggg
     1981 agccgggtcg gcgcctggcc ctttgccgcc gggcacctga tcctcgttgc cgggatcaac
     2041 aagatcgtcc ccaccctcga ggacgccctc cgccgggtcc gggagtatgc cttccccctt
     2101 gagaacgccc gggccctccg ggtatacggg accccgagca tgatcgggaa gtgcgtcatc
     2161 ctcgcgaacg agaaggccga ggggagggtc accctgatcc tcgtcggcga atccctgggg
     2221 tactgatcat gcccgaaaac cccctcgaac acctcaaggc ccttatcggg aggtacacgg
     2281 tcatcacctc catccccgac atcgaccggt accggaggat caccgccccg aaggaggggc
     2341 actgcatctt ccgcggctgc cccgaaccca tcgcctggga ggatgcccag ggagggaatt
     2401 tcctctgcga gggtcactac cggaccatga agacctggat cgaggaggcg aggaagggcc
     2461 tcctgcctgg cgggcagctg agccctctct tcacccaggg aggaaccgat gccacccgct
     2521 tatgacctga tcatcatcgg cacgggcgcc gcaggcacat ccgccgcgac ccgggcgcac
     2581 cgcctcggtg cagaacgcat cgcgatggtg gagagaggcc ccctctgggg gacctgcgtg
     2641 aacaccgggt gcatcccgag caagctcctc ctctccatcg gggacctcgc ctactaccgg
     2701 aatttcgggc acgccggcct cggggtcgtg acccggctcg atccgaagga ggtgatccgg
     2761 gagaagcggg acctgatcct gggcctgcgg gaccggaagg cccaccggct cttcggggag
     2821 atcggggcgg agctcgtgga gggcaccgcg aggttccttt ccccccgcga ggtggaggtg
     2881 ggggggcgca ggctggaggc ccgccggttt gtcatcgcca ccggttcctc gccctccctg
     2941 ccggccgtcg agggagcctc ctcggtcccc tccctcacga gcaccggggc cctggacctc
     3001 gcggaggtcc ccgaatccct tattgtcgtc gggggacggt ccctcggcct cgagttcgcc
     3061 cagatcttcg cccacttcgg atcgagggtc accctcctcc agaggagccc gcggatcatc
     3121 cccgaggagg agccggagat ctcctcggtc atggaggacg ccctgaaggg cgaggggatc
     3181 gatatccgca ccggtgcgga gctcctgcgg gtgcgaaagg agggcgaggg gatcggcgtc
     3241 agcgcccggg taaagggaga ggcggagaaa ttctcctccg cccgcatcct ctttgccacc
     3301 ggccggactc ccaacacccg ggaactcgat ctcgcggcct gcggcgtgaa ggttgacgcg
     3361 aggggtgcca ttatcgtcga cgataccatg cagacctccg tcccccacat ctgggccgcg
     3421 ggggacgtga ccggcgagcc gatgctcgag ccctgggccg gggtcggggg ggccatcgcg
     3481 gcggagaacg cgatgaccgg caaggggagg aggatcgatc gtgcctccct cccccacgcc
     3541 atcttcacca ccccgcaggt ggcgagcgtc gggcttaccg aggcgcaggc ccgggcaaag
     3601 gggatcgccg tgaaatgccg gtcggtctcc ctcgagggga tctcgcgggc cgtgatgaca
     3661 cacgacaccc gcggcctcgt gaagatggtt gccggggagg gaagcggaaa ggtcctcggg
     3721 gtgcacgtct gcgcacccct cgccgccgag atcatcgagg agggggtcct cgccgtgaag
     3781 ttcgggctta cgacgggcga tctcgtcgaa accttccacg tgttcccgac catggccggg
     3841 gcgatccagg actgtgcgag ggcattccgg agctgatccg agagaatcaa atagcggccc
     3901 cttcataggg ttgaaacggg gtgtcgtgac aatggccaca ggtcagctcg ccgggttcgg
     3961 gatctcggtc ctcatgatcg tgatcggggt cgccctctgc ctctacagcg tgtacgaccg
     4021 gatgaaaaac accgaggagc gggcgaaaag gattgccgag gaggttgcga ggaggctcga
     4081 aaagagggcg gggatcacca cctcgccgat acccctcggg gagctggacg agatcatcag
     4141 ggagatcatg aagatccaga acccgtcgat gcaggtcggg gtcttcctca cggtcttcgg
     4201 gatcctcgtg atgatcatct ccatcttcat cccgttctaa agaattctcg cctccttctt
     4261 ttaccccctg cagggaagaa cctgattccc gtggcgaagt gtccatcgaa gaccggaaac
     4321 tcttccttca atggagggtt tcaacgccat tcagtcctcg tcgcgtggat ctgacgatac
     4381 aggaatgcca gcacctccgg ccctgtctca tgtcctccta tgtagccttc gaagagtccg
     4441 gttcttcagg gaacgggggc tatcgtaacc tgaaaggtga gaacggaatc tcccccgcgt
     4501 ttgatatccg gtgtgcggcc gaagcgtgta cctctttctt gataaataca gggccgtccc
     4561 ggatcgctcc tgcctttgtt ccccccagtc ccccgaattt cctgacaatc cccctgacct
     4621 tgaagagcgg ggtaagcccg gcctggcaga cgatggtggt gttcatctga ttcttcaccg
     4681 ccgtctcaaa ctgcgcctcg acatttgaaa gggataaatt cgggacgttc gcctttgctc
     4741 tcagggcgat gttcgcgtcc cttccctggg agatcaggac cgttgcagat ttcgcgacca
     4801 tcagatcgct gatgataacc cattcatcct gccatcgatc tgcctccttc tccctctcga
     4861 tgtaccgatc gagctgatcc acctgggtga tcatccggtg ttcaactccg gatgcctcga
     4921 aaacgacggc cttttccgcc gagaactgca ccacgatccc cgcatccccc acgtcgaggt
     4981 tgaactgggg gagagccacc tgtccgctcg ccttggtgga aatctttacc gaacggccgg
     5041 atgcatactt taaatcgccc gcggaatcat ccacccggac attgaaatcc atccccttct
     5101 ccttcaggct ggacacgtag gtgaattcat tgtggtgcag ctcgccgata tccccgatct
     5161 tcagcggagt ccccggttcc caggtggcaa aacaggaaaa ctgcttgttc atttcattca
     5221 tgtactgttc agcgatggtt gtcacaggat ctcacaagga gcaatccctg aggtaactat
     5281 atagtttttc gacgtgatgt aatcccgcgg gatgtcgggg cttcccttac cctccgcacc
     5341 aggggatcag gttttccccc ggatagaatc ctccggaaga tccctttcac ggcctctttc
     5401 tccaggtctt cgtcaccggg tccttctcat acactcgttc gacagccgcc caggcgatct
     5461 tgtaggcaac ttcatcgcgg gattcttttg gatcccggcg gtccttcgga tccctgtact
     5521 tctcccatgc cgcattcgcc gccttcagga agatatcctg ggcatggtca ggcagggcgt
     5581 ttctcaccgc ctcgggcagg tccttggtgg ccttgtaggg gaagggcatt cctgtcacca
     5641 gctccccgtt gaaggaggtc gtattaatag ctaattccgg gtgacaattt ttggagggtg
     5701 ccatcccatc cccggatggg atcgccaccc ttttccccga tgcctcacaa acgccatcca
     5761 aggttctcca tgaagaccat cctctactat ttcacaggca cgggcaactc cctcgccgtc
     5821 gcagagggcc tcgcccgaag gctcggggac accgaggtcg tgcccatcgc gtccctggcc
     5881 gggcttcccg ctcccatcac cccggccgcg gaacgggtcg ggatcatcac tcccgtgtac
     5941 ttcttcgggc tcccatccct cgtcgcagtc ttttcagccc ggctcgacct cgtcaaggtg
     6001 aattacgtct ttgcggtggc caccatgggc ggctcgggag ggtccgcggc gctgcggcag
     6061 ctggacagca tcctgaagag gggcccgggc gggaaaggtc tcgacgccgg tttcaccgtg
     6121 aggatgcccg ggaattacgt cctcatgtac gagcccccgt ccgggaaaaa gcagaaggag
     6181 ctcctgaagg aggccgaccg tcaagtggag gagatcgcag aggcggtgaa aaagggtcgg
     6241 aaggtcaggc ttccctggtc cttctttgcc ttcgtcgtca acggcctcat gtatccccgg
     6301 ttcatcgccg gggtgggcga ggctgaccgg aagttcaccg tcgacgaccg gtgtaccaac
     6361 tgcgggatct gcgaggcggt ctgccccgtg gagaacatcc ggctggaggc cggccgcccc
     6421 gtctggctcc accactgcga gcagtgcctg gcctgcatcc agcactgccc gaccggtgcg
     6481 atccaggcag ggccggaaac cgaaaaccgg gggcgatacc ggcacccgga ggtcgggatc
     6541 gaggatctca agaggcggaa agtctaaaaa gtaataaatt ttcagcgaac taagactgca
     6601 acccctcaac aagattccgt tacaaggggt tcgttaacct ccgtctttgt cggtgacaaa
     6661 aacatttttc tcccgacaga cgatgcttac tatcatgcca aagttctgtt ctgaatgcgg
     6721 aacgaaactt accaatgaaa atatgaaatt ctgccctgaa tgtggcaaac caatactgac
     6781 atcacccggt gcagctcagc aagaaaatca gtcacagagt gtatctcca
//
LOCUS       DATGDI010000146         4118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_218474, whole genome shotgun sequence.
ACCESSION   DATGDI010000146 DATGDI010000000
VERSION     DATGDI010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218474"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VKO45_05515"
     CDS             <1..319
                     /locus_tag="VKO45_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HMA05368.1"
                     /translation="ELLTGAMVVGLPLAPYTRVRAMIIDYFRRKFGEEGEFLSYTLPA
                     MNRALQALGRVIRSPEERGFLVLCEQRFLEPQIRGALPAWIREEMVETTREGFGQLVK
                     EWR"
     gene            310..768
                     /locus_tag="VKO45_05520"
     CDS             310..768
                     /locus_tag="VKO45_05520"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HMA05369.1"
                     /translation="MAVITGSEPFGLWVVTVSWQEDQVYRVHFGRTGPAGPVHPLLRR
                     YLRGKEVDLADLRSPAEEGDGMSAAVSRAVRRVGYGEVATYGEIARRVGTSPRAVGQV
                     MARNPTPLVIPCHRIVSKNGLGGFTPPLEIKRDLLAMERSARLGRSGTRR"
     gene            765..1352
                     /locus_tag="VKO45_05525"
     CDS             765..1352
                     /locus_tag="VKO45_05525"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HMA05370.1"
                     /translation="MRSAIVLVGGEARRAGGREKYFFSYEGRTFIEHILGALGSVVDE
                     IVLAARDPAQCERFSHLAGVRCVSDVRRGIGPTGGLHAGVLEARGDLVLVVACDMPCI
                     NARVIEHLFSLMDDYDAVIPAWNPEMLEPLHAVYRRSALLAYLQEPGHRSLREMVKAM
                     RVRFVKIEELRQMDPDLKTFTNINKIEELEKIKGG"
     gene            complement(1363..1500)
                     /locus_tag="VKO45_05530"
     CDS             complement(1363..1500)
                     /locus_tag="VKO45_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05371.1"
                     /translation="MARKETDPAAITRDVVEALELPDSALPILSRTVARHLRAMEREE
                     G"
     gene            complement(1586..2158)
                     /locus_tag="VKO45_05535"
     CDS             complement(1586..2158)
                     /locus_tag="VKO45_05535"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HMA05372.1"
                     /translation="MKRSLGARTLLYTHPVVVIGTYDKDGKPNAMTAAWAGICCSSPP
                     CVAVSLQRPRHTFRNIGETRAFTVNIPSEKYVRETDFLGMVSGKDTDKFAAAGLTPVK
                     SAKVNAPCVDEFPVVLECRLLQSFELGLHTQFVGEILDVRVDESVLGPDGMPEMGKVR
                     PFIYDSARQEYHAIGRLIGKAFSAGKELKG"
     gene            complement(2205..3041)
                     /locus_tag="VKO45_05540"
     CDS             complement(2205..3041)
                     /locus_tag="VKO45_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HMA05373.1"
                     /translation="MVWEDIMVKIAVTGKGGVGKTTIAAGLARIFGARGHRVVALDMD
                     PSPNLASSLSCGPEAANLLPLVEMEDLVLERTGAPPEGAGTVFRINPKVDDIPERFGI
                     PCRDGVRLLVLGSIRTGGGGCFCPANALARRLVDHLTGSSDILIMDMEAGVEHLGRGT
                     TRTVEALLVVTEPTVKSVEAAGQIVRLARDLGITRIYGVINKVRDREGEPFLSRLSAL
                     GVTPIGILPFDTAVQEAEEKGVPVFDLPGGEGIQAQIEGIATALEERLGPFGNQAGKK
                     GP"
     gene            3106..>4118
                     /locus_tag="VKO45_05545"
     CDS             3106..>4118
                     /locus_tag="VKO45_05545"
                     /inference="COORDINATES: protein motif:HMM:NF036965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HMA05374.1"
                     /translation="MGELIILPGGRRRPLEAGKTILSHLQELAVPVTAHCGGRGECRA
                     CAVDVDGSGLLSPRTAIEDSMLADPLQRLACQAVVERTDGILTVELPRYPRYSIVERG
                     LRPAVPFSPLVGPSADGGVPAVTRNGKVIGPYDGEIYGVALDVGTTTLAIRWLDLEDG
                     ENPDRYSASVLNPQIRFGDNVIDRIRYATGTPGGARHLQAAVVGAVNNLLACGAVRPD
                     HIYEMTVSGNSVMRDLFLGRPVERLGVFPFEPLTTSAVNTGASGLGIAIHPGGNVYAL
                     PLIGHFVGADTLALILATGMHEREEVNMAIDIGTNTEIALGNRDRILVTSCASGPAFE
                     GSG"
BASE COUNT          700 a         1334 c         1389 g          695 t
ORIGIN      
        1 agagctcctg acgggtgcaa tggtggtcgg gctacccctc gcgccgtaca cccgggtacg
       61 ggccatgatc atcgactact tccggaggaa gttcggggag gagggagagt ttctctccta
      121 caccctcccg gccatgaacc gcgcgctcca ggccctggga agagtcatca ggtccccgga
      181 ggagagagga tttcttgtcc tttgcgagca gagattcctg gagccacaga tccggggggc
      241 tcttcccgcc tggatcaggg aggaaatggt ggagacgacc cgggaaggat tcgggcagct
      301 ggtgaaggaa tggcggtaat cacgggctcc gagcccttcg gtctctgggt ggtgaccgtg
      361 agctggcagg aagatcaggt ctaccgggtc cacttcggga ggacggggcc cgcgggaccg
      421 gttcatcccc tcctccgccg gtacctccgg gggaaggagg tggatctcgc ggacctccgg
      481 tcacccgcgg aggagggcga cgggatgagc gcggctgtct cccgggcagt gaggagggtc
      541 ggctacggcg aggtggcgac ctacggggag atcgcccgga gggtggggac ctcgccccgg
      601 gccgtcggcc aggtcatggc ccggaatccc acccccctcg ttatcccctg ccaccggatc
      661 gtttcgaaga acggtctggg gggcttcacg ccgcctctcg agatcaagcg ggacctgctc
      721 gccatggagc gctcagcccg tttgggacgg agcgggacac ggcgatgaga tcggccatcg
      781 tcctcgtcgg gggagaggcg agacgggccg ggggccggga gaagtacttc ttctcctacg
      841 aggggaggac cttcatcgag cacatcctcg gggccctcgg gagcgtggtg gacgagatcg
      901 tccttgccgc gcgggacccg gcccagtgcg aacggttcag ccacctcgcc ggggtgaggt
      961 gcgtctccga tgtccgccgg gggatcggcc ccaccggggg cctccacgcg ggggtcctcg
     1021 aggcgcgggg ggatctcgtc ctcgtggtcg cctgcgacat gccctgcatc aatgcccggg
     1081 tcatcgagca cctcttctcc ctcatggacg actacgacgc cgtcatcccc gcgtggaacc
     1141 cggagatgct cgaacctctc cacgccgtct accggcgatc ggccctcctc gcgtacctcc
     1201 aggaaccggg ccaccggtcc ctccgggaga tggtgaaggc gatgcgggtg aggttcgtga
     1261 agatcgagga actgaggcag atggacccgg atctgaagac cttcacgaac atcaacaaga
     1321 tcgaggagct ggagaagata aaggggggat aagagcgggc aatcagccct cttcccgttc
     1381 catggctctc aggtgtcgcg ccaccgtcct cgagaggatc ggcagggccg agtcagggag
     1441 ctcgagggcc tcgacgacat cccgggtgat cgcggccgga tcggtctcct tccgggccac
     1501 ctccccctgc agggatctcg aaaaggatct tcagcgagtg aacggattcc gcgaccttca
     1561 tcgcgcggtc cccggcggtc agggattacc ccttcagctc cttccctgcg gagaaggcct
     1621 tcccgatgag tcttccgatg gcatggtact cctgcctcgc cgaatcgtag ataaagggac
     1681 ggaccttccc catctccggc atcccgtcgg ggccgaggac cgattcatcc accctcacgt
     1741 cgaggatctc acccacgaac tgcgtgtgga ggccaagctc gaaggactgc aacaggcggc
     1801 actcgaggac cacggggaac tcatccacgc agggagcgtt caccttcgcg gacttcaccg
     1861 gagtgagacc tgcggcggcg aacttgtcgg tgtccttccc cgagaccatc ccgaggaaat
     1921 cagtctccct gacgtacttc tcggagggga tgttgaccgt gaaggccctg gtctccccga
     1981 tgttcctgaa ggtatggcgc ggcctctgga gggagaccgc gacgcagggc gggcttgaac
     2041 agcagatccc cgcccaggcg gcggtcatgg cattgggttt cccgtccttg tcataggtcc
     2101 cgatgacaac aaccgggtgg gtgtagagaa gcgtgcgggc cccgagagat cgtttcatgc
     2161 ctgacccttg ggtatcccaa ggcgatgaag gtgtgggtac atattcaggg ccccttcttc
     2221 ccggcctggt tcccaaaggg cccgaggcgc tcctcgaggg ccgtggcgat cccttcgatc
     2281 tgagcctgga tcccctcccc tccggggagg tcgaagacgg gcaccccctt ttcctccgcc
     2341 tcctggacgg cggtgtcaaa ggggaggatc ccgatgggtg taaccccgag ggccgagagc
     2401 cgggagagga agggctcccc ctctctgtcc cggaccttgt tgatcacacc atagatccgg
     2461 gtgatcccga ggtcccgggc cagtcgcaca atctgtccgg cggcttccac cgacttcacc
     2521 gtcggttcgg tgaccacgag gagtgcctcc acagtccggg tcgtcccccg ccccaggtgc
     2581 tccaccccgg cctccatgtc catgatgagg atatcggagc tgcccgtcag gtggtcgacg
     2641 agcctccggg cgagggcgtt cgccgggcag aagcagccgc cgccgcccgt gcggatggag
     2701 ccgaggacga ggagacgtac cccatccctg caggggatcc cgaaccgctc cgggatatca
     2761 tccaccttcg ggttgatccg gaagaccgtg ccggcaccct cgggaggagc cccggtcctc
     2821 tccaggacca ggtcctccat ctccacgagg gggaggaggt tcgcggcctc cgggccgcag
     2881 gagagggagg aggcgaggtt cggcgagggg tccatgtcga gggccacgac ccggtgcccc
     2941 cgggccccga agatccgggc gagccccgcg gcgatggtag tcttcccgac acctcccttg
     3001 ccggtgaccg ctatcttgac catgatgtcc tcccatacca tgatgagcct cggatataaa
     3061 ttcaaggtat ccacgggggt ctgcacccgg ggagagggag gacggatggg ggagttgatc
     3121 atccttcccg gggggaggag gaggccgctc gaggcaggga agaccatcct ctcccacctc
     3181 caggaactgg cggtgccggt gaccgcccac tgcgggggca ggggagagtg ccgggcctgc
     3241 gcagtagacg tcgacgggag cgggttgctc tccccgagga cggccatcga ggactcaatg
     3301 ctcgcggacc ctctccagcg gctcgcctgt caggcggttg tggagaggac ggacgggatc
     3361 cttacggtgg agcttccccg ctatccccgg tactccatcg tcgaaagggg ccttcgcccg
     3421 gccgtgccct tttctcccct cgtggggccc tcggcagacg gtggtgtccc ggcggtcacc
     3481 cggaacggga aggtcatcgg tccctacgat ggggagatct atggcgtcgc ccttgacgtg
     3541 gggaccacca cgctcgccat ccgctggctg gacctggagg acggggagaa ccccgaccgg
     3601 tactccgcat cggtcctgaa cccccagatc cggttcgggg acaatgtcat cgaccgcatc
     3661 cggtatgcca cgggaacccc cggaggggca cggcacctcc aggccgcagt cgtcggggca
     3721 gtcaacaacc tcctcgcctg cggtgcggta cgaccggacc atatctacga gatgaccgtt
     3781 tcagggaaca gcgtgatgcg ggatctcttc ctcggccggc cggtcgagag gctgggggtc
     3841 tttcccttcg aacccctgac cacatccgcg gtgaataccg gcgcgtcggg actcgggata
     3901 gccatccatc ccggggggaa tgtctatgcc cttcccctca tcggccactt cgtgggggcc
     3961 gataccctcg ccctcatcct cgccaccggg atgcacgaga gggaagaggt gaatatggcg
     4021 atcgatatcg gaacgaacac cgagatcgct ctcgggaacc gggaccggat cctcgtcacc
     4081 tcctgtgcct ccggcccggc cttcgagggc tcggggat
//
LOCUS       DATGDI010000147         4412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_422137, whole genome shotgun sequence.
ACCESSION   DATGDI010000147 DATGDI010000000
VERSION     DATGDI010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422137"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VKO45_05550"
     CDS             complement(<1..544)
                     /locus_tag="VKO45_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMA05375.1"
                     /translation="MDRRTSVLIVSVILAAGIIGIMLFTVRDDLLLAMGHWNPLYLVP
                     AIVFSTAGWWLRGARYRSILGKLGILAGLAFSTACVLISQTANLIIPARLGDLVRVFI
                     LKHENLTGYATGISSIVIERFFDILTVAVLGLLALPFVPGIPPWVYWVIVVPLALCGV
                     FVVLLLALGKLHAEHRILAIL"
     gene            complement(534..1244)
                     /locus_tag="VKO45_05555"
     CDS             complement(534..1244)
                     /locus_tag="VKO45_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HMA05376.1"
                     /translation="MGEIEVSMVIPVFNDRAALETAIPRSLEALGQVTSAFELIIAED
                     GSTDGTPGFVREVAASDPRVRLLHSDERLGRGRALNRAFGEARGKIIGYYDADLATDL
                     RHLPDLIGAIRAGADIATGSRLLPSSDVKRTGGRELASRVYNALVRLILGSRLHDHQC
                     GFKAFNRDRILALIPSVRAPHWFWDTEVLVRGQRAGYRVAEFAVSWRTGRTTTVRLKD
                     VFEMGGAILSLWWQLHGP"
     gene            complement(1237..2472)
                     /locus_tag="VKO45_05560"
     CDS             complement(1237..2472)
                     /locus_tag="VKO45_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMA05377.1"
                     /translation="MRICILGGGLTGLSAALRLSSSFEVTLLERDMEPGGCLSSYRLD
                     GGWVERFYHHCFAGDVHLLALLGEIGLSDRLEWLRGTTGSFAGGKVFPLNTPLEILKY
                     PYLGTFDKARLALFTLGARRLKAEDLDGVTAKDFVTRRLGEGIYREFFRPLLRSKFGD
                     REGEVSAGWLVSRVAIRSNRGLSGERLGYLRGGFHLLVERLAELARKRGCTVEMGTPV
                     TRIERHLGGWSVNGKPYDRVLSTIPPQEVTRLGGPDLPAIPYQGAACMTLGLDRDVTG
                     GVYWLNMNDPAPYGAVVSHTNFIPPERYGGERILYLASYFQGTMDPALPGRMVEHFCG
                     RFGVPPEAIRWHRMAVEPFAGPVYTTGYRGLMPAYEEKGLFLAGMFSRPNYPERSMEG
                     SIVAGTEVAGKIGGAVAHG"
     gene            2594..3784
                     /locus_tag="VKO45_05565"
     CDS             2594..3784
                     /locus_tag="VKO45_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin/FtsZ family protein"
                     /protein_id="HMA05378.1"
                     /translation="MRVFFIGFGQAGGKIADMFIEQDKRMAAQNFRAISVNTARTDLM
                     GLTNIGLRDRILIGQTVVKGHGVGTDNVTGAKVTSDEIDSIINAIDSRGTHDIDAFVI
                     IAGLGGGTGSGGSPVLARALKRIYREPVYALGILPAPEEGRLYSYNAARSLSTLVNEV
                     DNTFLFDNSAWKNEGESIKSAYQRLNDEIVRRFGVLFRAGEVTKMGVGEMVVDSSEII
                     NTLRGGGISSVGYAITEVISRRTKQKKGIFGGLKDSIGKATSGKKEPTEEVLLGEDKS
                     AKIIALVRRAMLGRLTLPCDYSSAERALVLIAGPPEEMDRKGVEKSKSWVEENIAGIE
                     VRGGDYPVDSNFIAAVVVLATVGNAPRIRELLEIAKETKEDAMKVKEKKVSLFDEDIE
                     PLFE"
     gene            3793..>4412
                     /locus_tag="VKO45_05570"
     CDS             3793..>4412
                     /locus_tag="VKO45_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05379.1"
                     /translation="MKNFSAILLCFIVALCLVQVTAALSASGITTSPTQVNALKPGDV
                     ISEVSGTINLPSSGDMTFNTDDAVEFYTQLDSARWSVSIVIGGIENPARTYGGKHVTI
                     GGYDLAYPTRGYSSVALKFSMMGGTVPSSFTSGPITLVRVVETDPDGDQVGNAVYVNG
                     TVISTDVMRANITRLNGDLTNLKASIDEKENTGVDLTVPLQKYQTA"
BASE COUNT          869 a         1364 c         1475 g          704 t
ORIGIN      
        1 ggaggatggc gaggatccgg tgctcggcat ggagcttccc gagggcgagg aggaggacga
       61 caaagacgcc gcagagggcg agggggacga cgatgaccca gtaaacccac ggggggatgc
      121 ccgggacgaa ggggagggcg aggaggccga ggacggccac ggtgaggata tcgaagaacc
      181 gctcgatgac gatggaggag atcccggtgg cataccccgt gaggttttcg tgcttcagga
      241 tgaagacccg gacgaggtcg ccgaggcgtg ccgggatgat gaggttcgcc gtctggctga
      301 tgaggacgca ggcggtggag aaggcgaggc cggcgaggat cccgagcttc ccgaggatgg
      361 agcggtaccg tgccccccgg agccaccacc cggccgtcga gaagacgatg gccgggacga
      421 ggtagagggg gttccagtgg cccatcgcga ggaggaggtc gtcccggacg gtgaagagca
      481 tgatgccgat gatcccggcc gcgaggatca ccgagacgat gaggacgctc gtcctacggt
      541 ccatggagct gccaccagag ggagaggatc gcccccccca tctcgaagac atccttcagg
      601 cgcaccgtcg tggtcctccc cgttctccag gaaacggcga actccgcgac ccggtaaccg
      661 gctcgctgac ctcgcacgag cacctcggta tcccagaacc agtggggggc cctcaccgag
      721 gggatgaggg cgaggatccg atccctgttg aaggccttga agccgcactg gtggtcatgg
      781 agcctgctcc cgagaatgag ccggacgaga gcgttgtaca cccggctcgc gagctcccgc
      841 cccccggtcc gcttcacgtc gctcgacggg aggagccggg acccggtggc gatgtcggca
      901 ccagcccgga tcgccccgat gagatcgggg aggtgccgga ggtcggtggc gaggtccgcg
      961 tcatagtacc cgatgatctt cccccgggcc tccccgaaag cccggttgag ggcccgcccc
     1021 ctgcccaggc gttcgtcgga gtggaggagc cggacccggg ggtctgatgc cgccacctcc
     1081 cggacaaaac cgggcgtccc atcggtggac ccgtcctcgg cgatgatgag ctcgaatgcg
     1141 gaagtaacct gcccgagggc ctcgagggat ctcgggatgg ccgtctcgag ggccgcccgg
     1201 tcgttgaaga ccgggatgac catggagacc tcgatctcac ccatgggcga ccgctccccc
     1261 gatcttcccc gcgacctcgg ttcccgcaac gatggagccc tccatggagc gttcggggta
     1321 attgggccgg gagaacatgc ccgcgaggaa gagccccttc tcctcgtagg ccggcatgag
     1381 cccccggtat cccgtggtat acaccggccc tgcgaagggc tcgacggcca tcctgtgcca
     1441 ccggatagcc tccggcggca cgccgaagcg gccgcagaaa tgctccacca tcctcccggg
     1501 gagggcggga tccatggtgc cctggaagta ggaggcgagg tacaggatcc tctccccgcc
     1561 atatcgctcg gggggaatga agttcgtgtg ggagaccacc gccccgtagg gggccgggtc
     1621 gttcatgttg agccagtaca cccccccggt cacgtcccgg tcgaggccga gggtcatgca
     1681 ggcggccccc tggtagggga tggcgggcag atcgggaccc ccgagcctcg tcacctcctg
     1741 gggggggatg gtggagagaa cccggtcata gggtttccca ttcactgacc atccgccaag
     1801 atgtctctcg atccgggtca ccggtgtccc catctcgacg gtacaccccc ttttcctggc
     1861 gagctccgcg aggcgttcca cgaggaggtg gaagcctccc cggaggtacc ccaggcgctc
     1921 cccggagagc ccccggtttg accggatggc gacccgggag acgagccatc ccgccgagac
     1981 ctcgccttcc cggtccccga acttgctcct gagaaggggc cggaagaact cccggtagat
     2041 cccctccccg agacgccggg tcacgaagtc cttcgccgtg accccgtcca gatcctcggc
     2101 cttcaacctg cgtgccccca gggtgaagag ggcgagcctc gccttgtcga aggtccccag
     2161 gtacgggtac ttcaggatct cgaggggggt gttcaggggg aagacctttc cccctgcaaa
     2221 ggaccccgtg gtgcctctga gccactcgag ccggtccgag aggccgatct ccccgagaag
     2281 ggcgaggaga tgcacatccc cggcgaagca atggtggtag aaccgctcca cccagccccc
     2341 gtcgagccgg taggaggaga gacaccctcc cggttccatg tccctctcga ggagggtcac
     2401 ctcgaaggag gatgaaagcc tgagggccgc cgagaggccg gtcaggcccc ccccaaggat
     2461 gcaaatcctc atcgtaagtc caatagttta tggggaacgg tgcccataag aaagtttttg
     2521 aactatctgg aagagaatta ggtaagggaa tagagctcac cgggatggga ttcctgctgg
     2581 ttggaggcac ttaatgcggg tatttttcat tggatttggc caggcgggcg gcaaaatcgc
     2641 agacatgttt atagagcagg acaaacgaat ggccgcccag aatttcaggg ccatttccgt
     2701 gaacaccgcg aggaccgacc tcatgggcct gacgaacatc ggcctccggg accggatcct
     2761 catcggccag accgtcgtga agggccatgg cgtgggtacc gacaacgtca ccggggcgaa
     2821 ggtcacctcc gacgagatcg acagcatcat caatgccatc gactcccggg ggacccatga
     2881 catcgacgcc tttgtcatca tcgccgggct tggcgggggt accggctccg gtggctcgcc
     2941 ggtcctcgcc cgggccctga agcggatcta ccgggagccg gtctacgccc tcgggatcct
     3001 cccggccccc gaggaggggc gcctctattc ctacaacgcc gcccggagcc tctcgaccct
     3061 cgtgaacgag gtggacaaca ccttcctctt cgataactcc gcctggaaga acgaggggga
     3121 gagcatcaag tcggcctacc agcggctgaa cgacgagatc gtccggaggt tcggtgtcct
     3181 cttccgggca ggcgaggtga cgaagatggg cgtcggcgag atggtggtcg actcctccga
     3241 gatcatcaac accctccggg gcggggggat ctcctcggtc gggtatgcca tcaccgaggt
     3301 gatatcccgg cggaccaagc agaaaaaggg catcttcggc ggcctgaagg attccatagg
     3361 aaaggctacg tcggggaaga aggagcccac cgaggaggtt ctcctcggcg aggacaagtc
     3421 cgcgaagatc atcgccctcg tgcggcgggc catgctcggg cgcctcaccc tcccctgcga
     3481 ctactcctcg gccgagcggg ccctcgtgct catcgcgggt ccccccgagg agatggatcg
     3541 gaaaggggtg gagaagtcca agagctgggt ggaggagaac atcgccggta tcgaggtccg
     3601 ggggggcgat tacccggttg actcgaactt catcgcggcg gtcgtggtcc tcgcgacggt
     3661 agggaacgcc ccgaggatcc gggagctcct ggagatcgcc aaggagacga aggaggacgc
     3721 catgaaggtc aaggagaaga aggtctccct gttcgacgag gacatcgagc ctctcttcga
     3781 gtgaggagga gtatgaagaa tttttcagcc atcctgctct gtttcattgt agctctctgc
     3841 ctggtacagg tgaccgcagc cctcagtgcc agcgggatta cgacgagtcc cacacaggtg
     3901 aacgccctga agcccgggga tgtcatctcc gaggtcagcg ggaccatcaa cctgccctcg
     3961 agcggggaca tgaccttcaa caccgatgac gctgtagaat tctacacgca gctggacagc
     4021 gcccgttggt cggtttccat cgtcatcggg gggattgaaa acccagcgag gacctacggg
     4081 gggaaacatg tcaccatcgg cgggtacgat ctggcatacc cgacaagagg ctactcgagc
     4141 gtggccctga agttctccat gatgggcgga acggtgcctt cctccttcac gagcgggccc
     4201 atcaccctcg tcagggttgt cgagaccgat cctgacggcg accaggtcgg gaacgcggtg
     4261 tacgtgaacg ggactgttat cagcacagat gtcatgaggg caaatattac cagactgaac
     4321 ggggatctca cgaacctgaa ggcgtccatc gatgaaaagg agaacaccgg ggttgacctg
     4381 actgtccccc tccagaaata ccagaccgcg aa
//
LOCUS       DATGDI010000148         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_44194, whole genome shotgun sequence.
ACCESSION   DATGDI010000148 DATGDI010000000
VERSION     DATGDI010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44194"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3147
                     /locus_tag="VKO45_05575"
     CDS             <1..3147
                     /locus_tag="VKO45_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05380.1"
                     /translation="AYPVELNVTSDHPWLVADGKDTAIITANVVYGTGPLAGQPIAGA
                     NVSFAVDYPWQVTKTFLLTNVNGTATTTFLDTRNSGTANITVTAWTMIETENWGYMNY
                     SDTKTVSQRIDHTTPASTLVSYQNKVQVGNSTRISVLVKDRWGNPVDNRNLVETMRFV
                     ASSSGPCGFESNGSWVKSITVPVNASGYADVQYLVYPTGANYIFIDMPSPISSRLISI
                     EGTAQSQPFSISTFVSPGGSPYPYTQIQSGKFTIGFTLYDRYGFPTMNQPVNITTNIP
                     GESMSLSTNKNGLVIITYGPKDIAGIYTITATAINNRSVSVSQKVEFVSGAAADALLT
                     ASPQTMASRDVTSVEYPEGIKSSLIMRVMDVKGNPVAGEDVTFRFTNFDVNNNLVNQT
                     IGPVLEDTSGVTSTSALNVDIHVVSDESGEAIVYFHPGAFTTDVTAKKYNASAEGKAT
                     VQAQWSGVKRQVTLKYINYRYLTIESRVDPTTVRVNESVDLIVTVRGDGYALQPKPID
                     VVLVTDRSGSMLEDNPDREVSIIGAASVFADQLEYSRDRLGQVTFGGNSWVDPRNQKN
                     SGSDSTTSDDWDYATLHYNLSTRYNDYATTDLALSSTKNLIKSKIASVTPTANTPMRY
                     GIFRAIYDNRNAWNPTSVRALILLTDGDYNIYGDPLARGSVGPTTATDTGTYYDATVT
                     KYWNISKANGLIDLPGPKTGPTSLQNMSNYARSYNVRIYTIGFNAGSLSGGALTALRN
                     LAKQTGGNFYEADATNIADVYTQIAGALRDEAGVNTTMNLSFGNVKVGNVTMPGKQVY
                     SYQRIPGRSTRVNTWNASVSPSFPGYPKEYNQWDEWNTTQRLDFFIGTIRLGQTWESK
                     VTFKVLQEGNINVFDALNSRVSVENNGTVRLNIPEVYITALPNLSGSSPMGAATLHVL
                     NLRLTNEGSNTSMDLAWNLSYNGLYPISEEIAISPCGTDYWNPLGTKQVSNLTTSDTG
                     SYAFDNLPIGNYMIRVQGNADDAPMDEDFLYLKYNGHSISKYTDPPETCSFGGAWPGW
                     TEGIFSKPPPTLPKIRIS"
     gene            3435..4112
                     /locus_tag="VKO45_05580"
     CDS             3435..4112
                     /locus_tag="VKO45_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HMA05381.1"
                     /translation="MKVLGINGSPRGKESQTLRLVVAALDGAEVEGAETELIDICDYD
                     LEFCTACGTCYAKGECIHPDDYEEIFGKILDADGIVLGSPNYIDSITAQLKVLFDRMA
                     DAIHCQMLSGKYGCSVCTAGGSNEMEVVGYMNRVLANLGAQIVGGVGVAFRGNPEAIL
                     PGEKKAYEMGRDLVKAIRTQRSYPDQEANLRTRRDYFCQLIQANRERWAHEYDWWVQA
                     GVIKEKE"
     gene            4169..5068
                     /locus_tag="VKO45_05585"
     CDS             4169..5068
                     /locus_tag="VKO45_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA05382.1"
                     /translation="MTEDTYPTATVQGKEVPWDPTTLRRIRDHPCFSEKACHAFGRMH
                     LPVAPKCNIQCNYCIRDFDCVNESRPGVTTRVLSPPEAIEMVKKALEKFTYIKVLGIA
                     GPGEPLYNEETFETLRLAKIEFPNLIKCISSNGLLLPEKIDLLEKYDVGNVTVTWNAL
                     NPSTGAKIYSWVTWEGRRLHGEEGAKLLLKNQLAGIEEAVKRHMIVKVNTVYIPGVND
                     MEIPDIAKKASELGVYTFNVIPLIPQYKFAGVTPPTPADKRKMQDECSKYIRQMRHCQ
                     RCRADAIGRLGHDVQGCLYSGSK"
BASE COUNT         1177 a         1625 c         1477 g          920 t
ORIGIN      
        1 gcataccccg tggagctcaa cgtgacgagc gatcacccgt ggctggtggc cgacggcaag
       61 gacacggcca tcatcacggc gaatgtcgtg tacggcacag gtcctctcgc cgggcagcct
      121 atcgcgggtg ccaatgtcag cttcgcggtc gattacccct ggcaggtgac gaaaaccttc
      181 ctcctcacca atgtgaatgg aaccgccacc acgaccttcc ttgacacaag gaacagcgga
      241 accgccaata tcacggtgac tgcgtggacc atgatcgaaa ccgagaattg ggggtacatg
      301 aactatagcg acacaaagac cgtcagccag cgcatcgacc ataccactcc tgcaagcacc
      361 ctggtgagtt atcagaacaa ggtgcaggtg gggaactcaa cacggatctc ggttctcgtc
      421 aaggacaggt gggggaaccc ggtggacaac cggaacctgg tcgagacgat gaggttcgtc
      481 gcaagctcct cgggcccctg cggatttgaa tccaatggat cctgggtaaa gtccatcacg
      541 gtcccggtga atgcctcggg atatgcggat gtacagtacc tggtgtaccc cacaggagcg
      601 aactatatct tcattgatat gccatccccc atctcctcca gactcatctc cattgaaggt
      661 accgctcaga gtcagccctt ctcgatatcc accttcgtat cccccggtgg ttcaccgtac
      721 ccctacaccc agatacagtc cgggaagttc accataggtt tcacgctgta cgacaggtac
      781 ggattcccca ccatgaacca gcccgtcaat atcaccacga acatccccgg cgagtccatg
      841 tccctttcca cgaacaagaa cgggctcgta atcatcacct acggtcccaa ggatattgcc
      901 ggtatctaca ccatcaccgc gacggccata aacaaccgct cggtctcggt ttcccagaag
      961 gtggagttcg tgagcggcgc agccgcagat gcactcctga cggcaagccc ccagaccatg
     1021 gccagccggg atgtgacctc ggtggagtac cccgagggca tcaagtcctc tctcatcatg
     1081 cgggtgatgg atgtgaaggg aaacccggtt gcaggggagg atgtcacctt ccggttcaca
     1141 aacttcgatg tcaataataa cctggtgaac cagacaattg gcccggtcct cgaggatacc
     1201 agtggagtga cgagcaccag cgccctgaac gtcgatatcc acgtagtttc ggacgagagc
     1261 ggtgaggcca tcgtctattt ccacccgggc gccttcacga ccgatgtgac cgccaagaag
     1321 tacaatgcat cggcggaagg gaaggccacg gttcaggccc agtggtctgg cgtgaagcgg
     1381 caggtgaccc tgaagtacat caactaccgg tacctcacca tcgagagccg ggtggacccg
     1441 acgaccgtca gggtcaacga atccgtagac ctcatcgtaa ccgtgagagg cgacgggtac
     1501 gccctgcagc cgaagcccat tgacgtggtc ctcgtcaccg accgctcggg atccatgctc
     1561 gaggataatc ccgaccggga ggtgagtatc atcggtgcgg catcggtttt cgccgaccag
     1621 ctcgagtatt cccgagatcg cctgggacag gtcaccttcg gcgggaattc gtgggtggac
     1681 cccaggaacc agaaaaacag cggcagtgat tcgaccacga gcgatgactg ggactatgcc
     1741 accctgcact acaacctgag cacccggtac aatgactatg ccacgaccga tctggcgctc
     1801 agctccacca agaacctcat caaatccaag atcgcatccg tcaccccgac cgcgaatacc
     1861 cccatgaggt acggcatttt cagggcgatc tacgacaacc gcaatgcctg gaatcccacc
     1921 tccgtgaggg ccctgatcct cctcaccgat ggggactaca acatttacgg cgatcccctc
     1981 gcccgtggat cggtaggtcc caccactgcc accgacaccg gcacctatta cgatgcaacg
     2041 gtcacgaagt actggaacat ctccaaggca aacggcctca tcgatctccc cggaccgaaa
     2101 acgggaccga cgtccctcca gaacatgtcc aactacgcga ggtcgtacaa cgtcaggatc
     2161 tacaccatcg ggtttaacgc aggtagtctc agcgggggag ccctaaccgc cctgagaaac
     2221 ctagcaaagc agacgggtgg taatttctac gaggcggatg cgaccaacat tgccgatgtc
     2281 tacacacaga tcgcgggggc cctccgggac gaggccgggg tgaacaccac catgaacctc
     2341 tccttcggaa atgtcaaggt ggggaatgtc acgatgcccg ggaagcaggt gtacagctac
     2401 cagcgcatcc ccggccgctc cacccgggta aacacctgga acgcgagcgt gagcccctcc
     2461 ttccccggct acccgaagga gtataaccaa tgggacgaat ggaacaccac ccagaggctt
     2521 gacttcttca tcgggaccat ccgcctcggc cagacctggg agtcgaaggt caccttcaag
     2581 gtcctccagg aggggaacat caatgtcttc gatgccctca actcgagggt cagcgtggag
     2641 aacaatggaa cggtcagatt gaacattccc gaagtgtaca tcacggcact cccgaacctc
     2701 tccggatcca gtcccatggg cgcggcgacc ctccatgtcc tgaatctgag gctgacgaac
     2761 gagggctcga acacgagcat ggacctggca tggaacctct cgtataacgg tctgtatccc
     2821 atctcagagg aaatcgcgat ctccccgtgc gggaccgatt actggaaccc cctgggtacg
     2881 aagcaggttt cgaatctgac cacgtccgat acggggagct atgccttcga caatctcccc
     2941 atcgggaact acatgatccg ggtccagggg aacgcggatg atgcccccat ggacgaggac
     3001 ttcctctacc tcaaatacaa cgggcacagt atatccaagt acacggatcc tccggaaaca
     3061 tgttcattcg gtggcgcctg gcccggctgg accgagggaa tcttcagcaa gcccccgccc
     3121 acgttaccga agatccggat aagctgacgg ggaggagatt ccccccccct tttttaggag
     3181 atcgaggatt ttttaaacgt actctctcga gaccaggtca aaccaggttc ccttctcgaa
     3241 cttctgggtc ccgtatctcc aacagggcga gagccccggg atcgtgtatc ggccatgcag
     3301 gggaaggcga ccggtgagct tctcccgcat tcaccatcga tccctgaccg gccgaccttc
     3361 cggggccctt cacgccgtgg attcccaatc cttaatacct cccctcccga ttcctctacc
     3421 ggagggtgat tcccatgaag gttcttggca tcaatggaag cccgaggggg aaggagagcc
     3481 agacgctgcg gctcgtcgtc gcggccctgg acggggccga ggtggagggg gcggagacgg
     3541 agctcatcga catctgcgac tacgacctcg agttctgcac cgcctgcggg acctgctacg
     3601 cgaaggggga gtgcatccac cccgatgatt acgaggagat cttcgggaag atcctcgatg
     3661 ccgacgggat cgtcctcggt tccccgaact acatcgactc catcacggcc cagctgaagg
     3721 tcctcttcga ccggatggcc gatgcgattc actgccagat gctctcgggg aagtacgggt
     3781 gctcggtctg caccgcgggg ggctcgaacg agatggaggt ggtggggtac atgaaccggg
     3841 tccttgcgaa cctcggcgcc cagatcgtcg gcggggtcgg cgtggccttc cggggaaacc
     3901 ccgaggcgat cctcccgggc gagaagaagg cctacgagat gggacgggac ctcgtgaagg
     3961 ctatccggac gcagcgctcc taccccgacc aggaggccaa cctccggacc cgcagggatt
     4021 acttctgcca gctcatccag gcgaaccgcg agcggtgggc ccacgagtac gactggtggg
     4081 tgcaggcagg agtcatcaag gagaaggagt aggcggtact ctccccccaa ttgatatagg
     4141 ataagtatca tcatgatctg gtagaaccat gaccgaggac acctacccga cggcaacggt
     4201 ccaggggaag gaggtcccct gggacccgac caccctccgc cggatccggg accatccctg
     4261 cttctcggag aaggcctgcc atgccttcgg ccggatgcac ctccccgtgg ccccgaagtg
     4321 caacatccag tgcaattact gcatccggga ctttgactgc gtgaacgagt cgaggcccgg
     4381 ggtcaccacc cgggtgctct ccccgccgga ggccatcgag atggtgaaga aggccctcga
     4441 gaagttcacc tacatcaagg tcctcgggat cgcgggcccg ggcgaacccc tgtacaacga
     4501 ggagaccttc gagacgctgc gcctcgcaaa gatcgagttc ccgaacctta tcaagtgcat
     4561 cagcagtaac ggcctcctcc tcccggagaa gatcgacctc ctggagaagt acgacgtcgg
     4621 gaacgtgacc gtcacctgga acgccctcaa cccctccacg ggcgcaaaga tctactcctg
     4681 ggtcacctgg gaggggaggc ggctccatgg cgaggaggga gcaaagctcc tcctcaagaa
     4741 ccagctcgcg ggcatcgagg aggcggtgaa acgccacatg atcgtgaagg tgaacaccgt
     4801 gtacatcccc ggggtgaacg acatggagat cccggacatc gcgaagaagg cctcggagct
     4861 cggggtctac accttcaatg tcatccccct catcccccag tacaagttcg ccggtgtgac
     4921 gcccccgacg cctgccgata agaggaagat gcaggacgag tgctcgaagt acatccggca
     4981 gatgcggcac tgccagcggt gccgggcgga cgccatcggc cggctcgggc acgatgtcca
     5041 gggctgcctg tactcaggga gtaaataacc cgtctccatt tttcccttcc ccccgaggag
     5101 atacctttag agatccaaat cctgggttta ttgagataat tcgtacttgt catgggctct
     5161 tgggggaggg agggttcatc agaacccgac ctgaagcaa
//
LOCUS       DATGDI010000149         1879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_44468, whole genome shotgun sequence.
ACCESSION   DATGDI010000149 DATGDI010000000
VERSION     DATGDI010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44468"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /gene="nrdD"
                     /locus_tag="VKO45_05590"
     CDS             complement(<1..606)
                     /gene="nrdD"
                     /locus_tag="VKO45_05590"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HMA05383.1"
                     /translation="MVEWDRERIVRQIVQETRLIEVFYGHESATEEIAREIAVLVEQR
                     ILKLGLQVLSGPLIREITNIVLLEKGLTEYRNVCTRVGTPVYDAHLIDVGRGFEAHDN
                     ANLQENAETSHKKKADKISKEQYLLQLPPHLSDYHLGGDLHIHDLEYFGTRPFCQDWD
                     LRYFLYYGLMPDGNGTKASVAGPAKRAEVAVLHAVKALGSAQ"
     gene            complement(927..1196)
                     /locus_tag="VKO45_05595"
     CDS             complement(927..1196)
                     /locus_tag="VKO45_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HMA05384.1"
                     /translation="MSEKPVLTVYTLENCPNCEMLKGYLHSLRVTFAEKDMSSAESLT
                     ELRVNGVFAMEAPVLRKDETFLTSADLFSGGKVRAAELNRTLDLK"
     gene            1253..1795
                     /gene="thpR"
                     /locus_tag="VKO45_05600"
     CDS             1253..1795
                     /gene="thpR"
                     /locus_tag="VKO45_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HMA05385.1"
                     /translation="MVRTFIAIDLTPEVRERMRGPQASLAGCRARLTLVKPPSIHITL
                     KFLGEIDEKTLEKVKEALSRIRFEAFEISAGGVRGNPPQSPRVVWSEIRDEGGCGRLF
                     DLIEDALSPLDIPRERRGYTPHATLARVKRFDPSLLPVLEGLAAGEFGSCPVRCFKLK
                     KSTLTPGGPVYEDILEVPCA"
BASE COUNT          341 a          563 c          589 g          386 t
ORIGIN      
        1 ttgggcactt cctagtgcct tgaccgcatg taagacggct acttctgcac gttttgccgg
       61 gcctgcaact gaagccttag tgccgtttcc atcgggcatc agaccatagt ataggaagta
      121 gcgaaggtcc cagtcctggc agaagggccg ggtcccgaag tactcgaggt catggatgtg
      181 aaggtctccc cccaggtgat aatcggagag gtgcgggggg agctggagga ggtactgctc
      241 cttgctgatc ttgtcagcct tcttcttgtg ggaggtctcg gcgttctcct ggaggttcgc
      301 gttgtcatgg gcctcgaagc cgcggccaac gtcgatgagg tgcgcgtcgt acaccggggt
      361 ccccacccgg gtgcagacat tccggtactc ggtgaggccc ttctcgagga gcacgatgtt
      421 cgtgatctcc cggatgaggg ggcccgagag cacctggaga cccagcttca ggatcctctg
      481 ctccacgagg acggcgatct cccgtgcgat ctcctcggtc gcgctctcgt ggccgtagaa
      541 gacctcgatg agccttgtct cctggacgat ctgccggacg atcctctccc ggtcccactc
      601 caccatgtgt ccgtccgtgg tccggacctt ggggagcgcg gggaggaacg tgccatccag
      661 cgtgagctgc tgctggaaaa cggacctgct gaccgatctc ttctgccgcg acacgggagc
      721 tcaacctctc gaaaccaata agatactatt aatccattgc tatttgattt tatcctgata
      781 tttcgcttta tatatctttt taatcgtttc cagcccatat aaatcctgca atggcccgga
      841 gaactggggt ggatcgggaa accgcgggtg catgctggcc ggccgcggga tcggggcccg
      901 ataccgggcc ggagggcgac cggagatcac ttgaggtcca gggtccggtt cagctccgct
      961 gcccttacct tccccccgga gaagagatcg gcggaggtga ggaaagtctc gtccttccgg
     1021 aggaccgggg cctccatggc aaagaccccg ttcacccgga gctcggtcag ggactccgcc
     1081 gatgacatgt ccttttcggc gaacgtgacc ctgagggaat ggaggtaccc cttcagcatc
     1141 tcgcagttcg ggcagttctc cagggtgtag acggtaagaa ctggtttctc actcatggta
     1201 tcgggccccc tcgatcgaat atccttttca ggtgatgttc caagtattct tgatggtcag
     1261 gacttttatt gctattgatc tcaccccgga ggtgagggag cggatgaggg gcccgcaggc
     1321 atcccttgca ggctgccggg cccggctcac cctcgtgaaa cctccctcca tccacatcac
     1381 cctcaagttc ctgggggaga tcgacgaaaa aaccctcgaa aaggtcaagg aggccctctc
     1441 ccggatacgc ttcgaggcct tcgagatctc cgccggcggg gtgcggggga atccgccgca
     1501 gtccccccgg gtcgtctggt cggagatccg ggacgaagga ggctgcggga ggctctttga
     1561 cctcatcgag gatgccctct cccccctgga catcccgagg gagaggaggg ggtacacccc
     1621 ccatgccacc ctcgcgcggg tgaaaaggtt cgatccctcc ctcctccccg tcctcgaagg
     1681 ccttgccgcc ggggagttcg ggtcctgccc tgtccgttgc tttaaactga agaagtctac
     1741 cctcacaccc ggaggacccg tctacgaaga tatcctggag gtgccctgcg cgtgaggaaa
     1801 cggcgggatg aagggatcgg ggccccgggg aagaagggga tccgcgccgc ggaggcgaaa
     1861 gggatccggg ccgggggcg
//
LOCUS       DATGDI010000150         7117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_393571, whole genome shotgun sequence.
ACCESSION   DATGDI010000150 DATGDI010000000
VERSION     DATGDI010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393571"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VKO45_05605"
     CDS             <1..1122
                     /locus_tag="VKO45_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HMA05386.1"
                     /translation="WTTDSELAKRAECGGAVTTFLRQALEQKLVDAVLAVKRGQDIYD
                     AVPTYITDPKNLTGTAGSLHCGTLLLSKVLARHLKGAKDKKIALTVKGCDLMGILELA
                     KRNAINRDNLYIVGVNCGGTVSPIKAMKMIQEVYQVDPMKVHKEEIDKGQFIIEYEGG
                     HKGISMDELEEKGFGRRTNCRRCEYKVPRQADLACGNWGVIGDKAGKATFVEVCSDKG
                     ADLLNRAVKAGALATEAANPKGIELRQKVEGAMLKLGEKWRRKDFTALRADMWGTIGR
                     ETARCIKCYSCIENCPVCFTSADYLKGKSFMVTAGEVPPNPMFHLRRFAHISDTCVNC
                     GQCEELCPMDIPLALFSHAIRVEGDAMFEPKLGKPAYTN"
     gene            1249..1728
                     /locus_tag="VKO45_05610"
     CDS             1249..1728
                     /locus_tag="VKO45_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2124 family protein"
                     /protein_id="HMA05387.1"
                     /translation="MKQHEVFESVPGILRPFKAYIDGLKLGAGDQIVYHGVPGTCTPF
                     IELLGFAIRSSPATQVFVPLTDESKAKKIVPVSDVGMQVSQTGVKVNPKVIVLMGGLS
                     MPNSNVTADQMKATISKYRVPLVGVCFMKMFEKTGWTKTFDFSLLIDANIAPVDVWK"
     gene            1999..3099
                     /locus_tag="VKO45_05615"
     CDS             1999..3099
                     /locus_tag="VKO45_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HMA05388.1"
                     /translation="MTDEGMPEVPVNEKTAEGVPAVEKNAFCVVIGPNGALRQESQTC
                     QPFLDMVKGSHLAWIDYVVEDVERDLLETATRLGFSELLIRNLQKNPRSGYEDFNTEL
                     GLMIPAIFMQGFDVRLEPLFILIRGNSIVTLHTREVKRFFRMRRYAETLMRKIPEGLP
                     LPDKITHLLIRIIDENNSRNFDYLVEIEEHGDQLSQELSDPKTPRSIIGPKIYEMKHA
                     LIVYLGGLWGTVDTLSSLRYGDAELMTDDEKILDRINALIGEVQAQIGLAEHLSEVLA
                     SGLEVLQSIYNNQLQILNNKLAMLVAYLTIIGTALLVPNTIATVAGNTMFNFGPEDVP
                     AYLSIIVISTVVATFASWWVLKRVGFLPSQFE"
     gene            complement(3184..3651)
                     /locus_tag="VKO45_05620"
     CDS             complement(3184..3651)
                     /locus_tag="VKO45_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05389.1"
                     /translation="MKERSALRWIGYGVLAVIIIVVIAYLSIGVVFSEAPAATSYPYT
                     TTYQVNFPASQVVTIGNSEIIAIPTGDQVTLSINKESSIMNVGETKTIQERRAMISIL
                     FMKILDFDFKIDATYRGRSGDATQFYLGFRTSEQVPSFLISRLLPASVQARPV"
     gene            3982..4860
                     /locus_tag="VKO45_05625"
     CDS             3982..4860
                     /locus_tag="VKO45_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMA05390.1"
                     /translation="MAQIPKEEYILETTSACPGCSSSLSLRYILKAAGPDTVLVIPAS
                     CTSVIQGMYPDTAVQVPVYNIAFAAAASCASGMKAGLRRAGRDTKVIVYAGDGGTVDI
                     GLQALSGALERETDFLYICFDNEAYGNTGMQRSGSTPLGALTTTTPAGKVHPKKEIDR
                     IVEAHHPPYQATAVSAFPIDLFRKVEKALTFRGPSFIHLLAPCPTGWRFPHGKTIEVG
                     RMAVKTAAWILWERANGVLTVSAPSKGPLLKPMPLGDYLGMQGRFRQITPELLERLER
                     EIDTRRREFLAEGVGG"
     gene            4857..5969
                     /locus_tag="VKO45_05630"
     CDS             4857..5969
                     /locus_tag="VKO45_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HMA05391.1"
                     /translation="MKIIATGDYAVAEAVKQASPGVIAAYPITPQTEIVERIAQFVAR
                     GEMACDYIPVESEHSAMAACIGASAVGVRAFTATSSQGLLYMHELLHWAAGARLPVVM
                     ANVNRAVGPPWNVWADHTDSLSQRDTGWLQVYAATAQEAYDATLMAFRIAEDERVLLP
                     VMVNLDGFLLSHTMQDLETLEPGNFIPPPHLPHAIDTAHPAAYAPLTGPKDYYRFRWD
                     MERSMRDARAVIAETEDLFAARFGRRYGVTEEYRCEDADVVVIALGTLAREAEVAVDS
                     LRNEGTRAGVMRIRWFRPFPILDLTGREVVVIDRDYSFGFGGVLARSIRATTRAEPYS
                     VIAGLGGQEVTHGDIATFVRERQPGGEHWFGVKAHV"
     gene            5962..6489
                     /locus_tag="VKO45_05635"
     CDS             5962..6489
                     /locus_tag="VKO45_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HMA05392.1"
                     /translation="MYEIRIHSRGGQGGVTAARLIATAAFRDGKHATATPFYGAERRG
                     APIVAFARIDEQPIRVYSQVMNPDLVVVLDRTLVPVVKVLKGLKAHGAVLVNSGHAVS
                     LDHMKVHRVDLTGIALDEDLVIAGEPVLDTPVLGAIARLGVISLESATKAIAEMFTDP
                     RNVSAAERAYRELVL"
     gene            6573..6824
                     /locus_tag="VKO45_05640"
     CDS             6573..6824
                     /locus_tag="VKO45_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA05393.1"
                     /translation="MKKRERLAISKPKKGASGKTGGWRTFRPVIEPDKCSKCGLCSLY
                     CPEGAISEDLQIDYEFCKGCGICAEECPKKAIRMEREER"
BASE COUNT         1371 a         2273 c         2292 g         1181 t
ORIGIN      
        1 tggaccaccg actcagagct cgcgaagcgt gcggagtgcg gtggagcggt gaccacgttc
       61 ctcaggcagg ccctggaaca gaagctggtg gatgccgtcc ttgcggtgaa acggggccag
      121 gacatctacg atgcggtccc gacctatatt accgacccga agaacctgac gggcaccgcc
      181 gggtctctcc actgcggaac gcttctcctc tccaaggtcc tcgcaaggca cctgaaggga
      241 gcgaaggaca agaagatcgc cctaaccgtc aagggctgcg acctgatggg catccttgag
      301 ctcgcgaagc ggaacgccat caaccgggac aacctctaca tcgtcggggt gaactgcggt
      361 ggcacagtca gccccatcaa ggcgatgaag atgatccagg aggtgtacca ggtcgatccc
      421 atgaaggtcc acaaggagga gatcgacaag ggccagttca tcatcgagta cgaggggggc
      481 cacaagggca tctccatgga cgagctcgag gagaagggct tcggccggcg gaccaactgc
      541 cgccggtgcg agtacaaggt tccccggcag gcggacctcg cctgcgggaa ctggggggtc
      601 atcggcgaca aggccgggaa ggcgaccttt gtcgaagtat gcagtgacaa gggggccgac
      661 ctcctgaacc gggcggtgaa ggcgggagcc ctcgccaccg aggcggcgaa cccgaagggg
      721 atcgagctcc ggcagaaggt cgagggtgcg atgctcaagc tcggcgagaa gtggcggagg
      781 aaggacttca cggcactccg ggccgacatg tggggcacca tcggccggga gacggcgagg
      841 tgcatcaagt gttactcgtg catcgaaaac tgcccggtct gcttcacctc ggcggactac
      901 ctgaagggga agtccttcat ggtgaccgcg ggcgaggtgc cgccgaaccc gatgttccac
      961 ctgcgccggt tcgcccacat ctccgatacc tgcgtgaact gcggccagtg cgaggagctc
     1021 tgccccatgg atatcccgct cgccctcttc tcccacgcca tccgggttga gggggacgcg
     1081 atgttcgagc cgaagctcgg gaaaccggcc tacaccaact gatctctttt tttcaggcac
     1141 gctctagagg cccgcggagc agggattccc tgctccggaa ccggggcctt ttttcccgcg
     1201 atcgctggaa agcgggaatt tatcatcacc acccgcccat tctcccgcat gaagcagcac
     1261 gaggtcttcg agagcgttcc cggtatcctt cgccccttca aggcgtacat cgacgggctg
     1321 aagctcggag ccggcgatca gatcgtgtat cacggggtgc ccggtacctg cacccccttc
     1381 atcgagctcc tggggtttgc catccgttcg agccccgcca cacaggtctt tgtccccctc
     1441 accgacgagt caaaggcaaa gaagatcgtt cccgtatctg acgtgggaat gcaggtatcc
     1501 cagacggggg tgaaggtgaa cccgaaggtc atcgtcctca tgggcggcct ttccatgccc
     1561 aattcgaacg tgaccgccga ccagatgaag gccacgatct cgaagtacag ggtccccctc
     1621 gtcggtgtct gctttatgaa aatgttcgaa aagacggggt ggacgaagac cttcgacttc
     1681 tcccttctta tcgacgcaaa tatcgcgccg gtggacgtgt ggaagtgatt ccgggattcg
     1741 acgggggaag accggtgaat ttccctcacc ttcatcccgg tacttctccg aaatagccgt
     1801 ggcccagacc cctgacctcg cggcacaggg ccttcatacc gtcaagagaa ggagaaagcc
     1861 gtcgccgttc cacaggagag cgcagtccga taagccccgg caggagatcc cgagggctcc
     1921 ggcggccacg gaccccggga ccgatccccg ggggtcatca cacaacaatt atcgccctcc
     1981 agagcagaca tgagctttat gacagacgag ggcatgcccg aggttccggt caacgagaag
     2041 accgccgaag gagtccctgc cgtcgagaag aacgcattct gcgtggtcat cggaccgaac
     2101 ggcgccctga ggcaggagtc ccagacctgc cagccctttc tcgacatggt gaaggggtcc
     2161 cacctcgcct ggatcgatta cgtggtggag gacgtggagc gggacctcct cgagaccgcg
     2221 acgaggctcg gcttctccga gctcctcatc cggaacctcc agaagaaccc gaggagcggg
     2281 tacgaggact ttaacaccga gctcgggctc atgatccccg ccattttcat gcagggcttc
     2341 gacgtgaggc tcgagcccct cttcatcctc atccggggga attccattgt caccctccac
     2401 acccgggagg tgaagcgctt cttccggatg cggcggtacg ccgagaccct catgaggaag
     2461 atccccgagg gactccccct ccccgataag atcacccacc tcctcatccg gatcatcgac
     2521 gagaacaact cccggaactt cgattacctg gtggagatcg aggagcacgg ggaccagctc
     2581 tcccaggagc tctcagaccc gaagaccccg aggtccatca tcggcccgaa gatctacgag
     2641 atgaagcatg ccctcatcgt gtacctgggc ggactctggg ggaccgtgga caccctctcc
     2701 tccctccggt acggggatgc ggagctcatg accgacgacg aaaagatcct cgatcggatc
     2761 aacgccctca tcggagaggt ccaggcccag atcggcctcg ccgagcacct ctccgaggtg
     2821 ctcgcaagcg gcctcgaggt cctccagtcc atctacaaca accagctcca gatcctgaac
     2881 aacaagctcg ccatgctcgt cgcctacctc accatcatcg gcaccgccct cctcgtcccg
     2941 aacaccatcg cgacggtcgc gggcaatacc atgttcaatt tcggcccgga ggatgtgccc
     3001 gcctatctca gcatcatcgt catctcgacg gtggtcgcga ccttcgcctc ctggtgggtc
     3061 ctgaagagag tcgggttcct cccgagccag ttcgagtagc cgtcacgacg ccggtgaaag
     3121 cgcctcacaa actgccggcc agccttccgg gaggctctcc ggaaaaaagt atttttcggt
     3181 atttcagacc ggccgcgcct gcacgctcgc cgggaggagc cggctgatga ggaacgaggg
     3241 tacctgttcg gaggtacgga aaccgaggta gaactgtgtc gcgtcaccgg atcggccccg
     3301 gtacgtggca tcgatcttga agtcgaagtc caggatcttc ataaagagga tggagatcat
     3361 cgccctcctc tcctggatgg tcttcgtttc tcccacattc atgatgctcg actccttgtt
     3421 gatggagagg gtgacctggt cccccgtcgg gatggcgatg atctcggaat tgccgatggt
     3481 gaccacctgg gatgccggga agttcacctg gtatgtggtg gtgtaggggt aggaggtggc
     3541 cgcgggggcc tcggagaaca ccaccccgat gctcaggtat gcaatcacta cgatgatgat
     3601 gacggccagg accccatagc cgatccaccg cagggcgctt cgctccttca cgggcttcgc
     3661 cttttccttt tccttcggtt tctcggctgc aggtttttca gccgccggcg ctggtgcagg
     3721 ggatgttcct tctccggtca tcatgagatc acaacaaaag agagggggac aggatgctat
     3781 ttaaccgttt gtgagcagga tacccggttt cggccatggt tccccggggg gaacccggag
     3841 ggcgtaagcc cgagcgagga gcacaaaggc gaagccttgg tgcgaacccc ggagggcaag
     3901 gcccgaggga ggagcaacag gcccgaaggc ctggtgcgaa ccctcatatc ctccggtgtt
     3961 caagtgaggg gcggtgaacc catggcccag atcccgaagg aggagtacat cctcgagacc
     4021 acctcggcct gcccggggtg cagctcgtcc ctctctctcc ggtacatcct gaaggcggca
     4081 gggcccgaca cggtcctcgt gatcccggcg agctgcacga gcgtgatcca gggaatgtac
     4141 ccggacaccg cggtccaggt cccggtctac aacatcgcct ttgcggccgc cgcctcctgc
     4201 gcctcgggga tgaaggccgg gctccggcgg gcgggtcgcg acacgaaggt catcgtgtac
     4261 gcgggggatg gtggcacggt ggacatcggg ctccaggccc tctcgggcgc cctggagcgg
     4321 gagacggact ttctctacat ctgtttcgac aacgaggcct acgggaacac ggggatgcag
     4381 cgatcggggt cgacccccct cggggccctg accacgacga cccccgcggg aaaggttcac
     4441 ccgaagaagg agatcgaccg gatcgtggag gcccaccacc cgccctacca ggccacggcg
     4501 gtgagcgcct tccccatcga ccttttccgg aaggtggaga aggccctcac cttccggggg
     4561 ccgtccttca tccacctcct cgccccctgc ccgacggggt ggcggttccc ccacgggaag
     4621 accatcgagg tggggaggat ggcggtgaag accgccgcct ggatcctctg ggagcgggcg
     4681 aacggggtcc tcacggtgag cgctccgtca aaggggcctc tcctgaagcc catgcccctc
     4741 ggggactacc tggggatgca gggaaggttc cggcagatca cccccgagct cctggaaagg
     4801 ctcgagaggg agatcgatac caggcggagg gagttcctcg ccgagggggt cggaggatga
     4861 agatcatcgc cacgggggac tatgccgtcg ccgaggcggt gaaacaggca tcgccggggg
     4921 tcatcgcggc ctaccctatc accccccaga ccgagatagt cgagcggatc gcccagttcg
     4981 tcgcccgcgg agagatggcc tgcgattaca tcccggtgga gagcgagcac tcggccatgg
     5041 ccgcctgcat cggggcctcg gccgtcgggg ttcgagcttt caccgccacc tcctcccagg
     5101 gcctcctcta catgcacgag cttctgcact gggccgcggg cgcccgcctc cccgtcgtca
     5161 tggcgaacgt gaaccgggcc gtgggaccgc cctggaacgt ctgggccgac cacaccgact
     5221 ccctgtcgca gcgggacacc ggctggctcc aggtctacgc agccaccgcc caggaggcct
     5281 atgatgccac cctcatggcc ttccggatcg ccgaggacga gcgggtgctc ctcccggtca
     5341 tggtgaacct ggacggcttc ctcctctccc atacgatgca ggacctggag accctggaac
     5401 ccgggaattt tatcccgccc ccccaccttc cccatgccat cgacaccgcc cacccggccg
     5461 cctacgcacc tctcaccggc ccgaaggact actaccggtt ccggtgggat atggagcgat
     5521 cgatgcggga tgcccgggcg gtcattgccg agacagagga tctcttcgcg gccaggttcg
     5581 ggcgacggta cggggtaacg gaggagtacc ggtgcgagga cgccgatgtc gtcgtgatcg
     5641 ccctcggaac cctcgcgagg gaggccgaag ttgcggttga cagcctccgg aacgaaggaa
     5701 cgagagccgg agtcatgcgc atccggtggt tccggccctt cccgatcctg gatctcaccg
     5761 ggagggaggt ggtggtcatc gaccgggact actccttcgg tttcggagga gtcctcgccc
     5821 gctccatccg tgcgacgacc cgggcagagc cgtacagcgt catcgccggg ctcgggggcc
     5881 aggaggtcac ccacggggac atcgccacct tcgtccggga gagacaaccc ggcggggaac
     5941 actggttcgg ggtgaaggcc catgtatgag atccggatcc attcccgggg tgggcaggga
     6001 ggggtcaccg ccgcccggct catcgccacc gcggccttcc gcgacgggaa gcatgcaacg
     6061 gcgaccccct tctacggggc cgagcggagg ggggccccca tcgtggcctt cgcccggatc
     6121 gatgagcagc ccatcagggt ctacagccag gtgatgaacc cggatctcgt cgtggtactg
     6181 gaccggaccc tggttcccgt cgtgaaggtg ctgaagggcc tgaaagccca cggggcggtg
     6241 ctcgtgaata gcggccatgc cgtcagcctc gaccacatga aggtgcaccg cgtggacctg
     6301 acgggcatcg ccctcgacga ggatctggtc atcgcagggg agccggtcct cgacaccccg
     6361 gtcctcgggg ccatcgcccg gctcggggtc atctcccttg aatccgctac gaaagccatc
     6421 gccgagatgt ttaccgaccc gaggaacgtg agcgcggccg agcgggccta ccgggagctg
     6481 gtcctgtgaa gggtgggaaa cgccggtccg ccgcacccag ggagaaacga ccggaaccgg
     6541 gaaagaccgg cgaaccgggt gccggtcccg cgatcaagaa gcgggaacgc ctcgccatat
     6601 cgaaaccgaa gaagggggcc tcggggaaga cggggggctg gcggaccttc cggccggtca
     6661 tcgagccgga caagtgtagc aagtgcggcc tctgcagcct ctactgcccg gagggggcca
     6721 tcagcgagga tctccagatc gactacgagt tctgcaaggg ctgcgggatc tgcgccgagg
     6781 agtgcccgaa gaaggccatc aggatggagc gggaggaacg atagaagggg gcccgggagg
     6841 cagctgccgg actccggggt ggctcctcat cactccccgg gaactctgtt ggaatgagac
     6901 ttccagcccg aggcttatga aagcctctta ttcgttggcc gagaacattc cctgatccgg
     6961 atgcagcaac ggagatccct gccgggccgg gaggtcctca cctccgaggg ggccctcggt
     7021 atggtgaagg caaccaccgc cgggctttcg gcagcggagg tggaggaacg gggggcgctg
     7081 tacgggccca acgagctcgc gggcaggaag aaacggg
//
LOCUS       DATGDI010000151         1598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_219102, whole genome shotgun sequence.
ACCESSION   DATGDI010000151 DATGDI010000000
VERSION     DATGDI010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219102"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VKO45_05645"
     CDS             complement(<1..428)
                     /locus_tag="VKO45_05645"
                     /inference="COORDINATES: protein motif:HMM:NF015884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HMA05394.1"
                     /translation="MATRKANGVERVLKRVREEKIRWIDLQFVDLVGGLQHITIPSTS
                     IGPDEFKRGIGKLDGSSIRGFKEIHESDMVMKPDPSTFAILPWYDGEHRTARMIVDVY
                     EGGTYERFTRDSRYIAQKAVQYAKDSGYDTTYWGPEIEFFV"
     gene            669..1127
                     /locus_tag="VKO45_05650"
     CDS             669..1127
                     /locus_tag="VKO45_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HMA05395.1"
                     /translation="MTPTVKLDELDLRIVALLNQDARKSYRDIAREVQASISTVSNRV
                     RKLESEGVITGYAPLIDESKIGFDVLAVVGVKIHKGRLLEVQRRIAKDERVTHVFDVT
                     GEWDSILVVRLKTTRELDGFIKRLGSMEYVENTYTQVVLNVVKEERRVLP"
     gene            1206..>1598
                     /locus_tag="VKO45_05655"
     CDS             1206..>1598
                     /locus_tag="VKO45_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HMA05396.1"
                     /translation="MAGYLNFDPNRKLEFEPGRPLGFDSGRPLEFLPNRELEFQPNRD
                     LGFGHRGVVFRGYICPICGALVAPDATNCNECGAVFEGPPTPPPPPPPAAPPAGRRRG
                     SGNRPPPAPRAPTPPAKPGISYCPYCGVQ"
BASE COUNT          301 a          525 c          484 g          288 t
ORIGIN      
        1 acgaagaact caatctccgg gccccagtag gtcgtgtcgt acccggagtc cttcgcgtac
       61 tgaacggcct tctgggcgat gtaccgggaa tcgcgcgtga atcgctcgta cgttccgccc
      121 tcgtagacgt ccacgatcat ccgcgcggtg cgatgctccc cgtcgtacca gggcaggatc
      181 gcgaacgtgg aagggtcggg cttcatgacc atgtccgact cgtggatctc cttgaacccg
      241 cggatcgagg atccgtcgag cttcccgatt cctcgtttga actcgtcggg tccgatggag
      301 gtcgagggga tcgtgatatg ctggagcccc ccgacgaggt ccacgaactg caggtcgatc
      361 cagcggatct tctcctcacg gacccgcttc agcacacgct ctacaccgtt agccttcctg
      421 gtcgccatcc gcttcctccc acggacccct ggagggggtt aggccggacg aaacgctccg
      481 tcctgccggt tctattgaag gaattgatac atatttaaac gattacggta tacggccgaa
      541 cagttgaccg gccaggagcc ggggggcggt acaatgtcgc agcaaccggg cctttttccg
      601 cgggattccg agcattcgac cggaactggc tccccattag gtggacctaa aatcggggga
      661 aaacacccat gacgccgacg gtcaagctcg acgagctgga cctccgaatc gtcgccctgc
      721 tgaaccagga cgcccggaaa tcgtatcggg acatcgcccg ggaggtccaa gcgagcatca
      781 gtacggtgtc caaccgagtc cgcaaactcg agtccgaggg cgtgatcaca ggctacgcgc
      841 cgctcatcga cgagtccaag atcggcttcg acgtcctcgc cgtcgtcggc gtgaagatcc
      901 acaagggccg gctgctcgaa gtccagcgga ggatcgcgaa ggatgagcgc gtgacccacg
      961 tattcgacgt cacgggtgag tgggattcca tcctcgttgt gcggctcaag accacccgcg
     1021 agctggacgg gttcatcaag cggctcgggt cgatggaata tgtcgagaac acgtacacgc
     1081 aggtcgtgct gaatgtggtc aaggaggaac gtcgagtcct cccctgaccg ggggaataaa
     1141 gggagttcct acataaccta tatcatgcca gaacccccca ttgtactcac ccggggtgga
     1201 gggacatggc agggtacttg aacttcgacc cgaaccggaa gctcgagttc gaaccggggc
     1261 gtcccctcgg tttcgattcg ggccgacccc tggaattcct gccgaaccgc gagctggagt
     1321 tccagcccaa ccgtgacctc gggttcgggc accgcggcgt cgtgttccgc ggctacatct
     1381 gccccatctg cggagccctc gttgcccccg acgctacgaa ctgcaacgaa tgtggcgcgg
     1441 tcttcgaagg cccgccgacc ccgcctccac cgccgccccc ggccgctccg cccgcgggcc
     1501 gtcgacgcgg gtccgggaat cggcctccgc cggccccgag ggcgccgacg cctcccgcca
     1561 agccgggcat ctcctactgc ccctattgcg gcgtgcaa
//
LOCUS       DATGDI010000152        14482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_364200, whole genome shotgun sequence.
ACCESSION   DATGDI010000152 DATGDI010000000
VERSION     DATGDI010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14482
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364200"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /gene="rpsK"
                     /locus_tag="VKO45_05660"
     CDS             <1..114
                     /gene="rpsK"
                     /locus_tag="VKO45_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HMA05397.1"
                     /translation="AIRALARAGMRIGRIEDVTPVPHDSCRPKGGRRGRRV"
     gene            117..947
                     /locus_tag="VKO45_05665"
     CDS             117..947
                     /locus_tag="VKO45_05665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HMA05398.1"
                     /translation="MEITFVRLADNAARFYLAGAWPALANSLRREMISGVPTLAIDDI
                     RIYDNTSVLFDEMLAHRLGLIPLRTELSTHVPKDQCSCQGAGCPACTATYTLSVEGPR
                     MVYSRDLIPQDTSAGPVDENIPIVKLIEDQKVVVEAKAHLSTGREHAKWQATTACGYK
                     NYPVIAIDEACDGCGMCVDECPRGVLGKEGEKAVVLEGHLEDCSLCRLCERACLSASI
                     GGKMSIRVSPDPERFIFSVEGDGSLPVMEILSTAVENLRKKAIDLETIISDVSGKEAP
                     "
     gene            944..1309
                     /locus_tag="VKO45_05670"
     CDS             944..1309
                     /locus_tag="VKO45_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HMA05399.1"
                     /translation="MTRTNGKTNPRLLTLITRLKETSRKNDAGIWSDIADRLEAPSRN
                     YAEVNVSKISRYARPGETFIVPGKVLGTGIITQPVVIAALNFSAGATQKIEKAEGTCL
                     SIEELLKKNPKGNGVRILR"
     gene            1314..1745
                     /locus_tag="VKO45_05675"
     CDS             1314..1745
                     /locus_tag="VKO45_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HMA05400.1"
                     /translation="MVTIIDADGMILGRMASILATRLLAGEEIAVVNAEKAIISGTRA
                     RVFSLYRHKRERGSRGWGPHFPRRPDHIIKRTIRGMVPYKRERGIEALKRVKVYVGIP
                     RDFQGKEMETLEAASVKRLSTPQFVTLGTVSKFLGASFGRE"
     gene            1749..2150
                     /locus_tag="VKO45_05680"
     CDS             1749..2150
                     /locus_tag="VKO45_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HMA05401.1"
                     /translation="MVKIINTSGKRKTAVARATFKAGQGRIRINSVPLEIYGTELARM
                     KISEPLTLLPMAMEGIDATIEVRGGGITGQAEAVRTALARGILAWHNDPKIKDTYLGY
                     DRTLLVNDSRQKESKKPHGRGARKKFQKSYR"
     gene            2182..2370
                     /locus_tag="VKO45_05685"
     CDS             2182..2370
                     /locus_tag="VKO45_05685"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HMA05402.1"
                     /translation="MIPVRCFTCGKVISTVWEEFKTRRDAGEDPARVLTDLGVERYCC
                     RRMLLSHKEIIDDLNPYQ"
     gene            2371..2445
                     /locus_tag="VKO45_05690"
     tRNA            2371..2445
                     /locus_tag="VKO45_05690"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2407..2409,aa:Pro,seq:cgg)
     gene            2492..2668
                     /locus_tag="VKO45_05695"
     CDS             2492..2668
                     /locus_tag="VKO45_05695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HMA05403.1"
                     /translation="METYTRYERARIIGARALQISMGAPVLIKSERTDPLEIAIEEFD
                     SEMIPITVKRRVGI"
     gene            2674..3861
                     /locus_tag="VKO45_05700"
     CDS             2674..3861
                     /locus_tag="VKO45_05700"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HMA05404.1"
                     /translation="MTDIELIRMRTILDSRGNPTVEAEVWTESGFGRAAAPSGASTGA
                     YEAKARPAGEAVEDAQARLIPSLIGEDAADQTGFDELLREVDGTPDFSSIGANVSTAL
                     SLANARAAASAMGLPLFTYLGGIFVTGTPLPLGNVIGGGAHAPGATEIQEFLVVPTGA
                     ADAREAVFANAAVHRRVKETLSTRGITCGKGDEGAWAPQIDDRTAFDVVREAVETVSD
                     DMKISINMGIDVAASQLFRDGRYRYRETDRSTEDQIAYMAELIDAYGLVYVEDPLHED
                     DFSGFADLTRQVNDRCLVCGDDLFVTSTERIMTGIEAGSADCVLIKPNQVGTLTDTFE
                     AVKLAHRHGMETVMSHRSGETNDDTIAHLATAFGCIFLKCGVVGGERTAKLNELIRIE
                     EIL"
     gene            3858..4469
                     /gene="rpsB"
                     /locus_tag="VKO45_05705"
     CDS             3858..4469
                     /gene="rpsB"
                     /locus_tag="VKO45_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HMA05405.1"
                     /translation="MTQANEMEIELKEPLAPVEEYLAAGVHIGTQQKSKDMKRYIYRV
                     RGDGLYILDIRATDERIKTAAKFLAQYEPPKVLIVTSRQYGQYPAKKFAEAIGAMSVT
                     GRFIPGMLTNQNIPGYLEPEVVMVTDPIGDAQVVTEAVQSGIPVVALCDTNNMTGYID
                     LVIPTNNKGRKALSLIYYLLTKEMLRLRGVATNLTLEDFETEI"
     gene            4494..5282
                     /gene="amrB"
                     /locus_tag="VKO45_05710"
     CDS             4494..5282
                     /gene="amrB"
                     /locus_tag="VKO45_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HMA05406.1"
                     /translation="MKVRSCSVAGQFYPRDPHHLEQLVAGYLKAGKVVEGVRGIVSPH
                     AGYPYSGPVAGVAFAAVDPGFSGTFVVVGPSHRGFLTCASAIPWETPLGMVDVDAEFV
                     GELGIEVDEFSHQGEHSLEVQMPFIKHRFPRARIAPVMMGDQGLRSASDLAEKIGRAQ
                     RRTRREIRIVASSDFSHYVPEEVAKMNDRYAIEALKNLDTDEFFRRIEERGVSACGYG
                     PIAALCLAAREAGATRGELLTYRTSGDVTGDLGEVVGYAAIAVM"
     gene            5284..6153
                     /gene="mvk"
                     /locus_tag="VKO45_05715"
     CDS             5284..6153
                     /gene="mvk"
                     /locus_tag="VKO45_05715"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HMA05407.1"
                     /translation="MATWSAPGKIFLFGEHAVVYGRPGLAMAIQHRVSVTVRKKRQPG
                     RVKSPYINECLGAMGVRAMVYVRSQLPSSSGLGSSAAVTVATLCAINDEFGKGLTREE
                     VARQAFEIEKKVQQGRASATDTFVSSFGGVVFIRGTEKRRLPPVNLPIVVGNSRVSHN
                     TSLMVERVGKERERHPGIVDPILDAIGALTQESLLRLHDLKALGNLMNTNHALLDALG
                     VGHPALSRLVLAARSAGAFGAKTTGAGGGGCMIALTPPRAQGRVAGAIRSCDAQAIVT
                     ILDTEGARREKNV"
     gene            6146..6898
                     /locus_tag="VKO45_05720"
     CDS             6146..6898
                     /locus_tag="VKO45_05720"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HMA05408.1"
                     /translation="MSSILLKLGGSVITDKAGDCAIRADRIAACAGEIARRPGVGVIL
                     VHGAGSCGHPQAHRHRLDRGIPPGDVAGIAETHSAVARLNAVVVDSLRAAGREAIGIH
                     PLGHCVAVDGRLRDPDWRPVGLLLALGVTPVLHGDVVTDTARGASIVSGDQLVASLAR
                     ALSPDRVGLATDVPGVLGKDGKVLGRITPGQGAGLAGGSRNTDVTGGMEGKLAELLAL
                     AEEGTGSSIFHASRIGDFLDGRPHGGTTIGGG"
     gene            6905..7972
                     /gene="fni"
                     /locus_tag="VKO45_05725"
     CDS             6905..7972
                     /gene="fni"
                     /locus_tag="VKO45_05725"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HMA05409.1"
                     /translation="MEEENSTSSRKGDHLRICAEGRVEAGFSGFSDVRLVHAALPECS
                     LGEIDTGVRFLGHRLSSPLFIAAMTGGHRDALPVNRNLAAAAERFGIGMGVGSQRAAL
                     EDPDLEDSFTVVREAAPRAFLCANLGVVQLRDHGIEWAERAVEMIDAQALCIHANFLQ
                     EAIMPEGDHDARGCLAAIGTLCRDLGTPVIVKETGAGISREMGARLWAAGVSAIDIGG
                     LGGTSWARVECIRSPESLSARMGGKFVDWGIPTVVSLREVAGNGPVIATGGVRSGLDM
                     AKALALGADLCGMALPLLAPAMESEEAVTSVLEGFTKELAVAMFLSGARTVKSMRSVR
                     TWITGTTRQMIERIQEISDGN"
     gene            7962..9302
                     /locus_tag="VKO45_05730"
     CDS             7962..9302
                     /locus_tag="VKO45_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase J family beta-CASP ribonuclease"
                     /protein_id="HMA05410.1"
                     /translation="MEIEVIAVGGYDEVGRNMTAVRCGKEIVVFDMGLRLDQVMIHED
                     AEIENMHSLDLIQMKAIPDDTMMNTVEGTVRAIVCSHGHLDHIGAITKLAHRYNAPIV
                     ATPYTIELIRQQIAGEQKFGVNNKLFVLRSGQKYTISPTLTLEFVRMQHSIIDTVMPV
                     LHTPQGAIVYSNDFKLDRTPVIGEPPDFARLRQIGKEGVLVLFGESTNVHMSGRCPSE
                     RVARDVVRDTMTSYEDDKNAILVSTFSSHIARVKTIAECAHEIGRKPVLLGRSMERYS
                     STAEQMKLVGFPQTLSMFGNRRTVERTMRRMMKMGKEKFVPIVTGHQGEPGSILTRIA
                     LDDTPYKIDKGDKILFSAKVIPNPMNFGQRYMVETRLRMKGARIFEDLHVSGHAYRED
                     HYELLHLLDPQHIIPSHGGMEQTGAYTEFAEEAGYTFQKDLHILRNGQRMILAR"
     gene            9310..10266
                     /locus_tag="VKO45_05735"
     CDS             9310..10266
                     /locus_tag="VKO45_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMA05411.1"
                     /translation="MQLTEYLHGVAEDVDLAIHHHFGNPYGELAKASAHLLTAGGKRL
                     RPAMLFLAADSVKKGSAFDLMPAALAVELIHSFTLIHDDIMDEDEERRGVPTVHTLWD
                     EPTAILAGDVLYAQAFEFLCRTPAPDTARVRAVSLLAHTCAEICDGQHRDMAFESEEE
                     VSEVEYLEMVRKKTGSLYAAAASMGAILAGGKPMQADALYQFGLTTGIAFQIQDDLID
                     LTVPSSVSGKDRGSDLREGKKTLIAIRAREKGMDLPRGPLSEAGVEKVVKSLEEAGVI
                     ADVRETAERILGTGKACLGVIPESEEKQLLLSLGDHFLARGS"
     gene            10268..11959
                     /locus_tag="VKO45_05740"
     CDS             10268..11959
                     /locus_tag="VKO45_05740"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HMA05412.1"
                     /translation="MAEDLDILVRKYALQNAVKYGGKPQAGTVVSRIMGEYPELRSEG
                     RKISVLAKAAVTEVSSLSPEKQREELARIAPELLRELSETREHVKAGLPALEGAEKGV
                     VMRFAPNPSGPLHLGHARAALLNDAYVRRYGGKYILRIEDTDPRRVDPDAYTTVREDC
                     AWLDLLIDDIVFQSDRLDTYYEHARKLIEIGGAYVCTCEADQFRELKLAKKPCPCREI
                     SPEKNLELFEAMLDGEYGEGEVTLRVKTDIEHPDPAVRDFSALRIVSSPPHPRVDATV
                     FPLMNFSVAVDDHLLGITHVIRGKDHIANTARQRYIFDYFGWKQPVYRHYGRMGIEGV
                     VLSTSQMKAGIAEGTFTGWNDIRLGTLRAIARRGITPEAVRNAVLEIGIGETDISFSW
                     ENLYAKNRDIVDPVANRYSFVPDPVLLEIEDAPCGFTAQAMLHPNDASRGYRNLPFDG
                     SVFIPKADLVCPGMIRLKDLFNITLGEGKDCMVAKFAGKSLDEARKAKAPIVQWVPPA
                     HTIPCVLHKPDGDLRGYCEPMVALDAGKVVQFERVGFARIEKATGMLIEAYWGHR"
     gene            12095..12280
                     /locus_tag="VKO45_05745"
     CDS             12095..12280
                     /locus_tag="VKO45_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05413.1"
                     /translation="MYIDRHLCCDYRVCLEMCSMGVFGIVGDMVYPVRMHLCITCLKC
                     NGFCPTHAISTRWTLRA"
     gene            12455..12772
                     /locus_tag="VKO45_05750"
     CDS             12455..12772
                     /locus_tag="VKO45_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05414.1"
                     /translation="MSQKQIFEIPDDPGCVVFYAGREISIDGKTIVELELASACSRHL
                     MTDGARAYCEKHCFNPVFVYSPGNLPVLIGFVFDEKSFNSFSYVPRTAVAGLKRPALC
                     ISS"
     gene            complement(12801..13571)
                     /locus_tag="VKO45_05755"
     CDS             complement(12801..13571)
                     /locus_tag="VKO45_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05415.1"
                     /translation="MTFLSRHPLLVTALILAAILIALVFLFGWTLRTGSESHEFQYRV
                     SISTESTLENVTLLLPVPFVYHTSSLGEALVKGEGYGIPPDWRLSLEWVNDTPMMRIF
                     AGKIEPEYHGYPIPLEQGSTPIATPPPAATAYSNETPVLIPLDFGITQTVQRSIDTQN
                     PINREPMLSNPELLKPIPCQNQPGRCYQYLVPVYVRYSSVEAGNLTISISDGGMNQWW
                     VLGWSGNSYGDTVEVIPKDTRQGWIQAEGILSTGLGRY"
     gene            13740..14126
                     /locus_tag="VKO45_05760"
     CDS             13740..14126
                     /locus_tag="VKO45_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05416.1"
                     /translation="MSGKIVGRRIISGYHMGGPLKLESTIESKGKILGQDVTFVATFW
                     QMERPQGGMFAKGHGVLMTKDGGKAVLRGAGITTAMKGGGWSSRGSRYAQTTVPALKK
                     LNEAALVFELEISPDGTVKDKWYEWK"
     gene            complement(14215..14397)
                     /locus_tag="VKO45_05765"
     CDS             complement(14215..14397)
                     /locus_tag="VKO45_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05417.1"
                     /translation="MKERAYCHGSGGAMYGLGFIGALVYYLTTATSLLGGFIGFLKAL
                     VWPAFLVYGVLKFLGM"
BASE COUNT         2763 a         4596 c         4702 g         2421 t
ORIGIN      
        1 gccatccggg cccttgcccg ggcaggcatg cggatcggga ggatcgagga cgtgaccccc
       61 gtgccccacg acagctgccg accgaaggga ggccggcggg gaaggagagt ctagacatgg
      121 agatcacctt tgtccggttg gcggacaacg ccgcccggtt ctacctcgcc ggggcctggc
      181 cggccctcgc gaactccctg cggagggaga tgatctcggg cgtcccgacc ctcgccatcg
      241 acgatatcag gatctacgac aacacgagcg tcctcttcga tgagatgctc gcccaccggc
      301 tgggcctcat ccccctccgg accgagctct ccacccatgt cccgaaggac cagtgctcct
      361 gccagggtgc gggctgtccc gcctgtacgg ccacctacac cctctcggta gaggggcccc
      421 ggatggtgta ctcgcgggac ctcatcccgc aggacacgag cgcggggccg gtcgacgaga
      481 acatccccat cgtcaagctg atcgaggacc agaaggtggt cgtcgaggcg aaggcgcacc
      541 tctccacggg gagggagcat gccaagtggc aggcgaccac ggcctgcggg tacaagaact
      601 acccggtcat cgccatcgac gaggcctgcg acggctgcgg gatgtgcgtg gacgaatgtc
      661 cccggggagt cctcgggaag gaaggggaaa aggcggtggt cctggagggc cacctcgagg
      721 attgttccct ctgccgcctc tgcgagcggg cctgcctctc cgcatccatc gggggaaaga
      781 tgtccatccg ggtgagcccg gaccccgaga ggttcatctt ctcggtggag ggcgacgggt
      841 ccctcccggt gatggagatc ctctccaccg ccgtcgagaa cctcaggaag aaggcgatag
      901 acctcgagac catcatctcc gatgtctccg gtaaggaggc accatgacga gaacgaacgg
      961 gaagaccaat ccgcgcctgc tcaccctcat cacccggctg aaggagacct cccggaagaa
     1021 cgatgcgggc atctggagcg acatcgccga ccggctggag gccccgagca ggaactacgc
     1081 cgaggtgaac gtgagcaaga tcagccggta cgcccgtccg ggcgagacct tcatcgtccc
     1141 ggggaaggtc ctcgggaccg gcatcatcac ccagccggtg gtcatcgcgg ctctcaactt
     1201 ctctgcgggc gcgacgcaga agatcgagaa ggccgagggg acctgcctct ccatcgagga
     1261 gctcctgaag aagaacccca aggggaacgg cgtgaggatc ctcaggtgac accatggtga
     1321 ccatcatcga tgcagacggg atgatcctcg gaaggatggc ctccatcctc gcgacgaggc
     1381 tcctcgccgg cgaggagatc gccgtcgtga acgccgagaa agccatcatc tcgggaaccc
     1441 gggcccgggt cttctctctc taccggcaca agagggagcg cggctcgcgg gggtggggac
     1501 cccacttccc ccggcggcct gaccacatca tcaagcggac catccggggg atggtgccct
     1561 acaagcggga gcgggggatc gaggcgctga aaagggtgaa ggtgtacgtg ggaattccga
     1621 gggatttcca gggcaaggag atggagaccc tggaggccgc ctcggtgaag aggctctcca
     1681 cgccccagtt cgtgaccctc gggacggtct cgaagttcct cggggcatcc ttcgggaggg
     1741 agtaaggcat ggtgaagatc atcaatacga gcggcaagcg caagacggcg gtggcgaggg
     1801 ccaccttcaa ggcgggccag gggaggatcc ggatcaactc ggttcccctc gagatctacg
     1861 ggaccgagct cgcccggatg aagatctccg agcccctgac cctcctcccg atggccatgg
     1921 agggcatcga cgcgaccatc gaggtccgcg ggggaggcat caccggccag gccgaggcgg
     1981 tccggaccgc cctcgcccgg gggatcctcg cctggcacaa cgacccgaag atcaaggaca
     2041 cgtacctcgg gtacgaccgg acgctcctcg tcaacgactc gcggcagaag gagtcaaaga
     2101 agccccacgg ccgcggggcg aggaagaagt tccaaaagtc ttatcggtga ggggcggaga
     2161 cttaatgaga gaccgatcgc catgataccc gtgcgatgct ttacctgtgg aaaggtgata
     2221 tccacggtct gggaggagtt caaaacgcgg cgggacgccg gggaggaccc tgccagggtc
     2281 ctcaccgacc tcggtgtgga acgctactgc tgccggagga tgctcctctc ccacaaggag
     2341 atcatcgacg atctcaatcc ataccagtga ggggtcgtgg ggtagcctgg accatcctgc
     2401 ggcgttcggg atgctgtgac ctgagttcaa atctcagcgg ccccatcggg aaggcggaaa
     2461 catcgaaaag actgaacgga gtggagggaa gatggagaca tatacccggt acgaacgggc
     2521 gaggatcatc ggggcgcggg cgctccagat ctccatgggg gcaccggtgc tcatcaagtc
     2581 ggagcggacc gatcccctgg agatcgccat cgaggagttc gactccgaga tgatccccat
     2641 caccgtgaag cgaagggtgg gaatctgacc cttatgactg acatcgagct catcaggatg
     2701 cggaccatcc tcgactcccg ggggaaccct acggtggagg ccgaggtctg gaccgagagc
     2761 ggcttcggcc gggcggcggc cccgagcggg gcatccacgg gagcctacga ggcaaaggcc
     2821 cggcctgcgg gggaggcggt ggaggacgcc caggcccggc tcatcccctc tctcatcggg
     2881 gaggacgcgg ccgaccagac cggcttcgac gagctcctcc gggaggtcga cgggacgccg
     2941 gacttctcct ccatcggggc gaacgtctcg acggccctct ccctcgcgaa tgcaagagcg
     3001 gcggcctcgg cgatggggct ccccctcttt acgtatctgg gcgggatctt cgtcacggga
     3061 acgccccttc ccctcgggaa cgtcatcggc ggcggggccc atgccccggg agcgacggag
     3121 atccaggagt tcctggtcgt ccccaccggt gccgccgatg cccgtgaggc ggtcttcgca
     3181 aacgccgccg tccaccggcg tgtgaaggag accctctcga cccgcgggat cacctgcggc
     3241 aagggggacg agggggcctg ggccccccag atcgacgacc ggaccgcctt cgacgtggtc
     3301 cgggaggcgg tggagaccgt ctccgatgac atgaagatct ccatcaacat ggggatcgat
     3361 gtcgcggcaa gccagctctt tcgcgacggc cgataccggt accgggagac tgaccggtcc
     3421 accgaggacc agatcgccta catggccgag ctcattgacg cctacggcct ggtctacgtc
     3481 gaagaccccc tccacgagga cgacttttcc ggtttcgccg acctgacccg tcaggtgaac
     3541 gaccggtgcc tcgtctgcgg ggacgacctc ttcgtcacct caactgaacg gatcatgacg
     3601 ggcatcgagg cgggatcggc ggactgcgtc ctcatcaagc cgaaccaggt ggggaccctc
     3661 accgacacct tcgaggccgt gaagctcgcc caccggcacg gcatggagac ggtcatgagc
     3721 caccggagcg gggaaacgaa tgacgatacc attgcccacc tcgccacggc ctttggctgc
     3781 atcttcctga agtgcggcgt ggtcggcggg gagcggaccg cgaagctgaa cgaactgata
     3841 cgaatcgagg agatcctatg acgcaagcga acgagatgga gatagaatta aaggagcccc
     3901 tggcgccggt cgaggaatac ctcgccgccg gcgtccacat cgggacccag cagaagagca
     3961 aggacatgaa gcggtacatc taccgggtcc ggggggacgg tctctacatc ctggacatcc
     4021 gggcgaccga cgagcggatc aagaccgccg ccaagttcct cgcccagtac gagccgccga
     4081 aggtcctcat cgtcacctcc cggcagtacg gccagtaccc cgcgaagaaa ttcgcggagg
     4141 ccatcggggc catgtccgtg accggccggt tcatcccggg tatgctcacg aaccagaaca
     4201 tccccgggta cctggagccg gaggtcgtca tggtgaccga ccccatcggg gacgcccagg
     4261 tggtgaccga ggcggtccag tcggggattc ccgtggtcgc cctctgcgac acgaacaaca
     4321 tgacgggcta catcgacctc gtcatcccca cgaacaacaa agggaggaag gcgctctccc
     4381 tgatctacta tctcctcacg aaggagatgc tccgcctccg gggtgtcgcg acgaacctca
     4441 ccctcgagga cttcgagacc gagatctagg gttcccgtgg atgggtgatg gggatgaagg
     4501 tccgctcctg cagcgtcgca ggccagttct acccccggga cccgcaccac ctggagcagc
     4561 tcgtcgccgg atacctgaag gcggggaagg tcgtcgaggg ggtgaggggg atcgtctcgc
     4621 cccatgccgg ctatccctac tcggggccgg tcgccggggt ggcctttgcg gcggtggatc
     4681 ccggtttctc cggcaccttc gtcgtcgtcg gcccgagcca ccggggcttc ctgacctgcg
     4741 cctcggccat tccctgggag acccccctcg gcatggtgga cgtggacgcc gaattcgtcg
     4801 gggagctcgg gatcgaggtg gacgagttct cgcaccaggg ggagcactct ctcgaggtcc
     4861 agatgccctt catcaagcac cggttccccc gcgcaagaat cgcaccggtc atgatggggg
     4921 accagggtct ccggtcagcc tccgatctcg ccgaaaagat cggcagggca cagaggcgca
     4981 cccggcggga gatccggatt gttgcctcca gcgacttctc ccactacgtc cccgaggagg
     5041 ttgcgaagat gaacgaccgc tatgccatcg aggcgctgaa gaacctggac accgatgagt
     5101 tcttccgtcg gatcgaggaa cggggggtct cggcctgcgg ctacggaccc atcgcggccc
     5161 tctgcctcgc ggcgagggag gcgggggcca cccgcgggga gctcctcacc taccggacga
     5221 gcggcgatgt gacgggcgac ctcggagagg tcgtgggcta cgctgccatc gcggtgatgt
     5281 aaggtggcga cctggtccgc acccgggaag atcttcctct tcggggagca tgcggtggtc
     5341 tatggccggc cgggcctcgc catggccatc cagcaccggg tctcggtgac ggtgaggaag
     5401 aagcgccagc cgggccgggt gaagtccccg tacatcaacg agtgcctggg ggcgatgggg
     5461 gtacgggcca tggtctatgt ccgttcccag ctcccgtcct cgagcggcct cgggtcctcg
     5521 gcggccgtga ccgtcgcgac cctctgcgcg ataaacgacg agttcgggaa gggcctcacg
     5581 agggaggagg tcgcccggca ggccttcgag atcgagaaga aggtccagca gggaagggcc
     5641 tccgcgacgg acaccttcgt ctcctccttc gggggggtcg tcttcatccg ggggaccgag
     5701 aagaggaggc tccccccggt gaacctcccc atcgtcgtgg gaaacagccg ggtctcccac
     5761 aacacctccc tgatggtgga gagggtaggg aaggaacggg agcggcaccc tggcatcgtg
     5821 gaccccatcc tcgatgccat cggggccctc acccaggagt ccctcctccg gctccatgac
     5881 ctgaaggcac tcgggaacct catgaacacg aaccacgccc tcctcgatgc cctcggggtg
     5941 ggccacccgg ccctctcccg gctcgtcctc gccgcccgct cggcgggggc tttcggggca
     6001 aagaccacgg gcgccggcgg cggagggtgc atgatcgccc tcacccctcc ccgggcccag
     6061 ggcagggtcg cgggggccat ccggtcctgc gatgcccagg ccattgtgac catcctcgat
     6121 acagagggag cgagaaggga gaagaatgtc tagtatcctg ctcaagctcg gggggagtgt
     6181 catcacggac aaggcgggtg actgcgccat ccgcgccgac cggatcgcgg cctgcgcggg
     6241 cgagatcgcc cggcgaccgg gggtaggggt gatcctcgtc cacggggcgg gatcctgcgg
     6301 gcacccccag gcacaccggc accggctgga ccgggggatc cctccggggg atgtcgccgg
     6361 catcgccgag acccactccg cggtcgcccg gctgaatgcc gtcgtggtgg attccctcag
     6421 ggcggccggc cgggaggcca tcgggattca tcccctgggc cactgcgtcg cggttgacgg
     6481 gaggctccgg gatcccgact ggcggcccgt cgggctcctc ctcgccctcg gggtgacccc
     6541 ggtgctccac ggcgacgtgg tgacggacac cgcccggggc gcctccatcg tctcggggga
     6601 ccagctcgtc gcgagcctcg cccgggccct ctccccggac cgggtgggcc ttgccacgga
     6661 cgtcccgggc gtactcggga aggacggaaa ggtcctcggg aggatcaccc cggggcaggg
     6721 ggccgggctc gccgggggat cccggaacac cgacgtgaca ggcggcatgg aggggaagct
     6781 cgccgagctt ctggcccttg cagaggaggg gacaggctcc tccatcttcc atgcatcccg
     6841 gatcggggac ttcctggacg ggcgcccgca cgggggcacg accataggcg gagggtagag
     6901 gaagatggaa gaagagaaca gcacatcgtc acggaaaggg gaccacctcc ggatctgcgc
     6961 cgagggccgg gtggaggcgg gcttctcggg gttctccgac gtgcgcctcg tccacgcggc
     7021 gctcccggag tgctcccttg gtgagatcga caccggggta cggttcctcg gccaccggct
     7081 ctcatcgccc ctcttcatcg cggccatgac cggcgggcac cgggacgccc tccccgtcaa
     7141 ccggaacctt gccgccgccg cggagagatt cgggatcggg atgggggtcg gctcccagcg
     7201 ggccgccctc gaggacccgg acctcgagga ctccttcacg gtggtgcggg aggcggcccc
     7261 ccgggccttc ctctgcgcga acctgggggt ggtccagctc agggaccatg ggatcgagtg
     7321 ggcggagcgg gccgtggaga tgatcgacgc ccaggccctc tgcatccatg cgaacttcct
     7381 ccaggaggcc atcatgcccg agggggatca tgacgcccgc gggtgcctcg cggccatcgg
     7441 caccctctgc agggatctcg ggaccccggt catcgtgaag gagaccgggg ccggtatctc
     7501 gagggagatg ggggcccggc tctgggccgc gggggtctcg gccatcgaca tcggcggcct
     7561 cggcgggacc tcgtgggcga gggtggagtg catccgctcg cccgagagcc tctcggcccg
     7621 gatgggcggg aagttcgtgg actggggcat ccccacggtg gtgagcctca gggaggtggc
     7681 cgggaacgga ccggtcattg ccacgggagg ggtgaggagc gggctcgata tggcaaaggc
     7741 ccttgccctc ggcgcggacc tctgcgggat ggccctcccc ctcctcgcgc ccgccatgga
     7801 atccgaagaa gcagtgacga gtgtcttaga agggttcacg aaggagctgg ccgtcgccat
     7861 gttcctttct ggggcccgga cggtgaaatc gatgagaagc gtacgaacat ggatcaccgg
     7921 gacgacccgg cagatgattg agagaataca ggagatatca gatggaaatt gaggttatcg
     7981 cggtgggcgg ctacgacgag gtcggccgga acatgaccgc cgtccgctgc ggaaaggaga
     8041 tcgtggtctt cgacatgggg cttcgcctcg accaggtgat gatccacgag gatgccgaga
     8101 tcgagaacat gcactcgctg gatctcatcc agatgaaggc aatacccgac gatacgatga
     8161 tgaataccgt agaggggacg gtgagagcca tcgtctgcag ccacggtcac ctggaccata
     8221 taggggccat cacgaagctc gcccaccggt acaacgcacc catcgtggcc acgccgtata
     8281 ccatcgagtt gatccggcag cagatcgcgg gtgagcagaa gttcggggtg aacaacaaac
     8341 tcttcgtgct caggtcgggc cagaagtaca ccatctcgcc gaccctgacc ctcgagttcg
     8401 taaggatgca gcactccatc atcgatacgg tgatgcccgt gctccacacg ccgcagggcg
     8461 ccatcgtgta ctcgaacgat ttcaagctgg accggacgcc ggttatcggg gagcccccgg
     8521 actttgcccg gctccggcag atcgggaagg agggggtcct cgtcctcttc ggcgagtcca
     8581 cgaacgtcca catgagcggg aggtgcccga gcgagcgggt cgcccgggac gtcgtgcggg
     8641 acaccatgac gagctacgag gatgacaaga atgccatcct cgtctccacc ttctcctccc
     8701 acatcgcgag ggtgaagacc atcgccgagt gcgcccacga gatcgggagg aagcccgtcc
     8761 tcctcggccg gtccatggag cgctactcct ccaccgccga gcagatgaag ctcgtcgggt
     8821 ttccccagac cctctccatg ttcggcaacc ggcggaccgt ggagcggacc atgcgacgga
     8881 tgatgaagat ggggaaggaa aagttcgtgc ccatcgtcac ggggcaccag ggggaaccgg
     8941 gctccatcct cacccggatc gccctcgacg ataccccgta caagatcgac aagggggaca
     9001 agatcctctt ctcggcaaag gtcatcccga accccatgaa cttcggccag cggtacatgg
     9061 tagagacccg gctccggatg aagggcgccc ggatcttcga ggacctccac gtctcgggcc
     9121 atgcctaccg ggaggaccac tacgagctcc ttcacctcct cgacccccag cacatcattc
     9181 cctcccacgg ggggatggag cagacagggg cctacaccga gttcgccgag gaggcgggct
     9241 acaccttcca gaaggatctc cacatcctca ggaacggcca gaggatgatc ctcgcgaggt
     9301 gaggccccca tgcaactcac cgagtacctt cacggagtgg cggaggacgt ggaccttgcc
     9361 atccaccacc acttcgggaa cccctacggc gagctcgcga aggcctccgc ccacctcctc
     9421 accgccgggg gaaagaggct ccgtcccgcc atgctcttcc tcgccgccga ctcggtgaag
     9481 aagggatcgg ccttcgacct catgcccgcg gccctcgccg tggagctgat ccactccttc
     9541 accctcatcc acgacgacat catggacgag gacgaggaga gaaggggagt tcccacggtc
     9601 cacaccctct gggacgagcc gacggccatc ctcgcgggcg acgtcctcta cgcccaggcc
     9661 ttcgagttcc tctgccggac ccccgccccc gacacggccc gggtgagggc tgtctccctc
     9721 ctcgcccaca cctgtgccga gatctgcgac ggccagcacc gggacatggc cttcgagagc
     9781 gaggaggagg tctcggaggt ggagtacctg gagatggtga ggaagaagac gggctccctc
     9841 tacgcggccg cggcctccat gggggccatc ctcgccgggg gaaagcccat gcaggcggac
     9901 gccctctacc agttcggcct caccacgggc atcgccttcc agatccagga cgacctcatc
     9961 gacctcaccg tgccttcgtc ggtgagcggc aaggaccggg gttccgacct cagggagggg
    10021 aaaaagaccc tcatcgccat ccgggcgagg gagaagggaa tggaccttcc ccgagggccc
    10081 ctgtcagagg ccggggtgga gaaggtcgtg aaatccctcg aggaggcggg ggtcatcgcc
    10141 gacgtgaggg agaccgcgga gcggatcctc ggcaccggga aggcctgcct gggagtcatc
    10201 cccgagagcg aggagaagca gctccttcta tcccttgggg accacttcct cgcacgggga
    10261 tcgtgacatg gcagaggatc tcgacatact ggtgaggaaa tacgccctcc agaacgccgt
    10321 gaagtacggc gggaagcccc aggcagggac ggtggtctcc cgtatcatgg gcgagtaccc
    10381 cgagctgcgg tcggagggac ggaagatctc ggtgctcgcg aaagccgcgg tgacggaggt
    10441 ctcttccctc tcccctgaaa agcagcggga ggagctcgcc cgcatcgctc cggagctcct
    10501 ccgggagctc tcggagaccc gggagcatgt gaaggccggc ctcccggccc tcgagggtgc
    10561 cgagaagggc gtggtcatgc ggtttgcccc gaacccgagc ggcccgctcc acctgggcca
    10621 tgcccgtgcg gccctcctga acgacgccta cgtccgccgg tatggcggga agtacatcct
    10681 ccggatcgag gacaccgacc cgcgccgggt cgaccccgat gcctacacca cggtgcggga
    10741 ggactgcgcc tggctcgatc tccttatcga cgacatcgtg ttccagagcg accggctcga
    10801 tacctattac gaacatgcca ggaaactcat cgagatcggc ggagcctatg tctgcacctg
    10861 cgaggcagac cagttccggg agctgaagct cgcgaagaag ccctgcccct gccgtgagat
    10921 ctcccccgag aagaacctgg agctcttcga ggcgatgctc gacggagagt atggagaggg
    10981 ggaggtcacc ctgcgggtga agaccgatat cgagcacccc gatccggccg tcagggactt
    11041 ctcggccctc cggatcgtct cctccccgcc gcacccgagg gtggatgcga ccgtctttcc
    11101 cctcatgaac ttctccgtgg cggtcgatga ccatcttctc gggatcaccc acgtcatccg
    11161 gggcaaggat catatcgcga acaccgcacg gcagcgctac attttcgatt acttcggctg
    11221 gaagcagccc gtgtacaggc actacgggcg gatggggatc gagggcgtag ttctctccac
    11281 ctcgcagatg aaggcgggta tcgccgaggg taccttcacc ggctggaacg acatccggct
    11341 cgggaccctc cgggccatcg cccgccgggg gatcaccccg gaggccgtcc ggaacgcggt
    11401 gcttgagatc gggatcggcg agacagacat ctccttctcc tgggagaacc tgtacgccaa
    11461 gaaccgggat atcgtcgatc ccgtggcgaa ccggtactcc ttcgtccccg atccggtcct
    11521 cctcgagatc gaggacgctc cctgcggctt caccgcacag gccatgctcc acccgaacga
    11581 tgcttcccgc gggtaccgca acctcccctt cgacgggagc gtcttcatcc cgaaggccga
    11641 cctcgtgtgt cccgggatga ttcgcctgaa ggacctcttc aacatcaccc tcggagaggg
    11701 gaaggactgc atggtcgcga agttcgccgg gaaatccctc gacgaggccc ggaaggcaaa
    11761 ggcccccatc gtccagtggg tcccgccggc ccacaccatc ccctgtgtcc tccacaagcc
    11821 cgacggggat ttaaggggct actgcgagcc gatggtcgcg ctggacgccg ggaaagtggt
    11881 gcagttcgag cgggtcgggt ttgcccggat cgagaaggcg acggggatgc tcatcgaggc
    11941 gtactggggg caccggtaga gcacccatgg gatacctatt tttacccccc gtctgaacat
    12001 cttttcacgc ccacccgcca agcgtccacc acggccaaat gggcaggagt gataccccct
    12061 ggagatctcg ccccccggca ccatcgcagt ggaggtgtac atcgaccggc acctctgctg
    12121 cgactaccgg gtatgcctgg agatgtgctc catgggggtg tttgggatcg tgggcgatat
    12181 ggtatacccg gtgaggatgc acctctgcat cacctgcctc aaatgcaacg ggttctgtcc
    12241 gacccatgcc atctccaccc gttggactct cagggcgtga ggtgccctga aaatccagcg
    12301 attcctccgg gatcgggaag aatcgggatt tcatgcccta tggcacgggc gaaggaatcc
    12361 cacccggttt cgggggtgcc cctgccctgc cacaccgtgt cacagatgtg attagtttta
    12421 tatatcgcct gcaataaaat aataaaagca gagtatgtca cagaagcaga tcttcgagat
    12481 ccccgatgac cccggctgcg tggtcttcta cgcggggcgg gagatctcca tcgacggaaa
    12541 gaccatcgtg gaactcgagc tcgcgagcgc ctgctcccgg catctcatga ccgacggtgc
    12601 cagggcgtac tgcgagaagc actgcttcaa cccggtcttc gtatattccc cggggaacct
    12661 ccccgtcctc atcgggttcg tcttcgatga gaaatcattc aactcttttt cttacgttcc
    12721 tcgcactgct gttgcgggtt tgaaacgtcc ggcactatgc atatcttcct gatggaatta
    12781 ccaccgattt cttcgttttt ttaatatcga ccaagccctg ttgagaggat tccctcggcc
    12841 tggatccatc cctgcctggt atccttcggg attacttcaa ccgtatctcc gtaagaattg
    12901 cctgaccacc ccagtaccca ccactggttc attcctccat cggagattga tatggtcaga
    12961 ttccctgcct cgacggatga ataccggaca tagaccggaa ccaggtattg gtaacatcgt
    13021 ccaggctggt tctggcaggg tatcggtttt aacaattccg gatttgagag catcggttcc
    13081 cgattgatcg ggttttgagt atcgatcgat cgctggacag tctgggtaat cccaaagtcc
    13141 aacgggatca ggaccggtgt ctcattggaa taggctgttg ccgcaggtgg cggagtcgcg
    13201 atcggcgtgc ttccctgctc gagggggatg gggtacccgt gatattcagg ctctattttt
    13261 ccggcaaata tcctcatcat gggagtgtca ttcacccatt cgagggacaa cctccaatcg
    13321 ggagggatcc catacccttc tcctttcacc agggcctctc caagggagga ggtatggtag
    13381 acgaaaggca cggggaggag cagggtgaca ttttcgaggg tggattcggt tgaaatggac
    13441 acccggtatt gaaactcatg agattcgctc ccggttctca gcgtccaacc gaataaaaaa
    13501 accagggcga tgaggatcgc ggccagaatc agggcggtga cgaggagagg gtgtctcgag
    13561 aggaacgtca tgtttgccaa ggctgatcac accggtttca attacagctc ctgaattcca
    13621 tttctttccc gggagggact taacactttc aatttttata attcgccgat atcgttatcc
    13681 gtcccgaccg tgtccgtcca tccggtgaaa cctatatgct cggcgaaatg ctcggagagt
    13741 tgagcggaaa gatcgtcgga agacgcatca tttcggggta ccacatgggt ggacccctga
    13801 agctggagag caccatagag tcaaagggga agatcctcgg ccaggacgtg acattcgttg
    13861 ccaccttctg gcagatggag cggccccagg gagggatgtt tgcgaagggc catggggtcc
    13921 tcatgaccaa ggatggtggg aaggccgtcc tccgcggggc ggggatcacc accgccatga
    13981 agggcggtgg atggagctcg aggggctccc gttatgccca gaccaccgtg cccgccttaa
    14041 agaagctcaa cgaggcggca ctcgtctttg aactcgagat ctcccccgat gggacggtca
    14101 aggacaagtg gtacgagtgg aagtgaggaa gagagagtaa caaaaggttt tcaatttttc
    14161 atttttatct ggttcatttc tgcgacgcag gcctccttgg tgctttcctt cgagttacat
    14221 cccgaggaat ttcagcaccc cgtataccag gaaagcgggc cagacgagtg ctttcaggaa
    14281 tccgatgaat cccccaagca ggctcgtggc cgtggtgagg taatacacca gggcaccgat
    14341 gaagcccagc ccgtacatcg cgcctccgga tccgtggcag tacgcccgct ctttcatagt
    14401 actcttgaaa tcacagcatt ctttcgtacc ttgctcggat gtttcgttct ccatttcact
    14461 cacctctgaa tctgacatta cc
//
LOCUS       DATGDI010000153         5378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_219355, whole genome shotgun sequence.
ACCESSION   DATGDI010000153 DATGDI010000000
VERSION     DATGDI010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219355"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="VKO45_05770"
     CDS             complement(<1..130)
                     /locus_tag="VKO45_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
                     /protein_id="HMA05418.1"
                     /translation="MAEKKSAASGWPISKGDFISGDANSCVAVVTMGSHLDEKGLCD"
     gene            complement(137..424)
                     /gene="mtrB"
                     /locus_tag="VKO45_05775"
     CDS             complement(137..424)
                     /gene="mtrB"
                     /locus_tag="VKO45_05775"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit B"
                     /protein_id="HMA05419.1"
                     /translation="MGYILALPEFGLVVDPLVGVLSSAGESLQPIIDRVATLETVADD
                     LVNMLSGEGNFLASFPGREKVLPVAGGISTFWYGLAVGLFIAGVMALLFVR"
     gene            complement(426..1280)
                     /gene="mtrC"
                     /locus_tag="VKO45_05780"
     CDS             complement(426..1280)
                     /gene="mtrC"
                     /locus_tag="VKO45_05780"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit C"
                     /protein_id="HMA05420.1"
                     /translation="MTVKIEVKEGGTPHNTIMWAGLLASLVCLYLGTGLNLAFNAYTF
                     SFFGGLAAVAAIIWGSNTIKTLCSYGIGTGVPSTGYTSFGAGVIAMLLASRVSWYLAP
                     IVLVIVAAIIGVILGYLANNVIMMKIPVLIKSTVELVMVGALVLMGLALMTTSSYSLV
                     SMVSKQTSLLGLASASYGGSFIGISIIAVAFILGMIAVQHPFNATLGPSWQQDRMLML
                     AAEVGFLTMIPVALMSIAYLSASAVIVSLVVSVAGWLYTYNQYLCLSQRDAAVWLDAK
                     PIREPEGH"
     gene            complement(1290..2126)
                     /gene="mtrD"
                     /locus_tag="VKO45_05785"
     CDS             complement(1290..2126)
                     /gene="mtrD"
                     /locus_tag="VKO45_05785"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit D"
                     /protein_id="HMA05421.1"
                     /translation="MTVTPKPTGGVGMDPVANALSIILMIAIIGAAWILFGGYTLVFK
                     ALIAVILGGALVGFGVHFVPVGGAPAAMGQAPGIATGVAMLAAGAGLAGLFGGAFAVP
                     LGLVTAIVAGGIGGALMMAITCLFVTLIYTYGMGIPSASGKVKVDPITGDSQAEYKSQ
                     GTEGHGLPFSSFAGGVIGGFMGGLGGTLIYYALLKVYEAKLPTLLSASASAVVPVAVS
                     LAGIFAIGMFLVNAVLAAYNITGTTEGFHDPKFARFPRAIFASLAASAVCGIVAILVG
                     AL"
     gene            complement(2123..3019)
                     /gene="mtrE"
                     /locus_tag="VKO45_05790"
     CDS             complement(2123..3019)
                     /gene="mtrE"
                     /locus_tag="VKO45_05790"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit E"
                     /protein_id="HMA05422.1"
                     /translation="MVENIVIGIGITAFAGALASVAGAAEDVESDIGSQGDPNSQVQL
                     APQMGYIHRIFNKAISGEPPAYGLWVALGAGLAAAFMQVNANPILALVLGCAIATLVQ
                     GIYATTAYLGRTASLAKFGQPVYIDILKSMTTVTMAHAFIAIFTAVTMVYLMNAALGH
                     PFPLALLGLIWGIALGAAGSATGNPFYGKERQYQTQKFGAGVPVAASGNIVRYAEAGE
                     RNSLDNGWFTAKLGGPASGICFGLIVFLELWRTVLFEPFWAGWGAIIVGVILILIFTF
                     IDRYAEVWARETFGPYAKEAAK"
     gene            complement(3315..5006)
                     /gene="mcrA"
                     /locus_tag="VKO45_05795"
     CDS             complement(3315..5006)
                     /gene="mcrA"
                     /locus_tag="VKO45_05795"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     alpha"
                     /protein_id="HMA05423.1"
                     /translation="MAKIERAQKLFLKSLKTRFAAWGEDVQSGKTVYKRIGLSQSPRK
                     MEWVKQGNAVALARGISGYDPVRCHCGGIPLGQRQLMTYEVSGTGVFVEGDDLHFVNN
                     AAMQQFWDDIRRTVVVGMDLAHQTLQKRLGKEVTPETINEFLHVLNHAMPGAAVVQEH
                     MVETSPALTDDCYVKVYTGDDEMADSLEPQFLIDINKQFPAKQAEQIKAAIGKSMYQA
                     VHIPTIVSRTCDGGTASRWSAMQLGMSYIGAYRMCAGEAATADLAFAAKHAGLIEMAN
                     LLPARRARGPNEPGGLKFGLFSDIVQADRKYPNDPVKSSLEVVAAGAMLFDQIWLGSY
                     MSGGVGFTQYATAAYTDNVLDDFTYYGADYVKDKYKGFTKAPQKQEVVNDIATECTLY
                     GMEQYEQFPSLLEDHFGGSQRASVLAAAAGLNCSMATGNSNAGLNGWYLSMLLHKEGW
                     SRLGFYGYDLQDQCGSANCMAIRSDEGLITELRGANYPNYAMNVGHQGEYAAIAGAAH
                     YARQDGWSFDPRIKVCFADPSLPFDFTEVRRQFAKGAIREFMPAGERSLIIPARK"
     gene            complement(5018..>5378)
                     /gene="mcrG"
                     /locus_tag="VKO45_05800"
     CDS             complement(5018..>5378)
                     /gene="mcrG"
                     /locus_tag="VKO45_05800"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050524 - coenzyme-B
                     sulfoethylthiotransferase activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     gamma"
                     /protein_id="HMA05424.1"
                     /translation="EKIAKELVSTECFDPARSGIRGCTVHGHSLRLDEDGMMFDMLAR
                     RILDKGTVKYVKDQVGVPLDKPVNVGKPMDEKWLKAHTTIYNSNGGTGFREDKEAIEF
                     IQTIHSLRVKYGFKPME"
BASE COUNT         1022 a         1616 c         1737 g         1003 t
ORIGIN      
        1 cgtcacagag tcccttctcg tcgaggtggg agcccatggt gacgaccgcg acgcagctgt
       61 tggcatcgcc gctgatgaag tcccccttgg agatgggcca tccgcttgct gccgatttct
      121 tttctgccat tgcccatcac ctcacgaaga gcagggccat tacccctgcg atgaagagtc
      181 cgactgcgag gccgtaccag aaggtactga tgccaccggc aaccgggagc accttctccc
      241 tgccggggaa ggatgcaagg aagttgccct ccccggagag catgttcacc aggtcgtcgg
      301 ccacggtttc gagggtggcc acccggtcga tgatcggttg cagcgactca ccggccgagg
      361 agaggactcc gacgagggga tcgacgacca gcccgaactc gggcagtgcg agaatgtatc
      421 ccatatcagt gtccctccgg ctccctgatg ggttttgcat cgagccatac cgccgcgtcc
      481 cgctgcgaga ggcagaggta ctggttgtac gtatacagcc agcccgccac cgatacgacg
      541 agcgagacga ttaccgctga cgcactgagg tacgcgatgg acatgagcgc gaccgggatc
      601 atggtaagga acccgacctc ggcagcgagc atgagcatcc tgtcctgctg ccagctcggt
      661 cccagggtcg cgttgaacgg gtgctggacc gcgatcatgc cgaggatgaa ggccacggct
      721 atgatggaga tgccgatgaa cgagccgcca tagcttgccg aggcaagccc gaggaggctc
      781 gtctgtttcg ataccatgct cacgagagag tagctgctcg ttgtcatcag ggccagcccc
      841 atgagcacga gagcgccgac catgacgagc tcgacggtag acttgatgag caccgggatc
      901 ttcatcatga tcacgttgtt cgccaggtac ccgaggatca ccccgatgat ggctgcaacg
      961 atcacgagga cgatgggcgc caggtaccag gaaacacggc tcgcgaggag catggcgatg
     1021 acaccagcac caaaggacgt gtaccccgtg gaggggacac cggtccctat cccgtacgaa
     1081 cagagggtct tgatggtgtt actcccccag atgatggcag cgaccgccgc gagccccccg
     1141 aagaacgaga aggtgtaggc attgaacgcg aggttcaggc ctgtgcccag gtagaggcag
     1201 acgagggatg cgaggagtcc ggcccacata atggtgttgt ggggggttcc gccttcctta
     1261 acttcaattt tgacagtcat ctttccacct cacagtgctc cgaccaggat cgccacgata
     1321 ccgcagacgg cggaggcagc caggctcgcg aatattgccc gcgggaaccg agcgaacttg
     1381 gggtcatgga acccttcagt ggtgcccgtg atgttgtacg cggcgaggac cgcgttcacg
     1441 aggaacatcc cgatggcgaa gatccctgcg agagagacag caactggcac gacagccgac
     1501 gccgaggccg acaggagtgt cggaagcttt gcctcgtaca ccttcaggag ggcatagtag
     1561 atgagcgtcc ccccgaggcc tcccatgaac cctccgatta caccgccggc gaaggaggag
     1621 aacgggagcc cgtgcccctc ggtgccctgg gacttgtact cggcctggga gtcgcccgtg
     1681 atggggtcga ccttgacctt ccccgaggcc gagggaatcc ccatcccgta ggtgtagatg
     1741 agggtgacaa agagacaggt gatggccatc atgagcgccc cgccgatgcc gcccgcgaca
     1801 atggcggtca cgaggccgag gggaaccgcg aaggctccgc cgaagaggcc cgcgaggcct
     1861 gcaccggccg cgagcatcgc gacacccgtc gcgatccccg gtgcctgccc catggcagcg
     1921 ggagcgccgc cgacagggac gaagtgcacg ccaaagccga cgagtgcacc gccgaggatg
     1981 acggcaatga gggccttgaa taccagggtg tagccgccga agaggatcca ggctgcaccg
     2041 atgatcgcga tcatgaggat gatcgagagg gcgtttgcca cgggatccat gccgacgcca
     2101 ccggttggtt tcggtgttac ggtcatttcg ccgcctcctt tgcatagggt ccgaaggttt
     2161 cccgggccca gacctcggca taccggtcga tgaacgtgaa gatgaggatc aggatgacac
     2221 cgacgataat ggcgccccag ccggcccaga agggctcgaa gagcaccgtc ctccagagct
     2281 cgaggaagac gatgaggccg aagcagatcc ccgaggccgg cccgccgagc ttcgcggtga
     2341 accagccgtt gtccagggag ttcctctcgc cggcttccgc gtaccggacg atgttccccg
     2401 aggcggccac cggcacgccg gcgccgaact tctgggtctg gtactgccgc tctttgccat
     2461 agaacgggtt ccctgttgcg gatcctgcgg ctccgagggc gataccccag atgaggccga
     2521 ggagagcgag cgggaacggg tggcctaatg cggcgttcat caggtatacc atcgtcaccg
     2581 cggtgaagat ggcgatgaag gcatgggcca tggtcacggt ggtcatcgac ttgaggatgt
     2641 cgatgtacac cggctgtccg aacttggcga ggcttgcggt cctccccagg tacgccgtgg
     2701 tcgcgtagat gccctgaacc agggtggcaa tggcgcaacc aagcacgagt gcaagtatcg
     2761 gattcgcatt cacctgcatg aatgccgcgg caagccctgc accgagggcg acccagaggc
     2821 cataggcggg gggttcaccg gatatagcct tattgaagat ccggtggatg taccccatct
     2881 ggggtgcgag ctggacctgc gagttcgggt ccccctggga cccgatatcg gactccacgt
     2941 cctccgcagc tccggcgacg cttgccaggg caccggcaaa agcggtgatt ccgatgccaa
     3001 tgacaatgtt ttcaaccatt cagcatcctc cctgcgtgca tccgcacgag agcaatataa
     3061 accgtttgcg gttcacccta acgttggtga gggttgagtt aaaggtttcg aaaagttccg
     3121 tcggacgcgt tttttcggga aatctcgcga atttgcaggg atttacgcat gctcactttc
     3181 gttaacacaa gtttaattga gttataggca accacccttg aacccgtcgg atcgagtttg
     3241 tcggtgactc cggatcggcc gattcgatgc agacttcggg agtccgtctc ccgaaaaaaa
     3301 gatttgggtt gggtttactt ccgggcaggg atgatgagcg agcgctcgcc tgcgggcatg
     3361 aactcgcgga tggcgccctt tgcaaactgc cggcgcactt cggtgaagtc gaacggaagc
     3421 gatgggtcgg cgaagcagac cttgatgcgc gggtcgaagg accagccgtc ctggcgggca
     3481 tagtgggccg cgcctgcgat ggctgcgtac tcgccctggt gaccgacgtt catcgcatag
     3541 ttcgggtagt tcgcgccacg gagctccgtg atcaggccct cgtcggaccg gatggccatg
     3601 cagttcgcgg aaccgcactg gtcctgcagg tcgtatccgt agaacccgag ccggctccag
     3661 ccttccttgt ggaggagcat ggagaggtac cagccgttca ggccggcgtt cgagttcccg
     3721 gtggccatcg aacagttcag gccggcagcc gccgcaagga cggaagcccg ctgggaaccg
     3781 ccgaagtggt cctcaaggag tgacgggaac tgctcgtact gctccatacc gtagagcgtg
     3841 cactcggtcg cgatgtcgtt gacgacctcc tgtttctggg gtgccttcgt gaagcccttg
     3901 tacttgtctt tcacgtagtc ggcaccatag taggtgaagt cgtcgaggac gttgtccgtg
     3961 taggccgcgg tggcgtactg ggtgaacccg acgccgccgg acatgtagga cccgagccag
     4021 atctggtcga agagcatcgc gcctgccgcg acaacctcga gcgaggactt gacggggtcg
     4081 ttcgggtact tccggtccgc ctggacgata tcagagaaga gaccgaactt cagtccgccg
     4141 ggctcgttcg ggccgcgggc ccgccgtgcg ggcagcaggt tggccatctc gatgaggccg
     4201 gcgtgctttg cggcaaatgc gaggtcagcg gtcgctgcct cacctgcgca catccggtac
     4261 gcaccgatgt aggacatgcc gagctgcatg gcagaccagc ggctcgcggt accgccatca
     4321 caggtcctcg acacgatggt cgggatgtgg acggcctggt acatggactt gccgatggct
     4381 gccttgatct gctcggcctg cttggccggg aactgcttgt tgatgtcgat gaggaactgg
     4441 ggctccaggg agtccgccat ctcatcgtcg ccggtgtaca ccttgacgta gcagtcgtcg
     4501 gtgagggccg gcgaggtctc gaccatgtgc tcctggacca cggccgcacc gggcatcgcg
     4561 tggttgagca cgtggaggaa ctcgttgatg gtctcggggg tgacctcctt gcccagacgc
     4621 ttctgcaggg tctggtgggc gaggtccatg ccgacgacca cggtccgccg gatgtcgtcc
     4681 cagaactgct gcatcgcggc gttgttcacg aagtgcaggt catcaccctc gacgaagact
     4741 ccggtcccgg agacctcgta ggtcatgagc tgcctctgcc cgagcgggat accaccgcag
     4801 tggcaccgga ccgggtcgta cccggagata ccccgggcga gagcaacggc gttgccctgc
     4861 tttacccact ccatcttcct cggggactgg gaaagcccga tgcgcttgta cacggtcttt
     4921 ccgctctgga cgtcctcgcc ccaggccgca aaccgggtct tgaggctctt caggaagagt
     4981 ttctgggctc tttcgatctt tgccatgttt cgtcacctca ctccatcggc ttgaagccgt
     5041 acttgacccg gagggagtgg atcgtctgga tgaactcgat tgcctccttg tcctcccgga
     5101 atccggtgcc gccgttggag ttgtagatgg tggtgtgtgc cttgagccac ttctcgtcca
     5161 tcggcttccc gacgttgacg ggcttgtcga ggggtacccc gacctggtcc ttcacgtact
     5221 tgaccgtccc cttgtcgagg atgcggcggg cgagcatgtc aaacatcatg ccgtcctcgt
     5281 cgagccggag ggagtgcccg tgcaccgtgc agccccggat accggagcgt gccgggtcaa
     5341 agcactcggt cgagacgagc tccttcgcga tcttttcg
//
LOCUS       DATGDI010000154         6805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_45044, whole genome shotgun sequence.
ACCESSION   DATGDI010000154 DATGDI010000000
VERSION     DATGDI010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45044"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            413..1627
                     /locus_tag="VKO45_05805"
     CDS             413..1627
                     /locus_tag="VKO45_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMA05425.1"
                     /translation="MNFGFWEIAILLLGAKIGGELFRRLRQPSLAGELLAGIAMGATF
                     ANLRGTLFFVDLAGSETVQLVAQLGIMFLILLAMISIDLSSIEREIERLVLAQAVSAV
                     IIYALLVTILPALSLTVNQTLIIWAAIFGSSTAISAKTLLTINGISTKEGQAVIGLQI
                     VNGIIELLLISSVTNVLQYSQFSVEPVLKLALMIIGTFAVMSRVGSRFINWLFNLFQV
                     LRLDEVFIALTLVLAFSAAAVTEAVGMTPYIGYMLVGVLVSRTEQSKSILKRMGDLGE
                     AFFIPVFFASLGLAVSVGALAGNVVLVLALLAVVTAIRFLAYTIPMVLLSFSLTDAVK
                     VGSGLLPMSEYGLLMLSVGLAYGVLANPLYSVLVAVFLIVNVAAPLVTGRVFRLPSYS
                     GGSMGRSEIKGW"
     gene            complement(1636..2451)
                     /locus_tag="VKO45_05810"
     CDS             complement(1636..2451)
                     /locus_tag="VKO45_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05426.1"
                     /translation="MAGMELPPNLGSKLGEVKSNLQSWSLAAWVSFWGFIPITLLALA
                     ALPQSVKEGWFILNTAHPERLSAWFLSSYTHSQLYPHLAGNLAVYFITLLMIFSFENR
                     HRRFRLLAGWSFLAVPLVSSLLTILFWGYLGVSTSGQGFSAVNGALLAYAMFIFIVWG
                     LEDRLEVFDHPEWFGGARFRFFVLQLLLTIMLVLVMVMGLLTGVFMDLGGSVSNGIAH
                     FGGYVTSLAVLFVFDERTERRRYFDTMLGLSILVGVIAYAYYLVLLVRLVTVA"
     gene            complement(2512..3654)
                     /locus_tag="VKO45_05815"
     CDS             complement(2512..3654)
                     /locus_tag="VKO45_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HMA05427.1"
                     /translation="MAYQVAIARCRDYDLDAVRSAVTSSLTHLGGMSRFVETGQRVLV
                     KPNLLAPRTPEEAVTTHPAVVQAVVEEVQKVGGKAVLGDSPGGRNIGASYERLLRRTG
                     MAGVAEATGCELVSFDDAGEVQAPEARSFRRFTVARAVLEADRVIVLPKLKTHQLTFY
                     TGAVKVLFGYIPGLLKAEHHLHTGHDAGTFSDLLLDLHSALPPTLALMDAVVGMEGQG
                     PSNGNPREIGLVLASPSCTALDYAACSLIGFDPREVQTVRKAGERGVGPRSLGEVEIL
                     GESLESVRVSGFEKPRTMVLGRIPPSVFHAIGHLVGSRPEVRPGRCISCGRCAETCPP
                     KAIRWAKGEIPSIRYRHCIRCYCCQELCPQGAVEVAYPWIRRVFSG"
     gene            complement(3721..4011)
                     /locus_tag="VKO45_05820"
     CDS             complement(3721..4011)
                     /locus_tag="VKO45_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HMA05428.1"
                     /translation="MDMITLIGFLAGGLTTASFLPQVVKTARTRSARDLSVAMLLVFL
                     IGLSLWTVYGIQVGSPPLIFTNLATIALVAAILAMKVKFREREKVCPPEQGK"
     gene            complement(4086..4970)
                     /locus_tag="VKO45_05825"
     CDS             complement(4086..4970)
                     /locus_tag="VKO45_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 8 protein"
                     /protein_id="HMA05429.1"
                     /translation="MTGKDPEPKPGETRRNEHVIEAIGKTRVVIRDGRVIEVGTPCIA
                     ECPLARKFAVPVADFTPEAIRANIENRIRSFGMCTEGREVTDTRDFVGFGASELLSSA
                     FRAGFIDAAVLACDGAGTVIAPVASLIQGIGGRMSGLVSTSPLPGVIRRIKSAGGYVV
                     DPDDASIDQVRGAEAAYELGFRKVAVTVADPETAREVRRLHPAAVIVAVHTTGMSAED
                     AASLVSCADLVTVCASRHLRELAVSALIQAGTGIPVFGFTRAGKEIILAKVREADAPV
                     LVKFERLPVTGTGPEPLQ"
     gene            5092..5853
                     /locus_tag="VKO45_05830"
     CDS             5092..5853
                     /locus_tag="VKO45_05830"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HMA05430.1"
                     /translation="MGGVRYGEIDALRGTAVVMMVIFHTVFSVSFFGIVAVDVVHGFW
                     RLFAVATATLFIAIAGVSLSISSARSLQALGASGVAWKNIRRGAGIFLVGMGITLVTW
                     LLVPGEYVLFGVLHCIGLSIVLSPLFLRLGRANIALGVVVILLAPLVGAGTGPLGLAW
                     LGIHPADFASLDYVPLVPWLGVFLAGVGLGTLLYPGGLRGFRADAGEGPHLEPVTFLG
                     RHSLPIYLVHVPVILLVLSLLVPGLAARLISLFPG"
     gene            5910..>6805
                     /locus_tag="VKO45_05835"
     CDS             5910..>6805
                     /locus_tag="VKO45_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05431.1"
                     /translation="MQRTSPASGGRVVLLLFLLVLLTGIPAAAAGFSGGTVVGYYFTG
                     EGCPHCARVNPELFGKWLTAYPGLVVVEYEVYGHPENAAVMAGWDPSYGLGLSIPVMT
                     FGRNATFIGDVPILQGVPGLLNQTLGEAGSAGGTTSLGALDIAGLQGYPRVWMGDRVL
                     VRENLTGGDTGLLRALLSGGDPAVLLSSGTYRDLGPLSISHGDLSAGFLHSARTDGWL
                     IGWNTVTGTNATATPTVTPSTTPTPGTCPQAPELTAGKIIALAAVNAINPCALAVLVV
                     ILLAIITQNPGRREKVLLAGLA"
BASE COUNT         1104 a         2285 c         2187 g         1229 t
ORIGIN      
        1 gaggaagagg tggcaggggt gatccgggtc cccgagggga ccctccagcc gccggatccc
       61 gttgcctgcg tcgagaagaa tcctccaggg cccggcctcg acgagcgtac aggtcgtatt
      121 tcccgtttcg gtgtcgtacc atccgttcgt cccgaggaac agcacccgca tgaagggtac
      181 tcgagagacg attccccata aacatgtggg gcgccgcggt gccccggaca tttccggcag
      241 tacggccatt cgccgaagtg ccccggacat gtccggcagt acggccatgc gccgcggtac
      301 ccccgcgttc agggcagcac ggcccagtca cgccctttca gggtccttca gataccttta
      361 ggtcacattt ctcccagatc tgtgaaggga aaggagagag ggtggacacc gggtgaactt
      421 cgggttctgg gagatcgcca tcctcctcct cggggcaaag atcgggggcg agctgttcag
      481 gcgcctgcgc cagccctccc ttgcaggtga gctcctggcg gggatcgcca tgggagccac
      541 cttcgcgaac ctccggggca ccctcttctt cgtggatctc gccggctccg agacggtcca
      601 gctcgtggca cagctcggga tcatgttcct gatcctcctc gccatgatct ccatcgacct
      661 ctcctccatc gagcgggaga tcgagaggct cgtcctcgcc caggcggtct ccgccgtgat
      721 catctacgcc ctcctcgtga caatcctccc ggccctctcc ctcacggtca accagaccct
      781 gatcatctgg gcggccatct tcggctcctc gacggccatt tctgcaaaga ccctcctcac
      841 catcaacggg atctcgacga aggaggggca ggcggtcatc ggcctccaga tcgtgaacgg
      901 gatcatcgag ctcctcctca tctcctcggt gacgaatgtc ctccagtaca gccagttttc
      961 cgtggagccg gtgctcaagc tggccctcat gatcatcggg acctttgcgg tgatgagccg
     1021 ggtcgggtcc cggttcatca actggctctt caacctcttc caggtgctcc ggctcgacga
     1081 ggtcttcatc gccctcaccc tcgtcctcgc gttttccgcg gcggcggtga ccgaggccgt
     1141 ggggatgacc ccctacatcg ggtacatgct cgtcggggta ctcgtgagtc gcaccgaaca
     1201 gtcgaagtcc atcttaaaga ggatggggga tctcggcgag gccttcttca tcccggtctt
     1261 cttcgcctcc ctcggcctcg ccgtctcggt gggggccctc gccgggaacg tggtcctcgt
     1321 cctcgccctc ctcgccgtgg tcaccgccat ccggttcctc gcctacacca tccccatggt
     1381 cctcctctcc ttctccctca ccgacgcggt caaggtcggt tccggcctcc tccccatgtc
     1441 cgagtacggc ctcctcatgc tctcggtggg gctcgcctac ggggtcctcg caaaccccct
     1501 ctactcggtg ctcgtcgcgg tgttcctcat cgtgaacgtc gcggcgcccc tcgtgacggg
     1561 gcgggtgttc aggttgcctt cctattcggg cggcagcatg ggaaggtcgg agatcaaggg
     1621 gtggtgacca ccggttcagg ctaccgttac cagcctcacg agaaggacca ggtagtaggc
     1681 gtacgcgata accccgacga ggatcgagag cccgagcatg gtatcgaagt acctcctccg
     1741 ctcggttctc tcgtcgaaga caaagagcac ggcgaggctc gtcacatagc ccccgaagtg
     1801 ggcgatgccg ttactcaccg aacccccgag atccatgaac acgccggtga ggagacccat
     1861 gaccatgacg aggacgagca tgatggtcag gaggagctgc aggacgaaga agcggaaccg
     1921 ggccccgccg aaccattccg ggtggtcgaa gacctccagc cggtcctcga gcccccagac
     1981 gatgaagatg aacatggcat aggcgaggag tgccccgttc accgccgaga aaccctggcc
     2041 cgacgtggac acccccaggt acccccagaa gaggatggtg aggagggagc tcaccagggg
     2101 aacggccagg aacgaccagc ctgcgaggag cctgaaccgc cggtgcctgt tctcgaagga
     2161 gaaaatcatg aggagggtga tgaagtacac ggcgaggttc ccggcaagat ggggatagag
     2221 ctgggaatgg gtatacgagg agaggaacca ggccgacagc ctttcggggt gggcggtatt
     2281 gaggatgaac cagccctcct tgacggactg ggggagggca gcgagggcga ggagtgtgat
     2341 gggaataaaa ccccagaagg atacccatgc tgcgaggctc caggactgca gattggactt
     2401 tacctcgccc agtttcgagc ccaggttggg aggcagctcc atccctgcca tggtacgggc
     2461 atcagttgag gcaggaataa aggtgccgat gtgccgcctc gggagatccc ctcaacccga
     2521 aaacacacgc cggatccacg ggtatgccac ctccacggca ccctgggggc agagctcctg
     2581 gcagcagtag cacctgatgc agtgccggta ccggatggag gggatctccc ccttcgccca
     2641 ccggatggcc ttcggcgggc atgtctcggc gcacctcccg caggagatgc atcgcccggg
     2701 acggacttcg ggccgggacc cgaccaggtg tccgatggca tggaagactg aaggggggat
     2761 ccgcccgaga accatggtcc tgggcttctc gaaaccagag acccggacgc tttccagaga
     2821 ttccccgagg atctccacct cccccaggct ccggggcccc acgccacgct cccctgcctt
     2881 acgaactgtc tggacctccc ggggatcgaa cccgatgagg gaacaggccg cataatcaag
     2941 ggcggtgcac gaggggctcg cgaggacgag gccgatctcc cgggggttcc cgttcgaggg
     3001 gccctgcccc tccatcccga caacggcgtc catgagggcc agggtcggcg ggagggccga
     3061 atggaggtcc aggaggaggt cggagaaggt gccggcatca tgacccgtgt ggaggtggtg
     3121 ctcggccttc aggaggccgg ggatataccc gaacaggacc tttaccgcgc cggtgtagaa
     3181 ggtgagctgg tgggtcttca gcttgggaag cacgatgacc cggtcggcct cgaggacggc
     3241 ccgtgccacg gtgaaacgtc ggaaggatct cgcctccggt gcctgcacct ctccggcatc
     3301 gtcgaaagag accagctcgc acccggtggc ctcggcgacc cccgccatcc ccgttctccg
     3361 gaggaggcgc tcgtaggacg ctccgatgtt tctgccgccg ggggagtctc ccaggaccgc
     3421 cttccccccg accttctgca cttcctcgac gacggcctgg accaccgcag gatgggtggt
     3481 caccgcttct tcaggagtcc ggggggcgag gaggttgggc ttcacgagga cccgctgccc
     3541 cgtctccacg aagcggctca tccccccgag gtgggtgagg gacgaagtga cggccgaccg
     3601 gaccgcgtcc aggtcgtagt cccggcacct cgcgatcgct acctggtatg ccacgggatg
     3661 ttcagcctcc tttcaggtct cccggcgccc cgggcatgtt ctgcggttct gccctctgga
     3721 tcatttccct tgttccggcg ggcagacctt ttccctttcc ctgaacttca ccttcatcgc
     3781 gaggatggcg gcgacgaggg cgatggtggc gaggttcgtg aagatcaggg ggggtgatcc
     3841 cacctggatc ccgtagacgg tccagaggga gaggccgatg aggaagacga ggagcatcgc
     3901 aaccgagaga tcccgcgccg acctggtcct cgccgtcttt accacctggg ggagaaagga
     3961 ggccgtggtg agccctcccg cgaggaatcc gatgagggtg atcatgtcca tgaagagttc
     4021 ccccggatca acgatcttcc gggtaaaaaa gggaggggcc ggggcatgaa ggtgtgcaag
     4081 gtgggctact gaaggggttc gggcccggtt cccgtgacgg gcagccgctc gaacttcacg
     4141 aggacagggg catcggcctc ccgcaccttg gcgaggatga tctccttccc ggcccgggtg
     4201 aagccgaaga ccgggatgcc ggtccccgcc tggatgagag cggaaaccgc gagttccctc
     4261 aggtgccgcg aggcgcagac ggtcacgagg tcggcgcagg agacgaggga ggcggcatcc
     4321 tctgccgaca tgcccgtggt gtggaccgcc acgatgaccg cggccgggtg aagccgcctc
     4381 acctcgcggg cggtttcggg gtctgcaacc gtgaccgcga ccttcctgaa accgagctcg
     4441 taggcggcct cggccccccg gacctggtcg atggaggcgt cgtcgggatc cacgacgtac
     4501 ccgcccgcgg atttaatccg ccggatgacc cccgggaggg gggaggtgga gacgaggccg
     4561 gacatcctcc cgccgatccc ctggatgagg gaggcaacgg gggcgatgac cgtgcccgcg
     4621 ccgtcgcagg cgaggaccgc cgcatcgatg aatcccgccc tgaatgccga cgagaggagc
     4681 tccgacgccc cgaagccgac gaagtcgcgg gtatcggtga cctcccttcc ttcggtgcac
     4741 atgccgaagg accggatccg gttctcgatg ttcgcccgga tcgcctccgg ggtgaaatcc
     4801 gcgaccggca cggcgaattt ccgtgcgagg gggcactcgg cgatgcaggg cgtcccgacc
     4861 tcgatgactc tcccgtcccg gatgaccacc cgggtcttcc cgatggcctc gatgacatgc
     4921 tcgttcctcc gggtttcgcc gggcttcggt tccggatcct ttcccgtcat gtcggatctt
     4981 ccctctcagt tcaccccggt ggtggatgaa cttgatggtg cccggccggg agcttcgggg
     5041 cacctcttca atacctctcc cggcagatcc tttccggagg ccgggcatcc catgggcgga
     5101 gtgcggtacg gggagatcga tgccctgcgg ggaaccgcgg tggtcatgat ggtcatcttc
     5161 cacacggtct tctccgtgag cttcttcggc atcgtggctg tggacgtggt gcacggtttc
     5221 tggaggctct ttgccgtggc cacagccacc cttttcattg ccattgccgg ggtctccctc
     5281 tccatcagct cggcacgatc cctgcaggcc ctgggagcca gcggggttgc atggaagaac
     5341 atcaggcggg gcgccggcat cttcctcgtc gggatgggca tcaccctcgt cacctggctc
     5401 ctcgtgcccg gggaatacgt cctcttcggg gtgctccact gcatcggcct ctccatcgtc
     5461 ctctctcccc tcttcctccg gctcgggagg gcgaacatcg ccctgggggt ggtggtgatc
     5521 ctccttgcac ccctcgtcgg cgcgggtacc gggcccctcg gcctcgcctg gctcgggatc
     5581 cacccggcgg acttcgcgag ccttgactac gtgcctctcg tcccctggct cggggtcttc
     5641 ctcgcgggag tgggcctcgg gacccttctc tatcccgggg gtctgcgcgg gttccgggca
     5701 gatgccgggg agggcccgca cctggagccc gtcaccttcc tcgggaggca ctcccttccc
     5761 atctacctcg tccacgtgcc cgtgatcctc ctcgtccttt cgctcctcgt cccgggcctc
     5821 gccgcccggc tcatctccct cttccccggg tagggcccgc ggccccggct cccgaaggca
     5881 ttaatcggat ggaggatata tcctctcagg tgcagcgcac atcccctgca tcgggcggga
     5941 gggtggtcct cctcctcttc ctcctcgtcc tcctgaccgg gatccccgct gcggcggccg
     6001 gattttccgg ggggacggtg gtcgggtatt acttcacggg cgagggctgc cctcactgcg
     6061 caagggtgaa ccccgaactc ttcgggaagt ggctcaccgc gtaccccggc ctcgtcgtcg
     6121 tggagtacga ggtgtacggc cacccggaga atgccgccgt aatggccggg tgggatcctt
     6181 cctacgggct cggcctgagc ataccggtca tgacctttgg gcggaacgcc accttcattg
     6241 gcgatgttcc catcctgcag ggggtccccg ggctcctgaa ccagaccctg ggggaggcgg
     6301 ggtccgccgg aggaacgacc tccctcgggg ccctggacat cgcgggcctt cagggctacc
     6361 cccgggtctg gatgggtgac cgggtccttg tccgggagaa tctcaccgga ggggacacgg
     6421 gtctcctcag ggcgcttctg tcgggcggcg atccggcggt tctcctctct tcggggacat
     6481 accgggatct cgggcccctc tccatcagcc atggcgacct ttccgcgggc tttctccact
     6541 ccgcccgtac cgatgggtgg ctcatcggat ggaacacggt gaccgggacg aatgccacag
     6601 ccacccccac ggtgacgccc tccacgaccc cgacgcccgg gacctgcccg caggcacccg
     6661 agctcaccgc agggaagatc atcgcccttg ccgccgtgaa tgccatcaac ccctgcgccc
     6721 tcgcggtcct cgtggtcatc ctcctcgcta tcatcaccca gaacccgggc cggcgggaga
     6781 aggtcctcct cgcaggcctc gcctt
//
LOCUS       DATGDI010000155         4172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_16268, whole genome shotgun sequence.
ACCESSION   DATGDI010000155 DATGDI010000000
VERSION     DATGDI010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16268"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            789..1730
                     /gene="xerA"
                     /locus_tag="VKO45_05840"
     CDS             789..1730
                     /gene="xerA"
                     /locus_tag="VKO45_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HMA05432.1"
                     /translation="MEKGHFFSREIPKYQYYLHMRNYSERTQKSYVEILNRFARYVWL
                     RRHRPDRITYQWKDLASARIDTEVEVANSWVSDFLSFLTSLRKYHPHTLHRIISTLSS
                     FYKFLKLQKVVEANPVADMERPKVKGKELVYMKHSQVLELLEKIPELRDRLVVRTIYA
                     TGVRVSELSGIQVKDIDFGSHTIRVKGKGDKIRIVFMDGETLEEIRAFIGDRKEGPLF
                     PGQMGKAISPRTIQFIFRKYAPPGITPHKLRHSYASELYSRSKNLKVVQENLGHSSIQ
                     TTEIYLHTDVEERKKVYDSYFPLSTEKGKDNDGKDES"
     gene            complement(1857..3812)
                     /locus_tag="VKO45_05845"
     CDS             complement(1857..3812)
                     /locus_tag="VKO45_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HMA05433.1"
                     /translation="MENRWQIVWMIVAMGIIAAVIGYGIVREQPVSPSGEEGGLSQFA
                     SAAEVRAWLAAHASTGGDTYYRSVDGVSPQVVDGAAEKNAAPSLPSLGTGSTQDHSTT
                     NVQVQGVDEADIVKNDGRYIYVISGGRLAIVDAYPAGKAKILSETPVNGTPSEIFLSG
                     DRLVVFSSGETSYPVMYRSAEMAVPPRYYRTSSHAYIYSLGDRTNPRMVRDITFSGTY
                     YDSRMIGDRVYVITNEPVYTYADDIPMPVVKDDAGTVLEPKVSYFDVPFSSYTFSTIS
                     SFSLTDDRALSAQSFLLGYTSTLYVSQENMYLAFQRPIAYPVRAQPVVANEMSATAVR
                     PQYTEESTAISRFAIGNGQVRYIGTGEVSGHLLNQFSMDESGGNLRAATTVQGYGSTG
                     SYQYNNVFVLDPGMKTIGALKYIAPDEQIYATRFMGDRLYMVTFRRMDPLFVIDLSDP
                     ANPGILGKLKIPGFSDYLHPYDGNHIIGIGKETQTNDWGGVSTSGLKLALFDVSDVNH
                     PTEVDHVQIGEAGSDSEALRDHKAFLFSKEKDLLVIPVRAVQQVPLEKAYAAYAQKVW
                     QGAYAFSVSPVKGFTLRGTVTHSDQDTSGYYWGSRDAVKRSLYIGNVLYTLSSDSLIA
                     TDLGTMSPLREIPLPYGRYGYPVPMME"
     gene            complement(3947..>4172)
                     /locus_tag="VKO45_05850"
     CDS             complement(3947..>4172)
                     /locus_tag="VKO45_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05434.1"
                     /translation="GKNFELGKEWKVRLLGKGATIQCTDIMSNDTTITCKFTIPKKPE
                     ESPAGTWSVIVTNADLAEDRLDDAFTVNEA"
BASE COUNT          896 a         1179 c         1262 g          835 t
ORIGIN      
        1 ggttccactg gcctttcatg gtattcaggt caagaatgga gggtgatctg gtcaaggggg
       61 aatcgatggc agagggtacc gggtgacacc gaatgcggtg gatcggtatg aaaggcccaa
      121 gtccatcggt agggggggag actgaatctc tgaaggttcc atcaacccgg gggagttccc
      181 cctgatgtgg cagccagggg tcccgaggaa aaaggggtag ggagacaact atctactgaa
      241 cgggaatccg gaaccacgcg gcggggactc ctgcgaagaa tccaaagggg ggcagagggc
      301 gaaaggaatc gtttgacagg attccatcgc cgatgagacg ggtatccagg gggtaaagga
      361 tccggatcct gatgaaatgc ctgcggacat ccctgagaag gtgcatacca gatggtgcac
      421 caatactcgg agccgacggc ccgataaccg gatcccggca cgggttatcc cgccttgtat
      481 attggatatg tcagattttc ttggtcggat gcgaatcccg agagaatccg gccctccgtc
      541 cgaagacagg ttttttattc aggatggcca ggagagaatc ccatcaaccc cccgggatcc
      601 tcacattcta caaggagtgg atctccagga aacgtacagc ttgatcgggc atggccgacc
      661 ccattataat gggatggaac cataggggag gatcgggtca ggatcggtca aattgttccg
      721 acgggtactt acgcagaatt gtagttctgc gcaaaccttt attaaattga ccgttcataa
      781 ttctgggcat ggaaaagggt cattttttct cccgggagat tcccaaatac caatactatc
      841 tccacatgcg gaactactcc gagaggaccc agaaaagcta tgtcgagatt ctcaaccgat
      901 tcgcccggta cgtctggctc aggcgccacc gtcccgacag gatcacgtac cagtggaagg
      961 acctggcctc tgcccgcatc gataccgagg tcgaggtggc aaactcctgg gtctctgact
     1021 tcctctcctt ccttacctcc ctccggaagt accaccccca cacccttcac cgcatcatct
     1081 cgacgctctc ctccttttac aagttcctca agctccagaa ggtggtggag gcgaaccccg
     1141 tcgccgacat ggaacgaccg aaggtgaagg gaaaggagct cgtctatatg aagcacagcc
     1201 aggtcctcga gctcctcgaa aaaatcccgg aactccgcga ccggctcgtc gtgcggacca
     1261 tctacgcaac cggagtgcgt gtctcggagc tctcggggat ccaggtaaag gatatcgact
     1321 tcggatccca taccatcagg gtgaagggga agggagacaa gatccgcatc gtcttcatgg
     1381 atggggagac cctggaggag atccgggcct tcatcggcga ccggaaggag ggcccccttt
     1441 ttcccggcca gatgggaaag gccatctccc cgaggaccat ccagttcatc ttcaggaagt
     1501 acgccccgcc ggggatcacc cctcacaagc tcaggcacag ttatgcgagc gagctctaca
     1561 gcaggtccaa gaacctgaag gtcgtccagg agaacctggg tcactcctcc atccagacca
     1621 cggagatcta cctgcatacc gacgtagagg aacgcaagaa ggtctacgac agttacttcc
     1681 ctctctcgac ggagaaagga aaggataacg acgggaagga tgagtcataa accgccggct
     1741 gcgatataaa agggggcgat ctcagggatc cccttttccc gtcggaaaag agccggggga
     1801 gagaaaagga tccgtcagcc tccgcccctc ccccgacggt gcagcccagg gatcccttac
     1861 tccatcatcg gaacggggta tccgtatctg ccgtagggga gggggatctc cctgagaggg
     1921 gacatcgtcc cgagatcggt cgcgatgagg gaatccgaag agagggtata caggacattt
     1981 ccgatgtaga gcgacctctt caccgcgtcc cgggatcccc agtagtatcc cgaggtatcc
     2041 tggtcgctgt gggtgacggt gcccctcagg gtgaatccct tcaccggtga caccgagaag
     2101 gcataagccc cctgccaaac cttctgggcg tacgcggcgt acgccttctc gagcggcacc
     2161 tgctgcaccg cccgcacggg gatcaccagg aggtccttct ccttcgagaa gaggaaagcc
     2221 ttgtggtccc ggagggcctc ggaatccgat ccggcttccc cgatctggac gtggtcgacc
     2281 tcggtggggt ggttcacatc cgagacatcg aagagggcga gcttcagccc cgacgtggag
     2341 acaccgcccc agtcattggt ctgggtttcc ttgccgatcc cgatgatgtg gtttccgtca
     2401 tagggatgga ggtaatccga gaagccggga atcttcagtt tcccgaggat gcccgggttc
     2461 gcggggtccg agaggtcgat gacaaagaga ggatccatcc gccggaaggt gaccatgtag
     2521 agccggtcgc ccatgaaccg ggtggcatag atctgctcgt cgggagcgat gtacttcagg
     2581 gccccgatgg ttttcatacc cgggtccagg acgaagacgt tgttgtactg gtaggagccc
     2641 gtgctgccat agccctgcac cgtggtggcg gccctcaggt taccgccgga ttcgtccatg
     2701 gagaactggt tcaggaggtg cccggaaacc tccccggtcc cgatgtaccg gacctggccg
     2761 ttcccgatgg cgaaccgtga gatggcggtg gattcctcgg tgtactgcgg ccggacggcc
     2821 gtcgcggaca tctcattcgc cacgacgggc tgggcgcgga ccggatacgc gatgggtctc
     2881 tggaaggcca ggtacatgtt ctcctgcgag acgtagaggg tcgaggtgta gcccaggagg
     2941 aaggactggg ccgagagagc ccgatcgtcc gtcagggaga aggacgagat ggtggagaaa
     3001 gtgtaggagc tgaaggggac atcgaagtac gacaccttcg gttcgaggac ggtccccgca
     3061 tcatccttca cgaccggcat cgggatatcg tcggcgtagg tgtataccgg ctcattcgtg
     3121 atgacataaa ccctgtcccc gatcatccgg gaatcatagt acgtccccga gaaggtgata
     3181 tccctgacca tccgtgggtt cgtccggtcg ccaagggagt agatgtatgc gtgggacgag
     3241 gtccggtaat accggggtgg cactgccatc tcggccgacc tgtacatcac ggggtaggag
     3301 gtttcccccg aggaaaagac aacgagcctg tcgccggaga ggaagatctc cgacggggtc
     3361 ccgttcaccg gggtctcgga gaggatcttt gccttcccgg ccgggtaggc gtcgacaatg
     3421 gcgagccgcc cgccggagat gacgtagatg tacctcccgt cgttcttcac gatgtcggcc
     3481 tcatccactc cctggacctg gacattcgtc gtcgagtgat cctgggtcga ccccgtcccc
     3541 agggacggga gggagggtgc agcgttcttc tccgcggccc cgtccaccac ctgggggctt
     3601 accccgtcca ccgaccggta ataggtgtca ccaccggtcg aagcgtgtgc cgcgagccag
     3661 gctcttacct ctgctgcgga ggcgaactgc gagagtccac cctcctcacc ggaaggtgag
     3721 acaggctgtt cccggactat tccatacccg atgacggccg cgatgattcc cattgcgacg
     3781 atcatccaga cgatctgcca gcggttctcc ataggatcga tccccagtct tctcaatcgg
     3841 ctgcttatac tggcttattg gtgcaaaagg atatcatatt tgccttaatt acgaaaagat
     3901 tcgtaatgat ggggggtttt agagccttaa aaggagaggg ggatgttcac gcctcgttca
     3961 ccgtgaaggc gtcgtcgagc cggtcttctg ccagatccgc gttggtgacg atcacggacc
     4021 atgtacccgc cgggctttcc tcgggttttt tcgggatggt aaacttgcat gtgatggtcg
     4081 tgtcattcga catgatatct gtacattgta ttgtcgcccc tttgccgagg agcctgacct
     4141 tccattcctt tcccagctca aagttctttc cc
//
LOCUS       DATGDI010000156         7539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_336584, whole genome shotgun sequence.
ACCESSION   DATGDI010000156 DATGDI010000000
VERSION     DATGDI010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336584"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VKO45_05855"
     CDS             complement(<1..999)
                     /locus_tag="VKO45_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HMA05435.1"
                     /translation="MQVEIGDGEKRFLLGHHRVKDPAETVGEMEPLMREAGVLDLIDC
                     TEKDRPRVPCFAVIRRRTGSVRPQVHVGRGVSPAEAQASAMATAIERYSAEYRGDPMK
                     IAGYEEIGIRRAVDPDQLILARPLEAGERIHWSPGYDLLEKEEVWVPSNAVFLPYNTL
                     GEAKGLFASDPNGLAAGNTRDEAILSGLLEVLERDSLSRAERDHSMGKRLSVTGQGPV
                     QDLLGLFEKAGIPVTLWLLDGITAVPTVAAAADDPVTKDPAFLVLGAGAHPSPRAAAI
                     QALTEVAQRRAVRIQGGSASGEREGFVQRVGYDRMKRLNREWFAPAPSVDLADLPDL"
     gene            1055..1342
                     /locus_tag="VKO45_05860"
     CDS             1055..1342
                     /locus_tag="VKO45_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2098 domain-containing protein"
                     /protein_id="HMA05436.1"
                     /translation="MESGEVAVGAHVRYPRTGTTGKVVRIERIDRDTFAELDTTHLLY
                     RTDQLISYEPKGGREERKEGPDLEQVAKEREAARSAQSQWLSADELCDGGG"
     gene            complement(1339..1761)
                     /gene="nikR"
                     /locus_tag="VKO45_05865"
     CDS             complement(1339..1761)
                     /gene="nikR"
                     /locus_tag="VKO45_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HMA05437.1"
                     /translation="MEKEPELSRIGISLPQLLLEKFDEIITIRGYSSRSEGIRDAIRA
                     YITHYQWMSDVKGERQGVITMVYDHTQRGLTQTLLDIEHDYGYVIQASLHSHVTHDRC
                     LEVILLRGEGTELKHVAERLMSQKGVESVKLTTIPVQD"
     gene            1933..3168
                     /locus_tag="VKO45_05870"
     CDS             1933..3168
                     /locus_tag="VKO45_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HMA05438.1"
                     /translation="MRDVTIPGVNIGVVGHVDHGKTTLVSALTGVWTDRHSEEIKRGI
                     SIRLGYADATFVRCDDTGNPEAYSTGALPSPCGDKAAPFRTVSFVDAPGHETLMATML
                     SGSALMDGAMLVIAANEPCPQPQTKEHLMALSMVGIRRIVIVQNKIDVVPQKEAIAHF
                     EQIKKFVQGTIAEGAPIVPVSAQKKVNIGALIQALDTAIPEPERDPSASPVMLIARSF
                     DVNKPGSSWKDLRGGVIGGSLIRGVLHEGDELEIRPGLSMQVENRTRWDPIVTEVTSI
                     QTGSKKVAEATPGGLLGVATKLDPALTKSDALAGQVAGLPGKLPPVWDRLHFQVTLME
                     RVVGSESEVNVEPLKIKEPLMLSVGTAVTVGIVGNARKDHIEAILKRPVCAETGARIA
                     ISRQVGGRWRLIGMGVLGE"
     gene            3165..3545
                     /locus_tag="VKO45_05875"
     CDS             3165..3545
                     /locus_tag="VKO45_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HMA05439.1"
                     /translation="MKVLLDTNAFLLPVQFGVDLIRELEGIFGACDLLTLDGVVRELQ
                     GIGTGQGKDAAAARVGLDLARRCRVVPSPSSGGDVDRDVVRVAEESGCVVVTNDRAVR
                     QSLLGKGIGVVTLRGRKRLEFIRG"
     gene            3555..4136
                     /locus_tag="VKO45_05880"
     CDS             3555..4136
                     /locus_tag="VKO45_05880"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HMA05440.1"
                     /translation="MYFKMKLMDKVRVPPNRLGEPLNSVVLNVLQQQLEGAIDREIGI
                     FIAVTKVLDVGEGELIPGDGAVYYDVTFEALVLRLALQEVIEGDVVETTTFGCFVSLG
                     PIDAMLHVSQISDDFINYDEKNGRLICQESKRFIGVGDAVRARVVTLSLNEREPRESK
                     IGLTMRQAGLGTQRWLAEDLEKEQEKVKSHGGA"
     gene            4123..4323
                     /gene="spt4"
                     /locus_tag="VKO45_05885"
     CDS             4123..4323
                     /gene="spt4"
                     /locus_tag="VKO45_05885"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HMA05441.1"
                     /translation="MVVRKKQANVCRQCHRVVEGEACAVCGSPMLTQDWAGYLVILDP
                     EHSEMARRLNIKLPGRYALKVR"
     gene            4323..4847
                     /locus_tag="VKO45_05890"
     CDS             4323..4847
                     /locus_tag="VKO45_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-dependent dephospho-CoA kinase family
                     protein"
                     /protein_id="HMA05442.1"
                     /translation="MHRRLPEAERTSLRRPFGQVFPDVRALLPRIRERVVYAVGDVVT
                     HNLLREGIAPRIAIIDGHTMRAPCTRVPELPFRRIVARNPAGTISDDLVRAIRDAVAD
                     PPALIFVEGEEDLAVLPLVLEAPLGAVILYGQPGEGVVLREVDPGAKALAAAFVERFV
                     HEENAGPAGRGPTL"
     gene            4881..5324
                     /locus_tag="VKO45_05895"
     CDS             4881..5324
                     /locus_tag="VKO45_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05443.1"
                     /translation="MIPTADLKITGDRRNELLKRRELTFTLGYTASTPSRSEVMGKVC
                     AILNLDEKKVVLDSVKTRFGERTMAGIARVYDDEQMLAKTERPYLMNRGKPKAKEGAE
                     AEAPKEPAKAAPKEAAKEAPAESPKEAPKAAPKHAPKEPAKEAGA"
     gene            5324..5485
                     /locus_tag="VKO45_05900"
     CDS             5324..5485
                     /locus_tag="VKO45_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HMA05444.1"
                     /translation="MAAKRSGYYTLQEGKAVLTRKHCPRCGPGVLMAEHKDRTSCGRC
                     GYTEFKKKE"
     gene            5499..7076
                     /locus_tag="VKO45_05905"
     CDS             5499..7076
                     /locus_tag="VKO45_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional N(6)-L-threonylcarbamoyladenine
                     synthase/serine/threonine protein kinase"
                     /protein_id="HMA05445.1"
                     /translation="MQKGPVLGIEGTAWNLSASVFDEDLVTLSSHAYQPPHGGIHPRE
                     AAQHHAQVMKEVIAGALRGAPPVTGVAFSQGPGLGPCLRTTATAARALALGWGVPLVG
                     VNHCVAHVEIGRFATGFSDPIVLYVSGANTQVLGFLNHRYRIFGETLDIGLGNALDKF
                     ARSRGLSHPGGPAIEALAAGGEPLDLPYTVKGMDLAFSGLVSAAKDSKAPIGDVCAGL
                     QETAFAMCVEVTERALAHAGKEEVILVGGVAANRRLQEMARIMCDERGARFAVPEPKY
                     LGDNGAMIAFTGRLMLASGQSIPIAESGILPGFRADEVEVTWRGAGEGMIIRHDIRRD
                     GTEEARGAEAVVTIGKDTVKKRRLSKSYRVPSIDARLIAERTRAEARIISQARRSGIP
                     TPVIRDVTADTLVMERIEGPLLKEVLTEENVREAGRMVGRLHTAGIIHGDLTTSNIIV
                     RNGRCVLIDFGLSLVSTEIENRGVDLHVFLQTLESTSREYVSLREAFTGGYREEFPGS
                     EEVLEREQEIRRRGRYL"
     gene            7073..>7539
                     /locus_tag="VKO45_05910"
     CDS             7073..>7539
                     /locus_tag="VKO45_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HMA05446.1"
                     /translation="MKLAVVTSNPHKAREIAAFFGGEPEVEHVNLECPEFRHSDVGEI
                     AGGKARFAFECLKRPLIVDDTSFSVQALKGFPGPYAAYVLDTIGFQGILRLLDGVEER
                     EAFFETAIAYADEWGIRIFRGRIDGVITRSPRGREGFGYDPIFEVEARTLAEIS"
BASE COUNT         1320 a         2407 c         2616 g         1196 t
ORIGIN      
        1 gaggtcgggg aggtcggcga ggtccacgga gggcgccggc gcgaaccatt cccggttcag
       61 gcgcttcatc cggtcgtacc cgacccgctg gacgaagccc tcccgctccc ccgaggcaga
      121 ccccccctgg atccgcacgg cccgccgctg ggcaacctcg gtgagggcct ggatggccgc
      181 cgcccggggc gatgggtggg caccggcccc gaggacgaga aaggccgggt ctttggtcac
      241 cgggtcgtcg gctgccgcgg ccaccgtcgg aaccgcggtg atgccgtcga ggagccagag
      301 ggtgacgggg atcccggcct tctcgaagag cccgaggaga tcctggaccg ggccctgccc
      361 ggtcaccgac agcctcttcc ccatggagtg gtcccgctct gcccgggaga gggagtcccg
      421 ttccaggacc tcgaggagcc ccgagaggat ggcctcgtcc cgggtgttcc ccgccgcgag
      481 gccgttcggg tctgaggcaa agaggccctt cgcctctccc agggtgttgt agggaaggaa
      541 cacggcatta ctcggaaccc agacctcctc cttctcgagg aggtcgtagc cgggggacca
      601 gtggattctc tccccggctt ccaggggccg ggcgaggatg agctgatcgg gatcgacagc
      661 ccgacggatc ccgatctcct cgtacccggc gatcttcatg ggatcccccc ggtactccgc
      721 cgagtaccgc tcgatggccg tcgccatggc cgaggcctgc gcctccgcgg gggagacccc
      781 cctcccgaca tggacctggg gcctcaccga ccccgtcctc cgtcggatga cggcgaagca
      841 ggggacgcgg ggccggtcct tttcggtgca gtcgatgagg tcgaggaccc cggcctcccg
      901 catgaggggt tccatctccc cgacggtctc ggccggatcc ttcacccggt ggtggccgag
      961 aaggaaccgc ttctccccgt ccccgatctc cacctgcatg aagggagaga tgggaggcca
     1021 tccttttata gtttggcgga ggggatgatc ctctatggaa tccggagagg tcgcggtcgg
     1081 ggcgcatgtc cggtaccccc ggaccggtac caccgggaag gtcgtccgta tcgagaggat
     1141 cgaccgggat accttcgcgg aactggacac cactcacctc ctgtaccgca ccgaccagct
     1201 catctcttat gagccgaagg gcgggcggga ggagaggaag gaagggccag acctcgagca
     1261 ggtggcaaag gagcgggagg cggcccgctc ggcccagtcc cagtggctct ccgccgacga
     1321 gctctgcgac ggcgggggct agtcctggac ggggatggtg gtgagcttca ccgactccac
     1381 ccccttctgg gacatgagcc gctcggcgac gtgcttcagc tccgtgccct cgccccggag
     1441 aaggatcacc tccaggcagc gatcatgggt gacatgggag tggagggacg cctggatcac
     1501 gtagccgtag tcatgctcga tgtcgaggag ggtctgcgtg agcccccgct gggtgtggtc
     1561 atagaccatg gtgatgaccc cctggcgctc ccccttcacg tcagacatcc actggtaatg
     1621 ggtgatgtag gcccggatcg cgtcccggat tccctcggac cgggatgaat agccccggat
     1681 ggtgatgatc tcgtcgaact tctcgaggag aagctgggga agggagatcc cgatccggga
     1741 gagctcgggc tctttttcca aggagaccac tgaaaagggg gttcgctggg gaagacattg
     1801 aagatttcgc tccccgggaa ccccggcggt gcaggcgccg ggcaacggga tttttccccg
     1861 ttccggcccg ccgcatctcg gatcaggtat aagtataccg gggttatatt ggttagggag
     1921 gtctgagggc atttgcggga cgtcaccatt cccggtgtga atatcggggt cgtcgggcac
     1981 gtggatcacg ggaagaccac cctcgtctcg gccctcaccg gagtctggac ggaccggcac
     2041 agcgaggaga tcaagcgggg gatctccatc cggctgggct acgcggatgc cacctttgtc
     2101 cggtgcgacg ataccgggaa tcccgaggcc tattccacgg gggccctccc ctcgccctgc
     2161 ggcgataagg ccgccccctt ccggacggtc tccttcgtgg atgcgccggg ccacgagacc
     2221 ctcatggcca ccatgctctc gggctcggcc ctcatggacg gggcgatgct cgtcatcgcc
     2281 gcgaacgagc cctgccccca gccccagacc aaggaacacc tcatggccct ctccatggta
     2341 gggatccgga ggatcgtcat cgtccagaac aagatcgatg tcgtccccca gaaggaggcg
     2401 atagcccact tcgagcagat caaaaagttc gtccagggga ccatcgccga gggagccccc
     2461 atcgtcccgg tctctgccca gaagaaggtg aacatcgggg ccctcatcca ggccctggac
     2521 acggcgatcc cggagcccga gagggacccg tcggcatccc ccgtgatgct catcgcccgg
     2581 tccttcgatg tgaacaagcc gggctcgagc tggaaggacc tgcgtggcgg ggtcatcggc
     2641 gggtcgctca tccggggggt gctccacgag ggcgacgagc tggagatccg gccgggcctc
     2701 tcgatgcagg tggagaaccg gacccggtgg gatcccatcg ttaccgaggt gacctccatc
     2761 cagacgggtt caaagaaggt cgcggaggcc acccccgggg ggctcctcgg tgtggcgacg
     2821 aagctcgacc cggcgctgac caagagcgat gccctggccg ggcaggtggc cggccttccc
     2881 gggaaactcc cccccgtctg ggacaggctt cacttccagg taaccctcat ggagcgggtc
     2941 gtcggttccg agagcgaggt gaacgtggag cccctgaaga tcaaggaacc cctcatgctc
     3001 tcggtgggaa cggcggtaac ggtggggatc gtggggaacg cgaggaagga ccacatcgag
     3061 gcgatcctga agcggcccgt gtgcgccgag acgggagccc ggatcgccat cagccggcag
     3121 gtcggcggaa gatggcgcct gatcgggatg ggagtgctgg gcgagtgaag gtgcttctcg
     3181 atacgaacgc cttcctcctc ccggtccagt tcggggtcga cctgatccgg gagctcgaag
     3241 ggatcttcgg cgcctgcgat cttctcaccc tggacggggt ggtacgggag ctccagggga
     3301 tcgggaccgg ccaggggaag gacgccgccg cggcccgcgt ggggctcgat ctcgcccggc
     3361 gctgcagggt ggttccctcg ccctcctccg gaggggacgt ggacagggac gtcgtccggg
     3421 tggccgagga atccggatgc gtcgtcgtga cgaatgaccg ggccgtcagg caatccctcc
     3481 tcgggaaggg catcggcgtg gtgaccctga ggggccggaa gcggctcgag ttcatccgtg
     3541 gataggtgac aggcgtgtac ttcaagatga agctcatgga caaggtccgg gtccccccga
     3601 acaggctggg ggagcccctg aactcggtgg tgttaaacgt actccagcag cagctcgagg
     3661 gggccatcga ccgggagatc ggtatcttca tcgccgtgac gaaggtcctc gacgtgggag
     3721 agggggagct catccccggc gacggggcgg tctactatga cgtgaccttc gaggccctcg
     3781 tgcttcgtct cgccctccag gaggtcatcg agggggatgt ggtcgagacc accaccttcg
     3841 ggtgcttcgt ctccctcggg cccatcgacg ccatgctcca cgtgagccag atctccgacg
     3901 acttcatcaa ctatgacgag aagaacgggc ggctcatctg ccaggagtcc aagcgcttca
     3961 tcggcgtggg ggacgcggtc cgggcccggg tggtcaccct ctccctgaac gaaagggagc
     4021 cccgggagtc gaagatcggc ctcaccatgc gccaggcggg cctcgggacc cagcgctggc
     4081 tcgccgagga tctcgagaag gagcaggaaa aggtgaagag ccatggtggt gcgtaagaaa
     4141 caggcgaacg tctgccggca gtgccaccgg gtcgtggagg gggaggcctg cgccgtctgc
     4201 ggctccccga tgctcaccca ggactgggcg ggctacctcg tcattctcga cccggagcac
     4261 tcagagatgg caaggcgcct caacatcaaa ctccccggcc gctacgccct gaaggtccgc
     4321 tgatgcaccg gcgcctcccc gaggccgagc ggacctccct caggaggccc ttcgggcagg
     4381 tgttccccga tgtccgagcc ctcctccccc gcatccggga gcgggtggtc tacgcggtcg
     4441 gggacgtggt cacccacaac ctcctccggg aggggattgc accccggatc gccatcatcg
     4501 acgggcacac catgcgggcc ccctgtacac gggtacccga gctccccttc cggaggattg
     4561 tcgcccggaa ccctgcaggc accatctcag acgatctcgt ccgggccatc cgcgacgccg
     4621 tcgccgatcc cccggccctc atcttcgtcg agggggagga ggacctcgcg gtcctgcccc
     4681 tcgtcctcga ggccccttta ggagcggtga tcctctacgg gcagccgggg gagggcgtgg
     4741 ttctccggga ggtcgacccc ggggcgaagg ccctcgcggc ggccttcgtg gaacggttcg
     4801 tacatgagga gaacgccggc ccggcaggca ggggccccac cctttaaata aacccggagt
     4861 caatgtatgg aggatactcg atgatcccaa cggctgatct caagataacg ggtgacagga
     4921 ggaacgagct tctcaagcga agggagctca ccttcaccct gggctacacg gcaagcaccc
     4981 cctcacggag cgaggtgatg ggcaaggtct gcgccatcct gaacctcgac gagaagaagg
     5041 tggtgctcga ctcggtaaag acccggttcg gcgagaggac catggcgggc atcgcccggg
     5101 tgtacgacga cgagcagatg ctcgcgaaga ccgagcggcc gtacctcatg aaccggggca
     5161 agccgaaggc gaaggaaggg gccgaggcag aggcgccgaa ggagcctgca aaggcagctc
     5221 cgaaggaggc cgcaaaggag gccccggccg agtccccgaa ggaggctccc aaggctgcac
     5281 cgaagcacgc gcccaaggag ccggcgaagg aggcgggtgc ctgatggcgg cgaagcgctc
     5341 cggctactac acccttcagg aagggaaggc cgtccttacg aggaagcact gcccgaggtg
     5401 tgggcccggg gtcctcatgg ccgagcacaa ggaccggacc tcctgtggca ggtgcgggta
     5461 caccgagttc aagaagaagg aataagtaca ggaataggat gcagaaaggg ccggtgctgg
     5521 ggatcgaggg cacggcctgg aacctctctg cctctgtttt tgacgaggat ctcgtcaccc
     5581 tctcttctca cgcctaccag cccccgcacg ggggtatcca cccgagggag gccgcccagc
     5641 atcacgccca ggtgatgaag gaggttatcg ccggagccct ccggggtgct cccccggtca
     5701 ccggcgtcgc cttctcgcag ggcccgggcc tcggaccctg cctccggacc accgccaccg
     5761 cggcccgggc cctggccctc ggctgggggg ttcccctcgt cggggtgaac cactgcgtcg
     5821 cccatgtcga gatcggccgg ttcgcgaccg gtttttccga tcccatcgtc ctctacgtga
     5881 gcggggcaaa cacccaggtc ctcggcttcc tgaaccaccg gtaccggatc ttcggagaga
     5941 ccctggacat cggcctcggg aacgccctgg acaagttcgc ccggagccgg ggtctttccc
     6001 accccggcgg accggccatc gaggccctcg ccgcgggggg cgagcccctc gatcttccct
     6061 acacggtgaa ggggatggac ctcgccttct cggggctcgt ctccgctgca aaggattcga
     6121 aggcgcccat cggtgatgtc tgcgccgggc tccaggagac cgcctttgcc atgtgcgtcg
     6181 aggtgaccga gcgggccctc gcccatgccg ggaaagagga ggtcatcctc gtgggcgggg
     6241 tcgcggcgaa ccgcaggctc caggagatgg cgaggattat gtgcgacgaa cggggggccc
     6301 ggtttgcggt gcccgagccg aagtacctgg gggacaatgg cgccatgatc gcctttaccg
     6361 ggaggctcat gctcgcctcc ggccaatcca tccccattgc ggaatcggga atcctcccgg
     6421 gtttccgggc tgacgaggtg gaggtgacct ggagaggagc gggggagggc atgatcatca
     6481 ggcacgacat ccggagggac gggaccgagg aggcccgggg cgccgaggcg gtggtgacca
     6541 tcgggaagga taccgtgaag aaacggcgcc tctccaagag ttaccgggtc ccgtccatcg
     6601 atgcccgcct catcgcggag agaacccgtg ccgaggcgag gatcatctcc caggctcggc
     6661 ggagcgggat cccgactccc gtcatcaggg acgtcaccgc ggacaccctg gtcatggagc
     6721 ggatcgaggg gccgctcctc aaggaggtgc tcacggagga gaatgtccgg gaggcgggga
     6781 ggatggtcgg gaggctccac accgcgggga tcatccacgg tgacctgacg acgagcaaca
     6841 tcatcgtccg gaacggccgg tgcgtgctca tcgacttcgg gctctccctc gtctcgacag
     6901 agatcgaaaa ccggggcgtc gatctccacg tgttcctcca gaccctggag agcacatcga
     6961 gggagtacgt ctctctcagg gaggccttta ccggtggata ccgggaggag ttcccgggat
     7021 ccgaggaggt tctcgagcgg gagcaggaga tccggcgccg ggggaggtac ctgtgaaact
     7081 cgcggtggtc acctcgaacc cccacaaggc aagggagatc gcggccttct tcgggggcga
     7141 gcccgaggtg gagcacgtga acctcgagtg cccggagttc cggcattccg acgtggggga
     7201 gattgcaggg gggaaggcgc ggtttgcctt cgagtgcctg aagcgtcccc tcatcgtgga
     7261 cgacacctcc ttctcggtgc aggccctcaa gggctttcca gggccgtatg ccgcgtacgt
     7321 cctggacacc atcgggttcc agggaatcct ccggcttctg gacggggtag aggagagaga
     7381 ggcatttttc gagacggcga ttgcctacgc cgatgagtgg ggcatccgta tctttcgggg
     7441 caggatcgac ggggtcatca cccggtcccc ccggggcagg gaggggttcg ggtacgaccc
     7501 catcttcgag gtagaggcga ggactctcgc cgagatctc
//
LOCUS       DATGDI010000157         8880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_191294, whole genome shotgun sequence.
ACCESSION   DATGDI010000157 DATGDI010000000
VERSION     DATGDI010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8880
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191294"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1180
                     /locus_tag="VKO45_05915"
     CDS             71..1180
                     /locus_tag="VKO45_05915"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMA05447.1"
                     /translation="MEGMRFSEKVQGIDLSGIRRIFEAAAPGSINFGLGQPDFDTPRH
                     IKDAAIRAIREGKTGYTVNAGIPELRAAICDKLRRENALSYEPGQIIVTAGGSEALLL
                     VTQSLLNPGDRVLVPDPGFVSYAALATIAGARVEGLPLTSTLHIDIDAAMERMPGARL
                     LFLNSPANPTGAVESPESICALVECAEDHGVTVVSDEVYEHFTYGKPHVSAACYGENV
                     VTVNAASKTYAMTGWRVGYLAAEESVVAQCIKVHQYCQACASSISQYAALAAYEGDQS
                     SVAMMRREYQARRDLLYEGLSRFGFQFPLPEGAFYAFVPMGRGLMDRILKKGVIIVPG
                     DSFGRNAREYARMSYATSRENIGEALTRIGSAMGG"
     gene            1182..2270
                     /locus_tag="VKO45_05920"
     CDS             1182..2270
                     /locus_tag="VKO45_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HMA05448.1"
                     /translation="MREILGRLSNAHGVSGSEGSVEDIIRAELRGSVDEIREDALGNL
                     IAVKKGGAFSVMIASHMDEIGLMVNYIDEKGFLRFVTFGGWYAPTLYNQRVILHGFKG
                     PVTGVVGGKPPHVMDEEERKRGVKAENMFIDVGASSAKEVARLGLEIGTPVTVDRELA
                     DLAGRRVTGKAFDNRVGCAVLVKVLKELKSPHTVYGVFTVQEEVGTKGARTSAFSLDP
                     DCAIALDSTFPGDHPDIDKKDSCLEMGKGPAITVSDANGRGLIADRRMVAWLRKVAEK
                     KKIPYQLEVGHGGTTDASSIHLVKSGIPSTVISVPVRYIHSPVEVADLRDMEASARLL
                     LEALKGNPKGVSPRENARVETRARTTRQ"
     gene            2390..4069
                     /locus_tag="VKO45_05925"
     CDS             2390..4069
                     /locus_tag="VKO45_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMA05449.1"
                     /translation="MKKRGLMVTILLLDAGPGFSDRSRVIASGIGDISVLFTATAAEA
                     LSRLGEGDVDLVLGYSGPGDAEAVRLLEAIRSSGKGLPFIFIADRGDTEFPTQAYTHG
                     ADLVLDRLPDSPEAWAGLNRTIRALAGRWRREENLRLENGIMAAVLNATPMAVCVIRD
                     HAIVWANRTLLSMLGYGEGELIGRDPIGFMGGEKEHRRIDDGLFGTRDEFGWGSIETQ
                     VRRRDGSLLGVHLQSRPVDPSDPSQGHIVMGQDMTEYHRIRDLLRKSELRYQDLIDNT
                     HSIILRVDPGGIIRFINRFGETFFGYSSDEIVGRHLLGTILPERSRTGRDLAAMVTEV
                     MKKPEDFEVNVNENMKKSGERVWIAWSNKAIRDEKGRLLEMLSIGNDITDRKLDVKEV
                     SITIAPWKKALIEETDIGEPVFEAAYEIAAEISREGREGKPVGTTFILGDARNVLAHS
                     RQLILNPFEGHPREARMITNPNLRETIKELAQLDGAFVVRGDGAVCAGGRYITIDTSG
                     TEIEKGLGTRHASVAGITQETRAVGIVVSQSGGRISLFKDGRVVRVISSAD"
     gene            complement(4076..4555)
                     /locus_tag="VKO45_05930"
     CDS             complement(4076..4555)
                     /locus_tag="VKO45_05930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012105998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMA05450.1"
                     /translation="MTTPTIPTQGKLVRLETTMGNITLELRPDMPITAGNFAKLAGQG
                     YYDGTIFHRVISDFMIQGGDPTGTGRGGPGYTIRDEFTPNNRNVRGTIAMANAGPNTG
                     GSQFFINLVDNPHLNTKHPVFGKVVEGMDVVDKIGRVKTDSSDRPVTPVKIVKATVL"
     gene            complement(4702..6384)
                     /locus_tag="VKO45_05935"
     CDS             complement(4702..6384)
                     /locus_tag="VKO45_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HMA05451.1"
                     /translation="MVSIRAPEWIRRLHLLQGILPLDTGQVPGEILAGITFAALAIPA
                     ALGYANIAGMPVVTGLYTLLLPMAVFALVGSSRHLVVGADSATAVIIASGLATMAAPQ
                     SPQYVAYAGMIALLSAVFLLLAGLLKLGFVSDFLSRAVIIGFLTGVGFQIALGQLGGM
                     FGMAPLSGGPVLQAVSFALHLPLTHIPTLLLSVIVIGVIALGNRVSIRIPGALIVVAG
                     AILVSWVFDLSVYGITVLGPVPGGLPAPGFPPVPLSDIVPLLNLAAACFVIILAQSAT
                     TSRAYAWRFPAAVNENDDLVGLGLANLAAGLSGTFVVNGSPTKTEMVYGAGGRTQLAQ
                     LTAVGVLLAVLLFITPPLSFLPVAVLSAVVFLIGLRLIDVRGMASLRTRRPVEFGVAL
                     VTAGTVVLIGVGQGIAIAVAASVIAHLRHSYRPLNSLLVPTPEGAIRPVPLADGQQAM
                     EGLIIYRFGADLYFANEKRLLEEILSLARDAEPPIRWFCISAANIGDVDYSGAETLKQ
                     VHDELAKRGVVLVLSDVKEPVLAKLGRDGIVELIGREYIFESAYDVIRIYGD"
     gene            complement(6541..7248)
                     /locus_tag="VKO45_05940"
     CDS             complement(6541..7248)
                     /locus_tag="VKO45_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05452.1"
                     /translation="MICLLVLFVSIAGAQGSGSLVRGNTFTVTVIGHSRTLYDIWPKG
                     THDMTGEPGDQPPVIVPGQVDVTQDPPGGPYTLGSHPIGGGGTIRDDVPPDSSTTSAT
                     AYYAEVRTDASGYGVVLFRTSSATATQQFHITAENPIDLSEDVGLVLGLPPTPAPVIP
                     LPLPTTMKTPFMPVTTRVPATPVPTATPTPVPLITPVPMETPTETSPTPAVPLPAAIG
                     VIAAGIGLLLAARVRKT"
     gene            7419..8033
                     /locus_tag="VKO45_05945"
     CDS             7419..8033
                     /locus_tag="VKO45_05945"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HMA05453.1"
                     /translation="MVSKKFYTLEDLPYAFNALEPHMSEAQLRLHHDKHHAAYVNGAN
                     AIFERLDRARQAGTDMDMKATVKELSFQAGGHILHLLFWQNLAPTAKAAKEPGGTLAE
                     ALKNEFGSFERFKKEFSSVATSTEGSGWAALARCGLTGRLLVMQIEKHNVNVYPHLRI
                     LMVLDIWEHAYYLDYKNERPRYVEAFWNIVNWDVVNNRLEALIK"
     gene            8351..8518
                     /locus_tag="VKO45_05950"
     CDS             8351..8518
                     /locus_tag="VKO45_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05454.1"
                     /translation="MKTKFVMVMDTLVWFKIVAVTWGVGVVVVTVVARAVPEGVGAVV
                     AWVVAWVVAGP"
BASE COUNT         1707 a         2666 c         2985 g         1522 t
ORIGIN      
        1 gagatcaagg agatcaacgt cgagacgctc aagggaaggc acgtgaacat cgagtgaagg
       61 gagagagggg atggaaggga tgaggttttc cgagaaggta cagggcatcg atctctcagg
      121 catccggcgg atcttcgagg cggcggcccc ggggtccatc aacttcggcc tcgggcagcc
      181 ggacttcgac accccccggc acatcaagga cgccgccatc cgggccatca gggagggaaa
      241 gacgggttac accgtgaacg ccggtatccc cgagctccgg gcggccatct gcgacaagct
      301 ccgccgggag aacgccctct cgtacgaacc cggccagatc atcgtcacgg caggggggag
      361 cgaggcgctc ctcctcgtga cccagtccct cctgaacccg ggcgaccggg ttctcgtccc
      421 cgatccgggg ttcgtctcct acgccgccct cgcgaccatc gccggcgccc gggtggaggg
      481 cctccccctc acctcgaccc tgcacatcga tatcgacgcg gcgatggagc ggatgccggg
      541 ggcccggctc ctcttcctga acagcccggc gaacccgacg ggggcggtgg agagccccga
      601 gtccatctgc gccctcgtgg agtgcgccga ggaccacggg gtgaccgtgg tctcggacga
      661 ggtgtacgag cacttcacgt acgggaagcc ccacgtgagc gcggcctgct acggggagaa
      721 cgtcgtcacc gtgaacgcgg cgtcgaagac ctatgccatg accggctggc gggtcgggta
      781 cctcgccgcc gaggagagcg tcgtcgccca gtgcatcaag gtccaccagt actgccaggc
      841 ctgcgcgagc tccatctccc agtacgcggc tctcgcggct tacgagggcg accagtcctc
      901 ggtggcgatg atgcgccgcg agtaccaggc gcggcgtgac ctcctctacg agggactgag
      961 ccggttcggg ttccagttcc cgcttcccga gggggccttc tacgccttcg ttcccatggg
     1021 gaggggactc atggaccgga tcctcaagaa gggggtcatc atcgtcccgg gcgactcctt
     1081 cggcaggaac gcgagggagt acgcgaggat gtcctacgcc acctcccggg agaatatcgg
     1141 cgaggccctg acgaggatcg ggtcggcgat gggtgggtag gatgcgcgag atcctcggga
     1201 ggctctccaa tgcccacggt gtctcgggga gcgaggggag cgtggaggac atcatccggg
     1261 ccgagctccg gggttctgtc gacgagatcc gggaggacgc cctggggaac ctcatcgcgg
     1321 tgaagaaggg gggtgccttc tcggtcatga tcgcctcgca catggacgag atcggcctca
     1381 tggtgaacta catcgacgag aagggcttcc tccggttcgt gaccttcggc ggatggtacg
     1441 cccccaccct ctacaaccag cgggtcattc tccacgggtt caagggcccc gttacgggag
     1501 tcgtgggcgg gaagccgcct catgtcatgg acgaggagga gcggaagagg ggcgtgaagg
     1561 ccgagaacat gttcatcgac gtaggagcct cctcggcaaa ggaagttgcc cggctcggcc
     1621 tcgagatcgg caccccggtc accgttgacc gggagctcgc ggacctcgcc gggcggcggg
     1681 tcacggggaa ggccttcgac aaccgggtcg gctgcgccgt cctcgtcaag gtattaaagg
     1741 agctgaagtc cccccacacc gtgtacgggg tctttaccgt ccaggaggag gtggggacca
     1801 agggggcgag gacgagcgcc ttctccctcg accccgactg cgccatcgcc ctcgattcca
     1861 ccttcccggg cgatcacccc gacatcgaca agaaggactc ctgtctcgag atggggaagg
     1921 ggccggccat caccgtctcg gacgcaaacg ggagggggct catcgccgac cggaggatgg
     1981 tcgcctggct ccggaaggtc gccgagaaaa agaagatccc gtaccagctc gaggtcggcc
     2041 acggggggac gaccgatgcc tcctccatcc atctcgtgaa gagcgggatc ccgtccacgg
     2101 tcatcagcgt gccggtccgc tacatccact cccccgtcga ggtcgccgac ctccgggaca
     2161 tggaggcctc ggcgaggctc ctcctcgagg ctctgaaggg gaacccgaag ggcgtgagcc
     2221 cgagggagaa cgcgagggtg gaaacccgag cgagaaccac caggcagtaa cctggtggtg
     2281 accaacgggg cgaagccccg gtggagaagc catccctctg atctccccct ttcctccctg
     2341 tttcgagaac cccgggatca accagcaggg taatggttcc tgaattccca tgaagaagcg
     2401 aggcctcatg gtcaccatcc ttctcctcga tgccggaccc ggtttttctg acagatcccg
     2461 ggttatcgca tccgggatcg gggatatttc ggtcctcttc accgctacag ccgccgaggc
     2521 cctttcccgg cttggggagg gggatgtgga cctggtgctc gggtattccg gccccgggga
     2581 tgccgaggcg gtccggctcc tggaggccat ccggtcttcg gggaagggac ttcccttcat
     2641 tttcattgcc gaccggggag acacggaatt cccgacgcag gcctataccc atggagcaga
     2701 tctcgtcctg gaccggttgc cggattctcc ggaagcctgg gccgggctca acaggaccat
     2761 ccgggccctc gccggccgct ggcggcgtga ggagaacctg cgcctggaga acgggatcat
     2821 ggccgcggta ctgaacgcga cccccatggc cgtctgtgtc atccgggacc atgccatcgt
     2881 ctgggcgaac cgcacgctcc tgtccatgct ggggtacggg gagggggaac tgatcgggag
     2941 ggatccgata gggttcatgg gcggggagaa ggagcaccgt cggatcgatg acgggctctt
     3001 cgggacccgg gatgagttcg gctggggctc catcgagacg caggtgaggc ggagggatgg
     3061 ctccctgctg ggcgtccacc tccagtcccg gccggttgac ccgtcggatc cctcgcaggg
     3121 tcatatcgtc atgggccagg acatgaccga gtatcaccgg atccgggacc tgctccggaa
     3181 gagcgagctc cggtaccagg acctcatcga taatacccac agcatcatcc tccgggtgga
     3241 tcccgggggg atcatccggt tcatcaaccg gttcggggag accttcttcg gctattcctc
     3301 ggatgagatt gtcgggaggc acctcctcgg gaccatcctc cccgagcgtt cgcgtacggg
     3361 gcgggatctc gccgcgatgg tgacggaggt gatgaaaaag ccggaggact tcgaggtaaa
     3421 tgtcaacgag aacatgaaga aatccggtga acgggtctgg atcgcctggt ccaacaaggc
     3481 gatccgggac gagaagggga ggctcctcga gatgctctcc atagggaacg acatcacgga
     3541 ccggaagctg gacgtgaaag aggtaagtat caccatcgcc ccctggaaga aggcccttat
     3601 cgaggagacc gacatcgggg agccggtctt tgaggcggcc tacgagatcg cggccgagat
     3661 ctcccgtgag gggcgggagg ggaagccggt ggggaccacc ttcattctcg gggatgcccg
     3721 gaacgtcctc gcccattccc ggcagctgat cctgaatccc ttcgagggac acccccggga
     3781 ggcccggatg atcacgaacc ccaatctcag ggagaccatc aaggagctcg cccagctgga
     3841 tggcgccttt gtcgtgcgcg gtgacggtgc ggtctgtgct ggcggacggt acatcaccat
     3901 cgataccagc gggaccgaga tcgagaaggg cctgggcacc cgccacgcct cggtggccgg
     3961 gattacccag gagacccggg ccgtgggaat cgtggtctcc cagagcgggg gccggatctc
     4021 cctcttcaag gacgggcgcg tggtcagggt tatctcgtcc gccgactgag gcagatcaga
     4081 gcacggtcgc cttcacgatt ttcaccggtg tcaccggccg gtcgctcgag tcggtcttca
     4141 ccctgccgat cttgtccacc acatccatcc cctccaccac cttcccgaag accgggtgct
     4201 tggtgttgag gtgcgggtta tccacgaggt tgatgaaaaa ctggcttccc cccgtgttcg
     4261 ggccggcgtt cgccatggcg atggtccccc ggacgttcct gttgttcggg gtgaactcgt
     4321 cgcggatggt atacccgggg ccaccccggc cggtgcccgt gggatcgccg ccctggatca
     4381 tgaagtcgct gatgacccgg tggaagatgg tcccgtcgta gtatccctgg ccggcgagtt
     4441 tcgcgaaatt ccccgccgtg atgggcatat cgggcctgag ttcgagggtg atgtttccca
     4501 tggttgtctc aagcctcacg agctttccct gggttggtat ggttggtgtc gtcatggacg
     4561 atgccccgtt ctgcgcctct gtgcagcctg ctgcgatgac cagcgccccg acgaggagga
     4621 gagccggcaa tatccgggac tgcatggccg gaatcttttg tctcaaacgg gattataggt
     4681 ttggatcccc ggtcccccgc gtcaatcccc gtagatccga atgacgtcgt acgccgattc
     4741 gaagatatac tccctgccga tcaactcgac gatcccgtcc cgccccagct tggcgagcac
     4801 gggctccttc acgtcggaga gcacgaggac cactccccgc ttcgcgagct cgtcatggac
     4861 ctgcttcagg gtctctgccc ccgagtagtc cacgtcaccg atgttcgcgg ccgagatgca
     4921 gaaccaccgg atcggcggct cggcgtcccg ggcgagggag aggatctcct cgaggaggcg
     4981 tttctcgttc gcgaagtaga ggtccgcacc aaaccggtag atgatcaggc cctccatggc
     5041 ctgctgcccg tccgcgaggg ggacgggccg aatggccccc tcgggggtgg gcacgaggag
     5101 ggagttcagc ggccggtagc tatgccggag gtgggcgatg acggaggcgg ccacggcgat
     5161 ggcgatcccc tgcccgaccc cgatgaggac gaccgtaccg gcggtcacga gggccacccc
     5221 gaactccacc gggcgcctcg tcctgaggga ggccatcccc cgcacgtcga tgagccggag
     5281 cccgatgagg aacacgaccg ccgagagcac ggccacgggg aggaaggaga ggggcggggt
     5341 gatgaagagg aggaccgcga ggaggacccc gacggcggtg agctgggcga gctgggtccg
     5401 tccccccgcg ccgtagacca tctcggtctt cgtggggctc ccgttcacga cgaaggtccc
     5461 cgagaggccg gccgcgaggt tcgcgaggcc gaggcccacg agatcgtcgt tctcattcac
     5521 cgcggcgggg aacctccagg cataggcccg ggaagtcgtc gcgctctggg cgaggatgat
     5581 gacaaagcag gccgccgcca ggttgaggag gggaacgatg tccgagagag gtaccggggg
     5641 aaatcccggt gccggaaggc ccccgggaac ggggccgagg accgtgatgc cgtagacgga
     5701 taggtcaaag acccagctca cgaggatcgc ccccgcgacc acgatgagag ccccggggat
     5761 ccgtatgctc acccggttcc cgagggcaat caccccaatc acgatgaccg agaggagaag
     5821 ggtgggaata tgggtgagag ggaggtgcag ggcgaagctg actgcctgga gcaccggccc
     5881 tccggagagg ggcgccatcc cgaacatccc accgagctgc ccgagggcga tctggaaccc
     5941 cacgccggtg aggaacccga tgatgaccgc ccgggagagg aaatccgaga caaacccgag
     6001 tttcagcagg cccgcgagga ggaggaatac cgccgacagg agggcgatca tccccgcgta
     6061 tgcgacgtac tggggggact ggggggccgc catcgtcgcg agaccgctcg cgatgatcac
     6121 ggcggtggca gaatcggctc cgaccaccag gtggcgggag gacccgacga gggcgaagac
     6181 cgccatgggg aggaggaggg tgtagaggcc ggtgacaaca ggcatcccgg cgatgttcgc
     6241 atagccgagg gccgcgggga tggcaagggc cgcaaaggtg ataccggcga ggatctcgcc
     6301 cgggacctgg ccggtgtcga ggggcaggat tccctggagg aggtgcaggc ggcggatcca
     6361 ttccggtgcc cggatcgaga ccatgattca cggttctccc ggccgtccaa agcctttccg
     6421 accccgggcg accttcgtct tcccttcgat atgctccccg gtcaacctcg tacctcataa
     6481 gcccgggcga tcacgggaat cattcctgcc tggccctgcc cggaggtgga ggcctgcccc
     6541 tcacgtcttc ctcaccctcg ctgccaggag aagcccgatt ccggcagcaa tgacgccgat
     6601 ggctgcgggg agaggtaccg ccggcgtcgg gctcgtctcc gtcggagtct ccatcggaac
     6661 cggggtaatg agaggaaccg gtgtcggtgt cgcggtcggc accggagttg caggaaccct
     6721 ggttgtcacc ggcatgaagg gggtcttcat ggtagtcggg aggggcagcg ggatcaccgg
     6781 ggccggcgtc gggggtaaac cgaggaccag cccgacgtcc tcgctcaggt ctatcgggtt
     6841 ctcggcggtg atgtggaact gctgcgttgc ggtagccgag gaggtccgga acaggacgac
     6901 cccgtacccg cttgcgtcgg tcctcacctc ggcgtaatag gccgtcgctg aggtcgtgga
     6961 cgaatcggga ggtacatcat cccgtatcgt gcccccgccg ccgatggggt gggacccgag
     7021 ggtgtagggc ccgccgggcg ggtcctgggt gacatccacc tggccgggga cgatgacggg
     7081 cggctggtcg ccgggttccc cggtcatgtc atgggtcccc ttcggccaga tgtcatagag
     7141 ggtcctcgag tgcccgatga ccgtgaccgt gaaagtgttg ccccggacga gggagccgct
     7201 cccctgggca cccgcgatgg agacgaagag gacgagaagg cagatcaatc ctggaaggct
     7261 gaactttcct ggcatacgga ttacctcacg aggttcctgg gtacggcaac ggtacagccg
     7321 ggtctccata aaaacatgtg ggtgaatcgc agaacagtca caaaaggata agtggtcagc
     7381 agccacaggg acccgttgtc caaagaggtg attcccccat ggtcagcaaa aaattttaca
     7441 cactggagga tcttccctat gccttcaatg ccctggagcc tcacatgtcc gaagcccagc
     7501 ttcggttgca ccatgacaag catcacgccg catacgtgaa cggcgcaaac gccatatttg
     7561 aaaggctgga cagggctcgc caggcaggaa cggatatgga catgaaggcc acggtcaagg
     7621 agctgtcctt ccaggcagga ggccatattc tgcacctcct cttctggcag aacctggccc
     7681 ccaccgcgaa ggccgccaag gaacctggcg ggaccctcgc cgaagccctg aagaacgagt
     7741 tcgggtcctt cgagcgcttc aagaaagagt tttcctcggt ggcaaccagt acagagggct
     7801 cgggatgggc ggcgctcgcc aggtgtggcc tcaccggcag gctgctcgtc atgcagatcg
     7861 agaagcataa cgtgaatgtc tacccccacc ttcgcatcct gatggtcctt gacatctggg
     7921 agcatgccta ctacctggac tacaagaacg agaggcccag gtacgtggag gccttctgga
     7981 acatcgtgaa ctgggatgtg gtgaacaatc gactggaggc actcatcaaa taaaagtccc
     8041 ctgacccggt aacggaaacc cctggccgga ttttttctcg ataggggaaa agagggtagt
     8101 ggtggatgtc accggccatt attgtacggt aatctttccc gtcatcgatg ggtgaatgtt
     8161 acagtggaac gggaactggc cggtggaggt aaaggtaaag gagtaggtct ctcctgttcc
     8221 gagaggactg ctccgaaaca actgacccaa tccgtacccg ccaatgggat cattgatgac
     8281 ctggtgcggg gtaggatccc gatttgtcca tgtgaccgtc gtccctttcg aaacggtgaa
     8341 aaatcgtggg gtgaagacaa agttcgtgat ggtgatggac acattggtct ggtttaagat
     8401 agtcgcggtc acctggggcg tgggggtggt cgttgtcacg gttgtggcga gggctgtccc
     8461 cgaaggtgtg ggagcggttg tcgcctgggt cgtagcctgg gtcgtcgccg gcccgtaggg
     8521 tgaccctgtg catccggcca tgaagacggc cagcaggatt gcaacgccga gaaccagaaa
     8581 ccttgttcgt acgatgtcaa ttctcatccg aattcctcat gacatgatag aacctgtggg
     8641 ataaatatga tctccttcga ttgttttcgg gttacagtct attgccaaaa ctcctttgag
     8701 tcgaaaagtg agaggcgcac ataacctaaa ggcagtgtgc accgaccgag actttgtcta
     8761 agtaaacaaa ttcgataaaa atatgctgga agggaagcgt atacaatctt cagggaaagt
     8821 atggcaccga gagcatcagg attcgtactg ttcttcgtgt tcactggacg ataaaccggc
//
LOCUS       DATGDI010000158        10285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_162379, whole genome shotgun sequence.
ACCESSION   DATGDI010000158 DATGDI010000000
VERSION     DATGDI010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10285
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162379"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VKO45_05955"
     CDS             complement(<1..424)
                     /locus_tag="VKO45_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMA05455.1"
                     /translation="MREEEGMQAVILAGGKGRRLLPYTTVLPKPLMPIGEFPILEVIL
                     RQLERAGFSDVVICTGHLHEIIRAYLAQSRHPGLSIRFTHEDSPLGTIGPIRLIEGLR
                     DTFLVMNGDILTDLDYRKLLAAHAGKEAMATIATYHREV"
     gene            complement(421..1410)
                     /locus_tag="VKO45_05960"
     CDS             complement(421..1410)
                     /locus_tag="VKO45_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMA05456.1"
                     /translation="MTWSGKRALVTGAGGFIGSHLVDALVERGAEVTALVRYNSRNDW
                     GMLEERYRSGAGGLRVFPGDVTDAGCVRKAVEGNEVVFHLAALIGIPYSYEAPESYVR
                     TNVLGTLNVLTASLAAGVERVVQTSTSEVYGTAVYTPIDEAHPLQGQSPYSASKIAAD
                     KMAEAFRCSFDLPVVTLRPFNTYGPRQSARAVIPTIITQALAGNIVRLGSLDPVRDFT
                     FVADTVRAFLLAAEKEEAAGKTIHTGSGIGISIGDLAQKILLRVNPGARILTGDERVR
                     PGRSEVMRLVCDSRRAWEILGWRPEVALDEGLSRTIDWIRDYREGYKPGRYTV"
     gene            complement(1407..2495)
                     /locus_tag="VKO45_05965"
     CDS             complement(1407..2495)
                     /locus_tag="VKO45_05965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMA05457.1"
                     /translation="MKLCFIADGRSIHTQRWAEHFGRSHEVHLITYDPSGRSLTGVTE
                     HVIPSFFRNLYLGFWPRHLRILRLIRRIDPDLVHAHFITKYGFHLPLGHRWPTVVSAW
                     GDDILILPPKSLPIRAFTGRVLKKVDLVYAVSQDLRNHIVKDFGIPPGKVIYLPIGID
                     TVVFSPGGKRDTGGRQTVVLFSNRGFYPVYDTRTLLAGFGGALKEKPSLSLVLKGTGP
                     EKGEMQELAASLHIGGAVTFRDRSPYSEVPDDLRSADIFVSTATSDGTPVSVLEAMST
                     GLPCIATRVGGVPEWIEDGENGMLIPPRDPAALTRAILSLASDPALRDRLGARARETV
                     RARGDWGRLMTRVEDDYKRLMSLAGGEK"
     gene            complement(2562..3983)
                     /locus_tag="VKO45_05970"
     CDS             complement(2562..3983)
                     /locus_tag="VKO45_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HMA05458.1"
                     /translation="MEVQRHGLMYLFSTVGITIAGFFATMFYAHWVGAVVLGAYFLFL
                     SYYGILSLFSDSGIGSAGVKRISEGMDQDAYYTACLVLRLLVYLVTIALFILFRDRFA
                     DLNQAGLFSILIIVVGIGIVNAQIGTSIAGCNRLGLAASTSLINNLVRIGIQVVLVFL
                     GYQVAGLVGGLVAGILVQIIIESRFIDLHLSRFTWDHVRSIFTYSTWAFLTSSGNTIF
                     EYVDILVISYFLAVSEVGVYGICWTFSSCAIFVSTALSNTLFVKVSRWDSAGDRGAVG
                     LSLSRAITYSLMFSVPILLGGLFLGGPLLYFLYGADFSAGGIPLLLLIGMRVIQSVQQ
                     IYFTYLMALDHARKAFYVTGTMAVTNLVLDLLLVPAFGIAGAAFASLVTIGTSTVFAH
                     LQLRGFTGITPERRPLFEIAIAAILMTATLMGLLQFITITSAVQAAALVGAGAAIYLG
                     SLTAMDEEIRRSALGILEIRWRS"
     gene            complement(4118..4969)
                     /locus_tag="VKO45_05975"
     CDS             complement(4118..4969)
                     /locus_tag="VKO45_05975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMA05459.1"
                     /translation="MEGDREPVVSVVMPTYNQAGFLREAVRSVLLQDEPRWELLVINN
                     FSEDDTAAVLEGFSDPRIRREDFRNEGIIAASRNRGVGLARGRFVAFLDSDDSWYPGK
                     LSACLGALERGADAVCHGMLIRKDGIPAGNLVPSPPRSLYDELIFRGNTSIATSSVVI
                     RRECLAKAGGFSEDREIVSAEDYELWLRLARGGARFAMLPVILGEYTVHQGGLSRNIG
                     RQSDAEAEVVTREFRRMPDDSLRTILRRRRRMALLHLRAARRFQQAGLYGEAGSALAR
                     SVMALTR"
     gene            complement(4969..5931)
                     /locus_tag="VKO45_05980"
     CDS             complement(4969..5931)
                     /locus_tag="VKO45_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMA05460.1"
                     /translation="MTSEVLITGGLGYLGGRIARALASSSRFSVLVTTRRGECPPLPW
                     LPRDRCRRMDLDDDRQVKEACEGVDAVIHLAALNEVDSAADPRRALRVNGEGTLRLLE
                     AATAAEVGRFIYLSTAHVYGSPLAGRITEATVPRPVHPYAITHRVAEDFVLAARSRGS
                     PEGIVVRLSNGIGAPVLPEVNRWTLVGNDLCRQAVTDHALVLKTPGLQERDFICLSDV
                     ARGVEHLLGLDAGASGDGIFNLGGERSMTILALAGLIRERCGKVLGFTPQLHAPPPGG
                     DPSGKPLDYSIDRLKATGFRLEGRLEDEIDATLAFCREHFGSGS"
     gene            complement(5928..6365)
                     /locus_tag="VKO45_05985"
     CDS             complement(5928..6365)
                     /locus_tag="VKO45_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HMA05461.1"
                     /translation="MKVEPLAQILDERGKVMHMLRRDSPDFLGFGEIYFSCVYPGAVK
                     AWHLHREMTLRYAVPVGEVKLVLFDDRPGSPTKGELQEIFLGPDNYRLVTIPPLIWNG
                     FKGVGPTMALVANCATIPHDPGEIERRDPADPSIPYDWGIHHR"
     gene            complement(6374..7456)
                     /gene="rfbG"
                     /locus_tag="VKO45_05990"
     CDS             complement(6374..7456)
                     /gene="rfbG"
                     /locus_tag="VKO45_05990"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HMA05462.1"
                     /translation="MGDLVGEELRNAFGGERVLVTGSTGFKGSWLACWLHSLGAEVHG
                     FALRPERKEDLFSAAGLESCIRQEYGDIRDYPRLLAFFQAVRPSFVFHLAAQPLVRRS
                     YEEPKLTVDTNVGGSLNVLEAVRNTSGIRSLVYVTSDKCYLNREQREGYREEDVLGGR
                     DLYSASKAAAEIIFAAYSSSFFEGRRDFGAASARAGNVVGGGDWAPDRIVPDCIRAIS
                     EGRPIRLRQPDATRPWQHVLEPLAGYLTLARLLAEDPARFRGSYNFGPDPAGAKTVRE
                     LAEAIVRGWGRGSVEIVGGEKGFGEAGLLYLNSDKARRVLGWRPGWDFRLTIEKTVDW
                     YREYLRGADPLDLTRAQIREYMESFG"
     gene            complement(7441..8220)
                     /gene="rfbF"
                     /locus_tag="VKO45_05995"
     CDS             complement(7441..8220)
                     /gene="rfbF"
                     /locus_tag="VKO45_05995"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HMA05463.1"
                     /translation="MKTVILCGGFGTRIRDVAENIPKPMIPIGGYPILWHIMKHYASG
                     GFDKFVLCLGYKGDVIRDFFFNYRLHTADCTLDLSGKNPARYQGGKVDPWEVTLAETG
                     LTSMTGARVARVRKYVEADGNFMLTYGDGVGDVDLKELLRFHEAHGKILTVTGVRPPG
                     RFGEISCGPDGAVREFNEKPQAAGGWISGGFFVCRREIFRYLDPRDDLVFEQEPLRAL
                     VADGEMMMYRHDGFWMPMDTSREYLLLNSLYAQGKAPWVTW"
     gene            complement(8309..9001)
                     /locus_tag="VKO45_06000"
     CDS             complement(8309..9001)
                     /locus_tag="VKO45_06000"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HMA05464.1"
                     /translation="MLIPLSYPLTLNSPLYPGTPVPAVRPYNPPGSGTHPRSSIISVH
                     SHSGTHIDLPPHFCPEGIGNDPLSPPGLTFFPARCIGIPLDPASTITPGLLIPHLPSI
                     RDAAAILVRTGSGAGRSTDPEGYASSHPRVDPSVPAWLRAESPGLRLFGIDAISIADP
                     SHRDEGRACHREFLCGSPPILILEDADLEDDRILDDPWTLHLFPLLAEWIDGLPVVAI
                     ADTGDALWNRYI"
     gene            complement(8995..9780)
                     /locus_tag="VKO45_06005"
     CDS             complement(8995..9780)
                     /locus_tag="VKO45_06005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HMA05465.1"
                     /translation="MAVANRGFLRVIRFRFREYRACHPMRMIMLEVGRIYNEQAVQPP
                     CYQLIILDFDGVILESVSIKTDAFRELFRPYPEHLDRILAFHLGNLGTSRFDKIRYIY
                     RDILKKDLSPDEYQRLLARYADLVLEKVLRAPFVSGALEFLERFHRKVPLYIVSATPQ
                     DEIRFIVQERGLSRYFHGVYGSPMKKVESMREILATSGTEPGSALFVGDAQNDLHAAR
                     ALGVPFVGRVSRGAANSFLGESDVVGVVNDLDELGKYIGEREC"
     gene            9746..>10285
                     /locus_tag="VKO45_06010"
     CDS             9746..>10285
                     /locus_tag="VKO45_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMA05466.1"
                     /translation="MTRRNPRFATAITIRSFDTGGAAMRALEEASEVVFVNRTGGHLT
                     GPGLEGALSGADAVIAGTEAFTPAVLRSASRLKVISRVGVGTDNIDPSASRGIRICTT
                     PRAPIQAVAEHTVALLLAIAKRVVTYNGNIRRGDFSVSPGILLAGKRVGLIGAGRIGR
                     RVGELLEAFGCIVLFHDPAD"
BASE COUNT         1920 a         3211 c         3370 g         1784 t
ORIGIN      
        1 tcacctcccg gtgatacgtc gcgatggtgg ccatggcctc cttccctgcg tgggccgcga
       61 ggagtttccg gtagtccagg tcggtgagga tgtccccgtt catgacgagg aaggtatccc
      121 ggaggccctc gatgagcctg atagggccga tggttcccag gggcgaatcc tcgtgggtaa
      181 accggatcga gagaccgggg tgcctgctct gggcgagata ggcgcggatg atctcgtgga
      241 ggtgcccggt gcagatgaca acgtcggaga aaccggcccg ttcgagctgg cgcaggatca
      301 cctcgaggat gggaaactcc ccgatgggca tcaggggctt ggggaggacc gtggtatagg
      361 ggagaagcct cctccccttc cctcccgcga ggatgacggc ctgcattccc tcttcctccc
      421 tcataccgtg tacctccccg gcttgtagcc ctcccggtaa tcccggatcc agtcgatggt
      481 ccgggagagc ccctcgtcca gggccacctc cggtcgccag ccaaggatct cccaggcgcg
      541 gcggctgtcg cagacgaggc gcatcacctc gctcctgccg ggccggaccc gctcgtcccc
      601 ggtaaggatc cgggcgcccg ggttcacccg gaggaggatc ttctgcgcga ggtcgccgat
      661 ggagatcccg atgcccgacc cggtgtggat ggtctttcct gcagcctcct ccttctcggc
      721 ggcgaggaga aaggcacgca cggtgtcggc gacaaaggtg aagtcccgga cgggatcgag
      781 gctccccagc ctgacgatgt ttccggcgag ggcctgggtg atgatggtcg gaatgaccgc
      841 ccgggcggac tgccggggcc cgtaggtgtt gaagggccgg agagtgacga cgggaagatc
      901 gaaggagcag cgaaaggcct cggccatctt gtcggcggcg atcttggagg ccgagtaggg
      961 agactgtccc tggaggggat gggcctcgtc gatgggggtg tagacggcgg tcccgtacac
     1021 ctcagacgtg gaggtctgga ccaccctctc cacccccgcc gcgagggatg cggtgaggac
     1081 gttcagcgtc ccgaggacgt ttgtcctcac ataggactcc ggtgcctcgt aggagtaagg
     1141 gatcccgatg agggccgcga ggtggaagac cacctcgttt ccctcgacgg cctttctgac
     1201 gcatcccgca tcggtcacgt cccccgggaa cacccggagg cccccggctc cgctccggta
     1261 cctctcctcg agcatccccc agtcgttccg ggaattgtac ctgacgaggg cggtgacctc
     1321 ggcccccctc tccacgaggg catccaccag gtggctccct atgaaacccc ccgcacccgt
     1381 gacaagcgct cgttttccgc tccaggtcat ttctcccctc cggcgaggct catcagcctt
     1441 ttatagtcat cttccacccg cgtcatcaac cttccccagt cccccctcgc cctgaccgtt
     1501 tccctggccc gcgctcccag ccggtcccgg agggccgggt ccgaggcgag ggagaggatg
     1561 gcccgggtga gggccgcggg gtcccgggga gggatgagca tcccgttctc cccgtcctcg
     1621 atccattcgg ggaccccccc gacccgggtc gcgatgcagg ggaggcccgt ggacatggcc
     1681 tcgaggaccg agaccggcgt cccgtccgac gttgcggtgg agacgaagat gtctgcactc
     1741 ctgagatcat ccggaacctc ggagtagggg gaacgatccc tgaaggtaac ggcaccgccg
     1801 atgtgcaagg acgcggcgag ctcctgcatc tcccccttct cgggtccggt gcccttcagg
     1861 acgagggaga gggagggttt ctccttcagg gcaccgccaa agcctgcgag gagtgtgcgg
     1921 gtgtcgtaca ccgggtagaa tccccggttg gagaagagga ccacggtctg acgcccacct
     1981 gtatcccgct tcccccccgg agaaaatacc accgtgtcta tcccgatggg caggtagatc
     2041 accttcccgg gagggatccc gaagtccttc acgatgtggt tcctgaggtc ctgcgagacc
     2101 gcatagacca ggtccacctt cttgaggacc cttcccgtga aggcacggat cgggagggat
     2161 ttcgggggaa ggatcaggat atcatccccc catgccgaga ccacggtcgg ccaccggtgc
     2221 cccaggggga ggtgaaaccc gtacttcgtg atgaagtggg cgtgcacaag atctgggtcg
     2281 atccttcgga tgaggcgcag gatgcggagg tgccggggcc agaacccgag gtagaggttc
     2341 cgaaaaaaag acgggatgac atgttcggtg accccggtaa gggaccggcc cgaggggtcg
     2401 taggtgatca ggtgcacctc gtggctcctt ccgaagtgct ccgcccaccg ctgggtatgg
     2461 atggatcggc cgtcggcgat gaagcagagt ttcatcccgt caccctcacc tgcccctgaa
     2521 ccatgtccgg atcatcattt cagtttcctc caggtgcttt tttacgatct ccatctgatc
     2581 tcaaggattc ccagggcaga tctccgaatc tcttcatcca tggctgtcag ggacccgagg
     2641 tagatggccg caccggctcc cacgagagcc gcggcctgta ccgcgctcgt tatggtgatg
     2701 aactgcagga gccccatgag ggtggcggtc atgaggatgg cggcgatggc gatttcgaag
     2761 agcggccttc tctcgggggt aatcccggtg aaccccctca gctggaggtg ggcgaagacg
     2821 gtgctcgtcc cgatggtgac gagggatgcg aaggcggctc cggcaatccc gaaggcgggg
     2881 acgagaagga gatcgagcac gagattcgtg acagccatgg ttccggtgac gtagaaggcc
     2941 ttccgggcat ggtcgagtgc catgaggtag gtgaaataga tctgctggac tgactggatg
     3001 acccgcatcc cgatgaggag gaggagggga atgccgcccg ccgagaaatc ggccccgtag
     3061 aggaagtaga ggaggggacc cccgaggaac aggcccccga ggaggatggg gaccgagaac
     3121 atcagggagt aggtgatggc ccgggagagg gaaaggccga ccgcgccccg gtccccggcc
     3181 gagtcccagc ggctcacctt cacaaagagg gtgtttgaga gggccgtgct cacgaagatg
     3241 gcacacgacg agaaggtcca gcagatccca tagacgccca cctccgaaac ggcgagaaag
     3301 tagctgatga ccaggatatc cacgtactcg aagatggtat tccccgatga cgtgagaaaa
     3361 gcccaggttg agtaggtgaa aatactcctg acatggtccc aggtgaaccg actgagatgg
     3421 agatcgatga accgggactc gatgatgatc tggacgagga tcccggcgac gagtccaccg
     3481 accaggccgg caacctggta ccccaggaac acgaggacca cctggatgcc gatccggacg
     3541 aggttgttga tgaggctcgt cgaggcggcc agcccgagac ggttgcatcc ggcgatggag
     3601 gtcccgatct gggcattgac gataccgatt cccaccacga tgatgaggat agagaataac
     3661 ccggcctggt tcaggtcggc gaaccggtcc cggaacagga tgaagagggc gatggtgacg
     3721 aggtagacca ggagccggag cacgaggcac gcggtatagt aggcatcctg atccatgccc
     3781 tcgctgatcc tcttcactcc cgcggatccg atccccgaat ccgagaagag agagaggatg
     3841 ccgtagtagg agaggaacag gaagtaggcg cccaggacca cggcccccac ccagtgggca
     3901 tagaacatcg tggcaaagaa tcccgcgatg gtaatcccca cggtggagaa gaggtacatc
     3961 aacccgtgcc gctgcacctc catctcccgt gactcggtct gatctggcgt ggattgcccc
     4021 ccggggctca tgtcctgggc cttccgaagc tttactacag ctctccccga gaattgataa
     4081 tcccttggtt acccggctga gagcgggaca gaggcgtcta tcgggtcagg gccatgaccg
     4141 atctggccag tgccgagccg gcttctccgt acaatcctgc ctgctggaac cttctcgctg
     4201 cccggagatg gagaagggcc attctccgcc tcctgcgcag gatggtgcgg agggagtcat
     4261 ccggcatccg gcggaattcc cgggtgacga cctctgcctc ggcatccgac tgcctgccga
     4321 tattccggct gagtcctccc tggtggaccg tgtattcccc gagaatgacc gggagcatgg
     4381 cgaaccgggc acccccccgt gcaagtctga gccacagctc gtaatcctcg gcggaaacga
     4441 tctcccggtc ctcggaaaag ccccctgcct tcgcgaggca ttccctccgg atcaccaccg
     4501 aggaggtcgc gatggaggta ttgcccctga agatgagctc atcgtacagg cttcggggtg
     4561 gcgatgggac gagattcccg gcggggatac cgtccttccg gatcagcatc ccgtggcaga
     4621 cggcatcggc acccctttcc agggcaccca ggcaggccga cagttttccc gggtaccagg
     4681 aatcgtcgga atcgaggaat gcgacgaacc ggccgcgggc gaggcccact ccccggttcc
     4741 gggatgccgc gatgataccc tcgttccgga aatcctcgcg ccggatgcga ggatcggaga
     4801 agccctccag caccgcggcg gtgtcgtcct ccgagaaatt gttgatgacc aggagctccc
     4861 agcggggctc gtcctggagg aggaccgatc tcaccgcctc cctgagaaaa cccgcctggt
     4921 tataggtggg catcaccacc gatacgacgg gttcccgatc cccttccatc acgatcccga
     4981 cccgaagtgc tcacggcaga aggcaagggt cgcgtcgatc tcgtcctcca gccttccctc
     5041 gaggcgaaag cccgtcgcct tcagcctgtc gatggagtaa tcgagaggct tcccggaggg
     5101 gtcgcccccg gggggaggtg cgtggagttg aggggtgaac ccgaggacct tcccgcatcg
     5161 ctcccggatg agcccggcga gggcaaggat ggtcatgctc ctctcccctc ccaggttgaa
     5221 gattccgtcg ccggaggctc cggcatccag gcccagcagg tgctccaccc cgcgggcgac
     5281 gtcggagagg cagatgaagt cccgctcctg gagcccgggg gtcttcagca cgagggcatg
     5341 gtccgtgacc gcctggcggc agaggtcgtt cccgaccagg gtccaccggt tcacctccgg
     5401 gaggacgggg gcgccgatcc cgttggacag gcggaccacg atcccctcgg gagacccccg
     5461 ggatcgggcg gcaaggacga aatcctccgc gacccggtgg gtgatggcgt acgggtggac
     5521 aggtctcggg accgtagcct cggtgatcct tccggcgagg ggagacccgt acacgtgggc
     5581 cgtggagaga tagatgaaac ggcccacctc cgccgcggtg gccgcctcga ggagcctcag
     5641 ggtgccctcc ccgttgaccc ggagcgccct tcgggggtcg gcggccgagt caacctcgtt
     5701 taaggccgcg agatggataa cggcgtccac accctcgcag gcctccttca cctggcggtc
     5761 gtcatcaagg tccatccgcc tgcaccgatc ccggggaagc cagggaagag gggggcactc
     5821 tccccgccgg gtggtgacca ggaccgagaa ccgggacgag gatgccaggg cccgggcgat
     5881 ccttcccccc aggtacccga gccctccggt gatcaggact tcactcgtca tcggtggtgg
     5941 ataccccagt cgtacgggat ggagggatcc gcggggtccc gtcgctcaat ctccccggga
     6001 tcatggggaa tggtggcgca gttggccacg agggccatgg tggggccgac ccccttgaac
     6061 ccgttccaga tcaggggagg tatcgtcacc aggcggtagt tgtccggccc gaggaagatc
     6121 tcctggagct ctcccttcgt cgggctcccg ggccggtcgt cgaagagcac cagcttgacc
     6181 tcgcccaccg ggaccgcgta ccggagggtc atctcccggt ggaggtgcca ggccttgacg
     6241 gcccccggat agacgcacga gaagtagatc tccccgaaac cgaggaaatc cggcgaatcc
     6301 cgcctgagca tgtgcatgac cttgccccgc tcgtccagga tctgggcgag gggctcgacc
     6361 ttcactcccg aaatcatccg aaactctcca tgtattcccg tatctgggcc ctcgtgagat
     6421 ccagggggtc cgcccccctc aggtactccc ggtaccaatc cacggtcttt tcaatggtca
     6481 aacggaaatc ccatcccggc ctccatccga gcacccgccg ggccttgtct gagttcaggt
     6541 acaggaggcc ggcctcgccg aatcccttct cgcccccgac aatttcgacg gacccccggc
     6601 cccatccccg gacgatggcc tctgcgagct ctctcacggt cttcgcccct gccgggtcgg
     6661 gcccgaagtt gtaggatccc cggaaccggg cggggtcctc tgcgaggagc cgggcaaggg
     6721 tcaggtaccc ggcgaggggc tccaggacgt gctgccacgg cctcgtggca tcgggctggc
     6781 gcagccggat cggccttccc tccgagatgg cccggatgca gtccgggacg atccggtccg
     6841 gggcccagtc accgccgccc acgacgttcc ccgcccgggc actcgcagcc ccgaaatccc
     6901 gccggccctc gaagaaggac gatgagtagg ccgcgaagat gatctccgcc gccgccttgg
     6961 aggcgctgta caggtcccga ccccccagga cgtcctcttc ccggtaaccc tcccgctgtt
     7021 cgcggttcag gtagcacttg tccgaggtca cgtagacgag agaccggatc cctgaagtgt
     7081 tccggacggc ctcgaggacg ttcagggatc ccccgacgtt cgtgtccacg gtgagcttcg
     7141 gctcctcgta ggagcgccgg acgaggggct gggccgcgag gtgaaagaca aaggaaggcc
     7201 tgaccgcctg gaaaaaagca aggagccggg ggtaatccct gatatccccg tactcctgcc
     7261 tgatgcagct ctcgagcccg gcggcggaga agaggtcctc cttcctctcg ggccggaggg
     7321 caaatccgtg gacctcggcc ccgagggagt ggagccagca ggcgagccag gaacccttga
     7381 acccggtgct ccccgtgacg aggacccgct ctcccccgaa ggcgttccgg agttcctccc
     7441 ctaccaggtc acccatggcg ccttcccctg ggcatagagg gaattcagga ggaggtactc
     7501 ccgggaggtg tccatcggca tccagaaacc gtcgtgccgg tacatcatca tctccccgtc
     7561 ggcgacgaga gcccggaggg gctcctgctc gaagaccagg tcatcgcggg gatcgaggta
     7621 tcggaagatc tccctgcggc acacgaagaa gccgccggat atccatcccc cggccgcctg
     7681 gggtttttcg ttgaactccc ggacggcccc gtcggggccg caggagatct cgccgaaccg
     7741 gccggggggc cggaccccgg tcaccgtgag gatcttcccg tgggcctcgt ggaacctgag
     7801 aagctccttc aggtccacgt cccccacccc gtcgccgtag gtgagcatga agttcccatc
     7861 cgcctccacg tatttccgga cccgggccac ccgggccccg gtcatgctcg tgagtcccgt
     7921 ctctgcgagg gtcacctccc aggggtcgac cttcccgcct tggtacctcg ccgggttctt
     7981 ccccgagaga tccagggtac aatcggcggt gtgcagccgg tagttgaaga agaagtcccg
     8041 gatcacatcc cccttgtacc cgaggcagag aacgaatttg tcgaagccac ccgaggcata
     8101 gtgcttcatg atgtgccaga ggatggggta cccgccgatg gggatcatcg gcttggggat
     8161 gttctcggcc acatcccgga tgcgggttcc aaacccgccg cagagaatca cggtcttcat
     8221 cgggagcact ccctcgatgg gcctggtatc ggctgtacaa gatggatccc ggaactgtaa
     8281 agattttccg gaaaagggat acaaatcttc agatgtaccg gttccaaagt gcatcaccgg
     8341 tatccgcgat ggccacgacg gggagcccgt cgatccattc tgccaggagg ggaaataggt
     8401 ggagcgtcca gggatcatcg aggatacggt catcttccag gtcggcgtcc tccaggatga
     8461 ggatgggagg ggagccgcag aggaactccc ggtggcaggc cctcccctca tcccggtggg
     8521 aagggtccgc gatggagatg gcatcgatcc cgaagagcct gaggcccggg ctctcggctc
     8581 ggagccaggc ggggacggag ggatccactc tcgggtgaga ggaggcgtat ccttccgggt
     8641 cggtggatct cccggcgccg gagccggtcc ggacgaggat agccgcggca tcccggatcg
     8701 agggaagatg ggggatgagg agcccaggcg tgatggtcga agcgggatcc agcggtattc
     8761 ctatgcaccg tgccgggaag aaggtaagac ctggtggtga aaggggatcg ttcccgattc
     8821 cctcggggca gaaatgggga gggaggtcga tgtgggtccc cgagtgggag tggaccgaga
     8881 tgatgctgga ccgggggtgg gtaccggagc ccgggggatt gtagggtctt accgcgggaa
     8941 cgggggtacc cggatagagg ggggaattca gggtgagggg atacgacaga gggatcagca
     9001 ttctcgttcc ccgatatact tcccgagttc atcgagatcg tttacgactc ccacgacatc
     9061 actttcccca aggaaggagt ttgctgcacc tctgctcacc ctccccacga agggcacacc
     9121 cagggccctt gccgcgtgca gatcgttctg agcatcgcca acgaagaggg cactccccgg
     9181 ttcggtccct gaagttgcga ggatctccct catggattcc accttcttca taggtgagcc
     9241 gtaaacgcca tggaaatacc gggaaaggcc cctctcctga acaatgaacc tgatctcgtc
     9301 ctgcggggtt gccgatacga tatagagagg aaccttcctg tggaaccgct ccaggaattc
     9361 cagggcgccg gagacgaagg gggctcggag gaccttttca aggacgagat cggcataccg
     9421 ggcgaggagc ctttgatatt catcgggaga gaggtccttt ttgagaatat cacggtaaat
     9481 gtaccggatc ttgtcgaacc tcgaggtgcc gagattcccg agatggaatg cgaggatgcg
     9541 atcgagatgc tctggatacg gtctgaagag ttcgcggaat gcatctgtct ttatcgatac
     9601 cgattcgagg atcacaccat caaagtccag gatgataagc tggtaacacg ggggttgcac
     9661 ggcctgttca ttatatattc tcccgacctc caacataatc atgcgcatcg ggtgacaggc
     9721 ccgatattcc ctgaaacgga atcttatgac ccggaggaac ccgcggtttg ccaccgccat
     9781 caccatccgt tcctttgata ccggtggtgc cgccatgcgg gcgctcgagg aggcaagcga
     9841 ggtggtcttc gtgaaccgga ccggaggcca tctcaccggg ccgggcctgg aaggggccct
     9901 ttccggcgcc gacgcggtga tcgccggcac cgaggccttt acccccgccg tgttgagatc
     9961 cgcctcccgc ctgaaggtca tctcgcgggt gggggtgggt acggataata tcgacccctc
    10021 tgcgtcgcgg gggatccgga tatgtacgac cccccgggcc cccatccagg cggtggcaga
    10081 gcatacggtg gccctcctcc ttgcgatcgc caagagggtg gtgacgtaca atgggaatat
    10141 ccggcgggga gacttctctg tctccccggg tattctcctc gcagggaagc gtgtcggtct
    10201 cataggcgcc gggcggatag ggagaagggt gggagagctt ctcgaggcct tcggatgtat
    10261 tgtcctgttc catgatcccg cggac
//
LOCUS       DATGDI010000159         9966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_365802, whole genome shotgun sequence.
ACCESSION   DATGDI010000159 DATGDI010000000
VERSION     DATGDI010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 9966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9966
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365802"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(832..1050)
                     /locus_tag="VKO45_06015"
     CDS             complement(832..1050)
                     /locus_tag="VKO45_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMA05467.1"
                     /translation="MRNKIKVYRAMHDLTQEDLARAIGVTRQTILAIEKGKYVPSLDL
                     AFRIARHFHVNVEEVFLYGDEFLPPAAG"
     gene            complement(1040..1486)
                     /locus_tag="VKO45_06020"
     CDS             complement(1040..1486)
                     /locus_tag="VKO45_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2178 domain-containing protein"
                     /protein_id="HMA05468.1"
                     /translation="MKKNTFYLLIGGIALLLLTILWYSVEIHRPLLIEIAFIAAIAVV
                     YLARRRVEDLAEDERSVKITEQAVVRTFQVFWVVFAAFSIGAVMEILYVPSFPRPSLP
                     ERPREILPPRLMGYIQLGLLILMIFLYVGFRIYYARKYGEWETDEE"
     gene            1721..2986
                     /locus_tag="VKO45_06025"
     CDS             1721..2986
                     /locus_tag="VKO45_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05469.1"
                     /translation="MKPHAYLILLLLLVAAAGGAASAQATSDTAFSQIVVSKITINPG
                     VFMTGDVGTITITVLNTGTEAVPIARATFFTDGVVLLNPGAYDQVMTLGPDNSRDFTF
                     NVQATGTGGLYQPRFYLDFQGSGSMSSYVPLQIDNTGLQVSVDSLPDYFATGRTDEIA
                     LRVGNPRSNNLTSITIIPSGAGVRTLESNYFVGDLGAGNSRDVTFTVIPSTQTELTFH
                     TTYRNGINAHSTDTVVPILIDNSRRSADLVINNVKITPSTGSYQLDADITNGGLDDAK
                     SVVVTVQSPAQPVDPYPSYVIGTLAVDDFSSFSVTYSAPATLSTLPVLVQWKDSDGNS
                     YQNTVNINLRSAAAGGSTGSQTGTITRTGGGGPFGFFGGGRGLAIPVIPIIVIIVIAV
                     VLFFAWRRGIFNRFLKKGKGKQGNQQIRQ"
     gene            3002..3676
                     /locus_tag="VKO45_06030"
     CDS             3002..3676
                     /locus_tag="VKO45_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA05470.1"
                     /translation="MSGDPVISLLDVTKVYHMPAEDVVALDHVSLDIARGDFIAIMGP
                     SGSGKSTLLNQIGCLDRPTSGEVLINGRAIRDMNDNELTDLRRDTIGYIFQTFNLIAL
                     LTAYENVEYPLIIRHKTRAGREEILNLLREVGIDEALANHIPGELSGGQQQRVAIARA
                     LVNNPDILLCDEPTGNLDSKTGTQIMEMLRSFNEKGKTIIMVTHDTRIAGYAHRTINI
                     RDGRMA"
     gene            3673..4884
                     /locus_tag="VKO45_06035"
     CDS             3673..4884
                     /locus_tag="VKO45_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA05471.1"
                     /translation="MIGGDIFTSLAYRSVRRHLLRSLLAALGIVIGVVAITTLGIMGA
                     NLTLSVTAQLSSSGNVIIITPYTGGGRGFVGPGGGGEGTNLNITQSQLQEIERVVGAN
                     TVIPIYSKSDQITVGSKQGRASILGLKPDTVQNILVVTDGTFLRGGDGALVGPTLAND
                     FSLKVGSRIKLGSSSDNSTRNVRVVGILQSTGFGAGLSTDRAIVVTDKWYTAAYGGSG
                     EYSQVNVIVGNINQIGTIENNTDKALNRNQKLPAVRITDSGQFLTTITQALGSITSFV
                     TMLGAISLVVAAVSIFNVMIMSVSERVREIGILRSIGTRKSEIRKMFLYEAVIIGLIG
                     AVIGALMSVVSSYLVVTAIVGNTTYFFTPASLAYIPQGMLVGVATCVLSGVYPAWRAA
                     NLDPIEALRAE"
     gene            complement(5026..6252)
                     /gene="thiC"
                     /locus_tag="VKO45_06040"
     CDS             complement(5026..6252)
                     /gene="thiC"
                     /locus_tag="VKO45_06040"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HMA05472.1"
                     /translation="MKTLIDHAREGRVTGPVRTVAGSEMRDPSLILDGIAGGSVTIMR
                     RGKRCLGIGHGLRTKVNVNIGTSPLRIDPEAEVEKAVIAEKYGADTLTDLSMGGDIRD
                     IRRKIFRATKLPLTTVPIYEAAARIGIGTMTTKEILRILREQADEGVSSVVLHCTSRD
                     LLDTLRGGKRIMGVVSKGGSITCAWMMRTGSENPFLEHFDEVLSILKRRDIVLSLGNT
                     MRSGCIHDPMDRAQKGEMMLNASLARRAHDAGVQTIIEGIGGHVRADRIAAYIRRYRR
                     LSEAPLFVAGPLPTDIAVGRDHMAGCVGASIAAGAGADYLCYITPAEHLGLPDPGQVK
                     EGLMAFRIAAHIGDSIKYGPDARDRELSRRRADLDWEGQFSRALDGETARALAPADGP
                     CTMCGEFCAIQMMRDL"
     gene            complement(6252..6794)
                     /locus_tag="VKO45_06045"
     CDS             complement(6252..6794)
                     /locus_tag="VKO45_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05473.1"
                     /translation="MIVDTALKDGSFENFPLRIVASTLLVQAAIYILGAYIVLQFGLV
                     FLAIYIACVLGLEYRVLRHSCVNCSYYGMTCCFGRGRLCALIFRKGDPKRFRERKIGW
                     VDILPDFLISTIPLLLGIILLVLRFDVLLLAVVVALALLAFPGTGFIRSRWACRYCRQ
                     RQVGCPAEHLFGRKGPEEAR"
     gene            6912..7370
                     /locus_tag="VKO45_06050"
     CDS             6912..7370
                     /locus_tag="VKO45_06050"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HMA05474.1"
                     /translation="MGSLEENIGYHFRDPALLTRALTRKAFANEEMHQGRQHEDQEIL
                     STLGDAALKAAFVDLLVRSHYPTPDAITREKESLERHDALAGIALQIGIGPSIRLGSG
                     EKKREAEQEPYVLAETLEAIIGAIYLDGGCEAARNAVVRWYGHRIRPHPG"
     gene            7604..7867
                     /locus_tag="VKO45_06055"
     CDS             7604..7867
                     /locus_tag="VKO45_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05475.1"
                     /translation="MTGMLPQTKAFVPDSAQIMILREVSDCPSCSITHVVEKLLPSHS
                     ESIVRSRVRELLARRYLEWGKSPSAIQLRLTSQGRVALQREPV"
     gene            complement(8000..8084)
                     /locus_tag="VKO45_06060"
     tRNA            complement(8000..8084)
                     /locus_tag="VKO45_06060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8046..8048),aa:Leu,seq:cag)
     gene            complement(8516..9571)
                     /locus_tag="VKO45_06065"
     CDS             complement(8516..9571)
                     /locus_tag="VKO45_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HMA05476.1"
                     /translation="MTGSSFSKGPVYAVAAAVLFGASTPLAKILLGEIDPILFAALLY
                     LGSGTGVLLLGRFRRPDGKAPRELRLDARDYPWLTGAILTGGVLAPISLLYGLRATSA
                     ATASLLLNFELVATTLIAFFIFAEFIGRRVLAAIAVITVAGIILTWNMTGLPGFSPAS
                     LGIVAACALWGIDNNLTRKISGKDPLSIAVVKGMGAGGVSFLIAMLTRAAVPGFQVVA
                     AALIVGFVTYGLSIVFFVRALRDLGATRTSAYFASAPFIGSIASLLLLREVPGTQFLI
                     ALPLFLLGVALLVSERHIPGHFHERIVHAHRHRHDELHDHPHPGEDTGEHVHEHVHEP
                     VDHENPPAPDIHHGHER"
BASE COUNT         2037 a         3049 c         3049 g         1831 t
ORIGIN      
        1 tttctccccc gtatcttcct ggccacgaga tcggggagct ctgaggcgag aaaagttcgc
       61 ttctcttcag ccaggggccg cagggccgca aggatctccc ccccgctcat cccggtaggg
      121 taccgtcgtg gatgagataa aaccctccgc accgccggac ctgttccacg agctcccgat
      181 tgccggaaac caggtttttc agggtggggg agggcgggtc ccgtcagaat ctcatcgccc
      241 cgccggcgac ctgtcaaccg gtcctcggcg gatgggaatt ctaaccataa aggtttcccg
      301 gagaagaaac ccgatttctg ggattcgccg gatattccaa cagaaaggga tgcatatttt
      361 gggcatctat ttcaacattt ctctttccca tcgttaacgt ttttcgttat attattataa
      421 aatatatccc ctccaataaa ttaaaatagt taaccgtgta gagatactct acagggtccg
      481 gatcagcagt ggtccctcgg cggaattgga tccggccctg atctttcggg gaagatgcca
      541 ttggtcgcga tgcatggtag cctgtcccag aatagaaggc tctcagtgga aaatcccaaa
      601 cccccgggat ttgcacggct gcattcttcg gtaatggaac ccacaggcac gaatcttttc
      661 agggctccag ttcccccact ccccgatgac cggggataga accggtgcgg tggcaggcga
      721 gaattccatg cctcggggat ctccccgatt ttcgaaggct tccggtgcct atcgtatctc
      781 cgcgatacga tgaccgggtg cgaaggagag gccggtaccg ggaaggaaag atcaccctgc
      841 cgccggcggg aggaactcat ccccgtagag gaagacctcc tcgacgttga catggaagtg
      901 gcgggcaatc cggaaggcga gatccaggga agggacgtac ttccccttct cgatggcgag
      961 gatggtctgc ctcgtgaccc cgatggccct cgcgagatcc tcctgggtca ggtcatgcat
     1021 cgcccggtag accttgatct tattcctcat cggtctccca ctccccgtac ttccgggcgt
     1081 aatagatcct gaacccgacg tagaggaaga tcatgaggat caacaggccg agctggatgt
     1141 atcccatcag cctaggggga aggatctcgc gggggcgctc cgggagggag ggcctcggga
     1201 acgaggggac atagaggatc tccatgaccg ccccgatgga gaaggcggcg aagaccaccc
     1261 agaagacctg gaaggtccgc acgacggcct gctcggtgat cttgacgctc cgttcatcct
     1321 cggcgagatc ctcgacccgc cgccgcgcga ggtacacgac ggctatggcg gcgatgaagg
     1381 cgatctcgat gagaagcggc cggtggatct ccaccgagta ccagaggatg gtgaggagga
     1441 ggagggcgat gcccccgatg aggaggtaga aggtgttctt cttcatggcc ggttatccca
     1501 tcttctgtta aatatatttt acatatggtg tatatgaccg tttagatgcc ggcggggata
     1561 ccggtcgggt gaggaatgcc cgcccggcgg aaatgccgtg cggaatcagt aaccattaaa
     1621 taactaaatg taaaatatat attacaattt cagggatcat gtttatggac atccatgctg
     1681 aaggaaaaag acgcgagcga gacatcaacc ggtgaaccag atgaagcctc acgcatacct
     1741 gatcctcctc ctcctccttg tcgcggccgc cggaggcgcc gcctctgccc aggccacgtc
     1801 ggacacggcc ttctcccaga tcgtggtctc aaagatcacg atcaatccgg gcgtgttcat
     1861 gaccggggat gtcgggacca tcaccatcac cgtcctgaac accgggacag aggcggttcc
     1921 catcgcccgg gccacctttt tcacggacgg ggtcgtcctc ctgaacccgg gcgcctatga
     1981 ccaggtcatg accctggggc ccgacaactc ccgggatttc accttcaatg tccaggcgac
     2041 ggggaccggg gggctgtacc agcccaggtt ttacctcgat ttccagggat ccgggagcat
     2101 gagctcgtac gtgccgctcc agatcgataa caccggcctc caggtctcgg tggacagcct
     2161 ccccgattat ttcgccacgg ggagaacgga cgagatcgcc ctccgggtcg ggaatccccg
     2221 ttccaataac ctgacgagca tcaccatcat cccctcgggg gcgggggtca ggaccctgga
     2281 gagtaactac ttcgtcgggg acctcggtgc cgggaattcc agggatgtga cctttaccgt
     2341 tatcccctcg acccagacgg agctcacctt ccatacgacc taccggaacg ggatcaatgc
     2401 ccactcgacg gacacggtgg ttcccatcct gatcgacaac agcagacgga gcgcggatct
     2461 cgtcatcaac aacgtgaaga tcacaccctc gacggggagc taccagctcg acgcggatat
     2521 cacgaacggg gggctcgatg acgcgaagtc cgtcgtggtc acggtgcaga gtccggcaca
     2581 gccggtggac ccgtacccct cttatgtcat cgggaccctt gccgtggatg acttctccag
     2641 cttctcggtg acctattcgg caccggcgac cctgagcacg ctcccggtcc tcgtccagtg
     2701 gaaggattcc gacggtaatt cgtaccagaa caccgtgaac atcaacctcc gcagcgcggc
     2761 ggcgggcgga agcacgggtt cccagacggg caccatcacc cggacggggg gcggaggccc
     2821 cttcggcttc ttcggtgggg gccgcggcct tgcgatcccc gtcatcccta tcatcgtcat
     2881 catcgtcatt gcggtcgtcc tgttcttcgc atggcggagg gggatcttca accggttcct
     2941 gaagaaggga aaaggaaagc agggaaatca gcagatcagg caatgagcgg gacaggcgat
     3001 catgagcggt gacccggtca tcagcctcct cgacgtgacg aaggtctacc acatgcccgc
     3061 cgaggacgtg gtggccctcg accacgtctc cctcgatatc gcaagggggg atttcatcgc
     3121 catcatgggc ccgtccgggt ccgggaagtc caccctcctc aaccagatcg gctgcctcga
     3181 caggccgacc tccggggagg tcctcatcaa cgggagggcc atccgggata tgaacgataa
     3241 cgagctcacc gacctccggc gggacaccat cgggtacatc ttccagacct tcaacctcat
     3301 tgccctcctc accgcctacg agaatgtgga gtatcccctg attatccggc acaagacccg
     3361 cgccgggagg gaggagatcc tgaacctcct ccgggaggtg gggatcgacg aggccctggc
     3421 gaaccatatc ccgggcgaac tctccggggg gcagcagcaa agggtggcca ttgcacgggc
     3481 cctcgtgaac aatcccgata tcctcctctg cgacgaaccc acggggaacc tggactcaaa
     3541 gacaggcacc cagatcatgg agatgctccg cagcttcaac gagaagggga agaccatcat
     3601 catggtcacc catgacacga ggattgcggg atacgcccac cggaccatca acatccggga
     3661 cgggagaatg gcatgatcgg cggggatatc tttacctccc tcgcctaccg gagcgtcagg
     3721 cgccacctcc tccggtcgct cctcgcggcc ctcggcatcg tcatcggggt ggttgccatc
     3781 acgaccctgg ggatcatggg cgcaaacctg accctctcgg tgaccgccca gctctccagt
     3841 tcggggaacg tgatcatcat caccccgtac acgggcgggg ggagaggctt tgtcggtcca
     3901 ggcgggggag gtgaggggac aaacctgaac atcacccaga gccagctcca ggagatcgag
     3961 agggtcgtgg gggccaacac ggtgatcccc atctactcca agtcggacca gatcacggtg
     4021 ggatccaaac aggggagggc gagcatcctc gggttgaagc ccgacacggt ccaaaacatc
     4081 cttgtggtca ctgacgggac cttcctcagg ggaggggacg gggccctggt cgggccgacc
     4141 ctcgcaaacg acttcagcct gaaggtgggg agcaggatca agctcggttc ctcttcggac
     4201 aactcgacga ggaatgtccg ggtcgtcggc atcctccagt ccacgggatt cggggccggc
     4261 ctttccaccg acagggccat cgtcgtcacc gacaagtggt acacggccgc ctacggggga
     4321 tccggagaat attcccaggt caatgtcatc gtcgggaaca tcaaccagat cggcaccatc
     4381 gagaacaaca cggataaggc gctgaacagg aaccagaagc tccccgccgt gaggataacc
     4441 gattcgggtc agttcctcac gaccatcacc caggccctcg ggagcatcac ctccttcgtc
     4501 accatgctcg gggccatctc cctcgtcgtt gcggcggtga gcatcttcaa cgtgatgatc
     4561 atgtccgtct ccgagcgggt ccgggagatc gggatcctcc ggtccatcgg gacgaggaag
     4621 agcgagatcc ggaagatgtt cctctacgag gccgtcatca tcgggctcat cggggcggtc
     4681 atcggggccc tcatgtcggt ggtttccagt tacctggtgg tcacggcgat agtcgggaac
     4741 acgacatatt tctttacgcc ggcgagcctc gcctacatcc cgcaggggat gctcgtcggt
     4801 gttgccacct gcgtcctctc cggcgtttac ccggcgtgga gggccgcgaa cctcgacccc
     4861 atcgaggccc tgcgggcgga gtgacatcgg ggtgcgagcc accggcggag ccggttttgc
     4921 gagcagcccg gtgtggagtc accgggtgca tccacacccc ggtgaggagc acgtcgcagc
     4981 cgatgtgcga ccaaatggcg gagccggttt gcgactaggg atatttcaga ggtccctcat
     5041 catttgtatc gcgcagaatt cgccgcacat ggtgcagggc ccgtcagcgg gcgcgagcgc
     5101 ccttgccgtt tccccgtcga gcgcacgaga gaactgcccc tcccagtcca gatctgccct
     5161 cctccttgag agctccctgt cgcgggcatc cggtccgtat ttgatggaat ccccgatgtg
     5221 ggcggcgatc cggaaggcca tcagcccctc cttcacctgc ccgggatccg ggagcccgag
     5281 gtgttctgcc ggcgtgatgt agcagaggta atcggcaccc gcccctgcag cgatgcttgc
     5341 gcccacgcac cccgccatgt ggtcacggcc gacggcgatg tccgtgggga gggggcctgc
     5401 gacaaagagg ggggcctctg acagcctccg gtaccgcctg atatacgccg cgatccggtc
     5461 ggcccgcaca tggcctccga tgccctcgat gatggtctgc acaccggcgt cgtgggctcg
     5521 tctcgcgagc gacgcgttca gcatcatctc ccccttctgc gcccgatcca tcgggtcatg
     5581 gatacacccg ctccgcatgg tatttccgag ggagaggacg atgtcacgcc ttttgaggat
     5641 cgagaggacc tcgtcgaagt gctcgaggaa ggggttctcg gacccggtcc gcatcatcca
     5701 ggcgcaggtg atggacccgc ccttcgagac cactcccatg atccgtttcc ctcccctcag
     5761 ggtgtcaagg aggtcccggc tcgtgcagtg gagcacgacg gaggagaccc cctcatctgc
     5821 ctgctccctg aggatccgga ggatctcctt tgtggtcatg gtgccgatgc cgatccgggc
     5881 cgccgcctcg tagatcggaa ccgtcgtaag ggggagcttc gtggcccgga atatcttcct
     5941 ccggatgtcc cggatgtccc ctcccatgga gagatccgtc agggtgtccg cgccatattt
     6001 ttcggcgatg accgccttct ccacctccgc ctcgggatcg attcggagag gcgaagtacc
     6061 gatgttcacg ttcacctttg tccggagacc gtggccgatc ccgaggcacc ttttccccct
     6121 ccgcatgatg gtgacagagc ccccggcgat accgtcgagg atgagggagg gatcccgcat
     6181 ctcggacccg gccacggttc tcaccggccc ggtcacccgg ccttcccgcg catgatcaat
     6241 gagggtcttc atcaccgggc ctcttccggg cccttccggc cgaacaggtg ttctgcgggg
     6301 cagcccacct gcctctgccg gcagtaccgg caggcccacc tgctccggat gaatccggtg
     6361 ccgggaaatg ccaggagagc gagggccacc acaaccgcga ggaggagaac atcgaacctg
     6421 aggacgagga ggatgatccc caggaggagg ggaatggtgg agatgaggaa gtcggggagg
     6481 atgtccaccc atccgatctt cctctcccgg aatcttttcg ggtcaccctt ccggaagata
     6541 agggcacaga gccttcccct cccgaaacag caggtcatcc cgtagtagga gcagttcacg
     6601 caggagtgcc ggaggacgcg gtattccagg ccaaggacgc aggcgatata gatcgcgagg
     6661 aagaccagtc cgaactgcag gacgatgtac gccccgagga tatagatcgc agcctgaacg
     6721 aggagggtgg atgcgacaat ccggaggggg aagttctcga agctgccgtc cttcagtgcg
     6781 gtatccacga tcatctgaag ggaatccggt ccaataaggg tgtcgggtcc gtcgaggtga
     6841 cccgacctac cctgcccgtt cgaaacaaac cttcttataa tgcggagaaa gagaggcctt
     6901 taggtgaacc catggggagc ctggaagaga acatcggtta tcacttcagg gatccggccc
     6961 tcctcaccag ggcgctgacg agaaaggcat ttgcgaacga ggagatgcat cagggaaggc
     7021 agcacgaaga ccaggagatc ctctccaccc tcggggatgc ggccctcaag gcggccttcg
     7081 tggatctcct cgtccggtcc cattacccca cgccggatgc catcacccgg gagaaggaga
     7141 gccttgaacg gcatgacgca ctcgccggta tcgccctgca gatcggaata ggcccctcca
     7201 tccggctcgg gagcggggag aagaaacggg aggcagagca ggaaccttac gttctcgcgg
     7261 agaccctgga ggccattatc ggggccatct acctcgatgg aggatgcgag gccgcacgga
     7321 acgcggtcgt gaggtggtac ggtcacagga tcaggccaca tccaggatga caccttcttt
     7381 cctgaaccgc aaggattctg cctcctccat ggggggatga ctacttcccc ttggaacgcc
     7441 aagtactctc aggtgattcg ttcacagggg gatgaaggga tgatgttcag ggcggctccc
     7501 gttcacgagg acgggagaca gagggaaagg ggggaatatc ccgaacggcc tgaacgcatc
     7561 catctcttcc gatagtctgt gttctctatt gccgggacga aacgtgacag gaatgctacc
     7621 ccagaccaag gcctttgtgc ctgattccgc ccagatcatg atcctgcggg aagtttcaga
     7681 ttgcccgagc tgctccataa cccacgtcgt cgagaagctg ctgcccagcc acagcgagag
     7741 cattgtccgc tccagggtcc gcgagctgct cgcccggagg taccttgagt gggggaagtc
     7801 acccagtgca attcagctga ggctcacctc gcagggccgc gtggccctcc agcgggaacc
     7861 ggtctgaccg gcctcccggg atttcatcag agtctttttt cgggcaagag cggggcagag
     7921 atccgaatag tcccggactc ccctctggcg aatcctccgg tgaaaaggtg tttctattca
     7981 atcactcttc agaaatggat gcgaggggtg agatttgaac tcacgaaccc ctacgagaat
     8041 ggaccctgaa tccatcgcct ttgacctggc ttggcaaccc tcgcgcagct acagaatagc
     8101 gtaccagacg tcataaagat agtgataccg gcccctcgac gacggatcgg tttccgttgt
     8161 gactcggaaa tccggaagaa tcgcataatt caccttcatg gcgtgctctc gggggaggga
     8221 gggttcatca gaacccgacc tggagcgacc cgccatcagg cgggtgcgac cgggaggttt
     8281 cgtaagaggg aggtttcgtt agaaaccgac cttgagcgac gcagtcgtga gaaaccgacc
     8341 tcgagcgacg aagtcgcgag ttggcaacgc caccccgccg cgcgggcacc ccccgcttcc
     8401 ggcatctcct gatctggcag tttctcagtg aaacggtcta ccccaccata gggcgcccag
     8461 cagaggggaa gcggagcaac cccccggagc atcgacgaag taattgtccg gaaatttacc
     8521 gttcgtgccc gtggtgaatg tcaggtgcag ggggattctc atgatctacc ggttcgtgaa
     8581 catgctcatg cacgtgttcg ccggtatctt ccccggggtg aggatggtcg tggagttcat
     8641 catgcctgtg ccggtgtgcg tgcacgatgc gttcatggaa atgtccgggg atatggcgct
     8701 cgctcacgag aagtgccacc cccaggagga agaggggaag ggcgataagg aattgcgtgc
     8761 ccgggacctc ccggaggaga agcagggagg cgatgctgcc gatgaaaggc gccgaggcaa
     8821 aatacgcgct cgtccgggtg gcgcccagat cccggagcgc cctcacgaaa aagacgatgc
     8881 tcaatccgta ggtgacaaac cccacgatca gcgctgccgc cacgacctgg aatccaggca
     8941 cggcggcacg ggtgagcatg gcgatgagga acgagacccc tcccgcgccc atccccttca
     9001 ccacggcgat ggagagggga tcctttcccg agattttcct ggtcaggttg ttgtcgatcc
     9061 cccagagggc acaggccgct acgataccca gagaggcggg cgagaatccg ggcagaccgg
     9121 tcatgttcca ggtaagaatg atgcccgcca cggtgatcac cgcgatggcg gcgagaactc
     9181 tcctcccgat gaattccgcg aagatgaaaa aggcgatgag ggtcgtggcc acgagctcga
     9241 agttgaggag aagcgacgcg gtggcggcgg aggttgcacg gaggccgtag aggaggctga
     9301 tgggtgcaag gaccccaccg gtgaggatcg caccggtaag ccagggataa tcgcgggcat
     9361 cgagcctgag ctccctggga gctttcccgt ctggacgccg gaacctcccc aggagaagta
     9421 cccccgtgcc gctcccgagg tacaggagcg cggcgaagag gatgggatcg atctccccga
     9481 gcaggatctt cgcgagcggg gtgctcgcgc cgaagagaac ggcggccgcg acggcataga
     9541 ccggcccctt cgaaaacgag ctccccgtca tatctcccgc agaggggatt cgtgccaagc
     9601 cgtataaagg tgcggcagcc ggcgagcatg cgaggtccgg ttctgacgaa cccgggtcgc
     9661 accccgcatg atggcgggtt gctccaggtc ggtttcttcg cgacttcgtc gctcgaggtc
     9721 ggtttcttac gaaacctccc tcttacgaaa cctccctctt acgaaacctc cgggtcgcac
     9781 cccgcctgat ggcgggtcgc tccagctcgg tttcttcgcg acttcgtcgc tcgaggtcgg
     9841 tttcttaccg aaacctccct cttacgaaac ctcgcttacc aaaacctatg cgtccggatg
     9901 aagttccgct cggccttcag gatctcccgg tagaaggcgt ccccctcggt catggtagcg
     9961 aggata
//
LOCUS       DATGDI010000160        20496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_365919, whole genome shotgun sequence.
ACCESSION   DATGDI010000160 DATGDI010000000
VERSION     DATGDI010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 20496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20496
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365919"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..577)
                     /locus_tag="VKO45_06070"
     CDS             complement(5..577)
                     /locus_tag="VKO45_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HVO_0476 family zinc finger protein"
                     /protein_id="HMA05477.1"
                     /translation="MDITLTCPSCGEECGHVLVDESRNPVVRCTLCGHTHRGVPPKEK
                     EIPVRVIESEEGESRVCSVSLEPGEIVAVGDFLAAECGEEASGVEVTSIESKGKRVRK
                     ARARDVSTLWTRAIDQVVVRASVHQGWVTRAVSVVCDGGEEFSVGESWTFGPLPFRIT
                     HMKLRNGKVLRKEGQRAPALAVKRIYGNRL"
     gene            718..1158
                     /locus_tag="VKO45_06075"
     CDS             718..1158
                     /locus_tag="VKO45_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF371 domain-containing protein"
                     /protein_id="HMA05478.1"
                     /translation="MKAVERIFCRGHPLVLATHPTTFEVTTEDHLTRQGDCIIGIRAD
                     RGAADLAPGFRRVLADDRAVLLTRLTAGGYTVEVHGKGSARMTIAHPTDLVWRRSGFV
                     CPRTVAIHCDRVALTLPRDLIRALREGAELEVELVAAMEEVTPR"
     gene            complement(1145..1327)
                     /gene="xseB"
                     /locus_tag="VKO45_06080"
     CDS             complement(1145..1327)
                     /gene="xseB"
                     /locus_tag="VKO45_06080"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HMA05479.1"
                     /translation="MTRKFEEMLGELKDVVKKMEDPDTGLDESIALYERGAALIRECE
                     KKLAEAELKVTEVTEG"
     gene            complement(1311..2558)
                     /gene="xseA"
                     /locus_tag="VKO45_06085"
     CDS             complement(1311..2558)
                     /gene="xseA"
                     /locus_tag="VKO45_06085"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HMA05480.1"
                     /translation="MEGVPGGGQAVPAGPAVLGVAEISGIISEALDDERLRGIWIRGE
                     ITSYKHHHSGHRYFSLSEQVWGRNFSIRCIMWESRARELDFEPKDGIAVIALGSVQVY
                     EARGDLGFYIEEMLPAGAGEKHLLVERWKKELAAEGLFSEERKRPLPPFPSRVGVVTA
                     RTGAVIHDILHVIGRRYPVEIVLSATAVQGDLAHLEIARAIQVLDGRVDVIILARGGG
                     SFDDLFPFNHPDVVRAVGCSQTPVVSAVGHEVDHVLADLAADRRAPTPSAAAELVVPD
                     RADLGRELAGNLDRLRSSLGKKVQDAGLLIEDLRLRLQPRRLVRRINENRELVAGFEE
                     DLDRVIAAKIGRLRAGLEREKALLEGRSPLSILSRGYCIAEKEGRPVRTARDLRKDDR
                     IRLRMAGGGAKARVEEVFHDKEV"
     gene            2609..3439
                     /locus_tag="VKO45_06090"
     CDS             2609..3439
                     /locus_tag="VKO45_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HMA05481.1"
                     /translation="MSGMVNIAVSTMFFHEYPLGEGFDAVERAGLDGIEFWVETPSFW
                     LYGHPVGELLARLGDHDGFGPVNLHAPILDLNPCSVNPRVARASLAYTGEAVELAGRI
                     GAGVLTVHPGRRTAKRPPSAPEYERFERLIDLLREGAGRNRVRIAIENMERAVNSLLS
                     TPDEVRELLDREPWLFFTLDVSHAMARSVEEVTDFIDLCGDRMVNIHLARSEGGRTHL
                     PLDGSTRGRVVLHALAEAGYTGPLTLEIEDRNFDHDLSLEEKVALLSREAVFIRSEFG
                     "
     gene            3537..4799
                     /locus_tag="VKO45_06095"
     CDS             3537..4799
                     /locus_tag="VKO45_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HMA05482.1"
                     /translation="MASEIELLAFFLCIILSAFFSGSEVALLSITHAKVRTLLARGGG
                     SAQALADLKRMPDHIIITVLIGNTLVNVGAASIATSLAIERFGDTGIGIATGIVVLVL
                     LIFGEIGPKMLAVRFTEPVALGVSRPIRFLATVFSPFIWVFDRLARSMELTKAFAKPA
                     ITEEELRGWIELGQEEGTIEKGEQQMLFNVLEFGDTTAREVMTPRVSVTMIDEHDSLT
                     QAVSIFKDTGFSRLPVFSGEKGNVVGLLNVKEVFPVVLEEKQGVGIGDLMHEPFFAPE
                     TAKIDDVLKELQKRKVHMGFVLDEYGEFTGVITVEDILEELVGEILDEFDQDEPEVQV
                     VEPGVYLVDGQVRVKDANDRLGISLPLETSYETLGGLVIDRLGHIPRRGETVSLDGTA
                     LTLTIIQMLGRRVGTVKVTVSPPIHGEG"
     gene            4803..5426
                     /gene="purN"
                     /locus_tag="VKO45_06100"
     CDS             4803..5426
                     /gene="purN"
                     /locus_tag="VKO45_06100"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HMA05483.1"
                     /translation="MGRASAAPKRIAVLASGRGSNFQALIDAVRAGSIPATIVRLITD
                     NPEAHAIHRAGEAGIPVSVLAFGRFPGKEAYEAALKREMEDCGADLYVLAGYMRIVPG
                     AIVKAFPGRIMNIHPALLPSFPGLHAQRQALVYGVKVSGCTVHFVDEGTDTGPIILQR
                     CVKVAAGDDEESLSARILVEEHRILAEAVRLFCEGKIRLAGRKVRIG"
     gene            5497..5919
                     /locus_tag="VKO45_06105"
     CDS             5497..5919
                     /locus_tag="VKO45_06105"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF015965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component 4"
                     /protein_id="HMA05484.1"
                     /translation="MGRRSQTPESRRLARERIEILFDRAREFFPENPALSDRCVDLAR
                     RIAMRQRVRIPRELRRRFCRKCSAYLVPGSTARVRIRRGKMIMTCLRCSGHRRFRLGG
                     REGIRARAAVERGSGTERSSGDPGPAGVTKGSGTGGST"
     gene            5916..6146
                     /locus_tag="VKO45_06110"
     CDS             5916..6146
                     /locus_tag="VKO45_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HMA05485.1"
                     /translation="MTDTSPMQTLKPTVWIGKKGGTPEAMAEIRRQLEAKKPVKVRFL
                     RGAEMDPEALAAETGGEILGVRGRTVVLRKER"
     gene            6286..6735
                     /locus_tag="VKO45_06115"
     CDS             6286..6735
                     /locus_tag="VKO45_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
                     /protein_id="HMA05486.1"
                     /translation="MTTLYDIPAGLLIPRLAEELKKRKEIQPPAWAAFAKAGVHQQMP
                     PEDPDWWFIRAASVLRRLYIDGPVGVQRMRTAYGGKRDRGSSPFQFRKGGGSSLRKVL
                     QQLEAAGLVAKDKTGRSLSVEGRKFLDGIAHGLRPVAQEKAEGLSKY"
     gene            6748..7086
                     /locus_tag="VKO45_06120"
     CDS             6748..7086
                     /locus_tag="VKO45_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HMA05487.1"
                     /translation="MGDDELAELRRRRLEQLQRQSADQQVLEEQARREQQVEAQIHHI
                     LLQILEPEARERLANIKLTKPDFARAIEQQLVALAQSGRLKQKITDAQLKELLRQLVP
                     QKKDWNIRRA"
     gene            7133..7285
                     /locus_tag="VKO45_06125"
     CDS             7133..7285
                     /locus_tag="VKO45_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HMA05488.1"
                     /translation="MSRVTKGLKIRLAKACEQNRRVPAWVMMKTKRAVSTHPKRRTWR
                     RSTLKV"
     gene            7289..7552
                     /locus_tag="VKO45_06130"
     CDS             7289..7552
                     /locus_tag="VKO45_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HMA05489.1"
                     /translation="MVEKLKEQIYIIPLRDVQHTPRWERGKRAMKAVHDYLARHMKSE
                     DIKIDQSINEAVWERGSHKPPSRIRVRAMKFEDGQVQAELAKE"
     gene            7554..8216
                     /locus_tag="VKO45_06135"
     CDS             7554..8216
                     /locus_tag="VKO45_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HMA05490.1"
                     /translation="MSSTIAFAGDPHIGVFVRVLEDLVLLAPLAPEGFRAAIEEELEA
                     EVVETTIQDSAIVGALAAGNRRGVVVSGLASRDEVARIEEHREVMVLGDGMNAAGNVI
                     LANDVFAAVNPEMPLDIAEEIGSFLRVPVLRLSFAGYRAVGKVAVATNRGILVHPRAN
                     PAEIAALEQAVDLPIGVGTVNLGSGLVGSGLIANSRGYLAGVGTSGFELGRIEEVLGF
                     LE"
     gene            8223..8414
                     /gene="rpl18a"
                     /locus_tag="VKO45_06140"
     CDS             8223..8414
                     /gene="rpl18a"
                     /locus_tag="VKO45_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18Ae"
                     /protein_id="HMA05491.1"
                     /translation="MSSQSYRVEGTARIDGEWRPYRKVISAPNEKQARERVYTLLGSK
                     HRLPRREIRIGKIEPAEGE"
     gene            8416..8844
                     /gene="pfdA"
                     /locus_tag="VKO45_06145"
     CDS             8416..8844
                     /gene="pfdA"
                     /locus_tag="VKO45_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HMA05492.1"
                     /translation="MAEPEADLQTLQYYLNEYSQQAEIFSRQLEMIEQKRIESLAAIE
                     TMKALVADRDAPTLLPLGGGTMVRVKVGDPEKVLVNVGSDVIVQRTNGEAITFLQEKI
                     TEMEAMEKKVAQTIDQLRAQMSGVARQIESAYAAQERPQG"
     gene            8848..9846
                     /gene="ftsY"
                     /locus_tag="VKO45_06150"
     CDS             8848..9846
                     /gene="ftsY"
                     /locus_tag="VKO45_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HMA05493.1"
                     /translation="MFDGLREKLRSVQEVFGTSIRAASPSPAGETPEAKAGPGLAERV
                     KILVTDGELVLSEKQIEGPLQELEMVLLENDVALPVTDEILRRVKEDLVGKHRRIGES
                     PEEVILSALRDAIVRVLGEGFDLAAYIRGRERPVKILFTGVNGTGKTTTVAKVGDYLK
                     RLGFSVVIASGDTFRAGALEQIATHAERLGIKVIQHKAGGDPSAVLFDAVEYAKAHRM
                     DVVLADSAGRFHNRVNLMNQLEKMKRVMSPDLVVYVDEAVAGNDAVVRAEEFERLVGT
                     DAVILTKADMDMRGGAAISIAHTVGKPIMFLGTGQGYDDLMPFTPGGLVDELLGGA"
     gene            9846..11159
                     /locus_tag="VKO45_06155"
     CDS             9846..11159
                     /locus_tag="VKO45_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein Srp54"
                     /protein_id="HMA05494.1"
                     /translation="MLDKLSSSLKDALKKLAGKAVVDRAAVEELIRDLQRALIQADVN
                     VRLVQGLSQAVKTRSLDEQPPKGMSVREHVLRIVYQELLRLMGARAEVALGPQRILLA
                     GLQGSGKTTTTAKLARYFQRKGMKAGVICADTFRPGAYDQLATLCGQSNIPCYGEPAE
                     KVAEGIVERGLGSLAASEVILIDTQGRHALEKDLIEEIIRLNAISRADHRWLIIDAAL
                     GQQARDQARRFHEAIGIDGVIVTKMDGTAKGGGALSAVSETQSGIVFIGSGETIDDLE
                     RFDPDSFISRLLGMGDLAALAERAEEAISTEEMDVQGMLRGKFTLRDMYKQLDAVSRM
                     GPLKQIMSMLPLGGTDVPSEAIDVTSSKMARYRVIMDSMTEQELDDPNVISGSRIHRI
                     AAGAGSSPDEVRELLKYYRLMQRALKGMRGGKFNLQRLMRRFSGM"
     gene            11161..11739
                     /gene="trmY"
                     /locus_tag="VKO45_06160"
     CDS             11161..11739
                     /gene="trmY"
                     /locus_tag="VKO45_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (pseudouridine(54)-N(1))-methyltransferase
                     TrmY"
                     /protein_id="HMA05495.1"
                     /translation="MRSFAVVGHLAPTGDRFTLDDLPGSAGRLDILCRCVNAALSLSH
                     GIRRDAGIHLILLGPPDPPRDVLISGASVRSLSPDERSTAALIRKALALPVGEAFRES
                     GPGISVRRGNLAGLVPGGGYTVLSETGEDVRGVKNLPAGFILSDHLDFTPEEEAILAG
                     LPRISVGPRVLHADHAITVLQNELDRREAGWR"
     gene            11730..12962
                     /locus_tag="VKO45_06165"
     CDS             11730..12962
                     /locus_tag="VKO45_06165"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(54/55) synthase Pus10"
                     /protein_id="HMA05496.1"
                     /translation="MEIEEQVDRILAYGEICDHCLGRFFGKKSHGLANEERGHGLRVA
                     RALRVNTPFVPPSGPCWICHDLFAGIGTWAERVVQALSGIEYSTFLIGTRVPPLMAES
                     EELVWSDLGLDGPEPLKSEMNREVGKVVSAITGRAADLARPDVVAILDLARETVELEI
                     SPLFFRGRYRKLERGIPQTRWPCRECHGAGCERCGFTGKMYPDSVEELVGRTAAGVFR
                     ADDAVLHGAGREDIDARMLGTGRPFVMEIVAPRRRVVDLAALEERVNTAAAGRVGITI
                     EGWSTRGEVETLKSEKAHKKYRISVEIEGGIARKELEEALDRLKGAVVSQQTPARVVH
                     RRADRIRERRVVDARVAGQEGGTFFLDVVGEAGLYVKELVSGDGGRTRPSLAGLVGRP
                     ARVVQLDVLEVEGSEERE"
     gene            12966..13256
                     /locus_tag="VKO45_06170"
     CDS             12966..13256
                     /locus_tag="VKO45_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21e"
                     /protein_id="HMA05497.1"
                     /translation="MAHHHGPRKKTRYKFKKEIRRRGIPPVMTAIQKFEVGQKVHIVC
                     EPSVHEGMPNRRFHGMTGTIVGQRGRAWLLQIRDGESTKTVIARPQHLKAQR"
     gene            13280..13630
                     /locus_tag="VKO45_06175"
     CDS             13280..13630
                     /locus_tag="VKO45_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb4 family protein"
                     /protein_id="HMA05498.1"
                     /translation="MRVKAVLGEERVTLAELKETLTTVEESRREDATEMAYELRRSIE
                     HATHLTKTTPKQAKALLSALLALEKMKPDIAYRITNIMPRTRDELRAIYAKERFTLTG
                     EELDQILSLVSEHL"
     gene            13641..14204
                     /locus_tag="VKO45_06180"
     CDS             13641..14204
                     /locus_tag="VKO45_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HMA05499.1"
                     /translation="MRTEKKENYAIVLDILQKGHADDPRPVYKREPLVQALGIEQFKI
                     LELVPKPNAVIQIHEKLYIGDEEREKIERVKRRIGFEELTPTARVEVPYVVEQIVKEH
                     EDRFVQFFNTSVPVTPKLHMLHLLPGIGKKLLWEILDERQRQPFMSFADIAQRIKAMP
                     HPDRMVINRVLEEIEDPETKYRVFTAR"
     gene            14201..14965
                     /gene="rsmA"
                     /locus_tag="VKO45_06185"
     CDS             14201..14965
                     /gene="rsmA"
                     /locus_tag="VKO45_06185"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HMA05500.1"
                     /translation="MTARRDQHFLVDERAVERILSLVNVAGHTVLEVGPGKGILTRGL
                     LDRGAEVVAVEVDPFLVEELRGTFAPEIADGSLHLIEGDAVKVELPPFELVVSNLPYS
                     ISSPITFRLLERGFSEAILMYQSEFADRMVAKIGTPACGRLTVMVQTYAEVRKCIHIP
                     PNAFSPRPQVHSTVIRMVPREPPYPTGDRRHYADVVRVLFTQRRKTVRNGLRSGKGIF
                     GADRVERMIAATPPEVLGLRPEALSLRDFAAIARVE"
     gene            14966..15526
                     /locus_tag="VKO45_06190"
     CDS             14966..15526
                     /locus_tag="VKO45_06190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK2/MTQ2 family protein methyltransferase"
                     /protein_id="HMA05501.1"
                     /translation="MEADPDQVYPVREDTLLLLEAALAEAGPADRVLELGTGSGYIAM
                     RLAGRVSFIVATDLNPHACRLASAQGLGTVRADLTAGIRGSFDLILFNPPYLPTKPGE
                     RLDDWLEKALDGGESGRDVIANLIPDLPRILAPGGRLLLVISEVTGVPEVLDLVTRAG
                     FSLEIARRVPIEGEDLMVLKAIRRRD"
     gene            15594..15995
                     /locus_tag="VKO45_06195"
     CDS             15594..15995
                     /locus_tag="VKO45_06195"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMA05502.1"
                     /translation="MSPRERRIPEDAQAVIDHAVSGIPKGKVIAIFLYGSVARGEEKP
                     LSDIDLCVITGRGISRAERERILVHASPTLDITLFWDLPLPIRFRVLREGRLLWGEED
                     LELHRIRRGTLRCYREIGRLIRRHERMMLDA"
     gene            15988..16437
                     /locus_tag="VKO45_06200"
     CDS             15988..16437
                     /locus_tag="VKO45_06200"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HMA05503.1"
                     /translation="MHKPYDREQVTKIISDIERYTKDLQELGISGREELEDRRTLYAL
                     SMLLFTIINRALDLADEVIITNRLGMPQSYREIFDILRRRRFIDRELAEEMAALVFYR
                     NMIAHQYDDLTDDDLYSLFQRLGTITKFVDRMKEIIREGPQARGKKR"
     gene            16511..17761
                     /locus_tag="VKO45_06205"
     CDS             16511..17761
                     /locus_tag="VKO45_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HMA05504.1"
                     /translation="MGATYVEKIFSRGCRREARAGEVVMAPVDAAMIHDITGPLAIQK
                     FREMGGKRVFDPAKVIMLFDHQVPADSIPAAQNHIFMRMFAEEQGIGNLDLNEGVCHQ
                     VVEERGLAAPGEIVVGADSHTCMYGATGAFATGIGSTDMGFVLKFGALYFRVPETIRV
                     EVSGVFPNRVGPKDLILAITGDIRADGATYRALEFTGETMGGMGMAGRMTCCNMAVEM
                     GAKAGIVPPDKVTWEYMKGRRRIKPFDLAADPDASYLEERSWDVSDLAPKVAVPHNVD
                     HVVDVGEVAGTHVDQVFIGSCTNGRFEDLKEVADVMGDRKFSKGLRVIVIPASRTEYL
                     RALRAGFIERFVEAGALVEAPCCGPCMGGAFGLLGPGEVSLSTSNRNFKGRQGSAEAK
                     VYLASPSTAAATAIMGEITDPREV"
     gene            17818..18324
                     /locus_tag="VKO45_06210"
     CDS             17818..18324
                     /locus_tag="VKO45_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HMA05505.1"
                     /translation="MSAGRVWRFGDDVDTDAIIPGRFLTLYDPRELAKHAFEGTRDEF
                     AGGAREGDLIVAGRNFGCGSSREHAPLALKGAGIRVIVAESFARIFYRNAVNSGLLPL
                     VCTRARDIRDGEEATLHLEAGTLTVGGRRFTVEPVPDFLREIVDAGGLVEFARGLSEV
                     VTCRDITT"
     gene            18303..19436
                     /locus_tag="VKO45_06215"
     CDS             18303..19436
                     /locus_tag="VKO45_06215"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HMA05506.1"
                     /translation="MPRHHDVAVVPGDGIGPEIIAEGRKVLDAASERFGFDIRWRDLD
                     IGAERYLRTGELLTEKDLATLGTCRAIYFGAIGDERVKPGVLEKGILLALRFHFDLYV
                     NLRPVKLLPGVWTPLADKGPADVDFVVVRENTEDFYVGIGSRFRGEERKALEVIRSLY
                     RVKFGIDAESDAEEVAYQIGVVTREGTRRVIRFAFDEALRRGRAAPKVTSVDKANVLT
                     EVYGLWREVFSEVAKGYPGVSSEFSFVDAITMWFVKNPESFDVVVSPNMFGDIITDLG
                     AMIQGGLGLAPGGNINPDGTSMFEPIHGSAPKYRGLGVANPVATIWAGSMLLDHLGEH
                     EAAAAVLDGIGKAIGAGVKTKDMGGRSRTAEVGDSIAAYLGNL"
     gene            19571..19999
                     /locus_tag="VKO45_06220"
     CDS             19571..19999
                     /locus_tag="VKO45_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05507.1"
                     /translation="MTEALVKDVSGLIGIWQPRKAYHIYPTYLEDLKKFLQERMKAPG
                     SFFSGKRANIEFVDGKGMNIGVKRKAGSAVEAVGIVMKRDLITLDDLKLLMDQINQAE
                     QMYPELFVILMGTQTADMESKLGTFLANKDGKAKITVIKK"
     gene            complement(20011..>20496)
                     /locus_tag="VKO45_06225"
     CDS             complement(20011..>20496)
                     /locus_tag="VKO45_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HMA05508.1"
                     /translation="GIDIGPVLAEAARAEERIGRAADKYLRRFDPPRAAVFGGWAYAG
                     LASGILKKYLDAEIVVTGSRNGIRPSPQPVREMADLGGIHEFLSAEKPDLIVGSSFER
                     SACPGAAFVGITPPLRGRVLLRSRALAGIEGSLSFMEEVLNACMDRNRRDSGSSGTRM
                     A"
BASE COUNT         3682 a         6574 c         6963 g         3277 t
ORIGIN      
        1 gtacttataa ccggttcccg tagatccgct tcacggcaag ggcgggagct cgctgccctt
       61 ccttgcggag caccttcccg ttccggagct tcatgtgggt gatccggaaa gggaggggac
      121 cgaaggtcca ggattccccg acggaaaact cctcgccccc gtcgcagacc accgaaacgg
      181 cgcgggtgac ccatccctgg tggaccgagg cccggacgac cacctggtca atggccctcg
      241 tccagagggt cgagacatcc cgtgcccttg ccttcctgac ccgctttccc ttcgactcga
      301 tagaggtcac ctcgaccccg gaggcctcct ccccgcattc ggccgcgagg aaatccccga
      361 ccgcgactat ctcccccggc tccagggata ccgagcagac ccgcgactcc ccctcctcgc
      421 tctcgatgac cctgaccggg atctccttct ccttcggggg caccccacgg tgggtgtggc
      481 cgcagagggt gcagcggacg acggggttgc gggattcgtc cacgaggacg tgtccgcatt
      541 cctcgccgca ggaggggcag gtgagggtga tatccatttt atatgaatga gtgcctcttg
      601 ctcaaaaatg ttcattttct cgaagggttc cccttgctca aaaatgttca ttttttcaaa
      661 ggggtcccct tccggaaaat gatttttaag ggaaggggcc ccttccctac ataatacatg
      721 aaggcagtgg agaggatctt ctgccggggc catcccctcg tcctggccac gcacccgacg
      781 accttcgagg tgaccaccga ggaccatctc acccggcagg gggactgcat catcggcatc
      841 cgggcggaca ggggggcggc ggacctcgcg ccggggttca ggagggtgct cgccgatgac
      901 cgtgcggtcc tcctcacgag gcttacggcg gggggataca cggtggaggt gcacgggaag
      961 ggctcggcac gaatgaccat tgcccacccg accgatctcg tctggcggag gagcggcttc
     1021 gtatgcccgc ggaccgttgc catccactgt gaccgggtgg ccctgaccct tccccgggac
     1081 ctgatccggg cactcaggga aggggcggag ctggaggttg agctggttgc cgcgatggag
     1141 gaggttaccc ctcggtgacc tcggtcacct tcagctccgc ctcggcgagt tttttctcgc
     1201 actcccggat gagggccgcc cccctctcgt aaagggcgat ggactcgtcg agccccgtgt
     1261 ccgggtcctc catcttcttc accacgtcct tcagttcccc gagcatctcc tcaaacttcc
     1321 ttgtcatgga acacctcctc gacccgcgcc ttcgccccgc ccccggccat ccggagccgg
     1381 atccggtcgt ccttcctcaa atcccgggcc gttctcaccg gcctcccctc cttctcggcg
     1441 atgcagtagc cccgggagag gatggagagc gggctgcgac cctcgaggag cgccttctcc
     1501 ctctcgagcc ccgcccgcag ccgcccgatc ttcgccgcga tcacccggtc caggtcctcc
     1561 tcgaacccgg ccacgagctc ccggttctcg ttgatcctcc tcacgagcct cctcggctgg
     1621 agccggagcc ggaggtcctc gatgagaagg ccggcgtcct ggaccttttt cccgagggag
     1681 gacctcaacc ggtccaggtt gcccgcgagc tcccggccca gatcggcccg gtcgggcacc
     1741 acgagctcgg cggccgccga gggggtgggg gccctccggt ccgcggcgag gtcggcgagg
     1801 acatggtcca cctcgtgtcc caccgccgag accaccgggg tctgcgagca cccgacggcc
     1861 ctcaccacgt cggggtggtt gaaggggaag aggtcgtcga aggacccccc gccccgggcg
     1921 aggatgatga cgtccaccct cccgtcgagt acctggatcg cccgggcgat ctccaggtgg
     1981 gcgagatccc cctgcacggc ggtggccgag aggacgatct cgacggggta gcgccggccg
     2041 atgacatgga ggatgtcgtg gatcacggcg ccggtccggg cggtcaccac ccccacccgg
     2101 gagggaaagg gtgggagggg cctcttcctc tcctcggaga agaggccctc ggcggcgagc
     2161 tccttcttcc atctctcgac gaggaggtgt ttctccccgg cgccggccgg gagcatctcc
     2221 tcgatgtaga accccaggtc gccccgtgcc tcgtagacct ggacggagcc gagggcgatg
     2281 acggcgatgc cgtccttcgg ctcgaagtcc agttcccggg cacggctctc ccacatgatg
     2341 caacggatgg aaaagttcct cccccacacc tgctcggaga gggaaaagta gcggtggccc
     2401 gagtggtggt gcttgtagct cgtgatctcc ccgcggatcc agatcccccg gagccgttcg
     2461 tcgtccagtg cctcggagat gatccccgag atctcggcaa cccccagcac cgcgggcccg
     2521 gccggcaccg cctggccgcc cccggggact ccctccatca atgcctatta tgatggtagc
     2581 cgttatgaac atgtgggatg aggggtgaat gtccggtatg gtgaacatcg cggtctccac
     2641 catgttcttc cacgagtatc ccctgggcga gggcttcgac gccgttgaga gggcaggcct
     2701 cgacggcatc gagttctggg tcgagacccc ctccttctgg ctgtacggcc acccggtcgg
     2761 cgaactcctg gcccgcctgg gggaccacga cgggttcggg ccggtgaacc tccacgcacc
     2821 catcctcgac ctcaacccct gctccgtcaa cccgcgggtg gccagggcga gccttgccta
     2881 taccggggag gccgtcgagc tcgcaggcag gatcggggcc ggggtcctga cggtgcaccc
     2941 cggccggagg accgcaaaga ggcccccgag cgcccctgag tacgagcggt tcgagcgcct
     3001 catcgacctc ctcagggagg gtgccgggag gaaccgggtg aggatcgcca tcgagaacat
     3061 ggagcgggcg gtgaactccc tcctctccac accggacgag gtgagggagc tcctggaccg
     3121 ggaaccctgg ctctttttca ccctcgacgt gtcccatgcc atggcccgat cggtggagga
     3181 ggtgacggac ttcatcgacc tctgcggcga caggatggtg aacatccacc tcgcgaggag
     3241 cgagggcggg aggacccatc tccccctcga cgggagcacc cggggcaggg tggtgctcca
     3301 cgccctcgca gaggcgggct acacggggcc cctcaccctg gagatcgagg accggaactt
     3361 cgaccatgac ctctccctgg aggagaaggt cgccctcctc tcccgggagg cagtcttcat
     3421 ccgctccgag ttcgggtgat tcgctacctt tatttcatcg ctctccccac ttctttcggt
     3481 agacgggagg gggaggcacc ggggcgtgcc gtttcccggt tcccgcgtga aaacccatgg
     3541 cttcagagat cgagctcctg gcattttttc tctgcatcat cctctccgcc ttcttctccg
     3601 gctccgaggt agccctcctc tccatcaccc atgcaaaggt gaggaccctc ctcgcccggg
     3661 gaggcgggag cgcccaggcc ctcgccgact taaaaaggat gcccgaccat atcatcatca
     3721 cggtcctcat cgggaacacc ctggtgaacg tcggggcggc ctccattgcc acctccctcg
     3781 ccatcgagcg gttcggggac acgggcatcg ggatcgcgac cggcatcgtg gtcctcgtcc
     3841 tcctcatctt cggtgagatc gggccgaaga tgctcgcggt ccggttcacc gagcccgtcg
     3901 ccctcggggt gtcccgcccg atccggttcc tcgcgacggt cttctccccc ttcatctggg
     3961 tcttcgaccg gctggcccgc tccatggagc tcacgaaggc ctttgcgaag ccggccatca
     4021 ccgaggagga gctccggggc tggatcgagc tcggccagga ggaggggacc atcgagaagg
     4081 gggagcagca gatgctcttc aacgtcctcg agttcggtga caccacggcc cgggaggtga
     4141 tgacaccccg ggtgagcgtc accatgatcg atgagcatga ttctctcacc caggccgtgt
     4201 ccatcttcaa ggacaccggt ttctcccggc tcccggtctt ttccggtgaa aaggggaacg
     4261 tcgtcggcct cctgaacgtg aaggaggtct tccccgtggt tctcgaggag aagcaggggg
     4321 tgggcatcgg ggatctcatg cacgaaccct tcttcgcccc cgagacggcg aagatcgacg
     4381 atgtcctcaa ggagctccag aagaggaagg tccacatggg ctttgttctc gacgaatacg
     4441 gggagttcac cggggtcatc accgtggagg acatcctgga ggagctggtc ggggagatcc
     4501 tcgacgagtt cgaccaggac gagccggagg tgcaggtggt ggagccgggg gtgtacctcg
     4561 tggatggcca ggtgcgggtg aaggacgcga acgaccggct ggggatctcc cttcccctgg
     4621 agacatccta cgagaccctc gggggcctcg tcatcgaccg tctcgggcac atcccccggc
     4681 ggggggagac ggtctccctc gacgggacgg ccctcaccct cacgatcatc cagatgctcg
     4741 ggcggcgggt cggcacggtg aaggtgaccg tatcgccacc aatacatggg gagggctgaa
     4801 gcatgggccg ggcatcagca gctccgaagc ggatcgcggt cctcgcctcg ggaaggggct
     4861 cgaacttcca ggccctcatc gatgccgtcc gggcgggatc gattcccgcg accatcgtcc
     4921 ggctcattac cgataacccg gaggcccatg ccatccaccg ggcaggagag gcgggaatac
     4981 cggtctcggt cctcgccttc gggaggttcc cggggaagga ggcctacgag gccgccctga
     5041 aacgcgagat ggaggattgc ggtgccgacc tctacgtcct cgccgggtac atgcggatcg
     5101 tcccgggcgc cattgtgaag gcatttccgg gccggatcat gaacattcac ccggcccttc
     5161 tcccctcctt ccccggtctc cacgcccagc ggcaggcact cgtttacggg gtgaaggtgt
     5221 cgggatgcac cgtccacttc gtcgacgagg ggaccgacac gggccccatt atcctccagc
     5281 ggtgtgtaaa ggtcgcagcg ggagacgacg aggagagtct ctcggcccgg atcctcgtgg
     5341 aggagcaccg gatcctcgcc gaggcagtcc ggctcttctg cgaggggaaa atccggctcg
     5401 cgggccgaaa ggtccggatc gggtgaagcc ccggggcctg aatcccgcgg gacacccgga
     5461 accgatatag ggcccgcgga caaaggatgg gatggcatgg gaaggcgatc gcagaccccg
     5521 gaatcgagaa ggctggcacg ggagcggatc gagattctct tcgaccgggc ccgggagttc
     5581 tttcccgaga atcccgccct ttcggaccgg tgcgtcgacc tggcgaggcg gatcgcgatg
     5641 cgccagaggg tgaggatccc ccgggagctc cggaggcggt tctgccggaa gtgctcggct
     5701 tacctcgtcc ctgggtctac agcccgggtg agaatccgaa gggggaagat gatcatgacc
     5761 tgccttcggt gcagcgggca ccggaggttc cggctggggg gccgggaagg gatccgggcc
     5821 cgtgcagcgg tcgagagggg atccgggacc gaaaggtcct caggggatcc ggggcccgcg
     5881 ggggtcacaa agggatccgg gaccgggggg tcgacgtgac ggatacatcc ccgatgcaga
     5941 ccctgaagcc cacggtctgg atcgggaaaa aagggggcac tcccgaggcg atggccgaga
     6001 tccggcggca gctcgaggca aagaaaccgg tgaaggtgcg cttcctccgg ggggcggaga
     6061 tggacccgga ggccctcgcc gcggagacgg gcggggagat cctcggggtc cgggggagaa
     6121 ccgtggtcct gagaaaggaa aggtagccat gtcgggaaat gcccttcagg ttatttccct
     6181 cccgctcgga tggttgatac cattctccgc gtctcgaaaa gatataaaaa cgtatgccgc
     6241 gaatatgtaa agactgcgat agtacgcggg gacgatgggt gcaccatgac aacgctctat
     6301 gacatcccgg cagggctcct catcccgagg ctcgccgagg agctgaagaa gaggaaggag
     6361 atccagccgc cggcatgggc ggcctttgcc aaggcagggg tccaccagca gatgccgccg
     6421 gaggaccccg actggtggtt catccgggcc gcctcggtgc tccggaggct ctacatcgac
     6481 gggcccgtcg gggtccagcg gatgcggacg gcgtacgggg gaaagaggga ccggggctcg
     6541 agccccttcc agttccggaa gggcggcggg tcctcgctcc ggaaggtcct ccagcagctc
     6601 gaggccgcgg gcctcgtcgc aaaggacaag accgggagga gcctgtcggt cgagggaagg
     6661 aagttcctcg acgggatcgc ccacggcctc aggccggtgg cccaggagaa ggccgagggg
     6721 ctctccaagt actaggcgtg gtggccgatg ggtgacgacg agctcgccga gctccggagg
     6781 aggaggctcg agcagctcca gcgccagtcc gctgaccagc aggtcctgga agagcaggcc
     6841 aggcgcgagc agcaggtgga ggcgcagatc caccacatcc tcctccagat cctcgagccc
     6901 gaggcccgag agcgcctggc gaatatcaaa ctcacgaagc ccgatttcgc ccgggccatc
     6961 gagcagcagc tcgtcgccct cgcccagagc gggcgcctca agcagaagat caccgacgcc
     7021 cagctgaagg agcttctccg tcagctcgtc ccccagaaga aggactggaa tatcaggagg
     7081 gcctgaccgg aatcggcccg gccccgttcc cgagatacaa ggagatatga acatgagcag
     7141 agtcacgaaa ggtctcaaga tccggctcgc caaagcctgt gaacagaacc gccgcgtacc
     7201 cgcctgggtt atgatgaaga cgaagcgggc ggtgtcgacc cacccgaagc gccgcacctg
     7261 gcggcgctcc accctgaagg tgtgagccat ggtcgagaag ctgaaggagc agatctacat
     7321 catccccctc agggatgtgc agcacacgcc ccggtgggag cgggggaagc gggccatgaa
     7381 ggccgtccac gactatcttg cccggcatat gaagagcgag gacatcaaga tcgaccagag
     7441 catcaacgag gcggtctggg agcgggggag ccacaagccc ccttcccgga tccgggtccg
     7501 ggccatgaag ttcgaggacg ggcaggtcca ggcggagctc gcgaaggagt gagatgagtt
     7561 ccaccatcgc ctttgccggc gaccctcaca tcggcgtctt cgtccgggtc ctcgaggacc
     7621 tcgtgctcct cgcgcccctg gcccccgagg gcttccgggc ggccatcgag gaggagctcg
     7681 aggccgaggt ggtggagacc accatccagg actcggccat cgtgggggcc ctcgccgcag
     7741 ggaaccggcg gggggtggtg gtctcggggc tcgcctcgag ggacgaggtg gcccggatcg
     7801 aggagcaccg ggaggtgatg gtcctcgggg acgggatgaa cgccgcgggg aacgtcatcc
     7861 tggcaaacga cgtgttcgcg gcggtgaacc ccgagatgcc cctcgatatt gccgaggaga
     7921 ttggctcgtt cctccgggta ccggtgctcc ggctctcctt cgccggctac cgggcggtgg
     7981 ggaaggtggc ggtggccacg aaccggggga tcctcgtcca cccccgggcg aaccccgcgg
     8041 agatcgccgc cctcgagcag gcggtggacc tccccatcgg agtcgggacc gtgaacctgg
     8101 ggagcggtct cgtggggagc gggctcatcg cgaacagccg ggggtatctc gccggggtcg
     8161 ggacgagcgg ctttgagctg ggtcgaatcg aagaggtact gggatttctg gagtgaaggg
     8221 agatgtcgtc acagagttac cgggtggaag gaaccgcgag gatcgacggg gaatggaggc
     8281 cgtaccggaa ggtcatcagc gccccgaacg agaaacaggc gagggagagg gtctacaccc
     8341 tcctcgggtc gaagcaccgc ctgccgaggc gggagatccg gatcgggaag atcgagccgg
     8401 ccgagggtga gtgagatggc cgagccggag gccgatctcc agaccctcca gtactacctg
     8461 aacgagtaca gccagcaggc cgagatcttc tcccggcagc tcgagatgat cgagcagaag
     8521 cggatcgagt ccctcgcggc catcgagacc atgaaggccc tcgttgccga ccgggacgcg
     8581 cccaccctcc tccccctcgg cggcgggacc atggtgcggg tgaaggtggg cgatcccgag
     8641 aaggtcctcg tgaacgtcgg atccgacgtc atcgtccagc ggacgaacgg ggaggccatc
     8701 acctttctcc aggagaagat caccgagatg gaggccatgg agaagaaggt ggcccagacc
     8761 atcgaccagc tccgggcgca gatgtccggg gtggcccggc agatcgagtc ggcctacgcc
     8821 gcgcaggaga gaccccaggg atagggcatg ttcgacggcc tgagggagaa gctccgctcg
     8881 gtccaggagg tcttcgggac gagcatccgg gccgcctccc cctctccggc cggagagacc
     8941 ccggaggcga aggccgggcc gggcctcgcc gagcgggtga aaatccttgt caccgacggt
     9001 gagctcgtcc tctccgagaa gcagatagag ggacccctcc aggagctcga aatggttctc
     9061 ctggaaaacg acgtggcgct cccggtgacc gacgaaattc tccggcgggt gaaggaggac
     9121 ctcgtcggga aacaccggcg gatcggcgag tcgccggagg aggttatcct ctcggccctc
     9181 cgggacgcca tcgtccgggt cctcggggag ggcttcgacc tcgcggccta catcaggggg
     9241 cgggagaggc cggtgaagat cctcttcacc ggcgtgaacg gcacggggaa gaccaccacc
     9301 gtggccaagg tgggggacta cttaaagaga ctcggattct cggtggtcat cgcttccggt
     9361 gacaccttcc gggcgggggc ccttgagcag atcgcgaccc atgccgagag gctcgggatc
     9421 aaggtcatcc agcacaaggc aggcggggat ccctcggcgg tccttttcga cgccgtggag
     9481 tacgcgaagg cccaccggat ggacgtcgtc ctcgccgact cggcgggtcg gttccacaac
     9541 cgggtgaacc tgatgaacca gctcgagaag atgaagcggg tgatgagccc ggacctcgtg
     9601 gtctacgtcg acgaggcggt cgccgggaac gacgcggtgg tccgggccga ggagttcgag
     9661 cgtctcgtcg ggacagacgc cgtgatcctc acgaaggcgg acatggacat gcggggcggg
     9721 gcggccatct ctattgccca caccgtggga aagcccatca tgttcctcgg cacgggccag
     9781 gggtacgacg atctcatgcc cttcaccccc ggtggcctcg tggacgagct cctcgggggt
     9841 gcctgatgct cgacaagctc tcctcctccc tcaaggacgc cctgaagaag ctcgcgggga
     9901 aggcggtggt ggaccgggcc gcggtggagg aattgatacg ggacctccag cgggccctca
     9961 tccaggccga tgtgaacgtg cggctcgtcc agggcctctc ccaggcggtg aagacccggt
    10021 ctctcgacga gcagcctccg aaggggatgt ccgtccgcga gcatgtcctc cgcatcgtgt
    10081 accaggagct cctccggctc atgggcgcac gggccgaggt cgccctcggc ccccagagga
    10141 tcctcctcgc cggtctccag ggaagcggga agacgacgac gaccgcgaag ctcgcccgct
    10201 acttccagcg gaagggcatg aaggcagggg tcatctgcgc cgatactttc cggccggggg
    10261 cctatgacca gctcgccacc ctctgcgggc agtcgaacat cccctgctac ggggagccgg
    10321 cggagaaggt cgccgagggg atcgtggagc ggggcctcgg ctccctcgcg gcctccgagg
    10381 tcatcctcat cgacacccag gggcgccacg ccctggagaa ggacctcatc gaggagatca
    10441 tccgcctgaa cgccatcagc cgggccgacc accgctggct tatcatcgat gccgccctgg
    10501 gccagcaggc gagggaccag gcgaggaggt ttcacgaggc catcggcatc gacggtgtca
    10561 tcgtgacgaa gatggacggg accgcgaagg ggggcggggc cctctccgcg gtctcggaga
    10621 cccagagcgg gatcgtcttc atcgggagcg gagagaccat cgatgacctc gagcgcttcg
    10681 acccggacag cttcatctcc cggctcctcg ggatggggga cctcgcggcc ctcgcggagc
    10741 gggccgagga ggccatcagc accgaggaaa tggacgtcca gggcatgctc cgggggaagt
    10801 tcaccctccg ggacatgtac aagcagctcg acgccgtgag ccggatgggg cccctcaagc
    10861 agatcatgtc catgctcccc ctcggcggga cggatgtgcc ctccgaggcc atcgacgtca
    10921 catcctcgaa gatggcccgg taccgggtga tcatggactc catgaccgag caggagctgg
    10981 acgacccgaa cgtcatcagc ggctcgagga tccaccggat cgccgccggg gccggttcct
    11041 cgccggacga ggtccgggag ctcctgaagt actaccggct catgcagcgg gccctgaagg
    11101 ggatgagggg tggaaagttc aacctccagc gcctcatgag gcgcttctcc gggatgtgag
    11161 atgcgaagct ttgcggtggt ggggcatctc gcgcccacgg gagacaggtt cacgctggac
    11221 gacctacccg ggtcggcggg gaggctggac attctctgcc ggtgcgtgaa cgcggccctc
    11281 tccctctccc acgggatccg cagggatgcc gggatccacc tgatcctcct cggtcccccg
    11341 gacccgcccc gggacgtcct catcagcggg gcatcggttc gttccctttc cccggacgag
    11401 aggagcacag ccgccctgat ccggaaggcc ctcgccctgc ccgtcgggga ggccttccgg
    11461 gagtcgggtc ccggtatctc ggtccggcgc ggaaatctcg cagggctcgt cccgggcggc
    11521 ggatacacgg tcctctcgga gaccggagag gatgtgaggg gggtaaagaa tcttcccgcc
    11581 ggcttcatcc tctcggacca cctggatttc actccggagg aggaggcgat cctcgcgggg
    11641 ctcccgcgca tctcggtggg gccgagggtt ctccacgccg atcatgccat cacggtcctc
    11701 cagaacgagc ttgaccgtcg ggaggcggga tggagataga ggagcaggtg gaccggatcc
    11761 tcgcgtacgg ggagatctgc gaccactgcc tgggccggtt cttcggcaag aagtcccacg
    11821 gactcgcgaa cgaggagcgg ggccacgggc tccgggtcgc ccgcgccctc agggtgaaca
    11881 cccccttcgt tcccccgtcg gggccctgct ggatctgcca cgacctcttc gccgggatcg
    11941 ggacatgggc ggaaagggtt gtccaggccc tctcgggaat cgagtactcg accttcctta
    12001 tcgggacccg ggtgcctccc ctcatggccg agagcgagga gcttgtctgg tcggatctcg
    12061 gcctcgacgg gcccgagccc ctgaagtccg agatgaaccg cgaggtaggg aaggttgtgt
    12121 cggccatcac cggcagggcc gcggacctcg cccggcccga tgtcgtcgcc atcctcgacc
    12181 tcgcccgcga aaccgtcgag ctcgagatct cccccctctt cttccggggc cggtaccgga
    12241 agctcgagcg ggggatcccc cagacccggt ggccctgccg ggagtgccac ggggccggct
    12301 gcgagcggtg cggctttacc gggaagatgt accccgactc ggtggaggag ctcgtcggga
    12361 ggacggcggc gggggtattc cgggccgacg atgcggttct ccatggcgcg ggaagggagg
    12421 acatcgatgc ccggatgctc ggcaccggga ggcccttcgt catggagatc gtggcgcccc
    12481 ggcggcgcgt ggtggatttg gcggccctcg aggaaagggt gaacaccgcc gccgcgggcc
    12541 gggtcgggat caccatcgag ggctggtcca cgaggggcga ggtggaaacc cttaaatcag
    12601 aaaaggcgca taaaaagtac aggatctccg ttgagattga gggcggaatc gcccgaaagg
    12661 aactcgaaga ggccctggat cgcctgaagg gggccgtggt ctcgcagcag accccggccc
    12721 gggtggtcca ccggagggca gacaggatac gggaacgccg ggtcgtcgat gcgagggtgg
    12781 caggccagga aggcggcaca tttttccttg atgtcgtcgg agaggcaggc ctgtacgtga
    12841 aggagctcgt atcgggcgac ggcgggagga cccggccgag ccttgccggc ctcgtcggga
    12901 ggccggcccg ggtcgtccag ctcgacgtcc tcgaggtcga gggaagcgag gagagggagt
    12961 aaacaatggc acaccatcac ggtccacgga aaaagacacg ctacaagttc aagaaggaga
    13021 tacggcgccg ggggatcccc ccggtcatga ccgccatcca gaagttcgag gtcgggcaga
    13081 aggtccatat cgtctgcgaa ccgagcgtcc acgaggggat gcccaaccgg cggttccacg
    13141 gaatgaccgg gaccatcgtc ggccagcggg gccgggcctg gctcctccag atccgggacg
    13201 gggaaagtac aaagaccgtc atcgcccgac cacaacatct aaaagcccaa aggtgaagta
    13261 tccaagaggt acgctcacga tgcgtgtaaa agcggtactc ggagaggaga gggtcaccct
    13321 cgcggaactg aaggagaccc tcaccacggt ggaggagtcc cggcgggagg acgctaccga
    13381 gatggcctac gagctccggc gctccatcga gcatgcgacc cacctcacga agaccacgcc
    13441 gaagcaggcg aaggccctcc tttcggccct cctcgccctc gagaagatga agcccgacat
    13501 cgcctaccgg attacaaaca tcatgcccag gacgagggat gagctccggg ccatctacgc
    13561 gaaggagcgg ttcaccctca cgggcgagga gctcgaccag atcctctccc tcgtctccga
    13621 gcatctgtag ggtgaccccg atgaggaccg agaagaagga gaactacgcg atagtgctgg
    13681 acatccttca gaaggggcat gccgacgacc cgcgcccggt gtacaagcgg gagcccctgg
    13741 tccaggccct cggtatcgag cagttcaaga tcctcgagct cgtcccgaag ccgaacgccg
    13801 tcatccagat ccacgagaag ctgtatatcg gtgacgagga gcgggagaag atcgagcggg
    13861 tgaagcggcg gatcggcttt gaggaactga cccccaccgc ccgggtcgag gtcccgtacg
    13921 tcgtcgagca gatcgtgaag gagcacgagg accggttcgt ccagttcttc aacacctcgg
    13981 tccccgtcac cccgaagctc cacatgctcc acctccttcc cgggatcggg aagaagctcc
    14041 tctgggagat cctcgacgag cgccagaggc agcccttcat gtccttcgcc gacatcgccc
    14101 agcggatcaa ggccatgccc caccccgacc ggatggtcat caaccgggtg ctcgaggaga
    14161 tcgaggaccc cgagaccaag taccgggtct tcaccgcgcg atgaccgccc ggagggacca
    14221 gcacttcctc gtggacgagc gggccgtgga gcgcatcctc tccctcgtca acgtcgcggg
    14281 ccataccgtc cttgaggtcg ggccggggaa ggggatcctc acgaggggcc tcctcgaccg
    14341 gggtgccgag gtcgtggcgg tggaggtcga ccccttcctc gtcgaagagc tgagggggac
    14401 cttcgccccc gagatcgccg atggatccct ccacctcatc gaaggggatg cggtgaaggt
    14461 ggagcttcct cccttcgagc tcgtggtttc gaacctcccc tattccatct cctcccccat
    14521 taccttccgc ctcctggaga ggggattctc cgaggcgatc ctgatgtacc agtccgagtt
    14581 cgccgaccgc atggtcgcga agatcggtac tcccgcatgc gggcgactca cggtcatggt
    14641 ccagacctat gccgaggtga ggaaatgcat ccacatcccc ccgaacgcct tctcccccag
    14701 gccccaggtc cactccaccg tgatcaggat ggtcccccgg gagccgccct atcccaccgg
    14761 ggaccggagg cactatgccg acgtcgtgcg ggtcctcttc acccagcgga ggaagacggt
    14821 gaggaacggc ctccggtcgg ggaaggggat cttcggggcc gaccgggtgg agcggatgat
    14881 cgccgccact ccgccggagg tcctcggcct gcggcccgag gccctctccc tccgggactt
    14941 tgcggccatc gccagagtgg aatagatgga ggccgatccc gatcaggtct acccggtccg
    15001 ggaggacacc ctcctcctcc tcgaggcggc ccttgccgag gcgggaccgg cggacagggt
    15061 cctggagctg ggaacgggga gcgggtatat cgccatgcgc ctggcgggga gggtctcctt
    15121 tatcgttgcc accgatctga atccccacgc ctgccggctg gcatcggccc aggggctcgg
    15181 caccgtcagg gcagacctca ccgcgggcat cagggggtcc ttcgacctca tccttttcaa
    15241 cccgccgtac ctgcctacga aacccgggga acggctggac gactggctcg agaaggccct
    15301 ggacggggga gagagcggcc gggatgtcat cgcgaacctc atcccggatc tcccccggat
    15361 cctcgcgccc gggggaaggc ttctcctcgt catctccgag gtaacaggcg tacctgaagt
    15421 cctcgacctg gtcacccggg cggggttctc tcttgaaatc gcgagaaggg tacccatcga
    15481 aggggaggat ctcatggttc tgaaggcgat ccgccgacgg gattgaacgg ggtgaaccga
    15541 gggtcccgcc acgcccacaa cctatttccc tcccccttac caaccattcc ttcgtgagcc
    15601 cgagggaaag aaggattccg gaggatgctc aggcggtcat cgatcatgcc gtctccggta
    15661 tcccgaaggg aaaggtcatc gccatcttcc tctacggctc ggtcgcccgg ggggaggaga
    15721 agcccctctc ggacatcgac ctctgtgtca tcaccggccg gggaatctcc cgggccgaga
    15781 gggaacggat cctggtccac gcctccccga ccctcgacat caccctcttc tgggacctcc
    15841 cccttcccat ccggttccgg gttctcaggg aggggaggct cctctggggg gaggaggacc
    15901 tggagctcca ccggatccgg cgggggacgc tccggtgtta ccgggagata gggcggttga
    15961 tcaggcgcca cgaacggatg atgctcgatg cataagccgt acgaccgcga gcaggtgacg
    16021 aagatcatct cggacatcga gaggtacacg aaggacctgc aggagctcgg catctcgggc
    16081 agggaggagc tcgaggaccg ccgcaccctc tacgccctct cgatgctcct cttcaccatc
    16141 atcaaccgcg ccctcgacct cgccgacgag gtgatcatca cgaaccggct cggcatgccc
    16201 cagtcgtacc gggagatctt cgacatcctc cggcggagac ggttcatcga ccgggaactc
    16261 gccgaggaga tggcggccct cgtcttctac cggaacatga tcgcccacca gtacgacgac
    16321 ctcaccgacg atgacctcta ctccctcttc cagcggctcg ggaccatcac gaagttcgtg
    16381 gaccggatga aggagatcat ccgggagggg ccgcaggcca gggggaagaa gcggtaggga
    16441 attcctgccc cccggaccct tcccgaaact acaataccct ttcccgtcca ttgatctccg
    16501 ggatccgagc atgggagcga cctacgtcga gaagatcttc tcccgcgggt gcaggcggga
    16561 ggcccgcgcc ggggaggtgg tgatggcgcc ggtggatgcc gccatgatcc atgacatcac
    16621 cgggcccctc gccatccaga agttccggga gatgggcggg aaacgggtct ttgacccggc
    16681 aaaggtcatc atgctcttcg accaccaggt gccggccgat tccatccccg cggcccagaa
    16741 ccacatcttc atgcggatgt ttgccgagga gcaggggatc gggaacctcg acctgaacga
    16801 gggggtctgc caccaggtcg tcgaggagcg gggcctcgcc gcaccggggg agatcgtggt
    16861 cggtgccgac tcccacacct gcatgtacgg cgcgacgggg gcctttgcga cggggatcgg
    16921 ctccacggac atgggctttg tcctcaaatt cggtgccctg tacttccggg tcccggagac
    16981 catccgggtg gaggtcagcg gggtctttcc aaaccgggtc gggccgaagg acctcatcct
    17041 cgccatcacg ggggatatca gggccgacgg ggccacgtac cgggccctcg agtttaccgg
    17101 ggagaccatg gggggaatgg ggatggcagg aaggatgacc tgctgcaaca tggccgtcga
    17161 gatgggggca aaggcgggca tcgttccccc cgataaggtc acctgggagt atatgaaggg
    17221 gaggagaagg atcaagccct tcgaccttgc cgccgacccg gatgcgtcct acctcgagga
    17281 gcgctcctgg gacgtttcgg atcttgcccc gaaggtggca gttccccaca acgtggatca
    17341 cgtcgtggat gtcggggagg tagccgggac ccacgtcgac caggtcttca tcgggtcctg
    17401 cacgaacggc cggttcgagg acctgaagga ggtggccgat gtcatgggcg accggaagtt
    17461 ctcgaagggt ctccgggtga tcgtcatccc ggcatcgcga acggagtacc tgagagctct
    17521 ccgggcggga ttcatcgaac gcttcgtgga ggcgggggca ctcgtcgagg cgccctgctg
    17581 cgggccctgc atgggcgggg ccttcgggct cctcgggccg ggcgaggtct ccctctccac
    17641 ctcgaacagg aacttcaagg ggaggcaggg gtcggcggag gcgaaggtgt acctcgcctc
    17701 gccatccacc gcggcggcga cggccatcat gggggagatt accgacccga gggaggtgtg
    17761 accttggtac gaaggacaag gaggggaggg ggacagctga aggaggcacc ccgggcgatg
    17821 tccgcgggcc gggtctggcg gttcggggat gacgtggaca ccgatgccat catcccgggc
    17881 cgcttcctca ccctctacga tccccgggag ctcgcaaagc atgccttcga ggggacgagg
    17941 gatgagttcg ccggcggggc gagggagggg gacctcatcg tcgcggggag gaacttcggc
    18001 tgcgggtcgt cccgggaaca cgcccccctc gcgctgaagg gggccgggat ccgggtcatc
    18061 gtcgccgagt cctttgcccg catcttttac cggaacgccg tcaactccgg cctgctcccc
    18121 ctcgtctgca cgagagcccg tgacatccgg gacggggagg aggccactct ccacctggag
    18181 gcagggaccc tcaccgtcgg cggaaggcgg ttcacggtcg aaccggtgcc ggacttcctc
    18241 cgggagatcg tggatgccgg cgggctcgtc gagtttgccc gggggctctc ggaggtggtg
    18301 acatgccgcg acatcacgac gtagccgtgg ttccggggga tggcatcggc cccgagatca
    18361 tcgcagaggg acggaaggtc ctcgatgccg cgtcggagag gttcggcttc gacatccggt
    18421 ggcgggatct cgacatcggg gcagagcgat acctgcggac gggtgaactc ctcacggaga
    18481 aggatctcgc caccctcggg acgtgccggg ccatctactt cggcgccatc ggagacgagc
    18541 gggtgaaacc gggggtcctc gagaagggga tcctcctcgc cctccggttc cacttcgacc
    18601 tgtacgtgaa cctccggccc gtgaagcttc tccccggggt ctggacgccc ctcgccgaca
    18661 agggcccggc ggatgtcgac ttcgtcgtcg tgcgggagaa caccgaggac ttttacgtcg
    18721 ggatcggctc ccggttccgg ggggaggaga ggaaagccct cgaggtgatc cggtccctgt
    18781 accgggtgaa gttcgggatc gatgccgaga gcgacgccga ggaggtggcc taccagatcg
    18841 gggtcgtcac ccgggaaggg acccggcggg tcatccggtt cgccttcgac gaggccctcc
    18901 ggaggggacg ggccgccccg aaggtcacct cggtggacaa ggcgaatgtc ctcaccgagg
    18961 tctacggcct ctggcgggag gtcttctccg aggtggcaaa ggggtacccg ggggtctcct
    19021 ccgagttctc cttcgtcgat gccatcacca tgtggttcgt gaagaacccc gagtccttcg
    19081 acgtggtggt ctcgccgaac atgttcgggg acatcatcac cgacctcggg gccatgatcc
    19141 agggcggcct ggggctcgcc ccgggcggaa acatcaaccc agacgggaca tccatgttcg
    19201 agcccatcca cggctcggcc cccaagtacc ggggcctcgg cgtggcgaac ccggtggcga
    19261 ccatctgggc cgggtcgatg ctccttgacc acctcggcga gcacgaggcg gccgccgccg
    19321 tcctcgacgg gatcgggaag gccatcgggg ccggggtgaa gacgaaggac atgggcggcc
    19381 ggtcccggac cgccgaggtc ggggactcca tcgcggcata cctgggaaac ctctgaaacg
    19441 gtcggagaag gttcggggtg ccccctcctg cctcacggga tgggggaaaa tcctacgaat
    19501 ttattatccc ggagaaggag ctctatggtg tcccacggac ggagtgtccc gactgaaaag
    19561 gtgaatggcg atgactgaag cactcgtgaa ggacgtaagc gggctgatcg ggatctggca
    19621 gccccggaag gcctaccaca tctaccccac ctacctcgag gacctgaaga agttcctcca
    19681 ggagaggatg aaggccccgg gatccttttt ctcgggaaag cgggcgaaca tcgagttcgt
    19741 cgacgggaag ggaatgaata tcggcgtgaa gaggaaggcc ggttccgcgg tcgaggcggt
    19801 cgggatcgtg atgaagcgcg acctcatcac cctcgatgac ctgaagctcc tcatggatca
    19861 gatcaaccag gccgagcaga tgtacccgga gctcttcgtg atcctcatgg ggacccagac
    19921 cgccgatatg gagagcaagc tcgggacctt cctcgcgaac aaggacggga aggcgaagat
    19981 caccgttatc aagaaataaa ttcctttttt tcaggccatc ctggtaccgg aggagccact
    20041 atccctccgg ttccgatcca tgcaggcgtt caggacctcc tccatgaagg agagagagcc
    20101 ctcgatgccc gccagggccc gggaccggag gagcacccgg cccctcaggg gcggggtgat
    20161 ccctacgaag gccgcacccg ggcaggcaga tcgctcgaag gaggagccca cgatgagatc
    20221 cggcttctcg gcggaaagga actcgtgtat cccgcccagg tctgccatct ccctgaccgg
    20281 ttggggggag gggcggatac cgttcctcga cccggtaacg acaatttcgg cgtcgaggta
    20341 ttttttcagg ataccggagg cgaggccggc gtacgcccat ccaccgaaga ccgcggcccg
    20401 gggcgggtcg aagcgccgga ggtacttgtc ggcggcacgg ccgatccgtt cctctgcccg
    20461 ggccgcctcg gcaaggaccg ggccgatgtc gatacc
//
LOCUS       DATGDI010000161         3769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_336958, whole genome shotgun sequence.
ACCESSION   DATGDI010000161 DATGDI010000000
VERSION     DATGDI010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336958"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VKO45_06230"
     CDS             <1..432
                     /locus_tag="VKO45_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HMA05509.1"
                     /translation="EISIEGDAGNYLGAAYRGDWRGMRGGIIRVKGNAGSDMGYFMNG
                     GTIIVGGNADVHVATHAEGNGKIIIKGNAKSKVGGQMVGGEIYIFGDLEVPMPTFEYR
                     KDVELEVDGTKAKFALFEGDTAERHPKRKGETIFGKIYMKY"
     gene            789..2093
                     /gene="mcrB"
                     /locus_tag="VKO45_06235"
     CDS             789..2093
                     /gene="mcrB"
                     /locus_tag="VKO45_06235"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     beta"
                     /protein_id="HMA05510.1"
                     /translation="MAKYKESCDLYDDEGKLLKSNVTLDKVSPIVNPAIKKMINLTKR
                     TVAVNMAGIENGVKTGSFGKGQILGRGLNLSIAKDVDSLAAKIKDLVQVTDGDDTKVT
                     KVGGGKLLLVEVPMARLEAAATYDAASTAVAAAATQAILDQYKVDMFNGSVVKSAVWG
                     TYPQTMDMAGGNIVTILSIPQNNEGLGYALRNINANHVAMITHRNAMQTCALSCTFEQ
                     AGEFEMGMAIGDFERAQLLTYAFQGLNANNMVYDIVKANGKSGTIGTVVQSVVERALE
                     DKVIKAGKKGKYFQFYETKDPMMWNAYAAAGTMAATMVNCGAGRFAQAVSSTLLYFND
                     VLEHETGLPSSDYGRVMGTAVGFSFFSHSIYGGGGPGVFNGNHVVTRHAAGVAVPCVC
                     VACALDAGTQMFGPEQTSKVYQDTFGTIDEFARPLQAIAKGA"
     gene            2145..2588
                     /gene="mcrD"
                     /locus_tag="VKO45_06240"
     CDS             2145..2588
                     /gene="mcrD"
                     /locus_tag="VKO45_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase operon protein D"
                     /protein_id="HMA05511.1"
                     /translation="MPARFLYPETTEKLLNGICKVGVVRRMTLNGPRLPPTITEGPGR
                     GLPNPHPGRKVIHVGGEEFELQVQVGTIILELENPDAIPVIRQVCDEVFVNFSYTLQE
                     GKFMKSQMTTSDYAKYGVIEDERMLGMADPRAKGSRCPIILQGTK"
     gene            2590..3219
                     /gene="mcrC"
                     /locus_tag="VKO45_06245"
     CDS             2590..3219
                     /gene="mcrC"
                     /locus_tag="VKO45_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase I operon protein C"
                     /protein_id="HMA05512.1"
                     /translation="MSIGRITQVVDCRESIGMGKGGGIAQRGTISECRYPDVIVVAMS
                     PGHRHVTKPVCDITSALRREGAEFSVTTLVLSSGSGVPDDVPAVGGAVLGAYFTLNEK
                     EIRQIEQHKVAILHHGNVRSHVVHKVRKILEQCEIAAVVVSQVPIDYEDFAKAGVKTA
                     YVMPPADQIRTKGTVMAIVTGVTRGQTPTRTNIAEVIQAVMKVMRSQKR"
     gene            3231..>3769
                     /gene="mcrG"
                     /locus_tag="VKO45_06250"
     CDS             3231..>3769
                     /gene="mcrG"
                     /locus_tag="VKO45_06250"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     gamma"
                     /protein_id="HMA05513.1"
                     /translation="MAYKPQFCAGSTKIAENRRNFMNPAYKLQKIRDITDEDVVLVMG
                     HRAPGSAYPTTHPPLSEAGEPKCPIRKIVTPLDGAKTGDRVRYIQFADSYWFAPMHPY
                     LRSYMASIRYRGVDPGTLSGRQIVECRERDREKIAKELVSTECFDPARSGIRGCTVHG
                     HSLRLDEDGMMFDMLARRIL"
BASE COUNT          854 a         1128 c         1126 g          661 t
ORIGIN      
        1 gagatctcca tcgaagggga tgccgggaac tacctggggg ccgcctaccg cggcgactgg
       61 cgtggcatgc ggggcggcat catccgggtg aaggggaacg ccgggagcga catgggctac
      121 ttcatgaacg gcggtaccat catcgtcggc gggaacgccg atgtccacgt cgccacccat
      181 gccgaaggga acgggaagat catcatcaag ggcaatgcga agagcaaggt cgggggccag
      241 atggtcggcg gggagatcta catcttcggc gacctggagg tccccatgcc caccttcgag
      301 taccggaagg acgtggagct cgaggtagac gggacaaagg cgaagttcgc cctcttcgag
      361 ggggacacgg ccgagcgcca ccccaagcgg aagggagaga cgatcttcgg taagatctac
      421 atgaaatact gatccttttt ttccctgaaa cgcgcagcag ctgataaagg gtttttcggt
      481 acaatgccca aagatatcac aaaaggtgct acgatttata tgatctctca tactcgaaaa
      541 tttttgaacg ggttatataa acttggtatt taaggattat tctttcttta tttattattt
      601 taattctttt atataaaaaa attgggataa aatagcaatc cttatatggg ggtatcgttt
      661 agtatcgtat gtcccaagag ggacgtgcag ttagtgaccg tcactcacca gcagtgatcg
      721 cgcgaaggcg cacacacttc agttctctca atgtatagcc tactgaaagc tatctacgga
      781 ggaaatttat ggcaaaatac aaggaatcct gtgacctcta cgacgacgag ggcaagctcc
      841 tgaagagcaa tgtgacgctc gacaaggtca gccccatcgt caacccggcc atcaagaaga
      901 tgatcaacct caccaagagg acggtcgccg tcaacatggc agggatcgag aatggcgtga
      961 agaccggatc cttcggcaag gggcagatcc tcggccgggg cttgaacctc tccattgcga
     1021 aggacgtgga ctccctcgca gcgaagatca aggatctggt ccaggtcacc gacggtgacg
     1081 acaccaaggt cacgaaggtc ggaggcggga agctcctgct cgtggaggtg cccatggcac
     1141 gtctcgaggc agcggcaacc tacgacgcgg cctccaccgc ggtcgcggca gcggccaccc
     1201 aggccatcct cgaccagtac aaggtcgaca tgttcaatgg ctcggtcgtg aagtctgccg
     1261 tgtggggaac gtacccgcag accatggaca tggccggcgg taacatcgtc acgatcctct
     1321 ccatcccgca gaacaacgaa ggcctcggct atgcgctgag gaacatcaac gcgaaccacg
     1381 tcgcgatgat cacccaccgc aacgccatgc agacctgcgc cctctcctgc accttcgagc
     1441 aggccggcga gttcgaaatg ggcatggcta tcggggactt cgagcgggcc cagctcctga
     1501 cgtacgcctt ccagggcctg aacgccaaca acatggtcta cgacatcgtc aaggccaacg
     1561 gcaagtccgg aaccatcggt accgttgtcc agtccgttgt cgagcgtgcc cttgaggaca
     1621 aggtcatcaa ggcaggcaag aagggcaagt acttccagtt ctacgagacc aaggacccga
     1681 tgatgtggaa cgcctacgca gccgccggaa ccatggcagc caccatggtg aactgcggtg
     1741 ccgggcggtt cgcccaggct gtctcgtcga cactcctgta cttcaacgac gtcctcgagc
     1801 acgagaccgg gctcccgagc tccgactacg gccgtgtcat gggtaccgcc gtcgggttct
     1861 cgttcttcag ccactccatc tacggtggcg gcggccccgg tgtcttcaac gggaaccacg
     1921 tcgtgacccg gcacgcggcc ggcgtcgccg tgccctgcgt ctgcgtcgcc tgcgccctcg
     1981 atgcgggcac ccagatgttc ggacccgagc agacctccaa ggtctaccag gacaccttcg
     2041 gcaccatcga cgagttcgcg agacccctcc aggccatcgc gaagggagct taaagacgtt
     2101 cgtgaaccgc aatgactgaa gccatatttc ctcagatccg gataatgccg gcgcgtttcc
     2161 tctatcccga gacaacggag aagctcctga acgggatctg caaggtgggc gtggtcaggc
     2221 ggatgaccct gaacggtccc cgcctccctc ccaccattac cgaagggcca gggcgagggt
     2281 taccaaatcc ccacccgggc cggaaggtca tccacgtcgg gggcgaggag ttcgagctcc
     2341 aggtccaggt aggaactatc atcctcgagc tggaaaaccc ggacgccatt cccgtcatca
     2401 ggcaggtgtg cgacgaggta ttcgtcaatt tctcgtacac cctgcaggag ggcaagttca
     2461 tgaagtccca gatgacgacc tcggattatg cgaagtacgg tgtgattgag gacgagagaa
     2521 tgctcgggat ggcagacccc agggcaaagg ggagccggtg cccgatcatc ctccagggga
     2581 ccaagtgaca tgtcgatagg ccggatcaca caggtggtcg actgcaggga gagcatcggg
     2641 atggggaagg gcggcggtat cgcccagcgc ggaaccatct ccgagtgccg ctaccccgat
     2701 gtcatcgtgg tagccatgtc cccgggccac cggcacgtga caaaaccggt ctgcgacatc
     2761 acctccgccc tccgaagaga gggagcggag ttctcggtca ccaccctcgt cctcagttcc
     2821 gggagcggag ttcccgatga cgtacccgca gtaggaggcg ccgttctcgg cgcgtacttc
     2881 accctcaacg aaaaggagat ccgccagatc gaacagcaca aggtagcaat cctccaccac
     2941 gggaacgtgc gttcgcacgt cgtccacaag gtgaggaaga tcctcgaaca gtgcgaaatc
     3001 gccgcggtgg tcgtctccca ggtccccatc gattacgagg actttgccaa ggcaggggtg
     3061 aagacggcgt acgtgatgcc gccggcggat cagatccgga caaagggaac ggtcatggcc
     3121 atcgtcaccg gtgtgacgag gggccagaca cccacgagga ccaatatcgc cgaggtcatt
     3181 caggcggtca tgaaggtcat gagatcacag aaaaggtaag gtgaaagaaa atggcataca
     3241 aaccccagtt ctgcgcgggg tccacgaaga tcgccgagaa caggcgcaac ttcatgaacc
     3301 cggcgtacaa gctccagaag atccgggata tcaccgacga ggacgttgtc ctggttatgg
     3361 gacaccgcgc ccccggctcg gcgtacccga caacccaccc gcccctctca gaggccggtg
     3421 agccgaagtg cccgatccgg aagatcgtga cacccctcga tggcgcgaag acgggggaca
     3481 gggtccggta catccagttc gcagactcct actggtttgc gccgatgcac ccctacctcc
     3541 gctcgtacat ggcgtcgatc cgctaccgcg gtgtcgaccc gggtaccctc tccggccgtc
     3601 agatcgtgga gtgccgcgag cgggaccgcg aaaagatcgc gaaggagctc gtctcgaccg
     3661 agtgctttga cccggcacgc tccggtatcc ggggctgcac ggtgcacggg cactccctcc
     3721 ggctcgacga ggacggcatg atgtttgaca tgctcgcccg ccgcatcct
//
LOCUS       DATGDI010000162         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_220917, whole genome shotgun sequence.
ACCESSION   DATGDI010000162 DATGDI010000000
VERSION     DATGDI010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220917"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VKO45_06255"
     CDS             complement(<1..629)
                     /locus_tag="VKO45_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05514.1"
                     /translation="MKSGEVKKPGRKARGINEVVGFLLILAVVIMTITVYMIYFMPTL
                     GRDQEISQMSDVKERFAEFKLNIDSLWMTRQGTSSFGPSLSLGSGETTGLLSFFPFLR
                     PPRAGAVLALNQRDENITITSDSYILGGGGSKVSQAVGTSPVLLSVNTTPSNLYIKLY
                     ATDLLKERGVFVHGPAWDAWVNVTPNYIYTDPTSWTLNGTDITVTTLVGG"
     gene            complement(663..>2898)
                     /locus_tag="VKO45_06260"
     CDS             complement(663..>2898)
                     /locus_tag="VKO45_06260"
                     /inference="COORDINATES: protein motif:HMM:NF021607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HMA05515.1"
                     /translation="TGAQSDGDDIVFTDSGGTTVLPHEIESFTKSSGALVAWVKLPTV
                     SSSANTDIYLYYGNSSTASQQNASGVWDSNYRGVWHMNVTADSTSLNTCTNQGSTSTT
                     GKVNNARYFGTSQYIDCGAFENLNNQLTLETWLKLDTNIPASTWPNDVGKGNENWGGW
                     RLYSQNSNQLMFAYNGSDNSIHYDLYDNINLGTWYQVAITFDVSGNSARMYVNGQPYM
                     TAGASHGISNGPQNLIIGTTSNQLNTNLDEVRISNVARSPGWIATEYANQNRPDLFFS
                     VGTEEASPCGGSGGSGCTGIAVDSTASGASGNSTSTLSISHTTSGSNRLMLVGVSLQD
                     SDTSWNDYTNKVNVSSISYNGKSLSRVGYALNADDARVEIWRLIAPDTGTYNVIITLN
                     QSLHNNGYIVGGVMTFTGVDQTTPLGTFASQALNESVIPVNVTVSSAANELVFGAIAN
                     EYGSISSDTTGGQIGRWSRHVGIGMDAGDGNGLEYTNGAGSTKPGAASVKMNWTLGNW
                     YNHWAAAGVSIKPCSSGCTLGTVGVDVISSGQNTTPISGITLSHATSGSNRLMLVGVT
                     INNDNFEGVSTIAYNGTALTYVGYSNNLMSGNDDAYTEIWKLVAPDPGTHNVVITFNK
                     TLSRYASAGAMTFTGANQTIPLSTFAGNQGDDDNLINVTVPSAAGDLVFGVIADEYNS
                     VITNTDGGEIERWNYHLDSTDWTNGAGATKPGATPSVMLKWNLSSTVNHWATAGVSIK
                     PACV"
BASE COUNT          516 a          792 c          944 g          646 t
ORIGIN      
        1 ccgccgacga gggtggtcac ggtgatatcg gtgccgttca gggtccagga ggtggggtcg
       61 gtgtagatgt agttcggggt gacgttgacc caggcgtccc aggcggggcc gtggacgaac
      121 acgcccctct ccttcaggag atcggtcgcg tagagcttga tgtagaggtt cgagggcgtg
      181 gtgttcacgg agaggaggac gggagatgta ccgacggcct ggctcacctt ggatcccccg
      241 cccccgagga tataggaatc cgaggtgatg gtgatgttct cgtcccgctg gttgagggcg
      301 aggaccgcgc cggcccgggg cgggcggagg aaggggaaga agctgaggag gccggtggtc
      361 tcgccgctgc cgagggagag ggagggcccg aagctggagg tgccctgtcg ggtcatccag
      421 agggagtcga tgttcagctt gaactcggcg aagcgctcct tcacgtcgga catctgggag
      481 atctcctggt cccggcccag ggtgggcatg aagtagatca tatagacggt gatggtcatg
      541 atgacgacgg cgaggatgag gaggaagccc acgacctcgt tgataccgcg ggccttccgg
      601 cccggttttt tcacctctcc ggatttcatg cccgacaaac catcacaccc ggttgaccgg
      661 tgttagacac aggccggctt gatagacacc cctgctgttg cccaatggtt cacggtggag
      721 gacagattcc atttcagcat caccgatggg gtcgcacccg gcttcgtggc cccggcaccg
      781 ttggtccaat ccgttgaatc gaggtgatag ttccagcgct cgatttcgcc gccatccgta
      841 ttggtgatga ccgagttgta ttcgtcggct atgactccga agaccagatc gcctgccgcg
      901 gagggcaccg tgacattgat caggttatcg tcgtctccct ggttcccggc aaaggtgctg
      961 agggggatgg tctggttcgc gccggtgaag gtcattgccc cggcactcgc gtacctcgac
     1021 agggtcttat tgaaggtgat cacgacattg tgggtacccg gatccggagc gacgagcttc
     1081 cagatctcgg tataggcatc gtcattgccg ctcatgaggt tgttgctgta cccgacgtat
     1141 gtcaaggcgg tgccgttgta ggcgatggtg ctgacgcctt cgaaattatc attatttatc
     1201 gtaacaccga cgagcatgag gcggttggac ccggaggtgg cgtgggagag ggtgatgccc
     1261 gagatcgggg tggtgttctg gccgctggag atcacgtcca cccccacggt gccgagggtg
     1321 cagccgctgc tgcacggctt gatagagacg ccggctgctg cccaatgatt ataccagttc
     1381 ccgagggtcc agttcatttt caccgatgcg gcacccggct ttgtactccc ggcaccattg
     1441 gtgtattcaa gtccattacc gtctccggca tccatcccta tgccgacatg gcggctccag
     1501 cgtccgatct ggcctccggt ggtgtctgac gagattgaac cgtattcgtt cgcaatggcc
     1561 ccgaacacga gttcgttcgc cgcggaggag accgtcacgt tcacagggat gacactttcg
     1621 tttaaagcct gcgaggcgaa ggtccccagg ggggtcgtct gatccacacc cgtaaaggtc
     1681 atcacgccgc ccacgatgta cccgttgtta tgcagggact ggttcagggt gatgatgacg
     1741 ttgtaggtcc ctgtatccgg agcgatgagc ctccagatct cgaccctcgc atcgtcggca
     1801 ttcagggcgt aaccgaccct cgacagggat ttcccgttgt agctgatact gctgacattc
     1861 accttgttgg tataatcgtt ccaggatgta tcgctgtctt ggagggagac ccccacgagc
     1921 atcagccggt tggaaccgga ggtcgtgtgg gagatgctca gggtggaggt cgaattcccg
     1981 gaggctccgg aggcggtcga atccaccgcg atcccggtgc aacctgagcc gccgctgccg
     2041 ccacacgggg atgcctcctc tgtcccgacc gagaagaaga ggtcgggccg gttctggttt
     2101 gcgtactcgg tggcgatcca cccgggggag cgggcgacat tggatatgcg aacctcgtcg
     2161 aggttggtgt tgagctggtt cgaggtcgta ccgatgatca ggttttgcgg tccgttgctg
     2221 atgccgtggg atgcaccggc cgtcatgtag ggctggccgt tcacgtacat gcgtgcgctg
     2281 ttcccggaca catcgaaggt tatcgccacc tggtaccagg taccgagatt gatgttgtcg
     2341 tagaggtcgt agtgaatact gttgtcgctt ccattgtacg caaacatcag ctggttggaa
     2401 ttctgcgaat ataacctcca gccgccccag ttttcattcc ctttcccgac atcattcggc
     2461 catgtactcg cggggatgtt ggtatcgagc ttcagccatg tttcaagggt cagctgattg
     2521 ttgaggttct cgaaggcccc acaatcgatg tattgtgagg tcccgaagta ccgggcattg
     2581 ttgaccttcc ctgtggtgct ggttgaaccc tgattggtac aggtattgag ggaagtgctg
     2641 tcggcagtca cgttcatgtg ccagacaccc ctgtagttcg agtcccacac gccgcttgcg
     2701 ttctgctggc tggccgtgga agagttcccg tagtacaggt agatgtcggt gttcgccgag
     2761 gaggagacgg tcgggagctt cacccatgcg acgagggccc cgctcgactt cgtgaagctc
     2821 tcgatctcgt gggggagcac cgtggtcccg cccgagtcgg tgaagacgat gtcgtccccg
     2881 tcgctctggg cacccgtg
//
LOCUS       DATGDI010000163         3795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_278977, whole genome shotgun sequence.
ACCESSION   DATGDI010000163 DATGDI010000000
VERSION     DATGDI010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278977"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VKO45_06265"
     CDS             complement(<1..264)
                     /locus_tag="VKO45_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05516.1"
                     /translation="MILAALLQAVPFPAADGTPVINVPLLLFVFGVMSIVQSLVLGMK
                     KMAYATGYCIGAVAGMALFFGLFAGLLTDLFPQIVPRASTATAG"
     gene            complement(362..490)
                     /locus_tag="VKO45_06270"
     CDS             complement(362..490)
                     /locus_tag="VKO45_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05517.1"
                     /translation="MRDREEKEDRKPGISRRAVVLFGAMLGSYIAGRVYDLIKGRR"
     gene            complement(487..1239)
                     /locus_tag="VKO45_06275"
     CDS             complement(487..1239)
                     /locus_tag="VKO45_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMA05518.1"
                     /translation="METDPKKLSGKVAIVTGASSGIGRATARLLAGHGVKVALVSRSS
                     EKLENLAGELPGSLAVPADMTMIPQVKSMVKQTREHYGRIDILVNNAGQGYDAPVELI
                     DIGTLRYIYDLFITGPIIAMQQVIPIMRTQGGGTILNVSSAVALLDLPGMSPYASAKK
                     ALAGISLAARHELARDGIVVSVAYPYITLTEFEKNTIRAIPVPPDQMEPTGPYPPDSA
                     EFVAERLVEGILSGEAKIFSHEWQKSQGREER"
     gene            complement(1364..1531)
                     /locus_tag="VKO45_06280"
     CDS             complement(1364..1531)
                     /locus_tag="VKO45_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05519.1"
                     /translation="MNLVIACIGLFFSAEGAANILYWRTDNHPWYFQAGRFVRVALGV
                     VLITMAVAGIP"
     gene            complement(1574..1789)
                     /locus_tag="VKO45_06285"
     CDS             complement(1574..1789)
                     /locus_tag="VKO45_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HMA05520.1"
                     /translation="MRRRNWMLGFLGFLGFMGFQGIVGLAKGDWLEAVYVLWFSFFAW
                     FVNFLPEKGTQKVPDPGPPPSHPGTRE"
     gene            complement(1800..2603)
                     /locus_tag="VKO45_06290"
     CDS             complement(1800..2603)
                     /locus_tag="VKO45_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HMA05521.1"
                     /translation="MSAPPEPSLGAQKKLAVLIDADNTSASIIGALLDEIATYGIASV
                     KRAYGNWTSETLQPWKKTLLDYSIQPMQQFAYTTGKNATDSALIIDAMDLLYTEKFDG
                     FCIVSSDSDFTRLASRIRESGLTVYGFGERRTPKPFVQACDKFIYTDILGKPEPVAEP
                     KPGAHAEVRPPEKSRRPGEDKNLVNLLKAAAESCSDEDGWAYLADLGNNILQKKPDFD
                     PRNYGYKKLKDLVEDTRLFDIHESSVGDSPAKTIQVRFKKAKRKRGFRG"
     gene            complement(2652..2936)
                     /locus_tag="VKO45_06295"
     CDS             complement(2652..2936)
                     /locus_tag="VKO45_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05522.1"
                     /translation="MKARDKVKVGVVAAVCLMGLVVALKNVLHISPDIISRDMIIYII
                     VYFGFITALNFTEETPGEPASPKKTRDSPWTWSLLVILITLAIVAVYALG"
     gene            complement(3107..3610)
                     /locus_tag="VKO45_06300"
     CDS             complement(3107..3610)
                     /locus_tag="VKO45_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HMA05523.1"
                     /translation="MAGKILIAYTSRRGSTAEIAEAMGKELEAVGNSVQVTDMKNVAS
                     IEGFQAVVIGTPIYLGKVEKPVHAFVARHREGLLAVPVAAFAVGITPVNSPPEAVEGV
                     LATFRAALDPVKPVAVTMFAGVLDMSKMSFLERTIIGSMKILTGDFRDWDAIKKWARE
                     LPAVLKI"
BASE COUNT          705 a         1158 c         1176 g          756 t
ORIGIN      
        1 gcccgctgtc gccgtcgatg cccgggggac gatctgtgga aaaaggtcgg taagaaggcc
       61 ggcgaagaga ccgaagaaga gggccatccc cgccaccgca ccgatacagt accccgtcgc
      121 ataggccatc ttcttcatgc cgagaactag gctctgcacg atgctcatga ccccgaagac
      181 gaagaggagg aggggcacgt tgatcaccgg ggtcccgtcg gcggccggga aagggaccgc
      241 ctgcaggagg gcggccagga tcacgagaaa gaaggaaaac aggatctcga cgagagggag
      301 agtgatgccc tcggtaaacc cccggacaaa ggcccgtact cccccggtcc accctatcgt
      361 tctacctcct cccctttatc aaatcataca cccgccccgc gatgtacgat cccagcatcg
      421 ccccgaagag aacgaccgct ctccgtgaga tacctggttt gcggtcttcc ttttcttccc
      481 tgtccctcat cgctcctccc ttccctgact cttctgccac tcatgggaaa agattttcgc
      541 ctcgccggag aggatgccct cgacgaggcg ttccgcgacg aactccgcgg agtccggcgg
      601 gtacgggccg gtcggttcca tctggtcggg gggcaccggt atcgcacgga tggtgttctt
      661 ctcgaactcg gtcagggtga tgtacgggta cgccacggag acgacgatgc cgtccctcgc
      721 aagctcatgc cgtgcggcga ggctgatacc ggcaagcgcc ttcttcgccg atgcgtacgg
      781 gctcatgccc gggaggtcca gcagggcgac cgcggagctg acgttgagga tggtccctcc
      841 tccctgggtc ctcatgatgg ggatcacctg ctgcatggcg atgatcgggc cggtgatgaa
      901 aaggtcgtag atgtagcgga gggtcccgat gtcgatgagc tcgaccgggg catcgtatcc
      961 ctgcccggcg ttgttgacca ggatgtctat cctcccgtag tgctcccggg tctgtttcac
     1021 catgctcttc acctgcggga tcatggtcat gtcggcggga accgcgagcg acccgggaag
     1081 ttcccccgca aggttctcga gtttttcgct cgaccgggag acgagcgcga ccttcacccc
     1141 gtgacccgcg agaagccgtg cggtcgcacg cccgattccc gacgaggcgc cggtgacgat
     1201 ggcgaccttt cctgacaatt ttttcggatc cgtttccatg gggacctcac ccttcctcgg
     1261 atgagattcc ccgtgagcgc gggaggaaat aaagataccc gcgatgcaca attcttgaaa
     1321 tcccgggtga accgggtctc tccggaaccc cgccttcatc cggttacggg atgcctgcaa
     1381 cggccatggt gatgagcacg acgcccaggg ccacccggac gaaccggcct gcctggaaat
     1441 accaggggtg attgtcggtc cgccagtaga gaatattcgc agccccctct gccgagaaga
     1501 ataacccgat gcatgcgatt accaggttca tggttcttct cccggtgaaa cgatcatttt
     1561 ctcggccctg ttgtcactct cgggttcccg ggtgtgaagg cgggggaccg ggatcaggca
     1621 ccttctgggt ccccttctcc ggaagaaaat tgacgaacca tgcaaagaag gagaaccaga
     1681 ggacatacac ggcctcgagc cagtcgccct tcgcgaggcc gacgatcccc tggaacccca
     1741 tgaatcccag aaatcccagg aaccccagca tccagttcct tcgcctcacg cgaccccccc
     1801 taaccccgga agcccctctt cctcttcgcc ttcttgaacc ggacctggat ggtcttcgcc
     1861 ggcgaatcac cgactgagga ctcatggata tcgaagagcc tcgtatcctc gacgaggtcc
     1921 ttgagcttct tgtacccgta gttccggggg tcgaagtccg gcttcttctg gaggatgttg
     1981 ttcccgaggt ccgcgaggta cgcccagccg tcctcgtccg agcagctctc agccgctgcc
     2041 ttgaggaggt tcaccaggtt cttgtcctcc cccggtctcc gggacttctc ggggggcctc
     2101 acctctgcat gtgccccggg cttcggctcg gcgaccggtt cgggcttccc caggatgtcg
     2161 gtgtagatga acttgtcgca ggcctggacg aagggcttcg gggtcctccg ctcaccgaag
     2221 ccgtacacgg tgaggcccga ctcccggatc cgggatgcga gacgggtgaa gtcgctgtca
     2281 gaggagacga tgcagaaccc gtcaaacttc tccgtgtaga ggaggtccat ggcatcgatg
     2341 atgagggccg agtcggtggc gttcttcccc gtggtgtacg cgaactgctg catgggctgg
     2401 atggagtagt cgaggagcgt cttcttccag ggctggaggg tctcggaggt ccagttcccg
     2461 taggccctct tcacgctcgc gatgccgtag gtcgcgatct cgtcgagaag ggcaccgatg
     2521 atgctcgcgg aggtgttgtc ggcatcgatg aggacggcga gcttcttctg ggcgccgagg
     2581 gagggttcgg gtggagcact catatgggtg atccatcgcc cggcatcatg atatgggttt
     2641 tccctccggg gtcaaccgag ggcgtagacc gcgacgatgg ccagggttat caggatgacg
     2701 aggaggctcc aggtccatgg gctgtccctc gtctttttcg ggctggctgg ttccccgggg
     2761 gtttcctcgg tgaagttcag ggcggtgatg aagccaaagt acacgatgat gtagatgatc
     2821 atgtcgcggg agatgatatc cggggagatg tggagcacgt tcttcagggc gaccacgagc
     2881 cccatgaggc agacggcggc gacaaccccc accttcacct tgtccctggc tttcatgggg
     2941 agaagtggat tcgatccttc ttatctaggt tgtggacggg aacggcggta ttttttgcgc
     3001 ccctctcggg gaaaacgcgt cggcattgtg cttatcgttc tcgggagtcc ccaaacacgt
     3061 tactggcagt cgggaacgag gagacccatg ggaacaggaa aaaacttcag atcttcagca
     3121 ccgccggcag ctcgcgggcc cattttttga tggcgtccca gtcccggaaa tcgccggtca
     3181 ggatcttcat ggagccgatg atggttcgtt cgaggaagga catctttgac atgtccagca
     3241 cgccggcgaa catggtgacc gcgacgggct tcaccggatc gagggcggcc cggaaggtgg
     3301 cgagcacccc ctccacggct tcgggtggcg aattcaccgg cgtgatgccg acggcgaagg
     3361 cggcgaccgg gaccgcgagg agcccctccc ggtggcgtgc cacgaaggca tgtaccggct
     3421 tctcgacctt ccccaggtag atgggggtgc cgatgaccac ggcctggaac ccttcgatgg
     3481 aggcgacatt cttcatgtcg gtgacctgaa cgctgtttcc cactgcctcg agctctttcc
     3541 ccatcgcctc ggcgatctca gcggtcgatc ccctcctcga tgtatatgcg atgagtatct
     3601 ttccggccat ggtttctttc cctcacccta ttcctgtgcc aggagaaaaa ggatgaactc
     3661 ggatctccag ggctctacct catccacccg aacccgcggg cgatgaacac gagcccgatg
     3721 aggatgagga cggccgcgat gatgtacgtc caggggagga ccagtcctcc gacgatggag
     3781 aggcccacga ggacg
//
LOCUS       DATGDI010000164        11618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_17336, whole genome shotgun sequence.
ACCESSION   DATGDI010000164 DATGDI010000000
VERSION     DATGDI010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 11618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11618
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17336"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /gene="sepS"
                     /locus_tag="VKO45_06305"
     CDS             complement(<1..1448)
                     /gene="sepS"
                     /locus_tag="VKO45_06305"
                     /EC_number="6.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043816 - phosphoserine-tRNA(Cys) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-phosphoserine--tRNA ligase"
                     /protein_id="HMA05524.1"
                     /translation="MKFDAGKWRDRAREDFEKAWHEGPGVLTPPGGDGKYPRLRYGHA
                     RPHPVFDTVSRLREVYLSLGFEECCNPLIVDEQEVYRQFGPEAMAVLDRVFYLGGLPR
                     PNVGIGKERIDAIGEILGRPIGPDVETRLRETLHAYKKGEVEGDDLVHAISVVLAADD
                     ATVVKVMDRVFPEFRELAPESTRRTLRSHMTSGWFLTLSSNWQRRPHPIRLFSVDRCF
                     RREQEEGPTRLMTYHSASCVVAGEDVTNEEGKAVAEALLSAFGFTKFTFQPDEKRSKY
                     YMPGSQTEVYATHPVRGEVEVATFGVYSPSALAEYGIEVPVMNLGLGVERLAMILTGS
                     EDMRALSYPQFFPRDLTDREIAGAVSLKEEPATATGRFIARAIRETGMANPTAPGPCS
                     FTAWDGDLLGHRVKVFVEEPEANAKLLGPACGNEVFVHQGNILGIPDNEKWAQIRSEG
                     VSTGIVYIDAVAALAASRIEEAARCGKGTTVQV"
     gene            complement(1445..2350)
                     /gene="twy1"
                     /locus_tag="VKO45_06310"
     CDS             complement(1445..2350)
                     /gene="twy1"
                     /locus_tag="VKO45_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HMA05525.1"
                     /translation="MPSIPCEEPQRQLRRQGYQFVSPGSSAAVKPCLWCKRALCGGEM
                     CYKARFYGIGSHRCIQMTPTLRCTQRCLFCWRSFEHEPKTDTDIQPELIADRLLDLQK
                     RALSGYKPSYRVTPEKFREALEPRHVAISLAGEPTCYPHLPALVDILNSRGYTTFLVS
                     NGTRPWVLEETRPFQTYVSLCAPDRETYERLCRPVTDSWERVVESLGILGKRRSAIRI
                     TVVRGENDHRPDLYAGLIQDSGARFVEVKGYMFLGYSRKRLKKENMPEHGEIQAFASA
                     LAGGSDYEIRDESPASRVVLLERTG"
     gene            complement(2331..3992)
                     /gene="argS"
                     /locus_tag="VKO45_06315"
     CDS             complement(2331..3992)
                     /gene="argS"
                     /locus_tag="VKO45_06315"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HMA05526.1"
                     /translation="MFLDYSRRVAGAIREETGGADPTLTDGGIHADLASTIAFSLAKA
                     ERRSPAAIAGELAKRLSLRPDLAGIRIEAKGPYLNFIFGAEYLSDSIREAVKPGYGTL
                     PGREGRVVIEHTSANPNGPLHVGHIRNTIIGDCLARAFRKAGHRIETHYYVNDMGRQI
                     AIVVWAVDRYGPGDPGEKGDDRIARAYIQANRDLEGSPGSAPEIDGLMQKVEAGDPEV
                     MRKFRSAVTICLDGIKKTLSDLGVSHDRFIWESDFVRNGDVERVLGRIKRLAEYREDE
                     TVWLDLTAAGFEKPYILRRSDGTTVYAARDIAYHIWKARNFGRIIDVLGADHKLIGAQ
                     LVTTLGLLGETPPTIVHFEFVSLPEGSMSTRQGKFVSADALLAEVASRALDEVSSRRT
                     ELPESARRAIARSVAIAAIRYDMVKISPEKAMVFDWKEALDFERQSGPYIQYAHARAC
                     SILEKAGAFSPAFEYRDPMEIALVKAIARFPAVVEQVTLELRPHLLASYVHELANLWN
                     TFYQHVPVLKSEGVTRESRLTLVTATANTLSEALLTLGIDALDTM"
     gene            complement(3994..5283)
                     /gene="prf1"
                     /locus_tag="VKO45_06320"
     CDS             complement(3994..5283)
                     /gene="prf1"
                     /locus_tag="VKO45_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HMA05527.1"
                     /translation="MSAEKTVEAGQEQDEARRRYEFKKTLEKLEAKEGSGTELITLYI
                     PPDKQIHDVTAQLRDEYGQCSNIKSKQTRTNVQSAISSILARLKYYKRPPPNGMAVFC
                     GTVLLGGDRTDLECTIIEPPEPLNLYMYRCSSQFELDPLRSMLVEKQVYGLIVIDRRE
                     AYWGFLRGDRIEAVSGATSTVPGKQRKGGQSSVRFARLRLIAINEFFKKVGERASATF
                     LAEKEFFERFKGVLIGGPSPTKEEFEAGGFLHHEIQKRIIGLFDVSYTNESGLTELVD
                     AAQDALKGMDIVREKTIMNRFLQELVKENGNAAYGEESVRKNLEGGAVDTLILSSRLR
                     KLHVRIKCPNCTHRHETTVQTEAGVKIHEIELGGCPSCSTPLVIEEEVDIVEALTQLA
                     DRTGTKVEIISDEFEEGSMLFNAFGGIAAILRYRTGY"
     gene            complement(5569..6438)
                     /locus_tag="VKO45_06325"
     CDS             complement(5569..6438)
                     /locus_tag="VKO45_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMA05528.1"
                     /translation="MRSSSLLLGLIFAGLILGTGCLGTGSQGPTWSVTNDGYLGLTCP
                     GPATVNESILERNGSLTLREVSFRNIDEEVFGLLASPVNPRGAIVLAPGAGVTKERER
                     DRVETYAMAGCTALVLDLRGNGGETRGRPFDPNLDFMAFNLGAWPEFFKTACDISASR
                     EIIAGRWQVPVYAMGASNGGRYAAIAAAADPGFAGYIGVSTSGFGLAGNQYTGNASRF
                     LLSIDPDHAIGKISPRPVWIFHSREDPIINFTLGEELFSQAGNPKTFIVFNGTHGENE
                     EVDRRVMEICTSG"
     gene            complement(6438..6938)
                     /locus_tag="VKO45_06330"
     CDS             complement(6438..6938)
                     /locus_tag="VKO45_06330"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HMA05529.1"
                     /translation="MTDIYRGRRLRVVKELYRLPSGKEKERIVIHPGDATAMLPIEGD
                     TCILLRQYRFAIGEWLCEAPAGTMDPGETPLETARRELIEEAGLSGGNFIPRGFIYTT
                     PGFTTERIHLFEVRDLAPSAEFTKDEDEEIEVVRVPIPEVLAMARDGRIRDAKTLALV
                     FRCLEG"
     gene            complement(6976..7584)
                     /locus_tag="VKO45_06335"
     CDS             complement(6976..7584)
                     /locus_tag="VKO45_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA05530.1"
                     /translation="MKVSEIFRSIQGEGRSQGLITTFVRLSGCNLNCAWCDTPYAREG
                     GKEVPAGDVFQAIEFSSCNRVCITGGEPLLQREEVTALAERLCGAGYQVEIETNGTID
                     FSTLQRFASVCMDVKCPSSGEESDLSLLSRILGNDAVKFVVNDSEDLDYARDVIAANP
                     IPGEYLVSPVYGSDYGALARQVLDRNMPVRFQIQLHRILGVR"
     gene            complement(7571..8200)
                     /locus_tag="VKO45_06340"
     CDS             complement(7571..8200)
                     /locus_tag="VKO45_06340"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HMA05531.1"
                     /translation="METSKGGGPRESAKGRSPSGNQQAEGLVVRQQGEALMGTSKGGG
                     PREKQQGEALMGTSKGRSPRENQRGEAPVVTVIYKEVCFDASHRLLHYEGKCKNLHGH
                     RWRVEVWIAGEIDPETGILVDFNVIRDVIERFDHQVILNRDDPMVACLEKFQPVVKTR
                     GDPSSEVLAGEIRRLLEREFGSGKSRAWVLRIRVWESDTCCAEQEYEGQ"
     gene            8947..9375
                     /locus_tag="VKO45_06345"
     CDS             8947..9375
                     /locus_tag="VKO45_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HMA05532.1"
                     /translation="MGLGKFAARKLARDSKKFRWSDKRYARRLLHLDEKADPLEGSPQ
                     ARGIVLEKIGIEAKQPNSAIRKAVRVQLIKNGRQVTAFCTGDGAINFIDEHDEVEIEG
                     IGGRLGRSMGDIPGVRYVVTKVNNVSLRELVVGRKEKPRR"
     gene            9570..10199
                     /locus_tag="VKO45_06350"
     CDS             9570..10199
                     /locus_tag="VKO45_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HMA05533.1"
                     /translation="MKAKEPAVETVPAAPEKEKEEKEPRLLFNRWDLNEVEVADPSLK
                     RYINLHSMIVPHSSGKFTKQQFAKGEMLIVERLINRLMQTEMNTGQKHRAIRITKDAF
                     EIIHKKTRKNPVQILVDAISQAGPREETVRLKYGGINVPKSVDTAPLRRVNSAIRFIC
                     EGVYTASHKSKKHVPEVLADELIAAARGDSKCYSVTKREERERIAKASR"
     gene            10228..>11618
                     /locus_tag="VKO45_06355"
     CDS             10228..>11618
                     /locus_tag="VKO45_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HMA05534.1"
                     /translation="MTRAEKIVQRVTTLMDKPDHIRNIGIVAHIDHGKTTLSDSLLAG
                     AGMISEELAGKQLFMDSDEMEQARGITIDASNVSMVHEYEGGEYLINMIDTPGHVDFG
                     GDVTRAMRAVDGAVVLVDAVEGPMPQTETVLRQALKEQVRPILFINKVDRLVNELKVD
                     ETEMQIRLGKVIDKINKLIRGMNEEMYQKGWKIDAANGSVAFGSALYKWAVSVPYMKK
                     SGVSFKDVYNKCRDNDQKWLAKQSPICSVILDMVVHHLPNPLEAQTRRINVIWHGDKT
                     TPEGKAMLACDPNGPVCLMVTDISFDPHAGEVATGRLFSGKVQRGMEVFVMGTAKKGN
                     RLQSVGIFMGPDRNEVEGGIPAGNIIAVTGLKDAIVGSTVTNLMDMTPFESLKHYSEP
                     VMTVAVEAKNMKDLPKLVDVLRQVGKEDPTVKVSINEETGEHLISGMGELHLEIITHR
                     IKRDKGVEIVTSEP"
BASE COUNT         2096 a         3741 c         3789 g         1992 t
ORIGIN      
        1 acctggacgg tagtgccctt cccgcaccgg gctgcctcct cgatccggga cgccgcgagg
       61 gccgcaacgg catcgatgta cacgatccct gtcgagaccc cctcactccg tatctgggcc
      121 cacttctcgt tgtccgggat cccgaggatg ttcccctggt ggacgaagac ctcgttcccg
      181 caggcgggcc cgaggagctt cgcgttcgcc tccggctcct cgacgaagac cttcacccgg
      241 tggccgagca gatccccatc ccatgccgta aaggaacagg gaccgggggc agtggggttg
      301 gccatgccgg tctcgcggat ggcccgggcg atgaacctcc cggtcgccgt ggccggttcc
      361 tccttcaggg agacggcccc ggcgatctcc cggtcggtga gatcccgggg gaagaactgg
      421 gggtacgaga gggcccgcat gtcctctgac ccggtgagga tcatggcgag gcgttccacc
      481 ccgaggccca ggttcatgac cgggacctcg atgccgtact cggcaagggc cgagggggag
      541 tagaccccga aggtggccac ctcaacctcc cctcggaccg ggtgggtcgc gtacacttcg
      601 gtctgggaac cgggcatgta gtacttggac cgtttctcgt cgggttggaa ggtgaacttc
      661 gtgaaaccaa aggccgagag gagggcctcg gcgaccgcct tcccctcctc gttcgtcacg
      721 tcctcgccag ccacgacgca ggaggccgag tggtaggtca tgagccgggt cgggccctcc
      781 tcctgctccc gccggaagca gcggtccacg gagaagaggc ggatggggtg ggggcggcgc
      841 tgccagttcg acgagagggt gaggaaccag ccgctcgtca tgtgactccg gagggtccgc
      901 cgggtggact ccggggcgag ctcccggaac tcggggaaga cccggtccat cacctttacg
      961 acggtggcat catccgcggc aaggaccacg gagatggcgt ggacgaggtc gtccccctca
     1021 acctcgccct tcttgtaggc atggagggtc tccctgagcc gggtctccac atcggggccg
     1081 atgggacggc caaggatctc cccgatggca tcgatccgct ccttcccgat cccgacgttc
     1141 ggccggggga gacccccgag gtagaacacc cggtccagga ccgccatggc ctccgggccg
     1201 aactgccggt acacctcctg ctcgtccacg atgagggggt tgcagcactc ctcgaagccg
     1261 agggagaggt agacctcacg gaggcggctc acggtgtcga acaccgggtg gggccgggcg
     1321 tggccgtacc ggagccgggg gtacttcccg tcgccgcccg gcggggtcag caccccgggt
     1381 ccctcgtgcc acgccttttc gaagtcctcc ctcgcccgat ccctccattt ccctgcgtcg
     1441 aatttcatcc cgtccgctcc aggagcacca cccggctcgc cgggctctcg tccctgatct
     1501 cgtagtcgga gccgcccgcg agagccgagg caaaggcctg gatctccccg tgttcgggca
     1561 tgttctcctt cttcaatcgt tttctactgt atcccaggaa catataaccc ttcacttcca
     1621 cgaaccgggc cccggaatcc tgaatcagcc ccgcgtagag gtccgggcgg tgatcgttct
     1681 cccccctgac gacggtgatc cggatggccg atcttctctt tccgaggatc cccaggctct
     1741 ccaccaccct ctcccagctg tcggtcacgg ggcggcagag cctttcgtac gtctcccggt
     1801 ccggtgcaca gagggagaca taggtctgga aggggcgcgt ctcctcgagg acccagggcc
     1861 gggtgccgtt cgagacaagg aaggtggtgt agccccggga gttgaggatg tccacgagcg
     1921 ccgggaggtg ggggtagcag gtcggctccc cggcgagaga gatggcgacg tgtctcggct
     1981 cgagggcttc gcggaacttc tccggggtca cccggtacga aggcttgtag cctgaaaggg
     2041 ccctcttctg gagatcgaga agccggtcag cgatgagctc cggttggatg tctgtgtcgg
     2101 tcttcggctc atgctcgaag gagcgccagc agaagagaca ccgctgggtg caccggaggg
     2161 tgggggtcat ctggatgcag cggtggcttc cgatcccgta gaaccgggcc ttgtagcaca
     2221 tctccccgcc gcagagagcc ctcttgcacc agaggcaggg cttcaccgcc gccgaggagc
     2281 cgggcgatac gaactggtag ccctgcctcc gcagctgccg ctgcggctcc tcacatggta
     2341 tcgagggcat cgatccccag ggtgaggagc gcctccgaga gcgtgttcgc cgtcgctgtg
     2401 acgagggtga gccggctctc ccgggtcacc ccctcgctct tgaggaccgg gacatgctgg
     2461 tagaaggtgt tccacaggtt cgcgagctcg tggacgtagg aggcgaggag gtgagggcga
     2521 agctccaggg tcacctgctc gacgacggcg gggaaccggg cgatggcctt cacgagggcg
     2581 atctccatgg gatcccggta ctcgaaggcg ggggagaagg ccccggcctt ttcgaggatg
     2641 gagcaggccc gggcatgggc gtactggatg tacgggccgc tctgccgctc gaagtccagg
     2701 gcctccttcc agtcgaagac catggccttc tcgggagaga tcttcaccat gtcgtaccgg
     2761 atggcggcga tggccaccga ccgggcgatg gccctcctcg ctgactcggg gagctcggtc
     2821 cggcgcgagg acacctcgtc gagggcccgg gaggccacct cggcgaggag ggcatccgcc
     2881 gagacgaact tcccctgccg ggtggacatg gacccctcgg ggagggagac gaactcgaag
     2941 tggacgatgg tcgggggggt ctcgccgagg agccccaggg tggtcacgag ctgggccccg
     3001 atgagtttat ggtccgcgcc gaggacatcg atgatccggc cgaagttcct cgccttccag
     3061 atgtggtagg cgatgtccct cgccgcgtac accgtggtcc cgtcgctcct ccggaggatg
     3121 tatggcttct cgaagccggc cgccgtgagg tcgagccaga cggtctcgtc ctcccggtac
     3181 tcggcgaggc gctttatcct cccgagcacc cgctccacgt ccccgttcct cacgaaatcg
     3241 ctctcccaga tgaaccggtc atgggagaca ccgaggtctg agagggtctt cttgatcccg
     3301 tcgaggcaga tggtgaccgc cgaccggaac ttccgcatga cctcagggtc cccggcctcc
     3361 accttctgca tgagtccgtc gatttcgggg gcggaacccg ggctgccttc caggtcccgg
     3421 ttcgcctgga tgtaggccct cgcgatccgg tcgtccccct tctcccccgg gtcccctggc
     3481 ccgtaccggt ccaccgccca gacgacaatg gcgatctgcc tccccatgtc gttcacgtag
     3541 tagtgggtct cgatccggtg gcccgccttc cggaaggcac gggcgaggca gtccccgatg
     3601 atggtgttcc ggatatgccc gacgtggagg ggcccgttcg ggttcgccga ggtatgctcg
     3661 atgaccaccc tcccctcccg gccgggaagg gtcccgtagc cgggcttcac cgcctcccgg
     3721 atcgagtcgc ttagatactc cgccccgaag atgaagttca ggtacggccc cttggcttcg
     3781 atccggatcc ccgcgaggtc cggccggagg gagagccgct tcgcgagctc gccggcgatg
     3841 gccgctggag agcgccgctc ggcctttgcg agggaaaagg cgatggtcga ggccaggtcg
     3901 gcatggatcc cgccgtccgt gagggttgga tccgcaccgc cggtctcctc ccggatggct
     3961 ccggccaccc ggcgggagta gtcgaggaac atcctaatac cccgtcctgt accggaggat
     4021 ggcggcgatc cccccgaagg cgttgaagag catcgacccc tcctcgaact cgtcggagat
     4081 gatctccacc ttcgtcccgg tccggtcggc gagctgggtg agggcctcga cgatgtccac
     4141 ctcctcctcg atgacgaggg gggtcgagca ggaggggcag cccccgagct cgatctcgtg
     4201 gatcttcacc ccggcctcgg tctggaccgt ggtctcgtgc cggtgggtgc agttggggca
     4261 cttgatccgg acatggagct tcctgagccg ggaggagagg atgagggtgt ccacggcccc
     4321 cccctcgaga ttcttcctga ccgattcctc gccgtaggcc gcgttcccgt tctccttcac
     4381 gagctcctgg aggaaccggt tcatgatggt cttctcccgg acgatgtcca tccccttgag
     4441 ggcgtcctgg gcggcgtcga cgagttcggt gaggccgctc tcgttcgtat acgagacatc
     4501 gaagaggccg atgatcctct tctggatctc gtggtggagg aaaccgccgg cctcgaactc
     4561 ctccttcgtg ggggagggcc ccccgatgag aacccccttg aaccgctcga agaattcctt
     4621 ctcggcgagg aaggtggcgc tcgccctctc ccccaccttc ttgaagaact cgttgatggc
     4681 gatgagccgg agccgcgcaa accggaccga ggactggccg cccttccgct gcttcccggg
     4741 gaccgtggag gtggccccgg agacggcctc gatccggtcc ccccggagga acccccagta
     4801 ggcctcccgc cggtcgatga cgatgaggcc gtagacctgc ttctcgacga gcatgctcct
     4861 cagggggtcg agctcgaact gggaggagca tcggtacatg tagaggttga ggggttccgg
     4921 gggctcgatg atggtgcact cgaggtcggt ccggtcaccc ccgaggagga cggtcccgca
     4981 gaagaccgcc atgccgttgg gcgggggccg cttgtagtac ttgagccggg cgaggatgga
     5041 ggagatggcc gactggacgt tcgtcctcgt ctgcttggac ttgatgttcg agcactggcc
     5101 gtactcgtcc ctgagctggg cggtcacgtc atggatctgc ttgtcgggcg ggatgtagag
     5161 ggtgatgagc tcggtcccgc tcccctcctt tgcctcgagc ttctcgaggg tcttcttgaa
     5221 ctcgtaccgc cggcgggcct cgtcctgctc ctgccccgcc tcgaccgtct tctccgcgct
     5281 cacggatcca tgctccccgg gtggagtatc tcacccggat tcttccatag tttggtgagc
     5341 gatgctatat aaaccatcga gcggagtggg aggttccgaa aggaaccgac ctcgagcaac
     5401 gatgttgcga gggagggttc gtcagaggga gggtccgtag gaaccgtcct cgagcgacga
     5461 cgactattcc ccggagggaa taggagtgcc gcgaagggta atcgtggcca agtcgcgaag
     5521 aaaccgacct ggagcaaccc gccatcaggc gggtgcgacc gagaggggtc accccgaggt
     5581 gcagatctcc atcaccctcc ggtcgacctc ctcgttctcc ccatgggtcc cgttgaagac
     5641 gatgaaggtc ttcgggttcc cggcctggct gaagagctcc tcccccaggg tgaagttgat
     5701 gatggggtcc tcgcgggagt ggaagatcca gacgggtcgg ggggagatct tcccgatggc
     5761 atggtccggg tcgatggaga gaaggaagcg cgaggcattc ccggtgtact ggttcccggc
     5821 gaggccaaag cccgaggtcg acaccccgat gtaaccggcg aagcccgggt ccgcggccgc
     5881 ggcgatggcg gcgtaccgcc cgccgttgga ggcccccatg gcatagacag ggacctgcca
     5941 gcggcccgcg ataatctccc gtgatgcaga gatgtcgcag gcagtcttga agaactccgg
     6001 ccaggccccc aggttgaagg ccatgaagtc aaggtttggg tcgaagggcc tcccccgggt
     6061 ctcccctccg ttgcccctga ggtcgaggac gagggccgta cacccggcca tagcataggt
     6121 ctcgacccga tccctctccc tctctttcgt cactccggcg cccggtgcga ggacgatggc
     6181 accccttgga ttcaccggag aagcgagaag gccgaagacc tcctcgtcga tgttccggaa
     6241 ggaaacctcc ctgagggtaa gggagccgtt cctctccagg atcgattcgt tcacggtcgc
     6301 gggacccggg caggtgagcc cgaggtaccc gtcgttcgtc accgaccagg tgggaccctg
     6361 agaccccgtc cccaggcagc ccgttcccag gatcaggcct gcgaagatga gcccgaggag
     6421 aagagaactc gaacgcatca gccctccagg cagcggaaca cgagggcgag ggtcttcgca
     6481 tcccggatcc ggccatcgcg ggccatggcg agcacctcgg ggatcgggac ccgcaccacc
     6541 tcgatctcct cgtcctcgtc cttcgtgaat tcggcggaag gggcgaggtc acggacctcg
     6601 aagaggtgga tcctctcggt ggtgaacccg ggggtggtgt agatgaatcc ccgggggatg
     6661 aaattcccgc ccgagaggcc ggcctcctcg atgagctccc tccgggcggt ctcgaggggg
     6721 gtctcgcccg gatccatggt tcctgccggc gcctcacaga gccactcccc gatggcgaac
     6781 cggtactggc ggaggaggat gcaggtgtcc ccttcgatgg ggagcatggc ggtcgcatcc
     6841 cccggatgga tcacgatccg ctccttctcc tttcccgagg ggagccggta gagctccttc
     6901 accacccgga gccgcctccc ccggtagata tcggtcaccg gattgccccc gctctgaacc
     6961 ggatcatggt cattctcacc gcactccgag aatccggtga agctggatct gaaacctcac
     7021 cggcatgttc cggtccagga cctggcgggc cagcgccccg tagtcagacc cgtacaccgg
     7081 tgaaaccagg tactcgcccg gaatcgggtt cgccgcgatg acatcacggg catagtccag
     7141 gtcctcggag tcgttcacca caaacttcac cgcgtcattc ccgaggatcc gggagaggag
     7201 ggaaaggtcg ctctcctctc ccgaggaagg gcacttcaca tccatgcaga cggaggcgaa
     7261 cctctggagg gtggagaaat cgatggtccc gttcgtttcg atctccacct ggtagcccgc
     7321 cccgcagagc ctctccgcga gggccgtcac ctcctcccgc tggagaagcg gctccccgcc
     7381 ggtgatgcag acccggttac aggaggagaa ctcgatggcc tggaagacat ccccggcggg
     7441 aacttccttt ccgccctccc gcgcgtaggg ggtatcgcac caggcgcagt tcaggttgca
     7501 ccccgagagc cggacaaagg tggtgatgag cccctggctc cttccctcgc cctgaatgga
     7561 ccggaagatc tcactgacct tcatattcct gttctgcgca gcaggtgtcg gattcccaga
     7621 ccctgatccg gaggacccag gctcggctct ttcctgaccc gaactcccgt tccaggagcc
     7681 tccggatttc cccggcgagg acctcgctgg acgggtcccc ccgcgtcttc accacgggct
     7741 ggaatttctc gaggcaggcg accatcgggt cgtcacggtt caggatcacc tggtggtcga
     7801 acctctcgat gacatcccgg atcacgttga agtccaccag gatgccggtc tccgggtcga
     7861 tctcccccgc gatccagacc tcgacccgcc accggtgccc gtggagattc ttgcactttc
     7921 cctcgtaatg gaggagccgg tgggaggcat caaaacagac ctccttgtag atgacggtca
     7981 ccaccggggc ctcgccccgc tggttctctc tcgggctcct gcccttcgag gtccccatca
     8041 gggcctcgcc ctgctgtttc tctctcgggc ccccaccctt cgaggtcccc atcagggcct
     8101 cgccctgctg tctcaccacc aggccttcgg cctgttggtt cccgctcggg ctcctgccct
     8161 tcgcgctctc tctcgggccc ccgcccttcg aggtctccat ggaggaagct tatgggcgtt
     8221 gggaaatata aggccccttg taaaccggcc cggagctgcc tctcgggact cactctcaat
     8281 tggctcgagg gatagatccc gaaccattgc tctatatctg atcctccttt taatggggtg
     8341 ctctcgggga gggagggttc attagaacct gacctggagc aacgaagtcg cgacactacg
     8401 ccaccccgcc gcacgggcac cccgtgggtt acgggagtgt tgtgatgggc ggttcaacgg
     8461 gatgaggagt ccatcgcaat tcgggggacg ccggagcggc gggggcatac gaggggatgc
     8521 gtatccccga gtatggagca cccatcaggg tgcgatgagc agaagcgagc atcgcaactt
     8581 cattgctcca ggtcggtttc tgatgaaacc tccctccccc aggagcgtcc gccggatcaa
     8641 atgtacggtt ctagtcaagt gttaggttga ttcactgttt ggcttcatag aaacaggact
     8701 gtaacccacc cgccaccccc tccacccacc cgacactgtt caggataccc cggtacccct
     8761 cgcgatccgg aggaggcacc agaacggcgg gggcgatgtc ccgggaccgt tcacgaaaga
     8821 tcagccggtc gtcccttccc gtcggcccgt ccctgccaca atatataagt tctctccgcg
     8881 caaaaattta gacctcaatt gctcaggata cgaatcctat aagaaacgat tgtacgggga
     8941 catcagatgg gactcggtaa attcgcggcg cggaaacttg cgcgtgactc gaaaaagttc
     9001 cggtggagcg acaagcgcta tgcgcgccgg ctcctccacc tcgacgagaa ggcagacccc
     9061 ctggagggtt cgccccaggc acgggggatc gtgctcgaga agatcgggat cgaggccaag
     9121 cagcccaact ccgccatccg gaaggcggtg cgggtgcagc tcatcaagaa cggccggcag
     9181 gtcacggcct tttgcacggg cgacggggcc atcaacttca tcgacgagca cgacgaggtc
     9241 gagatcgagg gcatcggcgg ccggctcggc cggtccatgg gggacatccc gggcgtccgg
     9301 tacgtcgtga ccaaggtgaa caacgtctcc ctccgggagc tcgtcgtcgg caggaaggag
     9361 aaaccgcgca ggtgagcccc atggcggacg accagcaggt accggttgag gagccggtga
     9421 aggcagccct gaaggagccg gcaaaggaac cggcgaagac cccgaagccg aagagggagc
     9481 gggccccgaa gaaggaggag ggggtgaagc ccgcgaagga gccgaaggaa gggaagaagg
     9541 agaaggagga ggcagcccgg gccatcctca tgaaggcaaa ggagcccgcc gtggagaccg
     9601 tccccgccgc cccggagaag gagaaggagg agaaggagcc ccggctcctc ttcaaccgct
     9661 gggacttaaa cgaggtggag gtggcggacc cctccctcaa gcggtacatc aacctccatt
     9721 ccatgatcgt cccccactcg agcggcaagt tcacgaagca gcagttcgcg aagggcgaga
     9781 tgctcatcgt cgagcggctc atcaaccggc tcatgcagac cgagatgaac acggggcaga
     9841 agcaccgcgc catccggatc acaaaggacg ccttcgagat catccacaag aagaccagga
     9901 agaacccggt ccagatcctc gtggacgcca tttcccaggc cggcccccgc gaggagacgg
     9961 tcaggctcaa gtacggcggc atcaatgtcc ccaagtccgt ggacaccgca cccctcagaa
    10021 gggtgaactc ggccatcagg ttcatctgcg agggggtgta tacggcctcc cacaagagca
    10081 agaagcatgt ccccgaggtc ctcgccgacg agctcatcgc ggccgcccgg ggcgactcca
    10141 agtgctactc cgtgaccaaa cgggaggagc gcgagcggat cgcgaaggcc tcccggtaag
    10201 gaaccatcct ttttttcagg tgacaccatg acccgagcag aaaagatcgt gcaaagggta
    10261 accaccctga tggacaagcc cgaccacatc cggaacatcg ggatcgttgc ccacattgac
    10321 cacgggaaga ccaccctctc ggacagcctc ctcgcggggg cggggatgat cagcgaggag
    10381 ctcgcaggaa agcagctctt catggattcg gacgagatgg agcaggcccg gggcatcacc
    10441 atcgatgcct cgaacgtctc catggtccac gagtacgagg gcggggagta cctgatcaac
    10501 atgatcgata cccccggcca cgtggacttc gggggagacg tgacccgggc gatgcgggcg
    10561 gtggacggcg cggtcgtcct cgtggacgcg gtcgaggggc ccatgcccca gacagagacc
    10621 gtgctccgcc aggccctgaa ggagcaggtg cgtccgatcc tcttcatcaa caaggtcgac
    10681 cggctggtga acgagctcaa ggtcgacgag accgagatgc agatccggct cgggaaggtt
    10741 atcgacaaga tcaacaagct gatccgcggg atgaacgagg agatgtacca gaagggctgg
    10801 aagatcgatg ccgcgaacgg ctcggtggcc ttcggctcgg ccctgtacaa gtgggcggtc
    10861 tcggtcccgt acatgaagaa gtccggcgtc tccttcaagg acgtgtacaa caagtgccgg
    10921 gacaatgacc agaagtggct cgcgaagcag agccccatct gctccgtgat cctggacatg
    10981 gtcgtgcacc acctcccgaa ccccctggag gcgcagacgc ggaggatcaa cgtgatctgg
    11041 cacggggaca agaccacccc cgaaggaaag gccatgctcg cctgtgaccc gaacggcccg
    11101 gtctgcctca tggtcaccga tatctctttc gacccccacg cgggtgaggt ggccaccggg
    11161 aggctcttct cggggaaggt ccagcggggg atggaggtct ttgtcatggg aacggcgaag
    11221 aagggcaacc gcctccagtc ggtaggaatc ttcatgggcc ccgaccggaa cgaggtggag
    11281 ggcgggatcc cggccgggaa tatcatcgcc gtgaccggcc tcaaggacgc catcgtcggg
    11341 tccacggtga cgaacctcat ggacatgacc cccttcgagt ccctcaagca ctacagcgag
    11401 ccggtcatga cggtagcggt ggaggcgaag aacatgaagg acctcccgaa gctcgtcgac
    11461 gtgctccgtc aggtgggcaa ggaggacccg acggtgaagg tctccatcaa cgaggagacg
    11521 ggggagcacc tgatcagcgg catgggggag ctccacctgg agatcatcac ccaccggatc
    11581 aagcgggaca agggggtcga gatcgtcacc tccgagcc
//
LOCUS       DATGDI010000165         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_133905, whole genome shotgun sequence.
ACCESSION   DATGDI010000165 DATGDI010000000
VERSION     DATGDI010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133905"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..193)
                     /locus_tag="VKO45_06360"
     tRNA            complement(122..193)
                     /locus_tag="VKO45_06360"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(159..161),aa:Ala,seq:cgc)
     gene            complement(275..1285)
                     /locus_tag="VKO45_06365"
     CDS             complement(275..1285)
                     /locus_tag="VKO45_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HMA05535.1"
                     /translation="MQEIEKVKILQTEREALKKRSKEVARERERTGQDYAEVVCPECG
                     GRQLVHDYERAELVCQNCGLVIDEDFIDRGPEWRAFDNDQRIKRSRVGAPMTFTIHDK
                     GLSTMIDWRNRDSYGRAISSKNRAQLYRLRKWQRRIRVSNATERNLAFALSELDRMAS
                     ALGLPRNVRETAAVVYRDAVDKNLIRGRSIEGVAAAALYAACRQCSVPRTLDEIAEVS
                     RVSRKEIGRTYRFISRELGLKLLPTSPIDYVPRFCSGLNLKGEVQSRAVEILRQAGER
                     ELTSGRGPTGVAAAAIYISSILGGERRTQREVAEVAGVTEVTIRNRYKELAEKLDIEI
                     IL"
     gene            complement(1600..2256)
                     /locus_tag="VKO45_06370"
     CDS             complement(1600..2256)
                     /locus_tag="VKO45_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HMA05536.1"
                     /translation="MYDLHTHTTLSDGELLPIELVRRASVLGYRTLAIADHVDPGNLS
                     PVIAAQIPLKELAAAYGVTLLIGVEITHVPPPHISLLARRAKKEGAEIVVVHGETVVE
                     PVAPGTNMAACTCDEVDLLAHPGLISPEAAREAARREIALEITSRAGHNRTNGHVVLL
                     ARSAGCTIVVDSDAHGPGDLLDPDAREAVARGAGLSIPEASAALGLNIEKWLSRRRTI
                     "
     gene            complement(2261..>2552)
                     /locus_tag="VKO45_06375"
     CDS             complement(2261..>2552)
                     /locus_tag="VKO45_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle subunit SRP19/SEC65
                     family protein"
                     /protein_id="HMA05537.1"
                     /translation="IFSQMKPSGERILYPCYFDATLDRSSGRRVPASLAVKGPTLPDL
                     ERAVKRERLRFRAEEKHHPTHWSKRDGRLVVAWEGSKEELLRRVARSLDVKR"
BASE COUNT          461 a          800 c          809 g          482 t
ORIGIN      
        1 aacccgcggc cttgggcgtt ccgaaccgag ggctccgccc tcgacccggg gcgaccttcg
       61 gtcgccaaaa ggacgatcta atatccggat gatctgaacg gttcaggaaa aaaaatcgga
      121 gtggacccgg cgggatttga acccgcggcc tttacgttgc gaacgtaacg atctacccct
      181 gatctacgag cccgcgaggt acagattctg ccccaagaag atatagtcct tactaaggga
      241 cgaagataca aaggactgag aaaaagaggg gaattcagag gatgatctca atgtcgagct
      301 tctcggcgag ctccttgtac cggttccgga tggtcacctc ggtgaccccg gccacctcgg
      361 cgacctcgcg ctgtgtccgc ctctctcccc cgaggatgga ggagatgtag atggcggccg
      421 ccgcgacgcc ggtcgggccc ctgccagagg tgagctccct ctcgccggcc tggcggagga
      481 tctccaccgc ccggctctgg acctctccct tcaggttgag gcccgagcag aaccggggca
      541 cgtagtcgat gggcgaggtc gggaggagct tgaggccgag ctccctcgag atgaaccggt
      601 aggttctccc gatctccttc ctcgagaccc gggagacctc ggcgatctcg tcaagggtcc
      661 tcggaacgga gcactgccgg caggccgcgt agagggccgc agcagctaca ccctcgatgg
      721 accggccgcg gatcaggttc ttgtccacgg cgtcacggta caccacggcc gccgtctccc
      781 ggacattcct cgggagcccg agggccgagg ccatccggtc caattccgag agggcaaagg
      841 ccaggttccg ctccgtggcg ttggagaccc ggatccggcg ctgccacttc ctgagccggt
      901 agagctgggc ccggttcttg gaggagatgg ccctcccgta cgagtcccgg ttccgccagt
      961 cgatcatggt ggagaggccc ttgtcatgga tggtgaaggt catgggggcc cccacccggg
     1021 acctcttgat ccgctggtcg ttgtcaaaag cccgccactc ggggccgcgg tcgatgaagt
     1081 cctcatcgat gacgaggccg cagttctggc agacgagctc ggcccgctcg tagtcatgga
     1141 cgagctgcct gccgccgcac tccgggcaga caacctctgc atagtcctgc cccgtccgct
     1201 cgcgctcccg ggcaacctcc ttggagcgct tcttcagggc ctctctctct gtctggagga
     1261 tctttacctt ctcaatctcc tgcatgggtc ctcacctacc tcccaaatag tttctctccg
     1321 acttccaatc cgcaggtgcc gtaacagagc acggccgcat acggtttctc gatgttccca
     1381 aagacgtcga cgaccttgcc aaccgcccga aggcgtctat ctaccacgtc gctatataag
     1441 cgcgggagtt tcgaggcgtc acaatgaatg agtaacaggc gtttccctga acggtgaaga
     1501 acgctgccta aactcctcaa atttacctcg aactttcacc tgtgtttagg ttacttatat
     1561 atatggcgct tttttatata taaggattgc ggtcaacgat taaatagttc tcctacggga
     1621 gagccatttc tcgatattta aacctagtgc ggccgaggct tccgggatcg agagaccggc
     1681 cccgcgcgcc acggcttccc gcgcgtccgg gtccaggaga tcgccggggc cgtgggcatc
     1741 cgaatccacg acaatggtgc aacccgcaga ccgggccagg agcacgacat gaccgttcgt
     1801 ccggttgtgg ccggcccggg aggttatctc gagggcgatc tccctccggg ccgcctcccg
     1861 ggcggcctcg ggagagatga ggccgggatg ggcgaggaga tccacctcat cgcaggtgca
     1921 ggccgccatg ttcgtgcccg gggcgaccgg ctcgacgacg gtctcgccat gcacgacaac
     1981 gatctctgcc ccttccttct tcgcccgccg ggcgagaagg gagatgtggg ggggcgggac
     2041 atgggtgatc tcgactccaa tgaggagggt caccccgtag gcggcggcga gctccttcag
     2101 gggaatctgc gccgcgatga ccggggagag gttcccgggg tcgacgtggt cggcgatggc
     2161 gagggtccgg tacccgagga ccgaggccct gcggacgagc tcgatgggga gcagttctcc
     2221 atcggaaagg gtggtatgag tgtggaggtc gtacacgggt tcacctcttc acgtcgaggg
     2281 atcgggccac ccgccggagg agctcctctt tggagccctc ccaggcgacg acgagcctgc
     2341 catccctctt ggaccagtgg gtgggatggt gtttctcctc ggcccggaac cggaggcgtt
     2401 ccctcttcac ggcccgctcc aggtccggga gggtgggccc cttgaccgcg agggaggcgg
     2461 gaacccgcct ccccgaggac ctgtccaggg tggcatcgaa gtagcagggg tacaggatcc
     2521 tttctcccga aggtttcatc tgagagaata tt
//
LOCUS       DATGDI010000166         3699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_395862, whole genome shotgun sequence.
ACCESSION   DATGDI010000166 DATGDI010000000
VERSION     DATGDI010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395862"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1395)
                     /locus_tag="VKO45_06380"
     CDS             complement(214..1395)
                     /locus_tag="VKO45_06380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMA05538.1"
                     /translation="MSSSRTIAGLSIAHLVTDLYGPALPAIISLLILRQGYSYLAAGL
                     LITVYNVVSSLAQPGIGWVHDRKGRQVPPALSILICGAFISLLGLAPGFSAMLTFCAI
                     AALGHAAFHPVALALTGREATGENRGRILSYFVVGGNLGFALGPLAAGVAMEMLGQEG
                     ILFMILPALAMAVALPLLLPSSSHHPQGNGPREGKASVPVLWTPIAVLVTAASLRSMV
                     IFGSIAYLPTFLAEQGFDLLTANSLLTLVLLVGVGGQVLGGAASDRFGRKETILAGMA
                     GTVLFLAGFLLFRGPAGLLSLMLFGFFLWSSFSVTLAISHEFLPDNLGLASGLLLGLS
                     MGVGGLGVAVIGAAGDSLGLGAALWVLLGVTLLATHLFILLPYPWKEREFVGTGHTDD
                     S"
     gene            complement(1399..2010)
                     /locus_tag="VKO45_06385"
     CDS             complement(1399..2010)
                     /locus_tag="VKO45_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="METTL5 family protein"
                     /protein_id="HMA05539.1"
                     /translation="MKLRTLEIALEGLEGFEKPDPGREQYSTPAVFAARLLHHAHLLG
                     DIEGKAVCDLGSGTGVLALGAALLGADRVVGVELDPAALEAARRNAARLGAEVTFIQG
                     DVRDPGLISGLGRFDTVIMNPPFGAQDPHADRPFVDAALVLAPVVYGIFNSGSLPFLE
                     AYTDGRAEIEFAIGGILPLKRTLPFHRKELVEIEVEILRMKRR"
     gene            complement(2012..2953)
                     /gene="dph2"
                     /locus_tag="VKO45_06390"
     CDS             complement(2012..2953)
                     /gene="dph2"
                     /locus_tag="VKO45_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HMA05540.1"
                     /translation="MEDLSDLVRDLRGRGARRVALQFPEGLKRRAPEMALRLKAAGFE
                     VIVSGDPCYGACDLPLPAMTLADVLVHIGHAPVEDRPGVIYDLHPMDFPVEVLAKALP
                     LLSGNRIGLVTTSQHLHLLPSLVRFLKEQGIDAVVAGGTGRARYPGQVLGCSFSGARE
                     TGCAEILYVGTGLFHPEGIQLATGASVIALDPFLGTAARISAERLKRRRFALIEKARE
                     AERIGILVSTKSGQQRLGLARRLAGLSEKAVLVALCEVSPAELMNLGFDCYVNTACPR
                     LAYDDQVRFPVPVLSPPEFEILCGARAWEDFTLDEWD"
     gene            complement(2937..3488)
                     /gene="hpt"
                     /locus_tag="VKO45_06395"
     CDS             complement(2937..3488)
                     /gene="hpt"
                     /locus_tag="VKO45_06395"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine/guanine phosphoribosyltransferase"
                     /protein_id="HMA05541.1"
                     /translation="MLERLIESLETCPIVKRGDYDYFIHPITDGVPRVDPALLRDVCS
                     SMVKVLDLRDVKLFVVAEAMGIHIGTTLSLATDIPFTIVRKRKYELPGEVAVHQTTGY
                     SKGELYLNGVRKGDRVVIVDDVLSTGGTMKALIQALEYVGADIADICVAIRRGKAEVG
                     RPVKSLVDIEVVRGKVHIVGRSL"
     gene            complement(3481..>3699)
                     /locus_tag="VKO45_06400"
     CDS             complement(3481..>3699)
                     /locus_tag="VKO45_06400"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844683.1"
                     /note="catalyzes the decarboxylation of L-tyrosine to
                     produce tyramine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase MfnA"
                     /protein_id="HMA05542.1"
                     /translation="ENTERLIAGLETFGIPRAVTPDVNVATFICDRAPAGWQVSRTRR
                     GHLRIVCMPHVSRDEVEAFIAAMGDLYA"
BASE COUNT          674 a         1190 c         1299 g          536 t
ORIGIN      
        1 ggatatcctc gttttactcc tggtgtgctc tcgggggggc tcacccgccc ccccgccgca
       61 cgggcgcccc cccccccgtt tacggtatcc ctgattaggt agcttcccca aatgaccggc
      121 ctatcctcac gcgggggacg ccgaagcggc gggggtgagc ggagcgaatc ccccaggagc
      181 gtccgacaat tccggaagaa tgcctccgct gtattatgaa tcatccgtat gtccagttcc
      241 cacaaattcc ctctctttcc acgggtacgg gagcaggatg aagagatggg tggcaaggag
      301 ggtcaccccg aggagcaccc agagggccgc cccgagaccg agggaatccc cggccgcccc
      361 gatgaccgcg acgccgagcc ccccgacgcc catggagagc ccgaggagaa ggcccgaggc
      421 gaggccgagg ttatcgggca ggaactcgtg ggagatggcg agggtcaccg agaagctcga
      481 ccagaggaag aaaccgaaga gcatgaggga caggaggccg gccgggccgc ggaagaggag
      541 gaagccggcg aggaagagga ccgtcccggc catgcccgcg aggatggtct ccttccggcc
      601 aaaccggtcc gaggccgccc ccccgaggac ctgcccgccc accccgacga ggagcacgag
      661 ggtgaggagg ctgttggccg tgaggaggtc gaaaccctgc tcggcgagga aggtgggcag
      721 gtaggcgatg gagccgaaga tcaccatgga gcggagggat gcggcggtaa caagaaccgc
      781 gatcggagtc catagtaccg gtacggacgc ctttccttca cgaggaccgt ttccctgcgg
      841 atgatgagag gatgacggaa gcaggagagg gagggcaacg gccatcgcga gagccgggag
      901 gatcatgaag aggataccct cctggccaag catctccatg gcaactccgg cggcgagagg
      961 cccgagggcg aagcccaggt ttccccctac gacgaagtag gagagaatcc ggccccggtt
     1021 ctccccggtt gcctcccggc ccgtcagggc gagggcgacc gggtggaagg ccgcgtggcc
     1081 gagggccgca atggcgcaga aggtgagcat ggcagaaaag cctggggcga ggccgaggag
     1141 ggagatgaaa gccccgcaga tgaggatgga gagggccggg gggacctgcc tccccttccg
     1201 gtcatggacc cagccgatgc cgggctgggc gagggaggag accacgttgt acacggtgat
     1261 gaggagcccg gccgcgagat acgagtatcc ctggcggagg atgaggaggg agatgatggc
     1321 cgggagggcc ggcccataga gatcggtgac cagatgggcg atggacaggc cggcgatggt
     1381 gcgggaggag gacacggcct accgcctctt catcctcagg atctcgacct cgatctcgac
     1441 cagctccttc cggtggaagg ggagggtcct tttcaggggg aggattcctc cgatggcgaa
     1501 ctcgatctcc gcccttccgt ccgtgtacgc ttcgaggaag ggaagggaac ccgagttgaa
     1561 gatcccgtac acgaccggcg cgaggacgag ggcggcatcc acaaaggggc gatcggcatg
     1621 gggatcctgc gctccgaagg gggggttcat gatcaccgtg tcaaaccggc ccagcccgga
     1681 gatcagcccc ggatccctca catctccctg gatgaaggtc acctccgccc ccagccgtgc
     1741 cgcgttcctc ctcgcggcct cgagggcggc ggggtcgagc tccaccccga caacccgatc
     1801 cgcaccgagg agtgcagcac cgagggcgag gacccccgtc ccgctcccga ggtcgcagac
     1861 cgcttttccc tcgatatcgc ccaggaggtg ggcgtggtgc aggagccggg ctgcgaacac
     1921 cgccggcgtg ctgtactgct ccctccccgg gtcgggcttt tcgaacccct ccagcccttc
     1981 gagggcgatc tcgagggtcc ggagcttcat gtcagtccca ctcgtccagg gtaaagtcct
     2041 cccaggcccg ggcgccgcag aggatctcga actcgggcgg ggagaggacg gggaccggga
     2101 accggacctg gtcgtcgtac gcgaggcggg ggcaggcggt gttcacatag cagtcgaagc
     2161 cgaggttcat gagctcggcg gggctcacct cgcacagggc cacgaggacc gccttttccg
     2221 agaggccggc cagacgccgg gcgaggccga ggcgctgctg cccgctcttc gtggaaacga
     2281 ggatcccgat gcgctccgcc tccctcgcct tctcgatgag ggcgaaccgg cgccgcttca
     2341 gcctctcggc cgagatccgg gccgcggtgc ccaggaacgg gtcgagggcg atcaccgaag
     2401 cgccggtcgc gagctggatc ccctcggggt ggaagaggcc ggttcctaca tagaggatct
     2461 cggcgcagcc ggtctcccgg gcaccggaaa aggagcagcc gagcacctgc ccggggtacc
     2521 gcgccctccc cgtccccccc gcgaccaccg cgtcgatccc ctgttccttc aggaacctga
     2581 ccagtgaggg gaggaggtgg aggtgctgcg aggtggtgac aaggccgatc cggttcccgg
     2641 agagaagggg gagggccttc gcgagaacct ccacggggaa gtccatgggg tggaggtcat
     2701 agatgacccc gggccggtcc tcgacggggg catgcccgat atggacgagg acgtccgcga
     2761 gggtcatggc cgggagggga aggtcgcagg cgccgtagca ggggtccccg ctcacgatga
     2821 cctcgaaacc cgccgccttc agccggaggg ccatttccgg ggccctccgc ttcagcccct
     2881 ccgggaactg gagggccacc cgccgggccc cgcgcccgcg gaggtcccgg accaggtcag
     2941 agagatcttc caacgatatg cacctttccc cggacgacct cgatgtccac cagggatttc
     3001 accggccttc cgacctccgc cttcccccgg cggatggcga cgcagatgtc ggcgatgtcg
     3061 gctcccacgt actcgagcgc ctggatgagg gccttcatgg tgccgccggt ggagaggaca
     3121 tcgtccacga tgacgacccg gtcccccttc cgcaccccgt tcaggtagag ctcccccttc
     3181 gagtaaccgg tggtctggtg aacggcgacc tcccccggaa gctcgtactt ccgcttccgg
     3241 acgatggtga aggggatgtc ggtcgcgagg gagagggtcg tcccgatgtg gatccccatg
     3301 gcctctgcga cgacgaagag tttcacgtcc cggaggtcga ggaccttcac catggaggaa
     3361 cagacgtccc ggagaagggc cggatccacc ctcggtaccc cgtcggtgat ggggtggatg
     3421 aagtagtcat agtccccccg cttcacgatg gggcacgtct ccagggattc gatgagtcgt
     3481 tcaagcatac aggtcaccca tggcggcgat gaaggcctcc acctcgtccc ggctcacgtg
     3541 gggcatgcag acgatccgga ggtggcccct ccgggtccgg gagacctgcc accctgccgg
     3601 ggcccgatcg cagatgaagg tcgccacgtt cacgtccggt gtcaccgccc tcgggatccc
     3661 gaaggtttcg aggccggcga tgagtctctc cgtgttctc
//
LOCUS       DATGDI010000167         1551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_134130, whole genome shotgun sequence.
ACCESSION   DATGDI010000167 DATGDI010000000
VERSION     DATGDI010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134130"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1551
                     /gene="glgP"
                     /locus_tag="VKO45_06405"
     CDS             <1..>1551
                     /gene="glgP"
                     /locus_tag="VKO45_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HMA05543.1"
                     /translation="SLLGNYLRSMGLSLDAFMALGSPNEHGPRNSFNMAIMALKSSSK
                     TNAVSQLHRTVSRRIWQEIWPSLPEVDIPIDSVTNGIHIPSFISDDMGRLLDRYLGRR
                     WAEDPDNASVWMRVNKIPDAELWRTHERRRERLITFTRQRLQEQLQRRGGQMEEIQAS
                     GQVLHPEALTIGFARRFATYKRGDLILKDPVRLAKILNHPQHPVQIIFAGKAHPQDAA
                     GKEVIKHIVHLARQPEFRDRIVFIEDYELTVARYMVQGSDIWLNNPRRPLEACGTSGM
                     KAAANGAINMSVLDGWWAEGYQQDVGWALGSGEEYQDAAYQDFVESQSIYDLLERNAV
                     PLFYDRGKDNLPRGWISLMKNSMLNLVGQFNSHRMLQDYVHQLYIPSALNWRKMEADG
                     YRGAREFTAWIQRIRQHWSQLRILEKKAETRGLIQFGQTLKVEVVLHLGSLSPNDLTV
                     DIYYGRVDSKAEFLDRETISLRDVIPRDKTTVFRGEIPCKKVGRFGFRVRVLPSHPLL
                     SNPNSLGL"
BASE COUNT          320 a          487 c          453 g          291 t
ORIGIN      
        1 gctccctgct ggggaattac ctgcgctcca tggggctctc cctggatgct ttcatggccc
       61 tgggttctcc caatgaacac ggcccccgga attccttcaa catggccatc atggccctga
      121 agagctcctc caagaccaac gccgtgagcc agcttcaccg gaccgtctcg cgccgcatat
      181 ggcaggagat ctggccttct cttcccgaag tcgacattcc catcgacagc gtgaccaatg
      241 ggattcacat cccctccttc atatccgacg acatgggccg gctcctggac cgttacctcg
      301 gaaggcggtg ggccgaagat ccggacaacg cctccgtgtg gatgcgggtc aacaagattc
      361 ccgatgccga gctctggcgg acgcacgaac gaaggcggga gaggctcatc acctttaccc
      421 ggcagaggct gcaggaacag cttcagcggc gcggcgggca gatggaagag atccaggcct
      481 ccggccaggt tctccatccc gaagccctga ccatcgggtt cgcccgcaga ttcgccacct
      541 ataagagggg agacttgatc ctgaaagacc cggtccgtct ggccaagatt ctgaatcatc
      601 cccagcaccc ggtgcagatc atctttgccg gcaaggccca tcctcaggat gccgccggga
      661 aagaggtgat caagcacatc gtccatctgg cgcggcaacc ggaattccgg gaccggatcg
      721 ttttcatcga agactacgag ctgaccgtgg cccggtacat ggtccagggt tcggacatct
      781 ggctgaacaa cccccgccga cccctggagg cctgcggcac gagcgggatg aaggcggcgg
      841 ccaacggggc gatcaacatg agcgtcctgg acggctggtg ggcggaggga taccagcagg
      901 acgtgggctg ggccctcggg tccggggaag aataccagga tgcggcctac caggactttg
      961 tggaaagcca gtccatttac gatctcctcg aaaggaatgc cgttcccctc ttctatgacc
     1021 ggggaaagga caacctgccc cggggatgga tcagcctgat gaagaattcc atgttgaacc
     1081 tggtgggcca gttcaactcc caccgcatgc tccaggatta cgttcatcaa ttgtacatcc
     1141 cctcggctct gaactggaga aagatggaag cggacgggta tcggggggcc cgcgagttta
     1201 ccgcctggat ccagcgcatc cggcaacact ggtcgcagct tcggatcctg gaaaagaaag
     1261 ccgaaacccg ggggcttatc cagtttggcc agaccctgaa agtggaagtc gttctgcatc
     1321 tggggtccct atcccccaac gacctgaccg tggatattta ttacggccgt gtcgattcca
     1381 aggccgaatt cctggaccgg gagaccattt ccctccggga tgtcatcccg cgggacaaga
     1441 cgaccgtctt ccggggggaa atcccctgca agaaagtggg ccgcttcggg ttccgcgtcc
     1501 gggttctccc ttcccatccc ctcctttcca acccaaactc tctggggctg a
//
LOCUS       DATGDI010000168         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_105248, whole genome shotgun sequence.
ACCESSION   DATGDI010000168 DATGDI010000000
VERSION     DATGDI010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105248"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1790
                     /locus_tag="VKO45_06410"
     CDS             132..1790
                     /locus_tag="VKO45_06410"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HMA05544.1"
                     /translation="MRLLLLQLNPVIGDLAGNAEKIRREVIAAGRDSADLVVTSELVL
                     PGYPPRDLLLFRGYIGRARETAEALARDLEGYPPVLLGTVQPNPSPVGRPLSNVALLL
                     HGGKILRTFGKTLLPTYDVFDEDRYFEPARGPQILDLGGTKLGISICEDIWNDADFWN
                     QKRYHSDPIRELVEAGARCLVNISASPFTAGKQQLREKMLSGIARKYRIPLLYVNQVG
                     GNDDLVFDGRSCAFSAKGELIARAAPFEEDRLSLDILEGGPAGIHADDFSPESEIWNA
                     LVLGTRDYVRKTGFSKVLVGLSGGIDSSLVAAIAVEALGRENVLGVLMPSPFSSGGSV
                     TDAEALGEALGIRTTVLPIEGIMKGFDGTLSGEFSGLPRDITEENLQARIRGTLLMAL
                     SNKFGSLLLSTGNKSEVAVGYCTLYGDMNGALAVIADVPKTMVYRVARWLNARGSNPV
                     IPEPVFTKAPSAELRPDQTDQDTLPPYDLLDEILHYHIEQHESAAEIMARGYDPGVVY
                     RVTHLVKGAEFKRKQAPPGIKVTDRAFGTGWRMPLAAREWDLPR"
     gene            1834..>2238
                     /locus_tag="VKO45_06415"
     CDS             1834..>2238
                     /locus_tag="VKO45_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMA05545.1"
                     /translation="MSQRRVVVTGAGGFIGHHLVTALKKQGNWVRGVDIKYPEFSRSD
                     ADQFEILDLRRWDSCLQATRGADEVYALAADMGGMGFISCHHAEILHNNSLINTHTLE
                     AARINGVKRYFYTSSACVYPEYKQTEAKVVPLK"
BASE COUNT          431 a          735 c          683 g          389 t
ORIGIN      
        1 ctcctctggg cctcaaagcc ggttgtggag aatcacgacc agggaccacc caaaggagtt
       61 cgtgtatcgg ctatctcggg taaaatcccc gagggaatcc tttttgagcc atccagaata
      121 atataacggc aatgaggctc cttctcctcc agctgaaccc ggtcatcggg gatctcgcgg
      181 gcaacgccga gaaaatccgg cgggaggtca tcgccgccgg ccgcgattcc gcggacctcg
      241 tggtcacctc agagctcgta cttccggggt atcccccgag agacctgctc ctcttccggg
      301 gctacatcgg gagggcccgg gagaccgcag aggccctggc cagggacctg gaggggtacc
      361 cgccggttct cctggggacc gtccagccca atccatcccc cgtcggccgc cccctgtcca
      421 acgtcgccct cctgctccac gggggcaaga tcctccggac cttcggaaag actctccttc
      481 ccacctacga tgtcttcgat gaggaccgct acttcgagcc ggcccggggg ccccagatcc
      541 tcgatctcgg ggggacgaag ctcggcatct ccatctgcga ggacatctgg aacgacgccg
      601 atttctggaa ccagaaacgc tatcactcgg atcccatccg ggagctcgtg gaggcggggg
      661 cccggtgcct ggtgaatatc tcggcatcac ccttcaccgc ggggaagcag caactacggg
      721 agaagatgct ctccgggata gcccgcaagt accgtatccc cctcctctac gtgaaccagg
      781 tgggggggaa cgacgacctg gtcttcgacg ggcgaagctg tgccttcagc gcaaagggcg
      841 agctcatcgc ccgggcggcc cctttcgagg aggaccggct ctccctggac atcctggagg
      901 gggggccggc cgggatccat gccgatgact tctcgccgga gtccgagatc tggaacgccc
      961 tcgtcctcgg gacccgggac tatgtccgga agacagggtt ttcgaaggtc ctcgtcgggc
     1021 tctcgggcgg gatcgactcc tccctcgttg ccgccatcgc ggtggaagcc ctggggaggg
     1081 aaaacgtcct cggggtcctc atgccctcgc ccttctcgag cggggggagc gtgaccgatg
     1141 cagaggccct gggagaggcc ctgggcatca ggaccaccgt cctccctatc gaggggatca
     1201 tgaagggctt cgatggcacc ctctccgggg aattctctgg cctcccccgg gacatcaccg
     1261 aggagaacct gcaggcccgg atccggggca ccctcctcat ggccctctcc aacaagttcg
     1321 ggtctcttct cctctcaacc gggaacaagt cggaggtcgc ggtggggtac tgcaccctct
     1381 acggggacat gaacggggcg ctcgccgtca tcgccgacgt gccgaagacc atggtgtacc
     1441 gggtcgcccg gtggctcaat gcgcgggggt cgaaccccgt catcccggaa cccgtcttca
     1501 caaaggctcc ctctgcggag ctccgaccgg accagaccga ccaggacacc ctccccccct
     1561 acgacctcct ggacgagatc ctccactacc acatcgagca gcacgaatca gcggcggaga
     1621 tcatggcccg gggatatgat cccggtgtcg tataccgggt tactcacctg gtgaagggtg
     1681 cggagttcaa gaggaagcag gctccgccgg ggatcaaggt caccgaccgt gccttcggca
     1741 cgggatggag gatgcccctc gccgcccggg aatgggatct cccccgatga tgaaatcaat
     1801 acgcataagt cgaatcccgg agcatgcatt accatgagcc agcgacgggt agtggtcacg
     1861 ggcgccgggg gtttcatcgg ccaccacctc gtcaccgccc tgaagaagca ggggaactgg
     1921 gtccgcgggg tggacatcaa gtatccggaa ttctcccgga gcgatgccga ccagtttgag
     1981 atcctggatc tccggaggtg ggacagctgc ctgcaggcga cccggggtgc ggacgaggtc
     2041 tacgccctcg ccgcggacat gggcgggatg ggcttcatct cctgccacca tgccgagatc
     2101 ctgcacaaca actccctgat caatacccac acattggaag cagctcgaat caatggcgtg
     2161 aaacgctatt tctatacatc ctctgcttgc gtctaccctg aatacaagca aacagaagcc
     2221 aaagtggtac ctttaaaa
//
LOCUS       DATGDI010000169         4525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_337831, whole genome shotgun sequence.
ACCESSION   DATGDI010000169 DATGDI010000000
VERSION     DATGDI010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337831"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VKO45_06420"
     CDS             complement(<1..183)
                     /locus_tag="VKO45_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HMA05546.1"
                     /translation="MTGRNLITEAQNTWCPGCGNFSIQFALKTLLQDLEKQGTSLDDV
                     VLVTGIGCHAKMADYLN"
     gene            complement(180..1844)
                     /locus_tag="VKO45_06425"
     CDS             complement(180..1844)
                     /locus_tag="VKO45_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HMA05547.1"
                     /translation="MAEHSILIGGKAGEGLNKAGIIIARIMNGLGYRVFMYYDYPSLI
                     RGGHNFSIIRAAPHRIYSHRTGIGFILAMNRETLDLHAHRRSAGTVAVFNSDTVKAEG
                     TGIPLSQIAKEEGAPDIARNTGIVAGFCGVAGIPREVLEGVIRREIPRGLEQNLAIAR
                     RAYAAVEASPSAAIAPVPRPEKPLPFFTGNEAAGLGLLAGGLQAYVAYPMTPTSNLLH
                     FMAGLPADLGLTVVHPENEIGVMLMALGFAFAGRRVAVGTSGGGFCLMNEGFSLSGMA
                     EIPVVVFLGQRPGPSTGLPTYSCQTELHFAANAGQGEFIRFIVAPGDLEETYYWAQAA
                     LSLSWRYQIPSIVLYDKNLAEHGYNFDADSVPPIPELPPVAWDGKSPYRRYAITDSGV
                     SPLAFPPAKDAVVKVNSYAHDEDGITTEEPGMVTRIQEKLLRKGEGLARDLAGMPAVT
                     VTGAKAAPDTLLTWGSTAGPCREVGDSLGLRVVQPVVLNPFPGDRVRDALEGARRIIA
                     VEENATGQLERLIAGHLIRIDGRIRKYDGRPFALDELEARVREVLP"
     gene            complement(2337..2504)
                     /locus_tag="VKO45_06430"
     CDS             complement(2337..2504)
                     /locus_tag="VKO45_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05548.1"
                     /translation="MPMVPTAATIAEMARGRYVAEMMDRRFSKLLFPPAAAMPARFAK
                     RDHPKGSPGAL"
     gene            complement(3005..>4525)
                     /locus_tag="VKO45_06435"
     CDS             complement(3005..>4525)
                     /locus_tag="VKO45_06435"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HMA05549.1"
                     /translation="MSGICTRCLDGCKGMCDIWLSSFRGREVLYPGPFGEMTAGADKD
                     YPVDYSHINIQGYAQGAKGLPAGTDIGPDSAVFYSVNTETSYGWDKKVKLRLPIFTGA
                     LGSTEIARKNWEHFAIGAAISGITIVCGENVCGVDPDLVLDSKGKVKSSPEMDRRIET
                     YKKFHEGYGEILVQMNVEDTRLGTAEYVSSKHNLDTIELKWGQGAKCIGGEIKVKTLE
                     RALELKKRGYIVLPDPTLPEVQKGFKSGAFKEFERHSRLGFVSGDGLLEEVDRLRDVG
                     FSRVTLKTGAYSAVELAMAMRFGSEAKIDLLTIDGAPGGTGMSPWPMMNEWGIPTFYL
                     QALAMEYANILKKKGKRVPDIAMAGGFSSEDGVFKALAIGSPFVKAVCMGRGLMIPGM
                     VGKNIGVWLKEGNLPNTVSKYGKSLEEIFISYEDIRAKYGARMKEIPTGAIGIYTYAQ
                     RFRTGLQQLMAGSRNFTVPSISRKDVMALTEEASKISGIPYVMEAYRDEAEAILGS"
BASE COUNT          810 a         1413 c         1441 g          861 t
ORIGIN      
        1 gttcaggtaa tccgccatct tcgcgtgaca cccgattccc gtgacgagga ccacgtcgtc
       61 cagggaggtc ccctgcttct ccaggtcctg gaggagggtc tttaaggcga actggatgga
      121 gaagttcccg cacccggggc accaggtgtt ctgggcctcg gtgatgaggt tccggccggt
      181 catgggagca cctcccggac ccgggcctcg agctcgtcca gggcaaaggg cctcccgtcg
      241 tacttcctga tcctcccgtc gatcctgatg agatggccgg caatcagtcg ctcgagctgc
      301 ccggtcgcgt tctcctccac ggcgatgatc cggcgtgccc cctcaagggc atcccggacc
      361 cggtcaccgg ggaacgggtt gaggaccacg ggctggacca cccggaggcc gagggaatcc
      421 cccacctccc ggcagggacc tgcggtggat ccccaggtga gaagggtgtc cggagcggcc
      481 ttcgccccgg tgaccgtgac ggccggcatc ccggcgaggt cgcgggcgag cccctccccc
      541 ttcctgagca gcttctcctg tatccgggtc accatcccgg gttcctcggt ggtgatcccg
      601 tcctcgtcgt gggcatagct gttcaccttc acgaccgcat cctttgccgg cgggaatgcg
      661 aggggcgaga cccccgaatc ggtgatggca tacctgcggt aggggctctt cccgtcccag
      721 gccaccgggg gcagttcggg gattggcggg acggaatcgg catcgaagtt gtagccatgc
      781 tcggccaggt tcttgtcata gagcacgatg gagggaatct ggtacctcca ggagagggag
      841 agggcggcct gggcccagta ataggtctct tccaggtccc cgggcgcgac gatgaaccgg
      901 atgaactcgc cctgcccggc gtttgcggcg aagtggagct cggtctggca ggagtaggta
      961 gggagaccgg tggagggacc cggtctctgg ccgaggaaga cgacgacggg gatctcggcc
     1021 atgcccgaga gggagaagcc ctcgttcatc aggcagaagc ccccgcccga cgtccccacg
     1081 gcgaccctcc gtcccgcgaa ggcgaacccc agggccatga gcatcacccc gatctcgttc
     1141 tcggggtgaa ccacggtgag cccgagatcg gccgggagcc ctgccatgaa gtggaggagg
     1201 ttcgaggtcg gggtcatggg gtaggccacg taggcctgga gccccccggc gaggaggcca
     1261 agccccgcag cctcgttccc ggtgaagaag gggaggggct tttccgggcg ggggacgggg
     1321 gcgatggcgg ccgacgggct ggcctctacc gccgcgtagg cccgcctcgc gatggcgagg
     1381 ttctgctcga gcccccgcgg gatctctctc cggatcacac cctcaagcac ctcccggggg
     1441 atccctgcga caccgcagaa accggcgacg atgcccgtgt tcctcgcgat gtcgggagcg
     1501 ccctcctcct ttgcgatctg ggagaggggg atgcccgtcc cctctgcctt cacggtgtcg
     1561 gagttgaaga ccgccaccgt tcccgctgac ctccggtgtg cgtggagatc gagggtttcc
     1621 cggttcatgg cgaggatgaa gccgatcccg gtccggtggg aatagatccg gtgaggggcc
     1681 gcccggatga tggagaagtt atggccgccc cggatcaggg acgggtaatc gtagtacatg
     1741 aaaacccggt atcctaggcc attcatgatc cgcgcgatga tgatcccggc cttgttcagc
     1801 ccttcccctg cttttccgcc gatgaggatg gaatgctccg ccatggtgct ccccgtatat
     1861 gcatacgcat tcgattgata aggataacgg tccggccggg ctgtggccgg ggggtgaaaa
     1921 accggccttc catccacccc ctccggccgg gccgccgtat tacgtgggcc ggggatcgtg
     1981 gatctggaaa agagggagaa ccattcctta tttaagggaa gaggccggta aacgggttgg
     2041 aatcttcaca tggctgtaca tttatatata aacatttcta ttttggataa atataatttt
     2101 caagaatttt atcagacaga tatgtttttt acatgaattc aaaataggaa tctatatata
     2161 tcaatcagat gtagtagcat gtgccagcgg tggcggtgtt gccaccgaca ccgggatcct
     2221 gagtcctgag ctcatcattc cggtgcccag cagcacgcac cctgggcatg aaggagagta
     2281 tccgggatgc cgggcgaatc tcgcgaacac gggacaaatc ggggatccag aagggttcaa
     2341 agcgcaccag gggagccctt gggatggtcc cgtttcgcga accgtgccgg catcgcggcg
     2401 gcgggaggga aaagcaactt cgagaacctc cgatccatca tctccgcgac ataccttccc
     2461 ctcgccatct ccgcaatcgt cgcagccgtg ggcaccatgg gcatggttca tcctgtcctc
     2521 ctcacgcaga tgctggtcgt cgcctgtggt gctccctgcg cagtagaact cctccggccg
     2581 gagaatcacg tcctcccggc cggtatttcg gggcgaatcc caaccacctc cgcatccgaa
     2641 tccatgaaat atccgctctt tccctggatt gaccggggct cattccaggg agctgccccc
     2701 gaggtcaccg catccgtatc aggaggagcg tcccctcccg acagggctgt gcgggcaaag
     2761 accggggaag taacagacca agggggcagc aggaccactg cgaacggaca cctctgcgcg
     2821 aacctctcac caaatatatt ccctttgatg aagcaccctc cttacatttc agaattgcac
     2881 taattccacc agcctttttc agccggtttc cggattcttc cgcaaggaac gatgatcttt
     2941 ctacgggcag gcaccctccc gggactggca ccggggaaaa agagggagag gagaggaggg
     3001 tgggttacga cccgaggatg gcctcggcct cgtcccggta ggcttccatc acgtagggga
     3061 tcccggagat cttcgaggcc tcctcggtga gagccatgac atccttccgg gagatggagg
     3121 gaaccgtgaa gttcctcgag ccggccatca gctgctggag gcccgtcctg aacctctggg
     3181 cataggtgta gatgccgatg gcacccgtgg ggatctcctt catccgtgcg ccgtacttgg
     3241 cccggatgtc ctcgtaggag atgaagatct cctcgaggga tttcccgtac ttggagacgg
     3301 tgttcgggag gtttccctcc ttcagccaga cgccgatgtt cttcccgacc atgccgggga
     3361 tcatgaggcc ccggcccatg cagaccgcct tcacaaaggg actcccgatg gcaagggcct
     3421 tgaatacccc gtcctccgaa gagaagccgc cggccatggc gatgtcgggg acccgctttc
     3481 ctttcttctt caggatgttt gcgtactcca tggcgagggc ctggaggtag aaggtcggga
     3541 tgccccactc gttcatcatc ggccaggggc tcatcccggt cccgccgggg gccccgtcga
     3601 tggtgaggag gtcgatcttc gcctcggaac cgaagcgcat ggccatggcg agctccaccg
     3661 cggagtaggc gccggtcttc agggtgaccc gggagaagcc cacgtcccgg agccggtcga
     3721 cctcctcgag gagcccgtcg cccgacacga acccgagccg ggagtgccgc tcgaactcct
     3781 tgaaggcacc gctcttgaag cccttctgga cctcggggag ggtggggtcg gggaggacga
     3841 tgtagccccg cttcttgagt tccagggccc gctccagggt cttcaccttg atctcgccgc
     3901 cgatgcactt cgcaccctgg ccccacttga gctcgatggt gtccagattg tgcttcgagg
     3961 agacgtactc ggcggtcccg aggcgggtgt cctcgacgtt catctggacg aggatctccc
     4021 cgtatccctc gtggaacttc ttgtaggtct cgatccgccg gtccatctcg ggggaactct
     4081 tcaccttgcc cttgctgtcg aggacgaggt ccgggtccac cccgcagacg ttctctccgc
     4141 agacgatggt gatacccgat atggccgccc cgatggcgaa gtgctcccag ttcttccggg
     4201 cgatctcggt ggacccgagg gctcccgtga agatgggaag ccggagcttc accttcttgt
     4261 cccagccata ggaggtctcg gtgttcaccg agtagaagac cgccgagtcg ggcccgatgt
     4321 cggtccccgc ggggagcccc ttcgcaccct gggcataccc ctggatgttg atgtgggagt
     4381 agtcgacggg gtagtccttg tcggcccccg cggtcatctc gccgaaggga ccggggtaga
     4441 gcacctcgcg cccgcggaaa ctcgagagcc agatatcgca catccccttg catccgtcaa
     4501 ggcagcgggt gcagatgccg gacat
//
LOCUS       DATGDI010000170         8755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_47207, whole genome shotgun sequence.
ACCESSION   DATGDI010000170 DATGDI010000000
VERSION     DATGDI010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 8755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8755
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47207"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1230
                     /locus_tag="VKO45_06440"
     CDS             172..1230
                     /locus_tag="VKO45_06440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMA05550.1"
                     /translation="MKVAIAGAGIAGGYLARLLEPRGIPVNLYDGTDHGTRCGCRSCG
                     WGVPENIRPYLARVGLDLDEYLLESMSSMNFDGLVAETPLSTLDKPRLLRDLAEGAPL
                     KGCNLVPEEADGYDVVVDATGVARSFLPPCRSDLTLPTLQHRVAVEPRDGAQIGIGVY
                     GNDVPGLGYLWIFPLGNDEYHIGIGGIGLVRHGILLERFYRESSERFSFTRLCACSGS
                     VRVASPYYSTPFFSRRIRGDGNPQLVVGVGESIGTVAPFTGEGIVHSLECARMLAESW
                     LDSEKYSRSVLARFAWMRKERETLDYLLSPEGRGGPRWRDRWRFYRNARRSDIRLPML
                     EAFRRMGSLSRWVDTREG"
     gene            complement(1257..1649)
                     /locus_tag="VKO45_06445"
     CDS             complement(1257..1649)
                     /locus_tag="VKO45_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA05551.1"
                     /translation="MKLLVTFSRRKGYEPIIAKVVKETGVLIRVDRAHIDSSGGEVLI
                     DVPQESCRLVSERMGSLGAVVKVLEHPVVRDESECVDCGACVSVCPREVFSFDGEWRL
                     VMQVERCVLCGKCIDACPHDALAQQVPG"
     gene            complement(1646..3154)
                     /locus_tag="VKO45_06450"
     CDS             complement(1646..3154)
                     /locus_tag="VKO45_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HMA05552.1"
                     /translation="MEKSIELINTRVREGNARVVTAEEMPAIVAELGEEGALAEVDVV
                     TTGTFGAMCSTGAFLNFGHSDPPIRMSQVFLNDVEAYGGVAAVDAYLGATQQSTTRGM
                     EYGGAHVLEDFVAGKRVELRATSQGTDCYPRRELTTELILDDLNQAIMVNPRNAYQRY
                     NAATNTTDRTLLTYMGMLLPDCGNVTYSGAGVLSPISNDPGFHVIGSGVPIFLAGAPG
                     MIIGEGTQHSPGKGFGTLMVTADMKEMSQEYLRAATMTGYGVTMYIGVGVPLPVIDLD
                     VVRSTAVTDADIGVNVIDYGIPSRDRPAIREVTYADLRSGEVEIRGEMVKTSSLSSFR
                     RARQVAGELKRWVEAGKVTLALPTRRIDARKLARPMRETARVPRVQDIMDHDVPSVSD
                     GEEIQEAARRLLKGDTNHLVVLDRDGILVGIITTYDLSKAIARPGRAKKVKDIMTTRV
                     ITTNPDEHIDIVARKLEQNNVSALPVVDPNRKVLGMLTAIDLGKLFGGRWLK"
     gene            3246..3953
                     /locus_tag="VKO45_06455"
     CDS             3246..3953
                     /locus_tag="VKO45_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HMA05553.1"
                     /translation="MPRDGLIRELEGIAPPGLADPEDAGRIGLVIEGRDEIGTICVAL
                     DVTPRVVARTVESGAGMLLVHHTPLWEPVTTLTGPLGMLLSPLLANRVNLYVMHTNFD
                     RGPGGINETLAELLGLTDTEPMEMGLIGNCSLDTAGIARRLGGPVRVWGRIERPSRLA
                     LVGGSGFDPERIYEAAYRGADAFLSAEMKHHVALSSPLPCIEATHYALEAPGMRRLAG
                     RMGWEFIDDPPGVTTIA"
     gene            3950..4285
                     /locus_tag="VKO45_06460"
     CDS             3950..4285
                     /locus_tag="VKO45_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05554.1"
                     /translation="MTDVWRLLGAERRLTPEIRRLIVERYGQRGEKALEIVDEGRVKK
                     YLDFFVVVGTSEEHVVEDEYCSCRDALYRRTGDCAHVLAVRIGAITGQYEEFSLWYQD
                     TWKRAEPGT"
     gene            4334..7072
                     /gene="alaS"
                     /locus_tag="VKO45_06465"
     CDS             4334..7072
                     /gene="alaS"
                     /locus_tag="VKO45_06465"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HMA05555.1"
                     /translation="MLEDEYLLDYFRDHGLERRVCTSCGGSFWTRDPERTLCGDAPCE
                     PYQFIGNPVLRPHTLDEMREAFLSFFERNGHARIERYPVVARWRDDIYLTIASIADFQ
                     PFVTSGVVPPPANPLTISQPCIRLNDLDSVGRSGRHLTLFEMMAHHAFNTDLHEVYWK
                     DRTVALCDEFLASAGADLVRVTYKEHPWIGGGNAGPSVEVLIGGLEVATLVFMNLGRI
                     RTGKPPVMLGGEAYYPMKTRIVDTGYGLERLAWASQGSPTVYDAVFPEILPRLMEAAG
                     LGEAYRDPGYRRILGESARFAGLMDIHGSNLLEMRKKVAASIGVPVGVLEEKILPVER
                     IYAIADHTRCLAYMLGDCIVPSNVREGYLARLVIRRTLRMMHDLGLDLDLADLVDRQM
                     EIAGKDGFVQEDQVVREIVGREVERYRQTLERGGRIVQRIAGTYRSRKEPIPLAEVIT
                     LYDSHGIPPETVKDLAEKEGVGAEVPDDFYSRIADLHAGEARSSEDPLEKYRERTREL
                     PPTRPLYYEAGGEMEFEATVLDNFDGFVVMDATLFYPEGGGQPSDTGTLVTAEGISRV
                     ESVVKLGDVILHQTTGGVIKKGERVKGKLDEERRLSLMRHHTGTHILLHAAKVVLGSH
                     IHQAGAQKGSESSRLDIRHFKHITPEELAKIEVEANRMVMMDIPVYIRWEERDKAEQK
                     FGFDLYQGGVPPGREIRVVQVDGEVQACAGTHCHSTGEVGAIKVIRVEHIQDGVERLE
                     FAAGIAALYHMQRVEKLLSEASGVLSVQPENLPTASARFFGEWKERGKEVERLRARLA
                     ELEARAAEPEMVKGVPVLVRRIDLAPRDLVALAREVAEKGGIAVLAGGSGPVHVAGAS
                     GMEGVSAAELVAKVCAALGGKGGGSPAKAQGGGPDAGAIGEALALAMKEIREALHD"
     gene            7159..7878
                     /locus_tag="VKO45_06470"
     CDS             7159..7878
                     /locus_tag="VKO45_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HMA05556.1"
                     /translation="MPDGVQPSIPGAVNPSAPDGGGVIILEPGDEKAQKIAKAIASRT
                     AGEILQLLKDGSHASTQIAESLKIPITTVQYHLENLVEAGIITVVEKRWSQKGREVKI
                     YGLRDRMLIVVPRGGDLKGILLRYGSLFAVVILASLVLTLLGPAFAPVAAPALQGALP
                     TPLPRAPPETGAGTVADHVLKEAANQTTAPAAIPVTGAIPGMETVPGVLPIPLVFFLG
                     GALVILVMLGYELWKYRERKG"
     gene            complement(7882..8484)
                     /gene="hisH"
                     /locus_tag="VKO45_06475"
     CDS             complement(7882..8484)
                     /gene="hisH"
                     /locus_tag="VKO45_06475"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HMA05557.1"
                     /translation="MKQVAVIDYGLGNLRSVMRGLERAGARVAITSDPREIEAADGIV
                     LPGVGAFRDGMEMLGDLRGTVTTLAGRVPLLGICLGMQMLAEWSEEHGLHEGLGLVPG
                     GVRRFPAGQGFKVPHMGWNTIRRTRDDPLLEGLKEEEYVYFVHSYYFDTPRGYAVTET
                     EYVRPFASSVCSGKVYGVQFHPEKSGEVGLRILRNFIGML"
     gene            8465..>8755
                     /locus_tag="VKO45_06480"
     CDS             8465..>8755
                     /locus_tag="VKO45_06480"
                     /inference="COORDINATES: protein motif:HMM:NF019469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HMA05558.1"
                     /translation="MTATCFTAISPPSLACTDGREADKKIGTGRPRGTHRSLFHRPGP
                     NGILSGGNTVDYLVTGGLVAILAIVLAGPFLVRRIEENLEAFLFACGVAALTV"
BASE COUNT         1451 a         2925 c         2905 g         1474 t
ORIGIN      
        1 gatcaacacg gtccacgggg caaggatcga ccagctctgc acccgtgccc tctactccgt
       61 cttccccgac ttcgagctcg cggcgaaccg gctcatgctg cggcaggtcg gggcttccct
      121 tgtccacgcg gcggccatca ccctcgtact cgcggccttc ggggggctcg gatgaaggtg
      181 gccatcgccg gggccgggat cgccggaggg tacctggcaa ggctcctcga accaaggggg
      241 atcccggtaa acctctacga cggcacggat cacggcaccc ggtgcgggtg ccggtcgtgc
      301 ggatgggggg tacccgagaa tatcaggccc tatctcgcca gggttggcct cgatctggac
      361 gagtacctgc tcgaatccat gtcctccatg aactttgacg gccttgtagc agaaaccccc
      421 ctctccaccc tcgataaacc ccgcctgctc cgggatctcg cggagggtgc ccccctgaag
      481 gggtgcaacc ttgtaccgga ggaggcagac ggctatgatg tcgtggtgga cgcgacggga
      541 gttgcccggt ccttcctgcc cccgtgcagg tctgacctga cgctgccgac gctgcagcac
      601 cgggtcgccg tcgaacctag ggacggggcg cagatcggga tcggggtgta cgggaacgat
      661 gtacccgggc tcgggtacct ctggatcttc cccctcggaa acgacgaata ccatatcgga
      721 atcggcggta tcggcctcgt ccgccacggg atcctcctcg agcggttcta ccgtgagtca
      781 tccgaaagat tttctttcac ccgcctctgc gcctgcagcg ggtctgtccg ggtcgcctcg
      841 ccctattact ccaccccgtt tttttcgagg aggatccggg gtgacgggaa tccccagctc
      901 gtcgtcggag tcggcgaatc catcgggacg gtggcgccct tcaccggaga ggggatcgtg
      961 cattccctgg agtgtgcccg catgctcgcg gaatcctggc tggattcgga aaaatattcc
     1021 cggtcggtcc tcgcacggtt cgcctggatg aggaaggagc gggagaccct cgattacctc
     1081 ctctcacccg agggaagggg cgggccgagg tggcgggacc ggtggaggtt ttaccgcaat
     1141 gcccgccgtt cggacatccg gctccccatg ctcgaggcct tcaggaggat gggaagcctg
     1201 tcccgctggg tggatacccg ggaagggtaa ccacggatcc ggaaaaaggg ggaatgtcag
     1261 ccggggacct gctgcgcgag ggcgtcgtgg gggcaggcgt cgatgcactt cccgcagagg
     1321 acgcaccgct ccacctgcat gacgagacgc cactccccgt cgaaggagaa gacctccctc
     1381 gggcagacgc tcacgcaggc cccgcagtct acgcactcgc tctcgtcgcg aacgacgggg
     1441 tgctcgagaa ccttcaccac ggccccgagc gagcccatcc tctccgagac gagccggcag
     1501 ctctcctggg gcacgtcgat gaggacctcg cctcccgacg aatcgatgtg ggcccggtcg
     1561 acccggatga ggacccccgt ctccttcacc accttcgcga tgatgggctc gtaccccttc
     1621 cggcgcgaga aggtcacgag gagcttcact tcagccacct cccgccgaag agcttcccga
     1681 ggtcgatggc cgtgagcatc ccgaggacct tccggttcgg gtccacgacg ggaagggcac
     1741 tcacgttgtt ctgctcgagt tttcttgcca cgatatcgat atgctcatcg gggttcgtcg
     1801 tgatcacccg ggtcgtcatg atgtccttca ccttcttggc ccggccgggc cgggcgatgg
     1861 ccttggagag gtcgtacgtc gtgatgattc ccacgagaat cccgtcccgg tcgagcacca
     1921 cgaggtggtt cgtgtccccc ttgagaagcc tgcgtgcggc ctcctggatc tcctcgccgt
     1981 cggagaccga tggcacatca tggtccatga tgtcctgcac ccggggcacc ctcgccgtct
     2041 cgcgcatggg gcgggcaagc ttccgcgcgt cgatcctccg ggtcgggagg gcgagggtca
     2101 ccttccctgc ctccacccac cgcttgagct ccccggcgac ctgcctcgcc cgccggaaac
     2161 tcgagagcga cgaggtcttc accatctccc cccggatctc cacctccccg gatctgaggt
     2221 ccgcgtacgt gacctcccgg atcgcgggcc ggtcgcggga gggaatgccg tagtcgatga
     2281 cgttcacccc gatgtcggca tcggtgaccg ccgtggatct gaccacgtcg aggtcgatga
     2341 ccgggagagg gaccccgacg ccgatgtaca tggtcactcc atagcccgtc atcgtcgccg
     2401 cccggaggta ctcctgcgac atctccttca tgtccgcggt caccatgagg gtcccgaagc
     2461 ccttccccgg agagtgctgg gtgccctcgc cgatgatcat ccccggtgcc ccggcgagga
     2521 agatggggac cccgctcccg atgacatgga aaccggggtc gttcgagatg ggggagagga
     2581 cccccgcccc cgagtaggtg acgttcccgc agtccgggag gagcatcccc atgtaggtga
     2641 ggagggtacg gtcggtggtg ttcgtggccg cgttgtaccg ctggtatgcg ttcctcgggt
     2701 tcaccatgat ggcctggttc aggtcgtcga ggatgagctc ggtggtgagc tcccgccggg
     2761 ggtagcagtc cgtcccctgg gaggtggccc ggagctcgac ccttttcccc gccacgaagt
     2821 cctcgaggac atgagctccc ccgtactcca tcccacgtgt ggtggactgc tgggtcgccc
     2881 cgaggtaggc gtccaccgcg gcgacccccc cgtaggcctc cacgtcgttc aggaagacct
     2941 gggacatccg gatgggcggg tcggagtggc cgaaattgag gaaggccccg gtggagcaca
     3001 tggccccgaa agtcccggtg gtgaccacgt ccacctcggc gagggccccc tcctcgccga
     3061 gttcggcgac gatggccggc atctcctcgg cggtgaccac ccgggcgttc ccttccctga
     3121 cccgggtgtt gatgagctcg atggacttct ccatgcccct aatgttttct gtgaataaat
     3181 atgaaggttt cgtattatcc agagtaataa gtgctccttt aaacacagac ggcatacggg
     3241 tacgtatgcc gcgcgacggc ctcatccggg agctcgaagg catcgccccc ccggggctcg
     3301 ccgatccgga ggacgcggga aggatcggcc tcgtcatcga gggacgtgat gagatcggta
     3361 ccatctgtgt ggccctggac gttacccccc gggtcgttgc ccggacggtg gaatccggcg
     3421 ccgggatgct cctcgtccac cacacccccc tctgggagcc ggtcaccacc ctcaccggcc
     3481 ccctcgggat gctcctctcc cccctcctcg caaaccgggt gaacctctat gtcatgcaca
     3541 cgaacttcga ccggggcccc ggcggcatca acgagaccct cgccgaactc ctcggtctca
     3601 cggacaccga gccgatggaa atggggctca tcgggaactg ctccctcgat accgccggga
     3661 tcgcccgcag gctcggcggg ccggtccggg tctggggcag gatcgaacgt ccctcccgcc
     3721 tcgccctcgt cggggggagc ggctttgacc ccgaacggat ctacgaggcc gcgtaccggg
     3781 gggccgatgc cttcctttcc gccgagatga agcatcacgt cgccctctcc tctcccctcc
     3841 cctgcatcga ggccacccac tatgcgctgg aggcgccggg catgagaagg ctcgccgggc
     3901 ggatggggtg ggagttcatc gacgatcccc ccggggtcac caccatcgca tgaccgacgt
     3961 ctggaggctt ctcggagcgg agagacggct caccccggag atccggaggc tcatcgtgga
     4021 gcgctacggc cagagagggg agaaggccct cgagatcgtg gacgaggggc gggtgaagaa
     4081 atacctggac ttcttcgtcg tggtcgggac ctcggaggag cacgtggtcg aggacgagta
     4141 ctgcagctgc cgggatgccc tctaccggcg cacgggtgac tgcgcccacg tgctcgccgt
     4201 gaggatcggg gccatcaccg ggcagtacga ggagttctcc ctctggtacc aggatacatg
     4261 gaagagggcg gaacccggga catgaagttt ttatcggaac atcacgaaga tcggtacaga
     4321 gaggtggggc cgcatgctcg aggatgaata cctgctcgac tatttccggg atcacgggct
     4381 cgagaggagg gtctgtacct cctgcggggg cagcttctgg acccgtgacc ccgaaaggac
     4441 cctgtgcggg gacgccccct gcgagcccta ccagttcatc gggaacccgg tcctccggcc
     4501 ccacaccctc gacgagatgc gggaggcctt cctctccttc ttcgagagga acggccatgc
     4561 ccgtatcgag cggtaccccg tggtggcccg gtggagggac gacatctacc tcaccatcgc
     4621 ctccatcgcc gatttccagc ccttcgtcac ctcgggcgtc gtcccgcctc ccgcaaaccc
     4681 cctcaccatc tcccagccct gcatccggct gaacgatctc gactcggtgg ggcgctcggg
     4741 gaggcacctg accctcttcg agatgatggc ccaccatgcc ttcaacaccg acctccacga
     4801 ggtgtactgg aaggaccgga ccgtcgccct ctgcgacgag ttcctggcat ccgccggtgc
     4861 cgacctcgtg agggtcacgt acaaggagca cccctggatc ggcggcggaa acgcgggccc
     4921 gagcgtcgag gtgctcatcg gggggctcga ggtggcgacc ctcgtgttca tgaacctagg
     4981 aaggatccgg accgggaagc ccccggtgat gctgggcggt gaggcctact acccgatgaa
     5041 gacccggatc gtggacaccg gctacggcct cgaacggctc gcctgggcct cccagggttc
     5101 ccccacggtg tatgacgcgg tcttccccga gatcctcccc cggctcatgg aagcggcggg
     5161 gcttggcgag gcctaccggg atcccgggta ccggaggatc ctcggggaga gcgccaggtt
     5221 cgcgggcctc atggacatcc atgggtcgaa tctcctcgag atgcggaaga aggtcgcggc
     5281 ctccatcggg gttcctgtcg gggtcctcga ggagaagatc ctccccgtcg agcggatcta
     5341 tgccatcgcc gaccacaccc gctgcctcgc ctacatgctc ggggactgca tcgtcccctc
     5401 caatgtccgg gaggggtacc tcgcccggct cgtgattagg cggaccctcc ggatgatgca
     5461 cgacctcggt ctcgatctcg acctcgcgga tctcgtggac cggcagatgg agatcgcggg
     5521 gaaggacggg tttgtccagg aggatcaggt tgtccgggag atcgtcgggc gggaggtgga
     5581 gcgctaccgc cagaccctgg agcgcggggg aaggatcgtc cagcggatcg cgggcaccta
     5641 ccggtcgagg aaggagccca tccccctcgc agaggtcatc accctctacg actcccacgg
     5701 catccccccc gagaccgtga aggatctcgc ggagaaggag ggggtggggg ccgaagtccc
     5761 ggacgacttc tattcccgga tcgccgatct ccacgccggc gaggcccgtt cctccgagga
     5821 ccctctggag aagtaccggg aaaggacgag ggagctcccc ccgacccgtc ccctctacta
     5881 cgaggccggc ggtgaaatgg agttcgaggc aacggtcctc gataacttcg acggcttcgt
     5941 ggtgatggac gctaccctct tctaccccga gggcgggggc cagccctcgg acaccgggac
     6001 actcgtcacc gccgagggga tctcccgggt cgagtccgtg gtgaaactcg gggatgtcat
     6061 cctccaccag acgacgggcg gggtcatcaa gaagggggag agggtgaagg ggaagctcga
     6121 cgaggagcgg cgcctctccc tcatgcgtca ccacacgggg acccacatcc tcctccacgc
     6181 ggccaaggtc gtccttggga gccacatcca ccaggcgggg gcccagaagg ggagcgagtc
     6241 ctcccggctc gacatccggc acttcaagca catcaccccc gaggagctcg cgaagatcga
     6301 ggtcgaggcg aaccggatgg tcatgatgga catcccggtc tacatccggt gggaggagag
     6361 ggacaaggcg gagcagaagt tcggcttcga cctctaccag gggggcgtcc cgccgggaag
     6421 ggagatccgg gtggtgcagg tggacgggga ggtgcaggcc tgcgccggga cccactgcca
     6481 ctcgacgggt gaggtggggg ccatcaaggt catccgggtc gagcacatcc aggacggggt
     6541 ggagcggctc gagttcgctg ccggcatcgc cgccctctac cacatgcagc gggtggagaa
     6601 gctcctctcc gaggcctcgg gggtcctctc ggtccagccc gagaacctcc ccaccgcctc
     6661 ggcccggttc ttcggcgagt ggaaggagcg gggcaaggag gtcgagcgcc tccgggcgag
     6721 gctcgccgag ctcgaggccc gggccgcgga accggagatg gtgaaggggg tcccggtcct
     6781 cgtgcggcgc atcgacctcg ccccgaggga cctcgtcgcc ctcgcccgcg aggtcgcgga
     6841 gaagggcggg atcgccgtcc tcgccggcgg gagcggaccg gtccacgtgg ccggtgcctc
     6901 ggggatggag ggggtaagcg ccgccgagct cgtcgcgaag gtctgcgcgg ccctcggggg
     6961 aaagggcggc gggagcccgg caaaggccca gggcggcggg ccggatgccg gtgccatcgg
     7021 cgaggccctt gctctcgcga tgaaggagat cagggaggcg ctgcatgact gatgccatgc
     7081 agaggtccat actcaaccgt atggacccct cccgcctgcc ggctgggagg ccctccgcct
     7141 tgcggcagga ggcgctccgt gcctgatggc gtgcagccct caatacctgg cgccgtgaat
     7201 ccctctgcac cggatggcgg cggtgtcatc atccttgagc ccggcgacga gaaggcccag
     7261 aagatcgcga aggccatcgc gagccggacc gcgggggaga tcctccagct cctgaaggac
     7321 gggagccatg catccaccca gatcgccgaa tccctgaaaa ttcccatcac cacggtccag
     7381 taccacctgg agaacctggt ggaggcgggg atcatcaccg tcgttgagaa gcggtggtcc
     7441 cagaagggcc gcgaggtgaa gatctacggc ctccgggacc ggatgctcat cgtcgtgccc
     7501 cggggggggg atctgaaggg catccttctc cggtacggct ccctctttgc ggtggtcatc
     7561 ctcgcgagcc tcgtcctcac ccttctcggg ccggcctttg caccggtggc ggcacccgcc
     7621 ctccagggcg cgctcccgac ccctctcccc cgggctcctc cggaaacggg ggcaggcacg
     7681 gtggcggacc atgtgctcaa ggaggccgcg aaccagacga cagcgccggc ggcaatccct
     7741 gtcaccgggg caatccccgg gatggaaacg gttcccggcg tccttccaat ccccctcgtc
     7801 ttcttcctcg gaggggcgct cgtgatcctc gtcatgctcg ggtacgagct gtggaagtac
     7861 agggaaagga aggggtaggg ctcacagcat cccgatgaag ttccggagga tgcggagccc
     7921 cacctcgccg ctcttttcag ggtggaactg caccccgtag accttcccac tgcagacgga
     7981 tgaggcgaag ggcctgacgt attcggtctc ggtgactgca tacccgcggg gtgtgtcgaa
     8041 gtagtaggag tgcacgaagt acacgtactc ctcctccttc aatccctcga ggaggggatc
     8101 gtcgcgggtc ctcctgatgg tgttccatcc catgtggggg accttgaagc cctgcccggc
     8161 agggaacctc cgcacgccgc cggggacgag gcccaggccc tcgtggaggc cgtgctcctc
     8221 gctccattcg gcaagcatct gcatccccag gcagatgccg aggaggggga ccctgccggc
     8281 gagggtggtg accgtgcccc ggagatcgcc gagcatctcc atgccgtccc ggaaggcgcc
     8341 gacgccgggg aggacgatcc cgtccgccgc ctcgatctcc cggggatcgg aggtgatggc
     8401 gacccgggcg cccgcccgct cgaggccccg catcacactc ctcaggttcc cgagcccgta
     8461 gtcgatgacg gcgacctgct tcacggcgat ttctccgccc tccctcgcat gtacggatgg
     8521 gagagaggcc gataaaaaga ttgggacagg aaggccccgg gggacccatc ggtccctttt
     8581 tcaccgcccg ggtccaaacg gtatcctgtc cggaggtaac acggtggatt acctagtcac
     8641 gggcggtctc gtcgccatcc ttgccattgt gctcgccggc ccgttccttg tccgccggat
     8701 cgaggagaac ctggaggcct tcctcttcgc ctgcggggtg gccgccctca cggtc
//
LOCUS       DATGDI010000171         1753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_425338, whole genome shotgun sequence.
ACCESSION   DATGDI010000171 DATGDI010000000
VERSION     DATGDI010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425338"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..448)
                     /locus_tag="VKO45_06485"
     CDS             complement(44..448)
                     /locus_tag="VKO45_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05559.1"
                     /translation="MSGITRLVTALQNIPGVRSAVAESDTVRFFVDDGNLARVIRELQ
                     QKVDVSPVAGRIFFRVAFDENDMVGFEVVAGRPADIDGFAKALTGESRPKFRLTNGCP
                     RCGDKGMQYIRKKREYICKVCGHTIDEELVER"
     gene            534..1427
                     /locus_tag="VKO45_06490"
     CDS             534..1427
                     /locus_tag="VKO45_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05560.1"
                     /translation="MKPQFILLLVSALLLAGCLGGGPPPQGNTTYTPPQLKYLLLDHY
                     GENQFFYCDPDYYPVGRGDELERAIAVFPVIEKETDVFAAITARKGFQPPYSNDTILT
                     IYREYKKLNAIPLNPLATDVYSFNLRVGTTGEGKLVTGAIRRDGVILGEQFNNAVLTC
                     PICLAGDTLIDTPDGPVPVKDLREGMPVWTPGPKGGRVAVPLLRTAKTRVPPTHRLVH
                     LMLSDGRELSASPGHPTLDGRALGTLSVGDDLDGSGVTRAELVPMTEEFTCDILPAGG
                     TGGYWANGIPLRSTLFPAPPG"
     gene            1540..>1753
                     /locus_tag="VKO45_06495"
     CDS             1540..>1753
                     /locus_tag="VKO45_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05561.1"
                     /translation="MVRRTTPVALDQKEDLFRKGREIADALKEVPEVAATEYRAGGRG
                     VRVIFAVPDRQAGDIVRRLLEKFRITP"
BASE COUNT          357 a          556 c          501 g          339 t
ORIGIN      
        1 agaggaaaaa aatcaaggga tgaaaggaag ttcatggaac catttatctt tccaccagct
       61 cttcgtcgat ggtgtgcccg cacaccttgc agatgtactc ccttttcttc ctgatgtact
      121 gcatcccctt gtcaccgcac ctcgggcagc cgttcgtgag cctgaatttc ggcctcgatt
      181 cgccggtcag cgccttggcg aaaccgtcga tgtccgcggg acgaccggcc acgacctcaa
      241 agccgaccat gtcattctca tcaaaggcta cccggaagaa aatcctgccc gcgaccgggg
      301 agacatcgac cttttgctgg agctcacgga tgacccgggc gagatttccg tcatccacga
      361 aaaacctgac cgtgtctgat tcagcgaccg ctgatctgac gccggggatg ttctgtaatg
      421 ccgtcacgag tcgggtgatg ccgctcataa ggcaaggttt gcgctgattc tatttaaggg
      481 atggaacagg ggtcatgcga atccttttaa accgttacgg taccccctca ggcatgaagc
      541 cccaattcat cctcctcctg gtatccgccc tcctcctcgc cggttgtctc gggggtggac
      601 ctccgcccca ggggaacacg acctataccc ctccccagct gaaatacctc ctcctcgacc
      661 attacgggga aaaccagttc ttctactgcg accccgacta ttacccggtg ggccggggtg
      721 acgagctgga acgtgccatc gcggtattcc ccgtcatcga gaaagagaca gacgtcttcg
      781 cagccatcac agcccggaag ggattccagc cgccctactc caatgacacc atcctcacca
      841 tctaccgcga gtacaagaaa ctcaatgcca ttcccctcaa tcccctggcg acagatgtct
      901 attccttcaa cctgcgggta ggtacaacag gggagggaaa gctggtgacg ggcgccatcc
      961 ggagggatgg tgtcattctt ggcgagcaat tcaacaacgc cgttctcacc tgtcccatct
     1021 gcctcgcggg tgataccctc atcgacacgc ctgatggccc cgtaccggtg aaggatctcc
     1081 gtgagggaat gcccgtgtgg acccccggtc cgaaaggagg ccgggtggcc gttccccttc
     1141 tccggacggc aaagacccgg gtcccgccca cccaccgcct cgtccacctc atgctctccg
     1201 acggacggga actctccgcc tcccccggcc atccgacgct ggacggaagg gccctgggaa
     1261 ccctgtctgt cggggacgat ctggacgggt cgggagtgac ccgggctgaa ctcgtcccca
     1321 tgacggaaga gttcacctgc gatatcctcc ctgccggcgg aaccggcggg tactgggcga
     1381 atggaatacc cctgaggagc accctttttc cagctcctcc cgggtaagaa tcctgaaagg
     1441 accacatccg gagaccaacc ggggggaagc ccgggcagga cctcggcgtg aggatcatct
     1501 ttatctctcc gagcccccat cgcatgccgg tggaggaata tggttcgtcg gacgacaccg
     1561 gtggctttgg atcagaagga ggatctgttc aggaagggga gggagatcgc cgatgccctg
     1621 aaggaggtgc ccgaggtggc cgcgactgag taccgggcag gcggaagggg tgtccgggtc
     1681 atctttgcgg tgcccgacag gcaggcgggg gatatcgtcc ggcgtctcct cgagaagttc
     1741 aggatcacac ccg
//
LOCUS       DATGDI010000172         2899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_279921, whole genome shotgun sequence.
ACCESSION   DATGDI010000172 DATGDI010000000
VERSION     DATGDI010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279921"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..727
                     /locus_tag="VKO45_06500"
     CDS             395..727
                     /locus_tag="VKO45_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HMA05562.1"
                     /translation="MVPGRKLTKEELIRAIRQDLAAEHEAVHLYLAHADATDHPLAKA
                     VLTDIANEERVHAGEFARLIALLTGDEDSFLAKGAKEVDDTALGLGGKGSTPAQGTGP
                     KTVGSLRE"
     gene            743..1531
                     /locus_tag="VKO45_06505"
     CDS             743..1531
                     /locus_tag="VKO45_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HMA05563.1"
                     /translation="MDGTYLGRGDAPISGETWDLLDATMIEAARGILAGRRLLGISGP
                     HGLGLKAVPLSDSRGRKGFVTSELLPVPLIQVPFCLGKRDIAAYERDRVAFDTCAVAT
                     AACAAATAEDALIFQGSPGIAGLLSAKGSPTVKISPWETVGAAADEVIGAVTALDDGG
                     FHGPYSLGLAPARFNLLLRRYPQGEGTELDHLRTIVTGGIAKAPVLRDGGVLIASGRQ
                     YAELVIGQDMSVGFIGPAEENLEFSISESLVPLIHTPEALCILK"
     gene            complement(1548..2657)
                     /locus_tag="VKO45_06510"
     CDS             complement(1548..2657)
                     /locus_tag="VKO45_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HMA05564.1"
                     /translation="MVDYLVTGGLVAILAIVLAGPFLARRIEENLEAFLFACGVAALT
                     VAGLASIEGVPTGWSAAIVEEALATPVYIGSFLGIPVGIVQIVLLVGFLIYLGHRPLE
                     RAIQRVVDTVPLRVLAFLLVVALALLSSVISAILASILLIEVLCALPLPKEPKVRLAV
                     LACFAIGLGAALTPLGEPLSTIAISKLSGPPYHAGFGFLANLLGIYVLPGILAFGFFA
                     AFTVREVKGGGEPLLCQVERGTLKEVVLRAAKVYLFIMALVFLGEGFKPLILQFVAGI
                     PADGLFWVNMVSAVLDNATLTAAEIGPYLSVIQIRAALMGLLVSGGMLIPGNIPNIIA
                     AGKLGITSREWGRVGIPLGFVAMAVYFVVLFFPGM"
     gene            2793..>2899
                     /gene="hisH"
                     /locus_tag="VKO45_06515"
     CDS             2793..>2899
                     /gene="hisH"
                     /locus_tag="VKO45_06515"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286020.1"
                     /note="with HisF IGPS catalyzes the conversion of
                     phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide
                     ribonucleotide phosphate and glutamine to
                     imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamide ribonucleotide, and
                     glutamate in histidine biosynthesis; the HisH subunit
                     provides the glutamine amidotransferase activity that
                     produces the ammonia necessary to HisF for the synthesis
                     of imidazole-glycerol phosphate and
                     5-aminoimidazol-4-carboxamide ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HMA05565.1"
                     /translation="MKQVAVIDYGLGNLRSVMRGLERAGARVAITSDPR"
BASE COUNT          569 a          886 c         1009 g          435 t
ORIGIN      
        1 gtgagccgcg gaagcgactt ttccgctgaa aaggagctta agcagcgcga agggcagcgc
       61 gaaggcgctg cggcaaacgg ctttttaggt aaaaagacgt tccgcggcgt gagccgcggg
      121 tgagactttt ccgctgaaaa ggagcttaag cagcgcaaag gcgctgcggc cgacgacttt
      181 cccaagaaaa gacgtcccgg ggcgctgagc tgcagagagg tttaccccgg aaaaggggca
      241 gcggcgataa agcggcccgt cggggctccg gtttccttta atcctttcaa tccatgccgt
      301 tcaccgaaag gtatatcaat cctccttccg agggtgcacc cggtaaaacc gattggagtt
      361 gcgtatgccc gaattcctga acccttttac aggcgtggta cccggacgga agctcaccaa
      421 ggaggaactg atccgggcca tccgccagga cctcgccgcc gagcatgagg cggtccacct
      481 gtacctggcc catgcagatg ccaccgacca ccccctggca aaggcggtgc tcaccgatat
      541 cgcgaacgag gagcgtgtcc atgccgggga gttcgcccgg ctcatcgccc tcctgaccgg
      601 cgacgaggac agcttcctcg cgaagggggc aaaagaggtg gacgataccg ccctcggcct
      661 cggggggaag ggaagtaccc cggcccaggg aaccgggccg aagaccgtcg gttccctcag
      721 ggagtgaccg gaggagagaa tcatggacgg aacttacctc ggacgcgggg atgcacccat
      781 cagcggggag acctgggatc tcctcgatgc cacgatgata gaggcggcga gggggatcct
      841 tgccggaagg cgcctcctcg ggatcagcgg cccccatggc ctcggtctga aggcggtgcc
      901 cctctcggac agcaggggaa ggaaggggtt cgtcacgagc gagctcctgc cggtgcccct
      961 catccaggtc ccgttctgcc tgggaaagag ggacatcgcc gcttacgaac gggaccgggt
     1021 cgccttcgac acctgcgccg tggcaacggc ggcctgtgcc gccgcgaccg cggaggacgc
     1081 gctgatcttc caggggagcc ccggcatcgc gggactcctc tcggcaaagg ggtcaccgac
     1141 ggtgaagatc tccccgtggg agaccgtcgg ggctgccgcc gatgaggtca tcggcgcggt
     1201 cacggctctc gatgacgggg gcttccacgg gccgtactcc ctgggactcg cgcccgcccg
     1261 ttttaacctc cttctccggc ggtaccccca gggggagggg accgagctcg atcacctccg
     1321 taccatcgtg accggcggga tcgccaaggc cccggtgctg agggacggag gggttctcat
     1381 cgcatcgggg aggcagtacg cggaactcgt catcggccag gacatgtccg tcgggttcat
     1441 cggtccggcc gaggagaacc tggagttctc catctcagag agcctggttc ccctgatcca
     1501 cacgccggag gccctctgta tcctgaagtg agggaatatt ctctttttca catcccgggg
     1561 aagaacagga cgacgaagta caccgccatg gcgacgaagc cgagggggat gccgaccctg
     1621 ccccactccc ggctcgtgat cccgagctta cctgcggcga tgatgttcgg gatgttcccg
     1681 ggaatgagca tcccccccga gacgaggagg cccatgaggg ccgcccggat ctggatgacc
     1741 gagaggtacg gtcctatttc ggccgcggtg agggtggcgt tatcgaggac cgccgagacc
     1801 atgttcaccc agaagaggcc gtccgccggg atcccggcca cgaactggag gatgaggggc
     1861 ttgaacccct cgccgaggaa tacgagggcc atgatgaaga gatagacctt cgccgcccgg
     1921 aggacgacct ccttcagggt gcctcgttcc acctggcaga gaaggggctc gcccccgccc
     1981 ttcacctccc tcaccgtgaa ggcggcgaag aatccgaagg caaggatgcc cgggaggacg
     2041 tagataccga ggaggtttgc gaggaagccg aagccggcgt gatagggtgg tcccgagagc
     2101 ttcgagatgg cgatggtgga gaggggctcc ccgagggggg tgagggccgc cccgaggccg
     2161 atggcaaagc aggccaggac ggcgagccgt actttgggtt cctttgggag ggggagggcg
     2221 cagagcacct cgatgaggag gatggaggcg aggatggccg agatgacgct cgagagaagc
     2281 gcgagggcaa cgacgaggag gaaggcgagt accctcaacg gcacggtgtc aaccacccgc
     2341 tggatcgccc gctccagggg gcggtggccc aggtagatga ggaacccgac gagaaggacg
     2401 atctggacga tgccgacggg gatgccgagg aagctcccga tgtacaccgg tgtcgcgagg
     2461 gcctcctcca cgatggccgc agaccacccg gtcgggaccc cctcgatgga ggcaaggccg
     2521 gcgaccgtga gggcggccac cccgcaggcg aagaggaagg cctccaggtt ctcctcgatc
     2581 cggcgggcca ggaacgggcc ggcgagcacg atggcaagga tggcgacgag gccgcccgtg
     2641 acgaggtaat ccaccatgtt cctcccgaca ggataccgtt gggacccggg cggtgaaaaa
     2701 gagaccgatt ggttccccgg ggccttcctg tcccaatctt tttatcggcc cctctcccat
     2761 ccgtacatgc gagggagggc ggagaaatcg cagtgaagca ggtcgccgtc atcgactacg
     2821 ggctcgggaa cctgaggagt gtaatgcggg gcctcgagcg ggcgggcgcc cgggtcgcca
     2881 tcacctccga tccccggga
//
LOCUS       DATGDI010000173         4173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_105376, whole genome shotgun sequence.
ACCESSION   DATGDI010000173 DATGDI010000000
VERSION     DATGDI010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105376"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            477..3593
                     /locus_tag="VKO45_06520"
     CDS             477..3593
                     /locus_tag="VKO45_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05566.1"
                     /translation="MKTIILIMLAALSLGAAVSAAYPVELTVTSDHVWMVADNRDTVT
                     ITANVTYGTGELAGQPLEGASLSLTVGSPWKLKDSSLVTDQRGIATTTLLPTTKSGTA
                     TITVTAAALIPNESGEYVSYSTTKTLSQDIDHTGPASAATYYNGQVQVRTATPIAVLV
                     RDRYGNPVDNRNVVETVKFDASSRGISGFQSGGSSVKSITVPVNASGYATVLYFVDPP
                     GTNSITITPPSPLPMKIVTITGLSQARPFSAASLVSPGGTPYPYTTVKTGQFTIGFTF
                     LDEFGYPTMNQPVEVTTSVPGESMSLTTNKNGMVIITYGPKDVAGVYILTATAANNHS
                     VTTSPKVEFVSGTATDALLTASPLTMASRDVKDDITSLLTMRVTDSKGNPVGGETVRF
                     RFSSLSTSPLANQTMSPVLENGVDSTNSTGTDIVVTSNMEGEAVATFHPGAFSTDFSA
                     PGYNATATGSAVVEARWSSVTRQLTLSYLNYPYLTVQSWVTPATVRVNETVDVMVKVT
                     GDGWALQPKPIDVMLLSDRSGSMSEDLPDRAVSVIGASQIFSSKLDYSKDRLGLVSFG
                     GKGRTDVTASSNTGRDGDSSDDAAYALANYPGNNRTYSDYATTDLNLTSSQAAINNEI
                     PRLVPGGYTPMRYVLKVAIDQMMSRGRPNVVKALILLSDGDYNWYGDPLAVRSYSSSW
                     PTTPSSYSDLDTRWMKFAEITNNTQQNMAQYARANNIKIFTIGFAQGISSGGQASLQS
                     LATQTGGRYYYAPTADMLAGVYTDIAGSLKDTAGVNTGMNLSFQSITVNGNVTPGDQV
                     YSYVFIDGHSTLVDSWNTSLAHLPGYPASYNSTDQWITRNRNLNFSIGTVQLGQVWQS
                     TITLRVLKDGSISVFDPASKISMQDSTFPLKIPEAYFTSLPNNSANTLMIPYHLSIEN
                     LQLTNAGSLTSADIRWSLTYNGLESISEDVMIAEYGTMRWDHLFQPLRTVSNVTTTDA
                     ASIPLAGRGTGYYTIRVDADADDANPASTFLEVFLSDAGVRPLNPGETPLPGVGATPV
                     PPPKIKIS"
BASE COUNT          938 a         1310 c         1108 g          817 t
ORIGIN      
        1 gagccgtacg ccgttcctcc gactatcgac gatcccgaca tcctcgacga gatcagggag
       61 agccttgccg cgctggggta cccccggggg aacccctgat cccggaagga gtctttcgaa
      121 agccccccga ggcccacatc aaaggtgggg aactccgcat tctgccggag gatgggatct
      181 gcgcccgtac ccatccgacg aatgcaaact tcaatcttgt tttactttat aaggataacc
      241 cctcagaagt caatgattct agatttattt tgaagatctc ctggattgga cgcatatagg
      301 ttcaggaggc tgatccttca atgaaaataa tgaattttgc aggtctgtgt ttgattatac
      361 cggttattat cgtttagaaa agcagtcaca atgatttaat gacatattgt taagtagaaa
      421 taattatata gtattgcaaa taactgaatg tagatcgaaa gaagggggat gaaacgatga
      481 aaaccatcat cctgatcatg ctcgcggccc tttccctcgg agctgcggtt tctgccgcat
      541 atcccgtgga gctcacggtc acgagcgacc atgtatggat ggtggcggac aacagggaca
      601 ccgtcaccat cacggccaat gtgacctacg ggaccgggga attggccggg cagcccctcg
      661 agggcgccag tctcagcctc acggtcggtt cgccgtggaa actgaaggat tcctccctcg
      721 ttaccgacca gagggggatc gccaccacga ccctccttcc aaccacaaag agcggcaccg
      781 ccaccatcac cgtgaccgcg gccgccctga tccccaacga gtccggggaa tacgtcagtt
      841 acagcacgac gaaaaccctc agccaggaca tcgaccatac cggccccgca tccgcggcaa
      901 cctactacaa cggccaggtg caggtgagga ccgcgacccc catcgccgtc ctcgtcaggg
      961 acagatacgg gaacccggtg gataaccgga atgtcgtgga gaccgtgaaa ttcgatgcat
     1021 cctcccgggg tatttccggt tttcaatccg gtggctcctc ggtaaaatcc atcaccgtgc
     1081 ccgtgaatgc ctcgggatac gcgaccgtcc tgtatttcgt ggatccgccg ggcaccaatt
     1141 ccatcaccat cacgcccccg tcgccccttc cgatgaaaat cgtaaccatt accggactat
     1201 cccaggcccg gcccttctcg gccgcgtccc tcgtatcccc ggggggtacc ccctacccct
     1261 atacgacggt gaagaccggg cagtttacca tcggtttcac cttccttgac gagttcgggt
     1321 atcccaccat gaaccagccg gtggaagtca cgacaagcgt gcccggggag tcgatgtccc
     1381 tcaccacgaa caagaacggg atggtgatca tcacctacgg gcccaaggac gtcgccggcg
     1441 tctacatcct caccgcgacg gcggcgaaca accactcggt aacgacctcc ccgaaggtgg
     1501 aattcgtcag cgggaccgcg acggatgccc tccttaccgc gagccccctc accatggcga
     1561 gccgggacgt gaaggacgat atcacctccc tcctcaccat gagagtaacg gactcgaagg
     1621 ggaacccggt cgggggagag acggtcaggt tcaggttcag ctcgctctcc acgagcccgc
     1681 tcgccaacca gaccatgtcc ccggtcctcg agaacggggt cgacagcacg aactccaccg
     1741 gaacggacat cgtcgtgacc tcgaacatgg aaggggaggc cgtcgccacc ttccaccccg
     1801 gcgccttcag caccgacttc agcgccccgg gctacaatgc caccgcgacc gggagcgccg
     1861 tggtcgaggc ccggtggtcc tcggtcacga gacagctcac cctctcgtac ctgaattatc
     1921 cctacctcac ggtacagagc tgggtcaccc ccgcgacggt ccgggtgaac gagacggtgg
     1981 atgtcatggt gaaggtgacc ggggacgggt gggccctcca gccgaagccc atcgacgtga
     2041 tgctcctgtc cgaccggtcg ggatcgatgt ccgaggatct cccggatcgg gccgtcagtg
     2101 tcatcggggc atcccagatc ttcagttcca agctcgatta ctccaaggac cggctcgggc
     2161 tcgtctcctt cggggggaag gggaggacgg acgtcacggc gagctccaac acgggcaggg
     2221 acggcgattc cagcgatgac gccgcatacg ccctcgcgaa ttacccggga aataaccgga
     2281 cgtacagcga ttatgccacc acggacctga accttacctc cagccaggca gccatcaaca
     2341 acgagatccc ccgtctcgtg ccgggcgggt acactcccat gcgatatgtc ctgaaggtgg
     2401 ccatcgatca gatgatgagc cggggcaggc ccaacgtggt gaaggccctc atcctgcttt
     2461 ccgacggcga ttataactgg tacggcgatc ccctggcggt gagatcctac tcctccagct
     2521 ggcccaccac cccgtcgagt tacagcgatc tcgacacccg ttggatgaag ttcgctgaga
     2581 tcacgaacaa cacccagcag aacatggccc agtatgcaag ggcgaataac atcaagatct
     2641 tcaccatcgg ctttgcccag ggcatttcct cggggggcca ggcctccctg cagtccctcg
     2701 cgacccagac cgggggcagg tactactatg cgccgacggc cgacatgctc gcgggagtct
     2761 acaccgacat cgcggggtcc ctcaaggaca cggccggggt gaataccggg atgaacctct
     2821 ccttccagag cattacggtg aacggcaacg taacccccgg tgaccaggtg tacagttacg
     2881 tcttcatcga cggacactcc accctggtcg actcctggaa tacctccctc gcccacctcc
     2941 cgggataccc ggcgagctac aattccaccg accaatggat cacccggaac cggaatctca
     3001 atttcagcat cgggaccgtc cagctcggcc aggtgtggca gtccaccatc acgctccggg
     3061 tactcaagga cgggagcatc agcgtcttcg accctgcctc gaagatcagc atgcaggaca
     3121 gcaccttccc cctgaagatc ccggaggcct acttcacgtc gctgcccaac aattcggcga
     3181 acaccctcat gataccttac cacctgtcca tcgaaaatct ccagctgacg aatgccggct
     3241 cgctgacgag tgccgatatc cggtggagcc tcacctataa tggcctggaa tccatctcgg
     3301 aggatgtcat gatcgcagag tacgggacca tgagatggga tcacctcttc cagccgctcc
     3361 ggacggtttc caacgtcacg accaccgacg cggcgagcat accccttgcg ggccgcggga
     3421 cgggatacta caccatcagg gtcgatgcgg atgccgatga tgccaacccc gccagcacct
     3481 tcctggaggt gttcctctcc gatgccggtg tccggcccct gaatcccggt gaaacacccc
     3541 tccccggcgt cggggccacc ccggttccac ctccgaagat caagataagc tgaatcaggg
     3601 gcagtgaccc cgatctcttt ttccgtatag ctgagaggag ccagaacaga ggaggatcgg
     3661 tgggcgggtt attcggggag gatcctctcc agctctcctc actccccccc cctgattctt
     3721 ctctgatggg ttcattcgag ttgcggggga tccggtttcc cttcacgagg aatgcacctg
     3781 gcagggacgc cgatcgcatg gggtcgtgat gggaactgcc gcatccgacc tccgccggtt
     3841 gatctcttcg atcttgttgt acattataaa tattacccac tgtaaatcaa ggattcatga
     3901 ttaatcctgg tattttatcg atttattggt ggaatgaatg atgccattaa tcctcaagac
     3961 acaataacga atttaaacgg ttatatcctg tttttacagt taataacaat ttaaaatcat
     4021 tattattgaa gatatccagc gaatcatgta acagaaatct atatatacaa ttgaaaacaa
     4081 ttaaaatatt aattgacggg gatgagataa tgagaaccat cgtcatgatc atgctcgtgg
     4141 ccctctccct gggagctgcg gtttccgccg cat
//
LOCUS       DATGDI010000174         4013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_366945, whole genome shotgun sequence.
ACCESSION   DATGDI010000174 DATGDI010000000
VERSION     DATGDI010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366945"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..238
                     /locus_tag="VKO45_06525"
     tRNA            166..238
                     /locus_tag="VKO45_06525"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:200..202,aa:Gln,seq:ttg)
     gene            complement(259..699)
                     /locus_tag="VKO45_06530"
     CDS             complement(259..699)
                     /locus_tag="VKO45_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HMA05567.1"
                     /translation="MDGFMQAAIGEARRGLAEGGIPIGSVLVIGGRIVGQGHNRRVQQ
                     GSPTLHAEMDCLEHAGRLRPSDYRRAILYSTLSPCDMCSGAALLYGIPKIVIGENRTF
                     RGPEEYLRSRGVELVILDDGECIRMMRDFIAARPDLWKEDIGEE"
     gene            838..1329
                     /locus_tag="VKO45_06535"
     CDS             838..1329
                     /locus_tag="VKO45_06535"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HMA05568.1"
                     /translation="MAAETDPFAGRQAPSFCLPDAGGKEVCLENYRGKWVVLYFYPRD
                     NTPGCTLEAIQFSKALEEFASLGAQVLGVSTDSPESHVKFAEKHDLTVLLLSDPDHRA
                     ITAYDAWKPKIFFGKEILGTERDTFLIDPEGKIVEVWRRVSVKGHAEAVKEALLARKA
                     GKA"
     gene            1405..1881
                     /locus_tag="VKO45_06540"
     CDS             1405..1881
                     /locus_tag="VKO45_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HMA05569.1"
                     /translation="MDQPRMIQPGDIAPNFSIKDQNDKTFDLLEQSGKRVLLSFHPLA
                     WTAYCAGQMKSLEENRQALGSLNTVAVGISVDSVPCKKAWAESLKITDTRLLCDFWPH
                     GKVAQMYGLFRDANGFAERANVIVDERQTAVFVKVYPIHSVPDIGEVLGFLETLKR"
     gene            complement(2015..2860)
                     /locus_tag="VKO45_06545"
     CDS             complement(2015..2860)
                     /locus_tag="VKO45_06545"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HMA05570.1"
                     /translation="MSPLQTRQFGKNGPPVTIVGLGGEGVLRSHGRNAQARPVIEEAV
                     SQGITYFDSARAYDESELYLGRFWEAKAGQRSRVFQTSKSARRDYAGAWADLETSLRN
                     LHTSHLDLWQMHDLRNAEDLKTLEGSSGALDAFLEARRQGKARHIGVTGHEDPAILTH
                     AVENWPVDSVLLPVNPIEGVLGGFLDGTLPAALRKGIAVIGMKVLGAGNFLAPTGGIT
                     AELLIRYALSRPVTLIIVGCSLPLEVLTLASLGRDFIPLPEDDQRRLEERFAPYARDL
                     AFYRA"
     gene            complement(2995..3798)
                     /locus_tag="VKO45_06550"
     CDS             complement(2995..3798)
                     /locus_tag="VKO45_06550"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMA05571.1"
                     /translation="MALRDEKFSIPEELIIEDLAGQDHRIRQNALDLLNVMTWYDWDP
                     TKFVASGGGQALISCLEDPDEEARRLAAFLVGYVAEACGVESSGVASAVPLLMKLFDG
                     SSASLSKKILSRGSRNLRVAAGNTLVKIGTPAVEALTVALHNPNKDVRFNAARALGEI
                     RDPRAVESLLDLLRDQNRTVRLAAARALGFLKDVRAVEPLSHTLGDRARMVRNYVAWA
                     LGEIGDERAVPALQKAYKHSLMEGDLSGQFAINAALEEIESSRYRKYRE"
BASE COUNT          742 a         1271 c         1255 g          745 t
ORIGIN      
        1 tgatattcgt catcgtaatc gcatttttaa ctttcctcgg agcattgttt tactccatcg
       61 ggaccggtat tccccggtaa atctttttat ccgttccgag aatgaatata aagtgtcatt
      121 tctccgaatc gggaaatgag aagagtaata gtctaatcat caggcacccc cgtggtgtag
      181 cggtcaatca tgcaggcctt tggagccggc gacggcggtt cgaatccgcc cgggggtatc
      241 tcctcgtttt tacaacccct attcctcgcc gatgtcctcc ttccacagat ccggtcttgc
      301 ggcgatgaaa tcacgcatca tgcggatgca ctccccgtca tccaggatga ccagctccac
      361 cccccgcgag cggaggtact cctccggtcc ccggaaggtc ctgttttcgc cgatcacgat
      421 cttcgggatc ccgtacagga gggctgcccc gctgcacatg tcgcacggag aaagggtcga
      481 gtacaggatc gcccgccggt agtcagaggg ccggaggcgg cccgcatgtt caaggcagtc
      541 catctcggcg tgcagggtgg ggcttccctg ctgcaccctg cggttgtgac cctgccccac
      601 gattcttccg ccgatgacga gcaccgagcc aatggggatg ccgccttctg cgagtcccct
      661 tcttgcctct ccaatggcag cctgcatgaa tccgtccatg aaaccatcca tcccagggat
      721 gggttggatt tccggcatat caatgccggg caagggttcc tggcctcaac agctggtgcc
      781 ggaaccaagc gggaggcaat ccttatgtcc ctccgggatc atcccatcct gatctttatg
      841 gctgccgaaa cagatccctt cgccggaagg caggccccct ctttctgcct ccctgatgcc
      901 gggggtaagg aggtctgcct ggaaaactac cgcgggaagt gggtggtgct ctacttctac
      961 ccccgggaca acactccggg ctgcaccctc gaggccatcc agttctcgaa ggcccttgag
     1021 gagttcgcct ccctcggggc ccaggtcctc ggggtgtcca ccgattcgcc cgagagccac
     1081 gtgaagttcg ccgagaagca cgacctcacg gtgctcctcc tctcggatcc cgatcaccgc
     1141 gccatcactg cctacgatgc ctggaagccg aagatcttct tcgggaagga gatcctcggg
     1201 accgagcggg acaccttcct catcgatccc gagggaaaga tcgtggaggt ctggcgccgg
     1261 gtgagcgtga agggccatgc agaggccgtg aaggaggccc tcctcgcaag gaaagccggg
     1321 aaggcgtgag ccgcgggccg gccggcaccc ggagactgcg aaggctctat ataggatatt
     1381 gcaggaagat caggcagtga tacgatggac cagccgcgga tgatccagcc gggagacatc
     1441 gccccgaact tctccatcaa ggaccagaac gacaagacct tcgacctcct cgagcagtcg
     1501 ggaaagcggg tgctcctctc cttccatccc ctggcctgga ctgcctattg cgcggggcag
     1561 atgaagtccc tcgaggagaa ccggcaggcc ctcgggtccc tgaacaccgt cgcagtcggg
     1621 atcagcgtgg actcggtccc ctgcaagaag gcctgggccg aaagcctgaa gatcacggac
     1681 acccggctcc tctgtgactt ctggccccac gggaaggtcg ctcagatgta cggtctcttc
     1741 cgagacgcga acggctttgc cgagcgggcg aatgtcatcg tcgacgaaag acagaccgcg
     1801 gtcttcgtga aggtctaccc catccacagc gtgccggaca tcggggaagt cctcgggttc
     1861 ctcgagaccc tgaaaaggta acgacccctt tccctcatct ccgatcacct gcctgatcgg
     1921 tcggtctccc gaagagcgag ccaaccttct cggaaaaagc aaaccgcgcg tgccccgggg
     1981 gccgggaagg tttgattgtt cccgatcttt caggtcacgc ccggtaaaag gcgaggtccc
     2041 tcgcgtacgg ggcgaacctt tcctcgagcc ggcgctggtc gtcctcgggg agcgggatga
     2101 aatccctccc cagggaggcg agggtgagga cctcgagcgg tagggaacac ccgacgataa
     2161 tgagggtgac cggccgggag agggcgtagc ggatcaggag ctccgccgtg atcccgcccg
     2221 tgggggcgag gaagttcccc gccccgagga ccttcatccc gatgacggcg attcccttcc
     2281 tgagggccgc aggaagggtc ccgtccagga acccgccgag aaccccctcg atgggattca
     2341 cagggaggag caccgagtcc acgggccagt tttccaccgc gtgggtgagg atcgccggat
     2401 cctcgtggcc cgtgaccccg atatgcctcg ccttcccctg ccgccgtgcc tccaggaagg
     2461 cgtcaagggc acccgaggag ccctcaaggg tcttcaggtc ttccgcattc ctcaggtcgt
     2521 gcatctgcca gaggtccagg tggcttgtgt ggaggttccg gagggaggtc tcgagatcgg
     2581 cccatgcgcc ggcatagtcc cgccgggccg atttcgatgt ctggaagacc cgggacctct
     2641 ggcctgcctt cgcctcccag aacctcccga ggtacagctc gctctcgtca taggcccggg
     2701 ccgagtcgaa gtaggtgatt ccctgggaga ccgcctcctc gatgaccggc cgggcctggg
     2761 cgttcctccc gtggctgcgg agaacgccct ccccgcccag cccgacgatg gtgacggggg
     2821 ggccgttctt cccgaattgc cgggtctgga ggggcgacat attccggcct cacccgggaa
     2881 agttccgtga aggggacatg tcgagccacc accggataag ggaacgggat gggaagcggg
     2941 aaaatagacg accggacgaa gacgatcccg gaaaaagggg tagggccatc gcccctattc
     3001 ccggtacttc cggtacctcg agctctcgat ctcctcgagg gccgcgttga tggcgaactg
     3061 gcccgagagg tccccctcca tgagggagtg cttgtaggcc ttctggaggg cggggaccgc
     3121 cctctcgtcg ccgatctcgc ccagcgccca ggcgacatag ttcctgacca tccgtgccct
     3181 gtcgcccagg gtgtgggaga ggggctccac ggcccggacg tccttgagga acccgagggc
     3241 ccgggcggcc gcgagcctga cggtgcggtt ctggtcgcgc aggaggtcga ggagggactc
     3301 cacggccctc gggtcacgga tctccccgag ggccctcgcc gcgttgaacc gcacgtcctt
     3361 gttcgggttg tggagggcga cggtcaaggc ctcgaccgcg ggggtgccga tcttcacgag
     3421 ggtgtttccc gcggcgaccc ggaggttcct cgagccccgg gagaggatct tcttcgagag
     3481 ggaggcggag gagccgtcga agagcttcat gaggagggga acggcgctcg cgacaccgga
     3541 gctctcgacg ccgcaggcct cggcgacata cccgacgagg aaggccgcga gcctcctggc
     3601 ctcctcgtcc gggtcctcga ggcaggagat gagggcctgg ccccccccgg aggcgacgaa
     3661 cttcgtcggg tcccagtcgt accaggtcat cacgttcagg agatccaggg cgttctggcg
     3721 gatccgatgg tcctgcccgg cgagatcctc gatgatcagt tcctcgggta tggagaactt
     3781 ctcgtccctc agtgccattc caatcctccg cttccttaaa ttgaataaga tataaagagg
     3841 ggtggataaa agcgttccgt aatcagggca gccacccctc gctcaccgag gccgggtgca
     3901 tccggtttct tccctggaga agggcaggtg cagccgggtg cagctgcagg ctctgaacta
     3961 tctaatcgtt gatatttgcc ctgttttaga aacaaccata ttatagcact taa
//
LOCUS       DATGDI010000175         5494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_47344, whole genome shotgun sequence.
ACCESSION   DATGDI010000175 DATGDI010000000
VERSION     DATGDI010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47344"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..2123
                     /locus_tag="VKO45_06555"
     CDS             114..2123
                     /locus_tag="VKO45_06555"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HMA05572.1"
                     /translation="MTETYDASAITVLEGLEPVRKRPAMYIGSTDTRGLHHLVYEAVD
                     NSVDEALAGYCNLIEVIVHRDGSCSVEDNGRGIPVDVMPQYGKSALEIVMTVLHAGGK
                     FDKATYQVSGGLHGVGVHVVNALSQKLSVQVYRNGNRYTMEFERGLMTKGLQRQKETL
                     QDLKGRFSRRYGRIEPPFDSGITDPAAYLAAAPLPLTGTVITFLPDPDIFETVTFDYD
                     VLSHRLRELAFLNRGVTFRIRDERTGDGGETFCYQGGIAEYVTFLVGETAKVHEEVIF
                     LTGKDVDQSVEAEIALQYTKGFSETVFTYVNSVNTREGGTHLEGFRTAITRAINAAAK
                     RANLFKNSDSPLRGEDVREGLTAVISLKMAHPQFEGQTKMRLGNSAVRGIVDSLVYSC
                     LLEFFDEHPKVLATVVEKGLLAARAREAARSARDLARRKSSLESAGLPGKLADCSERE
                     PEKSELFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNVEKATEEKMLKNAEIQTLIS
                     AIGTGIKEEFDPEKSRYHSIVLMTDADVDGAHIRTLLLTFFFNEMRALIERGYIYIAM
                     PPLFRVAKGKQEQYFYREDEMKKALTKLGEKGVSVQRYKGLGEMNAIQLWSTTMDPAQ
                     RTLKKVTIEDAAYAAEIFYKLMGEDVDARRDFIRRHAKEVTNLDI"
     gene            2140..4575
                     /gene="gyrA"
                     /locus_tag="VKO45_06560"
     CDS             2140..4575
                     /gene="gyrA"
                     /locus_tag="VKO45_06560"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HMA05573.1"
                     /translation="MPVKIEDEMRVSYIDYAMSVIIGRAIPDVRDGLKPVHRRTLFAM
                     WEMGNTSDKPYKKSARVVGDVMGKYHPHGDTAIYDTLVKMAQDFAYRTPLVDGQGNFG
                     SIDGDAAAAMRYTEVRLTRAAEELLKDLEKETVPFAPNFDESLKEPLVLPAKIPNLLV
                     NGTNGIAVGMATSMLPHNLGEACRAVTAYLDNPSITVEELLPILPAPDFPTGGIIMGT
                     SGIHEAYQTGRGKCVVRGVAEITDEEKNPSILITEVPFQVNKARLVEEIANLVKEKKI
                     EGISDVRDESDKEGIRVVIELRKGSMPAVVLNQLYKHTPLETSYGIINYAIVDGQPRV
                     LSLLDLLREFVLHRMSMVRKRTQFDLKKIEERVHILKGLLLALASIDAVIATIRAADS
                     AEKAKIDLTARFALDAPQADAILKMQLRTLAGLERKKIQDEHDEGQKEILRLREILSD
                     DTHIRTEIRKEMEEMAGKFGNERRTRISSDVTDITKEDLIENRAMLVSLTGENYIKRM
                     ALETYRKQHRGGRGIMGMAVKEEDVVEDAFVADMLDYLLCFTNLGRVYWLKVYNIPEA
                     ARQAKGKPIVNLLNLEDEKVTNVIPVKGFGESAFLIFGTKKGKVVKIPLAEFSRPRPS
                     GLNAFTLREGDELVDVKRTDGNRELILTTKKGRSLRIHENTIPMRHRNAMGVIGIRTR
                     GTDVLQSLTVLEHDHLLTITDSGYGKRTEFDEFRGHGRGTQGVINIRMDYENGVVAAQ
                     SVSPGDEIIVMSSGGNVMRTRVDEISIQKRNTRGVRIMNLEGGDRIVGVGVVRPETNG
                     ENGSPPPAARPPS"
     gene            4633..5076
                     /locus_tag="VKO45_06565"
     CDS             4633..5076
                     /locus_tag="VKO45_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HMA05574.1"
                     /translation="MYRTVIAVDTRGEGDIVDITGEVEKALRESRVVEGLVNLFVTGS
                     TAALTTIEFEPGVLSDLKRSLAIIAPSNLSYAHDGKWGDGNGRSHVKAAIVGPSLTIP
                     VGKGLLDCGTWQQIVLLELDTRPSRHRTITCTIMGEQGTGKTPKK"
     gene            complement(5129..5392)
                     /locus_tag="VKO45_06570"
     CDS             complement(5129..5392)
                     /locus_tag="VKO45_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HMA05575.1"
                     /translation="MPRPDDTIRACIQMLQQIMEDGTIPRNIRRVADETRAILLDAGK
                     PIGLRAATAISKIDEVSNDPNMPVHARTRIWELVSQLETVPLD"
BASE COUNT         1109 a         1783 c         1763 g          839 t
ORIGIN      
        1 catcctcccg cccgacgtaa aagcactatt ataagccccg gaatccaata ataggtaatg
       61 cagcgttccc gggggaccta cctagatcac cctcttttcc ggggaggagt tgcatgaccg
      121 agacctacga cgcctcggcc atcaccgtcc tcgagggcct cgagcccgtc cggaagcggc
      181 cggcgatgta catcgggtcg actgacaccc gcgggctcca ccacctggtc tacgaggcgg
      241 tggacaactc ggtggacgag gccctcgccg ggtactgcaa cctgatcgag gttatcgtcc
      301 acagggacgg ctcctgctcg gtggaggaca acggccgggg gatcccggtg gacgtgatgc
      361 cccagtacgg gaagtcggcc ctcgagatcg tcatgacggt gctccacgcc ggcgggaagt
      421 tcgacaaggc cacgtaccag gtctcgggcg ggcttcacgg cgtgggcgtg cacgtggtga
      481 acgccctctc gcagaagctc tccgtgcagg tgtaccgcaa cgggaaccgg tacacgatgg
      541 agttcgagcg ggggctcatg acgaaggggc tccagaggca gaaggagacc ctccaggacc
      601 tgaaggggcg tttttcccgc cgctatggcc gcatcgaacc ccccttcgac tccgggatca
      661 ccgatccggc ggcatacctc gccgccgccc cgctccccct caccgggacc gtgatcacct
      721 tcctccccga cccggacatc ttcgagacgg tcaccttcga ctacgacgtc ctctcccacc
      781 ggctccggga gctcgccttc ctcaaccggg gggtcacctt ccggatccgg gatgagcgga
      841 ccggcgacgg cggtgagacc ttctgctacc agggcgggat cgccgagtac gtcaccttcc
      901 tcgtggggga aacggcgaag gtccacgagg aggtcatctt tctcacgggg aaggacgtgg
      961 accagagcgt cgaggccgag atcgccctcc agtacacgaa gggcttctcc gagacggtgt
     1021 tcacctacgt aaacagcgtg aacacccgcg agggcggcac tcacctcgag gggttccgca
     1081 cggccatcac gagggcgatc aacgccgcgg cgaagcgggc gaacctcttc aagaacagcg
     1141 actcccccct ccggggcgag gatgtccggg agggactcac cgcggtgatt tccctgaaga
     1201 tggcccaccc ccagttcgag ggccagacga agatgcgcct cgggaactcg gcggtgcggg
     1261 ggatcgtgga ctccctcgtg tacagctgcc tcctcgagtt cttcgacgag catccgaagg
     1321 tcctcgcgac cgtcgtggaa aaggggctcc tcgccgcgcg ggctcgcgag gcggcccgga
     1381 gcgcccggga cctggcacgg cgcaagtcct ccctcgagtc ggccggcctc ccggggaagc
     1441 tcgccgactg ctcggagcgg gaaccggaga agagcgagct ctttatcgtc gagggggaca
     1501 gcgccggcgg ttcggcgaag cagggccggg accggaggtt ccaggccatc ctccccctcc
     1561 gggggaagat cctgaacgtg gagaaggcca ccgaggagaa aatgctcaag aacgccgaga
     1621 tccagaccct catctcggcc atcggcaccg gcatcaagga ggagttcgac cccgagaagt
     1681 cccggtacca tagcatcgtc ctcatgaccg atgccgacgt ggacggggcc cacatccgga
     1741 ccctcctcct caccttcttc ttcaacgaga tgcgggccct catcgagagg ggctacatct
     1801 acatcgccat gccccccctc ttccgggtgg caaaggggaa gcaggagcag tacttctacc
     1861 gggaggacga gatgaagaaa gccctcacca agctgggaga gaagggggta agcgtccagc
     1921 ggtacaaggg cctcggagag atgaacgcca tccagctctg gtccacgacg atggaccccg
     1981 cccagcggac cctgaagaag gtgaccatcg aggacgccgc ctacgccgcc gagatcttct
     2041 acaagctgat gggggaggac gtggacgccc ggcgtgactt catccgccgg cacgcgaagg
     2101 aggtgacgaa ccttgatatc tgaccgtcct caccgacagg tgccggtcaa gatcgaggac
     2161 gagatgcggg tctcgtacat cgactacgcg atgtccgtga tcatcgggag ggccatcccc
     2221 gacgtgcggg acggcctgaa gcccgtccac cggcgcaccc tcttcgccat gtgggaaatg
     2281 ggcaacacga gcgacaagcc ctacaagaag tccgcccgcg tggtcgggga cgtgatgggc
     2341 aagtaccatc cccacgggga caccgccatc tacgacaccc tcgtgaagat ggcccaggac
     2401 ttcgcctacc gcacacccct cgtcgacggc caggggaact tcgggtccat cgacggggac
     2461 gccgccgcgg ccatgcggta caccgaggtc cggctcaccc gggccgccga ggagctcctg
     2521 aaggacctcg agaaggaaac ggttcccttc gccccgaact tcgacgagtc cctgaaggag
     2581 cccctcgtgc tcccggcgaa gatcccgaac ctcctcgtca acggcacgaa cgggatcgcg
     2641 gtcgggatgg ccacctccat gctcccccac aacctcgggg aggcctgcag ggcggtcacc
     2701 gcctaccttg acaacccctc catcaccgtg gaggaactcc tccccatcct tcccgccccc
     2761 gacttcccca ccggcgggat catcatggga acctcgggga tccacgaggc gtaccagacg
     2821 ggcaggggga agtgcgtggt ccggggagtg gccgagatca ccgacgagga aaagaacccg
     2881 tccatcctca tcaccgaggt ccccttccag gtgaacaagg cccgtctcgt ggaggagatc
     2941 gcaaacctcg tcaaggagaa gaagatcgaa gggatctccg acgttcggga cgagtccgac
     3001 aaggagggga tccgggtggt catcgagctc cggaagggct ccatgccggc cgtggtcttg
     3061 aaccagctct acaagcacac ccccctcgaa acgagctacg ggatcatcaa ctacgccatc
     3121 gtggacgggc agccccgggt cctctccctc ctcgacctcc tccgggagtt cgtcctccac
     3181 cggatgtcca tggtccggaa gcggacccag ttcgacctga agaagatcga ggaacgggtc
     3241 cacatcctga aggggctcct cctcgccctc gcctccatcg acgcggtcat cgcgaccatc
     3301 cgggcggccg actcggccga gaaggccaag atcgacctga cggcgcggtt cgcgctggac
     3361 gccccacagg ccgatgcgat cctgaagatg cagctccgca cccttgcggg gctcgagcgt
     3421 aagaagatcc aggacgagca tgacgaggga cagaaggaga tcctccggct cagggagatc
     3481 ctctcggacg atacccacat ccgcaccgag atccggaagg agatggagga gatggccgga
     3541 aagttcggga acgaacgccg gacccggatc tcgagcgatg tcacggatat caccaaggag
     3601 gacctcatcg agaaccgggc catgctcgtc tccctcaccg gggaaaacta catcaagcgc
     3661 atggccctcg agacctaccg gaagcagcac cggggaggcc gggggatcat gggcatggcc
     3721 gtgaaggagg aggacgtggt cgaggatgcc ttcgtggccg acatgctcga ctaccttctc
     3781 tgcttcacga acctgggccg ggtctactgg ctgaaggtct acaacatccc cgaggcggcc
     3841 cggcaggcga aggggaagcc catcgtgaac ctgctcaacc tcgaggacga gaaggtgacg
     3901 aatgtcatcc cggtgaaggg cttcggggag agcgcgtttc tgatcttcgg cacgaagaag
     3961 gggaaggtcg tgaagatccc cctcgcggag ttctcccggc cgcgaccctc ggggctgaac
     4021 gccttcaccc tccgtgaggg ggacgagctc gtggacgtga agaggacgga cggaaaccgg
     4081 gagctgatcc tcaccacgaa gaagggccgg tccctccgga tccatgagaa caccatcccc
     4141 atgcgccacc ggaacgccat gggggtcatc gggatccgga cccgggggac cgatgtcctc
     4201 cagagcctca cggtcctgga gcatgaccac ctgctcacca tcaccgactc ggggtacggg
     4261 aagcggaccg agttcgatga gttccggggc catggccggg ggacccaggg ggtcatcaac
     4321 atccggatgg actacgagaa cggggtggtc gccgcccagt cggtctcgcc cggcgacgag
     4381 atcatcgtga tgtcctcggg agggaacgtg atgcggaccc gggtggacga gatctccatc
     4441 cagaagcgca atacccgggg cgtccggatc atgaacctgg agggggggga caggatcgtc
     4501 ggggtgggcg tcgtccgccc cgagacgaac ggggagaacg ggagcccccc gcctgccgca
     4561 cggccaccct cctgagacgc aagaaagaac cttcatatag agggaaactc acgtttttcc
     4621 cgggagtacc cgatgtaccg caccgtgatc gccgtcgata cccgggggga gggggacatc
     4681 gtggacatca ccggggaggt cgaaaaggcc ctccgggaga gccgggtcgt ggaggggctc
     4741 gtgaacctct ttgtcaccgg gtccaccgcg gcccttacca ccatcgagtt cgaaccgggg
     4801 gtgctctcgg acctgaagcg ctccctcgcc atcatcgccc cttcaaacct ttcctatgcc
     4861 catgacggga agtggggaga cgggaatggc cggtcccatg tgaaggcggc catcgtcggg
     4921 ccgtccctca ccatcccggt gggaaagggt ctcctcgact gcggcacctg gcagcagatc
     4981 gtcctcctcg agctcgacac ccggccgagc cggcaccgga ccatcacctg caccatcatg
     5041 ggcgagcagg gtacggggaa gacaccaaag aaatgagaca acggggggat cggcccccga
     5101 cccgtctgcg cttcaggaag ggaaattctt aatcgagcgg caccgtttcc agctgcgaga
     5161 cgagctccca gatgcgggtc cgtgcgtgca ccggcatgtt gggatcattg gacacctcgt
     5221 cgatcttgga gatggcggtc gccgcgcgca ggccgatggg tttcccggcg tccaggagga
     5281 tggccctcgt ctcgtctgcc acacgccgga tgttccgggg gatggtcccg tcctccatga
     5341 tctgctggag catctggatg cacgcacgga tggtatcgtc tggccttggc atggtaatcc
     5401 ctttatgatt catcatatca acccatatat accttgataa acggagtgaa ggtcatggga
     5461 gagaagaaac cgcggaaccc cgacgatatc aggg
//
LOCUS       DATGDI010000176         8597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_105518, whole genome shotgun sequence.
ACCESSION   DATGDI010000176 DATGDI010000000
VERSION     DATGDI010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8597
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105518"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VKO45_06575"
     CDS             <1..274
                     /locus_tag="VKO45_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HMA05576.1"
                     /translation="RGISKDRAKIDLWLKDAGASTELRTWYGHDPQRWKEFRRRYFGE
                     IRQRPEVVKLLRDAEREKGTVTFVFAARDEVHNNAVALKEFLEEGT"
     gene            complement(336..1724)
                     /locus_tag="VKO45_06580"
     CDS             complement(336..1724)
                     /locus_tag="VKO45_06580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMA05577.1"
                     /translation="MVSEAGRRQVLLASVLGSSLAPFMVSALVVALPAIADEFSAGAV
                     SLGWLTNVFFLSAAVFLLPMGRIADRHGVKKVFVAGIGVYLVSSVLSLLSPSLPFLIG
                     ARFLTGIGGGMIFGTSIALLSLSFPESARGKAIGMNVTGMFAGFLLGFLLGGLLTFYV
                     GWRSIFLVTIPVELVVMGLVFLRIRGECELSRSRDLDLPGLVLYSLSILLLMVGFSAL
                     PGVSGGILLAGGMLALCLFILRELRAESPALDVRLFTRNRTFGRANLAALLFNTSNFA
                     VIFLLSLYLQDIRGFDARYSGAILLTTVIFMALFSPYAGRLSDRRAPGVLVGSGVILS
                     SIGLLILTLLDGSTPVTLIILALAVMGAGFAFFFSPLLRTLVGSVPREMYGRASGMVE
                     SMRLIGMTVSIALTTIALSATIGSVHVTPGISGAFIGTIRLVLWIFLAFSLAALAVSL
                     SLKREVADQDGP"
     gene            1870..3300
                     /locus_tag="VKO45_06585"
     CDS             1870..3300
                     /locus_tag="VKO45_06585"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMA05578.1"
                     /translation="MLMRIGGKEVGSAKGTWLDVINPATGEVLDRVPAGGEEEIGMAV
                     EAGEHAFEAWAGKTPRERGKIFFHAAGEVRAVHQDLARTLTREQGKPLKQAVDEVRGF
                     ANILEFYASISGCLAGDTGRLGPHQDYATVREPYGVCGAIIPWNVPAIIMGWKVSASL
                     LAGNTLVLKPASSTPLTNLALAGILERSGLPPGVLNVVTGTGDTAGQALVMHPGIHRI
                     SFTGQIETGKAILAKTAPLLKEITLELGGSDPMIVWKDADLPKAVEGVVHSRFYNAGQ
                     TCTAVKRLFVHEEIAPRFLEALLARIRSLRVGDGLSSGTDMGPLQNRAQRERIEGQVN
                     RLREREEGHILTGGERPTGTGYDRGFFYAPTLVTDVDPGAAILREETFGPVLPFVTVP
                     DLESAIREANRTRYGLGASVWTRDIGVSREVTGRVRAGMVWINRHMPVPPEVPFGGTK
                     ESGIGRENGLQALAQWTRTKSIVTVW"
     gene            complement(3328..4725)
                     /locus_tag="VKO45_06590"
     CDS             complement(3328..4725)
                     /locus_tag="VKO45_06590"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HMA05579.1"
                     /translation="MRQEKDSLGMRDVPEEVYYGIQTLRAVENFPVTGRREPPELVKA
                     YALLKQAAALANMDLGVLDRPRGEAIASAAAEVASGKLADQFPVDIFQAGAGTSFNMN
                     VNEVIANRALESLGRRKGEYEFLSPNDHVNLSQSSNDTFPTASHLAVIGEADRLLLRL
                     RELVQALERKGREFQTLPKTGRTHLMDALPVTLGDEFQAYATALARGSVRIRERRDAL
                     RELAIGGTATGTGAGAPPGFRDAVVGHLSRLTGLPLFSALDSLEALQSRSQMAAFSGA
                     LRELALELIRIANDLRIMGSGPTSGLDEIHLPPVQPGSSIMPGKVNPVMAECLDMVAF
                     QVIGNDTALSLAAQAGQFELNVMTPLMTHTLLDSLGMLNRYLPAFTERCIDGIQANEV
                     RLVAYIRLNPSLATLLTPRIGYLRAARLAEESMEKKRSVRDLAIEKGILTEKEADALF
                     DLEDQSRNRYRDERM"
     gene            complement(4722..5504)
                     /locus_tag="VKO45_06595"
     CDS             complement(4722..5504)
                     /locus_tag="VKO45_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HMA05580.1"
                     /translation="MLKVYRKGPGAAPPRYDSFDMESEPGMTVLSALFRAQEMDETLA
                     FRYSCRGAVCGTCAMLINKVPGLACRTQVRDLLEGKGRMTLAPYPALEVTVPWDPARE
                     VLVEPLPHLPVIRDLIVDMSLFFEYYRFIGPVLSPGTPAPGRERPMSPAAVKEVATYA
                     NCILCGACFGACPVDGKNPRYLGPAALAALYRFRIDPREGDGASRLRRADTPDGWWAC
                     EFHANCRRVCPKGVPPNLAIGRARQELMAAGGRSAGPEERKP"
     gene            complement(5507..7279)
                     /locus_tag="VKO45_06600"
     CDS             complement(5507..7279)
                     /locus_tag="VKO45_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMA05581.1"
                     /translation="MYTDSAISAWDAVVIGGGLTGLRAALRLSRAGLAVAVISKVHPL
                     RSHSVAAQGGMNAALGNVPGEDGTADSWENHAFDTVKGSDYLADQDAVALMCREAPAT
                     VVELEHMGTVWSRLPDGRIAQRPFGGAGFPRTCYAEDRTGHNVLHTLYQQVLGQGIPV
                     FEEHFVTSLVTDAGRCTGCTALEIMTGAVRGFAARAVLLATGGFGRVFSRSTNALINT
                     GDGMALALRAGAPLKDMEFVQFHPTSLHGTNILISEAARGEGGFLLNRHGERFMERYA
                     PRALELAPRDVVARAIAEEIERGNGFGGGYVHLDLRHLGPAIIGERLPGIRQIALDFA
                     GVDPVEQPIPVQPGQHYSMGGVDVDIAGRSGLPGLYAAGECACVSVHGANRLGGNSLL
                     ETVVFGRLVAGSMESDLKDLPEPRREPIESAVRATGELIREILGRTGGEPGGRKPEGE
                     TTGRSPGVDSPFPLIREVQETMFGHFGIFREGPPMEEGLRRIRGIRERASLASPGNRE
                     AAVNQALVRYLELEMMLLLAETVARGAIAREESRGSHFRRDFPKRDDERFLSHTIAGM
                     REGEVSIGYKPVTLGMFGVEERGY"
     gene            7383..>8597
                     /locus_tag="VKO45_06605"
     CDS             7383..>8597
                     /locus_tag="VKO45_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HMA05582.1"
                     /translation="MPVEEGEVSVLIGGKAGEGISSAGQVIAHLLGQLGYRVHMHFDY
                     PSLIKGGHNFAIVRGSGRRIGAVGNRVDLLLALDQETLRLHLPRLPAGQESGAVVYNR
                     DTAKYPPGVGIPVAAILAAEKAPPVMGNSAILGAFVRAAGIGWPVAIGVLGKSFRKAT
                     EQNLRVARRGYEASEERLRIPSLGLPPLPAFTGNEAMGIGLVEAGLGVYLSYPMSPTS
                     NLMHFLAAHADETRIRVLQPESEIAVILASLGCAYAGTRAAVGTSGGGFSLMVEALGL
                     AGMAELPVVIVLGQRAGPSTGLATYTAQADLEFAIHAGQGGFPRLVIAPGDAEEARTW
                     IRAAVDLAWKYQLPALFLPDRIVCEGMYSLDPGAAPEYQAFPAPADPGTHPYLRYAIT
                     DSGISPLRFPPIK"
BASE COUNT         1452 a         2897 c         2969 g         1279 t
ORIGIN      
        1 ccggggcatc tcgaaggatc gcgcgaagat cgacctctgg ctgaaggatg cgggggcgag
       61 cacggaactt cgcacctggt acgggcatga cccgcagaga tggaaggaat tccggcggag
      121 gtacttcggg gagatccggc aaaggccgga ggttgtgaaa ctcctcaggg atgccgagag
      181 agagaagggc accgtgacct tcgtctttgc ggcccgggat gaggtgcaca acaacgccgt
      241 ggcgctgaag gagttcctgg aagaggggac ctgagggcgt tttttccccg gatttccctt
      301 acgaagtatc cggacaggcc tcccgtaagg cctgcctacg gaccatcctg atccgcgacc
      361 tccctcttca gggagaggga gacggcaagg gcggcaagag aaaaggcgag gaatatccag
      421 agcacaaggc ggatggtccc gatgaaggca ccggagatcc ccggggtgac gtgcacgctc
      481 ccgatggttg cggaaagcgc gatggtggtg agggcgatgc tcacggtcat cccgatgagc
      541 ctcatggact cgaccatgcc cgaggcccgg ccgtacatct ccctggggac cgagcctacc
      601 agggtccgga ggaggggtga aaagaagaag gcgaaaccgg cccccatcac ggcgagggcg
      661 aggatgatga gggtaacggg ggtagagccg tcgaggagag tgaggatgag gaggccgata
      721 gaggagagga tcaccccgga tcccaccagt accccggggg ccctgcggtc cgacaatctc
      781 cccgcgtacg gggagaagag ggccatgaag atcaccgtgg tgaggaggat ggcccccgag
      841 taccgggcat cgaacccccg gatgtcctgc aggtagaggg agaggaggaa gatcaccgcg
      901 aagttcgagg tgttgaagag gagggccgcg aggttggccc ttccgaaggt ccggttccgg
      961 gtgaagaggc ggacgtcgag ggccgggctc tctgccctta gttcccggag gatgaagaga
     1021 cagagagcga gcatccctcc cgcgaggagg atccccccgg agacccctgg cagtgccgag
     1081 aatccgacca tgaggaggag gatggagagg gaatagagga cgaggcccgg gaggtcgaga
     1141 tcccggctcc gggagagctc gcactcaccc cggatccgga ggaagacgag ccccatcacg
     1201 acgagctcga cggggatggt cacgaggaag atactcctcc agccgacgta gaaggtgagg
     1261 aggcccccca ggaggaaccc gaggagaaag cccgcgaaca tgccggtgac gttcatgccg
     1321 atggccttcc cgcgcgccga ctcggggaag gagagggaga ggagggcgat ggaggtgccg
     1381 aagatcatgc ctccgccgat gcccgtgagg aacctcgccc ctatcaggaa cgggagcgag
     1441 ggggagagga gagagaggac ggaggagacg aggtacaccc cgatgcccgc cacgaagacc
     1501 ttttttaccc cgtgccggtc ggcgattctc cccatcggca ggaggaagac cgccgccgag
     1561 aggaaaaaga cgttcgtgag ccagccaagg gagacggcgc cggccgagaa ttcatccgcg
     1621 atggccggga gggcgaccac gagggcagag accatgaagg gggcgaggga cgacccgagg
     1681 accgatgcga ggagcacctg cctcctcccc gcctccgaga ccatggggag agaaacgagc
     1741 tcatcggtgg taatcctttc gatccagggc cgccatcctc cgtcgcaccc ctcctgatgg
     1801 cgggttgctc ccgctcggtt ccctgcggga acctcgcacc ttaaatatcc caccgcccac
     1861 ttctcctcca tgctcatgcg catcggaggg aaggaggtcg ggtccgcgaa gggaacctgg
     1921 ctcgacgtga tcaacccggc gaccggggag gtcctggacc gggtcccggc cggcggtgag
     1981 gaggagatcg ggatggcggt ggaggcgggg gagcatgcct tcgaggcctg ggcgggaaag
     2041 accccccggg agcggggcaa gatcttcttc cacgccgccg gcgaggtgcg ggccgtccac
     2101 caggatctcg cccgcaccct gacccgggag caggggaagc ccctgaagca ggccgtggac
     2161 gaggtgcggg gctttgcgaa catcctcgag ttttatgcct ccatctcggg gtgcctcgcc
     2221 ggggacaccg ggcgcctggg cccgcaccag gactatgcga cggtacggga accctacggg
     2281 gtctgcgggg ccatcatccc ctggaacgtg ccggccatca tcatgggctg gaaggtctcg
     2341 gcctcgctcc tcgccgggaa caccctcgtg ctcaagcctg cctcctcgac ccccctcacg
     2401 aacctcgccc tcgccgggat cctcgagaga tccggcctcc cccccggggt gctgaacgtg
     2461 gtgacgggca cgggggacac cgccgggcag gccctcgtca tgcaccccgg gatccaccgg
     2521 atctccttca cgggccagat cgagaccggg aaggccatcc tcgcgaagac ggcacccctc
     2581 ctgaaggaga tcaccctcga actgggcggt tcggacccca tgatcgtctg gaaggacgcc
     2641 gatctcccga aggccgtcga aggggtggtc cactcccggt tctacaacgc cggccagacc
     2701 tgcacggcgg tgaagcgcct cttcgtgcac gaggagatcg ccccccggtt cctcgaggct
     2761 ctcctcgccc ggatccggtc cctccgggtc ggggacggcc tctcttccgg gacagacatg
     2821 gggccgctcc agaaccgggc ccagcgggaa cggatcgagg ggcaggtgaa ccggctccgg
     2881 gaacgggagg aggggcacat cctcaccggc ggggaaaggc caacggggac cgggtacgac
     2941 aggggtttct tctatgcccc caccctcgtg accgacgtgg accccggtgc ggcgatcctc
     3001 agggaggaga ccttcggccc ggtgctcccc ttcgtgaccg tgcccgacct cgagagtgcg
     3061 atccgcgagg cgaaccgcac gcgctacggc ctcggggcat cggtctggac ccgggacatc
     3121 ggcgtgtcac gggaggtcac cggacgcgtc cgggcgggga tggtctggat caaccggcac
     3181 atgccggttc cccccgaggt ccccttcggg gggacaaaag agagcggcat cggcagggag
     3241 aacggtctcc aggccctcgc ccagtggacc cggacgaaga gcatcgtcac cgtctggtaa
     3301 ggggaggggg acccctgccc ccctccttca catcctctcg tcccggtaac ggttcctcga
     3361 ctgatcctcc aggtcgaaga gggcatccgc ttccttctcg gtgaggatcc ccttctcgat
     3421 ggcgagatcc cggacggacc gcttcttctc catggactcc tccgcgagcc tcgccgcccg
     3481 gaggtacccg atccgcgggg tgaggagggt ggcaagggac gggttcaagc ggatatacgc
     3541 cacgagcctg acctcgttcg cctggatccc gtcgatgcac cgctcggtaa aggcggggag
     3601 gtacctgttg agcatcccca gggaatcaag gagggtatgg gtcatgagag gagtcatcac
     3661 gttcagctcg aactgcccgg cctgggcggc cagggagagt gcggtatcgt tcccgatgac
     3721 ctggaaggcg accatgtcca ggcactcggc catcaccggg ttcaccttgc ccggcatgat
     3781 ggaggagccg ggctgcacgg gggggaggtg gatctcgtcg aggcccgagg tcggtcccga
     3841 ccccatgatc cggaggtcgt tcgcgatccg gatcagctcg agggcgagct cccggagcgc
     3901 cccggagaag gccgccatct gggaccggct ctgcagcgcc tccaggctgt cgagagccga
     3961 gaagaggggg agcccggtga gccgggagag gtgcccgacg accgcgtccc ggaagccggg
     4021 cggggcgccg gcccccgtcc ccgtggcggt gcccccgatg gcgagctccc ggagggcatc
     4081 cctccgctcc cggatccgga ccgagcccct cgcgagggcg gtggcatagg cctggaactc
     4141 gtccccgagg gtcaccggga gggcgtccat gagatgggtc cggcccgtct tcgggagggt
     4201 ctggaactcc cttcccttcc tctcgagggc ctggacgagc tcccgcaggc gcaggaggag
     4261 gcgatcggcc tccccgatga cggcgaggtg cgaagccgtc gggaaggtgt cgttactgga
     4321 ctgggagagg ttcacgtggt cgttcgggga gaggaactcg tactccccct tcctccgccc
     4381 gaggctctcg agggcgcggt tcgcgatgac ctcgttcacg ttcatgttga aggaggtccc
     4441 cgcccccgcc tggaagatgt ccacggggaa ctggtcggcg agtttccccg aggcgacctc
     4501 ggccgcggca gaggcgatgg cctccccccg gggcctgtcc aggacgccga gatccatgtt
     4561 ggcgagcgcc gcggcctgct tcaggagggc ataggccttc acgagttccg ggggctcacg
     4621 cctcccggta accgggaagt tctccaccgc acgcagcgtc tggatcccgt agtagacctc
     4681 ctccggtaca tccctcatcc ccagcgaatc cttctcctgt ctcatggttt cctctcctcc
     4741 ggcccggccg aacggccgcc cgccgccatc agctcctgcc ttgcccgccc gatggcgagg
     4801 ttgggcggga cccccttcgg gcagacccgg cgacagttgg catggaactc gcacgcccac
     4861 cagccgtcgg gggtatcagc ccgccggagc cgggaggccc cgtccccttc ccgggggtcg
     4921 atgcggaacc ggtagagggc ggcgagggcc gccggcccga ggtaccgggg gtttttcccg
     4981 tcgacggggc aggccccgaa gcatgcaccg cagaggatgc agtttgcgta ggtggcgacc
     5041 tccttcaccg ccgcggggct catgggccgt tcccgccccg gggccggggt accgggcgag
     5101 aggaccggcc cgatgaaccg gtagtattca aagaagaggg acatgtcgac gatgagatcc
     5161 cggatgaccg gcaggtgcgg gagcggctcg acgagcacct cccgggcagg gtcccagggc
     5221 acggtcacct cgagggcggg gtagggggcg agggtcatcc ggccctttcc ctcaaggagg
     5281 tcccggacct gggtccggca ggcgagccct ggcaccttgt tgatgagcat cgcgcaggtg
     5341 ccgcagaccg ccccccggca ggagtaacgg aaggccaggg tttcatccat ctcctgggcc
     5401 ctgaagaggg cggagaggac ggtcatcccc ggctcggatt ccatgtcgaa gctgtcgtac
     5461 cttggcgggg ccgccccggg ccccttccga tagaccttca gcatcatcag taccctcgct
     5521 cctccacccc gaacattccc agggtgaccg gtttgtaccc gatggatacc tccccctccc
     5581 gcatcccggc gatggtgtgg gagaggaacc gttcgtcatc gcgcttcggg aaatccctcc
     5641 ggaaatggga tccccggctc tcctcgcggg cgatggcccc ccgggccacc gtctccgcga
     5701 ggagaagcat catctccagc tccaggtacc tgacaagggc ctggttcacc gccgcctccc
     5761 ggttcccggg agaggcaagg ctggcccgct cccggatgcc ccggatcctc cggagcccct
     5821 cctccatcgg gggaccttcc cggaagatcc cgaagtgccc gaacatggtc tcctggacct
     5881 cccggatgag ggggaagggc gaatcaacac cggggcttcg ccccgttgtt tctccctcgg
     5941 gcttccgccc tccaggttca ccaccggtcc ggccgaggat ctcccggatc agttcacccg
     6001 tcgcccggac cgccgactcg atcggctctc tccgtggctc cggaaggtcc ttcaggtcac
     6061 tctccatgga gccggcgacg agccggccaa agaccaccgt ctcgaggagt gagttccccc
     6121 cgagccggtt cgcgccatgg acgctcacgc aggcgcactc gccggcggcg taaaggccgg
     6181 gaagaccgct cctcccggcg atgtccacat ccacccctcc catggagtag tgctgcccgg
     6241 gctgcacggg aatcggctgt tccaccgggt ccaccccggc aaaatccagg gcgatctgcc
     6301 ggatccccgg cagccgctcg ccgatgatgg cgggccccag gtggcgcagg tccaggtgga
     6361 catacccgcc cccgaatccg ttcccccgct cgatctcctc ggcgatggcc cgggcgacca
     6421 cgtccctggg ggcgagctcc agggcccggg gggcgtacct ttccatgaac cgctcgccgt
     6481 gccggttcag gaggaacccg ccctcccccc gggcggcctc cgagatcagg atgttcgtgc
     6541 cgtgcaggga tgtcgggtgg aactggacaa actccatgtc cttgaggggg gcccccgccc
     6601 ggagcgcgag ggccatcccg tcgccggtgt tgatcagggc gttcgtggac cgggagaaga
     6661 cccggccgaa acccccggtg gcgaggagga ccgcgcgggc ggcgaatccc ctgacggccc
     6721 ccgtcatgat ctcgagggcc gtgcagccgg tgcaccggcc ggcgtccgta acgagggagg
     6781 tgacgaagtg ttcctcgaag accggtatcc cctgccccag gacctgctgg tacagcgtgt
     6841 ggagcacgtt gtggccggtc cgatcctcgg cgtagcaggt ccggggaaag cccgccccgc
     6901 cgaagggccg ctgggcgatc ctcccgtcgg gaagccggga ccagaccgtc cccatgtgct
     6961 cgagctcgac caccgtggcc ggcgcctccc ggcacatgag ggcgaccgcg tcctggtcgg
     7021 cgaggtagtc cgagcccttc accgtgtcga aggcatggtt ctcccagctg tccgccgtgc
     7081 cgtcctcccc ggggacgttc ccgagggcgg cgttcatccc gccctgggcc gcgacggagt
     7141 gggaccggag gggatggacc ttggagatga ccgccacggc gaggcccgcc cgggagagcc
     7201 ggagggccgc ccggaggccg gtcagccctc ccccgatgac gaccgcgtcc caggccgaga
     7261 tggcggaatc cgtgtacacc ctcctccctt ctggaccggt atttccgccc atcgcttaaa
     7321 acagctccgg tggaggcagg gcaataccct tttattccac tttttccaga tccaaacgga
     7381 ggatgccggt ggaagaaggg gaagtatcgg tcctcatcgg cgggaaggcc ggcgagggga
     7441 tcagcagcgc gggccaggtc atcgcccatc tcctcgggca gctcgggtac cgcgtccaca
     7501 tgcacttcga ttacccctcc ctgatcaagg ggggccacaa cttcgccatc gtgcggggat
     7561 cggggaggag gatcggtgcc gtggggaacc gtgtcgacct cctcctcgcc ctcgaccagg
     7621 agaccctgag gctccacctc ccgaggctcc ccgccgggca ggagagcggt gccgtggtct
     7681 acaaccgcga caccgcgaag taccctcccg gggtcgggat ccccgtcgcg gccatcctgg
     7741 ccgcggagaa ggcgcccccc gtgatgggga actccgccat cctcggtgct ttcgtccggg
     7801 ccgcggggat cgggtggccc gtcgctatcg gggtgctcgg gaaatccttc cggaaggcca
     7861 ccgaacagaa cctccgggtc gcccggaggg gctacgaggc ctcggaggaa aggctccgca
     7921 tcccctctct cgggctgccc cccctcccgg cgtttacggg aaacgaggcc atgggcatcg
     7981 gcctcgtcga ggccggcctc ggggtgtacc tctcgtaccc catgtccccc acctcgaacc
     8041 tcatgcattt cctggccgcc catgccgatg aaacccggat acgggtgctc cagcccgaga
     8101 gcgagatcgc ggtcatcctc gcgtccctcg ggtgcgccta tgccgggacc cgggccgcgg
     8161 tggggacctc cgggggtggc ttctccctga tggtcgaggc cttggggctc gccgggatgg
     8221 ccgagctccc ggtcgtcatc gtcctcgggc agcgggcggg gccgagcacc ggccttgcga
     8281 cctacaccgc acaggcggac ctggagttcg ccatccatgc cggccagggc ggcttcccga
     8341 ggctcgtcat cgccccgggc gacgcggagg aggcgaggac ctggatccgg gccgccgtgg
     8401 acctcgcctg gaagtaccag ctgcccgccc tgttcctccc cgaccgaatc gtctgcgagg
     8461 gcatgtacag tctcgatccc ggggcggccc cggaatacca ggcgttcccg gctcccgccg
     8521 acccggggac ccatccctac ctccggtacg ccatcaccga ctcggggata tcgcccctcc
     8581 ggttcccgcc aataaag
//
LOCUS       DATGDI010000177         1703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_338140, whole genome shotgun sequence.
ACCESSION   DATGDI010000177 DATGDI010000000
VERSION     DATGDI010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338140"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VKO45_06610"
     CDS             <1..333
                     /locus_tag="VKO45_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05583.1"
                     /translation="ADFYSDDAFYLDPAVPGGIQGKDPLLAYFRKLLAQNPNWVWTQI
                     EAIPMEGGFLNKWLARIPIGEKIIESIGVCFVQFDDRGKIRRNEVYFDRTQMMLEMSR
                     LKQVKKVG"
     gene            370..>1703
                     /locus_tag="VKO45_06615"
     CDS             370..>1703
                     /locus_tag="VKO45_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMA05584.1"
                     /translation="MEDSLIEFTEPFERALRLMEETRRHVFVTGRAGTGKSTLLQYFK
                     ANTARQVAVLAPTGVAALNVGGQTIHSFFRFKPDVTPESARKRIRGEGAGIYRRLDAI
                     VIDEVSMVRADLLDCVDAFLRLHGPEAGAPFGGLQMIFIGDLYQLPPVVSAAEKMLFK
                     TLYPSPYFFSALAFRGLEMEFVELEKIYRQQDGRFIDLLNAIRNRTVTEEDLELLNSR
                     LDPDFEPPADEYYIHLTSTNALADGYNEEQLKKLKGRTWTSTGILEGDFGREYLPTAV
                     ALKLKKGAQVMMLNNDAGGRWVNGTIGRVTGFGKDPEGGPLVRVRLNDGRLVEAGPNT
                     WEIFRFFLKDGRLDSEAVGMFTQFPLRLAFAVTIHKSQGKTFERVILDVGRGTFAPGQ
                     LYVALSRCTSLEGIVLRKALRRQHILLDWAVVRYLTRSQYDQASKALSREEK"
BASE COUNT          336 a          535 c          537 g          295 t
ORIGIN      
        1 gccgatttct attccgatga cgcattctat ctcgaccccg ccgtgccggg agggatccag
       61 ggaaaggacc ctctgcttgc ctacttccgg aagcttctgg cccagaaccc gaactgggtc
      121 tggacccaga tcgaggcgat accgatggaa ggcgggtttc tgaacaagtg gctcgcccgg
      181 atacccatcg gggagaagat catcgagagc atcggggtat gtttcgtgca gttcgacgac
      241 cggggaaaga tcaggcgcaa cgaggtctac tttgaccgga cccagatgat gctggagatg
      301 tccagattaa aacaggtcaa gaaggttggc tgacgcgtcc atcggacagg gcgggatgac
      361 ggacctttga tggaagatag tttgatcgag ttcacggaac ccttcgagcg ggccctccgg
      421 ctcatggagg aaacgcgtcg gcacgtcttc gtcacgggcc gcgccggaac cggcaagtcc
      481 accctgctcc agtacttcaa ggcaaacacc gccaggcagg tggcggtcct tgcccccacg
      541 ggcgttgcgg ccctgaatgt ggggggacag acaatccatt cctttttccg gttcaagccc
      601 gatgtgacac ccgaatcggc tcgcaagcgc atccgcggcg agggcgccgg gatctaccgc
      661 aggctcgacg ccatcgtcat cgacgaggtc tccatggtcc gggcggacct gctggactgc
      721 gtggacgcct tcctgcggct ccatggcccc gaggccggcg cccccttcgg cggcctgcag
      781 atgatcttca tcggcgatct ctaccagctt ccccccgtgg tgagcgcggc cgagaagatg
      841 ctcttcaaaa ccctctaccc gagcccctat ttcttcagcg ccctggcctt ccggggcctg
      901 gagatggagt ttgtggagct ggagaagatc taccgccagc aggacggccg cttcatcgac
      961 ctgctcaacg ccatccgcaa ccgcacggtc accgaggagg acctggagct tctcaactcg
     1021 cgcctcgatc cggacttcga gccccccgcc gacgaatact acatccacct caccagcacg
     1081 aacgccctgg ccgacggcta caacgaggag cagctcaaga agctcaaggg caggacgtgg
     1141 acctcgacgg gaatcctgga gggcgacttc ggcagggagt atctcccgac ggcggtggcg
     1201 cttaaactca agaagggcgc ccaggtcatg atgctgaaca acgatgcggg cggccgctgg
     1261 gtgaacggga cgatcggcag ggtcaccggg ttcgggaaag acccggaagg cggccccctg
     1321 gtgcgcgtcc gcctgaacga cgggcggctg gtggaggccg ggcccaacac ctgggaaatc
     1381 ttccggttct tcctgaagga cgggagactg gattcagagg cggtgggcat gttcacccag
     1441 ttccccctga gacttgcctt cgccgtgacg atccacaaga gccagggcaa gaccttcgag
     1501 cgggtgatcc tggatgtggg caggggtacc ttcgcccccg gacagctcta cgtggccctc
     1561 tcccggtgca caagcctcga gggcatcgtg ctgcgcaagg ccctgcgcag gcaacacatc
     1621 ctgctggact gggcggtggt tcgttacctg acccgaagcc agtacgatca ggcctcgaag
     1681 gccctttccc gcgaagaaaa gat
//
LOCUS       DATGDI010000178         3035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_425645, whole genome shotgun sequence.
ACCESSION   DATGDI010000178 DATGDI010000000
VERSION     DATGDI010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425645"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..998)
                     /locus_tag="VKO45_06620"
     CDS             complement(114..998)
                     /locus_tag="VKO45_06620"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMA05585.1"
                     /translation="MDIFTHAAAILVILFASGNAVLIPFGVLGAVIIDIDMAWFLIPR
                     RNPSLYILHHAGCSHSFLGATVLSVISFVTTLALSSAGWLRGTIPGGILVPALVAMLS
                     GAYLHLFLDWLAAPGLPLLYPLTEKRFGLAMTPMPLYFLITVLSLGILGVTLFPGLTM
                     ELAEVYGATFAGIILVSGGMKWLVHRKAPGRSYYTFHPFQWIVIREENPSYTVVVYDM
                     FRGVIRELDFEKYRNIGAEEARRYDGPELRRHRYFSYISTVEKKGSEIIFHDPVREEG
                     LISYPPWYPSVTVSTTAA"
     gene            complement(1047..1625)
                     /locus_tag="VKO45_06625"
     CDS             complement(1047..1625)
                     /locus_tag="VKO45_06625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMA05586.1"
                     /translation="MDFFNSAYRGTPPWDIGRPQKEFVELVRRGEITGSVLDIGCGTG
                     EHALFFAAEGHEVWGIDSAPLAIEKAKEKAEERGLHVHFVLMDVLELRKLNRTFDTAT
                     DSGLFHTLSDEDRPVFVNALTSVLLTGGRYFMLCFSDEEPPGYGPRRITRREIRESFR
                     DGWTIDYIRPATFESRTRTEGPRAWLSSISRK"
     gene            complement(1636..2268)
                     /locus_tag="VKO45_06630"
     CDS             complement(1636..2268)
                     /locus_tag="VKO45_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HMA05587.1"
                     /translation="MTEPVDAIRAIHNAFRNDLRQIDAAALESARGKEGLAATIERFR
                     FMNEVLVWHAHGEEQAIFPAVEQVAPLVAEAYVKDHRGLDMAFEELNRSYAARDPIMT
                     ARATAAFRFHLDIHLDKEDTHLYRIFRERISMPEQGKAVTIMSGNVPRERFPELVAWE
                     FPLIGHDDRENMTRIFQTVMPAPFFATVKEQIKKAIGNDWAELQRRIPTL"
     gene            complement(2324..2959)
                     /locus_tag="VKO45_06635"
     CDS             complement(2324..2959)
                     /locus_tag="VKO45_06635"
                     /inference="COORDINATES: protein motif:HMM:NF019314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HMA05588.1"
                     /translation="MAVGEGSQSILGGYLALFASTEIFIFVFMEIVVKLDAIQRVIFK
                     EAKKADSVGFTLLFGLFSIFGTYIGTTESTGAIVNIRDLAPMVAGLVAGPYAGLADGL
                     IGGIHRFFLGGVTAVPCSLATVLAGVLGGAVYVLNKRRLLGVVPAMFFAGGIEVLHGG
                     LALLLVQPFSLAVEIVVTAIPQMIIANSLGVGIGIIIVHDLIHHYGDAHST"
BASE COUNT          639 a          868 c          932 g          596 t
ORIGIN      
        1 atgggatgca ggatatgagg atggcaagga tggaacatgc caccagaccc tgtttctgca
       61 tatgtaccag tggtccggga atttcaaaag gatattgtcg gtcctgcaaa tgctcaggcc
      121 gcggtagtgc tcaccgtgac agacgggtac cagggcgggt aggagatgag cccctcttcc
      181 cggaccggat cgtggaagat gatctcgctg cccttctttt ccacggtgga gatgtaggag
      241 aaataccggt ggcgcctgag ttcggggccg tcataccggc gagcctcttc ggctccgatg
      301 ttccggtatt tctcgaaatc cagttcccgg atcactcccc ggaacatgtc gtacacaacg
      361 acggtgtagg aggggttctc ttcccggatg acaatccatt ggaaggggtg gaacgtgtaa
      421 taggatcttc cgggcgcctt ccggtgaacg agccatttca tccctccgct gacgaggatg
      481 atgcccgcga acgtcgcccc gtagacctcc gccagttcca tcgtgagacc agggaagagg
      541 gtgaccccga ggatgccgag ggaaaggacc gtgatcagga agtagagggg catgggggtc
      601 atggcgaggc cgaaccgttt ctccgtgagg ggatacagga ggggcaagcc gggagccgcc
      661 agccagtcca ggaacaggtg gaggtaagcc ccggagagca ttgcaacgag agcgggaaca
      721 aggatcccgc ccgggatggt cccccggagc catccagccg aggagagggc gagggtcgtc
      781 acgaaggata ttaccgaaag gaccgtcgcc ccgaggaaac tgtgggaaca cccggcatgg
      841 tgaagaatgt agagggaggg gttccggcgg gggatgagga accaggccat gtcgatatcg
      901 atgatcaccg ccccgaggac gccaaagggg atgagcaccg cgttcccgga ggcaaagagg
      961 atcacgagga tggcggccgc gtgggtgaag atatccatcg gatcccggtg gcatgtaccg
     1021 ggggaaaatg atatggatcc ggggtttcat ttccttgata ttgacgagag ccaggcccgg
     1081 gggccctcgg tcctggtccg gctctcgaag gtcgccggcc tgatgtaatc gatggtccag
     1141 ccgtcccgga agctctcccg tatctccctc cgggttatcc tcctcgggcc gtacccgggg
     1201 ggctcctcgt cgctgaagca gagcatgaag tatcttccac cggttaagag gacggatgta
     1261 agagcattga cgaacaccgg gcggtcctcg tcggacaggg tgtggaagag ccccgaatcg
     1321 gtggcagtat cgaaagtacg gttcagttta cggagttcaa ggacgtccat gaggacaaaa
     1381 tgcacatgga gccccctttc ctctgccttc tcctttgcct tctcgatggc caggggtgca
     1441 gagtcgatcc cccacacctc gtgcccttcc gccgcaaaga agagggcatg ctcccctgtc
     1501 ccgcacccga tgtccaggac agatccggtg atctcccctc tccggaccag ttcgacgaac
     1561 tccttctggg gccggccgat gtcccaggga ggggtgcccc ggtatgcgga gttgaagaag
     1621 tccatacttt tcggcctaca acgtcggtat cctgcgctgg agttcggccc agtcgttccc
     1681 gatggccttt ttgatctgtt cctttactgt ggcgaagaaa ggggcaggca tcaccgtctg
     1741 gaagattcgg gtcatgttct cgcggtcatc atgtcctatg agagggaatt cccatgccac
     1801 gagttcgggg aaccgttccc ggggaacatt gcccgacatg atggtaactg ccttcccctg
     1861 ctcgggcatc gatatccgtt ccctgaaaat cctgtagagg tgggtatctt ccttgtccag
     1921 gtggatatcg aggtggaaac ggaacgcagc ggttgcccgg gctgtcatga tggggtcgcg
     1981 ggcagcatag gatctgttca gttcctcgaa tgccatgtcc agaccgcggt gatccttcac
     2041 ataggcctcg gcaaccaggg gggcaacctg ctcgaccgcg gggaagatgg cctgctcctc
     2101 cccgtgggca tgccagacca gcacctcgtt catgaaccgg aaacgctcga tggtggcggc
     2161 gagcccctcc tttccccggg ccgattccag cgctgctgca tcgatctgtc tcagatcatt
     2221 cctgaacgca ttgtggatag cccgaattgc atcgacaggt tcggtcatga tgctcccctc
     2281 ccctcccgga gtataaaaac ctgcggaatc cttctacttt ttttcaggtg gaatgggcat
     2341 ccccgtagtg atggatcagg tcatggacga tgatgatgcc gatccccacc cccagcgagt
     2401 tggcgatgat catctgggga atggccgtga ccactatctc cacggcaagg ctgaagggct
     2461 ggacaaggag gagcgccagc ccgccgtgaa gcacctcaat ccctccggca aagaacatcg
     2521 ccggaacaac tccaagcagt ctccttttgt tgaggacata gacggcacct ccgagcaccc
     2581 ccgcgaggac ggttgcgagg gaacagggaa ccgcagttac accacccagg aagaagcggt
     2641 ggatgccccc gatcagcccg tctgccaggc ctgcgtacgg gccagcaacg aggccggcca
     2701 ccatgggtgc gagatccctg atattcacga tggcgccggt actttccgtt gtcccgatgt
     2761 acgtcccgaa gatggagaac aaaccgaaga gcagggtgaa accgacagaa tccgcctttt
     2821 tcgcttcctt gaagatcacc cgctggatgg catcgagttt gacgacgatc tccatgaaca
     2881 cgaagatgaa gatctcggtg ctcgcgaaga gtgccaggta cccgcccagg atgctttgag
     2941 atccctctcc tacggccatg gtccccctgt acccgtatag aacgagtgca gccttaacgg
     3001 tgtctgaacc tacccgatcc tttcatcgga tcagg
//
LOCUS       DATGDI010000179         1929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_76766, whole genome shotgun sequence.
ACCESSION   DATGDI010000179 DATGDI010000000
VERSION     DATGDI010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76766"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VKO45_06640"
     CDS             <1..777
                     /locus_tag="VKO45_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HMA05589.1"
                     /translation="EYTRETFIGHWGRWLIFILLGLPFALMQFVVDPRKIIEGTTIHW
                     ELVPWRTLIAIIIIGIFASIFIAGYVVQIYRGVKPAPAFANWVSLFVEGLKLDIVVFV
                     WFLPGIILGLLALLALVGGVILPGPASRSSYLLPAILIFLVLIIIAMILFVVAALYGT
                     LGAIRFARTGAMTEGWRFSTITAIIRRMGWGNYIIALILLGVASFLFNLVVSIPAIIP
                     YIGWIVPVCLLPLLTVFTARYFMLIYEAGEAPPPAAPAAP"
     gene            840..1544
                     /locus_tag="VKO45_06645"
     CDS             840..1544
                     /locus_tag="VKO45_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HMA05590.1"
                     /translation="MWPELRRRLGRTLSVYRRRKDPSRVGETSVGFVDPATMPVVPVA
                     DKGTIRFVERHLPYRQIHDVGEYRFDDIRREDVVVDIGANAGAFCIRAARYSDHVTAV
                     EPVSWEVLQENIRLNRAPVKIIVGALGDGNPGEVSWDGFRATVQTYTLRQIIGMAGGC
                     DFLKSDCEGAEWCIRPDDLEGIRRIEMELHIPPIGDPPNSALLEYIGRHYDFEIDRTP
                     GHDVMGVMGILHATRS"
     gene            complement(1547..>1929)
                     /locus_tag="VKO45_06650"
     CDS             complement(1547..>1929)
                     /locus_tag="VKO45_06650"
                     /inference="COORDINATES: protein motif:HMM:NF019610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05591.1"
                     /translation="LGITEADLFSPGLNFVFGIANRGRALISTFRLREEFYGPARKPD
                     GFPVRHLPSGPGGREPNATVFRRRVLVESVHELGHALGLDHCEFPGCVMYFSNWIGDT
                     DRKGPGFCFRCARALKRLVETSWA"
BASE COUNT          347 a          603 c          573 g          406 t
ORIGIN      
        1 gagtacaccc gggagacctt catcggccac tgggggaggt ggctgatctt catcctcctc
       61 ggcctgccct tcgccctgat gcagttcgtg gtcgatccaa ggaagatcat cgaggggacc
      121 accatccact gggaactggt accctggcgg acactcatcg ccatcattat aatcggaatc
      181 ttcgcctcca tcttcatcgc ggggtacgtg gttcagatct accgcggggt gaaacccgcc
      241 cccgctttcg cgaactgggt atctctcttc gtcgaggggc tgaaactgga catcgtggtc
      301 ttcgtctggt ttttaccggg gatcatcctc gggctcctcg ccctccttgc cctggtcggt
      361 ggtgtgatcc tgcccggccc cgcctcgaga tcgagctatc tgcttccggc catcctcatc
      421 ttcctcgtgc tgatcatcat tgccatgatc ctctttgtcg tcgccgccct gtacggaacc
      481 ctgggagcca tccggttcgc gcggaccggg gccatgaccg agggatggag attttccacc
      541 atcaccgcca tcatcaggag gatggggtgg gggaattaca tcatcgccct gattctcctc
      601 ggggttgcct ccttcctttt caacctcgtc gtatcgatcc cggccatcat cccctacatc
      661 gggtggatcg tccccgtttg cctcctcccc ctcctgaccg tcttcaccgc ccggtacttt
      721 atgctcatct acgaggccgg cgaggctccg ccgcccgccg ctcctgcggc accataaggg
      781 atatccctct ttccctcact cttctaaaca tcgatcacgg catggaaggt gtggtgcctg
      841 tgtggcctga actcagacgg cgactgggga gaaccctgtc ggtctatcgt cgcaggaagg
      901 acccttccag ggtgggggag acatccgtcg ggttcgtgga ccccgccacg atgcccgtgg
      961 tgccggtggc tgacaaagga actatcaggt tcgtagagcg gcatctcccc tatcgccaga
     1021 tccacgatgt cggcgaatat cgcttcgatg acatcaggag ggaggatgtc gtggtggaca
     1081 tcggggcaaa cgcgggagcc ttctgcatcc gggccgcccg gtattcggat catgtgactg
     1141 ccgtcgaacc tgtaagctgg gaggtgttac aggaaaacat aaggttgaac cgggcgccgg
     1201 tgaagattat cgtcggggct ctcggtgacg ggaaccccgg ggaggtttcc tgggatgggt
     1261 tccgggccac ggtgcagacc tacaccctcc ggcagatcat agggatggcc gggggctgcg
     1321 actttttaaa gagcgattgt gagggtgcgg agtggtgtat ccggccggat gacctggagg
     1381 ggatccggag gatcgagatg gagctccata ttccccctat cggggatcct ccgaactccg
     1441 ccctcctcga atacatcggc cgccactacg actttgagat cgatcggaca cccggccatg
     1501 acgtgatggg ggtgatggga atcctccacg caacccggtc gtgaggtcag gcccagctcg
     1561 tctccaccag cctcttgagg gcccttgcac accggaaaca gaaaccaggt cctttccgat
     1621 ctgtatcccc gatccagttg gagaagtaca tcacgcagcc gggaaactcg cagtgatcga
     1681 ggcccagggc atgccccagc tcgtgcaccg actcgacgag gaccctcctc cggaataccg
     1741 tcgcgttcgg ttcccgtccg cctggcccgg agggcaggtg gcggacggga aaaccatcag
     1801 gttttcgagc cggcccgtag aactcttcgc gaagccggaa ggtcgatatc agggccctcc
     1861 cccggttcgc gataccgaac acgaagttca gtccaggtga gaagaggtcc gcctcggtaa
     1921 tcccgagga
//
LOCUS       DATGDI010000180        11667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_47583, whole genome shotgun sequence.
ACCESSION   DATGDI010000180 DATGDI010000000
VERSION     DATGDI010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47583"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VKO45_06655"
     CDS             <1..191
                     /locus_tag="VKO45_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HMA05592.1"
                     /translation="NKSKGYGKPFYEIFKEAKFDFYKIDTSLFSPAEVVINELTEGVV
                     YHVGAVNPDVTLKSFGFS"
     gene            431..504
                     /locus_tag="VKO45_06660"
     tRNA            431..504
                     /locus_tag="VKO45_06660"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:465..467,aa:Val,seq:tac)
     gene            669..1007
                     /locus_tag="VKO45_06665"
     CDS             669..1007
                     /locus_tag="VKO45_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05593.1"
                     /translation="MAPEKKPVLLFTTYKFRDGTNLSVRRGIQLASKLTMGETVWLQS
                     REGGEQKKALVTGLKVKRFRDIREQDLSVEHDPQCRSWNDLFATMKKLYDDFDEMEIV
                     TMIYFTTEGE"
     gene            1161..2375
                     /locus_tag="VKO45_06670"
     CDS             1161..2375
                     /locus_tag="VKO45_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HMA05594.1"
                     /translation="MEGETDREQIYQEALRLHEEHRGKLEIHSKVPLETRRDLSRAYT
                     PGVAEVCRAIQKDRNLAYRYTLKGNSVAIVTDGSAVLGLGNIGGYAAIPVMEGKAILF
                     KKFANIDAFPICFESYHTGFVDEVKNIAPVFGGINLEDIAAPRCFEVEEALQDIGIPV
                     MHDDQHGTAIVVLAALVNACKVTGKSFEDLEIVVCGAGAAGYAIARQLGCIGYGPNVC
                     RKVRDIVVCDTRGIIHRQREGLYTNKYKFILAEETNRLGKTGTLADAVEGADVLIGVS
                     SPGVFTREMIRSMNDDPIVFAMANPVPEIMPHLAKEAGASVVGTGRSDFPNQINNALA
                     FPGVFRGALDVFATRISEEMKVAAAHALAGYVPEPDREHIVPAVLDREVTMQVAAAVR
                     EAAIVSGCARSI"
     gene            2446..2793
                     /locus_tag="VKO45_06675"
     CDS             2446..2793
                     /locus_tag="VKO45_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMA05595.1"
                     /translation="MAGKKPRENEEKTCNPLTEGQRCAKEGKHEKAIEYFDLAIKEKP
                     DSADAWLAKCNELKAVERYSEALECYATALKIDPKSCRAWIEKGNTLVKVGRGDEALE
                     CFGTARKVATRRK"
     gene            complement(2862..4040)
                     /gene="pscS"
                     /locus_tag="VKO45_06680"
     CDS             complement(2862..4040)
                     /gene="pscS"
                     /locus_tag="VKO45_06680"
                     /EC_number="2.5.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-phospho-L-seryl-tRNA:Cys-tRNA synthase"
                     /protein_id="HMA05596.1"
                     /translation="MKCTGDIEVRDLEELAINIDPIQAGGRLTPEAMKAVIAYGDGYS
                     VCDNCRKPFRLDQIKKPPLDSFHRELATFVGMDEARVMPGARRGFQAVAGTYVEKGSP
                     VLLTALSHYTSFLAVEAAGGVPREIPKDGRNLITADAVAARIDEVTREFGKPPALLFV
                     DHFDYQFGNEHEVRDIARAAHTHDVRVLYNGAYSVGILPVDGRELGVDFVVGSGHKSM
                     ASPAPSGVLATTAEHARDLFRTTRAKGDLTQRQFGVKEVELLGCTLMGATIMGMMASF
                     PAVKERVKHWEEELANGRIVIDALRSIEGTVILSEYPRKHTLTRVDTRDSFDRVAQAH
                     KKRGYFFTSALAEKGIGGVIPGATKVWKFNTYGLTATQCGYLASAFTDIARENSISVA
                     "
     gene            complement(4037..4219)
                     /locus_tag="VKO45_06685"
     CDS             complement(4037..4219)
                     /locus_tag="VKO45_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05597.1"
                     /translation="MSRRAVDAVFQGLFTLTDIRVILRRTAPLHRLSGADRSLVQGQI
                     EDLRRQIGIIEEELLR"
     gene            4342..5493
                     /locus_tag="VKO45_06690"
     CDS             4342..5493
                     /locus_tag="VKO45_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMA05598.1"
                     /translation="MRFVHIGDSHLGLSQFSRLAGDGVNLREGLVYGNFLGGIDAIIA
                     MKPEAVIHSGDLFNSVRPKTLAYTTALESLSRLGNAGIPLIVIAGNHDMRKSRYTTSP
                     FEVLEYHRAPLHAGYGSHYEQVDLGDSRFHLIPNLLEPGQYREEFDQVEPLRGGCNVL
                     VTHGLASTLKDRRLGTVMEHEIDTTMLSPKLDYIALGHFHGQKQVADNAWYCGSQEYI
                     TYGEIHDTKGGLLVDLDRHTVDHVDLPCTPMVDLGELQCSSLSSREVGEAILGKVPEA
                     TSSGQPVMAIVTLTGTDRRLLQSVDPEILQEARSRVLDLRIAVRTETSETPLSGRRDL
                     TGVNYVDLFREYLEGRNLGKKESEFVLGKGGEVIRRVMAGDAGEDDAAP"
     gene            5480..8284
                     /locus_tag="VKO45_06695"
     CDS             5480..8284
                     /locus_tag="VKO45_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HMA05599.1"
                     /translation="MQLLRLQMRNFRRYRHQDVVFRDGITGILGNNGTGKSTIVDAIL
                     FCLYGVRETGLDYILSATAGRKDRAEVRLDFSVRGGEYQVVRSLGPQKRHDAQLNHNG
                     RLMAKGVSEVQEALKRIIRMGHADFRHTIFSGQRELLILVEATPEERKRWFRRVLGID
                     SLKDEGGEILRSDAGAARERLLQIEGRLREIDPLAIQGDLEDLGKRITDSGEKLMSLD
                     EDLRSAADRRGSLEKAVSELRVLEKEALRLRTLLQSRDGDRVRMVEELAGISREMEAL
                     GLQRGEFQDLAASEPGFEALRERLEESRARSYRFQALQTDEVRTKALISDGQETLDRL
                     RHEEASLGEADARLESLKPRVARRAEIQGRMQEIGSIEERYHALMAEKAKKAEALSGA
                     AERGATLRARIGKAKEARERLASLAEPYRSRVSLPGDPVAGLESLRRELLERKAGSSA
                     TRDQTAKALKKMADDLAALEARGTEGDCPTCHQPLGPRYRQIMEAVRRDMEGEKVRVS
                     EAEKALKVTDAELSALETALTEAHRLREVSNRMEELTAEWEAIQDLSRVNLAVKDRLE
                     RDLQALAFEPAERQRLETELARLEEAWGEHVALSERVKRRPSLARDIRDALGRLEQQK
                     ETLAALRRDREESGFDPEIHARLEKEFRIAEERHRRYLAMKPDLERLPHLEERARGLA
                     GEVAGVKEDLARLAAELQSIPFSPEILARKEVEIRAAQEQALRTAEEATRCRADLVHL
                     GEEKKRLEGLLRKSGLDRAEHDRLGEEIRLLELTRDQLNGFTDHLLGIVRDQIQEETG
                     RILSEITDGRYDTVILDDSFEILVHDLGGDYPVARFSGGEQDDVAIALRIALSRYIAE
                     MHELHDSTFLIFDEIFGSQDEERRANIFRSLRALEPYFPQIFLISHVTEVQGEFGNTL
                     LVEAVSGNESRIRDLEEAAA"
     gene            8281..9309
                     /locus_tag="VKO45_06700"
     CDS             8281..9309
                     /locus_tag="VKO45_06700"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HMA05600.1"
                     /translation="MTIDEEYRRDLEVLVGNVGDLGPPDLHSRFASAFDLNPESFRAI
                     RPEQRGTIAAVDGSHALLLDAGCFQVAVLRAAACGFSDGARRFRSVTPLRALTLGRES
                     RDGSFEGLYRECFGTDPEATVRDDEPERTAGIMRDTLEYRAALEAAGRLKRGDLLLLD
                     GALRISHASHEPVILDLIRACAKKGVQLCAVTKRTAITWGGGLPLVPAALSLAKAWSI
                     PPPWVLRIGEEGLDVTPYRQWRHGVPCVAALHPASPLAFKIEVPEPTGEDTLKEIVSR
                     LAGWSDDGRVTGYPYPLLDAHRTARIPADALQKVKNDLLAGMGRSGLDPRLFQDLFGD
                     IHDEFARY"
     gene            9287..10963
                     /locus_tag="VKO45_06705"
     CDS             9287..10963
                     /locus_tag="VKO45_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HMA05601.1"
                     /translation="MTSLHDTDLGDPSKYRLIGNSVLSYRFAIPYDETLYVGNILKIE
                     DLAKEMTFFARVTDLRHEANFDDGKWDMRAHSEHFYGLSEDVYLVVEALPLGYVDGAG
                     IFRRPRTLPTKFSRVLVPSAPDFRFLSQVMGDVEVGSLKSGQEVLRDVRVALHSRVMP
                     QHMGVFATTGMGKSNFMKVFCASSMRARKFGLLLVDPHGEYVAGGVSATGLPTEGLVH
                     YQAGRDGLSVYTIREESFRRRYSLSSLYLENDDFRATDLLLLFDHSSAQRELVEMIDD
                     VPGSELVDLFLNTEFDENGDWEATTPYRKTAGRLAQFQPSTLQVMKRRVGILVKSNPF
                     LRAKGSSVPDILADLAKNRVVLIDIPGMGERSELFVLSVLARKILDRHRGEASGWGRT
                     DFPERREVLITIEEAQRVLGAGGPGTAVFRECAMEGRKFGVGLCVVTQQPRNIDPRVL
                     AQMNTFVVMGLSDRNDRAIVAGHAKQDLSPMDTEIQTLEPGEAIISTLGIPFPVSTRI
                     HLFEEYIASLRGKEGGPGSQGAAGPGSKGAAGPGSKGAGGSLREGLENRF"
     gene            complement(10970..>11667)
                     /locus_tag="VKO45_06710"
     CDS             complement(10970..>11667)
                     /locus_tag="VKO45_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMA05602.1"
                     /translation="GRFRQSVESFDRVLAPEPRHPGALVARASGLSRMGYFQEAVKCC
                     EEVIGRDPLDPGAWFIRGSVLYMTGRWTEARPCLDRATAIDPEYAEAWYTLGNCHYYL
                     GDYEDALCSYDAVIGLDPEYPKAWYNKGVALADVHRYGEALACYEECARINPGVAVVY
                     TNMGVALAGLNRFGEAIARFDEAIGINPHDPTPWNNKAASLRRLGREEDAEACAEKVR
                     ELTRGREIPWIIR"
BASE COUNT         2210 a         3656 c         3802 g         1999 t
ORIGIN      
        1 gcaacaagtc caagggctac gggaagccct tctacgagat cttcaaggag gccaagttcg
       61 acttctacaa gatcgacacc tccctcttct ctcccgccga agtggtcatc aacgagctca
      121 ccgaaggcgt cgtgtaccac gtgggagccg tgaaccccga cgtgaccctg aagtcatttg
      181 gattctcata gaatctgaat ggtgactccg aagtgagtca cccctttcgg gtttcagtga
      241 aacccggaaa gtgatggcaa agccatcact ccttcgggtt cctatagaac ctggagggtg
      301 actccgaagt gaaccactcc ttcgggttcc agtaaccttt tccttctctt tccttctgcc
      361 ctgccgccgt ggaacgggaa ggcggtcctt tgagcaattt taataggatc gccgctccac
      421 cctaactggc gggcttgtgg tctagctggt catgatatcg cctttacaag gcggggatcg
      481 tcggttcgaa tccgaccaag cccatggacg ttcttttcac gcggcaaacc cggtcgtgcc
      541 tgcctgcagg ttgtgatgtg cgtcgtaagc caccggtgag gcccggagtg aattatcagc
      601 cggaaagcac tacccttatt acctgcgata ccgagaagct ccctagagac acgaggctgg
      661 aacactccat ggctcccgaa aagaaacctg tcctcctctt cacgacctac aaattccgtg
      721 acgggacgaa cctcagcgtg cgacggggaa tacagctggc ctcgaaactg accatggggg
      781 aaaccgtctg gctgcagtca cgggagggag gggaacagaa aaaggccctc gtgaccggtt
      841 tgaaggtgaa gcggttccgg gatatccggg aacaggatct gtccgtggaa catgatcccc
      901 agtgccggag ctggaacgat ctctttgcca ccatgaagaa gctctatgac gatttcgacg
      961 agatggaaat cgtcaccatg atttatttta cgaccgaggg ggaataacac ccccggcccg
     1021 ggagggaagt ctcccttccg ccggggaatc ccaccccggt atcgagggaa tcccgcggtt
     1081 ttccccggaa tgtaccgttc ctgcgaggga tcgaaacgaa tttgatacca ggaaccccgg
     1141 atcaggtggg ggcatgagtc gtggaagggg agaccgatag ggagcagatt taccaggaag
     1201 cactcaggct gcacgaagag caccggggaa aactggagat ccactcgaag gtccctctgg
     1261 agaccaggcg cgatctctcg cgggcatata cccccggggt cgcggaggta tgccgggcca
     1321 tccagaagga caggaacctc gcctaccggt ataccctcaa ggggaattcg gtggccatcg
     1381 tgaccgatgg atccgccgtc ctgggcctgg ggaatatcgg cggctatgcg gccattcccg
     1441 tcatggaggg caaggcgatc ctcttcaaga aatttgcgaa tatcgatgcc ttccccatct
     1501 gcttcgagag ttaccacacc ggcttcgtcg acgaggtgaa aaacatcgcc ccggttttcg
     1561 gggggatcaa cctcgaggat atcgccgcac ccaggtgttt cgaggtggag gaggccctcc
     1621 aggacatcgg gatcccggtg atgcatgacg accagcatgg aacggcgatc gtggtgctcg
     1681 ccgcccttgt caatgcctgc aaggtcacag ggaagagctt cgaggacctg gagatagtgg
     1741 tgtgcggtgc cggtgctgcg gggtatgcca tcgcgcgtca gctggggtgc atcgggtacg
     1801 gtcccaatgt ctgccggaag gtccgggata tcgttgtctg tgacacccgg ggaatcattc
     1861 accggcaaag ggaggggctc tacacgaaca aatacaagtt tatcctcgct gaggagacga
     1921 atcgcctggg gaagacggga accctcgccg atgccgtgga gggagcggat gttctcatcg
     1981 gggtctcttc tccgggggtg ttcaccaggg aaatgatccg ctcgatgaat gacgatccca
     2041 tcgtgtttgc catggcaaat cccgtcccgg agatcatgcc gcacctggca aaggaggctg
     2101 gtgcatcggt ggtagggacc ggcaggagcg attttcccaa ccagatcaac aatgcccttg
     2161 ccttccccgg ggtcttcagg ggagccctgg atgtgtttgc cacgaggatc agcgaggaga
     2221 tgaaggttgc tgctgcccat gctctcgccg ggtatgtgcc agagcctgac cgggaacaca
     2281 tcgtccctgc cgtccttgac cgggaggtca ccatgcaggt ggcggctgcg gtgagagagg
     2341 cggcgattgt ctccgggtgt gcgaggagta tttaatcccc ggacgaaggc attcactcac
     2401 cccgacgaac ccaaatctca tcatctctcc gccccaccat tgatcatggc cggcaaaaag
     2461 ccgcgagaga acgaggaaaa aacctgcaac ccgctcacgg aggggcagcg gtgtgcgaag
     2521 gaggggaagc acgagaaggc catcgagtac ttcgacctgg ccatcaagga aaaaccggat
     2581 tcggccgatg cctggctcgc gaagtgcaat gaactgaagg ccgtcgagag gtactccgag
     2641 gcgctggaat gctatgccac cgctctcaag atcgacccga agtcctgccg ggcctggatc
     2701 gagaagggga acaccctcgt gaaggtcggc cgcggagacg aggccctcga gtgcttcggg
     2761 acggcgagga aggtggcaac caggcgaaag taatggttgc ccccgacgga gggaagcatt
     2821 ttgaagggga ggcctggttc ccccgaatcc tgccggcctg atcaggcgac cgaaatactg
     2881 ttctcccggg cgatgtccgt aaacgccgac gcgaggtacc cgcactgggt cgcggtgagg
     2941 ccgtaggtgt tgaacttcca gaccttcgtc gccccgggga tgaccccccc gatacccttt
     3001 tcggcaaggg cactcgtgaa gaaatatccc cgcttcttat gggcctgggc tacccggtca
     3061 aaggagtccc gggtgtccac ccgggtcagg gtgtgttttc tcgggtactc ggagaggatg
     3121 acagtcccct cgatggaacg gagtgcgtcg atgacgatcc tcccgttcgc gagctcctcc
     3181 tcccagtgct ttacccgttc cttcaccgcc gggaaggagg ccatcatccc catgatggtc
     3241 gcccccatga gggtacagcc caggagctcc acctccttca ccccgaactg gcgctgggtc
     3301 aggtcgccct tcgcccgggt cgtccggaag aggtcccggg cgtgctccgc ggtggtggcg
     3361 agaacgccgg agggagcggg agaggccatg ctcttgtggc ccgagcccac cacgaagtca
     3421 acgccgagct ccctcccatc gacgggaagg atccccaccg aataggctcc gttgtagagc
     3481 acccggacat catgggtatg ggcggcccgg gcgatgtccc gcacctcgtg ctcgttcccg
     3541 aactggtagt cgaagtggtc gacgaaaagg agggccggag gcttcccgaa ctcccgggtc
     3601 acctcgtcga tccgtgcggc cacagcatcg gcggtgatga ggttcctccc gtccttcggt
     3661 atctcccggg gaacccctcc cgccgcctcc acggcgagga aactcgtgta atgggaaagg
     3721 gcggtgagga ggaccggtga ccctttctcg acgtaggtgc cggcaacagc ctggaagccc
     3781 ctccgggcgc cgggcatgac ccgtgcctcg tccatcccca cgaaggtcgc gagctcccgg
     3841 tggaaactgt cgaggggggg cttcttgatc tggtcgagcc ggaagggctt ccggcagttg
     3901 tcgcagaccg agtaaccgtc accataggcg atgacggcct tcatggcctc cggggtgagc
     3961 cttccccccg cctgtatggg gtcgatgttg atggccagct cctcgaggtc ccggacctcg
     4021 atgtcccccg tgcatttcat cgaaggagct cctcctcgat gatcccgatc tgccggcgca
     4081 gatcctcgat ctggccctgc accagactcc ggtccgcccc ggagagccgg tggagggggg
     4141 cggtccgccg gaggatcacc cggatatcgg tgagggtgaa gaggccctgg aagaccgcat
     4201 cgactgctct tcgcgacata gcatcagcat aagcatatca gggaacgagt atagatagct
     4261 ggtgatcggg gggtttcttc agattcacgc cgcgagcacg agctctataa aggcagggga
     4321 tgatctccgg gcaggggaga gatgaggttc gtccatatcg gtgacagcca tctgggcctc
     4381 tcgcagttca gccgcctggc cggcgatggg gtgaacctcc gggagggcct ggtctacggg
     4441 aacttcctcg gtggtatcga cgccatcatc gcgatgaagc cggaggcggt gatccattcg
     4501 ggggatctct tcaactcggt caggcccaag accctcgcct acaccaccgc cctcgaatcc
     4561 ctttcccggc tggggaatgc ggggatcccc ctcatcgtca tcgcggggaa tcatgacatg
     4621 aggaagtccc ggtacaccac ctcccccttc gaggtgctcg agtaccaccg ggcgcccctc
     4681 catgccgggt acggatctca ttacgaacag gtcgacctgg gcgacagccg gttccatctc
     4741 atcccgaacc tcctcgagcc cggccagtac cgggaagagt tcgaccaagt ggagccgctc
     4801 cggggaggat gcaacgttct cgtcacccac gggctcgcga gtactctcaa ggatcgccgg
     4861 ctggggacgg tcatggagca tgagatcgat acgaccatgc tctccccgaa gcttgattac
     4921 atcgccctcg gccactttca tggccagaaa caggtcgcag acaatgcatg gtattgcggg
     4981 tcccaggagt acatcaccta cggcgagatc catgatacca agggcgggct cctcgtggac
     5041 ctggacaggc acacggttga ccacgtagac ctcccgtgca cccccatggt agatcttgga
     5101 gagctccagt gctcctccct ctcttcccgg gaggtaggag aggcaatcct cggcaaggtc
     5161 ccggaggcca cgagctccgg gcaacccgtc atggccatcg tcaccctcac gggaacagac
     5221 cgccggctcc tccagtcggt ggacccggag atcctccagg aggcaaggtc ccgagtcctc
     5281 gatttgagga tcgccgtccg aaccgaaaca tcggaaaccc cgctcagcgg acggcgggat
     5341 ctcaccggcg tgaattacgt ggatctcttc agggaatacc tggagggccg gaacctcggg
     5401 aagaaggaga gcgaatttgt cctcgggaag ggaggagagg tgatccggag ggtcatggcc
     5461 ggtgacgcag gtgaggacga tgcagctcct taggctgcag atgcggaact tccggcgata
     5521 ccggcaccag gacgtggtgt tcagggacgg gatcaccggg atcctcggga acaacggcac
     5581 gggcaagagc accatcgtcg atgccattct cttctgcctc tatggcgtgc gggagaccgg
     5641 gctcgattac atcctgagcg ccacggcggg ccggaaggat cgggccgagg tacggctgga
     5701 cttctcggtc aggggagggg agtaccaggt tgtccggtcc ctcgggccgc agaaaaggca
     5761 cgatgcccag ctgaaccaca acggccggct catggcgaag ggggtctccg aggtccagga
     5821 ggccctgaaa cggatcatca ggatgggcca tgccgacttc aggcatacca tcttctcggg
     5881 gcagagggag cttctcatcc tcgtcgaagc aacccccgag gagcggaaga gatggttccg
     5941 gagggtgctc gggatcgaca gcctgaagga cgagggcggg gagatcctcc ggagtgacgc
     6001 gggtgccgcc agggaacggc tcctccagat cgagggaagg ctccgcgaga tcgaccccct
     6061 ggcgatccag ggggatctcg aggatctcgg gaagcggatc acagacagcg gcgagaaact
     6121 catgtctctc gacgaggatc tgaggagtgc cgcggatcgc cgggggtctc tcgagaaggc
     6181 tgtctcggaa ctccgtgtcc tcgaaaagga ggcactccgt cttcgcaccc ttctccagag
     6241 ccgtgacggt gaccgggtga ggatggtgga ggagctcgcc gggatctccc gcgagatgga
     6301 ggcccttggg ctgcagaggg gggagttcca ggacctcgcc gcatccgaac cgggattcga
     6361 ggccctcagg gaacggctcg aagagagcag ggcgcggagc taccgcttcc aggcgctcca
     6421 gaccgatgag gtccggacga aggccctcat ctccgatggg caggaaaccc tcgaccggct
     6481 ccggcatgag gaggcctccc ttggagaggc cgatgcgagg ctcgaatccc tgaaaccccg
     6541 ggttgcacgg cgtgcggaga tacagggcag gatgcaggag atcgggagca tcgaagagcg
     6601 gtaccatgcc ctcatggcag agaaggccaa gaaggccgag gcactgtcgg gggcggcgga
     6661 acgaggggca acgctccgtg cccgcatcgg gaaggcgaag gaggcccggg agcgcctcgc
     6721 ctccctcgcc gaaccctatc gttcccgcgt ttccctgcca ggggacccgg tcgccgggct
     6781 ggaatcactc cgccgcgagc tcctggagcg gaaggccggg agctccgcca cccgggacca
     6841 gacagcaaaa gccctcaaga aaatggccga tgacctcgcg gccctggaag ccaggggcac
     6901 cgaaggggac tgtcccacct gccaccagcc cctcggtccc cggtaccggc agatcatgga
     6961 ggccgtccgg agggatatgg agggagagaa ggtcagggtc tcggaggcag aaaaggccct
     7021 gaaagtgacc gatgcagagt tatccgccct cgaaactgct ctcaccgagg cacaccggct
     7081 ccgtgaggtc tcaaaccgga tggaggagct caccgccgaa tgggaggcga tccaggatct
     7141 ctcccgggtg aacctcgccg tgaaggaccg gctggagcgg gatctgcagg ctctcgcttt
     7201 cgagccggcg gagaggcagc ggctcgagac ggagcttgcc cgcctcgagg aggcctgggg
     7261 ggagcatgtc gccctgtcgg aacgggtgaa gcggcgcccg tcccttgccc gggatatccg
     7321 ggatgccctg ggacggctgg agcagcagaa ggaaaccctt gcggcgctcc gccgcgaccg
     7381 ggaggagtcg gggtttgatc ccgagattca tgcccggctg gagaaggaat tccggatcgc
     7441 ggaggagcgg caccggcggt atctcgcgat gaaacccgat ctcgagcggc tcccgcacct
     7501 cgaggagcgg gcccgcggcc tcgccggtga ggttgccggg gtgaaggagg atctggcgcg
     7561 actggcggcc gaactgcaat cgatcccgtt ttcgccggag atcctcgccc ggaaggaggt
     7621 ggaaatccgg gcggcgcagg agcaggcact caggaccgca gaggaggcga cgaggtgccg
     7681 ggcggacctg gtccacctcg gggaagaaaa gaaacgcctc gagggtctcc tccggaagtc
     7741 cgggctcgac cgggcggaac acgaccggct cggggaggag atccggctcc tggaactgac
     7801 ccgcgaccag ctgaacggct tcaccgacca cctcctcggc atcgtccggg accagatcca
     7861 ggaagagacg ggccgcatcc tctcggagat caccgacggg cggtatgaca ccgtcatcct
     7921 cgacgacagc ttcgagatcc tcgtccatga tctgggcggc gactacccgg tggcccggtt
     7981 ctcgggcggg gagcaggacg acgtggccat cgcgctccgc atcgccctct cccggtacat
     8041 cgccgagatg cacgagctcc atgacagcac cttcctcatc ttcgacgaga tcttcgggag
     8101 ccaggacgag gagcggcggg cgaatatctt ccggtccctg agagccctcg aaccctattt
     8161 cccccagatc ttcctcatct cccatgtcac cgaggtgcag ggggagttcg ggaacaccct
     8221 cctcgtcgag gccgtgtcgg ggaacgagag ccggatccgg gacctcgagg aggccgccgc
     8281 atgaccatcg acgaggaata ccggagggat ctcgaggtcc tcgttggaaa cgtcggcgat
     8341 ctcggtcccc cggacctgca ctcccggttt gcctctgcat tcgatctcaa tcccgagtcc
     8401 ttccgggcga tacggcccga gcagcggggg accatcgctg ccgtagacgg gtcccatgcc
     8461 ctgctgctcg atgccggctg cttccaggtc gccgtcctcc gggccgcagc ctgcgggttt
     8521 tcggatggcg cccgcaggtt ccggtccgtg acccccctcc gggccctgac cctcgggcgg
     8581 gaatcccgtg acgggagttt cgagggattg taccgtgagt gtttcggcac cgatcccgag
     8641 gcgacggtga gggacgacga accggagagg accgccggca tcatgaggga tacgctggaa
     8701 taccgggccg ccctcgaggc cgccgggcgg ttgaaacgag gggatctgct cctcctcgac
     8761 ggggcgcttc ggatctccca tgcgagccat gaaccggtga tcctcgacct catccgtgcc
     8821 tgcgcgaaga agggtgtcca gctgtgcgcg gtgaccaaga ggacggccat cacctggggc
     8881 ggggggctcc ccctggtgcc cgcggccctc tccctcgcaa aggcctggtc cattccaccg
     8941 ccctgggtcc tcaggatcgg agaggagggg ctcgatgtca ccccgtaccg gcagtggagg
     9001 cacggggttc cctgcgtcgc cgcccttcac cccgcctcac ccctcgcctt caagatcgag
     9061 gtacccgaac cgaccgggga ggataccctg aaggagatcg tctcccggct tgccggctgg
     9121 tcggacgatg gacgggtgac cggctatccc taccccctcc tcgatgccca tcggacggcc
     9181 cggatccctg ccgacgccct ccagaaggtg aagaacgatc tgctcgccgg catggggcga
     9241 tccggcctcg atccccggct cttccaggac ctgtttggtg atatccatga cgagtttgca
     9301 cgatactgat ctcggcgatc cctccaagta ccggctcatc gggaactcgg tcctctcgta
     9361 ccggttcgcc atcccctatg acgagaccct ctacgtggga aacatcctga agatcgagga
     9421 cctcgcgaag gagatgacct tcttcgcccg ggtcaccgat ctcaggcacg aggccaactt
     9481 cgatgacggg aaatgggaca tgcgggcgca ctccgaacac ttctacgggc tctcagagga
     9541 tgtgtacctg gtggtggagg cgctccccct cggatatgtc gatggggcgg gcattttccg
     9601 ccgcccgagg accctcccca ccaagttctc ccgggtcctc gttccctcgg ccccggattt
     9661 ccggtttctc agccaggtca tgggcgacgt ggaggtgggc tccctcaagt ccgggcagga
     9721 ggtgctccgg gacgtgaggg tcgccctcca cagccgggtc atgccccagc acatgggggt
     9781 ctttgccacg acgggcatgg gcaagagcaa cttcatgaag gtcttctgcg cctcctcgat
     9841 gagggcgagg aagttcgggc tcctcctcgt cgatccccac ggcgaatacg tggccggcgg
     9901 ggtctcggcc accggcctgc cgacggaggg cctggtccac taccaggccg ggagggacgg
     9961 cctctcggtc tataccatcc gggaagaatc cttccggcgc cgttactcgc tctcaagcct
    10021 gtacctcgag aatgacgact tccgggccac ggacctgctc ctcctcttcg accactcctc
    10081 ggcgcagcgc gagctcgtcg agatgatcga cgacgtgccg gggagcgagc tcgtggacct
    10141 cttcctgaac accgaattcg acgagaacgg ggactgggag gcgaccaccc cctaccggaa
    10201 gacggcgggg aggctcgccc agttccagcc gagcaccctc caggtgatga aacgccgggt
    10261 aggaatcctc gtgaagtcca atccctttct ccgcgcgaaa gggtcgtccg tcccggatat
    10321 cctcgccgat ctggccaaga acagggtggt cctcatcgac atacccggca tgggggaaag
    10381 gagcgagctc ttcgtcctct cggtgctcgc ccggaagatc ctggaccgcc accggggcga
    10441 ggcatcggga tggggtcgga cggacttccc agaacggagg gaggtgctca tcaccatcga
    10501 ggaggcccag agggtcctcg gcgccggggg cccgggcacc gcggtgttcc gggagtgcgc
    10561 catggagggg aggaagttcg gcgtgggcct ctgcgtcgtg acccagcagc cgaggaacat
    10621 cgacccccgg gtcctcgccc agatgaacac cttcgtggtc atgggcctct cggaccggaa
    10681 cgaccgcgcc atcgtcgcag gccatgcaaa gcaggatctc tcccccatgg acacggagat
    10741 ccagactctc gagcccggcg aggccatcat ctccaccctg ggcatcccct tcccggtctc
    10801 cacccggatc cacctcttcg aggagtatat cgcttctctc cgggggaagg aggggggtcc
    10861 cggttctcag ggggccgcgg gtcccggttc taagggggcc gcgggtcccg gttctaaggg
    10921 ggccggggga tccctccggg aggggctcga gaaccggttc tagggatccc taccggatga
    10981 tccaggggat ctcgcggccc cgggtgagct ccctcacctt ctcggcgcag gcctcggcat
    11041 cctcctctct cccgagcctc cggaggctcg cggccttgtt attccaggga gtcgggtcat
    11101 gggggttgat cccgatggcc tcgtcgaacc tcgcgatggc ttccccgaac cggttcaggc
    11161 cggcgagggc gacccccatg ttcgtgtaca ccaccgcgac cccgggattg atccgggcac
    11221 actcctcgta gcaggcgagg gcctccccgt accggtgcac atcggcgagg gcgacgccct
    11281 tgttgtacca tgccttcggg tactcggggt cgagcccgat gaccgcgtcg taggagcaga
    11341 gggcatcctc gtaatcgccc aggtagtagt gacagttccc gagggtgtac caggcctcgg
    11401 cgtactccgg gtcgatggcc gttgcccggt ccaggcaggg tctggcctcg gtccaccggc
    11461 ccgtcatgta gaggacgctc ccgcggatga accatgcccc gggatccagg ggatccctgc
    11521 cgatgacctc ctcgcagcac ttcaccgcct cctggaagta ccccatcctc gagaggcccg
    11581 aggcccgggc cacgagggcg ccggggtgcc ggggctccgg ggcgagaacc cggtcgaagc
    11641 tctccaccga ctgcctgaac cggccca
//
LOCUS       DATGDI010000181         6005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_338443, whole genome shotgun sequence.
ACCESSION   DATGDI010000181 DATGDI010000000
VERSION     DATGDI010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338443"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /gene="mutL"
                     /locus_tag="VKO45_06715"
     CDS             complement(<1..1209)
                     /gene="mutL"
                     /locus_tag="VKO45_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HMA05603.1"
                     /translation="MAEPEGTPTIRLLDPSEAMKIAAGEVVERPASVAKELVENSLDA
                     GATRIRVEVESDPKRITRITVTDDGAGMNRADARLAFLPHATSKIRSLTDLDRTVSLG
                     FRGEALASIAAVARVTLVTRCHGEECKTGTRVLASGGEIQKVGETGAPPGTSVTVEDL
                     FFNTPARQKFLKSLQTELAQLAGVLEALAVSHPSVTLQFIHNGRERLATHRAPGLLEA
                     VRAVFGDEVAADLIPLSAEDALVRVAGYISTPFSPRKTPSRVLVSINGRPVFSRQILR
                     AVRRGYGTLLPSHTYPAAFLDLGIDPSRVDVNVHPTKRHVRIGEEHEILAVITEAVRE
                     SLAREDLTPPAGGGAPVRSPAAPIDRPVRGPAPVSRGEIPLPTKPVPAVSRLTDRRLR
                     QTELPTGVAGE"
     gene            complement(1202..3868)
                     /gene="mutS"
                     /locus_tag="VKO45_06720"
     CDS             complement(1202..3868)
                     /gene="mutS"
                     /locus_tag="VKO45_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HMA05604.1"
                     /translation="MGRAGNSTGEGDGERLTPAMEQYRSFKEQNPGAILFFHIGDFYE
                     TFGPDAELVSRELDIALTSRSRDRSGNRIPLAGVPHDAAEGYIGRLVAKGYRVAVCDQ
                     VEDSSISRGIVRRAVTRVITPGTADAPGTGELMGSRYLAGVWADGKGDRAGLAFLDTT
                     TGEFLASVAGEAEGLGDIRSEIVRYRPAEAVIPPDLPVRLKAMLEAEGVILTVFRDEA
                     FGPDTAARALLDHFHVDTLAGFGFDPYPEAVRAAGAALRYASETMHSPLGHVTGLSVR
                     VPSRAMLIDAITLRNLEIFEGIRSREKEGTLFSTLDLTMTAMGRRELRRRLAAPLISV
                     GAIGARLDAVEFFMGDAVLCRELRTLLARCADVERIAGRIATGSAGPRDLLALRDLLA
                     VVPGIRALLGKAATLPAPVRESLEGLGDLHDAAALITRGISDDPPASVRQGGIIREGY
                     SPDLDGLRSLSRSVKDWVVELQQKERERTGIRSLRVGYNQVFGYYIEVTKANLHLVPP
                     GYARKQTTAGGERFITGELAEKGDQIASADERILALESELYLSLVRSLQAEIPRFQAL
                     ARSLAGLDVASALAEVARVNEYTRPVLDESLKLAIREGRHPVVEQVLGGRFVPNDTLM
                     DGNGDQILIITGANMAGKSTYMRAVALIAILAQTGSFVPAAHATVGIVDRVFTRVGAF
                     DDLASGQSTFMVEMLELANILNSMTERSLVVLDEIGKGTGTLDGYAIARSVLEFLHGK
                     AAPGPRTLFATHFHEIVGVEAELRRVRNYHFTVKETGSEVVFLRRLVPGATDRSYGIH
                     VARRAGIPRKVTDRAAEILDSARKREDGKEGRPKRYTQVLFVDSPETERGEHPVVKKL
                     RELEPDGMTPLQALEALYALRKQAREEGEKNG"
     gene            4294..5154
                     /locus_tag="VKO45_06725"
     CDS             4294..5154
                     /locus_tag="VKO45_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004198 - calcium-dependent cysteine-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C2 family cysteine protease"
                     /protein_id="HMA05605.1"
                     /translation="MVTKYGYNPSWFYRNTVSGLDPVQGSCNDCYLIAALGAVAWTCP
                     GQIRIPDPSGNISVHFWDTGRLAWSNWFTAAKTESLPVDESGNLFGARSMDANETWPA
                     VYEKLYALFRGVSPSDGGPDIAGNMPDGNALECLSALTGWKTVSLLTSSYSPDALWTA
                     LKPRTSGSKTMFPMVAWTYPNLPALQPGTAIMPKHSFPVLGIYTDAGGSNYLVLRNPR
                     GSVTRNSGISMLDSGSWTFTETIFSAPGVAMAFPRPVPRTLSFNLGQGIFALAMKEVP
                     AYFEGIGYVT"
     gene            5215..>6005
                     /locus_tag="VKO45_06730"
     CDS             5215..>6005
                     /locus_tag="VKO45_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05606.1"
                     /translation="MRKGRMGWCLLAVAFLLPLILLPALAAECTITPDVQSPQAYPDI
                     SGDTVVWLDQGSSDLYLFSLSEGNETRLTSDGMLKASPLISGRLVVWLEGYNLILLNL
                     TTGGTSTLSTDAFTAAISGDLIAFVNSTGVSLYNATSGDSSAVAGEPYDQIQAVISGN
                     RVIWVNGTDSGNVTVGVDIFMRDLTTDTENLVAETLCAANPLGICIMDPQISGERIVW
                     SDNRDNPYTDVYLLDLSAPLERRIVQTPSSSPKPAIDGDLVAWVD"
BASE COUNT         1003 a         2014 c         1920 g         1068 t
ORIGIN      
        1 ttcaccggct acgcctgtcg gaagctccgt ctgccggagc ctcctgtccg tgagccgcga
       61 gacggccggg accggtttcg tcggcagggg aatctccccc cgggacaccg gcgccggccc
      121 ccggacaggc cgatcgatgg gggcggcggg actccggacc ggtgcccccc caccggccgg
      181 cggggtcagg tcctccctcg cgaggctctc cctgaccgcc tccgtgatga cggccaggat
      241 ctcgtgctcc tcgccgatgc ggacgtggcg cttggtggga tgcacgttca cgtcgacccg
      301 cgaggggtcg ataccgagat cgaggaaggc ggccggatag gtgtgggaag ggaggagggt
      361 cccgtacccg cgccggacgg ccctcaggat ctgccgggag aagaccgggc ggccgttgat
      421 ggagacgaga acccgggagg gggtcttccg gggagagaag ggggtggaga tatacccggc
      481 gaccctgacc agggcgtcct ccgccgagag agggatgagg tccgcggcaa cctcgtcccc
      541 gaagaccgcc ctcacggcct ccaggaggcc gggtgcccgg tgggtggcga ggcgttcccg
      601 gccgttgtgg ataaactgaa gggtaaccga gggatgggag acggcgaggg cctcgagaac
      661 ccccgcaagt tgggcgagct cggtctggag ggacttgagg aacttctgcc tggcgggtgt
      721 gttgaagaag aggtcctcca cggtgaccga ggtccccggc ggggccccgg tctcccccac
      781 cttctggatc tccccgccgc tggcgagcac cctcgtcccg gttttgcatt cctcgccgtg
      841 gcacctggtg acgagggtca cccgggcaac ggccgcgatg ctcgcgaggg cctccccccg
      901 gaacccgagg gagacggtgc ggtccaggtc agtgagggag cggatcttgc tcgtggcatg
      961 gggaaggaag gcgagccggg cgtcggcccg gttcattcct gccccgtcgt cggtgacggt
     1021 gatccgggtg atccttttcg ggtccgattc cacctccacc cggatccggg ttgcgccggc
     1081 atccagggag ttctcgacaa gttccttcgc caccgaggcg gggcgttcca ccacctcccc
     1141 cgcggcgatc ttcatcgcct ctgaggggtc caggaggcgg atcgtcgggg ttccctcggg
     1201 ctcagccatt cttttccccc tcctcccgcg cctgcttccg gagggcatag agggcctcga
     1261 gggcctggag gggcgtcatc ccgtcaggct ccagctcccg gagctttttc accaccgggt
     1321 gttctcccct ctccgtctcc ggcgagtcca cgaagagaac ctgggtgtat cgcttcggcc
     1381 tgccctcctt cccatcctcc cttttccggg ccgagtcgag gatctccgcc gcccggtcgg
     1441 tgacctttcg ggggatcccc gcccgccggg cgacatggat accgtagctc cgatcggtcg
     1501 cgccggggac gagcctcctt aagaacacaa cctcgctccc cgtctccttc acggtgaaat
     1561 ggtagttcct caccctcctg agctcggcct ccacccctac gatctcgtgg aagtgtgtgg
     1621 caaagagggt cctcggcccg ggagcggcct tcccgtggag gaactcgagc acggacctcg
     1681 cgatggcata gccgtcgagg gtcccggtgc cctttccgat ctcgtccagg acgaccaggc
     1741 tccgctcggt catgctgttc aggatgttcg cgagctcgag catctccacc atgaaggtcg
     1801 actggccgct cgcgaggtcg tcgaaggccc cgacacgggt gaagacccga tccacgatcc
     1861 cgaccgtcgc atgggccgcg ggtacaaaag aaccagtctg ggcaaggatg gcgatgaggg
     1921 cgaccgccct catgtaggtc gacttccccg ccatgttcgc gccggtgatg atgaggatct
     1981 ggtccccgtt cccgtccatc agggtgtcgt tcgggacgaa acgccccccg aggacctgct
     2041 ccacgaccgg gtggcgcccc tcccggatcg cgagcttcag gctttcatcg aggaccgggc
     2101 gcgtgtattc gttcacccgg gccacctcgg cgagggcgga ggccacgtcc agccccgcga
     2161 ggcttcgggc gagggcctgg aaccggggaa tctccgcctg gagggatcgg acgagggaga
     2221 ggtagagctc cgactcgagg gcgaggatcc tttcgtcggc ggaggcgatc tggtctccct
     2281 tctcggcgag ctctccggta atgaaccgct ctccccccgc cgtcgtctgc ttccgtgcgt
     2341 agccgggggg gaccaggtgc aggttggcct tggtgacctc gatgtagtag ccgaagacct
     2401 ggttgtaccc gaccctgagg gaccggatcc cggtcctctc acgctccttc tgctggagct
     2461 ccacgaccca gtccttcacc gaacgggaga gggagcggag cccgtccagg tcgggggagt
     2521 atccctcccg gatgatgccc ccctgtctca ccgacgccgg cgggtcgtcc gagattcccc
     2581 gggtgatgag ggctgccgcg tcgtgcagat ccccgagccc ctccaggctc tcccgcaccg
     2641 gggcgggcag ggtggccgcc ttcccgagca gggcccggat tccggggacc accgcgagaa
     2701 gatccctgag ggcgaggagg tcccggggac ccgcgctccc ggtggcgatc ctccccgcga
     2761 tcctctctac gtcggcacac cgcgcgagaa gggtcctcag ctcccggcac aggaccgcat
     2821 cgcccatgaa gaactccacc gcatcgagcc gggcaccgat ggccccgacg gagataaggg
     2881 gcgcggccag gcgccggcgg agctccctcc tccccatggc ggtcatggtg aggtcaaggg
     2941 tggaaaagag ggttccctcc ttctcccggg aacggatgcc ctcgaagatc tccaggttcc
     3001 ggagggtgat ggcatcgata agcatggccc gggacgggac cctgaccgag aggcccgtca
     3061 cgtggccgag gggcgagtgc atggtttccg atgcatacct gagagcagca cctgccgccc
     3121 ggaccgcctc cggatacgga tcaaagccga acccggcaag ggtgtcgaca tggaaatggt
     3181 caaggagagc cctcgcggcg gtatcgggtc cgaaggcctc gtccctgaag acggtgagga
     3241 tgaccccttc ggcctcgagc atagccttca gtcgtacggg caggtccggg gggatcaccg
     3301 cctccgccgg ccggtaccgg acgatctcgg accggatgtc cccgaggccc tccgcctccc
     3361 cggcgaccga ggcaaggaac tcaccggtgg tcgtgtcgag gaaggcgagg ccggcccggt
     3421 ctcctttccc gtcggcccag acaccggcca ggtaccgtga tcccatgagc tcacccgtac
     3481 ccggagcgtc ggcagtcccc ggcgtgatca ccctcgtcac cgcacgccgg acgatacccc
     3541 gggagatcga ggagtcctcc acctggtcgc agaccgcgac ccggtagccc ttcgccacga
     3601 gcctcccgat gtagccctcg gcggcgtcgt gggggacccc tgcgaggggg atccggttcc
     3661 cggacctgtc ccgggatcgg gaggtgaggg ctatgtcgag ctcccgggag acgagctcgg
     3721 catctggccc gaaggtctcg tagaagtccc cgatatggaa gaagaggatg gccccggggt
     3781 tctgctcctt gaaggagcgg tactgctcca tggcgggggt gagcctctcc ccgtctcctt
     3841 ctcccgtgga attccccgcc cggcccattc catcagctat cggcccgcga tgaaaaaggg
     3901 ttaccgatcc cacccgaccg gaaagatggg gctccttcca cgtttcctca cgtgccctgg
     3961 cgccttcctt gtccgaaaga ccgcaggaaa gctactgaga aaggcatata tatgacaccc
     4021 gtggttctcc cttatataga gtgccgcgga gggaaggggg gtagactctc ggggtgtccg
     4081 gagaatccga aaggattcct gcgaagctcc cgtatcggcg ccgggacatg aacggcgttc
     4141 ctcatgagtt tttcgccctc gaagatctcc cctggaagct cgaggggatc gtctccggtt
     4201 tcgcactccc gggatcccga cgacctgacc ggatccggat ctcatcgcca gaaaatccct
     4261 catcatcccc gtcccctcaa ggaggaacgt atcatggtca cgaagtatgg ctacaacccc
     4321 agctggtttt accggaacac cgtttcgggc ctcgatccgg tgcagggctc atgcaacgat
     4381 tgttacctca ttgccgccct cggcgccgtc gcatggacat gcccgggaca gatccggatt
     4441 ccggacccga gcgggaatat cagcgtccat ttctgggaca ctggcaggct ggcctggtcg
     4501 aactggttca cggctgcaaa gaccgagagc ctcccggtgg acgagagcgg gaacctcttc
     4561 ggtgcccggt ccatggatgc caacgaaacc tggcccgccg tctacgagaa gctctacgcc
     4621 ctcttcaggg gagtctcccc ctcggacgga ggacccgaca tcgccgggaa catgcccgac
     4681 gggaatgctc tggagtgcct gtcggccctt accggctgga agacggtgtc gctcctgacg
     4741 agttcctaca gccccgatgc tctctggacc gcgctgaagc cccggacctc cggctcgaag
     4801 accatgttcc ccatggttgc ctggacctac ccgaatctcc ccgccctcca gcccggcacc
     4861 gccatcatgc ccaagcactc ctttccggtt ctcgggatct acaccgacgc cggaggttcc
     4921 aactacctcg ttctccggaa cccccgggga tccgtgaccc ggaattccgg gatcagtatg
     4981 ctcgactccg ggtcctggac cttcacagag accatcttct cggcccccgg cgtagccatg
     5041 gcctttcccc ggcccgttcc gaggaccctt tcctttaacc tcggccaggg gatctttgcc
     5101 ctggccatga aggaggttcc cgcgtatttc gaggggatcg ggtacgtcac ctgagtgagg
     5161 gatcgccgcc ggaggagaac ccaggagatc gctatcatga cagggtcggg aaagatgagg
     5221 aaaggccgga tggggtggtg cctcctggct gtcgccttcc tcctccccct catcctgctc
     5281 ccagcccttg ccgctgagtg caccatcacc ccggacgtcc agagtcccca ggcatacccc
     5341 gacatctcgg gcgacaccgt cgtctggctg gaccaggggt cgagtgatct ctatctgttc
     5401 agcctctcgg aagggaatga aacgcgcctc acctcagacg gaatgttgaa ggcgagcccc
     5461 cttatcagcg gccggcttgt cgtctggctg gaggggtaca acctgatcct cctgaacctc
     5521 acgacgggag gcacgagcac cctctcgacc gatgccttca ctgcagccat ctcgggggat
     5581 ctgatcgcct ttgtgaattc caccggcgtt tccctgtaca atgccacgag tggagactcc
     5641 tcggctgtcg ccggtgaacc ttacgatcag atccaggcag tcatatccgg gaacagggtt
     5701 atctgggtaa acgggactga ctcagggaac gtcaccgttg gtgtggacat cttcatgagg
     5761 gatctcacca cggataccga gaaccttgtc gccgagaccc tgtgtgccgc aaatccttta
     5821 gggatctgca tcatggaccc ccagatatcg ggtgagagaa ttgtatggtc cgataaccgg
     5881 gacaacccgt acacggatgt atatctcctt gacctttctg ccccgcttga aaggaggatc
     5941 gtccagactc cctcttccag tccgaaacct gctatcgatg gtgaccttgt cgcctgggtg
     6001 gacta
//
LOCUS       DATGDI010000182        11100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_135300, whole genome shotgun sequence.
ACCESSION   DATGDI010000182 DATGDI010000000
VERSION     DATGDI010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135300"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VKO45_06735"
     CDS             complement(<1..534)
                     /locus_tag="VKO45_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMA05607.1"
                     /translation="MSKILVVDDEAIITMQLEERLTALGYEVVGMAASGEESIEKARR
                     LKPDIVLMDIVMPGKMNGIEAAKQIYEEMGIPCVFVTSYADDAIIEKAKEARPYGYIV
                     KPFNELEIKAAIEVGLFRSATEPRPRREKGPARRGGPPGAGPAEEEGESQYLDLPGIK
                     TILLKDIFTDIILFLYTD"
     gene            complement(527..4225)
                     /locus_tag="VKO45_06740"
     CDS             complement(527..4225)
                     /locus_tag="VKO45_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMA05608.1"
                     /translation="MKIRTQLIIGLTVFALLLLAVSVLVATTADEVGRLDRQREAVNT
                     LAFEVGELGYLTNDYTLYLEPQQAARWREKYDEIEGSLKGLAPETPEQLAIVDNLKAS
                     LANVRLVFDDVSGTASSSASPSLAMVQLSWSRMTVQTQGMAFDARRLSDLLEAEATHL
                     RTENNLLILSLMGLFSGLLLTSYFLFYRRTLSSLSILQGGTEIIGAGNLGHTIAGTSD
                     DEVGDLAQAFNRMTASLRSVTASKADLEKEMEVRRLAEAALRESEERLRSLYETMTEG
                     FASHEVVYEDGKAVDYLITGVNPAFERITGIPRAGAVGARASELYQTGKPPFLEVYAR
                     VASGAGPEHFETFFPPMKQHFSVSAFSPGKGKFATVFTDITDRKKAEEKIAHLASFPE
                     LNPSPVLEVNPAGEVVYANPAASQALGRLGLGENPAAFLPGDFGFLLPRLMEGDVEEE
                     VAVGERAYLVTLTLNPSTRTTRIYARDITERKKAEEGLRESEEKFRVLAVNTPDHILV
                     QDRDLRYVQVINPQLGLTVEEMIGKTDYDILSKEDADHLTRIKRRVLETGTSDLMSIP
                     LADREGNTQYFEGQYVQKLDTAGRVDGILGYFRNVSDRRHAEQVLRETSQYLENLINY
                     ANAPIIVWDRELRITRFNHAFERLTGRTAGEVMGKSLEILFPPETMSDSMEHIARTMA
                     GERWETVEIPVVHEDGSIRTVLWNSAPLFEADGKTISSAIAQGQDITDRKQMEEELHH
                     TSQYLENLINYANAPIIVWDPEFRITRFNHAFEHLTGRTAGEVTGQRLEILFPPDTAA
                     PSMDIIMKAMAGERWEVVEIPILHRDGNVREVLWNSATLYEADGTTVSSAIAQGQDIT
                     EQKRMMEEIARKANELSEANAELSAEVSQRKKAEETAGRALSVLHAALESTADAMLVV
                     DRSGKITSRNENFTTMWNIPDSVLQTLDTRAAIDSMASQLNDPDGFSARMQEILDHPQ
                     RESYDMLELLDGRIIERYSKPQRIGTSTVGRVFSFRDVTDRKRGELQLIESLDEKEVL
                     LREIHHRVKNNLQLTTSLLDMTRMRTRDPEASGVLTDIMMKIQTMAQIHTRLYESKQF
                     DRINMDRQIRDQIQALTNIYSSRDREILVEIDSPEVYIPLDKAIPCALALNEILSNAY
                     KHAFKGRKKGTVKYLAEEEDGHMRFTITDNGVGLPKGFDLSKASTLGLKLVRTLVEQQ
                     LKGTLTMRRNKGTEVIVDLPVRKGEEHV"
     gene            complement(4231..5244)
                     /locus_tag="VKO45_06745"
     CDS             complement(4231..5244)
                     /locus_tag="VKO45_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA05609.1"
                     /translation="MNRILVALGLALVVGASATVGAWAFLDSQKNSVGHPDTVLVAWS
                     PFESTALFWIAEEMHFFERNGLNITLRRYDSGAGSLDGVMKGEADLAVGVSEFPLARK
                     ALEGARVRTLGNIDKGHFIYLVARKDRGIARVSDLKGKRIGTTVGTIADFHLGRFLML
                     HAIGMQDITLVDVRTPEGWVDAVARGDIDAIATAQPYAGKAMGRLGDNAVSWPIQSGQ
                     PLFANIISTESWIEAHPGSAARFLESLAEAEEYLNSHPAESMAIVQSRLDLDPGYMDS
                     VWQQNRFSLSLDQSLVLAMEDEARWMIANNLTDARTVPDFREVIEPRFLEEVRPGSVN
                     IIG"
     gene            5583..6500
                     /locus_tag="VKO45_06750"
     CDS             5583..6500
                     /locus_tag="VKO45_06750"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMA05610.1"
                     /translation="MDSVTHALAASSLFLAAGHPDLVPYAALGSVAMDIDILLMRFPE
                     RNPRYYVFTHGGFTHSIVGALLIGSLVYAATGMFTLAGPGAWPFGAGLGAGGLLALLA
                     GSLTHVFSDFLAYPGIPVLYPLTDRKYTAGIFAGPSLVLLVVSWTYLGTLLLSVTTLA
                     DYRTWAWIFVGYVGGKALLKVFLAATTDGATVPTKNPLKWFVIRDEDGSYSIQSRNLL
                     GGLTEPRIFPKFRNVSPAEVEQYRNLPEVRRHRYNSYITTVEKNGDTVTFRDPFRAEG
                     FTRYPFDNATVDVLSGTEVPGSGRSAKNR"
     gene            complement(6503..6772)
                     /locus_tag="VKO45_06755"
     CDS             complement(6503..6772)
                     /locus_tag="VKO45_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05611.1"
                     /translation="MALVLEITPAANLLFSALIVIVGLVAYGKTRKDIPIYIAGAFLL
                     FGLSHFATILGAGDAMTIPILAIRIIGYILILGVLYLGYAQEEGN"
     gene            6920..7417
                     /locus_tag="VKO45_06760"
     CDS             6920..7417
                     /locus_tag="VKO45_06760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA05612.1"
                     /translation="MPEEFDLTPIASSDREAVIDLFNYYIEHSFAAYPTERVPYEFFR
                     MVEEVMKGFPTVAARDRKGTLIGFAFLHPHNPMPTFAHTAEVTYFVKPECTGKGIGSR
                     MLAFLEEGGRKRGISCLLASISSLNEGSIRFHTRHGFVECGRFREIGRKKGRYFDTVW
                     MEKRI"
     gene            7481..7565
                     /locus_tag="VKO45_06765"
     tRNA            7481..7565
                     /locus_tag="VKO45_06765"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7517..7519,aa:Leu,seq:caa)
     gene            7722..8267
                     /locus_tag="VKO45_06770"
     CDS             7722..8267
                     /locus_tag="VKO45_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05613.1"
                     /translation="MEREGKYLVTLAILSAVLLTGIAAAQDGGLVLEKKGDIVTISGS
                     TNLAAGDLLQVNVVSAAFTPTEKGTGGGFSGTAGTAAVQQGSSLNSFSFDVNVSTFPP
                     GLYLVTVESVEPRLTESTRFVLPWTPVPAEITTLPTTLPVTTPRATTVAATASPTPAP
                     LPVILPLAAVALASFILLRRR"
     gene            8427..9527
                     /locus_tag="VKO45_06775"
     CDS             8427..9527
                     /locus_tag="VKO45_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA05614.1"
                     /translation="MKDDRYYNRVLDESITAVLHQAVKILASRPRLLSFAAGTFVSQK
                     RAASRRRKLSAQGIQVPAVMIASITRRCNLSCKGCYMKAQQREPGPEMTPDQLRTVVA
                     QADDLGISFLVLAGGEPLVRAGEILSLAREFPEMIFGVITNGLLIDKALAENLAARRN
                     IVPIVSFEGLEGETDTRRGAGVYRRLMETCSLLDRAGAFFGCSLTVTRRNHAQVTDED
                     FIQRMLGAGCRVFAFVEYVPVQEGTEGLVLSGEQRRSLVGCLREYPDRFPAIFLGFPG
                     DAETFGGCLSSGRGFLHVSATGDLEPCPAAPFSDANLTKMPLREALQSRFLGEIRRHH
                     DRLTETRGGCALWTNRGWVRSVLMGHVKDSPD"
     gene            complement(9545..10057)
                     /locus_tag="VKO45_06780"
     CDS             complement(9545..10057)
                     /locus_tag="VKO45_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05615.1"
                     /translation="MTLVTRGTLAAALALVLLGCLLAAPGCSATEKGPLFPPMTTPGP
                     ATPPPLPQTSPPLTPRSAATTPTPVDLSSPPKTLVRTPYPVPPIVGTWYAPPPDDLTF
                     EFRPDGYFVERSPNFRTYVGTWRISEEGEGDFYDADILDRWGYKKPAHLLYASGTLLT
                     KGIGTMHRVS"
     gene            complement(10125..10667)
                     /locus_tag="VKO45_06785"
     CDS             complement(10125..10667)
                     /locus_tag="VKO45_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HMA05616.1"
                     /translation="MAGLFLAIPAIILVARIVETSLETVRTVYITRGHTYLASSIGVG
                     KVAVWLLSTGLVLTNLTNIPSIVAYIAGYGIGTVAGMEIEDRISLGNVVVRIISPRDP
                     EPLVEHVCRLGYGITRLAGSGRSTSQVSVLLMVVPRKELGQLLAVLQRDYPDLLFTVE
                     DIRILHDHGPIYFGKGMKQG"
     gene            10868..>11100
                     /locus_tag="VKO45_06790"
     CDS             10868..>11100
                     /locus_tag="VKO45_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2121 domain-containing protein"
                     /protein_id="HMA05617.1"
                     /translation="MTLVMAFVGREGAVMAADMREITFIGDRESVERLEGELYDGRIV
                     TDGELKGRALELGVILSIRDDKVKAREREGVLVG"
BASE COUNT         1940 a         3532 c         3511 g         2117 t
ORIGIN      
        1 gtcggtgtac aggaacagga tgatgtcggt gaagatgtcc ttgaggagga tggtcttgat
       61 gccggggagg tccaggtact gggattctcc ctcctcctcc gcaggtcccg ccccgggagg
      121 cccccccctc cgggccggtc ctttctcccg cctcggcctc ggctcggtgg cactccggaa
      181 gagtccgacc tcgatggccg ccttgatctc gagctcgttg aagggcttca cgatgtagcc
      241 gtacggcctc gcctccttcg ccttctcgat gatggcgtca tcggcgtacg aggtgacgaa
      301 gacgcacggg atccccatct cctcatagat ctgcttcgcc gcctcgatcc cgttcatctt
      361 tcccggcatg acgatgtcca tgagcacgat gtccggtttc agccgccggg ccttctcgat
      421 gctctcctcc cccgatgcag ccatgcccac gacctcgtac ccgagggcgg tcagccgctc
      481 ctcgagctgc atcgtgatga tggcctcatc gtcaaccacg agtatcttag acatgctcct
      541 cccccttcct cacgggcaga tccacgatca cttcggtccc cttgtttctc ctcatggtga
      601 gggtgccctt cagctgctgc tccacgaggg tcctcaccag cttgagcccc agggtactcg
      661 ccttgctgag gtcgaacccc ttcggcagcc ccaccccgtt gtcggtgatg gtaaaccgca
      721 tatgcccgtc ctcctcctct gccaggtact ttactgtacc tttcttccgg cccttgaagg
      781 catgcttgta cgcattggag aggatctcgt ttaaggcgag ggcacagggg atggccttgt
      841 cgagagggat gtacacctcc ggagaatcga tctccacgag gatctcccgg tcccggctcg
      901 agtagatgtt cgtcagggcc tggatctggt cccggatctg ccggtccatg ttgatccggt
      961 caaactgctt gctctcgtag agccgggtgt ggatctgggc catggtctgg atcttcatca
     1021 tgatgtcggt gaggacaccg ctcgcctccg ggtcccttgt cctcatccgg gtcatgtcca
     1081 gcaggcttgt cgtgagctgg aggttgttct tcacccggtg atggatctcc cggaggagca
     1141 cctccttctc gtccagggac tcgatgagct ggagctcccc acgcttccgg tccgtgacgt
     1201 cccggaagct gaacaccctc ccgaccgtgg aggtcccgat cctctggggc ttcgagtacc
     1261 gctcgatgat cctgccatcg aggagctcca gcatgtcgta gctctccctc tggggatggt
     1321 cgaggatctc ctgcatccgc gccgagaacc cgtcgggatc attcagctgc gacgccatcg
     1381 aatcgattgc cgcccgggtg tccagggtct gcaggaccga atccgggatg ttccacatgg
     1441 tggtaaagtt ctcgttgcgg ctcgtgatct tccccgagcg atcgaccacg agcatggcgt
     1501 ccgcggtcga ttcgagggcc gcgtggagga ccgagagggc ccttcccgcc gtctcctcgg
     1561 ccttcttgcg ctgggagacc tctgccgaga gctcggcatt ggcttcggag agctcattgg
     1621 ccttccgggc gatctcctcc atcatcctct tctgctcggt gatgtcctgc ccctgggcta
     1681 tggccgaaga gaccgtggtc ccgtccgcct cgtagagggt cgcggagttc cagagcacct
     1741 ccctcacgtt cccgtcccgg tggaggatgg ggatctccac cacctcccac cgttcgccag
     1801 ccatggcctt catgatgatg tccatggacg gggcggccgt gtcaggggga aagaggatct
     1861 ccaggcgctg gcccgtgacc tccccggcgg tccgtcctgt caggtgctcg aaggcgtggt
     1921 tgaaccgggt aatccggaac tccgggtccc agacgatgat gggggcattt gcgtagttga
     1981 tcaggttttc caggtactga gaggtatggt ggagctcttc ctccatctgc ttccggtcgg
     2041 taatgtcctg cccctgggca atggccgagg atatcgtctt cccatcggcc tcgaacaggg
     2101 gggcggagtt ccagagcacc gtcctgatac ttccgtcctc gtggacgacg gggatctcca
     2161 cggtttccca gcgctcgccg gccatggtcc gggcgatgtg ttccatggaa tccgacatgg
     2221 tttcgggcgg gaagaggatc tcgaggctct tccccatcac ttcccccgcg gtccgcccgg
     2281 tcaggcgctc gaaggcgtgg ttgaaccggg tgatgcggag ttcccggtcc cagacgatga
     2341 tgggggcgtt cgcataattg atcaggttct ccaggtactg ggaggtctcc ctgagcacct
     2401 gctctgcgtg cctccggtct gacacgttcc ggaaataccc gaggatgccg tcaaccctgc
     2461 ccgccgtatc gagcttctgg acgtactggc cctcgaagta ctgggtgttc ccttccctgt
     2521 ccgcgagggg gatgctcatc aaatccgagg tgccggtctc gaggacccgc ctcttgatcc
     2581 gggtgaggtg atcggcatcc tccttcgaga ggatgtcata atcggtcttc ccgatcatct
     2641 cctccacggt gagccccagt tgcgggttga tgacctggac gtaccgcagg tcccggtcct
     2701 ggacaaggat atggtccggt gtgttgacgg cgaggaccct gaacttttcc tcgctctcgc
     2761 gcagaccctc ctcggccttc ttgcgctcgg tgatgtcccg ggcatagatc ctcgtggtcc
     2821 gggtagaggg gttcagggtc agggtcacga ggtatgccct ctcccccacg gccacctcct
     2881 cctcaacatc cccctccatg agcctcggga gcaggaaccc gaaatccccc ggcaggaagg
     2941 cggcgggatt ttccccgagc cccagcctcc cgagggcctg ggaggccgcc ggattggcat
     3001 agaccacctc ccccgccggg ttcacctcga ggacggggct cgggttcagc tcggggaagg
     3061 acgccaggtg ggcgatcttc tcctcggcct tcttccggtc ggtgatgtcg gtgaacaccg
     3121 tcgcaaattt ccccttcccg ggagagaagg ccgataccga gaagtgctgc ttcatcgggg
     3181 gaaagaacgt ctcgaagtgc tctggcccgg ccccggaggc caccctcgcg tacacctcca
     3241 ggaagggcgg tttccccgtc tggtacagct ccgaggccct cgcacccacc gcaccggccc
     3301 gggggatccc ggtgatccgt tcgaaggcag gattcacgcc ggtgatgagg taatcgacag
     3361 ccttcccgtc ctcgtacacg acttcatggc tcgcgaagcc ctcggtcatg gtctcgtaga
     3421 gggatcgcag ccgctcctcg ctctcgcgca gggctgcctc tgccagcctg cgtacctcca
     3481 tttccttctc gaggtccgcc ttcgaggcgg tgacggacct cagggaggcc gtcatgcggt
     3541 taaaggcctg cgcgagatcc ccgacctcgt cgtcggatgt cccggcgatg gtatggccga
     3601 ggtttcctgc cccgatgatc tccgtcccgc cctggaggat ggagagggag ctcagcgtgc
     3661 ggcgatagaa gaggaagtag ctcgtgagga ggagtccgga gaacaggccc atcagggaga
     3721 ggatgagaag gttgttctct gttctgagat gggtggcctc tgcctccagg aggtcagaga
     3781 gcctccgggc atcgaaggcc attccctggg tctggaccgt catcctgctc cacgagagct
     3841 ggaccatggc gagggagggg ctcgccgagg acgatgcggt ccccgagaca tcatcaaaga
     3901 cgaggcgcac gttcgcgagg ctagccttga ggttgtccac gatggccagc tgctcggggg
     3961 tttcgggggc aaggcctttc agggatccct cgatctcatc gtacttctcc cgccaccggg
     4021 cggcctgctg gggctcgagg tacagggtgt agtcattcgt gagataaccg agctccccga
     4081 cctcgaaggc cagggtgttc acggcctccc tctgccggtc gagcctgccc acctcgtcag
     4141 ccgtggtggc cacgaggacc gagacggcga ggaggagcag ggcaaagacg gtgaggccga
     4201 tgatgagctg tgtccggatc ttcacccgta tcacccgatg atgttcaccg atcccggcct
     4261 cacttcctcg aggaaccgcg gttcgatgac ctcccggaag tccggaaccg tccgtgcatc
     4321 cgtgaggttg tttgctatca tccaccgggc ctcgtcctcc atggcgagga cgagggactg
     4381 gtccagggag agggagaacc ggttctgctg ccagacgcta tccatgtatc cggggtcgag
     4441 atccagccga ctctgcacga tggccatgga ttcggcgggg tgggaattga ggtactcctc
     4501 tgcctccgca agggattcca ggaacctcgc ggcggatccg ggatgcgcct cgatccagct
     4561 ctcggtggag atgatattcg cgaacagggg ctggccgctc tggatcggcc aggaaacggc
     4621 gttgtccccc aggcgtccca tcgccttccc ggcatagggc tgggcggtcg cgatggcatc
     4681 gatgtccccg cgggcgaccg catccaccca tccctcggga gtcctgacat ccacgagggt
     4741 gatgtcctgc atcccgatgg cgtggagcat gaggaacctc ccgaggtgga agtcagcaat
     4801 ggtccccacg gttgtcccga tcctctttcc cttcaggtca gagaccctgg cgatcccccg
     4861 gtccttccgg gcgacgaggt agatgaaatg gcccttgtcg atatttccga gggtacgcac
     4921 cctcgctccc tcgagggcct tccgggcgag ggggaattcg ctcactccca ccgcaaggtc
     4981 cgcctcgccc ttcatcaccc cgtccaggga gcctgccccg gaatcgtatc tccggagggt
     5041 gatgttgagg ccgttcctct cgaagaaatg catctcttcg gcgatccaga agagggcggt
     5101 ggattcgaag ggtgaccatg ccacgaggac cgtatccgga tgtccgacgc tgtttttctg
     5161 ggagtcgagg aaagcccatg caccgaccgt ggccgatgca cccacgacaa gggcaaggcc
     5221 gagggcgacg aggattctgt tcatcgattc accatggaca atcgtccgtc atgccatcct
     5281 ctgccgcatc cggtcggggt gaaggtcacc ggccggcacc agctcgcgca gcagggaaaa
     5341 tcgtcgtatg acgagaaata ggaggagagg ttaaaataac ctttgcctga atctcaattt
     5401 ttgaaagccg agcgaaggcg aggccgagaa aatatcaatt ttcgaagccc tgcgccccgt
     5461 tccggcacag gccgcgactc ccggctccct tccgaaggtg gccgtgacgg gatccccgaa
     5521 ataatcaccc tttttacccc tcttcagtca cctatttccc catgagggca taacctgaga
     5581 tcgtggactc cgtcacccat gccctggccg catcctccct cttcctcgcc gcagggcatc
     5641 ccgaccttgt cccctacgcg gccctcggat ccgttgcgat ggacatcgac atcctcctca
     5701 tgcggttccc cgagaggaac ccgaggtact atgtcttcac ccacggggga ttcacccaca
     5761 gcatcgtcgg ggccctcctg atcgggagcc tcgtgtacgc cgcgaccggg atgttcaccc
     5821 tggccgggcc gggcgcctgg cccttcggcg ccgggctcgg cgcaggaggc ctcctcgccc
     5881 tcctcgcggg ttccctcacc catgtcttct ccgacttcct tgcctatccc gggataccgg
     5941 tcctctatcc cctgaccgac cggaagtaca ccgccggcat cttcgcgggg ccgagcctcg
     6001 ttctcctcgt ggtgagctgg acatacctcg ggaccctcct cctctcggtc acgaccctcg
     6061 ccgattaccg gacctgggcg tggatcttcg tcgggtacgt ggggggaaag gccctcctca
     6121 aggtcttcct cgccgcgacc accgacgggg ccaccgtccc cacgaagaat ccgctgaaat
     6181 ggttcgtgat ccgggacgag gacggctcgt actccatcca gtcccggaac ctcctcggcg
     6241 gcctgaccga gcccaggata ttcccgaagt tccggaatgt ctcacccgcc gaggtagagc
     6301 agtaccggaa cctgccggag gtcaggcgcc accgctacaa ttcatacatc acgacagtgg
     6361 agaagaacgg cgacacggtc accttccggg atcccttcag ggcagagggc tttacccggt
     6421 atccctttga taacgcaacg gtggacgtgc tctcggggac ggaggtgccg ggatcgggaa
     6481 gatccgcaaa aaaccgctga ccctaattcc cttcttcctg tgcatacccc aggtacagca
     6541 ccccgaggat caggatatat ccgatgatcc ggatcgcgag gatgggtatg gtcatggcat
     6601 ccccggcgcc caggatggtc gcgaagtggg agagcccgaa gaggaggaag gctccggcga
     6661 tgtagatggg gatatccttc cgggtctttc cgtacgcgac caggcccacg atgacgatga
     6721 gggccgagaa gaggaggttc gccgcgggtg ttatttcaag gacgagagcc acaggagatc
     6781 accacgtacc catgtaaccg ctcatcgggg ttccgaacga tgaaggttgc gaaacgatcc
     6841 tcccccatgc agggtgcaaa gaacctatat cgggaaagaa caacgggatg acaggaatcc
     6901 cgtttcccga ggagggacga tgcccgagga atttgacctc acccccatcg cgagttcgga
     6961 cagggaggcc gtcatcgacc tcttcaacta ctacatcgag cacagcttcg cggcctatcc
     7021 cacggagcgt gttccctacg agttcttccg gatggttgag gaggtcatga agggtttccc
     7081 gaccgtcgcc gcaagggacc ggaaggggac ccttatcggg tttgccttcc tgcatcccca
     7141 caaccccatg cccaccttcg cccacaccgc cgaggtgacg tacttcgtca aaccagagtg
     7201 cacgggaaag gggatcggtt cgaggatgct cgcctttctc gaggaagggg ggaggaaacg
     7261 ggggatctcc tgcctcctcg cgagcatctc ctccctcaac gagggaagca tccggttcca
     7321 cacccgccac ggctttgtcg agtgcgggag gttccgggag atcgggagga agaaggggcg
     7381 gtacttcgac accgtgtgga tggagaagag gatctgatcc gtgatttccg cccttccctc
     7441 cggataacct catatggaac ccgggggaaa tactccctga gcgagggtat ctaagcctgg
     7501 tcaaaagagg cagactcaag atctgctcca gaagtggttc gcgggttcaa atccctcccc
     7561 tcgcactctt ctccgggttt tccccggcgg gtactcgaat ctccgggacc tcccgaagcc
     7621 ttaatacgga tgaggaaaca tggacagaca atcccgccac gacgaagccg gcccgacccg
     7681 gtgaaactga attcttgccg gggtagcagg aggaagattc catggagaga gaagggaagt
     7741 acctggtgac cctcgccatc ctttccgccg tcctcctcac gggaatcgcg gcggcgcagg
     7801 acgggggcct cgtcctcgaa aagaaagggg acatcgtcac catctcgggg tccacgaacc
     7861 ttgccgcggg cgacctgctc caggtgaacg tggtctcggc agcctttaca ccgaccgaga
     7921 agggcaccgg agggggcttt tcgggcacag cggggacggc ggccgtccag cagggttcat
     7981 ccctgaacag cttcagcttc gacgtgaacg tctcgacctt cccgcccggg ttgtatctcg
     8041 tcaccgtgga gtccgtggag cccaggctca cggaatcgac ccggttcgtc ctgccctgga
     8101 ccccggttcc cgccgagatc accaccctgc cgacaaccct gcccgttacc acaccgaggg
     8161 caaccaccgt cgcggccacg gcatcaccga caccggcacc tctcccggtc atcctcccgc
     8221 tggcggccgt cgccctcgcg agcttcatcc tcctccgccg gcgctgattt tcggaaggac
     8281 tttcccgtcc cggggaccat gatgatggac gaagaatgcc ggaggaggaa gggaggcgat
     8341 ccctccgagg gaaagaagga gagggggatc cgggaataac cggtggcagg ctcctggtgc
     8401 agccttatcc tccccgctca ggcgaggtga aggacgaccg gtactacaac cgggttctcg
     8461 acgagagtat cacggcggtc ctgcaccagg cggtaaagat cctcgcctcc cgcccccggc
     8521 tcctctcctt tgcggcggga accttcgtct cccagaagag ggccgcatcc cggcggcgga
     8581 aactctcggc gcaggggatc caggtgccgg cggtgatgat cgcctccatc acccggcggt
     8641 gtaacctctc ctgcaaggga tgctacatga aggctcagca acgggagccc gggcccgaga
     8701 tgacccccga tcaactcagg accgtcgtcg cccaggctga cgatctcggg atatccttcc
     8761 ttgtcctcgc cgggggcgaa cccctggtgc gggccggcga gatcctctcc ctcgcccggg
     8821 agttcccgga gatgatcttc ggggtgatca cgaatggcct cctgatcgac aaggccctgg
     8881 cagagaacct tgcggccagg cggaacatcg tccccatcgt cagcttcgag ggactcgagg
     8941 gggagaccga cacccgccgg ggggcagggg tgtaccggcg gctgatggag acctgctccc
     9001 tcctcgaccg ggccggggcc ttcttcggat gctccctcac ggtcacccgg aggaaccatg
     9061 cccaggtcac cgatgaggat ttcatccaga ggatgctcgg ggccgggtgc cgggtcttcg
     9121 cctttgtcga gtacgtcccg gtgcaggagg ggacggaagg tctcgtcctc tccggggagc
     9181 agaggaggtc cctcgtcgga tgcctccgcg agtacccgga caggtttccc gccatcttcc
     9241 taggcttccc cggggacgcg gagaccttcg ggggctgcct ttcctcgggc aggggttttc
     9301 tccatgtcag tgccacggga gacctcgagc cctgccccgc cgcccccttc tccgacgcaa
     9361 acctcacgaa gatgcccctc agggaggccc tgcagtcccg gttcctcggg gagatccgga
     9421 ggcaccatga ccgcctgacc gagacccggg gagggtgtgc cctctggacg aaccgcgggt
     9481 gggtccgctc ggtcctcatg gggcacgtga aggactcccc cgactgacga ccttcttcag
     9541 aagattacga gacccggtgc atcgtcccga ttcccttcgt gagaagggtt ccggaggcgt
     9601 agaggaggtg cgccggcttc ttgtaccccc accggtcgag aatgtcggcg tcgtagaagt
     9661 ccccctctcc ctcctcggag atcctccagg tccccacgta ggtccggaag ttcggggacc
     9721 gctcgacgaa gtacccgtcg ggacggaact cgaaggtcag gtcatccggg ggaggggcgt
     9781 accatgtccc gacgatggga gggacggggt agggggtccg gaccagagtt ttcggggggc
     9841 tcgacaggtc gaccggagtc ggggtcgtgg ccgcagagcg gggggtgagg ggtgggcttg
     9901 tttgcgggag ggggggcggc gtcgcagggc ccggtgtcgt catgggagga aagaggggtc
     9961 ccttctccgt cgccgaacac cccggggcgg cgaggaggca tccgaggagg acgagggcga
    10021 gggcggcagc cagggttcca cgggtaacga gggtcattcc tgtgaaatcc tctctcccgg
    10081 ccctcgaaat actttccggg ggagattgag ggggcgggac caggtcagcc ctgcttcatc
    10141 cccttcccga agtagatggg gccatggtca tggaggatcc ggatgtcctc gacggtgaag
    10201 aggaggtcgg ggtagtccct ctggaggacc gcgaggagct ggccgagctc cttcctcggg
    10261 accaccatga ggagcaccga gacctgggac gtcgaccgtc ccgacccggc cagccgggtg
    10321 atcccgtacc cgagccggca gacatgttcc acgaggggct ccgggtcccg gggactgatg
    10381 atgcggacga cgacgttccc caggctgatc cggtcctcga tctccatacc ggcgacggtg
    10441 ccgatgccgt agcccgcgat gtacgcgacg atggacggga tgttcgtcag gttcgtgagg
    10501 acgaggccgg tggagaggag ccatacggcc accttgccga cgccgatgga ggaggcgagg
    10561 taggtgtggc cccgggtgat atacacggtc cttaccgtct caagggaggt ctcgacgatc
    10621 cgggcgacga ggatgatcgc gggaatggcg aggaaaagac cggccaaaag ctccttcaac
    10681 cccgtggatg aggagaactc tatggaatgc catatatagc gttttcctcc tcccgggaaa
    10741 ttgtatccgg tcgggagctt catcggggtc gatcggtatc tttctcaccc ggagggtcca
    10801 aaccggtgca gggcaccatg cagggatccc gtggatttct ccggggcccg gcggagggaa
    10861 tggccacatg accctcgtca tggccttcgt cggcagggaa ggcgccgtca tggccgccga
    10921 catgcgggag atcaccttca tcggcgaccg ggaatcggtg gaaagactcg aaggggagct
    10981 ctacgacggg aggatcgtca ccgacgggga gctcaagggg cgggccctgg agctcggggt
    11041 gatcctctcc atccgggatg acaaggtcaa ggcccgcgaa agggaggggg tgcttgtggg
//
LOCUS       DATGDI010000183        11356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_338668, whole genome shotgun sequence.
ACCESSION   DATGDI010000183 DATGDI010000000
VERSION     DATGDI010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 11356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11356
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338668"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VKO45_06795"
     CDS             <1..543
                     /locus_tag="VKO45_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMA05618.1"
                     /translation="ITGYRVGPGGAQGLYGIHPDLTTLGKILGGGLPIGAFGGRREIM
                     DYIAPCGPVYQAGTFSGNPLSLSAGTATIRYLRGHGDLYRRLDGMSRAIQDSLPRDAP
                     GSFVRLGSMFKFFFRRTPPRDYREAKESDTGAFAKFWDGMLARGIFLPPSQFETNFLS
                     AVHSDDDIEAIARGYRECLS"
     gene            531..1427
                     /gene="hemC"
                     /locus_tag="VKO45_06800"
     CDS             531..1427
                     /gene="hemC"
                     /locus_tag="VKO45_06800"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HMA05619.1"
                     /translation="MPLVIGTRGSKLALLQAERVCRLLAGQGVEVETVIVRTRGDEET
                     EVPLHEVGEQGLFVKALDDAILRGEIDCAVHSMKDIPAKRPGGLITAAILERDSPADF
                     FALSTAMKDVSIVGTSSMRRRAQILRHDSSVEVRPLRGNVDTRIRRLLAGDFHAIILA
                     EAGLDRLGYHLRGFRLPPERFVPTANQGAIAVVARDDPLLLITLSSLDHTPTRRDVNA
                     ERAVIEELGGGCFAPVGVYCRDGHLIAEVLSLDGQRTERIEEEVADADAARAAGRRLA
                     EKARDLLEAARCIPGGKDGAEG"
     gene            1411..2166
                     /gene="cobA"
                     /locus_tag="VKO45_06805"
     CDS             1411..2166
                     /gene="cobA"
                     /locus_tag="VKO45_06805"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HMA05620.1"
                     /translation="MAPRGKVFLVGAGPGSPDLLTVKAGRILGEAEVVLYDQLAGGVR
                     DLLPAGAELIDCGKSGSRHTLEQDEIERIMVARALEGKRVVRLKGGDPFLFGRGGEEL
                     EALRDAGIPVELVPGVPSAVAVPGCVGIPVTHRGYASEVVILTGHEEPGKEESGIDWN
                     LLAGFRGTIVILMGVKNLPSIAGELIARGRDPATPVAIIERGLAPGQRVTTGPLGEIG
                     EIMRRAGVRPPAVIVIGEVVGLYRGEELCEARK"
     gene            2148..3791
                     /gene="ilvD"
                     /locus_tag="VKO45_06810"
     CDS             2148..3791
                     /gene="ilvD"
                     /locus_tag="VKO45_06810"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HMA05621.1"
                     /translation="MRSEEVKKGYIRAPNRALLRSLGVTEGEMDRPFIGIADAWNTIV
                     PGHVHLRQLSAKVAEGIAAAGGVPFEFGVIGICDGIAMGHEGMRYSLPSRENIADSIE
                     LMVEAHRFDGLVGVGTCDKIVPGMLMAAVRCDVPSILVTGGPMLPGSLGGKDLSLIDV
                     FEAVGRVAAGTMDEGELAALECAAMPGCGSCQGLYTANTMACVTEALGMSLAGCATIP
                     AVDAGKLRIARQSGERVLDLVREKVTPRGIVTPESIRNAIRVDMALGGSTNTILHLMA
                     LATEAGIPLDLDTFNEIGAGIAHICDMQPAGPHSMLALHRAGGIPAVLSRLKEVLEDA
                     PTVTGPGIREIAAMARVTDPKIIHTLRQPIHAAGGLRVLRGTLAPDGAVVKAAAVEKD
                     MWRHRGPARVFDGEQEAMRAILARRIREGDVVVIRGEGPRGGPGMPEMLGPTSAIHGL
                     GYKRVALVTDGRFSGGTRGPCIGHVAPEAVAGGPIALIREKDIIEIDIGKMRLDLAVP
                     EKELAKRRRSWRPKERALTGVLARYAATVGQANLGAVQR"
     gene            3949..4536
                     /locus_tag="VKO45_06815"
     CDS             3949..4536
                     /locus_tag="VKO45_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HMA05622.1"
                     /translation="MVMIDKLLEGNHLFVTGEFAKKKAFYTGLARGQSPKTLWIGCVD
                     SRVTPERITSAEAGELFVHRNIGNIVPHASWTFATVLEYAIRHLKVEEIVICGHSDCG
                     AMKALGKASGDTYIPLWLNNAGDVLPKVEADIAKLPRAPDEKGRRRMIEMENVRLQIT
                     HLRTYPLVKEAEAEGRIRVYGLYYDLESGRVSRVP"
     gene            complement(4497..4877)
                     /locus_tag="VKO45_06820"
     CDS             complement(4497..4877)
                     /locus_tag="VKO45_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05623.1"
                     /translation="MDDSVTIEIIGFSDAACGPFPCDPDRTCGLVECFPSGSFLAAVD
                     GLRHALASRFGDRVTLTVTLLDDGVPDRVKAIVERYHPPLPFVLLNGRVTPIGRISFT
                     RIVREIEAAPPGHGTRETRPDSRS"
     gene            5194..6210
                     /locus_tag="VKO45_06825"
     CDS             5194..6210
                     /locus_tag="VKO45_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMA05624.1"
                     /translation="MTPSSQKGEAERISRHGEALAEQGKYEEAVAEFDRALALAPDDG
                     EIWFRKGLVKKTMGLLDDALLAFDRAIWYQPREALVWFQKGIVLGLMGRIEEALRCFD
                     TILELDPSYAIRAWHEKGVNLGKLGRYEEALQSWDRIIEKDPKYVMAWYSKGIILSYL
                     GRHEEALRAFDKGLDIYPRSPDLWYQKGMIFTRTGNTERAQKCFEMALSINPRHEGSN
                     LEMGMILEMKGNHERAFLYYDRVIGMDPENAKAWYQKGSTLQRLGRNKEALDCYEKAL
                     GLDPMNMYIWFSKALLLVSMGQVTEAVQALDGALAIQEVPEVRRLRDALLSEGDEGET
                     PAGP"
     gene            complement(6289..7359)
                     /locus_tag="VKO45_06830"
     CDS             complement(6289..7359)
                     /locus_tag="VKO45_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05625.1"
                     /translation="MSLRPFLPLLAILFLLSLILGVQATEIRITTDPADQTTPSIWES
                     RIVWTDARDKTLNVYLYDVGTGTETRLARGTKDRKNPAIWENLVAMEDMAQGSPNILA
                     YDLLSQSGRPIMTASANEKFPRVAGNFIVWETSRSGDNDIALYNYTGAREEKLPPSTG
                     SRRSPAIWGNRMVWADTSSGASAIRLYDITTKAESPLTKGTSSQFNPWIYGNRVVWQD
                     SRNGNADIYLYDTSTGIETRVTTDPADQRNPRIWGDRIIWEDYRNGNPDIYMYTISTG
                     KEQALTKDQSVQRSPAIWGDRIVWEDYRNGNADIYMYEIPAVPGPSSPASGTQGQALP
                     MPGILPVIGAALFLAIAGRRAR"
     gene            7523..8062
                     /locus_tag="VKO45_06835"
     CDS             7523..8062
                     /locus_tag="VKO45_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05626.1"
                     /translation="MKPPSALILLLVLASVLMLAGCTQPVLNSSGQPSRSATPTPTPG
                     PVVAAEPYPPAEPYEIDPKFVRNDLRYRVDPGLFTEVYNGSYTLKWSSIGLLATVEEA
                     PMVLDFSVTPGSRDPVYSFFILTVRDAGSGVVVGQEGYGRTFSTENPKRLVFTSPGKY
                     HINLYGSLVDVDLAVSLKK"
     gene            complement(8069..8153)
                     /locus_tag="VKO45_06840"
     tRNA            complement(8069..8153)
                     /locus_tag="VKO45_06840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8115..8117),aa:Leu,seq:gag)
     gene            8301..8801
                     /locus_tag="VKO45_06845"
     CDS             8301..8801
                     /locus_tag="VKO45_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator of amino acid metabolism, contains ACT
                     domain protein"
                     /protein_id="HMA05627.1"
                     /translation="MWSVIMKEFADSPAQARVARFILENGFGVSDGGKVVANGVEIPA
                     THVAAAIGADRRVVDATARRILSVPALAPVFRSMRATPDLSRVAEPLGLSAFTVLPKN
                     AQEKGIVSAVIRVVSARNLTLRQIFVTDPYFSEEPKLVVIIEEKVPRGLFEEIRALPQ
                     VKQVII"
     gene            complement(8794..9435)
                     /locus_tag="VKO45_06850"
     CDS             complement(8794..9435)
                     /locus_tag="VKO45_06850"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HMA05628.1"
                     /translation="MNEDPEGISRRENHRAERGGDPEGISPGESQQGEALAVNAKGGS
                     PRENQQGEALVVRWEQLLIRAGCDRSVIAHARAVTALAFLFTESPVVNRDLVRTGAML
                     HDIGRGVTHGVDHAQRGAALATSLGVPPPVVSIIERHIGAGMTADECSLEGLLPRDCV
                     PRTLEEKIVANADNLVQGDRAGSIEATLEASIRLKKRIRRRIFRLWLEMESLR"
     gene            complement(9428..9946)
                     /locus_tag="VKO45_06855"
     CDS             complement(9428..9946)
                     /locus_tag="VKO45_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HMA05629.1"
                     /translation="MMSIAEILEDEAVRAYLNRLIGEEGLDLLERFPEGGEYSDEELA
                     EKTGINLNSVRHTLYTLYEKRLAEYRRLKNTETGWLTYLWTLRLDRIQAAVAEDLKMM
                     LEKLEARESFEEENDFYLCKTCGTFTFNEVVELDFCCPRCSQPVEHFDNEALLSALKR
                     RISAIRECLGYE"
     gene            complement(9943..11019)
                     /locus_tag="VKO45_06860"
     CDS             complement(9943..11019)
                     /locus_tag="VKO45_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HMA05630.1"
                     /translation="MNATVLIAGFATRHVAQSASKAGYTVCAVDHFCDQDLSWYTEDR
                     LKFEELDELPDRIAEMAGRHRIDFFIPTSGAESIGTPIPLAGTDRRTAARLLDKIEVQ
                     RFFEGKGIPAPPLAGEKDFPYLIKPRSGAGGWRNTIIRSPEDLSRWRRWSEEPGEILQ
                     KQVLGIPASVSCLADGRRAVALAANEQLLRGGDEAPFGFTGSVTPLLHPLAGRMKALA
                     VEAAAASGCVGSVGVDFVLGEEAVAIELNPRFQATVDTVEGSLGVSLFALHMDACRGI
                     LPQPVPEPRRCVARRILFAERDLVVTGDIAGMAPLVADIPWPGTAFEEGQAVVSVFGT
                     GPDRAGALRNLDNTLNKLERYMGR"
     gene            complement(11016..>11356)
                     /locus_tag="VKO45_06865"
     CDS             complement(11016..>11356)
                     /locus_tag="VKO45_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844362.1"
                     /note="catalyzes the S-adenosyl-methionine-dependent
                     2'-O-ribose methylation of C56 in tRNA transcripts;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HMA05631.1"
                     /translation="EDGVSWKGCIRSWKEKGGTVVHLTMYGIPLTETIGEIRMAEEIL
                     VVVGAEKVPGEIYGLADYNVSVTGQPHSEVSGVAVFLDHLFEGSTLGRTYPDAELRVY
                     PSRNGKRTGP"
BASE COUNT         1977 a         3661 c         3762 g         1956 t
ORIGIN      
        1 atcaccggct accgggtcgg ccccggcggg gcccaggggc tgtacggcat ccaccccgat
       61 ctcacgaccc tgggaaagat cctcggcggg gggctcccca tcggcgcctt cggggggcgg
      121 agggagatca tggattacat cgccccttgc ggcccggtgt accaggccgg gaccttcagc
      181 gggaatcccc tctccctctc cgcggggacc gctaccatac ggtacctccg tgggcatggc
      241 gacctgtacc gcaggctcga cgggatgtcc cgggccatcc aggattccct gccgagggat
      301 gcgcccggat ccttcgtccg gctcggctcg atgttcaagt tcttcttccg ccggacccct
      361 ccccgggatt accgggaggc gaaggagagc gacaccggtg ccttcgcaaa gttctgggac
      421 gggatgctgg ccagggggat cttccttccg ccctcccagt tcgagacgaa cttcctctcg
      481 gcggttcatt ccgatgatga catcgaggcc atcgcccggg ggtaccgcga atgcctctcg
      541 tgatcgggac ccgggggagc aagctcgccc tcctccaggc cgagcgggtg tgccggctcc
      601 tcgcaggcca gggggtcgag gtggagacgg tcatcgtccg gacccgcggc gacgaagaaa
      661 cggaggtccc cctccacgag gtcggggagc agggcctctt cgtgaaggcc ctggacgatg
      721 ccatcctccg gggcgagatc gactgcgcgg tccacagcat gaaggacata cctgcgaagc
      781 ggcccggtgg gctcatcacc gccgccatcc tcgaacggga ctccccggcg gactttttcg
      841 ccctttccac ggccatgaag gacgtgagca tcgtggggac ctcgagcatg cggaggcggg
      901 cccagatcct ccggcacgac agctccgtgg aggtgaggcc cctccggggg aacgtggaca
      961 caaggatccg gcggctcctg gccggggatt tccacgccat catccttgca gaggcagggc
     1021 tcgaccggct ggggtatcac ctccgggggt tccggttgcc cccggagcgg ttcgttccca
     1081 ccgcgaacca gggggccatc gcggtggtgg cccgggatga ccccctcctc ctcatcaccc
     1141 tctcctccct ggaccatacc ccgacgaggc gggatgttaa cgccgagcgg gccgtcatcg
     1201 aggaactcgg cggagggtgc ttcgcgcccg tcggggtcta ctgcagggac gggcacctca
     1261 tcgccgaggt cctctccctg gacgggcaga ggaccgagag gatcgaggag gaggtcgccg
     1321 atgccgatgc cgcgcgggcg gcgggaagga ggcttgcgga aaaggcacgc gaccttctcg
     1381 aggcggcccg ctgcattccc ggggggaagg atggcgccga ggggtaaggt cttcctggtg
     1441 ggtgccgggc ccgggagccc ggatctcctg acggtgaagg cgggaagaat cctcggggag
     1501 gccgaggtgg tcctctatga ccagctggcg ggaggcgtcc gggaccttct tccggcgggg
     1561 gccgagctca tcgactgcgg gaagtcgggc tcgcggcaca ccctcgagca ggacgagatc
     1621 gagcggatca tggtggcgag ggccctcgag ggaaagaggg tcgtccggct gaaggggggc
     1681 gacccgttcc tcttcggcag gggaggggag gagctggagg ccctccggga cgcggggatc
     1741 cccgtggagc tcgtgcccgg ggtcccgagc gccgtcgccg tccccgggtg cgtgggcatc
     1801 cccgtgaccc accggggata tgcctccgag gtcgtcatcc tcacaggcca cgaggagccc
     1861 gggaaggagg agtccggcat cgactggaac ctcctggccg ggttccgggg gaccatcgtg
     1921 atcctcatgg gggtgaagaa cctcccttcc atcgcggggg agctcatcgc ccgtggccgg
     1981 gacccggcaa ccccggtcgc catcatcgag aggggccttg caccgggcca gagggtcacc
     2041 acggggcccc tcggcgagat cggggagatc atgaggaggg cgggggtaag gccccccgcg
     2101 gtgatcgtca tcggggaggt cgtcggcctc taccggggtg aggagttatg cgaagcgagg
     2161 aagtgaaaaa gggatatatc cgggcgccca accgcgccct gctgcgctcc ctcggggtca
     2221 ccgaggggga gatggacagg cccttcatcg ggatcgccga cgcctggaac accatcgttc
     2281 ccggccacgt gcacctgcgg cagctgtccg caaaggtcgc ggaggggatc gccgcggcgg
     2341 gcggggtgcc cttcgagttc ggggtcatcg gcatctgcga cgggatcgcc atggggcacg
     2401 aggggatgcg gtactcgctt ccctcccggg agaacatcgc cgactccatc gagctcatgg
     2461 tggaggccca ccgtttcgac ggcctcgtgg gcgtcggcac ctgcgacaag atcgttcccg
     2521 ggatgctcat ggcggcggtg cgctgcgatg tcccgtccat cctcgtcacc ggcgggccga
     2581 tgctccccgg ctccctcggc ggaaaggacc tctccctcat cgacgtcttc gaggcggtgg
     2641 ggagggtcgc cgccgggacc atggatgagg gggagctcgc cgccctggag tgtgcggcga
     2701 tgcccggctg cggctcctgc cagggactct acacggcaaa taccatggcc tgtgtcaccg
     2761 aggctctcgg catgtccctt gccggctgcg cgacaatccc cgcggtggac gccgggaaac
     2821 tccggatcgc gaggcagagc ggggagcggg tcctggatct cgtgagggaa aaggtcaccc
     2881 cccgcggaat cgtcactccg gagagcatcc ggaatgccat ccgggtcgac atggccctcg
     2941 ggggatccac gaacaccatc ctccacctca tggccctcgc cacggaggcg ggcattcccc
     3001 tggatctcga cacctttaac gaaatcgggg ccgggatcgc ccatatctgc gacatgcagc
     3061 ccgccggtcc ccattccatg ctcgccctcc accgggcggg aggcattccg gccgtcctgt
     3121 cccggctcaa ggaggtactc gaggacgccc cgaccgtgac aggccctgga atccgggaga
     3181 tcgcggcgat ggcacgggtc accgatccga agatcatcca tacccttcgc cagccgatac
     3241 acgcggcggg cggcctccgg gtgctccggg gcacgctcgc tccagacggg gccgtcgtaa
     3301 aggccgccgc cgtggaaaag gatatgtggc gccaccgggg ccccgcccgg gtcttcgacg
     3361 gtgagcagga ggccatgagg gcgattctcg cgaggagaat cagggaaggg gacgtggtgg
     3421 ttatccgggg tgaagggccc aggggtggcc ccgggatgcc ggagatgctc ggcccgacct
     3481 cggccatcca cgggctcggg tacaagcggg tggccctcgt caccgacggc agattctccg
     3541 gggggacgag ggggccctgc atcggtcatg ttgcccccga ggcggtggcc ggcgggccca
     3601 tcgctcttat ccgggagaag gacatcatcg agatcgatat cgggaagatg agactcgatc
     3661 tcgcggtgcc ggagaaggag ctcgcaaagc gtcgccgatc ctggaggccg aaagagaggg
     3721 ctctcaccgg tgtcctcgcc cggtatgcgg caaccgtcgg ccaggcaaac ctcggagcag
     3781 tccagcggta ggctgtattc tcccaccggc cacccggtct ccaccctgtt ttcctcccac
     3841 atcctcatcg tttctccgct ccgtgaactc atgtcgtatc ctttatctgt cacgggagag
     3901 agttgagggt aaggctctgc cggcccggac cggcatcgcc tttctgtgat ggtcatgatc
     3961 gataagctcc ttgaaggcaa ccacctgttc gtcacgggtg agtttgcgaa gaagaaggca
     4021 ttttacacgg gtcttgccag gggccagagc ccgaagaccc tctggatcgg gtgcgtggat
     4081 tcgcgggtga cgcccgagcg gatcacctcc gccgaggcag gcgagctctt cgtccaccgg
     4141 aacatcggga acatcgtacc ccacgcgtca tggacctttg ccaccgtcct cgagtacgcg
     4201 atccgccacc tgaaggtcga ggaaatcgtg atctgcggcc actccgattg cggggccatg
     4261 aaggccctgg ggaaggcctc cggcgatacg tatattcccc tctggctgaa caacgcgggg
     4321 gatgtgctcc cgaaagtgga ggccgacatc gcgaaactgc cccgggcccc cgatgaaaag
     4381 ggccggcggc gcatgatcga gatggagaac gtcaggctcc agatcaccca tctgagaacc
     4441 tatccgctcg tgaaggaggc ggaggcagag gggaggatcc gggtgtacgg gctctactac
     4501 gaccttgagt ccggccgggt ctcacgcgtt ccgtgacccg gcggggctgc ctcgatctcc
     4561 cgcacgatcc gggtgaagga gatcctcccg atgggggtca ccctcccgtt gaggaggacg
     4621 aaggggaggg gtgggtggta cctctcgacg atggccttca cccggtcggg caccccgtca
     4681 tcgaggaggg tcacggtcag ggtgacccgg tcgccgaacc tcgaagcgag ggcatgccga
     4741 agcccgtcga cggccgcgag gaaggagccc gaggggaagc attcgacgag gccgcaggtc
     4801 cggtccggat cacagggaaa gggcccgcac gcggcatccg agaagccgat gatctcgatg
     4861 gtaacggaat cgtccatact ccctgtagaa tggatcctgc gtgaggaaaa agctatcaag
     4921 acctggcggg attgaaggcg ggaacagggc aggaggggcc ggcccgctga acaaaccccc
     4981 tgcaacccgg ttcacggaat ttccctctcg tgttcaaccc cgaaccggtt ctccggggcg
     5041 gagcatcccc ttccggatca agaccgatca tcggggagca caccaccggg ggtaggttta
     5101 ttaccgactc ggatatcatg ttggtatgat gcccggctgc ccctcccgtc ccgcgaggcc
     5161 gatgctgaga cgagagggga gtgcgggaag gccatgacac catcgtccca gaagggtgag
     5221 gcagaaagaa tttcccggca cggggaggcc ctggcagagc aggggaaata cgaagaggcc
     5281 gtggcggagt tcgaccgggc cctcgccctt gctcccgatg acggggagat ctggttcagg
     5341 aaggggctcg tgaaaaagac gatgggtctt ctcgacgacg ccctcctcgc ctttgaccgg
     5401 gcgatctggt accagccccg ggaggccctc gtctggttcc agaaggggat cgtcctcggc
     5461 ctgatgggga ggatcgagga ggccctcagg tgcttcgata ccatcctcga gctggacccg
     5521 agctacgcga tccgggcctg gcatgagaaa ggggtgaacc tcgggaagct gggccggtac
     5581 gaggaggccc tccagtcctg ggaccggatc atcgagaagg acccgaagta cgtgatggca
     5641 tggtacagca agggaatcat cctctcctac ctgggccggc acgaggaggc actccgtgcc
     5701 tttgacaaag gactcgacat ctacccgcgg agcccggatc tctggtacca gaaggggatg
     5761 atcttcaccc ggacgggaaa caccgagcgg gcccagaagt gcttcgagat ggccctctcc
     5821 atcaatccca ggcacgaggg atccaacctc gagatgggga tgatcctcga aatgaagggg
     5881 aaccatgaac gggccttcct gtactacgac cgggtcatcg gcatggaccc cgagaacgcg
     5941 aaggcctggt accagaaggg gtcgaccctc cagcgactcg gcaggaacaa agaggccctc
     6001 gactgctatg agaaggccct tggcctcgac cccatgaata tgtacatctg gttctccaag
     6061 gcgctcctcc tcgtgtccat gggtcaggtc accgaggcgg tccaggccct ggacggcgcc
     6121 ctggccatcc aggaggtccc cgaagtgcgg aggctccggg acgccctcct ttccgagggc
     6181 gatgagggag aaactcccgc cggaccatga tgggagcccg ggctcccctc gccgggaaga
     6241 gtggcagaca aaagggagag gagaggaggg ttcttcaccg gaagatcgtc agcgggctct
     6301 ccttccggcg atagccagga agagggccgc acctatcacg ggcaggatcc caggcatggg
     6361 caaggcctgg ccttgtgttc ccgatgccgg ggaggagggg ccgggaacgg caggtatctc
     6421 gtacatgtag atatccgcat tgccgttccg gtaatcctcc catacgatcc ggtcgcccca
     6481 gatcgccggt gagcgctgga cggactggtc tttcgtcagg gcctgctcct tcccggtcga
     6541 gatggtgtac atgtagatat ccgggttccc gttccggtaa tcctcccata tgatcctgtc
     6601 gccccagata cgcgggtttc gctggtccgc gggatccgtg gtgacccgcg tctcgatccc
     6661 ggtcgaggta tcgtagaggt agatatcggc gttcccattc cggctgtcct gccagaccac
     6721 ccggttcccg tagatccagg gattgaactg gctcgaggta cccttggtca gcggggattc
     6781 cgcttttgtc gtgatgtcgt agagccggat ggccgaggcc ccgctgctcg tgtccgccca
     6841 gaccatccgg ttgccccaga tcgcgggcga ccgccgggac ccggtactcg gggggagctt
     6901 ctcctccctt gcacccgtgt agttgtagag ggcgatgtcg ttatccccgc tccgggaggt
     6961 ctcccagacg ataaagttcc ccgcgacccg ggggaacttc tcgttcgcgg aggccgtcat
     7021 gatggggcgc cccgactggg agaggaggtc ataggcgaga atgttcgggc tcccctgtgc
     7081 catgtcttcc atggcgacaa ggttctccca gatggcagga tttttccggt cctttgtacc
     7141 cctcgcgagc cgggtctcgg tacctgtccc cacgtcatag agatagacat tcagggtctt
     7201 atccctggca tcggtccaga cgatcctgct ctcccagatc gagggagtgg tctggtcggc
     7261 cggatcggtg gtgatccgga tctcagtggc ctggacaccc aggatcaggg aaagaaggaa
     7321 caggatggcc aggagaggga gaaaggggcg aagtgacatg tgcaacaatt cctttgtgaa
     7381 tatatataaa agaatccttt tttatttgcc agatatccct tgagatcggt attttccggg
     7441 caatttccag cgatacaatg tccgggcaga tacccttgat acgagcactt ttttataaaa
     7501 agagggagat agatcctttc gtatgaagcc gccctccgcc ctcattctcc tccttgttct
     7561 cgcatcggtc ctgatgctcg cgggatgcac gcagcccgtc ctgaacagca gcgggcaacc
     7621 ctcccgttcc gcgaccccca cccccacccc cggacccgtg gtggcggctg agccctatcc
     7681 gccggccgaa ccctatgaga tcgatcccaa gttcgtgagg aacgatctcc ggtacagggt
     7741 cgatccgggt ctcttcaccg aggtatacaa cggctcgtac accctgaaat ggagctccat
     7801 cggcctcctc gccaccgtcg aagaggcccc catggtcctc gacttctcgg taaccccggg
     7861 cagccgggat ccggtgtaca gcttctttat cctcacggtc cgggacgccg ggtcgggggt
     7921 ggtggtcggg caggaagggt acggcaggac cttctcgacc gagaacccga agcggctggt
     7981 cttcacgagc cccgggaagt accacatcaa cctgtacggg agccttgtgg acgtggacct
     8041 ggccgtaagc ctgaagaaat aaaacggatg cgaggggagg gatttgaacc cacgaactcc
     8101 ttcgagaccg gcccctcaag ccggcgcctt agaccaagct tggcaaccct cgcgcgtaaa
     8161 taactacacc cggggattac aagaatcttc tccttcagcc accgggggcg aatccccggg
     8221 atcgccgccg gatctccggc catcatcgtg attctttccg gacacggatg tatataaata
     8281 tacaacgccc attctctctc atgtggtccg tcatcatgaa ggagttcgcc gattccccgg
     8341 cccaggcccg ggtcgcacgg ttcatcctgg agaacggctt cggcgtgagc gacggcggga
     8401 aggtggtggc gaacggcgtg gagatccccg ccacccacgt cgccgcggcc atcggggcgg
     8461 atcgacgggt ggtggacgcc acggcccgga ggatcctctc tgttccggcc cttgcgcccg
     8521 tctttcggag catgagggcg acccccgatc tctcccgggt ggccgagccc ctcggtctct
     8581 cggccttcac cgtcctcccg aagaatgccc aggaaaaggg gatcgtgagc gcggtcatca
     8641 gggtggtctc ggcccgcaac ctcaccctcc gccagatctt cgtcaccgat ccctacttct
     8701 cggaagagcc gaagctcgtg gtgatcatcg aggagaaggt cccccggggg ctcttcgagg
     8761 agatccgtgc ccttccgcag gtgaaacagg tcatcatctg aggctttcca tctcgagcca
     8821 gagccggaag atccgccgcc ggatcctctt cttcagccgg atgcttgcct ccagggttgc
     8881 ctcgatggac cccgcccggt ccccctggac caggttgtcg gcgttcgcca cgatcttttc
     8941 ctcgagggtc cggggcacgc agtccctcgg gaggagccct tcaagggagc actcgtccgc
     9001 ggtcatcccg gcgccgatgt gcctctcgat gatggatacg acgggagggg gaactccgag
     9061 ggaggtggcg agggccgctc cccgctgggc atgatcgacc ccgtgagtca cgccccgccc
     9121 gatgtcatga agcatcgccc cggtccggac gaggtcccgg ttcacgacag ggctttccgt
     9181 aaaaaggaag gcgagggccg tgacggcccg ggcatgggcg atgaccgatc tgtcgcagcc
     9241 tgcccggatg aggagctgct cccacctcac caccagggct tcaccctgtt ggttctctct
     9301 cgggctgccg cccttcgcgt tcaccgccag tgcctcgccc tgttggctct cccccgggct
     9361 gatgccctcg gggtcaccac ctcgctccgc tcggtggttc tccctccggc tgatgccctc
     9421 ggggtcctca ttcatacccg aggcattccc ggatggcgga gatccggcgc ttcagggcgg
     9481 agaggagggc ctcgttgtcg aagtgctcga cgggctggga gcaccggggg cagcagaaat
     9541 cgagctccac gacctcgttg aaggtgaagg tcccgcaggt cttgcagagg tagaagtcgt
     9601 tctcctcctc gaagctctcc cgggcctcga gcttctcgag catcatcttc aggtcctcgg
     9661 cgacggcggc ctggatccgg tcgaggcgga gggtccagag gtacgtgagc caccccgtct
     9721 cggtgttctt gagcctccgg tactcggcga gcctcttctc gtagagggtg tacagggtgt
     9781 gccggaccga gttcaggttg atgcccgtct tttcggccag ctcctcgtcg ctgtactccc
     9841 cgccctccgg aaacctctcc aggagatcga gcccctcctc gccgatgagg cggttcagat
     9901 aggcccggac cgcctcgtcc tcaaggatct ccgcgatgga catcacctcc ccatatatcg
     9961 ctccagcttg ttaagggtgt tgtcaaggtt tcggagggcc ccggcccggt ccgggcccgt
    10021 cccgaacacg ctcaccaccg cctgcccctc ctcgaaggcc gtgcccggcc agggaatgtc
    10081 ggccacgaga ggagccatcc cggcgatatc ccccgtcacc accaggtccc gttccgcaaa
    10141 gagaatcctc cgggccacgc agcggcgggg ttccggaacc ggttgcggga ggatcccccg
    10201 gcaggcgtcc atgtggaggg cgaagaggga aacccccagg gatccctcga cggtgtcgac
    10261 ggtggcctgg aaccgggggt tcagctcgat ggccaccgcc tcctccccga ggacgaagtc
    10321 gacccctacg gagcccacgc aaccgctcgc cgcggccgcc tccacggcaa gggccttcat
    10381 ccttccggcg agggggtgga ggaggggggt aaccgaaccg gtgaacccga agggagcctc
    10441 gtcccctccc cggaggagct gctcgttcgc ggcgagagcg actgcccgcc ttccatctgc
    10501 gaggcaggag acgcttgccg ggatccccag gacctgcttc tggaggatct ctcctggttc
    10561 ctcgctccac ctccgccacc gggagaggtc ctccggcgaa cggatgatcg tattgcgcca
    10621 gccccccgcg ccgctcctgg gcttgatgag gtaggggaaa tccttctcgc cggcgagggg
    10681 cggggcgggg atccccttcc cctcgaagaa tcgctggacc tcgatcttat cgaggagccg
    10741 tgcggcggtc ctccggtcgg tccccgcgag ggggatgggc gtcccgatgg actccgcccc
    10801 agacgtgggg atgaagaagt cgatccggtg gcggcccgcc atctcggcaa tccggtcggg
    10861 aagctcgtcg agctcctcga acttcagccg atcctccgtg taccacgaga ggtcctggtc
    10921 gcagaaatgg tcgacggcac agacggtgta tcccgccttt gatgcggact gggcgacgtg
    10981 gcgggtcgca aacccggcga tgaggaccgt cgcattcacg gcccggtcct cttgccgttc
    11041 cggctcgggt acacccggag ttccgcatcc gggtaggtcc ggccgagcgt agaaccctcg
    11101 aagaggtgat cgaggaagac ggcgaccccc gagacctccg agtggggctg gcccgtcacc
    11161 gagacgttgt aatcggcaag gccgtagatc tccccgggca ccttctcggc cccgacgacg
    11221 acaaggatct cctccgccat ccggatctcc ccgatggttt cggtcagggg aatcccgtac
    11281 atcgtgagat gcacaaccgt accccccttt tccttccagg agcggatgca tcccttccac
    11341 gagaccccgt cctcga
//
LOCUS       DATGDI010000184         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_106190, whole genome shotgun sequence.
ACCESSION   DATGDI010000184 DATGDI010000000
VERSION     DATGDI010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106190"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2477
                     /locus_tag="VKO45_06870"
     CDS             <1..>2477
                     /locus_tag="VKO45_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HMA05632.1"
                     /translation="EALKAAVSCGADAVYLGGKRFSARQYAANFDDQELGEAVEFAHG
                     RGARIYVAVNTLLHDREIPDAAEYLISLRETGVDAVLVQDPGLATLAREVIPDLPLHA
                     STQMTLHSREGLEWAATLGLRRVVLARELSLAEVECMGETARRLGIGLEVFAHGALCY
                     SWSGQCLLSSAIGGRSGNRGRCAQPCRRPYTLVRGGADEYGRPRRLAPVPLEDRYLLS
                     TKDLSLYPFLDRVVRAPVAALKIEGRMRSPEYVATVTGIYRKALDAIAAGSRVPLEPG
                     SSRGERGPSGAGTWTPSPADEEALALTFSRGFTGGCLCEGPDGIMGRDAPGHRGVLAG
                     VVQSVDPESGSVGIRLTGSAIPEKGDGLVFRVHGGREEEIGMVLRQGPAIDAGILWLR
                     TGPEAAEGVAEGAGVFITRRARLAGGGGCFSAGTPAPRWPRIRVDLALRVGGDGRPVL
                     EGSVIRNGVPLTVISRGGGFTVEAARARELTGREVGEILSRRGDAPFSIGKISLEWPA
                     GTYARKGDIGGLRRDLLAEVERYLVASGRPEPGEAVRARERLAGSPGEGSTGESPKGE
                     QALVILADTITAVEGLVEEGSGYIALEPSGAAAPPTGNTRVATPVEMAHGIVGEAATV
                     ARGSGWTLLWKWPRITGDPWIDLTGRFSGDPEFPSPPGLMVEGPGAALAAGSIFPGLP
                     LHGGAGLNVWNARSVRVLSPRFSSLTLSPELSLQDLRELVPRARSTGVRTPLGFLVEG
                     NLEVMVSEDRLRGLLPERRGPDRGDRFVGLRDGTDRIFPVGEDACGRTRILNSVETCL
                     VDQLPALSSAGIDLLVIDARGRGPRYA"
BASE COUNT          362 a          803 c          919 g          393 t
ORIGIN      
        1 agaggccctg aaggcagcgg tctcctgcgg agcggacgcg gtgtacctcg ggggaaagag
       61 gttctcggcc cgccagtacg cggcgaactt cgatgaccag gagctcgggg aggccgtgga
      121 gttcgcccac ggaaggggag cgaggatcta cgtggccgtg aacactctcc tccacgaccg
      181 ggagataccg gacgcggcgg agtacctgat ctcccttcgg gaaacgggag tggatgccgt
      241 cctcgtccag gatccgggcc tcgccaccct tgcgagggag gtgatcccgg acctgcccct
      301 ccatgcctcc acccagatga ccctgcacag ccgcgagggc ctggaatggg cggccaccct
      361 cggtctccgg agagtcgtcc tcgcccggga gctttccctc gcagaggtgg agtgcatggg
      421 ggagaccgcg cggcgcctcg ggatcggcct tgaggtcttc gctcacgggg ccctctgcta
      481 ctcctggtct gggcagtgcc tcctctcctc tgccatcggg ggaaggagcg ggaaccgcgg
      541 ccggtgcgcg cagccgtgcc ggaggcccta caccctcgtc cggggcgggg ccgacgagta
      601 cgggcgtccc cgccggctgg ccccggtccc ccttgaggac cggtacctcc tttccacgaa
      661 ggacctctcc ctctacccgt tcctggaccg ggttgtacgg gccccggtcg ctgccctcaa
      721 gatcgaaggg aggatgcggt cacccgaata cgttgccacc gtgaccggca tctatcggaa
      781 ggcgctcgat gccatcgccg cgggatcccg ggtcccgctg gagccgggat catccagggg
      841 ggaaaggggc ccctctggag ctggaacctg gaccccgtca ccggccgacg aggaggccct
      901 cgccctcacc ttcagccggg ggttcaccgg agggtgcctc tgcgagggtc cggacgggat
      961 catgggccgg gatgcacccg gccaccgggg agtcctcgcg ggggtagtgc agtccgtgga
     1021 tccagaatca ggatccgtcg ggatccggct caccggatcc gcgatcccgg agaaagggga
     1081 cggcctcgtc ttccgggttc atggcggtag ggaggaggag atagggatgg ttctcaggca
     1141 ggggccggcc atcgatgcag gaatcctctg gcttcgcacc ggtcccgaag cagccgaggg
     1201 ggtggcggaa ggtgcagggg tcttcatcac aaggagagca cgcctcgccg ggggaggtgg
     1261 gtgcttctcc gccgggacgc ccgccccgag gtggccccgg atccgtgtcg atcttgccct
     1321 ccgggttggg ggagacggga ggcccgtcct tgaagggtcc gtgatcagaa acggtgttcc
     1381 tcttaccgtg atcagccggg ggggtggttt cacggtcgag gccgcccgcg cccgggagct
     1441 cacgggaagg gaggtggggg agatcctctc ccggcggggg gatgctccgt tctccatcgg
     1501 gaagatcagc ctcgaatggc cggcgggcac ctatgcgcgg aagggcgaca tcggcgggtt
     1561 gcgccgggat cttctcgccg aggtggagag atacctcgtg gcgtcgggaa ggccggagcc
     1621 cggtgaggcg gtacgggcga gagagcggct ggcgggatct cccggggagg ggtcgacagg
     1681 ggaatccccg aagggcgagc aggcccttgt catcctggcg gataccatca ccgcggtgga
     1741 ggggctcgtg gaggagggat cggggtatat cgccctcgag ccttcggggg cggctgcacc
     1801 cccgacaggt aacacccggg tcgccactcc cgttgagatg gcacacggga tcgtcgggga
     1861 ggccgcaacc gttgcccggg gctcaggctg gaccctcctg tggaagtggc cgagaatcac
     1921 gggggacccc tggattgact tgaccgggcg gttctccggg gatccggagt ttccgtcccc
     1981 tccggggctg atggtggagg ggcccggcgc ggcccttgcg gccggctcca tcttccccgg
     2041 cctgcccctc cacgggggtg cggggctgaa cgtctggaat gcccgctcgg tccgggtcct
     2101 ctcaccccgg ttttcctccc tcaccctctc acccgagctc tccctgcagg atctccggga
     2161 gctcgttccc cgggcgagga gcaccggggt tcgcaccccg ctcgggtttc tcgtggaggg
     2221 gaacctcgag gtcatggtga gcgaggaccg gctccgcggc ctgctcccgg aaaggcgggg
     2281 accggaccgg ggggatcggt tcgtcgggct acgggacgga acagaccgga tcttcccggt
     2341 cggggaggat gcatgcggaa gaacccggat cctcaactcc gtcgagacct gcctcgtcga
     2401 ccagctgccg gccctttcct ccgccgggat cgacctcctc gtcatcgatg cgaggggacg
     2461 ggggccccgg tacgcgc
//
LOCUS       DATGDI010000185         2706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_426267, whole genome shotgun sequence.
ACCESSION   DATGDI010000185 DATGDI010000000
VERSION     DATGDI010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426267"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VKO45_06875"
     CDS             <1..511
                     /locus_tag="VKO45_06875"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HMA05633.1"
                     /translation="RERLERSRRICGLVRRFIRDGETVMVYSSKELEANTRPLIDDLL
                     ASGNPVIVPIIVREDVSLRLSYLEDPSVLVLSTFGVPEPIGSEIPARAEEVDTIILPM
                     LGFDRRGGRLGYGAGYYDRFLARNPRIRRIGIAFACQEAEEIPCDENDIHMNLIITED
                     GIAYSDGAG"
     gene            514..1407
                     /gene="fhcD"
                     /locus_tag="VKO45_06880"
     CDS             514..1407
                     /gene="fhcD"
                     /locus_tag="VKO45_06880"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HMA05634.1"
                     /translation="MERNGAVILDTYAEAFPVWLARVVITAESPEWAYRAAFEATGFA
                     TSRIGCPCEAGIEGCVSPEETPDGREGVSIFICSEKKNLKAIVSARVAQCILPVPTAS
                     AFDGFPEAETRFFTGMHYFGDSYEERCTVGGRRCWRIPTMEGEYVGEERFGVVPGIAG
                     GNFLVMGRDRPSALAAAEAAAEEVSGLGGVITSFPGGIVASGSKVGCRNYRFPMPAST
                     NHPLCPALRGRIEGSQVPEGVGSIYEIVINGMDEESVKGAMREGIEAATGTGKVLFIG
                     ASNFGGKLGPYRFFLRELFTP"
     gene            complement(1474..1656)
                     /locus_tag="VKO45_06885"
     CDS             complement(1474..1656)
                     /locus_tag="VKO45_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HMA05635.1"
                     /translation="MATDPVCYGEVDEETASQVVTYKGEKYYFCTPSCRKKFEENPGK
                     YTKLGVPSRIDPGMTC"
     gene            1761..2126
                     /locus_tag="VKO45_06890"
     CDS             1761..2126
                     /locus_tag="VKO45_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMA05636.1"
                     /translation="MAETLISAKEAAERLGVSLTVMQTMIKHGEIKALRRGRLVKVSE
                     SDLKDLLDQKKGRMEGDLTMSELRSLDEFKQNLVRALDDRVVKQALQRCIGGAGFRKQ
                     FLEALDSPEVKRKITGIRK"
     gene            complement(2387..>2706)
                     /locus_tag="VKO45_06895"
     CDS             complement(2387..>2706)
                     /locus_tag="VKO45_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HMA05637.1"
                     /translation="DYDRKNPERYHQYKVLSGRERFIEKTWGKNRVVRVFSTPGCTGC
                     RAVKEFLKARNVEFTEIDMAADEAARNFVMEKTGFLGSPVVQIGDEFIVGFDPKKMAR
                     ALG"
BASE COUNT          481 a          844 c          894 g          487 t
ORIGIN      
        1 aagggagcgg ctcgagagga gccggcggat ctgcgggctc gtccgccgtt tcatccggga
       61 cggcgagacc gtgatggtct actcctcgaa ggagctcgag gcgaatacga gacccctcat
      121 cgatgatctc ctcgcctccg gaaacccggt catcgtcccc atcatcgtcc gggaggatgt
      181 gagcctcagg ctctcgtacc tggaggatcc ctccgtcctc gtcctctcca ccttcggggt
      241 tcccgagccc atcgggagcg agatcccggc ccgggcggag gaggtggaca ccatcatcct
      301 ccccatgctc ggcttcgacc ggaggggggg aagactcggg tacggcgcag gctactatga
      361 ccggttcctg gcgaggaatc cccggatccg gaggatcggc atcgcctttg cctgccagga
      421 ggcggaggag atcccctgcg atgagaacga catccacatg aacttgatca tcaccgagga
      481 cgggatcgcc tactccgacg gagctgggtg atcatggagc ggaacggcgc ggtaatcctg
      541 gacacctatg ccgaggcctt tcccgtctgg ctggcgaggg tggtcatcac cgcggaaagc
      601 ccggaatggg cgtacagggc ggcctttgag gcaaccggtt ttgcgacgtc ccgaatcggc
      661 tgcccctgcg aggcagggat cgagggatgc gtctcccctg aggagacacc cgacggccgg
      721 gagggggtct cgatcttcat ctgctcggaa aagaagaacc tgaaggcgat tgtctcggcc
      781 cgggtcgccc agtgcatcct ccccgtgccc accgcctcgg ccttcgacgg gttcccggaa
      841 gcggagaccc ggttcttcac cgggatgcac tacttcgggg attcctacga ggagcggtgc
      901 accgtcggcg ggaggaggtg ctggaggatc ccgaccatgg agggagagta cgtgggggag
      961 gaaaggttcg gggtcgtgcc cgggatcgcc ggagggaact tccttgtcat gggaagggac
     1021 cggccctcgg ccctcgccgc tgccgaggcc gcggcggagg aggtctcggg cctcgggggg
     1081 gtcatcacca gtttcccggg ggggatcgtc gcgagcggct cgaaggtcgg gtgcaggaac
     1141 taccggttcc cgatgcccgc gagcacgaac caccctctct gtccggctct cagggggagg
     1201 atcgagggtt cccaggtccc cgagggcgtg ggctccatct acgagatcgt catcaacggg
     1261 atggacgagg aatcggtgaa gggggccatg agggagggga tcgaggccgc gacggggacg
     1321 ggaaaggtcc tcttcatcgg ggcctccaat ttcgggggga agctcggccc gtaccggttc
     1381 ttcctgagag aactgttcac cccgtgaaag ggttccaggg ctacgccttc ttcctgtgcg
     1441 gctgctgacc cgcttttctc cccgttccct gaactaacag gtcatcccgg gatcgatcct
     1501 ggagggtact ccaagcttcg tgtacttccc ggggttctcc tcgaatttct tcctgcagct
     1561 cggggtgcag aagtagtact tctctccctt gtaggtgacg acctgcgacg ccgtctcctc
     1621 gtccacctcg ccatagcaga ccggatcggt tgccacttca catccctcct tcactccccc
     1681 atcatccccg gaccatatgt acctgacggc ctttgccatt cgggggaatc cttaagaaca
     1741 ccggggtgta tcctccccgc atggccgaga cactcatctc agcgaaggag gcggccgaaa
     1801 ggctcggcgt ctccctcacc gtgatgcaga cgatgatcaa gcacggggag atcaaggccc
     1861 tgcggagggg ccgtctcgtg aaggtctccg agtcagacct caaggacctc ctcgaccaga
     1921 aaaagggaag gatggagggg gacctgacga tgtcggagct ccggtccctc gacgagttca
     1981 agcagaacct ggtccgcgcc ctggacgacc gggtggtgaa gcaggccctc cagcggtgca
     2041 tcgggggagc cggtttccgg aagcagttcc tcgaggctct cgactctccc gaggtgaaga
     2101 ggaagatcac cggaatccgg aaatagcccg gccccgtttc cgggccccgt cgggtgaatc
     2161 cctgccgggc cccggtccgg tgtccacggg gaagaaacag gcacagtacc ggcattccca
     2221 gatgcccggg atcggggtct cacccgtgag gagagaaccg catccggggc accggaagtc
     2281 agggcgatgt tccatgggga ggaaaagagg ggcaggttta tcaggataag gccgggaggg
     2341 ggtgaaagtg gacccgggcc ggaggttggt cccggcctga tccccctcac ccgagggccc
     2401 ttgccatctt cttcgggtcg aacccgacga tgaactcgtc cccgatctgc acgacgggcg
     2461 aaccgaggaa cccggtcttt tccatcacga agttcctcgc cgcctcgtcc gccgccatgt
     2521 cgatctcggt gaactcgacg tttcttgcct tcaggaactc cttgaccgcg cggcacccgg
     2581 tgcatcccgg cgtggagaac acccggacga cccggttctt cccccaggtc ttctcgatga
     2641 atcgctcgcg accggagagg accttgtact ggtggtaccg ctcggggttc ttgcggtcat
     2701 agtcct
//
LOCUS       DATGDI010000186         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_367883, whole genome shotgun sequence.
ACCESSION   DATGDI010000186 DATGDI010000000
VERSION     DATGDI010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367883"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VKO45_06900"
     CDS             <1..966
                     /locus_tag="VKO45_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HMA05638.1"
                     /translation="LKPDIDAIITELEKTSYREELEKAAVQNAGLACLEVALRMDIAR
                     LFRRIYSLFEEEIEEKYARILLNRWDVHNIKTVLRGKNIHEPSSEILGCLIPAGELDE
                     ATLVELVKQPDVRGVIDLLATWRIAYSVPLTRHFKDFSEERDLAVMEYALDKFYFENA
                     LESLRGETYDDRSMREMVATEIDVTNLRSMLKMIRDRIPLEDAQEFLIPGGSLKVERL
                     TALMKAGTIKDALKQLAGSPYEFLGQVPEETFKQEKISVFEKELERFLIRKGTSWFFG
                     DPLSIAIAIGYVWAKFTEVTNIRIISRLKMADVAEKIIREELVHV"
     gene            959..1279
                     /locus_tag="VKO45_06905"
     CDS             959..1279
                     /locus_tag="VKO45_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HMA05639.1"
                     /translation="MYRFLVVTDPDTAPGFRLAGVEVIEASSQEEARKAINALVFKDD
                     TGIVAVNEEFIAALDQKLVDTIEKTYRPIMIPIPSRAKRIDRTSYIERMLQRAIGYNI
                     VVRR"
     gene            1285..>2372
                     /locus_tag="VKO45_06910"
     CDS             1285..>2372
                     /locus_tag="VKO45_06910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HMA05640.1"
                     /translation="MIEGVVARISGPVVMAHGMRGSKMYDVVKVGDEKLNGEIIRLDG
                     EEAVVQVYEDTSGLKIGEIVVNTGNPLSVELGPGLLSSIYDGIQRPLAVLVEKSGSFI
                     SRGISVPGLDRRRKWAFEPAVKTGDLVSGGDVIGTVPEFNMKHRIMVPPGISGTVQEI
                     RAGSFSVDDTVCTLGDGQKIQLLQRWGVRKGRPYQKKLDPTLPLITGQRVFDTIFPVS
                     KGGTAMIPGGFGTGKTVAEQTLAKWADADIIVYIGCGERGNEMTEVLAEFPALLDPRT
                     GKPLMERTILIANTSNMPVAAREASIYTGITIAEYYRDMGYDVALMADSTSRWGEALR
                     EVSGRLEEMPGEEGYPAYLATRLAAFYER"
BASE COUNT          504 a          640 c          776 g          452 t
ORIGIN      
        1 ctcaaacccg acatcgatgc catcatcacc gagctcgaga agacgtccta tcgggaggag
       61 ctggagaagg cagccgtcca gaatgcgggg cttgcctgcc tcgaggtcgc tctgcggatg
      121 gacatcgccc gcctgttccg aaggatctac tccctcttcg aggaggaaat cgaggagaag
      181 tacgcgagga tcctcctgaa ccgctgggac gtgcacaaca tcaagacggt ccttcgcggc
      241 aagaacattc atgagccctc atcggagatc ctggggtgcc tcatccccgc cggggaactc
      301 gacgaggcga ccctggtaga actcgtcaag cagccggacg tccggggcgt catcgatctt
      361 ctggccacct ggaggatcgc gtactcagtg ccattgaccc ggcacttcaa ggatttcagt
      421 gaggagaggg atctcgcggt gatggagtac gcccttgaca agttctactt tgagaatgcc
      481 ctcgaatcgc ttcgtggcga gacctatgat gatcgcagta tgcgggagat ggttgcgacc
      541 gagatcgatg taacgaatct ccgttcgatg ctgaagatga tccgcgaccg gattcccctc
      601 gaggacgcgc aggagttcct gatccccggt ggttccctga aggttgagag gctcacggcg
      661 ctcatgaagg cagggaccat caaggatgcc ctgaaacagc ttgcgggctc gccgtatgaa
      721 ttcctcggcc aggttcccga ggagaccttc aagcaggaga agatctcggt ttttgagaag
      781 gagctggaga ggttcctgat ccggaaggga accagctggt tctttggaga tcccctctcc
      841 atcgccatag ccatcggcta tgtgtgggca aagttcaccg aggtaacgaa tatccggatc
      901 atttcccggc tcaagatggc ggatgtggcc gagaagatca tcagggagga gctcgtccat
      961 gtataggttc ctcgtggtca cggatcccga taccgcgccg ggcttccgtc tcgccggtgt
     1021 cgaggtgatc gaggcctcga gccaggaaga ggcgagaaaa gccatcaatg ctctcgtctt
     1081 caaggacgat accgggatcg ttgccgtgaa tgaggagttc atcgcggctc tggatcagaa
     1141 gctggtggat accatcgaga agacgtaccg ccccatcatg atccccatcc cttcgcgggc
     1201 aaagaggatt gacagaacca gctatatcga acggatgctg cagagggcca tcgggtataa
     1261 catcgtggta aggaggtaga aggcatgata gagggagtcg tcgcacgcat ctctggcccc
     1321 gtggtgatgg cgcatgggat gagggggtca aagatgtatg atgtggtgaa ggtgggtgac
     1381 gagaagctga acggcgagat catccgtctc gatggcgagg aggcggtcgt ccaggtgtac
     1441 gaggatacct caggcctgaa gatcggggag atagtggtga acacggggaa cccgctctcc
     1501 gttgagctcg ggccgggcct tctctcctcc atctatgatg gcatccagag gcccctcgcc
     1561 gtgctcgttg agaagagcgg cagcttcatc tcccggggta tcagcgttcc tggccttgac
     1621 cggcgcagga agtgggcctt tgagcccgcg gtcaaaaccg gggacctcgt tagtggaggg
     1681 gacgtcatcg ggacggtccc cgagttcaac atgaagcacc ggatcatggt gccaccgggg
     1741 atctcgggaa ccgtacagga gatcagggct ggatcctttt cggttgacga taccgtttgc
     1801 accctcgggg acggacagaa aattcagctc ttacagcggt ggggggttcg gaaaggaagg
     1861 ccgtatcaga agaaacttga cccgacgctg cccctcatca ccgggcagcg ggtctttgat
     1921 acgatatttc ccgtgagcaa ggggggcacg gcgatgatcc ccggcggctt cggcacgggt
     1981 aagacggtgg cagagcagac ccttgcgaag tgggcagacg ccgacatcat cgtctacatc
     2041 ggctgcggcg agcgtggtaa cgagatgacc gaggtgctcg ccgagttccc cgcactcctg
     2101 gacccgagga cgggtaagcc cctgatggag cggaccatcc tcatcgcgaa cacgagcaac
     2161 atgccggtcg ctgcccggga ggcctcgatc tataccggca tcaccattgc cgagtactat
     2221 cgggacatgg ggtatgatgt ggccctgatg gccgactcga cctcgcggtg gggagaggcg
     2281 ctgcgggagg tatcagggcg cctggaggag atgccagggg aggaggggta tccggcgtat
     2341 cttgccacgc ggcttgcggc cttctacgaa cg
//
LOCUS       DATGDI010000187         8236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_106389, whole genome shotgun sequence.
ACCESSION   DATGDI010000187 DATGDI010000000
VERSION     DATGDI010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 8236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8236
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106389"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VKO45_06915"
     CDS             <1..667
                     /locus_tag="VKO45_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HMA05641.1"
                     /translation="LLFLSVLTMTAGNLLALAQTNVRRILAYSSIAQMGYILLPFGIA
                     VTYGLATGILAALFYAVAYGLMKAGAFLSADLLSAASGTTEVAGMKGAGGRHPVTGIA
                     FSIFILGLIGVPATAGFAGKALIFLAGMSTLTWGGVILALVLAGNSILSLGYYVPLLS
                     TVLFTGHGEPGTGGITEKRIPPSGAIAVALLALATVLLGFFPLAVTGWLGSAAAFFPW
                     GVI"
     gene            668..997
                     /locus_tag="VKO45_06920"
     CDS             668..997
                     /locus_tag="VKO45_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05642.1"
                     /translation="MPLADILVSPFVIYAVAMGIGLLIYLWARRIAPPSTDVGDKRMQ
                     YVGGEAWEAQAYQPSYQFFYVAIFFTLAHVAALVIATAPPSASPLATIGYLAIIAVAV
                     MVLRWEQ"
     gene            1019..1555
                     /locus_tag="VKO45_06925"
     CDS             1019..1555
                     /locus_tag="VKO45_06925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HMA05643.1"
                     /translation="MTERADMSLSQRLATWARLKSPWITIFNSGACNACDIEIIATLT
                     PRFDVERFGILAKGSPRHADILIVTGPVTRKQAPRLRRIYEQMPEPKYVIAMGACGGR
                     GGVFQGLYHVMDEVEQVVPVDVYVAGCPPRPDEIINAVVETLRIAGEDLSRGGKEWRM
                     KRQPVEAAASEKKAEAGA"
     gene            1552..2028
                     /locus_tag="VKO45_06930"
     CDS             1552..2028
                     /locus_tag="VKO45_06930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HMA05644.1"
                     /translation="MTDLGMETAEAALAGFGPVARVRSNQVQVVTTLGMARDAIKAAL
                     GPISCDHLIQMSTADAGEHFELTYHLTGPHRLVISIRVNIPRGNPALPSVHDLLPPAG
                     IYERQIHDLFGIIFQGHPNLARIILNEDWPLKEYPLRKDWKPVKGTFYGGIREEVK"
     gene            2029..3201
                     /locus_tag="VKO45_06935"
     CDS             2029..3201
                     /locus_tag="VKO45_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HMA05645.1"
                     /translation="MVEITVPIGPQHPALKEPVNFKVFAEGETIVRLELDISYNHRGI
                     EKAAEQRDFIRVVPLLERICGICSHSHGTAFVKGVEEIMGVSASPRAQYIRTIIAELE
                     RMHSHFLWLGVAAHEIGFNTLFMWTWRDREVVQDILEELTGNRVNYGMNEIGGVRRDI
                     PPSQVKKTLSLLPRLEERLVKYTEMVKRQETMILRFRGVGKMTKAQALSFGTVGPTAR
                     ASGVDYDVRRDDPYLAYGEIPWKVVTSSDGDVYGRTVVRIGELGESIGMVRYCLEHLP
                     DGSITVEAPKKAPVGEILSRYEAPRGELAHYIKTNGTDRVDRLDIRTPTLANWTSVAM
                     CLVNQNIADIPVIVAAIDPCLSCTSRMTVVGTEGRARPGLSCTSRITVVKDSGGED"
     gene            3201..4169
                     /locus_tag="VKO45_06940"
     CDS             3201..4169
                     /locus_tag="VKO45_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HMA05646.1"
                     /translation="MDPFFLVLYILVFPGFLFLAAYALFLAYLDRKVAARMQHRVGPP
                     LLQPLADLIKVLGKEVIDPRGVDRPAFDAAPVLALAAVMTAFLYVPVIGPSPLAFPGD
                     LVLVLYLLTLPTIALFLVGWLSRNVFSTVGGVRAVTQLFIYEVPFFLALLAPAIMAGS
                     WSVSDITAFQANHPWFALLQPIGFVVALVGLQAKLERTPFDIPEAETEIVAGPWTELT
                     GRRLALMCLTNEMALVAGAALVAALFLGGPSVPGWMPAGAIAAIAGFLFFLAKTIAIL
                     LVLASVKVMTGRIRIDQLNNVGWKWLANAALLQIALVLIMTVWGVV"
     gene            4170..4577
                     /locus_tag="VKO45_06945"
     CDS             4170..4577
                     /locus_tag="VKO45_06945"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA05647.1"
                     /translation="MATVYEILVDAGDRFSVLREMIRNLFSKPATICYPFEKVPVPEG
                     FRGRVEYVDENCIGCGRCSRVCPAEAIVMVEGPREMEVKGKKLMRKKKPQVAIYRCIR
                     CGLCEEYCPQEPKAIRLTCEFSGAGADREVVVG"
     gene            4574..5068
                     /locus_tag="VKO45_06950"
     CDS             4574..5068
                     /locus_tag="VKO45_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HMA05648.1"
                     /translation="MRCSDSPGFEPVLRERLAHALPLAVVGIGDELNPHDRLGMLAAG
                     GIEALGLRGVRVFLAGTMPESITAPVRRFKPAHILFLDAAVMGKEPGTLGIIEACEVK
                     GDLLVTHALPLPVVMEYLEKEARAPVTLVGIQPDLASSGERPTPEEEKGIGGLASLLA
                     GILS"
     gene            complement(5084..5842)
                     /gene="mobB"
                     /locus_tag="VKO45_06955"
     CDS             complement(5084..5842)
                     /gene="mobB"
                     /locus_tag="VKO45_06955"
                     /inference="COORDINATES: protein motif:HMM:NF015181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HMA05649.1"
                     /translation="MKVIPVAGLSGSGKTTFIRNLIPLLREFGPVGTVKHAGRHGLDL
                     PPGKDTTVMFGAGAAAVAGIDGEKMLLTLGDASLATALDILSERGVATAVVEGWKSIP
                     WPKVVIGDLETDGCILRNPGPAEVIRQRDRFPDYFTVGEILRELGEGRREKGQACTTA
                     TATIPLPAGLKEDSLSSLESALPGFVNGMKNLPGAPGVRAAIRRGSLVGGTDEILIAV
                     AAGSGEEAATVLQIALSRLRKILDGTLIPVRRRD"
     gene            complement(5853..6458)
                     /gene="pdxT"
                     /locus_tag="VKO45_06960"
     CDS             complement(5853..6458)
                     /gene="pdxT"
                     /locus_tag="VKO45_06960"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HMA05650.1"
                     /translation="MGVRIGVLDLQGDVSEHVGAFREALAESGLSTGSGVVPVRKAGQ
                     IPSLDGLAIPGGESSTISKLIDKNGMREPLREFRGGIFATCAGLIVTAKEVPDPRVKP
                     LGILDIDVERNAFGRQKDSFEADLPIRGLETPFHAVFIRAPVISRVGDGVEVLATVDK
                     APVAVRQGKHLGLSFHPELSGDRRLHRMFLEGIQEPDGELP"
     gene            complement(6448..7344)
                     /gene="pdxS"
                     /locus_tag="VKO45_06965"
     CDS             complement(6448..7344)
                     /gene="pdxS"
                     /locus_tag="VKO45_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HMA05651.1"
                     /translation="MKLEELRFGTELLKRGFASMQKGGVIMDVVNAEQAVIAEEAGAV
                     AVMALERVPAEIRKAGGVARMADPARVTEIMDAVSIPVMAKVRIGHFVEAQVLESLGV
                     DMIDESEVLTPADEQFHIDKSLFSVPFVCGARDLGEALRRIDEGAAMIRTKGEAGTGN
                     VVEAVRHMRNIMGEIRALQGKSTQEMMDRAREIEAPAGLVLDVAKRGRLPVVNFSAGG
                     IATPSDAAMMMQLGADGVFVGSGIFLSQNPQQMAKAIVEAVNHYDDPRTIAEVSKNLG
                     EAMKGLDIHLLKPDEVLQTRGR"
     gene            complement(7364..>8236)
                     /gene="cbiB"
                     /locus_tag="VKO45_06970"
     CDS             complement(7364..>8236)
                     /gene="cbiB"
                     /locus_tag="VKO45_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HMA05652.1"
                     /translation="RVLGDPRSRFHPVAVLGRLIGWWGDPGRWAPGIQRTAGVVCGLG
                     TACLFALPFLLFDRFAPLWLFLLAGPFLLKVTFSWRALVEHALTVERAVKEDLGEGRR
                     AAGRMVSRDTGALSREETLSAAYESLSENLVDSITAPLLFFAVFSPLGLGLAAAAFYR
                     AANTMDAMLGYRDERERIGWFPARMDDVLTWIPARVTGLILLAYFAARGRFSPALSAL
                     RRDARRRPGLNGGIPMSIMAGGTGVRFEKPGVYTMGPGERSLAEGGPAILTALRAATL
                     LLAAILVAALLLWL"
BASE COUNT         1325 a         2887 c         2627 g         1397 t
ORIGIN      
        1 cctcctgttc ctctcggtcc tcaccatgac cgcggggaac ctccttgccc tcgcccagac
       61 caacgtgcgc cggatcctcg cctactcgag catcgcccag atgggctaca tcctccttcc
      121 cttcgggatc gccgtcacct atggcctcgc caccgggatc ctcgcggccc tcttctacgc
      181 cgtggcctat ggcctcatga aggccggggc cttcctttcc gccgaccttc tctccgcggc
      241 ctcgggcacc acggaggtcg ccggcatgaa aggggccggg gggcgacatc cggtcaccgg
      301 gatcgccttc tccatcttca tcctcgggct catcggcgtt cccgccaccg cgggctttgc
      361 gggaaaggcc ctcatcttcc tcgcgggtat gtccaccctc acctggggcg gagtgatcct
      421 cgccctcgtc cttgcgggaa acagtatcct ctccctgggc tactatgtcc cgctcctctc
      481 gacggttctc tttaccggtc atggggaacc gggcaccggc gggataacgg agaagaggat
      541 ccccccttcc ggtgccatcg cggtcgccct cctcgccctg gcgacggtcc tcctcgggtt
      601 cttccccctg gcggtgaccg ggtggctcgg gagcgcggcg gccttctttc cctggggggt
      661 gatctgagtg cccctcgccg acatcctcgt ctcccccttc gtcatctacg cggtcgccat
      721 ggggatcggc ctcctcatct acctctgggc gaggaggatc gcgcccccgt ccaccgacgt
      781 gggtgacaag cggatgcagt acgtcggcgg cgaggcctgg gaggcccagg cctaccagcc
      841 gtcctaccag ttcttctacg tggccatctt cttcaccctc gcccacgtgg ccgccctcgt
      901 catcgccacg gccccaccgt cggcgagccc cctcgcgacg atcggatacc tcgccatcat
      961 cgcggtggcc gtcatggtgc tgcggtggga gcagtgatgc tatcatggag gacgagagat
     1021 gactgagaga gcggacatgt ccctgtcaca gcgccttgcc acctgggcac gcctgaagtc
     1081 gccctggatc accatcttca acagcggggc atgcaatgcc tgcgatatcg agatcatcgc
     1141 caccctgact ccccgcttcg atgtcgagcg tttcggtatc ctcgcgaagg gctccccgcg
     1201 ccacgcagac atcctcatcg tgaccggccc ggtcacgagg aaacaggcgc cgcgccttcg
     1261 gcggatctac gagcagatgc ccgagcccaa gtacgtcatc gccatgggtg cctgcggcgg
     1321 gcgtggcggt gtcttccagg ggctctatca cgtgatggac gaggtggagc aggtggtccc
     1381 cgtggacgtc tacgtggcgg ggtgcccgcc ccggcccgac gagatcatca atgccgtggt
     1441 cgaaaccctc cgcatcgcgg gggaggacct gtcccgggga ggaaaggagt ggcggatgaa
     1501 gaggcagccc gtagaggccg ccgcatcgga gaagaaggca gaggcgggtg catgacggac
     1561 ctcggcatgg agacggcaga ggcggccctc gccggattcg ggccggtcgc ccgggtgcgg
     1621 tcaaaccagg ttcaggtggt gacgacgctc ggaatggccc gcgacgccat caaggccgcc
     1681 ctcgggccca tctcctgcga ccacctcatc cagatgagca cggcggatgc gggggaacat
     1741 ttcgagctca cctatcacct caccggtccc caccggctcg tgatctccat ccgggtgaac
     1801 atcccgcggg gaaaccccgc ccttccctcg gtgcacgacc tcctcccgcc ggcaggcatc
     1861 tacgagcgcc agatccacga cctcttcggg attattttcc aggggcaccc gaatctcgcg
     1921 cggatcatcc tgaacgagga ctggcctctg aaggagtatc ccctgaggaa ggactggaag
     1981 ccggtgaagg ggaccttcta cggaggcatc cgggaggagg tgaagtagat ggtggagatc
     2041 accgtcccca tcgggcccca gcacccggcc ctcaaggaac cggtgaactt caaggtcttt
     2101 gccgaggggg agaccatcgt ccggctggag ctcgatatct cctacaacca ccgggggata
     2161 gagaaggccg ccgagcagcg ggacttcatc cgggtcgtgc ccctcctgga gcggatctgc
     2221 gggatctgct cccactcaca cgggaccgcg ttcgtgaagg gcgtcgagga gatcatgggg
     2281 gtttcggcct cgccccgcgc ccagtacatc cgcaccatta tcgccgagct ggagaggatg
     2341 catagccatt tcctctggct cggggttgcc gcccatgaga tcggcttcaa caccctcttc
     2401 atgtggacct ggcgggaccg ggaggtggtc caggacatcc tggaggagct gaccgggaac
     2461 cgggtgaact acgggatgaa cgagatcggg ggggtcaggc gcgacatccc cccttcccag
     2521 gtgaaaaaaa ccctttccct cctcccgagg ctcgaggagc ggctcgtgaa gtacaccgag
     2581 atggtgaagc ggcaggagac catgatcctc cggttccggg gagtggggaa gatgacgaag
     2641 gcccaggccc tcagcttcgg gacggtcggg cccaccgccc gggcgagcgg ggtcgactac
     2701 gatgtccgga gggatgaccc ttacctcgcg tacggggaga tcccctggaa ggtcgtgacc
     2761 tccagcgacg gggatgtgta cgggaggacc gtcgtccgga tcggggaact cggcgagagt
     2821 atcgggatgg tccggtactg cctcgagcac ctccccgacg gctccatcac cgtcgaggct
     2881 ccgaagaagg ctccggtcgg ggagatcctc tcccggtacg aggccccgag gggggagctc
     2941 gcccattaca tcaagacgaa cggcactgac agggtcgaca ggctggacat ccgcaccccg
     3001 accctcgcca actggacctc tgtcgccatg tgcctcgtga accagaacat cgcggacatc
     3061 ccggtcatcg tcgcggccat cgatccctgc ctctcgtgca cctcccgtat gacggtcgtt
     3121 ggcacggaag gccgggcacg gcccggcctc tcctgcacct cgcggatcac cgtcgtgaag
     3181 gactccggag gtgaggactg atggatccct tcttcctcgt cctctacatc ctcgtcttcc
     3241 cggggttcct cttcctcgcc gcatacgccc tcttcctcgc gtacctggac cggaaggtgg
     3301 cggcccggat gcagcaccgg gtcggcccgc ccctcctcca gcccctcgcc gacctcatca
     3361 aggtcctcgg gaaggaggtc atcgacccga ggggagtgga ccgcccggcc tttgatgcgg
     3421 caccggtcct cgccctcgcg gcggtcatga ccgcgttcct ctacgtgccg gtgatcggcc
     3481 catcgcccct cgccttcccg ggggacctcg tcctcgtgct ctacctcctc accctcccga
     3541 ccatcgccct cttcctcgtg ggatggctct cccggaacgt cttctccacc gtcgggggcg
     3601 tccgggcggt gacccagctc ttcatctacg aggtgccctt cttcctcgcc ctcctcgccc
     3661 ccgccatcat ggccgggtcc tggtccgtct cggacatcac cgccttccag gcgaaccatc
     3721 cctggttcgc cctccttcag cccatcggct tcgtcgtcgc cctcgtgggg ctccaggcca
     3781 agctcgagcg cacgcccttc gacatccccg aggccgagac cgagatcgtg gccggcccct
     3841 ggaccgagct tacgggccgc aggctcgccc tcatgtgcct cacgaacgaa atggccctcg
     3901 tcgccggggc cgccctcgtc gcggcccttt tcctcggggg accctcggtg cccgggtgga
     3961 tgcccgcggg agccatcgcc gccatcgccg ggttcctctt cttcctcgca aagaccatcg
     4021 ccatcctcct cgtcctcgcc tcggtgaagg tcatgaccgg gaggatccgg atcgaccagc
     4081 tgaacaacgt gggctggaaa tggctcgcga acgccgccct cctccagatc gccctcgtcc
     4141 tcatcatgac cgtctggggg gtggtgtagg tggccaccgt gtacgagatc ctcgtcgatg
     4201 ccggtgaccg gttctcggtg ctcagggaga tgatccggaa cctcttttcg aagcctgcca
     4261 ccatctgcta ccccttcgag aaggtcccgg ttcccgaggg tttccggggg cgggtcgaat
     4321 acgtcgacga aaactgcatc gggtgcggcc ggtgctcccg agtctgcccc gccgaggcca
     4381 tcgtcatggt cgagggcccg cgggagatgg aggtcaaggg gaagaaactc atgaggaaga
     4441 agaagcccca ggtcgccatc taccgctgca tccggtgcgg cctctgcgag gagtactgcc
     4501 cccaggaacc gaaggccatc cggctcacct gcgagttctc gggagccggg gccgaccgcg
     4561 aggtcgtggt cgggtgaggt gcagcgactc ccccggcttc gaacccgtcc tccgcgagag
     4621 gctcgcccat gcccttcccc tcgcggtggt cgggatcggg gacgagctga acccccatga
     4681 ccggctcggg atgcttgccg cgggggggat cgaggccctc gggctccggg gcgtgagggt
     4741 tttcctcgcc gggacgatgc ccgagagcat caccgccccg gtccgtcgct tcaaacccgc
     4801 ccacatcctc ttcctcgacg cggccgtgat ggggaaggag ccgggcaccc tcggcatcat
     4861 cgaggcctgc gaggtgaaag gagacctcct cgtcacccac gcccttccgc tccccgtcgt
     4921 gatggagtac ctggagaagg aggcgagggc accggtcacc ctcgtcggga tccagcccga
     4981 cctggcctcg tccggagaaa gaccgacccc ggaggaggag aaggggatcg gggggctcgc
     5041 ctcgctcctg gcagggatcc tctcttaatc catctccctg aagtcagtct ctgcgacgca
     5101 caggtatcag agttccgtcc aggatcttcc tcagccggga cagggcgatc tgcaggaccg
     5161 tcgcggcctc ctcaccactc cccgcggcga ccgcgatgag gatctcgtcg gtgcccccga
     5221 caagagagcc ccgccggatg gcggcccgga caccgggggc gcccgggagg tttttcatcc
     5281 cgttcacaaa tccgggcagc gcgctttcga gggaggagag ggagtcctct ttcaacccgg
     5341 caggcagagg gatggtggcg gtcgccgtgg tgcaggcctg tcccttttcc ctgcggcctt
     5401 ccccgagctc gcgcaggatt tccccgacgg tgaagtaatc ggggaaccgg tcccgctgcc
     5461 tgatcacctc tgccgggcca gggttccgga gaatgcaccc gtcggtctcg agatccccga
     5521 tgaccacctt cggccagggg atactcttcc agccctccac cacggccgtt gcaacccccc
     5581 gctctgagag gatatcgagg gcggtggcga gggaggcgtc cccgagggtg aggagcatct
     5641 tctccccgtc gatcccggcg accgccgcgg cgcctgcccc gaacatgacg gtggtatcct
     5701 tcccgggcgg cagatccagg ccatggcgcc cggcatgctt cacggtgccc accggcccga
     5761 actcccggag gagagggatg aggttccgga tgaaggtcgt ttttcccgaa cccgacagtc
     5821 ctgcgaccgg gatgaccttc accgtgcacc tctcatggca gttctccgtc cggttcttgg
     5881 ataccttcga gaaacatccg gtgcagcctc ctgtcccccg agagctccgg gtggaagctg
     5941 agtccgagat gcttcccctg gcgtaccgcg acgggcgctt tgtccaccgt ggcaagcacc
     6001 tccaccccgt caccgacacg ggagatgacc ggtgcccgga tgaacaccgc atggaagggg
     6061 gtctccaggc cccggatggg gagatcggcc tcgaaggaat ccttctgccg gccgaaggcg
     6121 ttcctctcca catcaatatc gaggataccg aggggcttca cccggggatc ggggacttct
     6181 tttgccgtga cgatgaggcc ggcgcaggtg gcgaagatcc cgccccggaa ctcccggagc
     6241 ggttccctca tcccgttctt atcgatgagc ttcgagatgg tagaggattc gccgcccgga
     6301 atggcgagcc cgtcgagtga tgggatctgg ccggccttcc tcacgggaac gactccggat
     6361 cccgtggaga gaccggattc ggcaagggcc tcccgaaagg ccccgacgtg ctccgagaca
     6421 tctccctgga ggtcgaggac gccgatccta acgcccacgg gtttggagca cctcgtccgg
     6481 cttcaggagg tggatgtcga ggcccttcat ggcctcgccc aggtttttcg agacctcggc
     6541 gatggtgcgg gggtcgtcat agtggttcac ggcctcgacg atggccttcg ccatctgctg
     6601 cgggttctgg gagaggaaga tcccggatcc cacgaagacc ccgtcggccc cgagctgcat
     6661 catcatcgcg gcgtccgagg gggtggcgat cccgcccgcc gagaagttca cgaccggaag
     6721 ccggccgcgc tttgccacgt cgaggacgag gcccgccggt gcctctatct cccgggcccg
     6781 gtccatcatc tcctgggtgg acttgccctg cagggcccgg atctcgccca tgatgttccg
     6841 catgtgccgg accgcctcga ccacgtttcc cgtcccggcc tcccccttgg tccggatcat
     6901 ggccgcaccc tcgtcgatcc ggcggagggc ctccccgagg tccctggcgc cgcagacgaa
     6961 ggggacggag aagaggctct tgtcgatgtg gaactgctca tcggcgggcg tgagcacctc
     7021 gctctcgtcg atcatgtcca ccccgaggga ctcgaggacc tgcgcctcca cgaagtggcc
     7081 gatccgcacc ttcgccatga ccgggatgga gaccgcgtcc atgatctccg tgacccgggc
     7141 cgggtcggcc atccgcgcga cccccccggc cttcctgatt tccgccggca cccgctccag
     7201 ggccatgacg gccaccgccc ctgcctcctc ggcgatgacc gcctgctcgg cgttcaccac
     7261 gtccatgatg accccgccct tctgcatgga cgcaaagccg cgcttgagga gctcggtgcc
     7321 gaacctcagt tcctccagct tcatagcatg atccatacgc gggtcaaagc cataataata
     7381 gcgcggcaac gaggatggcc gcgaggagga gcgtggccgc ccggagggcc gtgaggatcg
     7441 ccgggcctcc ctccgcgaga ctcctttccc cgggccccat ggtgtacacc ccgggcttct
     7501 cgaaccggac cccggtcccc cctgccatga tggacatggg gatccccccg ttcagccccg
     7561 gccgcctccg ggcatccctc ctgagcgcgg agagggccgg ggaaaacctc ccccgggcgg
     7621 cgaagtatgc gagcaggatg agcccggtca cccgggccgg gatccaggtg aggacatcgt
     7681 ccatccgggc ggggaaccac ccgatccgct cacgctcgtc ccggtagccg agcatcgcgt
     7741 ccatcgtgtt cgccgcccgg tagaaggcag cggcggcaag cccgagaccc agaggggaga
     7801 agacggcgaa gaagaggagg ggggccgtga tgctgtctac aaggttctcg gagagggact
     7861 cgtaagcggc ggagagggtc tcctcccggg acagggcgcc cgtatcccgg ctcaccatcc
     7921 tccctgcagc cctccgccct tccccgaggt cctctttcac cgcccgctca acggtgaggg
     7981 catgctccac cagcgcccgc cacgagaagg tcaccttgag gagaaacggg cctgcaagga
     8041 ggaagagcca gagaggggcg aaccggtcaa agaggagaaa gggaagggcg aagaggcacg
     8101 cggtcccgag cccgcacacg acaccggcgg tcctctggat gccgggggcc cagcggccgg
     8161 ggtcgcccca ccagccgatg aggcgcccga ggaccgcgac ggggtggaaa cggctccggg
     8221 ggtccccgag gacccg
//
LOCUS       DATGDI010000188         1898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_426485, whole genome shotgun sequence.
ACCESSION   DATGDI010000188 DATGDI010000000
VERSION     DATGDI010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426485"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VKO45_06975"
     CDS             <1..361
                     /locus_tag="VKO45_06975"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HMA05653.1"
                     /translation="YIPPQTAGQTVGRGGTRSFTLVADKTGTGNITTEYRRPWVPAGT
                     VTRVNLEGGFFGILGDDGKKYEPQNLDTRYQVDGLRVAFEATAATGTVSMRMWGTPVT
                     LDSIETIHGFSLRVPVK"
     gene            554..1270
                     /locus_tag="VKO45_06980"
     CDS             554..1270
                     /locus_tag="VKO45_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05654.1"
                     /translation="MPDFRTYLIASCLLVLCMLFAGCTGGGNQGATPSPTMGGGTAPG
                     LVITSPANGAVLPAGDITVSVKVSNFRLVPSYGQPAVAGEGHFHYYMDLPVPLVEKKV
                     GVTPPGSFVPTTDTSHTFTNVPAGTHNFSVELATNDHSPFPQPIFATVTVTVTGQLPV
                     TTGTTVGGQPDLQACTTDSDCVPNLCCHPTNCINRAYKGVCTELCTNVCSGPIDCGAG
                     HCACVKGRCGVSPGRAPGTM"
     gene            1290..1595
                     /locus_tag="VKO45_06985"
     CDS             1290..1595
                     /locus_tag="VKO45_06985"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HMA05655.1"
                     /translation="MAFIKVGTAADFLFDRMTGIEESGKDVVIAKAGGRFHALGNRCT
                     HEGCRLSNGRIWDGKIRCPCHGSLFDPATGKVLQGPAEKPLPVYHIKVEGGQVWVDI"
     gene            1681..>1898
                     /locus_tag="VKO45_06990"
     CDS             1681..>1898
                     /locus_tag="VKO45_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HMA05656.1"
                     /translation="MIRLKRVYDPPSEEDGYRILVERLWPRGISKDRAKIDLWLKDAG
                     ASTELRTWYGHDPQRWKEFRRRYFGEIR"
BASE COUNT          358 a          607 c          600 g          333 t
ORIGIN      
        1 gtacatccct ccccagaccg cggggcagac cgtgggacgg gggggcaccc ggtccttcac
       61 cctggtggcg gataagaccg gcaccgggaa tatcaccaca gagtacaggc gcccctgggt
      121 gcccgcgggc accgtgaccc gagtaaacct cgaggggggg ttcttcggga tcctcggtga
      181 cgacgggaag aagtatgagc cccagaacct ggatacccgg taccaggtgg acgggctccg
      241 ggtcgccttc gaggcgacgg ccgcaacggg cactgtcagc atgaggatgt ggggaacccc
      301 ggtgaccctg gactccatcg agacgataca cgggttctcc ctcagggtgc cggtgaagta
      361 aagcgccgat attccctccc cttcctcctt tttcgcgacc attcctcatc ccttcgccgt
      421 gtccggatgc cccgctatcg gagggagaac cgatttttta aggaaaatta atggcatggg
      481 aggccaatca gtcccctgcc ggttttcccg gttccgggat aaatttatgg ggaaggagta
      541 cgatgctgtc cccatgcccg acttccgaac atacctgatc gcctcctgcc tcctcgtcct
      601 ctgcatgctc tttgcggggt gtacaggagg agggaaccag ggtgccacgc cgagcccgac
      661 catgggcggg ggaacggcac caggtctcgt catcacctct cccgctaacg gtgccgtgct
      721 ccccgccggg gacatcaccg tttcggtgaa ggtctccaac ttcaggctcg tcccgagcta
      781 tgggcaaccg gccgtggccg gggaggggca ctttcactat tacatggacc ttccggttcc
      841 cctggtggag aaaaaggtcg gcgtgacccc gcccggaagc ttcgtcccca ccaccgacac
      901 cagccatacc ttcacgaacg tccccgcagg aacccataat ttctcggtgg agctcgccac
      961 gaacgaccat tcccccttcc cccagcccat cttcgccacc gttaccgtca cggtcaccgg
     1021 ccagctgccc gtgaccacgg ggacaaccgt ggggggacaa ccggatctcc aggcctgcac
     1081 gaccgacagc gactgcgtgc caaacctgtg ctgccatccg acaaactgca tcaaccgggc
     1141 gtacaagggt gtctgcaccg agctgtgcac gaatgtctgc tccgggccca tcgactgcgg
     1201 ggcgggacac tgcgcttgcg tgaaagggag atgcggggtg tctcccggca gggcacctgg
     1261 aacgatgtga ggtcccggga gagcaggata tggccttcat caaggtcggg acagcggctg
     1321 acttcctctt cgaccggatg acggggatag aggagtccgg aaaggatgtc gtcatcgcga
     1381 aggccggggg gcggttccat gcccttggca accgctgcac ccacgaggga tgccggcttt
     1441 ccaacgggag gatctgggac gggaagatcc ggtgcccctg ccacgggtcc ctcttcgatc
     1501 ccgcgaccgg gaaggtcctc cagggccctg ccgaaaagcc cctcccggtg taccacatca
     1561 aggtcgaggg agggcaggtc tgggtggata tatgactctc ccgctctggg aaggaatctt
     1621 ctccacggcg gtttccctcg ttccctgcag aaatccgaac ccatgggccg ggtgcacccg
     1681 atgatccgtc tcaagcgcgt gtacgatccc ccgtcggagg aggacgggta ccggatcctc
     1741 gtggagcgtc tctggccccg gggcatctcg aaggatcgcg cgaagatcga cctctggctg
     1801 aaggatgcgg gggcgagcac ggaacttcgc acctggtacg ggcatgaccc gcagagatgg
     1861 aaggaattcc ggcggaggta cttcggggag atccggca
//
LOCUS       DATGDI010000189         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_368235, whole genome shotgun sequence.
ACCESSION   DATGDI010000189 DATGDI010000000
VERSION     DATGDI010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368235"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..2166
                     /locus_tag="VKO45_06995"
     CDS             184..2166
                     /locus_tag="VKO45_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05657.1"
                     /translation="MKENYRFALLLFLTSGIALAAILFMILLSTRPTLEARVPADITA
                     GVIAISLFFAGATVLQPLYLFYRLDRQKHGVPDRGFPGQSGPIPGAGSVAGGLGLVPH
                     YLSFAPTFTRSGYFPADERDKPWVVNLLFTGDPLDFDSNGLSATILDLQRRGFLELMP
                     GERGEIHSRVTLSEPGNLYESVELSSLLDPYEVQVLGFFRMAEDEERTKPGNKPPGAE
                     GAAWPSPPIRRLSPKGLYPPSGLFPAGDLLKAVSSRHFTDGRPGIVPFVLAAALLYTL
                     LLFALAWPWAPIIVIPVGLIISIVWMGAYAITKGFATAHPLPTPAERARWILFWTFFI
                     VWGISLPFLMAPSVRGDLSVFLDPMFWFIVALLVLISLVLTAGYLFLADPGSDRPFFM
                     IVALLLLLGGLILTVSVPDPRLLLATFISALSLALLFRSVIQKRAGMAFLSTILISVA
                     GIPAVATLLARDIGGMAPVSPVANNLTVNFAFACVVALFLTAQYFLFRGHFLQELREY
                     DPKKQTNPGTEAMFCFVLFLALFFFLQVYQSGIVLLVLALLVVFQYLTALSFPSTLFG
                     RWSRENERKKQEWDAFRNMLSDFAMIRQYAPEDLAMWGEWLVYGTALGAGENVERVMK
                     ERGIEIPGWVLPPDVPLPPELTANFRLARWWNRFSG"
     gene            complement(2117..2371)
                     /locus_tag="VKO45_07000"
     CDS             complement(2117..2371)
                     /locus_tag="VKO45_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05658.1"
                     /translation="MAPALEPSALGIFVFGLIAGICPCNNAVCLGLIGYLSVRKDRVP
                     PLRVLERRERANPAPSKPGMRASSLSQRTGSTTVPAGSSP"
BASE COUNT          376 a          874 c          732 g          561 t
ORIGIN      
        1 tgggggttcg cgcccccctc ccggggaggt atgggaggag gcgggttcca cggcggtggc
       61 ggattcggcg gtggcggagg cttcggagga ggcggggccg gggggaggtg acccggcccg
      121 gtaaaaggat aatcccccta atccaccatc ccttatcgaa aaaagctctc cgcgggctgc
      181 tccatgaaag aaaattaccg gttcgccctt ctcctgttcc tcacctccgg tatcgccctc
      241 gccgcgatcc tcttcatgat cctcctttcc acccggccga cgctggaggc gagggttccc
      301 gccgatatca ccgcgggggt catcgcgatc agcctcttct ttgccggtgc cacggtcctc
      361 cagcccctgt acctgtttta ccggctcgat cggcagaagc atggggttcc ggatcgcgga
      421 ttccccggtc aatccggccc cattcctggc gctggttcgg ttgccggcgg gctaggtctc
      481 gttcctcact acctgagttt tgccccgacc ttcaccaggt cggggtattt ccccgcagac
      541 gagcgggata aaccatgggt cgtcaacctg cttttcaccg gggatcccct ggacttcgat
      601 tctaacggac tttctgcgac gatcctcgat ctgcagcgcc ggggtttcct tgagctcatg
      661 cccggggaga ggggtgagat ccactcacgg gtcaccctgt cggaaccagg gaacctctat
      721 gaatcggtgg agttgtcatc cctgctcgat ccgtacgagg tgcaggtgct cggcttcttc
      781 aggatggccg aggacgagga aaggacgaaa ccggggaaca agccaccggg ggcggagggg
      841 gccgcctggc cctccccgcc catccggcgg ttatccccga aagggctata tcccccgagc
      901 ggcctcttcc cggcagggga tctccttaag gccgtgtcct cccggcattt taccgacggg
      961 aggccgggca tcgtcccctt cgtactcgcc gcggcgctcc tctacaccct gctcctcttt
     1021 gccctggcat ggccctgggc tcccatcatc gtgatacccg tcgggcttat catctccatc
     1081 gtctggatgg gggcctatgc cattacgaag ggtttcgcaa ccgctcaccc cctcccaacg
     1141 cctgcggaac gtgcgaggtg gatcctcttc tggacgttct tcatcgtctg gggcatttcc
     1201 ctccccttcc tcatggcccc gagcgtccgc ggggaccttt ccgttttcct ggatcccatg
     1261 ttctggttca tcgtggccct cctcgtcctc atctccctcg tcctgaccgc ggggtacctt
     1321 ttcctcgcag accccggctc ggatcgtccc ttcttcatga tcgtcgcgct gcttctcctc
     1381 ctcggcggcc tgatcctcac ggtatcggtt cccgatcccc gtctcctcct cgcaaccttc
     1441 atatcggccc tctcccttgc cctcctcttc aggtccgtga tccagaaacg cgcggggatg
     1501 gcattcctct cgacgattct catctccgtc gccggaatcc ccgcggttgc taccctcctc
     1561 gcccgggata tcggcggtat ggccccggtc tcgccggttg caaataacct gacggtgaac
     1621 ttcgcctttg cctgcgtggt cgccctcttc ctcaccgccc agtacttcct gttcaggggg
     1681 catttcctcc aagagctccg ggaatatgat ccgaaaaaac agacaaatcc ggggaccgag
     1741 gcgatgttct gtttcgttct gttcctcgcg ctgttcttct tcctccaggt ctaccagtcg
     1801 ggtatcgtcc tcctcgtcct tgctctcctc gtcgtgttcc agtacctcac cgccctctcc
     1861 ttcccctcga ccctgttcgg gcggtggtcc agggaaaacg aacggaagaa gcaggagtgg
     1921 gatgccttcc ggaacatgct ctcggatttc gccatgatca ggcagtacgc ccccgaggat
     1981 ctcgccatgt ggggcgagtg gctcgtctat ggtaccgccc tgggagccgg tgagaacgtg
     2041 gaacgggtga tgaaggaacg ggggatcgag atcccgggat gggtgctccc gcccgatgtc
     2101 cccctccccc cggagcttac ggcgaacttc cggctggcac ggtggtggaa ccggttctct
     2161 ggctgaggct ggaagccctc atccccggtt tgctaggggc cgggttcgcc cgttccctcc
     2221 tttccaggac ccggaggggc gggacccggt ccttccttac gctcaggtac ccgatgagcc
     2281 ccaggcagac ggcgttgttg catgggcaga tgcccgcgat gaggccgaag acaaagatcc
     2341 cgagggccga gggctcaagg gccggtgcca tcccattctc tccacccttc ggttctcacg
     2401 gcaggttccc gtcgcccatg cactcctgct gccgctcctc gctcatctcg acgaggatgc
     2461 cgaagatctt ctggaccgag acaggatcga ccttggcctc gacggcccgg tcaaaggccc
     2521 ggtcgaggac atgctccctc tgc
//
LOCUS       DATGDI010000190         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_106783, whole genome shotgun sequence.
ACCESSION   DATGDI010000190 DATGDI010000000
VERSION     DATGDI010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106783"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VKO45_07005"
     CDS             complement(<1..648)
                     /locus_tag="VKO45_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMA05659.1"
                     /translation="MKFLVTGGAGFIGSHRARELTQLGEVTILDDLSSGGTGRIEDLL
                     DQRKATLIRGSITHRTLLADACRGVDCVFHLAAIVSVARSMEQPMEVHEVNAGGTLAV
                     LSAARTAGVRKVVFSSSCAVYGDSSVTPKTEGLLPEPLSPYAASKLAGEYYCEVFRRS
                     LGLPTASLRYFNVYGPGQDPRGEYAAVIPRFAVNVLRRRPPVIFGDGNQVRDFISV"
     gene            complement(732..1640)
                     /gene="uvsE"
                     /locus_tag="VKO45_07010"
     CDS             complement(732..1640)
                     /gene="uvsE"
                     /locus_tag="VKO45_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HMA05660.1"
                     /translation="MRIGYPCINRTIGCTANHTFRLASYGRERLEATVGENLACLGRI
                     LSFNRDHGILFFRITSDLIPFASHPVMDVAWQDRFRDSFAEIGRIVRNSGIRISMHPD
                     PFTLLNSPDEAIVGRSIAELRYHAEVLDLMGLDATAKVQIHVGGVYHDREAAIRRFVG
                     RAAGLDECILRRLAIENDDLRFPLADCLRIHGETGLPVILDTFHHELNHRGETLPEAV
                     GAAAGTWSGPDGIPMVDYSSQEEGKRRGTHATTLVPENFMQFLRASRHHDLDVMLEIK
                     DKERSTLRALEIASNDPRLVLRSPHP"
     gene            complement(1637..>2081)
                     /locus_tag="VKO45_07015"
     CDS             complement(1637..>2081)
                     /locus_tag="VKO45_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05661.1"
                     /translation="IRRVFIHLFFPGQARVHDREIEPILEKTLIRKRPRDFYYALMDY
                     GSALGKGSGEPDGPSRRGKSGNPNARSASYIRQGPFEGSDRQARGIIIRRVLAEGEIR
                     EDRLGPSLEVGPERLERILRGLAKDGFVDREKGRIRCRETSGESR"
BASE COUNT          367 a          636 c          675 g          403 t
ORIGIN      
        1 taccgagatg aagtccctga cctggttccc gtccccgaag atcacgggag gtctccttcg
       61 gaggacgttc accgcgaacc tggggatcac cgcggcatac tcgcccctgg ggtcctggcc
      121 ggggccgtac acgttgaagt aacggaggga tgctgtcgga agcccaaggg agcgcctgaa
      181 cacctcgcag taatactccc cggcgagctt cgaggccgca tagggtgaga ggggctcggg
      241 caggagccct tctgtttttg gggtaacgga gctgtcgccg tagacggcgc atgaggagga
      301 aaagaccacc tttctcaccc cggcggtgcg ggccgccgag aggaccgcga gggtcccgcc
      361 cgcattcacc tcgtggactt ccatgggctg ttccatggat cgggccacgg agacgatggc
      421 cgcgaggtgg aagacgcagt ccaccccccg gcaggcatcg gcaaggaggg tccggtgggt
      481 gatactcccc cggatgaggg tggccttcct ctggtccagg aggtcctcga tccggcccgt
      541 tccgcccgaa gagagatcgt cgaggatggt cacctcgccg agctgggtga gctccctcgc
      601 gcggtgcgag ccgataaacc cggccccgcc ggtcaccagg aacttcatcg tgcctccggg
      661 agggtgttca tcatcacaag aaccttcccc atgtcttaaa agaacctttc ttttcggtat
      721 cgacctgccc ttcacggatg agggcttctg agaacaagcc ttggatcgtt cgatgcgatt
      781 tccagggccc ggagggtgct ccgctccttg tccttgatct cgagcatgac atccagatca
      841 tggtggcgtg aggcccggag gaactgcatg aaattttccg gaacgagagt ggtggcatgg
      901 gtccccctcc tctttccctc ctcctgtgag ctgtaatcca ccatcgggat tccgtcgggc
      961 ccggaccagg tcccggcggc tgcgccgacc gcctcgggga gggtttcacc ccggtgattc
     1021 agttcatggt ggaaagtgtc gaggatgacg ggcaggccgg tctccccatg aatccggagg
     1081 cagtctgcga gggggaaccg caggtcgtcg ttctcgatgg cgaggcggcg gaggatgcat
     1141 tcgtcgagtc ctgcggcccg gccgacgaac cggcggatgg ccgcctcccg gtcatggtac
     1201 actcctccga catggatctg gacctttgcg gtggcatcaa gtcccatgag atcgaggacc
     1261 tcggcatggt accggagttc tgcgatggat ctcccgacga tggcctcatc gggtgagttg
     1321 aggagggtga acgggtcggg gtgcatcgag atgcgaatcc ccgaattcct gacgatcctc
     1381 ccgatctccg cgaaagaatc cctgaaccgg tcctgccagg cgacgtccat gaccgggtga
     1441 gaggcgaagg ggatgagatc cgaggtgatg cggaaaaaga ggatgccgtg atcgcggttg
     1501 aaggacagga tcctccccag gcatgcgagg ttctcaccaa cggttgcctc gagccgttcc
     1561 cggccgtatg aggcgaggcg gaaggtgtgg ttcgcggtgc agccgatggt ccggttgatg
     1621 cacgggtacc cgatcctcat ctcgactccc cggaggtttc ccggcatcga atccttccct
     1681 tttcccggtc gacgaaccca tccttcgcga gtccccggag gatgcgctcc agccgttcgg
     1741 gaccgacctc gagtgaaggg cccaggcgat cctcccggat ctctccttcc gcgaggaccc
     1801 ggcggatgat gatcccccgc gcctgccggt cagacccttc aaagggcccc tgccttatgt
     1861 aggaagcgct ccgggcgttc gggtttcctg atttccctcg ccgtgaaggt ccgtccggtt
     1921 ctcccgaacc tttccctagg gcagagccat agtccatgag ggcatagtaa aagtccctcg
     1981 gtctcttcct gatgagggtt ttttccagga tcggttcgat ctcccggtca tggacccggg
     2041 cctggccggg gaagaacaga tggatgaaca cccggcggat g
//
LOCUS       DATGDI010000191         3958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_194113, whole genome shotgun sequence.
ACCESSION   DATGDI010000191 DATGDI010000000
VERSION     DATGDI010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194113"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VKO45_07020"
     CDS             <1..510
                     /locus_tag="VKO45_07020"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HMA05662.1"
                     /translation="GFPLTIYLLSGWLGSRVPGLDLLSHNAGHLWYTALGLSGDPHTN
                     VIHLASYVFIFGGLLLLMNAWSVLYAARRDDRLAVTGPYAYVRHPQYLAFILVMFGFL
                     LQWPTVLTIIMFPILVWRYVSLAREEEESVKAEFGNEYMEYLARTPAFIPRLFRSHTV
                     PPREEPGIP"
     gene            576..1394
                     /gene="artA"
                     /locus_tag="VKO45_07025"
     CDS             576..1394
                     /gene="artA"
                     /locus_tag="VKO45_07025"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase A"
                     /protein_id="HMA05663.1"
                     /translation="MIDIFALILLVSFLVYLIPGPARRFAAIAGWAAIILSLLAEVPE
                     YLSLDNLLYPLLAIASVPFLVMTAEFLLREDPRVMRLTDAAAIAVFLSAPFTLVPGLA
                     DILITLLIGQVAWLLHALGYHADFLAWNIIARNGFASEIILACTGITAIAIMLGVAGS
                     GRPTPRQGVLAFLLVVPAIYLLNLLRVSFVFIASSEQWFSFLPDLTSDPAPGYGAVSF
                     FWAHNVIAETLSVLALVGIAFGLFRIIPQLAAFAREVVDLYAGDIRAFIGKTGA"
     gene            1562..3667
                     /locus_tag="VKO45_07030"
     CDS             1562..3667
                     /locus_tag="VKO45_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HMA05664.1"
                     /translation="MAIDPVCGMEVDEEHAIKRVIGGRTYYFCMESCALRFADPEQEL
                     KSMKRRVAVAVSGVMVIAALRVIATLSLAAGATLITWAPIPQLPFFTWGYWLFLLTTP
                     VQFIGGWSFYRGAYAALSAHRANMDVLIAMGTSTAYFFSVFVLFFPGVLPVAGRDVYF
                     EVAAVIIAFVLLGKFMEDYMRSSTSAAVRKLMDLRPSTARVIRGGKEEEIPAEMVMVD
                     EVIIVRPGEKIPTDGVVAEGRSAVDEKMITGESIPVEKGPGDPVIGATLNKMGMMKVR
                     ATKIGSETTLMQIVRMVEEAQASSAPIQRLADRVAEYFVPAVIGVAVLTFFTWAVILG
                     NFTAGLLAFVAVLIISCPCALGIATPTALMVGVGKGAEAGILIRGGEYLERAQSLTTV
                     VFDKTGTLTKGEPSVTDILGEDQNEVIRLAAIAEKGSEHPLGEAIVRGAKERGIELKD
                     PESFEAVPGHGIRVVIGGKNVLVGNRRLMQANGIDTATYEEKIQALEVQGKTAMLVAV
                     NNGITGIIAVADTLKENSVEAVKELKSLGIETVMLTGDNERTAHAIAQQVGIEGVIAN
                     VLPGEKADVIKKLQTEKKVVAMVGDGINDAPALAQSDIGIAVGSGSDVAKETGGIVLI
                     RDDLRDVVAGIKLSKATMRKIRQNLFWALAYNTLAIPVAAVGLLNPIIAAAAMSLSSI
                     TVVGNAALLRRFRFEEKGA"
BASE COUNT          752 a         1201 c         1213 g          792 t
ORIGIN      
        1 ggcttcccgc tgaccatcta cctcctctct ggctggctgg gcagccgggt gccgggactc
       61 gacctgctct cccataatgc gggccatctc tggtacacgg cactcggcct gagcggagat
      121 ccccacacga acgtcatcca cctcgcgagc tatgtgttca tcttcggcgg gctcctcctc
      181 ctcatgaacg cctggagcgt gctgtatgcc gcccggcggg atgaccggct agcggtgacg
      241 ggcccgtacg cctatgtcag gcatccccag tacctcgcct tcattctcgt gatgttcggc
      301 ttccttctcc agtggccgac ggtgcttacc atcatcatgt tccctatcct cgtctggagg
      361 tacgtttccc tcgcgagaga ggaggaggag agtgtgaagg ctgagttcgg aaatgagtac
      421 atggagtacc tggcccggac tccggccttc attccccggc tcttccggtc acacaccgtt
      481 cccccgcggg aagagccggg tatcccatga tgttttttca atgggtcaca gggattacgg
      541 acgatcgagg accctcggga ggctgagcca gaaccgtgat agacatcttc gccctcatcc
      601 tcctagtttc cttcctcgtc tatctcatcc cggggccggc aaggagattt gcggcgattg
      661 cgggatgggc ggccatcatt ctcagcctcc tagcagaggt gccggaatac ctctccctcg
      721 ataacctgct ctacccgctg ctggcgatag cctcggtgcc gttccttgtg atgaccgcgg
      781 aattcctcct tcgtgaggac ccccgggtga tgcggctcac ggacgccgcg gctatcgccg
      841 tgttcctctc ggcccccttt accctcgtgc ccgggctcgc ggatatcctc atcaccctcc
      901 tcatcgggca ggttgcatgg ctcctccatg ccctagggta ccatgcggac ttcctggcct
      961 ggaacatcat cgcgaggaac gggtttgcct ccgagatcat cctcgcgtgc actggtatca
     1021 cggcgattgc catcatgctg ggtgttgcag gatccgggcg gccgaccccg aggcagggcg
     1081 tcctcgcctt cctcctcgtt gtccccgcaa tctacctcct caacctgctc agggtcagct
     1141 tcgtattcat cgcctcctcg gagcagtggt tctcgttcct ccctgacctc accagtgatc
     1201 ctgcgcccgg gtacggggcc gtgagcttct tctgggccca taacgtcatc gccgagacgc
     1261 tctcggtcct ggctctcgtc gggatcgcct tcgggctgtt caggatcatc ccccagctcg
     1321 cggcatttgc acgggaagtg gttgacctgt atgcgggaga cattcgggca ttcatcggga
     1381 agacgggggc atagtattgc ccgatgtcga tatcgaagaa ggtatacagg aacatcaccc
     1441 ctgaggtttt cctcttccca acaggttatg tagatgaacg tagaatgcat caatcacgtt
     1501 gagccctttg aaatgggatt gcagacgagg ataaaggcgg attgctgaga ggatgtgatc
     1561 tatggcaatt gacccggtat gcggcatgga agttgacgaa gagcacgcga tcaagagagt
     1621 gatcggcggc aggacctact acttctgcat ggaaagttgt gctttgaggt tcgcggaccc
     1681 ggagcaggaa ctgaaaagca tgaagcggcg ggttgccgtc gcagtctccg gcgtgatggt
     1741 catcgcagcc ctccgcgtga tcgccaccct ctccctcgct gcgggtgcca cccttatcac
     1801 ctgggcaccg atcccccagc tcccattctt cacctggggc tactggctct ttctcctgac
     1861 aacaccggtt cagttcatcg gcggctggag tttctaccgg ggggcgtacg cagccctctc
     1921 ggcgcaccgg gcgaacatgg acgtcctcat cgcgatgggg acctcgacgg cgtacttctt
     1981 cagcgtcttc gtgctcttct ttcccggagt cctcccggtc gcgggaagag atgtgtactt
     2041 tgaggttgcg gcagtaatca tcgcctttgt tctcctcggg aaattcatgg aggattacat
     2101 gcggtcgagc acctctgcag cggtgcgcaa gctgatggat ctccgcccga gcacggcgcg
     2161 ggtgatacgg ggcggcaaag aggaggagat ccccgccgag atggtcatgg tcgacgaggt
     2221 tatcattgtc cggcccggtg agaagatccc gaccgatggc gtcgtggccg aagggagatc
     2281 cgcggtcgac gagaagatga tcaccggcga gagcatcccg gtcgagaagg ggccgggtga
     2341 cccggtcata ggcgcgaccc tcaacaaaat ggggatgatg aaggtccggg caacgaagat
     2401 cggctccgag acgacgctca tgcagatcgt ccggatggtc gaggaggcgc aggcatccag
     2461 tgcgccgatt cagcggctgg cggaccgggt cgccgaatac ttcgtccctg cggtcattgg
     2521 agtcgcggtc ctcaccttct ttacctgggc ggtgatcctc gggaacttca ccgcagggct
     2581 cctcgccttc gtcgcggtgc tcatcatctc ctgcccgtgc gccctcggga tcgccacgcc
     2641 cactgcactc atggtcggtg tcggaaaggg cgcggaggcc ggcatcctca tccggggagg
     2701 agagtacctc gagcgggcgc agagcctcac caccgttgtc tttgacaaga ccggcactct
     2761 tacaaagggc gagccttcgg tcacggacat cctcggcgag gaccagaacg aggtgatcag
     2821 gctcgcagcc atcgccgaga aggggtcgga gcacccgctc ggcgaggcga ttgtcagggg
     2881 cgcgaaggag cggggaatcg agctgaaaga ccccgaatcc ttcgaagcgg taccaggtca
     2941 cggtatcagg gtggttatcg gaggaaagaa cgtgctcgtc ggcaaccgca ggctgatgca
     3001 ggcaaacggg atcgataccg ccacctatga agagaagatc caggccctcg aggtccaggg
     3061 gaagaccgcg atgctcgtag cggtgaataa cgggatcacc ggtatcatcg ctgtcgccga
     3121 caccctgaag gagaactcgg tggaagcggt gaaggagctg aagagcctcg ggatcgagac
     3181 ggtcatgctc accggcgaca acgaacggac cgcccacgcc atcgcgcagc aggtggggat
     3241 cgagggggtg atcgcaaacg tccttcccgg cgagaaagcc gacgtgatca agaagctcca
     3301 gacagagaag aaggtcgtgg cgatggtcgg cgatggcatc aacgacgcgc cggcactcgc
     3361 gcagtccgac atagggatag cagtcgggag cgggtctgat gtggcgaagg agaccggcgg
     3421 gatcgtgctc atccgcgacg acctgcgcga tgtggtcgca ggcatcaagc tctccaaggc
     3481 gacgatgaga aagatcaggc agaacctctt ctgggcgctc gcctacaaca ccctcgccat
     3541 ccccgttgcg gccgtggggc tcctcaaccc tatcatcgct gcagcagcca tgtcgctctc
     3601 gtccatcacg gtggtgggga acgcggcgct cctgaggagg ttcaggttcg aggagaaggg
     3661 agcgtaatct cttaggggac gctcctctcc ctatttttca gtcaaccttg ctgttgtttc
     3721 tggaatctcc tctgcttcac acagccgggt gccaataggt cgctgtgcaa tgcgtgcaaa
     3781 acgttgccta tttcccgtcg gcccctgcga agtagttata gatcaccgcg accagggcac
     3841 cctcgacgag accacagatg agccaccaga aggcattaat cgcggcaccc agcggggtgg
     3901 gcgaagaacc cggatgaaaa ggtgcaatca ttccgacgaa atcaagtccc acaccggt
//
LOCUS       DATGDI010000192         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_136088, whole genome shotgun sequence.
ACCESSION   DATGDI010000192 DATGDI010000000
VERSION     DATGDI010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136088"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VKO45_07035"
     CDS             <1..873
                     /locus_tag="VKO45_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05665.1"
                     /translation="LTPGTVYHFRVVSADPFDRIYRSADFQFTTTGAAPTVTPTPGGG
                     GTGGGGGSGGGIHGILPGVCRATPGGVITPVTGIGTTTSCTIISSVNGMTDLRSSWSV
                     YIPEDPPAGATFITFIRSQPSDFQRSVFEAALASDGLQMTALAYVMEVAESLPVTRAS
                     ETIQMDVERSWVSQNGGVNAVRIISLGEDGLARILDTRFSNYALDTGHMNFRAIAHSD
                     SFLFALISVKAAPVIPVSPDITPTATVPTVTVPGTTSVPPPISLEVMAIALLLAAIIL
                     IVFGLVLNRRVKRR"
     gene            complement(995..2299)
                     /locus_tag="VKO45_07040"
     CDS             complement(995..2299)
                     /locus_tag="VKO45_07040"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMA05666.1"
                     /translation="MDMETASFQNLALSHELQRAIADLGFEEPTPIQTLAIPVIQSGK
                     DVIGQAHTGTGKTAAFGIPLLEKVDPHKRTIQALVLCPTRELAIQVSEELTRLAKYRK
                     GIIILPVYGGQSMDRQLSVLARGVQVVIATPGRLLDHIRRRTISLEQVQYVVLDEGDV
                     MLDMGFIEDIQAILARTPKGRQTVLFSATVPDGIRDLSRGYMRDPQTIRVVHENLTVP
                     GIEQRYYQVTEQEKLDALTRLLEEYNPELSLIFCNTKRRTDVLARRLRARGYLAEKLN
                     GDMTQGQRERVMAKFRKGEIGILVATDVAARGIDVEGIDAVFNYDVPNDPEYYVHRIG
                     RTARAGREGRSFTLVTGMDAGKIRDIQRYAKVRIGKPETEVRREPATITPPRIPEEIR
                     SRIEKGDLGEYRSKIQDWVNQGYSSLDIAAALLAGAKPPRSA"
BASE COUNT          541 a          839 c          827 g          574 t
ORIGIN      
        1 ctcaccccgg ggacggtcta ccatttccgg gtggtatcgg cagatccctt tgacaggatc
       61 tacaggagcg cagatttcca gttcacgaca accggagctg caccgaccgt cacgccgacc
      121 cccggtggcg gagggactgg cggcgggggc ggtagcgggg gcgggatcca cgggattctg
      181 cccggtgttt gccgggccac ccccggcggg gtcatcactc cggtgaccgg aatagggacc
      241 accaccagct gtaccattat ctcatctgtc aacggtatga cagacctcag gtcgtcatgg
      301 tcggtataca tccctgagga tcccccggca ggggccacgt tcataacctt tatccggtca
      361 caaccatctg atttccaaag gtccgtattc gaggctgcgc ttgcatcgga tgggctccag
      421 atgaccgccc ttgcctacgt gatggaggtg gcggaatccc tgcctgttac gagggcgagt
      481 gaaaccatcc agatggatgt ggaacgatcc tgggtcagcc agaacggcgg cgtcaatgcc
      541 gtgcggatca tcagccttgg cgaggatgga ctcgcccgta tcctggacac gaggttctcc
      601 aattatgccc tggatacggg tcatatgaac ttcagagcga tcgcgcattc cgactccttc
      661 ctgttcgccc tcatctccgt gaaggccgcc cccgtaatcc cggtctcccc cgatataacg
      721 ccgacagcca cggtaccaac agtgacggtt cccgggacca cctcagttcc acctccaata
      781 tccctggagg ttatggcgat cgccctcctc cttgctgcca tcatcttgat tgtcttcggg
      841 ctggttctca acaggcgggt aaagaggaga tgaaaatgcc ctgcatgggc ctgccccgaa
      901 gtgctccccc tgggaattcc aatttaccaa attccttatc gcagaaggcc agaggttccc
      961 tgttttgcag ggcagaatgg ggggtgccga ctccttaggc agaccgggga ggctttgcac
     1021 ctgcgagcag ggcggcggcg atatcaaggg agctgtaccc ctgattcacc cagtcctgga
     1081 ttttcgaccg gtactcaccg agatccccct tctcgatcct cgagcggatc tcctcaggaa
     1141 tccggggagg agttatggtc gccggttctc tccggacttc cgtttccggc ttcccgatac
     1201 gaaccttcgc ataccgctgg atatcccgga tcttcccggc atccatgccg gtcacgaggg
     1261 tgaaggatcg tccctcccgg cccgcacggg cggtgcggcc gatccggtgg acataatact
     1321 cggggtcgtt gggaacgtcg tagttgaaga ccgcgtcgat gccctccacg tcgattcccc
     1381 gcgctgcaac atcggtggcc acgaggatgc cgatctcccc cttccggaac ttcgccatca
     1441 cccgttcccg ctggccctgg gtcatatctc cgttcagctt ctcggccagg tacccccggg
     1501 cccgcagccg ccgtgccagg acgtccgtcc ggcgcttcgt gttgcagaag atgagggaga
     1561 gctccgggtt gtactcctcg aggagcctcg tcagggcatc gagcttctcc tgctctgtca
     1621 cctggtagta ccgctgctcg atgcccggaa ccgtgaggtt ttcgtgaacg acccggatgg
     1681 tctgcgggtc cctcatatac ccacgggaca gatcgcggat cccgtccggg acggtcgccg
     1741 agaagaggac cgtctggcgc cccttcggcg tccgggcgag gatggcctgg atatcctcga
     1801 tgaagcccat gtcgagcatc acgtccccct cgtcgaggac cacgtactgc acctgctcca
     1861 gggagatggt tcggcggcgg atgtggtcga ggagccgtcc cggggtcgcg atgaccacct
     1921 ggactccccg ggcaaggacc gagagctgcc gatccatgga ctggccgcca tagaccggga
     1981 ggataatgat ccccttccgg tacttcgcga gcctggtcag ctcctccgag acctggatgg
     2041 caagctcgcg ggtggggcag agcacgaggg cctggatggt gcgcttatgg ggatcgacct
     2101 tttcaaggag ggggatgcca aaggctgcgg tctttccggt cccggtgtgg gcctggccga
     2161 taacgtcttt cccggattgg atcaccggga tggcgagggt ctggatcggg gtcggctcct
     2221 cgaacccgag gtcggcgatg gcccgctgca gctcgtggga gagggccaga ttctgaaagg
     2281 atgctgtttc catgtccatc aatccaggga acccgatgca ttccctgccc tgatgacgat
     2341 tcatcgaaaa gaggatgacc ggtccgctgt gtgtggcaat acattgttgc tcttttcata
     2401 aagggttatg ggagactctg gctgcccttt ttccatcatc ctgccatggg attctccctg
     2461 ttcaatggta tcttacgggc gatggctccc gcatcccgtc agaaaagacc aaaagaaaat
     2521 atgaagatag atgcgactcg aggagaaaac cagtctgaac acaggtcaca agtatcatca
     2581 ggacatactg ccatttccta tcccccgaca gccttttccc ataggcgtac tggagagaac
     2641 cgcaaaggag agcgatgagg agagctcccg atagcagtat tacaggactc ccgttcccga
     2701 atatcaagaa aggcgcccat attatggagg acatgaccgg tatgaagaag attgaaggca
     2761 tttgcatgag gagaaccgga a
//
LOCUS       DATGDI010000193        20344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_368678, whole genome shotgun sequence.
ACCESSION   DATGDI010000193 DATGDI010000000
VERSION     DATGDI010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 20344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20344
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368678"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /gene="ribC"
                     /locus_tag="VKO45_07045"
     CDS             <1..428
                     /gene="ribC"
                     /locus_tag="VKO45_07045"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HMA05667.1"
                     /translation="VDMGKFAVNELRKHASVSIERYTVPGIKDLPVACKILIEERGCD
                     LVMALGMPGAKELDRVCAHEASQGIILAQLMTNRHVIEVFVHEDEAEDGRELAWLAEQ
                     RTREHAVNAVKLLAAPRALAKEAGTGQRQGFPDAGPARE"
     gene            479..886
                     /gene="ribH"
                     /locus_tag="VKO45_07050"
     CDS             479..886
                     /gene="ribH"
                     /locus_tag="VKO45_07050"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HMA05668.1"
                     /translation="MAVKLGFVVGEFNRDITYMMEVEGREHAKFLGAEVAETIYVPGT
                     FDVPLAVKKMLSKGRVDAVVTLGAVIEGATQHDEVVIQHAARKIMDLGLEFGKPVTLG
                     ISGPGMTRLEAQERIDYAKRAVESAVKLVQRLG"
     gene            883..2040
                     /locus_tag="VKO45_07055"
     CDS             883..2040
                     /locus_tag="VKO45_07055"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMA05669.1"
                     /translation="MKALSSKVAGIASSATLDIADRAKAMAREGIDVISLSIGEPDFD
                     TPVHIRDACCESLRRGDTHYAPSRGIPELLQAIAGKIQSENGFSCSPEQVIATAGAKH
                     AIAMAMEACLNPNDEVILLDPAWVSYEPCVQLAGGRVVHQKVNPKTFLPDDTLLDRIT
                     GRTKMIVVNSPNNPTGAVFDKKALKLIADVCTDYELLAMSDEIYEKLIYGKEHVSLAS
                     VGDMAGRTITVNGFSKAYAMTGWRLGYAVAPVEVIRQMNKIQQHGVSHPTTFVMWAGV
                     AALKGDQSCVAEMKKEFESRRMFLMDEFSELKVQAAPADGAFYAFINARGDDVEVAEH
                     WLNKARVAVTPGTAFYAPGWIRISYATAMPRLREAIGRIKTVWNEAPERKR"
     gene            complement(2094..2870)
                     /locus_tag="VKO45_07060"
     CDS             complement(2094..2870)
                     /locus_tag="VKO45_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HMA05670.1"
                     /translation="MTLTIPQVGARLAEAGRLTSRPLCIAGSDTVPRGAAPAPSVDRC
                     LAKAIAAVAINPNTPPIYLGPGALEGCCPSGIAYTGFGEQTRDARFFISTGSPGVAGG
                     MGLNLKAGPDLVDASRLAVGEIRPIWEYLVVRPAENYPSDAHEPRSMLLFAQAEPARV
                     LCALAHFGSPDVFGAVLAPWGSACASFVTYPAGLAEKAPKNAIFLGPTDASVNLWFPR
                     EMIALGIPMDIARKMAEEVPASFIGKRPGMAYPERRVYLK"
     gene            complement(2953..3291)
                     /locus_tag="VKO45_07065"
     CDS             complement(2953..3291)
                     /locus_tag="VKO45_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMA05671.1"
                     /translation="MKITEVAKVVSGPNPHHVDARKIYDTPHAMAVVITLKPGEFLKR
                     HMTPVDVFFYVLEGEGVVEIGDERKTVGKDVLVESPARIPHRWMNEGRGTFRVLVVKV
                     PKPTEETKLL"
     gene            complement(3288..3983)
                     /locus_tag="VKO45_07070"
     CDS             complement(3288..3983)
                     /locus_tag="VKO45_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMA05672.1"
                     /translation="MTVSIAGATLQRDGKTYAVSTRTPGGLITPDHLERVAAIARKYG
                     IPLMKITSGQRIALVGVQESQLEGLFRDLDADADRTGGPCLHFVQACLGTESCRFGNQ
                     DSFGLARAVEARFREKVYPAKLKIGVSGCPRSCGESHTRDVGILGSAGGWTIIFGGNG
                     GTRPRIGDVVARNLPMEEALDLVERLLEYYRIHANPKERTARFMERIGMETLKSELLA
                     FVPFLPLEKVKRT"
     gene            complement(3984..4847)
                     /locus_tag="VKO45_07075"
     CDS             complement(3984..4847)
                     /locus_tag="VKO45_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMA05673.1"
                     /translation="MDLIANLMQLGLKEYEARIYVALAGLGEANVRKLHEASGVPRPR
                     VYDILAALATRGFIDVRQGSPLTYTAVRPERVISSLKKELDDAAEESRKALAALSVDA
                     RTHYSPIWYLQSDRTIQRYLEMLVDGVSEELVILCFNRETLRRFQAPVARIAGSRTVK
                     ILFPKGFADDSGPADGIGYYEAGKLRNLFGMNIFEKVFSVPISREGSVFRLECILLAD
                     DRESMLIYTLDGNRMAVTITLPFITAVQHQLFRQLIARAREVRPGRRPAAPLRKGKRG
                     PERSRMAVEVG"
     gene            4903..5343
                     /locus_tag="VKO45_07080"
     CDS             4903..5343
                     /locus_tag="VKO45_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HMA05674.1"
                     /translation="MTPISEERENMQVVRRFFEVGPSKGDLEAADALLAPEFSLHTPL
                     PTPGPGIEAMNTVITTCRAAFHGLSVTVHDIMAEGDKVTCRFTARGVHKGEFMGISPT
                     GKGIEMTGIEIFRLRDGKIVELWGEANLMGLMQQLGILSGPPQD"
     gene            5347..5655
                     /locus_tag="VKO45_07085"
     CDS             5347..5655
                     /locus_tag="VKO45_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAC family transcription factor"
                     /protein_id="HMA05675.1"
                     /translation="MPGDGKDGYYCTICGGIPPDQVKIKKILIDGKETGIDRIDWILL
                     DVMALGLDDEEKITDEIVKRVRRFNFIPSLKEAEYRKALLREYLMLAASGVQPEKKNG
                     "
     gene            complement(5662..6582)
                     /locus_tag="VKO45_07090"
     CDS             complement(5662..6582)
                     /locus_tag="VKO45_07090"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HMA05676.1"
                     /translation="MVKLSHIAAAIILAAAIIAAGCTTLGQTSSPTMESVSKSQGGLT
                     YGGVVPAPTVAPASRLATDQAAGGTGTDQGFQTKIIKTGQVTIEVAQVPAAIDQIRNL
                     AQSNGGYLSTSSVYTSQNDRKTGYAVIRIPADRFDTVMQALAPLGKILSSSEQRSDVT
                     EQYVDLTIQNESYHTQLDNYYRLMAKATDVEDIIKIQAQIDQITLSINQVEGRLRYLS
                     SQIDLSTITVNLQEPEPVGGETGFNIITAINEGIAGFFGMIGAIIVLVFSLLPLIILV
                     IIAYAVYRYYKKRKGGSATPAAAAPAGTKA"
     gene            6838..7434
                     /locus_tag="VKO45_07095"
     CDS             6838..7434
                     /locus_tag="VKO45_07095"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HMA05677.1"
                     /translation="MQRIPLAVILLALLLSLSAGCTKEGPQTGSLEITSTPADREVQV
                     ILDGNFQGVTPLLLTNLPAGSHLLQLRSTGYAEKVELVTVSAGQKMIFSATYPPLPTL
                     TQVTPTAFPATPVPTTAILTLPTTPLLPGSLYVTSFPSGATIYLNGRGYGITPRLIQN
                     LTPGSYELRLSLVGWDDYQMPVSVSPGLTEEEHAILRS"
     gene            7450..7932
                     /locus_tag="VKO45_07100"
     CDS             7450..7932
                     /locus_tag="VKO45_07100"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HMA05678.1"
                     /translation="MADRRKVLFVCGRNAARSQMAEGFLRALYGDLYEAASAGLRPSV
                     VSSIAIRVMGEAGIDISSHRSKSLDEFSGEHLDLVVTLCDDASCVRDALLPDAGRYLH
                     RVFPDPAAFAVSEEEVLAAHRDVRDRIGEWIREEFGPGRTVRGSGRTPLPPPPDLQKT
                     "
     gene            8034..9161
                     /locus_tag="VKO45_07105"
     CDS             8034..9161
                     /locus_tag="VKO45_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05679.1"
                     /translation="MNESTLLLSVALILLLAVPASALDSGPQVGVSSYTLDPPALMKG
                     DTGIVTIHVVNSGTDASYINSARLSGGEVTVIQSPDQTQGGIAPGSGMTYVYKVQAVG
                     PDGVYYLKFILDFREGGQYSFNIPVEIESSTLKLSILDMPDTTIQGVRGEYRIMVGNP
                     RPDAVNAVTITPEGDGFLVTPSGVFLGTLGADASSVVTFNVTPLRQTFIRFRANYKNG
                     INPQTTTLTLPLSTVENRLAAEPVLTNIHVTAEQGAIHVSGDVTNVGLETARSVTITT
                     QGTVTPADPYKVFVVGSLEPDDISSFEVTFRSKADITEVPLLVTYKDPAGNVITATSN
                     VEISSQALSDQASEPSRFTNLLIWMFVVLVAAVFIYFWRRG"
     gene            9170..10351
                     /locus_tag="VKO45_07110"
     CDS             9170..10351
                     /locus_tag="VKO45_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA05680.1"
                     /translation="MTGARPGPFATAARDIREHWVRSLFAIVGIIIGVAAISTLGMLG
                     NGLALSVSDSMRYAGDTIVITPHVSASPGSAGGEGSGLMITEHQVGEIRRAAASNPVI
                     PIRTGAERITVGSGAESVAVYAMAPADVPVLLEKASGNYLRYGSGVMAGKGLADATGL
                     SVGDTIGIGAGEERVRVTGILKERGIGFDLSPDDALIVPDTWFAARYGVHDYDLVIIR
                     VKNPDDIDAVKAAVDEQLNLRDPVVNILDTRRVLTTIVDSFNRVSAAIMTAGGISLAV
                     AGVSLLSVMLMSVTERAGEIGMIRSLGATRSEVMRIFLSEALILGLAGSAAGGILSLA
                     GGYLASGGMLQTTKFLLVPATIVPILTGMGFGISVSLLAGIYPAWKAAMMSPAGAPRA
                     G"
     gene            10561..11328
                     /locus_tag="VKO45_07115"
     CDS             10561..11328
                     /locus_tag="VKO45_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05681.1"
                     /translation="MILGYAEDAMEPFISQDGQILFFNSLNDGKDTSLYLARRSGDAT
                     FQLVGKVGGANGPPPHLDAVASMDAANRFYFVSTRDYPAADENLFVGSFRDGAITGVR
                     PVQGDFYLRQPGWIVMDAEIHRGGEVLLYVDAHFTGGAMPDAAFIGAARQVNGTFATD
                     PNNGILFREVNTGDLNYAPSLSADGLELFFTRAAGTTTRLLVSRRDSLTEPFGAPQEL
                     AIRGEAVEAPCISLDGRFLYFHMREGGKFAIFVASRN"
     gene            11526..12884
                     /locus_tag="VKO45_07120"
     CDS             11526..12884
                     /locus_tag="VKO45_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HMA05682.1"
                     /translation="MQSKFQLDEDQIPKRWYNIQADLPSPMDPYYSSQTMKLATPEEL
                     HQIFPMALIGQEVSQDRYIDIPEEVREIYRLYRPSPLYRAHRLEAHLKTPAKIYFKYE
                     GVSPPGSHKPNTAIPQAYYNMKEGIQRISTETGAGQWGSALAFATALFGMKCTVYMVR
                     SSFYEKPYRKSMMQAWGAECIPSPSDRTQSGKAMLAKDPKTPGSLGIAISEAVEDAAT
                     HKDTNYSLGSVLNHVCMHQTIIGQEAMEQLKMAGDEPDMIIGCVGGGSNMAGISFPFL
                     GEKLRGKRKDLEAIAVEPAACPTLTKGVYAYDYGDTVGIGPIAKMYTLGHDFVPPAIH
                     AGGLRYHGDSPLISRLVHDKVMRAVAVHQTEVFQAAVTFARTEGIIIAPETAHAVKTV
                     IDEALRCRENAEEKVILFNLSGHGNLDLGAYDEYFEGKLSDFEYPDSKIKESLAHLPK
                     VG"
     gene            13019..13936
                     /locus_tag="VKO45_07125"
     CDS             13019..13936
                     /locus_tag="VKO45_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HMA05683.1"
                     /translation="MLLRYAGCLLGAAIGDAMGMPGEGTPADLWRAECSYRRAYRGHP
                     NEALAPGQFTDDTQLLLLSAGLLADRAFTPEKYAERLAERYRSGDLRFPDGSVVAACL
                     HLMEGTASDRSGVNSTTAGCIPAGIPFALAYRDPVEIRETVARVTSITHTNPAAIAGC
                     TGIALLIHHTLAGSPDDLDIAWGEVSREDVLLGEKIRHALYLEREGIGLEHAIRLIGN
                     DLSVYQTLPLAFFLMARCSNAAELLSLVGHVGGNTSTIGLICGAYLGAKYGMNVLPRQ
                     LIEGLEDRARIETLAARLLEVSSDIPRIP"
     gene            14068..15063
                     /gene="argC"
                     /locus_tag="VKO45_07130"
     CDS             14068..15063
                     /gene="argC"
                     /locus_tag="VKO45_07130"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HMA05684.1"
                     /translation="MNHSQIAIIGASGYTGGDLIRLLLSHSSAEVTIATSRKLVGKRV
                     DSVHPNLKGLTDLVFENPPVDRMDADVALLAVPHTAAMGYVRGLLDRGMKVVDLSADY
                     RLPKEVYEETYGVPHTDYFPAPYGLTELHRDEVKGVSFVSNPGCFPTGATLAAAPLAK
                     MAKAVVFDSKTGVSGAGNSPSAVTHYPTVADNINPYKWTRHRHLAEMKQEMARLGTKG
                     GVYFTPHLVPVNRGILTTAHILLSEPMEQKEVEAMYRKFYEKEFFVRLQQPILAAVRG
                     TNFCDIAVESEGERVVAVSAIDNLVKGASGQAIQNMNLMCGFSEQDGLRGAGVLP"
     gene            15072..15572
                     /locus_tag="VKO45_07135"
     CDS             15072..15572
                     /locus_tag="VKO45_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA05685.1"
                     /translation="MKVRDVMTPDPVVVSADATLREAAGLLRKNRIGGLPVMDGERLA
                     GMITESDILRLLKTPGVSGDLWLPSPLEIIEVPIREAINWAHTQKALSHIGDQPVRSV
                     MSSPAITIGEREDMDAAAALMVKKRIGRLPVVRGRKLVGILARADIVRGLGESYGGTK
                     SGEEAP"
     gene            15569..16720
                     /gene="argJ"
                     /locus_tag="VKO45_07140"
     CDS             15569..16720
                     /gene="argJ"
                     /locus_tag="VKO45_07140"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HMA05686.1"
                     /translation="MKSICGVEGVTAAGMKEGRYGLALVAASGTAAAVFTSNLVKAAP
                     LILMQERIARGELDGIIVNSGCANAFTGERGLRDAALLAEIGAGSFRTDPERVGVAST
                     GIIGRYLDLDLIRRQAGEVSPRMRRDPAAEVEAARAIMTTDLVEKHALIPGDGFSVGG
                     ICKGSGMIAPNMGTMIALLYTDAEVPAGSLRGALRTATRRSFNQVVVDGDTSTNDIAF
                     LTATGEAGKVRPAALGKALEECCRSLAIQIARDGEGATKLIEIVVRGARTEKGASLVA
                     RTVVGSPLLKTAVYGEDPNWGRVLAAAGRAGVEFDPDRVSLTIGEGEGRTPLVQGGRI
                     VVDLARAKQAMKGKEVRFELDLGEGNREATAWGCDLTEKYVEINGRYTT"
     gene            16717..17586
                     /gene="argB"
                     /locus_tag="VKO45_07145"
     CDS             16717..17586
                     /gene="argB"
                     /locus_tag="VKO45_07145"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HMA05687.1"
                     /translation="MKREEVLMEALPYIQRFHGKTIVIKLGGHAMVDQEILEMAIRDA
                     VLLQYVGMKVVIVHGGGPEITEKMQAMGKEPKFVGGLRVTDAETLEIAQMVLVGKIND
                     GIVSLIAKGGARGVGLSGNDGNLMIARKMDPQKVKEGNRVKKVDLGFVGEIEEVDPTL
                     LNTLLDRGYIPVIAPIAIDREGTSLNINADTAAGEIAVALGAFKLVNLTDVDGVMDSG
                     RTQVFRRLTTQELEKLIADGTVSGGMIPKITSSLQSVKRGVGQAHIINGNQGHNLLLE
                     LFTDAGVGTMISR"
     gene            17670..18233
                     /locus_tag="VKO45_07150"
     CDS             17670..18233
                     /locus_tag="VKO45_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HMA05688.1"
                     /translation="MDIFSIIILVIVVIVILALIFWFVGIYNQFYKYRNAADATLGQI
                     RVAMKKRLDMIEQLLGAVKSYAKFEKETLTKITEMRASVLKGTPSELNTVERESRALL
                     GTLIATAEAYPDLKTSSTVTSLMDSVKGVEEEIARQRYTYNNIVQQFNTMLDVLPSSF
                     IGRLIGLLKLDYLQFEEAISTPPKIEF"
     gene            18258..20075
                     /locus_tag="VKO45_07155"
     CDS             18258..20075
                     /locus_tag="VKO45_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HMA05689.1"
                     /translation="MSENRQIATLMAITLVIGVIALALSLALPGALEGNLVIDRYTAS
                     VSADGTLSEHYDYVVKDPGVYRFLFRTWEVPLAGSALPQPSVEVIGIKVPAGTAGYYR
                     DYRGQVTIYGITTAEERSTIQSLVQDNEIGFYTPSYFPAGTYSVDFTYRIHPPIEYDP
                     RWSHLNLRFADQHVPYHAIQVSIPSAGVESVYPYPPFLAVMQSGGTTTISGSAAANEV
                     VGVEIVASRDYTVNWQGFPDAVSDVQGLAESAFFWYSLPYYAGALLLAIAGILVLIMP
                     ALLYIVYNRYGREKEFTVPEYLSFVPDRTLPPWVVNLVFKDEALTFDENGFYATVLDL
                     GRKKFLEIKPKGESAEAGVTIRVLQGTSPDPYEQRVLNFLRNLADGGVVDTDALKGLT
                     ESARADRSSELRAMKIQATLRGITTEVDPTVANRFVIDGRPHLVPLLLGSAILAGLSI
                     LILVIGSQAVTLLLPAAVLGSVAVIQVAIAWAFPSTLFGQWKGDFYQEKLQWDAFRKF
                     LSDLARIKEYAPQDIAMWGEWLVYGTALGVGDNVTKAMKELNIRVPEMYVVPALYLGF
                     APIWGFAPPSRGGMGGGGFHGGGGFGGGGGFGGGGAGGR"
BASE COUNT         3646 a         6613 c         6549 g         3536 t
ORIGIN      
        1 gggtagacat ggggaagttc gcggtgaacg agctccggaa gcacgcctcc gtctccatcg
       61 agcggtacac ggtcccgggg atcaaggacc tccccgtcgc ctgcaagatc cttatcgagg
      121 agcggggctg cgacctcgtc atggccctcg gcatgccggg cgcgaaggag ttggaccggg
      181 tctgtgccca cgaggcatcg cagggcatca tcctcgccca gctcatgacg aaccggcacg
      241 tcatcgaggt cttcgtccac gaggacgagg cggaggacgg gcgtgagctc gcctggctcg
      301 ccgagcagag gacccgggag catgcggtga acgcggtgaa gctcctggcg gccccccggg
      361 ccctcgcgaa ggaggccggg accgggcagc ggcaggggtt ccctgacgcc ggcccggccc
      421 gggagtgagg aaagggtcgg tttatatggc aaagagacga gatcagatgc gggataccat
      481 ggctgtgaag ctcgggttcg tcgtgggtga gttcaaccgc gacatcacct acatgatgga
      541 ggtcgaggga agggagcatg cgaagttcct cggggccgag gtggccgaga ccatctacgt
      601 gccggggacc ttcgacgtcc cccttgccgt gaagaagatg ctctcgaagg ggagggtgga
      661 cgcggtggtc acccttggtg cggtcatcga gggtgccacc cagcacgacg aggtcgtcat
      721 ccagcacgcg gctcggaaga tcatggacct cggtctcgag ttcgggaagc cggtcaccct
      781 cgggatctcc gggccgggga tgacccggct cgaggcccag gagcggatcg actacgcgaa
      841 gcgggccgtc gagtctgcgg tgaagctcgt ccagcgattg ggatgaaggc cctctccagc
      901 aaggtggcgg ggatcgcctc ctcggccacc ctggacatcg ccgaccgtgc gaaggcgatg
      961 gcccgggagg ggatcgacgt catcagcctc tccatcgggg agcccgactt cgataccccg
     1021 gtccacatcc gggacgcctg ctgcgagtcc ctccgccggg gggacaccca ctacgccccg
     1081 agccggggca tccccgaact cctccaggcc atcgcgggaa agatccagtc tgaaaacggt
     1141 ttttcctgct cccccgagca ggtcatcgcg acggccgggg cgaagcatgc catcgccatg
     1201 gccatggagg cctgcctgaa cccgaacgac gaggtgatcc tcctcgaccc ggcctgggtc
     1261 tcgtacgagc cctgcgtcca gctcgccggg ggaagggtcg ttcaccagaa ggtgaacccg
     1321 aagacctttc ttcccgacga caccctcctg gaccggatca ccggccggac gaagatgatc
     1381 gtggtgaact ccccgaacaa cccgacgggt gcggtctttg acaaaaaggc cctcaagctc
     1441 atcgccgacg tctgcaccga ctacgagctc ctcgccatgt ccgacgaaat ctacgagaag
     1501 ctcatctacg ggaaggagca cgtctccctc gcctccgtcg gtgatatggc gggccggacc
     1561 atcacggtga acggcttctc caaggcctat gccatgaccg gctggcggct cggctacgcg
     1621 gtcgcgcccg tcgaggtcat ccggcagatg aacaagatcc agcagcacgg tgtctcccac
     1681 cccaccacct tcgtcatgtg ggccggtgtc gcggccctga aaggggacca gtcctgcgtc
     1741 gcggagatga agaaggagtt cgagagccgg aggatgttcc tcatggacga gttcagcgag
     1801 ctgaaggtcc aggccgcccc ggccgacggg gccttctatg ccttcatcaa cgcccggggg
     1861 gacgatgtcg aggtcgccga gcactggctc aacaaggcga gggtggcggt cacccccggg
     1921 acggccttct atgccccggg atggatccgg atctcctatg ccacggccat gccccggctt
     1981 cgggaggcca taggccgaat caagacggtg tggaacgagg cgccggagcg gaagaggtag
     2041 gggcgcgggc ccgtcctctg tccttttacc aaaccctcga tcgcttttcc gttttacttc
     2101 aggtacaccc gtctctccgg gtatgccatc ccgggccgct tcccgatgaa ggaggctggc
     2161 acctcctcgg ccattttcct cgcgatgtcc atggggatcc cgagggcgat catctccctc
     2221 gggaaccaga ggttcaccga ggcatcggtg ggcccgagaa agatcgcgtt cttgggtgcc
     2281 ttctccgcga gaccagccgg ataggtgaca aagctcgcgc atgccgaccc ccacggggcg
     2341 aggaccgccc cgaacacgtc ggggctcccg aagtgggcga gggcgcagag gacccgggcg
     2401 ggctcggcct gggcgaagag gagcatggac cggggctcat gggcgtccga agggtagttc
     2461 tccgccggcc ggaccacgag gtactcccag atcggccgga tctcccctac cgcgagccgg
     2521 ctcgcatcca cgagatcggg gcctgccttc aggttcaggc ccatcccgcc ggcgaccccc
     2581 ggggaacccg tggagatgaa gaaccgtgca tcccgggtct gctcgccaaa ccccgtgtag
     2641 gcgatacccg aggggcagca tccctcgagc gcacccggcc cgaggtagat gggcggtgtg
     2701 tttgggttga tggccacggc cgcgatggcc ttcgccaggc agcggtcaac cgagggagcc
     2761 ggcgcggcgc cacggggaac ggtgtccgag cccgcgatgc agaggggccg gctcgtgagc
     2821 ctccccgcct ccgcgagcct tgccccgacc tgggggatgg tgagcgtcat gatgatcctc
     2881 aggggaggat tgaacccgga gaggatgagg gtttgggtgg cgggatcaac ccaaaaaggg
     2941 ggggtgacca gatcagagga gcttcgtctc ttcggtcggc ttcggcacct tcaccacgag
     3001 gactcggaag gtgcctctcc cctcgttcat ccaccggtgg ggaatccggg cagggctttc
     3061 cacgagaaca tcctttccca ccgtcttccg ttcatccccg atctcgacga ccccctcgcc
     3121 ttcgaggacg tagaagaaca catccacggg cgtcatatgc cttttcagga actcacccgg
     3181 tttgagagtg atgacgaccg ccatcgcgtg gggcgtatcg tagatcttcc gtgcatccac
     3241 gtggtgcggg ttcgggcccg acaccacctt tgccacttcg gtaatcttca tgttcgcttc
     3301 accttctcga gggggaggaa ggggacgaag gcaaggagct cgctcttcag ggtttccatg
     3361 ccgatccttt ccatgaacct cgcggtcctc tccttcgggt tcgcatggat gcgatagtac
     3421 tcgaggaggc gctccacgag gtcgagggcc tcttccatgg ggaggttcct cgcgacgaca
     3481 tccccgatgc ggggccgggt cccgccgttc ccgccgaaga tgatcgtcca tcctccggcc
     3541 gaccccagga tcccgacgtc ccgggtatgg ctctctccgc aggaacgggg acagccggac
     3601 accccgatct tcagcttcgc cgggtagacc ttctccctga accgggcctc gacggcccgg
     3661 gcgaggccga aggagtcctg gttcccgaac cggcagctct cggttcccag gcaggcctgg
     3721 acgaagtgga gacaggggcc tcccgtccgg tcggcatctg catcgagatc cctgaagagc
     3781 ccttcgagct gcgattcctg caccccgacg agggcgatcc gctgtcccga cgtgatcttc
     3841 atgaggggaa tgccgtactt ccgggcgatc gcggccaccc gttcgaggtg gtcaggggtg
     3901 atgagccccc cgggcgtcct tgtactcacc gcatacgttt ttccgtcgcg ctggagggtg
     3961 gctccagcaa tgcttaccgt catctatccg acctccactg ccatccggct ccgctccggc
     4021 cctcttttac ctttccggag gggggccgcg ggacgcctcc ccggccgcac ctcccgggcc
     4081 ctcgcgatca gctgcctgaa gagctggtgc tgcacggcgg tgataaaagg gagggtgatg
     4141 gtaacggcca tccggtttcc gtccagggtg tagatgagca tggactcccg gtcgtcggcg
     4201 aggaggatgc actcgagcct gaagaccgac ccctcccggc tgatgggaac cgagaagacc
     4261 ttttcaaaga tgttcatccc gaagaggttc ctgagttttc cggcctcgta gtacccgata
     4321 ccgtctgcgg ggccggaatc gtcggcgaat cctttcggga agaggatttt cacggtgcgg
     4381 gatccggcaa tccgggcgac gggtgcctgg aatctcctca gggtctcgcg gttgaaacag
     4441 aggatgacga gctcctcaga caccccgtcg acgagcatct ccaggtaccg ctgaatggtc
     4501 cggtcgctct gcaggtacca gatgggagaa tagtgtgtgc gggcatcgac ggaaagggcc
     4561 gcgagggcct tcctgctctc ctccgcggcg tcgtcgagtt cctttttcag ggacgagatc
     4621 acccgctccg gccggacggc ggtgtaggtg agcgggctcc cctgccttac gtcgataaat
     4681 ccccgggtcg cgagggccgc gaggatgtcg tagacccggg ggcggggaac cccgctcgcc
     4741 tcgtggagtt tccggacgtt cgcctccccg aggcccgcga gggcgacgta gatcctcgcc
     4801 tcgtactcct tcagcccgag ctgcatcagg ttcgcgatca aatccatttg ttactctata
     4861 agagacaaca acattatata ggtatccgtc cacggatccg gtatgacacc gatatctgag
     4921 gaacgggaga acatgcaggt ggttcgcagg ttcttcgagg tgggcccatc gaaaggagac
     4981 cttgaggcgg ccgatgcgct ccttgccccg gagttttcac tgcacacccc cctgccgacc
     5041 ccgggcccgg gcatcgaggc gatgaacacg gtcatcacca cctgccgggc ggcctttcac
     5101 ggccttagcg tgaccgtcca tgacataatg gccgagggcg acaaagtcac ctgccggttc
     5161 accgcccgcg gagtgcacaa aggggagttt atggggattt ccccgactgg gaaggggatc
     5221 gagatgaccg gtatcgagat cttccgtctt cgcgatggca agatagtaga gctctggggc
     5281 gaggcgaacc tcatgggcct catgcagcag ctcggtatat tgtccgggcc tccccaggat
     5341 tgatcgatgc caggggacgg gaaggacggg tactattgca ccatctgcgg aggcatcccg
     5401 cccgaccagg tcaagatcaa gaagatcctt atcgacggga aagagaccgg tatcgaccgg
     5461 atcgactgga tcctcctcga cgtgatggcc ctcggcctcg atgacgagga aaagatcacc
     5521 gacgagatcg tgaaacgggt gaggaggttc aactttatcc cgagcctgaa ggaggcggag
     5581 taccggaagg ctcttctcag ggaatacctg atgctcgccg cgagcggtgt tcagccggaa
     5641 aaaaagaatg gatgaggaga ctcacgcctt cgttcccgcg ggggctgctg ccgccggggt
     5701 ggcgctgccg cccttccgct tcttgtagta ccggtacacc gcgtacgcga tgatcacgag
     5761 gatgatcagg gggagaaggg agaagacgag gacgatgata gccccgatca ttccgaagaa
     5821 gcccgcaatc ccctcgttga tggcggtgat gatattgaac ccggtctcgc cgcccacggg
     5881 ctcgggctcc tggaggttca cggtaatggt ggagaggtcg atctggctgc tcaggtaccg
     5941 gagccgtccc tcgacctggt tgatggagag cgtgatctgg tcgatctggg cctggatctt
     6001 gatgatgtcc tcaacgtcgg tggccttcgc catgaggcgg tagtagttgt cgagctgggt
     6061 atggtaggac tcattctgga tggtgagatc cacgtactgc tcggtcacat cagaccgctg
     6121 ctccgagctg gagaggatct tacccagggg tgcgagcgcc tgcatcaccg tgtcgaaccg
     6181 gtcggcgggg atccggatca ccgcataccc ggtcttccgg tcgttctggg aggtgtagac
     6241 actggaggtg gagaggtatc ccccgttgga ctgggcaagg ttccggatct ggtcaatggc
     6301 tgccgggacc tgggcgacct cgatggtgac ctggccggtc ttgatgatct tcgtctggaa
     6361 tccctggtca gtgccggtcc cgccggcagc ctggtcggtg gccagtctcg aggccggtgc
     6421 tacggtgggt gcggggacca ctccgccgta ggtgaggccg ccctggcttt tcgagaccga
     6481 ttccatggtc ggggatgagg tctggccgag ggtcgtgcat ccggcggcga tgatggccgc
     6541 ggcgaggatg atggccgcgg cgatgtgcga aagttttacc atggatgagg gtaagccggc
     6601 gggaaataaa gaatttctgt agattacgaa tattttcgtt gcgatgagaa aatgcgccgg
     6661 cattttcgaa accatcgaaa atcccgaagg cggttcttga tcccgttgaa attccggatg
     6721 gaaatttccg aggcggaatt ccctcgcttc ccggatcagg tttccgcctc cgggacacca
     6781 ggttcgggcg tggacggtat ccataccttt aaacgctctg cctccggatt ttgtaccatg
     6841 cagaggatcc ccctcgccgt gatccttctt gccctgctcc tctccctctc cgcggggtgc
     6901 acgaaggagg gaccgcagac gggatccctg gagatcacct ccacgccggc cgaccgggag
     6961 gtgcaggtca tcctcgacgg gaatttccag ggggtgaccc ccctcctcct caccaacctc
     7021 ccggcgggct cccacctcct gcagctccgg tccactggct atgccgagaa ggtcgagctt
     7081 gttaccgtga gtgccggcca gaagatgatc ttttcggcca cctatccgcc cctccccacc
     7141 ctgacccagg tgaccccgac agccttccct gcgactccgg taccgaccac ggccattctg
     7201 accctgccca cgacccccct gctgccggga tcgctctacg tgacgtcgtt cccgtcgggg
     7261 gcgaccatct acctgaacgg gagggggtac gggatcaccc cccggctcat ccagaacctc
     7321 acgccgggat cctacgagct ccggctctcc cttgtcggct gggatgacta ccagatgccc
     7381 gtctcggtga gccccggcct gaccgaggag gaacacgcca tcctccggtc ctgagagagg
     7441 agccatgcga tggcggatag gaggaaggtt ctctttgtct gcggccggaa tgcggcccgg
     7501 tcccagatgg ccgagggttt tctcagggcc ctgtacgggg atctctacga ggccgcgagt
     7561 gcgggactcc ggccttccgt cgtcagctct atcgccatcc gggtgatggg agaggcaggg
     7621 atcgacatct cctcccaccg gtccaaatcc ctggacgagt tctccggtga gcacctggat
     7681 ctcgtggtaa ctctctgcga cgatgcctcc tgcgtccggg atgccctcct tcccgatgca
     7741 gggcggtatc tccaccgggt gttccccgac cccgcggcct tcgccgtgag cgaggaggag
     7801 gttctggcgg cccaccggga tgtccgggac aggatagggg aatggatccg ggaggagttc
     7861 ggccccggga gaaccgtgcg gggaagcggc cgaaccccgc tcccaccacc tcccgatctc
     7921 caaaagacgt aaaaggagag agggccaaaa ctcctgttga ccatcatggg agtccgatat
     7981 ctcttctgtg ccccgatcat cctcgtgatc ctcggcgaaa ccgggtgtac cgcatgaacg
     8041 agagcaccct ccttctttcg gtggcgctca tcctcctcct cgccgtcccc gcctcggctc
     8101 tcgacagcgg cccgcaggtg ggggtctcct cgtacaccct ggaccccccg gccctcatga
     8161 agggcgacac cggcatcgtc accattcacg tggtgaactc ggggacggat gcctcgtaca
     8221 tcaactccgc acgcctctcg ggcggcgagg tcacggtgat ccagtccccc gatcagaccc
     8281 agggagggat cgcaccgggg agcggtatga cgtacgtgta caaggtacag gccgtggggc
     8341 cggacggcgt gtactacctc aagtttatcc tcgatttccg ggagggggga cagtattcct
     8401 tcaatatccc ggtcgagatc gaatcctcga ccctgaagct ctccatcctg gacatgccgg
     8461 acaccaccat ccagggagtg aggggtgagt accggatcat ggtcggtaac ccgaggccgg
     8521 atgccgtgaa cgcggtgacc atcaccccgg agggggacgg cttcctcgtg accccctcgg
     8581 gggtcttcct cgggaccctg ggggccgacg cctcatccgt cgtcaccttc aacgtgacac
     8641 ccctccgcca gaccttcatc cggttcaggg cgaactacaa gaacgggatc aacccccaga
     8701 ccaccaccct gaccctgccc ctctctactg tcgagaacag gctcgcggca gagcccgtcc
     8761 tcacgaacat ccacgtgacc gcggagcagg gggcgatcca cgtctcgggg gatgtgacga
     8821 acgtcgggct cgagacggcc cgctcggtga ccatcaccac ccagggaacg gtgacccccg
     8881 ccgatcccta caaggtcttc gtcgtcgggt ccctggaacc ggatgacatc tcctccttcg
     8941 aggtgacctt ccggtccaag gcggacatca ccgaggtccc gctcctcgtc acgtacaagg
     9001 atccggccgg gaacgtcatc accgcgacct cgaacgtgga gatcagcagc caggccctct
     9061 ccgaccaggc cagcgagccg tcccggttca cgaacctgct catctggatg tttgtcgtcc
     9121 tcgtcgcggc cgttttcatc tacttctgga gacggggttg aaggaggccg tgaccggggc
     9181 ccgccccggt ccctttgcca ccgctgcgag ggacatccgc gagcactggg tccggtccct
     9241 cttcgccatc gtggggatca ttatcggggt cgccgccatc tcgaccctgg gaatgctcgg
     9301 gaacgggctc gccctctccg tttccgattc catgaggtac gcgggcgaca ccatcgtcat
     9361 caccccccac gtgagcgcct cgccggggag cgccggggga gaaggatccg gcctgatgat
     9421 cacggagcac caggtcgggg agattcgccg ggccgcggca tcgaaccccg tcattcccat
     9481 ccgcaccggc gccgagcgca tcacggtggg atccggggct gaatcggtgg ccgtctatgc
     9541 catggccccc gcggatgtcc ccgttctcct cgagaaggcg agcggcaatt acctcaggta
     9601 cggctccggg gtcatggcag gaaagggact tgccgacgcg accgggctct cggtgggaga
     9661 taccatcggt atcggggccg gggaagaacg ggtgagggtg acggggatcc tcaaggagcg
     9721 gggaatcggg tttgacctca gcccggatga tgccctgatc gtccccgaca cctggttcgc
     9781 tgcccggtac ggggtccatg attacgacct ggtcatcatc agggtgaaga acccggatga
     9841 cattgacgcg gtgaaggcgg cggtcgatga acagctcaac ctgagggacc cggtggtgaa
     9901 catcctcgac acccggaggg tcctcaccac catcgtcgat agcttcaacc gggtctcggc
     9961 cgccatcatg acagcggggg gcatctccct cgccgtggcg ggagtctccc tcctctccgt
    10021 gatgctcatg tcggtgaccg agcgggcggg ggagatcggg atgatccgct ccctcggggc
    10081 gacgaggagc gaggtgatgc ggatcttcct ctccgaggcc ctgatcctgg ggctcgcggg
    10141 aagcgccgcc ggggggatcc tctccctcgc gggaggatac ctggcctcgg gcggaatgct
    10201 ccagaccacg aagttcctcc tcgtcccggc caccatcgtc cccatcctca ccgggatggg
    10261 ctttggcatc agtgtctccc ttctcgcggg gatttacccg gcctggaagg ccgcgatgat
    10321 gagccccgcc ggggcgcccc gggccgggta aagggtatgg gcacaccctc ctctctgaaa
    10381 aatccaccca gggagtgccc atgcccatcc ggtaccaact gcacccgtgg ccttcgggag
    10441 gcggggatcg ggtgcgttct ccttctcctc gtcctctctc tgatcccctt ctgcactgct
    10501 cagaccgggg gaggtggagg cctgccgctg gcaacagaat tccaaaaatt ccggagggtg
    10561 gtgatccttg gatatgccga agacgcgatg gagcctttca tctcgcagga cgggcagatc
    10621 ctctttttta acagcctcaa tgacgggaag gacacgagcc tctaccttgc ccgccggtcc
    10681 ggcgatgcca ccttccagct cgtcgggaag gtgggtggtg caaatggccc gccaccccac
    10741 ctggacgccg tggcgagtat ggatgcggcg aaccggttct acttcgtatc caccagggac
    10801 tatccagcgg cagacgagaa cctctttgtg ggcagcttcc gcgacggggc catcaccggc
    10861 gtcagacctg tgcaggggga cttctacctc cgccagccgg gctggatcgt catggacgcg
    10921 gagattcatc ggggaggaga ggttctcctg tatgtggatg cccatttcac cgggggggcg
    10981 atgccagacg ccgcattcat cggcgccgcc cgccaagtga acggcacctt tgccaccgac
    11041 ccgaacaacg gcatcctgtt ccgcgaggtg aacaccggtg acctgaacta tgccccgtcc
    11101 ctctcagccg atggactgga gctcttcttc acccgggcgg cggggacgac aacccggctc
    11161 ctcgtttcca ggagggattc tctcaccgag ccctttgggg ctccccagga gctggctata
    11221 cggggggagg cggtggaggc gccctgcatc agccttgacg gaaggttcct ctatttccat
    11281 atgagggaag gcgggaaatt tgccatcttc gtagcgtcta ggaactgaat ccgggtcctc
    11341 tcttcgaagg aattcctccc ggaaaagtac gggaaccgga atccggaatc aggaagagag
    11401 aattattcgt cgcttgtacc aggtcaggca ggagtgtgcg ccatttttaa aatgctcttt
    11461 tttatacatt ttatgacaaa ataataccac aatgtatata tcaacccgtg aacaacctga
    11521 ggggtatgca gtccaagttc cagcttgacg aggaccagat cccgaagcgg tggtacaaca
    11581 tccaggcaga cctgccgtcg cccatggatc cctactacag ctcgcagacg atgaagctgg
    11641 ccacgcccga ggagctccac cagatcttcc ccatggccct catcgggcag gaggtctcgc
    11701 aggaccggta catcgacatc ccggaggagg tccgggagat ctaccggctc taccggccca
    11761 gccccctcta ccgggcccac cggctcgagg cgcacctgaa gaccccggcg aagatctact
    11821 tcaagtacga gggggtctcc ccgccgggaa gccacaagcc caacacggcg attccccagg
    11881 cgtactacaa catgaaggag gggatccagc ggatctcgac ggagaccggg gccgggcagt
    11941 ggggatctgc cctcgccttc gcgacggccc tctttggcat gaagtgcacc gtgtacatgg
    12001 tccgctccag cttctacgag aagccctacc ggaagtccat gatgcaggca tggggtgcgg
    12061 agtgcatccc gagcccgtcc gaccggaccc agtcggggaa ggcgatgctt gcgaaggacc
    12121 cgaagacgcc gggctccctc ggcatcgcca tttccgaggc ggtggaggac gcggctaccc
    12181 acaaggacac caactactcc ctgggctcgg tcctgaacca tgtctgcatg caccagacca
    12241 tcatcggtca ggaagccatg gagcagctca agatggccgg ggacgagccg gacatgatca
    12301 tcggctgcgt cggaggcggc tccaacatgg cggggatctc cttcccgttc ctcggcgaga
    12361 agctcagggg gaaaaggaag gacctcgagg ccatcgcggt ggagccggcc gcgtgcccca
    12421 ccctcacgaa gggggtctat gcctacgact acggggacac cgtggggatc gggcctatcg
    12481 ccaagatgta caccctgggc cacgacttcg tcccgcccgc catccatgcc ggggggctcc
    12541 gctaccacgg cgactcgccc ctcatctccc ggctcgtcca tgacaaggtg atgagggcgg
    12601 tggccgtgca ccagaccgag gtcttccagg ccgcggtcac cttcgcccgg accgagggga
    12661 tcatcatcgc cccggagacg gcccatgcgg taaagacggt catcgacgag gccctcaggt
    12721 gcagggagaa cgccgaggag aaggtcatcc tcttcaacct ctccggccac ggcaacctgg
    12781 acctgggagc gtacgacgag tacttcgagg ggaagctctc ggacttcgag taccccgaca
    12841 gcaagatcaa ggagtccctg gcccacctgc ccaaagtcgg gtaggtctct ccagaggggc
    12901 ccctctcccc cctggatgct cccggctccc tgggggaccc gggatcccct cccccccttc
    12961 gcctcccctc cctttttcgg acaaagggtt aattcccttc cctgataaga ctctttcaat
    13021 gctcctccgg tacgcgggct gcctcctcgg ggctgccatc ggggacgcca tgggcatgcc
    13081 cggggagggg accccggcgg acctctggcg ggcggagtgc tcctaccggc gtgcctaccg
    13141 gggacacccg aacgaggccc tggcaccggg ccagttcacc gatgacaccc agctcctcct
    13201 cctctcggcc gggctcctcg ccgaccgggc cttcaccccg gagaagtacg ccgagcggct
    13261 cgccgaacgg taccggtcgg gtgatctccg gttccccgac ggctcggtcg tcgcggcctg
    13321 cctccacctg atggagggga cggcttcgga caggagcggg gtgaactcca ccaccgcggg
    13381 gtgcatcccg gcggggatcc cctttgccct cgcataccgg gacccggtgg agatccggga
    13441 aacagtggca cgggtgacga gcatcaccca cacgaacccg gccgccatcg cagggtgcac
    13501 cggcattgcg ctcctcatcc accacaccct ggcgggatcc ccggatgacc tcgacatcgc
    13561 ctggggggag gtgagccggg aggatgtcct gctcggcgag aagatccggc acgccctgta
    13621 cctcgagagg gaggggatcg gcctcgagca cgccatccgg ctcatcggga acgacctctc
    13681 ggtctaccag accctccccc tcgccttctt cctcatggcg aggtgctcga acgcggccga
    13741 gctcctctcc ctggtcggcc atgtcggggg gaacacctcg accatcggcc tcatctgcgg
    13801 cgcgtacctg ggggcgaagt acgggatgaa cgtcctcccg cggcagctca tcgaggggct
    13861 ggaggaccgt gcccggatcg agaccctcgc cgcccgcctc ctcgaggtgt cgtcggacat
    13921 cccccggata ccctgaggcc cgggaataac ccttaaattc cccccgccga atatcttttc
    13981 ccatgaaatt cacgacttcg tcgctcaagg tcgggttccg atgaaccgct cccaaatcgc
    14041 gacttcgtcg ctcaaggtcg ggttccgatg aaccactccc aaatcgcgat tatcggcgcc
    14101 tcgggctata ccgggggcga cctgatccgg ctcctcctct cccactcctc cgccgaggtg
    14161 accatcgcca cctcccggaa gctcgtcggg aagcgggtcg actcggtcca ccccaacctg
    14221 aagggcctca ccgacctcgt cttcgagaac cctcccgtgg accggatgga tgccgacgtc
    14281 gccctcctcg cggtacccca caccgccgcg atggggtacg tccgggggct cctcgaccgg
    14341 gggatgaagg tcgtggacct ctcggccgac taccggctcc cgaaggaggt gtacgaggag
    14401 acctacgggg tcccccacac cgattacttc cccgccccct acggcctcac cgagctccac
    14461 cgggatgagg tgaagggggt ttccttcgtc tcgaaccccg ggtgcttccc gaccggggcg
    14521 accctcgccg ccgcacccct cgcgaagatg gcgaaggccg tggtcttcga ctcaaagacc
    14581 ggggtctccg gcgcggggaa ctccccctcg gcggtcaccc actacccgac ggtcgcggac
    14641 aacatcaacc cctacaagtg gacccggcac cggcacctcg ccgagatgaa gcaggagatg
    14701 gcccggctcg ggacgaaggg aggggtgtac ttcacccccc acctcgtgcc cgtcaaccgg
    14761 gggatcctga ccaccgccca catcctcctc tccgagccga tggagcagaa ggaggtggag
    14821 gcgatgtacc ggaagttcta tgagaaggag ttcttcgtac ggctccagca gcccatcctc
    14881 gcggccgtgc gggggacgaa cttctgcgac atcgccgtgg agagcgaggg ggagcgggtg
    14941 gtcgcggtct cggccatcga caacctggtg aagggtgcga gcggccaggc gatccagaac
    15001 atgaacctca tgtgcgggtt ctcagagcag gacggcctca ggggggccgg ggtcctgccc
    15061 taggagggat catgaaggtc agggacgtga tgaccccgga ccccgtcgtg gtatcggccg
    15121 atgcgacgct ccgggaggcg gcggggctcc tccggaagaa caggatcggg gggctcccgg
    15181 tcatggacgg cgagaggctc gcggggatga tcaccgaatc ggacatcctg cggctcctga
    15241 agacacccgg ggtatccggg gatctctggc tcccctcccc cctcgagatc atcgaggtcc
    15301 cgatccggga ggccatcaac tgggcgcaca cgcagaaggc cctttcccat atcggtgacc
    15361 agccggtgcg ctcggtcatg tcctcccctg ccatcaccat cggtgagagg gaggacatgg
    15421 acgccgcggc cgccctcatg gtgaagaagc ggatcggccg gctcccggtg gtcaggggaa
    15481 ggaagctcgt cgggatcctc gcaagggccg acatcgtgag gggcctcggg gagtcctacg
    15541 ggggcacgaa gagcggggag gaggccccgt gaagagcatc tgcggcgttg agggcgtcac
    15601 cgccgccggg atgaaggagg gccggtacgg tctcgctctc gtggcggcga gcgggacggc
    15661 cgcggcggtc ttcacgagca acctggtgaa ggcggcaccc ctgatcctca tgcaggagcg
    15721 gatcgcccgc ggggagctcg acgggatcat cgtgaacagc ggctgcgcga acgccttcac
    15781 cggcgagagg ggccttcggg acgcggcgct cttggccgag atcggggcgg ggagcttcag
    15841 aaccgacccg gagcgggtcg gggtcgcatc gacgggaatc atcgggcgct acctggatct
    15901 ggacctgatc cggaggcagg cgggggaggt ctcgccccga atgaggaggg accccgctgc
    15961 cgaggtggag gccgcccggg ccatcatgac cacggacctc gtcgagaagc acgccctcat
    16021 ccccggggac ggcttctcgg tcggggggat ctgcaagggg agcgggatga tcgccccgaa
    16081 catgggcacg atgatcgccc tcctctacac cgacgcggag gtccctgcgg ggtcgctccg
    16141 gggggccctc cggacggcca cccgccgttc cttcaaccag gtggtggtcg acggggacac
    16201 ctccacgaac gacatcgcct tcctcaccgc cacgggggag gccgggaagg tcaggccggc
    16261 cgcactcggg aaggccctcg aggagtgctg ccgctccctg gccatccaga tcgcccggga
    16321 cggtgagggg gccacgaagc tcatcgagat cgtggtcagg ggagcccgga ccgagaaggg
    16381 ggcctccctg gtggcgagga ccgtggtcgg gtccccgctc ctcaagaccg cggtgtacgg
    16441 cgaggatccc aactggggcc gggtcctcgc tgccgcgggc cgggcggggg tggagttcga
    16501 cccggaccgg gtctccctca ccatcggcga aggggagggg cggacccccc tcgtccaagg
    16561 agggaggatc gtcgtcgacc tcgcgagggc gaagcaggcg atgaagggaa aggaggtcag
    16621 gttcgagctc gacctcggag agggcaaccg ggaggccacg gcctggggct gtgacctcac
    16681 cgagaagtac gtggagatca acgggaggta cacgacgtga aacgggaaga ggtgctcatg
    16741 gaggcgctcc cctacatcca gcgcttccac gggaagacca tcgtgatcaa gctcggcggg
    16801 catgccatgg tggaccagga gatcctcgag atggccatcc gggacgcggt cctcctccag
    16861 tacgtgggca tgaaggtggt catcgtccac ggcgggggac ccgagattac cgagaagatg
    16921 caggccatgg gcaaggagcc gaagtttgtc ggagggctcc gggtcaccga tgccgagacc
    16981 ctggagatcg cccagatggt cctcgtgggg aagatcaatg acgggatcgt ctccctcatc
    17041 gcgaagggcg gggcgagggg ggtggggctt tccgggaacg acgggaacct catgatcgcc
    17101 cggaagatgg atccccagaa ggtgaaagag gggaaccggg tgaagaaggt ggacctcggg
    17161 ttcgtgggcg agatcgagga ggtggacccc accctcctca acaccctcct cgacaggggc
    17221 tacatcccgg tcatcgcccc catcgccatc gaccgggagg ggacgagcct gaacatcaac
    17281 gccgacacgg cggccggtga gatcgcggtc gccctcgggg ccttcaagct ggtcaatctc
    17341 accgacgtag acggggtgat ggattcggga aggacccagg tcttccgccg gctcaccacg
    17401 caggagctcg agaagctcat cgcggacggg accgtgagcg gcggaatgat cccgaagatc
    17461 acctcctcac tccagtccgt gaaacggggg gtcgggcagg cccacatcat caacgggaac
    17521 caggggcaca acctcctcct cgagctcttc accgatgccg gcgtcgggac gatgatctcc
    17581 cggtaagggg atcccttccc catcccgttt cgcccgtggg atactacctt ttataaagaa
    17641 agcaaatact cacgtggggt gatacattct tggatatctt ttcgatcatc atcctcgtca
    17701 tcgtcgtcat cgtcatcctt gcccttatct tctggttcgt gggcatctac aaccagttct
    17761 acaagtacag gaacgccgcc gatgcgaccc tcgggcagat ccgggtggcc atgaaaaaaa
    17821 ggctcgacat gatcgagcag ctcctcgggg ccgtgaagag ctatgccaag ttcgagaagg
    17881 agaccctcac gaagatcacc gagatgcggg cgagcgtgct caaggggacc ccctccgagc
    17941 tgaacaccgt cgagagggag tcccgcgccc tcctggggac cctcatcgcg accgcagagg
    18001 cctacccgga cctcaagacc tcctcaacgg tcacctctct catggactcg gtgaaggggg
    18061 tcgaggagga gatcgcgagg cagcggtaca cctacaacaa catcgtccag cagttcaaca
    18121 ccatgcttga tgttctcccg tcgagcttta tcggccggct catcgggctc ctgaagctcg
    18181 actacctcca gttcgaggag gccatcagca cgccgccgaa gatcgagttc taggcgggcg
    18241 tccgagacag gcataccatg agcgagaacc ggcagatcgc caccctgatg gccatcaccc
    18301 ttgtcatcgg ggtgatcgcc ctcgcccttt cccttgccct ccccggcgcc ctcgagggga
    18361 acctggtcat cgaccggtac accgcgagtg tctctgcaga cgggacgctc tccgagcact
    18421 acgactatgt cgtgaaggac ccgggggtgt accggttcct cttccgcacc tgggaggtgc
    18481 ccctcgcggg gtcggccctg ccccagccct ccgtcgaggt catcgggatt aaggtcccgg
    18541 cggggaccgc cgggtattac cgtgactacc gaggacaggt caccatctac gggattacca
    18601 ccgcggagga gaggtccacc atccagtccc tcgtccagga caacgagatc ggtttctata
    18661 ccccctccta tttcccggcg ggaacctaca gcgtggactt tacctaccgg atccatcccc
    18721 ccatcgagta cgacccccgg tggagccacc tgaacctgcg gttcgccgac cagcatgtcc
    18781 cctaccacgc catccaggta tccataccct cggcaggggt ggaatcggtg tatccctacc
    18841 cgcccttcct cgcggtgatg cagagcgggg ggaccacaac catttccggg agcgcggcag
    18901 cgaacgaggt ggtgggggtc gagatcgtcg catccaggga ctacacggtg aactggcagg
    18961 gtttcccgga cgcggtctct gatgtgcagg gccttgcgga gtcggccttc ttctggtaca
    19021 gcctccccta ctatgccggg gccctcctcc tcgccatcgc ggggatcctg gtcctcatca
    19081 tgccggccct cctctacatc gtgtacaacc ggtacggccg ggagaaggag ttcaccgtcc
    19141 ccgaatacct ctccttcgtc cctgaccgga ccctcccgcc ctgggtggtg aacctggtct
    19201 tcaaggacga ggccctcacc tttgacgaga acggcttcta tgccacggtc ctcgatctcg
    19261 gtcggaagaa gttcctcgag atcaagccca agggggagtc ggcagaggcg ggggtcacca
    19321 tccgggtcct ccaggggacc tcccccgatc cctacgaaca gagggtcctc aacttcctcc
    19381 ggaacctggc ggacgggggc gtggtggaca cggacgccct gaaggggctc accgagtctg
    19441 cccgggcgga ccggtcctcc gagctccggg ccatgaagat ccaggccacc ctccggggga
    19501 tcaccaccga ggtggatccc accgtggcga accggttcgt catcgacggg aggccccacc
    19561 tcgtcccgct cctcctcggc tcggcgatcc ttgccgggct ctccatcctg atcctcgtga
    19621 tcggctccca ggcggtgacc ctcctccttc ccgccgcggt tctcggctcc gtcgcggtca
    19681 tccaggtggc catcgcctgg gccttcccct ccaccctctt cggccagtgg aagggggact
    19741 tctaccagga gaagctccag tgggacgcct tccggaagtt cctctccgac ctcgcccgga
    19801 tcaaggaata cgcccctcag gatatcgcca tgtggggcga gtggctggtc tacgggacgg
    19861 ctctcggcgt cggggataac gtgacaaagg cgatgaagga gctgaacatc cgggtcccgg
    19921 agatgtacgt cgtgccggct ctctacctcg gatttgctcc gatctggggg ttcgcgcccc
    19981 cctcccgggg aggtatggga ggaggcgggt tccacggcgg tggcggattc ggcggcggcg
    20041 gaggcttcgg aggaggcggg gccgggggga ggtgaacccg acaccgtcgc ccattttttt
    20101 aatttcaatt ttctttatcg ccgcattccg gaatactcct gcgttagatc cgccgcagcg
    20161 gcggcctccc agcaggtccc tgcgataagg ggctcttcac gggaggttcc cgtcgcccat
    20221 gcactcctgc tgccgctcct cgctcatctc gacgaggatg ccgaagatct tctggaccga
    20281 gacaggatcg accttggcct cgacggcccg gtcaaaggcc cggtcgagga catgctccct
    20341 ctgc
//
LOCUS       DATGDI010000194         3935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_339736, whole genome shotgun sequence.
ACCESSION   DATGDI010000194 DATGDI010000000
VERSION     DATGDI010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339736"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VKO45_07160"
     CDS             <1..923
                     /locus_tag="VKO45_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HMA05690.1"
                     /translation="PRTGKPLMERTILIANTSNMPVAAREASIYTGITIAEYYRDMGY
                     DVALMADSTSRWGEALREVSGRLEEMPGEEGYPAYLATRLAAFYERAGRVVCLGSGGR
                     NGSITVVGAVSPPGGDFSEPITQNTLRIAGTFWALDTSLAYRRHFPSVNWIKSYSLYL
                     ESIRDWYGESGFGDWKDLRDRTMYLLQKEVELQEIVQLVGPDALPESEKAILEVTRMI
                     REDFLQQSAYDKIDSFCPMEKQYWMLRVILAFYDRVSEAMAKGIALSKILKLPVKAEI
                     GRMKELSEIERIKGLIPGIEKAVATLEVER"
     gene            920..2317
                     /locus_tag="VKO45_07165"
     CDS             920..2317
                     /locus_tag="VKO45_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HMA05691.1"
                     /translation="MIPKSLAVKESRTVSYVSGPLIFVRFVKGVSFGEIAKIILPSGE
                     VRTGQVLDVSRDLAVVQVFEGTSGIDNNLTRVRFTGEAARIDVSIDMLGRIFDGRGKP
                     RDGGPDIIPEASLDINGMPINPYARDKPADFIQTGMSAIDALNTLVRGQKLPIFSGAG
                     LPANKLAAQIARQARVLGEEEVFAVVFVAMGITYKDASFFMRDFERTGALDRVVFFMN
                     LADDPTIERIATPRCALTVAEYLAFTHNMQVLVILTDMINYCEALREISTAREEVPGR
                     RGYPGYMYTDLASIYERAGRIKGKKGSITQIPILTMPDDDITHPVPDLTGYITEGQIV
                     LSRELFRRGADPPIDVLPCLSRLMNLGIGPGKTREDHRNVADQLYALYAYGRDLRRLV
                     AIVGEEALTEMDKKYLKYADECEKKFITQEDENRSIEKTFSIAWDLFTMFPHEELKKI
                     KMEYIQKYHPEHPKG"
     gene            2322..3011
                     /locus_tag="VKO45_07170"
     CDS             2322..3011
                     /locus_tag="VKO45_07170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HMA05692.1"
                     /translation="MEQANPTRMELIRKNAQIKLAEQGRDLLREKMDALIQEFFRIMQ
                     AVSRSRVELEAMADVAQQSLLIALATDDPVTVKSASFATKRGLTLDITGKNIMGVPVP
                     VIEKKRISKSVFERGYSIIGVSGRIDEAAEKFEGELDLVIGLAETETALKRLGGEIQM
                     TRRRVNALEQVLIPQLKSQAKYIENTIDEREREDLFRLKKVKKIIERKKKASEAVAAA
                     ASVTPSSIISG"
     gene            complement(2983..3312)
                     /locus_tag="VKO45_07175"
     CDS             complement(2983..3312)
                     /locus_tag="VKO45_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05693.1"
                     /translation="MPEVTSDERGRRVFQIHKEKAAEDAIGKIRKSLSIDWTVFNSTD
                     VEALRYILEETWVSLDRDSWEKCAFSRLKRSQVDSIIRIGKQVKRRELREDRGIEEVS
                     RILSTMA"
     gene            complement(3397..3726)
                     /locus_tag="VKO45_07180"
     CDS             complement(3397..3726)
                     /locus_tag="VKO45_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05694.1"
                     /translation="MPDVSDQERGRRVFQIQKEKHAEEALEKVRKKLGDGWKRFSLQE
                     IRTLRFILGEAWVFTDRKDWEMYSFARLSLDDLDAILAIGKAVEKKLKTEHDASEEVG
                     AILQRVP"
BASE COUNT          812 a         1211 c         1178 g          734 t
ORIGIN      
        1 acccgaggac ggggaagccc ctgatggagc ggaccatcct catcgcgaac acgagcaaca
       61 tgccggtcgc tgcccgggag gcctcgatct ataccggcat caccattgcc gagtactacc
      121 gggacatggg ctatgatgtg gccctgatgg cggactcgac ctcgcgatgg ggagaggcgc
      181 tgcgggaggt atcagggcgc ctggaggaga tgccagggga ggaggggtat ccggcgtatc
      241 ttgccacgcg gcttgcggcc ttctacgaac gggcggggag ggtggtatgc ctgggcagcg
      301 gggggaggaa cggctccatc acggtcgtcg gcgcagtctc cccccccggg ggggatttct
      361 ccgagcccat cacccagaac accctccgga tcgcaggcac cttctgggcc ctggatacga
      421 gcctggccta ccgccgccac tttccttcgg tcaactggat caagagctac tccctgtacc
      481 ttgagagtat cagggactgg tacggtgaga gcggctttgg ggactggaag gatcttcggg
      541 accgcacgat gtacctgctg cagaaggagg tcgaactcca ggagatcgtc cagctggtgg
      601 gtcccgacgc gctccccgag agcgagaagg cgatcctcga ggtcacccgg atgatccgcg
      661 aggatttcct gcagcagagt gcctacgaca agatcgactc cttctgcccc atggagaagc
      721 agtactggat gctccgggtg atccttgcct tctatgatcg cgtttccgag gcgatggcca
      781 aggggatagc gctctccaag atcctgaaac ttccggtgaa ggccgagatc gggagaatga
      841 aggagctctc ggagatcgag cggatcaagg ggctgattcc cggaatcgag aaggcggtcg
      901 ccaccctcga ggtggagcga tgatcccgaa gagccttgcc gtcaaagaga gcaggaccgt
      961 gagttacgtc tccgggcccc tcatctttgt gcggttcgtc aagggggtct ccttcggcga
     1021 gatcgcaaag atcatcctcc cctccggcga ggtgaggacc gggcaggtcc tcgatgtctc
     1081 ccgcgacctc gccgtggtgc aggtctttga agggacgagc gggatcgaca acaacctcac
     1141 ccgggtacgg tttaccgggg aagctgcccg gatcgatgtc tccatcgata tgcttggcag
     1201 gatcttcgat ggcaggggga agcccaggga cgggggaccg gatatcattc cggaggcctc
     1261 cctggacatc aacgggatgc ccatcaatcc gtacgcccgg gacaagcccg cggacttcat
     1321 ccagaccggc atgtccgcca ttgacgccct gaacacgctg gtgcggggcc agaagctccc
     1381 catcttctcg ggagccggcc ttcccgcgaa caagctggcg gcccagatcg cacggcaggc
     1441 ccgggtgctc ggcgaggagg aggtctttgc cgtggtcttc gtcgcgatgg ggatcaccta
     1501 caaggacgca tccttcttca tgagggactt cgagcgcacg ggggcactcg atcgcgtcgt
     1561 cttcttcatg aaccttgccg atgacccgac catcgagcgg atcgccacgc cccggtgtgc
     1621 tctcactgtc gcggagtacc ttgccttcac ccacaacatg caggtgctcg tcatcctcac
     1681 cgacatgatc aactactgcg aggccctccg ggagatctcc actgcccggg aagaggtgcc
     1741 gggccgcagg ggctaccccg gctacatgta caccgacctt gcctccatct atgaaagggc
     1801 aggaaggatc aagggaaaga aaggctccat cacccagatc cccatcctga ccatgcccga
     1861 cgacgatatc acccacccgg tgccggacct caccggctac atcaccgaag gccagatcgt
     1921 gctctcgcgg gagctcttca ggcgaggggc cgacccgccc atcgacgtgc tcccctgcct
     1981 ctcccggctg atgaacctgg ggatcgggcc ggggaaaacc cgggaggacc accgcaacgt
     2041 cgccgaccag ctctatgcat tgtatgccta tggaagggac ctccgcaggc tcgtggccat
     2101 cgtgggggag gaggccctca ccgagatgga caagaagtac ctcaagtatg ctgacgagtg
     2161 cgagaagaag ttcatcaccc aggaggacga gaaccgttcc atcgagaaga ccttctccat
     2221 cgcatgggac ctcttcacca tgttccccca tgaggagctg aagaagatca agatggagta
     2281 tatccagaaa taccaccccg aacacccgaa gggatgatgt catggagcag gcaaacccga
     2341 cgcggatgga gctgatccgg aagaacgccc agataaagct cgccgagcag gggcgggacc
     2401 tcctccgcga gaagatggac gcgctgatcc aggagttctt ccggatcatg caggcggtct
     2461 cccgttcacg ggttgagctc gaggcgatgg cggacgtggc gcagcaatct ctcctcatcg
     2521 cgctcgcgac agacgatccg gtcacggtaa aatcggcgtc atttgcgaca aagaggggac
     2581 tcacgctcga tatcaccggg aagaacatca tgggtgtccc cgtcccggtc atcgagaaga
     2641 agcggatctc aaagtccgtc tttgaacggg gctacagcat catcggcgta tcgggaagga
     2701 tagacgaagc ggcagagaag ttcgaggggg agcttgatct cgttatcggt cttgcggaga
     2761 cggagacggc gttaaaaagg ctcggcgggg agatacagat gacccgccgg agggtgaatg
     2821 cgctggagca ggttctcatc ccccagctga agagccaggc aaagtacatc gagaacacca
     2881 tcgatgagcg ggagcgggag gatctcttcc ggctcaaaaa ggtgaagaag atcatcgagc
     2941 gcaagaagaa ggcgtccgag gccgttgccg ccgcagcatc cgtcacgcca tcgtcgataa
     3001 tatccggctg acctcctcga taccgcgatc ctccctcagc tctcttctct tcacctgctt
     3061 cccgatccgg atgatcgagt cgacctgtga tcttttcaac cgtgaaaagg cgcacttctc
     3121 ccagctgtcc cggtccagcg agacccaggt ctcctcgagg atgtacctga gggcctcgac
     3181 atcggttgaa ttaaagaccg tccagtcgat gctcagggac ttgcgaatct tcccgatggc
     3241 atcctccgcc gccttctcct tgtggatctg gaataccctc cttccccgtt cgtcgcttgt
     3301 cacttctggc attacccacc ctcaccctct ctctcccatg atgtttcaaa aggtttccgg
     3361 taccgtgggg aagtcctgaa aagggacagg attccctcag gggacccgct ggaggattgc
     3421 ccccacctct tccgatgcat catgctcggt cttcagcttt ttttcaactg ccttcccgat
     3481 cgcgaggatc gcatcgaggt cgtcgaggga gagccgtgcg aaggagtaca tctcccagtc
     3541 cttccggtcg gtgaagaccc acgcctcccc caggatgaac ctgagcgtcc tgatctcctg
     3601 gagggagaac ctcttccagc cgtcgcccag cttcttccgg accttctcca gggcctcctc
     3661 ggcgtgcttc tccttctgga tctggaagac ccttcttccc cgttcctggt cactgacatc
     3721 cggcataccg tcccagcctc acgctaccat aatcgaggga gaacaatata ggtttcggcc
     3781 catcgggctt gcgaagcagg tgaaaacagg gccatgatga ggcatcggtt ccctccggaa
     3841 acctccacgg gggtgtcggt tccctccgga aacctccacg ggggtgtcgg ttccctccgg
     3901 aaacctccac gcactaaatg acaacacata tattg
//
LOCUS       DATGDI010000195         4794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_253220, whole genome shotgun sequence.
ACCESSION   DATGDI010000195 DATGDI010000000
VERSION     DATGDI010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253220"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..752
                     /locus_tag="VKO45_07185"
     CDS             129..752
                     /locus_tag="VKO45_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05695.1"
                     /translation="MGSRVKIAALLLLLLVFFSLQAGCISLGPPPQGGQQGQPSPKST
                     QTVRPGETSIQDITSRNAPKVSLEDAIALLPAAQQEGLIDMTGRVITKVWGYGVDSAG
                     LGRTWVLGMAGNGNTTLLSYTEGGWSVIDLPTTLPREELKMGELVSPQKLFNRNLNTI
                     IAEMNRLRVGEVDLTLDAGTYQVIIHSATESTTFSFNARTGELIASP"
     gene            749..2077
                     /locus_tag="VKO45_07190"
     CDS             749..2077
                     /locus_tag="VKO45_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05696.1"
                     /translation="MRENSGQLPVDFLVGFTIFILSLIMVANFVPSLLVGLQRTTGID
                     YNAVAYRTGVVLVEDPGQPSRWDNDPLLSKDLGYMIVAPLNPPWDQQTRDQQTANPTR
                     FGLALTRDTPNILSIDKIERFFNTSFYRDADYRNKLLFSSYPYGYNVTLQSISPMDPG
                     QYPNGLVRMLGEPYPAGYGYIRRYVVIKQDSNATIDLALRPEFNAANEADVNLVTHNN
                     PLETDPHQEFTIRLDGSVLYNKSIDAPFTIDLQNEPIRVRIDNLQFVLNNSALNTGSP
                     LANSTDQWDDIGDALDPSIADAWCPTNQLADRCIAPTSATLVEVQFWNTYYGKMIPSL
                     FQVNLSVDDGWASHRYVDWTSRINAGVHADDFDPPVQVYHSIALNVSPLRPEAINSDN
                     TLDIVFKFRDGTDGYALPHTLLKGTYLYDYNRTNVTQPVLSTGMLEVGVW"
     gene            2071..2640
                     /locus_tag="VKO45_07195"
     CDS             2071..2640
                     /locus_tag="VKO45_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05697.1"
                     /translation="MVKDGAQLYTMEGVAAGLIMLLTAYLVLGSTSVYTPGDTHITDM
                     QLEQVGSDILRVMDTPRILTSSLRSNRTDLELLVTTTDSTSFYNSFIGDYLCRATGPT
                     PVGRDITVGCTDFEINASIWYMNNTGTGNLGSYLLLNFVQPEAYTGTGPGVRVTHWIS
                     TNSKPSGSVPADMRPEPQVVLMEVVMWKA"
     gene            2628..3068
                     /locus_tag="VKO45_07200"
     CDS             2628..3068
                     /locus_tag="VKO45_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05698.1"
                     /translation="MEGLKSDGQWIIMMGFLVSLGIFFLAIIISQSTLVGQTTAEGVL
                     EFPKNDLQDIRHVVFEVEKFNRTPSSPGYGNSTENLNQEIQLMSLYRKSAIVWYTISP
                     PPPATCTPCPCTETKIRSVEIHYNNGVTMYDETASVPYETSNKC"
     gene            3046..3507
                     /locus_tag="VKO45_07205"
     CDS             3046..3507
                     /locus_tag="VKO45_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05699.1"
                     /translation="MRHPTSARRRDPTDGITNLVEYISITGILVLLMVVVLFTVNSVF
                     MQGPADTLKYSAFTDIGNGMSVRIVDLYVIAPDHGTISSRFILPKDVAGQDYFVRANE
                     TGEDRTTQTIVVERGDIHSDINIAGIGATKGVTGNTTSSGWNLITYNSSGV"
     gene            3523..4332
                     /locus_tag="VKO45_07210"
     CDS             3523..4332
                     /locus_tag="VKO45_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05700.1"
                     /translation="MKGKGEAVSESIGYLIIFSLVLTGIGLITLYGYPLLMQQQSSAN
                     MRNMEQTMIVLQNDIKSLTYKLANYRETSLRVGGGTLSLYGPLYTPQTFKIETDSGFS
                     FNPPPLGELRYISTADNTVITLENGAVIEKPLATPGSFMLADPRWYLDNSASQRTLVI
                     TFMNLSSATTEGQLLSQTGVGTVRMRLSGSRMTSPDWGGSPTNVRITYSPANTATITP
                     DGYALAWKTYLTNAVNFPGVTWSSVMGNDVYQFANINKIVIKEYDIIVETL"
     gene            complement(4375..>4794)
                     /locus_tag="VKO45_07215"
     CDS             complement(4375..>4794)
                     /locus_tag="VKO45_07215"
                     /inference="COORDINATES: protein motif:HMM:NF021420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2121 domain-containing protein"
                     /protein_id="HMA05701.1"
                     /translation="VILSIRDVKVTARERAGVLVGEVSSVEGGMLKSRRLYASAGSYA
                     ILDFGEASPTLRRRGGAGNFVVLGNERTKAIAHRCIREGWKNGNLRDAAAIIRQSLEE
                     AARASASVSREFVFLETREKADLERLMEQDGFRDQRA"
BASE COUNT         1042 a         1544 c         1269 g          939 t
ORIGIN      
        1 agattttcat aaggtatgta tctgaatatc accataattc gtgatgcagg aaaagatccg
       61 acaaggatct tcgaatccga aaaacccaag atttatattg aacttagtga tgataaaggg
      121 tgtacggcat gggatcccgg gtgaaaattg cggccctcct tctcctcctc ctcgtcttct
      181 tctccctgca ggcaggctgc atctccctgg gtccgccacc gcagggcggc cagcagggcc
      241 agccgagccc gaagagcaca cagaccgtca ggcccgggga gacctctatc caggacatca
      301 cctcccggaa cgcccccaag gtctctctcg aagacgccat cgcgctcctc ccggcagccc
      361 agcaggaagg cctcatcgac atgaccggaa gggtgatcac gaaggtctgg ggatatggcg
      421 tcgattccgc aggcctcggg agaacctggg tcctcggcat ggcggggaac gggaacacca
      481 cgctcctctc ctacaccgag ggtggatgga gtgtgatcga tctccctacc acccttccca
      541 gggaggagct gaagatgggg gagctcgttt cgccccagaa actgtttaac cggaacctca
      601 acaccatcat cgccgagatg aaccggttga gggtagggga ggttgatctc accctggacg
      661 cgggtactta ccaggtcatc atccattcgg ccacggagag cacgaccttc tccttcaacg
      721 cacggaccgg ggagttgatc gcatccccat gagggagaat tcggggcagc tcccggtgga
      781 cttcctggtc ggcttcacca tctttatcct ctccctcatc atggtggcga acttcgtccc
      841 gagcctcctc gtgggcctcc agcggaccac gggcatcgat tacaatgcgg tcgcctaccg
      901 gaccggcgtc gttctggtgg aggacccggg ccagccctcg aggtgggaca atgatcccct
      961 tctctccaag gacctgggtt acatgatcgt ggcccccctg aaccctccct gggatcaaca
     1021 gacaagggac cagcaaacgg caaatcccac ccgcttcggc ctggccctga cgagggacac
     1081 cccgaacatc ctctccatcg ataagattga gaggttcttc aacaccagct tttatagaga
     1141 cgccgattac cggaacaagc ttctcttctc ttcttacccc tatgggtaca acgtcaccct
     1201 gcagagcata tcccccatgg atccgggcca gtacccgaac ggcctcgtac ggatgctcgg
     1261 ggaaccctac ccggccggtt acgggtacat tcgtcggtac gtggtcatca agcaggactc
     1321 caatgccacc atcgatctcg cccttcgccc ggagttcaat gcagcgaacg aggccgacgt
     1381 gaatcttgtc actcataaca atcccctcga aacagacccg catcaggagt ttaccatacg
     1441 cctcgacggt tctgtcctgt acaacaagag catcgatgcc cccttcacca tcgaccttca
     1501 gaacgaaccc atcagggtgc ggatcgacaa cctccaattc gtcctgaaca actcggccct
     1561 taacactggc agccctctcg ccaattccac ggatcagtgg gacgacatcg gcgatgccct
     1621 cgacccctcc attgccgatg cctggtgccc cacgaaccag ctggctgacc ggtgcatcgc
     1681 gcccacctcc gcgaccctcg tcgaggtcca gttctggaac acctattacg ggaagatgat
     1741 cccctccctg ttccaggtaa acctgagcgt tgacgacgga tgggcctccc atcgttacgt
     1801 cgactggaca tcccggatca atgcgggggt acatgccgac gatttcgatc cgccagtgca
     1861 ggtctaccat tccatcgcat tgaatgtaag ccctctccgg cccgaggcca tcaactccga
     1921 taacaccctg gacatcgtct tcaagttcag ggacggcact gatggttacg ccctccccca
     1981 caccctcctc aagggtacct acctgtacga ctacaaccgg acgaacgtga cccagccggt
     2041 cctctccacc ggcatgctcg aggtgggcgt atggtgaaag acggggccca gctctacacc
     2101 atggagggag tcgcggccgg tcttatcatg cttttgactg cgtacctcgt cctcgggtcc
     2161 acctccgtct ataccccggg tgatacccac atcaccgata tgcagctcga acaggtggga
     2221 agtgacatcc tcagggtgat ggacactccg cggattctca ccagttccct gaggtccaac
     2281 cggaccgacc tcgaacttct ggtgactacc acggattcca ccagtttcta caacagtttc
     2341 atcggtgatt acctctgcag ggcgacggga cctacgccgg tgggcaggga catcacggtt
     2401 ggatgcacgg acttcgagat caatgcgagc atctggtaca tgaacaacac gggaacggga
     2461 aacctgggga gttacctgct gctcaacttt gtccaacccg aggcgtacac gggaacggga
     2521 cccggggtca gagtgaccca ctggatcagt accaattcta aaccgtcggg gagtgtcccg
     2581 gcggacatga gacccgagcc tcaggtggtc ctcatggagg tggtcatgtg gaaggcctga
     2641 agagtgacgg gcagtggatc atcatgatgg ggttcctcgt ctccctcggg atcttcttcc
     2701 tcgccatcat catcagccag tccaccctgg tgggccagac cacggccgag ggcgtcctcg
     2761 agttcccgaa gaacgacctc caggatatca ggcatgtcgt cttcgaggtc gagaagttca
     2821 acaggacacc ctcatctccc ggatacggta actctacgga gaacctcaac caggagatcc
     2881 agctgatgtc cctgtaccgg aagagtgcca ttgtctggta taccatttca cccccgcccc
     2941 ctgcgacatg tacaccgtgt ccatgcacgg aaacgaagat cagatcggtg gaaatccatt
     3001 acaacaacgg agtgaccatg tacgacgaga cggcgagtgt gccttatgag acatccaaca
     3061 agtgctagga gacgggatcc aaccgacggg atcacgaacc tcgtggagta catatccatc
     3121 accgggatcc tcgtcctcct catggtggtc gtccttttca ccgtgaactc cgttttcatg
     3181 caggggccag cggacaccct gaagtattcc gccttcacgg atatcgggaa cgggatgagt
     3241 gtccgcatcg tggatctcta tgtcatcgca ccggaccacg gcaccatctc cagccggttc
     3301 atcctcccca aggatgtggc agggcaggat tacttcgtgc gggccaatga gacgggagag
     3361 gacaggacaa cccagaccat cgtcgtggaa aggggggata tccattctga catcaacatc
     3421 gccggcatcg gcgccacgaa gggtgtgacc gggaacacca cgagctcggg ctggaacctc
     3481 atcacctaca attcctcggg agtctgagag ggagggttac ttatgaaagg gaagggagaa
     3541 gcagtatcgg aaagcatcgg gtacctgatc atcttcagcc tcgtgctcac gggcatcggg
     3601 ctcatcacgc tctacgggta cccgctcctc atgcagcagc agagctcggc taacatgcgg
     3661 aacatggagc agacgatgat cgtcctccag aacgatatca aatccctgac gtacaaactc
     3721 gcgaattacc gggagacctc cctcagggta ggcggcggaa ccctgtccct gtacggcccc
     3781 ttatacacgc cccagacctt taagatcgaa acggactccg gcttctcctt caacccccca
     3841 cccctcggtg aactccgcta catctcaacg gcggataaca ccgtcatcac cctggagaac
     3901 ggggcggtca tcgagaagcc cctcgctacc cccggatcct tcatgctcgc cgatccccgg
     3961 tggtacttgg acaattcggc cagccagagg acccttgtca ttaccttcat gaacctgagc
     4021 tcggctacca ccgaaggcca gctcctctcc cagacaggcg tcgggaccgt gcggatgcgg
     4081 ctgtccgggt ccaggatgac gtccccggac tggggcggct cccccaccaa tgtaaggatc
     4141 acctattcgc cggccaacac cgcgaccatc acgcccgacg gctatgctct cgcctggaag
     4201 acctacctga cgaatgcggt taatttcccg ggggtgacct ggagctccgt tatgggaaac
     4261 gacgtgtacc agttcgcgaa catcaacaag atcgtcatca aggaatacga tatcatcgta
     4321 gaaaccctct gatatttctc gagaggcaat cctccttttc cgcgggttca gatctcaggc
     4381 ccgttgatcc cgaaacccgt cctgctccat caacctctcc agatcggctt tctccctcgt
     4441 ttccaggaag acgaattcac ggctgaccga ggcgcttgcc cgggctgcct cctccaggga
     4501 ttgcctgatg atggcggccg catcgcgaag gttcccgttc ttccagccct cccggatgca
     4561 ccggtgggcg attgccttcg tcctctcgtt cccgaggacg acgaagtttc cggcgcctcc
     4621 tcttctcctc agggtggggg aggcctcccc gaaatcgaga atggcgtacg atcccgcaga
     4681 ggcgtacaac ctccggctct tcagcatgcc accctcgacg gaggaaacct ctcccacaag
     4741 cacccccgcc ctttcgcggg ccgtgacctt gacatcccgg atggagagga tcac
//
LOCUS       DATGDI010000196         1958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_282461, whole genome shotgun sequence.
ACCESSION   DATGDI010000196 DATGDI010000000
VERSION     DATGDI010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282461"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1315)
                     /locus_tag="VKO45_07220"
     CDS             complement(2..1315)
                     /locus_tag="VKO45_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HMA05702.1"
                     /translation="MSDLWILMLIPLCIALEGIFSGGEIAFLASDPNIIRPREKDGIP
                     SARLALRLLDRPEWFLATTLTGTVLCVTTSTVLATSLLIGHFGPARGEWISVAIMVPI
                     VLIFGEILPKSICRQHAEKWAMTIAPFLWVASWVLYPLVFLISKIARGAVYVLAGERG
                     KLSLPYITKDGLKYLLLEEAADTDVKRSEKVMVERIFDFSEASAGRVMVPISNVAALE
                     DEANFGDAARLINETGFSRLPVYRGDIIDIVGVVNAFDILKQLPASAAQPVRTALRQL
                     LYVPASKPADDLLLEMQKRGEPMAVVVDEYGGAVGIVTIEDILEEIVGDIRDEYDKRE
                     RAVRKLGPGQYLVSAHIGIDRLREIVPVSIPEGPYETLAGYLLHQMGRIPRRMEQFRA
                     GNVKFVIEDADMKSIKQVQIILPAETSAAQKEEGQAEPQRPVVGL"
     gene            complement(1308..>1958)
                     /locus_tag="VKO45_07225"
     CDS             complement(1308..>1958)
                     /locus_tag="VKO45_07225"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HMA05703.1"
                     /translation="IMTPRVDMFCLPVSTSPQALRREIIEQGYSRIPLYGTGPDDIVG
                     ILYARDLLGLMAEGKAPASTEPLLRKPYFVPEERSADQVLRDFQKRNTHVALVVDEYG
                     GISGLVTMEDILEALFGDIYDERDTRERPFHRIDEQNLMVSGAMSIDDFNSIAGTSIP
                     SEDFGTVGGFVFHLFGRLPARGDGISYEGLSFVVRKVKGTRILNVRVMLGKGEGHE"
BASE COUNT          369 a          630 c          608 g          351 t
ORIGIN      
        1 gtcatagacc caccaccggc cgctgcggtt cggcctgccc ctcttctttc tgcgcggcgg
       61 aggtctcggc gggcaggatg atctgtacct gcttgatcga cttcatgtcg gcatcctcga
      121 tcacgaactt gacgttgccg gcgcggaact gctccatccg gcgcggaatc cgtcccatct
      181 gatggagaag atatcccgcc agggtttcat agggtccttc cggaatgctg acagggacga
      241 tctcccgcag ccgatcgatg ccgatgtggg cgctgaccag gtactggccc gggcccagct
      301 tgcggacggc gcgctcccgc ttgtcgtact cgtcgcggat gtcgcccacg atctcctcga
      361 ggatgtcctc gatcgtcacg atgcccacgg ccccgccgta ctcgtcgacg accacggcca
      421 tcggctcgcc tcgcttctgc atctccagca gcagatcgtc ggcgggcttg ctggcgggca
      481 cgtagaggag ctgccggagg gccgtgcgga cgggctgggc ggccgaggcg ggcagctgct
      541 tcaggatatc gaaggcattg accacgccga cgatgtcgat gatatcgccc cggtagacgg
      601 gcagccggga gaagcccgtc tcgttgatga gtcgagcggc atcgccaaag ttcgcctcgt
      661 cctcgagggc cgccacattg gagatcggca ccatgacccg gcccgcgctc gcctcggaga
      721 aatcaaagat gcgctccacc atgactttct ccgagcgctt gacatcggtg tccgccgctt
      781 cctccagcag aagatacttg agaccgtcct tggtgatgta gggcagggag agcttccccc
      841 gttcgcctgc caggacatag acggcgccgc gggcgatctt cgagatcaaa aacaccaggg
      901 ggtagagaac ccatgaggcc acccagagga agggggcgat ggtcatggcc cacttctcgg
      961 cgtgctgcct gcaaatgctc ttgggaagga tctcaccgaa tatgaggaca atgggcacca
     1021 tgatggccac ggagatccac tcgccgcggg ccggtccgaa atgaccgatg agcagggatg
     1081 tggccaggac cgtgctggtg gtgacgcaga ggacggttcc cgtaagcgtc gtcgcgagaa
     1141 accactccgg tcgatccagg aggcgcagtg cgagtcgtgc cgacgggatt ccatcctttt
     1201 cccttggccg gatgatgttc ggatccgatg ccagaaaagc aatctcgccc cccgagaaga
     1261 tcccctccag agcgatgcag agcggaatga gcatcaggat ccagagatca ctcatggccc
     1321 tcccctttcc ccagcatcac ccggacattg aggatccgcg tccccttgac cttgcgaacc
     1381 acgaagctca gtccctcgta ggaaatcccg tcacccctcg cgggcagcct gccgaagaga
     1441 tggaagacaa acccgccgac ggtcccgaaa tcctccgagg gaatggatgt cccggcgatg
     1501 ctgttaaagt catcgatgga catggccccg gagaccatca ggttttgctc gtcgatccgg
     1561 tggaagggcc tctcgcgggt gtcccgttcg tcgtagatat ccccgaagag ggcctccagg
     1621 atgtcctcca tggtcacgag gcccgagatc ccgccgtact cgtccacgac gagggccacg
     1681 tgcgtgtttc gcttctggaa atcccgaagc acctggtcgg cactgcgttc ctccggaacg
     1741 aaatagggct tgcgcagaag cggctccgtc gaagccgggg ccttcccctc cgccatcagg
     1801 cccagcaggt cgcgcgcata gagaatcccg acgatatcat cggggcccgt cccgtacagg
     1861 gggatccgcg agtagccctg ctcgatgatc tccctgcgca gagcctgcgg gctcgtggac
     1921 acgggcaggc agaacatgtc gacccgcggg gtcatgat
//
LOCUS       DATGDI010000197         8267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_253923, whole genome shotgun sequence.
ACCESSION   DATGDI010000197 DATGDI010000000
VERSION     DATGDI010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253923"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VKO45_07230"
     CDS             <1..426
                     /locus_tag="VKO45_07230"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HMA05704.1"
                     /translation="MIREMKKASLTLGSTLGLSGLSLVIWATDIATTLSMAAMFQAEI
                     GLAVVVLAVVVGNLAKAVPVTPGGIGTYELAVAVILVLAGTTDQASATLIAVTDHLVK
                     NIITAVGGILSTRLLGDWVVDAIRDAFHGKGAAEGPHGG"
     gene            416..2413
                     /locus_tag="VKO45_07235"
     CDS             416..2413
                     /locus_tag="VKO45_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HMA05705.1"
                     /translation="MEAELQFLYVLRWLVLVKFLQLSLYPILRPRFGRFAYPSSYPAG
                     ILAFVALTWYLGLLRLPIALAVLPFLALLAWALFRGRYHLADLKPDLRWDLVFLLAFL
                     ALLLVRFANPAILSAEKFMDHAFIASIMRSPVVPPPDPWYAGGTMNIYYYGGYWMMGV
                     LGIVSGVPSSVVFNLALPTIFALAAVGLYATGELLLPRFRWFPLLTLVLVNPDIIREL
                     ILGGSILWNSTRVITDTINEYTLFSFLWGDAHPHVLDIANQVFFILLLFIALKCWYEL
                     GKGGKITVIALASLSLGSMPVMNTWDVLVYAPLGVLFGLLILWDGFRREARDLSALGF
                     LAGVPILSILLYLPYYLQLAATGFLGIYPVTTPSDPVEFLLVHGFFLAVFLAYGARGL
                     AKRPYLLLPAVLVAVAGYPAAAIAVAAGTALISRRNRDPEILIALAGLAIISLCEVLY
                     FKDYLGGIYYRMNTIFKFYNIAWILMGVSSLVIIARVLDGARFPRISSGEVKAAALIT
                     MAILIVAVPPILASGIGTGPMTLDGLQYLETTHPGDAAALPFVRGLPPGTVIVEGAKG
                     DYAYPSRISSFTGVQTIIGWPGHEFMWRGTGGRTPARIDEVRAIYEDPARAPGILRGY
                     NATYVYVGDTERELYSRLNLPLKNLEPVYEKQGVVIYRVTG"
     gene            2546..2914
                     /gene="rpl7ae"
                     /locus_tag="VKO45_07240"
     CDS             2546..2914
                     /gene="rpl7ae"
                     /locus_tag="VKO45_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HMA05706.1"
                     /translation="MATGFKKFEVPDDIQNKALEALEIARDTGKIKKGSNEATKAIER
                     GIALLVLIGSDVQPEEVIMHLPALCDEKKIPYLFVKRQNDIGAASGLDVGSAAAAIVK
                     PGKAKEIIDDIAKKVAELKG"
     gene            2922..3131
                     /locus_tag="VKO45_07245"
     CDS             2922..3131
                     /locus_tag="VKO45_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HMA05707.1"
                     /translation="MAGDATPAEVIEVIGSTGMHGEAMQVKCRILDGPNKGRIITRNT
                     VGPIREGDILMLLETEREAKKLSKR"
     gene            3136..3327
                     /locus_tag="VKO45_07250"
     CDS             3136..3327
                     /locus_tag="VKO45_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HMA05708.1"
                     /translation="MVDMRTCSFCGENIDPGTGKMFIRRDGTIFYFCSSKCQNNARLG
                     RVPRRVRWTQAGRKALGKE"
     gene            3333..3791
                     /gene="ndk"
                     /locus_tag="VKO45_07255"
     CDS             3333..3791
                     /gene="ndk"
                     /locus_tag="VKO45_07255"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HMA05709.1"
                     /translation="MDRTFVLIKPDGIQRGLAGEIISRLETKGLKLVGAKFLLPTESQ
                     VMEQYEEHVGKPFFPQLKKYIMSGPVLAMVWEGRDAVPVVRRVVGATNPAEAAPGTIR
                     GDLALDTGRNVIHASDSPASAAREAAIYFSEKDLVSYPRIDEPVLYEWVG"
     gene            4054..4278
                     /locus_tag="VKO45_07260"
     CDS             4054..4278
                     /locus_tag="VKO45_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05710.1"
                     /translation="MGYDRSEILLIAITLCFVIGGSIFAVMVVGAEYGVLEVPYGPYI
                     LLVLAVIIVILAGLMVITGTKKDEKQAGSV"
     gene            4295..5092
                     /locus_tag="VKO45_07265"
     CDS             4295..5092
                     /locus_tag="VKO45_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HMA05711.1"
                     /translation="MRICSAQMASAWEDPGANLEKAESCIASAKEMGADLVCFPEQYS
                     TGWSPHSTRFAEDPDGPTVQALRDLARRRSIFVIGAYVERHEPRPLNTSVAIDPRGEV
                     VASFSKIHPFSPGGEDLHYDPGDHLSIFMAGRVRFGIAICYDLRFAPLFRAYALAGVH
                     AVLVPSAWPCSRIHAWEILVQARAIDNQFYVVGCNPTGTTPVDRYCGHSLSADPRGAV
                     ISRGGEGEELVMTDIDPAKVDEARTRIPVEKDRRPDLYHRLFSDTGA"
     gene            5166..6374
                     /gene="thrC"
                     /locus_tag="VKO45_07270"
     CDS             5166..6374
                     /gene="thrC"
                     /locus_tag="VKO45_07270"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HMA05712.1"
                     /translation="MSYQLACISCGATYRPEEILYSCRACGHPLEVRYRLGELGIDRK
                     AWNRRPLSVWRYREILPVQREPVTLREGGTPLYHLEALGKELGLPRLYAKHEGMNPSG
                     SFKDRGMTVGVSMALQLGKRSVACASTGNTSASLAMYAAKAGIPAVVLLPSGKVALGK
                     VAQALIHGARAITIRGNFDRALEMVQELCEKHGLYLLNSMNPYRLEGQKTIGFEVLDQ
                     LGEVPDRIVLPVGNAGNISAIHKGLREFRELGFIDRLPMMTGVQAEGSAPVVRAVREN
                     LPEVIPEAHPETVATAIRIGAPVNAEKALRAIRTTGGTAVSVSDAEILSMQRDLASRE
                     GVGVEPASAASVAGVKKLVADGVIDRNERVVCVVTGHLLKDPETVVRQCEPPLEIEPD
                     LPSLLSALRL"
     gene            6371..6934
                     /locus_tag="VKO45_07275"
     CDS             6371..6934
                     /locus_tag="VKO45_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05713.1"
                     /translation="MICTAGAGAANASFQVSPGGASYSAAVEVTEVSGHGFWDIGPLG
                     ERIPRPVSNVSLHGTCGNCSFNWIDPFTMNFTEGNYTILYTGQILENHLQGSFDGPYH
                     VSVGLPPGLDVRDPLLGMRSPGSEIEANDSSLTIVWNGTTSFELRFYTPERERLLLAF
                     GALWLFGLVLVLIPYLLSRRAGERKEP"
     gene            6931..7509
                     /locus_tag="VKO45_07280"
     CDS             6931..7509
                     /locus_tag="VKO45_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HMA05714.1"
                     /translation="MIEVLIASFLVGLTGAVSPGPMSASILGMSHKKPLLSTAGLVAG
                     HGIVEAAIIVGIIFGLSSLPYQTEIALLGCVTIVILGMLQFLAEPEQKAARAGGAGAP
                     FVAGIVLTLSNPYWWIWWLTFGAGFLALQPAYAAFFIGHISADLVWFGILALTASRGA
                     KILGDRYRWVVKGSGVAMAAFGILFAYSILSG"
     gene            complement(7524..>8267)
                     /locus_tag="VKO45_07285"
     CDS             complement(7524..>8267)
                     /locus_tag="VKO45_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMA05715.1"
                     /translation="ENAAAVGSGVEAALRKQGRSEKVVIIGGDGATFDIGMLCISGAF
                     ERGHDFTYICYDNEAYMNTGIQRSGATPLDASTTTSPAGKCSTGNRRPKKDMPAILAA
                     HGAPYVATTSIAYPTDLMQKVERAIETKGPCYVQILAPCPTGWGYEGDRTMVIGKLAV
                     ETGLFVLFEQTDGTVTKVKKVMRKPVEEYLKVQKRFAHLFKPQRNETEIARIQAIADA
                     NAAKFGIDIKLKKPAEKPESEAGIQKPPA"
BASE COUNT         1322 a         2812 c         2581 g         1552 t
ORIGIN      
        1 atgatccggg agatgaagaa agcctccctc accctcggct ccaccctcgg cctctccggc
       61 ctctccctcg tcatctgggc gacggacatc gcgaccaccc tctccatggc ggcgatgttc
      121 caggcggaga tcggcctcgc cgtggtggtc ctcgccgtcg tggtgggcaa cctcgcgaag
      181 gccgtccccg tcaccccggg agggatcggg acctacgagc tggccgtcgc ggtcatcctc
      241 gtcctcgcgg ggaccaccga ccaggcttcg gcgaccctca tcgccgtcac cgatcacctg
      301 gtgaagaaca tcatcaccgc cgtcggcggg atcctctcca cacggctcct cggggactgg
      361 gtcgtggacg ccatccggga cgccttccac gggaagggag ccgcggaggg accgcatgga
      421 ggctgagctc cagttcctgt atgttctacg ctggctggtc ctcgtcaagt ttctccagct
      481 gtccctctac ccgatcctca ggccccgctt cgggaggttc gcctacccct cctcctaccc
      541 ggcagggatc ctcgccttcg tggcactcac ctggtacctc ggccttcttc ggctccccat
      601 cgccctcgcc gttctcccgt tcctcgcgct cctcgcctgg gccctgttcc ggggccgtta
      661 tcacctggcg gacctgaaac cggacctgcg gtgggacctc gtcttcctcc tcgcctttct
      721 cgccctgctt ctggtcaggt tcgcgaaccc ggccatcctc agcgccgaga agttcatgga
      781 ccatgccttc atcgcctcca tcatgcgaag ccccgtggtc ccgccccccg atccctggta
      841 cgccgggggg accatgaaca tctactacta cggcgggtac tggatgatgg gggtgctcgg
      901 catcgtgagc ggcgtcccct cgtccgtggt cttcaacctc gccctcccga cgatctttgc
      961 cctcgccgcc gtgggcctct atgccaccgg cgagcttctc ctcccccggt tccgctggtt
     1021 ccccctcctc acccttgtcc tcgtgaaccc cgatatcatc cgggaactga tccttggcgg
     1081 gagcatcctc tggaacagca cccgggtcat cacggatacc atcaacgagt acaccctctt
     1141 ctccttcctc tggggggatg cccaccccca cgtgctcgac atcgcgaacc aggtcttttt
     1201 catcctcctc ctgttcatcg ccctgaaatg ctggtatgag ctggggaagg gagggaagat
     1261 caccgtgatc gcccttgcct ccctctccct gggctcgatg ccggtgatga acacctggga
     1321 tgtcctcgtg tatgcgccgc tcggggtcct cttcggcctg ctcatcctgt gggatggctt
     1381 ccgacgggag gcacgggacc tctcggccct gggattcctc gccggtgtcc ccatcctttc
     1441 catccttctg tacctcccgt actacctcca gctggcggcc accggtttcc tggggatcta
     1501 cccggtcacc accccttccg atcccgtcga gttcctcctg gtccatgggt tcttcctcgc
     1561 cgtgtttctc gcatacgggg cgaggggcct cgccaaacgg ccctacctcc tccttccggc
     1621 ggtgcttgtc gccgtcgcgg ggtacccggc ggcggccatc gccgttgccg cgggcaccgc
     1681 cctcatctcc cggcgaaacc gggatccgga gatcctcatc gccctcgccg gcctcgccat
     1741 catcagcctc tgcgaggtgt tgtacttcaa ggactacctc ggcgggattt actaccggat
     1801 gaacaccatc ttcaagttct acaacatcgc ctggatcctc atgggggtct cgtccctcgt
     1861 catcatcgcc cgggtcctgg atggcgccag gttccccaga atttccagcg gagaggtgaa
     1921 ggccgcggcc ctgattacga tggccatcct cattgtcgcc gtccctccca tcctcgcctc
     1981 gggcatcggt acgggcccca tgaccctgga cggtctccag tacctggaaa ccacgcatcc
     2041 cggcgatgcg gccgccctcc ccttcgtccg ggggctcccg ccgggaacgg tgattgtcga
     2101 gggggcgaag ggggactacg cgtacccctc ccggatctcc tccttcaccg gtgtccagac
     2161 catcatcggg tggcctggcc atgagttcat gtggaggggg accgggggga ggaccccggc
     2221 acggatagac gaggtgaggg caatctacga ggacccggcc cgggctccgg gtatcctgag
     2281 aggctacaac gcaacttatg tctatgtcgg ggataccgag cgggagctgt acagccggct
     2341 gaaccttccc ctcaagaacc tcgagcctgt ttacgagaag cagggggtcg tgatctaccg
     2401 ggtcaccggc tgaaggccat ccggaacacg ccgggggagc cgtccccgcg acaaaccttt
     2461 atctcccgcc ctgatgacat ggtatggtta cggcgtcact acctgtgagt gatgaaggag
     2521 aattccctcc tgaatgaggt gtgttatggc aacgggattc aagaagttcg aggtccccga
     2581 cgacatccag aacaaggccc tcgaggccct ggagatcgcc cgggacacgg ggaagatcaa
     2641 gaagggttca aacgaggcca cgaaggccat cgagcgcggc atcgccctcc ttgtcctcat
     2701 cgggagcgac gtgcagcccg aggaggtcat catgcacctc cccgctctct gcgacgaaaa
     2761 gaagatcccc tatctcttcg tgaagcggca gaacgacatc ggcgctgcaa gcggcctcga
     2821 tgtcggttcg gcggcggcgg ccatcgtgaa gccggggaag gcgaaggaga tcatcgacga
     2881 catcgcgaag aaggtcgccg agctcaaggg atgaagggtt catggcggga gacgcgacac
     2941 cggccgaggt catcgaggtc atcgggagca ccggcatgca cggtgaggcg atgcaggtga
     3001 agtgccggat cctggacggg ccgaacaagg ggaggatcat cacccggaac accgtcggcc
     3061 ccatccgcga gggcgacatc ctcatgctcc tcgagaccga gcgcgaggcg aagaagctct
     3121 cgaagaggta agcccatggt agacatgcgc acatgcagtt tctgcgggga gaacatcgac
     3181 ccgggcacag ggaagatgtt catccggcgg gacgggacca ttttctactt ctgcagctcc
     3241 aagtgccaga acaacgcccg gctcgggagg gttccccgcc gggtccggtg gacccaggcg
     3301 ggaaggaagg ccctcgggaa ggagtgatcc gggtggatcg cacctttgtc ctcatcaagc
     3361 ctgacggcat ccagcggggc ctcgcgggcg agatcatctc ccggctggag accaaaggcc
     3421 tgaagctcgt gggtgcgaag ttcctcctcc ccaccgagtc ccaggtgatg gagcagtatg
     3481 aggagcacgt cgggaagccc ttcttccccc agctcaagaa gtacatcatg agcgggccgg
     3541 tcctcgccat ggtctgggag gggcgggatg cggtcccggt ggtgcggagg gtggtcgggg
     3601 cgacgaaccc ggccgaagcg gctcccggga ccatccgggg cgatctcgcc cttgacaccg
     3661 ggaggaacgt gatccatgcc tccgactccc cggcgagcgc ggcccgggag gcggccatct
     3721 acttctccga gaaggacctc gtcagctacc cgaggatcga tgaaccggtc ctctacgaat
     3781 gggtcggctg agatcccggg tcccgcggag ccgggatcgt ccagggggga aggggcccct
     3841 ctggacggga tcccctcctg cctccttcac tttcaccccc cgcactgcca tgccttatta
     3901 tccccggggg ggacggtttc cctgcatacc attatcccac ctcttttcgg gaggagagcc
     3961 ccgtgctccc cttcatcaac tatttaactt tgcgtgccaa gatagagaat aaggccggcc
     4021 ctgcgggtcc ggatcctccc ggggaacgaa ttcatgggat acgacaggag cgagattctc
     4081 ctcatcgcca tcaccctctg cttcgtcatc gggggatcca tcttcgccgt gatggtggtg
     4141 ggggcggaat acggggtcct cgaggtgccg tacggaccct acatcctcct cgtcctcgcc
     4201 gtaatcatcg tgatcctcgc cgggctcatg gtcatcaccg gtacgaagaa ggatgagaaa
     4261 caggcgggct ctgtctgacc cgcagcatcc accgatgagg atctgttctg cccagatggc
     4321 gagcgcgtgg gaagatcccg gcgcgaatct cgagaaggcc gaatcctgca tcgcctccgc
     4381 gaaggagatg ggtgcggacc ttgtctgttt ccccgagcag tattcgacag gctggtcacc
     4441 ccattccacc cggttcgcgg aggatccgga cggcccgacc gtgcaggccc tccgggatct
     4501 cgcccgaagg cgctccatct ttgtcatcgg ggcatacgtg gagaggcacg aaccccggcc
     4561 cctgaacacc tccgttgcca tcgatccccg gggcgaggtg gtggcgagct tctcgaagat
     4621 ccatcccttt tcccccggcg gggaggacct ccactatgat cccggcgacc atctgtccat
     4681 cttcatggcg gggcgcgtgc ggttcggcat cgccatctgc tacgacctcc gattcgcccc
     4741 cctcttccgg gcctatgcgc tggcgggagt ccacgctgtc ctcgtcccgt cggcctggcc
     4801 ctgctcccgg atccatgcct gggagatcct cgtccaggcc cgcgccatcg acaaccagtt
     4861 ctacgtcgtc ggctgcaacc ccacgggcac cactcccgtg gaccggtact gcggccactc
     4921 cctctcggcc gatccccgag gggccgtgat cagccggggt ggggagggcg aggagctcgt
     4981 gatgaccgat atcgacccgg cgaaggtcga tgaggcaagg acccggatac cggtggagaa
     5041 ggaccggcgg ccggacctgt accaccggct cttctcagac accggggcct gagccccctc
     5101 cttccctccc tgaacggggt tctggccgaa tagatatacg tgccggggcc gacaatgtac
     5161 tcacaatgtc ctaccagctg gcctgtatct cctgcggagc tacatacagg cctgaggaga
     5221 tcctctactc ctgccgggcc tgcgggcatc ctctcgaggt acggtaccgg ctcggagagc
     5281 tcgggatcga tcggaaggcg tggaacaggc gcccgctctc ggtctggcgc taccgcgaga
     5341 tcctgccggt gcagagagag cccgtgaccc tccgggaggg gggaaccccg ctctaccacc
     5401 tggaggctct cgggaaggag ctcggccttc cccggctcta tgccaagcac gaggggatga
     5461 acccctccgg ttccttcaag gaccggggga tgaccgtcgg ggtgtccatg gcactccagc
     5521 tggggaagcg ctcggtggcc tgcgcgagca ccgggaacac ctcggcctcc ctcgccatgt
     5581 acgcggcaaa ggcgggaata ccggccgtgg tcctcctccc gtcgggcaag gtcgccctcg
     5641 ggaaggtggc ccaggccctc atccacgggg cccgggccat caccatccgg gggaacttcg
     5701 accgtgccct ggagatggtc caggagctct gcgagaagca tggcctctac ctcctcaact
     5761 ccatgaaccc ctaccggctc gagggccaga agaccatcgg cttcgaggtc ctcgaccagc
     5821 tgggcgaggt tccggaccgg atcgtgctcc ccgtcgggaa cgccgggaac atctcggcca
     5881 tccacaaggg tctcagggag ttccgggagc tcggcttcat cgaccggctt cccatgatga
     5941 caggggtcca ggcggaagga tcggcgccgg tggtgagggc cgtccgggag aacctcccgg
     6001 aggtcatccc cgaggcgcac ccggagaccg tcgccaccgc catccgcatc ggtgccccgg
     6061 tgaacgccga gaaggctctc agggccatcc gcactacggg agggacggcc gtctcggtga
     6121 gcgatgccga gatcctctcg atgcagcggg atctcgcctc ccgggagggg gtcggggtgg
     6181 agcccgcctc ggccgcgtcg gtggcggggg tgaagaagct cgtcgcggac ggggtcatcg
     6241 accggaatga acgggtggtc tgcgtggtca cgggccacct cctgaaggac ccggagacgg
     6301 tggtgagaca gtgcgagccc cctctcgaga tcgagccgga cctgccctcg ctgctctctg
     6361 cgctgcgatt gtgatctgca ccgcgggggc gggggccgcg aacgcgagct tccaggtctc
     6421 ccccggaggc gcttcctact cggcggctgt cgaggtgacg gaggtctcgg ggcacggctt
     6481 ctgggacatc ggacccctcg gggagcggat cccccggccg gtctcgaacg tgagccttca
     6541 cggcacctgc ggcaactgca gcttcaactg gatcgatccc tttaccatga acttcaccga
     6601 gggcaactat accatcctct acaccggcca gatcctggag aaccacctgc agggcagctt
     6661 cgatggcccc taccatgtct cggtcggcct tcctcccggc ctggacgtgc gggatcccct
     6721 cctcggcatg aggagcccgg gctcggagat cgaggcgaat gactcgtccc tcaccatcgt
     6781 gtggaacggc acgacctcct tcgagctccg gttctacacc cccgagaggg agagactcct
     6841 cctcgccttc ggggccctct ggctcttcgg actcgtcctc gtgctcatcc cgtatctcct
     6901 gtcccggagg gcaggggaga ggaaggaacc gtgatcgagg tcctcatcgc ctccttcctt
     6961 gtcgggctga cgggagccgt ttcgccggga cccatgagcg cctccatcct cgggatgtcc
     7021 cacaaaaagc ccctcctctc caccgcgggg ctcgtggccg gtcatgggat cgtggaggcc
     7081 gccatcatcg tcgggatcat cttcggcctc agctccctcc cctaccagac cgagatcgcg
     7141 ctcctcgggt gcgtcaccat cgttatcctc gggatgctgc agttcttggc cgagccggag
     7201 cagaaggcgg cccgggccgg gggtgcgggt gcccccttcg tcgcggggat cgtcctcacc
     7261 ctctcgaacc cgtactggtg gatctggtgg ctcacctttg gtgcggggtt cctcgccctc
     7321 cagccggcct acgcggcctt tttcatcggg catatctccg ccgatcttgt ctggttcggt
     7381 atcctcgccc tcacggcctc ccggggtgca aagatcctcg gagacaggta ccggtgggtg
     7441 gtgaagggga gcggggtcgc gatggctgcc ttcggcatcc tcttcgcgta ctcgattttg
     7501 tcgggctgag gtggaaaaaa agatcaggcg ggcggctttt gaattcccgc ctcactttca
     7561 ggcttctccg ccggtttctt caatttgatg tcgatcccga acttcgccgc gttcgcatcg
     7621 gcgatggcct ggatccgggc gatctcggtc tcgttccgct ggggcttgaa gaggtgggcg
     7681 aaccgcttct ggaccttcag gtactcctcg acgggctttc gcatcacctt cttcaccttc
     7741 gtgacggtcc cgtcggtctg ctcgaagagg acgaagagtc ctgtctccac ggcaagcttc
     7801 ccgatgacca tggtccggtc gccctcgtag ccccacccgg tggggcacgg ggccaggatc
     7861 tggacgtagc agggcccctt ggtctcgatg gcccgctcca ccttctgcat caggtcggta
     7921 ggataggcga tggaggtggt ggcgacgtaa ggggccccgt gggcggcgag gatggccggc
     7981 atgtccttct tgggccgccg gttccccgtg gagcacttcc cggccgggga ggtcgtggtg
     8041 cttgcgtcga ggggagtcgc gcccgagcgc tggataccgg tgttcatgta ggcctcgttg
     8101 tcgtagcaga tgtaggtgaa gtcatggccg cgctcgaagg ccccgctgat gcagagcatc
     8161 ccgatgtcga aggtcgcccc gtcgcccccg atgatcacca ccttctcgct tctgccctgc
     8221 ttcctgagcg ccgcctcgac cccggagccc accgccgccg cgttctc
//
LOCUS       DATGDI010000198        29059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_79142, whole genome shotgun sequence.
ACCESSION   DATGDI010000198 DATGDI010000000
VERSION     DATGDI010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 29059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29059
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79142"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /locus_tag="VKO45_07290"
     CDS             <1..1838
                     /locus_tag="VKO45_07290"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMA05716.1"
                     /translation="GYVDDRKDCDDTNPAINPLGTEVCDGKDNDCDGQVDEGLRQTFY
                     WDRDRDGVGGSDTIQACTQPPGAVTSTGDCNDNDWWVYPGATEYCDGKDNDCDGAVDE
                     PDGLVCRFADKGDGTVLDTATGLYWLKKADLGGGTMTLAETRALVKNVADGNNGLSDG
                     SWAGAWRLPTIDEMSGLCGYRCGGPGGLCSLGNAAGIGPWREGDAFSRVPVNPSNVYW
                     TDTTADSGRQRLFAVYGGYCLGLPWLSAEPDWPFYYSYHAWPVREALVDNDGDGYHDL
                     AGPRVGEDCDDTNPSVNPGMTESCNGIDDDCDGRVDETCDYDFDGFTPEQGDCEDRNA
                     SINPSQEEEINGIDDNCDGHIDERFTDKGDGTVLDNLRHIRWLKDPSGIPPRTWLEAV
                     NTVAALGDGQYGLSDGSKPGDWKLPTVAEFNTLADPRFAYDQWGDCQCEVLDLPKYYR
                     KCWLEVPTGPALSNSQGNAHWSEGDPFLDVDELNTACDGDCQRLCQHQYISSGGFVIA
                     IPWGECVGQTQVCNLNLFWTRETVGAYLGVDWSELTDPKSTRECRSCGSVWGNVIQGY
                     WGDWPWSDPGGTCCVTYDINRGTPPDYWPGYDCNQKLKVWPWRPA"
     gene            complement(2030..2101)
                     /locus_tag="VKO45_07295"
     tRNA            complement(2030..2101)
                     /locus_tag="VKO45_07295"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2067..2069),aa:Thr,seq:cgt)
     gene            2221..2415
                     /locus_tag="VKO45_07300"
     CDS             2221..2415
                     /locus_tag="VKO45_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05717.1"
                     /translation="MQRLTASERLPGGITVYWEEKGEEEETSFSFMELIDRRINSLDL
                     LEHPAKYVFDNDTGHLILKP"
     gene            2499..2681
                     /locus_tag="VKO45_07305"
     CDS             2499..2681
                     /locus_tag="VKO45_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05718.1"
                     /translation="MRTERCGDGVNVVWKKAGKDTTSYFSFTQLIDMQVNALDLLAHP
                     QSWGMDEKKGKVVLVV"
     gene            2714..2935
                     /locus_tag="VKO45_07310"
     CDS             2714..2935
                     /locus_tag="VKO45_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05719.1"
                     /translation="MQPILRTERRGDGVNVVWEMAGEEMNSYFTFAELIDLRVNALDL
                     LNTPSNYGIDEKTGKVGFVAFCHLSRRHE"
     gene            complement(2926..3222)
                     /locus_tag="VKO45_07315"
     CDS             complement(2926..3222)
                     /locus_tag="VKO45_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HMA05720.1"
                     /translation="MNPPREIQVQIPQDLDPVYSNRIQIAYKEDEFTFIFLHEIPGTN
                     QARAKAIVSISPRHAKNFLEVLGTSMADYEGKFGKIETPKEKQPVENVTMRGYS"
     gene            3378..4049
                     /locus_tag="VKO45_07320"
     CDS             3378..4049
                     /locus_tag="VKO45_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HMA05721.1"
                     /translation="MPRTLVSVSEIVRCHFCPVRFSLERKLEARESPRYTIAKQVSSH
                     LGRDLDEDGIWEEVEAILPEIDPAYRSYLSASVERCRAGAWRIPSETEVPVRSDRFGI
                     RGLVDKIFDSEPTFAITRPTESPAIGVSTSDRIRVAAYALCIRESLGIPASRGAVEYI
                     PSGEVRICTPQPRDRRAVIRAAEAVRYVREGHIPRRPLSAPCGGCPHRERCGTGAKKL
                     SDLLG"
     gene            complement(4060..4989)
                     /locus_tag="VKO45_07325"
     CDS             complement(4060..4989)
                     /locus_tag="VKO45_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase subunit beta"
                     /protein_id="HMA05722.1"
                     /translation="MKPLRLRDFIEDRDGWIYSVATYDNADRVGCILRYVPDPSGDRV
                     NPEGVRYRKAGFEEAFDLVRQKKPSYTGRFLRIPGKDVRRVYKPEERLDFCAGRDRRV
                     RALVDLFALPEGALGCTGSRLLGMESPASDIDLVAYGSAFPVAQETLRRETERGSLAG
                     LSEEMWRTVYTKREPEIPFESFVLHEKRKWNRGVIGGTYFDLLFTRAYDDLDGRPVPE
                     GERIRRMTIEARVTDASLSFDSPAVYLVDHEVVNRIVSFTHTYSGQALPGETVEASGV
                     LEGHGAERWLVVGTTRVAKGEYIVSKTLLEGSG"
     gene            complement(5037..5615)
                     /locus_tag="VKO45_07330"
     CDS             complement(5037..5615)
                     /locus_tag="VKO45_07330"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HMA05723.1"
                     /translation="MDIVERAIQVLRRDGIVVYPTDTVYGLGGDAFSDGAILRVYEAK
                     HRPLGNPVSVAVSDVEMLCAIACADEYDLKFIDRFLPGPFTVILGARSCIPDMLTGGT
                     GRIGVRIPAHDLALAIIRGFDSPITATSANVSGEKDPVSPSEVRVPYDLLVDGGFLPG
                     TPSTVVDLVEKQILRRGTRAEEVEEYLRGQGA"
     gene            complement(5618..6292)
                     /locus_tag="VKO45_07335"
     CDS             complement(5618..6292)
                     /locus_tag="VKO45_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HMA05724.1"
                     /translation="METPEIYGISITCEHRPGVLRDIAGVLAAHDANIVIVQQSLIPS
                     GPATGCASLYFELEGGDDRGKLMADLLNLPSVRDVVTYASFSRIFGSRVIIIGGGAQV
                     AQVALGAVNEADRHNIRGERISVDTIPLVGEEDLAQAVDAVVRLPRAQILVLAGSLMG
                     GKISEAVDRVREAGIPVIALKMAGSVPKHADLVVTDPIQAGVFAVMHVSRYASFDIGR
                     VRGEEF"
     gene            complement(6303..6373)
                     /locus_tag="VKO45_07340"
     tRNA            complement(6303..6373)
                     /locus_tag="VKO45_07340"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6339..6341),aa:Gly,seq:ccc)
     gene            complement(6421..7902)
                     /locus_tag="VKO45_07345"
     CDS             complement(6421..7902)
                     /locus_tag="VKO45_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMA05725.1"
                     /translation="MLGKYSDEDITRVLWAVAIPVTSLLAIAVTIVSVTREITDVFPH
                     LYYIPILLNAYRNPSKAIGFAAFLGAIYAGLVYFLSGYAVDPLFRAFVRLLVFIWVAA
                     IISYLAGNLLRRNRHLSAMNEIIAAASTSASLDEILSTAGRRIREMLGSAAVMVYLVS
                     DDGRQGSLRYHEGLSDEAAGRSREIDLSVEPFRGILAGGLPVLTGEKNSFGDLNLPGV
                     LSFVLIPLPAGSQALGLIAAGRGKAAPLAEDEMAMLESVSRAIGSAVKKTLLQEELGL
                     ANTKANLYLDIMSHDINNVNTVSLGYAQLLADLVTGKSREMLAKLESAVQQSVEIIRN
                     VSTIRAIQERSQATRAVNLDPVIRMELDHHPEVTIHYDGTTVSVCANSLLSEVFRNLI
                     GNSAKFGGTGAQVWIRVAEDGDRVTVSVEDSGPGIPDDLKPAIFNRFQRGRSSRSGKG
                     LGLYISRMIIEGYGGRIWVADRVEGEPGKGAAIRFTLPKGCTA"
     gene            8245..8703
                     /locus_tag="VKO45_07350"
     CDS             8245..8703
                     /locus_tag="VKO45_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HMA05726.1"
                     /translation="MARMHARRRGKAGSVRPYRKEPPAWSNTDVKEIEKVVREERRQG
                     RSAAEIGLLLRDKFGVPDVKLATGKRLGQMLREMGMESKIPDDLRNLIEKALGLRKHL
                     STNKNDLHNKRQLQLIESKVRRLVKYYIRSGTLPQDWVYKPETAEILLSR"
     gene            8708..9922
                     /locus_tag="VKO45_07355"
     CDS             8708..9922
                     /locus_tag="VKO45_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HMA05727.1"
                     /translation="MALHADAEQLAGHLLKQEFVEIHAHHDADGIAAGAIIATAMHRS
                     GRSFRLRCSPRISPADLAGEGGVLLCDFGASRNDLPPHVMVIDHHPPRFGGEFHVNPA
                     RSGDGTGGEVAASGAAYLVAQHMGENRDLAGLALIGMVGDRQDCTGANREILNDGIAN
                     GFITPGRGFSLAGRDPVESLSLSTRPYLDGISGDEAAAQRLVAECERDGKLSLDALLS
                     LVILRSGSSLSAPALESLYGDTYTLEREAVPDLHSLAALVDACGRAGMGGLAASLLIS
                     RRPAGTDAWEVLRSYRARVIAGIRSAQPLETDGWYGIGDASVNGPVADALVLSREGKG
                     PVFVFAPGDGVWHVSARCPPGVRMDLEKIVRDLALASGGDGGGHRCRAGATIGADHLD
                     RFRKGLAEAVAS"
     gene            9919..10143
                     /locus_tag="VKO45_07360"
     CDS             9919..10143
                     /locus_tag="VKO45_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HMA05728.1"
                     /translation="MISVRGTVTTSHRDPARVAGSLSPDNLSSMSMSPGEGIVVMEIS
                     GGNLRSIIASVDDYLMNLAIAEDVCTCTSP"
     gene            10122..11636
                     /locus_tag="VKO45_07365"
     CDS             10122..11636
                     /locus_tag="VKO45_07365"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HMA05729.1"
                     /translation="MHVHFSLKADIIFSGDIGTEARASVDRAVTEANEKVFPKGVPKG
                     VPPEEVGRITRWEVEGNRLALEIESGGYTRAHDALFRFRKLIGAALGKHQLGLREIGV
                     REFMAGITGDIPETAVPRLPFIRGVSREAGCVFLDLAVTTQDLENRIPDRIVRLIEEK
                     VEAAGYGGKTEHWELMYESPKKPRVYTGNPTEEMEKRHWIRHGSSRGQWIYAPQPIQL
                     FRAFTSLVEEEIIRPLGFSEMVFPKLDTWEVLSRSGHAKGVYPEIYYVCTPKTRDPAF
                     WEDVADYFKVTGVVPVEMLRERIDGPIGALCYAQCPSFWPFVQGRTVATSSLPVRIFD
                     RSGTSHRYESGGIHGIERVDEFHRIELVWFGTVEQTTKMAEELHRAYTRVFNELLELE
                     WRAAKVTPWFMAQEGMVGACGCESVGTTDYEAFLPYSGSWLEFQNVSVNGDKYPKGFN
                     VKIQNGEECWSGCSGIGLERWTAAFLAQKGFDPAEWPGAVRERVGTVNEVMRFL"
     gene            11650..12348
                     /locus_tag="VKO45_07370"
     CDS             11650..12348
                     /locus_tag="VKO45_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HMA05730.1"
                     /translation="MAKKSQIGRKVEGWKAKAWYRIHVPEVFGKSYIGDTISSDPETV
                     MGRILQTTLGEIIQDYTKQHIKIRVRVTSVAGDAAYTEFVGHELTRDYMRSMVKRRTS
                     RIDTVLPVTTKDGKVLRITMTCLTLTRANLSQVHAIRSAMTRFIQSYAAQRELPAFVK
                     DMVSGEITKELFKLAKPIYPLRRVEIIKSRVEPEAKAGEAPPAQPPELTEEKPAPETK
                     PEEKAEVPAPAPAA"
     gene            12635..13867
                     /locus_tag="VKO45_07375"
     CDS             12635..13867
                     /locus_tag="VKO45_07375"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HMA05731.1"
                     /translation="MTAGRVLLLVLDGISDRPCPELGGRTPLEAAETPVLDRLAREGI
                     CGIMDPIGPGIRPGSDTSHLSLLGYPPERYYTGRGPLECEGCGIHMEPGMIGFRANFA
                     TLDKQGKVSDRRAGRIHGTDALCDAIEKEVHLKHGISFRLRPGAGHRAALALRGKDLG
                     ACVSSNDPKTEGIAPPEVKPLTTEARDRRTAEACNDFLGQAHDILARHPLNRNREKEG
                     LPQANAILIRGAGEMGRFEPFAERHRMTGSVIAAATLITGIGKAVGLESIPVPGITGS
                     ADTNLDGKVAAALAELRRKEFVLLNIKGADEAGHDGHPLEKRDFIARVDRALTPLVSL
                     DDTLVVACGDHSTPCTVMDHSADPVPLVIRGEGVRVDRVARFTETACAEGGLHRIPGS
                     ALMPILLDLVNRARKYGA"
     gene            13867..14706
                     /locus_tag="VKO45_07380"
     CDS             13867..14706
                     /locus_tag="VKO45_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05732.1"
                     /translation="MKAAGILDLGEITLLEPTFSSLTEDLYTGALLGIPATGARILID
                     TGENPLAVAVTDGEGWFAANFLFREPAREVIELCERLDAEVHQAERSLWLGAVREYYS
                     LLLAGSVTPALDDLPPDRLPGLTDLLREIWGREEDIPCLDCCCGSGAGSAAARALGMS
                     PLSYDNDPALLSLGLSTGRLLPPETMWIDATQAGRYIGPVPRGIAVMMGEINAFTREM
                     WESITGEFLGLVEEALITVGTEAEARTVETWCRAAGHKPIVSENPRHAFYDRWVVRSG
                     KMQ"
     gene            complement(14738..14956)
                     /locus_tag="VKO45_07385"
     CDS             complement(14738..14956)
                     /locus_tag="VKO45_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05733.1"
                     /translation="MVTVNTMYLDAALDKRLKYISETITPVGIDIQKIIEKQGSSDLF
                     VRKFLERMAHKLPNPMVVLDRLKRIPRP"
     gene            complement(15171..15734)
                     /locus_tag="VKO45_07390"
     CDS             complement(15171..15734)
                     /locus_tag="VKO45_07390"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HMA05734.1"
                     /translation="MPEEMLAETWITLPPALLPAVGVLLMVAGLCLLLWSDVIVSLVI
                     LFLGVLAILLGLGFLAAGHFLGRAGIPPVLLFIAGLASILVGILAFLRRDLVADLIIY
                     LAAGIFVLLGAFLLFIGGLLSSRGWGRRAFLWVGLALLAGGIVLAVFPGEVARAILAA
                     GGALLIVAGAAALLVSYAGRRSSVSRL"
     gene            complement(15795..17804)
                     /locus_tag="VKO45_07395"
     CDS             complement(15795..17804)
                     /locus_tag="VKO45_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMA05735.1"
                     /translation="MRIVVHPQRGYYRLFFLDGGGVTAVGQVDLVGTPKGYRPKDYLV
                     RKPGSRHGRSIPSKDLVATLRRAEVQLTRRDEPWEDFLSDLQIPWSVRTLCRTCLLED
                     RVTPLEPATSVRYGKESVCMDCAKKEVRRELGYFTRFGKVSYAYIESLLAAYRDLDRV
                     LSALQPDGLDLTRTLFDRIDAHPVTETSAVSELPLPPEFRAASGVERLMPVQQLAVGA
                     GLLAGSDLLVVSATASGKTFIGEMAGMKNLLEGRGRMLFLVPLVALANQKYERFTERY
                     GKIAGVSLLTGRNRLNLPENRTRGRQDPDAQVVVGTYEGLDHLVRCGRGLQHIGTVVV
                     DEIQMLEDRDRGHRLDGLIARLKFLAPGAQFLYLSATVGLPHLLAEKLGARLVTYAER
                     PVPLERHLLFVTRKDKIRFEKQLVDAEFSVRSSKGFRGQTIIFTNSRARCHVIAEALG
                     RKAAPYHAGLTAQERRDTERRFAEGKIAAVVTTAALGAGVDFPASQVIFDSLAMGIEW
                     LSVQEFSQMGGRAGRPDFHDRGKVVILAEPGAVYMKEAEATEEEVAIRLLRGEMEEVA
                     PSYGMEESSEEFAAHAVVARGDEEAIGRISRMMVGEAEPVLPELLSRGLVQRRGKSLV
                     LSPLGRVMAEHFIGVERLLEIRRLVGEMEDPVEILAELECTGEER"
     gene            complement(17801..18604)
                     /locus_tag="VKO45_07400"
     CDS             complement(17801..18604)
                     /locus_tag="VKO45_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HMA05736.1"
                     /translation="MGSDDPLATILRSKSEATRFQILVEIAENQPAIRQQEIAGKLGV
                     TPQAVSEYIRELVEEGMVSAQGRGRYEVTREGIEWVVNHAEVLEAYARHIRRDVIHQV
                     SVWTALAAANLKKGDEVGVYMEKGWLYAGPGVRSATGTAITDARANEDVGVARLSGII
                     EHREGTIHVCKVPRIERGGSRKVRRELLKEVAGRAQVVAAVGLEAWVALEAVGRRPDM
                     FFGAREGVIEAAFHGLDCAIVIVDEEFTDFLKRLEAAGLGYTIHDTVSP"
     gene            complement(18591..19106)
                     /locus_tag="VKO45_07405"
     CDS             complement(18591..19106)
                     /locus_tag="VKO45_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HMA05737.1"
                     /translation="MTGHIRIVNDPIEMVSLFVTFQNEQYKEIYEHLARAWMTEEELG
                     KLAGHDSVGECLAILKKGNLVEEKWRTPPPGKKPVKEYRTIYSRFRANFQCNMTDLGD
                     LIHVALSNDETVRNLVDQIEEEIGQGNTSINDIARKYNVSPVVIKGLAKRIPRLDVRG
                     QGMVLIDHGKR"
     gene            19164..21407
                     /gene="hypF"
                     /locus_tag="VKO45_07410"
     CDS             19164..21407
                     /gene="hypF"
                     /locus_tag="VKO45_07410"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HMA05738.1"
                     /translation="MRDRGRILVRGIVQGVGFRPFVYARAKALGIRGTVKNTGSQVEI
                     DAWGDRFDAFLSEVSRGPPLARIDSVEVLPLAGDPPASFEILASSSGSLSGMIPPDVA
                     TCGECVDDLFDEKSRYRGYFATSCVNCGPRYSIIESLPYDRERTSMASFPMCPECGRE
                     YTDPACRRHHAQTIACPACGPRLSLMDADGRPMEIPDPIREAADLLDAGSILAIRGIG
                     GFHIACTEERAGELKARLGRTEQPFAVMVREEALGRMAWVGDEDWKVLRSEVHPIAVL
                     VKRDPTAHTGISNLHTIGCMLPYAPLHHLLFSSLRAPLLIMTSANMPGYPMITEIDTA
                     VAKLRGQADYYLTHDRRILNRCDDSVVRDGFIIRLSRGIAPKRTRIDLGTSCILGVGP
                     ELSSNITIYKEGFAITSPHVGHVRNPPTLAYLEETAERIRSWLGAKYDVIAHDLHPSF
                     LSTGYARALAEETGAELVPVQHHRAHVAATTLEPCIGITIDGVGYGDDGRVWGGEVFA
                     GQVPHLDRVGHLEEVPMPGGDLATRFPERMLYGILPGERVMEILRGRGWTDIQCGVLA
                     RQVERGVNVAWTSSTGRVLDAVSALLGICRERTYDGEPAMRLEAAAHGGIASDWPLEF
                     LEKPGAEVLSTRSLVRRALDEYLAAGRSGGTEGKAVADIAASVQENLARGIAHIAIHA
                     AHDRKLGTVTLSGGVAYNAAIRETIRREVEAEGFSLFMNRDYPIGDGCISYGQCVWAG
                     LVKRETG"
     gene            21479..21823
                     /locus_tag="VKO45_07415"
     CDS             21479..21823
                     /locus_tag="VKO45_07415"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HMA05739.1"
                     /translation="MGSQDQIFAAGIWDVRPGNEQAFIEAWKEFSAWTALHQGGSGYG
                     NLLQDLDNPSRFISYGPWDNLESVQAWRRQPEFRKAIARFMELCDHITPGTFRIVAGD
                     APAEAGWKPGGY"
     gene            21909..23309
                     /locus_tag="VKO45_07420"
     CDS             21909..23309
                     /locus_tag="VKO45_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMA05740.1"
                     /translation="MEDRSVGRIALFIAILAGFLGPFDISAVNIALPTIGAEFSLDAL
                     ALSWVSTAYLLSSALFLVPFGRIADIVGRKRIFTLGLSLFTAASLAMALTVMPATLIP
                     LRVIQGMGGALIYGTAVAILTAVTPPEDRGKALGIYTTAVYVGLSIGPFLGGFMTGAF
                     GWRSIFLVNVPTGLFALWLVRTRLHGEWADARGESFDTPGSLLYGLSLVAVMLGFTWL
                     PGPPGIVMILLGVLLLAGFVRREQGTRHPLLDVGLFSGNRVFALSNLAALINYSATFA
                     VTFFLSFYLQSIRGFSPAAAGAILVTQPILQAVFSSPAGRLSDRVEPGKVASAGMGLC
                     ALGLLLLAFIREDTPLSFLLLALALLGTGFALFSSPNTNAVMSSVERRCYGVASGTLG
                     TMRYVGQMLSMGIAMTLFAVHIGRVEIGPANYPGLLAAIQAGFLIFTILCVIGIYPSL
                     ARGNMRDQEKAGCRVS"
     gene            complement(23468..23974)
                     /locus_tag="VKO45_07425"
     CDS             complement(23468..23974)
                     /locus_tag="VKO45_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05741.1"
                     /translation="MKRDAFLFVVSTVLITVACGCLIPATHGSQQVPSPIPTVAVPLT
                     IRISATPPRYNPAMSSTIGIRLTPVNTSGIITPDARFTWETTFGSFYRWGPPDFKVTE
                     LGARYTGTEEPVYWSYFSELGEKARRPVNITLTVWSQTDRLILANATLRIGWEDRSGF
                     TSVVEGEG"
     gene            complement(24463..24783)
                     /gene="rpl12p"
                     /locus_tag="VKO45_07430"
     CDS             complement(24463..24783)
                     /gene="rpl12p"
                     /locus_tag="VKO45_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HMA05742.1"
                     /translation="MEYIYAALLLHHAGKKIDETSVKSVLTAAGVAVDDSRVKALIAA
                     LEGVNITEAIAKAAVAPVAAAPAPAAAAAPAAAATAAAHEEKKEDKKQEEESAMAGLG
                     ALFG"
     gene            complement(24815..25669)
                     /locus_tag="VKO45_07435"
     CDS             complement(24815..25669)
                     /locus_tag="VKO45_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HMA05743.1"
                     /translation="MALYTHHLPQWKRDEVEKIKTWAKEYALLSLVDIHGIPAAQLQG
                     IRRDLHGKAVLKMTRNTLIEHAFAELGGDYADLEKFIAGQSALIFTDQNPFSLYKTLE
                     RTKTKMAAKPGEVAPQDIVVPRGPTNFKPGPIVGELQQAGIPAVIEAGKVRIRDTKVV
                     VKKGDVISKKVADVLSKLDIKPMDVGLRLQVAFYEKAVFRSEMLAIDEEAYRRDIILA
                     ARQAFNLSVNAAYPTKVTVAAIIGKAFSEAKNLAVEASVYEKDAMDAIIARASREAKT
                     LKGIVGGK"
     gene            complement(25671..26312)
                     /locus_tag="VKO45_07440"
     CDS             complement(25671..26312)
                     /locus_tag="VKO45_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HMA05744.1"
                     /translation="MVEKEKIAATVKQAIESAPKRKFRESVDITVNLKNIDLSQPKNR
                     IDETILLPHQLGGVKIAVLGKGDITSRAKDAKVDLIISSEEIERLGGAPREARKIAAE
                     YRFFIAETSVMSQVGRFLGPRLGPRGRMPTPVPPGTDIRPMVDRLRASVKIRTKDRTT
                     FHARVGSTEMDPNLIAENIDVVMKRIESVLEQGPLNIRSVYVKTTMGPAVRMV"
     gene            complement(26365..26838)
                     /locus_tag="VKO45_07445"
     CDS             complement(26365..26838)
                     /locus_tag="VKO45_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HMA05745.1"
                     /translation="MGEIVEVLVPGGKATPGQPLGPALGPLGINVKAVVDEINRRTAD
                     FNGMQVPVKIEVDDKKQFTVTVGTPPTTALIKKEAGIEKGSAQPSTQLVGNLPLEAAI
                     RIARMKAGDMLSYDTKKRVREVVGTCVSMGVTVEGRRPKEILAAIDAGEFDARLA"
     gene            complement(27146..27679)
                     /locus_tag="VKO45_07450"
     CDS             complement(27146..27679)
                     /locus_tag="VKO45_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HMA05746.1"
                     /translation="MTDEAAQPPQGPAPAPAEPASPATAGNRIFAIKTTAKQERAVAD
                     SIKKAVETQTDIKVVAIIVPDELRGYVLVETPESHARIEQLVEMIPHARAVVKGETKI
                     EEVAHFLVPKPVVSGIEEGTIVELIAGPFKGEKAVVKRVDMGKEEITVELYESVVPIP
                     ITVRGDHVRVVEKVEDQ"
     gene            complement(27690..27866)
                     /locus_tag="VKO45_07455"
     CDS             complement(27690..27866)
                     /locus_tag="VKO45_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HMA05747.1"
                     /translation="MAFKLEEELFHKYWRVIKLARTPTREEFQKIAIVAAAGIILIGL
                     IGFLILSFMGVLPH"
     gene            complement(27874..29007)
                     /gene="ftsZ"
                     /locus_tag="VKO45_07460"
     CDS             complement(27874..29007)
                     /gene="ftsZ"
                     /locus_tag="VKO45_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMA05748.1"
                     /translation="MKSIIDEALARGEEEQKATAAGAQPPVVPIAAPMPDKELEEVLS
                     QLKTEIAVIGCGGSGSNTISRMMEEGIHGARLVAMNTDAQHLIRTQAQYRILLGRQRT
                     RGLGAGSIPQVGEEAAVESVEEIKKIVAGCDMVFLTTGLGGGTGTGAAPVVAKAAREQ
                     GALTIAVVTLPFTAEGSIRRENAEAGLERLRDIADTVIVVPNDRLLEVVPRLPLYAAF
                     KVADEVLMRAVKGITELITLPGLVNLDFADVRTVMERGGVAMIGMGESDAEDKAADSV
                     KKALRSPLLDVDISGATAALVNVVGGPDMTIAEAEGVVQEVFDRVDPNARIIWGAMVD
                     PTMQHRMRTMLVVTGVHSPQIYGRAEKEAPRRVAKHFEIDFMK"
BASE COUNT         4886 a         9682 c         9428 g         5063 t
ORIGIN      
        1 ccggatacgt agacgacagg aaggactgcg atgataccaa cccggccatc aatccccttg
       61 gcaccgaggt ctgcgacggg aaggacaacg actgcgacgg gcaggtggac gaggggctcc
      121 ggcagacctt ttactgggac cgggaccggg acggtgtggg tggttcggac accatccagg
      181 catgcactca gccgccgggg gccgtgacct cgaccggcga ttgtaacgac aatgactggt
      241 gggtgtaccc gggtgccacc gagtactgcg acgggaaaga caacgactgc gacggtgccg
      301 tggacgaacc tgacggcctc gtctgccggt tcgccgacaa gggggacggc accgtcctgg
      361 acacggccac cggcctgtac tggctgaaga aggcggatct cggtggcggc acgatgaccc
      421 tggccgagac ccgcgccctg gtgaagaacg tggcggacgg gaacaacggc ctctccgacg
      481 ggtcatgggc cggtgcctgg aggctcccca ccatcgacga gatgagcggc ctgtgcgggt
      541 accggtgcgg gggccccggg gggctctgct ccctgggtaa tgcagccggg atcgggccgt
      601 ggcgcgaggg ggatgccttt tccagagtac cggtcaaccc ctccaacgtg tactggacgg
      661 acaccaccgc ggacagcggc aggcagcgcc tcttcgcggt gtatggcggg tactgccttg
      721 gcctgccctg gctctcagcc gaacccgact ggcccttcta ctactcctac cacgcgtggc
      781 cggtccggga ggctctcgtg gataacgacg gcgacggcta ccatgacctc gccggacccc
      841 gggtggggga ggactgcgac gacacgaacc cctccgtcaa ccccgggatg acggagagct
      901 gcaacgggat cgacgatgac tgcgatggga gggtggacga gacctgcgat tacgacttcg
      961 acgggttcac gcccgagcag ggggactgcg aagaccggaa cgcttcgatc aacccctccc
     1021 aggaggagga gatcaacggc atcgatgaca actgcgacgg ccatatcgac gagcggttca
     1081 ccgacaaggg tgacgggacg gtgctcgaca acctccggca catcaggtgg ctcaaggatc
     1141 cctcgggcat cccgccccgc acctggctgg aggccgtgaa caccgtcgcg gccctggggg
     1201 acggccagta cggcctctcc gacggctcga agccgggcga ctggaagctc cccacggtgg
     1261 ccgagttcaa caccctcgcg gacccgcgct tcgcctacga ccagtgggga gactgccagt
     1321 gcgaggtcct cgatcttccc aagtactaca ggaaatgctg gctcgaggtc cccacgggcc
     1381 cggccctgtc caactcgcag gggaatgccc actggagcga gggagatccc ttccttgacg
     1441 tcgacgagct gaacaccgcc tgcgatggtg actgccagag gctctgccag caccagtaca
     1501 taagttccgg cgggttcgtc atcgccatcc cctggggtga gtgcgtcggg cagacccagg
     1561 tgtgcaacct gaacctcttc tggacccggg agaccgtggg tgcatacctg ggggtcgatt
     1621 ggagcgaatt gaccgacccg aagtccaccc gggagtgcag gagctgcggg tcggtgtggg
     1681 ggaacgtgat ccagggctac tggggagact ggccatggag tgatccggga ggcacctgct
     1741 gtgtcaccta tgatatcaac cgggggacgc ccccagacta ctggcccggg tacgactgca
     1801 accagaagct gaaggtgtgg ccctggaggc cggcctgagc ccgaggcccg tggtgggcgg
     1861 ggagaggcgg tgagaacctg cggggtcccc cctttcctct caccacgcaa ggtccaggat
     1921 ggttgaggtt ggtcgccgga gaacgcggga gagagccatc gaacccggtt tcaaatccct
     1981 gcggatccgg cgtctgggtg gccggaggat ttccaccgga ggagaaggaa gccgctggag
     2041 ggattcgaac cctcgacctg cggtttacga aaccgccgct atgcctctaa gccacagcgg
     2101 caaccgacag atcaggtggt ttccgaaggg tattaaggtt gatccattca cggcggggtt
     2161 cttccaaatt ccgggatatc ccgcaatctt cttatcccat cggcgtacta cccctcaccc
     2221 atgcagcgac tcaccgcaag cgaacgtctc cctggaggga tcaccgtcta ctgggaagag
     2281 aagggggagg aggaggagac ctccttctcg ttcatggaac tcatcgaccg caggatcaac
     2341 tccctggatc tcctggaaca cccggccaag tacgtttttg acaatgacac ggggcacctg
     2401 atcctgaaac cctgaacttt ttcctttttt tcaactcctg aaaagatacg ctattgccaa
     2461 atagcttgat gacctctcct ttcgttatgc agaaaatcgt gagaaccgag cgatgcggag
     2521 acggggtcaa tgtggtctgg aaaaaggcgg ggaaggatac gacctcctac ttctccttca
     2581 cccagctcat cgacatgcag gtgaatgccc tcgaccttct cgcccacccg cagagctggg
     2641 gcatggatga gaagaagggg aaagttgtcc tcgtggtgta agggatcttc cctggactga
     2701 ggcgggagag ggtatgcagc cgatcttaag aaccgaacgc cgtggcgacg gggtgaacgt
     2761 ggtctgggag atggccggag aggagatgaa ctcgtacttc accttcgccg agctcatcga
     2821 cctgcgggtg aacgccctcg acctcctcaa caccccgtcg aactatggca tcgacgaaaa
     2881 aaccgggaag gtcggcttcg tcgccttctg tcatctgtca aggcgtcacg agtagccgcg
     2941 catcgtgacg ttttcgacgg gctgcttctc cttcggggtc tcgatcttcc cgaacttccc
     3001 ctcgtagtcg gccatgctcg tccccaggac ctcgaggaag ttcttggcat gccgggggct
     3061 gatggagacg atggccttgg cccgggcctg gttcgtcccc gggatctcgt ggaggaagat
     3121 gaaggtgaac tcgtcctcct tgtacgcgat ctggatgcgg ttgctgtaca cggggtccag
     3181 gtcctggggg atctggacct ggatctccct cggcggattc atgagatctc cctccgggtc
     3241 aggaggctct cctccttctc cctcggaaga tggaaatctt ccttcgggtt cataaggaaa
     3301 aattttactc ctcccgcaat aaggctttgt gggagccctt cctgccgacg ggaaacgtcc
     3361 ggcggcggag agtagagatg ccgagaaccc tcgtttctgt ctccgagatc gtccgctgcc
     3421 acttctgccc ggtgcggttc tccctcgaac ggaaactcga ggcccgggaa tccccccggt
     3481 acaccatcgc gaagcaggtc tcctcccacc tcggcaggga tctcgacgag gatgggatct
     3541 gggaggaggt ggaggccatc ctcccggaga tcgaccccgc ctaccggtcg tacctctctg
     3601 cctcggtgga acgatgcagg gccggggcat ggaggatccc ctccgagacc gaggttcctg
     3661 tccggtccga ccggttcggg atccgggggc tcgtggacaa gatcttcgac tcggagccta
     3721 cctttgccat cacccggccg accgagtccc cggcgatcgg cgtttccacc tccgaccgga
     3781 tccgggtggc ggcctatgcc ctctgcatcc gggagagcct cggcatcccg gcgtcccggg
     3841 gcgcggtgga gtacatccct tccggggagg tcaggatctg cacgccccag ccacgggacc
     3901 ggagggccgt gatccgggcc gcggaggcgg tcaggtacgt ccgggagggg cacatcccga
     3961 ggagacctct ctctgcaccc tgtggcggat gtccccaccg ggagcggtgc gggaccggcg
     4021 cgaaaaaact ctccgacctc ctcgggtagt cctggaacgc taccccgatc cctcgaggag
     4081 ggtcttcgag acgatgtact cccccttcgc caccctcgtg gtgcccacca cgagccaccg
     4141 ctcggccccg tgcccttcga ggaccccgct cgcctccacc gtctccccgg gaagggcctg
     4201 gccgctgtag gtgtgggtga aggagacgat ccggttcacc acctcatgat ccacgaggta
     4261 gacagccggg ctgtcgaagg aaagggaggc gtcggtcacc cgggcctcga tggtcatcct
     4321 ccggatcctc tcgccctccg ggaccggccg gccgtccagg tcatcgtagg cgcgggtgaa
     4381 gaggaggtcg aagtaggtcc ctccgataac cccccggttc cacttcctct tttcgtgaag
     4441 gacgaaggac tcgaagggga tctccggttc ccgctttgta tacaccgtgc gccacatctc
     4501 ctccgagagg ccggcaaggg acccccgttc cgtttcccgc ctgagcgttt cctgggccac
     4561 aggaaaggca ctcccgtagg ctacgaggtc gatgtcggag gccggcgact ccatcccgag
     4621 gaggcgggag ccggtgcacc cgagggctcc ctccgggagg gcgaagagat ccacgagcgc
     4681 ccgaacccgc cggtcccggc cggcgcagaa atccagcctc tcctcgggct tgtagacgcg
     4741 tctcacgtcc ttcccgggga tacggaggaa ccggcccgtg taggagggct tcttctgcct
     4801 cacgagatcg aaggcctcct caaatcccgc cttccggtac cgcacaccct cgggattcac
     4861 ccggtcgccg gatgggtcgg gaacgtagcg gaggatgcag cccacccggt cggcgttgtc
     4921 ataggtcgcc accgagtaga tccagccgtc ccggtcctcg atgaagtcgc ggaggcggag
     4981 cggcttcatg ccgcgcctcg cccggatccc ctcacgcgcc cctccatcgg gtcccctcac
     5041 gcaccctgcc cccggagata ttcctccacc tcctctgccc tcgtccctct gcggaggatc
     5101 tgcttctcga ccaggtcaac gacggtgctc ggggtaccgg ggagaaaccc cccgtcaacg
     5161 aggaggtcgt acgggacccg gacctccgag gggctcaccg ggtccttctc gcccgagacg
     5221 ttcgcggagg tcgcggtgat gggggagtca aagccccgga tgatggcgag ggccaggtca
     5281 tgggccggga tgcggacccc gatcctcccc gttcccccgg tgagcatatc cggaatgcag
     5341 gaccgggccc cgaggatcac cgtgaaggga ccgggaagga accggtcgat gaactttagg
     5401 tcgtattcgt cggcgcaggc gatggcgcag agcatctcca cgtccgatac ggcgaccgag
     5461 accgggttcc cgaggggccg gtgcttggcc tcgtacaccc ggagaatggc accgtcggag
     5521 aaggcgtccc cgccgaggcc gtagaccgtg tccgtggggt agacgacgat cccgtcccgt
     5581 ctgaggacct ggatggccct ctccacgatg tccatcccta gaactcctcg ccccgcaccc
     5641 ggccgatgtc gaaactcgcg taccgggaga cgtgcatcac ggcaaagacc ccggcctgga
     5701 tgggatcggt caccacgaga tcggcgtgct tcgggaccga ccccgccatc ttgagggcga
     5761 tgaccgggat gcccgcctcc cgcacccggt ccaccgcctc ggagatcttc ccgcccatga
     5821 gggagcccgc gaggacgagg atctgggccc tcgggagccg gaccaccgcg tccacggcct
     5881 gggcgaggtc ctcctcaccg acgaggggga tggtgtccac cgagatccgc tcgccccgga
     5941 tgttgtgccg gtcggcctcg ttcaccgccc ccagggccac ctgggccacc tgggcacctc
     6001 cgccgatgat gatgacccgg gagccgaaga tccgggagaa ggaggcatag gtgacgacgt
     6061 ccctgaccga cgggaggttc aggagatcgg ccatgagctt cccccggtcg tccccgccct
     6121 cgagctcgaa gtagagggag gcacagccgg tggccggtcc ggacgggatg agggactgct
     6181 ggacgatgac gatattcgcg tcgtgggccg cgagaacccc cgcgatgtcc cggaggaccc
     6241 cgggccggtg ctcgcaggtg atgctgatgc cgtagatctc aggcgtctcc ataggtcccg
     6301 gatgcgatag ccgggattcg aacccgggct aaaggcttgg gaagcctctg tcctgccgct
     6361 agactactat cgcgtctgcc tgtagagtat cgggattcct gcatatagat tctttctccc
     6421 tcacgccgtg cagcccttcg ggagggtgaa ccggatggcc gcccccttcc cgggttcccc
     6481 ctcgacccgg tccgccaccc agatccggcc cccgtagccc tcgatgatca tccggctgat
     6541 gtagagcccg aggcctttcc cgctcctcga gctcctcccc cgctggaacc ggttgaagat
     6601 ggcgggcttc agatcgtcgg ggatgccggg accgctgtcc tcgacggaga cggtcacccg
     6661 gtccccgtcc tccgctaccc gtatccacac ctgggcgccc gtacccccga acttcgcgct
     6721 gttgccgatg aggttccgga agacctcgga gagaaggctg ttggcacaga ccgatacagt
     6781 ggtcccgtcg tagtggatgg tgacctcggg atggtgatcg agctccatcc tgatgaccgg
     6841 gtccaggttc accgcccggg tcgcctggga cctttcctgg atcgcccgga tggtgctcac
     6901 gttccggatg atctccacgc tctgctggac ggcgctctcc agcttggcga gcatctcccg
     6961 gctcttcccg gtgaccaggt ccgccaggag ctgggcgtat ccgaggctca cggtgttcac
     7021 gttgttgata tcatgggaca tgatgtccag gtacaggttt gccttcgtat tggcaaggcc
     7081 gagctcctcc tggagcaggg tctttttcac cgcactcccg atggcccggg agaccgattc
     7141 gagcatcgcc atctcgtcct cggcgagggg cgccgccttt ccccggcccg ccgcgatcag
     7201 gccgagggcc tgggaccccg cggggagcgg gatgagcacg aaggagagga cccctggcag
     7261 gttcaggtcc ccgaaagaat tcttttcccc cgtgagcacg ggcaggcccc ccgcgaggat
     7321 cccccggaag ggctccaccg agaggtcgat ctccctggat cttccggcgg cctcgtccga
     7381 cagcccctcg tggtatcgga gggatccctg cctcccgtca tccgacacca ggtagaccat
     7441 cacggcggcg gacccgagca tctcccggat cctccttccc gccgtcgaga ggatctcgtc
     7501 cagggacgcc gaggtcgagg cggccgcgat gatctcgttc atggccgaga ggtgccggtt
     7561 ccggcgcagc aggttcccgg cgaggtacga gatgatggcc gccacccaga tgaagacgag
     7621 caaccggacg aaggcccgga acagcgggtc cacggcatac ccgctgagga agtacacgag
     7681 gccggcatag atcgccccga ggaaggccgc aaacccgatg gccttcgacg ggttccggta
     7741 ggcgttcagg aggatgggga tgtagtagag gtgggggaac acatcggtga tctccctcgt
     7801 taccgagacg atggtgaccg caatggcgag gagggaggtg accggtatcg cgaccgccca
     7861 gaggacgcgg gtgatgtcct cgtccgagta tttccccagc atggagggcc ccttctctcc
     7921 aggtaccttg gaacgatgag tccataatgg ctacccctcc cgggagaagt cgcacccctc
     7981 ctgatggcgg gtcgctccat gtcggtttct cgcgacttcg tcgctcgagt tcgggttctg
     8041 atgaaccgtc cctcttacga aacctccatc gataccctta tcacggcgcc cggtccacgt
     8101 attcctgcac cccgagggtg ccggcgattg taagtccgac gtgccgtcaa aggcatggcc
     8161 cgaaaaacgg gctccgggat taccataggg ccgttccagg ctcttttccg ggcgtattac
     8221 agtcccctgt cacaggaggt caccatggcg agaatgcatg cccgccggag gggaaaggca
     8281 ggttcggtcc gtccgtaccg gaaggaaccc ccggcctggt ccaacacgga cgtaaaggag
     8341 atcgagaagg tcgtgagaga ggagcgccgg caggggagat cggccgccga gatcgggctc
     8401 ctcctgcggg acaagttcgg ggtccccgac gtgaagctcg cgaccgggaa gcgcctcggc
     8461 cagatgctcc gggagatggg gatggagtcg aagatccccg atgacctccg gaacctcatc
     8521 gagaaggccc tgggcctccg gaagcacctc tccacgaaca agaacgacct gcacaacaag
     8581 cggcagctcc agctcatcga gtccaaggtg cgccgcctgg tgaagtacta catccggtca
     8641 gggaccctcc cgcaggactg ggtgtacaag ccggagaccg ccgagatcct cctctcccga
     8701 tgagaccatg gcactccacg cagacgcgga acagctcgcc ggccacctcc tcaagcagga
     8761 gtttgtggag atccacgcac accatgacgc cgacgggatc gcggccgggg ccatcatcgc
     8821 aaccgcgatg caccgctccg gccggtcctt caggctgcgg tgcagccccc ggatctcccc
     8881 ggccgacctc gcgggcgagg gaggcgtgct cctctgcgac ttcggggcat cgaggaatga
     8941 tctccctccc catgtcatgg tcatcgatca ccacccaccc cggttcggcg gggagttcca
     9001 cgtgaacccg gccaggtccg gggatgggac ggggggcgag gtcgcggcct ccggtgccgc
     9061 gtacctggtc gcccagcaca tgggcgagaa ccgggacctc gccggcctcg ccctcatcgg
     9121 catggtcggc gaccggcagg actgcaccgg ggcgaaccgg gagatcctga acgacgggat
     9181 cgcgaacggc ttcatcaccc cgggaagggg attctccctc gccgggaggg atcccgtcga
     9241 gtctctcagc ctctcgaccc ggccttacct ggacgggatc agcggcgacg aggcggcggc
     9301 gcagaggctc gtcgcggagt gcgagaggga cgggaagctc tccctcgatg ccctcctctc
     9361 cctcgtgatc ctcaggtcgg ggtcctccct gtccgccccg gccctcgagt ccctctacgg
     9421 ggacacctac accctcgagc gggaggcggt ccccgacctc cactccctcg cggccctcgt
     9481 ggatgcctgc ggccgggcgg ggatgggcgg cctcgcggcc tccctcctca tctcccggcg
     9541 gccggccgga accgatgcct gggaggtgct ccggtcctac cgggcccggg tcatcgccgg
     9601 gatccgctcg gcgcagcccc tggagacgga cgggtggtac ggcatcggcg acgcctcggt
     9661 gaacggcccg gtggccgacg ccctcgtcct ctcgagggag gggaagggac cggtcttcgt
     9721 cttcgcgccc ggcgacggcg tctggcacgt ctcggcgagg tgtccgccgg gggtgcggat
     9781 ggacctcgag aagatcgtcc gggacctcgc cctggcctcc ggcggcgacg ggggaggcca
     9841 ccggtgccgg gcgggggcca ccatcggcgc cgaccacctg gaccggttcc ggaagggcct
     9901 cgcggaggcg gtggcctcat gatctccgtc agggggaccg tcaccacctc ccaccgcgac
     9961 cccgcacggg tcgccggttc gctctcgccg gacaacctct cctccatgag catgagcccc
    10021 ggggagggca tcgtcgtcat ggagatctcg ggcgggaacc tccggtccat catcgcatcg
    10081 gtcgacgact acctcatgaa cctcgccatc gcggaggacg tatgcacgtg cacttctccc
    10141 tgaaggccga catcatcttc tcaggggata tcggcaccga ggcacgggcc tccgtggacc
    10201 gtgccgtgac ggaggcgaac gaaaaggtct tcccgaaggg tgtgccgaag ggcgtcccgc
    10261 cggaggaggt ggggaggatc acccgatggg aggttgaggg gaaccgcctg gcgctcgaga
    10321 tcgagagcgg cgggtatacc cgggcccacg acgccctctt ccggttcagg aagctcatcg
    10381 gggccgccct cgggaagcac cagctgggac tccgggagat cggggtcagg gagttcatgg
    10441 cgggcatcac cggcgatatc ccggagacgg cggtcccccg gctccccttc atccgcggcg
    10501 tgagccggga ggccggctgc gtgttcctcg acctcgcggt caccacgcag gacctggaga
    10561 accggatacc ggaccggatc gtgaggctta tcgaggagaa ggtagaggcc gcgggctacg
    10621 gcgggaagac cgagcactgg gagctcatgt acgagagccc gaagaaaccc cgggtctaca
    10681 ccggaaaccc caccgaggag atggagaagc gccactggat ccggcacggg tcctcccggg
    10741 gccagtggat ctacgccccc cagcccatcc agctcttccg ggccttcacg tccctcgtgg
    10801 aggaggagat catcaggccc ctggggttct cggagatggt ctttcccaag ctggacacct
    10861 gggaggtcct ctcccggtcg gggcacgcga agggggtcta cccggagatc tactatgtct
    10921 gcacgccgaa gacccgggac ccggccttct gggaggatgt cgcggactac ttcaaggtca
    10981 ccggggtggt ccccgtcgag atgctccggg agcggatcga cgggcccatc ggggccctct
    11041 gctatgccca gtgcccctcc ttctggccct ttgtccaggg gagaaccgtg gccacctcgt
    11101 cgctccccgt ccggatcttc gaccggagcg gcacctccca ccggtacgag agcgggggga
    11161 tccacggcat cgagcgggtg gacgagttcc accggatcga gctcgtctgg ttcggcaccg
    11221 tcgaacagac gacaaagatg gcagaggagc tccaccgggc gtacacccgg gtcttcaacg
    11281 agctcctgga gctcgagtgg cgggccgcga aggtcacgcc ctggttcatg gcccaggagg
    11341 gaatggtggg ggcctgcggc tgtgagtccg tcgggaccac cgactacgag gcgttcctcc
    11401 cgtacagcgg gagctggctc gagttccaga acgtctcggt gaacggggac aagtacccga
    11461 agggcttcaa cgtgaagatc cagaacgggg aggagtgctg gtcaggctgc tcgggcatcg
    11521 gcctcgagcg gtggacggcc gccttcctcg cccagaaggg cttcgacccg gcggagtggc
    11581 cgggcgcggt cagggagcga gtgggaacag tgaacgaggt tatgagattc ctgtaaggag
    11641 acgaaacata tggcaaagaa aagtcagatc ggaaggaagg tcgagggctg gaaggcgaag
    11701 gcctggtaca gaatccatgt gcccgaggtt tttggcaaga gctacatcgg ggacaccatc
    11761 tcgagcgatc cggagaccgt gatgggccgg atcctccaga caaccctcgg cgagatcatc
    11821 caggactaca cgaagcaaca catcaagatc cgggtgaggg tgacctcggt ggccggcgac
    11881 gccgcctaca cggagttcgt gggccacgag ctgacccggg actacatgcg ctccatggtg
    11941 aagcggcgca cctcgcgcat cgacaccgtt ctccccgtga cgacgaagga cgggaaggtc
    12001 ctgcgcatca ccatgacctg cctcaccctc acccgggcga acctctcgca ggtgcatgcc
    12061 atccggtcgg cgatgacccg gttcatccag tcgtacgccg cccagcggga gctccccgcc
    12121 ttcgtgaagg acatggtctc gggggagatc acgaaggagc tcttcaagct cgcgaaaccc
    12181 atctaccccc tgcgccgggt cgagatcatc aagtcccggg ttgagccgga ggcaaaggcc
    12241 ggggaggccc cgccggccca gcccccggag ctcaccgagg aaaagccggc ccccgaaaca
    12301 aagccagagg agaaggccga ggtcccggcc ccggcgcccg cggcctgata tcccttacac
    12361 aggaatccgg ggattttccc cccccgttcc cccacgagcc ttttttcggg tgttcccgca
    12421 tcactacaca aggtcttctc tatcccgaac cccattttct ccccggggga ttcggtcggg
    12481 cggcgggggt gggtggccgt gtcggtgagg gcaaggggac atccttctct ggccggttct
    12541 catcctcccg cgaaggaaat gggtgggtgg aggggggtcg gaaggcggga gcggaccctg
    12601 cagggtaatg aaggaggagg agaaacactc tgtcatgact gcggggagag ttctcctcct
    12661 tgtcctggac ggcatctccg accggccctg cccggagctc ggcggcagga cgccgcttga
    12721 ggcggcggag accccggtcc tggaccggct ggcccgggag ggcatctgcg gcatcatgga
    12781 ccccatcggg ccgggcatcc ggccgggctc ggacacgtct cacctctccc ttctcggcta
    12841 cccgcccgaa cggtactaca ccgggcgcgg gcccctcgag tgcgaagggt gcgggatcca
    12901 tatggagccc gggatgatcg gctttcgcgc caactttgcg accctggaca aacaaggcaa
    12961 ggtctcggat cggagggccg gccggatcca cggaacggat gccctgtgcg acgccatcga
    13021 gaaggaagtt cacctgaagc acgggatcag cttccggctg cggcccggcg ccggacaccg
    13081 ggcggccctc gctctcaggg ggaaggatct cggggcctgt gtctcctcta acgaccccaa
    13141 gaccgaggga atcgcccctc ccgaggtaaa acccctgaca acggaggcga gggaccggag
    13201 gaccgcagag gcatgcaacg atttcctcgg acaggcgcat gacatcctcg cccgtcatcc
    13261 cctgaaccgg aaccgggaaa aggaggggct cccccaggcg aacgccatcc tcatccgggg
    13321 ggcgggggag atgggccggt tcgagccctt cgccgagcga caccggatga ccgggagcgt
    13381 catcgcggcg gcgaccctca tcaccgggat cgggaaggcc gtcggcctgg aaagcatccc
    13441 ggtccccggg atcaccgggt cggcggacac gaacctcgac gggaaggtcg cggccgccct
    13501 cgcggagctc cggaggaagg agttcgtgct cctgaacatc aagggggccg acgaggcggg
    13561 ccacgacggc caccccctcg agaagcggga cttcatcgcc cgggtggatc gggccctcac
    13621 tccccttgta tccctcgacg atactctcgt cgtcgcctgc ggggaccaca gcaccccctg
    13681 caccgtgatg gaccacagtg cggaccccgt gcccctcgtg atccggggcg agggagtccg
    13741 ggtggaccgg gtcgcccggt tcaccgagac ggcctgcgcc gaggggggcc tccaccggat
    13801 cccgggatcg gcgctcatgc ccatcctcct cgacctggtg aacagggcaa ggaagtacgg
    13861 ggcctgatga aggccgccgg catcctcgac ctcggggaga tcaccctcct cgaacccacc
    13921 ttctcctccc tcaccgagga tctgtacacg ggagccctcc tcgggatccc cgcgaccggg
    13981 gcgaggatcc tcatcgacac cggggagaac cccctcgcgg tggccgtcac cgacggggag
    14041 ggatggttcg ccgcgaactt cctcttccgg gagccggccc gggaggtcat cgaactctgc
    14101 gagaggctcg atgccgaggt ccaccaggcg gagcgatccc tctggctcgg agccgtccgg
    14161 gagtactaca gcctcctcct tgccggatcg gtcaccccgg ccctcgacga tctcccgccc
    14221 gatcgcctgc ccgggctcac cgatctcctg agggagatct gggggaggga ggaggacatc
    14281 ccgtgcctgg actgctgctg cgggtcgggg gccggatccg cggccgcccg ggccctcggg
    14341 atgagtcctc tttcgtacga caacgatccc gccctcctct ccctcggact ctccacgggc
    14401 cggcttctcc cccctgagac catgtggatc gatgctaccc aggccggccg gtacatcggg
    14461 ccggttcccc gggggatcgc ggtcatgatg ggggagatca acgccttcac ccgcgagatg
    14521 tgggagagca tcaccgggga gttcctcggc ctcgtggagg aggcgctcat caccgtcggg
    14581 acggaggcgg aggcccggac ggtagagacc tggtgccggg cggccggaca caagcccatt
    14641 gtctcggaaa acccgagaca cgccttttat gaccggtggg tcgtgcggtc agggaagatg
    14701 caataaataa tggcgggaaa agaggggaga gggcccatca cgggcggggg atgcgcttca
    14761 gcctgtcgag aacgaccatc gggttgggga gcttgtgggc catcctctcg aggaacttcc
    14821 ggacgaagag gtcgcttgag ccctgcttct cgatgatttt ctggatatcg atccccaccg
    14881 gtgtgatggt ctctgaaatg tacttgagcc ttttatcgag cgctgcatcc agatacatgg
    14941 tgttgaccgt taccataaac cctgatcaac atatgccgtt tcttctataa ataatctttc
    15001 gcattttcgc ctgaaggcgg ccttttattc cctgaattct tccccgtttt cattcggttc
    15061 cccgggtatt ttcccgaata caatgaataa cacagaatcg ccgttgaact tcgatatcac
    15121 cccctgagtg ccccgatatc ctccatcccc cgaagccccg gggcccggca tcacagccgg
    15181 gagacggacg accgtctccc ggcgtaggaa acgaggaggg ccgccgcccc ggccacgatg
    15241 agaagggctc ctccggcggc gaggatcgcc cgggccacct cgcccgggaa gacggcgagg
    15301 acaatcccgc cggcaaggag ggcgaggccc acccagagga acgccctcct cccccagccc
    15361 cgggaggaga ggaggccccc gatgaagagg aggaaggccc cgaggaggac aaagatgccg
    15421 gcggcgaggt agatgatcag gtcggccacg agatccctgc ggaggaaggc gaggatgccg
    15481 acgaggatgg aggcgagccc ggcgatgaag aggagaacgg gggggatgcc cgccctcccc
    15541 aggaaatggc ccgcggcgag aaagccgagc cccaggagga tggcgaggac gcccaggaac
    15601 aggataacca ggctcacgat gacatcgctc cagaggagga ggcagaggcc cgccaccatg
    15661 aggaggaccc ccacggccgg aaggagggcg ggggggaggg tgatccaggt ctccgcgagc
    15721 atctcctccg gcatgggtac cggatttcgc caagccctac ataaactgga tcttttctcg
    15781 cccggcggtg cccgtcagcg ctcttcaccg gtgcactcga gttcggcgag gatctcgacc
    15841 gggtcctcca tttcaccgac gagcctccgg atctcgagga gtcgctccac cccgatgaag
    15901 tgctcggcca tcacccgccc gaggggcgag aggacgaggg attttccccg cctctgcacg
    15961 agcccgcggg agaggagctc ggggaggacc ggctccgcct cgccgaccat catccgcgag
    16021 atcctcccga tggcctcctc gtcgccccgg gcgaccaccg cgtgggccgc gaactcctcg
    16081 gagctctcct ccatgccgta ggagggcgcg acctcctcca tctcgcccct gaggagccgg
    16141 atggccacct cctcctcggt cgcctccgcc tccttcatgt agaccgctcc cggttcggcg
    16201 aggatgacca ccttcccccg gtcatggaag tccggcctcc ccgccctccc gcccatctgg
    16261 gagaactcct ggacggagag ccactcgatc cccatggcga gggagtcgaa gatcacctgg
    16321 gaggcgggga agtccacccc ggcgccgagg gcggcggtgg tcaccacggc ggcgatcttc
    16381 ccctcggcga acctcctctc ggtgtcccgc cgctcctggg cggtgagccc tgcatggtag
    16441 ggggctgcct tcctcccgag agcctcggcg atgacatggc acctcgcccg ggagttggtg
    16501 aagatgatgg tctggccccg gaaccccttc gaggaccgga ccgagaactc ggcgtccacg
    16561 agctgcttct cgaaccggat cttgtccttc ctcgtgacga agaggaggtg gcgttcgagg
    16621 ggcacgggcc gctcggcgta ggtgacgagc cgggcgccga gcttttcggc gaggaggtgg
    16681 gggagcccga cggtcgccga gaggtagaga aactgggccc cgggggcgag gaacttgagc
    16741 cgggcgatga gaccgtccag ccggtgcccc cggtcccggt cctcgagcat ctggatctca
    16801 tccacgacga cggtcccgat atgctggagg ccccgtccgc acctgaccag gtggtcgagc
    16861 ccctcgtagg tcccgaccac gacctgtgcg tccgggtcct gcctccccct cgtccggttc
    16921 tcggggaggt tcagccggtt cctcccggtg aggagggaga ccccggcgat cttcccgtac
    16981 cgctcggtga accgctcgta cttctggttc gcgagggcca ccagggggac gaggaagagc
    17041 atcctccccc tcccctcgag gaggttcttc atccccgcca tctcgccgat gaaggtcttc
    17101 cccgaggccg tcgccgagac caccaggagg tccgatccgg cgaggagccc cgccccgacg
    17161 gcgagctgct ggaccggcat gaggcgctcc acgccggagg cggccctgaa ctcgggaggg
    17221 aggggaagct cggagaccgc cgaggtctcg gtgacgggat gggcatcgat ccggtcgaaa
    17281 agggtgcggg tgaggtccag gccgtcgggc tgcagggccg agaggacccg gtccaggtcc
    17341 cggtatgcgg cgaggaggct ctcgatgtag gcgtacgaga ccttcccgaa ccgggtgaag
    17401 tacccgagct cccgcctcac ctccttcttc gcgcagtcca tgcagacgga ctccttcccg
    17461 taccggaccg aggtggcggg ctcgaggggg gtgacccggt cctcgaggag gcaggtccgg
    17521 cagagggtcc ggacggacca ggggatctgg aggtcggaga ggaagtcctc ccacggctcg
    17581 tccctcctcg tgagctgcac ctcggcccgc cggagggtcg cgaccaggtc cttcgacggg
    17641 atggaccggc cgtgcctgct cccgggcttc ctcacgaggt agtccttggg ccggtacccc
    17701 ttcggggtgc ccacgaggtc cacctgcccg accgcggtca ccccgccccc gtcgaggaaa
    17761 aagagccggt agtacccccg ctggggatgg accacgatcc tcatggggag accgtgtcgt
    17821 ggatggtgta cccgaggccg gcggcctcga gcctctttaa gaaatcggtg aactcctcgt
    17881 cgacgatgac gatggcgcag tccaggccgt ggaaggccgc ctcgatgacc ccctcccggg
    17941 ccccgaagaa catgtccggc cgccggccga cggcctcgag ggcgacccag gcctcgagtc
    18001 ccaccgcggc gaccacctgt gccctccccg cgacctcttt caggagctcc cgccgcacct
    18061 tccgggaccc gccccgctcg atccggggca ccttgcagac atggatggtc ccctcgcggt
    18121 gctcgatgat cccggagagc cgtgccaccc ccacgtcctc gttggcccgg gcatcggtga
    18181 tggcggtccc cgtagcgctc cgtaccccag gacccgcgta gagccacccc ttctccatgt
    18241 agacccccac ctcgtcaccc ttcttcaggt tcgcggcggc aagggcggtc cagaccgaga
    18301 cctgatggat gacatcacgc cggatgtgcc gggcataggc ctcgagcacc tcggcgtggt
    18361 ttaccaccca ctcgatgccc tcccgggtca cctcgtagcg ccccctcccc tgggcgctca
    18421 ccatcccctc ctccacgagc tcccggatgt actccgagac cgcctggggc gtgaccccga
    18481 gcttcccggc gatctcctgc tgccggatgg ccggctggtt ctcggctatc tcgacgagga
    18541 tctggaagcg ggtggcctcg ctcttggacc ggagaatggt ggcgaggggg tcatcgcttc
    18601 ccatggtcga tgagcaccat gccctggccc ctcacgtcca gccgggggat ccgctttgcg
    18661 aggcccttga tgaccaccgg ggaaacgttg tacttccggg cgatgtcgtt gatcgaggtg
    18721 tttccctgcc cgatctcctc ctcgatctgg tcgacgaggt tcctcaccgt ctcgtcgttc
    18781 gagagggcca cgtggatcag gtccccgagg tcggtcatat tgcactggaa attggcccgg
    18841 aaccggctgt agatggtgcg gtactccttc acgggcttct tcccgggcgg gggggtccgc
    18901 cacttctcct cgacgaggtt gcccttcttc aggatggcga ggcactcccc cacggagtcg
    18961 tggcctgcga gcttcccgag ctcctcctcg gtcatccagg cccgggccag gtgctcgtag
    19021 atctccttgt actgctcgtt ctggaaagtg acgaagaggg agaccatctc gatgggatcg
    19081 ttcacgatcc tgatatggcc ggtcaaagcg cacaacccat ttatatatgg tcccggagct
    19141 caataaaggt ttcagaatca cccatgcgcg accgggggag gatccttgtc aggggaatcg
    19201 tccagggcgt gggattccgc cccttcgtgt acgcccgggc caaggccctc gggatccggg
    19261 ggaccgtgaa gaacacgggc agccaggtgg agatcgatgc atggggagac cggttcgatg
    19321 cctttctctc cgaggtctca cgggggccgc ctctcgcccg gatcgattcg gtggaggtgc
    19381 tccccctggc gggagatccg cccgcgagct tcgagattct cgcctcctca tcaggcagtc
    19441 tctccggcat gatccccccc gatgtcgcca cctgcgggga atgtgtcgac gacctcttcg
    19501 atgagaagag ccggtaccgg ggctactttg ccacgtcgtg cgtgaactgc ggtccccggt
    19561 acagcatcat cgaatccctc ccctatgacc gggagcgtac ctccatggcc tccttcccca
    19621 tgtgccccga atgcgggagg gagtacaccg atcccgcctg ccggagacat cacgcccaga
    19681 ccatcgcctg cccggcctgc ggaccccggc tcagcctcat ggatgccgac gggaggccca
    19741 tggagatccc cgacccgatc cgggaggcgg cggaccttct cgatgcgggc tccattctcg
    19801 ccatccgggg aatcgggggc ttccacatcg cctgcaccga ggagcgggcc ggggagctga
    19861 aggcccggct cgggaggacc gagcagccct tcgccgtcat ggtgagggag gaggccctcg
    19921 gccggatggc ctgggtcggc gacgaggact ggaaggtcct ccggagcgag gtccacccca
    19981 tcgcggtcct cgtcaagcgc gacccgactg cccataccgg gatctcgaac ctccacacca
    20041 tcggatgcat gctcccctat gctcccctcc accacctcct cttctcgagc ctcagggccc
    20101 ccctcctcat catgacctcg gcgaacatgc ccggctatcc catgatcacc gagatcgata
    20161 ccgcggtcgc gaaactcagg ggccaggcgg actactacct cacccacgat cgccggatcc
    20221 tgaaccggtg cgacgactcg gtagtgaggg acggcttcat tatccgcctc tcccggggga
    20281 tcgccccgaa gcggacccgg atcgacctcg gcacgtcctg catcctcggc gtgggaccgg
    20341 agctctccag caacatcacc atctacaagg aagggtttgc catcacctcc ccccatgtcg
    20401 gccatgtccg gaatcccccg actctggcgt atctcgagga gaccgccgag cggatccgga
    20461 gttggctcgg tgcaaaatac gacgtgatcg cccatgatct ccatccttcg ttcctttcca
    20521 cgggttatgc gagggccctc gccgaggaga cgggggcgga gctcgtcccg gtccagcacc
    20581 accgcgccca cgtggcggcg accaccctcg aaccctgtat cgggatcacc atcgacggcg
    20641 tgggctacgg ggatgacggg agggtctggg gcggcgaggt cttcgccgga caggtgcctc
    20701 acctggaccg ggtcggtcac ctggaggagg ttcccatgcc gggcggagac ctggccaccc
    20761 ggttcccgga gcggatgctc tacgggatcc ttcccgggga gagggtgatg gagatcctga
    20821 gaggccgggg ctggaccgat atccagtgcg gggttcttgc ccggcaggtg gaacggggag
    20881 tgaacgtggc ctggacctcg agcacgggaa gggtccttga cgccgtatcg gccctcctcg
    20941 ggatctgccg ggagaggacc tacgacggtg agccggccat gcgcctcgag gccgccgcgc
    21001 acggggggat cgcctccgac tggcccctcg agttcctcga aaaaccgggg gctgaggtcc
    21061 tctccacccg ttccctggtc cggagggccc tcgacgaata cctggcggca ggacgttccg
    21121 gcgggaccga ggggaaggcg gtggccgaca tcgccgcctc ggtccaggag aaccttgccc
    21181 ggggcatcgc ccacatcgcc atccacgcgg cccatgaccg gaaactgggg accgtgaccc
    21241 tctccggcgg ggtggcctac aacgcggcca tccgggaaac catccgcagg gaggtggagg
    21301 ccgaaggatt ctcccttttc atgaaccggg attaccccat cggagacggc tgcatctcgt
    21361 acgggcagtg cgtctgggcc gggttggtga agcgggagac cgggtagccg cattcctccc
    21421 ccgcataggg agcatttttt actccggccc caccacacat catcaggggg catatcgtat
    21481 gggatcgcag gatcagatct ttgcggccgg catctgggat gtccggccgg ggaacgagca
    21541 ggccttcatc gaggcatgga aggaattctc cgcctggacg gccctgcacc agggaggatc
    21601 gggatacggg aacctcctcc aggacctgga taacccctcc cggttcatct cctacggacc
    21661 ctgggacaac ctggagagcg tccaggcctg gcgccggcag ccggagttca ggaaggccat
    21721 cgcccggttc atggaactct gcgaccacat cacgccgggg accttccgga tcgtggccgg
    21781 cgatgccccg gccgaggcag gctggaaacc cgggggatac tgacacgacc gggtgaagac
    21841 acgatacaag tcacgtccac ttaccactat cttaatttcc ccctctcccc accgtacccg
    21901 ggagctgcat ggaggacagg agtgtcgggc gcatcgccct cttcatcgcc atccttgcag
    21961 ggttcctcgg cccctttgac atctcggcgg tgaacatcgc cctccccacc atcggggcgg
    22021 agttctccct ggacgccctc gccctctcct gggtgagcac cgcctacctc ctctcctcgg
    22081 ccctcttcct cgtccccttc ggccggatcg ccgacatcgt ggggaggaaa aggatcttca
    22141 ccctcgggct ctctctcttc accgcggcat ccctcgcgat ggccctcacg gtgatgcctg
    22201 cgaccctcat ccccctccgg gtgatccagg gaatgggggg cgccctcatc tacgggacgg
    22261 cagtggccat cctcacggcg gtcaccccgc cggaggaccg ggggaaggcc cttggcatct
    22321 acacgacggc ggtctatgtg gggctctcca tcgggccctt tctcggcgga ttcatgaccg
    22381 gggccttcgg ctggcggtct atcttcctcg tgaacgtccc caccggcctc ttcgccctct
    22441 ggctcgtccg tacccggctc cacggcgagt gggctgatgc ccgcggggag agcttcgata
    22501 cccccggatc cctcctctac ggcctctccc ttgtcgccgt catgctcggc ttcacctggc
    22561 tgcccggccc cccggggatc gtcatgatcc tcctcggggt cctcctcctc gccgggttcg
    22621 tgcggcggga gcagggaacc cggcatcccc tcctcgacgt ggggctcttc tcggggaacc
    22681 gggtctttgc cctctcgaac ctcgcggccc tcatcaacta cagcgccacc tttgccgtga
    22741 ccttcttcct ctccttctac ctccagtcca tccggggctt ctccccggcg gcggcggggg
    22801 cgatcctcgt gacccagccg atcctccagg cggttttctc ctcgcccgcg ggccggctct
    22861 cggaccgggt ggagcccggg aaggtcgcct cggcagggat ggggctctgt gccctcggtc
    22921 tcctcctcct cgccttcatc cgggaggaca cacccctctc cttcctcctc ctcgccctcg
    22981 ccctcctcgg caccggcttc gccctcttct cctccccgaa cacgaacgcg gtgatgtcct
    23041 cggtggagag gcggtgctac ggggtcgcct cggggaccct cgggaccatg cggtacgtcg
    23101 ggcagatgct ctccatgggc atcgcgatga ccctctttgc cgtccacatc ggccgtgtcg
    23161 agatcgggcc cgcgaactac ccggggctcc tcgccgccat ccaggctggc ttcctcatct
    23221 ttaccatcct ctgtgtcatc gggatctacc cgtccctcgc ccgggggaac atgagggacc
    23281 aggagaaggc cggctgcagg gtttcgtgag ggagggtcca tcaggacccg acctcgagcg
    23341 acgaagtcgt gaagagggag gtttcgaaag agggaggttt cgtcaggaac cgaccttgag
    23401 cgacatagtc gtgaaaaacc gacctggagc aacccgctgt aatgcggggt gcgaccctga
    23461 ggacggtcta accctctccc tcgacaaccg acgtgaaccc gctgcggtcc tcccagccga
    23521 tcctcagggt ggcattggca aggatgagcc ggtcggtctg tgaccagacg gtcagcgtga
    23581 tgtttacggg ccgcctcgcc ttctccccga gctccgagaa gtaggaccag tagactggtt
    23641 cttccgtccc ggtgtacctt gccccaagct cggtgacctt gaagtcgggt ggcccccagc
    23701 ggtagaagct cccaaaggtc gtctcccagg tgaaccgggc atccggcgtg atgataccgg
    23761 aggtgttcac cggggtcagc cggatgccga tggtggagga catcgccggg ttgtagcggg
    23821 gaggggtggc gctgatgcgg atggtgaggg ggacggcgac ggtcgggatg ggggatggaa
    23881 cttgttgcga gccgtgtgtg gcggggatga ggcagccgca ggcgacggtt atcagaaccg
    23941 tagagacgac aaagagaaaa gcgtcccttt tcataaaatt gtcacttgag gattcgatgg
    24001 ggcagagaga aaaagatgcc gatgacaaga cgaaaactct cgggcagtcg gacgctcctg
    24061 ggggaaggga gggttcatca gaacccgacc tggagcaacc cgccataagg ctgggtgcga
    24121 cctggaggtt tcgtaagagg gagggttcgt aggacccgac ctcgagcgac gaagtcgcga
    24181 agaaaccgac ctcgagcgac gaagtcgcga gttcgctttg ctcacccccg ccgctctggc
    24241 gtcccccgaa atgcgatagc ccgatcatcc atgtaacctc ccctcctagg aattccggac
    24301 ccgggggggg tgcccatgcg gcggggggcg ggtgagcccc ccgagagcat cccctaagaa
    24361 acatatgctt atcccagtta gcaatcggcc gattatctct tttcagaagg ccgcgacgaa
    24421 tcgaatcaat taactggaaa ttccggaaga aaaaagagat gattacccga agagggctcc
    24481 gagccctgcc atcgcggact cttcctcctg cttcttgtcc tccttcttct cttcgtgggc
    24541 cgctgccgtc gccgcagccg ccggtgctgc agccgctgcc ggggccggtg cggcggccac
    24601 gggcgccacc gcggccttcg cgatggcctc ggtgatgttc actccctcga gtgcggcgat
    24661 gagggccttc acccgggagt cgtccaccgc gacgcccgcc gcggtgagga cgctctttac
    24721 gctcgtctcg tcgatcttct ttcctgcgtg atgcaggagc agtgcagcat agatgtattc
    24781 catgtcagtt caccttttcc agtaactccg ttccttattt tccacctacg attcctttca
    24841 gggtcttcgc ctcgcgcgac gcccgcgcga tgatggcgtc catggcgtcc ttttcgtaga
    24901 cgctcgcctc gacggccagg ttctttgcct cggagaaggc cttcccgatg atggccgcaa
    24961 cggtgacctt cgtcgggtac gcggcattca ccgagaggtt gaaggcctgc cgtgccgcga
    25021 ggatgatgtc cctccggtac gcctcctcgt cgatggcgag catctcggac cggaagacgg
    25081 ccttctcgta aaaggccacc tggagccgga ggcccacgtc catgggcttg atgtcgagct
    25141 tcgagaggac gtccgccacc ttcttcgaga tcacgtcccc cttcttcacg acgaccttcg
    25201 tgtcccggat cctcaccttg ccggcctcga tgaccgccgg gatccccgcc tgctggagct
    25261 ccccgacgat cgggccgggc ttgaagttcg tggggcccct gggaacgacg atgtcctggg
    25321 gggcgacctc tcccggcttc gccgccatct tggtcttcgt cctctccagg gtcttataca
    25381 gggagaaggg gttctggtcc gtgaagatga gggcgctctg gccggcgatg aacttctcga
    25441 ggtcggcgta gtcgcctccg agttcggcga aggcatgctc gatgagggtg ttcctcgtca
    25501 tcttgagcac cgccttcccg tggaggtccc tccggatccc ctggagctgg gccgccggga
    25561 tgccgtggat gtccacgagg gagagcaggg cgtattcctt cgcccacgtc ttgatcttct
    25621 ccacctcgtc ccgcttccac tgggggaggt ggtgcgtgta cagggccatc tcacaccatc
    25681 ctcacggcgg ggcccatggt ggtcttcaca tagaccgacc ggatgttcag gggtccctgc
    25741 tcgagcacgg actcgatcct cttcatgacc acatcgatgt tctcggcgat caggttcggg
    25801 tccatctcgg tggagccgac ccgggcgtgg aaggtggtcc ggtccttggt ccggatcttc
    25861 accgacgcac ggagccggtc caccatcggc cggatatcgg ttccgggcgg gacgggggtg
    25921 ggcatcctcc cccggggccc gagccggggc ccgaggaacc tgcccacctg ggacatgacc
    25981 gatgtctctg cgatgaagaa gcggtactcg gccgcgatct tccgtgcctc gcggggtgcg
    26041 cctcccaggc gctcgatctc ctcggaggag atgatcaggt caaccttcgc gtccttcgcc
    26101 cgggaggtga tatccccctt cccgagcacg gcgatcttca ctccgcccag ctggtggggg
    26161 aggaggatgg tttcatcgat ccggttcttg ggctgggaca aatcgatgtt ctttaggttc
    26221 accgtgatgt ccacgctctc ccggaacttg cgcttcgggg cgctctcgat ggcctgcttt
    26281 acggtggctg cgatcttttc cttctcaacc atctgatacc tccatagttc tcgacctctt
    26341 ccgatctcct atggtaagtc tccttcatgc gaggcgcgca tcgaactcgc ccgcatcgat
    26401 ggccgcaagg atctccttcg gcctcctgcc ctccacggtg actcccatgg agacacaggt
    26461 gccgacgacc tcccggacac gcttcttcgt gtcataggat agcatgtcgc cggccttcat
    26521 gcgggcgatc cggatggctg cctcaagcgg caggttcccc accagctgcg tgctgggctg
    26581 ggccgagccc ttctcgatcc ccgcctcctt cttgatgagg gcggtggtcg ggggggtccc
    26641 cacggtcacc gtgaactgct tcttgtcgtc gacctcgatc ttgaccggca cctgcatccc
    26701 gttgaagtcc gcagttctcc ggttgatctc gtccacgacc gccttcacgt tgataccgag
    26761 gggccccaga gcgggcccga ggggctggcc gggtgtggcc ttgccgccgg gtaccagtac
    26821 ctcgacgatc tctcccatag cgtttccacc aaggctcggg accctgaatc gggttcggcc
    26881 gttgggttat ataaggtcgt gccgaggggt taaaaacgtt tcaatcgggg gattcctggt
    26941 cctctacctt cgcggaaatc ctgcggattt cactctggcg tcggacctac gggtcctcca
    27001 cctggtggcg acttacgtcg cccaccagtc gaagatccta ttgatcttcg accttcgcga
    27061 aatccctcgg atttcgctct agtctcggat cttactgatc ttcgacttgg ccggaaattc
    27121 tgatgaattt ccgccggtgg agggcctatt ggtcctccac cttctcgacc acccggacat
    27181 ggtcgccgcg gaccgtgatg gggatgggga ccaccgactc gtagagctcg acggtgatct
    27241 cctccttgcc catgtccacc cgcttcacca ccgccttctc tcccttgaag gggccggcga
    27301 tgagctcgac gatggtaccc tcctcgatcc cgctcaccac cggcttcggg acgaggaagt
    27361 gggccacctc ctcgatcttc gtctctccct tcaccaccgc gcgcgcgtgg gggatcatct
    27421 ccacgagctg ctcgatccgc gcgtggctct cgggagtctc cacgagcacg tagccccgga
    27481 gctcgtcggg gacgatgatg gccaccacct tgatgtcggt ctgggtctcg acggccttct
    27541 tgatgctgtc ggccacggca cgttcctgct tcgccgtcgt cttgatggcg aagatgcggt
    27601 tccccgcggt cgccgggctc gcgggctcgg ccggggccgg tgcgggtccc tgggggggct
    27661 gggctgcctc gtcggtcatg gaacctccgt cagtggggga gaacacccat gaacgagagg
    27721 atcaggaaac cgatgagccc gatgaggatg atccccgcgg cggcgacgat ggctatcttc
    27781 tggaattcct cccgggtggg ggtccgcgcg agcttgatga cgcgccagta tttgtggaag
    27841 agctcttcct ctagcttgaa tgccatctgt tcctcacttc atgaagtcta tctcgaaatg
    27901 ctttgcgacc ctgcgggggg cctccttctc ggccctgccg tagatctggg gtgagtggac
    27961 acctgtgacg acgagcatgg tcctcatccg gtgctgcatg gtcgggtcca ccatggcacc
    28021 ccagatgatc cgggcgttcg ggtccacccg gtcgaagacc tcctggacga ccccctcggc
    28081 ctcggcgatg gtcatgtccg ggccgcccac gacgttcacg agggccgccg tggcccccga
    28141 gatgtccacg tcgaggaggg gggaccggag ggccttcttc accgagtcgg ccgccttgtc
    28201 ctcggcatcg ctctcgccca tgccgatcat ggcgaccccg ccccgctcca tcacggtgcg
    28261 gacatcggcg aagtccaggt tcacgagccc ggggagggtg atgagctcgg tgatgccctt
    28321 cacggcccgc atgagcacct cgtcagcgac cttgaaggcg gcgtagaggg ggagccgggg
    28381 gacgacctcg aggagccggt cgttcgggac cacgatgacc gtgtcggcga tgtcccggag
    28441 gcgctccagg cccgcctcgg cgttctcccg ccggatagac ccttcggcgg tgaaggggag
    28501 ggtgacgacg gcgatggtaa gggccccctg ctcccgggcg gcctttgcca cgacgggcgc
    28561 cgcccccgtg ccggtcccgc cgccgagacc ggtggtgaga aagaccatgt cgcagcccgc
    28621 gacgatcttc ttgatctcct ccaccgactc gacggccgcc tcctccccta cctgggggat
    28681 ggacccggcc ccgagccctc tcgtcctctg ccggccgagg aggattcgat actgggcctg
    28741 ggtgcggatg aggtgctggg catccgtgtt catggcgacg agccgggcgc cgtggattcc
    28801 ctcctccatc atgcgggaga tggtattgga cccgctcccc ccgcagccga tgaccgcgat
    28861 ctctgtcttc agctgggaaa ggacctcctc gagctccttg tccggcatgg gggcggctat
    28921 cggcacgacg ggcggctgtg ccccggccgc cgtggccttc tgttcctcct ctccccgtgc
    28981 caacgcctcg tcgatgatgg acttcatagg tacaactcca accccgggat atgtatctct
    29041 gtaaccctgg ctgtctgga
//
LOCUS       DATGDI010000199         3850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_369834, whole genome shotgun sequence.
ACCESSION   DATGDI010000199 DATGDI010000000
VERSION     DATGDI010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369834"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1809
                     /locus_tag="VKO45_07465"
     CDS             <1..1809
                     /locus_tag="VKO45_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HMA05749.1"
                     /translation="EFEGPELVIYTEDPKKFADQADLIKILARDLRKRIVVRPTILED
                     PDRAIGRINEVVPETAGITDLFFDADTGEVLIEAEKPGVVIGKNGATLREITKEIGWT
                     PKVVRTPPIESTTIKQIRQYLRSVKEERKIFLRAIGRRIHRETTSKDEWVRVTTLGCC
                     REVGRAAFLISTPESKILLDCGEKPSNANGTPYLYVPEIHPLSGLDAVVLTHAHLDHC
                     ALIPLLFKYGFDGPVYCTPPTRDLSAMLQLDYLEVIQKEAGKTPYTSTDVKNFIRHSI
                     SLNYGAVTDIAPDIKLTFHNAGHILGSAIAHFHIGDGLYNIAFTGDFNYSKSRLFSPA
                     ASQFPRLETVFMESTYGGQNDIQPPRREAELKLYEVVNTILSRGGKVLIPAFAVGRSQ
                     EVMLALEEGMRMGKIPRVNVYLDGMIREATAIHTTYPEYLNADLRNQIFHDGMNPFLA
                     PCFVQVDSSERREQVMGGDPCVIITTSGMLNGGPVMEYLKGLAPDARNALVFVGYQAE
                     GTIGRRIQKGWREVPLGGRETIAINLEIDTVDGFSGHSDRRELMNYIGHLQPRPEKIF
                     TIHGDENKTIDLAVSLHKRYHIETHSPLNLETYRMI"
     gene            complement(2066..3118)
                     /locus_tag="VKO45_07470"
     CDS             complement(2066..3118)
                     /locus_tag="VKO45_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HMA05750.1"
                     /translation="MKAAIYYSNSDIRIEDVEDLPVGPGEIKVKIMACGVCGSDVMEW
                     YRMKRAGRPGGIGAFGHECTGIIAEIGPGVDLKWNVGDRVVVTHHVPCNTCNACVRGH
                     TTACDTLQKTKFKNAFGAFAEHVVLPAINVDRGMLPLPESVSFDLGTFVEPLGSVLRG
                     QHWAPTSDGRSVLIIGAGITGLLHIQAARLNGAGFIAVSNPSPERLRIAKKFGADATI
                     SATEDVPGRFKELNGGLGADTVIMTAPVPVCVKQSLEAVGPGGTILFFAPTRPEIQSE
                     INLWNLWQKEVTITHSYGADFHNLSTALKLIQYKRVNVTDMITHVFPLQKTADGFLLT
                     ARHRDGSLKVIIHPQE"
     gene            complement(3161..>3850)
                     /locus_tag="VKO45_07475"
     CDS             complement(3161..>3850)
                     /locus_tag="VKO45_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05751.1"
                     /translation="SKTIKTLLPYCDCLMTTRGAVRSNIAPEDVGSKPIMLRVTGGNS
                     VSNEELSDEKITVTIQEAIRMDAAGVAVSVYIGSANQQQTILNLTDMINQAEEYGIPV
                     LAVTAVGKNMARDLRYLALASRICQDAGARIIKTYYCEEFSRLVDAVTPTAVVVAGGK
                     YSSPPDALQMAYNSVQAGAVGVDFGRNIVQDDNPVGMIKAIGTIVHGNHTVKEALDIY
                     NASLPHASRLD"
BASE COUNT          837 a         1196 c         1034 g          783 t
ORIGIN      
        1 gagttcgagg gccccgagct cgtcatctac accgaggacc cgaagaagtt cgcggaccag
       61 gccgacctca tcaagatcct ggcccgcgac ctccggaaga ggatcgtggt acggcccacc
      121 atcctcgagg atcccgacag ggccatcggc aggatcaacg aggtggtacc cgaaaccgcg
      181 gggatcaccg acctcttctt tgatgcggac acgggcgagg tgctcatcga ggccgagaag
      241 cccggggtcg tcatcggcaa gaacggggcg accctccggg agatcacgaa ggagatcggc
      301 tggaccccga aggtcgtccg gacgccgccc atcgagtcca cgaccatcaa gcagatccgg
      361 cagtacctcc ggtcggtgaa ggaggagcgg aagatcttcc tccgggccat cggccggcgg
      421 atccaccggg agaccacctc gaaggacgag tgggtccggg tcacgaccct cggctgctgc
      481 cgggaagtgg ggagggccgc cttcctcatc tccaccccgg agagcaagat cctcctcgac
      541 tgcggcgaga agccgagcaa tgccaacggc accccctacc tctacgtgcc cgagatccac
      601 cccctctccg ggctcgacgc cgtggtcctc acccacgccc atctggatca ctgcgccctc
      661 atccccctcc tcttcaagta cggcttcgat ggcccggtct actgcacccc gccgacgagg
      721 gacctctcgg ccatgctcca gctcgactac ctggaggtca tccagaagga ggcgggaaag
      781 accccctaca cctccaccga cgtgaagaac ttcatccggc actccatcag cctcaactac
      841 ggggcggtga cggacatcgc cccggacatc aagctcacct tccacaacgc cgggcacatc
      901 cttggctcgg ccatcgccca cttccacatc ggggacggcc tgtacaacat cgcgtttacc
      961 ggggacttca actacagcaa gagccggctc ttctcccccg cggcctccca gttcccccgg
     1021 ctcgagaccg ttttcatgga gtccacctac ggcggccaga acgacatcca gccgccccgg
     1081 agagaggcgg agctcaagct ctacgaggtc gtgaacacca tcctctcccg ggggggaaag
     1141 gtcctcatcc cggcctttgc cgtcggccgg tcccaggagg tcatgctcgc cctcgaggag
     1201 gggatgcgca tggggaagat cccgcgggtg aacgtctacc tggacgggat gatccgggag
     1261 gcgacggcca tccacaccac gtaccccgag tacctgaacg ccgacctgag gaaccagatc
     1321 ttccacgacg ggatgaaccc cttcctcgcc ccctgcttcg tgcaggtcga ctcgtccgag
     1381 cgccgggagc aggtcatggg cggcgacccc tgcgtcatca tcaccacctc ggggatgctg
     1441 aacggcgggc cggtcatgga gtacctgaag ggcctcgccc cggacgcgag aaacgccctc
     1501 gtcttcgtcg gctaccaggc cgagggaacc atcggccggc ggatccagaa gggctggcgg
     1561 gaggtccccc tcgggggccg ggagaccatc gccatcaacc tggagatcga caccgtggac
     1621 gggttctcgg gccactcgga ccggcgggag ctcatgaatt acatcggcca cctccagccc
     1681 cggccggaga agatctttac catccacggc gacgagaaca agaccatcga cctcgccgtc
     1741 tccctccaca agcggtacca catcgagacc cactccccgc tgaacctcga gacctaccgc
     1801 atgatctgag atgagggaga gtctcccccc tctatctcgg ggtcttgtag tcccagacct
     1861 ttttcaccaa tctattggca ttacgagggg tgggaaatgg ggcacgcata ccctccggat
     1921 catcataggg cgaggtgttc cccaccttga tgtggtctca agtatcgaag ctcgaccacc
     1981 ccctctgttg gatgcacgtg tcaccttcgg tgaccgaggg tcatttctca tgaaatgccc
     2041 catttgagaa attcgaaaaa aaacgttatt cctgcgggtg gatgataact tttagactgc
     2101 catcccggtg tctggcagtc aggagaaacc catcggccgt tttttgtaag ggaaaaacat
     2161 gggttatcat gtcggttaca ttcactcttt tatactgtat caatttcagg gccgttgaaa
     2221 gattatggaa atcggcacca taggaatggg taatcgtcac ctccttttgc cacaggttcc
     2281 agagatttat ttcggactgg atctcgggac gtgtgggggc aaaaaacaag atcgtacccc
     2341 ccggtccgac tgcttcaagg gactgtttca cgcaaacggg aaccggtgct gtcataataa
     2401 cggtatctgc gcccaggcca ccgtttaatt ctttaaacct tccgggaaca tcttcagtgg
     2461 cggaaatggt tgcatcggca ccgaattttt tagcgatcct caaccgttcg ggactcggat
     2521 tggagacagc aataaatccc gcaccattca gacgggctgc ctggatatgc aacaatccgg
     2581 tgataccggc accgatgatc aggaccgatc gaccatccga ggtcggagcc cagtgttgcc
     2641 cacgcaaaac acttcccagg ggttcaacaa aggttcccaa gtcaaaagaa accgattcgg
     2701 ggaggggtaa catgccccga tccacattga tcgcgggcag aaccacatgt tctgcaaacg
     2761 ctccgaacgc atttttaaat tttgtctttt gcagggtgtc acaggcggtt gtatgcccac
     2821 gcacacaggc attacaggta ttgcatggta catggtgggt aaccacgacc cgatctccga
     2881 cgttccattt cagatctacg ccgggaccga tttctgcaat aattcccgtg cattcatgtc
     2941 caaacgcccc gataccaccc ggtcttccag cccgcttcat ccgataccat tccatgacat
     3001 ccgacccgca cactccgcat gccatgatct tgaccttgat ctctcccggc ccgacgggga
     3061 gatcttcaac atcttcgatg cgaatgtcac tgttcgaata atagatggct gctttcattc
     3121 tgttccctgt ggagagtaca tgtctatatg tttgaaaaat ttagtcaagt ctggatgcat
     3181 ggggtagact tgcattgtag atatccaggg cttcctttac ggtatgattc ccatgaacaa
     3241 tggtcccaat tgctttgatc atgccgaccg gattgtcatc ctggacaata tttcgtccga
     3301 aatcgactcc cacggcaccc gcctggacag agttgtaggc catctggaga gcgtcaggag
     3361 ggctgctgta cttgcctcct gcaacgacga ccgcagtggg agtaacggcg tcaaccagtc
     3421 tggaaaattc ctcgcaataa taggttttga taatccgggc cccggcatcc tggcagatac
     3481 gggatgcaag agcgagatag cgcaaatccc gggccatgtt tttacccacg gctgttacgg
     3541 ccagaacggg gattccatac tcttcggcct ggttgatcat atcagtgaga ttgagaatcg
     3601 tctgttgctg attggcagag ccgatataca cgctgacggc gacaccggcc gcatccatcc
     3661 ggatggcttc ctgaatggtc accgtaatct tttcatcact taactcttca tttgaaacgc
     3721 tgtttccccc cgtaacccgc aacatgatgg gttttgatcc gacatcctcg ggagcaatat
     3781 tgctccgcac tgccccgcgt gtcgtcatca ggcaatcgca atagggaagc agtgttttga
     3841 tggttttact
//
LOCUS       DATGDI010000200         1532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_399259, whole genome shotgun sequence.
ACCESSION   DATGDI010000200 DATGDI010000000
VERSION     DATGDI010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399259"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1532)
                     /locus_tag="VKO45_07480"
     CDS             complement(<1..>1532)
                     /locus_tag="VKO45_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HMA05752.1"
                     /translation="VFSHDVGDAHVRGDLHLHDLGYVDRPYSSSQTLEFVKKFGLNLP
                     NTFSASKPARHAEVLLAHMVRYSAVLQGYFSGTIGWDAVNVFFAPYLEKLSDREIEQI
                     AQMLVFEFSQLAVSRGGQAIFTEIGIYWEIPRHLRDVPAIGPGGELTGKSYGHYAKDA
                     QRFAGALFEIYREGDALGSPFFFPKPLLRMTEDFFETPGHEDFLLQVCGAAAEKGNPH
                     FIFDRNGTLKVPRCLSLDFASDGQARREAQKPWEMRYASIQNVTLNLPRLGYLAEGND
                     THLFSLISERMEIVARAHTQKRQFIERLLAIGDGGPLALLSMDCVGSAYLKLDRATCL
                     IGMVGLNELVQLHRGAQLHEDEKALKFGLKILGHIQKMAARLGRQHSMRFILEQTPAE
                     STAYRFARLDLKYHSPRSGHLVRGDIARGEMYYTNSTQLNIAAKTDPFGRVRSEGLFH
                     PLIDGNAITSIWLGESRPSAEALARFITRAFRETTSSQIVFSPEFTVCPACRQTVRGL
                     KA"
BASE COUNT          273 a          463 c          507 g          289 t
ORIGIN      
        1 cgccttgaga ccgcgcaccg tctgccggca ggcgggacag accgtgaatt cgggcgagaa
       61 gacgatctgg ctgcttgtcg tttcgcgaaa ggccctcgtg atgaatcggg caagtgcctc
      121 ggccgaaggt ctggactcgc caagccagat cgacgtgatg gcattgccgt ctatgagggg
      181 gtggaagagg ccctcgctcc gcacccgacc gaaggggtcc gtcttggcgg cgatgttgag
      241 ctgcgtcgag ttcgtgtagt acatctcgcc ccgggcgatg tctccccgga caagatgtcc
      301 cgaccggggg gagtggtact tgagatcgag cctggcgaac cggtaggcgg tgctctcggc
      361 aggggtctgt tcgagaatga accgcatgct gtgctgccgt cccagcctgg ccgccatctt
      421 ctggatgtgg ccgaggatct tgagcccgaa tttcaatgcc ttttcgtcct cgtgaagctg
      481 tgcgccccgg tggagctgca cgagctcgtt gagccccacc atcccgatga gacaggtggc
      541 ccggtccagt ttcagatagg ctgaccctac gcagtccatg gacagcaggg ccagggggcc
      601 tccgtcaccg atggcgagga gcctctcgat gaactgcctc ttctgggtgt gggccctggc
      661 gacgatctcc atccgctccg agatcaggga aaagaggtgc gtgtcattgc cctcggcaag
      721 gtatcccagc ctcgggagat taagcgtcac gttctggatg gaggcatagc gcatctccca
      781 gggcttttgc gcttccctgc gggcctggcc gtcgcttgcg aagtcgaggc tcaggcaccg
      841 cggaaccttg agggtcccgt tgcggtcgaa gatgaaatgg gggttcccct tctcggcggc
      901 cgccccgcag acctgcagga gaaaatcctc gtggccgggt gtctcgaaga agtcctccgt
      961 catcctcaaa agcggcttcg ggaagaaaaa ggggctcccc agggcatcgc cctcgcggta
     1021 gatttcgaag agggccccgg caaagcgctg cgcgtctttt gcgtagtgtc catagctctt
     1081 ccccgtgagc tcccctccgg ggccgatggc gggcacgtcg cgcaggtgcc gggggatttc
     1141 ccagtagatc ccgatctcgg taaaaatggc ctggccgccc cgcgagacgg caagctggga
     1201 gaactcgaag acgagcatct gggcgatctg ctcgatctcc cggtccgaga gcttctccag
     1261 gtagggggcg aaaaagacat tcacggcgtc ccagccgatg gttcccgaga aatacccctg
     1321 caggacggcg ctgtagcgga ccatgtgcgc gagcagcacc tccgcgtgcc tggccggctt
     1381 cgaggccgag aaggtgttgg gcaggttgag gccgaacttc ttgacgaact ccagggtctg
     1441 gctcgaactg tagggccggt ccacatagcc caggtcgtgc aggtgcaaat cccctctcac
     1501 gtgggcatcc ccgacatcgt gggagaagac cc
//
LOCUS       DATGDI010000201         9937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_312295, whole genome shotgun sequence.
ACCESSION   DATGDI010000201 DATGDI010000000
VERSION     DATGDI010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312295"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VKO45_07485"
     CDS             <1..523
                     /locus_tag="VKO45_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05753.1"
                     /translation="KNALSDAENALISSPTQVQSLLDTATANINAGQTALDQADADNT
                     LQKARAMIISVDGLLKEFTVNDSLKASDPRLVPIINKRDLAAQAIASANESFTSGSYP
                     AARGKANDGLALANQAWNLSLDLKTELGQGFQLPGLPNLSAFLPVLIVIVVVAIIAGV
                     IIYRKKMHWDELG"
     gene            complement(655..2262)
                     /locus_tag="VKO45_07490"
     CDS             complement(655..2262)
                     /locus_tag="VKO45_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HMA05754.1"
                     /translation="MRRETAWLTERLRSIFTFDRIFLLILVLTVLLRFSQLDLKLFHH
                     DEAIHGWFSYRLLHEGIWTYDPSYHGPLLYYATAGMFWLLGDGDVVGRLIPSLLGTLI
                     VALVYPLYRMGYLDRRQTLVGALFLALSPDLVYFSRFLRHDIFMLFFIMLLLVTLLAY
                     IETGSRWYGLGVAVAVAGGMACKEEFPVILLIFGIYFIYAAWRGRIRFPSRWRVDLAL
                     FTVVALGIIAFFYSSLGQHPEILLDAAQRAIGHWLEIHEACRLCGPWYFYLLLFAIYE
                     LPILILAIAGTLQFLAARTDLRGWWEDLRRRIRREEPSTETLLARLGAAPNALSPMEA
                     KREEFFGFCIWWMVLSMAFYAIVNEKVPWLIIPQLLPAVFVAMYRLTDTKTIIAIVSV
                     AFLVLATWHTSFVPADVNEPIVQVQNSEDLRGLFSQIDNSSRVAVASENYWPLPWYYR
                     GVIAKKLAYYGKRVDEATIYDNNYDLVIAYDQSSYSFLDGYEKRTIRLNYWFSSYDNQ
                     DRLLAWYFLRDGKMGSMNLDIFTREVS"
     gene            complement(2265..2573)
                     /gene="rpsJ"
                     /locus_tag="VKO45_07495"
     CDS             complement(2265..2573)
                     /gene="rpsJ"
                     /locus_tag="VKO45_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HMA05755.1"
                     /translation="MQKARIRLTGTDCKKVEMVCERIREIAERTGVNLAGPIPLPTRR
                     LVVPVRKSPDGEGTATWDRWQMRIHKRLIDIDADERALRQLMRIQVPKDIGIEIVLES
                     "
     gene            complement(2592..3869)
                     /gene="tuf"
                     /locus_tag="VKO45_07500"
     CDS             complement(2592..3869)
                     /gene="tuf"
                     /locus_tag="VKO45_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HMA05756.1"
                     /translation="MAAAKPHLNLAIIGHIDHGKSTLVGRLLFETGAVPPHVIEQYRK
                     EAESKGKGTFEFAWVMDNLKEERERGITIDIAHKRFDTAKYYFTVVDCPGHRDFVKNM
                     ITGASQADAAVLVVAASEGVQEQTKEHVFLARTLGINQLIIVINKMDAVNYDQKRFDA
                     TKTDVTALLKMVAYKPDDMLFIPASAFQGVNISKKSDKTPWYKGPTVLEALDTLKEPE
                     KPTTLPLRLPIQDVYSISGIGTVPVGRVETGILKKGMNVIFMPANKTGEVKSVEMHHE
                     EIPQAVPGDNVGFNIRGVAKNEIRRGDVVGPVDNPPTVADEFTAQIVILYHPSAITVG
                     YTPVFHCHTAQVACTVTALKKKLDPRTGQTKEENPTFLKTGDAAIVQIKPSRPLVIEK
                     IKEIPPMGRFAIRDMGSTIAAGMCIDIVPKMMR"
     gene            4112..4417
                     /locus_tag="VKO45_07505"
     CDS             4112..4417
                     /locus_tag="VKO45_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05757.1"
                     /translation="MKVLSFSRSPLSERVTVQCADGEVTVHSHCAYCTHCAGVQIGKR
                     SMPSPILSAYKDVRRGTAPDEALMNAGMMFNTLIRDGDAINCDDDGNEGFSSMYHHC"
     gene            complement(4408..5268)
                     /locus_tag="VKO45_07510"
     CDS             complement(4408..5268)
                     /locus_tag="VKO45_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA05758.1"
                     /translation="MGPHYGIHLRGGVITERDVDLCTIRVRVPAGNLTVEQMRGLANI
                     SKRYGKGFVHFTGRQAAEIPHVNPKHLVRITRALAKNGTPLGSEKDEVVNIVACPGKE
                     RCKYGMVDTISLARKLDERLFGKEMPVKMRISISGCPNACTSPMLNEIGIIGRVKPRR
                     IPGLCTGCGTCAEYCKECAISIRNGISVLDEEKCVQCGVCIQSCPFHLLKSEHTHYLV
                     MVGGRRGRHPRTGRTLLAIEEEDKVVEAVERIIQWVYRRAWSGRLLSDQLDDLHFEGF
                     RNEIAGQLQQ"
     gene            complement(5316..6032)
                     /gene="cobS"
                     /locus_tag="VKO45_07515"
     CDS             complement(5316..6032)
                     /gene="cobS"
                     /locus_tag="VKO45_07515"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HMA05759.1"
                     /translation="MILDPVIALLQFETRLPLGRTVDLGAFARHPWLYPLAGYVSGGI
                     AALAAFLIAPRPLGAAIALSLALLVTGFHHLDGLLDFGDGLMAHGSREKRVAALTDRA
                     IGAGGIGLGLVVTLVSFAALQSVPSAWIAILAGEVLARLGTAWITVAGRPFREGIHAT
                     LHGGARPVHGLYAPVPALPLLLLLPSGSLLLAGAVTAITVAAMIALGDHLFGGVNGDL
                     AGATHEIVRALVIASLVLIP"
     gene            complement(6029..6463)
                     /locus_tag="VKO45_07520"
     CDS             complement(6029..6463)
                     /locus_tag="VKO45_07520"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HMA05760.1"
                     /translation="MFGIAERLEAKGIDLPSMVEAAMALYVPHGMSPEEAGGVVARKL
                     EKAMRDPNVSSLLLAAVLLEEELCTGRPGTIGGDPVFLLSDEILGMAIAEYIGGTCAR
                     FEFTRYDQKKPGILSRLGPFLDDAVAGLIAGCTSRLYTECVE"
     gene            6507..7514
                     /locus_tag="VKO45_07525"
     CDS             6507..7514
                     /locus_tag="VKO45_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HMA05761.1"
                     /translation="MPFLSGTPPFRPKCPVFAIVLGNTLLSTVPGVSGAGMSPEKTVL
                     TPILDSELITRGVIRSRRWKPNTPTGCPTPAVITRAMMVLLGMEPVFVNAGLTTKPVV
                     PCLDVYGEPGRDPRDGDAVPMARELYARGEQVGRLLSRAGDLLLLGECVPGGTTTALC
                     VLRGLGIPASVSSSFVDNPVALKEAICARVQERIRAETPTDPLDVIRIGGDPMLAVAA
                     GIAASFRGDTVLAGGTQMLAVAAVLRDLGKKVPPIVTTVYVRDDPSAGFAATAGMVGA
                     QVYYIDPDFGGIGHPGLARYCAGEVKEGIGAGGAMFLARIMGRSEEEIRETILSTASS
                     Y"
     gene            7551..8108
                     /locus_tag="VKO45_07530"
     CDS             7551..8108
                     /locus_tag="VKO45_07530"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase subunit A"
                     /protein_id="HMA05762.1"
                     /translation="MRPLYVVNNFGQFNHLILRSLRDMGIPAELVPNTTPTGEVAGGC
                     RGIILGGGPSMERAGNSARYLELGLPVLGICLGMQAMALARGGKVSPGSMGGYGLVEV
                     EISEPDEILAGYPSATSVWASHMDEVTRMPDGFTLLASSRICSIEAMARPSEHLYGVQ
                     WHPEVSHTVNGRLTFQNFDRICGGA"
     gene            8128..8688
                     /locus_tag="VKO45_07535"
     CDS             8128..8688
                     /locus_tag="VKO45_07535"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMA05763.1"
                     /translation="MKDSPGTPDLADIAADIRRIGFSCLNCGSCCRSGPDDPGLVFAS
                     HAEIEALVSSGGGAWEEVAVPYPEFIPCGDGSAVTFGWCLRQEEGRCRFLSDGSCRRY
                     RARPWICRTYPFALVDGELTVSDCPGLGELVSEEDALALAEALLERARCEADDEDRVR
                     KIFTSERIPKGKRCVVDASGITVCPD"
     gene            8759..9265
                     /locus_tag="VKO45_07540"
     CDS             8759..9265
                     /locus_tag="VKO45_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMA05764.1"
                     /translation="MNVGTTIIKGRRSIRSFRDTEIPEDAIRDALECARQAPTAMNGQ
                     PWLIGVVEEKETLQKIAGLTDYGKHIAGARACFAVFGEKKAKYYLEDCCCATMNLILG
                     LWAYGIGSCWVAGDKKPYAGAVRELLGVPDSYTLVSLVPAGYPADMTLPPKKELSAIS
                     FSERFVAR"
     gene            complement(9227..9814)
                     /locus_tag="VKO45_07545"
     CDS             complement(9227..9814)
                     /locus_tag="VKO45_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 domain-containing protein"
                     /protein_id="HMA05765.1"
                     /translation="MDKESMRTRIAVLSARMRQAKSKEALAAAVADEIATYTLYDLLR
                     LRAFVERDLRHVPPHYRSRLQPRMMDHLFGTHHAIILAHRRGRFEDLQDPPEGKLAEF
                     CDMLLGLPDREEGDLRLVLFYYLIAAFTIFVRELPGHPVGTPFPGGFIVEMRDGVYYC
                     PVREKEEDVETSICPFCPAKQSVLPGDEPLREADG"
BASE COUNT         1742 a         3089 c         3327 g         1779 t
ORIGIN      
        1 gaagaatgcc ctgagtgatg cggagaatgc cctcatctcg tcgcccaccc aggttcagtc
       61 ccttctcgat accgcgacgg caaatatcaa tgcaggacag accgccctcg accaggcgga
      121 tgccgacaac accctccaga aggcgagggc gatgatcatt tcggtggacg ggctcctcaa
      181 agagttcacg gtgaatgaca gcctgaaggc gtcagacccg aggctcgtac ccatcatcaa
      241 caagcgggac ctcgccgcac aggccatcgc gagtgcgaac gaatccttca cctccggcag
      301 ttacccggcg gcacggggca aggccaacga cgggctcgcc ctcgcgaacc aggcctggaa
      361 tctctccctc gacctgaaga ccgagctcgg ccagggcttc cagctcccgg ggctcccgaa
      421 cctctcggcc ttcctccccg tcctcatcgt gatcgtcgtg gtggccatca tcgccggggt
      481 catcatctac cggaagaaga tgcactggga cgaactcggg taacaccccg cctgacggcg
      541 ggttgcccca gttcggtttc tcacgacttg gccgcgacta cccgtcgcgg cactcctatt
      601 tcaaacggaa atagtcgtcg tcgctcgagg tcggtttctt acgaaacctc cctcttacga
      661 aacctcccta gtgaagatat ccaggttcat actccccatt ttcccgtccc tgaggaagta
      721 ccacgccagg agccggtcct ggttatcgta ggaggaaaac cagtagttca gcctgatggt
      781 tctcttctcg tacccgtcga ggaatgagta gctcgactgg tcgtaggcga tgaccaggtc
      841 gtagttgttg tcatagatgg ttgcctcgtc caccctcttc ccgtagtagg cgagcttctt
      901 cgctatcacc ccgcggtagt accagggcag cggccagtag ttctcggagg ccacggcgac
      961 gcggctcgag ttgtcgatct gggaaaagag gccccggaga tcctcggagt tctgtacctg
     1021 gacgatgggc tcgttcacat cggcggggac aaaggaggtg tgccaggtgg cgaggacgag
     1081 gaaggcgacc gagacgatcg cgatgatggt ctttgtgtcg gtcagacggt acatggccac
     1141 gaagaccgcc gggaggagct gggggatgat gagccagggg accttctcgt tcacgatggc
     1201 gtagaaggcc atggagagga ccatccacca gatgcagaag ccgaagaact cctccctctt
     1261 tgcctccatc ggggaaaggg cattcggagc cgcccccaac cgggcgagga gggtctccgt
     1321 ggaaggctcc tcgcgccgga tcctccggcg gagatcctcc caccatccgc ggaggtcggt
     1381 gcgggccgcg agaaactgga gggtcccggc gatggcgagg ataaggatgg ggagctcgta
     1441 gatggcgaag aggaggaggt agaagtacca gggcccgcag agccggcatg cctcgtgtat
     1501 ctcgagccag tggccgatgg cccgctgggc ggcatcgagg aggatctccg gatgctgccc
     1561 gagggaggag tagaagaagg cgatgatccc gagggcgact accgtgaaga gggcgaggtc
     1621 aaccctccac cgggagggga accggatccg tcccctccag gcggcgtaga tgaagtagat
     1681 gccgaagatg aggaggatga ccgggaattc ctccttgcag gccatccccc cggcgaccgc
     1741 gactgccacg ccgaggccgt accaccggct gccggtctcg atgtaggcga gaagggtgac
     1801 gaggaggagc atgatgaaaa agagcatgaa gatgtcgtgc ctgaggaacc gggagaagta
     1861 cacgagatcc ggtgaaagag cgaggaagag ggcaccgacg agggtctggc gacggtccag
     1921 gtaccccatc cggtagaggg gatagacgag ggcgacgatg agggtcccga ggagggaggg
     1981 gatgagcctt cccacgacgt ccccgtcccc gaggagccag aacatgccgg cggtcgcgta
     2041 gtagaggagg gggccgtggt acgaggggtc gtaggtccag atcccctcgt ggaggagccg
     2101 gtaggagaac cagccgtgga tggcctcgtc atggtggaaa agcttgaggt ccagctggga
     2161 gaaacggaga agaacggtga gaacgaggat gaggaggaag atacggtcga aggtaaaaat
     2221 ggagcggagg cgctcggtga gccaggcggt ctcccgccgc atgcctagct ctccaggacg
     2281 atctcgatcc cgatatcctt cggcacctgg atgcgcatca gctgccggag cgcccgctca
     2341 tcggcatcga tgtcgatgag ccttttatgg atccgcatct gccaccggtc ccaggtcgcg
     2401 gttccctcac cgtcggggct cttcctgacg gggaccacga gcctcctcgt cgggaggggg
     2461 atcggcccgg ccagattcac gcccgtccgc tccgcgatct cccggatacg ctcgcagacc
     2521 atctcgacct ttttgcagtc cgttcctgtc aggcgtattc tggccttctg catgactttc
     2581 ccccgaaaaa attatctcat catcttcggc acgatgtcga tgcacatgcc cgcggcgatg
     2641 gtggagccca tgtcccggat ggcgaacctt cccatggggg ggatctcctt gatcttctcg
     2701 atgacgaggg gccggctcgg cttgatctgg acgatggccg cgtcgccggt ctttaagaag
     2761 gtggggttct cctccttcgt ctggcccgtc ctcgggtcga gctttttctt gagggcggtg
     2821 accgtgcagg ccacctgagc ggtgtgacag tggaacacgg gggtgtagcc cacggtgatg
     2881 gcgctcgggt ggtagaggat cacgatctgg gccgtgaact cgtcggcaac cgtcgggggg
     2941 ttgtcaacag ggcctaccac gtcgccccgc cggatctcgt tctttgcgac cccgcggatg
     3001 ttgaagccca cgttgtcccc gggcaccgcc tgggggatct cctcgtggtg catctccacg
     3061 gatttgacct cgcccgtctt gttggccggc atgaagatga cgttcatccc cttcttgagg
     3121 atgccggtct cgacccggcc cacgggcacc gtcccgatcc cgctgatgct gtacacgtcc
     3181 tggatgggca ggcggagagg cagggtcgtc ggcttctccg gctccttcag ggtatccagg
     3241 gcctcgagca cggtggggcc cttgtaccag ggggtcttgt ccgacttctt cgagatgttc
     3301 acgccctgga aggccgaggc ggggatgaag agcatgtcat cgggcttgta cgcgaccatc
     3361 ttgaggaggg ccgtgacgtc ggtcttcgta gcatcgaagc gcttctggtc atagttcacc
     3421 gcgtccatct tgttgatgac gatgatgagc tggttgatgc ccagggtacg ggccaggaag
     3481 acgtgctcct tggtctgttc ctgtacgccc tccgaggccg cgaccacgag gaccgccgcg
     3541 tccgcctggg aggcgccggt gatcatgttc ttgacgaagt cccggtgccc gggacaatcc
     3601 acgacggtga agtagtactt ggcggtgtcg aaccgcttgt gggcgatatc gatggtgatc
     3661 ccccgttccc gctcctcctt caggttgtcc atgacccagg cgaactcgaa ggttcccttc
     3721 cccttgctct cggcctcctt ccggtactgc tcgatgacat gggggggtac ggctcccgtc
     3781 tcgaagagga gcctccccac gagagtcgac ttcccgtggt cgatgtggcc gatgatggca
     3841 agattcagat ggggctttgc cgctgccatt ttccttgatc ctccagtgta tcgaatgaaa
     3901 tacgatgggc tcacaatccg tgtgatgcga gtgtaaatta ttggggacag gactatataa
     3961 attatagtca caggcccgtg ttttcctgcc ggagagaacc cggtctgggg catgccggcc
     4021 gctgtgtccg ttcgggggaa aactgcccga aggcatgaat tccgggagga tcgacatcct
     4081 catatcccat ggggcgtcca tgatcacagc gatgaaggtt ctctcctttt cccgcagccc
     4141 gctatccgag cgggtgacgg tccagtgtgc cgacggcgag gtgaccgtgc acagccactg
     4201 cgcgtactgc acgcactgtg cgggtgtcca gatcgggaag aggtccatgc cctccccgat
     4261 tctttcggcc tacaaggacg tgaggagggg gacggccccg gacgaggccc tcatgaacgc
     4321 gggaatgatg ttcaataccc tgatccggga cggggatgcc atcaactgcg acgatgacgg
     4381 gaacgagggg ttctcgtcca tgtaccatca ctgctgaagc tggccggcga tctcgttcct
     4441 gaacccctcg aagtgcaggt cgtccagctg gtcggaaagg agccgcccgc tccaggcccg
     4501 ccggtagacc cactggatga tccgctcgac ggcctccacc accttgtcct cctcctcgat
     4561 ggcgaggagg gtccgcccgg tccggggatg ccgccccctc ctgcctccca ccatgaccag
     4621 gtagtgggtg tgctctgact tcaggaggtg gaaggggcag gactggatgc agaccccgca
     4681 ctggacgcac ttctcctcgt caaggaccga gatcccgttc ctgatggaga tggcgcattc
     4741 cttgcagtac tcggcacagg tcccgcagcc cgtgcagagg ccggggatcc gcctcggctt
     4801 cacccggccg atgatcccga tctcgttcag catcgggctc gtgcaggcgt tcgggcagcc
     4861 ggagatggag atgcgcatct tcacgggcat ctccttcccg aagagccgct cgtcgagctt
     4921 cctcgcgagg ctgatggtgt cgaccatccc gtacttgcac ctctccttcc cgggacacgc
     4981 gacgatgttc accacctcgt ccttctcgga cccgaggggg gtcccgttct tcgcgagggc
     5041 ccgggtgatc cggaccaggt gcttcggatt cacgtggggg atttcggccg cctgccggcc
     5101 ggtgaagtgg acgaagccct tcccgtacct cttcgagatg ttcgcaaggc ccctcatctg
     5161 ctcgaccgtg aggttccccg ccgggaccct cacccggatg gtgcagaggt cgacgtcccg
     5221 ctcggtaatg acgccgcccc ggagatggat gccgtaatgg ggacccatgg tacaggagga
     5281 tgcaccccga tgagataaaa agggtgatct ttttctcagg ggatcaggac aagggacgcg
     5341 atcacgagag cccggactat ctcgtgggtc gcaccggcca ggtcaccgtt caccccgccg
     5401 aagaggtggt ccccgagggc gatcattgcg gcaacggtga tggccgtgac cgctcccgcg
     5461 aggaggaggg aaccggacgg gagaaggaga aggaggggca gggcggggac gggggcgtag
     5521 aggccgtgca cgggccgggc accgccgtgg agggtcgcgt ggatcccctc ccggaagggc
     5581 cggccggcca cggtgatcca ggcggtcccc aggcgggcaa ggacctcccc ggcgaggatg
     5641 gcgatccacg cggatgggac agactggagg gccgcgaagg atacgagggt caccacgagg
     5701 ccgaggccga tacccccggc cccgatggcc cggtcggtca gggccgcgac ccgtttctcc
     5761 cggctgccat gggccatgag gccgtcgccg aagtcgagga ggccgtccag gtggtggaag
     5821 ccggtgacga ggagggcgag ggagagcgcg atggccgccc ccagggggcg cggtgcgatg
     5881 aggaatgctg cgagggcggc gatccccccg gacacatagc ccgcgagagg gtacagccac
     5941 gggtggcgtg cgaaggcgcc gaggtcgacc gttctcccca gggggagacg ggtctcgaac
     6001 tggaggaggg cgatgaccgg gtccaggatc attccacgca ctccgtgtag agtctcgagg
     6061 tgcagccggc gatgagtccc gccacggcat cgtccaggaa cggccccagc cgggagagga
     6121 tcccgggttt cttctggtcg taccgggtga actcgaaccg ggcgcaggtc cccccgatgt
     6181 attcggcgat ggccatgccg aggatctcgt ccgagaggag aaagaccggg tcgcccccga
     6241 tggtcccggg cctccccgtg cagagttcct cctcgaggag gaccgcggcg aggaggaggg
     6301 aggagacgtt cgggtcccgc atggcctttt cgagcttccg ggcaacgacc cccccggcct
     6361 cctccgggga catcccgtgg gggacgtaga gggccatcgc ggcctccacc atcgagggga
     6421 ggtcgattcc ctttgcctcc agcctctccg cgatcccgaa catgatgtga tccatttttt
     6481 atccggggct tcttatagag atcggtatgc ccttcctctc gggtacgccc ccgttccgcc
     6541 cgaagtgtcc ggtctttgcc atcgtcctcg ggaacaccct tctctccacg gtccccgggg
     6601 tgtcgggggc cgggatgtcc cccgagaaga cggtgctcac gccgatcctc gactccgagc
     6661 tcatcacccg cggggtcatc cggagcagga ggtggaaacc gaacaccccg acgggctgtc
     6721 ccaccccggc ggtcatcacc cgggcgatga tggtgctcct cggcatggag ccggtcttcg
     6781 tgaatgccgg gctcacgacg aagcccgtgg tcccctgcct ggacgtgtac ggggaaccgg
     6841 ggcgggaccc ccgcgacggg gatgccgtgc cgatggcgag ggagctctac gcccggggtg
     6901 aacaggtcgg aaggctcctc tcgcgggcag gggacctcct gctgctcggc gagtgcgtgc
     6961 ccggcggcac gaccaccgcg ctctgcgtcc tccggggcct gggaatcccg gcgagcgtct
     7021 cctcctcctt tgtggacaac ccggtggccc tgaaggaggc gatctgcgcc cgggtccagg
     7081 agcgtatccg ggccgagaca ccgacggatc ccctcgatgt catccggatc ggcggggatc
     7141 cgatgctcgc ggtcgcggcc gggattgccg cctccttccg gggcgatacg gtgcttgcgg
     7201 gtgggaccca gatgctcgcg gtcgccgcgg tgctccggga tctcgggaaa aaggtgcccc
     7261 cgatcgtgac caccgtgtac gtccgggacg atccctcggc ggggttcgcc gccacggcag
     7321 ggatggtcgg ggcccaggtc tactacatcg atccggactt cggcggcatc gggcatccgg
     7381 gcctggcccg ctattgcgcc ggggaagtca aggaagggat cggggccggg ggcgccatgt
     7441 tcctcgcccg gatcatgggg aggagcgagg aggagatccg ggagacgatc ctctcgaccg
     7501 cctcgtcgta ttgaaaacct tctaaagatc cggaggccaa tgtacctccc atgcggcccc
     7561 tctacgtggt gaacaacttc gggcagttca accacctgat cctccgcagc ctccgggaca
     7621 tggggattcc cgccgagctc gtgccgaaca cgaccccgac cggcgaggtc gcgggggggt
     7681 gccgggggat cattctcggg ggcggcccct ccatggagag ggccgggaac tcggcccggt
     7741 acctggagct cgggctcccg gttctcggga tctgcctcgg gatgcaggcc atggccctcg
     7801 cccggggcgg gaaggtctca cccggatcca tgggcgggta cggcctcgtc gaggtggaga
     7861 tctctgaacc cgacgagatc ctcgcgggtt acccctcggc gacgagcgtc tgggcttccc
     7921 acatggacga ggtcacccgg atgcccgacg ggttcaccct cctcgcgagc tcccgcatct
     7981 gctccatcga ggccatggca aggccgtcgg agcacctgta cggggtccag tggcacccgg
     8041 aggtgagcca cacggtgaac gggcggctta ccttccagaa cttcgaccgc atctgcggcg
     8101 gggcctgaac cgttaccgga ggtggccatg aaggattctc cagggactcc ggatctcgcg
     8161 gacatcgcgg cggatatccg gaggatcggc ttttcttgcc tgaactgcgg ctcctgctgc
     8221 cgttcgggtc cggacgaccc gggcctcgtt tttgcctccc acgccgagat cgaggccctg
     8281 gtctcctcgg gcgggggagc atgggaggag gtcgcggttc cctatcctga attcatcccc
     8341 tgcggtgacg ggtctgccgt cacctttggc tggtgcctgc gccaggagga gggccggtgc
     8401 cggttcctct cggatggctc ctgtaggcgc taccgtgcac gtccctggat ctgccggacc
     8461 tatcccttcg ccctggtgga cggggaactc accgtctcgg actgccccgg tctcggtgaa
     8521 ctcgtttcag aggaagatgc cctcgccctc gcagaggctc tcctcgaacg ggcccggtgc
     8581 gaggcagatg atgaagaccg ggtaaggaag atcttcacct ccgagaggat cccgaagggg
     8641 aagcggtgcg tggtcgatgc atcagggatc accgtctgcc ccgattgatg ggaggctcgt
     8701 accggggtaa cgggttcccg cgcaagcctc atctacctcc gtgaccaaac cgccgggtat
     8761 gaatgttggt acgaccatca tcaaggggag gagatctatc cggagcttcc gggacaccga
     8821 gatcccggag gatgccatcc gggatgccct cgagtgcgcc cggcaggcgc cgaccgccat
     8881 gaacggccag ccctggctca tcggtgtggt ggaagagaag gagaccctgc agaagatcgc
     8941 gggtctcacc gattacggga agcacatcgc cggggcccgg gcctgcttcg cggtcttcgg
     9001 ggagaagaag gcgaaatact acctggagga ctgctgctgc gccacgatga acctgatcct
     9061 cggcctctgg gcctatggga tcgggtcctg ctgggtggcg ggggacaaga agccctacgc
     9121 gggtgccgtg cgggagctcc tcggggtccc cgactcctat accctcgtct ccctcgttcc
     9181 cgcggggtat ccagccgata tgacccttcc gccgaagaaa gaactctcag ccatcagctt
     9241 ctcggagcgg ttcgtcgccc ggtagcacgc tctgcttggc ggggcagaag gggcagatgg
     9301 aggtctcgac atcctcctcc ttttccctca ccgggcagta gtagaccccg tcacgcatct
     9361 cgacgatgaa cccgccgggg aacggggtgc cgacgggatg gcccgggagt tcccggacaa
     9421 agatggtgaa ggcggcgatg aggtagtaga agagaacgag ccggagatcc ccctcctccc
     9481 tgtcgggtag cccgaggagc atatcgcaga attcggccag ttttccctcc ggaggatcct
     9541 gaaggtcctc gaacctgccc ctccgatggg cgaggatgat ggcgtgatgg gtcccgaaca
     9601 ggtgatccat cattctcggc tggagccgtg accggtaatg gggagggacg tgccggaggt
     9661 cccgttccac gaaggccctg aggcggagga ggtcgtacag ggtgtaggtg gcgatctcat
     9721 cggcgacggc ggcggcgagg gcctccttcg attttgcctg cctcatcctt gccgagagga
     9781 ccgcgatccg ggttctcatc gattccttat ccaccggaag ctccctccac ctctatgcaa
     9841 gaagttggac aaccgctgaa ttgaaccttc cgcgcccacc gcccggatcc cctgagcgcc
     9901 acgctcgccc ggatcccttc tacgcccgcc cgcctgg
//
LOCUS       DATGDI010000202         4528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_370368, whole genome shotgun sequence.
ACCESSION   DATGDI010000202 DATGDI010000000
VERSION     DATGDI010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370368"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="VKO45_07550"
     CDS             complement(<1..1410)
                     /locus_tag="VKO45_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HMA05766.1"
                     /translation="MDLVIGTAGDRDFSIDAQHLVTGRTCIIAQSGAGKSWAIAVLCE
                     RLCAAGIGFCIVDTEGEYFSLRERYPVLWVGSDEGCDEDIERADLKDLMLRAVVNGTA
                     VVFDVSETDMREKVAALAEILYDIASAHRIPYLLILEEADKFIPQAKESLKKIEEISR
                     RGRKRGLGLLVATQRPSLVTKNVLSQCNNQAIGRLSIENDLRAVDLFFGSRAELEELA
                     GLAPGEFFVMGGFVRKKTRMRFAPRETVHPGTTPELSRRLGASPIPGKAGGRPPAARS
                     PPGGTGPVEPAQVPPLNRLPGPAPVISREEAVALAEKRRRRGAILGLGENRLVSVELV
                     AWPIARIRARYHGGLLGRTVREVSFLLDAVHGDQVRIRRGFSLRPGFSEVMGLSSGAV
                     RVLSRLPLAGGTPAEAEVLSGLPRDSVAAALSALQERRLITEAGRAGEAKILIPLLSR
                     RLPALSSLKGEGDLPPGQGS"
     gene            1605..1751
                     /locus_tag="VKO45_07555"
     CDS             1605..1751
                     /locus_tag="VKO45_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05767.1"
                     /translation="MAHHLYAIATTTKNGDLRREALLFLGALERGGSMDAAQALKKIK
                     DRNA"
     gene            complement(2044..2397)
                     /locus_tag="VKO45_07560"
     CDS             complement(2044..2397)
                     /locus_tag="VKO45_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5518 domain-containing protein"
                     /protein_id="HMA05768.1"
                     /translation="MSKQNFWLAAIIGAIVMFLLSFLPVIGPLIGGFVAGLIARGKPW
                     NGAKAGFVAGIIGAILAALVLFLGATILGGIFHHALAGLLIGAGIGVIVVAIGLYHAL
                     LGLVGGAIGAAIQKD"
     gene            2599..3045
                     /locus_tag="VKO45_07565"
     CDS             2599..3045
                     /locus_tag="VKO45_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA05769.1"
                     /translation="MEEEPDVEVRVVTAWDTEEILSLYRSAGWWKEGRDDPSEIPGII
                     RGSLVFVIATEGETGRAVGMGRAISDGVSDAYIQDLAVLPAYRGRGIGRAILRRILGA
                     IRERGIEWIALIAEGGTGDFYRGLGFEVEEGDIPMMYRGEEKDVLR"
     gene            3032..3988
                     /locus_tag="VKO45_07570"
     CDS             3032..3988
                     /locus_tag="VKO45_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HMA05770.1"
                     /translation="MFSAEDFSPVTLEDRDLFSSFYRSYPQLHSDNSFANMVCWNHYA
                     HYRKAVTGDHLVLSSTVNGETRFRFPIGPRDPEVTKEVLRLSLDAGGSTPFVTFNGED
                     RSYLGEVCPELVLHPFRDFFEYVYLSSDLADLPGKPYLKIRHQLNRFHRRCSFATEAI
                     TGENTGEAREFLERWCDWRDCETDPVLAKEREAILFALAHLEVLNLSGLLIRVRDQEG
                     ATDAGRIGAQDGNRIGAMAIFQELNPETAVVHFEKGLPDCEGIYRAVNQETALLLRDR
                     YPFINRESDLGVPGLREAKERYHPHHLVGVYYARRSEMERVI"
     gene            complement(3981..>4528)
                     /locus_tag="VKO45_07575"
     CDS             complement(3981..>4528)
                     /locus_tag="VKO45_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMA05771.1"
                     /translation="FVGGLFLGKDHALIIRPPQGYTASLATPTPDSTRDGLTWYGLRS
                     LGAGEPRVVFSRDSFPLTPALFGIGLAVLALGIYAVRRRFRRAPTVPAVEQVPAPVDP
                     LAALELGDRILALLAGRGGSLFQSEIVEILGLPKSTVSEAINRLHDGGKIEKVRKGRE
                     NLIRIPKGDEGDGSADGPASR"
BASE COUNT          806 a         1448 c         1556 g          718 t
ORIGIN      
        1 ggatccctgg ccggggggaa ggtcgccctc gcccttcagg gaagagagtg ccgggagcct
       61 ccgggagagg agggggatga ggatcttcgc ctcgccggcc ctgcctgcct cggtgatgag
      121 cctccgctcc tggagggccg agagggcggc ggccaccgaa tcccggggaa ggccggagag
      181 cacctcggcc tcggccggcg tcccaccggc gaggggaagg cgggagagga cccggaccgc
      241 gccggaggag aggcccatca cctcggagaa gcccggccgg agggaaaatc cccggcggat
      301 ccggacctgg tccccgtgga ccgcatcgag gaggaaagac acctcccgga cggtcctccc
      361 gagaagcccc ccgtggtacc tcgcccggat ccttgcgata ggccatgcca cgagttccac
      421 ggagacgagc cggttctccc cgaggccgag gatcgccccc cgcctccgcc gcttctcggc
      481 gagtgccacg gcctcctccc gtgagatgac aggggccggc ccgggaaggc ggttcagagg
      541 gggtacctga gcgggctcga cgggcccggt accgccgggc ggggatctcg cggccggggg
      601 acggcccccc gccttccccg gtatcgggga ggcccccagg cgccgggaaa gctccggggt
      661 cgtccccggg tgcacggtct cccggggcgc aaaccgcatc cgggtcttct tcctcacgaa
      721 tcctcccatc acgaagaact caccgggggc aaggccggcg agctcctcga gctcggcccg
      781 ggagccgaag aagaggtcca cggcccggag gtcgttctcg atggagagcc tcccgatggc
      841 ctggttgttg cactgggaga ggacgttctt cgtcacgagg gagggccgct gggtcgcgac
      901 gaggaggccg aggccccgct tccgccctct cctcgaaatc tcctcgatct tcttgaggga
      961 ctcctttgcc tgggggatga acttgtccgc ctcctcgagg atgaggaggt aggggatccg
     1021 gtgggcggag gcgatgtcgt agaggatctc cgcgagggcc gccaccttct cccgcatgtc
     1081 cgtctcggaa acgtcgaaga cgaccgcggt cccgttcacc acggcccgga gcatcaggtc
     1141 cttgaggtcg gcccgctcga tgtcctcgtc gcatccctcg tccgacccga cccagaggac
     1201 cgggtaccgc tcccggaggg agaagtattc cccctcggtg tcgacgatgc agaagccgat
     1261 gccggcggcg cagagcctct cgcagaggac ggcgatggcc cagctctttc cggcccccga
     1321 ctgggcgatg atgcaggtgc ggcccgtgac caggtgctgg gcgtcgatgg agaagtcccg
     1381 gtccccggcc gtcccgatga cgagatccac aagaaaccgg tgaggacagg gggcattaat
     1441 ctatccccgg ctccccgccg cgacgggaca cgggaaggat ccgccatttt tctccctttg
     1501 ccgaacgctt cgccgtgcaa aggttgatga ggggacgtga acacctctcc gggattgagg
     1561 ggggcaagaa ggtggatacc cggagaaagg aaggaaacga tcggatggca caccatctct
     1621 acgccattgc gaccaccacg aagaacgggg acctgcgacg ggaggcgctt ctcttcctcg
     1681 gtgctcttga gcggggaggg agcatggatg ccgcgcaggc attgaagaag atcaaggatc
     1741 gaaatgcgtg agacaggtcc atcgaggaga ggtcctgtga tctattccct gaatggatga
     1801 tccggcccgt taccggtact cttacccggc ggatgccggt ccttgttccc tcgaatcgaa
     1861 catcatccgg agccagagct cggcgcagac gatccgcatt cccgcgaacc cgcacatcgg
     1921 ataagagtga tatcccgctt tctattcatc atggccccgg gaacgaggga cactgccccc
     1981 ggggtccttc attggaatgg cgacgtaggg cccatccggg gattttttaa aaaaaaagga
     2041 tggtcagtcc ttctgtatgg cagccccgat ggcgccaccc accagaccca gaagagcgtg
     2101 gtagaggccg atggcgacga cgatcacccc gatgccggca ccgataagga ggcccgcgag
     2161 ggcgtggtgg aagatgccgc ccaggatcgt ggctcccagg aagaggacga gggcggcgag
     2221 aatcgccccg atgatgcctg caacaaaccc tgccttcgcc ccgttccagg gcttcccccg
     2281 tgcaatgagg ccggcgacga acccgccgat gaggggtccg atgaccggga ggaaggagag
     2341 gaggaacatc acgatggccc cgatgatggc cgcgagccag aagttctgct tgctcatgat
     2401 cgcacctccc ggtactcgga aaggggaggg ataaatgtgg cgggttccct cccgttacct
     2461 gctccgatac cttcctgtag gccgggggcc gtcccttccc cccatgggta tatgtgaccc
     2521 ttcctcccat gtaccggagg aaaaccggtc ccggggagag aacccgggac ccccggtgga
     2581 accggaaggg gcattcgcgt ggaggaggag cccgacgttg aggtgagggt ggtcacggca
     2641 tgggatacgg aggagatcct ttccctctac cggtcggcgg ggtggtggaa ggagggaaga
     2701 gacgacccct ccgagatccc ggggatcatc cggggctccc ttgtcttcgt gatcgctacc
     2761 gagggagaga ccgggagggc ggtcgggatg gggagggcca tctcggacgg ggtttcggac
     2821 gcctacatcc aggatctcgc cgtcctcccg gcgtatcggg gccgcgggat cgggagggcc
     2881 atccttcgga ggatcctcgg ggccatccgt gagcgcggga tcgaatggat cgccctcatc
     2941 gccgaggggg ggaccgggga tttctaccgg gggctcggct tcgaggtgga ggagggggac
     3001 atccccatga tgtaccgggg agaggagaaa gatgttctcc gctgaggact tctccccggt
     3061 gaccctggag gaccgggatc tcttctcttc cttctaccgg tcttaccccc agctccacag
     3121 cgacaacagt ttcgcgaaca tggtctgctg gaaccactat gcccattacc gcaaggcggt
     3181 gaccggggat catctcgtcc tctcgagcac ggtgaacggg gagacccggt tccgcttccc
     3241 catcggcccc cgggacccgg aggtcacgaa ggaggtgctc cggctctccc ttgatgcggg
     3301 gggaagcacc cccttcgtca ccttcaatgg cgaggaccgg agctacctcg gggaggtctg
     3361 cccggagctc gtcctccatc ccttccggga ctttttcgag tacgtgtacc tgtcctcgga
     3421 cctcgcggac ctgcccggga aaccgtacct gaagatccgg caccagctga accggtttca
     3481 ccgccggtgc tccttcgcca ccgaggccat caccggggag aacaccggtg aggcgaggga
     3541 gttcctcgag cgctggtgcg actggaggga ctgcgagacc gacccggtgc ttgcgaagga
     3601 gagggaggcc atcctcttcg ccctggccca cctggaagtc ctgaacctct ccggcctcct
     3661 catccgggtc cgggaccagg agggggccac ggacgcgggc cggatcgggg ctcaggacgg
     3721 taaccggatc ggggcgatgg ccatcttcca ggagctgaac cccgagacgg cggtggtgca
     3781 cttcgagaag gggctccccg actgcgaggg gatctaccgg gccgtcaacc aggagacggc
     3841 cctcctcctc cgggatcgct atcccttcat caaccgggag agcgacctcg gggtgcccgg
     3901 cctccgggag gcaaaggagc ggtaccatcc ccaccacctg gtaggggtgt actatgcccg
     3961 gagatcggag atggagaggg tcatctagaa gcggggccgt cggctgatcc gtccccttca
     4021 tctcccttcg ggatccggat caggttctcc cgccccttcc tgaccttctc gattttcccg
     4081 ccgtcgtgga gccggttgat ggcctcgctc accgtcgact tggggaggcc gaggatctcg
     4141 acgatctccg actgaaagag ggagcccccc ctcccggcga gaagcgcgag gatccggtca
     4201 ccaagctcga gggcggccag cggatccacg ggggcaggca cctgctcgac tgccggtacc
     4261 gtcggagcac ggcggaacct cctcctaacg gcatagatcc cgagggcaag gaccgcgagg
     4321 ccgatgccga aaagggccgg tgtcaggggg aacgagtccc tgctgaagac gaccctcggt
     4381 tcacccgccc ccagggaccg gagcccgtac caggtgaggc cgtccctcgt gctgtcgggc
     4441 gtgggggtcg cgagggaggc cgtatacccc tgcggggggc ggatgatgag ggcatggtcc
     4501 ttccccagga acaggccgcc gacgaaga
//
LOCUS       DATGDI010000203        13669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_21938, whole genome shotgun sequence.
ACCESSION   DATGDI010000203 DATGDI010000000
VERSION     DATGDI010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 13669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13669
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21938"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="VKO45_07580"
     CDS             complement(<1..1287)
                     /locus_tag="VKO45_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HMA05772.1"
                     /translation="MEEGNRVTRIGLVQMKVSDDPEKNLERALAAAGEAARRGARIIC
                     LPELYRTRYFPQDECAGLQERSEKIPGVSARAFSAFAREHGVVVIVPVFERGESGAFH
                     NTAVVVDRDGGSLPLYRKVHVPHDPLYYERHYFVPGDGYRVYDTAGARFSVLICYDQW
                     FPEAARAATLMGAEILFYPTAIGRIRGAVPPEGDWQEAWETIQRGHAIANGIHVACVN
                     RTGEEGDLLFWGGSFVADAFGKVVARAGEGEEVLVTDLDLSMNRRVRDAWGFFRNRRP
                     DTYRILTDPVAREYTGPSGAWATPKSLGFSMPPEWEPHEATWLAWPHNPETFGDIPAV
                     QTAYLAIIGALAPGERVNLLVRDREMEVRVRGLLEGAGIDSARVSLIPFDYADVWFRD
                     FGPSFVVNRKDRTTAMVAWEFNAWGGKYPWIVRDTRV"
     gene            complement(1316..1900)
                     /locus_tag="VKO45_07585"
     CDS             complement(1316..1900)
                     /locus_tag="VKO45_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05773.1"
                     /translation="MILIEDLSYRPGSLSADEFVAPVERIVRTDGETTLIRHYADISG
                     PDLASADGVILCGTALADFGYLDGMEHFAWLPSFPRPVFGICAGMQVIAKTFGGEVRP
                     GVAIGMAGQEVTAPDPLLAGKDRFDAYELHSLSVVPPASFRVLAVSPSGIQVIRHRER
                     PVYGVMFHPEVRNEWLIRRFLSLCRLREGRDALM"
     gene            complement(1897..2577)
                     /locus_tag="VKO45_07590"
     CDS             complement(1897..2577)
                     /locus_tag="VKO45_07590"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017178 - diphthine-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HMA05774.1"
                     /translation="MRLGVLFSGGKDSAFAAWRAMQKEEVACMITVVPSNPESYLFHT
                     PNIHLTGLQAEAAGLPLVSMPSRGIEEEEVADLSRAVALAAREQGIEGVVTGAIHSVY
                     QATRVQRVCHDLGLWCFNPLWQRDEEEHLEEVVSSGFRFLVSGVSAAPLGAEWLGREV
                     DRPALDELKALARRHRMNPAGEGGEYETFVLDAPFFRKRIEILGSSREFHGDRGTFGI
                     TDARLVAK"
     gene            complement(2688..3860)
                     /gene="chrA"
                     /locus_tag="VKO45_07595"
     CDS             complement(2688..3860)
                     /gene="chrA"
                     /locus_tag="VKO45_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HMA05775.1"
                     /translation="MDESRPLKEPPSPARLFLSFLLLGATAFGGPAMVEYIRRLSVEE
                     KGWLDERSFRDGVALCTTVPGATAMQVAAYVGLRVRGVLGAAAAYIAFGLPAFLFMMV
                     LSALYLEMKGIGMATSAFQGLKLLVVAIVGAAALGFSRDTLTGIRPLAIALLAAALFL
                     AGVSPIAIILLAGVLGILLGTALPAGEGGSSGEAGGPTLRVVILIILGAAALVAALAL
                     LAPPLAEIFSLMLRIDLFAFGGGQASLPLLFHEVVTARGWLDAPTFLDGIALGQVTPG
                     PIVISATFIGYLLQGPAGGLVATIGIFLPSFLMVIGIAPYFPRIRDSPALSRAFGGIV
                     ASFAGLLISVTILFGSQIPWNLPRVLIGVGAFLALSGKVKVYWVVIAGVILSLLLL"
     gene            complement(3953..6499)
                     /locus_tag="VKO45_07600"
     CDS             complement(3953..6499)
                     /locus_tag="VKO45_07600"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HMA05776.1"
                     /translation="MPALNFSDFSGLSADEAHRRLLEEGYNEIPSAERKNLLAIVGEV
                     IREPMFLLLVGAGLIYFILGDVEEGAILLSFVLVIIGITAYQEQKTEKALEALRNLSS
                     PRALVIRNGSLQRIAGREVVRKDVMVVSEGDRVAADALLLSENNLLVDESLLTGESVP
                     VRKAVAAKTPADRRPGGDDQPWLYAGTLVVQGQGISEVMDTGMGTEMGKIGKALQSVQ
                     EEETRLHLETSRIVRNIALTGLLLCTTVIVVYGITRADWLHGFLAGITLAMAILPEEF
                     PVVLTIFLALGAWRLSRQNVLTRRIPAIETLGAATVLCVDKTGTLTENRMSVARLGVA
                     GESFPCSPRGRDQVPEPFHELLEFAILSSKKDPFDPMEQALLRKGGDDLRGTEHLHGD
                     WDLLQEYPLSRELLAMSNVWRSREGREYVIAAKGAPEAIADLCHLGAERQGEISAEVE
                     KMASDGLRVLGVAKAYFTEKDLPDGQHDFPFTFLGLVGFADPVRKDVPGAVAECGTAG
                     IRVVMITGDYPATARNIGRQIHLPLEDGLVTGPELDRADDLAIAGKIRGASIFARVVP
                     EQKLRIVEAFKASGEVVAMTGDGVNDAPALKAAHIGIAMGGRGSDVAREASSLVLLDD
                     NFASIVAAVRMGRRIYANLKKAMAYIISVHVPIAGISLAPVLLGWPLILLPVHIVFLE
                     LIIDPACSVVFEAEREEPDIMKRPPRRPEDRLLDRRTIILALAQGFVVLGFVLAIYLS
                     AVVRGLADDEVRTLTFVTIVMANLALIAVNRSWSENIVQTLRVPNRAFWAVIGGTLFF
                     LALAVAVPAARGVFRFAVFHPLDLLFCIIAGILSVLWFEVYKIARPSAGP"
     gene            complement(6551..7222)
                     /locus_tag="VKO45_07605"
     CDS             complement(6551..7222)
                     /locus_tag="VKO45_07605"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HMA05777.1"
                     /translation="MHPRWNLAIISGILILTFALSVAAYPYLPDRIVTHWGLYGEPNG
                     SMPKAWGLFFVPLISAGLAILLLAIPRIDPLRENIRKFRSDYEHFIAVLLLFLLYLQI
                     LVILWNTGARFNFSQVLSPAFGVLFYTIGILTGRARRNWFIGIRTPWTLSSERVWDRT
                     HALGGKLFRIAGILCLAGAAFPALAFFFILGPVLVISVWLVVYSYREYRKEEEAGTGP
                     AGPGA"
     gene            complement(7298..10171)
                     /locus_tag="VKO45_07610"
     CDS             complement(7298..10171)
                     /locus_tag="VKO45_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMA05778.1"
                     /translation="MGDAVGPGHGDEGGKGNRIFRYYPEDFGELRVKVLHMDLTFDVH
                     DDHTRVISYLTARTLDRTLQTLELNAKDLEIVSITSEGRRVTADYRRAESKIRLTFQP
                     RLTPGTEFTVRTETVCRPTRNILEGLYYDETPPGAPPTQITQCQQWGFQRLVPCIDDM
                     TAKCTYLTTIIADSRYTHLISNGDIAEARHQVEPGRDRIRYRNMVTPMAPYLFFLGVG
                     TYATFERQVEYPDGKTFMLELLVPPDSDPSIAREALGILHDAVIWVYLFTGPEQYRNL
                     VIRKEMYRLIREREALKTAGGDPRRIGDIRGLLKTYVSRITPGYQYTGTVYREIGMQN
                     SDFGGMENVGNTTITTNRIMPFPQMTDSAYEYLFRVKVHEYYHNLNGSEVTGRSPFEL
                     WLNEAVTVFVENQNHDFLFGPDYNRLGTALTLLTPDGGTLALDTGAMAMPIEPDGFND
                     PNDLITAVTYVKAPEFVRMVETLLGKEAFAGGLHLYHTRYRHGNASRADWVKCMEETG
                     GGDLAGFARAWLKETGYPVVTAEGSYDGEARSFTLTLQVSPSKEGKAWEFPFTYALLS
                     SDGKVLAEGMRRIAGTREEIAIRGVDRPAYASLNRGYSFYGRVKYDPGENALHLQART
                     DPDAVNRFIAFHGLTDREMARLLADPAATPSERFTSLFMDLLADTGWLERAGGQGLTI
                     LESVEDEGSAHRYRALFEARQRILSAIAARHRDAALALYREYAGDPVPAAVPDRIRAV
                     KRRQVKNLLLEILSTLDAPEVHALLLRQFRESHSASDRLNAFRLLLASSSPDRLPVFE
                     DFLTESSGNLVAWEAFLSTLAGSNAPDLVELVRRAETAPAFRIEQANDQRALYVRFAM
                     NRKVSLQMADGRALLLSILEKLARVNETSAVRALQPLAHLDRMEEEYYLPLAGILVDL
                     LGTFDPVGSPVVHNTVRRLLLGAPRALAAYETKFGRVRALHPE"
     gene            complement(10249..11481)
                     /gene="mmp10"
                     /locus_tag="VKO45_07615"
     CDS             complement(10249..11481)
                     /gene="mmp10"
                     /locus_tag="VKO45_07615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833686.1"
                     /note="Mmp10 (methanogenesis marker protein 10) is a
                     cobalamin-requiring radical SAM methyltransferase that
                     creates the methylarginine modification to methyl coenzyme
                     M reductase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase-arginine
                     methyltransferase Mmp10"
                     /protein_id="HMA05779.1"
                     /translation="MPQLTVDIGGRPGVDCRGYCDYCYFKHAKDVPPFGCRHCPPFRK
                     GCDYCSRSVKERYTGFRDLPAIAEDVLANLQMMEGDLTRITISGGGDPSCYPRFRDLV
                     ELIAGMEAPLHIGYTSGKGFDDRSLARFLLDHGLAEVSFTIFASSPKLRRKWMHDPTP
                     EVSLGILEDLCGGCDVYGAAVVIPGVNDGEVLGETCAWLEERGAKGLILMRFANTRES
                     GLILGNEPVLKGREMQTVEEFRDMVTGLAKEFRMKITGTPLWDPEIGSPFAILSEDGL
                     LDRLPRVTGRATVISGRVAAPFVQEILDRRGYRSRVIAAQKDIACLITIDDLERIDLS
                     GLEDVVIFPGRAFVHDEEAREVLTADGREREVLRGPETLTADGETSMGMTRTQVLEME
                     LEGLGNLIRLINQYGRRD"
     gene            complement(11544..11945)
                     /locus_tag="VKO45_07620"
     CDS             complement(11544..11945)
                     /locus_tag="VKO45_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05780.1"
                     /translation="MTGIVALVLMVAVILTGAVQKGRIKAIRVHHIFRFHRMLSIWFS
                     AFVVGTFVLGLLTTRGHGEPVLESVHGLLGLVVAILALAQLVPSLVIARRTRIRLPHR
                     VIGYLIVPLLVLQTVIGLGEAGLIPLIIPIR"
     gene            12260..12517
                     /locus_tag="VKO45_07625"
     CDS             12260..12517
                     /locus_tag="VKO45_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05781.1"
                     /translation="MKLNVKALSIASGIVIGLASLFVGEAAVFTGIGLDYVNIAGPLH
                     PGYSPTPLGALIMAIWMFIYGLIGGALIAAIYNRFAGPDMK"
     gene            12559..12741
                     /locus_tag="VKO45_07630"
     CDS             12559..12741
                     /locus_tag="VKO45_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HMA05782.1"
                     /translation="MAIDPVCKMEVDENRAKYGTRYKGKSYYFCTEGCKKKFEEDPEK
                     YSRMVTEIGDSGGKCH"
     gene            12753..13094
                     /locus_tag="VKO45_07635"
     CDS             12753..13094
                     /locus_tag="VKO45_07635"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HMA05783.1"
                     /translation="MDGFWYGMMDGYPYPFYGMFIWMGIVWVLQLVVGYFVFRDARER
                     GMSPVLWFILVVLPMIGWLFLVIYVVIRETGRPASSGEKSSAGGILDERFAKGEITAE
                     DYRKMKEELNR"
     gene            13108..>13669
                     /locus_tag="VKO45_07640"
     CDS             13108..>13669
                     /locus_tag="VKO45_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HMA05784.1"
                     /translation="MEGAHGSHTDAHAGHGVGGEEHHPVTGPGAPGGHRPGMHGAHRH
                     MLEDFRRRFIVSMILTVPILILSRSFQSFFGVSFSFPGSDLILLILSTAVYAYGGFPF
                     LKGIMEELRTRLPGMMTLIAVAITVAYGFSAATVLGLVTGEAFFWELATLIDIMLLGH
                     WIEMRSVLGASRALEELVRIMPSTAHV"
BASE COUNT         2446 a         4318 c         4618 g         2287 t
ORIGIN      
        1 gacccgggtg tccctgacaa tccacgggta tttcccgccc caggcgttga actcccaggc
       61 caccatagcc gtcgtccggt ccttccggtt cacgacgaag gaggggccga agtcccggaa
      121 ccagacatcc gcatagtcga aaggtatcag gctcacccgt gcggagtcga tcccggcccc
      181 ttccaggagc ccccgtaccc gtacctccat ctcccgatcc cggacgagga gattcacccg
      241 ctctcccggg gcgagggcgc cgatgatggc gaggtaagcc gtctgcacgg cggggatgtc
      301 accaaaggtc tcggggttgt ggggccaggc gagccaggtt gcctcgtggg gctcccactc
      361 ggggggcatg gagaatccaa gactcttcgg ggtggcccac gcgcccgatg gccctgtgta
      421 ctcccgggcc acgggatccg tgaggatccg gtacgtgtcc ggccggcggt tcctgaagaa
      481 cccccatgca tccctcaccc tccggttcat ggagaggtcg aggtcagtga cgaggacctc
      541 ctccccctca ccggccctcg cgacgacttt cccgaaggca tccgcgacaa aggacccgcc
      601 ccagaagagg agatccccct cctcacccgt gcggttgacg caggccacgt ggatgccgtt
      661 cgcgatggca tggccccgct ggatcgtctc ccaggcctcc tgccagtccc cctcgggggg
      721 caccgccccg cggatccggc cgatagccgt ggggtagaag aggatctcgg cgcccatgag
      781 ggttgcggcc ctcgccgcct cggggaacca ctggtcatag cagatgagga cggaaaaccg
      841 tgccccggcg gtatcgtaca cccggtaccc atcgcccggc acgaagtagt gcctctcgta
      901 gtagagggga tcatggggca cgtggacctt ccggtataac gggaggctgc ccccatcccg
      961 atcgacgacc accgcggtgt tgtggaaggc ccccgattcg ccccgttcga agaccgggac
     1021 gatcaccacc accccgtgct cccgggcgaa ggcggaaaag gcccgggccg atactcccgg
     1081 gatcttctcc gagcgctcct gcagtccggc gcattcgtcc tgcgggaagt agcgggtccg
     1141 gtacagctcg gggagacaga tgatccgggc accacgccgg gccgcctccc cggcggccgc
     1201 gagggcccgc tccaggtttt tctccgggtc atccgagacc ttcatctgga cgaggccgat
     1261 cctcgtgacc cggttccctt cctccatgat tcctccacca gcgtgtgcct cgcacctaca
     1321 tgagggcatc cctgccttcg cgaaggcggc agagggagag gaacctccgg atcagccact
     1381 cgttcctcac ctcgggatga aacatcaccc cgtacacggg cctttcccgg tgccggatga
     1441 cctggattcc tgacggggag acggcaagga cccggaagga ggccgggggt accaccgaga
     1501 ggctgtggag ctcgtaggcg tcgaaccggt ccttcccggc gaggagggga tcgggggcgg
     1561 tcacctcctg cccggccatc ccgatggcga cccccggccg aacctccccc ccgaaggtct
     1621 tcgcgatgac ctgcattccg gcacagattc cgaatacggg ccgggggaag gaggggagcc
     1681 aggcgaagtg ctccatcccg tcgagatagc cgaagtctgc aagggcggtc ccgcagagga
     1741 tgaccccatc ggcggatgcc agatcgggac cggagatatc ggcgtagtgg cggataaggg
     1801 tggtttctcc atcggttcgt acgatgcgct ccacgggggc cacgaactcg tcggcggaga
     1861 gggagccggg ccggtacgag aggtcttcga tgaggatcat ttcgccacca gccgggcatc
     1921 cgtgatcccg aaggtcccgc gatccccgtg gaactccctg gaggaaccca ggatctcgat
     1981 cctcttcctg aagaacggcg catcgaggac aaaagtctcg tactcgcctc cctcccccgc
     2041 cgggttcatc cggtgccggc gtgccagggc cttcagctcg tcgagggcag gccggtccac
     2101 ctcccgcccg agccattcgg cgccaagagg tgccgccgag acccccgaga cgaggaaacg
     2161 gaaacccgag gagaccacct cctccaggtg ctcctcctcg tccctctgcc agagcggatt
     2221 gaagcaccag aggcccaggt catgacagac cctctggacc cgggtggcct ggtacaccga
     2281 gtggatcgcc cctgtcacca ccccttcgat cccctgctcc ctggcggcga gggcaacggc
     2341 ccgggagagg tcggcgacct cctcctcctc gatcccccgg ctcggcatcg agacgagggg
     2401 gaggcccgcc gcctcggcct ggaggccggt gaggtggatg ttcggggtat ggaacagata
     2461 gctttcaggg ttcgagggaa cgacggtgat catgcaggcg acctcctcct tctgcatcgc
     2521 ccgccatgcg gcgaaggccg agtccttgcc gcccgagaag aggaccccga gcctcatgtc
     2581 tcccggcaag ctgggggatg gcctgtaaaa agcctatccc cgcccttaga tctgcgcccc
     2641 ccgcccttag atccgcgcct ctccgccctt agacccgcgc ccccccttta caggaggaga
     2701 agggagagga tgacccccgc gatgaccacc cagtacacct tcaccttccc cgagagggca
     2761 aggaaggcgc ccaccccgat gagcacccgg gggaggttcc aggggatctg ggagccgaag
     2821 aggatggtca ccgagatgag gaggccggcg aaggaggcga cgataccccc gaaggcccgg
     2881 ctcagggcag gcgaatcccg gatcctcggg aaatacgggg cgatgccgat gaccatgagg
     2941 aaggacggga ggaagatccc gatggtcgcg accaacccgc cggcagggcc ctgcaggagg
     3001 tagccgatga aggtcgccga gatgacgatg gggcccgggg tgacctggcc gagggcgatc
     3061 ccgtcgagga aggtgggggc gtcgagccag ccccgggcgg tgaccacctc gtggaagagg
     3121 agggggaggg aggcctgccc ccctccgaaa gcgaagaggt cgatccggag catcagggaa
     3181 aagatctcgg caaggggcgg ggcgaggagg gcgagggcgg cgacgagggc ggccgcgccg
     3241 agaatgataa ggatgaccac ccggagcgtc ggccctcccg cctctccgga cgagccccct
     3301 tcccctgcag ggagagccgt tcccaggagg atcccgagga ctccggcgag gaggatgatg
     3361 gcaatggggc tcacgccggc gaggaaaagg gccgcggcga gaagggcgat ggcgaggggc
     3421 cggatcccgg tgagggtatc ccgggagaag ccgagggcgg ccgcgcccac gatggcgacc
     3481 accaggagct tgagcccctg gaaggcggag gtggccatcc cgatcccctt catctccagg
     3541 tagagggccg agaggaccat cataaagagg aaggccggga ggccaaaggc gatgtaggcg
     3601 gcggccgccc cgaggactcc ccggacccgg agcccgacgt aggccgcgac ctgcatcgcg
     3661 gtggccccgg ggaccgtggt gcagagggca accccgtccc ggaaggagcg ctcgtccagc
     3721 caccccttct cctcgacgga aaggcgccgg atgtactcca ccatggccgg gccgccgaag
     3781 gccgttgcac cgaggaggag gaaggagagg aagagccggg cgggggaggg gggttccttc
     3841 aggggccggc tctcgtccat cggatcctct gctcttccat gggttgacat ccctgataaa
     3901 tcaagggaaa tacccctgcc ggccgcggcc cggcagggcg gcccgggcga tctcagggcc
     3961 cggccgacgg ccgtgcgatc ttgtagacct cgaaccagag caccgacagg atcccggcga
     4021 tgatgcagaa gagaagatcc agggggtgga agacggcgaa ccggaagact ccccgggctg
     4081 cgggaacggc gaccgccaac gcgaggaaga agagggtccc cccgatgacc gcccagaagg
     4141 cccggttcgg gacccggagg gtctgcacga tgttctcgct ccaggaccgg ttcacggcga
     4201 tgagggcgag gttggccatg acgatggtga cgaaggtgag agtccggacc tcgtcatccg
     4261 cgagaccccg gactacagcc gagaggtata tggcgaggac gaacccgagg accacgaacc
     4321 cctgtgcgag ggcgaggatg atggtcctcc ggtccaggag ccggtcctcc ggcctccggg
     4381 ggggcctctt catgatatcg ggctcctccc gctctgcctc gaagaccacc gagcaggcgg
     4441 ggtcgatgat gagctcgagg aagacgatgt ggacggggag gaggatgagg ggccagccca
     4501 ggaggacggg ggcgagggag atccccgcga tggggacatg gacggagatg atgtaggcca
     4561 tggccttctt caggttcgcg tagatccgcc tccccatcct cacggcggcg acgatggagg
     4621 cgaagttgtc gtccaggagg acgagggagg acgcctcccg ggccacgtca gacccccgcc
     4681 cgcccatggc gatcccgatg tgggccgcct tgagagcggg ggcatcgttc accccgtccc
     4741 cggtcatggc caccacctcc ccgctcgcct tgaaggcctc cacgatccgg agcttctgct
     4801 cgggaacgac ccgggcgaat atgctcgccc cccggatctt ccccgcgatg gccaggtcgt
     4861 ccgcccggtc gagctcgggg ccggtcacga ggccgtcctc cagggggagg tgtatctgcc
     4921 tcccgatgtt cctcgccgtg gccgggtagt ccccggtgat catgacgacc cggatgcccg
     4981 cggtcccgca ctcggcgacg gctcccggca cgtccttcct caccgggtcc gcaaagccca
     5041 cgaggccgag gaaggtgaag gggaagtcat gctgcccgtc cgggagatcc ttctcggtga
     5101 agtaggcctt cgccaccccg aggacccgga gcccgtccga ggccatcttc tccacctcgg
     5161 ccgagatctc cccctgccgt tccgccccca ggtggcagag gtcggcgatg gcctcggggg
     5221 cgcccttcgc cgcgatgacg tactcccggc cctcccgcga gcgccagacg ttggacatgg
     5281 cgaggagctc ccgggagagg gggtactcct ggaggagatc ccagtcgccg tgaagatgct
     5341 cggtcccccg gaggtcgtcc ccgcccttcc tgaggagggc ctgctccatg gggtcgaagg
     5401 ggtccttctt cgaggagagg atggcgaact ccaggagctc gtggaagggc tcggggacct
     5461 ggtcccggcc ccgggggctg caggggaagg actccccggc cacccccagg cgggccacgc
     5521 tcatccggtt ctcggtgagg gtgcccgtct tgtccacgca gaggaccgtg gccgccccca
     5581 gcgtctcgat ggccgggatg cgccgggtga ggacgttctg ccgggagagc cgccaggctc
     5641 ccagggcgag gaagatggta aggaccaccg ggaactcctc ggggaggatg gccatggcga
     5701 gggtgatccc ggcgaggaag ccgtggagcc agtccgcccg ggtgatcccg tagaccacga
     5761 tcacggtggt acagaggagg aggcccgtga gggcgatgtt cctcacgatc cgggaggtct
     5821 ccaggtggag ccgggtctcc tcttcctgga ccgactggag cgctttcccg atcttcccca
     5881 tctcggtccc catgccggtg tccatgacct cggagatccc ctgcccctgg acgacgaggg
     5941 tccccgcata gagccagggc tggtcgtcgc cgcccggcct ccggtccgcg ggggtctttg
     6001 ccgcgacggc cttccggacc ggcaccgact ccccggtgag gagggactcg tccacgagga
     6061 ggttgttctc cgagaggagg agggcatcgg cggcgacccg gtccccctcg gagacgacca
     6121 tcacgtcctt ccgcaccacc tccctccccg cgatcctctg gagcgagccg ttcctgatga
     6181 cgagggcccg ggggctcgac aggttccgga gggcctcaag ggccttctcg gtcttctgtt
     6241 cctggtacgc cgtgatgccg atgatgacga ggacaaagga gagaaggatg gccccctcct
     6301 cgacatcccc gaggatgaag tagatgaggc ccgcccccac gaggaggagg aacatcggct
     6361 cccggatgac ctccccgacg atggcgagga ggttcttccg ctcggcggag gggatctcgt
     6421 tgtacccctc ctcgaggagg cgccggtggg cttcatcggc ggagaggcct gagaagtcgg
     6481 agaagttcag ggcaggcatc ctacgtccgt aacctgtatg attcgccggt atcccccaaa
     6541 ggtgttgcga tcaggcgccc ggaccggcgg gtccggtccc ggcctcctcc tcctttcggt
     6601 attcccggta cgagtacacg acgagccaca cggagatgac gaggaccggc ccgaggatga
     6661 agaagaaggc aagagcggga aaggccgccc ctgccaggca caggatccct gcgatccgga
     6721 agagtttccc gccgagagca tgggtccggt cccagaccct ttccgaggat agggtccagg
     6781 gggtgcggat cccgatgaac cagttcctcc tcgcccgtcc ggtgaggatc ccgatggtgt
     6841 agaagaggac cccgaaggca ggcgagagga cctgggagaa gttgaacctc gcacccgtgt
     6901 tccagaggat gacgaggatc tggaggtaga ggaggaagag gaggaggacg gcgatgaagt
     6961 gctcgtaatc ggacctgaac ttcctgatgt tttcacgcag cgggtcgatc cgggggatgg
     7021 cgaggagcag gatggcgagc cccgcggaga tgaggggcac gaaaaagagc ccccaggcct
     7081 tcggcatgga tccgttcggc tcgccataca gtccccagtg cgtgacgatg cgatcgggga
     7141 ggtacgggta cgcggcgacg gagagggcga aggtgaggat gaggatccct gagatgatcg
     7201 caaggttcca gcggggatgc atagagagaa tggatcaccg gcccttttca gggttcccaa
     7261 aaatccgcgg gttggagagt cccctctcgg cccccgatca ctcgggatga agggccctca
     7321 ccctcccgaa cttcgtttcg taggcagcaa gcgcccgggg ggcgccgagg agaagccgcc
     7381 gcaccgtgtt gtgcacgacc gggcttccga ccgggtcgaa ggtcccgagg agatccacaa
     7441 ggatcccggc gagagggagg tagtattcct cctccatccg gtccaggtgg gcgaggggct
     7501 ggagggcccg gaccgcggag gtctcgttca cccgcgccag cttctcgagg atcgagagga
     7561 ggagggctct tccgtctgcc atctggagag agaccttccg gttcatggca aaccggacat
     7621 agagggctcg ctggtcattg gcctgctcga tgcgaaaggc cggcgccgtc tcggcacgcc
     7681 gcacgagctc cacgagatcc ggggcattcg agccggcaag cgtagagagg aacgcctccc
     7741 aggcaaccag gttccccgac gactcggtga ggaagtcctc gaagacggga aggcggtcgg
     7801 gggaggagga cgcgaggagg agccggaagg cgttgagccg gtcgctcgcc gaatgcgatt
     7861 cgcggaactg ccggaggagc agagcatgga cctccggggc gtccagggtc gagaggatct
     7921 cgagaaggag gttcttcacc tgccgcctct tcacggcccg gatccggtcg gggacggcgg
     7981 cggggaccgg gtccccggca tactcacggt agagggcgag ggcggcatcc cggtgcctcg
     8041 ccgcgatggc cgagaggatc cgctgtctcg cctcgaagag ggcgcggtac cggtgggccg
     8101 acccctcgtc ctccaccgat tcgaggatgg tgagcccctg cccgccagcc cgttcgagcc
     8161 accctgtatc cgcgaggagg tccatgaaga gggaggtgaa cctctcggag ggggttgcgg
     8221 ccgggtcggc gaggagccgg gccatctccc ggtccgtgag gccatggaag gcgatgaacc
     8281 ggttcacggc gtccgggtcg gtccgggcct ggagatggag ggcgttttcc cccgggtcgt
     8341 acttcaccct gccatagaag gagtacccgc ggttcaggga ggcataggcg gggcggtcca
     8401 cgcccctgat cgcgatctcc tccctcgttc cggcgatcct ccgcatcccc tccgcgagaa
     8461 ccttcccgtc ggaggacaga agggcatagg tgaaggggaa ctcccaggcc ttcccctctt
     8521 tcgatggcga tacctgcaag gtgagggtga agctccgggc ctctccgtcg tatgaaccct
     8581 ccgcggtcac cacggggtag ccggtctcct tcagccaggc ccgggcaaat ccggccagat
     8641 caccgccccc cgtctcctcc atgcatttga cccagtcggc ccgggaggcg ttgccgtgcc
     8701 ggtaccgggt gtgatagagg tggaggcccc ctgcaaaggc ctccttcccg aggagggtct
     8761 ccaccatccg gacgaactcg ggggctttca cgtaggtgac ggcggtgatg aggtcgttcg
     8821 ggtcgttgaa gccgtcgggc tctatcggca tcgccatcgc cccggtgtcg agggccaggg
     8881 tccccccgtc gggggtgagg agggtgagag cggtcccgag ccggttgtag tccgggccga
     8941 agaggaagtc atggttctgg ttctcgacga agaccgtgac cgcctcgttc agccagagtt
     9001 cgaagggcga cctcccggtc acctcggagc cgttcaggtt atggtagtac tcgtggacct
     9061 tcacccggaa gaggtactcg taggccgaat cggtcatctg gggaaagggc atgatccggt
     9121 tcgtggtgat ggtcgtgttc cccacgtttt ccatgccccc gaagtcggag ttctgcatcc
     9181 cgatctcacg gtagaccgtc ccggtgtact ggtaccccgg ggtgatgcgg gacacgtagg
     9241 tcttgaggag gccccggata tccccgatcc ggcggggatc tccgccggcg gtcttcaggg
     9301 cctcccgctc ccggatgagc cggtacatct ccttccggat caccaggttg cggtactgtt
     9361 cggggccggt aaagaggtac acccagatga ccgcgtcgtg gagaatcccg agggcctccc
     9421 gggcgatgga ggggtcggag tccggcggca ccaggagctc gagcatgaag gtcttcccgt
     9481 ccgggtactc gacctgccgc tcgaaggtcg cgtaggtgcc taccccgagg aagaagaggt
     9541 acggcgccat gggggtgacc atgttcctgt accggatcct gtcccggccg ggttcgacct
     9601 ggtgcctcgc ctcggcgata tcgccgttcg agatgaggtg ggtataccgc gaatcggcga
     9661 tgatggtggt caggtacgtg cacttcgcgg tcatatcgtc gatgcagggc accaggcgct
     9721 ggaatcccca ctgctggcac tgggtgatct gtgtgggggg tgcccccggc ggggtctcgt
     9781 cgtagtagag cccctccagg atgttcctcg tgggccggca gacggtctcg gtgcggacgg
     9841 tgaactccgt tcccggggtg agacggggct ggaaggtgag ccggatcttc gactcggccc
     9901 gccggtaatc cgccgtgacc ctgcgtccct ccgaggtgat ggagacgatc tcgaggtctt
     9961 ttgcgttcag ctcgagggtc tggagggtac ggtcaagggt acgtgcggtg aggtaagaga
    10021 tgacccgggt gtgatcgtca tggacatcga aggtgaggtc catgtggagg accttcaccc
    10081 gcagctcgcc gaagtcctcg gggtaatacc ggaagatccg gttccccttc ccgccctcat
    10141 caccgtggcc ggggccaact gcatctccca taaaccaacc cgttcccttg ggttttcagc
    10201 ggggatgagt gataatcctt ccctctccat cttttgcgtc gggaccggct aatctctccg
    10261 cccgtactgg ttgatgagcc ggatcaggtt cccgagtccc tcgagctcca tctcgaggac
    10321 ctgggtcctc gtcatcccca tggaggtttc gccgtcggcg gtgagggttt ccggcccccg
    10381 caggacctcc cgttcccggc cgtccgccgt gagcacctcc cgtgcctcct catcgtggac
    10441 aaaggcccgg cccgggaaga tcaccacgtc ctcgagaccc gaaagatcga tgcgctccag
    10501 gtcgtcgatg gtgatcaggc aggcgatgtc cttctgtgcg gcgatgaccc gggagcggta
    10561 cccccggcga tccaggatct cctggacgaa gggcgccgcg acccggccgc tgatgaccgt
    10621 ggcccggccc gtcacccggg ggagccggtc gaggagcccg tcctcggaaa ggatggcgaa
    10681 gggggaaccg atttccgggt cccagagagg cgttcccgtg atcttcatcc ggaactcctt
    10741 cgcaagaccg gtgaccatgt cccggaactc ctccacggtc tgcatctcgc gccccttcag
    10801 gacgggttcg ttcccgagga tgagccccga ttcccgggtg ttggcaaacc gcatgaggat
    10861 gagccccttc gctccccgct cctcgagcca ggcgcaggtc tccccgagga cctcaccgtc
    10921 gttcaccccc gggatcacca ccgcggcccc gtacacgtcg cagcccccgc agaggtcctc
    10981 caggatcccg agggagacct cgggggtggg gtcatgcatc catttccggc gcagcttcgg
    11041 ggacgaggcg aagatggtga aggagacctc ggcgaggccg tgatcgagga ggaaccgggc
    11101 gagggagcga tcatcaaagc ccttcccgct cgtgtacccg atgtggagag gggcctccat
    11161 cccggcgatg agctccacca ggtcccggaa ccgggggtag cagcttgggt cgcccccgcc
    11221 gctgatagtg atccgggtga ggtccccctc catcatctgg aggttcgcaa ggacatcctc
    11281 ggcgatggcc gggagatccc ggaagcctgt gtaccgctct ttcaccgatc tcgagcagta
    11341 gtcgcagcct ttcctgaagg gcgggcagtg ccggcacccg aaggggggga catccttcgc
    11401 gtgtttgaag taacagtagt cgcagtagcc cctgcagtca acacccggtc tcccgccgat
    11461 gtccacggtg agctggggca tcactcctaa gtccgtgttc caaccataat gaccttgtga
    11521 tgatcccgtt gtcagaaatc agtctatcga atcgggataa taagggggat aagtccggcc
    11581 tcaccgagcc cgatcacggt ctggagaacg agcaagggaa cgatcagata gccgattacc
    11641 ctatggggga ggcggatcct ggttctcctc gcaataacca gactggggac cagctgggcg
    11701 agtgcgagga tcgcgacgac aagcccgagg agaccatgaa ctgactcaag gaccggttcc
    11761 ccgtgacccc tggtcgtcag caatcccagg acgaatgttc cgaccacgaa ggcgctgaac
    11821 catatactga gcatccggtg aaaccggaaa atatgatgga cacgaattgc ctttatacgc
    11881 cccttctgca ctgccccggt caggatcacc gctaccatga ggaccagcgc gacgatccct
    11941 gtcaccgcat gaccgatcca ccgggattct gacccgatgg taactgcgac acttgccaag
    12001 gctatgagaa atcctgaaac aaggaggata gtggggatat ccggccacgt tacatcctgg
    12061 tgggtctgct cttctggaat ggatggctcc tcccctcgaa ctccaaggga acacgtgtat
    12121 atgctgcgtc cccctatttt gcctgttcgg atccttttat tcgatggtct tccagatctc
    12181 cctgcctcaa tttccgggat gaaggggtag ctattaataa cccggcgcct atggcctcct
    12241 atcttttgga ggaaaaggaa tgaaattgaa cgtgaaggcg ttgtccatcg cttcaggcat
    12301 tgtgatcggg ttggcctctc tctttgtcgg cgaggctgca gtgttcacag ggatcgggct
    12361 tgattatgtc aatatcgccg ggccgctgca ccccggttat tcgccgaccc cgctgggggc
    12421 cctcatcatg gcgatctgga tgttcatcta tggcctcatc ggcggagccc tgatcgccgc
    12481 catctacaat cgctttgcag ggcccgacat gaagtaggca gggagaaggg tcgggagact
    12541 gtggaaaagg tgaaaggtat ggccatcgat cccgtgtgca agatggaagt ggatgagaac
    12601 cgggcaaagt acgggaccag gtacaagggg aagtcatatt acttctgtac cgaggggtgc
    12661 aagaagaagt tcgaggaaga tcccgagaag tattcccgca tggtcactga aatcggcgat
    12721 tcaggcggaa aatgccactg agaggataag gcatggacgg attctggtac ggcatgatgg
    12781 atggataccc ctatcccttc tacggaatgt ttatctggat gggtatcgtc tgggttctcc
    12841 agctcgttgt cggttatttt gtcttccggg acgccaggga acgcgggatg agccccgtcc
    12901 tctggttcat tctggtcgtt ctccccatga tcggatggct gttcctcgtg atctatgtcg
    12961 tcatccggga gaccggccgg cctgcatcgt cgggcgagaa gtcctctgcc gggggtatcc
    13021 tcgatgagag gtttgcgaag ggggagatca ccgcggagga ttaccggaag atgaaggaag
    13081 agctgaaccg gtagggatcc gatggaaatg gaaggggcgc acgggagtca caccgatgcc
    13141 cacgccggac acggcgttgg cggggaggaa catcacccgg tgaccggccc gggtgccccc
    13201 ggggggcacc ggccggggat gcatggtgct caccgtcaca tgctcgagga cttccggaga
    13261 cggttcatcg tctccatgat cctcacggtc ccgatcctca tcctgtcacg gtcattccag
    13321 tccttcttcg gcgtttcgtt ctctttcccg ggatccgacc tgattctcct catcctctcg
    13381 acggcggtgt acgcgtatgg cgggttcccg ttcctgaagg ggatcatgga ggagctccgc
    13441 accaggctcc cgggcatgat gacccttatc gcggtcgcca tcaccgtcgc gtacgggttt
    13501 tccgcggcaa ccgtgctcgg cctcgtcacg ggcgaggcct tcttctggga gctcgccacc
    13561 ctgatcgaca tcatgctcct cgggcactgg atcgagatgc gttccgtgct gggcgcctcc
    13621 cgggccctgg aggagctcgt gaggatcatg ccctcgacgg cccacgtcc
//
LOCUS       DATGDI010000204         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_429493, whole genome shotgun sequence.
ACCESSION   DATGDI010000204 DATGDI010000000
VERSION     DATGDI010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429493"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VKO45_07645"
     CDS             complement(<1..815)
                     /locus_tag="VKO45_07645"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HMA05785.1"
                     /translation="MTGRTIFISLALVCMAAAVLFAGCTTPGGNIPATPVPTTPVPAT
                     TQRPAGTGSFDQSNDGGTYPISRGAEIELRLPENPTTGFSWNLSLSTGLSLINDSYIP
                     SDTTGKLVGSGGTHVWFLSANLPGGQTVSGTYRRPWEPAGPGVTFFTLNLIVEEGSCG
                     PNVCTLPTTPSTVPPAYPVYTEADTGKAVQESRGGTFAIRLPENPSTGYSWNLSLPAG
                     ITLARDEYIPPQTAGQTVGRGGTRSFTLVADKTGTGNITTEYRRPWVPAGTVTR"
     gene            complement(928..1473)
                     /locus_tag="VKO45_07650"
     CDS             complement(928..1473)
                     /locus_tag="VKO45_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05786.1"
                     /translation="MPRDALDTLGLRRPGRAPAGILFLLVILPVLIGWLAGSPPHAVL
                     GFILSILLLQGLAPTVGMTLGLKVIPLLLILASVATGVILAIFMVCDLFSGKSERISR
                     WITGIQGTMERYRFLTTYGEFMLIPIMWVPGIGLYGTPVIAWVLWWRELRAILLMLVG
                     WVIACLTVVGVTEGILAALGL"
     gene            complement(1589..1864)
                     /locus_tag="VKO45_07655"
     CDS             complement(1589..1864)
                     /locus_tag="VKO45_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05787.1"
                     /translation="MPETFRLIDRCPGSAGIRTPTLTVKRCPRCGEEIEIFSNEVSAP
                     CGKCGLVIYNDTASCIRWCRYAEECVGPETVRKFREGGRERDGERGG"
     gene            complement(1873..2187)
                     /locus_tag="VKO45_07660"
     CDS             complement(1873..2187)
                     /locus_tag="VKO45_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMA05788.1"
                     /translation="MRLADLVAYQDGTVASRMIVFRDAGNITLFSFDKGEGLSEHTAP
                     FDAVVTILDGDCEVWIAGETHHLSEGETIIFPANKPHALSAVTRFKMMLTMIRECPTP
                     GA"
     gene            complement(2306..>2491)
                     /locus_tag="VKO45_07665"
     CDS             complement(2306..>2491)
                     /locus_tag="VKO45_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA05789.1"
                     /translation="LAGLASLLKLRRNPSCIECGKCEFACPVDEAKREDLKGECYLCG
                     RCTGVCPVEGAITYRRA"
BASE COUNT          436 a          804 c          820 g          431 t
ORIGIN      
        1 cgggtcacgg tgcccgcggg cacccagggg cgcctgtact ctgtggtgat attcccggtg
       61 ccggtcttat ccgccaccag ggtgaaggac cgggtgcccc cccgtcccac ggtctgcccc
      121 gcggtctggg gagggatgta ctcatcccgg gccagggtga taccggcggg aaggctcagg
      181 ttccaggaat aaccggtcga ggggttctcg ggaagccgga tggcgaaggt cccgccccgg
      241 gattcctgca cggccttccc ggtatcggct tccgtgtaca ccgggtacgc cggaggaacc
      301 gttgagggcg tggtgggaag ggtgcagacg ttgggtccgc agctgccttc ctcgacgatg
      361 aggttcaggg tgaagaaggt cacaccgggc ccggcaggct cccagggccg ccggtatgta
      421 cccgagacgg tctgtccgcc cggcaggttc gccgagagga accagacatg agtccctccc
      481 gacccgacga gcttccccgt ggtatcgctg gggatgtagg agtcgttgat gagggagagg
      541 ccggtactaa gggacaggtt ccaggagaac cccgtcgtcg ggttctccgg gagccggagc
      601 tcgatctccg caccccggga gatcgggtag gttccaccgt cgttcgactg atcgaaggaa
      661 ccggtacccg ccggtcgttg cgtggttgct ggtactggtg ttgtcgggac gggtgtggcc
      721 gggatattcc ccccgggcgt ggtgcatccc gcgaacagga ccgcggcggc catgcacacg
      781 agggcgaggg atatgaagat cgttcttcct gtcatggtcg atctcccata tttagcccgg
      841 attcacataa atcttccata aacaacgaaa tttttcgcag tctcccgggc ggaaccccgg
      901 aggtcccgtc ggaaggtcgg atccctctca cagccccagg gccgcgagga tcccttccgt
      961 gacgccgacc acggtcaggc atgcgatgac ccagccgacg agcatgagga ggatggcccg
     1021 gagctccctc caccagagga cccaggcgat gacgggggtc ccgtagagcc cgataccggg
     1081 gacccacatg atggggatga gcatgaactc cccgtaggtc gtcaggaacc ggtacctctc
     1141 catggtcccc tggattcccg taatccaccg ggagatccgc tccgatttcc cggagaagag
     1201 atcgcagacc atgaagatgg cgaggatgac ccccgtggca accgaggcca ggatgaggag
     1261 aagggggatg accttgaggc ccagcgtcat accgacggtc ggggcgagac cctggaggag
     1321 gaggatggaa aggatgaaac cgaggaccgc gtgaggggga gagccggcaa gccacccgat
     1381 gaggaccggg aggatcacga gcaggaatag gattcctgcc ggggccctgc cggggcgcct
     1441 gaggcccagg gtgtccaggg catcacgcgg catgcgggga gatccctcgg gaggtacggg
     1501 taaggaggat gcctggccag gatgatccga aagatcggcg gacctctcct ggtttccccg
     1561 gtcgcccacg gggtgcaggt tttgcccgtt acccgccacg ttccccgtcc cgttccctcc
     1621 cgccttcacg aaacttcctc acggtctccg gccccacgca ttcctcggca tagcggcacc
     1681 agcggataca cgaggcggtg tcgttgtaga tgacgaggcc gcacttcccg cagggggccg
     1741 agacctcgtt cgagaagatc tcgatctcct ccccgcacct cgggcatctc ttcaccgtga
     1801 gggtcggggt ccggattccc gccgatccag ggcaccgatc gatgagccgg aaggtctcgg
     1861 gcatccggcc tctcatgcac ccggcgtcgg gcactcgcgg atcatggtga gcatcatctt
     1921 gaaccgcgtg accgccgaaa gggcatgagg tttgttcgcg gggaagatga tggtctcgcc
     1981 ctccgaaagg tgatgggtct ccccggcgat ccagacctcg cagtccccgt cgaggatggt
     2041 caccaccgcg tcgaaggggg cggtgtgctc ggagagcccc tcccccttgt caaaggaaaa
     2101 gagggtgatg ttccccgcgt cccggaagac gatcatccgg ctcgcgaccg tcccgtcctg
     2161 gtacgccacg aggtccgcga gacgcatgac cttccccagg agctcctccc gggatccgtg
     2221 ctccttcttt tcatcgctca atcggatctg cctccggaat ctcttctcac tcccgggcca
     2281 tagccgtttc cggcctcccc ctctctcacg ccctccggta cgtgatggcc ccttccaccg
     2341 ggcatacccc ggtgcacctc ccgcagaggt agcactcccc cttcaggtcc tcccgcttcg
     2401 cctcgtcgac cgggcaggcg aactcgcact tcccgcactc gatgcaggag gggttccgcc
     2461 tcagcttgag gaggctcgcg agaccggcga g
//
LOCUS       DATGDI010000205         1640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_313111, whole genome shotgun sequence.
ACCESSION   DATGDI010000205 DATGDI010000000
VERSION     DATGDI010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313111"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="VKO45_07670"
     CDS             complement(<1..1054)
                     /locus_tag="VKO45_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05790.1"
                     /translation="MRLTAKERILLHLLECSQSADEAEVSVDLAQEGVARGARIELRH
                     LAQFVRPLIGEDLVRERRAHVVGIRQRRKVFSLTPSGRTMALRLREKVTTQKVRIRDG
                     GSVRDGSLQEVLQGVGSRTRLLEAIRLVEQAGVLDLETVRHPAESGLVEQTWDAPRVS
                     TFVGRRRELEEVTSEEGGPRVFVVRGIAGVGKSYFAAKACELLRERRNLFWHRIRPWE
                     TSQTVLASLGGFLEALDRPGLASILTRGEPQLAGEVLRQDLPDTHAFLVFDDAHEASA
                     EAALVFRMLADAVVSAPDVRVLLLSRRALPFYSRRDVALERRVREIELGGLDSEDAAA
                     LLGVGVTMAVPAGLGRR"
     gene            1236..>1640
                     /locus_tag="VKO45_07675"
     CDS             1236..>1640
                     /locus_tag="VKO45_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05791.1"
                     /translation="MMKKGWATAFAASMLALVLVSTPAVSAAEDEPLQGAMDAAFDLT
                     NYPACREDDSCFWVGTVSGDLVGGIEIHELWDQNYVVGKPGALLGIEHFFETFTIDLG
                     SGRWVSGVDAGVWNQETGKFRANGWVMDASAGL"
BASE COUNT          305 a          537 c          527 g          271 t
ORIGIN      
        1 gccgccggcc gaggccggcc ggaacggcca tggtcactcc cacgcccagg agggccgccg
       61 catcctcgga gtccagtcct ccgagctcga tctcgcgtac gcgtcgttcc agggcgacat
      121 ccctgcgact gtaaaaggga agggcccgcc gactgagaag gagaacgcgc acgtccggtg
      181 cagagacgac ggcgtctgcc aacatccgga acacgagagc ggcctccgcg gacgcctcgt
      241 gcgcgtcgtc gaagaccagg aaggcatgcg tgtcgggcag gtcctgacgg agcacctccc
      301 cagcgagctg gggttccccc cgcgtcagga tcgaggcgag gccgggccga tccagtgcct
      361 ccaggaagcc tccaagactg gcaaggacgg tctggctcgt ctcccaaggt cgaatccgat
      421 gccagaatag gttcctccgt tctcggagga gctcacaagc cttggccgcg aagtaagact
      481 ttccgacccc agcgattcca cgaaccacga agactcgggg accgccctcc tcgctcgtga
      541 cctcctccag ctccctccgt cttcccacga aggtgctgac gcgcggagca tcccaggtct
      601 gctcgacgag gcctgattcg gcaggatgcc gcactgtctc aagatccagc actcccgctt
      661 gctcgaccag ccgaatcgcc tcaaggagac gagtccggga gccgacgccc tggaggacct
      721 cttgaaggct cccgtcacgc actgagcccc catcacggat gcggaccttc tgggtcgtca
      781 ctttctcccg caggcgaagc gccatcgtgc gaccggaggg cgtgagcgag aacaccttgc
      841 gccgctggcg gatcccgacc acgtgggccc tccgctctcg gacgaggtcc tccccgatga
      901 gcgggcggac gaactgagca agatggcgga gctcgatcct ggccccgcgg gccacgccct
      961 cctgggccag gtcgacggag acctccgcct catccgcgga ttggctgcat tccagaagat
     1021 gcaggagaat gcgttccttg gccgtgaggc gcatccctct cagccccccc gtgctaaagg
     1081 ccaacaaggt gtcgctgtat gaaggttgtg aaccgactct agggactcca atgggattcc
     1141 acagggtccc tcatgcccgt atggagcctt ccaccgccag gatgatggag agtcgcgtcg
     1201 aaactcaagg cccgcgacgg cggggggagg tgactatgat gaagaaaggt tgggcgacag
     1261 cctttgccgc gagcatgctg gccctcgtcc tcgtatcgac ccccgccgtt tcggcggcgg
     1321 aggacgaacc cctgcaaggg gcgatggacg ccgcgttcga cttgaccaac taccccgcct
     1381 gcagggaaga tgattcctgc ttctgggtgg gaacggtcag cggtgacctc gttggaggca
     1441 tcgagattca cgaactctgg gaccagaact atgtggtcgg caaaccggga gctctcctcg
     1501 ggattgagca cttcttcgag acgttcacga tcgacctcgg ctccggaagg tgggtctcag
     1561 gggtggacgc gggcgtttgg aaccaggaaa cggggaagtt ccgggctaac ggttgggtca
     1621 tggatgcctc tgcaggtctg
//
LOCUS       DATGDI010000206        10984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_255290, whole genome shotgun sequence.
ACCESSION   DATGDI010000206 DATGDI010000000
VERSION     DATGDI010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 10984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10984
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255290"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VKO45_07680"
     CDS             complement(<1..391)
                     /locus_tag="VKO45_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA05792.1"
                     /translation="MRRSLGIILPILLLVGWEIGAVIINNPFILPPLETVIPVLLHPF
                     EDILGTGSLFYNAFVSLTRVGFGFLVAVSIAVPLGIGMGWSRTLDAFLDTTIQLLRPI
                     PPLAWGPLAIAWFAVGIYSMGFIIFLGA"
     gene            complement(397..1599)
                     /locus_tag="VKO45_07685"
     CDS             complement(397..1599)
                     /locus_tag="VKO45_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA05793.1"
                     /translation="MKGVHLLLIGCLAVLLIAAGCTTQQTTADVGILYTKGTGPMPTL
                     LATKQIDGYIAWQPFVEVAPVAHIGKVITYSGDLPPDDRWKNHPCCVLAARTDMMAAN
                     PELVNDLGAATILATRYVNDHPSEGADLVADWLAGRGNFTYGNVTVSSVEVLDRAFPT
                     VKFVNEPTEQWMAGQVEFVHALRELGAVTGSLANTTDGQSKAILFDTGPYNAAMAMID
                     AKSFKVPTPAKAPVGVGYLLSDHDASLFVAVKKWQYFNDTYGIALRPRDLAATRPDTV
                     DLLIAGKPVAELKLIPADAGPQLMQLAATNAIQVAYVGNPPTIAAIDKGTPVKILMAL
                     NDEGSGVVVVTDSPATNWETFVAWAKERAAAGKPIKMAAPGKGSIQDVMLRYALEESG
                     LSVKEVQA"
     gene            1809..2345
                     /locus_tag="VKO45_07690"
     CDS             1809..2345
                     /locus_tag="VKO45_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMA05794.1"
                     /translation="MVRLDPIDRLLRASMRSDEDFVATLNEILKSDLRMTGRELSERS
                     GITASSLYKILRGERSPNLSTLRAIVGALRHVSHPAGEEEFIGVIAARQTLDGIEDRV
                     AEVDGRRVRVREYAVHTIEDAIVAAVRAEREGALGIVCAPIVSSTIEELVSIPVATIV
                     PRESVQQAIELAARKAWH"
     gene            2378..3856
                     /gene="argH"
                     /locus_tag="VKO45_07695"
     CDS             2378..3856
                     /gene="argH"
                     /locus_tag="VKO45_07695"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HMA05795.1"
                     /translation="MKEDRIRRGRLAGERGCGVSRFLSSMDADRWIAEADVLVDMAHL
                     LMLRRQNLIGGEHARAIMDVLLGFAEKGLPTDVFDDRYEDIHAGIEAYLIDHLGEETG
                     GRIHAGRSRNDEVAACIRIRAREEVLRLMTDLNRLRGILLALGGEHTGTVMPGFTHLQ
                     HAQPTTLAHHLLAYEEAFSRDLARLRSAIGRVNRSPLGAAAFASTGFPIDREYTRELL
                     GFEGLVENSMDAVSSRDFLLEVLSASAILSTTVSRLSADLILWASPLAGFVTLSDEFC
                     STSSIMPQKKNPDTLEILRAKSGTAAGALAAALSIVKGLPMSYNRDLQEATPHLWQGI
                     SAAGDGIPILTEALATAAFHPGRMEEEAGKGFSTATGLADLFVQDLGLPFRTAHTIVG
                     RAVKAGSLDLKILEREGKKAFGISLTDLGLTREKVKKALDVRSMVDARSVTGGPAAAT
                     VKKAIRARKELLRKDEADTESLEAGLAEARGYLLAEARRLRG"
     gene            3853..5088
                     /gene="gatD"
                     /locus_tag="VKO45_07700"
     CDS             3853..5088
                     /gene="gatD"
                     /locus_tag="VKO45_07700"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HMA05796.1"
                     /translation="MTEFQTGDRIRYKGDDREYAAIYITGRDGKAVVKLDSGYNIGVP
                     FGRITWQERPKPETLALSRPVQRDDLPELAIISTGGTIASRVDYRTGAVTSQFSAEDI
                     LRAIPELASVGRFRTEQISSILSENMTMGIWQDLARAVFREVKSGVTGVIVTHGTDTM
                     AYTAAALSFMVETPVPVVLVGSQRSADRPSSDNAMNALCAASAAVSPLGEVAVVMHAT
                     TSDDRCAIHRATRVRKMHSSRRDAFQSLGMEPLGHVGYPDLTVSLSPEAAARGGREPA
                     LKEKMEARCGLISFYPGTAPSILEAYRGCRGLIVAGTGLGHVSTSWVKGLAALVQDGT
                     SVVMTSQCLHGRVCDRVYDTGRDLLAAGVMEGEDMLPEAALVKLMWVLGQTEDDREIR
                     ALMAENLRGEIRRRSLHGL"
     gene            5078..6976
                     /gene="gatE"
                     /locus_tag="VKO45_07705"
     CDS             5078..6976
                     /gene="gatE"
                     /locus_tag="VKO45_07705"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HMA05797.1"
                     /translation="MASDVPRLRAGLSGRPPAALPRPPLRAGLEIHQQLDTAEKLFCH
                     CPTCLRDTKERNGEFTRFLRATESEMGEIDRAALEEMRTVRRFTYYTYDTTCLVEDDE
                     EPPAPLNPEAVATCLTIARCFGMHPVEQVHTMRKIVIDGSNTCGFQRTALLALGGSLP
                     GGARIETICLEEDAAQRVTDQVFSLDRLGIPLVEITTGPDLRTPEEVQEVAEYIGMVL
                     RSTGKVKRGKGTIRQDINISIPGGARVEIKGVQELGLIAEVVRREMQRQENLIAIRDE
                     LKGRSASPDEDPVEVTALFGGTKSSILKKARVILAVRLRGFAGLVGKEIQPGRRLGSE
                     LSDYAKRCGVGGIFHTDELPAYGITAGEVRSLRKMVDAAGDDAVVLVAGTAEEAGCAA
                     RQVVRRAAMALSGVPEETRKMLEEGSTAYMRPLPGAARMYPETDIFPVTVEKEFWEGI
                     AVPELLEAKAARFARELGLEPALARQAAFSENLPLFEEAIRRGIKATLAFRVIITTPV
                     ELARKGVEVWRVTGADYLALLAAIESGRAAKEAAPDLLAAVAGGMTPDRAIASLVTSV
                     TPGDLTRLIRGVIDGRKDFVVEKGMAALGPLMGLVMQEVRGKVDGKLVSEALRGELER
                     FLREKSRE"
     gene            7018..7311
                     /locus_tag="VKO45_07710"
     CDS             7018..7311
                     /locus_tag="VKO45_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5350 domain-containing protein"
                     /protein_id="HMA05798.1"
                     /translation="MGKTGTVNWMQVKGVKGQVRLVPQKDAEVKKPGPNQRFKPNAGT
                     RFKKAEEEMARQGGQRGGRGGRGRGGREKDPRLRRRIRRSKSAIMGAKSKAKK"
     gene            7368..7724
                     /locus_tag="VKO45_07715"
     CDS             7368..7724
                     /locus_tag="VKO45_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05799.1"
                     /translation="MDDKTAVKEYQFGERAKSELILVSQLLLVLGEMKDREFSGGKRI
                     LLALMEGIRSELEFAHRGTGAMEFQRAVNHLNEAIGIVESGQPGPATLKIADAISDAT
                     TAAQAAWQVLSAHGLL"
     gene            7711..8238
                     /locus_tag="VKO45_07720"
     CDS             7711..8238
                     /locus_tag="VKO45_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMA05800.1"
                     /translation="MDSSSHLAFLSSRASVRDFTPAPLSEPEIEAILRSARTAPSAGN
                     REAWDVVVVTDGEVREALAEAALSQPHVREAPCVLAVCANYIRSMSRYGERGILYAIQ
                     DATIACTYMMLAAHAAGLKSCWTGAFDESEVRDALGLPEHIRPVALLAVGRGRDPAER
                     TGRMDLSEHVHRDRW"
     gene            8251..8589
                     /locus_tag="VKO45_07725"
     CDS             8251..8589
                     /locus_tag="VKO45_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF555 domain-containing protein"
                     /protein_id="HMA05801.1"
                     /translation="MPDYMVTLESAWIVKDAGSVDDALGIAIAEAGKRLNPTAKFVEI
                     ETGLLPCPFCRKDLSSALVVAGTGLVGLLLQMKVFRAETAEHAARIARSVIGKALRDI
                     PLSVLEVRQL"
     gene            8622..9485
                     /locus_tag="VKO45_07730"
     CDS             8622..9485
                     /locus_tag="VKO45_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HMA05802.1"
                     /translation="MLRVPRFPDRNGSTFVSGHQVFFGGGAANTAAGIARLGAPVTLV
                     SAVGEEFAGSNYERWLSGLGVALDLVVAPGKRTPRAFVFTDDGGDQVTYFEWGASETF
                     AAREAPSLDQVHLATADPGFNVRVAERGRLVSFDPGQDIIWYTADQIRRILARVRILF
                     ANRHEMIHLSRILGVPREDILGQVPVVVVTLDAEGSLLYTGGREHRIPVVPADVVDPT
                     GAGDAYRAGFLAAYHRGYSPLTSCRVGATTASFAIEKAGCQTNLPDWNRMTARYWTHF
                     GDLPSAGERGT"
     gene            9543..>10984
                     /locus_tag="VKO45_07735"
     CDS             9543..>10984
                     /locus_tag="VKO45_07735"
                     /inference="COORDINATES: protein motif:HMM:NF021370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HMA05803.1"
                     /translation="MASSPDARMEHLTRFLFTAPPWYVAGGLAVLAGLALDIARGRMP
                     GPFSLGILAFTAPALLALVLTKPLVTLAGSFTTWNRSASLALASLVFSACTVIPGLIL
                     PVPNLLAFLYAFSLGLVLGVRLLVLVATADYRPARMLPAALSQSLPGMAAGWLLFPGP
                     FTAASVVALVTVGGAALLLIALIDRPLHRIFHIHIFQFLTAFLAHLTDGSRGMEEFFS
                     ALGEEITLPQVSFLFRRAGKKDILLTVPNVHPGPLGDVGGGRLPHILHASLDGEVLSA
                     HGCATHDYNLTREEETGKIVTAVRDSLAGLQVGPAAGRSFRVREGSVDLLCQRFADSL
                     LLVGTRAPERTEDLDPAIGWILMAEGHRLFGHVALVDAHNSMTEVSGGTFLGSPVAGE
                     YITGGLRAMETAGTMERSGFRAGVAHLTLPFTREQGLGDLGVQALVIEAGGQKTAYVL
                     LDGNNIVKGGREVLLGGIRDLVDDCEVMTS"
BASE COUNT         1846 a         3709 c         3672 g         1757 t
ORIGIN      
        1 aggccccgag gaagatgatg aaacccatgg aatagatccc gacggcaaac caggcgatgg
       61 ccaggggtcc ccaggcgagg gggggtatgg gcctgaggag ctggatggtg gtgtcgagaa
      121 aggcatcgag ggtccgggac cagcccatcc cgatgcccag ggggactgcg atggataccg
      181 cgacgaggaa cccgaagccc acccgggtga gggagacgaa ggcattgtaa aagaggctcc
      241 cggtcccgag gatgtcctcg aaggggtgaa ggaggaccgg gatcaccgtc tcaagcgggg
      301 ggagaataaa gggattattg atgatgacgg ccccaatctc ccatcccacg agaagaagga
      361 tggggaggat aatgccgagg gatcttctca tgccctctaa gcctgtacct ccttcacgga
      421 taaaccgctc tcctcaaggg cgtaccggag catgacgtcc tggatggacc ccttccccgg
      481 ggcggccatc ttgatgggct tccccgccgc cgcccgctcc tttgcccatg cgacaaaggt
      541 ttcccagttg gtggccgggg aatcggtgac gacgacgacc cccgaaccct cgtcgttcag
      601 ggccatgagg atcttcaccg gcgtcccttt gtcaatggcc gcgatagttg gggggttacc
      661 cacgtaggcg acctggatgg cattcgtcgc ggcaagctgc atgagctggg ggccggcatc
      721 cgccgggatg agcttcagct cagccaccgg tttccccgcg atgagcaggt ccacggtatc
      781 cggcctggtg gcggccaggt cccggggcct gagggcgatc ccgtacgtgt cgttgaagta
      841 ctgccacttc ttcaccgcga cgaagaggga ggcgtcatga tcggaaagga ggtaccccac
      901 cccgacgggg gctttcgcgg gagtcgggac cttgaagctc ttcgcatcga tcatggccat
      961 ggccgcgttg taggggccgg tgtcgaagag gatggccttg gactgtccat cggtggtgtt
     1021 cgcgagggac ccggtgacgg ccccgagctc ccggagcgcg tggacgaact ccacctggcc
     1081 tgccatccac tgctccgtgg gttcgttcac gaatttcacc gtggggaagg cccggtccag
     1141 gacctcaacc gaggagacgg tgacattccc gtaggtgaag ttccccctgc cggcgagcca
     1201 gtcggcaacc aggtcagcac cctcggaggg gtgatcgttg acgtaccggg tcgcgaggat
     1261 ggtggccgcc cccaggtcgt tgacgagctc ggggtttgcg gccatcatgt cggtgcgggc
     1321 ggcgaggacg cagcacgggt ggttcttcca ccggtcatca ggagggaggt cccccgagta
     1381 ggtgataacc ttcccgatgt gggcgacggg ggcaacctcg acgaagggct gccaggcgat
     1441 gtagccgtcg atctgcttcg tggcgaggag cgtgggcatg gggcctgtcc ccttcgtgta
     1501 gaggatcccc acgtccgcgg tcgtctgctg ggtggtgcac ccggcggcaa tgaggagaac
     1561 cgcgaggcac ccgatgagga ggagatgcac acctttcatg gaggaacatg gacccggtca
     1621 gctcataaaa tattctattt cacgtgattg atatccacat aaaggacatt attaaagtaa
     1681 aaatgacaca atagatattc ttattcttcc tttatgtcca gtttccccgg gaaaaaccaa
     1741 aaagcagccc acctggattg gacaataagg tttttattcc tcaaaaaata tcctttctgt
     1801 gttcatacat ggtccgcctt gatcccatcg accgcctcct ccgggcctcg atgcgctcgg
     1861 acgaggactt cgtggcgacc ctgaacgaga tcctgaagtc cgacctgcgg atgaccggcc
     1921 gtgaactctc cgagaggagc ggcatcaccg cctcctccct ctacaagatc ctccgggggg
     1981 aacggtcccc caacctttcc accctccggg ccattgtcgg tgcgctccgg cacgtctccc
     2041 atccggccgg cgaggaggag ttcatcgggg tcatcgcggc gcgccagacc ctggacggta
     2101 tcgaggaccg ggtggccgaa gtggacggga ggagggtcag ggtgagggag tatgccgtgc
     2161 acaccatcga ggatgccatc gtcgccgcag tccgggccga gcgggagggg gctctcggta
     2221 tcgtctgcgc ccccatcgtg tcctccacca tcgaggagct cgtctccatc ccggtggcga
     2281 ccatcgtgcc acgggagagc gtccagcagg ccatcgagct cgctgcccgg aaggcatggc
     2341 attaaggggc ggttttccaa aacatgtagg tgagaggatg aaagaggacc ggataaggag
     2401 ggggcggctg gcgggggaga gggggtgcgg ggtctcccgc ttcctctcct ccatggacgc
     2461 agaccggtgg atcgccgagg cagacgtgct cgtggacatg gcccacctgc tcatgctccg
     2521 gcgccagaat ctcatcggcg gcgagcatgc ccgggccatc atggatgtgc tcctcggctt
     2581 cgcggagaag ggtctcccga cggacgtctt cgacgatcgg tatgaggaca tccacgcagg
     2641 gatcgaggcg tacctcatcg atcacctcgg cgaggaaacg gggggccgga tccacgcggg
     2701 ccggtcccgg aacgacgagg tcgctgcctg tatccggatc cgcgcccgcg aggaggtcct
     2761 ccggctcatg accgacctga accggctccg cgggatcctc ctcgccctcg gcggagagca
     2821 cacagggacc gtgatgcccg gcttcaccca tctccagcac gcccagccca ccaccctcgc
     2881 ccaccacctc ctcgcctacg aggaggcctt ctcgcgggac ctcgcccgcc tccgctcggc
     2941 catcgggagg gtgaaccggt cccccctggg ggccgccgcc tttgcctcga caggattccc
     3001 catcgaccgg gaatacacga gggagcttct cgggttcgag ggcctcgtgg agaactccat
     3061 ggacgcggtc tcctcccggg acttcctcct cgaggtcctc tcggcatccg cgatcctctc
     3121 caccacggtg agccggctct ccgccgacct catcctctgg gcgagccccc tcgccggatt
     3181 cgtcaccctc tccgacgagt tctgctccac ctcctccatc atgccccaga agaagaaccc
     3241 ggacaccctc gagatcctcc gggcgaagtc ggggacggcc gcgggagccc tcgccgccgc
     3301 cctctccatc gtgaagggtc tccccatgag ctacaaccgg gacctccagg aggcgacccc
     3361 ccacctctgg caggggatct ccgccgcggg ggacgggatt cccatcctca ccgaggccct
     3421 cgcgacggcg gccttccacc ccggccggat ggaggaggag gccgggaagg gtttctccac
     3481 cgcgaccggc ctcgctgacc tcttcgtgca ggacctgggg ctccccttcc gcaccgccca
     3541 caccatcgtc gggagggccg tgaaggcggg gagccttgac ctgaagattc tcgagaggga
     3601 gggaaagaag gccttcggga tttccctcac cgacctcggg ctcacccggg aaaaggtgaa
     3661 gaaggccctc gatgtccgtt ccatggtgga tgcccggagc gtgacgggtg gccccgctgc
     3721 cgccacggta aaaaaggcca tccgggcgcg aaaagagctc ctcaggaagg atgaggccga
     3781 tacggagtcc ctcgaggcag ggctcgcgga ggcacgcggg tatctcctcg ccgaggcacg
     3841 gaggcttcga gggtgaccga gttccagacg ggtgaccgga tccggtacaa aggggacgac
     3901 agggaatacg cggccatcta catcaccggg cgggacggaa aggcggtggt gaagctcgac
     3961 agcgggtata acatcggcgt ccccttcggg aggatcacct ggcaggagag accgaagccc
     4021 gaaaccctgg cgctgagccg gcctgtccag cgggatgacc ttccggagct cgccatcatc
     4081 tccacgggcg gcaccatcgc gagccgggtg gattaccgga ccggcgccgt gacctcgcag
     4141 ttctccgccg aggacatcct ccgcgccata ccggagcttg cctccgtcgg acggttccgc
     4201 accgagcaga tctcctccat cctctcggag aacatgacca tgggcatctg gcaggacctg
     4261 gcccgggccg tcttccggga ggtgaaatcc ggcgtgacgg gggtgatcgt cacccacggg
     4321 accgatacga tggcctatac cgcggccgcc ctttccttca tggtggagac cccggtgccg
     4381 gtcgtccttg tcggctccca gagatccgcc gaccggccga gcagcgacaa cgcgatgaac
     4441 gccctctgtg ccgcctccgc ggcggtctcc cccctcgggg aggttgcggt ggtgatgcac
     4501 gccaccacga gcgatgaccg ctgcgccatc caccgggcga cccgggtcag gaagatgcac
     4561 agctcccggc gggacgcctt ccagtccctc ggcatggaac ccctcggcca cgtggggtac
     4621 ccggacctca cggtcagcct ctctcccgag gcggcggcga ggggcgggag ggagcctgcc
     4681 ctgaaggaaa agatggaggc gcgatgcggg ctcatctcct tctatccggg gacggccccc
     4741 tcgatcctgg aggcgtaccg gggctgccgc ggcctgatcg ttgcagggac cgggctcggc
     4801 catgtctcga cctcctgggt aaagggtctc gcggccctcg tccaggacgg gacgtccgtc
     4861 gtaatgacct cccagtgcct ccacggccgg gtgtgcgacc gggtgtatga cacgggacgg
     4921 gacctcctcg cggcgggcgt gatggagggg gaggacatgc tccccgaggc cgccctcgtg
     4981 aaactgatgt gggtcctcgg tcagacggag gacgaccgcg agatccgggc cctcatggcc
     5041 gaaaacctgc ggggcgagat ccggaggagg tctctccatg gcctctgacg tgccccggct
     5101 ccgggcaggg ctctcgggca ggcctccggc cgccctcccc aggccgcccc tacgggccgg
     5161 cctggagatc caccagcagc tcgacaccgc cgagaagctc ttctgccact gcccgacctg
     5221 cctccgggat acgaaggagc ggaacgggga gttcacccgg ttcctccggg cgaccgagag
     5281 cgagatgggg gagatcgacc gggccgccct cgaggagatg cggaccgtcc ggcggtttac
     5341 gtactacacc tatgacacta cctgcctcgt ggaggacgac gaggagcccc ccgcccccct
     5401 gaacccggag gccgtggcca cctgcctcac catcgccagg tgcttcggga tgcaccctgt
     5461 cgaacaggtc cacacgatgc gcaagatcgt catcgacggc tccaacacct gcggcttcca
     5521 gcggaccgcc ctcctcgccc tggggggttc cctccccggc ggggcccgga tcgagaccat
     5581 ctgcctcgag gaggatgccg cgcagcgggt cacggaccag gtcttttccc tggaccggct
     5641 cggcatcccc ctcgtggaga tcaccacggg gccggatctg cgcaccccgg aggaggtcca
     5701 ggaggtcgcc gagtacatcg ggatggtgct ccgttccacc gggaaggtga agcggggcaa
     5761 ggggaccatc cggcaggaca tcaacatctc catccccggc ggcgcccggg tggagatcaa
     5821 gggggtgcag gagctcggcc tcatcgccga ggtggtccgc cgggagatgc agcggcagga
     5881 gaacctcatc gccatccggg acgagctgaa gggcaggagc gcctcccccg atgaggatcc
     5941 ggtcgaggtc accgccctct tcgggggaac gaaatcctct atcctcaaga aggcgagggt
     6001 catcctcgcg gtccggctga ggggcttcgc cggcctcgtg ggaaaggaga tccagccggg
     6061 gaggaggctc gggagcgagc tctcggatta cgcgaagcga tgcggcgtcg gaggaatctt
     6121 ccacaccgac gagctcccgg cgtacgggat caccgccggg gaggtgcggt ccctccggaa
     6181 gatggtggac gcggccgggg acgatgccgt tgtcctcgtc gcaggcacgg ccgaggaggc
     6241 aggctgcgcc gcgaggcagg tggtccgccg ggctgcgatg gcactctcgg gtgtgcccga
     6301 ggagacccgg aaaatgctcg aggagggttc cacggcgtac atgcggcccc tcccgggggc
     6361 ggccaggatg taccccgaga ccgacatctt ccccgtgacc gtcgagaagg aattctggga
     6421 ggggattgcc gtcccggaac tcctggaggc gaaagcggcg cgctttgccc gggagctcgg
     6481 cctcgagccg gccctggccc gccaggcggc cttttccgag aacctccccc tcttcgagga
     6541 ggcgatccgg aggggaatca aggcgaccct cgccttccgg gtgatcatca ccaccccggt
     6601 ggaactcgcc cgtaaggggg tcgaggtctg gagggtcacc ggggccgact acctggccct
     6661 cctcgcggcc atcgagagcg gcagggcggc gaaggaggcg gccccggacc tcctcgccgc
     6721 ggtggccggc gggatgaccc cggaccgggc catcgcctcc ctcgtcacct ccgtcactcc
     6781 cggggatctc acccgcctca tcaggggggt tatcgacgga aggaaggatt tcgtggtcga
     6841 gaaggggatg gcggccctcg ggcccctcat gggcctcgtg atgcaggagg tacgggggaa
     6901 ggtggacggg aagctcgtga gcgaggccct ccgcggggaa ctggaacgat tcctccggga
     6961 aaaatcccgg gaataatttt atcagcctct tcgctcacat taacagggag tttatccatg
     7021 ggaaagacag gcaccgtcaa ctggatgcag gtaaaggggg tgaaggggca ggtccggctc
     7081 gttccccaga aggatgccga ggtgaagaag ccgggcccga accagaggtt caagcccaac
     7141 gccgggaccc ggttcaagaa ggccgaggag gagatggcca ggcagggtgg ccagcggggc
     7201 gggcgcgggg gccggggaag gggcggacgc gaaaaggacc cgaggctccg caggcgcatc
     7261 cggcgctcca agtcggctat catgggcgcc aagtccaagg ccaagaaata accagagaga
     7321 ataagaccaa acccttttta catctccgtt cccccatagt gttacgtatg gacgacaaga
     7381 cggcggtgaa ggagtaccag ttcggcgagc gggccaagtc ggagctgatc ctcgtttccc
     7441 agctcctcct ggtcctcggt gagatgaagg acagggagtt ctccgggggg aagcggatcc
     7501 tcctcgccct catggagggg atccggagcg agctcgagtt cgcccaccgg gggacgggtg
     7561 ccatggagtt ccagagggcc gtcaaccacc tgaacgaggc catcggcatc gtagaaagcg
     7621 gccagcccgg ccccgccacc ctgaaaatcg ccgatgccat atcggatgcc acgaccgcgg
     7681 cccaggccgc ctggcaggtc ctctcggccc atggactcct ctagccacct cgcctttctc
     7741 tcgtcgaggg catcggtgcg ggacttcacc cccgcccccc tttcggagcc cgagatcgag
     7801 gcgatcctcc ggtccgcccg gaccgccccc tcggccggga accgcgaggc ctgggacgtg
     7861 gtcgtggtga ccgacgggga ggtccgggag gccctcgccg aggcggccct ctcccagccc
     7921 catgtccggg aggccccctg cgtcctcgca gtctgcgcga actacatccg gtccatgtcc
     7981 cgctacggcg agcggggcat cctctacgcc atccaggatg cgaccatcgc ctgcacgtac
     8041 atgatgctcg ccgcccatgc cgccggcctg aagagctgct ggaccggggc cttcgacgag
     8101 agtgaggtca gggatgccct gggattgccc gaacacatcc ggcccgtcgc cctcctcgcc
     8161 gtcgggaggg ggagggaccc cgcggaacgg accgggcgga tggacctctc cgagcatgtc
     8221 caccgggacc ggtggtgagg gaggaaaccc gtgcccgact acatggtcac ccttgaatcg
     8281 gcctggatcg tgaaggacgc ggggtcggtg gacgatgccc tcgggatcgc catcgccgag
     8341 gcggggaagc gcctgaaccc cacggcgaag ttcgtcgaga tcgagaccgg cctcctcccc
     8401 tgccccttct gccggaagga cctctcctcg gctctcgtgg tcgccgggac cgggctcgtg
     8461 ggcctcctcc tccagatgaa ggtcttccgt gccgagaccg ccgagcatgc cgcgaggatc
     8521 gcgagatcgg tcatcgggaa ggccctccgg gacatccccc tctcggtcct ggaggtgagg
     8581 cagctctgat ccacgtcgtc ggccacacgg ccatcgacca catcctccgg gtcccccggt
     8641 tcccggaccg gaacggctcc accttcgtga gcggccacca ggtcttcttc ggcggcgggg
     8701 cggcgaatac tgccgccggg atcgcccggc tcggcgcccc ggtcaccctc gtctcggccg
     8761 tgggagagga attcgccggg agcaactacg agcggtggct ctccggcctc ggcgtcgcgc
     8821 tcgacctcgt ggtggccccc gggaagagga ccccccgggc cttcgtcttc accgacgatg
     8881 ggggagacca ggtcacctac ttcgagtggg gggcctccga gaccttcgcg gcgagggagg
     8941 ccccgtccct ggaccaggtc cacctggcca ccgcggatcc cggcttcaat gtccgggtcg
     9001 cggagagggg caggcttgtg tccttcgacc cgggccagga catcatctgg tacacggcgg
     9061 accagatccg ccgcatcctc gcccgggtcc gtatcctctt tgcgaaccgg cacgagatga
     9121 tccacctctc ccggatactc ggggtgccga gggaggacat cctcggacag gtccccgtcg
     9181 tggtcgtgac cctggatgcc gaggggagcc tcctctacac cggcggaagg gagcaccgga
     9241 tcccggtcgt tcccgccgac gtcgtggacc ccacgggggc gggcgatgcg taccgggccg
     9301 ggttcctcgc ggcataccac cggggttact cgcccctgac ctcctgccgg gtcggggcga
     9361 ccaccgcatc cttcgccatc gagaaggccg gctgccagac gaacctcccc gactggaacc
     9421 ggatgaccgc ccggtactgg acccatttcg gggatctccc ctcggccgga gagaggggga
     9481 catgatgcca cccggcacgc ccggggctat cgccgccggt gtcgttcttc gggagggacc
     9541 tcatggcctc tagccccgat gcacggatgg aacacctcac ccggttcctc ttcacggcgc
     9601 cgccgtggta cgtcgcgggc gggctcgcgg tgcttgcggg cctcgccctg gacatcgcac
     9661 gggggaggat gcccggaccc ttttccctcg ggatcctggc ctttaccgcc cccgcgcttc
     9721 tcgccctcgt gctcacgaag cccctcgtga cgctcgccgg ctccttcacg acctggaacc
     9781 gctccgcctc cctcgccctc gcctccctcg tcttctcggc atgcaccgtc atcccgggcc
     9841 tcatcctccc cgttccgaac ctcctcgcct tcctctatgc cttctccctc gggctcgtcc
     9901 tcggggtccg gctcctcgtc ctcgtggcca ccgccgatta ccgtcccgcg aggatgctcc
     9961 ccgcggccct ctcccagagc ctcccgggca tggccgcggg atggctcctc ttccccggcc
    10021 ccttcaccgc ggcctcggtg gtcgccctcg tcaccgtcgg gggagcggcc ctcctcctca
    10081 tcgccctcat cgaccgcccg ctccaccgga tcttccacat ccacatcttc cagttcctca
    10141 ccgccttcct cgcccacctc accgacggtt cccggggcat ggaggagttc ttctcggccc
    10201 tgggagagga gatcaccctt ccccaggtgt ccttcctctt ccggcgggcg ggaaagaagg
    10261 acatcctcct caccgtcccg aatgtccacc ccggtcccct gggggacgtc gggggaggaa
    10321 ggctccccca catcctccac gcctccctcg atggggaggt cctctcggcc cacgggtgtg
    10381 cgacccatga ctacaacctc acgagggagg aggagacggg aaagatcgtg accgccgtga
    10441 gggattccct ggccggtctc caggtaggcc cggcggcggg cagatccttc cgggtccggg
    10501 aggggagcgt ggatctcctc tgccagcggt tcgccgactc cctcctcctc gtcgggaccc
    10561 gggcgccgga gcggaccgag gatctcgatc cagccatcgg gtggatcctc atggccgagg
    10621 gccaccggct cttcgggcat gtggccctcg tggacgccca taacagcatg accgaggtga
    10681 gcggcgggac cttcctcggg agcccggttg ccggcgagta tatcacggga gggctcaggg
    10741 cgatggagac cgccgggacc atggaaaggt cgggcttccg ggccggcgtt gcccacctga
    10801 ccctcccctt caccagagag caggggctcg gcgacctggg ggtgcaggcc ctcgtcatcg
    10861 aggcgggggg gcagaagacc gcgtacgtcc tcctggacgg gaacaacatc gtgaagggcg
    10921 gacgggaggt gctcctcggc gggatccggg acctcgtgga cgactgcgag gtgatgacct
    10981 ccga
//
LOCUS       DATGDI010000207         1797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_167683, whole genome shotgun sequence.
ACCESSION   DATGDI010000207 DATGDI010000000
VERSION     DATGDI010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167683"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VKO45_07740"
     CDS             <1..184
                     /locus_tag="VKO45_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HMA05804.1"
                     /translation="RDEEVAARDLLGRGVGVVVINPPETMGAAESEATILTGSGTDRI
                     KGSKEEVAAAVWKALP"
     gene            166..1017
                     /locus_tag="VKO45_07745"
     CDS             166..1017
                     /locus_tag="VKO45_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HMA05805.1"
                     /translation="MEGAAVAWSPGHISGYFRRIQGATPRDTGSRGAGIVIGEGVTAR
                     AVRSRTPVVRMVLPEMPPWGDRVPLIETAMERLGVSAEVTTESPLPAGAGFGLSAAAL
                     LSSISALSALFDLALPDREIASLAHEIEVLHRTGLGDVAACQGGGIECRKGPGIGAEI
                     ERIPAPGAVLLAVSLGPIPTPGILGDPARMEAIGRAFPGRCPRDLDDLLYLSRSFAEA
                     SGLVTPRVREILGACDRAGVPASMTMLGEGVFALGGDAPRVLAPFGRVYRLSVSPRGF
                     SAGEVVR"
     gene            1014..1745
                     /locus_tag="VKO45_07750"
     CDS             1014..1745
                     /locus_tag="VKO45_07750"
                     /EC_number="6.3.2.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HMA05806.1"
                     /translation="MIPPDHPRYRSLVARERLAACAREGVVTPEGLAAHGRGEAFDYL
                     MGERTTESALRAERIAAAMLLAARSPVISVNGNTAALAAPLIAGLQEACRAGVEVNLF
                     HRSEERVEKITRLLEAAGVRVLRGKAEPLLPLSHPRALCLREGIFSADTVLVPLEDGD
                     RCEALVGMGKRVIAIDVNPLSRTARAATLTVVDELTRAIPNITAACREIGPGEGERLM
                     RSPGNTYFLKAALDEMRGYLEHALD"
BASE COUNT          255 a          612 c          656 g          274 t
ORIGIN      
        1 ccgcgacgag gaggtggcgg cgcgggatct gctcggccgc ggggtcgggg tcgtggtgat
       61 caaccctccg gagaccatgg gagcagccga gagcgaagcg acgatcctta ccgggagcgg
      121 gacggacagg atcaaggggt caaaggagga ggtcgcggcg gcggtatgga aggcgctgcc
      181 gtagcctggt caccggggca catctccggc tacttccggc ggatccaggg cgcgaccccc
      241 cgggataccg gttcccgcgg tgcgggaatc gtcatcgggg aaggggtcac cgcgagggcc
      301 gtccggtccc ggactcccgt cgtcaggatg gttcttccgg agatgcctcc ttggggggac
      361 cgggtccccc tcatcgaaac cgcgatggag cggctcgggg tatccgcaga ggtgacgaca
      421 gagagccccc tccccgccgg ggcagggttc ggcctctccg ccgcggccct cctctcctcc
      481 atctcggcgc tctcggccct cttcgatctc gcgctcccgg accgggagat cgcatctctc
      541 gcccacgaga tcgaggtgct ccaccggaca ggcctcgggg acgtggccgc ctgccaggga
      601 ggagggatcg agtgtcggaa gggacccggg atcggcgcgg agatcgagcg gataccggcc
      661 ccgggcgcgg tcctccttgc cgtgagcctt ggaccgatcc cgactcccgg catcctcggg
      721 gaccccgccc ggatggaggc catcggccgt gctttcccgg ggagatgccc cagggatctc
      781 gacgacctcc tctatctctc ccgttccttt gccgaggcga gcgggctcgt caccccccgg
      841 gtcagggaga tcctcggggc ctgcgaccgg gccggggtcc cggcgagcat gaccatgctc
      901 ggcgaggggg tcttcgccct gggaggggat gccccccggg ttcttgcccc cttcgggagg
      961 gtgtaccggc tcagcgtttc cccccggggc ttctctgccg gtgaggtggt ccggtgatac
     1021 cgcccgatca cccccggtac cggtctctcg tcgcccgtga acggctcgcg gcctgcgccc
     1081 gggagggggt ggtgaccccc gagggcctcg ccgcccacgg gcggggagag gccttcgatt
     1141 acctgatggg ggagaggacc accgaaagtg ccctccgggc tgagaggatc gctgcggcca
     1201 tgctcctggc cgcccgttcg ccggtcatct cggtgaacgg gaacaccgcg gccctcgcgg
     1261 cacccctcat cgccgggctc caggaggcat gccgggcagg ggtcgaggtg aacctctttc
     1321 accggtccga ggagcgggtg gaaaagatca cgaggctcct cgaggcggcc ggggtcaggg
     1381 tgctccgggg gaaggccgag cccctcctcc ccctctccca cccccgggcc ctgtgcctca
     1441 gggaggggat cttctcggcg gacaccgtcc tcgtccccct ggaggacggc gaccggtgcg
     1501 aggccctcgt cggtatgggc aagcgggtca tcgccatcga cgtaaacccc ctctcccgga
     1561 ccgcgagggc cgcgaccctc acggtggtgg acgagctcac ccgcgccatc ccgaacatca
     1621 cggcggcctg ccgggagatc ggtccggggg agggtgaaag gctcatgaga tccccgggca
     1681 atacctactt cctgaaggca gccctggacg agatgagggg gtatctcgag catgctctgg
     1741 attgagaagt accgtccgca gggccttcgc gaggtcgtgg ggcaggaacg ggtggtg
//
LOCUS       DATGDI010000208         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_80596, whole genome shotgun sequence.
ACCESSION   DATGDI010000208 DATGDI010000000
VERSION     DATGDI010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80596"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..918)
                     /locus_tag="VKO45_07755"
     CDS             complement(40..918)
                     /locus_tag="VKO45_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMA05807.1"
                     /translation="MDLRELARSLGIDYRGFFEEVQRRNAERILQKKKAVFPDPELLA
                     GYQFERHFAHERHSSAADQAIIESSILSRIVFLESCFSREELRDDRFIDLGDSSGIFL
                     HALRQPGTSLNISLEAATQCREKGLEAVRADISSLPFRPGAFDHVIMFQTLEHMPDPI
                     RTLRNLSELSAKSLTLSVPTVSRTTVHRRGYHPGWRDYEHHIFEFSDGDLEKVITHTG
                     LRLDRLDHAVLLDGHEGGPVDRLAMWSLGRMMASPSRNPEYHEVAQDLFWGTFKRFTL
                     LHLRKEPGSGTGSPGG"
     gene            complement(1000..2076)
                     /locus_tag="VKO45_07760"
     CDS             complement(1000..2076)
                     /locus_tag="VKO45_07760"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HMA05808.1"
                     /translation="MGAKKHLAGIERTPADRTDRIRWIRLDKNECTLELPPEVLDEIR
                     SRIDPEFLACYPEVYQLYDALSAHLRLPSEELLVTAGSDGAIRAAYDSFVEPGGEVVN
                     LQPNFAMYDVYTRLYRGNPVDVYYHPETLALDLPAVLAAIGPRTSLVVLSNPNSPTGT
                     EIPQEAVAAIAREAARHGATVLVDEAYHPFSPATSLPLLSRFENLLVVRSLSKAFGLA
                     SARVGFAAGNPALIRAMGAFRPIYEVTSYGVLCATTILGRYDLVEQAVARILGAKEWF
                     AGRCRDTGIGVLPGAANFVNVMVGPGEPSRYVRLCRDAGILIREGYPWGILADCIRVS
                     VGREEHMESLHEVIRRGRDQGAMR"
     gene            complement(2083..2820)
                     /locus_tag="VKO45_07765"
     CDS             complement(2083..2820)
                     /locus_tag="VKO45_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05809.1"
                     /translation="MIAALMIGREGSTGFPGKNTYPVLGKPLMAYPLLAALDSPSVDR
                     VYISTDSPQIASIGKQYGAEIIHRPPELCTSTALGEDAFVHGYRHIRSANPEEMELMV
                     LLFCNAPTVNSGQIEEGISVLRKNPSYDSAVTVSRYNMWSPLRARKIGDDGLLHPFIP
                     FEVFGDSKTLSCDRDSQGDVWFADMGASIVRPRCLDHLETGLLPQKWMGRCIYPLKQW
                     GGCDVDYRWQIPQVEYWLREYSPGKRE"
BASE COUNT          561 a          957 c          955 g          558 t
ORIGIN      
        1 ctccttcggg aatattggtg cgtcccgggc ccaaacggtt catcccccgg gggaacctgt
       61 tccggatcct ggctccttgc ggagatggag gagggtgaac cgcttgaaag tcccccagaa
      121 gaggtcctgc gccacctcgt gatactccgg attccttgat gggcttgcca tcatccggcc
      181 gagagaccac atggccaggc gatccacggg tcctccctcg tgcccgtcca ggagcaccgc
      241 gtggtccagg cggtccagac gaaggccggt atgagtgatc accttctcca ggtccccgtc
      301 agagaactca aagatgtgat gctcataatc ccgccagccg ggatgatatc cccggcgatg
      361 caccgtggtc cgggaaaccg ttggtaccga cagggtgagg gactttgcac tcagctcgga
      421 gaggttccgg agtgtccgga tgggatcagg catgtgttcc agtgtctgga acatgatgac
      481 atgatcaaag gccccgggcc tgaagggcag ggagctgatg tcggcccgca ccgcctccag
      541 gcccttctcc cggcactggg tcgccgcctc gagggagatg ttcagggagg tcccgggctg
      601 cctcagggca tggaggaaga taccgctgga atccccgagg tcgatgaacc ggtcgtcccg
      661 aagctcctcc cgggagaaac aggactccag gaagacaatg cgggagagaa tcgaagactc
      721 gataatggcc tggtccgcgg ccgaagaatg acgctcgtga gcaaagtgtc gctcgaactg
      781 gtagccggcc aggagctccg gatcgggaaa aactgccttt ttcttctgaa ggatcctctc
      841 cgcattcctc cgctgcacct cttcaaaaaa tccgcgataa tcaatgccca ggctgcgggc
      901 gagctccctg aggtccattt caggttcccg ggtcatccat aggctcacat acctgctcag
      961 ggaaaccctc caaccagggt ccccgggagg cgattcccct caccgcatgg ccccctgatc
     1021 acgccctcgt ctgatgacct catgtaaact ctccatatgt tcttcccgcc ctacggagac
     1081 ccggatgcag tcggcgagaa taccccaggg gtatccctcg cggatgagga tccccgcgtc
     1141 acggcagagc ctcacatagc gcgatggctc gccgggaccc accatcacgt tcacaaaatt
     1201 ggcggcgccc gggagtaccc cgatgccggt gtccctgcac ctcccggcaa accattcctt
     1261 tgcgccgagg atccgggcca ccgcctgttc cacgaggtcg tacctgccga ggatcgtggt
     1321 ggcacagagc acgccgtacg aggtcacctc gtagatgggc ctgaaggcgc ccatggcccg
     1381 gatgagggcc gggttcccgg cggcaaaacc cacccgggcc gaggccagcc cgaaggcctt
     1441 ggacagggaa cggaccacga ggaggttctc gaagcggctg aggagcggca ggctggtcgc
     1501 gggagagaag ggatggtagg cctcgtccac gaggaccgtt gcaccatgcc tcgcggcctc
     1561 ccgggcgatg gctgccacgg cctcctgggg gatctccgtc cccgtggggc tgttgggatt
     1621 ggaaagcacc acgaggctgg tccggggccc gatggccgcg agcacggcgg ggaggtccag
     1681 ggccagggtc tccgggtgat agtacacgtc caccgggttc ccccggtaca ggcgggtgta
     1741 gacatcgtac atcgcaaagt tgggctggag gttcacaacc tcaccgccgg gttctacaaa
     1801 ggagtcgtag gccgccctga ttgccccgtc agaccccgcc gtcaccagca gctcctctga
     1861 cgggagccgg aggtgggcag agagggcatc gtacagctgg tagacctcgg ggtagcaggc
     1921 gaggaactcg gggtcgatcc tggagcggat ctcgtcgaga acctcggggg ggagctccag
     1981 ggtgcactcg tttttgtcga ggcgaatcca gcggatcctg tccgtcctgt cggccggggt
     2041 ccgttcgatc cctgcgaggt gcttctttgc tcccacccgg tctcactccc ttttaccagg
     2101 ggaatattcc cggagccagt actctacctg aggtatctgc caccggtagt ccacgtcaca
     2161 gccaccccac tgcttcaggg ggtagatgca cctccccatc cacttctggg gaaggaggcc
     2221 ggtctcgaga tggtccaggc agcggggccg gacgatggag gcccccatgt ccgcgaacca
     2281 gacatccccc tgggaatccc tgtcgcagct cagggtcttc gaatccccga agacctcgaa
     2341 aggtatgaag gggtggagaa ggccgtcatc cccgattttt cgggcccgga gcggggacca
     2401 catgttgtac cgggacaccg tgaccgccga gtcataggac gggttcttcc tgagtaccga
     2461 gatcccctcc tcgatctgcc ccgagttcac cgtcggggca ttgcagaaga ggagcaccat
     2521 gagttccatc tcctccgggt tcgccgaccg gatgtgccgg tacccgtgga cgaaggcgtc
     2581 ctcgccgagg gcggtggagg tgcagagttc gggagggcga tggatgatct cggccccgta
     2641 ctgcttcccg atggatgcta tttgcggcga atcggtggag atatataccc tgtctactga
     2701 cggcgaatcc agggctgcga ggagcgggta tgccatcagc ggtttgccga ggacggggta
     2761 cgtgtttttc cccggaaaac cggtgcttcc ctcccgcccg atcatgagcg cggcaatcat
     2821 gtccccatcc tcctttcagg tctccagacc ctcttatgtc ccccgggtac cttcttcccg
     2881 tgcccaggag accatgcggc tgatcccgag ggagaggggg gtaaccggtc tccagcccag
     2941 atccttttca agcttggaga tgtctgcata taacccgaac tgatccgccg gtgtggatcc
     3001 ctcgtacctc accgggtacc cttccgggtc c
//
LOCUS       DATGDI010000209         5243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_342069, whole genome shotgun sequence.
ACCESSION   DATGDI010000209 DATGDI010000000
VERSION     DATGDI010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342069"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VKO45_07770"
     CDS             complement(<1..1029)
                     /locus_tag="VKO45_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HMA05810.1"
                     /translation="MSSIGALPPRYRITVDRDQCMLCERCVESCPYGVFRSQDGRLLI
                     DSRRCTACHRCIAMCPRDAIDLRERPVDYRTHPIWTREVREDIYNQAKTGKIILAGMG
                     NAADFPIIFDRMVLDACQVTNPSIDPLREPMELRTYIGKKPSRLELRRNGQGEVELLT
                     ELSPNLQLETPIMIAHMSYGAISRNAQLSLARAAKEAGTYMGTGEGGLHQALYPYQDR
                     MIVQVASGRFGVDINYLERGAAIEIKIGQGAKPGLGGHLPGEKVDADVSSTRMIPVGS
                     DAISPSPHHDIYSIEDLKQLVRALKEATEWKKPVFVKIAAVHNSAAIAAGIARSSADA
                     VVVDGFRGG"
     gene            complement(1026..2078)
                     /locus_tag="VKO45_07775"
     CDS             complement(1026..2078)
                     /locus_tag="VKO45_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HMA05811.1"
                     /translation="MCGIIGVIDRKKNPMDGSGIREALSQMQERGSGEGAGYAVYGCY
                     PEYRDCYALHVFFDNIHETKKPVDDLLEQWGRIVHEGQIPTYEQPNLRRIHAPWRYFF
                     KPDASLMSWSPSPEEDIITHLVMRINTDRNGALVISSGKNMGVFKASGWPEDVANFYR
                     IEEYQGYLWLGHNRYPTNTPGWWGGAHPFNLLGWSVVHNGEITSYGTNQRYIESYGYR
                     CTMYTDTEVVAYLFDLLVRKHGLSEELAVRALAPPFWDEIDRMDRRERELDTAIRLTY
                     GPALMNGPFAIVVARPDMMLGFTDRIKLRPLVAGENGDRLYISSEEAAIRRMDPEVGE
                     VSMPRAGSPVIGRVSP"
     gene            2293..3765
                     /gene="glnA"
                     /locus_tag="VKO45_07780"
     CDS             2293..3765
                     /gene="glnA"
                     /locus_tag="VKO45_07780"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HMA05812.1"
                     /translation="MAAPSMKWNGNLNEGAIENVSKLIKKNGIEIVDFKFNDLPGLWQ
                     HFSVPSSELSEIDDPVTSIWAEGIGFDGSSIRGFQAIQESDMILIPDATTAVMDPICE
                     VPTASIICDVYDPLTKQPYTRDPRFIAKKAEKYLKDSGIADLSYWGPELEFFIFNDIR
                     FGQSVNKGFYYIDSVEGEWNSGAVEQPNLGYKPRFKEGYFPVPPHDSLQDLRSRTILQ
                     MIRAGIPVEVHHHEVATAGQCEIDMRFDTLVQMADRCMMFKYIVKNIARKNNMVASFM
                     PKPLYGDNGSGMHTHQSLWKGGKNIFFDEKGYAKISQLAKYYAGGLLTHANALMAFCA
                     PTTNSYKRLVPGYEAPVNLVYSMRNRSAAVRIPVYSENPKTKRLEFRPPDPACNPYLA
                     FAAMLMAGLDGVKRKIDPGEPFDKNTYEVSKAEARKIPTVPGSLDKSIEALEEDHKFL
                     LEGGVFTQDVIDVWIDYKRSEMDKVRLRPHPYEFYLYFDI"
     gene            3859..5184
                     /locus_tag="VKO45_07785"
     CDS             3859..5184
                     /locus_tag="VKO45_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HMA05813.1"
                     /translation="MARDTQASILKKIKDQDIKFLRLQFCDIQGQPKNVAIPATQAEK
                     ALSQGIYFDGSSIEGFARIEESDMLLKPDPATFSVLPWRPSSSVARMICDVYTYDEKP
                     YVGDPRYILKRTLEEAARLGYVFNTGPELEFFLFKTMDGNPTIIPQDSGAYFDFAPID
                     LAENVRRDIVQALEEMGFEIEASHHEVAAGQHEIDFKYGDALRTADNVITYRIATKTI
                     ALQQGLHATFMAKPIFGINGSGMHTHCSLAKNGKNAFYDPRGDRELSPLCLHFIGGLL
                     RHVKGITRVANPTINSYKRLVPGYEAPVYISWSVTNRSALIRVPAARGNSTRAEFRSP
                     DPMCNPYLTFACMLAAGLDGIKNKIEPPASTDKNIYEMTPKERKRAKIDTLPGDLVSA
                     MTYLEKDPILTGTLGPHIMENLQRITTLEWDSFRTAVHAWEHDQYLIRY"
BASE COUNT          994 a         1716 c         1575 g          958 t
ORIGIN      
        1 cccgccccgg aacccgtcga caaccaccgc gtccgccgat gaccgggcga tccctgccgc
       61 gatggccgcc gagttgtgga ccgctgcgat ctttacaaag accggcttct tccactcggt
      121 ggcctccttg agggcccgga cgagctgctt caggtcctcg atgctgtaga tgtcatggtg
      181 cggggagggg ctgatggcat ccgacccgac gggaatcatc cgggtggagg agacatccgc
      241 gtccaccttc tcgcccggga ggtgcccacc gaggccaggc ttcgctccct ggccgatctt
      301 gatctcgatg gccgcccccc tctcgaggta gttgatatcg acgccgaacc tccccgatgc
      361 cacctggacg atcatccggt cctggtacgg gtacagggcc tggtgtaatc ccccttcacc
      421 cgttcccatg taggtccccg cctctttggc tgcccgggcg agggagagct gtgcgttccg
      481 gctgatggcc ccgtagctca tgtgggcgat catgatgggg gtctcgagct ggaggttggg
      541 cgagagctcg gtcaggagct ccacctcgcc ctggccgttc ctccggagct cgagccgcga
      601 gggcttcttc ccgatgtagg tgcgcagctc catgggctcc cggagggggt cgatggaggg
      661 gttcgtcacc tggcaggcgt cgaggaccat gcggtcgaag atgatgggga aatcggccgc
      721 attgcccatg ccggcgagga tgatcttccc ggtctttgcc tggttgtaga tatcctcccg
      781 tacctccctc gtccagatcg ggtgggtccg gtagtcgacc ggccgctccc tgaggtcgat
      841 ggcatccctc gggcacatgg cgatgcagcg atggcaggcc gtacacctcc gggagtcgat
      901 gaggagccgc ccgtcctggg accggaagac cccgtagggg cagctctcga cacaccgctc
      961 gcagagcatg cactggtccc ggtcgacggt gatccggtac cggggaggga gcgcaccgat
     1021 gctgctcatg gcgagaccct cccgatgacg gggcttcccg ccctcggcat ggagacctcg
     1081 ccgacctccg ggtccatccg gcggatggcc gcctcctcgc tcgagatgta gagccggtcg
     1141 ccattctcac cggcgaccag gggccggagc ttgatccggt cggtgaaccc gagcatcatg
     1201 tcaggtcggg cgacgacgat ggcgaagggg ccgttcatga gggccgggcc gtaggtgagc
     1261 ctgatggcgg tgtcgagctc cctctccctc cggtccatcc ggtcgatctc gtcccagaag
     1321 ggcggggcga gggcgcggac ggcgagctcc tcggagaggc cgtgcttcct gaccaggagg
     1381 tcgaagaggt acgccacgac ctcggtgtcg gtgtacatgg tgcaccggta cccatatgac
     1441 tcgatgtagc gctggttcgt cccgtaggag gtgatctccc cgttatggac caccgaccac
     1501 cccaggaggt tgaaggggtg ggcacccccc caccacccgg gggtgttcgt ggggtaccgg
     1561 ttgtgcccga gccagaggta gccctggtac tcctcgatcc ggtagaagtt cgccacatcc
     1621 tcgggccatc cggaggcctt gaagaccccc atgttcttcc ccgaggagat gaccagggcc
     1681 ccgttgcggt cggtgttgat gcgcatcacg aggtgggtga tgatatcctc ttcgggtgag
     1741 ggggaccagg acatcaggct cgcatcgggc ttgaagaagt accgccacgg cgcatggatc
     1801 ctccggaggt tcggctgctc gtaggtgggg atctgcccct cgtggacgat ccggccccac
     1861 tgctccagca ggtcgtccac gggcttcttc gtctcgtgga tgttgtcaaa gaagacatgg
     1921 agggcatagc agtcccggta ttcggggtag cacccgtaga cggcatagcc ggccccctct
     1981 ccgcttcccc gctcctgcat ctgggagagg gcctcccgga tcccggatcc gtccatgggg
     2041 ttcttcttcc ggtcgatgac cccgatgatg ccgcacatgc cgtattcccc tgagactttc
     2101 tgataattag ggtttagccg tccccccggg ctggccaccg gatggggacg ccgtacctca
     2161 gtggttggat ccctgcatag taaaatgttt ctatgttcgt ctataagatg gtatcattat
     2221 gtataaatag attgatggta gaaacatact tccatgttcg ctcagaacga tctttccgga
     2281 gggatgcggt agatggccgc gccctccatg aagtggaacg ggaacctgaa cgaaggggcc
     2341 atcgagaacg tctcgaaact tatcaagaaa aacggaatcg agatcgttga cttcaagttc
     2401 aatgacctgc ccggcctctg gcagcacttc tccgtccctt catccgagct ctccgagatc
     2461 gatgacccgg tgacctccat ctgggccgaa ggcatcggct tcgacggctc ctccatccgg
     2521 ggattccagg cgatccagga gtcggacatg atcctgatcc cggatgccac gaccgcggtc
     2581 atggacccca tctgcgaggt ccccacggcg agcatcatct gtgacgtcta cgatcccctg
     2641 accaagcagc cctacacccg ggacccgcgt ttcatcgcga agaaggccga gaagtacctg
     2701 aaggacagcg gcatcgcgga cctgagctac tggggaccgg agctcgagtt cttcatcttc
     2761 aacgacatcc ggttcggcca atcggtgaac aaggggttct actacatcga ttccgtcgag
     2821 ggagagtgga actcgggcgc cgtcgaacag cccaacctcg ggtacaagcc gaggttcaag
     2881 gaagggtact tccccgtgcc gccccatgac tccctccagg acctgcggag ccggaccatc
     2941 ctccagatga tccgggccgg gatccccgtc gaggtccacc accacgaggt ggcgaccgcg
     3001 gggcagtgcg agatcgacat gcgcttcgac accctggtgc agatggccga ccggtgcatg
     3061 atgttcaagt acatcgtgaa gaacatcgcg aggaagaaca acatggtcgc ctccttcatg
     3121 ccgaagcccc tctacggcga caatggctcg gggatgcaca cccaccagtc cctctggaag
     3181 ggcgggaaga acatcttctt cgatgagaag gggtacgcga agatcagcca gctcgccaag
     3241 tactatgccg gcgggctcct cacccacgca aacgccctca tggccttctg cgccccgacc
     3301 acgaactcct acaagcggct cgtgcccggg tacgaggcgc cggtgaacct ggtgtactcc
     3361 atgcgcaacc gctcggcggc ggtccgcatc ccggtctact cggagaaccc gaagacgaag
     3421 aggctcgagt tccggccgcc cgacccggcc tgcaacccgt acctcgcctt cgcggcgatg
     3481 ctcatggcgg gcctcgacgg ggtgaagcgg aagatcgacc ccggggagcc ctttgacaag
     3541 aacacctacg aggtctccaa ggccgaggcc cggaagatcc cgaccgtccc gggctccctc
     3601 gacaagtcca tcgaggccct ggaggaggac cacaagttcc tcctcgaggg aggggtcttt
     3661 acccaggatg tcatcgacgt ctggatcgac tacaagagat ccgagatgga caaggtccgg
     3721 ctccggcccc atccctacga gttctacctg tacttcgaca tctgatatcc ccttttttcc
     3781 ccgcgctccc tcccccatca cctgacaacc ccgtccgata cgcaagtata attcgatggg
     3841 aggtcgacct tcaccaccat ggcacgggat actcaggcat cgatcctgaa gaagatcaag
     3901 gaccaggaca tcaagttcct caggctgcag ttctgcgaca tccaggggca gccgaagaat
     3961 gtcgccatcc cggcgaccca ggccgagaag gccctctccc aggggattta cttcgacggg
     4021 tcctccatcg agggcttcgc ccggatcgag gagtccgaca tgctcctcaa gccggatccg
     4081 gccaccttct cggtcctccc ctggcggccc tcgagctcgg tcgcccggat gatctgcgac
     4141 gtgtacacct atgacgagaa gccctacgtc ggggaccccc ggtacatcct gaagcggacc
     4201 ctcgaggagg cggcgaggct ggggtacgtc ttcaacaccg gccccgagct cgagttcttc
     4261 ctcttcaaga cgatggacgg gaacccgacc atcatcccgc aggactccgg ggcctacttc
     4321 gacttcgccc ccatcgacct cgccgagaac gtgaggaggg acatcgtcca ggccctcgag
     4381 gagatgggct ttgagatcga ggcctctcac cacgaggtag ccgcaggcca gcacgagatc
     4441 gacttcaagt acggcgacgc gctgcgaacc gccgacaatg tcatcacgta ccggatcgcc
     4501 acgaagacca tcgcgctcca gcaggggctc cacgcgacct tcatggcaaa gcccattttc
     4561 gggattaacg ggagcgggat gcacacccac tgctccctcg caaagaacgg gaagaacgcc
     4621 ttttacgacc cgagggggga ccgggagctc tcgcccctgt gcctccactt catcggaggt
     4681 cttcttaggc acgtgaaggg catcacccgg gtggcgaacc cgaccatcaa ctcgtacaag
     4741 cgcctggtgc ccgggtacga ggcccccgtc tacatcagct ggtcggtcac gaaccgctcg
     4801 gccctcatcc gggtccctgc cgcgagggga aacagcaccc gggccgagtt caggtccccc
     4861 gatcccatgt gcaacccgta cctcaccttc gcctgcatgc tcgccgcagg cctggacggg
     4921 atcaagaaca agatcgagcc accggcgagc acggacaaga acatctacga aatgaccccc
     4981 aaggagcgga agagggcaaa gatcgatacc cttcccggag atctcgtctc tgccatgacg
     5041 tacctcgaaa aggatccaat cctcaccggg accctggggc cccatatcat ggagaacctg
     5101 cagagaatca cgaccctcga atgggattcc ttccggaccg ccgtgcatgc ctgggagcac
     5161 gaccagtatc tcatcagata ctagatcttt ttttttctcc accggaggac ctcccggtct
     5221 tccttcccta tttcgttccc cac
//
LOCUS       DATGDI010000210        11770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_138694, whole genome shotgun sequence.
ACCESSION   DATGDI010000210 DATGDI010000000
VERSION     DATGDI010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 11770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11770
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138694"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /gene="larC"
                     /locus_tag="VKO45_07790"
     CDS             <1..271
                     /gene="larC"
                     /locus_tag="VKO45_07790"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HMA05814.1"
                     /translation="GTLGIRVIPTVHRLVLGRRVIRVPFSVGDGTREIEVKIGSAGET
                     VTSVKPEFEQARRIAGELGVPVREVLRRAEEAAREIVSREGGRGG"
     gene            264..938
                     /gene="radB"
                     /locus_tag="VKO45_07795"
     CDS             264..938
                     /gene="radB"
                     /locus_tag="VKO45_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HMA05815.1"
                     /translation="MAERVPTGIPALDSLLGGGLERRAITQIYGEPGCGKSTLALIAA
                     AERLRAGESVIYLDTEGFSVERFRQVAGERAEELARSLFLFEPLDFEQQGLMIAESER
                     LLQKGGVGLLVIDSATALYRTELASGQEAQRRLARQVIYLLGLAKRYNVPVLITNQVY
                     TDVTRNVFMGLGGTTMAHLSKAIVRLEGSEGVRRALLEKHRSLPAGGGFSFEMTGDGI
                     RVRESP"
     gene            complement(916..1767)
                     /gene="dapF"
                     /locus_tag="VKO45_07800"
     CDS             complement(916..1767)
                     /gene="dapF"
                     /locus_tag="VKO45_07800"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HMA05816.1"
                     /translation="MAELVFTKLQGNGNDFILVDEFERELIPEELKAKFASLYAERRF
                     GIGADGVLFLSKGEKAPLKMRIFNPDESEAEMCGNGIRCFAKYVVDKGYDKYLVIKGF
                     TKDSFPVETGAGVIQVSTKYDEEEEFVATIGMPSPKFNRSEIPALGEGEYKEEILEYT
                     VYAVNTGVPHAVVIVDEVGAVEMAELAPAIRNHHTFIGGANVNFVEKSGENTLHIRTY
                     ERGVEAETLSCGTGAMAAAAVARKLGMVGDAVHVETVGGPLTVRLGEKNEMEGPARTV
                     FTGSLEL"
     gene            complement(1779..2978)
                     /locus_tag="VKO45_07805"
     CDS             complement(1779..2978)
                     /locus_tag="VKO45_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HMA05817.1"
                     /translation="MPDVIATYRFRPEEGTSPGEAARWIAEEETTGTWTDIGTRAGYV
                     RRLDGEVLEVVPGEGYYVGKIRYPAEIFEPGNIAQYLSVVAGNLFGLRRLASVRLLDI
                     DVPESLVPFSGPKFGIRGIRALIGTTNRPHVGTIIKPKVGLTPEDTAEVAYQAATGGV
                     DLIKDDETLTDQAFCPMRERVPRVMAALGKVKEETGRNVLYAVNVTTRADRIVGQAEA
                     AIALGANMVMVDVIIAGFGALQALGEAPGVTVPIHVHRAMHAAMTRNREHGVAMRPFA
                     RMVRWLGGDQLHTGTISGKMSHEAEEVRGDNAVLTGPCFGRKRVFPVSSGGLHPGKVF
                     RELEALGTDIVLQAGGGIHGHPDGTAAGAMAMRQAVDAFMEGIPLEEQAKQHYELERA
                     LKKWGSG"
     gene            complement(2983..3945)
                     /locus_tag="VKO45_07810"
     CDS             complement(2983..3945)
                     /locus_tag="VKO45_07810"
                     /EC_number="5.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0046033 - AMP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphate isomerase"
                     /protein_id="HMA05818.1"
                     /translation="MSVPSTAEKIKRMEIRGAGRIARAAVEALRSHAGEIPAKNLGEF
                     KRAMEKAGAQLLATRPTAVSLPNAVHLVMAPLAAAKNLEEARDGVIRAADRFLASSKG
                     AVERIGEIGAHHIADGETVLTHCNSEAALSCILTAARQGKDFEVYATEVRPGNQGLIT
                     LRVLSGAGIPAHYIVDSAVRHFMHRIDRVFVGADAVAVNGAVVNKIGTSQVALAAHEA
                     RVPFIVAAETYKFAPRTTLGELVEIEERAGDEVLPAAEAARLPGVRVHNPVFDVTPAS
                     YVDLIVTEEGAIPPSLAYIIIREFLGWSIEEFHKTFQIAHRDQG"
     gene            3981..4817
                     /locus_tag="VKO45_07815"
     CDS             3981..4817
                     /locus_tag="VKO45_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HMA05819.1"
                     /translation="MVAQAGSPTIAVVFDSAGTLLATYRVVKNIPTGELLPDVETTAI
                     TAASDSRALLVLNVRSRDVMKGPPGLLLSEYLGTHQVSFGVACANRVFTHEEVAEVLY
                     GDRVARVSDLQECIREVWDRCRLEALVVIDSGVILNFSSKIIEFTVTSGGRPFPGAVE
                     TVEELHRMGVAAFIASGDRASKLERIADHLMIPRDHVYGVATPSIKARIIEDLKERYD
                     LVVMVGDGINDLPAFGKADVAILTLQQPGDKPEELYRASDHVVPRVRDVVTIVRDLSR
                     RN"
     gene            4898..6517
                     /gene="atwA"
                     /locus_tag="VKO45_07820"
     CDS             4898..6517
                     /gene="atwA"
                     /locus_tag="VKO45_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase system, component
                     A2"
                     /protein_id="HMA05820.1"
                     /translation="MTPLITVDRLSMHFDGKKALNNVSFEIAEGEIMGIIGRSGAGKT
                     VLMHLMRGVDQPPTGGKVIYHVAVCDRCENIELPSMAGKPCPSCGETLAARDVDFWDE
                     RNEALKRRIMHRTAMMFQRTFALYGNDRVIENVLRALDDIDYPEEKSINRAADLLDQV
                     RLSHRMMHIARDLSGGEKQRVVLARQLAKEPFLLFADEPTGTLDPETARLVHDMLKVA
                     AKENKMGMVVTSHFSQVVQDVASRALLLNDGEIAMIGSPVEVIGKFMSGLQDLEQFTR
                     PKVGNKMIEGRDVIKRYISMDRGMVKAVNGVTFEVAEKEIFGIIGKSGAGKTTLSRMI
                     AGVMEPTTGEMNIRIGDDWVDMTKPGIEERGRAKAYIGLLHQEYDLYPHRTVLDNLTD
                     AIGLEFPKELAVRKAMITLQMAGFASEKARDILDRYPAQLSEGERHRVALAQVLIREP
                     RIVILDEPTGTMDPITKVDVKHSIMHSRDEMDMTFIVVSHDMDFVKDICDRLALMRGG
                     RIVQMGPTPKVLGSLTEEERAIMSRPAPAAE"
     gene            6522..8042
                     /locus_tag="VKO45_07825"
     CDS             6522..8042
                     /locus_tag="VKO45_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 3 protein"
                     /protein_id="HMA05821.1"
                     /translation="MEVLLDGKRRTVREGETLGAILGPRDARLAVAVIRPRAAEAAET
                     QTVQVTTTKGEVVVELLRPEPVLLAPPGKEQALALHWADRYAAAFGPFPSDLVPARSP
                     RPYERGDVLLGCGGYDNRHSYLIFSRMHHLADHGAAADGGVVGRVVSGKGVIDRWAEG
                     DRIERVERIIAWADRSTSFTTTDPGQVLEEGMQVVTRVSALARGYTPGSIDVEPAVLL
                     EHLHLAMEKGFFRVGRASSTHIRDETLLEPHIPPGAKGFRGEGTVTLRTRGPAAGAVY
                     IYRGDVAASPSHAVIGHVDHGIELVRVAGEGDLIALEINPPRIDLIGLPVREALSVAG
                     GRGLAAVADREGVGMVVVDQDPGTTLEMLAAGKVRLTTAPEEKVIGIRLFRDEASQTC
                     HLFREITGLSRHTLGRMRLFFRFEDVSLFKAELREGMRILPEHTPQGEVPAFTLGMTN
                     DSRRGAGTIGVRAASHGEFGPTSEPFEGTNIFGAVLQTEKIAGLREKEMVYVREVP"
     gene            8039..8503
                     /locus_tag="VKO45_07830"
     CDS             8039..8503
                     /locus_tag="VKO45_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 6 protein"
                     /protein_id="HMA05822.1"
                     /translation="MKSYTPAYVGTVTVYIFIESTDFTPEDIAVRAYEIAPRAMVKET
                     CFGLEVTGKPEEVDGIVEKIRAMDPAHIFVKDRGFPPGDPRRCRANLGGARPGFHGHE
                     FELTIIGHISRGLEEDLHPSGAPVPPARSRPKAPLPVEKLEKIIAGEESAHG"
     gene            8496..8945
                     /locus_tag="VKO45_07835"
     CDS             8496..8945
                     /locus_tag="VKO45_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 5 protein"
                     /protein_id="HMA05823.1"
                     /translation="MAKVFIYPTTSLILTDLVKRFGHEPLGAAIGIRERVQTAGFDSP
                     PLQITPEEPKKGLKWAAVEVPSGVRGRMAIHGPRIEEADAAIIVNDADLAFGCGGCAR
                     TNELVIYLLRTKGMPVLDLVYPTTDEEGIAFVAAIKKFLHGLGEGKP"
     gene            8942..10183
                     /locus_tag="VKO45_07840"
     CDS             8942..10183
                     /locus_tag="VKO45_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 15 protein"
                     /protein_id="HMA05824.1"
                     /translation="MTAPVRIAQLSCGPEYSGVQKEINDAANAVGAEIFYPEMALADL
                     ERDYPRFGLDIRSPDLKLAIARAKALVEGRIDADAVFIATCFRCAEGAIVRNELRRYI
                     VEKSRLPVVSYSFTERTTAGTLLTRMEALTTIARRRALLAREVQEGLTLGVDSGSSTT
                     KAVVMRDNRIIGKGWVPTIEVVKSAETAMEQALSEAGVSRKEIEATGTTGYGRHLVGT
                     QLKADLIQEELTVNSKGAVYLANHQKGPATVIDIGGMDNKAISVYDGIPGTFTMGGIC
                     AGASGRFLEMTAKRLGVDITELGPLAMKGIAKKVPMNSYCIVFGTQSLVNALAQGSTP
                     EDVAAASCHSVAEQVFEQQLQEVDIKEPVIMVGGTSLIEGLVHAMGLLLQTKILVPPN
                     SQYIGAVGSALLTSGFIREKR"
     gene            10180..10764
                     /locus_tag="VKO45_07845"
     CDS             10180..10764
                     /locus_tag="VKO45_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 17 protein"
                     /protein_id="HMA05825.1"
                     /translation="MTGIETFTHDCPEKTGGEYYERIAADVLQDLNLLRVVSKLHISV
                     DPTVPLFIVVGVIRKLPAVVKVHDFASAQVQGNRITLSIGDEAYLAPFLRILWDRYGK
                     QNVEQPDRWTVVVTVPEGGAEGFEDIPVYDPREDIFRDLMYAMVCIQPEGFKVRKEAY
                     VDGKFWFVASEDTLPEDVVEKQVREKFALIGEEL"
     gene            10761..11699
                     /locus_tag="VKO45_07850"
     CDS             10761..11699
                     /locus_tag="VKO45_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 7 protein"
                     /protein_id="HMA05826.1"
                     /translation="MILEPITYKGGLYRYEEMVELIEDLGGYIIQLQQIAGDIIILAL
                     VPKEDLELVKAVARPINGTVKVSPLVGTEIAVVSMSLEIHHLPHATCDVAEYLRQFGA
                     KTNVIGLARGFGKRIAQLNVEERDVINEHDCAIFLYGNFEECIRYKNPKISRGITVPI
                     IATGGPDREALMKCIDPPVEGYVGGLGRLAHRAKRPEEIAKLEEVVTEVSRVLDLRRT
                     EIFKDPLSVAPPRLMDLILEKVEAIHEVTSPTPVTVQMAGLRVKLPYESYAGPLRDLE
                     LEQGVKLGDIADVLPSRMRTYIWVRVRPFSETKVMV"
BASE COUNT         2015 a         4007 c         3883 g         1865 t
ORIGIN      
        1 cgggaccctc gggatccggg tcatcccaac cgtccaccgc ctcgtcctcg ggaggcgggt
       61 gatccgggtg cccttcagcg tcggcgacgg gacccgggag atcgaggtga agatcgggtc
      121 agcgggggaa acagtcacat cggtgaagcc ggagttcgag caggcccgcc gcatcgccgg
      181 ggaactcggg gtgccggtcc gggaggtgct ccgcagggcg gaggaggcgg ccagggagat
      241 cgtctcacgg gaggggggcc gtggtggctg agcgggtccc gaccgggatc cccgccctcg
      301 attccctcct gggcggaggg ctcgagcgcc gggcgatcac ccagatctat ggggagcccg
      361 gatgcgggaa gagcaccctc gccctcatcg ccgcggcgga acgcctccgg gccggcgagt
      421 cggtgatcta cctcgacacc gagggattct cggtggaaag gttccgccag gtggccgggg
      481 agcgtgccga ggaactcgcc cggagcctct tcctcttcga gcccctggac ttcgagcagc
      541 aggggctcat gatcgccgag tccgagcggc tcctccagaa gggtggagtg gggctcctcg
      601 tcatcgactc ggcgaccgcc ctctaccgca ccgagctcgc gagcgggcag gaggcacagc
      661 ggaggctcgc ccggcaggtg atctacctcc tcgggctcgc aaagcggtac aatgtccctg
      721 tcctcatcac gaaccaggtg tacacggacg tgacccggaa tgttttcatg ggcctcgggg
      781 ggacgaccat ggcccacctc tccaaggcca tcgtccggct tgaggggtcg gaaggggtac
      841 ggagggccct cctcgagaag caccggtccc tccccgcggg gggaggcttc tccttcgaga
      901 tgaccggcga cgggatcaga gttcgagaga gcccgtgaag accgtgcggg ccggcccctc
      961 catctcgttc ttctcgccga ggcggacggt gaggggaccg cccaccgtct ccacgtgcac
     1021 ggcgtccccc accatgccga gcttccgtgc cacggccgcg gcagccatgg cccccgttcc
     1081 gcaggagagg gtctcggcct cgacgccccg ctcgtaggtc cggatgtgga gggtgttctc
     1141 accgctcttc tccacgaagt tcacgttcgc cccgccgatg aaggtgtggt ggttgcggat
     1201 ggcgggggcg agctctgcca tctcgacggc cccgacctcg tccacgatga cgacggcgtg
     1261 ggggactccg gtgttcaccg cgtagacggt atattcgagg atctcctcct tgtactcccc
     1321 ctctcccagg gccgggatct cgctccggtt gaacttcggg gagggcatgc cgatggtcgc
     1381 cacgaactcc tcttcctcgt cgtacttcgt cgacacctgg atcaccccgg ccccggtctc
     1441 caccgggaag gagtccttgg tgaaaccctt gatcacgagg tacttgtcgt accccttgtc
     1501 cacgacatac ttggcgaagc accggatccc gttcccgcac atctcggcct cgctctcgtc
     1561 agggttgaag atacgcatct tcaggggggc cttctccccc ttcgagagga aaagcacccc
     1621 gtccgccccg atgccgaacc tccgttccgc atagagggaa gcgaacttcg ccttcaactc
     1681 ctcggggatc agctcgcgct cgaactcatc gacgaggata aagtcgttcc cgttcccctg
     1741 cagcttcgta aaaaccaatt ctgccatgtg atgaactcct acccgctccc ccatttcttc
     1801 agggcccgct ccagctcgta gtgctgtttc gcctgctcct cgagggggat gccctccatg
     1861 aaggcatcca ccgcctgccg catggccatc gcacccgcgg cggtcccgtc cgggtgcccg
     1921 tgaatcccgc cgccggcctg gagcacgatg tcggtcccca gggcctcgag ctcccggaag
     1981 accttccccg ggtggagccc cccgctcgaa acgggaaaga ccctctttct cccgaagcag
     2041 ggtccggtca ggaccgcgtt gtccccccgc acctcctcgg cctcgtggct catcttcccc
     2101 gagatggtgc cggtgtgcag ctggtcgccc ccgagccagc ggaccatccg ggcaaagggc
     2161 cgcatcgcaa cgccgtgctc ccggtttctc gtcatggccg catgcatcgc gcggtggaca
     2221 tggatgggga cggtgacgcc cggcgcctcc cccagggcct ggagcgcccc gaagccggcg
     2281 atgatgacat ccaccatcac catgttcgcc ccgagggcga tggccgcctc cgcctgcccc
     2341 acgatccggt cggcccgggt ggtgacgttc acggcataga gaacattccg cccggtctcc
     2401 tccttcacct tcccgagggc cgccatcact ctcgggaccc gctccctcat ggggcagaag
     2461 gcctggtcgg tgagggtctc gtcgtccttg atcaggtcca cgccgccggt ggcggcctgg
     2521 tacgccacct cggcggtatc ctccggggtg aggccgacct tgggcttgat gatggtcccg
     2581 acgtgcgggc ggttcgtggt cccgatgagg gcccggatcc cccggatccc gaactttggc
     2641 ccgctgaagg ggaccaggga ctcggggaca tcgatgtcga ggagcctgac cgaggcgagc
     2701 ctccggaggc cgaagaggtt cccggcaacc accgacagat actgggcgat gttcccgggc
     2761 tcgaagatct cggcggggta ccggatcttc ccgacgtaat atccctcccc ggggacgacc
     2821 tccagcacct cgccatccag cctccggacg taaccggccc gtgtcccgat atccgtccag
     2881 gtgccggtcg tctcctcctc ggcgatccac cgggccgcct cccccgggga ggtcccctcc
     2941 tcgggcctga acctgtaggt tgcgatgaca tccggcatgg tatcacccct gatctcgatg
     3001 ggcgatctga aatgtcttgt ggaactcctc gatggaccag cccaggaact cccggatgat
     3061 gatgtacgcg agggacgggg ggatcgcccc ctcctcggtg acgatgaggt cgacgtagga
     3121 agcgggggtc acgtcgaaga ccgggttatg gaccctgact ccgggaagcc gggcggcctc
     3181 ggcggcggga aggacctcgt ccccggcccg ttcctcgatc tccacgagct ctccaagggt
     3241 cgtccggggc gcgaacttgt aggtctcggc cgcgacgatg aaggggaccc gggcctcgtg
     3301 ggcggcgagg gcaacctgcg acgtgccgat cttgttcacc accgccccgt tcacggcgac
     3361 tgcatcggcc ccgacgaaga cccggtcgat gcggtgcatg aagtgccgga ccgccgagtc
     3421 gacgatgtag tgggcgggga tgccggcccc ggagagcacc cggagggtga tgagcccctg
     3481 gttcccgggc ctcacctcgg tcgcgtagac ctcgaagtcc ttcccctgcc gcgccgccgt
     3541 gaggatgcag gagagggcgg cctcggagtt gcagtgggtg aggaccgtct ctccgtcggc
     3601 gatgtggtgg gccccgatct ccccgatccg ctccacggcc cccttcgacg aggcgaggaa
     3661 ccggtccgcg gcccggatca cgccgtcccg ggcctcctcc aggttcttcg cggccgcgag
     3721 cggggccatg accaggtgca cggcattcgg gagggagacg gcggtgggcc gggtggcgag
     3781 gagctgggcg ccggccttct ccatcgccct cttgaactcc ccgaggtttt tcgcggggat
     3841 ctccccggca tgggacctga gggcctcgac cgcggcgcgg gcgatcctcc ccgcgccccg
     3901 gatctccatc ctctttatct tctcggcggt cgatgggaca gacatacgaa ccggtaatct
     3961 tctctctccc ggagcataga atggttgcgc aggccggctc ccccaccatc gccgtggtct
     4021 tcgacagcgc gggaaccctc ctcgccacgt accgggtcgt gaagaacatc cccaccgggg
     4081 agctcctccc ggacgtggag accacggcca tcaccgcggc ctcagacagc cgtgccctcc
     4141 tcgtgctcaa cgtccgttcc cgggacgtca tgaaggggcc ccccggcctc ctcctctccg
     4201 agtacctggg cacccaccag gtgagcttcg gggtggcctg cgcgaaccgg gtgttcaccc
     4261 acgaggaggt ggccgaggtc ctctacggcg accgggtggc gagggtctcg gacctccagg
     4321 agtgcatccg ggaggtatgg gaccggtgcc ggctcgaggc cctcgtcgtc atcgacagcg
     4381 gggtcatcct gaacttctcc tcgaagatca tcgagttcac cgtcacctcc ggcgggaggc
     4441 ccttcccggg cgccgtggaa accgtcgagg aacttcaccg gatgggcgtc gccgccttca
     4501 tcgcctcggg cgaccgggcc tcgaaactcg agcgcatcgc ggaccatctc atgatccccc
     4561 gggaccatgt ctacggcgtc gcgactccct ccatcaaggc gaggatcatc gaggacctga
     4621 aggagcggta cgatctcgtg gtcatggtgg gggacgggat caacgacctc ccggccttcg
     4681 ggaaggcgga tgtcgccatc ctcaccctcc agcagccggg cgataaaccc gaggagctgt
     4741 accgggcctc cgaccacgtg gtgccccggg tccgtgacgt ggtcacgatc gtgagggatc
     4801 tctcccgtcg gaattagccc ccggccgggc caccggccaa tataaaagat aatatgaacc
     4861 cactgtacag agtatgggtg agaggtagga gcaccccatg acccccctga tcacggtcga
     4921 tcgactctcc atgcacttcg acgggaaaaa ggccctcaac aacgtgagtt tcgagatcgc
     4981 cgagggagag atcatgggga tcatcggccg gagcggggcg gggaagaccg tgctcatgca
     5041 cctcatgagg ggcgtggacc agccacccac cggcggaaag gtcatctatc acgtggcggt
     5101 ctgcgaccgg tgcgagaaca tcgagctccc gagcatggcg ggaaagccct gcccttcgtg
     5161 cggggagacc ctcgccgcca gggacgtgga cttctgggac gagaggaacg aggccctgaa
     5221 acggaggatc atgcaccgga cggcgatgat gttccagcgg accttcgccc tctacgggaa
     5281 tgaccgggtc atcgagaacg tgctccgcgc cctcgacgac atcgactacc ccgaggagaa
     5341 gtccatcaac cgggccgccg acctcctcga ccaggtccgg ctctcccacc ggatgatgca
     5401 catcgcccgg gacctctcgg gaggggagaa gcagagggtc gtcctcgccc ggcagctcgc
     5461 gaaggagccc tttctcctct tcgccgacga gccgacgggg accctcgacc cggagaccgc
     5521 ccgtctcgtc catgacatgc tgaaggtcgc cgcgaaggag aacaagatgg gcatggtcgt
     5581 cacctcccac ttctcccagg tggtccagga cgtggcgtcc cgcgccctcc tcctcaacga
     5641 cggggagatc gccatgatcg gctcccccgt tgaggtcatc gggaagttca tgagcggcct
     5701 ccaggacctc gagcagttca cccggccgaa ggtggggaac aagatgatcg aggggaggga
     5761 cgtgatcaag cggtacatct ccatggaccg ggggatggtg aaggcggtga acggcgtcac
     5821 cttcgaggtg gccgagaagg agatcttcgg gatcatcggg aagagcggcg cggggaaaac
     5881 caccctctcc cggatgatcg ccggggtcat ggagcccacc accggggaga tgaacatccg
     5941 gatcggggac gactgggtgg acatgacgaa gcccgggatc gaggagcggg gcagggcgaa
     6001 ggcctacatc ggcctgctcc accaggagta cgacctctac ccccaccgga ccgtcctcga
     6061 caacctcacc gatgccatcg gcctcgagtt cccgaaggag ctagccgtgc ggaaggccat
     6121 gatcaccctc cagatggcgg gcttcgcgag cgagaaggca cgggacatcc ttgaccggta
     6181 ccccgcccag ctctccgagg gggagcggca ccgggtcgcc ctcgcccagg tcctcatccg
     6241 ggagccccgg atcgtcatcc tggacgagcc gacgggaacc atggatccca tcacgaaggt
     6301 cgacgtgaag cactccatca tgcactcccg ggacgagatg gacatgacct tcatcgtcgt
     6361 ctcccatgac atggacttcg tgaaggacat ctgcgaccgg ctcgccctca tgcggggcgg
     6421 ccggatcgtc cagatgggac cgacccccaa ggtcctcggg agcctcaccg aagaggaacg
     6481 cgccatcatg tcccggcccg cacccgccgc ggagtgatcc catggaggtc ctcctggacg
     6541 ggaagcgccg cacggtccgg gagggggaaa ccctcggggc catcctcggg ccccgggacg
     6601 cccgcctcgc cgtcgcggtg atccggccga gggccgccga ggccgccgag acccagaccg
     6661 tccaggtgac gacaacgaag ggtgaggtgg tggtggagct cctccgcccg gagcccgtgc
     6721 tcctcgcccc gccgggaaag gagcaggctc tcgccctcca ctgggccgac cggtacgcgg
     6781 cggccttcgg gccgttcccg tcagacctcg tcccggcccg gtccccccgg ccctacgagc
     6841 ggggggacgt tctcctcggc tgcggggggt acgacaacag gcactcgtac ctcatcttct
     6901 cccggatgca ccacctggcc gatcacggtg ccgccgccga cggaggagtc gtcgggaggg
     6961 tcgtttcggg gaagggggtc atcgaccggt gggcggaggg ggaccggatc gagagggtcg
     7021 agcggatcat cgcctgggcc gaccgctcca cctccttcac cacgaccgac cccggccagg
     7081 tcctcgagga ggggatgcag gtcgtgaccc gggtctcggc cctcgcgagg gggtacaccc
     7141 ccggaagcat cgacgtggag cccgccgtgc tcctggagca cctccacctc gccatggaaa
     7201 aggggttctt ccgggtcggc cgggcctcga gcacccacat ccgggacgag acccttctcg
     7261 agccccacat ccccccgggc gcgaagggat tccgggggga ggggacggtc accctccgca
     7321 cccggggccc ggccgcggga gcggtctaca tctaccgggg ggacgtggcc gcgagcccgt
     7381 cccacgccgt catcgggcac gtggaccatg ggatcgagct cgtccgggtt gccggggagg
     7441 gggacctcat cgccctcgag atcaatccgc cgaggatcga cctcatcggc cttccggtgc
     7501 gggaggcgct ctcggtcgcc ggcgggcgcg ggctcgcggc agtcgccgac cgggaagggg
     7561 tggggatggt ggtggtggat caggacccgg ggacgaccct ggagatgctc gccgccggga
     7621 aggtgaggct caccaccgcg ccggaggaga aggtcatcgg tatccggctc ttcagggacg
     7681 aggcctccca gacctgccac ctcttccggg agatcaccgg cctctcccgg cacaccctcg
     7741 ggaggatgag gctcttcttc cggtttgagg atgtctcgct cttcaaggcg gagctcaggg
     7801 aggggatgag gatcctcccc gagcacacac cccaggggga ggtcccggcc ttcaccctgg
     7861 ggatgacgaa cgactcccgg aggggggcgg ggactatcgg ggtgagggcc gcatcccatg
     7921 gagagttcgg cccgacctcg gagcctttcg aggggacgaa catcttcggg gccgtcctcc
     7981 agacggagaa gatcgcaggt ctcagggaga aggaaatggt ctatgtgagg gaggttccat
     8041 gaaaagctac accccggcct atgtcgggac ggtgaccgtc tatattttca tcgagtccac
     8101 ggacttcacc cccgaggaca tcgcggtccg ggcctacgag atcgcccccc gggcgatggt
     8161 caaggagacc tgcttcggtc tcgaggtgac ggggaaaccg gaggaggtgg acgggatcgt
     8221 agaaaagatc cgggccatgg acccggccca catctttgtc aaggaccgcg gattcccgcc
     8281 cggtgatccc cggcggtgcc gggcaaacct cggcggcgcc cggcccgggt tccatggcca
     8341 tgagttcgag ctcaccatca tcgggcatat ctcccggggc ctcgaggagg acctccaccc
     8401 ctcgggcgcc ccggttcccc ctgccaggtc ccggccgaag gcccccctcc cggtcgagaa
     8461 gctcgagaag atcatcgcag gggaggagtc cgcccatggc taaggtcttc atttacccga
     8521 cgacatctct catcctcacc gacctcgtga agcgcttcgg ccacgagccc ctgggtgctg
     8581 ccatcgggat ccgggagcgg gtccagaccg ccggcttcga ctccccgccc ctccagatca
     8641 cccccgagga accgaagaag ggcctgaagt gggccgcggt cgaggtcccg agcggcgtcc
     8701 ggggccgcat ggccatccat ggcccccgga tcgaggaggc cgacgcggcc atcatcgtga
     8761 acgacgccga cctcgccttc gggtgcgggg gatgcgcccg gacgaacgag ctcgtcatct
     8821 acctcctccg gacgaagggg atgcccgtcc tcgacctggt ctaccccacc accgatgagg
     8881 aagggatcgc cttcgtcgcg gctattaaga agttcctcca cggcctcggg gaggggaagc
     8941 catgaccgcc cccgtccgga tcgcccagct ctcctgcggc cccgagtaca gcggggtgca
     9001 gaaggagatc aacgatgccg caaacgcggt gggggccgag atcttctacc ccgagatggc
     9061 gctcgcggac ctcgagaggg actatccccg tttcggcctc gatatccgga gcccggacct
     9121 caagctcgcc atcgcccggg ccaaggccct cgtcgaaggc cggatcgatg cagacgcggt
     9181 cttcatcgcc acctgcttcc ggtgcgccga gggggccatc gtgagaaacg agctccggcg
     9241 ctacatcgtc gaaaagtccc gccttcccgt ggtctcgtac tccttcaccg agcggaccac
     9301 ggcagggacc ctcctcacgc ggatggaggc cctcaccacc atcgcccggc gccgggccct
     9361 cctcgccagg gaggtgcagg aggggctcac cctgggggtg gactccggct cctccaccac
     9421 gaaggcggtg gtgatgaggg acaaccggat catcgggaag ggctgggtcc ccaccataga
     9481 ggtggtgaag tctgcagaga ccgccatgga acaggccctc tccgaggccg gcgtctcaag
     9541 gaaggagata gaggccaccg ggacgaccgg gtatgggcgg cacctcgtcg gtacccagct
     9601 gaaggcagat ctcatccagg aggagctcac ggtgaactca aagggcgccg tctacctcgc
     9661 gaatcaccag aaggggccgg ccacggtcat cgacatcggg ggcatggaca acaaggcgat
     9721 atctgtgtac gacggcatcc ccgggacctt caccatgggc gggatctgcg ccggcgcgag
     9781 cggccggttc ctggagatga cggcgaaacg cctgggtgtc gacatcaccg agctcgggcc
     9841 ccttgccatg aaggggatcg caaagaaggt gcccatgaac tcctactgca tcgtcttcgg
     9901 gacccagtcc ctcgtgaacg ccctcgccca gggctcgacc cccgaagatg tcgcggcggc
     9961 ctcctgccac tcggtggccg agcaggtctt cgaacagcag ctccaggagg tggatatcaa
    10021 ggagccggtc atcatggtgg ggggcacctc cctcatcgag gggctcgtcc atgccatggg
    10081 cctcctcctc cagacaaaga tcctcgtccc cccgaactcc cagtacatcg gagccgtcgg
    10141 gtcggcgctc ctcacctcgg ggttcatccg ggagaagaga tgacgggcat cgagaccttc
    10201 acccacgatt gcccggagaa gaccggcggg gagtactacg agcgcatcgc ggcggacgtc
    10261 ctccaggact tgaacctcct ccgggtcgtc tccaagctcc acatctcggt agatcccacg
    10321 gtccccctct tcatcgtcgt gggggtgatc cggaagctcc ccgccgtcgt gaaggtccat
    10381 gacttcgcct cggcccaggt ccaggggaac cggatcaccc tctccatcgg tgacgaggcg
    10441 tacctcgcgc cctttctcag gatcctctgg gaccggtacg ggaagcagaa cgtggagcag
    10501 ccggaccggt ggaccgtggt cgtcaccgtc ccggagggag gtgccgaggg cttcgaggac
    10561 ataccggtct acgacccccg cgaggacatc ttccgggacc tcatgtacgc catggtctgc
    10621 atccagcccg agggcttcaa ggtccggaaa gaggcgtacg tcgacgggaa gttctggttc
    10681 gtcgcctccg aggacactct ccccgaggat gtcgtggaga aacaggtgag ggagaagttc
    10741 gccctcatcg gggaggagct gtgatcctcg agcccatcac ctacaagggc ggcctctacc
    10801 ggtacgagga gatggtggag ctcatcgagg acctgggggg gtacatcatc cagctccagc
    10861 agatcgcggg ggacatcatc atcctcgccc tcgtccccaa ggaggacctg gagctcgtga
    10921 aggccgtcgc aaggcccatc aacggcacgg tgaaggtctc acccctcgtg gggaccgaga
    10981 tcgccgtcgt ctccatgtcc ctcgagatcc accacctccc ccacgccacc tgcgacgtgg
    11041 cggagtacct caggcagttc ggggcgaaga cgaacgtcat cggcctcgcc cggggcttcg
    11101 ggaagcgcat cgcccagctg aacgtcgagg agcgggacgt catcaacgag catgactgcg
    11161 ccatcttcct ctacgggaac ttcgaggagt gcatccggta caagaacccg aagatcagca
    11221 ggggaatcac cgtccccatc atcgcgacgg gagggcccga tcgtgaggcc ctcatgaagt
    11281 gcatcgatcc gccggtcgaa gggtatgtcg gcggcctggg ccggctcgcc caccgggcga
    11341 agcgccccga ggagatcgcg aagctcgagg aggtggtcac cgaggtgagc cgggtcctcg
    11401 acctccgccg gaccgagatc ttcaaggacc ccctctcggt ggccccgccc cggctcatgg
    11461 acctgatcct cgagaaggtg gaggccatcc acgaggtgac ctcccccacc cccgtgaccg
    11521 tccagatggc aggcctccgg gtgaagctcc cctacgagtc gtacgcgggc cccctcaggg
    11581 atctggagct cgagcagggg gtgaagctcg gggacatcgc cgacgtcctc ccctcccgga
    11641 tgcgcaccta catctgggtc cgggtcaggc ccttctccga gacgaaggtt atggtgtaac
    11701 tcaacagggc ccgggccctg cctatcgcat tttggtataa acagaatgaa cgggccatcg
    11761 caatacgggg
//
LOCUS       DATGDI010000211        11140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_138720, whole genome shotgun sequence.
ACCESSION   DATGDI010000211 DATGDI010000000
VERSION     DATGDI010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11140
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138720"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VKO45_07855"
     CDS             complement(<1..593)
                     /locus_tag="VKO45_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HMA05827.1"
                     /translation="MTRNTLEIPRSTCRLFGTVKALSTVKRSAILVHGPKGCVYHINY
                     ILGMRGDRPGAIYTTSLGEHDVIFGAEGKLAEAIGELDRRLSPDLIFVLSCCASSIIG
                     EDVGSACRAARSRARVIGFECGGFEGEHHTAYGETLSRLAEELALPAEERRPASVNLI
                     GMLRAGPDLRELKRVLSLAGIEVLGVLPADATLRDLEG"
     gene            complement(682..1344)
                     /locus_tag="VKO45_07860"
     CDS             complement(682..1344)
                     /locus_tag="VKO45_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05828.1"
                     /translation="MIWGENMVRPVFPLAILITLAIILALVSGCTGQGGGSQPPVTTP
                     TIPTPRVTTPRMTTPLPLTTRTTVPVTTGVTPSWTPGSIVQEGAAIQIRGDIVGYKAP
                     QANYLDEIRFTVVLAPRAEPVTFEIPNTQIVFTKPGIPQYGVNYRILSGDVNNNLILE
                     EGEAFLVSIKFTSDASQNAIYAGQAFTMAIQNPPQRRITLTTEAPPTLTEEPMILATG
                     TS"
     gene            complement(1468..2130)
                     /locus_tag="VKO45_07865"
     CDS             complement(1468..2130)
                     /locus_tag="VKO45_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05829.1"
                     /translation="MTKFRGISPLSPPGRFLLVAGIAFLACLLAFSGCTSPVIPSPLP
                     ATTPPPVVTTQAPPTARPTIVVTTPTQQAYLTYSNPQYAFSISYPVGWTKQENVGANA
                     VVFTSPSTGSLSDIPATMKIAVILDNTLSLEQFKNAELAKRHALDNYNLIYDQAYKGT
                     GFSGWKVAYTANQGVLTEWVEIYAIRGSTAYTVIFSSRQDRYANFVIQSDQMFKSFQV
                     TG"
     gene            complement(2220..2690)
                     /locus_tag="VKO45_07870"
     CDS             complement(2220..2690)
                     /locus_tag="VKO45_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HMA05830.1"
                     /translation="MKFIADRMLGTLTRYLRFMGYDTLSANSLVPGNAREDTALLEIS
                     RKEDRILLTRDRELARRGGDRAVYLSSEEVMDQVRALSSQGFITPVLVMDRCSLCNTR
                     LRPATEAEIRSADYTPEEKDGLEFFLCPACQKVYWMGSHGRNLRDSLAKNLESP"
     gene            complement(2701..3756)
                     /gene="amrS"
                     /locus_tag="VKO45_07875"
     CDS             complement(2701..3756)
                     /gene="amrS"
                     /locus_tag="VKO45_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HMA05831.1"
                     /translation="MADDNAAIPEGSITHEARQYEKLEDTFVHCSVCAHRCRIKDGGH
                     GICGVRLNRGGVLYAATYGKISSEAVDPIEKKPLFHYLPGTLSYSLGSVGCNFQCEHC
                     QNWHISRATLSDGLLRSLSPEEGVARAKGTGCKSIAWTYNEPVIWHEYPRDMGTLARA
                     KGLGTVYVTNGYITEEALADIAPMLEAFRVDLKAFSEEFYRKVCKAKLQPVLDASKKA
                     RELGMHIETVTLVIPGLNDSMEEMEGLIRWVVENLGPATPMHFSRFHPDYRMQDREAT
                     PVATLEKICRRARELGIRFPYLGNVPGHPFENTYCPKCGALLIERTGYHTEFRRLKGD
                     RCGECGETIEIRQAVGP"
     gene            complement(3796..3963)
                     /locus_tag="VKO45_07880"
     CDS             complement(3796..3963)
                     /locus_tag="VKO45_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05832.1"
                     /translation="MLCCPRCGGTEIYEEIGGYAGTVYRCKKCGYRGAFIVEIEDEEA
                     RKQDEPAGRRS"
     gene            4005..4706
                     /gene="pyrH"
                     /locus_tag="VKO45_07885"
     CDS             4005..4706
                     /gene="pyrH"
                     /locus_tag="VKO45_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HMA05833.1"
                     /translation="MKRIVISLGGSVLVPSLEKNHIREYATLLRKLGKDHRIFIVVGG
                     GGEARRYIGVARRLGIDEATADELGIQVTRMNALLLAGALGDAAYPNIAESYRDAARF
                     GENGKIVVMGGVIPGQTTDAVAALLAEKVRADLIINWTSVDGIYSADPKKHPGAKKHS
                     LLTPGELQAIIRGDRLEAGSNTVFDTLAAKIVGRSGIPLVVLDGRKPKDPRKAIGEKK
                     GTLVSEGKAWKDIVF"
     gene            4790..4864
                     /locus_tag="VKO45_07890"
     tRNA            4790..4864
                     /locus_tag="VKO45_07890"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4826..4828,aa:Pro,seq:tgg)
     gene            4869..5513
                     /gene="nth"
                     /locus_tag="VKO45_07895"
     CDS             4869..5513
                     /gene="nth"
                     /locus_tag="VKO45_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HMA05834.1"
                     /translation="METTTALRVYRALATRYPEAMKGHRRGGNPFQVLITTILSAQTT
                     DRAVDGIAPALFRRYPTPEALAHAEAEEVEAIIHPTGFFHTKARHIIGSAGALVSDFG
                     GRVPATMAELLTLPGVGRKTANIVLAHAFHLSEGIAVDTHVKRIARRIGFTDETSQEK
                     IERDLTSIYPRDLWGSINSLFITHGRTLCTARKPRCPECPVREDCRYFRNLPKS"
     gene            complement(5528..6556)
                     /locus_tag="VKO45_07900"
     CDS             complement(5528..6556)
                     /locus_tag="VKO45_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HMA05835.1"
                     /translation="MISPTPLIIGLIILVALAFDFTNGFHDSANAISTVVSTKVLSPR
                     QAVLFAAVFNFVAAFAFGVAVASTISNLVKLDFVPINSIPWIHLAALIGAIGWNLMTW
                     WLGLPTSSSHAIIGGLLGAGIASSGLAAIKISTVIFVVTFMVLSPVIGMTIGFFFMVA
                     VLWLAKDIHRSRVDTYFRKLQLFSAGAYSFSHGSNDAQKTMGIITPLLFSIGYFGTGV
                     NPNLLPVPIWVILAAHAAIALGTLSGGWRIVKTMGYKITRLRPVDGFAAETASAATII
                     GASLAGIPVSTTHVITTSIMGVGTTRGTSAVKWGMARSIIWAWFLTIPISALIGFTSF
                     FLISHILP"
     gene            complement(6590..7219)
                     /locus_tag="VKO45_07905"
     CDS             complement(6590..7219)
                     /locus_tag="VKO45_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HMA05836.1"
                     /translation="MGLVEWLVPQDRIFFDLFEGLGNACLQASELLVTITDDYTDLKN
                     RTHKMEELEHKGDEISHRIYEQLNRTFITPLEPEEINRLTTALDDILDYIEGTTLMMM
                     NYGITDTDAAMKELAQLVHLSVAEIVLAVKAIRKIKDPSYVEEKCIEVNRLENLADDV
                     TAHAITELFKGGDAVTIIKLKDIYQNLESATDKCEDAANVLSDIAIRHS"
     gene            complement(7271..7342)
                     /locus_tag="VKO45_07910"
     tRNA            complement(7271..7342)
                     /locus_tag="VKO45_07910"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7308..7310),aa:Ala,seq:ggc)
     gene            7914..8582
                     /locus_tag="VKO45_07915"
     CDS             7914..8582
                     /locus_tag="VKO45_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HMA05837.1"
                     /translation="MLGILGGTSLLFSRLPELRARRIDTPFGPAGVLCGDIALLLRHQ
                     RGLPPHRINFRANMAALAILGVDRVIAIGSAGSLKREIPPGSLAIPGDFLSWGESPSI
                     HDHAIGHVLPLLDPGLAAALGKVVPGAQMGGVYAQTKGPRIETVAEVKALRQVADLVG
                     MTLASEATLACELGMPFAALCTVDNFAHGLGEAPTWEHILEASRAHGDRTGAIVEKIV
                     EVMG"
     gene            8582..9916
                     /locus_tag="VKO45_07920"
     CDS             8582..9916
                     /locus_tag="VKO45_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMA05838.1"
                     /translation="MARGDRPGFPKGRGEILIEGVRYRGKTVDLFIDGEGRIASLGKG
                     KGKRSRRDAETRIDGRGCILIPGFVNTHTHAAMTLMRGYADDMPLREWLEQRIWPLEA
                     HLTGEDVYWGTRLACLEMIRSGTVAFNDMYFFMEDAARAAREAGLRATLSYGFIDLGD
                     ADKREREIRATERFVSAVKGMGEPRIRPALGPHAVYTVSPEGLRWCGEHARDEKIGIH
                     VHLSETGQEVQDCMAAHGRSPARLLDACGCLTDQTVAAHCCWLDREECALLAARGTSA
                     SHNPTSNMKLSVHRAMPYPWMREEGVNVSLGTDGCASNNSLDMFGAMKEAALLQKFAW
                     GSETALPAPEALRMATEAGAQALGLPSGKIEAGAPADLVLVSRAGPSMTPLFSEESNL
                     VYAATGSSVDTVICDGRILMLHGEVPGEKEVLARAARTAASLVRRAGKPAGG"
     gene            9995..10146
                     /locus_tag="VKO45_07925"
     tRNA            join(9995..10032,10111..10146)
                     /locus_tag="VKO45_07925"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10029..10031,aa:Trp,seq:cca)
     gene            10340..>11140
                     /locus_tag="VKO45_07930"
     CDS             10340..>11140
                     /locus_tag="VKO45_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05839.1"
                     /translation="MKKTSIFLALIALVVLTALLAGCATQPSGGATPTPTPAVTTPAP
                     AKAVYSQAAVDLKLGMRKLWTDHAVWTRMYIIESLNNSNATGPAATRLLQNQVDIGNA
                     IKPVYGDAAGTQLTALLRQHILIAVDIINAVKAKNATAQAAAEAKWTSNADDIATFLA
                     SANPNWPKATLQTLLYKHLSTTKDELVARYTMNYTADVVAWDAVYNHILMMSDALSDG
                     ILKQYPEKFAGPAVYTQSQVDLRNGMRKLWTDHTVWTRLYIIESLNDSP"
BASE COUNT         2066 a         3446 c         3661 g         1967 t
ORIGIN      
        1 ccctccaggt cccggagggt cgcatcggca ggaagcaccc ccaggacctc gatccccgcg
       61 agagagagca cgcgtttcag ctcccggagg tcggggcccg ccctgagcat gccgatgagg
      121 ttcaccgacg cggggcgcct ctcctccgca ggcagggcga gctcctctgc gagccgggag
      181 agcgtctccc cgtaggcggt gtggtgttcc ccctcgaacc cgccgcactc aaacccgatc
      241 acccgggccc gggaccgggc ggcccggcag gcgctcccga catcctcccc gatgatggag
      301 gaggcgcagc aggagaggac gaagatgagg tcgggagaga gcctccggtc gagctccccg
      361 atggcctccg cgagctttcc ctcggccccg aagatgacgt catgctcgcc gagggaggtg
      421 gtgtagatcg cgcccgggcg gtcccctctc atcccgagga tgtagttgat gtggtacacg
      481 caacccttcg gcccgtgcac gaggatggca ctcctcttca cggtcgagag ggccttcacc
      541 gtgccgaaaa gccggcaggt gctccggggg atctcgaggg tattccgggt catggggcga
      601 ccgggtgatg ctcatcccag catggggact tccggatgaa aggtttttgc ccggggagag
      661 gcccccgggg aagaaaggga attatgacgt gccggtggcg aggatcatcg gctcttcggt
      721 cagggtggga ggggcctcgg tggtcaaggt gatccggcgc tgcgggggat tctggatggc
      781 catggtgaac gcctgcccgg catagatggc gttctgggac gcgtcggagg taaattttat
      841 acttacgaga aaggcctccc cctcctctag gatgaggtta ttgttcacgt cacccgagag
      901 gatccggtaa ttcaccccgt actggggaat ccccggcttt gtgaagacga tctgggtgtt
      961 cgggatctcg aaggtcacgg gctctgcccg gggagcgagc accaccgtga accgtatctc
     1021 atcgaggtaa tttgcctggg gggctttgta tcccacgata tcaccccgga tctggatcgc
     1081 cgccccttcc tgaacgatgc tcccgggggt ccaggacggg gtcaccccgg tggtgaccgg
     1141 gacggttgtc cttgtcgtca gagggagggg ggtcgtcatc cggggggtgg tgaccctggg
     1201 cgtcggaatg gtgggggtgg tgaccggggg ctgtgacccc ccgccctgcc ccgtgcaacc
     1261 ggagacgagg gcaaggatga tcgcgagggt gatgaggatt gcgaggggga atacaggccg
     1321 gaccatgttt tctccccaga tcatctgtcc tccccagata atatcctgct catggcagtc
     1381 tccgccggag agcagatact cctcggagag gatgaagccg gaacgtcacc ggctcggaag
     1441 aaaagagggg aaggttctcc ggcgtatcta gccggtgacc tggaaggatt tgaacatctg
     1501 gtcgctctgg atcacgaaat tggcgtaccg gtcctgccgg gacgagaaga tcacggtata
     1561 ggcggtcgat ccccgtatgg cataaatctc cacccattcg gtgaggaccc cctggtttgc
     1621 cgtgtaggcg accttccagc cggaaaagcc ggtgcccttg taggcctggt catagatcag
     1681 gttatagttg tccagggcat gcctcttcgc gagctcggcg ttcttgaact gctccaggga
     1741 gagggtgttg tccaggatca cggcgatctt catggtcgcc ggtatgtcgc ttaaggaccc
     1801 ggtggagggc gaggtgaaga cgacggcgtt cgccccgacg ttctcctgct tcgtccatcc
     1861 gacggggtag gagatggaaa atgcgtactg ggggttcgag taggtcaggt acgcctgctg
     1921 cgtcggggtg gtcacgacaa tggtcggccg ggcggtgggg ggggcctggg tcgtcaccac
     1981 ggggggcgga gtggtcgccg ggaggggcga ggggattacc ggtgaggtgc acccggagaa
     2041 ggcaaggagg caggcgagga aggcgatacc ggccacgagc aggaaccggc cgggcgggct
     2101 gagaggagaa ataccgcgga atttggtcat aaaagggaat tgtcaggcag gccattaaag
     2161 gtatgggagg gggcccctcg tgttgctccc cgtcccggga ataccggggg tgacggtgat
     2221 cagggagact ccaggttctt tgccaggctg tccctcaggt ttcgcccgtg ggatcccatc
     2281 cagtagacct tctggcaggc cgggcagagg aagaactcga ggccgtcctt ctcttcgggg
     2341 gtgtagtcgg ccgaccggat ctcggcctcg gttgccgggc gcagcctcgt gttgcagagg
     2401 gagcaccggt ccatcacgag gacaggtgtg atgaacccct gcgaggagag ggcccggacc
     2461 tggtccatga cctcctcgct cgaaaggtag acggcccggt ccccgcccct cctcgccagc
     2521 tcccggtccc gggtgaggag gatgcggtct tccttccggg aaatttcgag gagggcggtg
     2581 tcctcccggg cgttccccgg caccaggctg ttcgccgaga gggtgtcgta ccccatgaac
     2641 cggaggtacc gggtgagggt gccgagcatg cgatcggcga tgaacttcat ccgtgcctcc
     2701 tcacgggccg accgcctgcc ggatctcgat ggtctccccg cattcgccgc accggtcccc
     2761 cttcagccgg cggaactccg tgtggtaccc cgtccgctcg atgaggagcg ccccgcactt
     2821 cggacagtag gtgttctcga agggatggcc ggggacgttc cccaggtacg gaaaccggat
     2881 cccgagttcc ctcgcccgcc ggcagatctt ctcgagggtc gccaccgggg tcgcctcccg
     2941 gtcctgcatc cggtagtcgg gatggaaccg ggagaagtgc atcggtgtcg ccggccccag
     3001 gttctcaacc acccaccgga tgagcccctc catctcctcc atggagtcgt tcagccccgg
     3061 gatgaccagg gtcacggtct cgatgtgcat gccgagctcc cgggccttct tcgaggcatc
     3121 gaggaccggc tggagctttg ccttgcagac cttccggtag aactcctcgg aaaaggcctt
     3181 caggtccacc cggaaggcct cgagcatggg cgcgatatcg gcgagggcct cctcggtgat
     3241 gtacccgttc gtcacgtaca ccgtcccgag cccttttgcc cgggccaggg tccccatgtc
     3301 ccgggggtat tcgtgccaga tgaccggctc gttgtaggtc caggcgatgg atttgcaccc
     3361 ggtccccttc gcccgggcga cgccctcctc gggggagagg ctccggagga ggccgtcaga
     3421 aagagttgcc cgggagatgt gccagttctg gcagtgctcg cactggaagt tgcagccgac
     3481 gctcccgaga gagtacgaga gggttcccgg caggtagtgg aagagcggct tcttctcgat
     3541 ggggtccacc gcctcggagc tgatcttccc gtaggtcgcg gcatagagga cccctccccg
     3601 gttcagcctg accccgcaga tgccgtgacc cccgtccttg atccggcacc ggtgggcgca
     3661 gaccgagcag tggacgaagg tgtcctcgag cttctcgtac tgcctggcct cgtgcgtgat
     3721 gcttccttcg gggatggctg cgttgtcatc agccaaggga attcctctct ggaaccgata
     3781 ggggaccccg gatatttaac tcctgcgccc cgccggctca tcctgcttcc gggcctcctc
     3841 atcctcgatc tcgacgataa aggcgccccg gtacccgcac ttcttgcacc ggtagacggt
     3901 cccggcatac cctccgatct cctcgtagat ctcggttcct ccgcaccggg ggcagcagag
     3961 catacaggta tatgtggacc tgcgagagaa tgagcttgat ggccatgaag cggatcgtca
     4021 tctccctggg gggttccgtc ctcgtcccgt ccctcgaaaa gaaccatatc agggagtacg
     4081 ccaccctcct ccggaaactc gggaaggatc accggatctt tatcgtcgtg ggcgggggag
     4141 gggaggcccg ccggtacatc ggggtcgccc ggaggctcgg gatcgacgag gcgacggcgg
     4201 acgagctcgg gatccaggtc acccggatga acgccctcct ccttgccggg gccctcgggg
     4261 atgccgcgta cccgaatatc gccgagagct accgggatgc ggcccggttc ggcgagaacg
     4321 ggaagatcgt ggtgatgggc ggcgtgatcc ccgggcagac caccgatgcg gtggccgccc
     4381 tcctcgccga gaaggtccgg gcagacctca tcatcaactg gacctcggtg gacgggatct
     4441 acagcgccga cccgaagaag caccccgggg cgaagaaaca ctcgctcctc acgcccgggg
     4501 aactccaggc catcatccgg ggcgaccggc tggaggccgg gtcgaacacc gtctttgaca
     4561 ccctcgcggc gaagatcgtc gggagaagcg gcatccccct ggtcgtcctc gatgggcgga
     4621 agccgaagga cccgagaaag gccatcgggg agaagaaggg caccctcgtt tccgagggga
     4681 aggcctggaa ggacatcgtc ttctgagaaa gggtaccatg cggcacgggc cccatatccc
     4741 cagggccttc gttagatatt tctcattccc ctcccaacga tccggggaag gggtagtagg
     4801 gtagcctggt ccatcctaga gcgtttggga cgctttgacg gcagttcgaa tctgccctac
     4861 cccatcgaat ggaaaccacg acggccctcc gggtctatcg cgccctcgcg acccggtatc
     4921 cggaggcaat gaaaggacac cggaggggcg ggaatccctt ccaggtcctt atcacgacca
     4981 tcctctcggc ccagaccacc gaccgcgccg tagacgggat cgcgcccgct ctcttcaggc
     5041 ggtacccgac gccggaagcc ctcgctcacg cagaggcgga ggaggtcgag gccatcatcc
     5101 atccgaccgg tttcttccac acgaaggccc ggcacatcat cgggagtgcc ggggccctgg
     5161 tctcggactt cggagggagg gtcccggcga ccatggcgga gctcctcacc ctgccgggcg
     5221 tgggccggaa gacggcaaac atcgtgcttg cccatgcctt ccacctctcc gaggggatcg
     5281 ccgtggacac ccacgtgaag aggatcgccc ggcggatcgg gttcaccgac gagacctccc
     5341 aggagaagat cgaacgggac ctcacctcga tctatccccg ggacctctgg ggctccatca
     5401 acagcctgtt catcacccac gggcggaccc tctgcacggc gagaaagccg agatgcccgg
     5461 agtgccccgt ccgagaggac tgccggtact tcaggaacct gccgaagagc tgaatcttcc
     5521 ctctccttca cggaaggatg tgggagatca ggaagaacga tgtaaaaccg atcagggcgc
     5581 tgatggggat cgtcaggaac caggcccaga tgatgctcct cgccatcccc cacttcaccg
     5641 cgcttgtccc cctcgttgtt cccactccca tgatggaggt ggtgatgaca tgagtcgtgg
     5701 agaccgggat ccccgccagt gaggccccga taatggtcgc ggcactcgcg gtctcggcgg
     5761 caaacccgtc gacgggccgg agcctcgtga tcttgtagcc catggtcttc acgatccgcc
     5821 acccgcctga gagggttccg agggcgatgg ccgcgtgcgc cgcgaggatc acccagatag
     5881 ggaccggcag gaggttaggg ttgacccccg tcccgaaata gccgatggag aagaggagcg
     5941 gtgtgatgat gcccatggtc ttctgggcgt cgttcgatcc atggctgaag ctgtaggccc
     6001 ctgccgaaaa gagctggagt ttccggaagt aggtgtctac cctggaccgg tgaatatcct
     6061 tcgcgagcca gaggaccgct accatgaaga agaacccgat ggtcatcccg atgacgggcg
     6121 acaggaccat gaaggtcacg acgaagatca cggttgagat cttgatggcc gcaagacctg
     6181 acgaggcgat cccggctccc aaaagcccgc cgatgatggc atgggaggag gaggtcggga
     6241 ggccgagcca ccaggtcatg aggttccacc cgatggcccc gatgagggcg gcgaggtgta
     6301 tccagggaat ggagttgatg ggcacgaagt ccagcttgac caggttgctg atggtgctcg
     6361 cgacggcaac cccgaaggca aaggcagcga cgaagttgaa gaccgcggcg aagaggaccg
     6421 cctgcctggg agagaggacc ttggtcgata ccacggtgga gatggcgttt gcggagtcgt
     6481 ggaagccgtt cgtgaagtca aaggcgaggg cgacgaggat gatgaggccg atgatgaggg
     6541 gggtaggaga aatcattgca ttccaggcat tgtgggtcgc ctctcctgct cacgaatgcc
     6601 ggatggcaat gtccgagagg acgtttgccg catcctcgca cttgtccgtg gcgctctcca
     6661 ggttctggta gatgtccttc agcttgatga tggtcaccgc gtcgccgccc ttgaagagct
     6721 cggtgatggc atgggcggtg acatcgtcgg ccaggttctc cagccggttc acctcgatgc
     6781 acttctcctc gacataggag gggtccttga tcttcctgat ggcctttacg gcaaggacga
     6841 tctcggcgac cgagaggtgg acgagctggg cgagctcctt catggcggca tcggtgtcgg
     6901 tgataccgta gttcatcatc atgagggtgg tcccttcgat gtagtccagg atatcgtcga
     6961 gggccgtggt aagccggttg atctcctcgg gctcgagggg tgtgatgaag gtgcggttca
     7021 gctgctcgta gatgcggtgg gagatctcgt cccccttgtg ctcgagctcc tccatcttgt
     7081 gggtccggtt cttcaggtcc gtgtagtcgt cggtgatggt cacgagaagc tcagatgcct
     7141 ggaggcaggc gtttcccagg ccctcgaaga gatcgaagaa gatccggtcc tggggaacca
     7201 gccactcaac aagtcccacg gaaaagtacg tccccctgtg ggatgaaaaa tgcatccaaa
     7261 ttgatccgga tggaccgagc gggaattgaa cccgcggcct cacgcatgcc aagcgtgcga
     7321 tctaccactg atctatcggc ccctagtcat attgtgcgca gggattttaa aggattctgc
     7381 tgcattttcg ggggaatact ctcctccttc gccggaaggg gaaacccgta tgaggatatc
     7441 acctcgggac cggaccttgg gagagaggtg cgtgggaaag gggaattccg agaaggaggc
     7501 atcccccggg ctgctcccgg taagcagtgg gatgcagggt caccggagga tataatgtct
     7561 tttcacagca atagtataaa tacatttcta tttataaagt catgtctggg tggcttaaac
     7621 atcaaacaag ccaatacctg ccggtgcgga ggagcgtttc ccccggaccc cctcccggga
     7681 cccggcccct tccccgatac tatctcaccg tgaacgaacg ggtgatcgcc gacgttcccg
     7741 taaaaatccc gtgagcccgg cggcaccggg ccttccccga gagatgggta catacacctt
     7801 tttttaccca caggagaaac accggagcag atcaggcggg gatgtatgca gcggagggag
     7861 aggaactcga aggaagcctg gaattattcc tttcggatag gacaggagga cgaatgctcg
     7921 gaatcctagg ggggacgagc ctcctcttct cgaggcttcc ggaactccgg gcccggagga
     7981 tcgacacccc cttcggcccc gccggggttc tctgcggaga tatcgctctc ctcctccgcc
     8041 accagcgggg actcccgccc caccggatca atttccgggc caacatggct gcactcgcca
     8101 tcctcggggt cgaccgggtc atcgccatcg gctccgcggg ttccctgaag cgggagatcc
     8161 cgccggggtc ccttgccatc ccgggggatt tcctgtcctg gggagagagc ccctccatcc
     8221 atgaccatgc catcggccac gtgctccccc tcctcgatcc gggcctcgcg gcagctctcg
     8281 ggaaggtggt gcccggtgcc caaatgggag gcgtctatgc ccagacgaag ggcccgagga
     8341 tcgagaccgt cgccgaggtg aaggccctgc gacaggtcgc cgacctcgtg gggatgaccc
     8401 tcgcctccga ggcaaccctc gcctgcgagc tcgggatgcc cttcgccgcg ctctgcaccg
     8461 tggacaactt tgcccacggc ctcggggagg cccccacctg ggagcacatc ctcgaggcgt
     8521 cccgggccca cggggaccgg accggggcca tcgtggagaa gatcgtcgag gtgatgggct
     8581 gatggccagg ggcgaccgtc cggggttccc gaagggccgg ggagaaatcc tgatcgaggg
     8641 agtcaggtac cggggaaaaa cggtggacct gttcatcgac ggagagggaa ggatcgcctc
     8701 cctggggaag gggaagggaa aaaggtcccg cagggatgcc gagacgagga tcgacgggag
     8761 ggggtgcatc ctcatccccg gcttcgtaaa cacccacacc catgcggcga tgaccctcat
     8821 gcgggggtat gccgacgaca tgcccctccg ggagtggctg gagcagagga tctggcccct
     8881 cgaggcccac ctgaccgggg aggatgtcta ctgggggacc cggctcgcct gcctcgagat
     8941 gatccggtcg ggaacggtcg ccttcaatga catgtacttc ttcatggagg atgcggcgag
     9001 ggcggccagg gaggctggcc tacgggcaac cctctcctac gggttcatcg acctcgggga
     9061 tgccgataag agggagaggg agatccgggc caccgagcgg ttcgtctccg ccgtaaaggg
     9121 gatgggggag ccgcggatcc ggcctgctct cgggccccac gcggtctaca ccgtctcccc
     9181 ggagggcctc cggtggtgcg gggagcacgc ccgggacgag aagatcggga tccatgtcca
     9241 cctctccgag acagggcagg aggtccagga ctgcatggcg gcccacggga ggagtcccgc
     9301 ccgcctcctg gatgcctgcg gctgcctcac ggaccagacg gtggccgccc actgctgctg
     9361 gctcgaccgg gaggagtgcg ccctcctcgc ggcccggggg acctcggcct cccacaaccc
     9421 cacgagcaac atgaagctct cggtccaccg ggccatgccc tatccctgga tgagggagga
     9481 gggggtgaac gtctccctgg gcaccgacgg ctgcgcctcg aacaacagcc tcgacatgtt
     9541 cggggccatg aaggaggccg ccctcctcca gaagttcgcc tgggggagcg agacggctct
     9601 cccggccccc gaggcccttc gcatggccac ggaggcgggg gcccaagccc tcggccttcc
     9661 atcggggaag atagaggcgg gcgcgccggc cgacctggtc ctcgtcagcc gggccgggcc
     9721 ctcgatgaca cccctcttct ccgaggagtc gaacctggtg tacgcggcca ccggatcctc
     9781 ggtcgacacg gttatctgcg acgggaggat ccttatgctc cacggggagg tccccggcga
     9841 gaaggaggtc ctcgcccgag ctgcgaggac cgccgcctcc ctcgtccgcc gggcggggaa
     9901 accggccggc ggctgacccg ggcccgggct tcccttccac cagccctgac acaagcgtta
     9961 aattccgaaa gggagaccct gttacgtcat ccgaggggct gtggcctaga ccggaatggc
    10021 ggcgggctcc agcggtcttt gcgggtgatg aaccatgggt cttctgatat cagatgatga
    10081 cccgtggggc actgacgcat gttcggagag acccgccgat cgtgagttca aatctcacca
    10141 gccccacgtt ttcctctgga tcccgatagc atgaaccccg cagtggggat atccgtcatt
    10201 tctcatccgg acagggatac gctcttccgt ccagtgatat ttaaacaaac tggcaaattt
    10261 tgtgaatacc ggtcacgttt attaaccgat cggggcctgt tttggatatc ccccgaaccg
    10321 gggatgcgga gtttcggaaa tgaagaaaac aagcattttc cttgccctca tagcgctcgt
    10381 cgtcctcacc gccctccttg ccggatgcgc cacccagcca tccggggggg ctaccccgac
    10441 ccccacgccg gccgtgacga cccctgcacc ggcgaaagcc gtgtacagcc aggcggcagt
    10501 cgacctcaag ctcgggatgc ggaagctctg gaccgaccat gccgtctgga cgcggatgta
    10561 catcatcgag tcgctgaata actcgaacgc gacggggccc gcggcgacgc ggctcctcca
    10621 gaaccaggtg gacatcggga acgccatcaa gccggtctat ggtgacgcgg ccggcaccca
    10681 gctcaccgcc ctcctcagac agcatatcct catcgcggtg gacatcatca atgcggtgaa
    10741 ggccaagaat gccaccgccc aggccgccgc agaggcgaaa tggacgtcga acgccgacga
    10801 tatcgcgacg ttcctcgcct cggcgaaccc gaactggccc aaggcgaccc tccagaccct
    10861 cctctacaag cacctctcga ccaccaagga tgaactcgtg gccaggtaca caatgaacta
    10921 cactgccgac gtcgtggcat gggatgccgt gtacaaccac atcctcatga tgtccgatgc
    10981 cctctccgac ggcattctca agcagtaccc cgagaagttt gcaggccccg ccgtctatac
    11041 ccagtcccag gtcgacctcc ggaacggcat gaggaagctc tggacagatc acaccgtctg
    11101 gacgaggctc tacatcatcg agtccctgaa tgactcgccg
//
LOCUS       DATGDI010000212         1616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_52036, whole genome shotgun sequence.
ACCESSION   DATGDI010000212 DATGDI010000000
VERSION     DATGDI010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52036"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VKO45_07935"
     CDS             <1..684
                     /locus_tag="VKO45_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-related McrA-glycine thioamidation protein"
                     /protein_id="HMA05840.1"
                     /translation="TSGSGSGAGMRSAVVFLGPSLERGAARRILDADYRPPAARGDIT
                     RAASEGARVIGLIDGVFLQESSVGHREILAAIKAGVRVVGASSMGALRASELHTLGME
                     GVGEVFRMYRDGILVSDDEVALAFDPESCTALSEPLVNIRATVARALDEGTIDRAAGE
                     ALLAAAVSLYYPDRTYPRVIGKAQGSVDPVSLARFSRWVETGAVDLKRMDAMRALERI
                     REILRESGQ"
     gene            705..1403
                     /locus_tag="VKO45_07940"
     CDS             705..1403
                     /locus_tag="VKO45_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HMA05841.1"
                     /translation="MCGRFTIIPTIDFHERFGLPEGPAVIPRYNIAPGQDIPVVVATS
                     GGNRLATMTWGFIPPFARDPAAGRPLINARAETILEKSSFRDAVRDHRCLVPASGFYE
                     WKREGRRKVPFYLRLPAAPLFSFAGLYATLRDPAGRDHATFAIITTEPNRLVSGLHDR
                     MPAILRQETEGDWLGPGPVTPAVLEGILSPYPAEEMEAFPVSGRVNDPENDGPDLVRP
                     LPGLENMIFSSLGD"
BASE COUNT          256 a          539 c          531 g          290 t
ORIGIN      
        1 acgagcggat cggggagcgg tgcaggaatg cggagcgcgg tcgtctttct cggcccgagc
       61 cttgagcggg gggcggcccg ccgtatcctc gatgccgact accggccccc ggccgcccgg
      121 ggcgacatca cccgggccgc cagcgagggg gcccgggtca tcggcctcat agacggggtc
      181 ttcttacagg aatcatcggt gggtcaccgg gagatcctcg cggcgatcaa ggccggggtg
      241 cgggtggtgg gtgcctcctc catgggggcg cttcgggcct ccgagctcca caccctgggg
      301 atggaggggg tcggcgaggt cttccggatg taccgggacg ggatcctcgt gtcggacgac
      361 gaggtggccc tcgccttcga ccccgagtcc tgcacggccc tctccgagcc cctggtgaac
      421 atccgggcga ccgtcgcccg ggcgctcgac gaggggacca tcgaccgggc cgccggggag
      481 gcactcctcg ccgctgccgt ctcgctctac tacccggacc ggacctaccc gcgtgtgatc
      541 gggaaggcgc agggctccgt ggatccggtc tccctcgccc gcttctcccg ctgggtggag
      601 acaggggccg tggacctgaa gcggatggac gcaatgaggg cactcgagcg gatccgggag
      661 atcctccggg agtcggggca gtagccccgc ggaacattcc caccatgtgc ggccggttta
      721 ccatcattcc cacgattgac ttccacgagc ggttcggcct tccggaggga ccggccgtca
      781 tccccaggta taacatcgca ccagggcagg acatccccgt cgtcgtcgcg acatcagggg
      841 ggaaccggct ggccaccatg acctggggct ttattccccc cttcgcgagg gatcctgccg
      901 ccgggcggcc gctcatcaat gcccgggccg agacgatcct cgaaaagtcc tccttccggg
      961 acgccgtccg ggaccaccgg tgcctggtcc ccgcgagcgg gttctacgag tggaagaggg
     1021 aggggaggag gaaggtgccg ttttatctcc ggttaccggc ggcgcccctc ttctccttcg
     1081 ccggtctgta cgcaaccctc cgggaccctg ccgggaggga tcacgcgacc tttgccatca
     1141 tcaccacgga gccgaaccgg ctcgtctccg gcctccatga ccgcatgcct gccatcctcc
     1201 ggcaggagac ggagggtgac tggctcgggc ccggcccggt gacacccgcc gttctggagg
     1261 gaatcctttc cccgtacccg gcggaggaga tggaggcatt ccctgtctcg ggcagggtga
     1321 atgatccgga aaacgacggg ccggacctgg tccgtcctct cccggggctc gaaaatatga
     1381 ttttctcatc cctcggggat tgagatctcc ttcgggctcg aaaaataaat tttcctcccc
     1441 ctcggggata gggatcccct tcgggctcga aaataagatt ttctcatccc tcggggattg
     1501 agatcccctt cggactcgaa aataagattt tctcatccct cagggatcga gatcccttcg
     1561 gactcgaaaa ataggttttc ctccccctcg ggggattgaa atcccccgtt ggggct
//
LOCUS       DATGDI010000213         3195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_110536, whole genome shotgun sequence.
ACCESSION   DATGDI010000213 DATGDI010000000
VERSION     DATGDI010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110536"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..554
                     /locus_tag="VKO45_07945"
     CDS             243..554
                     /locus_tag="VKO45_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HMA05842.1"
                     /translation="MFCPKCRSLMISTGGQLKCRKCGFIRDFTEDDAPRQTKAGGGRA
                     ITIIDDEKEIATLPTLKITCPKCGHNTAFWWLRQLRAADESEVRFFRCCECGNTWRQY
                     D"
     gene            689..>3195
                     /locus_tag="VKO45_07950"
     CDS             689..>3195
                     /locus_tag="VKO45_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006487 - protein N-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HMA05843.1"
                     /translation="MAFLGKYRQILVPVLLLAFFLLALALRGLPALFTPPGGFLPIYD
                     SDTWYTLRQVEVMVHHFPQYNWFDPMTAYPTGKFIDWGPLYPALAATLCLLTGTITRS
                     GIVATSMWLGPILGACMVPAMYMLGKVTWNRRAGLVAAGLVSFAAFRLFFLASYGFVD
                     HHIAEVLTTSLFVLAYGATLAYARGHPPDLSRAVTLLPIGGLSLLSGLLLSTGMLAST
                     TTVVLVVLALGIYTVIQVTLDDLAGRPSLYLAFMHTVIFVTASLVLLGIGIHAGGLSL
                     IQYSIGHLYVFGGIIGGTWILAAVSLATKGRKVLYLAFLAGLGIAGSFLVFALPPLQA
                     MASQGIGILAGSDAYTFGIQEMQPWSLLSAWRNLNVLPILMAGGFVFLAFRLKKGRFE
                     EGVFLGVWAVFLLAMTILHSRFEYYIGVPVILLSAVCIVETWSRGSPGLRAWIAGRRA
                     GRVPTTPSGERPREIRSSRKKPAEKKRRRQNPEGPGKADSGDGKRRIAPLLAAAVLVL
                     VASGAFISLVQDIQWGMNIAYREIPGDWREALLWMDGYTPVPGVDYFGRYGPENFSYP
                     SGSYGILATWEAGHWITFFSHRIPNVNPFQDNLMGPGGGAAFFLAPREDQAATILSGL
                     GTRYVVTDVWTATEKFQSLIPWVDPSVNATPYIRYFFIPDSVDKSHLIFDDYYAEGYF
                     RSMIVRLHFLDGSMTLPTTVKYIEYTYRKVPGEGETAPMDTVGPVITRKQDLDAAAAA
                     EKAASVNADPLKGYHAAVLSEMPTLPTTEIPALSRFRLVHESPSNITWGGNFSTALPE
                     RIRYVKVFEYVPGAHIRGTGIIELPLVTNTGRTFTYR"
BASE COUNT          563 a         1034 c          896 g          702 t
ORIGIN      
        1 gttctccctc gggcttatgc ccttcaggtt ctccaccggg tcttcgcccc gttggttctc
       61 cctcgggctt atgcccttca ggttctccac cgggtcttcg ccccgttggt tctccctcgg
      121 gcttataccc ttcaggttct cccccgggcc tttgcccacc ggtttcctcc cccgggaagg
      181 ttcccgggga gtgccccggt ccataacaag ggttaatccc cgggaggtgg aggaatgtaa
      241 ggatgttctg ccccaagtgc cggagcctca tgatctcgac gggaggccag ctcaagtgca
      301 ggaagtgcgg ctttatccgc gatttcacgg aggatgatgc cccgaggcag acgaaggccg
      361 gaggggggag ggccatcacc atcatcgacg acgaaaagga gatcgccacc ctccctaccc
      421 tcaagatcac ctgcccgaag tgcgggcaca ataccgcctt ctggtggctg cgccagctcc
      481 gcgccgcaga cgagtcagag gtccgcttct tccggtgctg cgagtgcgga aacacctggc
      541 ggcagtacga ctgatacagg agctttcatc cacccgggaa tcgcgggccc cccgttacca
      601 aagggactcc cgtcttgacg accccggatt cagaagctat atcaggatgg aatgaaagac
      661 catccgggta atcctctcca tgaaaggtat ggcatttctc ggaaaatatc gccaaatcct
      721 cgttcccgtc cttcttctcg cctttttcct cctcgctctc gcattgagag gcctcccggc
      781 cctgttcaca cctcccgggg gttttctccc catctacgat tccgacacct ggtacaccct
      841 ccgtcaggtc gaggtgatgg tccaccactt cccgcagtac aactggttcg acccgatgac
      901 cgcgtatccc accgggaagt tcatcgactg gggccccctc tatccggccc ttgcggcgac
      961 cctctgcctc ctcaccggta caatcacccg gagcgggata gtcgcaacct ccatgtggct
     1021 cggccccatc ctcggggcat gcatggtccc ggcgatgtac atgctcggga aggtgacgtg
     1081 gaaccgccgg gcggggctcg ttgccgccgg cctcgtctcc tttgccgcct tccgcctgtt
     1141 cttcctcgcg agttatggat tcgtcgatca tcacattgcc gaggtgctca ccacctccct
     1201 tttcgtcctt gcctatggcg ccaccctcgc atatgcaaga ggccatcctc ccgatctctc
     1261 ccgggcggtg acgctcctgc cgattggcgg cctctccctg ctctcaggtc tccttctctc
     1321 gacgggcatg ctcgcgagca ccaccacggt ggtgctcgtg gttcttgctc tcgggatcta
     1381 cacggtgatc caggtaaccc tcgacgatct tgcgggccgc ccttccctgt accttgcctt
     1441 catgcacacc gtgatcttcg tgaccgcatc cctcgtgctt ctcggcatcg gtattcatgc
     1501 cgggggcctg tccctcatcc agtactccat cgggcacctc tatgtttttg ggggcatcat
     1561 cgggggaacc tggatccttg ccgcggtttc cctggctaca aagggcagaa aagtcctcta
     1621 cctcgccttc ctggctggcc tgggaatcgc gggatccttc ctcgtttttg cccttcctcc
     1681 tctccaggcc atggcatctc agggcatcgg gatcctcgcg gggtcagatg catacacctt
     1741 cggtatccag gagatgcagc catggtctct cctcagtgcg tggaggaacc tgaacgtcct
     1801 ccctatcctc atggccggcg ggttcgtgtt cctggcattt cgtctgaaaa aggggcgatt
     1861 cgaggagggg gtgtttctcg gggtatgggc ggtattcctc ctcgccatga ctattctgca
     1921 cagcaggttc gagtattaca tcggggtgcc ggtcatcctc ctgtccgcag tctgtatcgt
     1981 ggaaacctgg agcaggggtt ctcctggact gagggcatgg atcgcggggc gcagggcggg
     2041 gagggtcccc acgaccccat ccggtgagcg gccccgggag atccgaagct ctcgaaagaa
     2101 accggcggaa aaaaagagaa gaaggcagaa tccggaggga cctggaaagg ccgactcagg
     2161 tgatggcaaa aggcgtatag cccctctatt ggccgcggct gttctcgttc tcgttgcctc
     2221 aggtgcgttt atctccctgg ttcaggacat ccagtggggt atgaacatcg cgtaccggga
     2281 gattcccgga gattggaggg aggccctcct ctggatggat ggatataccc cggtccccgg
     2341 ggtggattat tttggcaggt acggccctga gaacttctcc tatccctcgg gatcttacgg
     2401 gattctcgcc acgtgggaag ccgggcactg gatcaccttc ttctcgcacc gcatccccaa
     2461 cgtgaacccc ttccaggata acctcatggg gcccgggggc ggtgccgcgt tcttcctcgc
     2521 cccccgtgag gaccaggcgg ccacgattct ttccggcctg ggaacaaggt atgtcgtcac
     2581 cgatgtgtgg acggccacgg aaaaatttca gagtttgatc ccatgggttg atccatcggt
     2641 caacgcaacc ccgtacatcc gttacttttt cattccggat tccgtcgata aaagccacct
     2701 tatcttcgac gactattatg ccgagggcta ttttcggtcg atgatcgtga ggctccactt
     2761 cctggacggg tcgatgaccc tccccacgac ggtaaaatac atcgaataca cctaccggaa
     2821 ggtgccggga gagggggaaa ccgcccccat ggataccgtc ggaccagtga tcacgcggaa
     2881 gcaggatctt gatgcggcgg cagcagccga gaaggccgcc tcggtgaacg ccgaccccct
     2941 gaaggggtat catgctgcgg tactctccga aatgccgacc ctccccacca cggagatccc
     3001 ggccctgtcc cgcttcaggc ttgtccatga atccccctca aacatcacat ggggcgggaa
     3061 cttcagcaca gctctcccgg agagaatccg gtacgtcaag gtcttcgagt atgtcccggg
     3121 tgcacacatc cgggggaccg ggataatcga gctgcctctt gtcaccaata ccgggcgcac
     3181 gttcacgtac cgcga
//
LOCUS       DATGDI010000214        24144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_197491, whole genome shotgun sequence.
ACCESSION   DATGDI010000214 DATGDI010000000
VERSION     DATGDI010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 24144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24144
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197491"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VKO45_07955"
     CDS             complement(<1..666)
                     /locus_tag="VKO45_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMA05844.1"
                     /translation="MIPGDLRSDFPILAEVCYLDSAATSLSPEPVLAAMLEYEHAYRA
                     NTGRGVHRLAQIASQKYWDAHERVKGFIGAKEGELIFTRNTTEAINMVAHGFPWHKGD
                     RIITTLLEHHSNLLPWMRLRDRAGIDLRLIAPAPNGTFNLDEFEEAMTPETRLVAVTH
                     ASNVLGNIVMVREIAAICRDHGALLLVDGAQSVPHIPVDVKRLGCDFLCFSGHKMLGP
                     TGTG"
     gene            complement(663..1460)
                     /gene="larE"
                     /locus_tag="VKO45_07960"
     CDS             complement(663..1460)
                     /gene="larE"
                     /locus_tag="VKO45_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HMA05845.1"
                     /translation="MSMEDKEELLVKSIRKRGSMLIAFSGGVDSSLLAGVTMEVLGDR
                     CRAVLLDSPVVPRFALQRAKETAEGLGIALEVIPVPLMDEKTFLANPADRCCFCKKAS
                     ARVLKKRAGDLGFASIADGINASDLGEHRPGILASSEEGIVHPFVEAGITKTDIRAIA
                     REKGYSFWDRPSAACLSSRIPYGDEITLIKLRMIETAEDHLRERGFIQARVRLHGPVA
                     RIEVAAREMERLWNERSGVQADIQAAGFRYVTVDLAGYRSGSMDEVL"
     gene            complement(1560..1892)
                     /locus_tag="VKO45_07965"
     CDS             complement(1560..1892)
                     /locus_tag="VKO45_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HMA05846.1"
                     /translation="MVKTPCQQIVWDVLPAIRSALAVELVKNGVAQQDVARLLEIGPP
                     AVSQYLSRKRGYRIEFEKPIVRRIEELARDLKTGKVTDVTARICAICHEIRDEPPGAC
                     VSAKESGS"
     gene            2042..3223
                     /gene="nifS"
                     /locus_tag="VKO45_07970"
     CDS             2042..3223
                     /gene="nifS"
                     /locus_tag="VKO45_07970"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HMA05847.1"
                     /translation="MAQRIVYMDHAATTFTKPEVLAEMLPYFTERFGNPSSIYSIARE
                     SRQAVEAARGKVAKALGARPEECHFTAGGSESDNWALKGVAFANRKKGKHIITSKIEH
                     HAVLHPCHWLQKEGFEVTYLPVDQYGLVNPADLETAVRKDTILVSIMFANNEIGTIEP
                     VRELGAIAHEHNIPFHTDAVQAVGNVPIDVNSLNIDLLSLAGHKFYGPKGVGALYIRK
                     GTRIDSLIHGGGQEHNRRAGTENLAGIVGIGKAIEMATVDIEGHNRKIQAMRDRLMKG
                     ILEIPHSRLNGHQEKRLPGNLNVSIEFIEGESMLLSLDEYGICGSTGSACTSGSLEPS
                     HVLLAIGLTHEVAHGSLRLTLGDANREEDVDYVLQVLPGVVDRLRAMSPLFADFKRKG
                     G"
     gene            3234..3689
                     /gene="nifU"
                     /locus_tag="VKO45_07975"
     CDS             3234..3689
                     /gene="nifU"
                     /locus_tag="VKO45_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HMA05848.1"
                     /translation="MYTEKVMEHFRHPRNMGEIPDADGIGEVGNPVCGDIMKIFLKIR
                     DNRIEDIKFQTFGCAAAIATSSMVTELAKGKTLEEAWGITNRDVADSLEGLPPIKMHC
                     SLLAEEGIHKAINDYRVKHGLPPWEEKPSHAHEHGEEPVCEPLAGAEQH"
     gene            4513..5508
                     /locus_tag="VKO45_07980"
     CDS             4513..5508
                     /locus_tag="VKO45_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA05849.1"
                     /translation="MPPFRITRASGIPLIGSLYFGIVDRGTNLLQVRPNCSCNISCPF
                     CSVDAGPGSRTRISSYEVERPYLVEWVRQVVEFKGEGVECHIDSPGEPLLYPEIAPLV
                     KDLKGIPGVEVVSMQSNGTLLTARTIKALESAGLDRLNLSIHALDPDLARTLAGVSWY
                     DVEQVKEAAERIAGSSIDLLIAPVYLPGSNDGEIPRIIDFARRIGAGKRWPPLGIQKC
                     EHYRYGRNISGMKFQNWWQFYNRSIRGWERESGMKLLLTPGDFGIEKREMLPPVFRKG
                     EKARVDIRAPGWIRGEMLGVGRNRVVSILDCPRENGAIRVEMVSAKHNIYVARSA"
     gene            complement(5522..6550)
                     /gene="hypD"
                     /locus_tag="VKO45_07985"
     CDS             complement(5522..6550)
                     /gene="hypD"
                     /locus_tag="VKO45_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HMA05850.1"
                     /translation="MPIAAEISKAIHDLADRPYSFMHICGTHEASIARSGLRSLLPPN
                     LKIVMGPGCPVCITPQGEIDGALELAGRGITLATYGDLLRVPGSRGSLESCGGDVRVV
                     QGVHKAVEIARESDRDVVFISVGFETTAPTVAAAILAGPPDNFSILSYHRLVPPAMGW
                     LLAQGEARLDGFILPGHVCTVSGFADYEQFPVPQVVAGFLPEEILLGLLMLLKQVRAG
                     EHRVENAYPRAVCREGNPKAKEAMNRVFEPCDAEWRGFPVIPGSGLRLRKEFEAFDAL
                     KKYGLTIRHVEKHTACLCDRVLRGIAQPTDCRLYGKGCTPRTPIGPCMVSHEGACRIW
                     HMYRVKGA"
     gene            complement(6666..6797)
                     /locus_tag="VKO45_07990"
     CDS             complement(6666..6797)
                     /locus_tag="VKO45_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05851.1"
                     /translation="MLLQCQRCGYQWDYKGNADWYTSCPRCKTSVKVQRRKGKKPQH"
     gene            6906..7145
                     /locus_tag="VKO45_07995"
     CDS             6906..7145
                     /locus_tag="VKO45_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05852.1"
                     /translation="MLPILEHYNETQQKIIWYLYGNLSRGQHYFKSKYIANDLGLSSK
                     EVGTNLGILSEVCDDLKITRWSYSHSTTWMVTQRS"
     gene            7302..8012
                     /locus_tag="VKO45_08000"
     CDS             7302..8012
                     /locus_tag="VKO45_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HMA05853.1"
                     /translation="MLGISTFCLHELPLPAALDILGEHTDAVEIMDDGNHYLESAEPL
                     QSHEFRYFLHSPSRGVNIASLREPIRRASVEVLTESFSVAAEVDAPVVIHPGYFAWKG
                     EREPALLQFRRSLQELKTAAADLSVTFFVENMPAWDYFLLRFPEEIPLLDGQGLALDV
                     GHARLNGCLPSFLDLPLAHAHLHDNDGKSDSHDTIGKGTIDFRPVIRAVERDHTPAIV
                     EVATLAGVLESMKVLDRV"
     gene            complement(8064..9776)
                     /locus_tag="VKO45_08005"
     CDS             complement(8064..9776)
                     /locus_tag="VKO45_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05854.1"
                     /translation="MVVRKVSTSDVPPVVPSRRDGSRGRTFAGTVLPVLGVILLAGLL
                     ACPAEAAFSMSQVRGILRIGDTITLSGLNTDNTTTFLLLTGPYLDEYGVMLTNTSALA
                     SKGYLDSVAVYPNYTWIFEWNTSLPEVRLNEGIYIVYASTVPLPRNLLLGHTYAQQTI
                     YTQGSSSPVITPNATTPIPTPVPTWTPPPAGSEVQVSAGPSNDFPGRFDGDLIVYEAQ
                     RGGGDSDIYLYNITSGNTTAVATGPAIQKSPAVFGGTVVYSAYEVHGFNRTDADLYTY
                     DVPSGVTKRISLPGDQLNPRIYGDLIAWQDESPGRSSVNVVLYDLGTQVKLKVPARTW
                     AYSPDLSGGKVIWIDDPVAPAVFLYDIGEKTVRRVTNKTGIKDTPALDGDRITWADTR
                     NDYAEIYALDIRTGTETNVTTDDSNHFTPAISGDRVVWVDFRNGNRDIYMYDLAARRE
                     TAVTTAEMDQVAPQIAGCTIAWADNRNGSFDVYYKELPGCTPSPTPAPVSLQPEVTGA
                     STPSNTPVTPASILPTARTTAAMPPLTWTPPVSTTKSPGFGIFLALGAVGILFFIGRR
                     KWIR"
     gene            9815..9886
                     /locus_tag="VKO45_08010"
     tRNA            9815..9886
                     /locus_tag="VKO45_08010"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9847..9849,aa:Thr,seq:ggt)
     gene            9982..10413
                     /locus_tag="VKO45_08015"
     CDS             9982..10413
                     /locus_tag="VKO45_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05855.1"
                     /translation="MAFDDLKEFQGRIYSGMAVGGEHLWVYPEGIWEERKVAPDRWDF
                     RFSSLKRRARSAPEGSGAPVGTGYHWFILAHQRVRKRDKDTYETLMEGIKFKVAHRRP
                     HWRFWSTEYPDHESEREILIRILEAQLEQLRSGHVPSIRDT"
     gene            10536..11276
                     /locus_tag="VKO45_08020"
     CDS             10536..11276
                     /locus_tag="VKO45_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMA05856.1"
                     /translation="MNNLKESFDTIAAGYDEDRRLIIPDFSGFYAAAVRATASPKESP
                     SVLDIGAGTGLLSEMLLEACPEARITLMDISEKMLGVARERFAGRRNVRFIVADYRYT
                     DLGGPFNVICSALSIHHLEREEKRVLYRSIFESLEEGGTFVNADEVAGETAEEHQKNL
                     AYWDDFLLSGPLGEEGAREIIRRRESLDLMEKLSVQLAWMSEAGFASVRVVYKNRAFS
                     VFTGRRYAERASPGTLTGKASWTGSCKS"
     gene            complement(11236..11922)
                     /locus_tag="VKO45_08025"
     CDS             complement(11236..11922)
                     /locus_tag="VKO45_08025"
                     /inference="COORDINATES: protein motif:HMM:NF019949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FilR1
                     domain-containing protein"
                     /protein_id="HMA05857.1"
                     /translation="MTGSTSQAIIPKIRTLERLSLIESGEKGYMLTSLGRIIAAKIED
                     FIMTMGEVMHHQDFLSTHDIEGIPEPFLYEIGDLLNSELKYDTPNDMFHVFSHFVKLL
                     KDAEYIYGISSVMSPEIAEVLRERIMAGVPIELVVNQNVAETLRQEPYGSALKTLLSF
                     ESFRIWVTDEALRVGITVTDKHLSFGLNKKEGNLYDGSVDLYSSDPKAIEWAIRLFNF
                     YKSRSTMLSL"
     gene            complement(12160..12960)
                     /locus_tag="VKO45_08030"
     CDS             complement(12160..12960)
                     /locus_tag="VKO45_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HMA05858.1"
                     /translation="MTCISCLIQEIPPSFPMVIIPFLVTFGIYNLNRKTDESEDAINH
                     EARFRFTKRYENALFGCSLATIALALIITGYSNLPSLLITLTPFVLGTLYSVRWLPSG
                     SPYRRLKEVPFAKNLIVCISWAVPGSLLPVYLSGGTPGVKTVITCLLFFSWGFLASTS
                     PDIRDRIGDAAAGVRTIPVILGEEMTRKMMTGWNLIFGAAVLFFGYLFLDLRMTLLLA
                     ATILYAQICIHLLGRGGMRYLVCDILADGQYIFFGGAIMVLVALHVPL"
     gene            13349..13792
                     /locus_tag="VKO45_08035"
     CDS             13349..13792
                     /locus_tag="VKO45_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05859.1"
                     /translation="MECESRLNTGELLFEMILASMALGPRMSTEEKILDYNQEMLRSS
                     GIFVHDLRTILLDCSRIGGRIIRIGRFIIDLETKVGDNAKCVRDEGLIKGIIQTDGTV
                     SIEYIGDKFRRKLEFVSYESIDAGMRIPLQGVSLDTAQTESRQAS"
     gene            13921..15027
                     /gene="dinB"
                     /locus_tag="VKO45_08040"
     CDS             13921..15027
                     /gene="dinB"
                     /locus_tag="VKO45_08040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HMA05860.1"
                     /translation="MEPSRIILHLDMDSFFASVEAREHPEYRGRPLVVGADPKGGKGR
                     GVVSTASYEARKFGIRSGMPISRAYHLCPDAVYLPPHFPLYLKASRGVMDILGAYSDH
                     LHQVSIDEAFLDITHAGDFGAAETLARQIKEEILGKERLTCSIGIGPSRIVAKIASDF
                     QKPDGLTVVRPGQVTGFLDPLPVRRIPGIGKKTGGMLEQMGILIIGDLRRTDIQVLQS
                     RFGKWGLHMHELASGRDVHEFREDGASRSMSRETTFERDTDDLETLTRAVEEMAADLH
                     GYLERENLFFRTLTLKIRYTGFVTKTWSQTLPHPANSLKVIRDLAKTLLSTVPGGTVR
                     LIGIRLSNLQERGPSQRQIDEFLPSESSPDPPAG"
     gene            complement(15045..17222)
                     /locus_tag="VKO45_08045"
     CDS             complement(15045..17222)
                     /locus_tag="VKO45_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMA05861.1"
                     /translation="MISLLMVDDEEELLDLARHFLKGGGIELFTSVTASGALERHRAR
                     PCDAIVCDLEMPEMDGIELLNRLRDGGDTTPFIILTGKGSEQDAMKALNLGADFYLTK
                     GGDPEILFSELARIVGFAVKTRQIEKALRESEQRYRTIFEITATGVLGSDGTILLANT
                     EAGPLLGYRPEELVGKVNWRRFIPPDELPRISELWRRHHEDPSAASLRFETRLVDRWG
                     GNRYVNIAAKLVPGTRDSVISITDITGLKKADEKLRISESLYRTIFGTTGTGTFILDE
                     TGTIVLANAETESLLGFPPRELEGRIHWSAFIPPDELPRLSEFFRLLHEDPASSTQHY
                     EARLVDRWGVTKYGIFSASLIPGSRNSVLSVIDISGRRKAEEALRESEEKYRTLIEGA
                     TDAIVVIQDGRVVFCNSRATEMWGGNPGDLIGQTFADCIAPEDIGRIQELHGKRMGGE
                     TVPRAYDAVLLRKDGSRFNAGINAGAVTYQGKPAALAFVRDVTERKHAEEDLLTAHRK
                     LNLLSSITRHDILNQLMVILGYTEILRQEVGPGPATGIITAVEDAARNIRHSVDFTKE
                     YREVGIQAPTWQDLREVIARAGGRFSLGGVKLQIDIDGVLLYADPLLERAIYNLIDNA
                     LRHGDTLTRIRFSARESASGLSVSCEDDGVGIPAKERERVFSPRFASSSGYGLFLVRE
                     ILGITGITIREVGEPGRGADFRMEVPRGAYRIVKKGSGFPPGI"
     gene            complement(17358..18455)
                     /locus_tag="VKO45_08050"
     CDS             complement(17358..18455)
                     /locus_tag="VKO45_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA05862.1"
                     /translation="MTLLKGAIAIFRRDMKKFISTPYVIIFTLFMPIMYLVIFGNAMG
                     GTITNIHIGVVQAEPYTIETPLFTNTVEALRNFRSAPDKPTIFQVTVYPSEDQAKLAL
                     ADGEISGAAIFPSTLPGDNTIRVYEDSSDYMVPTLMEGGILGAVAATGTQNPVEINKL
                     YGKIEYFQFFGVGVIVMAIFMTTMMGGAFALIRDRENGIIEGYLVTPVKRSSIILGII
                     GSGTVRAFLAGFIIVIVDLLITGIVIRTMDQFLLVLLVLAISCIGVTSLVVSVASRFN
                     TQQEYASTVAFFNLILFMTSGAFYPTIGMPDWLRWITVINPEYYTVHALRSIILRGQG
                     MNVIGTDLLALSIFSAAAIILGITTYKRSIE"
     gene            complement(18452..19453)
                     /locus_tag="VKO45_08055"
     CDS             complement(18452..19453)
                     /locus_tag="VKO45_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA05863.1"
                     /translation="MTEEQIIRLEHLTKVFHANHAVTAVDDVSFEVEKGEIFGLLGPN
                     GAGKSTLIRILTTLILPTSGTAFVGTFDVTKDPESVRSIIGVCPQNSTLDLELTAYDN
                     LDFYGRLLNVEDSLREKRIWELLKMTDLTDRAHSRVSTFSGGMRRKLEIVRAFLHRPL
                     ILFLDEPTIGLDPDSRFEVWQQIKRLNTEETTIILTTHYMDEAERLCRRIAFMDRGRL
                     IALDTPENHKRALPAGDLIDIGVDHPDDRVLSALRESGLATSVEIKEKTVRISARNGS
                     NVLPQIIGIFERFSVPMTSISIHTPTIEDVFIHLTGKKLDSGGTEQPQPAAPGRRPA"
     gene            19694..20320
                     /locus_tag="VKO45_08060"
     CDS             19694..20320
                     /locus_tag="VKO45_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HMA05864.1"
                     /translation="MLPLILDLTGRKVVIFGGGPVGARKAGFFRGECRVVVASRSFSR
                     EIQEMDVERIGMDLEHASDGDLGDLMAGAVLVVAAVKDPALNERIRGIAMASGIFCNS
                     ATGRPGDVIIPSVGKGRSHLVAVTTFGRSPAMARFVREKIGRDAEETDLMIELQERAR
                     EVLKREEPSQERRSATLWEILRDPEAWKALSRGPDKAWEYVGQRYLHG"
     gene            20313..21617
                     /gene="hemA"
                     /locus_tag="VKO45_08065"
     CDS             20313..21617
                     /gene="hemA"
                     /locus_tag="VKO45_08065"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HMA05865.1"
                     /translation="MDSQLLTPLAMASLSHHDASAVDLEKARFPDEAAFLAEARRHFR
                     GALLLQTCNRVEILVQGTGAGLSGFLSSQARGSFRILDGTGVLDHLLHLAAGIDSMIV
                     GEDQILGQLKGALALAREAGTSTPLIDLCVEKAVHVGIQVRRRTNINRGAVSIGSAAV
                     QLAEDLLGGLTDKHILVIGSGEMGMLVAQALSARGLSAIYVANRTYERAVALAAKIGG
                     RAVNLRDLYHYIRLSDVVISCTSAPHPIIHRGPLGEAVKERRWPLEDRPRPIIIIDIA
                     QPRDVEEGAGSIEGVKVFSIDDLRVVSENNLRERRAEADRARAFIAEELGQFVSLVNR
                     AVADDTLSHLYTWAEAIRVRERDRAIHRLAMAGEDPRVREVIDDLTRVMVKKLLSDAT
                     LSIRECAERGEIQAAEAMVRAITQGDGNCLREGNRGEKDETP"
     gene            complement(21690..21938)
                     /locus_tag="VKO45_08070"
     CDS             complement(21690..21938)
                     /locus_tag="VKO45_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05866.1"
                     /translation="MEKPIGKVTHFFPKIRVAVVALSDDLKQGEEIRISGAHTNFRQM
                     VTSMQIEHKTLAAAIRGQEVGLKVDQDVKVGDLVLKTG"
     gene            22350..23315
                     /gene="hemB"
                     /locus_tag="VKO45_08075"
     CDS             22350..23315
                     /gene="hemB"
                     /locus_tag="VKO45_08075"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HMA05867.1"
                     /translation="MRRYRRRIVQELFTETCVHVSDLVVPLFVDEAIHHPIPIESMPG
                     QARQPVDDLPSVAGRIRDLDLPSVLLFGIPKEKDPGASSAYAPDGVIQRSVRSMKTAS
                     PDLVVCTDVCACEYTDHGHCGIVGEKAGEQDLLNDPSLELMNKIALSHAEAGADIVAP
                     SCMLDGMVGSIRNALDDAGFEDVLIMSYSSKFASGFYGPFREAADSGYSFGDRTTYQL
                     NPSNAREAILESALDADEGADILMVKPAGYYLDILSSLREYGLPLAAFQVSGEYAMIR
                     AAAEKGWIDGRSAVMESLTCIKRAGADLIITYFAEEAAGWANEEP"
     gene            23302..>24144
                     /locus_tag="VKO45_08080"
     CDS             23302..>24144
                     /locus_tag="VKO45_08080"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HMA05868.1"
                     /translation="MKSRELYARAVQLMPGGVSSPVRAIRPYPFYTVAARGARIMTAD
                     REDLIDCCMAYGPLLLGHSHPDILAAIRNQLEKGWLYGTPTPLEPEMAGLVREDYPSM
                     EMMRFVSSGSEATMSALRAARGFTGKSDIVKVEGGFHGAHDAVLVKAGSGATTLGVPD
                     SAGVLPDLTRHTLQVPYNDPEALEGLLARNRDVAAFILEPVLGNIGPILPEKDYLSEV
                     RSITRAHDVLLIFDEVITGYRVGPGGAQGLYGIHPDLTTLGKILGGGLPIGAFGGRRE
                     IMDYI"
BASE COUNT         4694 a         7297 c         7565 g         4588 t
ORIGIN      
        1 ccccgtcccg gttggaccaa gcatcttgtg cccggagaaa caaaggaagt cgcagcccag
       61 gcgtttcacg tcaacgggga tatgggggac cgactgggcg ccatccacga gaaggagggc
      121 accgtggtcg cggcagatgg cggcgatctc gcgaaccatg acgatgttcc ccagcacgtt
      181 cgatgcatgg gtgaccgcga cgagccgggt ctccggggtc atggcttctt cgaactcatc
      241 caggttgaag gtaccgttcg gcgccggggc gatgagccgg aggtcgatcc cggcccggtc
      301 cctgaggcgc atccagggga ggaggttgct gtggtgttcg aggagggtgg ttattatccg
      361 gtctcctttg tgccacggga acccgtgggc gaccatattg atcgcctcgg tggtattccg
      421 ggtgaagatc agctcccctt cctttgcccc gatgaacccc ttcaccctct catgggcatc
      481 ccagtatttc tgagacgcaa tctgggcgag ccggtggaca cccctgccgg tgttcgcccg
      541 gtaggcatgc tcgtactcga gcatcgcggc aaggaccggc tccggggaga ggctcgtggc
      601 ggcgctgtcc aggtaacata cctcggcgag gatcggaaaa tccgacctga gatcgccggg
      661 gatcatagca cttcatccat ggaaccgctc cggtacccgg ccagatcaac ggtcacgtac
      721 ctgaacccgg ctgcctggat gtctgcctgg accccgctcc tctcgttcca gagcctctcc
      781 atctccctgg cggcgacctc tattcgggcc acgggcccat ggagccggac cctcgcctgg
      841 atgaaacccc gttccctgag atgatcctcg gcggtttcaa tcatccggag ttttattagg
      901 gtaatctcgt caccgtaggg gatacgggaa gagaggcagg cggcggaagg gcggtcccaa
      961 aaggagtacc ccttctcacg ggcaatggcc ctgatatcgg tcttggtaat ccctgcctcg
     1021 acgaacgggt ggacgatccc ctcctcgctg ctggcgagga ttccgggacg gtgttcgccg
     1081 aggtccgagg cgttgatgcc atcggcgata ctggcgaagc ccaggtctcc cgcccgcttc
     1141 ttcagaaccc gggcagaggc cttcttgcag aaacaacagc gatccgccgg gttcgcgagg
     1201 aaggttttct catccatgag gggaaccggg ataacttcga gggcgattcc gaggccctct
     1261 gcagtttcct tcgcccgttg cagggcgaac cttggaacca ccgggctgtc caggaggacg
     1321 gcacggcacc tgtcaccgag gacctccatg gtcaccccgg cgagaagcga tgaatcaact
     1381 ccgccggaaa aggcaatgag catggaacct cgctttctta ttgatttgac gaggagctcc
     1441 tccttatcct ccatgctcat gggatcatcc accctcgttc ttggagacag gcgcccttca
     1501 gttcgttaga tacagcagga cgaagtccag acatgagctt cgcccgaaaa ccggacgggc
     1561 taagagccgg attccttcgc ggaaacgcag gcacccggcg gttcgtccct tatttcgtgg
     1621 cagatggcac agatccgggc agtcacatcc gtaactttgc cggtcttgag atcacgggcg
     1681 agttcttcaa ttcttcgcac tatgggtttc tcgaactcga tccggtaccc ccgtttcctg
     1741 gagaggtact gcgagacggc aggcggaccg atctcgagga gcctggccac atcctgctgt
     1801 gcaacgccat tctttaccag ctctaccgcg agagcggatc ggatcgcagg gagtacatcc
     1861 cagacgatct gctgacaggg agtcttcacc ataggaatac ctttaaatga atcctgagat
     1921 catatattca caattgtgaa ttcgccccgt gaaagcataa aggttccctc aaatctgagg
     1981 aactcaccac tgggcttatc aagtaccgcg ggtgataact caacccactg aggaggaagc
     2041 gatggcacaa cgaatcgtgt acatggacca tgcggcgacc accttcacga aacccgaggt
     2101 gctcgccgag atgctcccgt acttcaccga acggtttggg aacccgtcct cgatctactc
     2161 catcgcacgg gagtcgaggc aggcggtgga ggcagcaagg ggaaaggtgg caaaggctct
     2221 gggcgcacgg ccggaggaat gccacttcac cgcagggggg agcgaatcgg ataactgggc
     2281 gctcaagggg gttgccttcg cgaaccggaa gaagggaaaa cacattatca cgagcaagat
     2341 cgagcaccac gcagtccttc acccctgtca ctggcttcag aaggaaggat ttgaggtcac
     2401 ctatctcccc gttgaccagt atggtctcgt gaaccctgcc gacctcgaga ccgccgtgag
     2461 gaaggatacc atcctcgtct ccatcatgtt cgcgaacaac gagatcggaa ccatcgaacc
     2521 cgtgagggag ctcggggcca tcgcccatga acacaacata cccttccata ccgatgccgt
     2581 ccaggcggtt gggaacgtcc ccatcgatgt gaacagcctg aacatcgacc tcctctccct
     2641 tgcgggtcac aagttctacg gtccgaaggg cgtgggtgcc ctctacatcc gcaaggggac
     2701 ccggatcgat tccctgatcc acggaggggg acaggagcac aaccggaggg ccgggaccga
     2761 gaacctggca gggatcgtgg gaatcggaaa agccattgag atggcgaccg tggacatcga
     2821 ggggcataac cggaagatcc aggccatgcg ggaccggctc atgaagggaa tcctcgagat
     2881 ccctcactcc cggctgaacg gtcatcagga gaaacgcctt ccggggaacc tgaacgtctc
     2941 catcgagttc atcgagggcg agtccatgct tctctccctg gacgaatatg gcatctgtgg
     3001 atccacgggt tcggcctgca cctccgggtc cctcgagccg tcccatgtcc tcctcgccat
     3061 cggcctcacc cacgaggtgg cccatgggtc gctcaggctc accctcgggg acgcgaaccg
     3121 ggaggaggat gtggactatg tcctccaggt gctgcccggc gtcgtggacc ggctcagagc
     3181 catgtcgccc ctctttgcgg acttcaagag gaagggtggt tgagatgccg cagatgtaca
     3241 ccgagaaggt gatggagcat ttccgccacc cccggaacat gggggagatc cctgacgccg
     3301 acgggatcgg ggaggtcggg aacccggtct gcggcgatat catgaagatc ttcctgaaga
     3361 tcagggataa ccggatcgag gacataaagt tccagacctt cggctgcgcc gctgccatcg
     3421 cgacctccag catggtgacc gagctcgcga aggggaagac cctggaggag gcctggggca
     3481 tcacgaaccg ggacgtggca gactccctcg agggcctccc gcccatcaag atgcactgct
     3541 ccctcctcgc cgaggaaggg atccacaaag ccatcaacga ctaccgggtg aagcacggcc
     3601 tccccccctg ggaggagaag ccgagccatg cccatgaaca cggggaagag cccgtctgcg
     3661 aacccctggc cggggcggaa cagcactgat ccgaaacgcg cgccagcgtg ctaccaggct
     3721 gaggaattga ttttacgtcg gggctcatgg cgcagggttt gaccctgaac gagcttttca
     3781 cgtcagaaac ccactgcatg tgacaccatc ccatacccag gtgcagtatc gactattatg
     3841 ttcatatgtc tccgagatgt tgtagggagg agcggggcag aatcggttcg tgagtcttga
     3901 ccattcgttc ctttcatcgt cggacgacaa tcacggggat gatgcttcga aaagcatcag
     3961 ccttcgacgt gaaaatgagg aatgaatgga gaccggatat tgatgcaacc ccaaaaccct
     4021 aaccacccca gacacctcac cacgatattt aataatactt ttgaagaaaa atggcacatt
     4081 taggtctgtt ttagccaagg gaagtggata cacttgctat cttccctacc ggggcaactg
     4141 taccatactt tacctgtcgg gaaggggtaa aaacgtttgg aagaggggag gagtggtatg
     4201 atccccgcga ctggtcagac tttcattccc attcttattc agttggatca acgattcccg
     4261 tccagcgttc acattgtgat cgggcatcat cgcgttcgca attgaatctt ctacagaaca
     4321 caattttctc ctcgttctct tcacggagaa atatttgtct gtattcacac cctttgcatg
     4381 ggttcatgcc tgagcaaccc acccatcctt cccttcatgc ctcatcgaag gaggggaaat
     4441 acgcatcctc ataatggtga tctgtcatcc ggcacctttt acccttcgcc cttctcatac
     4501 tggatagagc tcatgccccc cttccgcatc acccgggcgt ccggtatccc cctgatcggc
     4561 tccctctact tcggcatcgt cgaccggggc acaaacctcc tccaggtgag gccgaactgc
     4621 tcctgcaaca tctcctgccc cttctgctcg gtggacgccg ggcccgggtc ccggacccgg
     4681 atcagctcat acgaggtgga acgtccgtac ctcgttgaat gggtgaggca ggtggtggag
     4741 ttcaaggggg agggcgtcga gtgccatatc gactcccccg gcgagcccct cctctacccc
     4801 gaaatcgccc ccctcgtgaa ggacctgaaa ggaatccccg gggtggaggt cgtatccatg
     4861 cagtcgaatg gtaccctcct cacagcgagg accatcaaag ctctcgaatc ggcaggcctg
     4921 gaccgtttga acctctccat ccatgccctg gatcccgatc tcgcgaggac tctcgcgggg
     4981 gtttcctggt acgacgtgga gcaggtgaag gaggcggcgg agaggatcgc gggaagctct
     5041 atcgatctcc tcatcgcgcc ggtgtacctc ccggggtcga acgacgggga aatcccccgg
     5101 atcatcgact ttgcccggag gatcggggca gggaagaggt ggccgcccct tggcatccag
     5161 aagtgcgagc actaccggta tgggaggaac atcagcggga tgaagttcca gaactggtgg
     5221 cagttctaca accggtccat caggggctgg gagagggaat cggggatgaa actcctcctc
     5281 acaccggggg acttcgggat cgaaaagaga gagatgctcc cgcccgtctt caggaagggt
     5341 gagaaggccc gggtcgatat ccgggccccc gggtggatcc ggggggagat gctcggggta
     5401 gggcgcaacc gggtcgtctc tatcctggac tgcccgaggg agaatggggc catccgggtg
     5461 gagatggtct cggcaaagca caacatctac gtggcgcggt ccgcgtaggt gcgcctgggg
     5521 gtcaggctcc tttcacccgg tacatgtgcc agatccggca ggccccctcg tggctgacca
     5581 tgcatgggcc gatgggggtg cggggggtgc atcccttccc gtagagccgg cagtcggttg
     5641 gctgggcgat gccccggagg acccggtcgc agaggcatgc ggtgtgcttc tccacgtgcc
     5701 ggatggtgag gccgtacttc ttcagggcgt cgaaggcctc gaactccttc cggaggcgga
     5761 ggccggatcc ggggataacc gggaatcccc gccactcggc atcgcagggc tcgaagaccc
     5821 ggttcatcgc ttccttcgcc ttcgggttcc cctcccggca caccgccctc gggtaggcgt
     5881 tctccacccg gtgctcgccg gccctcacct gtttcaggag catgaggagc ccgaggagta
     5941 tctcctcggg gaggaacccc gccaccacct ggggcacggg gaactgctcg tagtcggcaa
     6001 agccgctcac cgtgcataca tgcccgggga ggatgaagcc gtcgagcctc gcctcaccct
     6061 gggcgagcag ccagcccatg gcgggaggca cgagccggtg gtaggagagg atggagaagt
     6121 tgtcgggggg gccggcgagg atggccgcgg cgacggtagg agcggtcgtc tcgaagccga
     6181 cggagatgaa cacgacgtca cggtcactct cccgggcgat ctccacggcc ttgtggaccc
     6241 cctggaccac ccggacgtct cccccgcagg attcgaggga accccgggac ccgggcaccc
     6301 gcagcagatc gccgtaggtc gcgagggtga tcccccggcc cgcgagctcg agggcaccat
     6361 cgatctcccc ctggggggtg atgcagaccg ggcaccccgg gcccatgacg atcttcaggt
     6421 tggggggaag gaggctccgg aggccggacc gggcgatgga ggcctcgtgg gtaccgcaga
     6481 tgtgcatgaa ggaatagggc cgatccgcga ggtcatggat ggccttcgag atctcggcgg
     6541 cgatgggcat ggactcggat tacatatctc gccgggcaac agataaggat acctcaccgg
     6601 tagggtccgg cacggggagg agacgggggc ggcctcgcgg gaagggtggc gtggaaatat
     6661 tccggttagt gctggggctt cttccccttc ctccgctgca ccttgaccga ggtcttgcac
     6721 cgggggcacg aggtgtacca gtccgcgttc cctttgtaat cccactggta cccgcatctc
     6781 tggcactgga ggagcatatt tgtatgtatg tatgtattac gggcttaata ggttgccgat
     6841 atctttatat atgtaggtat gtatgtatgt atgtaggcaa aggggtaatg gttatgcaaa
     6901 tgttcatgct cccgatcctc gaacactaca acgagacaca gcagaagatc atctggtacc
     6961 tgtacgggaa cctgtcccgc ggccagcact acttcaaatc gaaatacatc gcgaacgacc
     7021 tcggtctctc gtccaaggaa gtgggtacga acctgggcat cctctccgag gtctgcgatg
     7081 acctgaagat cacccggtgg tcctattccc acagcaccac ctggatggtc acgcagagat
     7141 cctgatccat cccgactgca cgcaccctct cgcacccatg atccgggggt taccggatcc
     7201 ctcacaactt ccacccagtc ccataccacc ccttcgccgc attttctcgg gaatccctcc
     7261 ggggcatcgt cttctttatc ccgtgcatcc aacctcttgc catgctcggg atctcgacct
     7321 tctgcctcca cgagctcccc ctccccgccg ccctcgatat cctcggagag cacaccgatg
     7381 ccgtcgagat catggacgac gggaaccatt acctggagtc ggccgagccc ctccagagcc
     7441 acgaattccg gtacttcctg cactcgccct cccggggggt gaacatcgcg agcctccggg
     7501 agcccatcag gagggcgagc gtggaggtcc tcacagagag cttctccgtc gcggccgagg
     7561 tggatgcccc cgttgtcata caccccgggt atttcgcctg gaagggggag cgggagccgg
     7621 ccctcttgca gttccgccgt tcactccagg agctgaaaac cgcagccgcg gatctctcgg
     7681 tcaccttctt cgtggagaac atgccggcct gggactactt cctcctccgc ttccccgagg
     7741 agatccccct cctggacggc caggggcttg ccctcgacgt gggccatgca aggctgaacg
     7801 gctgcctccc gtccttcctt gacctccccc tcgcccatgc ccacctccat gacaacgacg
     7861 ggaaatcgga ttcccacgac accatcggga agggcaccat cgacttccgc cccgtgatcc
     7921 gtgcggtgga gcgcgaccat accccggcca tcgtcgaggt tgccaccctc gcgggggtcc
     7981 tggagagcat gaaggtcctg gaccgggtgt gatccagacg agggcagaac taggaggggg
     8041 gacagcggga gaaaccgcgg ctttcagcgg atccacttcc tccggccgat gaaaaacagg
     8101 atcccaacag ccccaagggc gaggaagatc ccgaatccag ggctcttcgt ggtggataca
     8161 gggggcgtcc aggtcagggg gggcatggct gccgtcgtcc tggccgtagg gaggattgag
     8221 gcaggtgtca ccggcgtgtt ggagggggtt gaggcaccag tgacctccgg ttgcagggag
     8281 accggggcgg gtgtgggtga aggagtgcag ccaggaagct ccttgtaata aacgtcaaag
     8341 gacccgttcc ggttgtccgc ccaggcaatg gtgcaccccg cgatctgcgg ggcgacctgg
     8401 tccatctcgg ccgtggtcac cgcggtctcc cggcgtgccg cgagatcgta catatagata
     8461 tcccggttcc cgttccggaa atcgacccag acgacccggt cgccggagat ggcgggagtg
     8521 aagtggttcg aatcgtcagt ggtcacattg gtctcggtcc cggtcctaat atcgagggcg
     8581 tagatctccg catagtcatt cctcgtatcg gcccaggtga tccggtcccc gtcaagggcc
     8641 ggggtgtcct tgatccccgt cttgttggtt accctccgga cggtcttctc cccgatgtca
     8701 tagaggaaga cggcgggggc gacgggatcg tcgatccaga tgacctttcc ccccgagagg
     8761 tcgggcgagt atgcccaggt acgggccggg accttcagct tcacctgggt cccgaggtca
     8821 taaaggacga cgttcacact gctgcggccc ggcgactcgt cctgccatgc gatgagatcg
     8881 ccataaatcc ggggattcaa ctggtcgccg ggcaggctga tccgcttcgt gacccccgag
     8941 gggacatcgt aggtgtagag atctgcatcg gtccggttga acccgtggac ttcataagcc
     9001 gagtagacga cggttccccc gaagacggca ggtgacttct ggatggcagg ccccgtcgcc
     9061 accgcggtgg tattcccgct cgtgatattg tacaggtaga tgtcagagtc cccgcccccc
     9121 cgctgtgctt cgtacacgat gagatccccg tcgaacctgc cggggaagtc attggagggt
     9181 ccggcgctca cctgaacctc ggaaccagcg ggaggcgggg tccaggtggg aaccggggtg
     9241 gggatcgggg tggtcgcatt gggggtgatg acaggagatg agctcccctg ggtgtagatg
     9301 gtctgctggg cataggtgtg cccgagaagc aggttccggg ggagcgggac cgtcgaggca
     9361 tacacgatat agatgccctc gtttaacctg acctccggta gagaggtgtt ccactcgaag
     9421 atccatgtgt aattcgggta gaccgccacc gagtcgagat accccttgct tgcgagggcc
     9481 gaggtattcg tgagcatcac cccgtactcg tcgaggtaag gtcccgtgag gaggaggaag
     9541 gtcgtggtat tgtcggtatt caggccggac agggtaatcg tatctccgat cctgagaatg
     9601 ccccggacct gggacatcga aaaagccgcc tctgcgggac aggcgaggag ccccgcgagg
     9661 aggatcacgc cgaggaccgg taaaacggta cccgcgaagg tccggccacg ggatccgtcc
     9721 ctccgggacg ggaccaccgg tggaacgtcg ctcgtgctca ctttccttac caccatattt
     9781 taataggacc cgccaccaat aaaaaagaat agctgcctcg gtggcatagc ggtattgcgt
     9841 cgccttggta aggcgaaggt cgcgggttca attcccgccc gaggctctcg atctctcctc
     9901 tgtcgctcgc tgggtttgcg ggcggtcact ttcacctccc ctctgcccag gtttcttccc
     9961 ttttccagcc ctttccttcg tgtggcattc gacgacctga aggagttcca ggggagaatc
    10021 tattcaggca tggccgtcgg gggagagcat ctctgggtct atcccgaggg gatctgggag
    10081 gagcgcaagg tcgcacccga ccggtgggac ttcaggttct cgtcgctgaa gaggagagcc
    10141 cggagcgcac ccgagggctc gggtgcacct gtcgggacag ggtaccactg gttcatcctc
    10201 gcccaccaga gggtcaggaa acgggacaag gacacctacg agaccctgat ggagggaatc
    10261 aaattcaagg tggcacaccg acgacctcac tggcgtttct ggagtacgga atacccggac
    10321 cacgaatccg agcgggagat cctgatccgg atcctcgaag cacagctgga acagctgaga
    10381 tcaggacatg ttccgagtat ccgtgatacc tgatcccttc gtacccttcg gcctgctccg
    10441 catgtccggg agtcagatat ctggcgaggg aggacaggct tttctcctgt tccctcgtac
    10501 cggtaggcgg gagtgggaga tcgatgggga agccggtgaa caatctgaag gagtccttcg
    10561 ataccatagc agccggttat gacgaggata ggaggttgat catccccgac ttttccgggt
    10621 tttatgctgc ggcggtgaga gcaacggcat ctccgaagga gagtccctcc gtcctcgaca
    10681 ttggggccgg gacagggctc ctctcggaga tgctcctgga ggcctgcccc gaggcccgca
    10741 tcaccctgat ggacatctcg gaaaagatgc tcggggtggc gagggaacgg ttcgcaggga
    10801 ggagaaacgt caggttcatc gtcgccgatt atcgatacac ggaccttggc gggcccttta
    10861 acgtcatctg ctctgctctc tccatccacc acctggagag ggaggaaaaa cgggtacttt
    10921 accgcagcat ctttgagtcc ctcgaagaag gagggacttt tgtgaatgcc gatgaggttg
    10981 cgggtgaaac agcggaggag caccagaaga acctcgcgta ctgggacgat ttccttctat
    11041 cgggcccgct cggcgaggag ggggcccggg agatcatccg ccgccgggaa agcctcgacc
    11101 tgatggagaa gctctctgta cagctcgcgt ggatgagtga ggccgggttc gcatcagtga
    11161 gggtggtgta caagaaccgg gcgttttcgg tttttaccgg gaggaggtac gccgaaagag
    11221 cctccccagg caccctcaca gggaaagcat cgtggaccgg ctcttgtaaa agttgaagag
    11281 acgaattgcc cattcaattg ccttcgggtc gctgctgtag aggtcgaccg acccgtcgta
    11341 caggttgccc tccttcttgt tgaggccgaa ggacaggtgc ttgtcggtca ccgtgatccc
    11401 gacccggaga gcctcatcgg tcacccagat ccgaaaggat tcaaaggata ggagagtctt
    11461 gagggcgctc ccatagggtt cctgcctcag cgtctctgcc acattctggt tcaccacgag
    11521 ttctatggga accccggcca tgatgcgctc cctcaggacc tccgcgatct cggggctcat
    11581 gacggacgag atgccgtaga tgtactcggc atccttcaga agctttacga aatgggagaa
    11641 aacatggaac atatcgttcg gggtatcgta cttcagctcg gaattgagga ggtcaccgat
    11701 ctcatagaga aaaggctcgg gtattccctc gatatcatgg gtcgacagaa aatcctggtg
    11761 gtgcatgacc tctcccatgg tcatgataaa atcctcgatt tttgcggcga tgatcctccc
    11821 gagggaggtg agcatgtagc ccttctcacc cgactcgatg agggagaggc gctcgagggt
    11881 ccgaatcttg ggaatgatgg cctgggaggt acttccggtc acttcgcgga gctcggccag
    11941 ggatttaggg gcttccgaga cggaaagaag gatttggatt ttcaatcggg aggaataaat
    12001 ggattggatt gatttgtacg tcttattata gaccaccagg ggttccatca taagatcgaa
    12061 cgccttggaa agtatatata atttgaccat ccggtcccgt cgttgggaca ccgttccgga
    12121 cccttctccc ccgggttttc cgggggtctc ccggtgatct cacaggggca catgaagggc
    12181 gacgaggacc ataatggccc ctccgaagaa gatgtactgg ccgtcggcca ggatgtcaca
    12241 gaccagatac ctcatgcccc ccctgccgag caggtgaata cagatctggg catagaggat
    12301 ggtcgcagcg aggaggaggg tcatccggag atcaaggaag aggtaaccga aaaagaggac
    12361 ggcggcaccg aagatcaggt tccatccggt catcatcttc cgggtcatct cctcgccaag
    12421 gatgaccggg atcgttctca ccccagccgc ggcatctcct atccggtctc tgatgtccgg
    12481 gctggtcgat gccagaaagc cccatgagaa aaataagagg catgtgatga cggttttcac
    12541 gcccggagtg ccgccgctca ggtaaacggg gagcaggctc ccggggaccg cccatgagat
    12601 gcagacgatc aggttcttgg caaagggaac ctccttcaga cgccggtaag ggctgccgga
    12661 aggcagccag cgcacgctgt acagggtacc aaggacgaag ggggtcaggg tgataagcag
    12721 ggaagggaga ttggagtacc ctgtgatgat gagggccagt gcgatggtcg cgagagagca
    12781 cccgaaaagg gcgttctcgt accgtttcgt aaaccggaat ctggcctcat ggttgatggc
    12841 atcttcgctc tcatcggtct ttcggttgag gttatagata ccaaaggtaa cgagaaacgg
    12901 gatgatcacc atcgggaagg aaggtgggat ctcctggatg aggcaggaga tacaggtcat
    12961 ggccacgcat tccagtgcaa ggaacaggga actgaaaacg aggaaatcga gaacgtgacg
    13021 gatctttacg ggaaaaccct tgacgatccg gggtggttcg aacagctttg ttaacggaac
    13081 cttatgtaca acaccgtggg tagagaacgt catacggata aaagttgtag atgtgcttta
    13141 cattcattac cctgacgaaa gttcatcgca gcgatgaact tgaaacaacg cgatgaaccg
    13201 gaaaagtcgg gattaatata taactcgcct gataaagtta gtgatcgggg tgaagagaca
    13261 gcgagaacag aaccttctgc ccgctggcca caccactcac caattttcac ccctctgaac
    13321 tgcacgaatc acccagggga aggacattat ggagtgcgag agcagactga acacggggga
    13381 actccttttc gagatgatcc tggcctccat ggccctcggc ccccgcatga gcaccgagga
    13441 gaagatcctc gattacaacc aggagatgct caggtcctcc gggatcttcg tccacgatct
    13501 ccggaccatc ctcctggatt gcagccgaat cgggggaagg atcatccgga tcggacggtt
    13561 tatcatcgat ctcgagacaa aggtcgggga caatgcgaaa tgcgtccgcg acgagggact
    13621 catcaagggg atcatccaga cggacggaac ggtcagcatc gagtacatcg gggacaagtt
    13681 ccggagaaaa ctcgagtttg tctcgtacga gagcatcgat gccgggatga ggatccccct
    13741 gcagggcgtt tccctggaca cggcccagac agagtccagg caggcatcgt aggaaacgcc
    13801 cctcgcctcc ctccttcccc ggatccaact tctccctttc tcgaaggact gtccagaggt
    13861 ctcttccatt ttcgccccgc agaaataagc tcatgaggaa acgggagcat gaatgatggg
    13921 atggaacctt cccgcatcat cctccacctc gacatggaca gcttctttgc ctccgtcgag
    13981 gcgagagaac accccgagta ccgggggagg ccgctcgtgg tgggggcgga cccgaagggg
    14041 gggaagggtc gcggcgtggt gagcaccgcc tcgtacgagg cgaggaagtt cggcatcagg
    14101 tcgggaatgc ccatctcccg ggcctatcac ctctgccccg atgcggtgta cctcccgccc
    14161 cacttcccgc tctacctgaa ggcctcgcgg ggcgttatgg atatcctggg ggcgtactcc
    14221 gatcacctcc accaggtgag catcgacgag gccttcctgg acatcaccca tgccggggac
    14281 ttcggggccg cggaaaccct ggcccggcag atcaaggaag agattctcgg gaaggagcgt
    14341 ctcacctgct ccatcggcat cggcccatca cggatcgtcg ccaagatcgc ctctgatttc
    14401 cagaagcccg atggccttac ggtggtccgg cccgggcagg tcaccggatt cctcgatcct
    14461 ctgcctgtgc ggaggatacc gggaatcggg aaaaagacgg gcgggatgct ggagcagatg
    14521 gggatcctga tcatcgggga cctgaggcgc acggacatcc aggtcctgca gtcccggttc
    14581 ggtaaatggg gcctccacat gcacgaactc gccagcgggc gtgacgtgca cgaattccgg
    14641 gaggacggag cctcgcggtc catgagccgc gagacgacct tcgagcggga taccgacgac
    14701 ctggagaccc tcacccgggc tgtcgaggag atggctgcgg acctgcacgg ttatctggaa
    14761 cgggagaacc tgttcttccg gaccctgacc ctgaagatca ggtacacggg attcgtcacg
    14821 aaaacctggt cccagaccct gccccacccg gccaactccc tgaaggtcat tcgggacctc
    14881 gcaaaaaccc tgctttccac ggtacccggg gggactgtcc ggctcatcgg gatcaggctc
    14941 tccaacctgc aggaacgggg cccctcccag cgtcagatag acgaattcct cccttcggaa
    15001 tcctcccctg atccgcccgc cggatagtct ggtccggggg caggttatat ccctggagga
    15061 aaaccggatc ccttcttcac gatccggtat gccccccggg gaacctccat ccggaaatcc
    15121 gccccccggc ccggctcgcc cacctcgcga atggtgatcc ccgtgatgcc gaggatctcc
    15181 cggacgagga agaggccgta tcccgacgag ctcgcgaacc gcggggagaa gacgcgctcc
    15241 ctttccttgg cggggatccc caccccatcg tcctcgcagc tgacggagag gccggaagcc
    15301 gattccctgg ccgagaaccg gatcctcgtc agggtatccc cgtgcctgag cgcgttatcg
    15361 atgagattgt agatggcacg ctcgagaagg ggatcggcat agaggaggac tccgtcgatg
    15421 tcgatctgga gtttcacgcc gccgaggctg aatctcccgc cggcccgcgc gatgacctcc
    15481 ctcagatcct gccaggtggg ggcctggatc cccacctccc ggtactcctt ggtaaaatca
    15541 acggagtgcc ggatgttccg ggccgcgtcc tcgaccgcgg tgatgatccc tgtcgcaggt
    15601 ccgggcccca cctcctgcct gaggatctcg gtatacccga gaatgaccat gagctggttc
    15661 aggatgtcgt gcctggtgat ggaggagagg aggttgagct tccggtgggc tgtgaggaga
    15721 tcctcctcgg cgtgttttct ctcggtaacg tcccggacga aggcaagggc ggccggtttt
    15781 ccctggtacg tgacggcccc ggcattgatc cccgcgttga acctgctccc gtccttccgg
    15841 aggaggacgg catcgtaagc ccgggggacc gtctcccccc ccatgcgctt cccgtgcagc
    15901 tcctggattc tcccgatatc ctcgggggcg atgcaatcgg cgaaggtttg cccgatcaga
    15961 tccccggggt tcccgcccca catctccgtg gcccggctgt tgcagaagac gacccgtccg
    16021 tcctggatca cgacaatcgc atcggtggct ccttcgatca gggtccggta cttctcctcg
    16081 ctttcccgca gggcctcctc cgccttcctg cgcccggaga tgtcgatcac cgagagcacg
    16141 ctgttcctgc tccccgggat gagggaggca gagaagatcc cgtacttggt taccccccac
    16201 cgatccacca ggcgggcctc gtagtgctgg gtcgaggagg cagggtcctc gtggaggagc
    16261 cggaagaact ccgagaggcg cggaagttcg tcgggcggga tgaaggccga ccagtgtatc
    16321 ctcccctcga gttcacgggg cggaaacccg aggagggact cggtctcggc gtttgcgagg
    16381 acgatggtcc cggtctcatc gaggatgaac gtccccgtcc cggtcgtccc gaagatggtc
    16441 cggtagaggc tctccgatat ccggagcttc tcgtccgcct tcttgagccc ggtgatatcg
    16501 gtgatggata taaccgagtc ccgggttccc gggacgagct tcgcggcgat attcacgtac
    16561 cggttcccgc cccaccggtc cacgagacgg gtctcgaagc gcaaggacgc ggccgacggg
    16621 tcttcatgat gccgtctcca cagctcggag atcctcggaa gctcatcggg cgggatgaac
    16681 cgtctccagt ttaccttgcc cacgagttcc tcgggccggt acccgaggag gggccctgcc
    16741 tcggtgttcg cgaggagaat ggtcccgtcg ctcccgagaa cccccgtagc ggtgatctcg
    16801 aagatggtcc ggtacctctg ttcgctctcc cggagggcct tttcgatctg ccgggtcttc
    16861 accgcgaatc cgactatcct cgcgagctcg gaaaagagga tctccgggtc tccgcccttg
    16921 gtgagataaa agtctgcccc gaggttcagg gccttcatcg catcctgctc gctccccttc
    16981 cccgtgagaa tgatgaacgg ggtggtatcg cctccgtccc tgagccggtt caggagttca
    17041 atcccgtcca tttccggcat ctcaaggtcg cagacaatgg catcacaggg tcgggcccgg
    17101 tgcctctcca gggccccgga tgcggtaacc gaggtgaaga gctctatccc gccccctttc
    17161 aggaaatgcc gcgcaagatc gagaagttcc tcttcatcgt caaccatgag gagggagatc
    17221 atgggcacct tcggtcggcc gttcccggga ccggatgatg gggtatctga gccgggggaa
    17281 catctacctt tcttcttgaa aatgacgcct tcgccccgac ggggtgggag cagggaccgg
    17341 ggttccggtt ccccccttca ctcgatggac ctcttatagg tcgtgatccc gaggataatg
    17401 gccgctgccg agaagatgga cagggcaagc aggtcggtcc cgatgacgtt catcccctgt
    17461 cccctgagaa tgatgcttct cagcgcatgg acggtgtaat attcggggtt gatcaccgtt
    17521 atccagcgga gccaatcggg cattcctatg gtcgggtaga aggcaccgct cgtcatgaaa
    17581 aggatcaggt tgaagaatgc taccgtcgag gcatactcct gctgggtgtt gaaccgtgaa
    17641 gctaccgaga cgacgaggga agtcactccg atgcacgaga tggcgagcac gaggaggacg
    17701 agaaggaact ggtccatcgt ccggatgacg atccccgtga tgaggaggtc cacgatgacg
    17761 atgatgaagc ctgcgaggaa cgcccgcacg gtgccgctcc cgatgatccc gaggatgatg
    17821 ctggagcgct tcacgggcgt gaccaggtac ccctcgatga tcccgttctc ccggtccctg
    17881 atgagtgcga aggccccgcc catcatggtg gtcatgaaga tggccatcac gatgacaccg
    17941 acgccgaaga actggaagta ctcgatcttc ccgtagagct tgttgatctc gacggggttc
    18001 tgcgtcccgg tcgctgccac cgcccccagg atcccgcctt ccatgagggt cggcaccatg
    18061 tagtcggagc tgtcctcgta gacccggatg gtgttgtcgc ccggaagggt cgacgggaat
    18121 atagccgctc cgctaatctc gccgtccgca agcgccagtt tggcctggtc ttccgagggg
    18181 tacaccgtga cctggaaaat cgtcggcttg tcgggcgcag acctgaaatt ccttagggcc
    18241 tcgaccgtat tcgtgaagag gggcgtctct atggtatacg gctcggcctg cacgacgccg
    18301 atatggatat tggtgatggt cccccccatc gcattcccga agatgaccag gtacatgatg
    18361 ggcatgaaga gggtgaagat gatgacgtac ggcgtgctga tgaacttctt catatcacgc
    18421 cggaatatcg cgatcgcacc cttgagcagg gtcatgccgg ccgcctcccc ggggccgcgg
    18481 gctgcggctg ctccgtccca ccggagtcca gcttcttccc cgtgagatgg ataaagacgt
    18541 cctcgatggt aggggtgtgg atggagatgg aggtcatggg gaccgagaac ctctcgaaga
    18601 tgccgatgat ctgggggagc acgttgctcc cgttcctcgc cgatatgcga acggtcttct
    18661 ccttgatctc aacggaggtt gcgaggccgg actccctgag cgccgaaaga acccggtcat
    18721 cgggatgatc gaccccgatg tcgatgaggt ccccggcggg gagggcccgc ttgtggttct
    18781 cgggggtgtc gagggcgatg agcctccccc ggtccatgaa ggcgatgcgc ctgcagagcc
    18841 gctcggcctc gtccatatag tgggtggtga ggatgatggt tgtctcctcg gtgttgagcc
    18901 tcttgatctg ctgccagacc tcgaaccggc tgtcggggtc gaggccgatg gtgggctcgt
    18961 ccaggaagag gatgaggggc cggtggagga aggcccgcac gatctcgagc ttcctccgca
    19021 ttcccccgga gaaggtgctg acccttgagt gggcacggtc ggtgagatcg gtcatcttca
    19081 ggagctccca gatccgcttc tcacgcaggc tatcctccac attcaggagc cgtccgtaga
    19141 aatcgaggtt atcgtacgcg gtgagctcca ggtcgagggt gctgttctgg gggcagacgc
    19201 cgatgatgga gcgcacgctc tctggatcct tcgtcacatc aaaggtgccc acgaaggccg
    19261 tcccggaggt gggcaggatg agggtggtga ggatccgtat cagggtactc tttcccgccc
    19321 cgttggggcc gaggagcccg aagatctccc ccttctcaac ctcgaaggac acgtcgtcaa
    19381 cggcggtcac ggcgtggttg gcgtgaaaga ccttggtgag gtgttccagc ctgataatct
    19441 gctcttcggt catggttgcg ggaaggttgt cgggtttccg ggaacgggaa agaccccgat
    19501 gcctttccca gtattgttcg tcccttcttc aatagtgttt ccttcggtag gaaataaatg
    19561 atttgtcgcg attgcgacaa cataaactct gttccccggc gggatccccc ccgggtttgt
    19621 tccttatggc gggctccggt ggcgagaaca ggatggatca ccttaatttt accctgaccc
    19681 tacatctgta ccgatgctcc cccttatcct cgatctcacc gggaggaagg tcgtgatctt
    19741 cgggggaggc ccggtcgggg cgaggaaggc gggattcttc cggggtgagt gccgggtggt
    19801 ggtggccagc cgatcctttt cccgcgagat ccaggagatg gacgtggaac gaatcgggat
    19861 ggatctcgag catgcctctg acggcgacct cggggatctc atggccggag cggtcctggt
    19921 cgtggccgcg gtgaaggacc ccgccctgaa tgaacggatc cgggggatcg ccatggcatc
    19981 aggtatcttc tgcaacagcg ccacgggccg gcccggcgat gtcatcatcc cgtcggtcgg
    20041 gaaggggcgt tcccacctcg ttgcggtgac caccttcggc cggtcgcctg ccatggcccg
    20101 gttcgtccgc gagaagatcg gccgggatgc ggaggaaacg gacctgatga tcgagctcca
    20161 ggagcgggcc cgggaggtcc tgaagagaga ggaaccctcc caggagcggc gttccgccac
    20221 tctctgggag atcctccggg acccggaggc atggaaggcc ctttcccggg ggccggataa
    20281 ggcctgggag tacgtcggtc agaggtacct ccatggatag ccagcttctc accccgctcg
    20341 ccatggcaag cctttcccac catgacgcat cggcggtgga cctggagaag gcgcggtttc
    20401 ccgacgaggc cgccttcctc gcggaggcaa ggaggcattt ccgcggggcc ctcctcctcc
    20461 agacctgcaa ccgggtggag attctcgtcc agggcacggg ggccggcctt tcggggttcc
    20521 tctcctccca ggcaaggggg agcttccgga tcctcgacgg gaccggggtc ctcgatcacc
    20581 tcctccacct cgcggcgggg atcgactcga tgatcgtggg ggaggaccag atcctcggcc
    20641 agctgaaggg tgccctcgcc ctggcaaggg aggccgggac ctccaccccc ctcatcgacc
    20701 tgtgcgtgga gaaggcagtc cacgtcggga tacaggtccg gcggaggacg aacatcaacc
    20761 ggggagcggt ctccatcggc tcagcggccg tccagctcgc ggaggacctc ctcgggggtc
    20821 ttaccgacaa gcacatcctc gtcatcggga gcggcgagat gggaatgctc gtcgcccagg
    20881 ccctctcggc ccgggggctc tcggccatct acgtggcgaa ccggacctac gaaagggcgg
    20941 tggccctggc ggcaaagata ggcggcaggg cggtgaacct gagggacctg taccactaca
    21001 tcaggctctc ggacgtggtc atctcctgca cctcggcccc ccaccccatc atccaccgcg
    21061 gaccgctcgg ggaggccgtg aaggagcggc gctggcccct cgaggaccgc ccccggccca
    21121 tcatcatcat cgacatcgcc cagccccggg acgtcgagga gggggcgggg tccatcgagg
    21181 gggtgaaggt cttctccatc gacgatctcc gggtcgtgtc cgagaacaac ctgcgggagc
    21241 ggagggccga ggcggaccgg gcccgggcct tcatcgcgga ggagctcggg cagtttgtct
    21301 ccctcgtgaa ccgggccgtc gcagatgata ccctctccca cctctacacc tgggccgagg
    21361 ccatccgggt gagggagagg gaccgggcca tccaccggct cgcgatggca ggggaagacc
    21421 cccgggtgcg ggaggtcatc gacgacctca cgcgggtgat ggtgaagaag ctcctctcgg
    21481 atgccaccct ctccatccgt gagtgcgccg agcgcgggga gatccaggcc gcggaggcca
    21541 tggtgcgggc catcacccag ggtgacggga actgcctgcg ggaagggaac cggggggaga
    21601 aggacgagac accctgaccg gaagctttcc gcacctgttc taattacatc aaaagagaag
    21661 tgaggattct ctgaatcagc cgggggaggt cacccggtct tcaggaccag gtcccccacc
    21721 ttcacgtcct gatccacctt gaggccgacc tcctgccccc ggatggcagc ggccagggtc
    21781 ttgtgctcga tctgcatcga ggtgaccatc tgcctgaaat tcgtgtgagc cccgctgatg
    21841 cgtatctcct cgccctgctt caggtcatcc gagagggcca ccaccgccac ccggatcttc
    21901 ggaaagaagt gcgtgacctt gccgatcggt ttctccatct cgtatccctc agagaccaga
    21961 taccggcccc gatacataaa ggttgtcacc tgcagccggc cgactcccgg gcttaacctc
    22021 gtaccgacgg gccctctccc tggttccgag gagcccctac ccccctggac gggaggagac
    22081 cgcttcgcgc cccttccccc cgggacagcc cggctccctc gcgggagccg gtttttcatg
    22141 cctcccgagg ggcccctttt ctggtccaga ggggcccctg cccccctgga caggaggggc
    22201 cgcttcgcgc cccctcctcc ccccgagacc gcccggctcc ctcgcgggag ccggtcttcc
    22261 ccaatcggta tcttcttttg gatggaatct gaaatcaatg gtgctggcaa gcggacggag
    22321 ggtgaaaagg cgtgtttccg gtgaggagga tgcggaggta ccggcggagg atcgtgcagg
    22381 agctcttcac cgagacctgt gtccacgtga gcgacctggt cgttcccctc ttcgtcgatg
    22441 aggcgataca ccatccgatc cctatcgaat ccatgcccgg gcaggcgagg cagccggtgg
    22501 acgacctccc ctcggtggcg gggaggatcc gggacctcga tctcccctcc gtgctcctct
    22561 tcgggatccc gaaggagaag gacccggggg cgagctccgc atatgccccc gacggggtga
    22621 tccagcggtc ggtccggtcg atgaagacgg cctctcccga cctcgtcgtc tgcacggatg
    22681 tctgtgcctg cgagtatacg gatcacgggc actgcgggat cgtgggagag aaggcaggag
    22741 agcaggacct cctgaacgat ccctcccttg aactcatgaa caaaatcgcc ctctcccatg
    22801 ccgaggcggg ggcggacatc gtggccccct cctgcatgct cgacggcatg gtcggctcca
    22861 tccggaacgc cctcgacgat gcgggcttcg aggacgtcct catcatgtcc tactcgagca
    22921 agttcgcctc cggtttttat ggcccgttcc gggaggcggc cgactccgga tacagtttcg
    22981 gcgaccggac aacctaccag ctcaacccgt ccaatgcccg ggaggcaatc ctggaatcgg
    23041 ccctcgatgc cgacgagggt gccgacatcc tcatggtgaa gccggcgggt tactacctgg
    23101 acatcctttc ctctctcagg gagtatggtc tccccctcgc cgccttccag gtgagcggtg
    23161 agtatgccat gatccgggcc gcagctgaaa aaggatggat cgatggaaga tctgccgtca
    23221 tggagagcct gacctgcatc aagcgggcgg gtgcggacct gatcatcacg tactttgccg
    23281 aggaggccgc agggtgggcg aatgaagagc cgtgagctct acgcccgggc cgttcagctc
    23341 atgcccggcg gggtgagcag ccccgtccgt gccatcaggc cctatccctt ctacaccgtc
    23401 gccgcccggg gggcccggat tatgacggca gaccgggagg atctcatcga ctgctgcatg
    23461 gcctacggac ccctcctcct cgggcattcc cacccggata tcctcgccgc catcaggaac
    23521 cagctggaga agggctggct gtacggaacc ccgacccccc tcgagcccga gatggcgggg
    23581 ctcgtccggg aggattaccc ctccatggag atgatgcgct ttgtctcgag cgggagcgag
    23641 gcgaccatgt ccgccctcag ggcggcgagg ggattcacgg ggaaaagcga catcgtgaag
    23701 gtcgaggggg ggttccacgg ggctcatgac gcggtcctcg tgaaggccgg gtcaggggcg
    23761 acgaccctcg gggtgccgga ttccgcgggg gtgcttccgg acctcacccg gcacaccctc
    23821 caggtcccgt acaatgatcc cgaggcactg gaggggctgc tcgcacggaa cagggatgtg
    23881 gcggccttca tcctggagcc ggttctcggg aacatcgggc ccatcctccc cgagaaggat
    23941 tacctgtcag aggtgcgttc catcacgagg gcccacgacg tcctcctcat cttcgacgag
    24001 gtaatcaccg gctaccgggt cggccccggc ggggcccagg ggctgtacgg catccacccc
    24061 gatctcacga ccctgggaaa gatcctcggc ggggggctcc ccatcggcgc cttcgggggg
    24121 cggagggaga tcatggatta catc
//
LOCUS       DATGDI010000215         3910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_53022, whole genome shotgun sequence.
ACCESSION   DATGDI010000215 DATGDI010000000
VERSION     DATGDI010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53022"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..551)
                     /locus_tag="VKO45_08085"
     CDS             complement(3..551)
                     /locus_tag="VKO45_08085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HMA05869.1"
                     /translation="MNPKVVIRCETDADAGAITDVTIAAFRTLEIGPHTEQFIVTALR
                     AAKALTVSLVAEVDGRVIGHIAFSQVTISDGTRNWYGLGPVSVLPKYQRQGIGKALVR
                     EGLSLLKELKAQGCCLVGHPEYYRKFGFTNEPGLLVEGVPPEVFFALSFDGHHPRGTV
                     TFHEAFKAEGQPEGEGDAIPRT"
     gene            complement(567..761)
                     /locus_tag="VKO45_08090"
     CDS             complement(567..761)
                     /locus_tag="VKO45_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05870.1"
                     /translation="MRRTCEVCGKEAIGMQIMGCCTTVVCEEHAEPMLRELKPGEIKE
                     WGICCYQRFREASPDDRDRD"
     gene            complement(782..1444)
                     /locus_tag="VKO45_08095"
     CDS             complement(782..1444)
                     /locus_tag="VKO45_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 domain-containing protein"
                     /protein_id="HMA05871.1"
                     /translation="MADQNRDNVNFFGGIPDMCRRMCRARTRGDLGALIAPLVLSYSP
                     RELQQMILNFSGQISDVEPGYRSRVTEKVSEQLLGTYQRIRLMAQQGTFLHLQDPIDA
                     SAFRFWAMVAGECRGPDGGELHLRFLKYLLAGFTMFVLREPAHAVGTPFPGGDTVQFH
                     DGVYYCPVREKANDVGGALCPFCPAVQTPAIGYLRPPVDASEHRKQEFLRTTYDHHHF
                     NG"
     gene            1731..2672
                     /locus_tag="VKO45_08100"
     CDS             1731..2672
                     /locus_tag="VKO45_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HMA05872.1"
                     /translation="MTPDIVSTSEGNIRVAFFLNLVFTVAEFAGGILFSSLAISADAL
                     HDLGDSFSLGLAWFFERLAGRKPSPFFSYGYRRFSLVAAFLNGVVLLAGGAVILWSAV
                     PRLLSPVRPDAPGMFVFAIVGIVVNGAAALRLRKGHTLNEQTVAWHLVEDLLGWAAIL
                     AVSLALMIWDVPVLDPAVSILITLVVLVNVVRNLRRTVVIFLQGVPPSLSLEAVGDRL
                     GRLPGVAGVHDLHLWSLDGEHHILTAHVIIPEETTGEESMAIKCRVKDAAMALGIQHA
                     TIEVEHPGESCRMREVCTVEPCPPSGGNRDGGSMEKD"
     gene            complement(2669..3439)
                     /locus_tag="VKO45_08105"
     CDS             complement(2669..3439)
                     /locus_tag="VKO45_08105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HMA05873.1"
                     /translation="MKPVLPLSGFNAGRVPAVLGKGSVGKQETGVSVQPPPGSLVNIS
                     VGERNYPAYFAAPDSPGKHPALVLLHSFKGLEQGYRELADRIAGDGFAVVAPGWQAQG
                     KQPPDPDVAGVIRGSLTYLSRRPEADMNRVGLTGFCAGGRYTMLFLPQMKEFRSGVAW
                     YGFPDRGTSPDGIPASHIARLSVPMLMIHGSRDEASPVKDIYRYATNLDAAGKYFELK
                     VYQGKPHGFMVVGGALARDDAAEDAYREMMAFFQRTLA"
     gene            complement(3516..>3910)
                     /locus_tag="VKO45_08110"
     CDS             complement(3516..>3910)
                     /locus_tag="VKO45_08110"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05874.1"
                     /translation="LTDSTGRALYIWLKDTTLGDSACRGTCAATWPPFSTGTLVVPSA
                     LSLTDFSTFTRSDGMRQTAFMGRPLYYYSVDKDAGDLYGQGIANVWFAANVTGNPLPV
                     TPTPTATPVTTTTVPTTTMPVYGGGGGY"
BASE COUNT          664 a         1257 c         1248 g          741 t
ORIGIN      
        1 ggtcaggtgc gcgggatagc gtcgccttca ccctccggct ggccttccgc cttgaatgct
       61 tcatggaaag tgaccgttcc ccgtggatga tgtccgtcga aggacagggc gaaaaagacc
      121 tccgggggca ccccctcgac caggagtccc ggctcgttgg tgaacccgaa tttcctgtag
      181 tattccggat gccccaccag gcagcatccc tgggccttca actcttttag tagtgacagc
      241 ccttcccgca ccagtgcctt cccgataccc tgccgctggt acttcggtaa cacggaaaca
      301 ggtccgaggc cgtaccagtt ccgggtaccg tcggaaatgg tcacctggga gaaggcaatg
      361 tgccctatca cgcggccgtc cacttctgct acaagcgaga ccgtgagggc tttcgcagcg
      421 cggagtgcag tgacgatgaa ctgctccgtg tgagggccga tctccagggt cctgaacgct
      481 gcaatggtca cgtcggtgat ggcgccggca tccgcgtcag tctcgcaccg gataacgacc
      541 ttcgggttca tgggttttct cctatttcag tcacgatcgc ggtcgtcggg ggaggcctcc
      601 ctgaaccgct ggtaacagca gatcccccat tcctttatct cgcccggctt cagttccctg
      661 agcatgggct ctgcgtgctc ctcgcacacg acggtcgtgc agcagcccat gatctgcatc
      721 ccgatggcct ccttcccgca gacttcacag gtacggcgca tgttcgatcc cctggcttcc
      781 ctcacccgtt gaagtggtgg tgatcgtagg tcgtcctcag gaactcctgc ttccggtgct
      841 cgctcgcgtc aaccgggggt ctgaggtacc cgatggccgg ggtctggacc gccgggcaga
      901 aagggcagag ggcgccgccg acgtcatttg ccttctcccg gaccgggcaa tagtacaccc
      961 cgtcatgaaa ctgcacggta tcgccgccgg ggaacggcgt gcccacggca tgggcgggct
     1021 cccggaggac gaacatcgtg aagccggcca ggaggtactt caggaaccgg agatgcagct
     1081 cccccccatc gggtccccgg cattccccgg cgaccatcgc ccagaaccgg aaggcggatg
     1141 catcgatggg gtcctgcagg tgaagaaagg tcccctgctg tgccatgagc cgaatcctct
     1201 ggtaggtgcc gaggagttgt tccgagacct tctctgtgac acgcgaacgg tagccgggct
     1261 cgacatcgct gatctggcct gaaaagttca ggatcatctg ctgaagctcc cggggtgaat
     1321 aggaaaggac aaggggggca atcagagccc ccagatcccc ccgcgtgcgt gcacggcaca
     1381 tccggcggca catatccggg atcccgccga agaaatttac gttgtccctg ttctgatcgg
     1441 ccatggttca cgggtggagt cttgaaggat gcagagttga atgttgaacc ctccgtatat
     1501 tgaatccatc gtggtggcgg ggcagccgaa gcggaaaaaa tccggaacct ggagaagagg
     1561 gggccatgga acggacgggg cattcccggt tgtccctgcc cggcccctgg tctcgtggtc
     1621 gggaatgcaa ggacctgtcc gcgcctccgt ccctccacgc atcagcggtt tctttatccc
     1681 ttcagaggaa tgaccggcag aaaggacacc cgggacagat caggtgaaag atgactccgg
     1741 acatcgtgag cacgagcgag gggaatatcc gggtcgcgtt tttcctgaac ctcgtcttca
     1801 ccgtggccga gttcgccggg gggatcctct tttccagcct ggccatatcc gccgatgccc
     1861 tccatgacct cggggactcc ttctccctcg gcctcgcctg gttcttcgag cgcctcgccg
     1921 ggaggaagcc ctcccccttc ttctcctacg ggtaccggcg cttctccctc gttgcggcct
     1981 tcctgaacgg ggtggtcctc ctcgccgggg gggcggtcat cctctggtca gcggtgcccc
     2041 ggctcctctc gccggtgagg cccgacgccc cgggcatgtt cgttttcgcc atcgtgggta
     2101 tcgtggtgaa cggtgccgcg gccctcaggc tgcggaaggg ccacaccctg aacgagcaga
     2161 ccgtggcctg gcatctcgtc gaggaccttc tggggtgggc ggccatcctc gccgtctccc
     2221 tcgccctcat gatctgggac gtcccggtcc tcgatcccgc cgtctccatt ctcatcaccc
     2281 tggtcgtcct cgtgaacgtt gtccggaacc tccggcggac cgtggtcatc ttcctccagg
     2341 gggtcccgcc ctccctctcc ctcgaggcgg tcggggatcg gctcggccgc ctccccgggg
     2401 tcgcgggtgt gcatgatctc catctctggt ccctcgatgg ggagcaccac atcctgacag
     2461 cccatgtcat catcccggag gagaccaccg gggaggaatc catggccatc aagtgccggg
     2521 tgaaggatgc cgcgatggct ctcgggatcc agcatgccac catcgaggtc gaacatccgg
     2581 gggagtcctg ccgaatgagg gaggtttgca ccgtcgagcc ctgcccgcct tcaggtggta
     2641 atcgcgatgg gggatcgatg gaaaaggatt aagcgagggt cctctggaag aaggccatca
     2701 tctccctgta ggcatcctcc gcggcatcat cccgggcaag ggccccgccc acgaccatga
     2761 aaccgtgggg ctttccctgg tagaccttca gctcgaaata cttcccggcc gcgtcgaggt
     2821 tggtcgcgta ccggtagatg tccttcaccg ggctcgcctc gtcccgggac ccgtggatca
     2881 tgagcatcgg caccgagagc cgggcgatgt ggctcgcggg gatgccatcg ggggaggttc
     2941 cccggtccgg gaacccgtac catgcgacac cggaccggaa ctccttcatc tggggaagga
     3001 agagcatcgt gtaccgcccg ccggcacaga agccggtcag gccgacccgg ttcatgtctg
     3061 cctcgggacg tcgggaaagg tacgtgaggg atccccggat caccccggcc acgtcggggt
     3121 cgggcggctg cttcccctgg gcctgccacc cgggggcgac gaccgcgaat ccgtcccccg
     3181 caatccggtc cgccaattcc cggtacccct gttcgagccc cttgaaggag tggagaagga
     3241 caagggcggg gtgtttgccg ggggagtccg gagccgcaaa atacgccgga tagttccgtt
     3301 caccgacgga gatgttcacc agggacccgg gtgggggttg caccgagacc ccggtttcct
     3361 gcttccccac ggaacccttc ccgaggacgg cagggacacg gcccgcgttg aatccagaga
     3421 ggggaaggac cggtttcatg gcaaataact caaggtccgc ctttgttgaa ccccggaggg
     3481 tttccgggtg gccccttaga gaaggaaggg aaggatcagt atcctccccc tccgccgtag
     3541 accggcattg tcgtggtcgg gaccgtggtg gtcgtgaccg gtgtcgcggt cggggtgggg
     3601 gtcaccggga gcgggttccc cgtgacgttc gcggcaaacc acacgttcgc gataccctgg
     3661 ccgtagaggt ccccggcatc cttgtctacc gagtagtagt agaggggccg gcccatgaac
     3721 gctgtctgcc gcatcccgtc cgaccgggtg aaggtgctga agtctgtgag gctcagggcc
     3781 gagggaacca ccagggtgcc ggtcgagaag ggcggccagg tggcggcgca ggtccccctg
     3841 catgcactgt cccccagcgt cgtatccttc agccagatgt agagagcccg gccggtgctg
     3901 tcggtcagga
//
LOCUS       DATGDI010000216         7838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_111235, whole genome shotgun sequence.
ACCESSION   DATGDI010000216 DATGDI010000000
VERSION     DATGDI010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111235"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..394)
                     /locus_tag="VKO45_08115"
     CDS             complement(110..394)
                     /locus_tag="VKO45_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05875.1"
                     /translation="MVTPEPSGGEDADLTEIREVKIRYEERLLSMAGVVSVGIGKGPD
                     GRPAIVVGLDRPRPEILPEIPGELEGYRVRVEIVGRARALGGPSRRNIKP"
     gene            complement(419..1423)
                     /locus_tag="VKO45_08120"
     CDS             complement(419..1423)
                     /locus_tag="VKO45_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05876.1"
                     /translation="MDENILPLRQVLRETRLDLLERENVVATGIGYKVKSGQRTGALS
                     IICSVVEKLPESRLAGRAVIPREVGGIPTDVVETGVLRALQARTDRWRPAPGGVSIGH
                     RDITAGTLGCLVRDGEGTYILSNNHVLANSNAGVPGDAILQPGPYDGGTPANDTIAEL
                     TRFVPIIFLEPAPPSDCTIGNGLAGLANLLARATGSRTRLRAVRIEAGENLADAAIAR
                     PRNAGDIRPDILDIGVLSGVRQAELGLAIRKSGRTTGMTTGEIQQVDVTVDVDYGGGR
                     TARFVDQLVAGAMSQGGDSGSAVVDAGTNLVGLLFAGSQTTTIINRIENVFAALQVHL
                     "
     gene            1647..2366
                     /locus_tag="VKO45_08125"
     CDS             1647..2366
                     /locus_tag="VKO45_08125"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HMA05877.1"
                     /translation="MVRLQDSGFFAVSEKGGREVNEDSISARSVGDFFVFGIADGLGG
                     HARGEVASEFAIAELERIDAPTLSSSDPELLILQIFAEANMRIVSYNREQGVNSGTTL
                     TACIASQDGRCTIGNLGDSRAYVIHGNTCWHTKDHSYLQQLIDSGKLSERAAIRHPQA
                     NIITRALGLERGCVPDISDMNAGGGVLVLTSDGIHDFIEPDRFREIALSPGNREAALA
                     LIDAAISGGSTDNLSVIVCRL"
     gene            complement(2377..2874)
                     /locus_tag="VKO45_08130"
     CDS             complement(2377..2874)
                     /locus_tag="VKO45_08130"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMA05878.1"
                     /translation="MRPSTNDSEPAVAHARELISVLVSHPDPEARVRAASGLGGIGGS
                     VALHALAGALHDPEKNVRREAALALATIGEPAIDTLVRALGDGDWVTRYRAAEALGSI
                     RDGRSVSALLRALGDERDHVRYMAAKGLGRLGDRQAMGRLSAALADENEFVRRAAREA
                     LAGLT"
     gene            complement(2893..3093)
                     /locus_tag="VKO45_08135"
     CDS             complement(2893..3093)
                     /locus_tag="VKO45_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05879.1"
                     /translation="MDEPDACPVMARYAIKSGVPLIGLGILLILSGFIGLIVPGIPVP
                     PFPVPEIFAGFGLFLIWAGLAK"
     gene            complement(3159..3401)
                     /locus_tag="VKO45_08140"
     CDS             complement(3159..3401)
                     /locus_tag="VKO45_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05880.1"
                     /translation="MNARIKVPPDVFEVLETLRAGGFLDIARKEKEKGFLIALTHCKF
                     EYEKKKDAKFMNTYEWLKRHKHEFFWGVLDGFEIGG"
     gene            complement(3488..3745)
                     /locus_tag="VKO45_08145"
     CDS             complement(3488..3745)
                     /locus_tag="VKO45_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05881.1"
                     /translation="MAQGERNPSIRVPHRVYELLGDPGLAEFMEMTRENHERGFVMLE
                     SHLLHESDRTGDRRYGDLLEWLRKHRQEFFFGVSRGFEDAR"
     gene            complement(3807..5234)
                     /locus_tag="VKO45_08150"
     CDS             complement(3807..5234)
                     /locus_tag="VKO45_08150"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HMA05882.1"
                     /translation="MDLTANEKRLLASLGKRRRADPAGLASDLGTTEEAVLQYARLLA
                     DRALVAIQKEVRKSYTLTDEGKEYREKGLPERQLLESFGESIPIKDLQRNPHAKLGIG
                     WMRKKGWVTIRDGVVTKAGTPPPGPDENALRSPGSGGDGIRALVDRGLLTEREEAAYE
                     ILLTPAGEEALSAGLGTGEEVGTLTREQILSGSWKGLPLRKYRLETHPKPVIPGKVHP
                     YQRLMSEMREILLDMGFTELSGGLVQSAFWNFDALFQPQDHPAREMQDTFYLDDCEPL
                     PAGYERVKAAHENGAGTGSTGWGGTWSGAKAEQCVLRTHTTLLSIQHLAANPKPPVKA
                     FCIGRVYRREAIDPTHLPEFEQLEGIVMDEGVNFTHLLGYLTEFYHRMGFSGVRFRPG
                     YFPYTEPSVEPEVFVEGLGWVELGGAGIFREEVTRPWGIEHPVLAWGLGISRVAMLTL
                     GLKDLRQLYRSDIAWIRESPVRRKA"
     gene            complement(5238..6512)
                     /locus_tag="VKO45_08155"
     CDS             complement(5238..6512)
                     /locus_tag="VKO45_08155"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HMA05883.1"
                     /translation="MKGEGAGEAGKAVNPWGNSQAQDYQAIFSEFGIEPISTVIPELP
                     ELPPFIRRGIVVGHRDYQAIARAIREGTPYYVMTGFMPSGHPHLGHLMVMKEVVWHVR
                     QGATGYIAIADREAHAVRGISWEKCREYGREYLQLLRALGYEGTSYYQSENHPLHGLA
                     FEASTKVNFSELSAIYGFGPDTSLSHAESVITQVADILFPQVDRGPAPTVVPVGVDQD
                     PHLRLTRGIAHKLRMFTVEDRDGYVSVRGKNAPPEAVEAVHAAFPGSKKFEGHVDIRG
                     CPLRDVEERAREIEREHGGYGFFIPSSTYHIFMPGLQGGKMSSSVPESLFSFGEKDED
                     IARKVKNALTGGRMTVEEQKRLGGEPDKCSIFLLDLFHLVEDDRELEEIRRACREGRL
                     LCGPCKKAALDRVWAFLRDFRERMDAAAPGGG"
     gene            complement(6509..7516)
                     /gene="endA"
                     /locus_tag="VKO45_08160"
     CDS             complement(6509..7516)
                     /gene="endA"
                     /locus_tag="VKO45_08160"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HMA05884.1"
                     /translation="MKGRLDGSSVLLGPDGMVLHEQGGFGRVERAGLRLSPEEALYLV
                     HREKVEVEGYPFEALLGAFADRPGLFRSFLVYRDIRERGYAVQPGLDHYRLFRRGERP
                     GTGRSRLLVRVLSERDLVDFEVIRSEVETSGHMRKDHVLAVVDDEGELTYYGVKVTDL
                     ARVSEPPVSEPCEGRLFGTTVVLPGPIPAGVRDAWFGTPLDEGRLILSPVEALYLMRR
                     GALVLPGEAGPLSPEAYLERTLPSDPELAEKAAVYADLRDHGYIPRTGYKFGHHFRVY
                     AGDRSHSDLLVQASPPAQMPAVSVISRSVRLAHSVKKKMLFACVHHSGIRYIEFGRMK
                     L"
     gene            complement(7581..>7838)
                     /locus_tag="VKO45_08165"
     CDS             complement(7581..>7838)
                     /locus_tag="VKO45_08165"
                     /inference="COORDINATES: protein motif:HMM:NF039186.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HMA05885.1"
                     /translation="SSAVIFLGLYSAYFLTLEVAAPGYVQRVWDLRVLSGVSLLGIPL
                     EELGFGFAFGFFWSGVYEYALGFRVRRKEEKASSRPPAYGE"
BASE COUNT         1301 a         2689 c         2471 g         1377 t
ORIGIN      
        1 gctcctctcg agccgctccc cgggggtcag ggcctcccgc cgctcgcgca tggcctggcg
       61 gacccggttc ttccgatccc tcatagggag aagtgggtcc cgtgggtatt tatggcttga
      121 tattccggcg tgaagggccc ccgagggccc tcgccctccc gacgatctct accctcaccc
      181 ggtagccctc gagctccccg gggatctccg gaaggatctc cggccgcggc cggtccaggc
      241 cgacgacgat ggccggccta ccgtcggggc ctttaccgat tcccaccgag acgactccag
      301 ccatcgagag gaggcgctcc tcgtagcgga tcttcacctc ccggatttca gtcaggtccg
      361 catcctctcc accggacggt tcgggtgtca ccatggaacc ccctcagctc cgattccttc
      421 agagatgcac ctgcagggct gcgaagacat tctcgatccg gttgatgatg gtggtcgtct
      481 ggctcccggc gaagaggaga ccgaccaggt tcgtcccggc atccaccacc gcagacccgc
      541 tgtcccctcc ctggctcatc gcgcccgcaa cgagctgatc gacaaacctg gccgtgcggc
      601 ccccgccgta atcgacatcg accgtcacgt ccacctgctg gatctcgccc gtggtcatgc
      661 cggtggtccg gccgcttttc ctgatggcaa gcccgagctc ggcctgcctc acgcccgaaa
      721 ggaccccgat gtccaggata tcgggcctga tgtccccggc attccggggg cgcgcgatgg
      781 cggcatctgc caggttttcc ccggcctcta tcctcacggc ccggagacgg gtacggctcc
      841 ccgtcgcccg ggcgaggagg tttgcgagcc ccgcgagccc gttcccgatg gtgcagtcac
      901 tcggaggcgc cggctccagg aatatgatcg ggacgaagcg cgtcagttcg gcgatggtat
      961 cgtttgccgg agtgcctccg tcatagggcc cgggctgcag gatggcatcg cccgggacac
     1021 cggcgttact gttcgcgagg acatggttgt tggagaggat gtaggtcccc tccccgtccc
     1081 ttaccaggca tcccagggtc cccgccgtga tatcccggtg gccgatgctc accccgccgg
     1141 gcgcgggacg ccaccggtcc gtccgggcct gcagggcccg gagcaccccc gtctccacca
     1201 cgtcggtcgg gatccccccc acctcccggg ggataaccgc cctgcccgcg agccgggact
     1261 cgggcagctt ctccaccacc gagcagatga tggagagggc cccggtcctc tgcccgctct
     1321 tcaccttgta gccgatgccc gttgccacca cattctccct ctccagcaga tcgagccggg
     1381 tctcccggag gacctggcga agaggcagga tattctcgtc catcgttttc ctcccccccc
     1441 gtgattcaac gatcccggcc ggctccatta cctggatgag agaagtccgg gatcttcaca
     1501 cgagacaggg aaaccagggg atttataggt atgggacggg tcccggaaga gtcccgtgag
     1561 aaccggtcgg cccgggatgc cggaccggac aagccttttt accgcttgcg ggcaaccatc
     1621 aggagtgaag ggtcaggcct tcccgggtgg tccgtctgca ggattccggc ttctttgccg
     1681 tctcggagaa gggcgggagg gaggtgaacg aggactccat ctccgcccgg tctgtcgggg
     1741 actttttcgt gttcgggatt gcggacggcc tcggggggca tgcccggggg gaggtggcga
     1801 gcgagttcgc cattgcggag cttgaaagga tcgatgcccc cactctctcc tcctccgatc
     1861 ccgaacttct gatcctccag atttttgccg aggcgaacat gcggatcgtg agctacaacc
     1921 gggaacaggg ggtcaactcc gggacgaccc tcaccgcatg catcgcctcc caggacgggc
     1981 ggtgcaccat cgggaacctc ggcgacagcc gggcctatgt catccacggg aacacctgct
     2041 ggcacacgaa ggatcacagc tacctccagc agctcatcga ttcggggaaa ctctcggaac
     2101 gggcggcgat ccgccatccc caggccaaca tcatcacccg ggccctcggc ctggagaggg
     2161 gatgcgtacc tgatatctcg gacatgaacg cgggaggagg agtgcttgtc ctcacgtccg
     2221 acgggatcca tgacttcatc gagccggacc ggttccggga gatcgccctc tccccgggaa
     2281 accgcgaggc ggcccttgcc ctcatcgatg ccgcgatctc cggcgggagc accgacaacc
     2341 tctcggtgat cgtctgccgg ttgtaggagg cggattttac gtgagccccg cgagggcctc
     2401 cctcgcagcg cggcggacga actcgttctc gtctgcgagg gccgcggaga gccggcccat
     2461 cgcctgcctg tcccccagcc tcccgaggcc cttcgccgcc atgtaccgga catggtcccg
     2521 ctcgtcaccg agagcccgga ggagagcaga gaccgagcgg ccgtccctga tggaccccag
     2581 ggcctctgcc gcacggtacc gggtaaccca gtccccgtcc ccgagtgccc tgacgagggt
     2641 atcgatggcc ggttcgccga tggtagcgag ggccagggcc gcctcccgcc ggacgttctt
     2701 ttccggatcg tgaagggccc cggcgagggc gtgtaacgcc acactgccgc cgatcccgcc
     2761 aagccccgaa gcggcccgca cccgtgcctc gggatccggg tgcgagacga ggacggagat
     2821 taattctctc gcatgagcca ccgcaggttc actgtcgttc gtggaaggac gcatgatctg
     2881 ctccccggtt cgctatttcg caagcccggc ccagatgagg aagaggccga agccggcgaa
     2941 gatttccggg acggggaagg ggggtacggg gatacccggg acgatgagcc cgataaagcc
     3001 tgaaaggatc aggaggatac cgagcccgat gaggggaacc cccgatttga tggcataacg
     3061 ggccatgacc gggcaggcat caggttcgtc catgtctcat ccccctcccc gcggaaaaac
     3121 gcacgggcat tcgtccattc tcttcaggtt ccccggtatt acccgccgat ctcgaacccg
     3181 tcgagaaccc cccagaagaa ctcgtgcttg tgcctcttca gccactcgta ggtattcatg
     3241 aacttcgcat ccttcttctt ctcatactcg aacttgcagt gggtgagggc gatcaggaac
     3301 cccttctcct tctctttcct tgcgatatcg aggaaccctc cggctctcag ggtctcgagg
     3361 acctcaaaaa catccggggg aaccttgatc ctggcattca tggggggtcg gccggtcttt
     3421 gagctagcag tacatcgaat ggatatatat tagaattgtt tcttctcttc cgagccttcg
     3481 ggtaccttca ccgggcatcc tcgaacccgc gggagacccc gaagaagaac tcctgcctgt
     3541 gcttcctcaa ccactccagg aggtccccgt accgccggtc ccccgtccgg tccgactcgt
     3601 ggagaaggtg actctcgagc atcacgaagc cccgctcatg gttctccctc gtcatctcca
     3661 tgaactcggc gagcccggga tcccccagga gctcgtagac ccggtggggc acccggatcg
     3721 acggattcct ttccccctgc gccatgggag atactcgggg agcatggtat aaaaagcggc
     3781 ctgtcgccgg ggagaaggcc ttcgggctac gccttccttc gcaccgggct ctcccggatc
     3841 caggcgatat cggaccggta gagctgccgg agatccttta acccgagggt gagcatggca
     3901 accctcgaga tgccgagacc ccaggcaagg accgggtgct cgatccccca cggccgggtg
     3961 acctcctcgc ggaagatccc ggccccgccg agctccaccc agccgaggcc ctcgacgaag
     4021 acctccggct ccaccgaggg ctcggtgtac gggaaatacc cgggccggaa ccggaccccg
     4081 gaaaagccca tccggtggta aaactcggtc aggtacccga gaaggtgggt gaagttcacc
     4141 ccctcgtcca tcacgatccc ctcgagctgc tcgaactccg ggaggtgggt gggatcgatg
     4201 gcctcgcgcc ggtacaccct gccgatgcag aaggccttca cgggcggctt cgggttcgcg
     4261 gcgaggtgct ggatggagag gagggtggtg tgagtcctga gcacgcactg ctccgccttc
     4321 gccccggacc aggtgccacc ccagccggtc gatccggtcc cggccccgtt ctcatgggcc
     4381 gccttcaccc gctcatatcc cgccgggagg ggctcgcaat catcgagata gaaggtgtcc
     4441 tgcatctccc gggccgggtg atcctggggc tggaagaggg catcgaagtt ccagaaggcc
     4501 gactggacga gtccccccga gagctcggtg aaccccatgt cgaggaggat ctcccgcatc
     4561 tcgctcatga gccgctggta gggatgcacc ttgcccggga tcacgggctt cggatgggtc
     4621 tccagccggt actttctgag ggggagtccc ttccagctcc cggaaaggat ttgctcccgg
     4681 gtgagggtcc ccacctcctc gcctgtaccg aggccggctg aaagggcctc ctccccggcg
     4741 ggggtgagga gaatttcgta cgcggcctcc tccctctcgg tgagaagccc gcgatccacg
     4801 agggcacgga tcccgtcacc gccggatccg ggcgagcgga gggcgttctc atcgggtccg
     4861 gggggcgggg tcccggcctt tgtcacgacc ccgtccctga tggtcaccca tccttttttc
     4921 ctcatccacc cgatcccgag tttcgcatgg ggattcctct ggagatcctt tatcgggatg
     4981 ctctccccga agctctccag gagctggcgt tccgggagac ccttctcccg gtactccttc
     5041 ccctcatcgg tgagggtgta gctcttcctt acctccttct ggatcgctac gagggcccgg
     5101 tcagcaagca ggcgggcgta ctggaggacc gcctcctccg tcgtccccag gtccgatgca
     5161 agcccggcgg ggtcagcccg cctcctcttc ccgagggagg cgaggagccg tttctcgttc
     5221 gcggtcaggt ccacggttta cccacctccc ggcgcggcgg catccatcct ctcccggaaa
     5281 tcccggagga aggcccagac ccggtccagg gccgccttct tgcagggacc gcagaggagg
     5341 cgtccctccc ggcaggccct ccggatctcc tcgagctccc ggtcgtcctc caccaggtgg
     5401 aagaggtcga ggaggaagat ggagcactta tccggttccc cgccaagccg tttctgctcc
     5461 tccacggtca ttctcccccc ggtgagggcg ttcttcacct tcctcgcgat gtcctcgtct
     5521 ttttccccga aggaaaagag gctctcggga accgaggagg acatcttccc cccctggagg
     5581 ccgggcatga agatgtggta ggtcgagctg gggatgaaga acccgtaccc cccgtgctcc
     5641 ctctcgatct ccctcgcccg ctcctccacg tcgcggaggg ggcagccccg gatgtccacg
     5701 tgtccctcga actttttcga accggggaag gccgcatgga cggcctccac cgcctccggc
     5761 ggggcgttct tccccctgac cgagacgtac ccgtcccggt cctcgacggt gaacatccgg
     5821 agtttgtggg cgatcccccg ggtcaggcgg agatgggggt cctggtcgac ccccacgggg
     5881 accacggtcg gggccgggcc ccggtccacc tgggggaaga ggatgtcggc cacctgggtg
     5941 atgacgctct ccgcgtggga gagggaggtg tccgggccga agccgtagat ggccgagagc
     6001 tccgagaagt tcaccttcgt ggatgcctcg aaggcgaggc cgtggagggg gtggttctcg
     6061 ctctggtagt agctcgtccc ctcgtagccg agcgcccgga ggagctggag gtactcccgg
     6121 ccgtactccc ggcacttttc ccaggagatg ccccggacgg cgtgggcctc ccggtcggca
     6181 atggcaatgt agcccgtcgc cccctgccgg acgtgccaga cgacctcctt catcaccatg
     6241 aggtgcccga ggtggggatg tcccgagggc atgaacccgg tcatcacgta atagggggtt
     6301 ccctcgcgga tcgcccgtgc gatggcctgg taatcacggt ggccgaccac gatcccccgc
     6361 cggataaagg gggggagctc gggcagctcg ggtatcacgg tggagatggg ctcgatgccg
     6421 aactccgaga agatggcctg gtagtcctgg gcctggctgt tcccccaggg attgaccgcc
     6481 ttccccgcct ccccggcccc ctctcccttc acagtttcat cctcccgaac tcgatgtacc
     6541 ggatgcctga gtggtgtacg caggcaaata acatcttctt tttcacggaa tgggcgagcc
     6601 ggaccgagcg ggagatcacg gagacagcgg gcatctgtgc gggtgggctt gcctggacga
     6661 ggaggtctga gtggctccgg tccccggcat acacccggaa gtggtggccg aacttgtaac
     6721 ccgtccgcgg gatgtagcca tggtccctca ggtccgcgta caccgcggcc ttttctgcga
     6781 gctcggggtc cgagggcagg gtacgttcca ggtacgcctc cggggaaagg ggcccggcct
     6841 cacccgggag gacgagggcg ccccggcgca tcaggtagag ggcctcgacc ggcgagagga
     6901 tgagccttcc ctcgtcgagg ggcgtcccga accacgcgtc cctcaccccg gcagggatcg
     6961 ggccggggag aacgaccgtg gtcccgaaga gccggccctc gcagggctcg ctgaccggcg
     7021 gttccgagac cctggccagg tcggtcacct tcaccccgta gtacgtaagc tccccctcgt
     7081 catctactac ggcgaggaca tggtccttgc gcatgtggcc ggaggtctcc acctcgctcc
     7141 ggatcacctc gaaatcgacg agatcccgct cggagaggac acggacgagg agcctcgacc
     7201 tgcccgtccc cggccgctcg cccctccgga agaggcggta atggtcaagc cccggctgca
     7261 ccgcgtatcc gcgttccctg atgtcccggt acacgaggaa gctccggaag aggccgggcc
     7321 ggtccgcaaa ggccccgagg agagcctcga aggggtatcc ctccacctct accttctcgc
     7381 ggtggacaag gtacagggcc tcctccgggg agagcctgag gccggcccgc tccaccctgc
     7441 cgaaaccgcc ttgctcgtgg agaaccatcc cgtccggccc caggagaacc gaggatccgt
     7501 caagcctccc cttcacggga gataatgggg ggcgtcctct ctttataccg atggatcgcc
     7561 cccgggtctc ccgtcgccgt ctactccccg taggcagggg gccgggagga ggctttctcc
     7621 tccttcctcc tcacccggaa cccgagggcg tactcgtaca cgccggacca gaagaacccg
     7681 aaggcgaagc cgaagccgag ctcctccagg ggaatcccga gaaggctcac tcccgagagc
     7741 acccggaggt cccaaaccct ctggacgtac ccgggcgccg cgacctccag ggtgaggaag
     7801 taggcggagt agagcccgag gaagatcacc gcactcga
//
LOCUS       DATGDI010000217         1528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_402040, whole genome shotgun sequence.
ACCESSION   DATGDI010000217 DATGDI010000000
VERSION     DATGDI010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402040"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VKO45_08170"
     CDS             <1..1081
                     /locus_tag="VKO45_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05886.1"
                     /translation="ACSQSLYDAERVRPDFYEVLTPRVEVLDEYSNALRIAGLYPLVT
                     IPEYKITERVRERFRAWYEMPYEKLPVEGTLRVYRPPQGTGRDAREVIAASRRNPLGT
                     PRPSAGEAREMALHYAPVIIQDEAAPYDRVGRISWKGDELTVDGDKPAVYYYLSHARV
                     KGEPVLQINYVFWYAARAGDRAPSIEHGRLDGLTLRLSFEPGGRLFMIDLMNNCGCYH
                     AFIPAKDRVLRTIQRYYGNDPFAPQGLPAMAAGQSPAVRIISGWHQVQRLLALAPPPE
                     GTPYELLPYEMLEGLPRGDDRGASIFDAEGIAKGSGRVERFIFFPTGIPSIGSMRQRG
                     HHAITLSGRTLFDEPGLFEHYFVFR"
     gene            1083..>1528
                     /locus_tag="VKO45_08175"
     CDS             1083..>1528
                     /locus_tag="VKO45_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05887.1"
                     /translation="MKRCILMIAIALLTLHPPDCRAQGGTDRERILEVANRIEEAYGK
                     LRSYACDIEGIYFESGRELERYDFRFYFRKTDRFRIEFRSPYPGVTIFYTEGEKEFTS
                     RPFAAFPAVQFRFSVNNPLFKSPSGQAVNQLHLRYFLGFLRDNARTL"
BASE COUNT          280 a          502 c          461 g          285 t
ORIGIN      
        1 ggcctgttcg cagtctcttt acgatgccga gcgggtacgg cccgattttt acgaggtgct
       61 cacgccccgt gtcgaggtcc tcgacgagta ctccaatgcc ctgcggatcg ccgggctcta
      121 tcccctggtc acgatccccg agtacaaaat caccgagagg gtgcgcgaac gcttccgcgc
      181 ctggtacgaa atgccttacg aaaaactccc cgtggaggga acgctgcgtg tctacaggcc
      241 gccgcagggc accggcaggg atgcccgcga ggtgatcgcg gcctcgcgga gaaatcccct
      301 gggcaccccc aggccctccg ccggcgaggc aagggagatg gccttgcact acgcgcctgt
      361 catcatccag gacgaggcag ccccttatga ccgggtcggg aggatctcct ggaagggcga
      421 tgaactgacc gtcgacgggg acaagcccgc ggtctattat tacctctccc atgcccgcgt
      481 gaagggcgaa ccggtccttc agatcaacta tgtcttctgg tatgcggccc gggcgggaga
      541 ccgggcgccc tcaatcgagc acggccgtct cgacggcctc acccttcgcc tgtccttcga
      601 accaggggga cggctgttca tgatcgatct catgaacaac tgcggctgct atcacgcatt
      661 catcccggcg aaggaccggg tgctccggac gatccagcgc tattacggaa acgacccctt
      721 cgccccccag ggactcccgg cgatggcggc ggggcaatcc cccgccgtac ggatcatctc
      781 gggatggcac caggtccaga ggcttttggc cctggcaccc ccgcccgaag gcactcccta
      841 tgagctgctc ccctacgaga tgctggaggg cctgccccgg ggggacgacc ggggcgcaag
      901 catcttcgac gccgagggca ttgccaaagg ctccgggagg gtcgagcggt ttatcttttt
      961 ccccacgggc attccttcca tcgggagcat gaggcagcgg ggccaccacg ccatcaccct
     1021 gtcggggcgg accctttttg atgaaccggg tctgttcgag cattacttcg tgttcaggtg
     1081 agatgaagcg ctgcatcctg atgattgcaa tagccctgct gaccctgcat cccccggact
     1141 gccgggccca gggcggtacg gaccgggagc ggatcctcga ggtggcaaac cggatcgagg
     1201 aggcatacgg caagctccgc tcctacgcct gcgatatcga agggatctat ttcgagtcag
     1261 gaagggagct cgaacgctac gacttccggt tctatttccg caagacggac cgcttccgga
     1321 tcgagttccg aagcccttac ccgggggtga cgatcttcta caccgagggg gagaaagagt
     1381 tcacctcgag gccctttgcc gcattccctg cggtacagtt ccgtttttcg gtcaacaacc
     1441 ccctgttcaa gtccccctcg ggacaggccg taaaccagct gcacctccgt tacttcctgg
     1501 gatttctgcg cgataacgcc aggaccct
//
LOCUS       DATGDI010000218         1666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_227637, whole genome shotgun sequence.
ACCESSION   DATGDI010000218 DATGDI010000000
VERSION     DATGDI010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227637"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VKO45_08180"
     CDS             <1..1088
                     /locus_tag="VKO45_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HMA05888.1"
                     /translation="WQGWPRVKIRVPDGAQKGPGEEGSPPSRAVEAIAPLIISASRST
                     DIPAFYGDWFMDRLGKGYAKWINPWNGIPMYVSFGDARVFVFWSKNPEPFLPHLAELE
                     RLGNRYLVLFTLNDYEAERAEPNIPPLEDRIRTFQAISRMAGKGRVAWRWDPLLLSGS
                     LGVKGLLDRIGRVGDAIAPFTDRMIISFIDIARYPRVARLLRNRGFGDIREFSPAEER
                     ELATGLQELNRSWGLTISACGEKRDLSRFGIGAGNCISREVLLREFAGDPVLGRFLDV
                     PDQGTPSGSRETSPVPPHLKDPGQRRACGCVVSKDIGQYATCPHLCAYCYANTAPARV
                     MGRYEAYRADARMGVFGESITEEKKKD"
     gene            complement(1140..>1666)
                     /locus_tag="VKO45_08185"
     CDS             complement(1140..>1666)
                     /locus_tag="VKO45_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05889.1"
                     /translation="GCEPQPKTDPPELGGIPAPRVRAGRQHILETAFGLVSSTPEVRY
                     LMSFFVRMRYCPETDSCQVVIQPHYSPAGEYDGEIRNEIVAPLSRQIRASLGGKISRK
                     TCAYIASASAKDEDKLADLFILYFFAGEGPGTPGDHIWGVYQMNLAFRTFAPEMEEIA
                     VNSLERCLCVIQQG"
BASE COUNT          298 a          506 c          547 g          315 t
ORIGIN      
        1 gatggcaggg ctggccgagg gttaaaatcc gggttccgga tggtgcacag aaaggccccg
       61 gggaagaggg atctcccccc agccgcgcgg tcgaggccat cgccccgctc atcatctcgg
      121 catcgcgatc gacggacatc cccgccttct acggcgactg gttcatggat cgcctcggga
      181 agggatatgc gaagtggatc aatccctgga acggtattcc gatgtacgtc tccttcgggg
      241 atgcccgggt ctttgtcttc tggtccaaga accctgagcc tttcctcccc catctcgccg
      301 agctggagcg gctcgggaac cggtacctgg tgctcttcac cctgaacgac tatgaggccg
      361 aaagggccga gccgaatatt ccccctctgg aggaccgcat ccggaccttc caggcgatct
      421 cccggatggc gggaaagggg cgggtagcct ggagatggga tcccctcctc ctgtccgggt
      481 cccttggcgt gaaagggttg ctggaccgaa tcggccgggt gggtgatgcc atcgccccct
      541 tcacggaccg gatgatcatc tccttcatcg acatcgcgag gtacccgagg gtggcccggc
      601 tcctcaggaa ccgggggttc ggggatatca gggaattctc acccgccgag gaaagggaac
      661 tcgccacggg tctccaggag ctgaacaggt catggggcct cacgatatcc gcctgcggag
      721 agaaaaggga cctctcccgt ttcgggatcg gggccggaaa ctgcatctcc cgggaagtcc
      781 ttctccggga gttcgctgga gatccggtcc tcggccggtt cctggatgtt ccggatcagg
      841 ggactccctc cggttcccgg gagacctccc cggtgccccc gcacctgaag gatcctggcc
      901 agcgccgggc atgcggctgc gttgtctcca aggacatcgg gcagtacgcc acctgccccc
      961 acctctgtgc ctactgttat gcgaataccg ccccggcgag ggtcatggga cggtatgaag
     1021 cctaccgggc tgatgcccgg atgggtgtct ttggcgaatc catcaccgaa gagaaaaaaa
     1081 aggattgaac ccggggcagc gccgggggcg aggacgggat tctctggaaa ggatgattct
     1141 tatccctgct ggatgacgca gaggcatctc tcgagggaat tcacggcaat ctcctccatc
     1201 tccggtgcga aggtgcggaa ggccaggttc atctggtaga ccccccagat atggtctccg
     1261 ggcgtgcccg gcccctcgcc ggcaaagaag tacaggataa agagatccgc gagcttgtcc
     1321 tcgtccttcg ccgaggcgga ggcgatgtat gcgcaggttt tcctcgagat cttccccccg
     1381 agggatgccc ggatctgtcg cgagaggggg gccacgattt cgttcctgat ctccccgtcg
     1441 tattcgccgg ccgggctgta gtgcggctgg atgacgacct ggcacgaatc cgtttccggg
     1501 cagtacctca tccggacgaa gaaggacatc aggtacctga cctcgggtgt tgaggaaaca
     1561 aggccgaagg cggtctcgag gatatgctgc cggccggcac ggacccgggg ggcgggtatt
     1621 cccccgagtt caggggggtc ggttttcggc tggggttcgc aaccgg
//
LOCUS       DATGDI010000219         4033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_343750, whole genome shotgun sequence.
ACCESSION   DATGDI010000219 DATGDI010000000
VERSION     DATGDI010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343750"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VKO45_08190"
     CDS             complement(<1..551)
                     /locus_tag="VKO45_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HMA05890.1"
                     /translation="MKSDEQKRQDQGPVVPLSNGEESNRGAPPDGTVTQDPLRQKANR
                     YNRQLLLPEIGVEGQRRLGESRVTIVGMGALGSCAAGTLVRAGVGKVVLIDRDLVELH
                     NLQRQVLYTEEDLDRPKALAASEILKKVNTTVEIEAHAVDLNPSNAEELLKDSDVVLD
                     GTDNLQTRFLVNDVCVKHRIPWI"
     gene            complement(582..1541)
                     /gene="cysE"
                     /locus_tag="VKO45_08195"
     CDS             complement(582..1541)
                     /gene="cysE"
                     /locus_tag="VKO45_08195"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HMA05891.1"
                     /translation="MSVSEDIRNVFARDPAARSTIEVFFCYPGLHAVWFHRFNHWLWT
                     HRLLFAARFFSHISRFLTGIEIHPAAKIGKRVFIDHGMGVVIGETAEVGNDVLIYMGV
                     VLGGTALEKVKRHPTIEDGVVIGSGASVLGPITVGKGARIGAGAVVVRPVPPGATVVG
                     VPGRIAGPECTPKKPDPVHGDMPDPMLRVISRLLDRQNQLEERLRSLEMAQPPVSEEE
                     SGETILVETEVRRALSEVIDPEVGIDIIDLGLVKQVRVTDHRVEVDLVLTTTGCPLVN
                     YLTEQVKKRVMTVPGIEAVDVKILDEPWDWDRFVTPKGVLKEI"
     gene            complement(1555..2484)
                     /gene="cysK"
                     /locus_tag="VKO45_08200"
     CDS             complement(1555..2484)
                     /gene="cysK"
                     /locus_tag="VKO45_08200"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HMA05892.1"
                     /translation="MSQIYGDITETVGGTPLVRINRLTEGLEATVVAKLESFNPMNSV
                     KDRIGLSMIEAAEKENLIGKDTTILEPTSGNTGIALAFVCAARGYRLTLVMPETMSVE
                     RRKIVKAFGAELILTPGSEGMRGAVSRAEKMAAENPHYIYIPQQFRNPANPAIHRRTT
                     AEEIWRDTDGKVDAIVSGVGTGGTITGTAEIIKPRKPSFLAIAVEPVESPVLSGGKPG
                     PHKIQGIGAGFVPDVLRRDLLDEILQVSYPDAVATSRRLAKEEGIFAGVSAGAAMTAA
                     LRVAARPEMKGKLIVAILPDSGERYLSTELFEA"
     gene            2947..3246
                     /locus_tag="VKO45_08205"
     CDS             2947..3246
                     /locus_tag="VKO45_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05893.1"
                     /translation="MGADTGKKITSIGSIIENPTAYENRTVTISGKIVLECGSGCWFN
                     LDDGSGTIYVDLKPHNFIIPQLGGEKAVVTGTVAIRDNTPRIFGEKVEVGGKVYP"
     gene            3243..>4033
                     /locus_tag="VKO45_08210"
     CDS             3243..>4033
                     /locus_tag="VKO45_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA05894.1"
                     /translation="MNHLSLAVKNLRRRRGRTLLTITGVAIAVAVLFSLLAFDAGYEQ
                     SLNREMKGLGVHLLAVPKGCPYEAASLILHGGVIPKYLSGDDLARVRALDGVELAAPM
                     LLQQFVKNGTPHVVYGIQGSDIPGLKPGWSVKGRFFSDGESRVMLVGSDLAAEEGLEV
                     GSVLPFGKDREPFTVVGILERTGGQDDNFHFVPLAEAQRIFGLEGKITAIAVRLKDIG
                     SISDISTEMEQIPDIQVVTMAQVQGTILSLVGSARTLILSVIVVA"
BASE COUNT          693 a         1260 c         1255 g          825 t
ORIGIN      
        1 tagatccagg ggatccggtg tttcacacag acatcgttga ccaggaaccg ggtctggagg
       61 ttgtcggtcc cgtcaaggac cacgtcagaa tccttgagga gctcctcggc gttcgatggg
      121 ttcaggtcga ctgcatgagc ctctatctca actgtagtat tgaccttttt caggatctca
      181 ctggcagcga gcgccttcgg acggtcaagg tcctcctcgg tatacaggac ctgccgctgc
      241 aggttgtgaa gctccacgag atcacggtcg atgaggacga cctttcctac tcctgcccgt
      301 acgagggtgc cggcggcaca acttccgagg gctcccatcc cgacgatggt cacccggctc
      361 tccccgagtc gtcgctgccc ctccacccct atctccggga ggaggagctg gcggttgtag
      421 cgattcgcct tttgacggag aggatcctgc gtaacggtcc cgtccggtgg agccccccgg
      481 ttactctcct ctccattaga caacggtacg acggggccct ggtcctgccg tttctgttcg
      541 tcggacttca tttgggggct ctctcctggt gccggatctc ctcagatttc cttcaggact
      601 ccttttgggg tgacgaaccg gtcccagtcc cagggctcgt caaggatctt cacatccacc
      661 gcctcgatgc cggggacggt catcacccgc ttcttcacct gctcggtgag gtagttcacc
      721 agggggcagc cggtcgtggt gaggaccaga tccacctcca cgcggtggtc cgtgacccgg
      781 acctgcttga cgaggccaag gtcgatgatg tctatcccga cctccgggtc gatgacctcg
      841 gaaagggccc tcctcacctc ggtttcgacg aggatcgtct ctcctgattc ctcctcagag
      901 acggggggtt gcgccatctc caggctccgg agccgctcct cgagctggtt ctgccggtcc
      961 aggagccggg agatgaccct gagcatcggg tcgggcatgt ccccgtgcac ggggtcgggt
     1021 ttcttcgggg tgcattccgg tccggctatc cggcctggga cgcccaccac ggtcgcgccg
     1081 ggaggtacgg gccggaccac gaccgcccct gcaccgatcc tcgctccctt ccccacggtg
     1141 atgggaccga gcaccgatgc cccggagccg atgacgacac cgtcctcgat ggttgggtgg
     1201 cgtttcacct tctcgagggc tgtcccgccg aggactaccc ccatgtagat gaggacatcg
     1261 ttccccacct cagcggtctc cccgatgacc actcccatcc catggtcgat gaagaccctc
     1321 ttcccaatct tcgccgcggg atggatctcg atccccgtca ggaaccggga gatgtgggaa
     1381 aagaaccggg ccgcaaagag cagtctgtgg gtccagagcc agtggttgaa ccggtggaac
     1441 cagaccgcat ggagccccgg gtagcagaaa aatacctcga tggtcgagcg tgctgccggg
     1501 tcccgggcga agacgttcct gatatcctca ctgacactca tggttcgttc ctgctcatgc
     1561 ctcgaagagc tcggtcgaga ggtaccgctc gccgctgtcg gggaggattg ccacgataag
     1621 cttccccttc atctcagggc gtgcggcaac cctgagagca gcggtcatcg ccgcccccgc
     1681 ggacaccccg gcaaagatgc cctcctcctt tgcgagcctt ctggaggtgg cgaccgcatc
     1741 agggtacgag acctggagta tctcgtcgag gagatcccgc cggaggacgt ccgggacgaa
     1801 gccggcaccg atcccctgga tcttatgggg cccgggtttt ccgcccgaga ggaccgggga
     1861 ttcaacgggt tccaccgcga tggcgaggaa agagggcttc cggggcttga tgatctccgc
     1921 ggtcccggtg atggtgccac cggtgccgac ccctgagaca atggcatcca ccttcccgtc
     1981 ggtatcccgc cagatctcct cagcggtggt ccttctgtgg atggccgggt tggccgggtt
     2041 ccggaactgc tgggggatat agatgtaatg ggggttctct gcggccatct tctcggcgcg
     2101 ggagaccgcc ccgcgcattc cttctgagcc cggggtgagg atcagttcgg cgccgaaggc
     2161 cttcacgatc ttccgccgct ccaccgacat cgtctcgggc atcacgaggg tgagccggta
     2221 cccgcgggcg gcgcagacaa aggcgagggc gatcccggtg ttcccgctcg tgggctcgag
     2281 gatggtagtg tccttcccga tcaggttctc cttctccgcc gcctcgatca tggagaggcc
     2341 gatgcggtcc ttgacgctgt tcatggggtt gaaggactcg agctttgcca caacggtggc
     2401 ctccagtccc tcggtcagcc ggttgatccg gacgaggggc gtccctccga ccgtctccgt
     2461 gatgtctccg tagatctgcg acattccttt cctcgcgttc cgggccaggc gctatgtgcc
     2521 gatcccaggg ctctcctcct tattaccagt tgtcatggca ggggagttca caattgtgaa
     2581 ttgcaccagt acactcgtca tccctgttta taatgatatc tcccggcccc cgttcaacca
     2641 aaaaactggc gtgggatcac gccgggggag gcagggttgc ggagcgggat gagtttgccg
     2701 caagaccaag gtataaacgg tcatggaagt accttttcac aattgtgata attcaggccg
     2761 gtcctgcaga gtacagggac cggttcatcg gattctgacg ggatgctgat cttcatgcgt
     2821 atacaaccga tacgtggtaa gccgtccggg ctcctgtggg cgacggcgat ccttgtccct
     2881 gctctgttgt tcgcggtatt ctcggccggt tgcctctctc aggctggtcc cggtacgtcc
     2941 tcctccgtgg gagcggacac gggaaaaaag ataacgagca tcggttctat cattgagaat
     3001 cccaccgcct acgagaaccg cacggtgacc atctcgggga agattgtcct cgaatgcggt
     3061 tcgggctgct ggttcaacct cgatgacggc tcgggtacta tctacgtgga cctgaagcct
     3121 cacaacttca tcattcccca gctgggagga gaaaaggccg tggtcacggg gaccgtggcc
     3181 atccgcgaca acacgcccag gattttcggg gagaaggttg aggtgggcgg caaggtctac
     3241 ccatgaacca cctttccctc gcggtgaaga acctgagacg ccgccggggg cggacgcttc
     3301 tcaccatcac gggagtggcc atcgcggtcg cggtcctctt ctcccttctt gccttcgatg
     3361 ccggctacga acagtccctg aaccgggaga tgaagggcct cggagtccac ctcctcgcgg
     3421 tgcccaaggg atgcccgtac gaagcggcct cactcatcct ccacggcggg gtcatcccca
     3481 agtacctctc gggggatgac ctggcccggg tccgggccct ggacggtgtc gagctcgctg
     3541 ccccgatgct cctccagcag ttcgtgaaga acgggacccc tcacgtcgtg tacgggatcc
     3601 agggcagcga tatcccgggc ctgaagccgg gatggagtgt gaagggcagg ttcttctccg
     3661 atggggagag cagggtgatg ctcgtcggga gtgacctcgc cgcggaggag gggctggagg
     3721 tgggatctgt gctccctttc gggaaagacc gcgaaccctt caccgtcgtg gggatcctcg
     3781 aaaggaccgg gggccaggat gacaacttcc atttcgtgcc gctcgccgag gcccagagga
     3841 tcttcggtct ggaggggaag atcacggcta ttgcggtcag actgaaggac atcgggagca
     3901 tctcggatat ttccaccgaa atggagcaga tccccgacat ccaggtggtg accatggccc
     3961 aggtgcaggg gaccatcctt tccctggtgg gatcggcgcg gaccctcatc ctctcggtga
     4021 tcgtcgtcgc cat
//
LOCUS       DATGDI010000220         4246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_111980, whole genome shotgun sequence.
ACCESSION   DATGDI010000220 DATGDI010000000
VERSION     DATGDI010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111980"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VKO45_08215"
     CDS             <1..1210
                     /locus_tag="VKO45_08215"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038938.1"
                     /note="catalyzes the ATP-dependent carboxylation of a
                     covalently attached biotin and the transfer of the
                     carboxyl group to pyruvate forming oxaloacetate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HMA05895.1"
                     /translation="KLVESEGLIFIGPHWKTIEAMGSKIGSKRMMRKAGVPVLPGTEE
                     GTTMEKAAKVADEIGYPVIVKASGGGGGIGMNIVNTPSELGPALSGSMRIAQSAFGDA
                     TVFIEKYLVRPRHIEFQVLADSSGHTVHLYDRECSIQRRHQKLVEEAPCPIMTPELRE
                     KMSKSAVRVAKTAGYTNAGTVEFLYSGGNYFFMEMNTRLQVEHTITEFITGVDIVKQQ
                     IAIAAGHDIPFTQDEVSIRGHAIECRINAEDPLKNFQADPGKITRYRSPGGPGIRLDS
                     GIHMGYSIPVLYDSLIAKLTAWGSDRSEAIQRMRRAIYEYVILGVKTTLPLHHALMHH
                     PQFVKGNTHTHFLKEEGVLDTLERYVREEETRMQTLAASLRGGREVVAITAAVNAYLA
                     GQAKGKNPGK"
     gene            1215..1907
                     /locus_tag="VKO45_08220"
     CDS             1215..1907
                     /locus_tag="VKO45_08220"
                     /inference="COORDINATES: protein motif:HMM:NF018410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HMA05896.1"
                     /translation="MAEAWGDDSAPIFSEKPVPDRGLRLALPFLLLALHLLVLLLAFG
                     AGTMAFIATLMVIYELPPAGKETVIPAGILLGYPWWAVAASIALVDVEVGLFMVWNFD
                     LAERIPVLGPWIRRFIGGGREFLAGRPWLTGLYFFGIMLMVMVPFFGSGGVRGSIVGR
                     LLGMSRREVMGAIAAGALIGCFGIALGTLYLRQAFLESLFTGAAALLVLILAGIIVAW
                     IAKRFVPGVQKP"
     gene            2006..2557
                     /locus_tag="VKO45_08225"
     CDS             2006..2557
                     /locus_tag="VKO45_08225"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05897.1"
                     /translation="MAIACILLFLAGTVLFAGCTQPTTYPTTPVPTTVATAAPTTAAP
                     TPAPDSVKVTSNPRYGQILTDSSGMTLYYFARDARGAGRTACTGSCASTWPPFSTAAI
                     QVSAPLSAGDFATFTRSDGQMQVSYMGWPLYHYAGDKAAGDTNGYGILQLWYVMSPTG
                     VVTLTPTTTVPTTPPYSSGGGGY"
     gene            2618..3211
                     /locus_tag="VKO45_08230"
     CDS             2618..3211
                     /locus_tag="VKO45_08230"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05898.1"
                     /translation="MQRWVTAFILLSVAGAVLFAGCTQPATPAPAPVTPTPVPDTVRV
                     AANPQHGQILTDANERTLYYFARDSPGYGTTYCTGSCAATWPPFNATAIQVSPPLRAD
                     DFGVFGRSDGLIQVTYFGWPLYYYSGDRSPGDANGYGFNKLWYVMGTNGVVTLAPTTK
                     PPTPVPTTPPVYNYGGGYSGGGGGGGGGGSGGGGGGY"
     gene            3287..>4246
                     /locus_tag="VKO45_08235"
     CDS             3287..>4246
                     /locus_tag="VKO45_08235"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HMA05899.1"
                     /translation="MPVRLQLNDGTVREYPVVNPVKGGFPSITKCGAKDCEHLDCALD
                     LARNLKGTFQSLVWGTVALAVLMIIAFLMLNFLFGRGIASVDLPDLLLIIAPVILILV
                     VYFSSGKRLERHSAAVKELEEYTGYRTINGIPAHQILTAPAAVTGVKPVPPGTRPGIL
                     GEMFRTSLVTDLFGNTEYEFRLLQKEPFVHAVRFFAVLLAIFSVLSIITSNLMASFPV
                     FYSWTGRTLDSGPVSLFIHFLGLFAFSFLLVFLNGAWLHVWVFLFGGRSGMRETLRLV
                     LYGSMPALLIGWIPEVGSFIGGLLSLGLYYHGITVFHSLSHGRS"
BASE COUNT          716 a         1445 c         1279 g          806 t
ORIGIN      
        1 gaaactcgtg gagtccgagg ggctcatctt catcggcccc cactggaaga ccatcgaggc
       61 catggggagc aagatcggct cgaagaggat gatgaggaag gcgggggtgc ccgtcctccc
      121 ggggaccgag gaggggacca ccatggagaa ggccgcgaag gtggcagacg agatcggcta
      181 ccccgtgatc gtgaaggcct cggggggcgg agggggcata gggatgaaca tcgtgaacac
      241 cccgtccgag ctcggcccgg ccctctcggg ctccatgcgc atcgcccagt cggccttcgg
      301 ggatgccacg gtcttcatcg agaagtacct ggtccggccc cggcacatcg agttccaggt
      361 ccttgccgac tcctccggcc acaccgtcca cctctacgac cgggagtgtt ccatccagcg
      421 ccggcaccag aagctcgtgg aggaggcacc ctgccccatc atgaccccgg aattgcggga
      481 gaagatgtcg aagtccgccg tccgggtggc gaagaccgcg gggtacacga acgcggggac
      541 cgtcgagttc ctgtactccg gcgggaacta cttcttcatg gagatgaaca cccggctcca
      601 ggtggagcac accatcaccg agtttatcac gggggtggac atcgtgaagc agcagatcgc
      661 catcgccgcc ggccatgaca tccccttcac ccaggacgag gtctccatcc gggggcatgc
      721 catcgagtgc cggatcaatg ccgaggaccc cctgaagaac ttccaggccg acccggggaa
      781 gatcacccgg taccggtcgc cgggcgggcc cggcatccgg ctcgactcgg ggatccacat
      841 gggctactcc atcccggtcc tctacgactc cctcatcgcg aaactgacgg catggggatc
      901 cgatcgatcg gaagccatcc agcggatgcg acgggccatc tacgagtacg tcattctcgg
      961 ggtgaagacg acccttcccc tccaccatgc cctcatgcac cacccccagt tcgtgaaggg
     1021 gaacacccac acccacttcc tgaaggagga gggggtcctc gacaccctgg agcgctacgt
     1081 ccgggaggag gagacccgga tgcagaccct cgccgcctct ctccgggggg ggagggaggt
     1141 cgtcgccata accgccgccg tgaatgccta tctggccggc caggcgaagg gaaagaaccc
     1201 ggggaagtaa ccccatggct gaggcctggg gggatgactc agcccccatt ttttcggaga
     1261 aaccggttcc ggaccggggg ctccgtcttg ccctcccctt cctcctcctc gccctccacc
     1321 tgctcgttct tctccttgcc ttcggcgccg ggaccatggc ctttatcgcc accctcatgg
     1381 tcatctacga gctcccgccc gccgggaagg agacggtgat cccggccggc atcctcctcg
     1441 ggtacccctg gtgggcggtg gccgcctcca tcgctctcgt cgacgtcgag gtggggctct
     1501 tcatggtctg gaacttcgac ctcgccgaac gaatccccgt gctcggcccc tggatccggc
     1561 ggttcatcgg gggcgggagg gagttcctcg ccggcaggcc ctggctcacc gggctctact
     1621 ttttcgggat catgctcatg gtcatggtcc ccttcttcgg gagcggcggg gtccgcggct
     1681 ccatcgtggg gaggcttctc ggcatgagcc gccgggaggt gatgggggcc atcgcggccg
     1741 gtgctctcat cgggtgtttc gggatcgccc tcggtaccct gtatctccgg caggccttcc
     1801 tcgagagcct cttcaccggc gcggcggccc tcctcgtcct catccttgcc gggattatcg
     1861 tcgcctggat cgcgaagcgg ttcgttcccg gggtgcagaa accctgaatc ccgcaggtcc
     1921 ctccattttc tgaaaggtga gaacaggtat aaataggcga aaatccctgc cttccgcgga
     1981 caaaggacgt gtctctatga gaaacatggc aatcgcctgt atcctcctgt tcctcgcagg
     2041 aacggtcctc ttcgcgggct gcacccagcc caccacctat ccgacgaccc cggtcccgac
     2101 cacggtggcg accgccgccc cgaccacggc ggcgccgacc ccggcgcccg acagcgtcaa
     2161 ggttacctcc aacccgcggt acggccagat cctgaccgac tcgagcggga tgaccctgta
     2221 ctacttcgcc cgggatgccc ggggagccgg gaggaccgcc tgtacgggtt cgtgtgcctc
     2281 cacctggccg cccttctcaa ccgcggcgat ccaggtctcg gcgccgctct cggcggggga
     2341 cttcgcgacc ttcacccggt cggacggcca gatgcaggtc agctacatgg gatggcccct
     2401 ctaccactac gcgggtgaca aggccgccgg cgatacgaac gggtacggca tcctccagct
     2461 ctggtacgtg atgagcccga ccggggttgt gaccctcacc ccgaccacca ccgttccgac
     2521 cacgcccccc tactcgagcg gtggcggcgg gtactgaact cctccccatt tttccctcct
     2581 cttacaggcg agttctatgc accacgcctc caatcccatg cagagatggg taaccgcctt
     2641 catcctcctg tccgtcgcag gagcggtact ctttgcaggg tgcacccagc cggcgactcc
     2701 ggcccccgca ccggtcacgc cgaccccggt tcccgacacc gtcagggtgg ccgcgaaccc
     2761 gcagcacggc cagatcctca ccgatgcgaa cgagagaacg ctctattact tcgcccggga
     2821 cagccccggg tacgggacca cctactgcac cggttcctgc gccgcgacct ggcccccctt
     2881 caatgctacc gcgatccagg tctccccccc gctccgggcg gacgactttg gtgtgtttgg
     2941 ccgatctgac gggctgatac aggtcaccta cttcggatgg cccctgtatt actactcggg
     3001 tgacaggagc ccgggggatg ccaacgggta cggctttaat aagctctggt acgtgatggg
     3061 caccaacggt gttgtgaccc tcgccccgac cacgaagccc ccgaccccgg tcccgaccac
     3121 gccaccggtc tataattacg gcggcggtta cagtggcggt ggcggcggag gcggcggtgg
     3181 aggttccggt ggtggcgggg gagggtactg actcctcact ctttttcagc ctctcgggag
     3241 ggaaaggtta cagggaggga gatcttagga tcccttcagg aatcccatgc ctgtgcgact
     3301 ccagctgaat gacggaactg tcagagaata cccggtggtc aatccggtga aagggggctt
     3361 cccctctatc acaaaatgtg gggcgaagga ctgcgagcac ctggactgcg ccctcgacct
     3421 tgcccggaat ctgaagggga cgtttcagtc cctcgtgtgg ggcacggtgg ccctcgcggt
     3481 actcatgatc atcgcgttcc tcatgctcaa tttccttttc gggagaggaa tcgcatcggt
     3541 ggatcttccc gatcttctcc tgatcattgc tccggttatt ctcatccttg tggtttattt
     3601 ttcctcggga aagcgtctgg aaaggcacag cgccgccgta aaggaactgg aggaatacac
     3661 cgggtaccgg acgatcaatg gcattcccgc gcaccagatc ctcacggccc cggcagccgt
     3721 gacaggggtg aagccggtac ccccggggac ccgaccaggc atcctcgggg agatgttcag
     3781 gacgagcctc gtcaccgacc tcttcgggaa caccgagtac gagtttcggc tgctccagaa
     3841 ggagccgttc gtccatgccg tcaggttctt cgcggtcctc cttgccattt tctcggtcct
     3901 ttccatcatc acctcaaatc tcatggcctc cttcccggtt ttctactcct ggacgggaag
     3961 gaccctggac tccggtccgg tctctctctt cattcatttc ctgggactct tcgccttcag
     4021 ctttcttctc gtcttcctga acggggcctg gctccatgtc tgggtcttcc tcttcggggg
     4081 aaggagcggg atgcgggaga ctctccggct cgtcctctac ggatccatgc ccgcactcct
     4141 catcggctgg atccccgagg tcggatcctt catcgggggg ctgctcagcc tcgggctgta
     4201 ctaccacggg atcaccgtct tccactccct gtcccacggg aggtcc
//
LOCUS       DATGDI010000221        26931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_373461, whole genome shotgun sequence.
ACCESSION   DATGDI010000221 DATGDI010000000
VERSION     DATGDI010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 26931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26931
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373461"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..423
                     /locus_tag="VKO45_08240"
     CDS             262..423
                     /locus_tag="VKO45_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05900.1"
                     /translation="MANLEKLTDGELRALMRSRYHFLGSYMMTRVREWDICRDDSVDW
                     MIRKGGDVL"
     gene            547..1194
                     /gene="hypB"
                     /locus_tag="VKO45_08245"
     CDS             547..1194
                     /gene="hypB"
                     /locus_tag="VKO45_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HMA05901.1"
                     /translation="MHHIDLRIEKDIYGANNRIADANAAHLREHGVRAFDLLGAIGSG
                     KTALIERMVPLLVARGLRAGAIAGDVYGDDDFRRIVALGIPAVNANTGKECHLDAHLV
                     EHAIEDLPLDRIDLLFIENVGNMVCPTDFRLGAEKRIVIVSSTEGDDVVNKHPMIFRD
                     GTIGVINKVDLAPLVGANLDRMETDMHRYNPKMPVFRTNLKTGEGVGPLLEAILS"
     gene            1272..1649
                     /locus_tag="VKO45_08250"
     CDS             1272..1649
                     /locus_tag="VKO45_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HMA05902.1"
                     /translation="MLWQGRSVRRESGKRYRISRRKKRFEIGRAAADTTLGEVRTRLI
                     RTRGGNLKVRALRTQFANVSVPATGQTRKAKIETVVENRADPNYMRRNILTRGAIIKT
                     ELGKARVLSRPAQDGVVNAVLIE"
     gene            1748..2170
                     /locus_tag="VKO45_08255"
     CDS             1748..2170
                     /locus_tag="VKO45_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05903.1"
                     /translation="MRRIYYRFPNTYDLDFDLNRPFPEVLGCIRRLHDTHQTSRRGKD
                     LVKRQILVQLATNKMSFEMVDSLPPFEETVTEMADFRFILHDILLAARGRVPLGENIF
                     LMRVQDKGPLSECIVVKEGQQGAMDAAEFKTLIKGACP"
     gene            2167..2640
                     /locus_tag="VKO45_08260"
     CDS             2167..2640
                     /locus_tag="VKO45_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2240 family protein"
                     /protein_id="HMA05904.1"
                     /translation="MSLAVTVAAPFKHTRKDRLQKSEFIFYLTIDRKWMSRDQANQVL
                     DRAKDAGMVEVADGWVRPLLDLSSVTVPVGFRPDSRIFESEEASQVLIGRIATARGVP
                     PAEVVAEVNRVIREGFDGNLRLPAAAVIVARRHGIGFTDLLDRLMVEVLEKKDGK"
     gene            complement(2647..3090)
                     /locus_tag="VKO45_08265"
     CDS             complement(2647..3090)
                     /locus_tag="VKO45_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6e"
                     /protein_id="HMA05905.1"
                     /translation="MVDFKLVLSDAKAGRAYNVSVSGGSANAFLGKRIGDEVDGAPVG
                     LGGYRMRISGGSDRNGNPMRADLPGMARRRLLLSDGTGFHSRLEGERRRKMTRGNEVT
                     PDLVQINAVVTAYGEKPLGEYFKKGEEKPAEKEGQAPKERPAKKK"
     gene            complement(3242..5026)
                     /gene="infB"
                     /locus_tag="VKO45_08270"
     CDS             complement(3242..5026)
                     /gene="infB"
                     /locus_tag="VKO45_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HMA05906.1"
                     /translation="MAAPAPAGIRTPIVCVLGHVDHGKTSLLDRIRGSSVVESEEGAI
                     TQHIGATLVPLEVIRAMSGTLGKLDIQVPGLLFIDTPGHHAFTTLRARGGALAEMAIL
                     VVDINEGFQPQTLEALEILRTYKTPFVVAATKIDRIHGWRVNENETFQASFGKQGERS
                     RTALENKTYEIVGRLSELGFASERYDRISDFQRNIAIVPVSSHTGEGIADLLMILIGL
                     AQRFLKEDLAFRADGPARGTVLEVKEERGLGMTLDVILYDGTLSVGDEVMLATANGVV
                     TTKVRSLLKPRPLREIRVEDRFDRARSVVAAAGIKVAAPGLEAVVAGSPLSAVRGDRE
                     AVEATIRHEMEDIRVTLSDRGITLRADTIGALEALSKELEGKSIGILRAGVGPVSRHD
                     IVEVQTVADPLSSVLLCFNTPILPDAQDLLKDPAFSRVKVFSGKVIYQLINEYLEWRD
                     AEIRKREAQRFEEVVLPARILILPNCVFRQSNPAVVGIRVLLGKLRTGVELADRSGRR
                     IGHLKTMQSRQENIREADTGAEVAISIEGATVGRQVNVNDELFVEIPERHVKILEHEM
                     LPHLTPSMRECLEDYTAIMRKDNPFWGK"
     gene            5251..5323
                     /locus_tag="VKO45_08275"
     tRNA            5251..5323
                     /locus_tag="VKO45_08275"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5285..5287,aa:His,seq:gtg)
     gene            complement(5417..5773)
                     /locus_tag="VKO45_08280"
     CDS             complement(5417..5773)
                     /locus_tag="VKO45_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HMA05907.1"
                     /translation="MPEDVIQPTTASPSNLTELFVFEQPARRLELLVRILYWIIIGLV
                     LMVYGIIATACLIIQWFVILILGHRNRGLSDFIQGYLEYRIHVTPYSAFMTDRRPDLN
                     PHKVRIFEEYPVGEKK"
     gene            complement(5878..7251)
                     /locus_tag="VKO45_08285"
     CDS             complement(5878..7251)
                     /locus_tag="VKO45_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMA05908.1"
                     /translation="MESVKDLEDFGVIKRLLRDEPRGMTVTELARALGRTKNTVGRYL
                     DILHASGQVEMRSFGMAKVFTLTQRVPLPQLFSHTAEMVMILDRDRRVLQVNDPFLSL
                     LSLGRDDVVGKNLAYLPVPEPGVQDMVRALLASLEKESTDAGLEISRGGERYLRAKVI
                     PILFEDGSQGSTVILADETPVRLAEKALRESEKKYRELVEKANTIILKMDLAGGVTFF
                     NEYAEGFFGFSRAEILGKNVIGTIVPPTESSGRDLEQMIARLLSHTEEYESNENENIT
                     KEGRRVWVRWSNQAIRDEQGNPVGVLSVGSDITEHRRLDEELRRERDFIGATLHILDA
                     LVVVLERDGRIMWFNHACEALTGYAEEEVKGRPFWEIFLLPEERDRVREGFDGLVAGQ
                     GQLRLSNFWVTRTGERKYIRWSNTVLRDEKGSTTHVIGTGIDITEHRRLEEELGKYRT
                     GETLPGG"
     gene            7326..8666
                     /locus_tag="VKO45_08290"
     CDS             7326..8666
                     /locus_tag="VKO45_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05909.1"
                     /translation="MYKQTTDQGHMMISMHAHPVKGRAASRGAIPWICLVLILAAVPL
                     AAGASTTDPTVAITGYHVTPSILLPGDQGTIEITIANTASTASTTESTVQNGVSGSTT
                     TTTTKDMTVLVESVYMYGQGVEVLEGNFQQVGALGPGQSMTLTFLIKAPPQAGMYFPE
                     VWIRIPEGTSVKYPIPVNVNSPIGIQKQAVLVLSSFTPDSVNPGEEVPVTLSVRNDGD
                     LLAEDVAVRIGNVSTLVAPKGTDLYHLGLVGSGRTKSAELVLLSDRDTSPGLTQVPVT
                     LQYSSVDGVVHTQASSINLMMKGKSELGFVSVDTSPRRVGENQPFDLTIRIENTGTGE
                     AKQVAATIDLPMTGTKQSFIGKIKPGNDAPAVFLLDGAKGGSYAYNATISYVDDLGSH
                     TEVRQMSVRIDPADSSGGFILLLLVFIGGGLLAYRFWYIPRKNGGGALPWVRKS"
     gene            8648..9862
                     /locus_tag="VKO45_08295"
     CDS             8648..9862
                     /locus_tag="VKO45_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA05910.1"
                     /translation="MGQKELKVSLFLAWRSLIRGNRSSTVMTVIIIALCFTNMIFLPG
                     LFNGIGQSITAQIVDYEVGNVLVSPRAGDQYITDLHATLSLINSLPGVERATPHYTKG
                     ATLKSRDRILGVGVRAISPSDEKYVSPLYTKMIAGTYLGDGDTGEIIMGKTVAGDASV
                     RQEDEFQPSLGGVRVGDSITIEYGNGYTKDYRVKGIFFTGWSQADSTVYTTMSDMELV
                     EGKALDQADYITVKAMPGYSEKSIKNELQSYGVSQDVQTTSDLLAKGMGRVLQSFAII
                     NLVSLIVSIIITTVVLFIVITIKTINSRKQIGILKAIGVDKTVIMHSYGFQVIILALF
                     GIIIGVIITSLLVVYMSIHPIVTPEWSAYLFLTPLDMLTNSLILFGAAVVAGYVPAWR
                     VALEDIQKTMRA"
     gene            9867..10544
                     /locus_tag="VKO45_08300"
     CDS             9867..10544
                     /locus_tag="VKO45_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA05911.1"
                     /translation="MIRTENLRKVYRMGNVEVKALDGVDIHIEKGEFVGIIGPSGSGK
                     STLLHMLGLLDIPTAGSIFIDGTDVGTLTDYQKTMFRLYKLGYVFQDYALVPELTVRE
                     NVALPAMLRADRSEAQYKADTVDILTKVGLCDRRTHLQSELSGGQQQRVAIARAIVNK
                     PDILFADEPCANLDTENSRMVLNLFHEINRSMKQTIVMVSHEEWHKEYFHRIIRLRDG
                     KIEDDGK"
     gene            10703..12859
                     /locus_tag="VKO45_08305"
     CDS             10703..12859
                     /locus_tag="VKO45_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMA05912.1"
                     /translation="MISVLHVDDEQDFLDLTRAFLEKSGDIRLEGVTSAESALERLEE
                     TRYDAIISDFRMPGMDGIGLLKHLRESGNRIPFIILTGRGGEQVAIEALNNGADFYLQ
                     KGTDPRVLFIELRNLIGQAVTRRQAVEALKKSEEWYRNLTEASQDLIYIIDRDDNVVY
                     LNRHSQELLGKKTEEVVGRPRSLLFPPDADEHMKRNLATVFSDSHPIRVESLIPVGTQ
                     EIWLDTTLIPLRSAEERVTWAMGISRDITERKRIEDALRESEEKYRNLVDRAYEGIIV
                     VQDDVVKLCNRRTADMWGGECRDITGRPYRDFIDPSELPRLRRNYELRMAGKKVPGRY
                     DTRLLRRDGTSFPVDLEGGDIVFDGRPADLIMFRDITERKKAEVALRESEEKYRALVE
                     RANDGIIVVQDGIVKFCNRRAAELWGGDVGEIEGRPLQDFIDPVDVSRIRENYERRMA
                     GEEVPRQYDALLLRKDGSRFHGELNAGAFTFEGRAADLVLFRDMTERKKAEEALKNVN
                     RKLNLLSSVTRHDMLNQLVAVVGYLELALESPSPGDARELAGMARKAAVTMRHQIEFT
                     RQYQEVGVHSPVWQDLREVIARAASDAGNGRVRVEIDLDPVRIYADPLLERVFSNLIE
                     NAILHGQKVTRIRFSVRATDPGLAILCEDDGIGIPAHDKERIFAHGYGRKTGYGLFLV
                     REILAITGATVREIGQPGEGAVFEITVPRDAYRAGG"
     gene            12884..13336
                     /locus_tag="VKO45_08310"
     CDS             12884..13336
                     /locus_tag="VKO45_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05913.1"
                     /translation="MCGTCCMSMGDCIAVEREIGPFEFECESISTGTPFRAIVDEDKR
                     ELFRDITWIKEHPRACQFLRPKGEQVLCTIHGTSPAQCKYYRCVVMRIFEPGGDLAGR
                     VTGTLALHSDNPVLREIWEAALREIPEGAGDVELRLQKVLESHGCRVE"
     gene            complement(13396..13563)
                     /locus_tag="VKO45_08315"
     CDS             complement(13396..13563)
                     /locus_tag="VKO45_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HMA05914.1"
                     /translation="MYRSDFGGRRNFGPREMTKVICSDCGKECEVPFKPTEGRPVYCQ
                     ECLPKHRRPRF"
     gene            complement(13648..13986)
                     /gene="eif1A"
                     /locus_tag="VKO45_08320"
     CDS             complement(13648..13986)
                     /gene="eif1A"
                     /locus_tag="VKO45_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HMA05915.1"
                     /translation="MPGNPKKNNDAVDPNAVDAQGQPIVRVRLPKKWNREQFASAELM
                     MGANHVRVRCYDGVTRMGRIKGKIKKRVWIREGDILIVTPWSFQDEKCDILYRYLPPQ
                     VEWLRRNRYI"
     gene            complement(14045..15127)
                     /locus_tag="VKO45_08325"
     CDS             complement(14045..15127)
                     /locus_tag="VKO45_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA05916.1"
                     /translation="MKGRHIWIVARKELSGINYEKTILFAILLQLFVAMFSSFLMVGL
                     TSLYDPSSVSQYSRFRYSIAYTGANSTLLGRLQDSDDFTVFEMDLSPAIESLKERKLA
                     AVLFVPDIPPDSPDPVQVTLYTLQNDLQATVVGVKLKEVLLAFEGDLRTARAARISAQ
                     PIELAFPPAAPAEGFYEFVYGLLIPLLMFMPAIISASLVIDLITEEYQHETLETLIST
                     PVTFAEMVWGKILACEILVPVQAAAWLLLLAVNGIRISNPVPILLLVSAASLILILLS
                     ALTALHYRERTAAQFIYSTALVIVILLVVAIPGNPLNLIVRASIGDPGPGIYGALLLV
                     PAAAALLGYITHLYASRIGMLAQGGA"
     gene            complement(15124..16878)
                     /locus_tag="VKO45_08330"
     CDS             complement(15124..16878)
                     /locus_tag="VKO45_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA05917.1"
                     /translation="MGRQVLPLAVVLFILLILSTGFVARSGMHLQDGMYTLATDDREL
                     GAILAADARFTVFIGDGATLPSQRAAFDLVVLNRTVLPPLSEKGASALKTLGRDYSQY
                     VASVYNREPDLFAAYPLWIDLQYVRSELDFQATQQGQYISVRQRSSAPPVPEGPVVPV
                     TPPAATLPVSADDLRRNLQQEAGKNDQVARYTGMFTGLPSPNSSVRTPSQLSPSLPFD
                     SIIFIFVFIFPLYFTSQFFMMSVMNERIERKGEILLSGPLSPVVIIAGKMLPYLARMI
                     FTSSVVILTARASLLILLPLLPVILFFLASALLIGMLSRSFKELSFISIFFSTLATSY
                     LFFPSIFANVHIISLVSPLTLIILQIQGTGFTATDYLYSTSLFYLTSGVLLFIGVANF
                     NAERLFDLRPFLPRLREFVTSSLSREHPWRSLLVANILLIPFVFMAQMMGLVLLFNLP
                     MPLSLVLLILLAALIEEIAKSVGVYSLFHAELSGLPWRTVILASAVTAAGFLLGEKLF
                     LLAMLTQITDSVFGSVLFLQQGILLLWMPFLLHFTGVLIVILVLRTGGRRWYLPGILA
                     AAVVHSLYNLSLLSGVLG"
     gene            17021..17908
                     /locus_tag="VKO45_08335"
     CDS             17021..17908
                     /locus_tag="VKO45_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA05918.1"
                     /translation="MISACGLSKTYDGFPALDGVGLALGDNEILGVIGHNGAGKTTLL
                     KIMTGLVPPTGGDLTINGIDVVEDPIGLKKILGYLPEDSHLYETMTVRSYLTFFGEIY
                     GMGREAIRERADRLLATLSLEDGGKKIGELSKGMKRKVAIARSLLHDPAVLIYDEPSS
                     GLDPMTSRTIIEFLKELRGQGKTIVFSAHNLYQVEEVCDRVMILRRGKEVAYGSIPEL
                     REMFGSITYTLTFSIADLSRLGNHRTYRKDGVYYAVDGLDIEGMNELATSLPASGGRV
                     ERVESHYPTLEQMLVKIGN"
     gene            complement(18089..18727)
                     /locus_tag="VKO45_08340"
     CDS             complement(18089..18727)
                     /locus_tag="VKO45_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05919.1"
                     /translation="MEPVAVAFAVVVVVAMIILLGGVYLRTTQPEPVPTPAPADTGVT
                     EIPTPVETTLAETIPPTPEPTIEIPSLPPTKDMSRAWVQPATPFHDKKYYLLPYHQTV
                     YDPRATGLPPVIFQQSYNLDFQDEAVVVNVPRPPLILDYALTRFISPTRTFVYITIRD
                     NQTQQLLSQEGFYGPYSTNSPKRLFFSSPGTYHINMYGGFSGIGLTIRAHPS"
     gene            complement(19014..19087)
                     /locus_tag="VKO45_08345"
     tRNA            complement(19014..19087)
                     /locus_tag="VKO45_08345"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19051..19053),aa:Lys,seq:ctt)
     gene            19333..20808
                     /locus_tag="VKO45_08350"
     CDS             19333..20808
                     /locus_tag="VKO45_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HMA05920.1"
                     /translation="MAGRDAEKGKKKVKYLIFGCGSTGYNVVEALLRETPDILIIDRD
                     EKRVEDLRVQKFNALVRDLTSPALVNGLPEPDVALVLSNDKDANLAAVRTIRSAYPKA
                     HIIARATDPVSIGMLQEAGGDLVLYPQEVVAREVEGHLLQLHSSRLARRLFELIAGWE
                     GTLGIVTHTNPDPDSISSAMALVAIAAEANPKNLTCRILYGGNIGHQENRAFVNLLDI
                     NMERITPQVLKECDYLALVDSLGPGMNNDLTKKTQVNIVIDHHTNGEIPEVKGDFVDI
                     RTGIGATASMMAQYLKELGIVVNKKVATALLYGIQADTRYFRRNVTPADFVYAGFLLP
                     FTDLELLEKITSPAVSQETLDVLGAAIRNRKIRSGYLFSNVGYVRNRDALPQAADLLI
                     NLEGVNTALVFGIGDSAIYISARNKDIRLHIGNVLAEAFGDMGEAGGHPNMAAANIPL
                     TYFSLVKTKDDLLTLVLEPIMRKFMRVVGLEKEESHEIFEI"
     gene            20885..21535
                     /locus_tag="VKO45_08355"
     CDS             20885..21535
                     /locus_tag="VKO45_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HMA05921.1"
                     /translation="MKSRKNGLIFAGFEDWEPYYLRILEHFHFDRARDEEAALLLASL
                     LARDDLPVADALIRGKTATVCGNGPNLTDELDRIRGTVIAADGAANRLFARGIPPGVI
                     VTDLDGATDAFIRMNVAGTVIVVHAHGDNMPLLRSWVPRFPGPLVGTTQSRPFGRIHN
                     FGGFSDGDRAVFLAHALGASEVAIAGFNLDDPNVDPVKREKLLFARELLGLAGHDL"
     gene            21525..23174
                     /locus_tag="VKO45_08360"
     CDS             21525..23174
                     /locus_tag="VKO45_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA05922.1"
                     /translation="MTSDAVILDGYIDEPACLGVPPYIAPQPRSLAGVLREHGYRVRY
                     HTIDQLRQDPSLLLSLDTADIAVMVAGVTVPGKYLAGTPATLTEVEQVGATLRHPKTF
                     LAGPLLFGYSPRGGERAKRREIAGFAVILEGSPAGALDSFLRGGEPRSDGDYRQMAGW
                     MVAGAPVIRQHPSFPHLMCELETATGCSRAVSGGCSFCTEPFYGPPRFRTAGEIVAEV
                     SALHANGALHFRLGRQPDLLAYGVSGGEFPRPDPEKIRDLFTGIRQAAPDLLTLHIDN
                     VNPGTIARHEEASREALRAIIGGHTPGDVAAFGMETADPAVIAANNLKATPEQVTQAI
                     RVVNEEGGRREEGIPGLLPGLNFVIGLAGETEETFRLNREFLSRVLAEGLLVRRVNIR
                     RLMAFPGTRAFDVNTLGKHDRFFRAFKEGVRRDFDHPMISRVFPLGTILRRVVVEVEG
                     RISYGRQMGSYPVLVGIPLPVQVRTLLDTVVVDWGMRSITALPFPIEINRLPVRALSW
                     IPGIGKKKAAALAATRPVRDLASFRSVAGETPLEGALSFVP"
     gene            complement(23171..24307)
                     /locus_tag="VKO45_08365"
     CDS             complement(23171..24307)
                     /locus_tag="VKO45_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA05923.1"
                     /translation="MASSIKIGSLFGIPVLLHWSFLLAIPLFAWIIGTDIAISASFVF
                     GIFGVPGANTSLITQGWISYILGAIVALALFFCVLVHELAHCVVALRRGIKINSITLL
                     ILGGVSSIENELPDPRDELPMSLAGPVTSAGLGILSWIVVIAGAPFISDLPVKGVVIF
                     LFGYLALLNIILFAFNLLPAFPMDGGRVLRAWLARRMPLPRATKIAADIGKAFALIFV
                     VFGLFTLNFILIIIGIFIYLGADQEFYAVRSTVLLRDVAVKDVMSTNLVTVEPQLPVP
                     EVVRLMYATKHLGFPVLENGVPVGMVTLEDVHRVSEIDRDAKQVKDIMARNLITVPPT
                     AMLSEAFRLMSRYNVGRIPIIENGRLLGMVTRTDILKVMELREA"
     gene            24359..24610
                     /locus_tag="VKO45_08370"
     CDS             24359..24610
                     /locus_tag="VKO45_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HMA05924.1"
                     /translation="MITQITELFGLSVYTDKAIYVGDVEDVVIDVDNKKLDALAIGNL
                     NPELVDVKGFKGIKIPFRIIKAIGDIIVIRHLSPSFRQE"
     gene            24677..25414
                     /locus_tag="VKO45_08375"
     CDS             24677..25414
                     /locus_tag="VKO45_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008193 - tRNA guanylyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HMA05925.1"
                     /translation="MQEREIFGNLAIVPPIFVRLDGRAFHRLAKDLSLERPFDEGFSR
                     AMAGVSSRLLTESGLSPLFAFTFSDEISLYFPALPFRGRVEKIDSVLASFAASCLTLA
                     LSPGHPLAFDARVIIASPGYAREYLAGRQREAWRNHLNAYCQEALTMEGMKPTEVARR
                     LSGMRGEELHEFMFSRGVNLAKTPAWQRRGVLVYRRGEERAGYNPVKGESVETIRRVP
                     TVDRDLPVFTSPEGQAYLDGILGPSGP"
     gene            complement(25403..>26931)
                     /locus_tag="VKO45_08380"
     CDS             complement(25403..>26931)
                     /locus_tag="VKO45_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HMA05926.1"
                     /translation="DQTRGWFYSQLGASTIAFSRSPYRSVLMHGFALDAEGRKMSKSL
                     GNVVAPQEVIEKVGVDVLRLYILSANAPWDDLKFNWDGVKTVNRAVNILWNVYRFPLP
                     YMILDGYKPPEDREGRFDPDRILNGIPAMPPEDRWILSRVATLSGEVESSMEVCELHR
                     ATRAILDFILGDLSRWYVQLVRPRMWLEEDSPSKRRAYDTIYLVMRRLVQLLSPFAPH
                     LAEEIYGNLRLSGEPVSVHMLDWPSVDPELADRDLETGMAVVRAFDDAVANARQAAKR
                     KLRWPVAEVVVETASPPVAGALRDLGEIARTRANSKRLTLVEGSWDRMGIRAEPVMRT
                     VGPAFGKDAPRVKALIEGADARSLREGLERDGKFPLTGGGRSFEVTPAHVSFARVIPD
                     GFTSAPMEGAVVYVDGRLTPELEGEGYAREIIRRIQEMRRQLDLAVEDFIVAGVVIHD
                     PRVASLVRSGWENRIRDEVRARSLSIRLPGEGGPSPEGGLEADWEIEGVGVRTTVSRA
                     "
BASE COUNT         5286 a         8194 c         8740 g         4711 t
ORIGIN      
        1 atattgacgg tgaacctata aacaggtttc atgcagtgaa accttgttcc ctaccacgcc
       61 accccgtcag ggatatcagg gccaggtggc cggttggcag caatgggccc ccgagacatt
      121 aatcaggtct caatacaggg aggaaaacca taattaatat attatatatg cttatttcat
      181 taacagacaa gaatatgtac tgaatacaaa tagaaggtta taaatataaa taatgcaaac
      241 taaaagatag ggggcttcag catggccaac ctcgaaaaac tgacggatgg cgaactccgt
      301 gccctcatgc gttcacggta tcatttcctg ggatcctaca tgatgacccg ggtccgcgag
      361 tgggacatct gccgggacga ctccgtggac tggatgatcc ggaagggcgg ggacgtactc
      421 tgaaccggta aaatcctttt cttgcataag gggtcgggga taaccgcctc attttttctt
      481 gactcctgaa accctggcat tgcctccaac gatagataca aacagcatca ggatccatag
      541 gtgagcatgc accacatcga tctccggatc gagaaggaca tctacggggc caataaccgc
      601 atcgccgatg ccaacgcggc ccacctgagg gagcatggcg tgcgggcctt cgatctcctg
      661 ggtgccatcg gttcggggaa gacggccctc atcgaacgga tggtccccct gctcgtcgcg
      721 cgggggctcc gggccggggc catcgcgggc gacgtgtacg gggacgatga cttccggcgt
      781 atcgtggccc tcgggatccc cgcggtgaac gcgaacaccg ggaaggaatg ccacctggac
      841 gcccatctcg tggagcatgc catcgaggac ctgcccctcg accggatcga cctcctcttc
      901 atcgagaacg tcgggaacat ggtctgcccg acggacttcc ggctgggggc agagaagcgg
      961 atcgtgatcg tctcctccac cgagggggac gacgtggtga acaagcatcc catgatcttc
     1021 cgggacggga ccatcggggt gatcaacaag gtggacctcg cacccctcgt gggggcgaac
     1081 ctggaccgga tggagacgga tatgcaccgg tacaacccga agatgccggt cttccggacg
     1141 aacctgaaga ccggcgaggg ggtcgggccg ctcctcgagg ccatcctctc ctgaccaaag
     1201 ggtttaataa tctcggtccc catatttatg aacccagacg tgaaattcaa agacgcggga
     1261 gggtgattct catactctgg cagggaagat cggttcggag ggagagcggg aagcggtacc
     1321 gcatctcccg gcggaagaag cggttcgaga tcgggagggc ggcggcggac accaccctcg
     1381 gggaggtccg gacccggctc atccggacga ggggcggaaa cctcaaggtc cgggcgctca
     1441 ggacgcagtt tgccaacgtc tcggtgcccg ccaccggcca gacgaggaag gcgaagatcg
     1501 aaacggtggt agagaaccgg gccgacccca actacatgag gcggaacatc ctcacccgcg
     1561 gggccatcat caagaccgag ctcgggaagg cccgggtgct ctcccggccg gcccaggacg
     1621 gggtcgtgaa cgccgtcctt attgaataag aaggacccgg cgacttccct cgaggaaggc
     1681 gcacgccgtc cttctcgagt aaaacaaccc atcctttttt accctccgga acgctcatac
     1741 ctacttgatg cgcagaatct attaccggtt cccgaacacc tatgaccttg acttcgacct
     1801 gaaccgcccc tttcccgagg ttctgggctg catccggagg ctccacgaca cccaccagac
     1861 gagccggcgg gggaaggatc tcgtgaagcg ccagatcctc gtccagctcg ccacgaacaa
     1921 gatgtccttc gagatggtgg actccctccc ccccttcgag gagacggtca ccgagatggc
     1981 cgacttccgg ttcatcctcc atgacattct cctcgccgcc cggggccggg ttcccctcgg
     2041 tgaaaacatc ttcctcatgc gggtgcagga caagggcccc ctttccgagt gcatcgtcgt
     2101 gaaggagggc cagcaggggg cgatggatgc ggccgagttc aagaccctga tcaagggtgc
     2161 ctgcccgtga gcctcgccgt caccgtcgcc gcccccttca agcacacgag gaaggaccgg
     2221 ctccagaagt cggagttcat cttctacctc accatcgacc ggaagtggat gagccgggac
     2281 caggcgaacc aggtcctgga ccgggcgaag gacgccggga tggtggaggt ggccgacggc
     2341 tgggtccgcc ccctcctcga cctctcctcg gtcacggtgc cggtgggctt caggcccgat
     2401 tcccggatct tcgagagcga ggaggcctcc caggtcctca tcggccggat cgccacggcc
     2461 cgcggggttc cccccgccga ggtggttgcc gaggtgaacc gggtcatccg ggagggattc
     2521 gacgggaacc tgcgcctccc ggcggcggcg gtcatcgtgg cccggaggca cgggatcggg
     2581 tttaccgatc tccttgaccg gctgatggtc gaggtgctcg aaaaaaagga tgggaaataa
     2641 tacaatttac ttcttcttcg caggccgctc cttcggggcc tgcccttcct tctcggcggg
     2701 cttctcctcg cccttcttga agtactcgcc gagaggcttc tccccgtacg cggtgacgac
     2761 ggcgtttatc tggacgagat ccggggtaac ctcgttcccc cgggtcatct tccgccggcg
     2821 ctcgccctcg agccgggagt ggaaaccggt cccgtcggag agaaggagcc gcctcctcgc
     2881 catgcccggg aggtccgccc gcatggggtt cccgttccgg tccgatcccc ccgagatccg
     2941 catcctgtac ccgccgaggc cgacgggggc cccgtccacc tcatccccga tccgcttccc
     3001 gaggaaggcg ttcgcagaac cgccgctcac gctcacgttg tatgcccgcc cggccttcgc
     3061 gtccgagaga accagcttga agtccaccat tcgtcctgcc ctctggagtg acaatatcat
     3121 tggcccggag ggttcttaaa catagtgccc ggcccacctg aagaccggac ccacgccccc
     3181 gcggagaccg ggacccccgg gatcctcccg gaccatgccg ccggtgcgaa gcgcggaagg
     3241 atcacttccc ccagaagggg ttgtccttcc tcatgatcgc cgtgtaatcc tcgaggcact
     3301 ccctcatcga cggcgtgagg tgggggagca tctcgtgctc gaggatcttc acgtgacgct
     3361 cggggatctc cacgaagagc tcgtcgttca cgttcacctg cctccccacg gtggcaccct
     3421 cgatggagat ggccacctct gccccggtgt cggcctcacg gatgttctcc tgccggctct
     3481 gcatcgtctt caggtggccg atccgcctcc cgctccggtc ggcgagctcg accccggtcc
     3541 tcagcttccc gaggagcacc cggatcccca ccaccgcggg gttgctctgc cggaagacgc
     3601 agttcgggag gatcaggatc cgagcaggga ggaccacctc ctcgaaccgc tgggcctccc
     3661 tcttccggat ctcggcgtcc cgccactcca ggtactcgtt gatgagctgg tagatgacct
     3721 tcccggaaaa gaccttcaca cgggagaagg cggggtcctt gaggagatcc tgggcatcgg
     3781 ggaggatggg ggtgttgaag cagaggagga ccgaggagag cgggtccgcc acggtctgga
     3841 cctcgacgat gtcgtgccgg gagacggggc cgacgccggc tctcaggatc ccgatggact
     3901 tcccctcgag ctccttggac agggcctcga gggccccgat ggtgtcggcc cggagggtga
     3961 tcccccggtc ggaaagggtg acccggatgt cctccatctc gtgccggatg gtggcctcca
     4021 ccgcctcccg gtcgcctcgg accgccgaga ggggagaacc ggcgaccacc gcctcgagac
     4081 ccggggccgc gaccttgatc ccggcggccg cgacgacgga cctggcccgg tcgaaccggt
     4141 cctcgacccg gatctcccgg aggggcctcg gcttgaggag agaccgaacc ttcgtcgtga
     4201 caaccccgtt cgccgtggcg agcatcacct cgtcccccac cgagagggtc ccgtcataga
     4261 ggatcacgtc cagggtcatc ccgaggccgc gttcctcctt cacctcgagg accgtccccc
     4321 gggccgggcc atccgcccgg aaggcaaggt cctccttcag gaaccgctgg gcgaggccga
     4381 tgaggatcat gaggaggtcg gcgatcccct cgccggtgtg cgaggagacc gggacgatgg
     4441 cgatgttccg ctggaagtcg ctgatccggt cgtaccgctc cgaggcgaag ccgagctccg
     4501 agagtctccc gacgatctcg taggtcttgt tctcgagggc cgtgcgggac cgctccccct
     4561 gctttccgaa gctcgcctgg aaggtctcgt tctcgttcac ccgccagccg tggatccggt
     4621 cgatcttcgt cgccgcgacg acaaaggggg tcttgtaggt ccggaggatc tcgagggcct
     4681 cgagggtctg gggctggaat ccctcgttga tgtccacgac gagaatggcc atctcggcga
     4741 gggctcctcc ccgggcccgg agggtggtga aggcatggtg gccgggagtg tcgatgaaaa
     4801 ggaggccggg gacctggatg tcgagcttgc ccagggtccc gctcatggcc cggatgacct
     4861 ccagggggac gagggtggcc ccgatgtgct gggtgatggc cccctcctcg ctctcgacga
     4921 ccgaggaacc ccttatccgg tcgaggaggg aggtctttcc atggtccacg tggccgagga
     4981 cgcagacgat gggtgtcctg atgcctgcgg gtgcgggtgc tgccatgtgc cgacctccat
     5041 cgtaatcctc ggtgagaagt gagaagagta tcattcacgg tatcataaac ctgcgtattc
     5101 cccccctctc atcttcacgc accgaggggg caggagaggc atatttttat cagattcacc
     5161 ggcaaatggt ggtgaatggt gttcaatgca gcctcgggct gcgtgaacgc cgttcgagaa
     5221 gatctccgag gagatcttcg aggtacattt gccggggtgg ggtagttggt tatcctaggg
     5281 gactgtggat cccccgaccc gggttcgaat ctcggccccg gcctctgaaa ttctcatgaa
     5341 aattgagaga ctttctgttt tccaggcact gcccagacat ccccttgaaa aaaggttcag
     5401 ccgggaaaat tttcggttat ttcttctccc cgaccgggta ttcctcgaag atccgcacct
     5461 tgtgtgggtt gagatccggc cgcctgtctg tcatgaaggc ggagtatggg gtcacgtgga
     5521 tccggtattc caggtacccc tggatgaaat cggagaggcc ccggttccgg tggccgagga
     5581 tcaggatgac gaaccactgg atgatcaggc atgcggtggc gatgatgccg tacaccatga
     5641 gcacgaggcc gatgatgatc cagtagagga tccggacgag aagttcgagg cgcctcgcag
     5701 gctgctcgaa cacgaagagc tcggtaagat tcgacgggct tgccgttgtc ggctgaatca
     5761 cgtcttctgg catacctaag aaaaaaacag ggggagggga taatgatatc tcccgggatt
     5821 tgtcggggga agggtggctg cacggatgag aacccgaggg gagaaatccc tccccggtta
     5881 ccctccaggt agcgtctcac cggttcggta tttcccgagc tcctcctcca gccggcggtg
     5941 ctcggtgatg tctatgcccg tcccgatgac atgggtggtg gaccccttct cgtcgcgaag
     6001 aacggtgttg gaccaccgga tgtacttcct ctccccggtc cgggtcaccc agaagttgct
     6061 gagccggagc tgtccctgcc ctgcgacgag cccgtcgaaa ccctcccgca cccggtcccg
     6121 ttcctcgggg agaaggaaga tctcccagaa gggcctgccc ttcacctcct cctcggcgta
     6181 gcccgtgagg gcctcgcagg catggttgaa ccacatgatc ctcccgtccc tttccagaac
     6241 taccacgagg gcatcgagaa tgtgaagggt cgcgccgatg aagtcccgtt cgcggcggag
     6301 ctcctcgtcc agtcgccggt gctcggtgat gtcgctcccc accgagagaa ccccgaccgg
     6361 gttcccctgc tcgtccctga tggcctggtt ggaccaccgg acccagaccc gccggccttc
     6421 cttcgtgatg ttctcgttct cgttgctctc gtactcctcg gtgtgggaga ggagcctcgc
     6481 gatcatctgc tcgaggtccc gtcccgagct ctctgtcggg ggcacgatgg tcccgatgac
     6541 atttttcccg aggatctccg cccgggagaa gccgaagaac ccctcggcgt actcgttgaa
     6601 gaaggtgacc ccgccggcga ggtccatctt gagaatgatg gtgttcgcct tctcgacgag
     6661 ctcccggtac ttcttctccg actcccggag ggccttctcg gcgaggcgga ccggggtctc
     6721 gtccgcgagg atcaccgtgg atccctggct cccgtcctcg aagaggatgg ggatgacctt
     6781 tgcccggagg taacgctcgc ctccccggga gatctccaga ccggcatcgg tgctctcctt
     6841 ctcgagggaa gcgaggaggg cccggaccat gtcctggacc cccggttcgg gaacggggag
     6901 gtaggcgagg ttcttcccga ccacatcgtc ccgcccgagg gagaggaggg acaggaaggg
     6961 atcgttcacc tggagcaccc ggcggtcgcg gtcgaggatc atcaccatct ccgcggtatg
     7021 ggagaagagc tgcgggaggg gtacgcgctg ggtcagggtg aagaccttgg ccatgccgaa
     7081 gcttcgcatc tccacctggc ccgaggcatg gaggatatcc aggtacctcc ccaccgtgtt
     7141 cttggtcctc ccgagggccc gggcgagctc ggtgacggtc atcccccggg gctcgtcccg
     7201 gaggagcctc ttgatcacgc cgaagtcctc gagatccttg acagactcca aggtgtttct
     7261 cccgcagtca ggtgtatgat aattgtttat aatacttatg tatcatacat aaacgaaatc
     7321 cttatatgta caagcagact accgatcagg gacacatgat gatatccatg cacgcacacc
     7381 cggtaaaagg ccgggcggcc tcccggggag cgattccctg gatctgcctc gtcctcatcc
     7441 tcgcggcggt tccccttgcc gcgggcgcga gcaccacgga cccgaccgta gccatcaccg
     7501 gctatcacgt caccccctcc atcctcctcc ccggcgacca ggggaccatc gagatcacca
     7561 tcgcgaacac cgcatcgacg gcaagcacga cggaatccac cgtccagaat ggggtatcgg
     7621 ggtccacgac cacgacgacg acgaaggaca tgacggtcct cgtggaatcg gtctacatgt
     7681 acggccaggg ggtcgaggtg ctcgagggga acttccagca ggtcggagcc ctcgggcccg
     7741 gccagtccat gaccctcacc ttcctgatca aggcccctcc tcaggccggc atgtacttcc
     7801 ccgaggtctg gatccggatc cccgagggga cgagcgtgaa gtaccccatc ccggtcaatg
     7861 tcaactcccc catcggtatc cagaaacagg cggttctcgt cctctcgagc ttcacccctg
     7921 actcggtgaa cccgggggag gaggtcccgg tgaccctgtc ggtccggaat gacggggacc
     7981 tcctcgctga ggatgtcgcg gttcggatcg ggaacgtgag caccctcgtc gccccgaaag
     8041 gaaccgacct ctaccacctg ggcctcgtcg gctcgggccg gacgaagagc gcggaacttg
     8101 tcctcctctc cgaccgggac acgagcccgg gcctgaccca ggtcccggtg accctccagt
     8161 acagctccgt ggacggtgtc gtccacaccc aggcatcctc catcaacctg atgatgaagg
     8221 ggaagagcga gctcgggttc gtctcggtgg acacgagccc ccgaagggtc ggggagaacc
     8281 agccctttga cctcaccatc cggatcgaga acaccggtac cggcgaggcg aagcaggtgg
     8341 cggcgaccat cgacctgccc atgaccggga cgaagcagtc cttcatcggg aagatcaagc
     8401 cgggcaacga cgcgccggcg gtcttcctcc tggacggggc aaagggcggg agctatgcct
     8461 acaacgctac catcagctac gtggatgacc tggggtccca caccgaggtc aggcagatgt
     8521 ccgtccggat cgatccggcg gactcgagcg gcggcttcat cctcctgctc ctcgtcttca
     8581 tcggcggcgg actcctcgcg taccggttct ggtacatccc gaggaagaac ggcggcggtg
     8641 ccctcccatg ggtcagaaag agctgaaggt ctccctcttt ctggcctggc ggtcgctgat
     8701 ccgggggaac cggtcgagca cggtcatgac cgtgatcatc atcgccctct gcttcacgaa
     8761 catgatcttc ctccccggtc tcttcaacgg gatcgggcag agtatcaccg cacagatcgt
     8821 ggactacgag gtgggaaacg tcctggtcag cccccgggcg ggcgaccagt acatcacaga
     8881 cctccatgcc actctttccc tgatcaacag tctgcccggc gtggaacggg cgactcccca
     8941 ctacaccaag ggggcgaccc tgaaatcaag agaccggatc ctcggcgtcg gggtgcgagc
     9001 catctcgccg tcggacgaaa agtacgtctc ccccctctat acgaagatga tcgccgggac
     9061 ctaccttggc gacggggaca ccggggagat tatcatgggg aagacggtcg ccggggatgc
     9121 ctccgtccgc caggaggacg agttccagcc ttcgctcggg ggagtgcggg tcggggactc
     9181 gattaccatc gagtacggta acggctacac gaaggactac cgggtcaagg ggatcttctt
     9241 caccggctgg agccaggcgg acagcacggt gtataccacc atgagcgaca tggagctcgt
     9301 ggaagggaag gccctcgacc aggccgatta catcacggtg aaggcgatgc ccggttactc
     9361 cgagaagtcc atcaagaacg agctccagtc ctatggtgtc tcgcaggacg tccagaccac
     9421 gagcgacctc ctcgcgaagg ggatggggag ggtgctccag agctttgcca tcatcaacct
     9481 ggtttcgctg atcgtgagca tcatcatcac caccgtggtg ctcttcatcg tcatcaccat
     9541 caagaccatc aacagccgga agcagatcgg gatcctcaag gccatcgggg tggacaagac
     9601 ggtcatcatg catagctacg gcttccaggt catcatcctg gccctcttcg ggatcatcat
     9661 cggggtcatc ataacctcgt tgctcgtggt gtacatgtcc atccacccca tcgtgacccc
     9721 ggagtggtcg gcatacctgt tcctgacccc cctcgacatg ctgacgaact ccctgatcct
     9781 gttcggggct gcggtggtag ccggttatgt cccggcatgg cgggtcgcac tggaggacat
     9841 ccagaagaca atgagggcgt gatcccatga tccggacaga gaacctgagg aaggtctatc
     9901 ggatggggaa cgtcgaggtg aaggctctcg acggagtcga tatccacatc gagaaagggg
     9961 agttcgtagg gatcataggc ccctcgggga gcgggaagtc caccctcctc cacatgctcg
    10021 ggctccttga catccccacc gcggggagca tcttcatcga tgggacggac gtggggaccc
    10081 tcacggatta ccagaagacc atgttccggc tctataagct cgggtacgtc ttccaggact
    10141 acgccctcgt ccccgagctc acggtgaggg agaatgtggc gctgccggcg atgctgcggg
    10201 cggaccggag cgaggcgcag tacaaggccg acacggtgga catcctcacg aaggtcggcc
    10261 tgtgcgaccg gcgcacccac ctccagagcg agctctcggg cgggcagcag cagcgggtcg
    10321 ccatcgcccg ggccatcgtg aacaagcccg acatcctctt cgccgatgag ccatgcgcga
    10381 acctggacac tgagaactcc cggatggtcc tcaacctctt ccatgagatc aacaggtcga
    10441 tgaagcagac catcgtcatg gtctcccacg aggagtggca caaggaatac ttccaccgca
    10501 ttatccgtct ccgggacggg aagatcgagg acgacgggaa gtaagggaat ctccttttga
    10561 tcaaataccg gggctcaaga aatttcctat gccgggtgcc ggaaccggaa ttcctgggga
    10621 atcccggacc atttccccca cttatttaag ccatcgaaga tattctcctt cagggtagcg
    10681 ggcaggaagc gggggagagg gcatgatctc ggtactccac gtcgatgacg agcaggattt
    10741 cctggacctt accagggcgt ttctcgagaa gagcggggac atccggctcg aaggagtcac
    10801 atcggccgaa tcagccctgg agaggctcga ggaaacccgg tacgatgcca tcatctcgga
    10861 tttccggatg cccgggatgg acgggatcgg cctcctcaag catctgaggg agagcgggaa
    10921 ccgtatcccc ttcatcatcc tcacgggaag gggaggggaa caggttgcca tcgaggcatt
    10981 gaacaacgga gccgacttct acctccagaa gggaaccgat ccgagggtcc tcttcatcga
    11041 gctccggaac ctgatcggcc aggcggttac ccgccggcag gcagttgagg ccctcaaaaa
    11101 gagcgaggag tggtaccgga acctcaccga ggcgtcccag gatctcatct acatcatcga
    11161 tcgcgacgac aatgtcgtct acttaaacag gcattcccag gagctcctcg ggaaaaagac
    11221 cgaggaggtg gtagggaggc ccaggtcact cctcttcccc ccggatgcag atgagcacat
    11281 gaagagaaat ctcgcgacgg tcttctcaga ttcgcatccc atccgggttg agagtcttat
    11341 ccccgtcggc actcaggaga tctggctgga taccacgctc atccccctcc ggagcgccga
    11401 ggagagggtc acgtgggcca tggggatctc ccgggacatc accgaacgaa aacggatcga
    11461 ggatgccctc cgggagagcg aggagaagta caggaacctc gtggatcggg cctacgaggg
    11521 gatcatcgtg gtccaggacg atgtcgtgaa gctctgtaac cggaggaccg cggatatgtg
    11581 ggggggcgaa tgccgggaca tcacggggcg tccctaccgg gatttcatcg acccgagcga
    11641 gctcccgcgc ctccggagga actacgagct ccggatggca gggaaaaagg tccccgggcg
    11701 atacgatacg cggctcctgc ggagggacgg gacgagtttc cccgtggacc tggaaggggg
    11761 ggacatagtc ttcgacggca ggccggcgga tcttatcatg ttccgggata tcacggaacg
    11821 aaagaaggcg gaggtggccc tccgggagag cgaggagaag taccgtgccc tggtggagcg
    11881 ggcgaacgac gggatcatcg tggtccagga cgggatcgtg aagttctgca accggagggc
    11941 ggcggagctg tggggaggcg atgtcgggga gatcgagggg cgcccgctcc aggacttcat
    12001 tgacccggtg gacgtttccc ggatccggga gaattacgag cggcgcatgg ccggggagga
    12061 ggtcccccgc cagtacgacg ccctcctcct ccggaaggac gggagccggt tccacggtga
    12121 gctgaacgca ggggccttca ccttcgaggg aagggctgcg gatctcgtcc tgttccggga
    12181 catgaccgaa cggaagaagg ccgaagaggc cctgaaaaat gtcaaccgga agctgaacct
    12241 cctctcctcg gtcacccggc acgacatgct caaccagctc gtggccgtcg tcgggtacct
    12301 ggaactcgcc ctggagagcc cctctcccgg agatgcgagg gaactcgccg ggatggcgag
    12361 gaaggccgcc gtgacgatgc ggcaccagat cgagttcacc cgccagtacc aggaggtggg
    12421 agtccattcc ccggtctggc aggatctccg cgaggtcatc gcacgagcgg cctccgatgc
    12481 gggcaacggg agggtgcggg tggagatcga cctggatccg gtcaggatct atgcggaccc
    12541 cctcctggaa cgggtctttt ccaacctcat cgagaatgcc atcctccatg gccagaaggt
    12601 cacgaggatc aggttctcgg tccgggcaac cgaccccggt ctcgccatcc tctgcgagga
    12661 tgacgggatc gggatacccg cccatgacaa ggagcggatc ttcgcccacg ggtatggaag
    12721 aaagaccggg tacggcctct tcctggtccg ggagatcctt gccatcaccg gggcgaccgt
    12781 ccgggagatc gggcagccgg gagagggggc cgtcttcgag atcaccgtcc ccagggatgc
    12841 gtacagggcc ggagggtgaa ccggcatggc tttccggtgc aggatgtgcg gaacatgctg
    12901 catgtccatg ggcgactgca tcgctgtcga acgtgagatc ggtcccttcg agttcgagtg
    12961 cgaatcgatc tcgacgggga cccccttccg tgccatcgtc gacgaggata aaagggagct
    13021 gttccgggac atcacctgga tcaaggagca ccctcgcgcc tgccagtttc tccggccgaa
    13081 gggagaacaa gttctctgca ccattcacgg gacgagcccg gcccagtgca agtattaccg
    13141 gtgcgtggtg atgcggatct tcgaacccgg tggggatctc gcaggcaggg tgacaggcac
    13201 cctcgccctc cattccgata atccggtcct gcgggagatc tgggaggccg ctcttcggga
    13261 gataccggaa ggggccggcg atgtggaact acgcctacag aaggtcctgg agagccatgg
    13321 ctgccgggtc gaataaagat ccaaagaagg tcacgaaaac agaagaaaga aaataaaaat
    13381 tatgagatga tgagattaga accgggggcg cctgtgcttc ggcaggcact cctggcagta
    13441 cacgggccta ccctcggtag gcttgaaggg gacttcgcat tcctttccac agtctgaaca
    13501 gatgaccttg gtcatctcac gcgggccgaa gtttctgcgg ccaccaaaat ctgatcgata
    13561 catggcttta cctaaacagt cttatgaaaa actgcattat agtattgact ctccgtatgg
    13621 atgaagcttt gtatccggga gatatattca gatgtaccgg ttccgacgga gccactccac
    13681 ctggggcggc aggtaccggt acaggatatc gcacttctcg tcctggaagg accagggggt
    13741 gacgatcagg atgtccccct cccggatcca gactctcttc ttgatcttcc ccttgatcct
    13801 ccccatccgg gtgaccccgt cgtaacaccg cacccggaca tggttggccc ccatcatcag
    13861 ctccgccgag gcaaactgct cccggttcca tttcttcgga agccggaccc ggacaatggg
    13921 ctggccctgg gcatctaccg cgtttggatc aactgcgtca ttgtttttct ttggatttcc
    13981 cggaattcaa tcagctccgt tggcataaat gaaggtcgtc tgatgcaatg aaggtatgcc
    14041 tccgtcacgc ccctccctgg gcgagcatcc cgatccggga tgcgtaaagg tgggtgatat
    14101 acccgaggag ggccgctgca gccgggacga ggaggagggc cccgtagata cccggtccgg
    14161 gatccccgat ggacgcccgg acgatcaggt tgagggggtt ccccgggatc gcaaccacga
    14221 ggaggatcac gatcacgagg gccgtcgagt agatgaactg ggcggcggtc cgctcccggt
    14281 agtggagagc ggtgagggcc gagaggagga tcaggatgag ggatgcggcg gagacgagga
    14341 ggaggatggg aaccgggttc gagatccgga tcccgttcac cgcgaggagg aggagccagg
    14401 cggccgcctg gacggggacg aggatctcgc aggcgaggat cttgccccag accatctcgg
    14461 cgaaggtgac cggggtggag atgagggtct cgagggtctc gtgctggtac tcctcggtga
    14521 tgaggtcgat gacgagggag gcggagatga tggcgggcat gaacatgagg agggggatga
    14581 ggaggccgta cacgaactcg tagaacccct cggccggggc cgccggggga aacgcgagtt
    14641 cgatgggctg cgccgagatt ctcgccgccc gggccgtccg gaggtctccc tcgaaggcga
    14701 ggaggacctc cttcagcttc acccccacca ccgtggcctg caggtcgttc tgcagcgtgt
    14761 agagggtgac ctggacggga tccggggagt cgggagggat gtcgggtacg aagaggaccg
    14821 ccgcaagctt ccgctccttc agggactcga tggcgggaga gaggtccatc tcgaacacgg
    14881 tgaaatcatc ggagtcctgg agccgtccga ggagggtcga atttgccccg gtgtaggcga
    14941 tgctgtaccg gaacctcgag tactgggaga cggaggaggg gtcatagagg gaggtgaggc
    15001 cgaccatgag gaaggaggag aacatggcca cgaagagctg gaggaggatg gcgaagagga
    15061 tggtcttctc gtagttgatc cccgagagct ccttcctcgc gacgatccag atgtgccttc
    15121 ccttcacccg agcacccccg aaaggaggga gaggttatag aggctgtgta cgaccgccgc
    15181 ggcaaggatg ccggggaggt accagcgccg tccgcctgtc cggaggacga ggatcacgat
    15241 gagcaccccg gtgaagtgga ggaggaaggg catccagagg aggaggatcc cctgctggag
    15301 gaagaggacg gacccgaaga ccgagtcggt gatctgggtg agcatggcga ggaggaagag
    15361 cttctccccg aggaggaacc ccgcggcggt gaccgccgag gcgaggatca ccgtcctcca
    15421 ggggaggccg gacagctcgg cgtggaagag gctgtacacc ccgacggact tggcgatctc
    15481 ctcgatgagg gccgcgagga ggatgaggag gacgagggag aggggcatgg ggaggttgaa
    15541 gaggaggacg aggcccatca tctgggccat aaagacgaag gggatgagga ggatgttcgc
    15601 gacgaggagg gaccgccagg ggtgttcccg ggacaggctc gaggtgacga actcccggag
    15661 ccggggaagg aagggccgga ggtcgaagag gcgctcggca ttgaagttcg cgaccccgat
    15721 gaagaggagc accccgcttg tcaggtagaa gagggaggtg gaatacaggt agtccgtggc
    15781 cgtaaagccc gtgccctgga tctggaggat gatgagggtc aggggggaga cgagggagat
    15841 gatatggaca ttcgcgaaga tggaggggaa gaagaggtag gaggtggcga gggtcgagaa
    15901 gaagatggat atgaaggaga gctccttgaa ggagcgggag agcatcccga tgaggagggc
    15961 cgaggcgagg aagaagagga tgaccgggag gagggggagg aggatgagga gggatgcccg
    16021 ggccgtgagg atcaccaccg aggaggtaaa aatcatccgc gcgaggtagg ggagcatctt
    16081 ccccgcgatg atgacgacgg gggagagggg gcccgagagg aggatctccc ccttcctctc
    16141 gatccgctcg ttcatgacgg acatcatgaa gaactgcgac gtgaagtaca gggggaagat
    16201 gaagacgaag atgaagatga tggagtcaaa ggggagggag ggggagagct gggagggggt
    16261 cctcacactc gagttgggag agggaagacc ggtgaacatg cccgtgtacc gggcgacctg
    16321 atcgtttttc ccggcctcct gctggaggtt cctccggagg tcgtcggcgg aaacggggag
    16381 ggttgcggcg gggggcgtga cagggacgac cggtccctcg ggtacggggg gagcggatga
    16441 cctctgccgg acggagatgt actgtccctg ctgggtggcc tggaaatcga gctcgctcct
    16501 cacgtactgg agatcgatcc agaggggata ggcggcaaag aggtcaggct cccggttgta
    16561 gaccgaggca acgtactggg agtagtcccg gcccagggtc ttcagggcag aggccccctt
    16621 ttccgagagt ggtgggagga ccgtccggtt caggacgacc aggtcaaagg ccgcccgctg
    16681 agaggggagg gtagccccat ctccgatgaa gaccgtgaac ctggcgtcgg cggcgaggat
    16741 cgccccgagt tcacggtcat cggtggcgag ggtgtacatc ccgtcctgga ggtgcatccc
    16801 gctccgggcg acgaacccgg tggagaggat gaggaggatg aagaggacga cggcgagggg
    16861 aaggacctgt ctccccatgg tcgagaagga tttttttacc tcccaccgcg cgatcgtcag
    16921 aatatccgaa aatattccca tatttatcta atataccttt aaatatcgcg tatatcagaa
    16981 cataaatttt atcaatttaa tggataaatt gtaataagtc atgatctctg cctgcggact
    17041 ctccaagacc tacgatggct ttccggccct cgacggagtc ggcctcgccc tcggggacaa
    17101 cgagatcctc ggtgtcatcg gccacaacgg ggcggggaag acgaccctcc tcaagatcat
    17161 gacgggcctc gtgcccccga ccggcggaga tctcacgata aacggcatcg acgtggtgga
    17221 ggaccccatc gggctgaaga agatcctcgg ctacctcccc gaggactccc acctctacga
    17281 gacgatgacg gtcaggtcct acctcacctt cttcggggag atctacggga tgggcaggga
    17341 ggcgatccgg gagagggccg accggctcct cgccaccctc tccctcgagg acgggggaaa
    17401 gaagatcggg gagctctcca aggggatgaa gcgcaaggtg gccatcgccc ggtccctcct
    17461 ccatgacccc gccgtcctta tctacgacga gccgtcctcg ggcctcgacc ccatgacctc
    17521 ccgcaccatc atcgagttcc tgaaggagct ccggggccag gggaagacca tcgtcttctc
    17581 ggcccacaac ctgtaccagg tggaggaggt ctgcgaccgg gtgatgatcc tccggcgggg
    17641 gaaggaggtc gcctacgggt ccatccccga gctccgggag atgttcgggt ccatcaccta
    17701 caccctcacc ttctccatcg cggatctctc ccggctcggg aaccaccgta cctaccggaa
    17761 ggacggggtg tactacgcgg tcgacgggct cgacatcgag gggatgaacg agctcgcgac
    17821 atcgctcccg gcctcggggg gcagggtcga gcgggtcgag tcccactacc cgaccctcga
    17881 gcagatgctc gtgaagatcg ggaactgaag acttcgatag tatcgatatc gttgagtaac
    17941 cgaacctatc ggttctcgtg ggtctgcttc ttcgtaatgg gctctccgac ctagagcgac
    18001 gaagtcacga agggctgcgc cacccctggc gcggccaacc ccccagtgta ccgggtattt
    18061 ggggacggtt tttaaaaggt ggaatggctc acgagggatg agcccggatg gtcagaccaa
    18121 tccctgagaa gcccccgtac atattgatgt gataggtccc cggggatgag aaaaataatc
    18181 gtttcgggct gtttgtactg taaggaccgt aaaaaccttc ctgggacagg agttgttggg
    18241 tctgattatc tctgatggtt atgtacacga aggtccgtgt aggacttata aaccgggtga
    18301 gggcgtagtc taggatgaga ggtggtctgg ggacatttac caccaccgct tcgtcttgga
    18361 aatctagatt gtaagattgc tggaagatta ccgggggcag cccggtggct ctcggatcat
    18421 acacggtctg gtgatagggg agaaggtagt atttcttgtc atggaaggga gtcgctggtt
    18481 ggacccaggc cctggacatg tccttggtcg gagggagact ggggatttca atggttggct
    18541 caggggtcgg cgggatggtc tcggcgaggg tggtttcaac gggagtcggg atctccgtga
    18601 ccccggtgtc cgccggggcc ggcgtcggga caggctccgg ctgggtggtc ctgagataca
    18661 ctcccccgag gaggatgatc atggcgacga ccacaaccac cgcaaaggca accgctacgg
    18721 gttccatcaa ccacttcatt ggtaatcacg gcccagaagg ctctacatat gggtaggagc
    18781 cacaaggata agccattttc ccagtaaaat catcccccgc cggcattgca gaatggaaat
    18841 gcttaaattg atctttggga aagaaccacg accccccgga agacacgcga ataacgtact
    18901 cgaggctttc gagaaagagt gcggaaaacg ggcctgcgtg aggaaaatgc ggaaaacggg
    18961 cctgaatgga ggcgaatgcg gaaaacgggc ctgaatgagg agattgcgga aaacgggcct
    19021 gaagggattt gaacccctgg ccgatggatt aagagtccat cgctcaacct gactaagcta
    19081 caggcccagc agacgtatta tgtggtcaac ggattcccat aaggatttcg ggtactgggg
    19141 gcaggagtcc cgcagggttt tcggaatcag gggttcggag tctcgcaggg cgacgaagcc
    19201 ctgatgagaa aatcgcgaag cgattttcgg aatcgggatt gcggaagccc gaagggattc
    19261 cagaatcacg aagagagatc acctagtgat ctcgcaatcc cttatatcgg tggagggcgc
    19321 aactctgtac atatggccgg cagggatgcg gaaaagggga aaaagaaggt caaatacctg
    19381 atattcgggt gcgggagcac cggctacaac gtggtggagg cgctcctccg ggagaccccg
    19441 gacatcctca tcatcgaccg ggacgagaaa cgggtcgagg acctgcgggt ccagaagttc
    19501 aatgccctcg tccgtgacct gacgagcccg gccctcgtca acggcctccc cgagcccgac
    19561 gtcgccctcg tgctctcgaa cgacaaggac gcgaacctcg ccgccgtccg caccatccgg
    19621 tccgcatacc cgaaggccca catcatcgcg agggcgaccg accctgtctc catcgggatg
    19681 ctccaggagg cgggagggga cctcgtgctc tacccccagg aggtagtagc ccgggaggtc
    19741 gagggccacc tgctgcagct ccactcctcc cggctcgccc gccggctctt cgagctcatc
    19801 gccggctggg aggggaccct cgggattgtc acccacacga acccggaccc cgattccatc
    19861 tcgagcgcca tggccctcgt ggccatcgcc gctgaggcga acccgaagaa cctcacctgc
    19921 cggatcctct acggggggaa catcggccac caggagaacc gggccttcgt gaacctcctc
    19981 gacatcaaca tggagcggat caccccccag gtcctcaagg agtgcgacta cctggccctg
    20041 gtcgactccc tcgggcccgg gatgaacaac gacctcacga agaagaccca ggtgaatatc
    20101 gtcatcgacc atcacacgaa cggggagatc cccgaggtga agggcgactt cgtggacatc
    20161 cggacgggca tcggggcgac ggcctccatg atggcccagt acctgaagga gctcgggatc
    20221 gtcgtgaaca agaaggtggc gaccgccctc ctctacggta tccaggctga cacccggtac
    20281 ttccgccgga acgtcacccc cgccgacttc gtctacgccg gcttcctcct ccccttcacc
    20341 gacctcgagc tcctggagaa gatcacctcc ccggccgttt cccaggagac cctggatgtc
    20401 ctcggggccg ccatcaggaa ccggaagatc cggagcgggt acctcttctc gaacgtcggg
    20461 tacgtgagga accgggatgc cctccctcag gcggccgacc tcctcatcaa cctcgaaggg
    20521 gtgaacaccg ccctcgtctt tggcatcggg gactcggcca tctacatctc ggcccggaac
    20581 aaggacatca ggctccacat cgggaacgtc cttgccgagg ccttcgggga catgggggag
    20641 gcgggcggcc acccgaacat ggcggcggcg aacatccccc tcacctactt ctccctcgtg
    20701 aagaccaagg acgacctcct caccctcgtg ctcgaaccca tcatgcgcaa gttcatgcgg
    20761 gtcgtgggcc tcgagaagga agagtcccat gagattttcg agatttagag gcgtacacgc
    20821 cctaaatctc gatatttcag ggagtacgcg acatggaatt ttcgagattt aatggtatac
    20881 gtccatgaaa tctcggaaaa atgggctcat ttttgcaggg tttgaggact gggagccgta
    20941 ttacctccgg atcctcgaac acttccactt tgaccgggcg cgagacgagg aggcggccct
    21001 cctcctcgcg agcctcctcg cgagggacga tctccccgtc gccgatgcgc tcatccgggg
    21061 gaaaacggcg accgtctgcg ggaacggacc gaatctcacg gacgagctgg accggatccg
    21121 ggggaccgtc atcgccgcgg acggggccgc gaaccggctc ttcgcccggg ggatcccgcc
    21181 cggggtcatc gtgacggatc tcgacggtgc cacggatgcc ttcatcagga tgaacgtggc
    21241 agggacggtc atcgtggtcc atgcccatgg cgacaacatg cccctcctcc ggagctgggt
    21301 gccccggttc cccggccccc tcgtggggac cacccagagc cggcccttcg gccggatcca
    21361 caacttcggg ggattctcgg acggcgatcg ggcggtcttc cttgcccacg ccctcggggc
    21421 ctcggaggtc gccatcgcgg gcttcaacct agacgatccg aacgtggatc cggtgaaacg
    21481 ggaaaagctc ctcttcgccc gggagctcct cgggctcgcg ggtcatgacc tctgacgcgg
    21541 tcatcctgga cgggtacatc gacgagcccg cctgcctcgg ggtcccgccg tacatcgccc
    21601 cccagccccg ttccctcgcc ggagtcctcc gggaacacgg gtaccgggtc aggtatcaca
    21661 ccatcgacca gctgaggcag gacccctctc ttctcctctc ccttgatacc gcggatatcg
    21721 cggtcatggt ggccggggtg acggtccccg ggaagtacct cgccggcacc cctgccacgc
    21781 tcaccgaggt cgagcaggtg ggagccaccc tcaggcaccc gaagaccttc ctcgccgggc
    21841 ctctcctctt cggctattcg ccgcgggggg gagaacgggc gaaaagaagg gagatcgccg
    21901 gattcgcggt catcctggag gggtcaccgg cgggtgccct ggattccttc ctccggggcg
    21961 gcgaaccgag gagcgacggt gactaccggc agatggccgg atggatggtg gccggggctc
    22021 ccgtgatcag gcagcatccc tcgttcccgc acctcatgtg cgagcttgaa acggcgacgg
    22081 gctgcagccg ggcggtctcc ggaggatgct ccttctgcac cgagcccttc tacggtcccc
    22141 cccggttccg cacggccggt gagatagtgg ccgaggtgtc cgccctgcac gcgaacggtg
    22201 ccttacactt ccggctggga aggcagcccg atctcctcgc ctacggggtc agcgggggag
    22261 agttcccgcg cccggatccg gagaagatcc gggacctctt caccgggatc cggcaggcgg
    22321 ccccggatct cctcaccctc cacatcgaca acgtgaaccc gggaaccatc gcccggcacg
    22381 aggaggcgag ccgagaagcc ctccgggcca ttatcggggg acacaccccg ggggatgtgg
    22441 cggcctttgg catggagacc gccgatccgg ccgtgatcgc cgcgaacaac ctgaaggcga
    22501 ccccggagca ggtgacgcag gccatccggg tggtgaacga ggagggggga aggcgggagg
    22561 aagggatccc cgggctcctc ccggggctga acttcgtcat cgggctcgcc ggtgagacgg
    22621 aggagacctt ccggctgaac cgcgagttcc tctcgcgggt ccttgcggag gggctcctcg
    22681 tccgacgggt gaacatccgg aggctcatgg ccttccccgg cacccgggcc ttcgacgtga
    22741 acaccctggg gaaacacgac cggttcttcc gcgccttcaa ggagggggtg aggcgggact
    22801 tcgatcatcc gatgatctcc cgggtcttcc ccctcgggac catcctccgg cgggtcgtgg
    22861 tcgaggtgga gggccggatc tcgtacggcc ggcagatggg atcctacccg gtcctcgtcg
    22921 ggattcccct cccggtgcag gtgagaaccc tgctcgacac ggtggtcgtg gactggggga
    22981 tgcgatccat caccgccctc cccttcccta tcgagataaa ccggctcccg gtccgggcgc
    23041 tctcctggat cccggggatc gggaagaaga aggcggcagc cctcgctgcc acccggcccg
    23101 tcagggacct tgcctctttc cggtcagtcg ccggggaaac cccgctggaa ggcgccctct
    23161 ccttcgttcc ctaggcctcg cggagctcca tcaccttgag gatatcggtc cgggtgacca
    23221 ttccgaggag ccggccgttc tcgatgatgg ggatacgccc gacgttgtac cgggacatga
    23281 gccggaaggc ctcggagagc atggccgtcg ggggaacggt gatgaggttc ctcgccatga
    23341 tgtccttcac ctgctttgcg tcccggtcga tctccgagac ccggtggacg tcctccaggg
    23401 tgaccatccc gacgggcact ccgttttcga ggacggggaa cccgaggtgc ttcgtcgcgt
    23461 acatgagccg gaccacctcg ggaaccggga gctggggctc gacggtgacc aggttcgtgg
    23521 acatgacgtc cttcactgcc acgtcccgga ggaggaccgt ggaccggacg gcgtagaact
    23581 cctggtctgc cccgaggtag atgaagatcc cgatgatgat gaggatgaag ttcagggtga
    23641 ataacccgaa gacgacgaag atgagggcaa aggccttccc gatgtcggcg gcgatcttcg
    23701 tcgcccgggg gaggggcatc cgcctcgcga gccatgcccg gagcaccctg cccccgtcca
    23761 tgggaaaggc cgggaggagg ttgaaggcga agaggatgat gttgaggagg gcgaggtacc
    23821 cgaagaggaa gatcacgaca cccttgaccg ggaggtcact gatgaagggc gctccggcga
    23881 tgaccacgat ccaggagagg atgccgaggc cggcgctcgt cacggggccg gcgagggaca
    23941 tggggagctc atcccggggg tcggggagct cgttctcgat ggaggagacg cccccgagga
    24001 tgaggagggt gatgctgttg atcttgatcc cccgccggag ggcgaccacg cagtgggcga
    24061 gctcgtgcac gaggacgcag aagaacaggg cgagggcgac gatggcgccg aggatgtagg
    24121 aaatccaccc ctgggtgatg aggctcgtgt tcgccccggg caccccgaag atgccaaaga
    24181 cgaagctcgc cgatatcgcg atgtccgtcc cgatgatcca ggcaaaaagc gggatggcga
    24241 ggagaaagga ccagtgaagg aggaccggaa taccaaagag gctcccgatt ttgatggaac
    24301 tggccatggc agacgagtat ctttttaatg tttaagaaac tatcctcctc tgataaggat
    24361 gattacgcag ataaccgaac tgttcgggct ctcggtgtac acggataagg caatctacgt
    24421 gggggacgtc gaggatgtgg ttatcgatgt ggacaacaag aagctcgatg ccctggccat
    24481 cgggaacctg aaccccgagc tcgtggacgt gaaggggttc aagggcatca agatcccctt
    24541 ccggatcatc aaggccatcg gcgatatcat cgtcatccgc cacctctccc cgtccttcag
    24601 gcaggagtag gaatacccga gaggagagcc cggtcccacg ggagcgggat agccccgtct
    24661 cccccgctgt ttttccatgc aggaaagaga gatcttcggg aacctcgcca tcgtcccccc
    24721 catcttcgtg aggctggacg ggagagcctt tcaccggctg gcaaaggacc tctcgctgga
    24781 gcggcccttc gacgagggtt tctcccgggc gatggcaggg gtctcctccc ggctcctcac
    24841 cgagagcggc ctctcgcccc tcttcgcctt taccttctcc gacgagatca gcctctactt
    24901 cccggccctc cccttccggg gccgggtcga gaagatcgac tcggtcctcg cttccttcgc
    24961 ggcgagctgc ctcaccctcg ccctctcccc gggccatccc ctcgccttcg atgcccgggt
    25021 catcatcgca tcgcccggct atgcccggga gtacctcgcc gggcgccagc gggaggcctg
    25081 gcggaaccac ctgaacgcgt actgccagga ggccctgacg atggaaggga tgaaacccac
    25141 ggaggtggcc cgccggctct cgggcatgcg gggcgaggag ctccacgagt tcatgttttc
    25201 ccggggggtg aacctggcaa agaccccggc atggcagagg cggggggtcc tcgtgtaccg
    25261 gagaggtgaa gaaagggccg gttacaatcc cgtgaagggg gaatccgtgg agacgatccg
    25321 gcgggttccc accgtggacc gggacctccc cgtgttcacg agcccggagg gccaggccta
    25381 cctcgacggg atcctgggcc cgtcaggccc gtgaaacggt cgtccggacc ccgactccct
    25441 cgatctccca gtctgcctcg aggccgcctt ccggcgaggg gcctccttcg ccgggcaggc
    25501 ggatggagag ggaccgcgcc ctcacctcgt cccggatccg gttttcccat ccggaccgga
    25561 cgagggaggc gaccctcgga tcgtggatga ccactcctgc gacgatgaaa tcctcgaccg
    25621 cgaggtcgag ctgcctccgc atctcctgga tccgccggat gatttcgcgg gcatacccct
    25681 ccccctcgag ctcgggggtc agccggccgt ccacgtacac cacggccccc tccatgggtg
    25741 ccgaggtgaa accatcgggg atgacccgtg cgaaactgac gtgtgcggga gttacctcga
    25801 aggatctccc cccgccggtg agggggaact tcccgtcgcg ttcgagtccc tcccggaggg
    25861 atcgggcatc ggcaccctcg atgagggcct tcaccctcgg ggcatccttc ccgaaggccg
    25921 ggccgacggt acgcatgacg ggctctgccc ggatccccat ccggtcccag gatccctcga
    25981 cgagggtcag cctttttgag ttcgcccggg tccgggcgat ctccccgaga tcccggaggg
    26041 ccccggcaac cgggggcgat gcggtctcca ccaccacctc ggcgacgggc cagcggagct
    26101 ttcgtttcgc ggcctgccgg gcattggcca cggcgtcgtc gaaggcccgg accacggcca
    26161 tcccggtctc gagatcccgg tcggcgagct cagggtctac cgatggccag tcgagcatgt
    26221 gcacgctcac gggctctccc gaaagccgaa ggttcccgta gatctcctcg gcgaggtggg
    26281 gagcgaaggg cgagaggagc tggacgagcc ggcgcataac gaggtagatg gtgtcgtacg
    26341 cccgccgctt cgacggggag tcctcctcga gccacatccg ggggcgcacg agctggacgt
    26401 accaccggga gaggtccccg aggatgaagt cgaggatcgc cctcgtcgcc cggtgaagct
    26461 cgcagacctc catggacgat tccacctcgc ccgaaagggt cgcgacccgg gagaggatcc
    26521 accggtcctc ggggggcatg gcggggattc cgttaaggat ccggtcggga tcgaaccgtc
    26581 cctcccgatc ctctggcggt ttgtacccgt ccaggatcat gtaggggagg ggaaaccggt
    26641 agacgttcca gaggatgttc accgcccggt tcacggtctt caccccatcc cagttgaact
    26701 tcaggtcgtc ccacggggcg ttcgccgaga gaatatagag ccgcagcacg tccaccccca
    26761 ccttctcgat gacctcctga ggggcgacga cgttccccag gctctttgac atcttccggc
    26821 cctcggcgtc cagggcaaat ccgtgcatga gcacgctccg gtacggcgac cgggagaagg
    26881 cgatggtgga tgcgccgagc tgggagtaga accagcccct cgtctggtcc t
//
LOCUS       DATGDI010000222        10326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_432080, whole genome shotgun sequence.
ACCESSION   DATGDI010000222 DATGDI010000000
VERSION     DATGDI010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 10326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10326
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432080"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..502
                     /locus_tag="VKO45_08385"
     CDS             281..502
                     /locus_tag="VKO45_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05927.1"
                     /translation="MALRRAEKLQSWYEFGGKAGTVIGIFIVALFFMENFLLGFIAMC
                     IAFYTTRFVAVKFFQRLEDFIWGPEADPA"
     gene            564..950
                     /locus_tag="VKO45_08390"
     CDS             564..950
                     /locus_tag="VKO45_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05928.1"
                     /translation="MIDPVELAELADRILAIAGENQKTLAEVLDTLDAEARRELLGSD
                     LLNALQAFYYHFREMPTLLGQERLTLQPASAALEGITFEEEKGVDLVFRVEESGPVVE
                     LSELGEIIAKFEGASAYRGARRYLED"
     gene            complement(889..1668)
                     /locus_tag="VKO45_08395"
     CDS             complement(889..1668)
                     /locus_tag="VKO45_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HMA05929.1"
                     /translation="MIREHFQFRETIATILADDPSHITAAKEGMMSARQELERYIARD
                     PFFAITYDPYTPEGDGKVVMRMAEAAGKAGVGPMAAVAGAIAWSGVEAMAGSGARFGV
                     IDNGGDIALVTDRELRIGLHAGSSPLSDRFAFLVPPGGGIRGVCTSSATVGPSVSLGV
                     ADSVTVFSENPASADAWATAICNKISPGNRDVLGRLGGTDITGVVVILGEEILPWGDP
                     LPLVPARVDPDIITRGKDPTRFNPPGNASPPGTRRPPRTSR"
     gene            1800..3908
                     /locus_tag="VKO45_08400"
     CDS             1800..3908
                     /locus_tag="VKO45_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMA05930.1"
                     /translation="MTGEKPNRLIHEKSPYLLQHAYNPVDWYPWSEEPFRRARAEDRP
                     VFLSIGYSTCHWCHVMARESFDDPEVAALLNASFVNIKVDREERPEIDQVYMEVALAI
                     MGTGGWPLTLVLTPDGKPFFAATYIPRKSGLGLVGMLDLVPGISSAWRERREELLDAA
                     DRVTVLLRSRPPSGDGGDAVAVVDQVLDDLKAGFDREHGGFGGAPKFPTPHALTFLLR
                     EFRKRADPELLAMAEKTLLSMHAGGIYDHVGSGFHRYATDAGWRIPHFEKMLYDQALL
                     AMAYTEAFLITGKAVYRKVAEETLGYMLRDLRSAEGAFFSAEDAESGGREGGYYLWTW
                     DGIGEVLTGSERAAFTVYYGICRDGNMPRHPGVTASGENVLFIADPGGEAPGETGLPR
                     DALTGTLSRAMEKVRAARNRRQRPSRDEKILTDWNGLAIGALAMASRAFGSAEHREAA
                     GETARFILTRLATKEGRLLHRYFGGEAGVEGNADDYAFLAWGLIELYETTFDTRFLEQ
                     AAVLTDVLLSRFWDREQGGFFFTPDDSEALIARLKPVHDGSVPSANSVALHNLILLFR
                     LTGRTRYLDRARELMAWYLRERSSTAPAATWMMASLSLAFSPSTEVVIAGDPDAPDTR
                     ALLEVAGTRQHPGVSVLLKRPAGDPLLDSLAPFTRDFTTRDGMAAAYVCRNHACELPV
                     TDPESLARILDAPPGPGSKG"
     gene            3980..6037
                     /locus_tag="VKO45_08405"
     CDS             3980..6037
                     /locus_tag="VKO45_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HMA05931.1"
                     /translation="MTGRLLTEPEGYGLLDRSGIPVPPHGVARSPDEAARIAEGIGFP
                     VVMKIVSPDIVHKSDTGGVVRGIHNAAEVRPAYQEILSRVKARVPDAAIHGIIIEKEL
                     PPGLEILIGGKTDPTFGKVITFGLGGTLVEILRDVAIRLLPLDLTDAQDMVREIRGYR
                     LIAGYRGEPPKDEAALVSLLLATAEMFAREDISEFDLNPVILYEKGACVVDARMYLGG
                     EAPAGPRKIHCALAPEIFSPESIAVVGASGDPSKVGYAVFRNLLPFPGRLYAVNPNRS
                     EVQGRGTYPSLGALPEPVHLAVIAVPARLVPGIVEEAGKAGAKAAIVLSGGFGETGAA
                     GKALELEVLEIARRYSLRILGPNCLGIMLPHKGVNTTFGPITPRPGRVAFISQSGAVI
                     ATMVDWSVPEEFGFSAVVSVGNQADLGFEDFLFYLGGDPETRAIVMYIEEVKDGPAFL
                     EAVERASARVPVIAIKTGLSAKGRAAASSHTGALAGSHEVYMAAFRKAGITATQSLRE
                     AFLLAELLASEGYPAGRRGIVITNAGGFAVFASDYADLYGVDLVDLHPATLTELNGFL
                     PASWSHGNPLDLVGDSGVDLYARVFDFLIKHQEFWDIAFIVSVPDAVLDPTQLAQEVV
                     RFSRNTKKMVVGCFLGGSSVTASIQILRGHHIPNFSDLEDAFRVVGRACGLKKQGNPR
                     NPG"
     gene            6106..6684
                     /locus_tag="VKO45_08410"
     CDS             6106..6684
                     /locus_tag="VKO45_08410"
                     /inference="COORDINATES: protein motif:HMM:NF039541.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5804 family protein"
                     /protein_id="HMA05932.1"
                     /translation="MKLLLIERDGTDLYSTLYDSDTSRAMLRFYHPVRHPAGVEIQTA
                     SLGSALSLTSELRWYIQRYVDEVLFTEDGTVYLTWSLADEVYRRGAILSLPWPHRFRY
                     RIHGGKVYRSPWDLAPFGAPPPEPSAEEAALTGVEDRDLILEVWSTAEEYAEPERGEP
                     IGPAGPPVTEEAGEQGEPPEPESPGEPGPVPL"
     gene            6764..7438
                     /locus_tag="VKO45_08415"
     CDS             6764..7438
                     /locus_tag="VKO45_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05933.1"
                     /translation="MARKILTIGITINLENYENLRLEVQGEIGSDGNTEELISFLDGM
                     LARLGHGDQVTAERVEAYRRRVLAMPGSAVIAAVPDWKADGTEVPGPRPAPVVIQAER
                     VITPREPQPPEIPQEAPPPGEAPAAPATVRVVSGGRGAKPISQAPARPEEIPPPVPAS
                     QPQKGVSKWTPKEAPKEPVRPEKPPLAEGEVACEGCGLAITRNQEKLSRLLTGKSLCK
                     KCMNPP"
     gene            7660..8784
                     /locus_tag="VKO45_08420"
     CDS             7660..8784
                     /locus_tag="VKO45_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HMA05934.1"
                     /translation="MKKSLLMWDETLFREPEVFELDYVPEQFQHREDQMKELAFQVRP
                     GLKGARPLNTVCRGLPGTGKTTSVKKLFSEIEETTKKLVPVYVNCQIDNTKFAIFSQI
                     YRKLAGHLPPASGTSFKQVFDAISRIMLRDDIVLLVTLDDANYLLYENEMNRVLYTLL
                     RSHEAYPGTRIGVILIISDMTVDLLQAVDARVASVFRPTEVYFPPYREEEVVGILRDR
                     VNQGLYPNALPEPMFRLVVEQTMKSGDLRVGIDLLKRATLSAEKAAHRTIEREDVCRA
                     YEVSKYLHLAFTVKTLKAEERELLRKVAELSSGEKEMNAGEVYKALKEKESLGYTRFY
                     EMVKKLDAMRLVNLSYRDGRGRTRLISLRYDPGRVLEYLR"
     gene            8863..9810
                     /gene="mch"
                     /locus_tag="VKO45_08425"
     CDS             8863..9810
                     /gene="mch"
                     /locus_tag="VKO45_08425"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HMA05935.1"
                     /translation="MLSVNELAFDLFNELAEYPEDFNSAFHQLDNGARIVDCGVSTRG
                     GYQAGRIFTEICMGGLGEVGFRMGQIKGVPLPFIEVATDFPAISCLGAQKAGWTIKVG
                     NYTAMGSGPARALSLKPKHTYEVIDYEDDCEDAVICLESDHLPNGEVLEKIASECDVD
                     PSNTCAVVAPTASLVGSIQVAGRCVETAVYKLNELGFDTRTIVSGMGTAPIAPVKKDA
                     TRAMGSTNDATIYHGSIYLTVKNPKIKDFLEKIPSNKSKGYGKPFYEIFKEAKFDFYK
                     IDTSLFSPAEVVINELTEGVVYHVGAVNPDVTLKSFGFL"
     gene            10040..10113
                     /locus_tag="VKO45_08430"
     tRNA            10040..10113
                     /locus_tag="VKO45_08430"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10074..10076,aa:Val,seq:tac)
BASE COUNT         1889 a         3410 c         3205 g         1822 t
ORIGIN      
        1 tacgataccg gaacatggtg cctcctccat ccctctcttc acgatgcgtt gcaccccgga
       61 cgaaagaaat aaaactccgt ggtttaagat gcgggcgacc ttcgtgaggt ttttcgggaa
      121 tccccgcccg atcacgggtc atcttctttc gcgagccgaa agggtataaa ggagggtgtt
      181 caatccttcc acatcggaca agggtgaagg gatgcaggac ataccctcta tgatactcct
      241 cacttttctc ggaatcctga tcctcgtact ctttctcggc atggctcttc gccgggccga
      301 gaagctgcaa tcctggtacg aattcggtgg aaaggccggg accgttatcg gcatattcat
      361 cgttgccctc tttttcatgg aaaactttct cctcggtttc atcgccatgt gcatcgcttt
      421 ttacacgacc cggttcgtcg cggtcaagtt cttccagagg ctcgaggact tcatctgggg
      481 cccggaagcg gacccggcat gatccggccg atcctcatga tgggccggaa atccggatcc
      541 cgaacacacc agacagtacc accatgatcg accccgttga actcgctgag ctcgcagaca
      601 ggatcctcgc cattgccggg gagaaccaga agaccctcgc cgaggtgctg gacaccctcg
      661 atgcggaggc gcggagggag ctcctcgggt ctgacctcct gaacgccctg caggccttct
      721 attaccactt ccgggaaatg cccaccctcc tcgggcagga acgcctcacc ctgcagccgg
      781 catcggccgc cctcgagggg atcaccttcg aggaggagaa gggagtggat ctggtcttcc
      841 gcgtggagga gagcggaccg gtcgtcgagc tctccgagct cggggagatc atcgcgaagt
      901 tcgagggggc ctccgcgtac cggggggcga ggcgttacct ggaggattaa accgggtcgg
      961 gtctttcccc ctggtgatga tatccggatc cacccgtgcc ggaacgaggg gcaggggatc
     1021 cccccaggga aggatctcct cacccaggat gaccaccacg ccggttatat cagttccccc
     1081 gaggcgcccg aggacatccc ggttcccggg ggatatcttg ttgcagatgg cagtggccca
     1141 ggcgtccgcc gaggcgggat tttccgagaa gacggtgacc gagtcggcca cccccaggct
     1201 caccgaagga ccgacggtgg ccgacgatgt acagaccccc cggattcccc ctcccggcgg
     1261 gacgaggaag gcgaaccggt ccgagagggg tgaggaaccg gcgtggagcc cgatccggag
     1321 ttcgcgatca gtaacgaggg cgatatcccc tccgttgtcg atgacgccga agcgggcgcc
     1381 cgacccggcc atggcctcca ccccggacca ggcgatggca ccggccaccg ccgccatggg
     1441 cccgaccccg gccttcccgg cggcctctgc catccgcatc accaccttcc cgtcaccttc
     1501 cggtgtgtac ggatcatagg tgatggcaaa aaaggggtcc cgggcgatgt accgctccag
     1561 ctcctggcgc gcggacatca ttccctcctt tgctgccgta atatgggagg gatcgtctgc
     1621 gaggatggtc gcgatggtct cccggaactg gaaatgctca cgaatcatgg accctccccg
     1681 gctccctccg tcgttccctg tggaagtcct cctccagata tcgtcctcga ggcgtatatc
     1741 ggtgatcatg ggggcagaag gcttattgga tccccgcgac attgagggaa cgaaaagcca
     1801 tgaccgggga aaaacccaac cgcctcatcc atgaaaagag cccctacctc ctccagcatg
     1861 cgtacaaccc cgttgactgg tatccctgga gcgaggagcc cttccgccgg gcccgggccg
     1921 aggacaggcc cgtcttcctc tccatcggat actccacctg ccactggtgc catgtgatgg
     1981 cccgcgagtc cttcgacgac ccggaggtgg ctgccctcct gaatgcgagt ttcgtgaata
     2041 tcaaggttga ccgggaggaa cggcctgaga tcgatcaggt ctacatggag gtcgccctcg
     2101 ccatcatggg gacagggggc tggcccctca ccctcgtact gacccctgac gggaagccct
     2161 tcttcgccgc cacctacatt ccccggaaat cggggctcgg ccttgtgggg atgctcgatc
     2221 tcgtccccgg gatctcctca gcctggcgcg aaaggaggga agaactcctg gacgccgcgg
     2281 accgggtaac ggtcctcctc agatcccgtc ccccgtccgg tgatggcgga gatgccgtgg
     2341 cggtggtgga ccaggtcctc gacgacctga aggcgggctt tgaccgggag cacgggggat
     2401 tcggcggcgc cccgaagttc cccacgccgc acgccctgac cttcctcctc cgggaattcc
     2461 ggaaaagggc ggaccccgag ttgcttgcca tggcggagaa gactctcctc tccatgcatg
     2521 ccggggggat ctacgaccat gtcggttccg ggttccaccg gtatgccacc gatgcgggct
     2581 ggcgaatccc tcacttcgag aagatgctct acgaccaggc cctcctggcc atggcctaca
     2641 cggaggcctt cctgataacg ggaaaggccg tctaccggaa agtcgcggag gagaccctcg
     2701 gatacatgct ccgggatctc cggagtgcgg agggcgcctt tttcagcgcc gaggatgccg
     2761 agagcggtgg aagggaggga gggtattacc tctggacctg ggacggtatc ggggaggtcc
     2821 ttacgggaag cgaacgggcc gcctttaccg tctactacgg catctgcagg gacggaaata
     2881 tgccccggca tcccggggtg acagcctccg gggagaacgt cctcttcatc gccgatcccg
     2941 gcggggaggc tcccggggaa acgggtctcc cgcgggatgc cctcaccggg accctctccc
     3001 gggcgatgga gaaggtgcgc gcggcgagga accggcggca gcgcccgtcc cgggacgaaa
     3061 agatcctcac cgactggaac gggcttgcca tcggggccct cgccatggcc tcccgggcct
     3121 tcggctcggc agagcaccgc gaggccgccg gggagacggc caggttcatc ctcacccgcc
     3181 tcgccacaaa ggagggaaga ctgcttcacc ggtatttcgg cggtgaggcg ggggtggagg
     3241 ggaatgccga cgactatgcc ttcctggcct ggggactcat cgaactctac gagactacct
     3301 tcgacacccg tttcctcgag caggcggctg tcctcaccga tgtcctcctc tcccgtttct
     3361 gggaccggga gcaggggggc ttcttcttca cccccgatga cagcgaagcc ctcatcgcga
     3421 gactgaaacc ggtccacgac ggctcggtcc cgtccgcgaa ctccgtcgcc ctccacaacc
     3481 tgatcctcct cttccgcctc acgggcagga cccgttacct cgaccgggcg agggagctca
     3541 tggcatggta cctccgggag cgatcctcca cggccccggc ggcgacctgg atgatggcct
     3601 ccctctccct tgccttttcc ccgtccaccg aggtggtcat cgccggggac cccgatgccc
     3661 cggacacccg cgcactcctc gaggtcgcgg gcacgaggca gcatcccggg gtctcggttc
     3721 tcctgaaacg gccggccgga gatccgctcc tcgattccct tgctcccttc acccgggatt
     3781 tcaccacccg ggatgggatg gccgcggcct acgtctgcag gaaccatgcc tgcgagcttc
     3841 cggtcaccga tcccgagtcc ctcgcgagga tcctggatgc ccctccggga ccgggctcaa
     3901 aaggatgaag ggaatcccgg aatgtctttt atgagatcac gccccaatca ccttgaaaga
     3961 ggtccatccc ggtgaagaga tgacaggaag gcttctcacc gaacccgagg gatacgggct
     4021 gctcgaccgc tccggtatcc cggtgccccc ccacggggtt gcccggagcc ccgatgaggc
     4081 ggcccggatc gcggagggga tcggttttcc cgtggtcatg aagatcgtct ccccggatat
     4141 cgtccacaag agcgacacgg gaggcgtggt ccgggggatc cataacgccg ccgaggtgcg
     4201 cccggcctat caggagatcc tctcccgggt gaaggcgcgc gtcccggatg ccgccatcca
     4261 tggaatcatc atcgagaagg agcttccgcc ggggctcgag atcctcatcg gcgggaagac
     4321 cgacccgacc ttcgggaagg tcatcacctt cggcctcggg ggaaccctcg tggagatcct
     4381 cagggacgtg gccatccggc tcctccccct cgacctgaca gatgcgcagg acatggtgag
     4441 ggagatccgg ggctaccggc tcatcgcagg gtaccgcggg gaacccccga aggacgaggc
     4501 cgcgctcgtc tcccttctcc tcgccaccgc cgagatgttc gcgagggagg atatctcgga
     4561 gttcgacctc aacccggtta tcctgtatga gaaaggcgcc tgcgtcgtgg acgcccgtat
     4621 gtacctggga ggcgaggccc cggccgggcc ccggaagatc cactgtgcgc tcgcccccga
     4681 gatcttctcc ccggagtcca tcgccgtcgt cggggcatcg ggagatccgt caaaggtcgg
     4741 gtacgcggtg ttccggaacc tcctcccctt cccggggaga ctctacgcgg tgaacccgaa
     4801 ccggagcgag gtccagggga gggggaccta tccctccctc ggtgccctcc ccgaaccggt
     4861 ccacctcgcg gtcatcgcgg tccccgcccg ccttgttccc gggatcgtcg aggaggccgg
     4921 gaaggccggg gcgaaggctg ccatcgttct ctcggggggg ttcggggaga ccggcgcggc
     4981 gggaaaggcc ctcgaactcg aggtactcga gatcgcccgg aggtactccc tgcgcatcct
     5041 cgggccgaac tgcctcggga tcatgctccc ccacaagggg gtgaacacca ccttcgggcc
     5101 catcaccccg agaccgggcc gggtcgcctt catctcccag agcggggcgg tcatcgcgac
     5161 catggtggac tggtcggtgc ccgaggagtt cggtttctca gcggtggtga gcgtcgggaa
     5221 ccaggcagac ctcggcttcg aggacttcct cttttacctc gggggcgacc ccgagacccg
     5281 ggccatcgtc atgtacatcg aggaggtgaa ggacgggccg gccttcctcg aggccgtgga
     5341 gcgggcatct gcgagggtcc cggtcatcgc catcaagacc gggctctcgg caaaggggcg
     5401 ggcggcggcc tcctcccaca cgggggccct cgcggggagc cacgaggtgt acatggcagc
     5461 cttccggaag gccggcatca ccgcgaccca gtccctgcgc gaggccttcc tccttgcgga
     5521 gctcctcgct tccgagggat accccgcggg ccggaggggc atcgtcatca cgaacgccgg
     5581 gggctttgcg gtctttgcct ccgactatgc cgatctctac ggggtcgacc tcgtggacct
     5641 ccatcccgcg accctcacgg aactgaacgg atttcttccg gccagctgga gtcacgggaa
     5701 ccccctcgac cttgtcggcg actcgggggt cgatctctat gcccgggtct tcgatttcct
     5761 gatcaagcac caggagttct gggacatcgc cttcatcgtc tcggtccccg acgcggtcct
     5821 cgaccccacc cagctcgccc aggaggtggt ccgcttctcc cggaacacga agaagatggt
     5881 cgtcggctgc ttcctcgggg gttcctcggt cacggcgagc atccagattc tccggggcca
     5941 ccacatcccg aacttctccg acctggagga tgccttccgg gtcgtcggga gggcctgcgg
     6001 cctgaaaaag caggggaatc cccggaatcc ggggtaacgg gaagatattc aagcggactc
     6061 tcccaagggt actgggagca cggactcttt ctcggagata cctccatgaa gctactcctc
     6121 atcgagcggg atggcacgga tctctactcg accctctacg actcggacac gagccgggcg
     6181 atgctccggt tctatcaccc ggtccggcat cccgcgggtg tcgagatcca gacggcctcc
     6241 ctggggagcg ccctttccct cacctcggag ctcaggtggt acatccagcg ctacgtcgac
     6301 gaggtcctct tcaccgagga cggcacggtg tacctgacct ggtccctcgc cgatgaggtg
     6361 taccggcgcg gggccatcct ctccctgccc tggcctcacc ggttccgcta ccggatccac
     6421 ggaggaaagg tgtatcgctc tccctgggac ctcgccccct tcggggctcc tcctcccgaa
     6481 ccttcagcgg aagaggctgc tcttacaggg gtcgaggacc gggacctgat cctggaggtc
     6541 tggagtaccg cagaggagta cgcagagccc gaacgcgggg agccgatagg acccgcgggg
     6601 ccaccggtca ctgaagaggc gggggagcag ggggaaccac cggaaccgga gagccccggg
     6661 gaacccgggc cagtgcccct ctgagatccg ggccagtgtc tccgcaaggc cctgggacca
     6721 ccatccccca cacagatata ttcccgccgc ccatccttct atcatggcac ggaagatcct
     6781 caccatcggg atcaccatca accttgagaa ttacgagaac ctgcgcctgg aggtccaggg
     6841 ggagatcggg tcagacggga ataccgagga actcatctcc tttcttgacg gcatgcttgc
     6901 ccgcctcggc cacggggacc aggtgaccgc cgagcgggtc gaggcgtacc ggaggagggt
     6961 cctcgccatg cccggatccg cggtgatcgc ggctgtgccg gactggaagg ccgacgggac
     7021 ggaggtaccg ggtcctcgcc cggccccggt cgtgatccag gcagaacggg tcatcacccc
     7081 ccgcgaaccg caaccgccag agatcccgca ggaggcgccg ccaccgggag aggcacccgc
     7141 tgccccggct acagtcaggg tcgtatcggg aggtaggggg gcgaagccaa tctctcaggc
     7201 accggcgcgg cccgaggaga tacctccccc ggtccctgcc tcccagcccc agaaaggagt
     7261 gtcgaagtgg acaccgaagg aggccccgaa agagcccgtg cggcccgaaa agccccccct
     7321 cgccgagggc gaggtggcct gcgagggatg cggcctcgcc atcacgagga accaggagaa
     7381 gctctcccgg ctcctcacgg gaaagagcct gtgcaagaag tgcatgaatc ccccgtgagg
     7441 tcgcaccagg ctcaccacca ggacttcgcc cgttggttcc cgctcgggcc atggcccttc
     7501 gcgccttcgg cctgttgctc ctcgctcggg gctgtcgccc cttcacgttc accatctcgc
     7561 ttcgctcggt ggttctcgtt cggactaccg tcctcacgtt catcatcgcc ccgcgcaggg
     7621 tccaggttta agtatgaaac agatcgagtc tctagtggga tgaagaaaag cctcctcatg
     7681 tgggacgaga cgctcttccg tgaaccggag gtctttgagc tggactacgt ccccgagcag
     7741 ttccagcatc gggaggatca gatgaaggag ctcgccttcc aggtgaggcc ggggctcaag
     7801 ggggcgaggc ccctgaatac cgtctgccgg gggctgcccg ggaccggaaa gaccacctcg
     7861 gtgaagaagc tcttctccga gatcgaggag accacgaaga agctcgtgcc cgtgtacgtg
     7921 aactgccaga tcgacaacac caagtttgcc atcttctccc agatctaccg gaagctcgcc
     7981 ggtcacctgc cccccgcctc ggggacctcc ttcaagcagg tcttcgatgc catctcccgg
     8041 atcatgctcc gtgacgacat cgtcctcctc gtcaccctcg atgacgcgaa ctatcttctc
     8101 tacgagaacg agatgaaccg ggtcctctac accctgctcc gctcccacga ggcctacccg
     8161 ggcacccgca tcggggtgat cctcatcatc tccgacatga ccgtggatct cctccaggcc
     8221 gtcgacgccc gggtcgcctc ggtcttccgc cccaccgagg tctacttccc accgtacaga
     8281 gaggaggagg tggtgggcat cctccgcgac cgggtgaacc agggcctcta cccgaatgcg
     8341 cttccggagc cgatgttccg tctggtcgtg gagcagacca tgaagagcgg ggacctccgg
     8401 gtcgggatcg acctcctcaa gcgggcgacc ctctccgccg agaaggcggc ccaccggacc
     8461 atcgagcggg aggacgtctg ccgggcctac gaggtctcca agtacctcca cctcgccttt
     8521 acggtgaaga ccctgaaggc cgaggagagg gagctcctcc ggaaggtcgc cgagctctcg
     8581 agcggggaaa aggagatgaa cgccggagag gtgtacaagg ccctgaagga gaaggagagc
     8641 ctcggctaca cccggttcta cgagatggtg aagaagctcg acgccatgcg tctcgtgaac
     8701 ctctcctacc gcgacggccg gggaaggacc cggctcatca gcctccggta cgatcccggg
     8761 cgggtcctcg agtatctccg gtagggccgc gggatcgatt gaaaatcctt atcatatcct
     8821 catccctcac tactttcatc atctctaccg ggggaaaaca tagtgctcag cgtgaatgaa
     8881 cttgccttcg atctcttcaa tgagctcgca gaatatccgg aggacttcaa ttccgccttc
     8941 caccagctgg acaacggggc ccggatcgtg gactgcgggg tgtcgacccg gggcgggtac
     9001 caggcgggaa ggatcttcac cgagatctgc atgggggggc tcggcgaggt gggcttccgg
     9061 atggggcaga tcaagggggt gccccttccc ttcatcgagg tggccaccga ctttcccgcc
     9121 atctcctgcc tgggggccca gaaggccggg tggaccatca aggtggggaa ctacacggcc
     9181 atggggagcg gaccggcgag ggccctttcc ctcaagccca agcacaccta cgaggtcatc
     9241 gactacgagg atgactgcga ggacgcggtc atctgcctgg agagcgacca tctccccaac
     9301 ggcgaggtgc tcgagaagat cgcgagcgag tgcgatgtcg acccatcgaa cacctgcgcg
     9361 gtcgtcgcgc cgacggcctc ccttgtcgga tccatccagg tggccggccg gtgcgtcgaa
     9421 acggcggtgt acaagctgaa cgagctcggc tttgacaccc ggaccatcgt ctcggggatg
     9481 gggacggcac ccatcgcccc ggtaaagaag gacgcgacga gggccatggg ttcgacgaac
     9541 gatgcgacca tctaccacgg gtccatctac ctcaccgtga agaacccgaa gatcaaggat
     9601 ttcctcgaga agatcccgag caacaagtcc aagggctacg ggaagccctt ctacgagatc
     9661 ttcaaggagg ccaagttcga cttctacaag atcgacacct ccctcttctc tcccgccgaa
     9721 gtggtcatca acgagctcac cgaaggggtc gtgtaccacg tgggagccgt gaaccccgac
     9781 gtgaccctga agtccttcgg gttcttatag aacctggagg gtgactccga agtgagtcac
     9841 cccttcgggt ttttatgaga ttcggaaagt gatggcgaag tcatcactcc ttcgggtttt
     9901 tatgaagccg aagagtggct ccgaagtaag ccaatccttc gggttcccgt aaccttttcc
     9961 ttctctttcc tgccgccgtg aaacgggaag gcggtccttt gagcaatttt aataggatcg
    10021 ccgcaccacc ctaactggcg ggcttgtggt ctagttggtt atgatatcgc ctttacaagg
    10081 cggggatcgt cggttcgaat ccgaccaagc ccatggacgt ttttttcacg cggcgaaccc
    10141 ggtcgtggct gcctgcggtt gcgatgtgcg tcgtaagcca caggtgaggt ccggggcgaa
    10201 tcaccggccg gaaagcacta cccttattac ctgcgatacc gagaagctcc ctagagacac
    10261 gaggctggaa cactccatgg ctcccgaaaa gaaacctgtc ctcctcttca cgacctacaa
    10321 attccg
//
LOCUS       DATGDI010000223         4349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_83338, whole genome shotgun sequence.
ACCESSION   DATGDI010000223 DATGDI010000000
VERSION     DATGDI010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83338"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="VKO45_08435"
     CDS             complement(<1..1424)
                     /locus_tag="VKO45_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HMA05936.1"
                     /translation="MKCITHPLIRPESLEERRFQLTIALHALEGNTMVVLPTGLGKTA
                     IALIVAASRMYNKGGRVLVLAPTKPLVEQHLRFFEKFLIVPGRKEGDPSPFVMFTGET
                     PPEERRAGWAGSRLCFATPQAVKNDLLAGRYTLSDVTLLVVDECHRAVGNYAYVFIAR
                     RYNDTAADPLILAMTASPGGDRQKVEEVCANLSIARVETRVETDEDVRPYVHEREIHY
                     VPVDLPRELSEALQTLNRLVETRLAQLARFHFRVPKPGKLSIRALNELNAQIQTRIQA
                     RDRTAYQAASLYAEIMKLRHAISLAETQGSEALRHYLEKLAQEGAAPGGSKASQRLGS
                     DPAFRTLLAESSLWTGELHQKLELVGKLVQAQLSAHPDSRIIVFATYRDTVQNLVNHL
                     HSLGIQAERFVGQATKDAEKGLTQKKQIEALQRFRRGEFRVLIATSVGEEGLDVPSTD
                     LVIFYEAVPSEIRSIQRKGRTGRSG"
     gene            complement(1421..3118)
                     /gene="glyS"
                     /locus_tag="VKO45_08440"
     CDS             complement(1421..3118)
                     /gene="glyS"
                     /locus_tag="VKO45_08440"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HMA05937.1"
                     /translation="MELAKRRGFIWPSAEIYGGVAGLIDYGPLGAQMKRRVEEIWRSF
                     YVIREGFYEIECPTVGVEPIYVASGHVKGFSDKMVQCPHCEEYLRADHVAAANGCEGA
                     AAMTALTLEATLRSLPCSACGEELGEVKVFDFNLMFNTWIGPGSQRKGYLRPETAQGI
                     FTDFPRLLRFYRDRLPFGAVQVGKSYRNEISPRQGMIRLREFTQAEAEIFVHPEGKRH
                     PRFERYAEYRVPLLGCDGQDGKKEPALIIMREAVEKGIVANEYVAYYIALTHEILVTI
                     GVDPARLRFRQHLATERAHYAADCWDAEALSGRFGWVELVGIADRTDYDLRAHAGVSG
                     VALTAYIPLETPRMERRRRVIPDMKALGPKFRGKAKEVADALVQADPGPDGAEVTVGG
                     EKIAIPASLYQVREEEVEVRGVEVMPHVIEPSYGIDRMCYAVLEHSYGEDTVEGELRR
                     VLRLPSAVAPVQAGVFPLVSRDGLDRMATEIAAGLTRAGIFVEFDESGAIGRRYRRQD
                     EIGTPFAVTVDYQSIEDGTVTVRERDGMTQVRVKKDALAGVLSDLISGKITFSSLGP"
     gene            3278..3886
                     /locus_tag="VKO45_08445"
     CDS             3278..3886
                     /locus_tag="VKO45_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver protein"
                     /protein_id="HMA05938.1"
                     /translation="MEEDKRKRQLLDLFATITSKTKIVEPMKKIHGSLRDREAIEREI
                     SLIMREILDRGYFKTKLTPRQLSRLVMAFYDGKNDTEIARSLGDEKLSKTVARARVRM
                     KLFRDLDFKMPFDQKKLGKLVDSGKTMKDVSEELGISPSTLREYRHVLEEVNDPTLDP
                     FLTRIHDVMEDRDLSEQMTKGLAENGLTEAIDITEAELIDVT"
     gene            3931..>4349
                     /locus_tag="VKO45_08450"
     CDS             3931..>4349
                     /locus_tag="VKO45_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA05939.1"
                     /translation="MRLTWLGHSCFLLEGSRSVLIDPFVPEGPIRAEPDLVAVTHGHD
                     DHLGEVIRLGRKTVAISEIGKFLRSKGLPVETMNIGGTLTVDGVEFTMTQAIHSSGLE
                     AGGPGFYGGCSCGFVIGMDGVRVYHAGDTALFSDMKL"
BASE COUNT          725 a         1438 c         1428 g          758 t
ORIGIN      
        1 ccgctccggc cggttctccc cttcctctgg atggaccgga tctccgacgg gacggcctcg
       61 tagaagatga ccaggtcggt ggagggcacg tcgagcccct cctcccccac cgaggtcgcg
      121 atgagcaccc ggaactctcc cctgcggaac cgctggagag cctcgatctg cttcttctgg
      181 gtgaggccct tctccgcgtc cttcgtcgcc tggccgacga accgctctgc ctggatgccg
      241 agggagtgaa ggtggttcac caggttctgc acggtgtccc ggtaggtggc gaacacgatg
      301 atccggctgt cgggatgggc ggagagctgg gcctgcacga gcttgccaac gagctcgagc
      361 ttctggtgga gctccccggt ccagagggag gactcggcga ggagagtgcg gaaggccggg
      421 tcactcccga gccgctggga ggccttgctc ccgcccggcg cggccccctc ctgggcgagc
      481 ttctccaggt agtgcctgag ggcctcgctc ccctgggtct cggcgaggga gatggcgtgc
      541 cggagcttca tgatctcggc gtagagggat gccgcctggt atgccgtccg gtcccttgcc
      601 tggatccgcg tctgtatctg agcgttcagc tcgttgaggg cccggatgga gagcttcccg
      661 ggcttcggga cccggaagtg aaaccgggcc agctgggcga ggcgggtttc cacgagccgg
      721 ttcagggtct ggagcgcctc ggagagctcc ctcgggaggt cgacgggaac gtagtggatc
      781 tcccgctcgt ggacataggg ccggacgtcc tcgtcggtct ccaccctcgt ctccacccgg
      841 gcgatggaga ggttcgcgca gacctcctcg accttctgcc ggtcaccccc cggggaggcg
      901 gtcatggcga ggatgagggg atcggcggcg gtgtcgttgt accggcgggc gatgaagacg
      961 tacgcgtagt tccccaccgc gcggtggcat tcgtccacca cgaggagggt gacgtccgag
     1021 agggtgtacc tgccggcgag gaggtcgttt ttcaccgcct ggggcgtcgc gaaacagagc
     1081 cgggaacccg cccagcccgc cctccgctcc tccggcgggg tctcgccggt aaacatgaca
     1141 aagggggagg ggtccccttc cttccggccc gggacgatga ggaacttctc gaagaaccgg
     1201 agatgctgct cgacgagggg ctttgtcggg gcgaggacga ggaccctccc gcccttgttg
     1261 tacatccggg aggccgcgac gatgagggcg atggccgtct tcccgaggcc ggtggggagg
     1321 accaccatcg tgttcccctc gagggcatgg agggcgatgg tgagctggaa ccgcctctcc
     1381 tcgaggctct cgggccggat gaggggatgg gtgatgcact tcatgggccg agggaggaaa
     1441 aggtgatctt ccctgagatg agatccgaga ggaccccggc gagggcgtcc ttcttcaccc
     1501 tcacctgggt catgccgtcc ctctcccgga cggtgaccgt cccgtcctcg atgctctggt
     1561 aatcgacggt caccgcaaag ggagtcccga tctcgtcctg ccggcggtat ctccggccga
     1621 tggccccgct ctcatcgaac tcgacgaaga taccggcccg ggtgaggccg gcggcgatct
     1681 ccgtcgccat ccggtcgagc ccgtcgcggc tcaccagggg gaagaccccg gcctggaccg
     1741 gggccacggc ggacgggagc cggagcaccc ggcgcagctc gccctccacc gtgtcctcgc
     1801 cgtaggagtg ctcgaggacg gcgtagcaca tccggtcgat gccgtacgag ggctcgatga
     1861 catggggcat cacctccacg cccctgacct ccacctcctc ctcccgcacc tggtagagcg
     1921 acgcggggat ggcgatcttc tccccgccga ccgtcacctc ggcgccatcg gggcccggat
     1981 cggcctgcac gagggcatcg gccacctcct tcgccttccc ccggaacttc gggccgaggg
     2041 ccttcatgtc ggggatgacc cgcctcctcc gctccattcg cggggtttcg agggggatgt
     2101 aggcggtgag ggcgaccccg ctcaccccgg catgggcccg gaggtcgtag tcggtccggt
     2161 ccgcgatgcc cacgagctcc acccacccga acctccccga gagggcctcc gcgtcccagc
     2221 agtcggccgc atagtgggcc cgctcggtgg caaggtgctg ccggaaccgg agcctggcgg
     2281 ggtccacgcc gatggtgacc aggatctcgt gggtgagggc gatgtaatag gccacgtact
     2341 cgttggccac gatgcccttt tccaccgcct ccctcatgat gatcagggcg ggctccttct
     2401 tcccgtcctg gccgtcacat ccgaggagcg ggacccggta ctccgcgtac cgttcgaacc
     2461 tggggtgccg ctttccctcg ggatggacaa agatctccgc ctcggcctgg gtgaactccc
     2521 ggagccggat catcccctgc ctcggggaga tctcgttccg gtaggacttg cccacctgga
     2581 ccgccccgaa ggggagccgg tcccggtaga accggaggag acgggggaag tcggtaaaga
     2641 tgccctgggc cgtctcgggc cggaggtagc ccttccgctg cgagccgggg ccgatccagg
     2701 tgttgaacat gaggttgaag tcaaaaacct tcacctcccc gagctcctcg ccgcaggcgg
     2761 agcaggggag gctcctgagg gtcgcctcga gggtgagggc ggtcatggcg gccgccccct
     2821 cgcagccgtt cgcggccgcg acatggtctg cccggaggta ctcctcgcag tgcgggcact
     2881 ggaccatctt gtcggagaag cccttcacat gccccgatgc gacgtagatg ggctccacgc
     2941 cgacggtcgg gcactcgatc tcgtagaacc cctcccggat gacgtagaag gacctccaga
     3001 tctcctcgac cctccgcttc atctgggcgc cgaggggccc gtagtcgatg aggcccgcca
     3061 cgccgccgta gatctcggcc gacggccaga tgaagcccct cctctttgcg agctccatca
     3121 ccttctcgta gatgtcctcc ggttctgcca cgatgggatt ccctctcccc tgtacatcga
     3181 ccacaaatta taaaatcatg cttttccaag gtatgcggta aaggaattct taaataacat
     3241 ggcagagact gtagtgcgga aatcagcggg tacatccatg gaagaagaca aacggaagag
     3301 acagctcctc gatctttttg caaccatcac cagcaagacc aagatagtgg aacccatgaa
     3361 gaagatccat ggatccctca gggaccggga ggccatcgag cgggagatct ccctcatcat
     3421 gcgggagatc ctcgaccggg ggtacttcaa gacgaagctc accccccggc agctctcccg
     3481 gctcgtcatg gccttctacg acgggaagaa cgataccgag atcgcccggt ccctcgggga
     3541 cgagaagctc tcgaagaccg tggcccgggc ccgggtcagg atgaagctct tccgggacct
     3601 ggacttcaag atgccctttg accagaagaa gctcgggaag ctcgtggatt cggggaagac
     3661 catgaaggac gtgagcgagg agctcgggat ctccccctcc accctccggg agtaccgcca
     3721 tgtcctcgag gaggtcaacg acccgaccct cgaccctttc ctcacgcgca tccatgacgt
     3781 catggaggac cgggacctct ccgagcagat gacgaagggg ctcgccgaga acggcctcac
     3841 cgaggccatc gacatcaccg aggccgagct catcgacgtt acctgaagcc ccggcccaca
     3901 cctttttatc cctcctcccg attctctagt atgcgactca cctggctcgg ccattcctgc
     3961 ttcctcctcg aggggagccg gtcggtgctc atcgatccct ttgtcccgga gggccccatc
     4021 cgggcggagc cggatctcgt ggccgtcacc cacggccatg acgatcacct cggcgaggtg
     4081 atccggctcg ggagaaaaac ggtcgccatc tcggagatcg ggaagttcct caggtcaaag
     4141 ggcctccccg tggaaacgat gaacatcggc gggaccctca cggtggacgg ggtggagttc
     4201 accatgacgc aggccatcca ctcctcgggg ctcgaggccg ggggcccggg gttttacggc
     4261 ggctgttcct gcgggttcgt catcgggatg gacggggtgc gggtttatca cgccggcgac
     4321 accgcactct tctcggacat gaagctcat
//
LOCUS       DATGDI010000224         2547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_373987, whole genome shotgun sequence.
ACCESSION   DATGDI010000224 DATGDI010000000
VERSION     DATGDI010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373987"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2547
                     /locus_tag="VKO45_08455"
     CDS             <1..>2547
                     /locus_tag="VKO45_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HMA05940.1"
                     /translation="QTNFPVLVSLPFDSDLAASAQANGNDIVFTDSGGSTVLPHEIEN
                     FTKSSGALTAWVKLPTLSNGVNTDLYMYFGNSSASNQQNSTAVWDSDYVGVYHLKESG
                     NGTAGEYIDSTYQNPGQGGQGLAAYVPKRVAGKIGYGQNFDNLVDGKWDLIDLHNTSS
                     LLSIDSGNQITVEAWIQPSFYQGDLVNQTRGFINHKGWFSGYSIVMNNWGCSSPNCVW
                     FGLPGQNHSVQTSSVVSKDNWHHVAGTYNGSWAEVLIDGQVDSNKHAMSATFIPPNPP
                     FNEVWIGYGDQPTDTGYASEWWGLIDEVRISRIGRSESWIGTEYNNQNRPDLFVSRGT
                     TGAGCGGGGGPLVINPCAWASRKKLTINGTMISGTQANFPVLVSLSSDSDLAAHAQAS
                     GNDIVFTDSGGSTVLPHEIENFTKSSGALVAWVKLPAISASVNTDIYLYYGNSSVGSQ
                     QNVTGVWDADFKGVWHMKEDVAGTGTADVYQDSTSNNNDADDYISATGQSGKIGAGHQ
                     FDGTDDQANVPDPGGSWEFANGGLDAGLSDYSISAWIWLSSSYSGNYPTPVAKGGGAD
                     NDPGYWIDYYRTEDRIDNRLSDGIHSRFIANSSPSLGLTKDTWHHIYAVMDRAAGTDT
                     AYFYLDGNSVGSQSSTNVAGNSATGYMGFYIGSYSSTGAPWMGNLDEVRISSTVRSSQ
                     WIATEYKNQNRPDLFVSKGTEENGCGGIPLVFSPCSWTSRKLLTTNITMINGTQSNFP
                     ALVSLSSDADLAADAQDNGNDIVFTDSTGTQIPHEIESFSGTTGALVAWVKLPTVSSS
                     VNTDIYMYYGNSSTASQQNASGVWDSNYRGVWHMNVTADSTSRNTCTNQGS"
BASE COUNT          547 a          832 c          699 g          469 t
ORIGIN      
        1 ccagaccaac ttcccggtcc tcgtcagcct ccccttcgac tccgacctcg ccgccagcgc
       61 ccaggcgaac gggaacgaca tcgtcttcac cgactccggc ggctccaccg tcctccccca
      121 cgagatcgag aacttcacga agtccagcgg cgccctcacc gcctgggtga agctcccgac
      181 cctgtccaac ggggtaaata ccgacctgta catgtacttc gggaactcgt ccgcctccaa
      241 ccagcagaac tccacagccg tctgggacag cgactacgtc ggcgtctatc acctgaagga
      301 gtccgggaac gggaccgcgg gcgagtacat cgacagtacc taccagaacc ccggccaggg
      361 cgggcagggt ctcgcagcct acgtacccaa gcgggtggca gggaagatcg ggtacggcca
      421 gaacttcgat aacctggtcg acgggaaatg ggacctcatc gacctccata acaccagttc
      481 cctcctctcc atcgattccg ggaaccagat caccgtcgag gcatggattc agcccagttt
      541 ctaccagggg gacctggtga atcaaacccg cgggttcatc aaccacaagg ggtggttctc
      601 cggatatagt atcgtcatga ataactgggg atgctcctcg ccaaactgcg tctggttcgg
      661 cctgccggga cagaatcaca gtgtgcagac cagttcggtt gtgtcgaaag ataactggca
      721 ccatgtggcc ggcacctaca acggatcctg ggcagaggtc ctgatcgacg gacaggtgga
      781 ttccaataaa cacgccatgt cggccacctt cataccgccc aatcctccgt ttaacgaagt
      841 atggatcggg tacggcgatc agccgacaga tacggggtat gcctccgagt ggtgggggct
      901 catcgacgag gtccggatct cccggatcgg ccgttcggag tcctggatcg ggaccgagta
      961 taacaaccag aaccgccccg acctcttcgt ttccaggggg acaacgggag ccggctgcgg
     1021 aggcggcggc gggccgctgg tcatcaatcc gtgcgcctgg gcctcgcgaa agaagctcac
     1081 catcaacggg acgatgatct ccgggaccca ggccaacttc ccggtcctcg tcagcctctc
     1141 ctcagactcg gacctcgccg ctcatgccca ggcgagcggg aacgacatcg tcttcaccga
     1201 ctcgggcggg tcaaccgtcc tcccccacga gatcgagaac ttcacgaaat cgagcggcgc
     1261 cctcgtcgcc tgggtgaagc tcccggccat ctcggcctcg gtgaacaccg acatctacct
     1321 ctactatggc aactccagcg tcgggagcca gcagaatgtc acgggggtct gggacgcaga
     1381 tttcaaaggc gtctggcaca tgaaggaaga cgttgccggc accggaaccg cggacgtcta
     1441 tcaggactcc acctccaaca acaacgatgc cgatgattac atctccgcca caggtcagag
     1501 cggtaagatt ggtgccggtc accagttcga cggtacggat gatcaggcta atgttcccga
     1561 tcccgggggc tcgtgggaat ttgccaatgg aggcctggat gccggcttga gcgactattc
     1621 catctcggcc tggatctggc tctcgtcctc ctactcgggt aattacccca cccccgttgc
     1681 caaaggggga ggagccgaca acgacccagg ctactggatt gattattatc gcaccgagga
     1741 ccggatcgat aatcgccttt ccgacgggat ccattcacgt ttcattgcaa attcatcccc
     1801 ctcactaggt ctcaccaagg atacctggca ccacatatac gccgtcatgg accgggcggc
     1861 ggggactgat acggcatact tctacctgga cgggaactcg gtcggaagtc agagttcaac
     1921 caatgtcgcc ggtaactccg ctacgggcta tatgggattc tacatcggat cctattcttc
     1981 caccggtgca ccatggatgg gaaaccttga cgaggtccgc atctccagca ccgtccgctc
     2041 ttcccaatgg atcgcgaccg agtacaagaa ccagaaccgc ccggacctct tcgtttcgaa
     2101 ggggaccgag gagaatgggt gcggtggcat ccccctcgtc ttcagcccct gctcctggac
     2161 ctcccggaag ctgctcacga ccaatattac catgatcaac gggacccagt ccaacttccc
     2221 ggccctggtt agcctctcct cggatgcgga tctcgccgcc gatgcccagg acaacgggaa
     2281 cgacatcgtc ttcaccgatt cgacggggac ccagattccc cacgagatcg agagcttctc
     2341 gggcaccacc ggagccctcg tcgcctgggt gaaactcccg accgtctcct cctcggtgaa
     2401 caccgacatt tacatgtact acgggaactc ttccacagcc agtcagcaga acgcaagcgg
     2461 cgtgtgggat tcgaactaca ggggtgtctg gcacatgaac gtgactgccg acagcacttc
     2521 ccgcaatacc tgtaccaatc agggttc
//
LOCUS       DATGDI010000225         1516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_286928, whole genome shotgun sequence.
ACCESSION   DATGDI010000225 DATGDI010000000
VERSION     DATGDI010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286928"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="VKO45_08460"
     CDS             complement(<1..1430)
                     /locus_tag="VKO45_08460"
                     /inference="COORDINATES: protein motif:HMM:NF019407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HMA05941.1"
                     /translation="MDVRDRAVSEIIGTLVLVGIVVIGIVLAAILLFSNPAASRVPIF
                     DAIISNQSNTIYIYHKGGDSLIAGEYRILVDGVDRTSSFVNNGDEPWSVGETLSYTSP
                     TMPGHVVIVFTGVGQGGGSVIGQADLVPVLEVPAHLPNYPLVTWSSSPVFGNITTPFQ
                     FTETSSVDNLTSYFWNFNDANTSTVKSPSHVFPCAQEYCTYEIDHGVTDSGGTGWAAT
                     TWVNRSAWITVFQNLTPTVTFTNTSATGQVSLTVTFNATPAGAIRVDSWYWTFGDGGT
                     STSRNATYTYTTAGHYNVTLTATNYTLGVTTVTRSNLVTAFPNPSWYHCSWGYRKNIT
                     IDHNRVAADLVNFPVLINLASDADLSANARADGFDILFTSSTGTTKIPHEIENFTKSS
                     GALVAWVNVSALSSTTDTVLYLYYGNPSSGDQQSRTSVWDSNYKAVWHLGELVTDEAT
                     TGSHLDSTSNANTALQRKNGPIGGKIG"
BASE COUNT          292 a          405 c          508 g          311 t
ORIGIN      
        1 ccgattttgc caccgatggg gccgtttttc ctctgcaggg cggtgttcgc attcgaggtc
       61 gagtcgaggt gagaaccggt ggtggcctcg tcggtcacga gctcgccgag gtgccagacg
      121 gccttgtagt tcgagtccca gacgctcgtc ctgctctgct ggtctcccga ggagggattc
      181 ccgtagtaca ggtaaaggac ggtatccgtg gtggaggaga gggcggagac attcacccag
      241 gccacgaggg ctccgctgga tttcgtgaag ttctcgatct cgtgcggaat cttggtcgtc
      301 ccggttgacg aggtgaagag gatatcgaag ccatcggccc gggcgttcgc agaaaggtca
      361 gcgtccgagg caaggttgat gaggaccggg aaattgacca ggtctgcagc cacccggtta
      421 tggtcgatgg tgatgttctt ccggtacccc cagctgcagt gataccacga ggggttgggg
      481 aacgccgtga caaggttcga tctcgtcaca gtggtaacgc ccagggtata attcgtggcc
      541 gtcagcgtga cgttgtagtg ccctgccgtg gtataggtgt aggtggcatt ccgggaggtg
      601 gaggtccccc cgtccccgaa agtccagtac caggaatcca cccggatggc acccgcaggg
      661 gtcgcgttaa aggtcaccgt gagagagacc tggcctgtcg ccgaggtatt ggtgaaggtc
      721 acggtggggg tcaggttctg gaacaccgtg atccacgccg aacggttcac ccaggtggtg
      781 gccgcccagc ccgtccctcc cgaatcggtg accccgtggt cgatctcgta ggtgcagtac
      841 tcctgggcac aggggaagac gtgagagggc gatttcaccg tcgaggtgtt ggcatcgttg
      901 aagttccaga agtacgaggt gagattatcg acggaggaag tctcggtgaa ctgaaacggg
      961 gtggtgatat tcccgaaaac cggggagctg gaccatgtca ccagcgggta gttgggcagg
     1021 tgggcgggaa cttccaggac gggaaccagg tctgcctgtc cgatgacaga tcccccgccc
     1081 tgaccgaccc cggtgaacac gatgaccacg tgcccgggca tcgtcggtga cgtatagctg
     1141 agtgtttccc cgactgacca gggctcgtcc ccgttgttta cgaaggagga ggtccggtcc
     1201 accccgtcca cgaggatcct gtactccccc gcgatgaggg aatcgccgcc cttgtggtag
     1261 atatagatgg tgtttgactg gttggagatg atggcgtcga agataggaac cctggaggcc
     1321 gcgggattcg agaacaacag gatggccgca agcacgattc cgattaccac gatcccgacg
     1381 aggaccaggg tcccgatgat ctcggagacc gcccgatccc ttacgtccac ttccattcct
     1441 cgggttgctt catgccgggg gggccttcct gggctaagca aataagattg aacgatttct
     1501 gattcggggt gttctt
//
LOCUS       DATGDI010000226         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_83427, whole genome shotgun sequence.
ACCESSION   DATGDI010000226 DATGDI010000000
VERSION     DATGDI010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83427"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            470..1003
                     /locus_tag="VKO45_08465"
     CDS             470..1003
                     /locus_tag="VKO45_08465"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMA05942.1"
                     /translation="MAGKGGQGEADRREGIYSLISSLGEQCEYAERIRAATALGESRD
                     PRAISPLVECLKDVDPRVRRTCAEALLRLGSIRSVQAFIERLEDGEESVFTRKVAADA
                     LAKIRTYSAIESLASRLLDRSEKEAIRIHAADALGRTGSLRAIDALEKGTEDENPRVR
                     QESGKALSQIFAFGPGG"
     gene            1350..2381
                     /locus_tag="VKO45_08470"
     CDS             1350..2381
                     /locus_tag="VKO45_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05943.1"
                     /translation="MIASCDTVTDECVYTPAADTTVCDDGNACTVSDACTGGVCAGTP
                     AADTTVCDDGNICTVNDVCTGGVCTGTSLPEHTACDDGNSCTTNDACVEGFCFGMSLD
                     PGAACASDGNPCTNDVCRSLEGKSACFHRAVADGTVCDVGLPCTIGGVCSAGVCTGSG
                     FAARGTPCEADKPCTDGICNGAGTCLDEPVPAGEPCHGGSCDLSGECIPLKCDPVPMN
                     YLWPPNHKFVPVTLTVPGAAESGVTVDSVKSSEPPSVKGAGGKSKEPDAYLSKTDPLT
                     VFLRSERSGLDKGKSSGLGRLYTISYSATNNLDTKCKGIVKVCVPHDQSAQVKASSCQ
                     DSGAKYNAL"
     gene            complement(2556..>3111)
                     /locus_tag="VKO45_08475"
     CDS             complement(2556..>3111)
                     /locus_tag="VKO45_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HMA05944.1"
                     /translation="PAGGVPVGVAVSRALACPLHPLIVRKVQIPWNPEAGFGAVAWDG
                     RVFLNKELLAQLHLTPGQVEAAVRRAQANVRERVARFGSTFPDPAGRSCIIVDDGLAS
                     GFTMTAAAEALRSLGPRKVIVAVPTGSHAAVERVAKLVDWVVCLNIRTGYSFAVADAY
                     EDWYDLSEEEAMGMIRGAVSPGHT"
BASE COUNT          626 a          948 c          967 g          570 t
ORIGIN      
        1 agggcatcct gggcgactac aatgaaggga ttgacggccc cggccattgc cagtcgtgaa
       61 cggacttcct tccgaaattc ctctttttat tgctgaagat ctgtgcctgc ctggtgttca
      121 ttttctgtcg cttctgttct cctgggattt gaggaagatt tccctgtcgg cagaatatga
      181 aaccgtcctc atacgctcaa acggcagaac cattccagtt cctaaccctg aagatagcct
      241 gccttcatgc aaccgatggc tccacgccgc aaattattaa tacaaaaagc cccaagggaa
      301 taccgatgct cttgtcagcg tccatcctgt gccatctcgg gcatgtagcg acgactggta
      361 agtggcagct cttcttcccg tgctgaagca tccttcggtt ctgccggatg atggcggcgc
      421 ggattctctc gaagaatcgt ctgggaaggt ctgttggggt atgaacgcca tggctggaaa
      481 agggggccag ggggaagccg accggaggga gggcatctac tccctcatca gctccctggg
      541 agagcagtgc gagtatgcag aacggatccg ggcggcgacc gccctggggg agagcaggga
      601 cccgagggcc atctcgcccc tcgtcgagtg cctgaaggat gtcgacccga gggtgcggag
      661 gacctgcgcc gaggccctcc tccggctcgg gtccatccgg tcggtgcagg ccttcatcga
      721 gcgcctggag gacggggagg agtcggtctt cacccggaag gtcgcggccg atgccctcgc
      781 aaagatccgg acctacagcg ccatcgagtc cctggcgagc cggctcctcg accgttcgga
      841 gaaagaggcc atccggatcc atgccgccga tgccctcggg aggacgggca gcctccgggc
      901 catcgatgcc ctggagaagg gtaccgaaga cgagaacccc cgggtgaggc aggaatcggg
      961 aaaggcactt tcccagatct ttgccttcgg accgggaggg tgaggggtgc ggcacgtcct
     1021 taccggcgag agagagaggg aaatggaaga gcgggaaggg agcggcagtc tcggggcagg
     1081 ccctgagatt aattttttct cttgaacaaa aaggaagatc cttccctttt atcccatcac
     1141 gatccttaaa cccaagattt taatatctgc ccgtcgtatt ctccgtccga cgcgtgtccc
     1201 tggggaaccg ggacatcgcc cggataaaaa gggggaaaaa catgaagaaa ctgtttctag
     1261 catgtgcttt cctgctgtgc atcctcttcc tggcagggcc tgcagcggca caatgcgacc
     1321 cggaaacatg tgtatcaagc aacctgtgcg tgatcgcctc atgtgataca gtcacggatg
     1381 agtgcgtcta cacacccgcg gctgacacca cggtctgtga cgacggtaat gcctgcaccg
     1441 tcagtgatgc ctgcaccggc ggagtctgcg ccggaacacc tgctgcagac accacggtct
     1501 gtgacgacgg caatatctgc accgtcaatg atgtctgcac cggcggagtc tgcaccggaa
     1561 cgtctttgcc cgaacacacg gcctgcgacg atggcaattc ctgcaccacc aatgatgcct
     1621 gtgttgaagg attctgtttc gggatgagcc tcgatcccgg cgcagcctgt gcgtcggacg
     1681 gcaacccctg caccaacgat gtatgccggt cgctggaggg gaagtccgcc tgcttccacc
     1741 gggccgtggc tgatggcaca gtctgcgacg ttggcctgcc ctgcaccatt ggcggtgtct
     1801 gttccgctgg tgtctgcacc ggatccggtt tcgctgccag gggaacgccc tgtgaggccg
     1861 acaaaccgtg caccgatggt atctgcaacg gggcaggcac atgcctggat gaacccgtcc
     1921 cggccggcga gccgtgccat gggggttcat gcgatctgtc gggggagtgt atccctctga
     1981 aatgtgatcc cgtgcccatg aactacctct ggccgcccaa tcacaagttc gtccccgtga
     2041 cccttacggt cccgggggcc gcggaatcgg gagtcaccgt tgacagcgtg aagagcagcg
     2101 agcccccctc cgtcaagggg gccggcggga agtcgaagga accagatgcg tatctcagta
     2161 agaccgatcc cctgacagtc ttcctccggt ccgagcgttc aggtctcgac aaggggaagt
     2221 caagcgggct cggcaggctc tacacaatct cctactccgc cacaaacaac cttgatacga
     2281 aatgcaaggg aatcgtgaag gtgtgtgtcc cgcacgacca gagcgcacaa gtgaaggcat
     2341 ccagctgcca ggacagcggg gcgaaataca acgcactgta aaagaatacg tgaagctgcg
     2401 taatcccgaa tagaaaggca cacgcccccg gacgggaacc caccggggtt tcccttcccc
     2461 cttttccttc tttctcgagc cccggattca ccgtatcagg gaccgaaggc cggttatggc
     2521 cgccgcccct gggcggaagc cgtcgaagag cccgcctatg tgtgtcccgg gctcacagca
     2581 cccctgatca tccccatcgc ctcctcctct gaaagatcgt accagtcctc gtaggcatcg
     2641 gcgaccgcga aggagtaccc ggtcctgatg ttcaggcaga ccacccagtc aacgagcttc
     2701 gcgacccgtt ccaccgccgc gtgggagccg gtcgggaccg cgacgatgac ctttcggggg
     2761 ccgagggatc gaagagcctc ggcggcagcg gtcatggtaa agcccgaggc aagcccgtcg
     2821 tcaacgatga tgcaggaacg gcctgcggga tcggggaagg tggagccgaa gcgtgccacc
     2881 cgttccctga cgtttgcctg cgcccggcgc acagcggcct cgacctgccc gggagtgagg
     2941 tggagctggg ccaggagttc cttgttcagg aagacccggc catcccaggc gaccgccccg
     3001 aacccggcct cggggttcca ggggatctgg accttccgta cgatcagggg atgcaggggg
     3061 caggcgaggg cccgggaaac cgcgacgccg acggggacac cgccggcggg g
//
LOCUS       DATGDI010000227         3709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_403476, whole genome shotgun sequence.
ACCESSION   DATGDI010000227 DATGDI010000000
VERSION     DATGDI010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403476"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VKO45_08480"
     CDS             complement(<1..942)
                     /locus_tag="VKO45_08480"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HMA05945.1"
                     /translation="MRYFENIVETIGNTPLVRLRNVLPEGTRPLVLAKLESMNPGGST
                     KDRIAANMLLQAEKAGLIRKGGTVVEPTSGNTGAGIALVAASRGYGMVFTMPEKMSKE
                     KEILLRAYGAEVIRTPTEAPPEDERSYYKVAARVARETPGSFVPNQFENLNNPSAHYL
                     TTGPEIWRDTEGKITHFVAGIGTGGTISGTGRYLKEQNPKIRIVGVDPEGSIYHHVFY
                     GTPPVAHLYKVEGTGEDFLPKTLDLGVVNEVITVEDRDAFRMTRRLAREEGLLVGGSA
                     GASVFAASRLAETLGEDDVVVVLLPDTGRNYLSSIFND"
     gene            complement(952..1311)
                     /locus_tag="VKO45_08485"
     CDS             complement(952..1311)
                     /locus_tag="VKO45_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05946.1"
                     /translation="MGCGDVGSWFRSLGWYGYLLVGIFIVIAGYFIYVHQAHLAAYSG
                     IILIVLFIGVHFLACGRGHGSHGDQGGGCCGGGSHGNHGDHGKKEEEKAGAGTPSDTG
                     KDEDEKEKGKEKGGSCH"
     gene            complement(1340..1549)
                     /locus_tag="VKO45_08490"
     CDS             complement(1340..1549)
                     /locus_tag="VKO45_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05947.1"
                     /translation="MVDHTVSWNFTIVKCFAPGWIGDTVIWLKNDKTRFAGIMNTGVS
                     KEGIQLIKRQRLPESILSMVYPLDL"
     gene            1552..1836
                     /locus_tag="VKO45_08495"
     CDS             1552..1836
                     /locus_tag="VKO45_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMA05948.1"
                     /translation="MIVFVVNDTTLVSDARKIEHLPPSAKLVYTILKANRQMTQKDLI
                     RETYLPPRTVRYALSRLKRENVLVERLHITDARQSLYGLRQVMHGMAIEA"
     gene            2099..2170
                     /locus_tag="VKO45_08500"
     tRNA            2099..2170
                     /locus_tag="VKO45_08500"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2131..2133,aa:Cys,seq:gca)
     gene            2425..2970
                     /locus_tag="VKO45_08505"
     CDS             2425..2970
                     /locus_tag="VKO45_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05949.1"
                     /translation="MKLLPVLAAAFLLLLGIVPAASAHCPLCTAGAALGVGVARAYGV
                     DDSIVGLLLGAFVASSGLWIDKLLKRRGIEYPLQAPLLVLGSLLLLAVPLYMTGTITN
                     VGVVRALPGYPSIFGLGALGLDKLFAGLLIGTFLVAGVFTMSDRIIEKRGGKRLFRYQ
                     GMILMAISVAIFAVILWAMTR"
     gene            3026..3430
                     /locus_tag="VKO45_08510"
     CDS             3026..3430
                     /locus_tag="VKO45_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05950.1"
                     /translation="MIFLVIFAAGAVLFVIAGKGETSPAPRQGITSPQKGSNLFRGNI
                     VDSGASPGSFTGVPVISDEGCQTDPKNGLSNCTAGVKTPDAGILEFNYEHNMMVKPCL
                     TQGDIVSLQENTDGTAMVTRTHWTGGGGARAQ"
     gene            3456..>3709
                     /locus_tag="VKO45_08515"
     CDS             3456..>3709
                     /locus_tag="VKO45_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05951.1"
                     /translation="MYTLVALILTFSALVLVGIPAGKDKPRGRISTTSSPSVIYLSQL
                     ERDVIPAEGFTVPAKWGTGVSELVNSRAIDSSSFFSSMMGA"
BASE COUNT          701 a         1161 c         1070 g          777 t
ORIGIN      
        1 gtcgttgaag atgctgctca ggtaatttct cccggtgtca gggaggagca ccacgacaac
       61 gtcgtcttct cccagggtct ccgcgagcct tgaggccgcg aagaccgagg cccccgccga
      121 tccgccgacg aggagcccct cctcccgggc gaggcgtctc gtcatccgga aggcgtcccg
      181 gtcctcgacg gtgatcacct cgttcacgac cccgaggtcc agggtcttcg ggaggaagtc
      241 ctccccggtc ccctctacct tgtacaggtg ggcaacgggg ggagtcccgt agaagacgtg
      301 gtggtagatg gacccttcgg ggtcaacccc cacgatcctg attttagggt tctgttcctt
      361 caggtaccgg cccgtccccg agatggttcc gccggtcccg atcccggcca cgaagtgagt
      421 gatcttcccc tccgtatcgc gccagatctc cgggccggtt gtcaggtaat gggcactggg
      481 attgttcagg ttctcgaact ggttggggac aaaggacccg ggcgtctcgc gtgccacccg
      541 ggcagcaacc ttatagtagc tccgttcgtc ctccggggga gcctccgtag gggtcctgat
      601 cacttcggcc ccgtatgccc ggaggaggat ctccttctcc ttactcatct tctcgggcat
      661 ggtgaagacc atcccgtacc cgcgggaggc ggcgacgagg gcgatcccgg cgcctgtgtt
      721 ccccgacgtg ggctcaacga cggtaccccc cttccggatc aggccggcct tttccgcctg
      781 gaggagcatg ttcgcggcga tccggtcctt ggtgctcccc ccggggttca tgctctccag
      841 tttcgcaagg acgagcggcc ttgtcccctc cgggagaacg ttccgcagcc tcacgagggg
      901 ggtattcccg atggtctcaa cgatgttttc gaagtacctc attccacgac ctcaatggca
      961 gctcccgccc ttctccttcc ctttctcctt ctcatcctca tcctttcctg tatccgatgg
     1021 ggtgcctgcc ccggccttct cctcctcctt ctttccatga tcaccgtgat tcccgtgcga
     1081 tcctccgccg cagcaaccgc cgccctggtc cccgtggctt ccatggcccc tcccgcaggc
     1141 caggaagtgg acgccgatga agaggacgat gaggatgatc ccggagtatg ccgcgaggtg
     1201 tgcctggtga acgtagataa agtaacccgc gatcacgatg aaaatcccca cgaggaggta
     1261 cccgtaccag cccagcgacc tgaaccagct gccaacgtct ccgcatccca tgatttgcat
     1321 cacttccctt tcttcgactt cacagatcaa gcggataaac catagaaagt atggattcag
     1381 gcaacctctg cctcttgatg agttgaattc cctccttgct cacccccgtg ttcatgatgc
     1441 ctgcaaagcg cgtcttgtca tttttaagcc atataacagt atctcctatc caccccggag
     1501 cgaaacactt aacaattgtg aagttccatg atactgtgtg gtcaaccatg agtgatcgtc
     1561 tttgtggtca atgacaccac cctcgtaagt gatgcacgga agatcgaaca cctgccgcct
     1621 tcggcaaagc tcgtgtacac gatcctgaag gcgaacaggc agatgaccca gaaggacctc
     1681 atccgcgaaa cctatctccc gcccaggacg gtgcggtacg ccctctccag gctgaaaagg
     1741 gagaacgttc tcgtggagcg ccttcacatc accgatgccc ggcagagcct gtacgggctc
     1801 cggcaggtta tgcacgggat ggcgatcgag gcataagaat ccacccgatt tccccacttt
     1861 tcccttcctt cactctccat ggaactgtgt ctcattaccg atccctttat tcgggattgc
     1921 cggttgcagt aaacgagggg gtagcattcc tggcagagag tccatccggg ggcccccggt
     1981 tcatccgagg gtctgcgatg ttctatccgg attgcggaaa aacttgcctg aaaatcagcg
     2041 tataaaagcc atgcacgagg aaccaagtac gtgatctcac aggggtccga taatgattgc
     2101 caaggtggcg gagcggctac gcggctgact gcagatcagc tcaatcccgg ttcgaatccg
     2161 ggccttggct tacgggcggg cggccatcag gacggctgcc cgtggagaaa atcggcgaag
     2221 ccgattttcg actggcgggg ttcgatagaa gcccgccgga acgagggcac tcatcagagt
     2281 ggccggtcgt ggagaagatt ggcgaagcca atcttcgact tctacccgac aggcggccgc
     2341 aaggacggtt gcctgtggag aaaatcggcg aggccgattt tcgaccatca ggagggcggc
     2401 ggccgaaaga atggccggtc atgggtgaaa ctcctcccgg tcctcgccgc ggcctttctc
     2461 ctcctcctgg ggatcgtccc cgccgcctcg gcccactgcc ccctgtgcac ggccggagcg
     2521 gcgctcggag taggcgtggc gcgtgcgtac ggggtcgatg acagcattgt cgggctgctt
     2581 ctcggggcct tcgtcgcctc gtccgggctc tggatcgaca agctactgaa gaggcgggga
     2641 atcgagtatc ccctgcaggc ccctctccta gtcctgggct ccctcctgct gctcgccgtc
     2701 cccttataca tgaccggaac catcacgaac gtcggggtgg tgcgtgccct ccccggctac
     2761 ccctcgatct tcggcctcgg ggctctcggg ctcgacaagc tctttgccgg cctgctcatc
     2821 ggcaccttcc tcgtcgccgg ggtcttcacg atgagcgatc gcatcatcga gaagaggggt
     2881 gggaaaaggc tgttccggta ccagggcatg atcctcatgg cgatctccgt cgcgatcttt
     2941 gccgttatcc tctgggcaat gacgaggtaa gggagggaga cggatgaaaa aagattccgg
     3001 gttattgtct gccctggaaa tcggtatgat attcctcgtc atattcgcag cgggagcggt
     3061 cctgttcgtc attgcaggaa agggcgagac atcgccagcg ccgaggcagg gcatcacgag
     3121 cccccagaaa ggatcgaacc tcttccgggg aaacatcgtg gattccggag cgagccccgg
     3181 gtcttttacg ggggttcccg tcatctccga cgaaggatgc cagaccgacc cgaagaatgg
     3241 tctctccaac tgcaccgccg gcgtaaagac cccggatgca ggcatcctgg aattcaatta
     3301 tgagcataac atgatggtca agccctgcct cacacagggc gacattgtct cgcttcagga
     3361 gaataccgat gggaccgcaa tggttacgag aacgcactgg acaggaggag ggggagcacg
     3421 tgcccagtaa gccgacgtac cgtccgaggc tgctcatgta caccctggtc gccctgatcc
     3481 tcaccttttc tgcccttgtg ctggtaggca ttccggcggg gaaggacaag ccaagaggaa
     3541 ggatctccac cacgtcgtct ccctccgtca tctatctgtc tcagctggaa cgtgatgtaa
     3601 tcccagcgga gggtttcacc gtacccgcga aatggggaac cggtgtgagc gagctggtga
     3661 actcccgcgc catcgattcg tcctccttct tctcttcgat gatgggggc
//
LOCUS       DATGDI010000228         5749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_229030, whole genome shotgun sequence.
ACCESSION   DATGDI010000228 DATGDI010000000
VERSION     DATGDI010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229030"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VKO45_08520"
     CDS             complement(<1..328)
                     /locus_tag="VKO45_08520"
                     /inference="COORDINATES: protein motif:HMM:NF021397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2098 domain-containing protein"
                     /protein_id="HMA05952.1"
                     /translation="MAPASRMRGSISAMVSAGSLARCAPRKNFFAPSPISTCMMGEKG
                     GYPFIGGERDDPLMQAGEVAVGMHVRYPRTGTTGKVVRIEKLDRDTFAEIDSTHLLYR
                     TDQLIPF"
     gene            213..1382
                     /locus_tag="VKO45_08525"
     CDS             213..1382
                     /locus_tag="VKO45_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HMA05953.1"
                     /translation="MQVEIGDGAKKFFLGAHRARDPAETMAEMEPLMREAGAIDLVDC
                     TEKDRHGIPCFAVIRRRTGTAHPQVHVGRGITPEAAQASAMATAVERASAEYRGNPMK
                     LALYEEIGIRHAVDPVDLILARPLEAGERIHWSPGYDLLEKEEVWVPSNAVFLPYNTL
                     GEAKGLFASDPNGLAAGNTRDEAILSGLLEVLERDSLSRAERDHSMGKRLSVTGPGPV
                     LDLLGLFEKAGIPITLWLLDGITPVPTVAAAAEDPVTRDPAFLVLGAGAHPSPQGAAI
                     QALTEVAQRRAVRIQGGSASGEREGFVQRVGYDRMKRLNREWFAPAPSVDLADLPDLS
                     TPTIGGDIRRVLGELGGKVERVCVCDLTRTKVPVVRVVVPGLEMSAINKERVRRK"
     gene            complement(1748..2722)
                     /locus_tag="VKO45_08530"
     CDS             complement(1748..2722)
                     /locus_tag="VKO45_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HMA05954.1"
                     /translation="MTCGVVTGKIFCRHQLPGHPESPDRLRLAIGGVPRAARRLDPAE
                     ASREDLERVHRREYLAWLRGVATGECYIDFNTYICPDSFEVARFAAGSAARAAEEALD
                     GHSCFALVRPPGHHAGPGRASGYCLLNNVAVAAARALGSVDRVAIVDWDLHHGNGTQE
                     IFYDDDRVLFCSVHQGEGFPGSGVPGETGAGQGHGYTLNAPLESGCGIADYELVFGEV
                     FCPALERFRPDLLLVSAGQDALADDPKGVMRLAPGDYGILTRMLREATDLPLALTLEG
                     GYGPSLGKAIAAIFRGLGGGRPAQETGPPPARSTREMVRILKKVQASR"
     gene            complement(2743..5364)
                     /locus_tag="VKO45_08535"
     CDS             complement(2743..5364)
                     /locus_tag="VKO45_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006487 - protein N-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HMA05955.1"
                     /translation="MAPFPDLLRKYRKYLVWSALVLIGILCLWIRLNPLSHLQTLAGG
                     DPLNLSASDDPLYNFRQVELIIRHFPAYPWFDPMTLYPFGQVIYWGPLFPAIAAAVCM
                     ILGMTTRPDIIFVSQLIPPVMAVAMVPLMFLVGRKMAGWRTGLLAALFIAIVPGQYLQ
                     RSLFGYFDHHIAEVLFSTLFCLGYISILVYTKRNPVDLGKRETLKIPAILSILTAGAY
                     LLGLFTMPTMILFALIVTAFTVFQFVYDFFRRRPSDYLLLANVIIFGVAILGLLAFGI
                     KHPGLDLSRYTLGHIAAYLMIIVGTGILAGLARFMKERPRYQYPLVLAAIGILVAIFL
                     VGLVPDIYNVLIAGLFSFFGTQAVTLTVQEARGWDPLLAWATFQYGLILMGLGMVAVL
                     YRNWREEHPDQIFVLIWALIILYSTWQHVRYEYYLAVNIALLSAFAVGTGLDRGWGPA
                     RSFIEKRILPVAAAETPVKPKEERPPKRSKRGVKEKAPGKTTGKAPGKTTGNPWIVAL
                     AAVAVLAIFFTYASVQYDGLYSQGTLANGDWKESLEWMGNNTPNPGVDYSGIFDQSTF
                     TYPAGSYGVMSWWDYGHQITFIAKRIPNANPFQAGVAGPAGAAAFFMTRSEEYADSVA
                     DQLGTRYVITDIEMDSPKFWAMATWFNSSVNDTPYNPAYYVQSQTAGQYELVKLNNQS
                     YYETMVSRLHNFDGSMATPSSVYYIEYQLPSVTGLDWPLITNAQILSAGDAKNRTVQF
                     NANAPLGKLANTVSQALFLPVDTVPALRHYRLVHESPSGVYSNASAPDIKYVKVFEYV
                     KGARIRGQGVIALDLVTEAGRRFTYRQTSQDGGFVVPYSTQGNPSGVRAVGKYRIEGT
                     GLSFDVPESAVIQGSAI"
BASE COUNT         1078 a         1703 c         2007 g          961 t
ORIGIN      
        1 cgaaggggat gagctggtcg gtgcggtaga ggaggtgggt gctgtcgatc tcggcaaagg
       61 tgtcccggtc gagcttctcg atcctgacca ccttcccggt ggtcccggtc cggggatacc
      121 tgacatgcat cccgaccgcg acctctcctg cttgcatgag aggatcatcc ctttcgccac
      181 ctataaaagg atagcctccc ttctctccca tcatgcaggt ggagatcggg gacggggcga
      241 agaagttctt cctcggggcg caccgggcga gggacccggc agagaccatg gcggagatgg
      301 agcccctcat gcgggaggca ggtgccatcg acctcgtgga ctgcaccgag aaggaccggc
      361 acgggatccc ctgcttcgcc gtcatcaggc gcaggacggg gacggcgcat ccccaggtcc
      421 acgtggggag ggggatcacc ccggaggctg cccaggcctc ggccatggcc acggccgtcg
      481 agcgggcctc ggccgagtac cgggggaacc ccatgaagct cgccctctac gaggagatcg
      541 ggatccgcca tgctgtcgat cccgtggacc tcatcctcgc ccggcccctg gaggccgggg
      601 agaggatcca ctggtccccc ggctacgacc tcctcgaaaa ggaggaggtc tgggtcccga
      661 gcaacgcggt gttcctcccc tacaacacgc tgggggaggc aaagggcctc ttcgcctcag
      721 acccgaacgg cctcgcggcg gggaacaccc gggacgaggc gatcctctcg gggctcctcg
      781 aggtcctgga gcgggactcc ctctcccggg ccgagcggga ccactccatg gggaagaggc
      841 tctcggtgac cgggccgggc ccggtcctgg atctcctcgg gctcttcgag aaggccggga
      901 tccccatcac cctctggctc ctcgacggca tcaccccggt tccgacggtg gccgccgcgg
      961 ccgaagaccc ggtgacccgg gaccccgcct tcctcgtcct cggggccggt gcccaccctt
     1021 cgccccaggg ggcggccatc caggccctca ccgaggttgc ccagcggagg gcggtgcgga
     1081 tccagggggg gtctgcctcg ggggagcggg agggcttcgt ccagcgggtc gggtacgacc
     1141 ggatgaagcg cctgaaccgg gaatggttcg cgccggcgcc ctccgtggac ctcgccgacc
     1201 tccccgacct ctcgaccccg accatcgggg gggacatccg gcgggtcctc ggggagctcg
     1261 gggggaaggt ggagagggtc tgcgtctgcg acctcacccg gacgaaggtg cccgtggtcc
     1321 gggtcgtcgt cccggggctc gagatgtccg ccatcaacaa ggaaagggtg cgccggaaat
     1381 aaccggcggg atgcggggtc ccgggtcagg tacagaccgg ggcaatcgga gaggatcagg
     1441 gaagagcatc tcctcaccgg aaagaagggc caatcccgga caccagtttc aggctctaaa
     1501 agatccggga ctgaagacgc cgttgaccag ggtggggaag cctcggggga tgagaggtga
     1561 tagcgtgcag agaagtgatc tcactcgtac cgggtccacc atgttctggc aacatgtaca
     1621 tgctcgccgt aaggtattat aaaacttttg atataattct acgctgatta cgcaaaataa
     1681 ttcagaaaat ttttacgcaa taatggagga ttcccgaaag aggccggaat tgcctgtccc
     1741 ggagagatca ccgggaggcc tgcacctttt tcaggatcct gaccatctcc cgcgtggatc
     1801 gggccggcgg cggtcccgtt tcctgggccg gccgaccccc accgaggccc cggaagatgg
     1861 cggcgatggc ctttccgagg gaggggccgt acccgccctc gagggtgagg gccaggggga
     1921 gatcggtggc ctcccggagc atccgggtga ggatgccgta atcacccggg gcgagcctca
     1981 tcaccccctt gggatcatcg gcgagggcat cctggccggc cgagacgagg aggaggtccg
     2041 gccggaaccg ttcgagggca gggcagaaga cctcgccaaa gacgagctcg taatccgcga
     2101 tgccgcagcc cgattccagg ggggcgttca gggtataccc gtgcccctgg cccgccccgg
     2161 tctcccccgg gacgccgctc cccgggaacc cctccccctg gtggacggag cagaagagca
     2221 cccggtcgtc gtcatagaag atctcctggg tcccgttccc gtgatggagg tcccagtcca
     2281 cgatggccac ccggtccacg gacccgaggg cccgggcggc cgcgacggcc acgttgttca
     2341 ggagacagta cccgctcgcc cggcccggcc cggcatggtg gccggggggc cgcacgaggg
     2401 cgaagcagct gtgcccgtcg agggcctcct ccgcggcccg ggcggccgac ccggccgcga
     2461 accgggcaac ctcgaaggag tcggggcaga tgtaggtgtt gaagtcgatg tagcattcac
     2521 cggtcgcgac cccccggagc caggcgagat actcgcgccg gtggacccgc tccaggtcct
     2581 cccgggatgc ctcagccggg tcgagcctcc gggcagccct cggcaccccg cctatggcaa
     2641 gccggagccg gtcggggctc tccgggtgcc ccggcagctg atgccggcag aagatctttc
     2701 ccgtgacgac accgcaggtc atgtccgtgg aggggaatgg gatcagatgg ccgagccctg
     2761 tatcaccgcc gattccggaa cgtcgaagga gaggccggtt ccctcgatcc tgtacttccc
     2821 gacggcccgg accccggagg ggttcccctg ggtcgagtag gggaccacga atccgccgtc
     2881 ctggctcgtc tgccggtagg tgaaccgcct tcccgcttcc gtgacgaggt ccagggcgat
     2941 gactccctgg ccccggatcc gggcaccctt cacgtactcg aagaccttca cgtacttgat
     3001 gtcgggggcg gaggcattgg aatagactcc cgagggggat tcatggacca ggcggtagtg
     3061 tcgcaacgcg ggcacggtgt ccaccgggag gaagagcgcc tgcgaaacgg tgttcgcgag
     3121 cttcccaagg ggggcgttcg cgttgaactg gaccgtcctg ttcttcgcgt ccccggcact
     3181 gaggatctgg gcgttcgtga tgagaggcca gtcgagtccg gtgaccgagg ggagctggta
     3241 ctcgatgtag tagaccgagg acggggtcgc catggacccg tcgaagttgt ggagccggga
     3301 gaccatcgtc tcgtagtagg actggttgtt cagcttcacg agctcgtact gcccggccgt
     3361 ctggctctgc acatagtagg ccgggttgta tggcgtgtcg ttcaccgaac tgttgaacca
     3421 ggtagccatg gcccagaact tggggctgtc catctcgata tcggtgatga cataccgggt
     3481 cccgagctgg tcagcaacgg aatcggcata ctcctcggac cgtgtcatga aaaaggcggc
     3541 agcgccggcc gggcccgcga ccccggcctg gaagggattt gcattgggga tgcgttttgc
     3601 gatgaaggtg atctggtggc cgtagtccca ccaggacatc accccgtagg agccggccgg
     3661 gtaggtgaag gtcgactggt cgaagatgcc ggagtagtct acacccggat tgggggtgtt
     3721 atttcccatc cactcgaggg actccttcca gtcaccgttc gcgagcgttc cctgggagta
     3781 gaggccgtcg tactggaccg aggcgtaggt gaagaaaatg gcaaggacgg ccacggccgc
     3841 aagggcgacg atccaggggt tcccggttgt ctttccgggg gcctttccgg ttgtctttcc
     3901 gggtgccttc tccttcacac cccgcttcga tctcttcggg ggcctctcct ccttcggttt
     3961 caccggggtt tcggccgctg ctaccgggag gatccgtttc tctatgaagc tccgggcagg
     4021 tccccaaccg cgatcgagcc ccgtcccgac cgcgaaggcc gagaggaggg cgatgttcac
     4081 cgcgaggtag tactcgtacc tgacatgctg ccaggtggag tagaggataa tgagggccca
     4141 gatgaggaca aagatctggt cggggtgctc ctcccgccag ttgcggtaga ggacggccac
     4201 catgccgagg cccatgagga tgaggccgta ctggaacgtg gcccaggcga ggaggggatc
     4261 ccagcctctc gcctcctgca cggtcagggt gactgcctgg gttccgaaga agctgaagag
     4321 accggcgatg aggacgttgt agatatccgg gacaaggcct acgaggaaga tcgccacaag
     4381 gatcccgatt gcggccagga ccagggggta ctgatacctg ggcctctcct tcatgaaccg
     4441 ggcgagaccg gcgaggatac cggtgccgac gatgatcatg aggtaggcgg cgatgtgacc
     4501 cagggtatag cgggagagat cgagccccgg gtgcttgatc ccgaaggcga ggaggccgag
     4561 gattgccacg ccgaagatga tgacattcgc gagaaggagg taatcgctcg gcctccgccg
     4621 gaagaagtca tagacgaact ggaacacggt gaaagcggtc acgatgaggg cgaagaggat
     4681 catggtgggc atggtgaaga gcccgaggag gtacgcaccg gcggtcagga tggagaggat
     4741 ggcagggatc ttcagggtct cccttttccc gaggtccacc ggattccgct tcgtgtacac
     4801 gaggatcgag atgtagccca ggcagaagag ggtggagaag aggacctcgg cgatgtggtg
     4861 gtcgaagtac ccgaagaggg agcgctggag gtactgcccc gggacgatgg cgatgaagag
     4921 cgcggcgagg aggccggtcc tccagcccgc cattttcctc ccgacgagga acatgagggg
     4981 gaccatggcg acggccatga cgggcgggat gagctgggag acgaagatga tgtcaggccg
     5041 ggtggtcatc cccaggatca tgcagaccgc ggcggcgatg gcggggaaga gcgggcccca
     5101 gtagatgacc tggccaaaag ggtagagggt catggggtcg aaccaggggt aggccgggaa
     5161 gtgcctgatg atcagctcca cctgccggaa gttgtagagg ggatcgtcgc tcgccgacag
     5221 gttcagggga tccccgcccg caagggtctg gagatgggag agagggttca accggatcca
     5281 gaggcagagg atcccgatga gcacgagggc agaccagacg aggtatttcc ggtacttccg
     5341 taaaaggtcg gggaacgggg ccatgctatc ctccctctct ctttaggcgt cacctgaatt
     5401 aaagggttct taccgaaaat tacatggagt atcctccccc catccttaca ggggaaaccc
     5461 ggctgaaggg aacccgctac cgtgaaagag aaatactatg tggtatccac cggtccgggc
     5521 aatgtatgag cggttccttc gccgtgatgg gcgaataccg aggagaatac gggaaatatc
     5581 ctgtttctct cctgtatcca gggctttggg ggaggtatga ataaaagggc cggggtgggc
     5641 atctaagatg gtgaccttgg gaatctgcca tgtgaaaacc gagtttggca cccggaaacc
     5701 tgagtggcgg cacgacgtat aaagttgggg ggggtgagcc agatgtgcg
//
LOCUS       DATGDI010000229         5412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_287134, whole genome shotgun sequence.
ACCESSION   DATGDI010000229 DATGDI010000000
VERSION     DATGDI010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287134"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VKO45_08540"
     CDS             complement(<1..677)
                     /locus_tag="VKO45_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMA05956.1"
                     /translation="MENEPLLMIPGPVPVPERVRMAMLRQAINHRGPEFGAIYAESVR
                     VLKGLFRTGNELFILSGSGTAAMEAAIGNFGRDRRIVSLVNGKFGERMGKIAERYGDV
                     KLIQSDWGTPLDLEGLAKALGEGADLVTLVHNETSSGIRNPAAEVGRLARKHDALFVM
                     DGITSIGGDVVDMDGWGVDVAFVGSQKCLAAPPGLSAISVSKRAWERLSERRPYYLDL
                     AAYRKSAA"
     gene            725..1126
                     /gene="purE"
                     /locus_tag="VKO45_08545"
     CDS             725..1126
                     /gene="purE"
                     /locus_tag="VKO45_08545"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HMA05957.1"
                     /translation="MPPLPRADVAIISGSTSDAHIVEKVVKVLEEHHVPYEHHVISAH
                     RNPDELDAFLKKSRAKIFIAVAGMSAALPGVIASKTDKPVIGVPVSGKLGGFDSLLSI
                     AQMPKGVPVACVAVDGGENAALLAIRILKLK"
     gene            complement(1129..2415)
                     /gene="thiC"
                     /locus_tag="VKO45_08550"
     CDS             complement(1129..2415)
                     /gene="thiC"
                     /locus_tag="VKO45_08550"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HMA05958.1"
                     /translation="MCVMSSLPALCRTGVPDPVLSAAGEEGIPPEQLARDIARGWAVI
                     PSNPRRRHRMAAIGAGCRVKVNVNVGTSGIRCDPELELEKAKAALTNGADTLMDLSTA
                     GDLPAIRERILSLDAPVGTVPVYEAVRRAGSAVDLDGDLLFRVIREHCRQGVDFLTLH
                     TGVNLAALDALHADPRIMGVVSRGGVFHIARMVETGEENPLFAEFDYLMEILLEEDVV
                     ASLGDGMRPGCHHDAGKLAKTTEYLTLGRQAKRALDLGVQRMIEGPGHIPLAQVSHNI
                     RMIKELTGNAPLYLLGPLVTDIATGYDHVAAAVGGAVACMDGADFLCMVSPSEHLALP
                     SVEEIVEGTRVCRIAAHIGDTVRCTGKDGSERELAMALARRDLDWERQFATALYGDVA
                     RRIHERDGDLETCSMCGDLCAVKMVKDLLSGGKRKE"
     gene            2442..3044
                     /locus_tag="VKO45_08555"
     CDS             2442..3044
                     /locus_tag="VKO45_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA05959.1"
                     /translation="MPVTWIPGSGFWANSYLVGNVLVDCGVLPMQVAPYRDRIGEIVL
                     THCHYDHTAHLTEIRAMTGGRVSVHRADAESLGNDLLSSAMLFGEKAAPVTVDRFLAD
                     GDRVGDLTVIHTPGHTPGSVCLYHPTEEFLISGDTVFTHGSFGRYDLPGGSLPAVRES
                     LRRLSGYSIRGIYPGHGEPVREGGSRHIAAALKAVGDLHG"
     gene            3037..3474
                     /locus_tag="VKO45_08560"
     CDS             3037..3474
                     /locus_tag="VKO45_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF123 domain-containing protein"
                     /protein_id="HMA05960.1"
                     /translation="MDKGIYALVFRNRSCRLTIGSLGEVRFRRGWHVYVGSARGPGGL
                     ARVRRHRRLSIARDRAPKWHVDHLLVSPFFSLHAIVCGPTGKDLECSLAGALGGGCVP
                     SFGSGDCACEGHLFYRPKDPGKEIMEAMAGLGLKPVSTTLIKG"
     gene            3501..4052
                     /locus_tag="VKO45_08565"
     CDS             3501..4052
                     /locus_tag="VKO45_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HMA05961.1"
                     /translation="MQVVGISGSMRRDGNTARLVRLILDQVKDAGIKAQYITLADKVI
                     KPCLGCESCKEERTCAVKNDDWHKIIDRVLACEVLILGSPTYYYDVNGQTKNFIDRTY
                     SLYHDRQLAGRRAVGVAVCADKGGKRALQTMEGFFNSHEFSYLGGVLGKGYLAGEVLE
                     NRAAVQAAERIGKKIVSLLRPQD"
     gene            complement(4254..5342)
                     /gene="cofH"
                     /locus_tag="VKO45_08570"
     CDS             complement(4254..5342)
                     /gene="cofH"
                     /locus_tag="VKO45_08570"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HMA05962.1"
                     /translation="MVAGIRVLLRDVAGGHRLTEAEALRLMGATGRDVWAVAGCADRL
                     REERVGEAVTYVRNQNLNVTNICVNSCGFCGFSRKRGDPDAYFLDRAAIQAKAARARE
                     RKATEICTVSGLHPDFTADSYVDILTAIREAAPGVHLHASNPMEVAYGAARSGISTAE
                     MAGRLKAAGLGSMCGTAAEILVDRVRSVICPGKIDTASWERIIREVHGLGIPTTATIM
                     YGHCETVADRARHLGILRGIQDDTGGFTEFVPLSFIHMETPLFRKGLARAGATGREDL
                     LMIAVSRLFLDNIPHIQSSWVKLGRKLAQLGLVAGADDLGGTMYEESISKGAGAKDTE
                     YLDPAEMERMAEDLGRPLRQRTTLYTLL"
BASE COUNT          856 a         1877 c         1768 g          911 t
ORIGIN      
        1 gcggcgctct tccggtaggc ggccaggtcc aggtagtacg gcctcctctc cgagagacgc
       61 tcccaggccc tcttcgagac cgagatggcc gagaggcccg gcggggccgc caggcacttc
      121 tgggagccga cgaaggccac atccaccccc cagccgtcca tgtccaccac gtcgccgccg
      181 atggaggtga tgccgtccat gacaaagagg gcgtcgtgtt tccgggcgag cctccccacc
      241 tcggccgcgg ggttcctgat ccccgaggag gtctcgttgt ggaccagggt cacgaggtcg
      301 gccccctccc cgagggcctt cgcgaggccc tccaggtcga ggggggtccc ccagtcggac
      361 tggatcagct tcacgtcgcc gtatcgctcc gcgatcttcc ccatccgctc cccgaacttc
      421 ccgttcacca gggagacgat cctccggtcc ctcccgaagt tcccgatggc ggcctccatg
      481 gcggccgtcc cggaccccga gaggatgaag agctcatttc ctgtgcggaa gagccccttc
      541 agcacccgga cggactctgc atagatggcc ccgaactcgg gcccccggtg gttgatggcc
      601 tgccggagca tggccatccg cacccgctcg gggacgggaa ccgggcccgg aatcatgagg
      661 aggggttcat tctccatggc gatcagccat aatatatgta tggcgaggaa taagtaagct
      721 ctggatgccc cccctgcccc gtgcggatgt cgccatcatc tcgggatcga cctccgatgc
      781 gcacatcgtg gagaaggtcg tgaaggtcct cgaggagcac catgtgccgt acgagcacca
      841 cgtgatctcg gcccaccgca accccgatga actcgatgcg tttctcaaaa agagcagggc
      901 gaagatcttc atcgccgtcg ccgggatgtc cgccgccctt ccgggggtca tcgcctcgaa
      961 gaccgataag ccggtcatcg gggtccccgt gagcgggaag ctcggggggt tcgactccct
     1021 cctctccatc gcccagatgc cgaagggagt gccggtggcc tgcgtggcgg ttgacggcgg
     1081 ggagaacgcg gccctcctcg cgatccggat cctgaagctg aaataatcct attccttcct
     1141 ctttccgccc gaaagcaggt ccttcaccat cttcacggcg cagaggtccc cgcacatgga
     1201 gcaggtctcg agatccccgt ccctctcgtg gatccgcctc gccacatcgc cgtagagggc
     1261 ggtcgcgaac tgccgttccc agtcgaggtc ccgcctcgcg agggccatgg cgagctcccg
     1321 ctccgacccg tccttcccgg tgcacctcac ggtgtcgccg atgtgtgccg cgatcctgca
     1381 cacccgggtc ccctcgacta tctcttcgac actgggaagg gcgaggtgct cgctcgggga
     1441 gaccatgcag aggaagtcgg ccccgtccat gcaggccacc gcgccgccca cggccgctgc
     1501 cacgtggtca tagcccgtgg cgatgtcggt cacgagcggg ccgaggaggt agaggggggc
     1561 gttcccggtg agctccttga tcatccggat gttgtggctc acctgggcga gggggatgtg
     1621 ccccggtccc tcgatcatcc tctggacccc gagatcgagg gcccgtttcg cctggcggcc
     1681 cagggtgagg tactcggtgg tcttcgcgag tttccccgcg tcatggtggc atccgggccg
     1741 catcccgtcg cccaggctcg ccaccacatc ctcctcgagg aggatctcca tcaggtagtc
     1801 gaactcggcg aagagggggt tctcctcgcc ggtctccacc atcctcgcga tgtggaaaac
     1861 cccgccccgg gacacgactc ccatgatccg ggggtcggca tggagggcgt cgagggccgc
     1921 gaggttcacg cccgtgtgga gggtgaggaa gtccaccccc tgccggcagt gctcccggat
     1981 cacccggaag aggaggtccc cgtcaaggtc caccgcgctc cccgcccgtc tcaccgcctc
     2041 gtagaccggg accgtcccca cgggagcatc gagggagagg atccgctccc tgatggccgg
     2101 gaggtccccg gccgtcgaga gatccatgag ggtgtcggcc ccgttggtaa gggccgcctt
     2161 ggccttctcg agctcgagct ccggatcgca gcggatgccc gaggtgccga cgttcacgtt
     2221 caccttcacc cggcagcctg ccccgatggc ggccatccgg tgtctcctcc gggggtttga
     2281 agggatgaca gcccatcccc tcgcgatatc ccgggcgagc tgttcgggcg ggataccctc
     2341 ctctccagca gcagaaagga cggggtccgg cacgccggtc ctgcacaggg ccgggaggga
     2401 agacataaca cacatgtagt gccccgggct tattatctta catgcctgtc acgtggatcc
     2461 cgggcagcgg gttctgggca aactcctacc tcgtgggaaa cgtcctcgtg gactgcgggg
     2521 tcctccccat gcaggtcgcc ccctaccgtg acaggatcgg ggagatcgtc cttacccact
     2581 gccactatga ccacaccgcc cacctcaccg agataagggc catgaccggc ggccgggtct
     2641 cggtccaccg ggcggacgcc gagagcctcg ggaacgacct cctctcctct gccatgctct
     2701 tcggcgaaaa ggcggccccg gtcaccgtcg atcggttcct ggcggacggg gaccgggtgg
     2761 gggatctcac ggtcatccat accccgggcc acacccccgg gtccgtctgc ctgtaccacc
     2821 cgacggagga gttcctcatc tcaggggaca ccgtcttcac ccacgggtcc ttcgggaggt
     2881 acgacctccc gggggggagc ctcccggccg tcagggagtc cctgcggagg ctttccggct
     2941 actccatccg ggggatctac cccggccacg gggaaccggt ccgggagggc gggagccggc
     3001 acatcgccgc ggcgctgaag gccgtcgggg acctccatgg ataagggtat ctatgccctc
     3061 gtcttccgga accgttcctg ccggctcacc atcgggagtc tcggggaggt ccgcttccgg
     3121 agagggtggc acgtctatgt cgggtccgcg agggggcccg gcgggctcgc ccgggtgaga
     3181 cggcaccgcc ggctctccat cgcgcgggac cgggccccga agtggcacgt ggaccacctc
     3241 ctggtctccc ccttcttctc tctccatgcc atcgtctgcg ggcccacggg gaaggacctg
     3301 gagtgttccc ttgccggggc gctcgggggc gggtgcgtcc cgtccttcgg gtcgggggac
     3361 tgcgcctgcg agggacacct cttctaccgg ccgaaggacc ccgggaagga gatcatggag
     3421 gccatggcag ggctcggtct caaacccgtg agcacaaccc tcattaaggg gtaagttcag
     3481 gtggtacgat gggagtgcct atgcaggtag tgggaatctc cggctccatg cgcagggacg
     3541 ggaataccgc ccggctcgtc cggctcatcc tcgaccaggt gaaggatgcc gggatcaagg
     3601 cccagtacat cacccttgcg gacaaagtga tcaagccctg cctcgggtgc gagagctgca
     3661 aagaggagcg aacctgcgcc gtgaaaaacg acgactggca caagatcatc gacagggttc
     3721 tcgcgtgcga ggtcctgatc ctggggtcac ccacctatta ctacgacgta aacgggcaga
     3781 cgaagaactt catcgaccgg acctactccc tgtaccacga caggcagctc gccggaagga
     3841 gggcggtcgg tgtggccgtt tgcgccgaca agggcgggaa gagggccctc cagaccatgg
     3901 aggggttctt caactcccac gagttctcgt acctcggcgg ggtcctcggg aagggctacc
     3961 tcgcggggga ggtcctcgag aacagggccg cggtccaggc cgccgagcgg atcggcaaaa
     4021 agatcgtcag tctcctgcga ccccaggact agatcccggg tcccgccgga gccgggatcg
     4081 tccagggggg tgggggcccc tctggaccgg gtcccgcagg agccccggtt ccgaaggaac
     4141 ctgggctgtc ccggggggtt tggggcccct cgggaccaag ccgggatcgt ccaggggggt
     4201 gggggcccct ctggactggg caggggcccc tctggacagg atcccaggca ccttcagaga
     4261 agggtgtaga gggtggtccg ctgccgcagg ggccgcccga ggtcctctgc catccgctcc
     4321 atctccgccg ggtccaggta ctcggtatcc tttgccccgg cacccttcga gatggactcc
     4381 tcgtacatgg tcccccccag gtcgtcggcc cccgcgacga ggccgagctg ggcgagcttc
     4441 ctcccgagct tcacccagga cgactggatg tgggggatgt tatccaggaa gaggcgcgat
     4501 acggcgatca tgaggagatc ctcccttccc gtggctcccg ccctcgcgag gcccttccgg
     4561 aaaagggggg tctccatgtg gatgaaggag aggggcacga actcggtgaa tcccccggtg
     4621 tcatcctgga taccccggag gataccgagg tgacgggccc ggtccgccac ggtctcgcaa
     4681 tggccgtaca tgatggtcgc cgtggtcggg atgccgaggc cgtggacctc ccggatgatc
     4741 cgctcccagc tcgccgtgtc gatcttcccc gggcagatca ccgaccggac ccggtcgacc
     4801 aggatctcgg ccgccgtccc gcacatggac ccgaggcccg ctgccttcag cctgcctgcc
     4861 atctcggcgg tggagatccc gctccgcgca gccccgtagg caacctccat aggatttgag
     4921 gcatgaaggt ggacgcccgg cgccgcctcc cggatcgcgg tgaggatatc gacgtaagag
     4981 tcggcggtga agtccgggtg gagaccgctc accgtgcaga tctcggtcgc cttccgttcc
     5041 cgggcccggg cggccttggc ctggatcgcc gcccggtcga ggaagtaggc atccgggtcc
     5101 ccccgcttcc gggagaaacc gcagaacccg caggagttca cgcagatgtt cgtcacgttc
     5161 aggttctggt tccggacgta ggtgaccgcc tcgccgaccc gctcctcccg cagccggtcg
     5221 gcgcaccccg ccaccgccca gacgtcccgg ccggtcgccc ccatgagccg gagggcctcc
     5281 gcctccgtga gccggtggcc gcctgccacg tccctcagga gaacccggat cccggccacg
     5341 atcctcaccg cttccctttc ttcttctcct tctccttccc cttcttctcg ttgccctccg
     5401 cccgggagac gt
//
LOCUS       DATGDI010000230        17026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_432861, whole genome shotgun sequence.
ACCESSION   DATGDI010000230 DATGDI010000000
VERSION     DATGDI010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 17026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17026
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432861"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VKO45_08575"
     CDS             <1..543
                     /locus_tag="VKO45_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HMA05963.1"
                     /translation="RGRTPEEDERLAQELRSDEKERAEHLMLVDLARNDVGRVSRFGS
                     VRVEDFMAVERFSHVQHLVSTVHGTLSDHLTPFDALKSCFPAGTVSGAPKIRAMEIIE
                     ELEPERRGLYAGAVGYAGFDRTLEFAITIRTIVARGRRAEVQVGAGIVADSDPGREWE
                     ETESKGMALIRAIELVEGSG"
     gene            540..1115
                     /locus_tag="VKO45_08580"
     CDS             540..1115
                     /locus_tag="VKO45_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HMA05964.1"
                     /translation="MRVLIIDCYDSFTYNLTQLVGSAGAIPEVILCDAPQDALERHSW
                     DRIILSPGPGRPEDSGLCLTALSTLSHDIPTLGVCLGHQAVCTAFGGRVVRGQRPVHG
                     KTSPVSHDGKGIFTGLGNPFTATRYHSLVAERASLPSDLEVSAECPEDGCVMGVRHRR
                     YPIDGVQFHPESILTGEGPRIIGNFLSGEGA"
     gene            1112..2128
                     /gene="trpD"
                     /locus_tag="VKO45_08585"
     CDS             1112..2128
                     /gene="trpD"
                     /locus_tag="VKO45_08585"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HMA05965.1"
                     /translation="MTPKEAILALTSGRDLNREEAASVMRAVMAGDATPAQIGAILAS
                     LSMKGARSQELAGFAGAMREFAVPFPHSLAGPFVDTCGTGGDGSGTFNVSTAAAFVVS
                     GAGLPVVKHGNRSVTSRCGSADVLTTLGARIEIPPDRMAGVLRATGIAFLYAPLYHPA
                     MRRVMGVRQELGVRTVFNLLGPLTNPAGARIQLLGVYRPDAVLTMAEVLRLLGAERAM
                     VVHGEGIDEITTAGPTRVAEIDGDGIREYTLHPGDFGIPVSSIEDLRGGDAAENARIL
                     REVLSGEMGAARDAVLMNAGAAIYLGGGAQSIAGGLSRAEESLDSGQALEKLGAFIDA
                     TVGA"
     gene            2125..2892
                     /locus_tag="VKO45_08590"
     CDS             2125..2892
                     /locus_tag="VKO45_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HMA05966.1"
                     /translation="MTVILDEILEATEERVRALEGRTFSLPPAAGPHRSLNRAVCRPP
                     GRNAIIAEVKFASPSRGRIRAPTSPDGIARDLVRGGCCALSVLTEPGFFAGSTASLTR
                     VRGAVGVPVLRKDFIIHPVQLDETRALGADAVLLIAALLGDRLSGFVDRALALGIEPL
                     VEVHTREEAEAAIRTRSNLIGVNNRDLGSMEIDLATTIALGPGIRKAGKRVVSESGIR
                     SPDDIRTLRDSCDAFLVGSSLMASEDPGKTLEALVCA"
     gene            2883..3518
                     /locus_tag="VKO45_08595"
     CDS             2883..3518
                     /locus_tag="VKO45_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HMA05967.1"
                     /translation="MRVKISGPVPLPGNHRETRVRVKICGITRAEDAIHAEKAGADAI
                     GVILFSDSPRCISLETAGEIFRSVGPFITTVAVTHTKTRKDLEEIITLHPGAVQISHP
                     FTFPAPPPVRVLRVVKPGDPLPGDADALVVDQSMGTGRHYDPGFAREMVRRSRVPVTL
                     AGGLTPGNVADAIRAIRPYAVDVASGVEISPGIKDPARVTAFIRAAQGVLP"
     gene            3515..4687
                     /gene="trpB"
                     /locus_tag="VKO45_08600"
     CDS             3515..4687
                     /gene="trpB"
                     /locus_tag="VKO45_08600"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HMA05968.1"
                     /translation="MRKGRFGAFGGRFVSEALMGALAELEEAFERFRKDPAFLSSLNS
                     YLTEYAGRPTPLTFCANLSRDMGCRVYLKREDLLHGGAHKLNNTLGQALLARHMGKKR
                     LIAETGAGQHGVATAIAGAVLGLPVEVYMGEVDIARQRPNVFRMELMGAKVIPVSSGT
                     RTLKDAVNEALRDWAASAKETHYLLGSVVGPHPFPLMVRDFQSVIGREARAQVLEKEG
                     RLPDAVIACVGGGSNAIGIFHPFLSDRVRLIGVEAGGEGLGTGRHGASLCAGSPGILH
                     GALSYLLQDGDGQVLSTHSVAAGLDYPGVGPEHSMLKNSGKAEYIAVTDREALDAFTL
                     LSRREGIIPALESAHALAGLVNLKEEFGRDDIVVVNLSGRGDKDLAEVARIAGERP"
     gene            4684..5478
                     /gene="trpA"
                     /locus_tag="VKO45_08605"
     CDS             4684..5478
                     /gene="trpA"
                     /locus_tag="VKO45_08605"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HMA05969.1"
                     /translation="MTRIGELFASLDRPAFIAYLVTGDPDPGGFATAVRAVLDGGADI
                     LELGMPFSDPVADGPTLQRAHIRALEGGMTTDRYFEAVREIRRDSEIPLILMVYCNTV
                     HRRGVDRFYREARDAGADGVLVVDMPPEEGERALAAAKMNDLDQIFLIAPSTSRERLD
                     LIAKSGSGFLYLASLLGVTGARERLSDEASSLIGRVRPRTRLPLAVGFGISRPEHAGA
                     LAKAGADGIIVGSAIVKIVEERHRDGARMAGEIRDFVAGMRRALEG"
     gene            complement(5486..6013)
                     /locus_tag="VKO45_08610"
     CDS             complement(5486..6013)
                     /locus_tag="VKO45_08610"
                     /inference="COORDINATES: protein motif:HMM:NF015239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HMA05970.1"
                     /translation="MIKNLFVTGAPGCGKTTLVRRVVESLGDLPMAGFYTEEIREKGE
                     RVGFALVSLDGKRGILSHVTVKGPLRIGKYGVDTGGFESFLGELRLGDPGTRVVVIDE
                     IGKMECLSERFGNLMREILGSPVPLLATIALRGTPAIERVKKRPDVRVITMTREDRDM
                     KFGEVLGEIRALLGT"
     gene            complement(6010..8292)
                     /locus_tag="VKO45_08615"
     CDS             complement(6010..8292)
                     /locus_tag="VKO45_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMA05971.1"
                     /translation="MPVPDLIASLEKDPRQARRVAHVRVIPAQEANYATPVRPLPPVL
                     DRYLDRKGIRLFTHQVEALEAMRDGENLIITTPTASGKTLAFNLPIFEALHDRPEATA
                     LYLYPTKALANDQLKAIQGLEEGTGIATAGAIYDGDTPSSRRPLIRERSRIILSNPYE
                     LHQVLPWHHRWERFFRNLSFVVIDEAHRYRGVFGSHMAYLIRRLRRIARHYGADPQFV
                     LSTATIANPLEFGRNLTGHDYHHVHADGSPRGRKHFVLYNPFPDGVNGKSVHRETEEL
                     LLSCLSHELQTLCFTVSRRMAELIAMWAHEDLARKGRDPGQVTAYRAGYLPEERRAIE
                     DRLKSGRLRGLTSTNALEVGIDIGGLDAVLISGFPGTIISTWQQAGRAGRRGNDSAAF
                     LIAFQDLLDQYFMRHPDTFFGSPHEHAIVDLANPYIVSGHLLCAASELPVNPDRDRPL
                     WPGELDPPIRSLTEAHLLRPTPHGAVYCGRGRAAEAVKLDSISSETFRMTCNGTLLET
                     LDRAQAYREAHEGAVVLHRGETYLVREMDLDTRRIRVERADVDYYTDALKTVDLRVVR
                     EISRREGEGFTLCLGDVEVTEQYTGYRIMKYDALVGFEPLSLPPLKFGTRAFWFTLSP
                     GLAASVVAGGFDLAGSLHGAEHAVIGIAPFHVLCDRRDLGGVSTPAHPDTDGPAIFVY
                     DGYEGGIGLAEKAFELMDRIASMACEAVRECRCEAGCPACIYSPKCGNDNTPLDKGGT
                     VQLLGALGGFHGRTEGVREG"
     gene            complement(8279..9358)
                     /locus_tag="VKO45_08620"
     CDS             complement(8279..9358)
                     /locus_tag="VKO45_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HMA05972.1"
                     /translation="MLQTLHRVSERWRAAVDAVPGYEVVEDGKIFRTSFLDSYVFSSE
                     HGRALELLGSMISLHEGRPVRQVLPGREVETGFGPAWCIEARTEVPDLHPDRNRVTEC
                     LLGELRLLEGIGAVTEKRLRGRGCRSILDLRDHRRFGPGAREFLAMLDRAETVPLAGW
                     ISRWFPRSHPLSLAPCGLHRRDELVFLDIETLGIFSRPIVLIGIGRLAGSTLATSQYL
                     VREISEEPAALNAALEDLTGEGAAYVSFNGKSFDIPYIGERAAYYGMPVRLDRPHFDL
                     LHFSRRRWGRELPDCRLSTLERHVFGISRGVDIPGSLVPEFYEAFLRTGNCGPLVPIV
                     DHNRQDLVSLGYLLSTLLEDLHARS"
     gene            complement(9463..10104)
                     /locus_tag="VKO45_08625"
     CDS             complement(9463..10104)
                     /locus_tag="VKO45_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HMA05973.1"
                     /translation="MRRELEEEIGKLSALAREKDAKTTVISADSLVVAEWVRYKCRYG
                     CKGYGKHLSCPPYAPSPEETRRMVAGYSTGILVRFDGIPGHKHLRPEEIPEDFHPFYR
                     DLILWGHTTVHLLEKTAFYDGFYKAFGFGPYPCIFCEHCVAEEQPGVVDESIRRLCRH
                     MDLVRPSMEAAGMDVFATAKNAGWDLSTIPCKNLEYGKIVHGDIVSVGLVLLE"
     gene            complement(10176..10598)
                     /locus_tag="VKO45_08630"
     CDS             complement(10176..10598)
                     /locus_tag="VKO45_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HMA05974.1"
                     /translation="MKRVVATGTFDIIHPGHIYYLEESRKLGEELHVIVARDANVRHK
                     PRPVIGEAQRLRVVQALRMVDHARLGDPIDMFRPIEEIAPAIITLGFNQHFSEEDLTI
                     ALKERGIGARVVRIGEYSADPCSSTRKIIERILETRGK"
     gene            complement(10595..10975)
                     /locus_tag="VKO45_08635"
     CDS             complement(10595..10975)
                     /locus_tag="VKO45_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HMA05975.1"
                     /translation="MKIRGIARDLLQALLESAREVDREGGGREFVAVLREEDGVIREF
                     YFLPFTSTTQSASLRYDLMPLGLHVAGSAHSHPSGALRPSDADLRFFPALGRYHLILG
                     PPFSETSWRCFTSDGEPCELEVVE"
     gene            complement(11082..12494)
                     /locus_tag="VKO45_08640"
     CDS             complement(11082..12494)
                     /locus_tag="VKO45_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase-like protein"
                     /protein_id="HMA05976.1"
                     /translation="MRLLLPTGSATADLVRRAAEGLDAEVAVTGEIASFLTPEALLSL
                     VRRGRYDLVLVSGMCTASFEGVERETGVPVYRGPRHAADLPLVLPLLGGIELSRTIPA
                     DELLSRKKAEEAARELAERETEASGDLLIRGVKIGGTSRMKVLAEIMDAHRREDLAIE
                     VERLFSEGADIVDLGFGFDATPEDVARCFAALAGIGGPLAADTQDPALIRAALPRADL
                     ILSLQGGNIPVVGAEVAAAGAAVVVIPGEGSLEENLAAARAAGIGAVIADPILPPVGS
                     GFTDALARFGAPGGLLFFGAGNVVELLDGDSVGANALLAGIAMEVGAAIIFTSEHSDK
                     TRGSVAEMRRATEMMVLTRGRPYPKDLGIDLLILKEKRRRREPPLVFERSEQARPMPR
                     EVTYDPCGNFRIGIEGKEIVAVHDGVAIRGENWEDVLYTLITEGRVSRLDHAGYLGKE
                     LHKAYLALRFGRSFEQDGEF"
     gene            12537..13202
                     /locus_tag="VKO45_08645"
     CDS             12537..13202
                     /locus_tag="VKO45_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HMA05977.1"
                     /translation="MLRCDLHVHTNFSRDGESSVEDILARATSVGLDAIAITDHDTCD
                     GIQKAEQAGSGLVIIPGVEVSTKQGHLLVLGVRKPLPPGLHVMETIRIARSLGGVTIL
                     PHPYHMWRHGVARRVPESIGAVDAIEVFNSRYIVGSANRKAARIARKVSIAAVGGSDA
                     HNARFVGFGYTWIAAEPEVSSILTAIREGRTISGGRMTPLRTYSRQSLRNALRRMRRR
                     VRP"
     gene            13199..13999
                     /gene="truA"
                     /locus_tag="VKO45_08650"
     CDS             13199..13999
                     /gene="truA"
                     /locus_tag="VKO45_08650"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HMA05978.1"
                     /translation="MRLAFRVAYLGDGFFGSQVQTDQRTVEGEFIAAARRLALFDDWR
                     EARFAFAGRTDRGVHARGQVAAFDTAEPMRAIAALNVQLPRDCWCTGVAEVPGDFHPR
                     YDARSRTYRYYFPRVDLDRESMERAARLFTGSHDFSRLARVEGKDPVRKVLSARVFEE
                     AGAGGDFTCLEVTAESFLWQMVRGMAGALLAVGQGRLDEDGVRELLTGAPGERFPPAP
                     SSGLVLWEVDCGIGFEPLNRSPRSSAFLSSVRTHHEVLARVVSLLEEG"
     gene            complement(14021..15064)
                     /locus_tag="VKO45_08655"
     CDS             complement(14021..15064)
                     /locus_tag="VKO45_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05979.1"
                     /translation="MAKRLRLGRIMFMACAVALIAIIFAGGYLLLSRPAAIYPSIHPV
                     QVEGGTYRNVGMSFRFEGANRSISIPVNGTVYYGARATNKEALLSHDIPDSIFIPAYY
                     LSFLSDPNQEEFFRELSGAFRGLREGTSLDDDESLEMMAVAVQALPYETDGTLTPPKY
                     PIETYTDGTGDCDDKSLLLAGLLAREGYGVALFYFGAETHMAVGIKGYGCEYAGTGYG
                     YVATTNVSLVGVPETNLAGGVNLTSTPLVIPVANGTRLYGACQQTRAIGKALERTGDR
                     AVSLSKDLATLSARMNELRSQGRFGEYNQIAAQYEKAVADFNDNALAHNYILDHQDDR
                     KGTYAWLGARALV"
     gene            15185..15709
                     /locus_tag="VKO45_08660"
     CDS             15185..15709
                     /locus_tag="VKO45_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05980.1"
                     /translation="MVDLRDIPEGTRWQIATRVLSSLPIMYDVAFREAVGKRYDELEY
                     QVWLELGKDAMDIAKAFHLPVSTAAELAGDLRLITRIIFGPEFRYEVLEISPDRATLL
                     TKTCPFLGSTHEYGGTAKHLFPKCLAFSVAVVETLNPKFTARFVRSLCMADRTCEMAI
                     LTKEEAKKIDEPGG"
     gene            complement(15814..>17026)
                     /locus_tag="VKO45_08665"
     CDS             complement(15814..>17026)
                     /locus_tag="VKO45_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HMA05981.1"
                     /translation="GAVLGSKRLKAIAVRGTGKVPRGDPAEFSRVRDAIREKIEKAGL
                     NKGGLHRYGTPNVLSLINEAHLLGTRNFQASHFEEAEGVSGERMRETLVDRVAACYSC
                     SIACGRETRAGGLRGHGPEFETIWAFGPDCGVPDLASVAQANYLCNELGLDTISTGAT
                     IACAMELSARGYLAEDIRFGDGKTVVDLVRLTGYREGIGDRLAEGSLRFATAAGHPEL
                     SMSVKGQELPAYDPRGLQGQGLEYATSVRGGCHVYGNMVYPEVFGIPVRLDPAVDEGK
                     AGWVKHLQDLAAALDSAGVCLFTFRALVPADFAKMVAAVTGTPLSEGGLLAVGERVWN
                     LQRLFNLGAGFTRADDTLPRRLMEEPITSGAAAGRVWRKEPLLSEYYKIRGWDGEGRP
                     TKERLASLGIP"
BASE COUNT         2743 a         5710 c         5774 g         2799 t
ORIGIN      
        1 cggggaagga ccccggagga ggacgagcgg ctggcacagg aactgcgttc cgacgaaaag
       61 gaacgggccg agcacctcat gctcgtggac ctcgcccgga acgacgtggg aagggtctcg
      121 cggttcggct cggtcagggt cgaggatttc atggccgtgg aacggttctc ccatgtccag
      181 cacctggtct ccacggtcca cgggaccctc tcggaccacc tgaccccctt cgatgccctg
      241 aagtcctgtt tcccggcggg gacggtgtcg ggagccccga agatccgggc catggagatc
      301 atcgaggagc tggagccgga gcgacggggt ctctacgcgg gggccgtcgg gtacgcgggg
      361 ttcgaccgga ccctcgagtt cgccatcacc atccgcacca tcgtcgcccg ggggaggcgg
      421 gccgaggtcc aggtgggggc cgggatcgtc gccgactccg accccggccg ggagtgggag
      481 gagaccgaga gcaaggggat ggccctcatc cgggccatcg agctcgtgga gggatccggg
      541 tgagggtcct catcatcgac tgctacgaca gcttcaccta taacctgacc cagctcgtgg
      601 gctcggcggg tgccatcccc gaggtgatcc tctgcgacgc accgcaggat gccctggagc
      661 ggcacagctg ggaccggatt atcctctcac cgggtccggg ccgcccggag gactcggggc
      721 tctgcctcac cgccctctcc accctctccc acgatatccc caccctgggg gtgtgcctgg
      781 gccaccaggc cgtatgcacc gccttcgggg gacgggtcgt gcgggggcag agacccgtcc
      841 atgggaagac ctcgccggtc agccacgacg gaaagggcat tttcaccggt ctcgggaacc
      901 ccttcacggc gacgaggtac cattccctgg tcgccgaacg cgcctccctt ccttccgatc
      961 tcgaggtatc ggcagagtgc cccgaggatg gctgcgtgat gggcgtgcgg caccggagat
     1021 atcccatcga cggggtccag ttccatcccg agagcatcct gaccggcgaa ggcccgcgga
     1081 tcatcggaaa cttcctctcg ggggagggag cgtgaccccc aaggaggcga tccttgccct
     1141 cacctcgggc cgggatctca accgggagga ggcggcctcg gtgatgaggg ccgtcatggc
     1201 cggcgacgcg accccggccc agatcggggc catcctcgcc tccctctcca tgaagggggc
     1261 gcggagccag gagctcgcgg ggtttgccgg ggcgatgcgg gagttcgccg tccccttccc
     1321 ccacagtctc gcgggaccct tcgtcgacac ctgcgggacc gggggggatg gatccgggac
     1381 cttcaatgtg agcacggcag cggccttcgt ggtctccggg gccgggctcc cggtggtgaa
     1441 gcacgggaac cggtcggtga cgagccggtg cggttcggcc gacgtcctca ccaccctggg
     1501 ggcgaggatc gaaattcccc ctgaccggat ggccggcgtt ctccgggcga cgggcatcgc
     1561 cttcctgtac gcgcccctct accacccggc gatgcgccgg gtcatggggg tccggcagga
     1621 gctcggggtg aggaccgtct tcaacctcct cggccccctc acgaatcccg ccggtgcccg
     1681 gatccagctc ctcggcgtgt accggccgga tgccgtcctc accatggcag aggtcctccg
     1741 tcttctcggg gccgagcggg ccatggtggt ccatggagag ggcatcgacg agatcaccac
     1801 cgccgggccg acccgggtcg cggagatcga cggggatggg atccgggagt acaccctcca
     1861 ccccggggac ttcgggatcc ccgtctcctc catcgaggat ctccggggcg gggacgccgc
     1921 ggagaatgcc cggatcctcc gggaggtcct ctccggcgag atgggtgcgg cccgggacgc
     1981 ggtcctcatg aatgcggggg ccgccatcta tcttggaggc ggggctcagt ccatcgccgg
     2041 cgggctctcc cgggccgagg agtccctcga ctccggccag gccctggaga agctcggggc
     2101 cttcatcgat gccacggtgg gagcgtgacc gtgatccttg acgagatcct ggaggcgacg
     2161 gaggagcgag tccgggccct ggaggggagg accttttccc ttccgccggc cgccggccct
     2221 caccggagcc tgaacagggc cgtctgccgc cccccgggac ggaacgccat catcgccgag
     2281 gtgaagtttg cctcgccgtc ccgggggagg atccgcgccc ctacctcccc cgacgggatc
     2341 gcccgggacc tcgtccgggg aggctgctgc gccctctcgg ttctcaccga gcctggattc
     2401 ttcgcggggt ccacggcatc cctcacccgg gtccgggggg cggtcggggt cccggttctt
     2461 cggaaggact tcatcattca tcccgtccag ctcgacgaaa cccgggccct cggggcggac
     2521 gccgtcctcc tcatcgcggc cctcctcggg gaccggcttt cggggttcgt ggaccgtgcc
     2581 ctcgccctcg ggatcgaacc cctggttgag gtgcataccc gggaggaggc cgaggccgcc
     2641 atccggacac gctcgaacct catcggcgtg aacaaccgtg acctcggttc gatggagatt
     2701 gacctcgcca ccaccatcgc cctcgggccg ggcatcagga aggcaggaaa gagggtggtc
     2761 tctgaaagcg ggatccgatc ccccgacgat atccggacgc tgagggattc ctgcgatgcc
     2821 ttcctcgtcg gctcctccct catggcctcg gaggacccgg gaaaaaccct ggaggccctc
     2881 gtatgcgcgt gaagatcagc gggccggtac ctttgcccgg aaatcatcgg gagactcgcg
     2941 tacgggtaaa aatctgcggg atcacgaggg ccgaagatgc catccacgcc gagaaggcag
     3001 gcgccgatgc catcggggtc atcctgttct cggactcccc ccggtgcata agcctggaga
     3061 ctgccggaga gatcttccgg tcggtcgggc cctttatcac gacggtggcg gtgacccaca
     3121 cgaagacccg gaaggatctc gaggaaatca tcactctcca ccccggtgcc gtccagatct
     3181 cccacccttt taccttcccg gccccgcctc cggtaagggt tctccgggtg gtgaaaccgg
     3241 gggatcccct ccccggtgac gccgacgccc tcgtggtgga ccagtccatg ggaacgggaa
     3301 gacactacga tcccggcttt gcccgggaga tggtacggcg ctcgcgggtc cccgtgaccc
     3361 tcgcgggcgg cctcaccccc gggaacgtgg cggatgccat ccgggctatc cggccctatg
     3421 cagtcgatgt ggcctccggc gtggagataa gcccggggat caaggatccc gcgagggtca
     3481 ccgccttcat ccgggccgcg cagggggtgc tcccgtgagg aaaggacggt tcggggcctt
     3541 cgggggccgg ttcgtctccg aggccctgat gggagccctc gccgaacttg aggaggcttt
     3601 cgaacggttc cggaaggacc cggccttcct ctcgtccctc aactcctacc tgaccgagta
     3661 cgcaggaaga cccacccccc tgaccttctg cgcgaacctg tcccgggaca tgggctgccg
     3721 ggtgtacctg aagagggagg acctcctcca cggcggggcc cacaagctga acaacaccct
     3781 cgggcaggcc ctcctcgcga ggcacatggg aaagaagagg ctcatcgccg agacgggcgc
     3841 gggacagcac ggcgtggcca cggccatcgc cggggccgtc ctcggccttc ccgtcgaggt
     3901 ctacatgggg gaggtggaca tcgcacggca gcgccccaat gtcttccgga tggagctcat
     3961 gggtgcgaag gtgatccccg tgtcttcggg gacgagaacc ctcaaggacg ccgtgaacga
     4021 ggccctccgg gactgggcgg cctccgcaaa ggagacccac tacctcctcg ggtccgtggt
     4081 gggaccccat cccttccccc tcatggtccg cgacttccag tcggtcatcg gccgggaggc
     4141 ccgggcccag gtccttgaga aggaggggag gcttcccgac gcggtcatcg cctgcgtggg
     4201 cggaggctcc aatgccatcg ggatcttcca ccccttcctt tcggaccggg tgaggctcat
     4261 cggggttgag gctgggggcg aagggctggg taccggccgg cacggggcct ccctctgcgc
     4321 cgggtcgccg gggattctcc acggggccct ctcctacctc ctccaggacg gggacggcca
     4381 ggtcctctcc acccacagcg tggccgccgg cctcgactac ccgggcgtgg ggcccgagca
     4441 ctcgatgctg aagaactcgg gaaaggccga gtatatcgcg gtcaccgacc gggaggccct
     4501 cgatgccttc accctccttt cccgacggga ggggatcatc cccgccctcg agtccgccca
     4561 tgccctcgcg ggtctcgtga acctgaagga ggagttcggc cgggatgaca tcgtggtcgt
     4621 gaacctctcg ggccgggggg acaaggacct cgccgaggtc gcccgtatcg cgggggagag
     4681 gccatgaccc ggatcgggga gctcttcgcg tcgctggacc ggccggcctt catcgcctac
     4741 ctggtgaccg gggatccgga cccgggtggg ttcgccaccg ccgtccgggc ggtcctcgat
     4801 ggaggcgcag acattcttga gctcggcatg cccttctccg atcccgtcgc cgacggcccg
     4861 accctccaga gggctcacat ccgtgccctc gaaggcggga tgaccacgga caggtacttc
     4921 gaggcggtcc gggagatccg gcgggactcg gagatccccc tcatcctcat ggtgtactgc
     4981 aacaccgtcc accggcgggg tgttgatcgg ttctaccggg aagcgaggga tgcgggtgcg
     5041 gacggagtac tcgtcgtgga catgcccccc gaggaaggcg agcgggccct cgcagcagcg
     5101 aagatgaacg acctcgacca gatcttcctc attgccccct cgacctcccg ggaaaggctc
     5161 gacctcatcg cgaaatcggg gagcgggttc ctctacctcg cctcccttct cggggtcacc
     5221 ggcgcccgcg aacgtctctc cgacgaggct tcctcgctca tcggccgggt ccgcccccgg
     5281 acccggctcc ccctcgccgt gggcttcggg atctcccggc cggagcacgc gggagctctc
     5341 gcgaaggcag gggccgatgg gatcatcgtc ggatcggcca tcgtgaagat cgtggaggag
     5401 cggcaccggg acggggcacg gatggccggg gagatccggg actttgtcgc ggggatgcga
     5461 agggccctcg aagggtgagg gctcctcaag tacccaataa ggctcggatc tcgcccagga
     5521 cctccccgaa cttcatgtcc cgatcctccc gggtcatggt gatgaccctc acatcgggcc
     5581 tcttcttgac cctctcgatg gccggagtcc cccgcagcgc gatggtggca agaaggggga
     5641 cgggagaacc gaggatctcc cgcatgagat tcccgaacct ctccgacagg cactccatct
     5701 tcccgatctc gtcgatgaca acgacgcggg tgcccgggtc cccgagcctg agctccccga
     5761 ggaaggattc gaagccaccg gtgtccaccc cgtacttccc gatgcgaagg ggacccttta
     5821 ccgttacgtg ggagaggatc cccctcttcc cgtcgaggct gacgagggca aaaccgactc
     5881 gctccccctt ctcacgaatc tcctcggtgt agaagccggc catcgggaga tcccccagac
     5941 tctcaacaac acgccggacc agggtcgtct tcccgcaccc gggagcgccg gttacgaaga
     6001 ggttcttgat catccctctc ggacaccctc tgttctgccg tgaaatcccc cgagagctcc
     6061 gagcagctgt accgtccccc ccttatcgag cggtgtgttg tcgttcccgc acttcgggga
     6121 gtagatgcat gccgggcagc cggcctcgca ccggcactcc ctcaccgcct cgcaggccat
     6181 cgatgcgatc cggtccatga gctcgaaggc cttctcagcg aggccgatcc ccccctcgta
     6241 gccgtcatac acgaaaatag ccggcccgtc ggtgtccggg tgggccggag tggacacccc
     6301 cccgaggtcc cggcggtcgc agaggacgtg gaagggtgcg atcccgatga cggcatgctc
     6361 ggccccgtgc agggaacccg cgaggtcgaa tcccccggca acgacggagg ccgccagtcc
     6421 cggcgagagg gtgaaccaga aggcccgggt accgaacttc aggggcggaa ggctcagggg
     6481 ctcgaagcca acgagggcgt cgtacttcat gatccggtag ccggtgtact gctcggtcac
     6541 ctccacgtca ccaaggcaca gggtaaagcc ctccccctcc cgacgggaga tttcccgcac
     6601 caccctgaga tccacggtct tcagggcatc ggtgtagtag tccacgtcag cccgctcaac
     6661 ccggatcctc cgggtgtcaa ggtccatctc gcgtacgagg taggtctcgc cccggtggag
     6721 gacgaccgcc ccctcgtggg cctcccggta ggcctgggcg cggtccaggg tctcgaggag
     6781 tgtgccgttg caggtcatcc ggaaggtctc ggaggagatg gagtcaagct tcaccgcctc
     6841 ggcggcccgc cccctcccgc agtagaccgc gccgtgcggg gtcgggcgga ggaggtgggc
     6901 ctcggtgagg gagcggatgg ggggatcgag ctcccccggc cagagggggc ggtcccggtc
     6961 cgggttcacg ggcagctccg aggcggcgca gaggaggtgg ccggagacga tgtacgggtt
     7021 cgcgaggtcg acgatggcat gctcgtgggg gctcccgaag aaggtgtccg ggtggcgcat
     7081 gaaatactgg tcgaggagat cctggaaggc gatgaggaag gcggccgagt catttccccg
     7141 acgcccggcc cggcccgcct gctgccaggt ggagatgatg gtcccgggga agcccgaaat
     7201 gaggactgcg tcgaggcccc cgatgtcgat cccgacctcg agggcgttcg tggaggtgag
     7261 gcccctgagc cggcccgact tcagccggtc ctcgatggcc ctcctctcct cgggaaggta
     7321 ccccgcccgg tacgcggtga cctggccggg gtccctcccc ttccgggcca ggtcctcgtg
     7381 cgcccacatg gcgatgagct cggccatgcg ccgcgagacg gtgaaacaga gggtctggag
     7441 ctcgtgggag aggcaggaga gaaggagttc ctcggtctcc cggtggaccg acttaccgtt
     7501 cacaccatcg gggaaggggt tgtagaggac gaagtgcttc ctcccccggg gggacccgtc
     7561 ggcgtgcacg tggtggtagt catggccggt caggttcctc ccgaactcga gggggtttgc
     7621 gatggtggcc gtggagagga cgaactgggg gtccgccccg tagtgcctgg cgatccgccg
     7681 cagcctccgg atcaggtagg ccatgtggga gccgaagacc ccccggtacc ggtgtgcctc
     7741 gtcgatgacc acgaaggaaa ggttccggaa gaaccgctcc caccggtgat gccagggaag
     7801 gacctgatgc agctcgtacg ggtttgagag gatgatccgg gagcgctccc ggatgagggg
     7861 ccgccgggag gagggagtgt ccccgtcgta gatggccccg gcggtggcga tacccgtccc
     7921 ctcctcgagc ccctggatgg ccttcagctg gtcgttcgcg agagccttcg tgggatagag
     7981 gtagagagcc gtcgcctcgg gccggtcatg gagggcctcg aagatgggga ggttgaaggc
     8041 gagggtcttc cccgaggccg tcggggtggt gatgatcagg ttctccccgt ccctcatggc
     8101 ttccagcgcc tcgacctggt gggtgaagag ccggatcccc ttcctgtcga ggtacctgtc
     8161 aaggacggga ggaaggggcc tcaccggggt cgcgtagttc gcctcctgag ccggaatgac
     8221 ccgcacatgg gccaccctcc gggcctggcg gggatccttt tccagggatg cgatgaggtc
     8281 aggaacgggc atggagatcc tcaaggaggg tcgagagcag gtacccgagg gacaccaggt
     8341 cctggcggtt gtggtcaacg atgggaacga gaggcccgca gttgccggtc cggaggaagg
     8401 cctcgtaaaa ctccgggacg aggctccccg ggatatcgac ccccctggat atcccgaaga
     8461 cgtggcgctc gagggtcgag agccggcagt cggggagctc cctcccccat cggcgccggg
     8521 agaagtggag gaggtcgaag tggggccggt ccagccggac gggcatcccg tagtaggccg
     8581 cccgctcgcc gatatagggg atgtcgaagc tcttcccgtt gaaggaaacg taggccgccc
     8641 cttcgcccgt gaggtcctcc agggcggcat tcagggcggc gggctcctcc gagatctccc
     8701 ggacgaggta ctggctcgtc gcgagagtgg agcccgcgag ccgtccgatg ccgatgagga
     8761 cgatgggacg ggagaagatc cccagggtct cgatgtcgag gaagaccagc tcgtcccggc
     8821 ggtggaggcc gcagggggcg agggagaggg ggtgcgagcg gggaaaccac cgggagatcc
     8881 atcccgcgag gggaacggtc tctgcccgat cgagcatcgc gaggaactcc cgggcgcccg
     8941 gcccgaaccg ccggtgatcc cgcaggtcga ggatggaacg gcacccccgg ccccggagcc
     9001 ttttttcggt caccgccccg atcccttcca ggagccggag ctccccgagg aggcactccg
     9061 tcacccggtt ccggtcgggg tggaggtccg ggacctctgt ccgggcctcg atgcaccagg
     9121 cggggccgaa accggtctcc acctcccttc ccgggagcac ctgccggacc ggtcttccct
     9181 cgtggaggga tatcatgctc cccaggagct ccagggccct cccgtgctcg gaggagaaga
     9241 cgtaggaatc gaggaatgag gtgcggaaga tcttcccgtc ctccaccacc tcgtagcccg
     9301 ggaccgcgtc cacggccgcc cgccacctct ccgagacgcg atggagggtc tggagcatca
     9361 cggaggagtg gtcgccggct cttttagcgt ttcacggcgc ggggtgaaag tatgcccgcg
     9421 gtcacctccg gtgaccgagg gccattcctg gcggaatgcc cctcactcaa ggaggacgag
     9481 cccgacggag acgatgtccc cgtggacgat cttcccgtac tcgaggttct tgcaggggat
     9541 ggtggagagg tcccagccgg cgttcttcgc cgtggcaaag acatccatgc ccgcggcctc
     9601 catgctcggc cggacgagat ccatgtgccg gcacaggcgc cggatggact cgtccacgac
     9661 ccccggctgc tcctcggcga cgcagtgctc gcagaagatg caggggtagg gcccgaagcc
     9721 gaaggccttg tagaacccgt cgtagaaggc cgtcttctcg aggaggtgga cggtggtgtg
     9781 gccccagagg atcaggtccc ggtagaaggg gtggaagtcc tcggggatct cctcgggcct
     9841 gaggtgtttg tgccccggga tcccgtcgaa gcggacgagg attcccgtcg agtaccccgc
     9901 taccatgcgc cgggtctcct ccgggctcgg ggcgtacggc gggcaggaga ggtgtttccc
     9961 gtatcccttg cagccgtacc ggcacttgta cctcacccat tccgcgacga ccaatgaatc
    10021 cgccgagatc acggtggtct tcgcatcctt ctcccgggcg agtgccgaga gcttcccgat
    10081 ctcctcctcg agttcgcggc gcataatctc ctcaaagggg tgatgggggg gcagggctat
    10141 aaagaggttg tgaacttccc cctcatcagg tggatctact tcccgcgggt ctcaagaatt
    10201 ctctcgatga tcttccgggt gctggaacac ggatctgcgg agtactcccc gatccgaacc
    10261 acccgggctc cgattcccct ttccttcagg gctatggtaa gatcctcctc ggagaagtgc
    10321 tggttgaacc cgagggtgat gatggcgggg gctatctcct cgatgggccg gaacatgtcg
    10381 atcggatccc cgagcctcgc atggtcgacc attctcaggg cctggacgac cctgagccgc
    10441 tgggcctccc cgatgaccgg cctcggcttg tgccggacgt tcgcatcccg ggcgacgatg
    10501 acatggagct cctccccgag cttccgggat tcctccaggt agtagatgtg ccccgggtgg
    10561 atgatgtcga aggtgccggt ggccaccacc ctcttcattc caccacctcc agctcgcagg
    10621 gttccccgtc ggaggtaaag cagcgccagc tcgtctcgga aaacgggggg cccaggatga
    10681 ggtggtagcg cccgagggcc gggaagaacc gcaggtcggc atccgagggc cggagggccc
    10741 cgctcgggtg ggagtgggcg ctcccggcga cgtggagacc gaggggcatc aggtcgtacc
    10801 ggaggctcgc gctctgtgtg gtggaggtga aggggaggaa gtagaactcc cggatcaccc
    10861 cgtcctcctc cctgagcacg gccacgaact cccggcctcc tccctcccgg tcgacctccc
    10921 gggccgactc gaggagggcc tggaggaggt cgcgggcgat gccccggatc ttcatgcctg
    10981 tggtactggg aaggggggag tgatgaagga ttccctgccc ggagtttcac ggggtgagtc
    11041 tagaactccc cgaaggcagt actatcgtcc tgcccgggga gttaaaactc cccgtcctgc
    11101 tcgaaggagc ggccgaacct gagggccagg tacgccttgt ggagctcttt tccaaggtac
    11161 ccggcgtggt cgagccgcga taccctcccc tcggtgatga gggtatacag cacgtcctcc
    11221 cagttttccc cccggatggc aaccccgtcg tggaccgcga cgatctcctt tccctcgatc
    11281 ccgatccgga agttcccgca cgggtcatag gtgacctccc ggggcatggg ccgggcctgc
    11341 tcggatctct cgaaaacgag cggaggttcc ctcctccggc gtttctcctt caggatgagc
    11401 aggtcgattc ccaggtcctt cgggtacggg cggccgcggg tgaggaccat catctccgtc
    11461 gccctccgca tctcggccac cgatccccgg gtcttgtccg agtgctcgct tgtgaagatg
    11521 atcgcggcgc cgacctccat ggcgatcccg gcgaggaggg cattcgcgcc gacggaatct
    11581 ccgtccagga gctccacgac attccccgcc ccgaaaaaga ggagcccccc gggtgccccg
    11641 aacctcgcca gggcgtcggt gaacccggac ccgacggggg gaaggatggg atccgcgatg
    11701 acggccccga tccccgccgc acgggcggcg gcgagattct cctcaaggga tccctccccg
    11761 gggatcacca ccaccgccgc ccccgcggcc gcaacctcgg ccccgacgac cggaatgttc
    11821 cccccttgca gggagaggat gaggtcggcc cggggaaggg cggcccggat gagggcggga
    11881 tcctgggtgt cggcggcgag aggtccgccg atgccggcga gtgccgcgaa gcaccgtgcc
    11941 acgtcctccg gggtcgcgtc gaagccaaac cccaggtcca cgatatctgc tccctctgag
    12001 aagagccgct ccacctcgat ggcgaggtcc tctctccggt gggcgtccat gatctcggcg
    12061 aggaccttca tccgtgaggt ccccccgatc ttcacccccc ggatcaggag atccccggat
    12121 gcctccgtct ccctctctgc cagctcccgg gccgcctcct ccgccttctt ccgggagagg
    12181 agttcatccg cgggaatggt gcgggagagc tcgatcccgc cgaggagggg gaggacgagg
    12241 gggagatccg ccgcatggcg cggtccccgg tacacgggca ccccggtctc cctctcgacc
    12301 ccctcgaagg atgcggtgca catgcccgag acgaggacca ggtcgtacct gccccgccgg
    12361 acaagggaga ggagggcctc cggggtgaga aaggaggcga tctccccggt caccgccact
    12421 tcggcatcca ggccctctgc cgcccttcgc accaaatcgg cggtggcgct gcccgttgga
    12481 aggaggaggc gcataagtgc gtttaatatg cggaaggtga aaaaatgatc ctgttcatgc
    12541 tccgctgcga cctgcacgtc cacacgaatt tctccaggga cggggagagc agtgtggagg
    12601 atatcctcgc ccgggccacc tcggtggggc tcgatgccat cgccatcacc gaccatgaca
    12661 cctgtgacgg tattcagaag gccgagcagg ccggatccgg cctcgtcatc atcccggggg
    12721 tcgaggtctc cacgaagcag gggcacctcc tcgtcctcgg ggtgcggaag cccctccccc
    12781 cgggcctcca cgttatggag accatccgga tcgcccggag cctcgggggg gtcaccatcc
    12841 tcccccaccc ctaccacatg tggcgccacg gggtcgcgag gagggtcccg gagagcatcg
    12901 gggccgtgga tgccatcgag gtcttcaaca gccgctacat cgtcggctct gcgaaccgca
    12961 aggccgcgag gatcgcccgg aaggtgagca tcgccgcggt cggcgggagc gatgcccaca
    13021 atgcccggtt cgtcggcttc gggtatacct ggatcgccgc cgagcccgag gtctcctcca
    13081 tcctcaccgc catccgcgag gggaggacca tctcgggcgg gaggatgacc cccctgcgta
    13141 cctactcccg acagtccctc aggaacgccc tccggaggat gagacgcagg gtccgcccat
    13201 gaggctcgcc ttccgggttg cctacctggg ggacggattc ttcgggtccc aggtccagac
    13261 cgaccagcgg accgtcgagg gcgagtttat cgccgcggcc cggaggctcg ccctcttcga
    13321 cgactggcgg gaggcccggt tcgccttcgc cggccggact gatcgggggg tccatgcccg
    13381 gggccaggtc gccgccttcg acaccgccga gcccatgcgg gcgatagcgg ccctcaacgt
    13441 ccagctcccc cgggactgct ggtgcacggg ggtggcggag gtccccgggg acttccatcc
    13501 ccggtacgat gcccggtccc gcacgtaccg gtactacttc ccgagggtgg acctcgaccg
    13561 ggaatccatg gagagggccg cccggctctt caccgggagt catgacttct cccgccttgc
    13621 ccgggtggag ggaaaggacc cggtccggaa ggtcctctcg gcccgggtct tcgaggaggc
    13681 aggtgccggc ggggacttca cctgcctcga ggttacagcg gagtccttcc tctggcagat
    13741 ggtgcggggg atggcgggtg cccttctcgc cgtggggcag ggaaggctcg acgaggatgg
    13801 ggtgagggaa ctcctcaccg gcgcaccggg cgagcgcttc ccacccgcac cttcatcggg
    13861 gctcgtcctc tgggaggttg actgcgggat cggtttcgaa cccctcaacc ggagcccgcg
    13921 gtcctcggcc ttcctctcct ccgtgagaac ccaccacgaa gtgctggccc gggtcgtctc
    13981 cctcctcgaa gaaggatgag ggctgccttt tggcggcctt tcagaccaga gcgcgggcgc
    14041 cgagccaggc gtaggtgccc ttccgatcgt cctggtgatc gaggatgtag ttgtgggcga
    14101 gggcgttgtc attgaagtcc gcaaccgcct tctcatactg ggccgcgatc tggttgtact
    14161 ccccgaacct tccctgcgat cggagctcgt tcatcctcgc ggacagcgtg gcgagatcct
    14221 tcgagaggga gactgcccgg tccccggtcc tctccagggc cttcccgatg gcccgggtct
    14281 gctggcaggc cccgtagagg cgggtgccgt tcgccaccgg gattacgaga ggtgtggagg
    14341 tcaggttcac ccctccggcg aggttggtct ccgggacccc tacgagagag acattggtcg
    14401 tcgccacgta cccgtagccg gtgcccgcgt actcacatcc ataccccttg ataccgacgg
    14461 ccatgtgggt ctcggccccg aagtagaaca gcgcgactcc atatccctcc cgtgcgagga
    14521 ggccggcgag gaggaggctc ttgtcgtcgc agtccccggt gccgtccgta taggtctcga
    14581 tgggatactt cggcggcgtg agggtcccgt cggtctcgta cgggagggcc tggaccgcca
    14641 ccgccatcat ctccagggac tcgtcgtcat ccagggatgt tccctccctg agcccccgga
    14701 aagcccccga gagctcgcgg aagaactcct cctggttcgg atcggagagg aaggagaggt
    14761 aataggccgg gatgaagatg ctgtccggga tatcatggga gaggagtgcc tccttgttcg
    14821 tcgcccgggc gccgtagtaa accgtcccgt tcaccgggat cgagatggac cggttcgcgc
    14881 cctcgaaccg gaagctcatc ccgacattcc ggtacgtacc tccctcgacc tggaccgggt
    14941 ggatggaggg atagatcgcc gccggcctgg agaggaggag atagcctccg gcgaagataa
    15001 tggcgatgag agccacggca caggccatga acatgattct cccaagccgg agacgcttcg
    15061 ccatgtggtc cctttcccct aaagggtatt cgcccggccc catttcaact taccgcggga
    15121 gggagaatat atatccccca tggggaaatg tcacgtagaa cggcagttct ggggctgatc
    15181 aggcatggtg gatctgcggg acatccccga gggaacccgg tggcagatcg cgacgagggt
    15241 cctctcctcg ctcccgatca tgtacgacgt ggcgtttcgg gaggcggtgg gaaagaggta
    15301 cgacgagctc gagtaccagg tctggctgga gctcgggaag gatgccatgg atatcgcgaa
    15361 ggctttccac ctcccggtct ccaccgccgc ggagcttgcc ggcgatctcc ggctcatcac
    15421 gaggatcatc ttcggccccg aattccgcta cgaggtcctc gagatctctc cggaccgggc
    15481 aaccctgctc acgaagacct gcccgttcct ggggagcacg cacgagtacg ggggcacggc
    15541 aaagcacctc ttccccaagt gcctggcctt ctccgtcgcc gtggtggaga ccctgaaccc
    15601 gaagttcacc gcccggtttg tccgctccct ctgcatggcg gaccgcacct gcgagatggc
    15661 cattctcacc aaagaagagg cgaagaagat cgacgaaccg gggggctgat cctcagggga
    15721 ggcccgagat cggagatccc gggaccccga ggattgtgag tcctcaggga gccccgtgat
    15781 tggatatccc gggaccccga ggactgtgag tcctcagggg atcccgagag aggcgagccg
    15841 ctctttcgtt ggccgcccct ccccgtccca gccccggatc ttgtagtatt ccgagaggag
    15901 gggctccttc ctccagaccc tcccggccgc ggctccggaa gtgatgggct cttccatcag
    15961 ccgccggggc agggtgtcgt cggccctcgt gaaccccgcc ccgaggttga agagcctctg
    16021 gaggttccag acccgctccc cgacggcgag gaggccccct tcggagagcg gtgtgcccgt
    16081 gaccgccgcg accatcttcg cgaaatcggc ggggacgagg gccctgaaag tgaagaggca
    16141 gacacccgcc gagtcgaggg cggccgcgag atcctgaagg tgcttcaccc accccgcctt
    16201 cccctcgtcc acggcggggt cgagtctgac cggtatcccg aagacctcgg ggtagaccat
    16261 gttgccgtag acgtgacacc cgccgcgcac cgaggtggcg tactcgagtc cctgcccctg
    16321 gagcccccgg gggtcgtacg cgggcagctc ctgccccttc accgacatgg agagctcggg
    16381 gtggcccgcc gcggtggcga accggaggga gccctcggcg agccggtccc cgatcccctc
    16441 ccggtacccg gtgagccgca cgaggtcgac cacggtcttc ccgtccccga accggatgtc
    16501 ctcggcgaga tacccccggg cggagagctc catggcgcag gcgatagtcg ccccggtgga
    16561 gatggtgtcg aggccgagct cgttgcagag gtagttggcc tgggcgacgg aggcgaggtc
    16621 agggaccccg cagtcgggcc cgaaggccca gatggtctcg aactcgggcc cgtgccccct
    16681 gagccccccg gccctcgtct ccctcccgca ggcgatgctg caggaataac aggcggcgac
    16741 ccggtccacg agagtctcac gcatcctttc accggagacc ccctcggcct cctcgaaatg
    16801 gctggcctgg aagttccggg tcccgaggag gtgcgcctcg ttgatgaggg agaggacgtt
    16861 cggggtcccg taccggtgca gccctccttt gttcagcccc gccttttcga tcttctccct
    16921 gatggcgtcc cggacccttg agaattcggc cggatccccc cggggaacct tccccgttcc
    16981 ccgcacggcg atggccttca ggcgcttcga cccgaggacc gccccc
//
LOCUS       DATGDI010000231         6686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_170794, whole genome shotgun sequence.
ACCESSION   DATGDI010000231 DATGDI010000000
VERSION     DATGDI010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170794"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VKO45_08670"
     CDS             complement(<1..308)
                     /locus_tag="VKO45_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05982.1"
                     /translation="MPPQSPRDRRITAIIFDMDNTLFDFVAAKQHACQEVAKFLGRDD
                     WEALFSCFIDSKHGFESHENIKDYFDLCGLLEACSVRKESDETEEKPGALSPIPSSSP
                     "
     gene            complement(446..640)
                     /locus_tag="VKO45_08675"
     CDS             complement(446..640)
                     /locus_tag="VKO45_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05983.1"
                     /translation="MKLGPRDIAEDKGRELAKSMVRESFRDLILADPEFRDSIRPVLK
                     SLIREILREERILPPLRPGR"
     gene            complement(752..1204)
                     /locus_tag="VKO45_08680"
     CDS             complement(752..1204)
                     /locus_tag="VKO45_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05984.1"
                     /translation="MDRYLLELNPETTKRLEQLKSYPEEPYDHVINRLIDSRESNVSL
                     TREEVDEIREALRRLKEGQVPGQENVTAFFTQDPGAPQSSVADPRAVKDMEQAVKDME
                     MIFSGAEKRARDQELTEADYGDTDSVDIHEIDPYDKRGRARTPHLDHL"
     gene            complement(1336..2013)
                     /locus_tag="VKO45_08685"
     CDS             complement(1336..2013)
                     /locus_tag="VKO45_08685"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HMA05985.1"
                     /translation="MNRLTARALLGLVNLLLILLLLLFVPAWSLTYWEGWLFILVFFL
                     PVLLITVYFLEKDPGLIESRLQAGPIAEKERSQKIIQSFAGIFFVLVFIVAGTDHRLG
                     WSEVPLSLVIAGDILVVLGLSIVFLVFRENSFTSGTIEVGKDQEVISTGPYAVVRHPM
                     YAGASLMLLGIPPALGSWWAFPFVFLLIAVIAGRLLDEEKFLSKNLAGYAEYSRKVRY
                     RLIPFVW"
     gene            complement(2062..3630)
                     /locus_tag="VKO45_08690"
     CDS             complement(2062..3630)
                     /locus_tag="VKO45_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA05986.1"
                     /translation="MIWISLGITVYDGANCIGGNKIYLDDGKDGVFLDFGKNFGKYAR
                     FYEEFLKNRPTRGIHDLLALGLIPKLNIYRRDLVTPDISLSGYPSLPISAVLLTHAHL
                     DHCGNIGLLPQEIPVFSTPMTAAILKGYQDTGKSTTEADVAYTSERRPNADDPLYIES
                     DRGSCYLGKEFCLTSEPPEKFLEFLSWRPGQDGKNAKKLEPGRCCTIENHPLPFEVSA
                     VEVDHSLYGACGFLIGGEKTVAYTGDFRLHGRNEAKTREFITRAKGADVLITEGTRVS
                     DAGPEDGSRVTEDSVCETCRGAIEGFPGLVIADFSARNFERLEAFRDIAAETGRELVV
                     TARDLYMLYALFTVDGVRRWEGVQVYDEIVDHRKRKWEAELVIPLAGDRYVRHDEIRG
                     DPGRFILCFSFFDMNHLLDIRPSGGRYLYSSCEPFNEEMEIDFRRLWEWLRFFGIDPC
                     GFSMEKDSSGVVVPVMDRHYHASGHASGADVAWAIHEIDPDVIIPVHTENPGWFEENF
                     DGVVVPREGERYVA"
     gene            3994..4308
                     /locus_tag="VKO45_08695"
     CDS             3994..4308
                     /locus_tag="VKO45_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05987.1"
                     /translation="MNKMGGIPVAGNRRYDNAYLVCTYLTGQNLTRAEVTARDIVSFH
                     RIPESCVFSISALLNFLQRNHIRSSRYGFFISGTQAFKRSGYPHRYRIELLDGPAVSL
                     HL"
     gene            4368..5048
                     /locus_tag="VKO45_08700"
     CDS             4368..5048
                     /locus_tag="VKO45_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HMA05988.1"
                     /translation="MAGAGKKDSARIQVTENGPYLVTGGIPLVKQILVCDREGNSIGW
                     KEGGRYPVPETYSLCRCGRSKTRPFCDGSHTKSHFDGTETASRVPYLDRAEWTEGPDI
                     RLSDAPGFCSHARFCVRGEGIWDLIEQSGDEEARKTALEMAANCHSGRLVVWDRRTGK
                     PIEPAFEKAIGLVEGPVGGMQGPLWVRGGIPVVAADGFPYEVRNRVTLCRCGKSGNKP
                     FCDGAHLR"
     gene            5078..5665
                     /locus_tag="VKO45_08705"
     CDS             5078..5665
                     /locus_tag="VKO45_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05989.1"
                     /translation="MEAVRGETGQAGRRLAVFVAIVIANTLLSAFAVVGAGIAPGIAS
                     FYLAVGFMIAFALWFGGWGVLAAYLGCILGAGLVAGVPGWLNPVWSLADLWQVLIPLA
                     AFRAFHADVGLSTRRDFGIFLAFGWFANNLAGAAWGSVTLALGGVIPWGGVEAAFVSW
                     LAGNLFMTMIITTLLLVFVTPRVREAGILVTGTWA"
     gene            5734..6597
                     /locus_tag="VKO45_08710"
     CDS             5734..6597
                     /locus_tag="VKO45_08710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMA05990.1"
                     /translation="MATLSRSIADPPGIAMKTILVTGSTDGIGKATARALARQGHRVL
                     IHGRDSAKVRTVVGEIGKETGSAEPDHFTADISSFAGVRSLVDQVGDRYDRLDVLVNN
                     AGVYMGERVLSADGLEMTFAVNVAAPFHLSRLLTPALEAAAPARIVNVASGAHFDVRG
                     MDWENLMGEKKYDGWGAYCQSKLGMVLLSFALAGRLDPEKITVNCLHPGVICTKLLYS
                     AFPVYPCDPPETGARTPVYLATSGEVSGTTGKYFDNMRETRSSQASHDRGAQDRLWKY
                     LGGLVDLDRRD"
BASE COUNT         1287 a         2047 c         2149 g         1203 t
ORIGIN      
        1 tgcggcgaag aggatgggat gggagagaga gcccctggct tttcctctgt ttcatccgat
       61 tccttacgga cagaacatgc ctcgagtaaa ccacacaggt caaaatagtc tttgatgttc
      121 tcgtggctct cgaagccgtg cttcgaatcg atgaagcagg aaaagagggc ctcccagtcg
      181 tcccggccga ggaacttcgc tacctcctgg caggcgtgct gcttcgccgc cacgaagtcg
      241 aagagggtgt tgtccatgtc gaagatgatg gccgtgattc tccgatcccg ggggctctgg
      301 ggcggcatat tctctttcca tacatattcg ccgcgcgatg agataaggac aaggatggtc
      361 ccggaggccc cacggatagc aaccggaaaa aggatctgga aggtgatgtg atcaggggaa
      421 tcccgggctt ctcccggctg agaccctatc tgccgggtct caggggcggg aggatgcgct
      481 cctcccgcag tatctcccgg atgagggact tgaggaccgg ccggatggaa tccctgaact
      541 ccgggtcggc gagaatcagg tcccggaagc tctcccgcac catgctcttg gcgagctcgc
      601 ggcccttatc ctcggcaatg tcacgcggtc ctaatttcat cacctacacg atgccggccc
      661 cgttcatatg cgtttccatc cgatccgtca gagatagaga tgttggacgg tcgcggagcg
      721 ccgggtacag catcgcgggt ctggaaaagc ctcagagatg atccaggtgc ggagtgcggg
      781 ctctccctct cttatcgtag ggatcgatct catggatatc gacggagtca gtgtcgccat
      841 agtcagcttc ggtgagttcc tggtcccggg ctctcttctc cgctcccgag aagatcatct
      901 ccatgtcctt caccgcctgt tccatgtcct tcaccgcacg gggatccgcg acggaagatt
      961 ggggcgcacc cgggtcctgg gtgaaaaacg ccgtcacatt ctcctggccc gggacctgcc
     1021 cttccttcag cctgcggaga gcctcccgga tctcatccac ttcctccctc gtgagcgaga
     1081 cgttactctc gcgggaatcg atgagccggt tgatgacatg gtcgtagggt tcctccgggt
     1141 acgacttcag ctgctccagc cgtttcgtcg tctcggggtt gagctccagg aggtacctgt
     1201 ccatgatcag tatctccctg caacgacctt aaaaaccgat ggccgtttcc gcggcggcag
     1261 gacgcggtgg agcgagatgc gtcccgccat cagcgggaaa gacaccgcaa aggcgggcgg
     1321 gttctcccgg tatctctacc agacaaacgg tatcaaccgg tagcggacct tccgtgagta
     1381 ttctgcatac cccgccaggt ttttcgagag gaatttctcc tcgtcgagaa gcctccctgc
     1441 gataacggcg atgaggagga aaacgaaggg aaacgcccac catgaaccaa gggcaggggg
     1501 gatacccagg agcatgaggg aggctcccgc gtacatggga tgacggacaa ccgcataggg
     1561 accggtggag atcacctcct ggtccttccc cacctcgatg gtaccggagg taaagctgtt
     1621 ctcccggaaa acgaggaaca cgatggagag gcccaggacc acgaggatgt ccccggcgat
     1681 gacaagggag agggggacct ccgaccatcc caggcggtgg tcggtgcctg cgacgataaa
     1741 tacgaggacg aagaagatgc cggcaaagga ctggatgatt ttctggcttc tctccttctc
     1801 ggcgatgggc cccgcctgga gccggctctc gatgagtccc ggatccttct ctagaaaata
     1861 gaccgtgatc aggagcaccg gcaggaagaa tacgaggatg aagagccatc cttcccagta
     1921 ggtgagggac catgcgggaa caaagagtaa caggagaagg atgagaagga gattcacgag
     1981 cccgaggaga gcccgtgcgg tcagccggtt catggtgtcc ccgtacccga agcctggtga
     2041 aggagggtaa ccctcacctc cttacgcgac gtatcgttct ccctcccggg gtaccaccac
     2101 tccgtcgaag ttctcctcga accatcccgg gttctcggtg tgcaccggga tgatgacatc
     2161 gggatcgatc tcgtggatcg cccatgccac gtcagcaccc gaggcatgcc ccgaggcatg
     2221 gtagtgcctg tccatgaccg ggacaacaac ccccgatgaa tccttctcca tcgagaaccc
     2281 gcaggggtcg atcccgaaga accggagcca ctcccagagc cggcggaagt cgatctccat
     2341 ctcctcgttg aagggctcgc aggacgagta caggtagcgg ccgccggaag gccggatgtc
     2401 gaggaggtgg ttcatgtcaa agaaggagaa gcagaggatg aaccggccgg ggtccccacg
     2461 gatctcgtca tgcctcacgt accgatcccc ggcgaggggg atcacgagct ccgcctccca
     2521 cttccgcttc cggtggtcga cgatctcgtc gtagacctgg accccctccc agcggcgaac
     2581 cccgtcgacc gtgaagaggg cgtagagcat gtacaggtcc cgggcggtca ccacgagctc
     2641 ccggccggtc tcggcggcga tatcccggaa ggcctcgagc cgctcgaagt tccgggcaga
     2701 gaaatcggcg atgacgaggc cggggaaccc ctcgatggca ccccggcagg tctcgcagac
     2761 cgaatcctcg gtgacccggc tcccgtcctc cggcccggcg tccgaaaccc gggtcccctc
     2821 ggtgatgagg acatccgccc ccttcgcccg ggtgatgaac tcccgggtct tcgcctcgtt
     2881 ccggccgtgg agccggaagt cgccggtgta cgcgacggtc ttctcacctc cgatgagaaa
     2941 gccgcaggca ccgtagaggg aatggtctac ctcgacggcc gatacctcga aggggagggg
     3001 gtggttctca attgtgcagc accggcccgg ttcgagcttc ttcgcgttct tcccgtcctg
     3061 ccctgggcgc caggagagga actccaggaa cttctccggc ggttccgagg tgaggcagaa
     3121 ctccttcccc aggtagcagc tcccccggtc cgactcgatg tacagggggt cgtcggcgtt
     3181 cggcctccgc tccgaggtgt aggcgacgtc cgcctcggtg gtcgacttcc cggtgtcctg
     3241 gtaccccttc aggatggcgg cggtcatagg agtggaaaag acggggatct cctgcgggag
     3301 gaggccgatg ttcccgcagt ggtccaggtg ggcatgggtg aggagcacgg cggagatggg
     3361 aagggagggg taaccggaga gggagatatc gggtgtgacc aggtcccggc ggtagatgtt
     3421 cagtttcggg atcagcccga gggcgagcag gtcgtggatc ccccgggtgg gccggttctt
     3481 caggaactcc tcgtagaacc gggcgtactt cccgaagttc ttcccgaaat ctaaaaagac
     3541 cccgtccttc ccgtcgtcca ggtagatctt gttcccgccg atgcagttcg cgccgtcgta
     3601 cacggtgatg ccgagggaaa tccagatcac ccgccttccc agtgcctctc ccgggcgcag
     3661 ggaaaaggat ttgcccgatc aatccgtgac agatcccgtg gtgcagggac cggtgcccac
     3721 ggcaaaaaga tcccgctcca cggaaaggat gatccccggc gagacgcaaa cgaatctgcg
     3781 actgctgcgg cctcctgacc gacgaggaca acggatgagg agtgggaaac ctcggggaac
     3841 cggtgcgagg agtgcgccat cgcggagccc gcggacggaa ccctcggtga accctgttga
     3901 ataaaagctc tttattcggg ttttttcata aaacagccaa cgatacaaac ctatatctcc
     3961 gaagcgggcc tatgaacgca gggagagggg aaaatgaata aaatgggggg aattccggta
     4021 gccggcaaca ggcggtacga caatgcatac ctcgtctgca cgtacctgac ggggcagaac
     4081 ctgacccgcg ccgaggtgac cgcccgggat atcgtctcct tccaccggat accggaatca
     4141 tgcgtcttct ccatcagcgc attattgaat tttcttcaga ggaatcacat caggagcagc
     4201 aggtacgggt tctttatctc ggggacgcag gccttcaaaa gaagcgggta cccccaccgg
     4261 tacaggattg aactcctgga cgggccggct gtttcacttc atctgtaagg gaatgccccg
     4321 ggaagaaatc tcaaatacca tgatcccgta gtattctcgt gagcatcatg gcaggtgccg
     4381 gtaaaaaaga ttccgcccgc atccaggtga cggagaacgg gccatacctg gtcaccggcg
     4441 ggatcccgct cgtgaaacag atcctcgtct gcgacaggga ggggaattcc atcgggtgga
     4501 aggagggcgg aagatacccc gtcccggaga cgtattccct ctgccggtgc gggaggtcga
     4561 agaccaggcc cttctgcgac ggctcccaca cgaagagcca cttcgatggc accgagacgg
     4621 cgagccgggt cccgtacctg gatcgggcgg agtggaccga ggggcccgat atccggctct
     4681 cggatgcccc ggggttctgc tcccacgccc gtttctgcgt ccgcggggag gggatatggg
     4741 acctcatcga gcagtcaggc gacgaggagg caaggaagac tgcgcttgag atggccgcga
     4801 actgccactc cgggcgcctc gtggtctggg accggaggac gggaaaaccc atcgagccgg
     4861 cctttgaaaa ggccatcggc ctcgtcgaag gaccggtcgg gggaatgcag ggtcccctct
     4921 gggtccgggg gggcatcccg gtcgtcgccg cggacggctt cccctacgag gtccggaacc
     4981 gggtcacgct ctgccggtgc gggaagtcgg ggaacaagcc cttctgtgat ggggctcacc
     5041 tgcgatgaac tcggaaagcg ccggtggtgg aaagaggatg gaagcagtcc ggggggagac
     5101 aggccaggcg gggaggcggc tggccgtctt cgtggccatc gtcatcgcga ataccctgct
     5161 ctcggccttt gcggtcgtgg gcgccgggat cgcgcccggg atagcatcct tctaccttgc
     5221 ggtgggattc atgatcgcct ttgccctctg gttcggggga tggggagtcc tcgccgcgta
     5281 cctgggctgc atcctcggcg ccggtctcgt cgcgggcgtc cccgggtggc tgaatcccgt
     5341 ctggtccctc gcggacctgt ggcaggtcct catccccctc gcggccttcc gggcgttcca
     5401 tgccgatgtc ggcctttcga cccggaggga cttcgggatc ttcctcgcct tcgggtggtt
     5461 cgcgaacaac ctcgcggggg cggcctgggg ttcggtcacc ctcgccctcg gcggggtcat
     5521 cccctgggga ggggtggagg ccgccttcgt gagctggctc gcgggcaacc ttttcatgac
     5581 catgatcatc accaccctcc tcctcgtctt tgtcaccccc cgcgtgagag aggcgggaat
     5641 cctcgtgacg ggcacctggg cctgaaaggc cccggggttc ccgggaaaac ctttttcacc
     5701 tcgacccccg aaagaacccg tcaggaggaa agcatggcga ccctctcccg ctccatcgca
     5761 gatccccccg ggatcgcgat gaagaccatt ctcgtcaccg gatccacgga cggcatcggg
     5821 aaggcgacgg cgagggccct cgcccgccag ggccaccgtg tcctcatcca cgggagggat
     5881 tccgcaaagg tccggacggt cgtcggggag atcgggaagg agaccgggag tgcggagccc
     5941 gaccatttca cggctgacat ctcgtccttc gcgggtgtcc ggagcctcgt cgaccaggtc
     6001 ggggaccggt acgaccgcct cgacgtcctc gtgaacaacg ccggggtcta catgggcgaa
     6061 cgggtcctct ccgccgacgg cctcgagatg acctttgccg tgaacgtggc cgcccccttc
     6121 cacctctccc ggctcctcac cccggccctc gaggcagctg ccccggcccg gatcgtgaac
     6181 gtcgcctcgg gcgcccactt cgatgtcagg gggatggact gggagaacct gatgggcgag
     6241 aagaagtacg acgggtgggg ggcctattgc cagtcgaagc ttgggatggt gctcctctcc
     6301 tttgccctcg cgggaaggct cgatccagaa aagataaccg tgaactgcct gcatcccggc
     6361 gtgatctgca cgaagctcct ctactcggcc ttccccgtct atccctgcga cccgcccgag
     6421 accggggcga ggaccccggt gtacctggct acgtcggggg aggtctccgg aaccaccggg
     6481 aagtacttcg acaacatgag ggagacccgg tcgtcccagg cctcccatga ccgcggggcc
     6541 caggaccggc tctggaagta cctgggaggt ctcgtggacc tggaccgacg ggactaggaa
     6601 cgggaaccgg gagaagggga aataatataa ccacggaggg ggcactcaca gccggtgagg
     6661 agaccatggc ggagtacgat ctcacg
//
LOCUS       DATGDI010000232         1630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_345199, whole genome shotgun sequence.
ACCESSION   DATGDI010000232 DATGDI010000000
VERSION     DATGDI010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345199"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="VKO45_08715"
     CDS             <1..1183
                     /locus_tag="VKO45_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HMA05991.1"
                     /translation="GSTAGSWGSLFILLGIGVNVCFIPLHTWLPDAYPKAHFVASIFL
                     SVYTTKLAVYLLARVQPPTLVIAYMGALMAVYGVSFAVLQNDMRRLLSYHIISQVGYM
                     VAGIGLFAVLGAGSDVGVLGLNGGLAHLVNNLLYKTLLFMAAGVIIWKTGENLLSRVG
                     GLWKRMPVTAIAFWIAAFSISGIPLFNGFVSKGMVILAAEETSPLLWALLEIASFGTF
                     LSFLKLGYFAFLRPAKTEIREASDPPLTAQVAMAGIAALCLAIGLYPPLLYAILPAPV
                     SYAAYDPVQTLTTLAILGAAALFFFTVGKKVLEPHETGLIDFDALYAAACRSVVDFSA
                     VLWEIFGWVYRRATDAAGALYETGRRAMWGEGRDVNWNLLIFGLTLVILLALATRGVG
                     P"
     gene            1180..>1630
                     /locus_tag="VKO45_08720"
     CDS             1180..>1630
                     /locus_tag="VKO45_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA05992.1"
                     /translation="MSSPVLAALAALSPFLIALGGVLSYFVARALPAKDLRWTGAFSA
                     AWLGAVFLVLAGAGATASFGGVAFLGPILQPSALGIAFALLVTLLGVAAALVSLGRLD
                     PRGPLQLYYPLLLFALAGASAAGFTRDLFTLFVSVELSAIPSYALVAY"
BASE COUNT          204 a          613 c          471 g          342 t
ORIGIN      
        1 gggatccacg gcaggatcct ggggatccct cttcatcctc ctcgggatcg gggtgaatgt
       61 ctgcttcatc cccctccata cctggctccc cgatgcctac ccgaaggccc acttcgtggc
      121 gagcatcttc ctctcggtgt acaccacgaa actcgcggtc tacctcctcg ctcgggtcca
      181 gcccccgacc ctcgtcatcg catacatggg ggccctcatg gccgtgtacg gggtgagctt
      241 cgcggtcctc cagaacgaca tgcgccggct cctctcgtac cacatcatct cccaggtcgg
      301 gtacatggtc gcggggatcg ggctcttcgc cgtcctgggg gcagggagcg atgtgggagt
      361 cctgggcctg aacgggggtc tcgctcacct ggtcaataac ctcctctaca agaccctcct
      421 cttcatggcc gcgggggtca tcatctggaa aactggtgag aacctcctct cccgggtggg
      481 cggcctctgg aaacggatgc ccgtcaccgc catcgccttc tggatcgcgg ccttctctat
      541 ctcggggata cccctcttca acggctttgt ctccaagggg atggtcatcc tcgccgccga
      601 ggagacgagc ccgctcctct gggcgctcct cgagatcgcc tccttcggga ccttcctctc
      661 cttcctcaag ctcgggtatt tcgccttcct ccggcccgcg aaaaccgaga tcagggaggc
      721 atcggatccc cccctcaccg cccaggtcgc catggccggg atagcagcgc tctgcctcgc
      781 catcggcctc tatcccccgc tcctctacgc catcctcccg gcaccggtat cgtacgcggc
      841 atatgatccg gtccagacgc tcacaaccct cgccatcctc ggggcggccg ccctcttctt
      901 cttcacggtc gggaagaagg tcctcgagcc ccacgagacc gggctcatcg acttcgatgc
      961 cctctatgcg gcggcctgcc gctcggtcgt cgacttctca gcggtgctct gggagatctt
     1021 cggatgggtc taccgccggg caacggatgc ggcgggtgcc ctgtacgaga ccgggcgaag
     1081 ggccatgtgg ggcgagggga gggacgtgaa ctggaacctc ctcatcttcg ggctcacgct
     1141 tgtcatcctc ctcgccctcg ccaccagggg ggtggggcca tgagctcccc ggtccttgcg
     1201 gctctcgccg ccctctcccc gtttctcatc gctctgggtg gggtcctctc gtacttcgtg
     1261 gcaagggccc tcccggcaaa ggacctccgg tggaccggcg ccttttcagc cgcctggctg
     1321 ggggccgtgt tcctcgtcct cgcgggggcg ggggccaccg cctccttcgg cggggttgcc
     1381 ttcctcgggc caatcctgca accgtcggct ctggggatcg ccttcgccct gctcgtcacc
     1441 ctcctcggtg tggcggccgc cctcgtttcc ctggggaggc tcgacccccg cggccccctc
     1501 cagctctact accccctcct cctgttcgcc ctcgcggggg catcggcggc cggcttcacc
     1561 cgggatctct tcaccctctt cgtctcggtg gagctctcgg ccatcccctc ctatgcttta
     1621 gtcgcctacc
//
LOCUS       DATGDI010000233         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_259049, whole genome shotgun sequence.
ACCESSION   DATGDI010000233 DATGDI010000000
VERSION     DATGDI010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259049"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VKO45_08725"
     CDS             <1..426
                     /locus_tag="VKO45_08725"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMA05993.1"
                     /translation="YFNVYGPGQDPRGEYAAVIPRFAVNVLRRRPPVIFGDGNQVRDF
                     ISVKDVVRANILAMQRDAVGIFNVGTGDGTSILKLARAIMEIGGLAREPVFEKARPGD
                     IRESIADISRARDLMGFRPRVHLADGLRETLEWFRDHPG"
     gene            591..1199
                     /locus_tag="VKO45_08730"
     CDS             591..1199
                     /locus_tag="VKO45_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HMA05994.1"
                     /translation="MEPQDLKKEFNRLYRATTRRVDEVNVPAMKFLMVDGSCEGGNYE
                     EFRNGIEGLFSLSQAMKAAISKTRIGFEYAVMPLECLWWVRDNDYTPDNRDEWKWILM
                     VVQPEYVTSALAREVLGAMDRTKILPGISKVRFGIFAEGPSVQTLHKGARADKWTTIT
                     RLKVFIEKKGYEVRGRHHEIYISDPRSMTAEKMKMIVRLPIK"
     gene            1244..>2103
                     /locus_tag="VKO45_08735"
     CDS             1244..>2103
                     /locus_tag="VKO45_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HMA05995.1"
                     /translation="MRKSSVITGDLFSAHDMPHHPESHARLLGALCGVPSGIPRYPPE
                     KAHLADVERVHDPEYIRGLEDACARTKTFAMLDPDTYITHASFEVALYAAGGAIGAAE
                     RALSGEHCFALVRPPGHHAERDHAMGFCLLNNVAIAAAKALGTVERVAIVDWDVHHGN
                     GTQQAFFDTDRVLYCSIHRGHYYPYSGRTDERGTGRGEGHTLNAPLAPGATIADYDAV
                     FREVFAPAIRKFRPNLLLVSAGQDILFDDPLGGMEMYPSDFGLLTWRLLKAGGLPLAL
                     VLEGGYGSSHG"
BASE COUNT          405 a          635 c          678 g          385 t
ORIGIN      
        1 tacttcaacg tgtacggccc cggccaggac cccaggggcg agtatgccgc ggtgatcccc
       61 aggttcgcgg tgaacgtcct ccgaaggaga cctcccgtga tcttcgggga cgggaaccag
      121 gtcagggact tcatctcggt aaaagatgtc gtccgggcga acatcctcgc catgcagcgg
      181 gacgccgtgg ggatcttcaa tgtgggaacc ggggacggaa cctccatcct gaaactcgcg
      241 cgggccatca tggaaatcgg gggactggcc agggaaccgg tctttgaaaa ggcccgcccg
      301 ggggatatcc gggagtccat tgccgacatc tcccgggcaa gggatctgat gggctttcgt
      361 ccaagggtcc atctcgccga cgggctccgg gagaccctgg agtggttcag ggatcatccg
      421 ggttgagggt aggccgggtt tggggacagg cgatccgggc ccggggatat taggggatcc
      481 gggcacaagg gggtgctcag gggatcctgg caccgggggg tgctcaggag atccgggcac
      541 cgggcggtct tttatccccc ctcatcctaa atctcctact caggagcagg atggaaccgc
      601 aggacctgaa gaaggagttc aaccggctct accgggccac cacccgcagg gtcgacgagg
      661 tgaatgtccc ggcgatgaag ttcctcatgg tggacgggtc ctgcgagggg gggaactacg
      721 aggagttcag gaacggcatt gaggggctct tttcgctttc gcaggccatg aaggccgcca
      781 tctcgaagac gcgcatcggg tttgagtacg cggtgatgcc cctcgagtgc ctctggtggg
      841 tgcgggacaa tgactacacc cctgataacc gggatgagtg gaagtggatc ctcatggtgg
      901 tgcagcccga gtacgtcacg agcgcccttg cgagggaggt cctcggggcc atggaccgga
      961 ccaagattct tcccgggatc tcgaaggtca ggttcgggat ctttgcagaa ggaccatcgg
     1021 tccagaccct ccataaaggg gcgcgggccg acaagtggac aaccatcacc cggctgaagg
     1081 tcttcatcga gaagaagggg tacgaggtcc ggggccggca ccatgagatc tacatctcgg
     1141 accccaggtc catgaccgcc gagaagatga agatgatcgt ccgccttccg atcaagtgaa
     1201 ccggaccagc ggatttcatc ctggttagga gggggaggtg acgatgcgga agagctcggt
     1261 catcacggga gatctctttt ctgcgcatga catgccccat caccccgaga gccatgcccg
     1321 gcttctcggg gcactttgcg gggttccctc cggtatcccc cggtatcctc ccgaaaaagc
     1381 ccatcttgcg gacgtggaac gagtccacga tccggagtat atcagggggc ttgaggatgc
     1441 ctgcgcccgt accaagacct tcgccatgct cgatcccgat acctacatca cccatgcctc
     1501 cttcgaagtt gcgctttacg ccgccggagg ggctatcggg gccgccgaaa gagcactttc
     1561 aggcgagcac tgcttcgccc tcgtccggcc tccggggcac catgccgagc gggaccatgc
     1621 catgggtttc tgcctcctca ataacgttgc catcgcggcg gcgaaggccc ttggcactgt
     1681 cgaacgggtc gccatcgtgg actgggacgt gcaccacggg aacggcaccc agcaggcctt
     1741 cttcgacacc gaccgggttc tctactgctc catccaccgt ggccactact acccgtactc
     1801 gggaagaaca gatgaaaggg gaacaggaag aggagagggg cacaccctga acgcacccct
     1861 tgcaccaggg gcgaccatcg ccgactacga tgcggtgttc agggaggtct ttgccccagc
     1921 catccggaaa ttccgtccca atcttcttct cgtctcggcc gggcaggaca tccttttcga
     1981 cgatcccctc ggggggatgg agatgtatcc gtcagacttc ggcctcctta cctggcggct
     2041 cctgaaggct ggcggccttc ccctggccct cgtccttgag gggggctacg gatcctccca
     2101 cgg
//
LOCUS       DATGDI010000234         9747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_374857, whole genome shotgun sequence.
ACCESSION   DATGDI010000234 DATGDI010000000
VERSION     DATGDI010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 9747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9747
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374857"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VKO45_08740"
     CDS             <1..602
                     /locus_tag="VKO45_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA05996.1"
                     /translation="HCGGAGLFPAVARLTRTSLECIRARDRAFGSRSEGLILETVYTT
                     LINLFSGFRPPRELEILPEPGPAVTGVFPVISRFTLSGHAFEILQGLNGHMAGQVYLA
                     SRELGVLFTGDSLIHLEGLTPERRSFNSLAVNLITSVNVDRELARRERDGLLVLAASW
                     DRDVPAGGRRCLICGGHGPVSILEGRSLDTCGAVERCNP"
     gene            685..1740
                     /gene="carA"
                     /locus_tag="VKO45_08745"
     CDS             685..1740
                     /gene="carA"
                     /locus_tag="VKO45_08745"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HMA05997.1"
                     /translation="MKAVLGLEDGDSVVGEGFGVEGSCAGELVFCTTMTGYMEALTDP
                     SYHGQILMFTYPLIGNYGVDKGNFQSEGVKALGCVVREICRVPSGGPPLDRYFEEQGL
                     LGITGVDTRRLTIKCREMGTMRAALVVGDDRREYAVECAKKVPLITEVDLVAAVSGRE
                     PHHIPGTGKRIAVLDLGVKRNMLISMMKRGADLHVFPYDTPHDVIQACRPDALFISNG
                     PGDPKRVTAAVECVRDFIGEIPIFGICFGNQITALALGGDTYKLKFGHRGVNQPVRFH
                     DGSIFITTQNHGFCVDGESLPEGCTVSYTNVNDGTLEGFENRDLSISCVQFHPEAHGG
                     PFDTEAHYFDTMFRRIP"
     gene            1740..4910
                     /gene="carB"
                     /locus_tag="VKO45_08750"
     CDS             1740..4910
                     /gene="carB"
                     /locus_tag="VKO45_08750"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HMA05998.1"
                     /translation="MPRKEHIRKVLLIGSGPIQIGQAAEFDFSGSQACKALREEGVTV
                     VLVNSNPATIQTDPEMADTIYIEPLRAETIAKIIEKERPDGILSGMGGQTGLNMTAEL
                     AEKGALKGVEILGTPLEAIYRGEDREQFKNLMQSIGEPIPRSMILRDIRELEEAERLV
                     GLPAIIRPAYTLGGSGGGIAHTKEELRKITEVGLTRSRIHQVLIEESVLGWKEIEFEV
                     MRDAADTCIIVCGMENVDAMGIHTGESVVVAPILTLRDDEFQMLRTAAIKIIRALGVQ
                     GGCNIQFAYEKGDYRVIEVNPRVSRSSALASKATGYPIARVAAKIAIGLHLDEIMNTV
                     TGCTPASFEPAVDYVVVKVPRWPFDKFKTADRTLTTAMKSTGEVMSIGRTVEEAFKKA
                     LRSVDTDVTRHTNAHEIRMILQRPTDERFGCLFDAFREGFTVEEVSGLTFIDPYFLEK
                     VRNVVEMERRLSGAASPVLIRKAKEFGFSNAEIVGLSGLPWGEVENIAGKPTYKMVDT
                     CAAEFPATTPYFYSTWDTECEIIPTENRKVLILGSGPIRIGQGIEFDYCTVHAVKAVR
                     DEGVEVHIVNNNPETVSTDFDTSDRLFFEPMHLEDVVNILKKDSYGGVMVQFGGQNSI
                     NLALPLEKEIARLGIPTKILGTSPDAMDIAEDRDRFSEFLDRLRIPSPPNSSAYSEEE
                     AFSIAERIGYPVLVRPSYVLGGRAMEIVHDSADLATYIKEAVRVSRKHPVLIDLFLQN
                     AVEIDVDAACDGEDVLIGGIMEHIEEAGVHSGDSACVIPPQSLTPSQIERLRDYTRRI
                     ALGLGVVGLVNIQYAIKDDVVYVLEANPRASRTVPFVSKATGIPLAKVAAKVMMGRKI
                     RDLGYREREIDHVAVKEVLLPFNKLPGVDTVLGPEMKSTGEVMGIDYDFGRAYYKACI
                     SADNELPTSGKVFISISADQREEIAPIAKKLVGMGLTLYGTDGTVAFLHDVGVEAQMV
                     RKVQEGSPNVIDMIRNGEIRLIINTPRDKQSRQDHYQIMRTAVELGIPYITTTQAAKA
                     AANAIDAIRREKITIEPLGHYIGGVR"
     gene            4907..6109
                     /locus_tag="VKO45_08755"
     CDS             4907..6109
                     /locus_tag="VKO45_08755"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HMA05999.1"
                     /translation="MKKGTGKKGTVVLAFSGGLDTSVCIPLLREKYGYGRVITVAVDV
                     GQPESDIRDATEKGRRFADAHYTIDAKDRFVEEYVFPSLQANGSYEGYPLGTALARPL
                     IAGEAVAIARSSGAKAVAHGCTGKGNDQLRFDFVIRSSGLEVVAPIRELNLTRSWEIE
                     YARKHKIPVTVAKKNPYSVDENCWNRSIEGGRLEDPAFHPPESIYRWTVDPRKAPDSP
                     EEVTVRFEKGIPVALDGKRMKGIDLVQEMNLRAGRNGVGRNDMIEDRILGLKAREVYE
                     HPAATALSAAHADLEHLVLTRAELAFKRSVDDRWSDLAYQGLVHEPLFAALSAFIRKT
                     QERVNGIVDLVLYRGSVRVAGRSSPDAIYSGEMVSFDSMSLDQGHAEGVSRFHGIQAQ
                     ILKKKREG"
     gene            complement(6113..6517)
                     /locus_tag="VKO45_08760"
     CDS             complement(6113..6517)
                     /locus_tag="VKO45_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HMA06000.1"
                     /translation="MAKLSDDMKAVFSKNKIFPVATASKGGVPNVAPIAFVQIVSDDT
                     IWLGDNYMVKTLANVKENPWMAIEFWDPETKRCFQVKGKVAVKTSGAEYEKVKAMIKA
                     KNEKYPAKSLLILSITEVFECTPGASAGKKVA"
     gene            complement(6640..7542)
                     /locus_tag="VKO45_08765"
     CDS             complement(6640..7542)
                     /locus_tag="VKO45_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HMA06001.1"
                     /translation="MPPFDFDLSASIFSEGDPDIRSYRDGTFRQVLRLGGRLVLVDVT
                     AGGTVDRPQVGVEVRPGPIPPGIRKVAGETVSRLFNLDLDLSPFEREVGADPVMSAMS
                     QRLRGLKPPRTATVFEALIDSITEQQISLTAAHSIERRIVRTFGGTLEVEGHAYFAFP
                     SPERLADASLEELRICGLSGKKAEYINGIARRVREGTLDLENHEPGEETADIIRDLTA
                     IRGVGVWTAELAALRGLSRLDAIPADDLGIRRAISRYYSRESRIDTTEARRIAEAWGR
                     WRGLAAYYLIVAERRGIEVPDPGS"
     gene            complement(7690..8445)
                     /gene="budA"
                     /locus_tag="VKO45_08770"
     CDS             complement(7690..8445)
                     /gene="budA"
                     /locus_tag="VKO45_08770"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HMA06002.1"
                     /translation="MDTEAKKAILIVLGLALVFALGYVSGGLLPGHQEIRDQDLLYQV
                     STYTSLAGGGFDPVESVGTLMANGDLGIGTFTGLDGEMVVMGGTCYQVKSDGTVRPAD
                     SSQGVPYAEVTFFSPDLSVPGVMAGNMTQLTGFLDSRLPSRDQFYAIRMTGTFAYIRA
                     RSPPAQKKPYPLLADALKGQSIFEWRNVTGTAVGFYTPATAAGIGFPGYHLHFLSADR
                     TRGGHVLDLETTGNTVELDPTPRSLVIFTPEGA"
     gene            complement(8544..9740)
                     /locus_tag="VKO45_08775"
     CDS             complement(8544..9740)
                     /locus_tag="VKO45_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HMA06003.1"
                     /translation="MRLVGTAHVSAQSVEEVKKAIEEWHPDIVAVELDLGRFQALRKE
                     SHDPTVAEVLEVRNFNQLLIQWILSYLQRRIGIDVGVEPGAEMKAAIAEAEQRRIPVS
                     LVDRDIRLTLLRFWQSLGIIGKIKMFFALIISIAEVDEFEKVDIESMKDQSVIDMVME
                     EFRKFSPEGARSLIDERDAFIAHNLIRLSEQNIPRILAVVGAGHIHGIQGYLDNPSAL
                     PPMESLTRQPKPFPWATVFGFAVTALFLLLLAAIGFSGVGLGTLLYAFLFWVVIHGVL
                     AAAFTVAAGGHPFSALTCLGVAWMTSLNPLLHVGWIAAAVEAKVRRPQVADFRRIYEA
                     ETLTEMARIPLFKVVLIAALANLGSLLGTLLYFVFLFPVLGIDPVVVISTGLHNMAAW
                     VGGLVF"
BASE COUNT         1778 a         3175 c         3154 g         1640 t
ORIGIN      
        1 accactgcgg cggggccggg cttttcccgg cggtcgcaag gctcacccgg acctccctcg
       61 agtgcatccg ggcgagggac cgggccttcg ggtcccggag cgagggactc atcctcgaga
      121 ccgtgtatac caccctcatc aacctcttct cggggttcag gcccccgcgg gagctggaga
      181 tcctccctga gccgggcccg gccgtcacgg gagtctttcc cgtcatctcc cggttcaccc
      241 tctcgggtca tgccttcgag atcctgcagg gcctcaacgg gcacatggcc gggcaggtgt
      301 acctcgcttc acgggagctt ggagtcctct tcaccggcga tagcctgatc cacctggagg
      361 gcctcacccc ggagcgccgc tctttcaact ccctcgccgt gaacctcatc acctcggtga
      421 acgtggaccg ggagcttgcc cggcgggagc gggatgggct cctcgtcctc gcggcctcct
      481 gggaccggga tgtacccgcc ggagggaggc gctgcctcat ctgcggcggt cacgggccgg
      541 tgtcgatcct cgaggggagg tcccttgaca cctgcggtgc ggtggagcgc tgcaacccgt
      601 agatatatcc gtctcaaagg ggctccttcc gcatggcatc cgcatcgtta atacatccgg
      661 tccaccactc tgtatggtaa cttcatgaag gcggtcctgg gactcgaaga cggggattct
      721 gtcgtcgggg aaggcttcgg ggtagagggc tcctgcgccg gggagctggt cttctgcacg
      781 acgatgaccg gatacatgga ggctctcacc gacccctcct accacgggca gatcctcatg
      841 ttcacctacc cccttatcgg gaactacggg gttgacaagg ggaacttcca gagcgagggg
      901 gtgaaggccc tcgggtgcgt ggtccgcgag atctgccggg tcccttccgg cggaccccct
      961 ctcgaccgct acttcgagga gcagggcctc ctcgggatca ccggcgtcga tacccggcgc
     1021 ctcaccatca agtgccggga gatggggacc atgcgggccg ccctcgtcgt gggggatgac
     1081 aggagggagt acgcggtgga gtgcgcaaag aaggttcccc tcatcaccga ggtggacctt
     1141 gtcgccgcgg tctcgggcag ggagccccac cacatcccgg gcacgggcaa gcggatcgcg
     1201 gttctcgacc tcggggtcaa gcggaacatg ctcatctcca tgatgaagag gggcgccgac
     1261 ctgcacgtct tcccctacga cacccctcac gacgtgatcc aggcctgccg tcccgatgcc
     1321 ctcttcatca gcaacggccc cggggacccg aagagggtga ccgccgcggt ggagtgcgtc
     1381 cgggatttca tcggggagat ccccattttc gggatctgct tcgggaacca gatcacggcc
     1441 ctcgcgctgg gaggagatac ctacaagctg aaattcggcc accgtggcgt gaaccagccg
     1501 gtccggttcc atgacgggtc catcttcatc accacccaga atcatggctt ctgcgtggac
     1561 ggcgagagcc tccccgaggg ctgtaccgtg tcgtatacca atgtgaacga cgggaccctg
     1621 gagggattcg agaaccggga cctttccatc tcctgcgtcc agttccaccc cgaggcccac
     1681 ggggggccct tcgacaccga ggcgcattac ttcgatacca tgttccggag gattccctga
     1741 tgccccgtaa ggagcacatc aggaaggtcc tcctcatcgg ctccggcccc atccagatcg
     1801 ggcaggccgc ggagttcgac ttctcgggct cccaggcctg caaggccctc agggaggagg
     1861 gggtgacggt ggtcctcgtc aactcgaacc cggccaccat ccagaccgac cccgagatgg
     1921 ccgacaccat ctacatcgag cccctccggg cagagaccat cgcgaagatc atcgagaagg
     1981 agcggcccga cgggatcctc tccgggatgg gcgggcagac cggcctcaac atgaccgccg
     2041 agctcgccga gaaaggggcg ctcaaaggtg tcgagatcct gggcaccccc ctcgaggcca
     2101 tctaccgggg ggaggaccgg gagcagttca agaacctgat gcagtccatc ggggagccca
     2161 tcccccggtc catgatcctc cgggacatca gggagctcga ggaggccgag cgcctggtgg
     2221 gcctcccggc catcatccgg cccgcgtaca ccctcggggg gagcggcggc gggatcgccc
     2281 atacgaagga ggagctccgg aagatcaccg aggtcgggct cacccgctcc cgcatccacc
     2341 aggtcctcat cgaggagagc gtcctcggct ggaaggagat cgagttcgag gtgatgcggg
     2401 acgcggcgga cacctgcatc atcgtctgcg gcatggagaa cgtggatgcc atggggatcc
     2461 acacggggga gagcgtcgtc gtcgccccca tcctcaccct ccgggacgac gagttccaga
     2521 tgctccggac cgcggccatc aagatcatcc gggctctcgg agtccagggc ggctgcaaca
     2581 tccagttcgc ttacgagaag ggggactacc gggtcatcga ggtgaatccc cgggtctccc
     2641 ggtcctcggc cctggcgtcg aaggcgacgg gctaccccat cgcacgggtg gccgcaaaga
     2701 tcgccatcgg cctccacctg gacgagatca tgaacaccgt gaccggctgc accccggcct
     2761 ccttcgagcc cgccgtggac tacgtggtgg tgaaggtccc ccgctggccc ttcgacaagt
     2821 tcaagaccgc cgaccggacc ctcaccacgg ccatgaagtc cacgggggag gtcatgtcca
     2881 tcgggaggac cgtggaggag gccttcaaga aggccctccg ttccgtggac accgatgtga
     2941 cccggcacac gaacgcccac gagatccgga tgatcctcca gcggcccacc gacgagcgtt
     3001 tcggctgcct ctttgatgcc ttccgggagg gtttcaccgt ggaggaggtc tcggggctca
     3061 ccttcatcga cccctatttc ctcgagaagg tgaggaacgt cgtggagatg gagcgccggc
     3121 tctcgggggc ggcgagcccg gttctcatcc ggaaggccaa ggagttcggc ttctcgaacg
     3181 ccgagatcgt ggggctctcc ggcctcccct ggggagaggt ggagaacatc gccgggaaac
     3241 ccacctacaa gatggtggac acctgcgccg ccgagttccc ggccaccacc ccgtacttct
     3301 actcgacctg ggacaccgag tgcgagatca tcccgacgga gaaccggaag gtactcatcc
     3361 tcggctcggg gcccatccgg atcggccagg ggatcgagtt cgactactgc acggtccatg
     3421 ccgtgaaggc ggtgcgggac gagggggtcg aggtccacat cgtgaacaac aaccccgaga
     3481 cggtctccac cgacttcgat acctcggacc ggctcttctt cgagccgatg catctcgagg
     3541 acgtggtgaa catcctgaaa aaggactcct acgggggggt catggtccag ttcgggggcc
     3601 agaactccat caacctcgcc ctccccctgg agaaggagat cgcccggctc ggcatcccga
     3661 cgaagattct ggggacgagc cccgacgcca tggacatcgc cgaggaccgg gaccggttct
     3721 ccgaattcct ggaccggctc cggatcccga gcccgccgaa cagctccgcc tattcagagg
     3781 aggaggcctt ttccatcgcc gagagaatcg gctaccccgt cctggtccgg ccctcgtacg
     3841 tcctcggggg gcgggcgatg gagatcgtcc atgacagcgc cgacctcgcg acctacatca
     3901 aggaagcggt ccgggtctcc cggaagcatc cggtcctcat cgacctcttc ctgcagaacg
     3961 cggtggagat cgacgtggac gcggcctgcg acggcgagga cgtcctcatc ggcgggatca
     4021 tggagcacat cgaggaggcg ggggtccact ccggggactc ggcctgcgtc atcccgcccc
     4081 agtccctcac cccctcccag atcgagcgcc tcagggatta tacccggcgg atcgccctcg
     4141 ggcttggcgt cgtcgggctc gtgaacatcc agtatgccat caaggacgat gtggtctacg
     4201 tcctcgaggc aaacccccgg gcgagccgca cggttccctt cgtctcgaag gcgacgggca
     4261 ttcccctcgc caaggtcgcg gcgaaggtga tgatggggag gaagatccgg gacctcgggt
     4321 accgggagcg ggagatcgac catgtcgcgg tgaaggaggt gctcctgccc ttcaacaagc
     4381 tcccgggggt ggacacggtc ctcggcccgg agatgaagtc cacgggggag gtcatgggca
     4441 tcgattatga cttcggccgg gcctattaca aggcctgcat ctctgcggac aacgagctcc
     4501 ccacctcggg gaaggtcttc atctccatct cggccgacca gagggaggag atcgcgccca
     4561 tcgcgaagaa gctcgtgggg atgggcctca ccctctacgg gaccgacggg accgtggcct
     4621 tcctccacga cgtgggggtc gaggcccaga tggtgcggaa ggtccaggag ggctccccga
     4681 acgtcatcga catgatccgg aacggggaga tccggctcat catcaacacc ccgagggaca
     4741 agcagtcccg gcaggaccac taccagatca tgcggacggc cgtcgagctc gggattccct
     4801 acatcaccac cacgcaggcg gcaaaggccg cggcgaacgc catcgacgcc atcaggaggg
     4861 agaagatcac catcgaaccc ctggggcact atatcggagg agtcagatga agaagggaac
     4921 gggtaagaaa ggaacggtcg tgctcgcctt ctcgggcggc ctggacacct cggtctgcat
     4981 tcccctcctg cgggagaagt acggctacgg ccgggtcatc accgtcgccg ttgatgtcgg
     5041 gcagcccgag tcggacatcc gggacgccac cgagaagggg cggaggttcg ccgatgccca
     5101 ctacaccatc gatgcgaagg accggttcgt ggaggagtac gtcttcccct ccctccaggc
     5161 gaacggctcg tacgagggct acccgctggg gacggccctc gcccggcccc tcatcgccgg
     5221 ggaggccgtg gccatcgcaa ggtccagcgg ggcaaaggcg gtcgcccatg gctgcacggg
     5281 caaggggaac gaccagctcc ggttcgactt tgtcatccgg tcctcggggc tcgaggtcgt
     5341 cgcacccatc cgggagttga acctcacccg gagctgggag atcgagtacg cccggaagca
     5401 taagatcccg gtcacagtcg cgaagaagaa cccgtactcc gtggacgaga actgctggaa
     5461 ccggtccatc gagggagggc ggctcgagga ccccgccttc cacccgccgg agtccatcta
     5521 ccggtggacc gtcgacccga ggaaggcccc cgattcaccc gaggaggtga cggtcaggtt
     5581 cgagaagggg atcccggtgg ccctcgatgg aaagcggatg aagggaatcg atctcgtcca
     5641 ggagatgaac ctccgggccg ggaggaacgg cgtaggccgg aacgacatga tcgaggaccg
     5701 gatcctcggt ctcaaggccc gggaggtcta cgagcacccg gcagccaccg cgctttccgc
     5761 cgcccatgcc gaccttgaac acctggtcct cacccgggcg gagctcgcct tcaagcgttc
     5821 ggtggacgac cggtggtccg acctcgcgta ccaggggctc gtccacgagc ccctcttcgc
     5881 cgccctctcg gccttcatca ggaagaccca ggagcgggtg aacggcatcg tggatcttgt
     5941 cctctaccgg ggatcggtga gggtcgccgg caggtcatcg ccggatgcca tctactcggg
     6001 ggagatggtc tccttcgaca gcatgagcct cgaccagggt catgccgagg gcgtgtcgcg
     6061 gttccacggc atccaggcgc agatcctgaa aaagaagaga gagggatagg gctcaggcga
     6121 ccttcttccc cgccgaggcc ccgggggtgc actcgaagac ctcggtgatg ctgaggatga
     6181 gaagagattt ggccgggtac ttctcgttct tggcctttat catcgccttg accttctcgt
     6241 actcggcccc gctcgtcttc accgccacct ttcccttcac ctggaaacat ctcttcgttt
     6301 cgggatccca gaactcgatg gccatccagg gattctcctt cacgtttgcc agggtcttca
     6361 ccatgtagtt gtccccgagc cagatggtgt cgtcggagac gatctggaca aaggcgatgg
     6421 gggcgacgtt cgggaccccg cccttcgagg ccgttgccac ggggaagatc ttgttcttcg
     6481 aaaacaccgc cttcatgtca tcggatagtt tcgccatgcc gtccacctca agaatctcgc
     6541 gaaacctgga atacgggttc cgcggggtaa tatgccctgt tccctgtttc tatttatgga
     6601 tattggatgg cccgcactct tccccggcag accccatcat caggagcccg ggtccgggac
     6661 ctcgatcccc cgtcgttccg cgacgatcag gtaataggcg gcaagacccc tccatctccc
     6721 ccatgcctct gcgatccggc gggcctcggt ggtatcgatg cgggattccc gcgaataata
     6781 ccgggagatg gccctcctga tgccgagatc gtcagcgggg atggcgtcca gccgggagag
     6841 tcccctgagg gccgcgagct ccgcggtcca cacccccacc ccccggatgg cggtgagatc
     6901 ccgaatgatg tcggcggttt cctcgcccgg ttcatggttc tcgagatcca gggttccctc
     6961 cctgacccgc cgggcaatcc cgttaatgta ctccgccttc ttcccggaga gcccgcagat
     7021 cctgagctcc tcaagggatg catcggcgag ccgttccggt gaggggaagg cgaaatacgc
     7081 gtgcccctcc acctccaggg tgccgccgaa ggtacggacg atccgcctct cgatgctgtg
     7141 ggctgcggta agagagatct gctgctcggt aatggagtcg atgagggcct caaaaacggt
     7201 ggcggtcctc gggggtttca gtccccggag cctttgtgac atggccgaca tgaccgggtc
     7261 tgccccgacc tcccgctcga agggggagag atccaggtcg aggttaaaga gccggcttac
     7321 cgtttctcct gcgaccttcc ggattccggg cgggatgggt ccgggcctga cctcaacccc
     7381 cacctgtggc cggtccacgg ttcccccggc ggtgacgtcg acgaggacca gccggcctcc
     7441 cagcctgaga acctggcgaa aggtgccatc ccggtaagat ctgatgtcgg gatcgccctc
     7501 cgaaaagatg gatgccgaaa gatcgaagtc aaacggtggc acgggagaga ggcggagaat
     7561 ctcgtgggtt gatcctccaa tgcctccggc gcggttcccc cggtccccgg ttcccggctt
     7621 catgctcctc tcttcagggc ccgaaccgac ggccggtgga tgggatgaaa ggcatgcgga
     7681 gccctgggat caggccccct ccggggtgaa gatgacaagc gatcggggtg tcgggtccag
     7741 ctccaccgtg ttccccgtgg tctcgaggtc gaggacatgg cctccccggg tccggtcggc
     7801 agagaggaag tggaggtggt agccgggaaa accgatcccc gcggcggtgg ctggggtgta
     7861 gaacccgacc gccgtccccg tgacgtttct ccactcgaag atggactggc ccttcagggc
     7921 gtcggcgagc agggggtagg gtttcttctg ggcaggcggg cttctcgccc tgatatacgc
     7981 aaaggttccc gtcatccgga tggcatagaa ctggtccctg gaggggagcc gggagtcgag
     8041 gaaccccgtg agctgcgtca tgttccccgc catgactccc gggaccgaga gatcggggga
     8101 gaagaaggtc acctcggcat aggggacccc ctgggaggaa tccgccggcc ggacggtccc
     8161 gtcgctcttc acctggtagc aggtcccccc catgacgacc atctccccgt cgaggcccgt
     8221 gaaggtgccg atccccaggt cgccgtttgc catgagagtt ccgacggact ccaccgggtc
     8281 gaatccccct cccgcgagcg aggtgtaggt cgagacctgg tagaggagat cctgatcccg
     8341 tatctcctgg tgaccgggaa ggagtccccc cgagacatat cccagggcga agaccagggc
     8401 gagccccagg acaatcagga ttgccttctt tgcctccgta tccatgcctc ctgaaacagt
     8461 gaggagttgg tccccctgat gataaaaata cggatccgga cattccatgc tccccccgcc
     8521 ctatgcggac gagccatggc ccttcagaag acgagcccgc cgacccatgc cgccatgttg
     8581 tggaggcccg tcgagatcac gaccaccggg tcgatcccga ggacggggaa caggaagacg
     8641 aagtagagga gcgtgccgag caggctcccc aggttcgcga gggcggcgat gagcaccacc
     8701 ttgaagagcg ggatccgggc catctcggtg agggtctcgg cctcgtagat ccgacggaag
     8761 tccgcgacct ggggcctcct caccttcgcc tccacggcgg cggcgatcca cccgacgtgg
     8821 aggagggggt ttaaggaggt catccaggcc accccgaggc aggtgagggc cgagaacggg
     8881 tgcccccccg cagcgacggt gaaggccgcg gcaaggacgc catggatgac cacccagaag
     8941 aggaaagcgt agagaagtgt gcccagccca acgcccgaga acccgatggc cgcgaggagg
     9001 aggagaaaga gggctgtcac ggcgaacccg aagaccgtcg cccaggggaa gggcttcggc
     9061 tgccgggtga gggactccat gggcggaagg gccgaggggt tgtccaggta cccctggatc
     9121 ccgtggatat gccccgcacc caccaccgca aggatccggg ggatgttctg ctccgagagc
     9181 cggatcaggt tgtgggcgat gaaggcgtcc ctctcgtcga tgagggacct cgcaccctcg
     9241 ggggagaact tcctgaactc ctccatcacc atgtcgatga ccgactggtc cttcatgctc
     9301 tcgatgtcca ccttctcgaa ctcgtccacc tcggcgatgg agatgatgag ggcgaagaac
     9361 atcttgatct tcccgatgat gccgagggac tgccagaacc ggaggagggt gagccggatg
     9421 tcccggtcca cgagggagac ggggatcctc cgctgctccg cctcggcgat ggccgccttc
     9481 atctcggccc ccggctccac cccgacatcg atcccgatcc tccgctggag gtaggagagg
     9541 atccactgga tcaggagctg gttgaagttc ctgacctcga gcacctcggc cacggtgggg
     9601 tcatgggact ctttcctgag cgcctggaac cggccgaggt ccagctccac ggcgacgatg
     9661 tccgggtgcc actcctcgat ggcctttttc acctcctcga cactctgggc cgatacgtga
     9721 gcggtaccga cgagacggat ctcgggc
//
LOCUS       DATGDI010000235        22038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_433437, whole genome shotgun sequence.
ACCESSION   DATGDI010000235 DATGDI010000000
VERSION     DATGDI010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 22038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22038
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433437"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VKO45_08780"
     CDS             <1..339
                     /locus_tag="VKO45_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06004.1"
                     /translation="WGIAELLLNPSGDSILPTVLLAAGLAFLVTGVSRWKKFGNTAEQ
                     DERSRRIGAWGMSYSWLLSISFMWVLFLLDHFRVVMLATGTAIGVSLVVMALSAVAFQ
                     MYLFRKGDVE"
     gene            364..579
                     /locus_tag="VKO45_08785"
     CDS             364..579
                     /locus_tag="VKO45_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMA06005.1"
                     /translation="MQTRIKEYRNSLGLTQEELAGKVQVRRETIVFLEKGKYNPSLRL
                     AHSVARALGTTVDGLFSFEDEESGAGG"
     gene            680..2092
                     /locus_tag="VKO45_08790"
     CDS             680..2092
                     /locus_tag="VKO45_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HMA06006.1"
                     /translation="MEPEMREFLEKGLVSAARMRAVEGNAVTLGVGSLQMMESAGKAL
                     AAMALEGSPSRVLVLCGRGKNGGDGMVAARYLQHLETHLIYASDGPFAHETEVQLRAL
                     AGSAVSLHPVKCASDVELLSPLFSGVEVIVDALLGTGASGVTREPVSRCVELASASAA
                     PIIAADIPTPGMRATRILSFHRAKTEGAGVADIGIPLAAEVFMGPGDLTLVPRRGPKA
                     RKGENGEVLVIGGGPYQGAPWLAGLGALRAGADIVRIASPAFEPVPDLIYERLEGERI
                     TTGHLERLLPLVERADSVVVGNGLGTESHDVVLAVAEAARKAVYDADALRLPLPRPMP
                     VAGEAVYTPHAGEFERMTGRRPPEDLVPRGRAVRDAAKEGVVVLKGPVDVISDGTRVR
                     FNPTGSPAMAVAGTGDVLAGVTGALLCRLPAFEAACIAAYVNGSAGMLAAQRLGDGMT
                     ASDLVNEIPAVLFAGRTGNG"
     gene            2085..2549
                     /gene="moaC"
                     /locus_tag="VKO45_08795"
     CDS             2085..2549
                     /gene="moaC"
                     /locus_tag="VKO45_08795"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HMA06007.1"
                     /translation="MAEFTHVKGDRVQMVDISGKDEVAREAVAEGKIRLRGETLRAIR
                     EGTVIKGNVLATARVAAILAVKETPRIIPLCHPIPLSGIEVDFADGEGFIRATVRVRS
                     RGPTGVEMEALTGVSVALLTIWDMVKSAEKDSAGQYPETGIDGIRVLSKKKG"
     gene            2656..3246
                     /locus_tag="VKO45_08800"
     CDS             2656..3246
                     /locus_tag="VKO45_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HMA06008.1"
                     /translation="MVRSMYSYVREAWKHADTSGVSDLLWERMQQWRDEGTVVRVDRP
                     TRIDRAHALGYKAKQGVAVARVKVHRGGRRASRYVRGRRTAHMGMRKKTPAKSLQRIA
                     EEKASRKFPNMEVLNSYWVGEDGRHRWYEVILVDGHHPSIRRDPQLSWAGERGHRGRA
                     ERGLTSAGKKGRGMRRRGIGTEKNRPSIRSHANRGK"
     gene            3267..3902
                     /locus_tag="VKO45_08805"
     CDS             3267..3902
                     /locus_tag="VKO45_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P subunit p30 family protein"
                     /protein_id="HMA06009.1"
                     /translation="MPLIDGCIHPYPAGDSSLRRMALEARELGFDQVVAAGGTGEVPG
                     VLRGNIISASSVREVVSRLQGIARGEGVVMVNSGDLAFNRGVIGLRGVRVLRGIHLSA
                     RGAFNYVAAREAEAKGVAVELDLSPLIGDRGIRRQRAISRYRDLLDLHRRYHFPLTLA
                     SNARSILGQRSVRDIVLLARLFGMEEAEVTGALSSLDSILAGRSPVQVVRT"
     gene            3899..4375
                     /locus_tag="VKO45_08810"
     CDS             3899..4375
                     /locus_tag="VKO45_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpp14/Pop5 family protein"
                     /protein_id="HMA06010.1"
                     /translation="MSELPSHRERRRYLLARFVPEGASPDPREVYAAVVEACTSLWGD
                     VAASEISPSVMAWEGHAILRCRRGTEDRLSTALATITKVGDVRVAMRSLATSGTLHAI
                     RSRANPKPGILWEKQVGIGGRTFLARAYPGQKVDLIEKGIKGQELLFFADEDGEVR"
     gene            4375..5109
                     /gene="psmA"
                     /locus_tag="VKO45_08815"
     CDS             4375..5109
                     /gene="psmA"
                     /locus_tag="VKO45_08815"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HMA06011.1"
                     /translation="MQPQYQMGYDRAITVFSPDGRLYQVEYAREAVKRGTTAVGIKCT
                     DGVVLIVDKRVATRLLEPSSIEKIFKIDEHIGVASSGLVGDARALVDRARVECQINRV
                     SYNEAIDVETLAKKLCDHMQTYTMFGGARPYGTALLIAGVSDGTTRLFETDPSGTLLE
                     YKATGIGTGRPAVMKVFEEEYNPEAGCKEAILLGLKALHAATEGKFDVNTVEIGIVEE
                     KAGAFRKMTRDEVASFVEQFEKTGAA"
     gene            5106..5798
                     /locus_tag="VKO45_08820"
     CDS             5106..5798
                     /locus_tag="VKO45_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HMA06012.1"
                     /translation="MIPLDRAVVARLETHGERFEILVDPDLAVKIRHGEPVDIEDAVA
                     AIFIFENASRAEKSSDESLQKVFQTTDFSTIARRIIEKGEIHLTAEQRKELLAEKRGQ
                     VITFIARHAINPQTGLPHPPARIEMAMEEAKVSIDPFRPLDDLVADTMKALRPLIPIR
                     FEEVRVAVKIPAEHAARAYGDIDSFARIEQEEWQKDGSWICVVKIPGGMQQELENLVR
                     KITKGTGQTRVI"
     gene            5868..6158
                     /locus_tag="VKO45_08825"
     CDS             5868..6158
                     /locus_tag="VKO45_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37ae"
                     /protein_id="HMA06013.1"
                     /translation="MSTTKQGSKGRVSRSAGRFGPRYGRFIRKRVVEVEDISRATHTC
                     PSCDQVAVRRKGTGIWQCRKCGYKFAGGAYVPQTPTFRIAQRSIEKPQVPEE"
     gene            6166..6300
                     /locus_tag="VKO45_08830"
     CDS             6166..6300
                     /locus_tag="VKO45_08830"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HMA06014.1"
                     /translation="MPGVYKCARCKHKVEIDVNVRCPYCGHRILFKERGAGIKDLKAR
                     "
     gene            6297..6701
                     /locus_tag="VKO45_08835"
     CDS             6297..6701
                     /locus_tag="VKO45_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06015.1"
                     /translation="MITVTTSRRADPLVRRIGRDFAFATGGTYVTRGKGGLSRPPFSG
                     GIVLVLSRDGKGIRTQILSGSEDAAILRFPEVREESRAGPLRKGLFTGDRELFSTLSP
                     LLPVSLEGDLPDTVVLDGIQGRRISLLVPGEA"
     gene            6698..6964
                     /locus_tag="VKO45_08840"
     CDS             6698..6964
                     /locus_tag="VKO45_08840"
                     /inference="COORDINATES: protein motif:HMM:NF011470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HMA06016.1"
                     /translation="MRHTASFSFRSDRAGEIFQALAPERGEINPRTKARVTLEQGDIL
                     VVSIAAGDIPALRAALNMWLRLVSVAGEMLEMAQAKGKEQEGFG"
     gene            6978..9203
                     /locus_tag="VKO45_08845"
     CDS             6978..9203
                     /locus_tag="VKO45_08845"
                     /inference="COORDINATES: protein motif:HMM:NF021416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2117 domain-containing protein"
                     /protein_id="HMA06017.1"
                     /translation="MPSGRGQRGRRRTGPGRFADAVMVVHGPEAFDRGDVALLQDLLG
                     PGETVVAGVMARTAAEESGLPVIIDGGIPSSVIGRIPGPEGNRDRPGGKRAPLERIPG
                     PEGRPGAEGAGAVSGRRRREAAPPVQGGRGPSGPGKGAPLDRTERRVFLVNRGKTPES
                     GRIFGEIVASRLPRGRPLVQLECSSRTVFLWNGSGRSREFARLLAGETGFSFTETTAG
                     REEESAEREIRGCIPGEAVLVEGIVIGTATAGTVVLRKSEGRIRVVSGLRPKAHGLEK
                     LSRLGDIDLGRAWCKSGRIRGVPPRAGGPAPAAGRVVVIDHRGHDLYRFLTPDTCGVL
                     AIGDDTTSVCGHICSHLGIPVFGVVDGDRDDLLVSSFPPGSVIAEVSGGSDDEAGREL
                     AGHNQDEIIAWEDWVARALAILGNRCRVIHRSPPRRRNRRKRKADPGPETPTAPSPGR
                     MNLINLCRKCHSMERSRFLPLLLPLLAALGAAQETSALPEEGIRLLDFAFLYYLPVLA
                     GLLLVFLVFTGANRVLRRRYSVVEFVGTAAGFLLSLLFIATGFLFLASAVVLAWATAI
                     GDITYAAAEEWAVPISNEVQYLLQPALDLLSGTADYTPYLQDILLGVLIAGGAALIII
                     GVILIWKLPKTSFYEIIKTSEDTTFKEVMTRGREPLTPTVTFRVIDRLTNQPSPDVKV
                     ILKHKEGAKVYNRYTDFNGEVIFQKIDGLYSQYYAYVEGDEERKRYWVIRTAIGAETV
                     T"
     gene            complement(9166..10803)
                     /locus_tag="VKO45_08850"
     CDS             complement(9166..10803)
                     /locus_tag="VKO45_08850"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HMA06018.1"
                     /translation="MLFREFAEICERLEAISGRLEMIDLLAQDLPRLDDRELPVFVRF
                     IMGRIFPDWSPLKLGIGPNLLYEAVAYVVGKKREAVETKVSAEGDVGMAVELLLVGKT
                     QTSFFSEEMTIGDVSAACEAIALSEGKKSQREKLLAVRKLFASVQPLEGRYLARLILG
                     ELRIGIGEGNVRDAIAKAFSLEPGEVDHAYQAMNDLGEVARLARQGREALSAVRIEIF
                     RPVKMMLAQQGSISDMVSEHGKVAAEYKYDGTRIQFHKKGNEVRVYSRKLEEVTKAIP
                     DVVTRLLAATKRDVILDGEAVAMKGGRPMPFQFVLRRFRRKYDIEEQAGKIELVPYIF
                     DILVLDGETLIDLPFRERRKHLEEALKDHIAPQLVSDEPAALEKFYNEALDAGQEGIM
                     VKDLDSRYQPGVRGKHWIKVKPEVDTLDLAVIGGEWGEGRRAHFFGSFLLACQDGGRL
                     LPVGKVATGITDEMLATIYEVMKDLVVSEKGKEITFEPQVVFEVGYSEIQTSPNYASG
                     FALRFPRFIRIRDDKGVDEIETLESIRSRFRRQSPSG"
     gene            complement(11123..11896)
                     /locus_tag="VKO45_08855"
     CDS             complement(11123..11896)
                     /locus_tag="VKO45_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA06019.1"
                     /translation="MEITVLASGSKGNCILASGEDGALLVDCGIPAREVLARLTAAGK
                     DPDPIGGILLTHEHIDHVRGVWALARKLKVPICGTEGTLLELPREASRGHCPSLHTVH
                     PSEPFELGGFTLEAFPTSHDAREPCGYYVGEKGCRFAFCTDTGFLGPSLVSGLSRCDG
                     VLLESNHCPEMLRTGPYPESLKRRIRSRRGHLSNVAAATCLREIAGDVGAVILAHLSE
                     INNTPEKALASAREGLAFYLGDVEILVARQHVFTPTLTL"
     gene            complement(11991..13070)
                     /locus_tag="VKO45_08860"
     CDS             complement(11991..13070)
                     /locus_tag="VKO45_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA06020.1"
                     /translation="MDAIEAQGLSKSYGEKRILREITLSIPGGRIFGLIGPSGAGKST
                     LLRLLDLLEPPTGGRLSILGEDALPPSSRFQLRRRMGMLLQKPVIFNRTVHENIAIGL
                     KYRHESRDRIESRVRGVLHDVDLPGYSPRPARTLSGGEIQRVALARALVTDPEILFLD
                     EPTANLDPPSAENLEEIILRLNREKGMTLVMSTHDMLQGQRLAGSIGVLMGGTLTQVG
                     NSLEIFQRPASKDIARFVKVGNILSGLVTENREGEVIVDVSGKVFRAVATLQPGTRAI
                     LLFRSEDVTIDLAEGVKSSARNVFPGRIKRVVALRPVVQVVIDCGIEIMALVTASSAE
                     DLGLSEGREVRVSVKATAIHVLPET"
     gene            complement(13085..13801)
                     /locus_tag="VKO45_08865"
     CDS             complement(13085..13801)
                     /locus_tag="VKO45_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA06021.1"
                     /translation="MAGEIADGFIQAIQLIVTGNPEVMRITLLSLEISLAATILSASI
                     SIPAGALLYYHEFFGKRVLVNILQTLYALPTVLAGLLLFLLISHQGPLGFLSLLYTPY
                     GMVIAQMILVLPLITGLTFSTLSGIDPAMRSTVLSLGATKIQSIGTLILETRFGILAA
                     VILGFGRAISEVGAAMIIGGNIRGYTRVLTTAISLETSIGDFALAIALGIVLLGVALL
                     VNIALNLIQSGSVLKNTWIS"
     gene            13946..14938
                     /locus_tag="VKO45_08870"
     CDS             13946..14938
                     /locus_tag="VKO45_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HMA06022.1"
                     /translation="MKSTRKHCLGCLLGIVILTAILLSGCTQPQTVPANPTPSATTVA
                     ATPTPVVHSPNTLLIATTTSLYDTGLLNAVQDLYENQTGIHLKITSQGTGIAIQVATR
                     GDCDLLLVHSPSQEKAFIDKGLGVNQRCFAYNYFEIVGPASDPAGIANLTPEAAFTKI
                     RTLGLNNTPGVFFASRGDQSGTHSAEQNIWKGAGFNYTRDVATSGSWYLSLGKGMGET
                     LTVAGQKGAYTLTDEGTFLAFKSQLNLVPLITKGTSLLNRYSAIAVNHSVNSNVNIVQ
                     ADRFINWLVSDEGRQFVGNYGVEKYGKSLFTPLTPDGCTKAPFNCTCSGDVSPI"
     gene            15048..16286
                     /gene="hisD"
                     /locus_tag="VKO45_08875"
     CDS             15048..16286
                     /gene="hisD"
                     /locus_tag="VKO45_08875"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HMA06023.1"
                     /translation="MWKELEVGAWVASRKQSMEDVRAQVQEIIGRVRAEGDTALRALS
                     RHVKLTEIAVSDEEREAAYSEVDPAVTEALVEAEARITRFHELQRPRDLWLQEVEPGV
                     VLGMKTTPLDRIGIYVPGGRAAYPSSALMCAVPARVAGVREICACSPPPVSPMTLVAL
                     DIAGVREIYRSGGAQAVAAMALGTETIPQVQKIVGPGNVFVTMAKVLLREEAEIDFPA
                     GPSEIAIIADDSADPRILAWDILAQAEHDPRAACVLITPDRALAEKVGKQVAGLAGKE
                     PRKEILAESLENCGYVLVKDLDEAVAASDGIAPEHLSIQVKDPLPVLSRVRHAGAIFV
                     GRYSAVACGDYAVGTNHVLPTAGYARLYSGLDVNHFCKTSSVEILSREGLEAIGDAVE
                     TIATAEGLHAHAASIRARRE"
     gene            16297..17004
                     /locus_tag="VKO45_08880"
     CDS             16297..17004
                     /locus_tag="VKO45_08880"
                     /inference="COORDINATES: protein motif:HMM:NF016382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HMA06024.1"
                     /translation="MGGRVPFRLDAPGSLEEARAIQEAVAREVEAASPVSIDGVMRIA
                     GADASYSPDGRTVHAAVAVLGLPDLNLLERCWVSREVPFPYVPGYFAFREGPAVMAAV
                     SGLTRRPDLLLVDGHGRAHPRRAGIACHVGLLLGIPTVGVAKRILSGDAGEIGPARGS
                     RYAIMDRGEITGMAVRTKAGSRPVFVSAGFATDLETSVAAVISTTASSRVPAPVREAH
                     RISREVRWSSLPPSFRP"
     gene            complement(17042..19204)
                     /locus_tag="VKO45_08885"
     CDS             complement(17042..19204)
                     /locus_tag="VKO45_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMA06025.1"
                     /translation="MVQVVSPGIPTIPLRIMTPKDPDLPTGDDDTSSLAGTPGKTRSR
                     PEGSQHREAPGSAFGAPETGTPDAACSEPALYARVSSLLEEGRLREAEHLLTRGLEIY
                     PESVDLLKEFGVLYHLQGRFGKAARTFTRVMNITGEGRQSLSWRIASLHHQALDELGG
                     ADPGLSLPTFGRILALDPSNREARAGQIAALRLLGRLGEAGRYLQEGLSLDPPGASIL
                     YQEGWLCMDLDRPDLASGAFEKAAIADPAWPEPVLSKALAMERLGRGTEAGKMLRRFV
                     ESQGGAPHLRADLGWFLLSIRDLKGAREIFLELADREDDLQGFHGLAALMLAAGRMGD
                     ARGIMERLAGARPRDPLILVNTGMVLARSGKARDLADAAIAVKRALSLFPACGPALSC
                     LGIIASRQGKPDEAQAHFAGAVRQSDPAGYRNLGLLACARGRWGQAEPLLTRATGLDP
                     LDARAWAGLGAIALQAGRAGEALSHLRRAALLDPRDSAIARGLAIALARSGDPAGSEE
                     VIRRALGLTSGPERWVLLLELAALLVSRQGPNGDPVLAEEAVQLLAKAGQLRPDEPGI
                     LFYLGAAKARLGNLKEALELFASSMRGDRYRVPAQENGRRLKRYLSSRGRFPHGLPVR
                     VALAALFLLQLAGLWFLFAARLLSEAGFVFLIAILSGLLAVASLLPARNGGRMKEAPP
                     ELVLPERNFVPFPEADMVSPLIRLRTALRPRTDSRMEQ"
     gene            19308..19400
                     /locus_tag="VKO45_08890"
     CDS             19308..19400
                     /locus_tag="VKO45_08890"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HMA06026.1"
                     /translation="MKSEFRGKTYYFCAPGCKKKFDADPAKYAT"
     gene            19491..19949
                     /locus_tag="VKO45_08895"
     CDS             19491..19949
                     /locus_tag="VKO45_08895"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HMA06027.1"
                     /translation="MSYFEEIRKRGRVVNPFFVMMGIDVDSFGEGTARLSMRVRPDMH
                     NGVGWMQGGIFTAMADEAMALALYSVVLPGEGIATISETTSFLKGARKDDLLVATGRV
                     VRKGRRVAFTESEVRRDSDAGEVLARCAAAFAIVEKPDPASSGHGSRKKR"
     gene            complement(19975..>22038)
                     /locus_tag="VKO45_08900"
     CDS             complement(19975..>22038)
                     /locus_tag="VKO45_08900"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HMA06028.1"
                     /translation="EQTVTVVAGETAQADFVLTPVPTTGSIHVTSNPSGAEIWIDNAD
                     SGQTTEFTIDGITPGDHVVNVTLAGYEMAAEQTVTVVAGETAQADFVLTPVPTTGSIH
                     VTSTPSGAEIFIDNVDSGQTTEFTIDGIAPGDHVVNVTLAGYESAAEQTVTVVAGETA
                     QADFQLVPLPTTGSIHVTSNPSGAEIFIDNVDSGQTTEFTIDGITPGDHVVNVTLAGY
                     EMAAEQTVTVVAGETAQADFVLTPVPTTGSIHVTSTPSGAEIFVDNVDTGKTTESTID
                     GIAPGDHVVNVTLAGYELAAEQTVTVVAGETVDADFTLVPIPTTGSIHVTSTPSGAKI
                     FIDDGDTGMVTEATISDLAPGFHVAKVTMAGYEDAEQLVTVVAGETIDVNFQLVAIPS
                     GPKAQFLAFPRQGTAPLTVLFIDVSQGSPSTRLWDFGDGSTSTEMFPLHTFNAAGKYT
                     VRLTVKNKGGSDTMTREDFITVNGANPPKAQFIAYPRQGTAPMTVLFLDVSQGSPSSR
                     LWDFGDGTTSTDMFPLHTYVAPGKYTVKLTVTNSGGSDTLVRENFIIVRVGNPPKAQF
                     TAYPQDGAAPLAVTFLDLSRGYPSSRVWDFGDGASSTDINPVHVYEKPGKYTVTLTVT
                     NDAGSDTVVRHNFITVDGKKQKVMGENESGQQENESGGRGKEPTGAGYTPLPVTTQRP
                     QIKIG"
BASE COUNT         3869 a         7001 c         7416 g         3752 t
ORIGIN      
        1 tggggcattg ccgagctcct cctgaatcca tcaggtgaca gcatccttcc gaccgttctc
       61 ctggcggccg ggctggcctt cctcgtcacc ggagtgagcc ggtggaaaaa gttcgggaac
      121 accgccgagc aggatgagcg ctcgagaaga atcggggcat gggggatgag ttactcctgg
      181 ctcctctcca tctccttcat gtgggtcctc ttcctgctgg accatttcag ggtggtgatg
      241 ctcgcgaccg ggaccgccat cggcgtgtcc ctggtcgtga tggcactgtc ggcggttgcc
      301 ttccagatgt accttttcag gaagggagat gtggaataac catcgacacc cggggaggct
      361 ttcatgcaga caaggataaa ggaataccgg aacagcctcg ggctcaccca ggaggaactc
      421 gccgggaagg tgcaggtccg acgggagacc atcgtcttcc tggagaaggg aaaatacaac
      481 ccctccctgc ggctcgccca ctccgttgca cgagccctgg gaacgacggt ggacggtctc
      541 ttctccttcg aggacgagga gagcggggcc gggggatagc ccgggtccat ccccgcccag
      601 ggcctgcacg gactgattat ggtgcgaggg gacacttttt tccgtccctc tgccccatgc
      661 tggataaagg ggagctggaa tggaaccgga gatgcgggag ttcctggaaa agggtcttgt
      721 gagcgcggcg cggatgcggg cggtggaggg gaacgccgtc acccttggag tgggatccct
      781 ccagatgatg gagagcgcgg gaaaggccct cgccgccatg gccctcgaag gctcaccatc
      841 gagggtgctc gtcctttgcg ggaggggaaa aaacgggggt gacgggatgg tcgccgcgcg
      901 ctacctccag catctcgaaa cccatctcat ctacgcctcg gacggtccct ttgcccatga
      961 gacggaggtg cagctccgcg cactcgccgg ttcggcggta tctcttcacc cggtgaagtg
     1021 cgcatccgac gtggagctcc tctcccccct cttttccggg gtggaggtca tcgtcgacgc
     1081 acttctcgga accggggcat cgggggtgac ccgggaacct gtctcccgct gcgtggaact
     1141 ggcgagcgcc tccgccgctc ccatcatcgc cgcggacatc cccaccccgg ggatgcgggc
     1201 cacccggatc ctgagcttcc accgggcaaa gaccgaggga gccggggttg cggacatcgg
     1261 catccccctc gcggccgagg tgttcatggg gcccggcgac ctcaccctgg tcccgaggag
     1321 gggaccgaag gcccggaagg gggagaacgg cgaggtgctg gtcatcggcg gcgggcccta
     1381 ccagggcgcc ccctggctcg ccgggctcgg ggctctccgg gccggcgccg atatcgtgcg
     1441 gatcgcatca cccgccttcg aaccggtacc tgacctcatc tacgagcgcc tggaggggga
     1501 acggatcacc acaggccacc tcgaacggct tctcccgctc gtggagaggg ccgactcggt
     1561 ggtggtgggg aacggtctcg gaacggagag ccatgacgtg gttctcgctg tcgccgaggc
     1621 cgcgagaaag gcagtgtacg atgccgatgc ccttcgcctg cccctgcccc ggccgatgcc
     1681 cgtggcggga gaggccgtgt acacacctca tgcaggggag ttcgagcgca tgaccggccg
     1741 gagaccgccg gaggaccttg ttccccgggg ccgggcggtc cgcgacgcgg cgaaggaagg
     1801 cgtggtcgtt ctcaagggcc cggtggacgt aatctccgac gggacccggg tgcggttcaa
     1861 cccgaccggc tcgcccgcca tggcggtagc cgggacgggg gatgtcctcg ccggggtcac
     1921 cggggctctc ctctgccggc tgcctgcctt cgaggcagcc tgcatcgccg cctacgtgaa
     1981 cggctcggcg gggatgctcg cggcccagcg cctgggagac ggaatgaccg cgagcgatct
     2041 cgtgaacgag atacccgcgg tcctctttgc agggaggaca gggaatggct gagtttaccc
     2101 acgtgaaggg ggacagggtg cagatggtgg acatctcggg aaaggatgag gtggcccggg
     2161 aggcggtggc cgaggggaag attcgcctcc ggggtgagac cctgcgcgcc atccgcgagg
     2221 ggaccgtgat caagggaaat gtcctcgcca ccgcgcgggt cgccgccatc ctcgcggtga
     2281 aggagacccc gcgcatcatc cccctctgcc atcccatccc gctttcgggg atcgaggtgg
     2341 atttcgcaga tggcgagggg ttcatccggg cgacggtccg ggtccggtcc cggggtccga
     2401 ccggcgtgga gatggaggcc ctcacggggg tatcggtggc cctcctcacc atctgggata
     2461 tggtcaagtc cgcggagaag gactcggcgg gccagtaccc ggagaccggg atcgacggga
     2521 tccgggtcct ctcgaagaaa aaggggtagg gacagaggct tttataccat cccggcagat
     2581 ctatacagaa ccacgggata cgtccctggg aaaggaatgt ggccgttacc ggctgaacct
     2641 tgaggtgagg aaaccatggt acgatcgatg tactcctacg tacgggaggc gtggaagcat
     2701 gccgatacat ccggtgtctc ggacctcctc tgggagcgga tgcagcagtg gcgcgatgag
     2761 gggaccgtcg tcagggtgga ccggcccacc cggatcgacc gcgcccatgc cctcgggtac
     2821 aaggcgaagc agggcgtcgc cgtggcccgg gtgaaggtcc accgcggcgg ccggcgggcg
     2881 agccggtacg tcaggggccg ccggacagcc cacatgggga tgcggaagaa gaccccggct
     2941 aagagtctcc agcggatcgc cgaggagaag gcctcccgga agttcccgaa catggaggta
     3001 ctcaactcct actgggtggg cgaggacggc cggcaccggt ggtacgaggt gatcctcgtg
     3061 gacggccacc acccctccat ccggagggac ccgcagctct cctgggccgg agaacgcgga
     3121 caccggggcc gggcggaacg cggcctcacg agcgccggta agaagggccg cggcatgcgg
     3181 aggcggggca tcggcaccga gaagaaccgg ccgagcatcc gctcccacgc aaaccgcggg
     3241 aaataaccct attttttccg acccctatgc ctctcatcga cgggtgcatc cacccctacc
     3301 cggcaggaga ctcctccctc cggcgtatgg ccctcgaggc ccgggagctc gggtttgacc
     3361 aggtggtcgc cgccggggga accggggagg ttcccggtgt actccgtggg aacatcatca
     3421 gcgcatcctc ggtgagggag gtcgtctccc ggctccaggg aatcgcgcgg ggggagggcg
     3481 tggtgatggt aaactcgggg gatctcgcct tcaaccgggg ggtcatcggg ctccggggag
     3541 tgcgggtact ccggggtatc cacctgtccg cacggggggc cttcaactac gtcgcggccc
     3601 gcgaggcgga ggcgaagggg gtcgccgtgg agctcgacct ctcgcccctc atcggagacc
     3661 ggggcatcag gcggcagagg gcgatctccc ggtacaggga cctcctcgac ctccaccggc
     3721 ggtaccactt ccccctcacc ctcgcgtcca acgcccggtc catcctggga cagcggtcgg
     3781 tgcgggacat cgtcctcctc gcccggctct tcggcatgga ggaggccgag gtgacgggcg
     3841 ccctctcctc tctcgattcg atcctcgccg ggcgatcccc cgtccaggtg gtgcggacgt
     3901 gagcgagctt ccctcccacc gggagaggcg gcggtacctc ctcgcccggt tcgtgcccga
     3961 gggagcctcc cccgatcccc gcgaggtgta cgccgcggtg gtcgaggcct gcacctccct
     4021 ctggggggac gtggccgcct cggagatctc cccctcggtg atggcctggg aggggcatgc
     4081 catcctccgg tgccgccggg gaaccgagga ccggctctcg acggccctcg ccaccatcac
     4141 gaaggtcggg gacgtgcggg tcgcgatgag gagcctcgcg acgagcggaa ccctccacgc
     4201 catccggagc cgggcgaacc caaagcccgg gatcctctgg gagaaacagg tcgggatcgg
     4261 gggccggacc ttcctcgcgc gggcctatcc gggtcaaaag gttgatttga tcgaaaaagg
     4321 aattaaaggt caggaattgt tgtttttcgc tgacgaagac ggagaggtga ggtgatgcaa
     4381 ccccagtacc agatgggata tgaccgggcg atcacggtct tctcccccga cggaaggctc
     4441 taccaggtgg agtacgcccg cgaggcggtg aagaggggga cgacagcggt gggtatcaag
     4501 tgcaccgacg gcgtggtcct catcgtggac aagagggtgg cgacacggct cctcgagccc
     4561 tcctccatcg agaagatctt caagatcgac gagcacatcg gggttgcgag tagcggcctc
     4621 gtcggggacg cccgtgccct cgtggaccgg gcccgggtcg agtgccagat caaccgggtc
     4681 tcctacaacg aggccatcga cgtcgagacc ctcgcaaaga agctctgcga ccacatgcag
     4741 acctacacca tgttcggggg cgcccggccc tacgggacgg ccctcctcat cgccggggtc
     4801 tcggacggca ccacccggct cttcgagacc gacccgagcg ggaccctcct cgagtacaag
     4861 gcgacgggga tcgggaccgg ccggcccgcg gtcatgaagg ttttcgagga ggagtacaac
     4921 ccggaggccg gctgcaagga ggccatcctc ctcgggttaa aggccctcca tgcggcgacc
     4981 gaggggaagt tcgacgtgaa caccgtggag atcggcatcg tggaggagaa ggccggggca
     5041 ttccggaaga tgacccggga cgaggtcgcc tccttcgtcg aacagtttga gaagaccggc
     5101 gcggcatgat ccccctggac cgggcggtgg tcgcccggct cgagacccac ggggagcggt
     5161 tcgagatcct cgtggacccc gacctcgcgg tgaagatacg gcacggcgag cccgtggaca
     5221 tcgaggacgc cgtcgccgcc atcttcatct tcgagaacgc ctcccgggcc gagaagtcct
     5281 cggacgagtc cctccagaag gtcttccaga ccaccgattt ttccaccatc gcccggcgga
     5341 tcatcgagaa gggcgagatc cacctcaccg ccgagcagcg gaaggagctc ctggcagaga
     5401 agagggggca ggtcatcacc ttcatcgccc ggcatgccat caacccccag accggcctcc
     5461 cccatccccc ggcccggatc gagatggcca tggaggaggc gaaggtgtcc atcgacccct
     5521 tccggcccct cgacgatctc gtggccgata ccatgaaggc gctgcgcccc ctcatcccca
     5581 tcaggttcga ggaggtccgg gtggcggtca agatcccggc ggagcacgcg gcccgggcct
     5641 atggggacat cgattccttt gcccggatcg agcaggagga gtggcagaag gatggctcct
     5701 ggatctgcgt ggtgaagatc ccggggggca tgcagcagga gctcgagaac ctggtgagga
     5761 agatcacgaa gggcacgggg cagacccggg tgatctgacc ggcgccccga ttaagtatat
     5821 gaagggagag gtcaaaatta gagggacata tccggggagg atcgagaatg agcaccacca
     5881 agcagggatc caagggaagg gtatcgagga gtgccggccg gttcggccct cggtacggca
     5941 ggttcatccg gaagcgggtc gtggaggtcg aggatatctc ccgcgccacc cacacctgcc
     6001 cgagctgcga ccaggtcgcg gtgaggcgga aggggacggg catctggcag tgccggaagt
     6061 gtgggtacaa gttcgccggc ggtgcgtacg tcccccagac ccccaccttc cggatcgccc
     6121 agcgatccat cgagaagccg caggtcccgg aggagtaggc cccgaatgcc cggagtatac
     6181 aagtgcgccc ggtgcaagca caaggtcgag attgacgtga acgtccggtg cccgtactgc
     6241 ggtcaccgga tccttttcaa ggaacgcggg gccgggatca aggatctcaa ggcccgatga
     6301 tcacggtcac cacctcccgg agggccgacc ccctcgtacg ccggatcggg agggatttcg
     6361 ccttcgccac cgggggtacg tacgtcaccc gggggaaagg cggattgtcc cggcctccct
     6421 tctccggagg gatcgtgctc gtcctcagcc gggacgggaa agggatccgg acgcagatcc
     6481 tcagcgggtc agaggatgcc gccatcctcc ggttcccgga ggtcagggag gaatcccggg
     6541 caggccccct gagaaagggg ctctttaccg gcgatcgcga acttttttcc accctctctc
     6601 cccttctccc cgtgagcctg gaaggggatc tccctgatac cgttgtcctc gatggcatcc
     6661 aggggaggcg tatctccctc ctcgtgccgg gtgaggcatg aggcacacgg cctccttttc
     6721 cttcaggtct gaccgggccg gggagatctt ccaggccctc gcccccgaga ggggggagat
     6781 caacccgagg acgaaggcgc gggtgaccct cgagcagggg gacatccttg tcgtttccat
     6841 cgcggccggg gacatcccgg ccctccgggc ggccctcaac atgtggctcc gcctcgtctc
     6901 cgtcgccggg gagatgctcg agatggcaca ggcaaagggg aaggagcagg aaggcttcgg
     6961 ttgatccggg gggacggatg ccatcgggac ggggacagcg gggtcggagg cggaccggtc
     7021 cggggcgctt tgccgatgcg gtcatggtcg tgcacggtcc ggaggccttt gaccgggggg
     7081 acgtcgccct cctccaggac ctcctcggcc ccggggagac ggtcgtcgcg ggggtgatgg
     7141 cccggaccgc cgcagaggaa tcgggccttc cggtcatcat cgacggcggg atcccgagct
     7201 cggtcatcgg gaggatcccg ggtcccgaag ggaaccggga tcgtccgggg gggaaaaggg
     7261 cccctctgga gaggatcccg ggtcctgaag ggagacccgg cgccgaagga gccggggctg
     7321 tctcggggag gaggaggcgc gaagcggctc ctcctgtcca ggggggaagg ggcccctctg
     7381 gaccggggaa aggggccccc ctggacagga ccgaaagaag ggtgttcctc gtgaaccggg
     7441 gcaagacccc ggagtccggg aggatcttcg gggagatcgt cgcctcccgg ctcccccggg
     7501 gccgccccct cgtccagctg gagtgctcct cccggaccgt ctttctctgg aacgggagcg
     7561 gaaggagccg ggagttcgcc cggctcctcg ccggggagac cgggttctcc ttcacggaaa
     7621 caacggcggg ccgggaggag gagagtgcgg aacgggagat ccggggctgc atccccggcg
     7681 aggcggttct cgtcgagggg atcgtcatcg ggaccgccac cgcggggacg gtcgttctcc
     7741 gaaagagcga gggaaggata cgggtggtct cgggcctccg gcccaaggcc catggcctgg
     7801 aaaagctctc ccggctcggg gatatcgatc tcggccgggc atggtgcaag agcggacgca
     7861 tccggggggt ccccccccgg gcgggcgggc cggcgcctgc ggccgggagg gtcgtcgtca
     7921 tcgaccaccg cgggcatgac ctgtaccggt tcctcacccc cgatacctgc ggggtccttg
     7981 ccatcgggga tgacacgacc tccgtctgcg gccatatctg ctctcacctg ggtatccccg
     8041 tgttcggggt ggtggatggg gaccgggacg accttctcgt ctccagtttc ccgccgggct
     8101 cggtgatagc agaggtctcc gggggaagcg atgacgaggc cgggcgggag ctcgccgggc
     8161 acaaccagga cgagattatc gcctgggagg actgggtggc ccgggccctt gccatcctgg
     8221 ggaaccggtg ccgggtgatc caccggtccc cgccccggcg gaggaaccgc agaaaaagaa
     8281 aggctgatcc aggcccggaa acgccgacgg ccccctctcc ggggaggatg aaccttatta
     8341 acctttgcag gaaatgtcat agtatggagc ggtcccgttt cctgcccctc ctcctccccc
     8401 tcctcgccgc cctcggggct gcccaggaga cctccgccct tccggaggag ggcatccgcc
     8461 tcctggactt cgccttcctg tactacctcc ccgtcctcgc cggacttctc ctcgtgttcc
     8521 tcgtcttcac gggcgcgaac cgggtcctgc ggaggaggta cagcgtcgtg gaattcgtgg
     8581 gaacggccgc cggtttcctc ctctccctcc ttttcatcgc gaccgggttc ctgttcctcg
     8641 cctcggcggt cgttctcgca tgggcgacgg ccatcgggga catcacctac gccgccgcgg
     8701 aggagtgggc agttcctatc tcgaacgagg tccagtacct cctccagccg gccctcgacc
     8761 tgctctcggg cacggccgac tataccccct atctccagga catcctcctc ggcgtcctca
     8821 tcgccggggg cgcggctctc atcatcatcg gtgtgatcct gatctggaag ctccccaaga
     8881 cctccttcta cgagatcatc aagaccagcg aggataccac cttcaaggag gtgatgacca
     8941 ggggacggga gcccctcacc cccaccgtca ccttcagggt catcgaccgg ctcacgaacc
     9001 agccttcccc ggacgtgaag gtcatcctga agcacaagga gggggcaaag gtttacaacc
     9061 ggtacaccga cttcaacggc gaggtcatct tccagaagat cgacgggctt tactcccagt
     9121 actatgccta tgtcgagggt gacgaggaga ggaagcggta ctgggtcatc cggacggcga
     9181 ttggcgccga aaccgtgacc tgatggattc gagggtctcg atctcgtcca cacccttgtc
     9241 gtcccggatc cggatgaagc gggggaaccg gagggcaaac ccgctcgcat agttcgggga
     9301 ggtctggatc tcggagtatc cgacctcgaa aaccacctgg ggctcgaagg tgatctcctt
     9361 ccccttctcg gacacgacga gatccttcat aacctcgtag atggtcgcga gcatctcgtc
     9421 ggtgattccc gtcgccacct tgcccacggg gaggagcctc cccccgtcct ggcaggcgag
     9481 gaggaaggag ccgaagaagt gggccctcct tccctcgccc cactcgcccc cgataaccgc
     9541 gaggtccagg gtgtccacct ccggcttcac cttgatccag tgcttgcccc tgacgccggg
     9601 ctggtaccgg gagtccaggt ccttcaccat gatcccctcc tgccccgcgt cgagggcctc
     9661 gttgtagaac ttttcaaggg ccgccggttc gtcgctcacc agctgggggg cgatgtggtc
     9721 cttgagggcc tcctccaggt gtttcctccg ctcccggaag gggaggtcga tgagggtctc
     9781 cccgtcgagt acaaggatat cgaagatgta gggtacgagc tcgatctttc ccgcctgttc
     9841 ctcgatgtcg tacttccggc ggaaccgtcg gagcacaaac tggaagggca tgggcctccc
     9901 gcccttcatc gcgaccgcct ccccgtccag gatcacatct ctcttcgtgg cggcgaggag
     9961 ccgggtgacc acgtcgggga tggccttcgt cacctcttcg agcttccggg agtagacccg
    10021 gacctcgttc cccttcttgt ggaactggat ccgggtcccg tcatacttgt actcggcggc
    10081 gaccttcccg tgttccgaga ccatatccga gatggagccc tgctgggcga gcatcatctt
    10141 caccggccgg aagatctcga tgcggacggc ggagagggcc tccctcccct gccgggcgag
    10201 gcgggccacc tccccgaggt cgttcatcgc ctggtaggca tggtcgacct cccccggctc
    10261 caaggaaaag gccttcgcga tggcgtcccg gacgttcccc tccccaatcc cgatccggag
    10321 ctcaccgagg atgagccggg ccaggtacct cccctccagg ggctggacgc tcgcgaagag
    10381 cttccgcacg gcgaggagct tctcccgctg ggacttcttc ccctctgaga gggcgatggc
    10441 ctcgcaggcg gccgagacat ccccgatggt catctcctcg gaaaagaagg aggtctgggt
    10501 ctttcccacg agaaggagct ccacggccat ccccacgtcc ccctcggcgg agaccttcgt
    10561 ctccacggcc tccctcttct tccccaccac gtaggccacc gcctcgtaga ggaggttcgg
    10621 gccgatcccg agcttgagag gggaccagtc ggggaagatc cggcccatga tgaaccggac
    10681 gaagacgggg agctcccggt cgtcgagccg gggcagatcc tgggcaagga ggtcgatcat
    10741 ctcgagccgg cccgagatcg cctcgagcct ctcgcagatc tccgcgaact cccggaagag
    10801 catcgagcta tccctttcct ccacccttta tctgtatccc aaatataaag accacccgaa
    10861 cagctgcaga tagttgaatg agaccattct gatctttttt attggggtgc tctcggggga
    10921 ctacgccacc ccgccacacg ggcatccccc ggtgtcagtg accttcatgg tgaggcggtg
    10981 tacggaatga acgggctatc ctcactcggg gcgccaaagc ggcgggggcg agcgaagcga
    11041 gtccccagga gcgtccgctg atttgcagat ccctcaggtg ttagtttgac gggattcgta
    11101 ttcggggggc ggaaggtcaa ccctatagcg tcagggtcgg ggtgaacacg tgctggcgtg
    11161 cgacgaggat ctccacatcc ccgaggtaga aggccagccc ctccctcgcc gaagcgaggg
    11221 ccttttccgg cgtgttgttg atctccgaaa gatgggcgag gatcaccgcc ccgacgtctc
    11281 ccgcgatctc acgtaggcat gtcgccgcgg ccacgttgga gaggtgaccc cggcgggacc
    11341 ggatcctccg cttcagggat tccgggtaag gacctgtgcg gagcatctcc gggcagtggt
    11401 tactctcgag gaggaccccg tcgcagcgcg agagcccgct cacgagggag gggccgagga
    11461 acccggtgtc cgtgcagaag gcaaaccggc atcccttctc accgacgtag tacccgcagg
    11521 gctcccgggc atcgtgggag gtggggaagg cttcaagggt aaacccaccg agctcgaagg
    11581 gctcggaagg gtgcacggtg tggagggatg ggcagtgccc ccgggaggcc tcccggggga
    11641 gttccagcag ggtcccttcc gttccacaga tcgggacctt cagtttccgg gcgagagccc
    11701 agacccccct gacatggtct atgtgctcgt gggtgaggag gatgcccccg atgggatccg
    11761 gatccttccc cgcggcggtg agtctcgcga gaacctcccg ggccgggata ccgcagtcca
    11821 caaggagggc accgtcctct ccggaggcga ggatgcaatt ccccttcgac ccgctcgcaa
    11881 ggacggtgat ctccatgcag gctctccctg gaaaaggcta ccatagatct ggtgttttgg
    11941 agggatatat gcgatagatc caccgggaaa actgtaggcc acgggtccct tcacgtctcg
    12001 ggcagcacgt ggatggcggt cgccttcacc gataccctga cctctcttcc ctcggagagg
    12061 ccgaggtcct ctgctgaact ggcggtgacg agggccatga tttcgatacc gcagtcgatg
    12121 acgacctgga cgacgggtct cagggctaca acccgcttta tcctccccgg gaagacattc
    12181 cgcgcgctgg acttcacgcc ctcggcgaga tcgatggtta catcctccga gcggaagaga
    12241 aggatcgccc tcgttcctgg ttgcagtgtg gcgactgccc ggaagacctt tccggagacg
    12301 tcaacgatga cctcaccctc cctgttctct gtgacgaggc cggagaggat gttcccgacc
    12361 tttacgaacc tggcgatatc cttcgaggcc ggcctctgga agatctcgag agaattccct
    12421 acctgggtga gggtcccccc catgagaact ccgatgctcc cggcaagtcg ctgtccctgg
    12481 agcatgtcat gggtgctcat gacgagggtc atccccttct cccggttcag gcgcaggatg
    12541 atctcctcaa ggttctcggc ggaaggggga tcgaggttcg cggtaggttc atcgaggaag
    12601 aggatctcgg gatcggtgac cagggcccgg gcgagggcaa ccctctggat ctctcccccg
    12661 gagagcgtac gtgcaggacg cggggaatag ccggggagat ccacatcgtg aagcacgccc
    12721 ctcacccggc tctcgatcct gtccctgctc tcgtgccggt acttcaggcc gatcgcgata
    12781 ttttcatgga cggtccggtt aaagatgacg ggcttctgaa ggagcatccc catccttctg
    12841 cggagctgga accgggaact cggcgggagg gcatcctctc ccaggatcga gagcctccct
    12901 ccggtcgggg gctcaaggag atcgaggagg cggaggaggg tgctctttcc tgccccgctc
    12961 gggccgatga gaccgaagat cctgcccccg ggaatcgaga gggtgatctc cctgaggatc
    13021 cttttttctc cgtatgattt ggagaggcct tgagcctcaa tcgcgtccat gcctcacccg
    13081 cgtatcatga tatccaggtg ttcttcagga cagaccccga ctggatgagg ttcagggcga
    13141 tgttcacgag caaggcgacc ccgaggagga caatgccgag ggcgatggca agggcgaaat
    13201 ccccgatgga ggtttcgagg gagatggccg tggtgaggac ccgggtgtac ccccggatat
    13261 tcccgccgat gatcatggcg gcccccacct cggagatggc ccggccgaag ccgaggatca
    13321 cggcggcgag gatgccgaac ctcgtttcaa ggatgagggt tccgatcgac tggatcttcg
    13381 tcgcaccaag ggacaggacg gtggagcgca tcgccggatc gatcccgctc agggtcgaga
    13441 aggtgagccc ggtgatgagg gggaggacca ggatcatctg ggcgatgacc atgccgtagg
    13501 gggtatagag aaggctcagg aagccgaggg gaccctggtg cgagatcagg agaaagagga
    13561 gaagcccggc gagcaccgtg ggcagggcgt agagcgtctg caggatattc acgagcactc
    13621 gtttcccgaa aaactcatgg taatacagga gggcacccgc agggatcgag atcgaggccg
    13681 aaaggatggt agcggcaagg gagatctcca gggagaggag cgtgatccgc atcacctcgg
    13741 gattcccggt cacgatgagc tggatagcct ggatgaaccc gtcagcaatc tcgcccgcca
    13801 tctacctgtc tcccgatcga tcaagcctct ccaccctata tctcggtttc gaaggagagg
    13861 ggaaatccct ctaccccgag atccgtccac attgtggtaa acaaatgata aatgttaaat
    13921 aacttcatag gcattaatct gcctcatgaa gtctacgaga aaacattgtc tgggatgcct
    13981 gctcggtatc gtgatcctga cggcgatcct cctatcaggg tgcacccagc cccagaccgt
    14041 gccggcgaac ccgacaccct cggcgaccac ggtggccgcg actccaaccc ccgtcgttca
    14101 ctcaccgaac accctcctca tcgcaaccac gacctctctc tatgacaccg gtctcctcaa
    14161 tgcggtccag gacctctatg agaaccagac cgggattcac ctcaagatca cctcccaggg
    14221 aaccggtatc gccatccagg tcgcgacccg gggagactgt gacctcctcc tcgtgcattc
    14281 gccctcccag gagaaggcct tcatcgacaa gggcctgggt gtcaaccagc ggtgctttgc
    14341 gtacaactac ttcgagatcg tcgggccggc ctctgacccg gcaggcatcg ccaacctcac
    14401 ccccgaggcg gccttcacga agatccggac gctcgggctg aacaacaccc ccggtgtctt
    14461 cttcgcctcc cggggcgacc agtccggtac gcactccgcc gagcagaaca tctggaaggg
    14521 tgcaggcttc aactacacga gggacgtggc gacatcggga tcctggtacc tctccctcgg
    14581 gaagggcatg ggggagaccc tgaccgtcgc cggccagaag ggggcgtaca ccctcaccga
    14641 tgaagggacc ttccttgcct tcaagagcca gctgaacctc gtccccctca ttacgaaggg
    14701 tacctcgctc ctgaaccgat acagcgccat cgctgtgaat cattcggtga actcgaacgt
    14761 gaacatcgtc caggccgacc ggttcatcaa ctggctcgtc tccgacgagg gcaggcagtt
    14821 cgtcgggaat tacggggtgg aaaagtacgg gaagagcctc ttcacgccgc tcacccccga
    14881 tggatgcacc aaggccccct tcaactgcac ctgttcgggg gatgtctccc cgatctaatc
    14941 ctgttttccc gcgataatgg tgaccacctt ggccgaagat tgttccggtt ctccctgacc
    15001 cgggtcccgg aaatcttaaa caggttcccg gtcgattctc cgggcatatg tggaaggagc
    15061 tcgaggtcgg ggcctgggtc gcctcccgga agcagagcat ggaggacgtg cgggcgcagg
    15121 tccaggagat catcgggagg gtgagggccg agggcgacac ggccctccgg gccctctccc
    15181 ggcacgtgaa gctcaccgag atcgcggtct cggacgagga gcgggaagcg gcgtacagcg
    15241 aggttgaccc cgcagttacc gaggccctcg tggaggccga ggcccggatc acccggttcc
    15301 acgagctcca gcgcccccgg gacctctggc tccaggaggt ggagcccggg gtcgtcctcg
    15361 ggatgaagac aacgcccctc gacaggatcg ggatctacgt ccccggcggg cgggccgcgt
    15421 atccctcctc ggccctcatg tgcgccgttc ccgcacgggt cgccggggtg cgggagatct
    15481 gcgcctgctc cccgcccccg gtgagcccca tgaccctcgt cgccctggac atcgcggggg
    15541 tgagggagat ctaccgttcc ggcggggcgc aggccgttgc ggccatggcc ctcgggacgg
    15601 agaccatccc ccaggtgcag aagatcgtcg ggccggggaa cgtctttgtc acgatggcca
    15661 aggtcctcct ccgggaggag gcggagatcg atttcccggc cggcccgagc gagatcgcca
    15721 tcatcgccga cgacagcgcc gatccccgga tcctcgcctg ggacatcctc gcccaggccg
    15781 agcacgatcc ccgggccgcc tgcgtgctca tcacccccga ccgggccctc gcggagaagg
    15841 tggggaagca ggtggcgggg ctcgcgggga aggaaccccg caaggagatc ctcgcggagt
    15901 ccctcgagaa ctgcgggtac gtcctcgtga aggacctgga cgaggcggtg gcggcctcgg
    15961 acgggatcgc ccccgagcac ctctccatcc aggtgaagga tcccctcccg gtcctctccc
    16021 gggtacggca cgcgggggcc atcttcgtcg gcaggtactc ggcggtggcc tgcggggact
    16081 acgcggtcgg gacaaaccat gtcctcccca ccgcggggta cgcgaggctc tattcgggcc
    16141 tcgacgtgaa ccacttctgc aagacctcct cggtggagat cctctcccgg gaagggctcg
    16201 aggccatcgg cgacgcggtc gagaccatcg ccacggcaga gggcctccac gcccacgcgg
    16261 cctcgatccg ggcaaggcgt gagtaggtct ccccgtatgg gcgggcgggt tcccttccgg
    16321 ctggatgcac ccgggagcct cgaggaggca agggccatcc aggaggccgt cgcccgggag
    16381 gtggaggctg cctcccccgt ctccatcgac ggcgtgatgc gcatcgccgg cgcggatgcc
    16441 tcgtattctc ccgacgggag gaccgtacac gcggcggtgg ccgtcctcgg gctccccgat
    16501 ctcaacctgc tggagcggtg ctgggtctcc cgggaggtgc ccttccccta cgttcccggt
    16561 tactttgcct tccgggaggg accggccgtc atggcggcgg tctccggcct cacccggagg
    16621 cccgatctcc tcctcgtgga cgggcacggg cgcgcccacc cgaggcgcgc ggggatagcc
    16681 tgccacgtgg gcctcctcct cggcatcccg acggtcgggg tggcaaagcg gatcctctcg
    16741 ggagacgcgg gggagatcgg ccccgcccgg gggtcgaggt atgccatcat ggacaggggg
    16801 gagatcacgg ggatggcggt caggacgaag gcagggtccc gccccgtctt tgtctcggcc
    16861 ggctttgcca cggatctcga gacctcggtg gccgcggtca tttccacgac ggccagctcg
    16921 cgggtcccgg caccggtccg cgaggcccac cggatctcac gggaggtccg gtggagctcc
    16981 ctgcccccct ccttccgacc ctgacacggc ggtttgtttc aggaagagga tctgatccgt
    17041 ttcattgctc catacgggaa tccgtacggg gccggagggc agtcctgagc cggatcaggg
    17101 gagagaccat gtcggcctcc gggaagggca cgaaattccg ctccgggagg acgagctcgg
    17161 gaggggcttc cttcatccgc ccgccgttcc gggcggggag gagggaggcg acggcgagga
    17221 ggcccgagag gatggcgatg aggaaaacaa agccggcctc ggagaggagc ctcgccgcga
    17281 aaaggaacca gaggcctgcc agctggagga gaaacagtgc ggcgagggca accctcacgg
    17341 ggagaccgtg ggggaatctc ccccgtgagg aaagataccg cttgagacgc ctcccgttct
    17401 cctgggccgg gacccggtac ctatcacccc gcatagatga ggcgaagagc tccagggcct
    17461 ccttgaggtt ccccagccgg gccttcgccg cccccaggta aaacaggatc cccggctcgt
    17521 cgggccggag ctgacctgcc ttggcgagca actgaaccgc ctcttcggca aggacaggat
    17581 ccccgttcgg cccctgccgg cttacaagca gtgcggccag ctcgaggagg agaacccatc
    17641 tctccggccc ggaagtgagc ccgagggctc ttcggatcac ctcctcggac ccggccggat
    17701 ccccgctcct tgcaagggcg atggcgaggc cccgcgcgat ggcgctgtcc cggggatcga
    17761 ggagggccgc ccgccggaga tgggagaggg cttccccggc cctccccgcc tggagagcga
    17821 tggcccccag gcccgcccac gcccgggcgt caagaggatc gaggccggtg gcgcgcgtga
    17881 ggaggggctc cgcctgcccc caccgacccc gggcgcaggc aaggaggccc aggttcctgt
    17941 acccggctgg atcggactgc cgcacagccc cggcgaagtg agcctgtgcc tcgtccggtt
    18001 ttccctgccg ggaggcgatg atcccgaggc aggacagggc gggaccgcat gccgggaaaa
    18061 gggagagggc cctctttacg gcgatggccg catccgccag gtcacgggcc ttccccgacc
    18121 gggcaaggac catgcccgtg ttcacgagga tgagggggtc cctcggccgg gcacccgcaa
    18181 gcctttccat gatgccccgt gcgtccccca ttcttcctgc ggcaagcata agggcggcga
    18241 ggccatggaa gccctggaga tcgtcttcac ggtctgcaag ctcgaggaat atctccctcg
    18301 cgcccttcag gtcgcggatg gagaggagaa accacccgag atcggcacgg aggtgagggg
    18361 cacccccttg ggattccacg aaccgccgga gcattttccc tgcctccgtt cccctcccga
    18421 gtctttccat ggcgagagcc ttcgaaagga ccggctcggg ccaggccgga tcggcgatgg
    18481 cggccttctc gaaggccccg gatgcgagat cgggccggtc gaggtccatg cagagccacc
    18541 cctcctggta gaggatagag gcacccgggg ggtcgaggga aagaccctcc tgcaggtacc
    18601 ggcctgcctc gcccagccgg cccaggagcc ggagggcggc gatctgcccg gcccgcgctt
    18661 ccctgtttga ggggtcgagg gcgaggatcc gaccgaatgt gggaagggag agccccggat
    18721 cggctccccc gagctcatcg agggcctggt gatggaggga ggcgatcctc caggagagag
    18781 actgcctgcc ctcaccggtg atgttcatca cccgggtgaa ggtcctcgcc gccttcccga
    18841 accgtccctg gagatggtag aggaccccga actccttgag aaggtccacg ctctcggggt
    18901 aaatctcgag gccccgggtg aggaggtgct cggcctccct gagccttccc tcctcgagga
    18961 gggaggatac tctggcatac agggccggtt cactgcacgc cgcatccggt gtaccggtct
    19021 ccggagcccc gaatgccgaa ccgggagcct ccctgtgctg actgccctcc ggtcgggaac
    19081 gggttttccc cggggtgcct gccagggagg aggtatcgtc gtctcccgtg ggaagatcgg
    19141 gatccttcgg ggtcatgatg cgcaggggaa tggtggggat tccaggggat actacctgta
    19201 ccatgcagcc atggggtgaa gaagatttca ctctgtttcc ggactccctc ttccctgcct
    19261 cccaacccag caatgattta tccttcggga ggaagatcgt ccgggagatg aagagcgagt
    19321 tcagggggaa gacctactac ttctgcgccc ccggctgcaa gaagaagttc gacgcggacc
    19381 cggcgaagta cgcgacgtag ggaccagaga acggtctgga aagaagcggc cggatccccg
    19441 gcctgtccgc aatccttttc ccgtggcgtt ccagagaaag aggggagaat atgagttatt
    19501 ttgaggaaat aaggaagcgg ggccgggtgg taaacccctt cttcgtgatg atggggatcg
    19561 acgtggactc cttcggggaa gggaccgccc ggctctccat gagggtgcgc ccggacatgc
    19621 acaacggcgt gggatggatg cagggaggga tcttcaccgc catggccgac gaggccatgg
    19681 ccctcgccct ctactcggtg gttttacccg gcgaggggat cgcgaccatc tccgagacca
    19741 cctccttcct caagggagcc cggaaagacg atctcctcgt cgcgacaggc cgggtggtca
    19801 ggaagggacg cagggtcgcc ttcaccgaaa gcgaggtgag aagggattct gacgccgggg
    19861 aggtcctcgc acggtgcgcc gcggcgtttg ccatcgtgga aaaaccggac ccggcatcct
    19921 caggacacgg gtcccgtaaa aaaaggtgag ggatggggct ctgccctgcg tttcttaccc
    19981 tatcttgatc tgtggccgct gtgtggtcac gggtaacgga gtgtatccag cgccggtggg
    20041 ttccttcccc cgtccgccgg actcattctc ctgctggccg gactcgtttt cacccatgac
    20101 cttctgcttc ttcccgtcca cggtgatgaa attgtgccgc accacggtgt cggacccggc
    20161 gtcgtttgtc accgtgaggg tcacggtgta cttcccgggc ttctcataga catggacggg
    20221 gttgatgtcc gtggaactgg ctccgtcacc gaagtcccag acccgcgagg acgggtatcc
    20281 ccgggagagg tccaggaagg tgacggccag gggggctgct ccatcctggg ggtaggcggt
    20341 gaactgcgcc ttgggcgggt ttcccaccct cacgatgatg aagttctccc tgaccagggt
    20401 gtccgacccg ccgctgttcg tgacggtgag cttcacggtg tatttaccgg gcgccacgta
    20461 ggtgtgcagc gggaacatat ccgtggaggt ggtcccgtca ccgaaatccc acaggcgtga
    20521 gctcggggag ccctgggaca catccaggaa gaggacggtc atgggtgcgg tcccctgcct
    20581 cgggtaggcg atgaactgtg ccttcggcgg gtttgcaccg ttcacggtga tgaagtcctc
    20641 ccgggtcatg gtgtcagacc cgcccttgtt cttgacggtg agccttacgg tgtacttccc
    20701 ggccgcgtta aaggtgtgca gcgggaacat ctccgtcgaa gtgctcccat ccccgaagtc
    20761 ccacagccgg gtgctgggcg acccctggga gacgtcgatg aagagcaccg tcaggggtgc
    20821 cgtgccctgg cgcgggaagg cgaggaactg ggcctttggt ccgctgggaa tcgccacgag
    20881 ctggaagttg acgtcaatcg tctcaccggc gacgacggtc accagctgct ctgcgtcctc
    20941 gtaaccggcc atcgtgactt tggcgacatg gaatccaggt gcaaggtcgc ttatcgtggc
    21001 ctcggtgacc attccggtgt ccccatcatc gatgaagatc ttagctccgg agggagtcga
    21061 agtgacatga attgatcccg ttgtcgggat gggtaccagg gtgaaatctg cgtccacagt
    21121 ctccccggcg acgacggtga ccgtctgttc ggcggccaac tcgtacccgg caagggtgac
    21181 gttgacgaca tgatcaccgg gtgcgattcc atcgatggtt gattcggtgg tcttcccggt
    21241 gtcgacattg tctacgaata tctcagctcc ggagggagtc gaggtgacat gaatcgatcc
    21301 ggtggttgga accggtgtca ggacgaagtc ggcctgagcg gtttcaccgg cgacgacggt
    21361 gaccgtctgt tcggcggcca tctcgtaccc ggcaagggtg acgttcacga catggtcacc
    21421 gggcgtgata ccgtcgatgg tgaactcggt tgtctggccg gagtccacgt tgtcgatgaa
    21481 gatctcagct ccggaggggt ttgaggtcac gtggatcgat cccgttgtcg ggaggggtac
    21541 cagctggaag tcggcctgag cggtttcacc ggcgacgacg gtcaccgtct gttcggcggc
    21601 cgactcgtac ccggcaaggg tgacgttgac gacatgatca ccgggcgcga ttccatcgat
    21661 ggtgaactcg gttgtctggc cggaatccac gttgtcgatg aagatctcag ctcctgacgg
    21721 agtcgaggtg acatggatcg atccggttgt tggaaccggt gtcaggacga agtcagcctg
    21781 ggcggtctca ccggcgacga cggtcaccgt ctgttcggcg gccatctcgt acccggcgag
    21841 ggtgacgttc acgacatggt caccgggtgt gatcccgtcg atggtgaact cggtcgtctg
    21901 gccggagtcc gcgttgtcga tccagatctc ggccccggag gggtttgagg tgacatggat
    21961 cgatccggtc gttggaaccg gtgtcaggac gaagtcagcc tgggcggtct caccggcgac
    22021 gacagtcaca gtctgttc
//
LOCUS       DATGDI010000236        16744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_113693, whole genome shotgun sequence.
ACCESSION   DATGDI010000236 DATGDI010000000
VERSION     DATGDI010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 16744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16744
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113693"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..5416
                     /locus_tag="VKO45_08905"
     CDS             <1..5416
                     /locus_tag="VKO45_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HMA06029.1"
                     /translation="QTNFPVLVSLPFDSDLAASAQANGNDIVFTDSGGSTVLPHEIEN
                     FTKSSGALTAWVKLPQISSAANTDIYMYFGNSSVTNQQNSTAVWDTNYKAVWHHDHDL
                     LDSTSIGNTGTNSLSTMAAAKISNGREYPDVGNTYVDVGSNASIANLFAGGGTVSAWI
                     YPRSWGWASFGRIADKTNSTNQGFGWSFMVDNDAGDTQRSLRFGRGRTTQRGNWVTPT
                     DSLALNQWQYVVATYNEDSTANDPLLYVNGVSQTVTEASAPTGSVEPDDASNMRIGNY
                     GGGLNRTFYGTIDEVRLSTNIRTPEWIATEFANQNRPDLFVSKGSGESPCGGGGSPPS
                     WYECGWLYRRNITINKTAVSGSLADFPVLVNVTLENPAHAQGDGDDFFFTNGTGTKIP
                     HEIESYSGGTLTAWVKVPSVSSSANTTIYLYYGNSSVGSQENYAAVWDSNYKGVWHMA
                     EDPSITTDGDCGGGSYHLCDSTSRSNDGIADGTMTSGDRVTGKVGKAWDFDGTDDRIR
                     IKSTTDFDPDIITVETWLKTSLNDERRIMVNYATAPHPWYFTMEDNSTLGGATVLYSL
                     STAAEVNRNTPTAYSNGAWHHAVAVSDASASNIKIYMDGNLVSVIPHSGEGWGNAMTV
                     PTIASGDGSMFLGQLDEIRVSTGARSADWIKTEYNNQNRPDLFSSPGNEETSPCGGGG
                     GSPLVINPCSWTGRKKLTVNGTMISGTQTSFPVLVNLSSDSDLAANAQASGNDIVFTD
                     SGGTTVLPHEIENFTKSSGALTAWVKLPTLSGGTNTDIYLYYGNSSVGSQQNATQVWS
                     NGYAGVWHLGELVTDNAVASGVHKDSTSYGNNGNQYNNSRVTGKIGDAQNFEGDVRDE
                     FIEIPNSASTENLQEGDYSLEAWFNSDVTPPGTLDSDWNAYYGIVIKPGWHIGLRFDR
                     WAKFDSDHYLTGGTEVWVGGVSGKSSSRWYHVVSVLNRTGGSHRIYVDGVEEDYATFT
                     AGTAANEFNQVPWRLGIASTNNRGGNWEWPANGKIDEVRLSGIARSAQWIATEYANQN
                     RPDLFISRGSGESPCGGGGGSSLVINPCDWASRKKLTINMSMVSGTQASFPVLVNLSS
                     DADLANGTTGAQSSGNDIVFTDSGGTAVIPHEIENFTKSSGALLSWVKVPSISSGANT
                     DIYMYFGNSTKPSQRNVTGVWDTNYLAVYHLNQDPSGTVIDSTGHANLSSEGTMGAAN
                     LVNAKIGKGIQPTDYSSTIDHLTSTNTVTVQSFTMEGWGDANNWYNWMSFVDVNTHAA
                     GAYYRDHTSNPDADLYVDMANGITDNRQLSGVMATGLHHTVSRYNSSSQVLDAFVDGS
                     TTGFSYSALVKPSLTGYVGVGAWSYYDTQLNWGDPFRGTLDEVRISNIARSDGWIQTG
                     YNNQYRPHLFVSAGTREAPCGGGGSCSGVTVDTTSSGWAQAASGLTISHTASGSNRLM
                     LVGVSIQDSDTVPTDTTDSVKVSSITYNGTVLTKVGSKYNADDAMVEIWKLIAPDTGT
                     HNVVITLNRTLASQGFVIAGVTTFAGVDQTTPLGTFASQAGDDDNQINVTVSSATGEI
                     AFGVIANEYNSIITDAGQTERWNIHKVGSLDTTNGAGSTKTGASSVLLKWTLSSTYNH
                     WATAGISVKPCTVPTYTVNLQVGSMLDDCFRYFTNKLDCSTYHDDYVDTTSKDTTATT
                     STGLRFTSVAIPKNAAITKAYLTLRAASTSGSPGGGVIQGEASDNGAAFTEGAWSEFD
                     GRTRTTNSVPWSPSAWSVGTDYTSPELKTVIQEIVNRAGWGSGNSMVLFIDLNITGDS
                     PAYSYHNDPAYAPKLHIEYQ"
     gene            5654..10468
                     /locus_tag="VKO45_08910"
     CDS             5654..10468
                     /locus_tag="VKO45_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HMA06030.1"
                     /translation="MNPSGQRRPVGEKPAISEIVGTIALIAVVVIGIVIINVVIISSP
                     SRARIPSVEATMANQSTLITIAHQGGDPIPWGQFKILVDGVDRTASFNNSGPYPWSIG
                     QTLSYNASTLPSSAVVIYKGTGGDEVVILETKFPWGMALSSQGGGLGSVTKLTLIKTV
                     VNDNGGTRQVSEFPLFIDGAQVISGVPNLVSANTEHTVSENSQSGYTSTGWSGNCSSD
                     GKITLATSEDKTCFITNDDTLLVSWFNCSWAYRKNITIDHTKVQGAHTDFPVLISLDA
                     GSSGLNGKAQASGNDILFTLDNGLTKIPHEIERYSAGTLVAWVRVPSVNSTVNTTIMM
                     YYGNPGATNQQNPTTVWTNGYKGVWHLNNSFADSTTSHYDGTNYGTTDIAGQIGYGRA
                     FDGGTQYITTPSTDLKTATDFTISTWFKSSNTAFAHHLIWEGLSSANGWGESWYEQEM
                     HISLGEFVNPNQVSNKISFFYGDNDIGRDTGDIGINQTFSDTSGWHLVEAVVTGAGGS
                     SPGATLYLDGSPIGSDTGTQARTGRASWDTSLQFGKPGASTRYFQGNLDEVHIATTAR
                     SSNWIATEYANQNSPSSFYFVGSEVGSPCVGGGAQARLTLVKTVVNNNGGTKQVSDFP
                     LFINSTPATSGVSYSVPANLELTASETSQNGYGSSGWSGNCSSAGKITLAPGEDKTCF
                     IINDDIPTASWYNCGWPYRKNITIDHTKVTGSHNNFPVLVKLDAGTSGLNGKAQANGN
                     DILFTKDDGTTKIPHEIEYYSSGTLVAWVNVSPVTSAADTTIMMYYGNSSVGSQQSVA
                     NVWDGNYKAVWHLKENPSGTAPQMKDSTGTANHGTSAGSMTSGDQVAGQIDGSLDFDG
                     SDDYVQGPDYDITGAITVSAWINWDAVTADDGIVSKRTSSEVAGDWALRMDPSGTGTL
                     EWMIWSGVDNSNNVFSNSAVGAGAWKYVVVTFDDPTNTAKIYINGGLDKTDSTTITNS
                     LANTPEQIAIGWAGQGSQYFDGRIDEVRLSNNVRSAPWITTEYANQNSPSTFLSVGGE
                     VASPCSGSGQFIPNPAWNNCYWPYRKAITIDHTKVSGTQSGFPVLINLSSDSDLALHA
                     MANGYDILFTDASSPPNPLPYERELYKSSYGGLVAWVKVPQIQSGSDTTLYMYYGNPS
                     STDQQNKNAVWDSNFAAAWHLNEAVTAETTGGNHYDSTTNANTGVQYYNGAITGRMGG
                     AQYFNGGDGSVDWIDVSTPGGSSLLSPTSQLTMEAWVYLPTNPNPNYIEKILSLGDGW
                     SKGYTLGVQNSPYGIYPEIWNGSSGSCWLTSGSFNLNTWTHLATTWNQSSNLMIGYIN
                     GGQVGSTNCGPNPINTTGLPQLRIGAEGWSGAPYQWNLTGRVDEVRISKTARSPTWIA
                     TEYANQNTPSTFAYPGSQESYTCGGYGPYYVQSRGQSYSSVSQAQITLPASTTAGNLL
                     VFSFALTPQTVTSNGVTDTKSNTYNAAQMTTVGNYRVYTYNVPNAIGGSGPITATLAL
                     SGPATTLNISFLEYGGIATASPVDKTISGSGSGTAMNSGSQDTTQAVELLYGFGAGDY
                     SCQASSPLYNRDLANGRCAMDRTVSATGTYSVTATQSQSGNWALQMVTFKGA"
     gene            10653..11981
                     /locus_tag="VKO45_08915"
     CDS             10653..11981
                     /locus_tag="VKO45_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06031.1"
                     /translation="MPTSSRESGLSEIVGFILILAAIVMAVTLYATFGIPAQGREGEI
                     AHMNEVKDRFVEFKVNVDSLWSNRQCGTAIGTSFTLGTGGGSATGSFSIIPILSPVRT
                     TATLALNQRVEYINVTQDSLFMTDDPAQSWNESNFFSTTPGGITFSSAPRYFLINLTS
                     INIGQSHGVHLYPSSGSAWDAWVNVTPVFWNRSDVTVSTQTGGVSLMQGFIVFKNITS
                     SPSVYVVDLMNPAYGLNTALGAPQTITGVKNDDSITASYLTNYSYWPSVKSQNWNMGC
                     LEYRGQNEYWIPQTYYYQLGGVFLEQNDGNTVKVPPSISLSYSSGMPAVQINEILLDG
                     SGRIEGSGPVQVTSTLSSITNTQLASGNNTRFVNISILAQSNNASLMWQQAFQTAATK
                     AGFPSSIYTTNSSGLWSYLTVSASPNYGIRLSLSRTVLRTAIQTATPAGG"
     gene            11985..13448
                     /locus_tag="VKO45_08920"
     CDS             11985..13448
                     /locus_tag="VKO45_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HMA06032.1"
                     /translation="MRHDGVSEILGAIFLIAVAVLAMGIIVVILLSGPLPTRIPAFSG
                     LITNSSSTVFITHEGGDPLYPGTFQVLVDGKDETSNFTKSFTGPFTLGKVMNATLPSM
                     PRRVVLVFNTSWGGGTILASADLGETIPYTPPGWYSGAWQVRKKITINHNQVQGSLID
                     FPVLLSFADNGLKNNASSTGNDILFTASDGVTKLPHEIEGYTSNNGALVSWVKVPSLS
                     SSADTVIFMYYKNPGAPSQQNAVNVWTNGYAGVWHLGETSGTTRHDSTANNNDLVESG
                     GPIANSTSGKIGGAANFVRASTQYLWITDAAQTGLDITGPITLEAWALADENTTPPYQ
                     IFDKSRGTTCGSGDPPYFLRLNDPVAGAFCECFVVSGVCGTNPTDAQPGGVTISTGAW
                     YFLAGTYDGSFSRVYRNGVQTDSVSSSSGIFNSNGGFYIGSQVNAQYFDGLIDEARVS
                     NVNRSVQWLATEYNNQNSPASFSTIESTEQDRFTMRW"
     gene            13616..16207
                     /locus_tag="VKO45_08925"
     CDS             13616..16207
                     /locus_tag="VKO45_08925"
                     /inference="COORDINATES: protein motif:HMM:NF019407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HMA06033.1"
                     /translation="MDHSGVSEIIGTLVLVGIVVIGIVLAALLLFSHPAATKVPVFDC
                     IISNQSNTIYLYHRGGDSLSAGEYRILVDGVDRTANFTFMSPGTEPWSVGETLTGTMA
                     SLPRHVTIVFVSADGEATAISQADLVPVLEVPPHLPNYPMVAWSNSPAFGNITTLFQF
                     TDASSVDNLTSYFWNFNDANISSLRSTSHVFPCVQEYCTYEIDHGVTDSGNTSWAATA
                     WLNRSAWVTVYKNLTPTVTFTQDRTGGPLGMLSTISFTATPYGAIRVDSWYWTFGDSG
                     TSTSQNPSHAYTAEGTYTVTLTATNYTLGQNVTMKTNLIQVTAPWPSCSWPYRKKIIL
                     NHAKVPATLTNFTVLISLTDNDLKNRQPDGNDIVFTSSDGTTKLFHEVEAYTSSTGTL
                     VAWVKIPTLSSATDTILYMYYNNAGLSTQQDPPNAWDPNYMTVWHLNAGTSGSGAVKD
                     STGYLSGTGNGGLTLVNGMIDKGFQFDGTSGYVRIDNSATGSRLDFTGGPFTISTWFK
                     MVDVATSRHIFGKRDNNMKQYQMGVGTLPDVFISNNTNQSWGSTLSTSYPWYYYAWTV
                     DSAGWPSLYLNGSLDPTWGGDVRQPWKFTHEACDATIGARWTTVPTTAAWYKGILDEI
                     RVSNVSRTENWLTTEYNTENSPSTFFSNTTEENSGCQGVGLPTFVQKATPNQFTNKNT
                     PSITLSNVVAGDLIVVSINWTNNARTISSVKDGNNNNYALANGPTTWAGSEAASTYYY
                     ANAPSGANTITVTFSGNTYGTIYAAEYSGVNTSGPIDKTASSTGTGTALNSGSGATAV
                     PKELVYGFCASRSTASPAFPYTARDVTGGNFIADNSTWSMGTYSVTGTSTAGTAWVCQ
                     MATFKGT"
     gene            16458..>16744
                     /locus_tag="VKO45_08930"
     CDS             16458..>16744
                     /locus_tag="VKO45_08930"
                     /inference="COORDINATES: protein motif:HMM:NF019407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HMA06034.1"
                     /translation="MDVRDRAVSEIVGTLVLVGIVVIGIVLAGLLLFSHPAASRVPVF
                     DCIISNQSNTIYLYHKGGDSLSAGEYRILVDGVDRTANFTFMSPGTEPWSVG"
BASE COUNT         3679 a         5399 c         4630 g         3036 t
ORIGIN      
        1 ccagaccaac ttcccggtcc tcgtcagcct ccccttcgac tccgacctcg ccgccagcgc
       61 ccaggcgaac gggaacgaca tcgtcttcac cgactccggc ggctccaccg tcctccccca
      121 cgagatcgag aacttcacga aatccagcgg cgccctcacc gcctgggtga aactcccgca
      181 gatctcctca gcggcgaaca ccgacattta catgtacttc ggtaactcgt ccgtcacaaa
      241 ccagcagaac tcgacagccg tctgggacac gaactacaag gctgtctggc accatgacca
      301 tgacctcctg gactccacct cgatcgggaa cacagggacg aactctctgt ccaccatggc
      361 tgcggcaaag atcagcaacg gccgggagta ccccgacgtg ggcaacacct acgtggacgt
      421 gggatccaat gcctccatcg cgaatctatt cgccggtggc ggcaccgtct cggcctggat
      481 ctaccccagg agctgggggt gggcctcctt cggccggatc gccgataaga cgaattccac
      541 caaccagggt ttcgggtggt ccttcatggt cgacaatgat gccggagaca cgcaaaggag
      601 cctcagattc ggcaggggaa gaaccaccca gaggggcaac tgggtgaccc ccaccgattc
      661 cctcgccctc aaccagtggc agtacgtggt ggccacctac aatgaagact ccacagcgaa
      721 cgatcccctc ctctatgtga acggcgtttc ccagacggtg accgaggcct ctgcacccac
      781 gggcagtgtc gagcccgatg atgcctcgaa catgcgcatc gggaactacg gagggggcct
      841 gaaccggacc ttctacggta ccatcgatga ggtccgcctc tccacgaata tcaggactcc
      901 ggagtggatc gccaccgagt ttgcgaacca gaaccgcccc gacctcttcg tttcgaaggg
      961 atccggggag tccccctgcg gcggcggtgg ttcgccaccc tcctggtatg aatgcggctg
     1021 gctctaccgg aggaacatca ccatcaataa gactgcggtc tcgggaagcc tcgcggactt
     1081 cccggtcctc gtcaatgtta ccctggaaaa tcccgcacat gcccaggggg atggcgatga
     1141 cttcttcttc acgaacggga ccgggacgaa gatcccccat gagatcgagt cctactcagg
     1201 cggcaccctc accgcctggg tcaaggtccc ctctgtctcc tcatccgcga acacaaccat
     1261 ctacctgtac tacgggaact ccagcgtcgg gagccaggag aattacgcgg cggtctggga
     1321 ctcgaactac aagggggtat ggcacatggc cgaggatccc tccatcacga cggatgggga
     1381 ctgcggaggc ggcagttacc acctgtgcga ctccacttca cggtccaacg acggcatcgc
     1441 tgacgggacc atgacctcgg gcgaccgggt cacgggcaag gtggggaaag cctgggactt
     1501 cgacggaacc gacgacagga tccggatcaa atcgaccaca gacttcgacc cggatattat
     1561 caccgtcgag acctggctca agacgtcact gaacgatgag aggcggatca tggtgaatta
     1621 tgcgaccgct ccccacccgt ggtacttcac catggaggat aactccaccc tagggggcgc
     1681 taccgtcctc tactcgctga gtactgccgc cgaagtgaac cggaataccc cgaccgcata
     1741 cagcaacggg gcctggcacc atgccgtggc tgtttcagat gccagtgcaa gcaatatcaa
     1801 gatctacatg gatgggaacc tggtgtccgt catcccccat tccggtgaag gctggggaaa
     1861 tgccatgacg gttcccacca ttgcctccgg ggacgggtcc atgttcctcg gccagcttga
     1921 tgaaatccgg gtgtctaccg gggcacgctc ggccgactgg atcaagaccg agtacaacaa
     1981 ccagaaccgc ccggacctct tctcatcccc cgggaacgag gaaacatccc cgtgcggtgg
     2041 cggcggtggt tcgcccctcg tcatcaaccc gtgcagctgg accgggcgaa agaagctcac
     2101 cgtcaacggg acgatgatct ccgggaccca gaccagcttc ccggtccttg tcaacctctc
     2161 ctccgactcc gacctggccg caaatgccca ggcaagcggg aacgacatcg tcttcaccga
     2221 ctcgggaggg accaccgtgc tcccccacga gatcgagaac ttcacgaagt cgagcggcgc
     2281 cctcaccgcc tgggtgaagc tcccgaccct gtccggtgga acaaataccg acatttacct
     2341 gtactacggc aactccagcg tcgggagcca gcagaatgca actcaggtct ggagtaacgg
     2401 ctatgcaggg gtgtggcacc tcggtgagtt ggtgacagac aatgccgtcg cgagcggtgt
     2461 gcacaaggac tcgacctcct acgggaacaa cgggaaccag tacaacaaca gccgtgtgac
     2521 cgggaagatc ggggacgccc agaatttcga gggggatgtc agggacgagt tcatcgagat
     2581 ccccaactct gcctcgacgg agaacctcca ggaaggagac tactccctcg aggcctggtt
     2641 caactccgat gtcactcctc ccggcaccct ggacagtgac tggaacgcct actatggcat
     2701 tgtcatcaag cccggctggc acatcgggct caggttcgac cgctgggcga aattcgacag
     2761 cgaccactac ctcacgggag gtaccgaggt gtgggtcgga ggggtcagcg gaaagagcag
     2821 ctcccggtgg taccacgtgg tcagcgtgct caaccggacc ggcgggtccc acaggatcta
     2881 cgtggacggg gtcgaggagg actacgccac cttcaccgcg ggaaccgccg ccaatgaatt
     2941 caaccaggta ccgtggcgcc ttgggattgc gagtaccaac aaccgggggg gcaactggga
     3001 atggccggcg aatgggaaga ttgacgaggt gcgactgtcc ggcattgccc gttctgccca
     3061 gtggattgcc accgagtacg ccaaccagaa ccgccccgac ctctttattt cccgggggtc
     3121 cggggagtcc ccatgcggtg gcggaggcgg ttcatccctg gttatcaacc cgtgcgactg
     3181 ggcctcccgg aagaagctca ccatcaacat gtccatggtc tccgggaccc aggccagctt
     3241 cccggtcctc gtgaacctct cctcggatgc agatcttgcg aacggcacca ccggtgcgca
     3301 gagcagcggg aacgacatcg tcttcaccga ctcgggaggc accgctgtca tcccccatga
     3361 gattgagaac tttacgaaat ccagcggcgc cctcctctcc tgggtgaagg tcccctccat
     3421 ctccagcgga gcaaataccg acatatacat gtactttggt aattccacca aacccagcca
     3481 gcggaatgtg acaggcgtct gggatacgaa ctacctggcc gtgtaccacc tgaaccagga
     3541 cccgtcggga accgtcatcg attccaccgg ccacgcgaat ctcagctccg aaggcacgat
     3601 gggggcagcc aacctggtca atgccaagat cgggaaagga atccagccga cggattacag
     3661 ttccaccatc gatcacctga cctcgacgaa taccgttacc gtgcaatcct ttaccatgga
     3721 agggtggggg gatgccaaca actggtacaa ctggatgagt ttcgtcgatg tgaataccca
     3781 tgcggcgggc gcatactacc gggaccacac atcgaatcct gatgccgacc tgtacgtgga
     3841 catggcaaat ggcattaccg acaaccggca gctctccggt gtcatggcta ccggactgca
     3901 ccataccgtg agcaggtata acagcagctc ccaggtactg gacgcctttg tcgatggatc
     3961 caccaccgga ttttcgtatt ccgccctggt caagccctcc ctaaccggct acgtcggagt
     4021 tggtgcatgg tcatactacg acacccagct caactggggc gaccccttcc ggggaaccct
     4081 cgatgaggtg aggatctcca acatcgcccg gtccgacgga tggatccaga ccgggtacaa
     4141 caaccagtac cgcccccacc tcttcgtgtc cgcgggaact agggaggccc cgtgcggtgg
     4201 cggcggaagc tgcagcgggg tcaccgttga cacgacctcg tccggttggg cgcaggcggc
     4261 ctccggcctc accatctcgc acacggcatc cggttcaaac cgcctcatgc tcgttggcgt
     4321 ctcgatccag gacagcgaca cagtccccac cgataccacg gatagcgtga aggtgagttc
     4381 catcacttat aacgggacgg tactgacaaa ggtcggttcc aagtacaacg cggatgatgc
     4441 aatggtcgag atctggaagc tcatcgctcc agatacggga acccacaacg tggtcatcac
     4501 gttaaatcga acgctcgcaa gccagggatt cgtcattgcc ggcgtaacga ccttcgccgg
     4561 tgttgatcag accacgcccc tgggcacctt cgcctcgcag gcaggggacg acgacaacca
     4621 gatcaatgtc acggtctctt cggcaacggg cgagatcgcc ttcggggtca tagccaatga
     4681 gtacaactcg atcattaccg atgcaggcca gaccgaacgc tggaatatcc ataaagttgg
     4741 atcgctcgac acgaccaacg gtgccggaag caccaagacc ggtgcatcgt ccgtgctcct
     4801 caaatggacc ctgagcagca cgtataacca ctgggcaacg gccgggattt ccgtcaagcc
     4861 atgcacagtg ccaacatata cggtcaacct gcaggtgggc tccatgctcg atgactgctt
     4921 ccgctacttc accaataagc tggattgttc aacatatcac gatgactacg tcgataccac
     4981 gagtaaggat actacagcca ccacctcgac cggcttacgg ttcacgagcg tcgcgattcc
     5041 caagaatgcc gccatcacca aggcctacct cacgctccgg gcagcatcga cttcagggag
     5101 cccgggaggg ggggtgatac agggggaggc ttctgacaat ggcgcagcct tcacagaggg
     5161 tgcctggagt gaattcgatg gccggacgag aaccaccaac tctgtcccct ggtctccttc
     5221 ggcatggtcc gtgggcaccg attacacgag cccggagctc aagaccgtga tccaggagat
     5281 cgtgaaccgg gcgggctggg gaagcgggaa ttcgatggtt ttattcatcg acctgaatat
     5341 tactggcgat tcgccagcgt actcctacca taacgacccg gcgtatgcac cgaaactgca
     5401 catcgaatac cagtaaccaa tctctctttc cggagctgca aattctccta tttaaaggct
     5461 tccggaggag cagggagttc cgaaggggcc aaagagagag gatctgtctg gatcgcggag
     5521 ttccacccgc cccatgaaat tacacaagat atttatatac ttacccattc tataattatg
     5581 taaaggcagc cttgtactgg aaaacccttc tttttctggg tgatcaggcc ggggtgcccc
     5641 ggaagggtac cgcatgaatc catctgggca gaggagaccg gtaggagaga agcccgcgat
     5701 ttccgaaatc gtcggcacca tcgccctcat cgcggtggtg gtgatcggga tagtcatcat
     5761 caacgtggtc atcatcagct ccccctcccg tgcccggatc ccctccgtcg aggccaccat
     5821 ggccaaccag agcaccctca tcaccatcgc tcaccaggga ggggatccca tcccgtgggg
     5881 ccagttcaag atcctcgtgg acggcgttga ccggaccgcg agcttcaaca attccggtcc
     5941 ctatccctgg tccatcgggc agaccctgtc atataatgcc tccacgctcc cctcgagtgc
     6001 ggtcgtgatc tacaagggga ctggtgggga tgaggtcgtg atcctggaga ccaaattccc
     6061 ctgggggatg gccttatcgt cgcagggcgg tggactggga tccgtcacca agctcaccct
     6121 tatcaagacg gtggtgaacg acaacggcgg gaccaggcag gtcagtgaat tccccctctt
     6181 catcgatggc gcccaggtaa tctctggtgt ccccaacctg gtcagcgcca acaccgaaca
     6241 cacggtatcg gaaaactccc agtccggcta cacctccacc ggctggagcg gcaactgcag
     6301 cagcgacggg aagatcaccc tcgcgaccag tgaagacaag acctgcttca tcaccaacga
     6361 cgataccctg ctcgtttcct ggttcaactg cagctgggcg taccggaaga acatcaccat
     6421 cgaccacacg aaggtccagg gagcccatac cgatttcccg gtcctcatca gcctcgatgc
     6481 ggggagctcg ggcctcaatg gcaaggccca ggcgagcggc aatgacatcc tcttcaccct
     6541 ggataacggc ctcaccaaga tcccccacga gatcgaaagg tactccgccg gaaccctggt
     6601 cgcctgggtg agggtgccct ccgtgaactc cacggtgaac accaccatca tgatgtacta
     6661 cgggaacccc ggcgccacga accagcagaa cccgacaacc gtctggacga acgggtacaa
     6721 gggagtatgg cacctgaaca acagcttcgc tgacagcacc accagtcact acgacggcac
     6781 caattacgga accacagaca ttgcagggca gatcgggtat ggccgggcct tcgatggagg
     6841 cacccaatac atcaccaccc cgagtacgga cctgaaaact gcgacggact ttaccatcag
     6901 tacctggttc aagtcctcaa atactgcctt tgcccaccac ctgatctggg agggcctgag
     6961 ctcggccaac gggtggggtg agtcatggta cgagcaggag atgcacatct cgctggggga
     7021 gttcgtgaat ccgaaccagg tgtccaacaa gatttccttc ttctacgggg acaatgatat
     7081 cggcagggat acgggagata tcggtatcaa ccagaccttc tccgacacct ccggctggca
     7141 cctcgtggag gcagtggtca cgggggcggg cggttcgagc cccggcgcaa ccctgtacct
     7201 ggatgggagc cccatcggga gcgacacagg gacccaggcc aggacggggc gggcaagctg
     7261 ggatacatcc ctccagttcg gaaagccggg ggccagcaca cggtacttcc aggggaacct
     7321 cgatgaagtc cacatcgcca ccaccgcccg gtccagcaac tggatcgcca cggagtacgc
     7381 gaaccagaac agcccctcga gcttctactt tgtcgggagc gaggtcggat ccccgtgcgt
     7441 gggaggtggg gcccaggcca gactgaccct ggtgaagaca gtggtgaaca acaacggcgg
     7501 caccaagcag gtgagcgact tcccgctctt catcaacagc acccctgcca catcgggtgt
     7561 ttcctacagc gtgcccgcca acctcgagct caccgcatcc gagacctccc agaacgggta
     7621 cggttcgtca gggtggagcg gcaactgcag ttcggccggg aaaatcaccc tcgcgcccgg
     7681 cgaggataag acctgcttca tcatcaacga cgatatcccc acggcctcct ggtacaactg
     7741 cggctggccc taccggaaaa acatcaccat cgaccacacg aaggtcaccg ggagccataa
     7801 caacttcccg gtccttgtca agctcgatgc cggcacctca ggcctcaacg ggaaagccca
     7861 ggcgaacggg aacgacatcc ttttcacgaa ggacgacggg acgaccaaga tccctcacga
     7921 gatcgagtac tactcttccg ggaccctcgt ggcatgggtg aatgtctccc cggtgacttc
     7981 cgcggccgat accaccatca tgatgtacta cgggaactcc tcggtgggca gccagcagag
     8041 cgtggcgaac gtctgggacg gcaattacaa ggcggtatgg catctgaaag aaaatccgtc
     8101 ggggaccgcc ccccagatga aggacagcac gggaaccgcg aaccatggaa cctcggcagg
     8161 gagcatgacg agcggggacc aggttgcagg gcagatcgat ggcagcctgg acttcgacgg
     8221 atccgatgac tatgtgcagg ggccggatta cgacatcacc ggagcgatca ccgtctcggc
     8281 atggattaac tgggatgcgg tcaccgcaga cgacgggatc gtttcgaaac gaacgtccag
     8341 tgaggtcgcg ggggactggg cactgagaat ggatccatcc ggaaccggta cccttgagtg
     8401 gatgatctgg agcggtgtcg acaactccaa taacgtgttc tcgaactctg ctgtcggtgc
     8461 cggtgcatgg aagtacgtcg tcgtaacctt cgacgatccg acgaacacgg cgaagattta
     8521 catcaacggg ggcctcgaca aaaccgattc cacgaccatc acgaacagcc tcgccaacac
     8581 gccggaacag atagccatcg ggtgggcggg gcagggctcc cagtatttcg acgggcgtat
     8641 cgacgaagtg cggctctcca acaacgtccg gtctgccccg tggatcacca ccgagtacgc
     8701 gaaccagaac agcccctcga ccttcctctc ggtcgggggc gaggtcgcat ccccttgctc
     8761 ggggtccggc cagttcatcc ccaacccggc gtggaacaac tgctactggc cctaccggaa
     8821 ggccatcacc atcgaccaca cgaaggtctc cgggacccag tcaggcttcc cggttctcat
     8881 caacctctcg tctgacagcg acctcgcgct ccacgcgatg gcgaacgggt acgacatcct
     8941 ctttaccgat gcctccagtc cccccaaccc gctcccctat gaacgggagc tctataagtc
     9001 ctcatacggg ggcctcgtgg cctgggtgaa ggtcccccag atccagtccg gctccgatac
     9061 caccctctac atgtattacg gaaacccctc ttcgacggac cagcagaata agaatgcagt
     9121 ctgggactcc aacttcgcgg ccgcctggca cctgaacgag gcagtgaccg cggaaacgac
     9181 gggaggaaac cattatgact cgaccacgaa cgcgaacacc ggcgtccagt attacaatgg
     9241 tgccataaca ggaaggatgg gaggtgcgca gtacttcaac ggtggtgacg ggtcggtcga
     9301 ctggatcgac gtttcgacgc caggaggttc gagcctgctt tccccgacct ctcagcttac
     9361 catggaggca tgggtatacc tccccacgaa tcccaatccc aactacatcg aaaagatcct
     9421 gagcttgggg gatgggtggt cgaaggggta taccctcggc gtccagaaca gcccctacgg
     9481 catctatccc gagatctgga atgggtcctc aggatcctgc tggctcacat cggggagttt
     9541 taacctgaat acgtggaccc acctggcaac gacctggaac cagtccagta acctcatgat
     9601 cggctatatc aacggcgggc aggttggcag cacgaactgc ggcccgaacc ccattaacac
     9661 caccggactg cctcagctga ggataggggc ggaaggctgg agcggagccc cctaccagtg
     9721 gaacctcacc ggccgggttg acgaggtccg gatctcgaag accgcccgat ccccgacctg
     9781 gatcgccacc gagtatgcga accagaacac cccgtcgact tttgcctacc ccggaagcca
     9841 ggagtcctac acttgcgggg gttacgggcc ctactatgtc cagagcaggg gccagagcta
     9901 cagctccgtg agccaggccc agattaccct ccctgcatcc accaccgccg ggaatctcct
     9961 cgtcttcagc ttcgccctca cgccccagac ggtcacctcc aacggggtca ctgacacgaa
    10021 gagcaacacc tacaacgccg cccagatgac caccgtggga aactaccggg tatacacgta
    10081 taatgtcccc aatgccatcg ggggatccgg acccatcacc gcaaccctcg ctctctccgg
    10141 gcccgccaca accctgaata tatccttcct cgagtatggc gggatcgcca cggcaagccc
    10201 cgtggacaag actatatccg ggtctggctc cggtacagcc atgaactccg ggtcgcagga
    10261 caccacccag gccgtggaac tcctgtatgg tttcggggca ggggattact cctgccaagc
    10321 gagttccccc ctgtataacc gggatctcgc gaacgggcgt tgtgccatgg accggaccgt
    10381 ctcggccaca ggcacctaca gcgtcaccgc tacccagagt caatcgggca actgggccct
    10441 ccagatggtg accttcaagg gcgcctgaaa aggaaacggg atggaagacc atggcaattc
    10501 cctcctctta tgcgcgagat ccggagcggg gccaggggat cccggaggga aatccccttt
    10561 ccggctccgc gcccgcggtt tccggcccgt tctcctccgg gtcttcgccg acagaggttt
    10621 atatattatc ccgtgaatat tttcctgaga acatgcccac atcctcccgg gagagcgggc
    10681 tctccgaaat cgtcggattc atcctcattc tcgcggccat cgtcatggcc gtcaccctgt
    10741 acgccacctt cgggataccg gcgcagggaa gggaagggga gatcgcccac atgaacgagg
    10801 tgaaggaccg gttcgttgag ttcaaggtca atgtcgattc cctctggtcg aaccggcagt
    10861 gcgggaccgc cattggcacc tccttcaccc tggggaccgg cggcggcagc gccacgggtt
    10921 ccttcagcat catccccatc ctttcgccgg tcaggaccac cgcgaccctg gccctcaacc
    10981 agcgggtcga gtatatcaac gtcacgcagg actccctctt catgacggac gacccggccc
    11041 agagctggaa cgagtcgaac tttttctcca ccactccggg cggcatcacc ttcagctcgg
    11101 ccccccggta tttcctgatc aacctcacgt cgatcaacat cgggcagagc catggagtcc
    11161 acctctatcc cagctcggga tctgcatggg atgcctgggt gaatgtgacc ccggtgttct
    11221 ggaaccggtc cgatgtcacg gtgagcaccc agaccggggg agtctccctc atgcagggct
    11281 ttatcgtatt caagaacatc acctcttcgc cgtcggttta cgtcgtcgac ctcatgaacc
    11341 ccgcctacgg gcttaatacg gctctcggtg caccccagac catcacgggg gtgaagaatg
    11401 acgattccat cacggcctcc tacctgacca attacagcta ctggccgtcg gtgaaaagcc
    11461 agaactggaa catgggatgc ctcgagtacc ggggccagaa cgagtactgg attccgcaga
    11521 cctactacta ccagttgggg ggagtcttcc tcgagcagaa cgacgggaac accgtgaagg
    11581 tccccccctc gatttccctc tcttactcga gcgggatgcc ggcggtgcag attaacgaga
    11641 tcctcctcga tggatccgga cggatcgagg ggtccgggcc tgtgcaggtt acctccaccc
    11701 tttcctccat cacgaacact cagctcgcat ccgggaacaa cacccggttc gtgaacatca
    11761 gcatcctcgc ccagtcgaac aatgcctccc tcatgtggca gcaggccttc cagacggccg
    11821 ccaccaaggc gggattccct tcgagcatct ataccacgaa cagcagcggt ctctggagct
    11881 acctgaccgt ctcggccagc cccaactacg ggatccgcct ctccctgagc cggaccgtgc
    11941 tcagaaccgc catccagacc gcgacccccg cgggagggtg acccatgaga catgacggcg
    12001 tgtccgagat cctgggtgcc atttttctca tcgccgttgc cgtcctcgcc atgggcatca
    12061 tcgtcgtgat ccttctctcc ggacccctgc cgacccgcat cccggccttc tccggcctca
    12121 tcacgaactc cagcagtacc gtgttcatca cccacgaggg aggcgatccc ctttacccgg
    12181 gaacgttcca ggttctcgtg gacggcaagg atgagacctc caacttcacg aagtccttca
    12241 ccgggccctt caccctggga aaggtcatga atgccaccct gccctccatg ccgaggcggg
    12301 tggtgctggt cttcaatacc tcgtggggtg gagggaccat ccttgcctcg gccgacctgg
    12361 gggagaccat cccgtatact cctccgggct ggtattctgg ggcctggcag gttcgtaaga
    12421 agatcaccat caaccataac caggtccagg gaagtctcat cgatttcccg gtcctgctca
    12481 gtttcgcgga caacggcctc aagaataatg catcctcaac cgggaacgac atcctcttca
    12541 cggcttcaga cggggtcacg aagcttcccc atgagatcga gggctatacc tcgaacaacg
    12601 gggccctcgt ctcctgggta aaggtcccga gcctctcatc gtcggctgac acggtgatct
    12661 tcatgtacta caagaacccg ggggccccga gccagcagaa tgcggtgaac gtctggacga
    12721 acggctatgc aggagtctgg cacctggggg agaccagcgg aacgacgcgc catgactcga
    12781 cggccaacaa caatgacctt gttgagtccg gcgggcccat cgccaattcc acgagcggga
    12841 agataggggg cgccgcgaat ttcgtccggg cgagcaccca gtacctctgg atcaccgacg
    12901 ctgcccagac gggactcgat atcaccgggc ccatcaccct cgaggcctgg gcactggcgg
    12961 acgagaatac gactcccccg taccagatct tcgacaagtc gaggggaaca acgtgcggca
    13021 gcggggatcc cccatacttc ctgcgcctga acgaccccgt cgcgggggcc ttctgcgagt
    13081 gtttcgtggt gagcggtgtc tgcggcacca atccgacgga tgcgcagccg ggcggtgtca
    13141 ccatcagtac tggcgcctgg tacttcctcg cgggaaccta cgacggctcc ttttcccggg
    13201 tctacaggaa tggggtccag acggacagcg tttcgtccag ctcaggcatc ttcaacagca
    13261 acgggggctt ttacattggg agccaggtga atgcccaata cttcgatggg ctcatcgacg
    13321 aggcccgggt ctcgaacgtc aaccgcagcg tccagtggct cgctaccgag tacaacaacc
    13381 agaacagccc cgcctccttt tccaccatcg agagtacaga gcaggaccgg ttcacgatgc
    13441 gctggtgacg ccggctcagc gagtcgcccg gaggcggggt ctcggaaaaa tccctgaatt
    13501 aacaatctat tttataaatt gattcaaata accatattct ttcatagaaa tctatatata
    13561 gtaagagtgt tagatattcc cacgggagag ggacctctcg ccgggagatg gggccgtgga
    13621 tcattccggt gtttccgaga tcatcgggac cctggtcctt gtcgggatcg tggtcatcgg
    13681 tatcgtcctc gccgcacttc tcctgttctc ccacccggcc gccaccaagg tccctgtctt
    13741 cgactgcatc atctccaacc agtcaaacac catctacctg taccacaggg gcggggactc
    13801 cctcagcgca ggggagtaca ggatcctcgt ggacggggtg gaccggacag cgaactttac
    13861 cttcatgagc cccggcaccg agccctggtc cgtcggggag actctcacgg gtaccatggc
    13921 ctcccttccg aggcacgtga ccatcgtctt cgtctctgcg gacggggagg cgaccgccat
    13981 ctctcaggcg gacctggttc ccgtcctgga agttcccccc cacctgccca actacccgat
    14041 ggtggcctgg tccaattccc cggcctttgg gaatatcacc accctgttcc agttcaccga
    14101 cgcatcctcc gtcgataatc tcacctcgta cttctggaac ttcaacgatg ccaacatatc
    14161 atcgttgagg tccacctccc acgtcttccc ctgtgtgcag gagtactgca cctatgaaat
    14221 cgaccacggg gtcaccgatt ccggaaacac ctcctgggcg gccaccgcct ggctgaaccg
    14281 gtcggcgtgg gtcacggtgt acaagaacct gacccccacc gtcaccttca cccaggacag
    14341 gacagggggg cctctcggca tgctctccac cattagcttc accgccaccc cttacggggc
    14401 catcagggtc gattcctggt actggacctt cggggacagc ggaacctcga cctcccagaa
    14461 tccgtcccat gcgtacactg cggagggaac gtacacggtg accctcaccg ccaccaacta
    14521 caccctcggg cagaacgtga cgatgaagac aaaccttatc caggtcaccg cgccctggcc
    14581 cagctgcagc tggccctacc ggaagaagat tatccttaac cacgcgaagg tcccggctac
    14641 cctgacaaac ttcaccgttc tcatcagcct caccgataat gacctcaaga accggcagcc
    14701 cgacgggaac gacatcgtct tcacgtcatc ggacggaacg accaagctct tccacgaggt
    14761 cgaggcctac acgagctcga ccggaaccct cgttgcctgg gtgaaaatcc cgaccctctc
    14821 ctctgccacc gataccatcc tctacatgta ttacaacaac gcgggcctct ccacccagca
    14881 ggacccgccg aatgcctggg acccgaacta catgactgtc tggcacctga atgcaggtac
    14941 gagcggttcc ggggctgtga aggattccac gggatacctg agtggaaccg gcaacggagg
    15001 cctgaccctt gtcaatggaa tgatagacaa aggtttccag tttgacggta cgagtggcta
    15061 tgtaaggatt gacaattctg cgacaggcag ccggctcgat ttcacgggcg ggccctttac
    15121 catctcgaca tggttcaaga tggtggatgt ggcaacttcc cggcacatct tcgggaagcg
    15181 ggataataac atgaaacagt atcagatggg ggtcgggact ctcccggatg tgttcatcag
    15241 taacaatacc aaccagagct ggggttcgac cctgagcacc tcgtatccct ggtattacta
    15301 tgcatggacc gttgacagtg ccggctggcc atctctgtac ttgaatggtt ccctggaccc
    15361 tacctggggc ggggatgtcc gccagccgtg gaagttcacg cacgaggcat gcgatgccac
    15421 catcggggcg cgatggacca cggtcccaac gacagctgcg tggtacaagg gtatcctcga
    15481 tgaaatccgc gtctcgaacg tgtcccggac ggagaactgg ctcaccaccg agtacaacac
    15541 agagaacagc ccgtccacct tcttctccaa cacaacggag gagaacagcg gctgccaggg
    15601 tgtcggcctg cccacctttg tgcagaaagc gacccccaac cagttcacca ataaaaacac
    15661 gccctccatc accctgagta atgtcgtcgc gggcgatctc atcgtggtca gcatcaactg
    15721 gaccaacaat gcgagaacca tcagctcggt caaggatggc aacaacaaca actatgccct
    15781 tgccaatgga cccaccacct gggcaggatc cgaggcggcg tcaacatatt actatgccaa
    15841 tgctcccagc ggggccaaca ccattacggt tacctttagc gggaatacgt atggcaccat
    15901 ctacgcagcc gagtactccg gtgtaaacac ctcgggtcct atcgacaaga cagccagcag
    15961 taccggcacc ggaaccgcgc tcaacagcgg ttcgggtgcg accgctgtgc ccaaggaact
    16021 ggtctatgga ttctgtgcat cccgttccac ggcttctccc gccttcccct acacggcaag
    16081 ggacgtcaca ggtggcaact tcatcgcaga caactccacc tggtccatgg ggacgtatag
    16141 cgtcaccggc accagcactg caggcacggc ctgggtatgc cagatggcca ccttcaaggg
    16201 gacctgacgg ggtagtacat aaatgaattc gtcgctcggt ccccgaatcc cccctggccc
    16261 tcctcaaata gacccgttcg tatcaacgat aatcggaggg atcacccttg aatggcggat
    16321 gaaatcataa tgatatttat atagatatgc cttcaataat cagataagga caccccctgt
    16381 accagaaatc gccccgattt tgactccctc agctcaggaa ggcccccgga atgcatgtat
    16441 ccatccgaag aagggaaatg gacgtaaggg atcgtgcggt ctccgagatc gtcgggaccc
    16501 tggtcctcgt cgggatcgtg gtcatcggga tagtcctggc aggccttctc ctcttctccc
    16561 acccggccgc ttccagggtc cctgtcttcg actgcatcat ctccaaccag tccaatacca
    16621 tctacctgta ccacaagggc ggggactccc tcagcgcagg ggagtacagg atcctcgtgg
    16681 acggggtgga ccggacagcg aactttacct tcatgagccc cggcaccgag ccctggtccg
    16741 tcgg
//
LOCUS       DATGDI010000237         5748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_142506, whole genome shotgun sequence.
ACCESSION   DATGDI010000237 DATGDI010000000
VERSION     DATGDI010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142506"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(380..3166)
                     /gene="leuS"
                     /locus_tag="VKO45_08935"
     CDS             complement(380..3166)
                     /gene="leuS"
                     /locus_tag="VKO45_08935"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HMA06035.1"
                     /translation="MPKGIDLHELEERYRDRWAAEFEANPSGKEKYYLNVAFPYPSGA
                     MHVGHGRTYTVPDIVARFWRMRGRQVLFPMGFHVTGTPVIGISKRIARKDPKAVWLYR
                     DLYGVPQEVLDTFTDPMVIVRYFAGEYERIMRALGLSIDWRRRFITVDPQYSKFIEWQ
                     YGHLYEQGHVVKGAHPVKFCPQCDNPVGDHDLLEGDKAEIIRFVLVMFRYGDAFIPTA
                     TLRPETVYGVTNLWVNPEVTYVRAEVDGKAWIVSREAAEKLKMQDHTVVTGAEVRGRD
                     LVGKKASHPLSGEIPILPAPFVDPEMASGMVMSVPAHAPYDYIALRDLQEQGLYPEIR
                     PRSLLILEGAGEFPAKEAVEKAGIRDQKDPKLESVTQEIYAAEFARGKMRPEFGGKSV
                     RQARDEVADRMLAEFGSAVMYDFDTRPVVCRCGGRVMVRILHDQWFLAYSDPAWKAEV
                     HDQLGRMALVPPETRAEFERTVDWLKDWACTRKVGLGTKLPWDRNWIVEPLSDSTIYM
                     AYYTIAHHMKKLPPENLTPEVFDYLLLGKGTPSTLSPEILEPIRKELLYWYPYDFRFS
                     AKDLISNHLTFQLFHHRALLPPELQPKGMVVFGMGLLSGAKMSSSKGNVFLLEDAIEE
                     FGADTVRMFLTGSAEPWQDFDWRNELVLSVKKQIERFWGTIHELAAAKGTAGGPLDTW
                     LESRLQGRIARATKALELFQTRQALQECFYGIEADLKWYRTRMGEGSAPGPAARALAS
                     AWVRLLAPFIPYTCEALREALGEKGPVAFAPWPVADEGKIRIDAELGEDLLARTLEDI
                     GSITKILPVTPKEIQILVAPEWKRSIFTTIAGSPERREVVKEIMKDPGMRKRGKEAAE
                     AVKQITALVHKLSPVQVEGLLAHPVDEMTVFESAREFLGREFRVPVKILSAGDSDHPK
                     AKQALPYKPAIVIG"
     gene            complement(3178..5037)
                     /locus_tag="VKO45_08940"
     CDS             complement(3178..5037)
                     /locus_tag="VKO45_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PINc/VapC family ATPase"
                     /protein_id="HMA06036.1"
                     /translation="MEIVPDTSAVIDGRITQMIRTGEYQGAVIVIPEAVVAELEAQAN
                     QGREIGFSGLTELQELSRMAKEGTIELKYVGERPSLDQVKLASGGEIDALIRKVAIEH
                     EARFVTSDVVQAEVARAKGLDVIYLKPQVGDFAPLAIDQFFDENTMAVYLKERMRPVA
                     KKGSLKEVRLVTIRDAPLSEYELKAMAQEILERAKRDPDGFIELEKRGATVIQIGSLR
                     IAIARRPFSDGMEISAVRPVGKVTLENYEKAALIKKRIIGDRRGILIAGPPGSGKTTL
                     AQSIATFLSDHGLVVKTMEAPRDLQVPDNITQYTSLEGSMQSTGDVLLLVRPDFVIFD
                     ELRKNEDFTTFADMRLAGVGMIGVVHALEVQDALQRVLGRVDFGVLTQVVNTIFFVAD
                     GEVTKMYDLQFGMEVPSGMAGESRPRPVILLRNQETGTIEFEVFRHEGESIVMPARRE
                     EGARAPATPARGEERLSRNLLEKEIQREIGRYTAGYVDVHMISDNKAVVYIEDKDVPA
                     AIGKGGKNVSGIVNKLSVGIDIRPRSELERPVSEEEVQLEGGFRIRTDRKQLSIIATE
                     HRGKIVDLFAGKEYLFTATVGEDGEIRLARNSTIAQEMMRRLTNKETIRVKPV"
     gene            complement(5153..5524)
                     /gene="hisI"
                     /locus_tag="VKO45_08945"
     CDS             complement(5153..5524)
                     /gene="hisI"
                     /locus_tag="VKO45_08945"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HMA06037.1"
                     /translation="MDLKFTDGLIPVVVVDTRTREVLMMAYANREALDLTRKTGLAHY
                     FSRSRGRIWKKGEESGHLQRIVRILVDCDEDTLLYEVDQMGVACHTGHRSCFYRTLAG
                     EEITPRMFDPDEVYRKKEKKD"
     gene            complement(5542..>5748)
                     /locus_tag="VKO45_08950"
     CDS             complement(5542..>5748)
                     /locus_tag="VKO45_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HMA06038.1"
                     /translation="MVKAKRVYTKDLKRYPEQYKRELALYRKAGTVWISYGVPFILPI
                     LAGFAVALFGGDILFTILTAFAGW"
BASE COUNT          961 a         1877 c         1781 g         1129 t
ORIGIN      
        1 ggggacaatc tgaacgctct cgggggcttc gcccccgccg ctctggcacc cccccctttg
       61 tgatagcccg ttcggaggtt ccctgaagag atcgatcaca gtggagagaa aaacaccgtt
      121 ctaaacaagg gatcagatta cccgggacag gcctatcctc acacgggggg gacgccgaag
      181 cggcgggggt gagcggagcg aactccgcga cttcgtcgct cgagttcggt ttcttacgaa
      241 acctccctcc cccaggagcg tccgccaatc agcgatcaag ttcggatcag aggatagttc
      301 gagagacttg attttgctga atcgactcca ggagcgtcca tgaatcgata gaaaaggatg
      361 tgggaaataa aaaaaaaatt cacccgatca caatcgcggg tttgtagggt aacgcctgtt
      421 tcgccttcgg atgatccgaa tccccggctg agaggatctt caccggtacc ctgaactccc
      481 gcccgaggaa ttccctcgcc gattcgaaga ccgtcatttc atcgaccgga tgggcgagga
      541 gcccctccac ctggacgggc gacaacttgt gcacgagggc cgtgatctgc ttcaccgcct
      601 ccgctgcctc cttccccctt ttccgcatcc cggggtcttt catgatctcc ttcaccacct
      661 cccttctctc gggggaccct gcgatggtcg taaagatgga ccgcttccat tccggggcca
      721 cgaggatctg gatctctttc ggggtgaccg ggaggatctt cgtgatagat ccgatatcct
      781 cgagggtccg ggccaggaga tcctccccga gctcggcatc gatccggatc tttccctcgt
      841 ccgccaccgg ccacggggcg aaggcaacgg gccccttctc cccgagagcc tcccggaggg
      901 cctcgcaggt gtacgggatg aagggggcaa ggagccggac ccaggccgag gcgagggcac
      961 gggcggcggg tcccggggcc gagccctccc ccatcctggt ccgataccat ttcaggtccg
     1021 cctctatccc gtagaagcac tcctgcaggg cctggcgggt ctggaagagc tcgagagcct
     1081 tcgtggcccg ggcgatcctc ccctggagcc ggctctcgag ccaggtgtcg aggggtccgc
     1141 cggcggtgcc cttcgcggcc gcgagctcgt ggatggtccc ccagaacctc tcgatctgct
     1201 ttttcaccga gaggacgagc tcgttccgcc agtcgaagtc ctgccatggc tcggcgctcc
     1261 cggtgaggaa catccggacg gtgtcggccc cgaactcctc gatggcatcc tcgaggagga
     1321 agacgttccc cttggaggag gacatcttcg cccctgaaag gagccccatc ccgaagacca
     1381 ccatcccctt cggctggagc tcgggcggga ggagggcccg gtggtggaag agctggaagg
     1441 tgaggtgatt ggagatcagg tcctttgccg agaaccggaa gtcgtacgga taccagtaga
     1501 ggagctcctt cctgatgggc tcaaggattt cgggagagag ggtggacggt gtgcccttcc
     1561 cgaggaggag gtagtcaaag acttcggggg tgagattctc cggggggagc ttcttcatgt
     1621 ggtgcgcgat ggtgtagtac gccatgtaga tggtggagtc ggagaggggc tccacgatcc
     1681 agttccggtc ccacgggagc tttgtcccga gcccgacctt cctcgtgcag gcccagtcct
     1741 tcagccagtc caccgtccgc tcgaactcgg cccgggtctc aggcggtacg agggccatcc
     1801 tgccgagctg gtcgtggacc tcggccttcc aggccggatc gctgtaggcg aggaaccact
     1861 ggtcatggag gatccggacc atgacccttc ctccgcagcg gcagaccacg gggcgggtgt
     1921 cgaagtcgta catgacggcg gagccgaact cggcgagcat ccggtccgcg acctcgtccc
     1981 tcgcctgtcg tacgcttttc ccgccgaact ccggcctcat cttcccgcgg gcaaactcgg
     2041 cggcatagat ctcctgggtg acggactcga gtttcgggtc cttctggtcc cggatacctg
     2101 ccttctccac cgcctccttt gccgggaact cccccgctcc ctcgaggatg aggaggcttc
     2161 tcggccggat ctcggggtag agcccctgct cctggagatc gcggagggcg atgtagtcgt
     2221 acggggcatg ggcgggaacc gacataacca tcccggatgc catttcggga tcgacaaagg
     2281 gggccgggag gatggggatc tctcccgaga gggggtggga ggccttcttc cccaccagat
     2341 ccctcccccg gacttcggcc ccggtaacca cggtgtggtc ctgcatcttc agcttctcgg
     2401 ccgcctcccg ggagacgatc caggccttcc cgtccacctc ggcccgcaca taggtgacct
     2461 cgggattcac ccagaggttc gtgacgccgt agaccgtttc gggccggagg gtcgctgtcg
     2521 ggatgaaggc atccccgtac cggaacatga cgaggacgaa ccggatgatc tccgccttgt
     2581 ccccctccag gaggtcatgg tccccgacgg ggttgtcgca ctgagggcag aatttcacgg
     2641 ggtgggcccc cttcacgaca tggccctgtt cgtagagatg cccgtactgc cactcgatga
     2701 acttgctgta ctgggggtcg acggtgatga acctcctcct ccagtcgatg gagaggccga
     2761 gggcccgcat gatccgctcg tactcaccgg caaagtaccg gacgatgacc atggggtcgg
     2821 taaaggtgtc gaggacctcc tggggcaccc cgtagaggtc ccggtagagc cagacggcct
     2881 tcgggtcctt tctcgcgatc ctctttgaga tcccgatgac cggcgtcccg gtcacgtgaa
     2941 atcccatggg gaagaggacc tgtctccccc gcatgcgcca gaaccgtgcc acgatgtccg
     3001 gcaccgtata ggtcctgcca tgcccgacat gcatagcccc gctcgggtag ggaaaggcga
     3061 cgttcaggta gtacttctcc ttccccgagg gatttgcctc gaactcggcg gcccagcgat
     3121 cccggtacct ctcctcgagt tcgtggagat cgataccctt tggcatcgga gaccccccta
     3181 taccggcttc acccggatgg tttccttgtt cgtcagccgt cgcatcattt cctgggcgat
     3241 ggtggagttc ctcgcgagcc ggatctcgcc gtcctcgccg acggtcgccg tgaacaggta
     3301 ttccttcccg gcgaagaggt ccacgatctt cccccggtgc tcggtcgcga tgatggagag
     3361 ctgcttccgg tcggtgcgga tccggaaccc gccctcgagc tgcacctcct cctcggagac
     3421 cggtctttcg agctcgctcc ggggccggat gtcgatgccg accgagagct tgttcacgat
     3481 cccggagacg ttctttccgc ccttcccgat ggcggccggg acgtccttgt cctcgatgta
     3541 caccacggcc ttgttgtcgc tgatcatatg gacgtccacg tacccggccg tgtaccgccc
     3601 gatctcccgc tggatctcct tctccaggag attcctcgaa agcctctcct cccccctcgc
     3661 gggcgtcgct ggggcccgtg ccccctcctc gcgcctcgcc ggcataacga tggactcccc
     3721 ctcgtgccgg aacacctcga actcgatggt tcccgtctcc tggttccgga ggagaatgac
     3781 cggccgcggg cgggactcgc ccgccatgcc cgagggcacc tccatcccga actggaggtc
     3841 gtacatcttc gtcacctccc cgtcggccac gaagaagatg gtgttcacca cctgggtgag
     3901 gaccccgaag tccacccggc ccaggacccg ctggagggcg tcctgcacct cgagggcatg
     3961 gacgaccccg atcatgccga cgccggcgag gcgcatgtcc gcgaaggtcg tgaagtcctc
     4021 gttcttccgg agctcgtcaa agatgacgaa gtccggccgc acgaggagga ggacatcgcc
     4081 cgtggactgc atcgatccct cgagagacgt gtactgggtg atgttgtccg ggacctggag
     4141 gtcccggggg gcctccatgg tctttaccac gaggccgtgg tccgagagga aggttgcgat
     4201 ggactgggcg agggtggtct tccccgatcc gggtggcccg gcgatgagga ttccccgccg
     4261 gtccccgatg atcctcttct tgatgagggc cgccttctcg tagttctcca gggtgacctt
     4321 ccccaccggg cgcacggcgg agatctccat cccgtcggag aagggccggc gggcgatggc
     4381 gatgcgaagc gacccgatct ggatcaccgt cgcgccccgc ttctcgagct cgataaagcc
     4441 gtcagggtcc ctcttcgccc gctcgaggat ctcctgcgcc atggctttca gctcgtactc
     4501 ggagaggggg gcgtcccgga tggtgacgag cctgacctcc ttgagggagc ccttctttgc
     4561 caccgggcgc atccgctcct tcaggtacac cgccatggtg ttctcgtcaa agaactggtc
     4621 gatggccagg ggggcgaagt cccccacctg gggcttcagg tagatcacgt cgaggccttt
     4681 cgcccgggcc acctcggcct ggaccacgtc gctcgtgacg aaccgggcct cgtgctcgat
     4741 ggccaccttc cggatcaggg cgtcgatctc cccgccgctc gcgagcttca cctggtccag
     4801 gctcgggcgt tcgcccacgt atttgagctc gatggtcccc tcctttgcca tccgggagag
     4861 ctcctggagc tcggtgaggc ccgagaaacc gatctccctg ccctggtttg cctgggcctc
     4921 gagctcggcc acgaccgcct cggggatgac gatgacggcg ccctggtatt caccggtacg
     4981 tatcatctgg gtgatgcgtc cgtcgataac ggcgctggta tccggtacta tctccataaa
     5041 agccacgcct ccagatcttg cgatgatatc ctattctacc gaaagatgtt gcatgcaggg
     5101 ggtcatggat gtttttattc tgatccggtg ggaggcgggg aaatctccgg ggtcagtcct
     5161 ttttctcctt cttcctgtac acctcgtccg ggtcgaacat ccggggggtg atctcctcgc
     5221 ccgccagagt gcggtagaaa catgaccggt ggccggtgtg gcaggcgacg cccatctggt
     5281 cgacctcgta gagcagggtg tcctcgtcgc agtccacgag gatccgcacg atcctctgga
     5341 ggtggccgct ctcctcgccc ttcttccaga tccggccccg ggaccggctg aagtagtggg
     5401 cgagccccgt cttccgggtc aggtccaggg cctcccggtt cgcgtacgcc atcatgagaa
     5461 cttcccgggt ccgggtatcg acgacaacga ccgggatgag gccatcggta aatttcaggt
     5521 cgatcatggc atctcccctc ctcaccaacc cgcaaaggcg gtcaggatgg tgaagaggat
     5581 gtcacctccg aacagcgcca cggcgaaccc ggccaggatg ggaagaatga aggggacacc
     5641 ataggagatc cagacggttc ccgcctttcg gtacaaagcg agctccctct tgtactgctc
     5701 cgggtacctc ttcaggtctt tggtgtacac ccgcttcgcc ttcaccat
//
LOCUS       DATGDI010000238         7103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_200634, whole genome shotgun sequence.
ACCESSION   DATGDI010000238 DATGDI010000000
VERSION     DATGDI010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200634"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VKO45_08955"
     CDS             <1..205
                     /locus_tag="VKO45_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06039.1"
                     /translation="GTRLMEAVAAIAVKGNRRILLTAEKDAKGFFRAMGMVLREEYPD
                     GNGVFELAGDGLRRLAAGSPGNR"
     gene            complement(268..612)
                     /locus_tag="VKO45_08960"
     CDS             complement(268..612)
                     /locus_tag="VKO45_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06040.1"
                     /translation="MGPLTVICILGLVVGTASALFITATDAGAAPASGQVGNPHHPDL
                     INVTREQGILIQIEDTGADVTGSNAHPLSGDVVAMEIWPGCSVAGELPPDLSCGLIPV
                     SGWPQNVTGNNG"
     gene            913..1173
                     /locus_tag="VKO45_08965"
     CDS             913..1173
                     /locus_tag="VKO45_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMA06041.1"
                     /translation="MPDEKHENLHLEVELPGVERQSITLSMHADSFFIRASREDLEFV
                     GSYAVCCPIDFEKAKAKYNNGLLSIDVPYKKPQERGKKIAVQ"
     gene            complement(1204..1602)
                     /locus_tag="VKO45_08970"
     CDS             complement(1204..1602)
                     /locus_tag="VKO45_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06042.1"
                     /translation="MVKKVPIETKFLKEIIKDLSCEDPEARRKALDAVMTAAWQPGWK
                     PAEFIALGGLPALIGRLSEPDEKSRVQAVEAVERMSELGASSELAGQGVIPLLERMAS
                     ADRYEPLRMIAGRAVAKIRGRMKVQTQDTR"
     gene            complement(1810..1883)
                     /locus_tag="VKO45_08975"
     tRNA            complement(1810..1883)
                     /locus_tag="VKO45_08975"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1847..1849),aa:Val,seq:gac)
     gene            complement(1969..3555)
                     /gene="serA"
                     /locus_tag="VKO45_08980"
     CDS             complement(1969..3555)
                     /gene="serA"
                     /locus_tag="VKO45_08980"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HMA06043.1"
                     /translation="MKLKVLVSDPLAEEGLAILRERCEVDVRTDLKEDEIVRIIGDYD
                     ALVVRSGTTVTARIIDAGKRMKIIGRAGVGVDNIDVEAATRRGIPVVNSPEGNTLAAT
                     EHTMAMMLALARNIPQATASLKQKQWKRSKFMGIELNEKTLGILGLGRIGREIAKRAK
                     AMEMRVIAYDPFITKERAAQMGVELMALDDVIRAADVITVHTPLTPETTHLINAQKFA
                     LMKKGVRVINCARGGIIDEKALYDAIRSGKVAGAALDVFEDEPPLSSPLLDLENVIVT
                     PHLGASTVEAQLNVAVSVAHQVVGFFGGEQPKYAVNAPMVPAEYADRMEPFATLTEKM
                     GRFLIQLAEGRLEAMEITYGGELSSLGPNTKFLTRIALKGLMDPILQIPVNVINAEFI
                     AKERGIAIKEALTDDAAGFRNLITIRVKTDKMEETMAGTVFTRDRIRIVAIGGYTMDM
                     IPEGFVIVSRHLDKPGVIGRASTILGEAKINIAGMQVGRIKQGEEAIMVLNVDSEVPP
                     AVMEEIREKVGIFTAKFAKI"
     gene            3791..6070
                     /gene="ppsA"
                     /locus_tag="VKO45_08985"
     CDS             3791..6070
                     /gene="ppsA"
                     /locus_tag="VKO45_08985"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HMA06044.1"
                     /translation="MKKVPDILTLEEIKKEDIPSVGGKGASLGEMFGIGLPVPRAFVV
                     TAQAFRRFLQETGIEDRLFAGLAHLNVEDNAALEGASAKARELVLSVAIPQHLQKEIR
                     EAYRALGSEPVVAVRSSATAEDLPDASFAGQQETYLNIKGNGDLIRSVQKCWASLYGA
                     RAIYYRAKQGFDDRSVNIAVVVQELVPSEKAGVMFTSHPVTGEPLTIIEGSWGLGEAV
                     VSGSVSPDKYVYDHRSEQVVDRLISQKLEMVVPEGKKGTKTVKVPPDQEKAPVLADGE
                     VKRLAEFGKIAEDHYGIPQDLEWAITGGEFFILQSRPITTIKAPVQEKPATAAAGKVL
                     LEGQGASPGIGSGRVAIVRDIKDTGNVKEGDILVAKMTNPDMVPAMRKVAAIVTDEGG
                     MTCHAAIVSRELGTPAVVGTKKATSILRPGQVVTVDGEKGLVYEGKIGAPAPKEKAVA
                     AVMAAPLITATSVKVNVSLPEAAARAAATGADGVGLLRIEHLILGLNKTPMWYIRNRK
                     EEEFIGELAGGIRVVLDAFPGKPVWVRTLDAPTDEFRNMAGGEDEPHEHNPMLGWRGI
                     RRDLQSTDQFRLQVEAFKRLWQAGYTNLGMMFPMVSHPDEFILAKEMMRGWGVDVENV
                     TLGIMVEIPASAILIEDFIRAGIRFASFGTNDLIQYTIAVDRNNENVASMYRPKHPAV
                     LKIIDDTIRVCRRYGVECSICGQAGSDPEMAEWLVEHGISSLSANIDAVAKIRDRVAR
                     TEQRMILEAARRMHAREGV"
     gene            6051..>7103
                     /gene="mfnA"
                     /locus_tag="VKO45_08990"
     CDS             6051..>7103
                     /gene="mfnA"
                     /locus_tag="VKO45_08990"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase MfnA"
                     /protein_id="HMA06045.1"
                     /translation="MREKGCSREELFSFLTRKREEDREYGRILSSMCTRPHPVALEAE
                     ALFAGTNLGDPGLFPGTAGIERLLISRLGDLLHHKEACGYATSGGTESNLQVLRMARG
                     LAGVSDPNVVVPESAHFSFEKACDILSIGIRTVPLDGNLRMDPGQAEEQMDRNTICLV
                     GIAGTTEYGMVDPIRALSDIAMDRGVFLHVDAAFGGLVIPFLEKPFPFDFSLPGVSSI
                     SVDPHKMGMSPIPAGALLVRDERMLAPLSVATPYLTVRNVYTLTGTRPGGPVAGAFAV
                     LEYLGREGMRSIVRGCMENTARLIAGLETFGIPRATTPDVNVATFICDRTPEGWQVSR
                     TRRGHLRIVCMPHVSRD"
BASE COUNT         1298 a         2282 c         2285 g         1238 t
ORIGIN      
        1 cggcacccgg ctcatggagg cggtcgcggc catagccgtc aagggaaacc ggaggatcct
       61 gctcacggcg gagaaggatg cgaagggctt cttcagagcc atggggatgg tgctgcggga
      121 ggagtacccg gacggaaacg gcgtgttcga gctcgcagga gatggtttga gacggctcgc
      181 cgcaggatct ccggggaatc gatgaagagc ctggggtcgg tccgaatcct tgaaaatgaa
      241 aaaaggttgg gaatcccgcc gggaggatta cccgttgttc ccggtgacat tttgcggcca
      301 cccggaaacc gggatcagtc cgcatgagag gtcgggaggc agctctcccg ccactgaaca
      361 cccgggccaa atctccatag ccacaacatc tccgctcaac gggtgggcat tgctaccggt
      421 gacatcagct cccgtgtcct cgatctggat caggattccc tgctcccggg tgacattgat
      481 caggtcgggg tggtgtggat ttccgacctg acccgacgca ggagcggcgc cggcatcggt
      541 tgcggtgatg aacagggcgc tcgctgttcc cacgacgagc ccgaggatgc agatgaccgt
      601 gaggggtccc atgacaaatc cttgcctgtg catggcagat ccttcctgtt caccggtggg
      661 atcccggggg aaacccgggc cacaccaatg taacctttat tttacattta gtaatatata
      721 actttctact catcctgttt atagcacaat tccggaatcg acaccccggt ccggccctga
      781 ccggcatgcc acgggtattt ctcccggtga aacgaggggg aacccttaag tatctcaccg
      841 ccaccagtcg gatgaacagg aggtatcgaa ggaatgtcaa aaaagaccag gatgtcgccg
      901 gccatatgcg ccatgcccga tgaaaaacat gaaaatctcc atctcgaggt ggagctcccg
      961 ggagtggaga ggcagtccat caccctctcc atgcacgcgg acagcttctt tatccgtgca
     1021 tcccgggaag acctggagtt cgttggctcc tatgccgtct gttgccccat cgatttcgaa
     1081 aaggcgaagg cgaagtacaa caacgggctt ctttccatcg atgtcccgta caaaaagccc
     1141 caggaacggg ggaaaaagat agcggtacag tgagaagcca gaccggctct caccgtcccg
     1201 cgcttaccgg gtgtcctgcg tctgaacctt catccggccc cggatcttcg caaccgctct
     1261 ccctgctatc atccggaggg gctcgtacct gtcggccgag gccatgcgct cgaggagggg
     1321 gatcacgccc tgccccgcga gctcggacga ggccccgagt tccgacatcc gctcaacggc
     1381 ctccaccgcc tggaccctgc tcttctcgtc cggctccgag agccgcccga tgagggccgg
     1441 gaggcccccg agggcgataa actctgccgg tttccacccg ggctgccagg cggccgtcat
     1501 gaccgcgtcg agggccttcc tccgtgcctc tggatcctcg caggagagat ccttgatgat
     1561 ctccttcagg aacttcgtct caatggggac cttcttcacc atgggatgaa acactccttc
     1621 tcgcctaccg ctcccctttc gcgggggcat ataaaaggat tacgggcaag aagatctttt
     1681 gaaacgtccg aggggatgca aatccccgag gacgggagca gccatcagga tgcgacaccg
     1741 aggggatgca aatccccgag gacgggagca gccatcagga tgcgacgtct aaaaaaagaa
     1801 atgaaaagat gggcccgctg agattcgaac tcaggatctc cgccatgtca aggcgacgtc
     1861 atagccgact agaccacgag cccaaagaga cgtctatact gttggttccc ccccgatata
     1921 aaagagtatg gaagagggca gggaatcgcc ttcccgcccc gatctgcctt aaatcttcgc
     1981 gaacttggcg gtgaagatcc cgaccttctc ccggatctcc tccattaccg ccgggggcac
     2041 ctcggagtcc acgttcagga ccatgatggc ctcctcgccc tgcttgatcc ggcccacctg
     2101 catgccggcg atgttgatct tcgcctcccc gaggatggtg gaggcccggc cgatgacccc
     2161 gggcttgtcc aggtgccggg agacgatgac gaagccctcg gggatcatgt ccatggtata
     2221 gcctccgatg gcgacgatcc ggatccggtc cctcgtgaag accgtgccgg ccatggtctc
     2281 ctccattttg tccgtcttca cccggatggt gatgaggttc cggaatcccg cagcatcgtc
     2341 ggtgagagcc tccttgatgg cgattccccg ctcctttgcg atgaactcgg cgttgatgac
     2401 attcaccggg atctggagga taggatccat gaggcccttg agggcgatcc gggtgaggaa
     2461 cttggtgttc ggtccgaggg aggagagctc gcccccatag gtgatctcca tggcctcgag
     2521 ccttccctcc gcgagctgga tcaggaaccg gcccatcttc tcggtgaggg tggcgaaggg
     2581 ctccatccgg tcggcatact ccgccggcac catgggggcg ttcaccgcgt acttcggctg
     2641 ctcgccgccg aagaaaccga ccacctggtg ggcgaccgat accgcgacgt tcagctgcgc
     2701 ctcgacggtg cttgccccca ggtgcggggt cacgatgacg ttctcgaggt cgaggagggg
     2761 ggacgagagt gggggctcgt cctcgaagac atcgagggcc gcaccggcga ccttcccgct
     2821 cctgatggcg tcgtagaggg ccttctcgtc gatgatcccg ccccgggcgc agttgatgac
     2881 ccggacgccc ttcttcatga gggcgaattt ctgtgcattg atgaggtggg tggtctcggg
     2941 agtgaggggg gtgtggacgg tgatcacgtc ggccgcccgg atgacgtcgt ccagggccat
     3001 gagctcgact cccatctggg ccgcccgttc cttcgtgatg aaggggtcat aggcgatgac
     3061 ccgcatctcc atggccttcg cccgcttcgc gatctcccgg ccgatccggc cgagccccag
     3121 gatgccgagg gtcttctcgt tcagctcgat ccccatgaac ttcgagcgct tccactgctt
     3181 ctgcttcagg gatgccgtgg cctgggggat gttccgggcg agggcgagca tcatggccat
     3241 ggtgtgctcg gtggcggcga gggtgttccc ctcgggggag ttcaccacgg ggatcccccg
     3301 ccgggtcgcg gcctcgacgt cgatgttgtc cacgccgaca cccgcccggc cgatgatctt
     3361 catccgcttc cctgcgtcga tgatccgggc ggtgaccgtt gtcccgctcc ggacgacgag
     3421 ggcgtcgtaa tccccgatga tccggacgat ctcgtcctcc ttaagatcgg tgcggacgtc
     3481 cacctcgcac cgctcccgga ggatcgcgag gccctcctcg gccaggggat cgctcacgag
     3541 caccttgagc ttcactggta aaacccatct cacatcgccc cggtgcctat tttagctttc
     3601 caaacaggac gcccgggtca aaagccggtg cctgtcgatg gagaggggaa gggagcaccg
     3661 gagggcgatc cggggtgccg gacggttcct ccggtcccct gaaccggcat ccttctcggg
     3721 tgtaacagtc atcctttatc tgcagggagc acaatctcgt atcagaaccc gtgacgtggg
     3781 gtactatgcc atgaagaagg tccctgacat cctgaccctg gaagagatca agaaggagga
     3841 catcccctcg gtgggaggaa agggggcatc cctcggcgag atgttcggca tcggcctccc
     3901 ggttccccgg gccttcgtgg tgacggccca ggccttccgg aggttcctcc aggagaccgg
     3961 catcgaggac cggctctttg cggggcttgc ccacctgaat gtcgaggaca acgcggctct
     4021 cgagggggcc tccgcgaagg cacgcgagct cgtcctctcg gtcgccatcc cccagcacct
     4081 ccagaaggag atccgcgagg cctaccgggc cctcgggagc gaaccggtcg tcgcggtccg
     4141 ctcgagcgcc acggccgagg acctgccgga cgcctccttc gcgggccagc aggagaccta
     4201 cctgaacatc aaggggaacg gggatctcat ccggtcggtc cagaagtgct gggcctccct
     4261 ctacggggcg agggccatct actaccgggc gaagcagggc ttcgatgacc ggagcgtgaa
     4321 catcgccgtg gtggtccagg aactcgtgcc gtccgagaag gccggcgtca tgttcacctc
     4381 ccacccggtc accggcgagc ccctcaccat catcgagggg tcctggggcc tcggggaggc
     4441 ggtggtctcg ggatcggtct cgccagacaa gtacgtctac gaccaccggt ccgaacaggt
     4501 cgtggaccgg ctgatctccc agaagctcga gatggtcgtt cccgaaggga aaaaggggac
     4561 gaagaccgtg aaagtccccc ctgaccagga gaaggccccc gtgctcgccg acggcgaggt
     4621 gaagaggctc gcggaattcg ggaagatcgc ggaggaccac tacgggatac cccaggacct
     4681 cgagtgggcc atcacgggag gggagttttt catcctccag tcccggccca tcaccaccat
     4741 aaaggccccg gtccaggaaa agcctgccac ggcggccgcc gggaaggtcc tcctcgaggg
     4801 gcagggggcc tcgcccggga tcggatcggg ccgggtcgcc atcgtccggg acatcaagga
     4861 caccgggaac gtgaaggagg gggacatcct cgtcgcaaaa atgacgaacc cggacatggt
     4921 cccggcgatg cgcaaggtcg cggccatcgt caccgacgag ggcgggatga cctgccacgc
     4981 ggccatcgtc tcccgggagc tcgggacccc cgcggtcgtc gggacgaaga aggccacctc
     5041 gatcctccgt cccggccagg tggtgaccgt cgacggggag aagggcctcg tctacgaggg
     5101 aaagatcggg gctccggcgc cgaaggagaa ggccgtggcg gccgtcatgg cggcccccct
     5161 cattaccgcc acctcggtga aggtgaacgt ctctctcccc gaggcggccg cccgggccgc
     5221 cgcgacgggg gcggacgggg tgggcctcct ccggatcgag cacctcatcc tcggcctgaa
     5281 caagaccccc atgtggtaca tccggaacag gaaggaggag gagttcatcg gcgagctggc
     5341 gggcgggatc cgggtcgtcc tcgatgcctt cccggggaag ccggtctggg tccggaccct
     5401 cgatgccccc accgacgagt tccggaacat ggccggcgga gaggacgaac cccacgagca
     5461 caacccgatg ctcgggtggc gggggatccg ccgggacctc cagagcaccg accagttccg
     5521 gctccaggtc gaggccttca aacgtctctg gcaggcgggc tacacgaacc tggggatgat
     5581 gtttcccatg gtttcccacc ccgacgagtt catcctcgcc aaggagatga tgcgggggtg
     5641 gggggtggac gtggagaacg tcaccctcgg gatcatggtg gagatccccg cgagcgccat
     5701 cctcatcgag gacttcatca gggcggggat ccggttcgcc tcctttggca cgaatgacct
     5761 gatccagtac accatcgctg tcgaccggaa caatgagaac gtcgccagca tgtaccggcc
     5821 caagcatccc gcggtactca agatcatcga cgataccatc agggtctgcc ggcggtacgg
     5881 cgtggaatgt tccatctgcg gccaggcggg ctccgacccc gagatggctg agtggctcgt
     5941 cgagcacggg atctcgagcc tctcggccaa catcgacgcc gtggcgaaga tccgggaccg
     6001 ggtggcccgc accgagcagc ggatgatcct cgaggcggcg agaaggatgc atgcgcgaga
     6061 aggggtgtag ccgggaggag ctcttctcct tcctcacccg gaaaagagaa gaggatcggg
     6121 agtacgggag gatcctctcc tccatgtgca cccggcccca cccggtcgcc ctcgaggccg
     6181 aggccctctt cgcgggcacg aacctgggcg atcccggcct cttcccgggg acggccggga
     6241 tcgaacggct cctcatcagc cggctcgggg atctcctcca ccacaaggag gcctgcgggt
     6301 acgcaacttc gggggggacc gagtccaacc tccaggtcct ccggatggcc cggggcctcg
     6361 ccggggtgag cgaccccaac gtggtcgtgc cggagtctgc ccatttctcc ttcgagaagg
     6421 cctgcgacat cctctccatc gggatccgca ccgtgcccct ggacgggaat ttgagaatgg
     6481 atcccgggca ggccgaggag cagatggacc ggaacaccat ctgtctcgtc gggatcgcgg
     6541 gcaccaccga gtacgggatg gtcgacccca tccgggccct ctcggatatc gccatggacc
     6601 gcggggtctt cctccatgtc gatgcggcct tcgggggcct cgtcatcccg tttctcgaga
     6661 agcccttccc cttcgacttc tccctcccgg gggtctcctc catctcggtg gatccccaca
     6721 agatggggat gtcccctatc ccggcgggcg ccctcctcgt cagggacgag aggatgctcg
     6781 cccccctctc ggtggcgacg ccatacctga cagtgcggaa cgtgtacacc ctcaccggga
     6841 cccggcccgg gggaccggtc gccggggcct tcgccgtcct cgagtacctc gggagggagg
     6901 ggatgcgctc catcgtgcgg gggtgcatgg agaacacggc ccggctcatc gccggcctcg
     6961 agaccttcgg gatcccgagg gcgacgaccc ccgacgtgaa cgtggccacc ttcatctgcg
     7021 accggacccc ggaggggtgg caggtctccc ggacccggag gggtcacctc cggatcgtct
     7081 gcatgcccca cgtgagccgg gac
//
LOCUS       DATGDI010000239         8955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_259379, whole genome shotgun sequence.
ACCESSION   DATGDI010000239 DATGDI010000000
VERSION     DATGDI010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 8955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8955
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259379"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VKO45_08995"
     CDS             complement(<1..671)
                     /locus_tag="VKO45_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HMA06046.1"
                     /translation="MPSPLTSLDFFRDSYDTRIAQLREERAAGMKIVGTFCLYVPDEI
                     ILAAGADRVVLCGGAADTIPVAEQSLPRNLCPLVKSSFGAVVDACCGGNLACPHVGLV
                     DVVVAEATCDGKKKMYEVLSSHIPTYVLDLPQKPDSPEALAYFGSELRRFGRFMEGLT
                     GRTITDDALAREIRSANETRRLLHRLFGMRMADPPPIRGSEVLRVMQKQFFLSPVQFR
                     EGITRL"
     gene            824..1129
                     /locus_tag="VKO45_09000"
     CDS             824..1129
                     /locus_tag="VKO45_09000"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HMA06047.1"
                     /translation="MAKIQVGLSKDITDGAMKGFRAGDRPVLVSNIDGKLYAIGDKCT
                     HRGCSLSKGRKEGDVVTCPCHGSRFNLKTGAVVRGPAENPEPAYPVTLENDVIWVEV"
     gene            complement(1208..1281)
                     /locus_tag="VKO45_09005"
     tRNA            complement(1208..1281)
                     /locus_tag="VKO45_09005"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1245..1247),aa:Phe,seq:gaa)
     gene            1401..2372
                     /locus_tag="VKO45_09010"
     CDS             1401..2372
                     /locus_tag="VKO45_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HMA06048.1"
                     /translation="MGRPGLKGRYSADELKEAIRSRRAKVVHLTHNDLDAAGADAIHR
                     MKYGEVFTIWASVGNFPHLLSAISEVKGGGDLLSITDLGYQAGIERWLKKARDAGWRI
                     EWRDHHRWEDREAGIIRPLAALLSLDTATCATGIVARDLAGSDSRAAEIARVVCDYDL
                     WRNEDPRSMILGRILWKPGLRDYVRDRLVEGVFADALIREEYDKIVAEMDQDIRKSIR
                     YTRLTGKRYRIAFSPLYGYPSDTASRVRESFGSEIEVLVSSNGRFSLRSVPPVSHLIA
                     RRFGGGGHPHASGGAFPFTLLDRVTFVLFKRSRHFEDLVAVAESIGE"
     gene            complement(2359..3144)
                     /locus_tag="VKO45_09015"
     CDS             complement(2359..3144)
                     /locus_tag="VKO45_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06049.1"
                     /translation="MAGARKVPVRSFGSEVPDPTVEELGEWIGTRKGTGGDLTSFMLE
                     RSLESQMEVDLPCPGGRFYRARILESLRGLEGETLVREPSADTALVEEDARWGASRKK
                     GLRFSLPAPHLLGIRDGYYHHREEFCEGICTCYRQIQRAMRDTGAVGHVLLGERVFEE
                     EMEHLAGPRTLFFFPDLQEGDLPAILEFQDVAAVPRALLPRTLELLDEFDLRHVTIVD
                     GDKEDLVAALEQLDPDQISLGGYCGEDCGEYWKRLVERAVIPR"
     gene            3189..5180
                     /gene="metG"
                     /locus_tag="VKO45_09020"
     CDS             3189..5180
                     /gene="metG"
                     /locus_tag="VKO45_09020"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HMA06050.1"
                     /translation="MSDGPILVTCGLPYTNGPCHLGHLRTYVPADFYVRFLRRRGEEV
                     LFVSGSDNHGTPIVVMAEDQGVSPRDLSNRYHEHFAHIFQRMGIRFDHFGMTDGETNH
                     RRTRSIVNALKEKGYIYSQVVEQSYCPKCKRFLPDRYVEGICPYCDTPARGDECDQGC
                     GRHLEPGEIRKPRCLVCGTDAELREQEHFFFRLSEFSGFLEEYLPGLKGTANARNYAL
                     GWVKEGLRDWNITRTLDWGVKFPGHDDLVVYVWVDAPIGYISFTEEWARARGLDWKRY
                     WCGKNQVTHFIGSDIIYHHCIFWPALLKGAGYSPPLAVVASGMVKIDDQKFSKSRGYV
                     VWTGEDYLDKGLPPDYLRYYLLSYTNHTKELNFSWKVFSERVNNELVSTLGNFIYRSL
                     FFAHKFFGRVPEGPVGSGTSEAIGRCVEDMDKAVREFEFKGATDALMALAAYGNTYIQ
                     NQAPWKLVKTDQAAAERVVKDCLQIAHALALLMEPILPDAAGRVWRMLGNTDAVETHP
                     VLDATRQIPSVALPEPAPLFEKLEEGRIRELEKILKMRTEKAKTAVKEKDLISIEEFS
                     RMDIRVGKILSAEPVKGSKKLLRLSVDIGEEKSRLVVAGMAGIHRPEEMVGLSVIMVT
                     NMKPAKIFGIESQGMVLAAGEEASLLVPLKEVPPGTKIR"
     gene            5222..5881
                     /locus_tag="VKO45_09025"
     CDS             5222..5881
                     /locus_tag="VKO45_09025"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HMA06051.1"
                     /translation="MVETDGFLFESWAPGKETLKAFSDGIFTVAVTLLVLEIRLPDIS
                     SGEIDARFLESLAAIAPKMLGFILSFFIIAMYWLLYHRLFRYIRETDRSLVGLNILFL
                     FFIVLMPFPTYLLGLYGDHRSIVMFYAGIVAVTSAILAFMWRYASVEHRLVGPDLDDR
                     VIRLMWMRTLIPVGVFSLSILVAIASPFVAMVMWVLNFFIILGVTRFYRPDNSKQPET
                     R"
     gene            complement(5892..6341)
                     /locus_tag="VKO45_09030"
     CDS             complement(5892..6341)
                     /locus_tag="VKO45_09030"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMA06052.1"
                     /translation="MKGLVARQSVSIRAPPAEVWKALTDPDLIRQYLFGTEATSDWKA
                     GSQIRYRGTWEGKAYEDKGVVLRAEPGKMLETTYWSSMSGLPDIPGNYRLVRYELTPE
                     KGGGTLLTVTQDNNPTEEGRAHAEGNWKVVLEGLKRLLETGRSPGST"
     gene            complement(6338..7120)
                     /locus_tag="VKO45_09035"
     CDS             complement(6338..7120)
                     /locus_tag="VKO45_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HMA06053.1"
                     /translation="MSDSEGETGKETPGKGQKNTGLPEHRIHVCKNGPYQVFGGIPLS
                     VQRITLDEAGYSSVWKEEKKFEVENPYILCRCGRSRTLPFCDQYHKEIHFIGTEHASR
                     EPYLKQATEYGGPNLVLTDAEILCSVAKFCERAGGIWKLTQESGNREAREIAIQEAGN
                     CPSGRLVVWEKGTRIPIEPHFEHSIGLVEYPQEGLSGPVWVRGGIPVISHDGWVHEIR
                     NRVTLCRCGHSFNKPFCDSSHLRFSSRDIMLKIPRTRPTSRP"
     gene            complement(7286..7360)
                     /locus_tag="VKO45_09040"
     tRNA            complement(7286..7360)
                     /locus_tag="VKO45_09040"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7323..7325),aa:Arg,seq:tct)
     gene            complement(7426..7818)
                     /locus_tag="VKO45_09045"
     CDS             complement(7426..7818)
                     /locus_tag="VKO45_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF473 domain-containing protein"
                     /protein_id="HMA06054.1"
                     /translation="MSFAALTGISPAILADLKQGRPRTVELTSAHNIITLSGVKAGDH
                     VFITSLDCADLGPGDSGIMVEVNSVAISMKRIIEFTNPMFFEERERMAARMQVKYCGT
                     SVVKHIESRGMGRPVLVDVVKTTCYHAH"
     gene            complement(7824..8537)
                     /locus_tag="VKO45_09050"
     CDS             complement(7824..8537)
                     /locus_tag="VKO45_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HMA06055.1"
                     /translation="MEEIRIVSRPLADTTKTVLIGFPGSGLVGSIALQYLVDNLEFPQ
                     IGSVTSKYFPPLAMMTKGVINVPVRFYEKNDIAVVVGDIPIHPMICYEVACGLLDWFA
                     PFKPKEVLTIAGIVTNEPEKRVFGVATNPDALQRIQDQTMILPIGSISGIAGSILTEC
                     KIRDIRGLGLLGETVNNPDPRSSAATIQVLNKMYDLKLDVGPLMEQAEEIEAAMHRIA
                     EEVQTTEAGQKKEQLPMYG"
     gene            8585..8908
                     /locus_tag="VKO45_09055"
     CDS             8585..8908
                     /locus_tag="VKO45_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5611 family protein"
                     /protein_id="HMA06056.1"
                     /translation="MQEFQVKRGHTKDLAGEVEKAFTDCFGVRPEKNGETYAISYGAL
                     SRMEVQIGGGGKTLTVNTVSRKDAGEAAILDTNRRFRTFLDRVTGFSSKERVKRAKKA
                     VEESG"
BASE COUNT         1696 a         2691 c         2802 g         1766 t
ORIGIN      
        1 agccgggtta tcccctcccg gaactgcacg ggggagagga agaactgctt ctgcatcacc
       61 cggaggacct ctgagccccg gatgggggga gggtctgcca tcctcatgcc gaacagccgg
      121 tggaggagcc tccgggtctc gttcgccgat cggatctctc ttgccagggc atcgtcggtg
      181 atggtcctcc cggtcaggcc ttccatgaac cgtccgaacc tccggagctc cgagccgaag
      241 taggccaggg cctcgggact gtcgggtttc tgcgggagat cgaggacgta ggtggggata
      301 tgggaggaga ggacctcgta catcttcttc ttcccgtcgc aggtcgcctc tgccaccacc
      361 acatccacga gcccgacatg ggggcaggcg aggtttcccc cgcagcaggc atcgaccacg
      421 gcaccgaagg aggacttgac gagcgggcag aggttcctcg gcagggactg ctctgccacg
      481 ggaatcgtat ccgcggcccc cccgcagagg acgacccggt ccgcccccgc cgcgaggatg
      541 atctcgtcgg ggacatagag gcagaaggtc ccgacgatct tcatcccggc cgcccgctcc
      601 tccctcagct gggcgatgcg ggtatcgtag gaatctctga aaaagtcgag gctcgtgagc
      661 ggggatggca tgggttcctg gtactagggt ataggcacgg atctcttcaa atatcgtgtc
      721 atcatgcgcc ggttcccgcc tccggggacc cttgcaaaaa ctggaagctg gcgaaaccct
      781 tatcaatcct gatggggaga ttgcggcagg gactgatgga ataatggcga agatacaggt
      841 tggcctctca aaagacatta cagacggagc gatgaagggt ttcagggcgg gggacaggcc
      901 cgtcctcgtt tcaaacatcg acgggaaact ctatgccatc ggcgacaagt gcacccaccg
      961 gggatgctcc ctctcgaagg ggaggaagga gggggatgtc gtcacctgtc cctgccatgg
     1021 atcgaggttc aacctgaaga cgggtgccgt cgtgaggggc ccggcagaga acccggaacc
     1081 ggcctacccg gtcaccctcg agaatgacgt gatctgggtc gaggtgtagc acggagccgg
     1141 ctatcggcct catgggagcg aatattttcc tcgggctctc gatgatgcac gtgatctgaa
     1201 aaagggatgc ctcagcccgg atttgaaccg gggacaacca gatcttcagt ctggcgctct
     1261 cccaagctga gctactaagg cgatgaccgt acgtatcttg cgtatgaata ttaaatcatc
     1321 tggtttttcc ggttgcctgc catgaggcgg ccggtgtgaa aggtcagccc tttaataggg
     1381 tgccgggcaa cggtacaccg atgggaagac cggggctgaa ggggaggtat tcggcggacg
     1441 agctgaagga agctatccgg tcgagaaggg cgaaggtggt ccacctcacc cacaacgacc
     1501 tggatgcagc gggggccgat gccattcacc ggatgaaata cggcgaggtc ttcaccatct
     1561 gggcctccgt cgggaacttc ccccacctcc tctctgccat ttccgaggtg aagggaggcg
     1621 gggatctgct ctccatcacc gatctcgggt accaggccgg catcgagcgg tggctgaaga
     1681 aggcaaggga tgccggctgg aggatcgagt ggagggacca ccaccggtgg gaggaccggg
     1741 aggccgggat catccgcccc ctggcggccc tcctctcgct ggataccgcc acctgtgcga
     1801 cgggcatcgt cgcccgggat cttgccggtt cggattcccg ggcggcggag atcgcccggg
     1861 tggtctgcga ctatgacctc tggcggaacg aggacccgag gagcatgatc ctcggacgga
     1921 tcctctggaa gccgggcctg cgcgactatg tcagggaccg gctcgtcgag ggggtgtttg
     1981 ccgatgccct catcagggag gaatatgaca aaatcgtcgc ggagatggac caggatatca
     2041 ggaagagcat ccggtacacc aggctcacgg gaaagaggta ccgcatcgcc ttctcccccc
     2101 tctacgggta cccgagcgat accgcctccc gggtgaggga gtctttcggg tcagagatcg
     2161 aggtcctcgt ctccagcaat ggccggttct ccctccggtc ggtcccgccg gtgagccacc
     2221 tcatcgcaag gaggttcggc gggggcgggc atccccatgc gtcgggaggg gcattcccct
     2281 tcaccctcct ggatcgggtc accttcgtcc tcttcaagag gagccggcac ttcgaggatc
     2341 tcgtggccgt ggccgagtct atcggggaat gaccgccctt tccacgagcc tcttccagta
     2401 ctctccacag tcttccccgc aataaccacc cagggagatc tggtcgggat cgagctgctc
     2461 aagggccgcg acaagatctt ctttgtcacc gtcgacgatg gtgacgtggc gcagatcgaa
     2521 ttcatcgagg agctcaaggg tcctcggtaa cagggcccgg ggaaccgccg cgacatcctg
     2581 aaattcaagg atggccggga gatccccttc ctggagatcg gggaagaaga agagggtccg
     2641 cggcccggcg aggtgctcca tctcctcctc gaacaccctt tctccgagaa gaacatgccc
     2701 gacggccccg gtatcccgca tggcccgctg gatctgccgg tagcaggtgc agataccctc
     2761 gcagaactcc tcacggtgat gataataccc gtccctgatc ccgaggaggt gaggtgcggg
     2821 aagggagaac cggagcccct ttttccggga ggccccccac cgggcatcct cctcgacgag
     2881 ggcggtgtct gcagaaggtt cacggacgag ggtttccccc tcgagacccc ggagggattc
     2941 caggatccgg gcacggtaga aacgcccgcc ggggcagggc agatccactt ccatctggga
     3001 ttccagggac cgctcgagca tgaacgaggt aagatcccct ccggttccct ttcgtgtccc
     3061 gatccattcg cccagttcct ctacagtcgg atccggcacc tcggacccga aggaccggac
     3121 cggaaccttc ctcgcccctg ccataccctg tatcctattt atagcgggga gcatcaaaaa
     3181 gcatagagat gagtgatgga cccattctcg taacctgcgg gcttccctac acgaacggtc
     3241 cctgccatct cgggcacctt cggacctatg tcccggcgga cttctatgtc cggttcctgc
     3301 gccggagagg agaggaggtg ctcttcgtca gcggctccga caaccacggc acccccatcg
     3361 tcgtcatggc cgaggaccag ggagtgtccc cccgggacct ctccaaccgg taccacgagc
     3421 acttcgccca catcttccag cggatgggca tccgcttcga tcacttcggc atgacggacg
     3481 gggagacgaa ccaccgcagg accaggtcca tcgtgaatgc cctcaaggag aagggctaca
     3541 tctactcgca ggtggtcgag cagagctact gcccgaagtg caagcggttc cttccggacc
     3601 ggtacgtcga gggcatctgt ccctattgcg acacgccggc gcggggggac gagtgcgacc
     3661 agggatgcgg gaggcacctc gagccggggg agatcaggaa gccccggtgc ctggtctgcg
     3721 ggaccgatgc cgagctcagg gagcaggagc atttcttctt ccggctctcg gagttcagcg
     3781 gcttcctcga ggaatacctc ccgggtctca aggggaccgc aaacgccagg aactatgccc
     3841 tcggctgggt gaaggagggc ctccgggact ggaatatcac ccgcaccctg gactgggggg
     3901 tgaagttccc gggtcacgac gacctggtgg tctatgtctg ggtcgacgcc cccatcggct
     3961 acatctcctt taccgaggag tgggcacggg cgagggggct ggactggaaa cggtactggt
     4021 gcggaaagaa ccaggtcacc cacttcatcg ggagcgacat catctaccac cactgcatct
     4081 tctggcctgc ccttctcaag ggggcggggt attcccctcc cctcgccgtg gtcgcgagcg
     4141 ggatggtgaa gatcgatgac cagaagttct ccaagtcgag gggatacgtg gtctggacag
     4201 gagaggatta cctcgacaaa ggccttcctc ccgattatct ccggtactac ctcctctcgt
     4261 atacgaacca cacgaaggag ctgaacttct cctggaaggt cttttccgag cgggtgaaca
     4321 acgagctcgt ctccaccctc gggaacttca tctaccggag cctcttcttt gcccacaagt
     4381 tctttggccg ggtgcctgag gggccggtcg ggtccggaac ctcggaggcc atcggccgct
     4441 gcgtggagga catggacaag gcggtgcggg agttcgagtt caaaggggca actgacgccc
     4501 tcatggccct tgccgcctac ggaaacacct acatccagaa ccaggccccc tggaagctcg
     4561 tgaagacgga ccaggcggct gccgagaggg tggtgaagga ttgcctccag atcgcccatg
     4621 ccctcgccct cctgatggag cccatccttc cagatgcagc cggccgggta tggaggatgc
     4681 tcgggaacac ggacgcggtg gagacccacc ctgtcctgga tgcgaccagg cagataccct
     4741 cagttgctct tcccgagccc gcccccctct tcgagaagct cgaggagggt cggatccggg
     4801 agctggagaa gatcctgaag atgagaacgg agaaggcgaa gacggcagtg aaggagaagg
     4861 atctcatttc catcgaggag ttctcccgga tggacatcag ggtcgggaag atcctctcgg
     4921 cggaaccggt gaagggctcc aaaaagctcc tgaggttatc ggtggatatc ggcgaggaga
     4981 aatcccgcct ggttgtggcg ggcatggccg ggatccaccg gcccgaggag atggtggggc
     5041 tctcggtcat catggtcacg aacatgaaac cggcgaagat cttcggcatc gagagccagg
     5101 ggatggtcct tgcggcgggt gaggaggcga gcctcctcgt acccctgaag gaggtcccgc
     5161 cggggacgaa gatccggtga gaggctgccg ggacagggcg aaatcgtgag gtaccgcggg
     5221 catggtcgag acggacggat tcctgtttga atcgtgggct cccggcaagg agaccctgaa
     5281 ggctttcagt gacgggatat tcaccgtagc cgtcaccctc ctcgtcctcg aaatccggct
     5341 tcccgatata tcctcggggg agattgatgc ccgtttcctg gagagcctcg ctgccatcgc
     5401 gcccaagatg ctcggattca tcctgagttt ttttatcatc gcgatgtact ggctcctcta
     5461 tcaccgcctc tttcggtata tccgggagac cgacaggtcc ctggtcgggt tgaacatcct
     5521 gttcctcttc ttcatcgtcc tcatgccctt ccccacgtac ctcctgggcc tgtacgggga
     5581 ccaccggtcg atcgtcatgt tctacgcggg gatcgttgcc gtcacgagtg ccattcttgc
     5641 cttcatgtgg cgatatgcct cggtggaaca ccggctcgtc ggacccgacc ttgacgaccg
     5701 cgtgatccgg ctcatgtgga tgaggaccct tattcctgtc ggggtctttt cgctctccat
     5761 cctcgtggcc atcgcgagcc cgttcgtcgc aatggtcatg tgggtactga acttctttat
     5821 cattctgggg gtaacccggt tctatcgtcc cgataattcg aaacagcctg agacgaggtg
     5881 aaccggagga ttcaggtact tcccggactt ctgcccgtct cgaggagcct cttcagcccc
     5941 tcgagcacca ccttccagtt cccctcggca tgggccctcc cttcctcggt gggattgttg
     6001 tcctgggtga ccgtgaggag cgtaccaccg cctttttccg gcgtgagctc gtacctgacc
     6061 agcctgtagt tcccggggat atcgggaagc ccggacatgc tgctccagta ggtggtctcg
     6121 agcattttcc ctggctctgc tctcaggacc acccccttgt cctcgtaggc ctttccctcc
     6181 caggtacccc ggtacctgat ctggctcccg gccttccagt cggaagtggc ctccgtgccg
     6241 aacaggtact gcctgatcag gtcaggatcg gtgagggcct tccacacctc tgcaggagga
     6301 gccctgatgg agaccgattg ccgggcaacg agtcctttca tggccgggat gtgggtctcg
     6361 tcctgggaat cttcagcatg atgtccctcg aggaaaaccg gagatggctg ctgtcgcaga
     6421 agggcttgtt gaaggaatgg ccgcaacggc agagggtgac ccggttccgg atctcgtgca
     6481 cccagccgtc atgggagatg accgggatgc cgccccttac ccagaccggg ccgctcaggc
     6541 cttcctgggg gtactccacg aggccgatgg agtgctcgaa gtgcggctcg atgggaattc
     6601 tcgtcccctt ctcccagacc acgagacgac cggatggaca gtttcccgct tcctggatcg
     6661 cgatctcccg ggcctccctg ttccccgact cctgagtcag tttccagatc ccccccgctc
     6721 tctcgcagaa tttggccacg gaacagagga tctcggcatc ggtgagcacc aggttaggtc
     6781 ctccatactc cgtggcctgt ttgaggtagg gttcccggct cgcatgctcg gtcccgatga
     6841 agtggatctc cttgtggtac tggtcgcaga aaggcagcgt cctcgaccgg ccacatctgc
     6901 agagaatgta cgggttttcg acctcgaatt tcttctcctc cttccagacc gatgaatatc
     6961 cggcctcgtc cagggtgatc cgctgcaccg agagcgggat cccgccgaat acctggtacg
     7021 gaccgttctt gcagacgtgg atccggtgct caggtaatcc ggtgttcttc tgcccttttc
     7081 ctggagtttc cttccccgtt tctccttccg aatcactcat tgccggtctc tccctcacgg
     7141 tgttctctcc cggaaacgat ctgttctgcg ggtatgcatc ccgtcattac cagtctttat
     7201 taatttttcg tggaaaaaca acagatggga aacaaagcat tcttttcccc cggctgattg
     7261 atgtcagcct cggaaatgaa aggagcgggc ctggggggat ttgaaccccc gacatccggg
     7321 ttagaagccc ggcgctctgt ccaggctaag ccacaagccc ctggggagat agtgcgccct
     7381 catgggtatt gaaggctcgg aaaaaagggt tccccagggg atgcactagt gggcgtggta
     7441 gcaggtggtc tttaccacgt cgacgaggac cggccggccc atgccccggc tctcgatgtg
     7501 cttcaccacg gaggtgccgc agtattttac ctgcatccgg gcggccatcc gctcccgctc
     7561 ctcgaagaac atggggttcg tgaactcgat gatccgcttc atggagatgg cgacggagtt
     7621 cacctccacc atgatcccgg agtccccggg ccccaggtcg gcacagtcga ggctcgtaat
     7681 gaagacatgg tcgcccgcct tcacgccact gagggtgatg atgttgtggg ccgaggtgag
     7741 ctccacggtc ctcggcctcc cctgcttgag gtccgcgagg atggcagggg agatcccggt
     7801 tagcgctgca aaggacatgg ccctcacccg tacatgggga gctgctcttt cttctgccct
     7861 gcctcggtcg tctggacctc ctcggcgatc cggtgcatgg cggcctcgat ctcctcggcc
     7921 tgctccatga gcgggccgac atcgagcttg aggtcgtaca tcttgttcaa cacctggatg
     7981 gtcgcggccg aggaccgtgg gtccgggttg ttcaccgtct cgccgagaag cccgaggccc
     8041 cggatatccc ggatcttgca ctcggtgagg atggagccgg cgatacccga gatggagccg
     8101 atggggagga tcatcgtctg gtcctggatc cgctggaggg catcggggtt cgtcgcgacc
     8161 ccgaagaccc tcttctcggg ctcgttcgtg acgatcccgg cgatggtgag gacctccttc
     8221 ggcttgaacg gggcgaacca gtccagaagg ccgcatgcca cctcgtagca gatcatgggg
     8281 tggatgggga tatcccccac caccacggcg atgtcgttct tctcgtagaa ccggaccggg
     8341 acattgatga cccccttcgt catcatggcg aggggcggga agtacttgga ggtcaccgag
     8401 ccgatctggg ggaactcgag gttgtccacc aggtactgca gcgcgatgga acctacgagg
     8461 ccgctcccgg ggaacccgat gagcacggtc tttgtggtat cagcgagggg cctggatacg
     8521 attcgaatct cttccacagt gcatcacagc accttaacat agtggaagcc tcttaaaaaa
     8581 tattatgcag gagttccagg taaagcgggg ccatacgaag gatctcgcgg gggaggtgga
     8641 gaaggccttt accgactgct tcggggtgcg gccggagaag aatggggaaa cgtatgccat
     8701 ctcctacggg gctctctcga ggatggaggt gcagatcggc ggcggcggga agacgttgac
     8761 cgtgaacacc gtctcgcgga aggatgccgg ggaggcggcc atcctcgaca cgaaccggag
     8821 gttccggacc ttcctcgacc gggtgacggg gttcagctcc aaggaacggg tgaagcgggc
     8881 gaagaaggca gtagaagaat cgggttaatc taagtctcta tcgaatctgg aaggaaaaat
     8941 attatttttg atcat
//
LOCUS       DATGDI010000240         1609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_404626, whole genome shotgun sequence.
ACCESSION   DATGDI010000240 DATGDI010000000
VERSION     DATGDI010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404626"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VKO45_09060"
     CDS             complement(<1..467)
                     /locus_tag="VKO45_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06057.1"
                     /translation="MADDLFQHLPDPVWGAPLVSSPSQINKESLFPSADPALTVVAAQ
                     DSLRECMDDGSKCPCCGRPVQWSPIPLGAGKAVTLIRMMRYHDLHKVDWIHMLRDVNN
                     FGLPSRDYGSLEYWGFVEPKGVAKEDGNPDSGFWRLTQKGRAFAMGLSKCPFRA"
     gene            complement(480..710)
                     /locus_tag="VKO45_09065"
     CDS             complement(480..710)
                     /locus_tag="VKO45_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06058.1"
                     /translation="MRKTVLIVSRSGDVKMEVTAEFQIDTSAASGYDKENANKMVEER
                     VDTLLREVSDMTGVPIQNVKLRSIVIRQKRKR"
     gene            complement(723..956)
                     /locus_tag="VKO45_09070"
     CDS             complement(723..956)
                     /locus_tag="VKO45_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06059.1"
                     /translation="MAIIQSGNDPDCKHEKTTPEERSLLGCVPVYVSNCLACGEYLMR
                     FPTEDEVPKATMEKAFDATQQALRVLSQDPKAN"
     gene            complement(959..1168)
                     /locus_tag="VKO45_09075"
     CDS             complement(959..1168)
                     /locus_tag="VKO45_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06060.1"
                     /translation="MFICEPCCRERYKVLEDILVRAPAYVGTCKVCKKDERMCLDLNP
                     GCMIPKPYEPEPEQPAKKKAGKGKA"
     gene            complement(1169..1459)
                     /locus_tag="VKO45_09080"
     CDS             complement(1169..1459)
                     /locus_tag="VKO45_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06061.1"
                     /translation="MDSDCPCNCHTRPGSKYGCTFCAEHHAQPAETMEQIAHNAVREA
                     KQKLGPGGVVIVMLGDFMANNYGVWFSGPPLIVRGLKDAGNGLLDEQLNKKT"
BASE COUNT          242 a          545 c          473 g          349 t
ORIGIN      
        1 gcccggaagg ggcacttgga cagccccatg gcgaacgccc gtcccttctg ggtgagccgc
       61 cagaatccgc tgtccggatt cccgtcctcc ttggcgacgc ccttcggctc cacgaacccc
      121 cagtattcga ggcttccgta gtcccggctg gggagaccga agttgttcac gtcccgaagc
      181 atgtgaatcc agtccacctt gtggaggtcg tggtagcgca tcatccggat caaggtgacc
      241 gccttccccg cccccagcgg gatcggggac cactgcacgg gacggccgca gcacgggcac
      301 ttgctcccgt cgtccatgca ctcccggagg gagtcctgcg cggcgacgac ggtgagggcg
      361 gggtcggccg acgggaacag cgactccttg ttgatctggg aggggctgct cacgagcgga
      421 gcgccccaga ccggatcggg aaggtgctgg aagagatcgt cggccatgtc cttcttctcc
      481 taccgcttcc gcttctgccg gatgacaatg gaccgcaact tcacgttctg gatcgggact
      541 ccggtcatgt cggacacctc ccgcaggagc gtgtccacgc gctcctccac catcttgttg
      601 gcgttctcct tgtcgtaccc gctggccgcg ctcgtgtcga tctggaactc ggccgtgacc
      661 tccatcttca cgtccccgct cctcgaaacg atcagcacgg tcttacgcat ggcggtctcc
      721 tttcagtttg ccttcgggtc ttgggaaagc accctcaatg cctgctgggt cgcgtcgaac
      781 gccttctcca tggtcgcctt cgggacctcg tcctcggtcg gaaaccgcat gaggtactcc
      841 ccgcacgcga ggcagttcga gacgtagacg ggaacgcagc cgaggaggga ccgctcttcg
      901 ggcgtggtct tttcgtgctt gcagtccgga tcgttcccgc tctggatgat ggccacctct
      961 acgcctttcc cttgcccgct ttcttctttg cgggctgttc aggctccggt tcgtacggct
     1021 tcgggatcat gcagccggga ttcaggtcga ggcacatccg ctcgtccttc ttgcaaacct
     1081 tgcaggtccc cacgtacgcc ggggcccgga cgagaatgtc ctccagtacc ttgtaccgct
     1141 cccgacagca gggttcgcag atgaacattc aggtcttctt gttcagttgt tcatcgagca
     1201 atccgttccc ggcgtccttg agtccgcgca caatgagagg cggtccgctg aaccacactc
     1261 cgtagttgtt cgccatgaag tcgcccagca tgacgatcac cactccaccg gggcccaact
     1321 tctgcttggc ttcccggacc gcattgtggg caatctgctc catggtctct gcgggttgag
     1381 cgtggtgctc cgcacagaac gtgcagccgt acttgcttcc cggccgcgtg tggcagttgc
     1441 acgggcagtc gctgtccatg tcctatcccc tcacctgtcc agccttcctc cgggcccgcc
     1501 cccgtatgct ctcgatgaaa aggcggtcct ccgacctcac ggagcgccct tttccccctc
     1561 tggaaccgtg gtttttcggg gtagaagagg ggtcgcgagg ggctcctcg
//
LOCUS       DATGDI010000241         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_230181, whole genome shotgun sequence.
ACCESSION   DATGDI010000241 DATGDI010000000
VERSION     DATGDI010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230181"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1016)
                     /gene="msrA"
                     /locus_tag="VKO45_09085"
     CDS             complement(255..1016)
                     /gene="msrA"
                     /locus_tag="VKO45_09085"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HMA06062.1"
                     /translation="MGKAKLKKATFAGGCFWCMQPPFRMIDGVVDVVSGYAGGTKENP
                     TYDEVSSGTTGHLEAVQVTYDPARVSYDTLLDTFWRQIDPTDPGGQFADRGSQYRTAI
                     FYHDEEQKRLAEASKKRLEASGKFPAAVATEIRPYTNFYPAEEYHQDYDRKNPERYHQ
                     YKVLSGRERFIEKTWGKNRVVRVYSTPGCTGCRAVKEFLKARNVEFTEIDMAADEAAR
                     NFVMEKTGFLGSPVVQIGDVFIVGFDPKKMAKALG"
     gene            1360..1791
                     /locus_tag="VKO45_09090"
     CDS             1360..1791
                     /locus_tag="VKO45_09090"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMA06063.1"
                     /translation="MARIEESIEIRRPVEQVFSFTTDAKSWPKWQSIIMEAEQTSPGP
                     VTVGTMFRGTSRMMGRTMPWTAKATEFEANRRYGKNIDSGPVTIEQHNAYQSVEGGTR
                     FTILYDMKVGGFMKLMSPMITGSMRKELKKSLGNLKKVLEA"
BASE COUNT          450 a          585 c          626 g          401 t
ORIGIN      
        1 ccgtttccgg gccccgtcgg gtgaatccct gccgggcccc ggtccggtgt ccacgggaaa
       61 gaaacaggca cagtaccggc attcccagat gcccgggatc ggggtctctc ccgtgaggag
      121 ggaaccgcat ccgggacacc ggaagtcggg gcggcgtacc atagggagga aaggagaggc
      181 gggtttatca cggtgaggcc gggagggggt gaaagtggac ccgggccgga gaattcgtcc
      241 cgacccgatc ccccttaccc gagggccttt gccattttct tcgggtcaaa cccgacgatg
      301 aacacgtccc ctatctgcac gacgggcgaa ccgaggaacc cggtcttttc catcacgaag
      361 ttcctcgccg cctcgtccgc cgccatgtcg atctcggtga actccacgtt tcgtgccttc
      421 aggaactcct tgaccgcgcg gcacccggtg catcccggcg tggagtacac ccggacgacc
      481 cggttcttcc cccaggtctt ctcgatgaat cgctcgcgac cggagaggac cttgtactgg
      541 tggtaccgct cggggttctt gcggtcatag tcctggtggt attcctccgc cgggtagaag
      601 tttgtgtacg gccggatctc ggtggcaacg gccgcgggaa acttcccgga tgcctcgagc
      661 ctctttttcg aggcctcggc gagcctcttc tgctcctcgt cgtggtagaa gatggcggtc
      721 ctgtactggg atccccggtc cgcgaactgc cctccggggt cggtgggatc gatctgcctc
      781 cagaaggtgt cgagaagcgt gtcgtaggag acccgggcgg gatcgtaggt cacctggacc
      841 gcctcgaggt gccccgtcgt ccccgacgag acctcgtcgt aggtcgggtt ctccttcgtt
      901 ccgccggcat agcccgagac cacatcgacc acaccgtcta tcatcctgaa cgggggctgc
      961 atgcaccaga agcatcctcc ggcaaaggtg gccttcttca gtttcgcctt tcccaagccg
     1021 gtaccggttc cagaactccc tttctccagg cgtatcaccc agccgtatca gggaagagca
     1081 gcaacagggg ataaaggttt gtactggatc tatcgccata tatcgaacgg gataccctgg
     1141 tgaagtccgg ccttccgggc cagctcaacc gcctggggat ttacctccgg cccaatgata
     1201 ctgcccatgg cctctccctc ccaagccacc cggcggtatc tttatggatt cgcgtgcaaa
     1261 tgtccaccgt gtcctgagcc gggtggggcc ccttcagttc aggagcggca caccggcccg
     1321 gtttcatttt tatacttttc aggacaggga gggagtcgga tggcgagaat cgaggagagc
     1381 attgagatca ggcgtccggt cgagcaggtt ttcagtttca cgaccgatgc gaagagctgg
     1441 ccgaagtggc agtcgatcat catggaggcg gaacagacgt ctccgggacc cgtcaccgtc
     1501 gggacaatgt tccggggaac gagccggatg atgggccgca ccatgccgtg gaccgcgaag
     1561 gcgacggagt tcgaggccaa ccggagatat ggcaagaata tcgactcggg acccgtgacc
     1621 atcgaacagc acaatgccta ccagtctgtg gagggcggca cgaggtttac catcctctac
     1681 gatatgaagg tggggggatt catgaagctc atgtccccca tgataacggg ttcgatgcgc
     1741 aaagagctga agaaaagcct cggcaacctg aagaaggtac tggaggcgta atccgctgta
     1801 ggcaaaccac cggtatgcaa ggaggcaagt ttcccccgat ttagataacg cgaaaacaag
     1861 tgagcggtcg tgattcagtg attcacgaac agggcaaacc ataactatta gggggggtcc
     1921 aaaaattaca ataatgaaag aaataactga aaaagatatt attgatgcac taaatcgggg
     1981 gattgctgta tatacggagg cggcagaaaa ggagggtaga gaaaatactc catgggaaaa
     2041 agagcaaata aaagaagcat ac
//
LOCUS       DATGDI010000242         6355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_200849, whole genome shotgun sequence.
ACCESSION   DATGDI010000242 DATGDI010000000
VERSION     DATGDI010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200849"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..654)
                     /locus_tag="VKO45_09095"
     CDS             complement(262..654)
                     /locus_tag="VKO45_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HMA06064.1"
                     /translation="MSVARFWRKIPQRYNLVGTKCEHCGRHYFPPRSFCPDCRRGGKI
                     VEYRFPGTGKVVTHTVIRSASEQFENTTPYVLAIIQLDEGPRLTAQVVAKPEEVLVGT
                     RVRSVFRRIMADGESGVIHYGTKFVPVK"
     gene            complement(656..1819)
                     /locus_tag="VKO45_09100"
     CDS             complement(656..1819)
                     /locus_tag="VKO45_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HMA06065.1"
                     /translation="MRDVAVIGIGCTTFGEKWESSFRDLFVEAGSLALADAELSGENI
                     DAMYVGNMSAGRFIEQEHIGALIADYAGLSTRHIPSTRVEAACSSGGLAFRQAVIAVA
                     SGMEDIVVAAGVEKMTDVGTGASVDTLAGAADREWEGFMGATFPGLYAMIATDYMHRY
                     PLTREQLGMVAVKNHRNGAKNPIAQFRQEITLETVLSSSLVADPLRLFDCSPITDGAA
                     AVILAPLERAREFTDTPVKVLASTQASDTIALHDRRDISTLDATVFASRRAFALARIP
                     MSKIDFLEVHDCFTIAEICAIEDLGFCKKGEAGRLTEDGVTALDGDLPVNTSGGLKAC
                     GHPVGATGIKQVCEVVQQLRGEAGARQVDGEIGLTHNVGGTGATVVVHILGVT"
     gene            complement(1816..2871)
                     /locus_tag="VKO45_09105"
     CDS             complement(1816..2871)
                     /locus_tag="VKO45_09105"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HMA06066.1"
                     /translation="MVGIVTYGVYIPRFRIRVEEIARVWGANADEITGGLGVFEKSVP
                     DLDEDTATIAVEAARAAMARARVDPAEIGAVYVGSESHPYAVKPTACTVGEAIRATPE
                     MTAADYEFACKAGTAGIQTCMGLVKSGMIAYGMAIGSDVAQGAPGDALEYTAAAGGAA
                     FLIGNDRPIAEIHHTCSFTTDTPDFWRREGQDYPRHGGRFTGDPGYFRHVQGAARMLL
                     SRMGTSPSDYDYAVFHQPNAKFPRKVAQLLGFSAQQVQPGLVVPLLGNTYSGSSMIGL
                     AATLDQAKPGDRIFVTSFGSGAGSDAFDIEVTDAIADPAVFDRGQAPTVDALLADPVY
                     LDYAQYARHKGKIVMQQ"
     gene            complement(2865..3575)
                     /locus_tag="VKO45_09110"
     CDS             complement(2865..3575)
                     /locus_tag="VKO45_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMA06067.1"
                     /translation="MTSELRQHLAEKMAGEITLSDSPGKALKKWRMAFEIPAAVLADE
                     MGMSPSVISDYESGRRRSPGTAVVGRIVDTILAIDQESGGKFIGKFGRILENRFDSDI
                     IFDIHEYASPVPLEAFARAIGATIISGPMDREIYGYTVINSLNAILGLSSSEFGRLYG
                     WSTERALVFTEVSNGKSPMVAIRVTPFKPRCVVLQGITPEEIHPLVPKLAERDRITLM
                     STPLKVEEIIEALRSEKW"
     gene            3710..4378
                     /locus_tag="VKO45_09115"
     CDS             3710..4378
                     /locus_tag="VKO45_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HMA06068.1"
                     /translation="MLEPGDLQCLKAIALMGGCRGPVWISSQSFGESLSMSPQTASRR
                     LKALEGQRLIDRSLRPDGQYVTVTRDGERALRREFADYCQIFGEGGGPYSLTGSVISG
                     LGEGRYYMSLPAYQAQFREILGKDPFPGTLNLRLSSASRDVKKKVDDLPWTRIQGFVQ
                     DGRTFGDARSIPCRIGDIPCAIVIPGRTHYPDDVIEVIAPVELRAALGLSDGDLVTVE
                     VAHA"
     gene            4371..5051
                     /gene="ribB"
                     /locus_tag="VKO45_09120"
     CDS             4371..5051
                     /gene="ribB"
                     /locus_tag="VKO45_09120"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HMA06069.1"
                     /translation="MLEEAIAAFRDGKFILLYDFDNRERETDFAIRADAVTRHHVMWM
                     RKDGGGLICTAVHPDAAKRLGLPFARDLMASYQGVERLGEIPYDPKNPSSFSIWVNHR
                     NTFTGITDRDRSLTINAIADEVKAAMKGRNGTEFHRIFRTPGHVAILRAAEGLLDQRC
                     GQTELSIALADFARVTPAVTICEMLDDETGYALPKEDAIRYAGRHGLPFVEGCEVLER
                     WEREKVSR"
     gene            complement(5060..5233)
                     /locus_tag="VKO45_09125"
     CDS             complement(5060..5233)
                     /locus_tag="VKO45_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06070.1"
                     /translation="MVMGMKEAELKELVSRISSADLKGEVKKRGIKMGRCPTKMDFAK
                     KLPEEVLQRLAEK"
     gene            5322..5594
                     /locus_tag="VKO45_09130"
     CDS             5322..5594
                     /locus_tag="VKO45_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06071.1"
                     /translation="MIRLICRQCKGTGKIPNIAYEMCRTMQSHDAKRYFYMNQEHDAS
                     EENNKSDGCAIHEKSVSCPQCDGNGVLEFDEEEWELSVMPEEDEEG"
     gene            complement(5881..>6355)
                     /locus_tag="VKO45_09135"
     CDS             complement(5881..>6355)
                     /locus_tag="VKO45_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HMA06072.1"
                     /translation="GLFCMENFPYKSMQCLVEDHAGQSLTSVKKIEIGKGKYWVYTER
                     GNVVTLPLKVTHKYEQPGCHVCLDYVSNLGDISTGSVGSPDGWNTVFVRTKVGTDLWS
                     KITASGMVETKPIESVQPGLAMVTKLAKEKIDKNRKTLEERKTFGINKALRDYTA"
BASE COUNT         1104 a         2088 c         2016 g         1147 t
ORIGIN      
        1 ggcgtcccca agggcgatga ggcgttgatc ccctgcaccg ttacatcggg agttcccgga
       61 aacggagggg ggtgcccgtg cggcgggggg cgtagcactc gcaacttcgt cgctcaaggt
      121 cggtttctta cgaaacctcc tggtcgcacc ccgcctgatg gcggtttgct ccaggtcggg
      181 ttctgatgaa ccctccctcc cccgagagca cccaaagaaa aagacgacga atgtaatccc
      241 tttcgtggcg tcgctaaaag attatttcac cggtacgaac ttcgtaccat agtggatcac
      301 gccggattcg ccgtcggcca tgatccggcg gaagaccgac ctgacccggg tccccaccag
      361 aacctcctcg ggtttcgcca ccacctgggc ggtgaggcgc gggccctcgt cgagctggat
      421 gatggcgagg acgtacgggg tggtgttctc gaactgctcc gaggccgaac ggatcacggt
      481 gtgggtgacc accttcccgg ttcccgggaa ccggtattcg acgatcttcc ctcccctccg
      541 gcagtccggg cagaaagacc tcggcgggaa gtagtgccgc ccgcagtgct cgcacttcgt
      601 cccgaccagg ttgtagcgct gggggatttt cctccagaac cgggcgacgg acatctcagg
      661 tcacccccag gatatggacg accacggtcg cgccggtccc cccgacgttg tgggtgagcc
      721 cgatctcccc gtccacctgc cgggctcccg cctctccccg gagctgctgg accacctcgc
      781 agacctgctt tataccggtc gcaccgacgg ggtgcccgca tgccttcagg ccgccgctcg
      841 tgttcaccgg gaggtccccg tcaagggcgg taactccatc ctcggtgagc ctccccgcct
      901 cccccttttt acagaagccg aggtcctcga tggcacagat ctcggcgatg gtgaagcagt
      961 cgtgcacctc gaggaaatcg atcttcgaca tcgggatacg ggccagggca aaggcacgcc
     1021 tgctggcgaa caccgtcgcg tccagggtgg agatgtcccg gcggtcgtgg agggcgatgg
     1081 tatcgctcgc ctgggtgctg gcgaggacct tcaccggcgt gtcggtgaac tccctcgcgc
     1141 gctcgagggg ggcgaggatg accgccgccg ccccgtcggt gataggtgag cagtcgaaga
     1201 gccggagagg gtctgccacg agggaggagg agaggaccgt ctcgagggtg atctcctgcc
     1261 ggaactgggc gatggggttc ttcgccccgt tccggtggtt cttcaccgcg accatgccga
     1321 gttgctcgcg ggtgagcggg tagcggtgca tgtagtcggt ggcgatcatg gcatagaggc
     1381 cggggaaggt cgcccccatg aagccctccc actcccggtc cgcggctccg gcgagagtgt
     1441 cgacgctcgc gccggtgccg acgtcggtca tcttctcgac acccgccgcg acaacgatat
     1501 cctccatccc gctcgcgacg gcgatgaccg cctgccggaa ggcgaggccc cccgacgagc
     1561 aggcggcctc gacccgggtc gaggggatgt gccgggtgga gaggcccgcg tagtcggcga
     1621 tgagggcccc gatgtgctcc tgctcgatga accggccggc gctcatgttc cccacgtaca
     1681 tcgcatcgat gttctcgccg gagagctccg catccgcgag ggcgagggaa cccgcctcga
     1741 caaagaggtc ccggaaggac gactcccact tctcgccaaa ggtggtgcac ccgatcccga
     1801 tcactgccac gtctctcatt gctgcatcac gatcttcccc ttatgtcgcg cgtactgggc
     1861 atagtcaagg tacaccggat ccgcgaggag ggcatcgacg gtcggtgcct gtccccggtc
     1921 gaaaacggcc ggatcggcga tggcatccgt cacctcgatg tcgaaggcat cgctccccgc
     1981 accggatccg aaggaggtga cgaagatccg gtcccctggc tttgcctggt ccagggtcgc
     2041 cgcaagcccg atcatggagg atccggagta ggtgttcccc aggagcggga cgacgaggcc
     2101 gggctgcacc tgctgtgcgg agaacccgag gagctgggca accttcctcg ggaacttggc
     2161 gttcggctga tgaaagaccg catagtcgta gtcggatgga gaggtgccca tccgcgaaag
     2221 aagcatccgg gccgcgccct gcacgtgccg gaagtagccc gggtcgccgg tgaaccttcc
     2281 cccgtgtctc gggtagtcct gcccttcccg ccgccagaag tccggggtat ccgtggtgaa
     2341 ggagcaggtg tggtggatct ccgcgatggg ccggtcgttc ccgatgagga aggccgcacc
     2401 ccccgccgcc gccgtgtatt ccagggcatc ccccggcgcc ccctgggcga catcggagcc
     2461 gatggccatc ccgtacgcga tcatcccgct cttcacgagg cccatgcagg tctggatgcc
     2521 ggccgttccc gccttgcagg cgaactcgta gtctgcggcg gtcatctcgg gggtcgcccg
     2581 gatggcctcc ccgacggtgc aggccgtggg cttgacggcg tagggatggg actccgaccc
     2641 cacgtacacc gcgccgattt cggcgggatc gacccgggcc cgcgccatgg ccgcccgcgc
     2701 cgcctcgacg gcgatggtcg cggtgtcctc gtcgagatcc gggaccgact tctcgaagac
     2761 cccgagcccc cccgtgatct cgtcggcgtt cgccccccag acccgggcga tctcctccac
     2821 ccggatccgg aacctcggga tatagacccc gtacgtgacg atgcctacca tttctcgctc
     2881 ctcagggcct cgatgatctc ctcgaccttc aggggggtgg acatgagggt gatccggtcc
     2941 cgctccgcga gtttcgggac gaggggatgg atctcctcgg gggtgatccc ctggaggacc
     3001 acgcaccggg gcttgaaggg ggtgacccgg atggcgacca tcggggattt cccgttcgac
     3061 acctcggtga agacgagggc ccgctccgtg gaccacccgt acagcctgcc gaactcgctc
     3121 gaggagagtc cgaggatggc gttcaggctg ttgatgaccg tgtacccgta gatctcccgg
     3181 tccatgggtc cgctgatgat ggtggccccg atggcccggg caaaggcctc gaggggaacg
     3241 ggcgaggcgt actcgtggat gtcgaagatg atgtccgaat cgaaccggtt ctccaggatc
     3301 ctcccgaact tcccgatgaa ctttcccccg ctctcctggt cgatggcaag gatggtatcc
     3361 acgatcctcc cgaccaccgc cgtccccggg ctccgcctcc tcccgctctc gtagtcgctg
     3421 atcaccgacg gggacatgcc catctcgtcg gcgaggaccg cagccgggat ctcgaaggcc
     3481 atcctccact tcttgagggc cttccccggc gagtcggaga gggtgatctc ccctgccatc
     3541 ttctcggcaa ggtgctggcg gagctcggat gtcatgcctc catggttgcc cgggaggggt
     3601 tatataatta acgaacctga cttcgacatt tcacgaactc cccccgagct cccctgcccg
     3661 gaacgggggt cagaatccat atataggatg catcccaatt ctgataagca tgctcgaacc
     3721 cggcgatctc cagtgcctga aggccatcgc cctcatgggc ggctgcaggg ggcctgtctg
     3781 gatctcctcg cagtccttcg gcgagtccct ctccatgagc ccccagaccg cctcccggcg
     3841 gctcaaggcc ctcgagggcc agcggctcat cgaccggtcc ctccgccccg acgggcagta
     3901 cgtgaccgtc acccgcgacg gcgagagggc actccggagg gagtttgccg actactgcca
     3961 gatcttcggg gagggtggcg gcccctacag cctgaccggc tccgtcatca gcggtctcgg
     4021 cgaggggcgt tactacatga gcctgcccgc gtaccaggcg cagttccggg aaatcctcgg
     4081 gaaggacccc tttccgggca ccctgaacct ccggctctcc tcggcctccc gggacgtaaa
     4141 gaagaaggtg gatgacctcc cctggacacg gatccagggg ttcgtccagg acggccggac
     4201 ctttggtgat gcccggagca ttccctgccg gatcggggat atcccctgcg ccatcgtcat
     4261 ccccggccgg acccactacc ccgatgacgt catcgaggtg atcgcacccg tggagctcag
     4321 ggccgccctc ggcctctcgg atggcgatct cgtgaccgtg gaggtcgccc atgcttgagg
     4381 aggccatcgc ggccttccgg gacgggaagt tcatcctcct ctacgacttc gacaaccgcg
     4441 agagggagac cgacttcgcc atccgggccg acgcggtgac caggcaccac gtcatgtgga
     4501 tgaggaagga cggcgggggg ctcatctgca ccgccgtgca ccccgacgcg gcaaagaggc
     4561 tcggccttcc cttcgcccgg gacctcatgg cctcgtacca gggcgtcgag agactgggcg
     4621 agatccccta cgacccgaaa aatccctcct ccttctccat ctgggtgaac caccggaaca
     4681 ccttcacggg catcaccgac cgggaccggt ccctcaccat caacgccatc gccgacgagg
     4741 tgaaggccgc gatgaagggg cggaacggca cggagttcca ccgcatcttc cggacccccg
     4801 gccatgtcgc catcctccgg gcggccgagg ggctgctgga ccagcggtgc ggccagaccg
     4861 agctctccat cgccctcgct gacttcgccc gggtcacccc ggcggtgacc atctgcgaga
     4921 tgctcgacga cgagaccggg tatgccctgc caaaggagga tgccatccgg tatgccggca
     4981 ggcacggcct tcccttcgtc gagggatgcg aggtcctcga gcggtgggag cgggagaagg
     5041 tctcccgata aaaagccggc tacttttccg cgagcctctg gagcacttcc tcgggaagct
     5101 tcttcgcgaa gtccatcttc gtcgggcagc gtcccatctt gatgcccctt ttcttcacct
     5161 ctcccttcag gtctgccgag ctgatccgac tcaccagctc cttgagctcc gcctccttca
     5221 tgcccatcac catgggataa taccccgttc cttccttaaa ctttacccgg catgaaatac
     5281 gagagattaa gggatggaat gcctaccctc agtgaaagga catgatacgt ctcatctgca
     5341 ggcagtgtaa ggggacggga aagatcccga acatcgccta cgagatgtgc cgaaccatgc
     5401 agtcccatga tgcgaagcgc tacttctaca tgaaccagga gcatgacgct tccgaggaga
     5461 acaataagtc cgacgggtgc gccatccatg aaaagtccgt gtcctgcccg cagtgcgacg
     5521 ggaacggggt cctcgagttc gacgaggagg agtgggagct ctcggtcatg cccgaggagg
     5581 acgaggaggg gtgaagtcgg ccgatctggt cgatcccacc aagatatcct cgctacagaa
     5641 tgggtggagg agggggtggc gggtgggaga agccaccctt cgaggatgct tgatagaaat
     5701 cctcagctca gcaaactatc tcctgcacgg gaaatggatg gttgtccggc ggacgctctc
     5761 gggggcttcg cccccgccgc tgtggcaccc ccgatgcgat gggacgatga atgagggtca
     5821 gcaacagggc tgaactgcat ggaagtgctc tgagaatcga aggtaaaaaa aaggtgagat
     5881 tcaggcggtg tagtcccgca gagccttgtt gatgccgaag gtcttgcgct cctccagcgt
     5941 cttccggttc ttgtcgatct tttccttggc gagcttcgtg accatcgcaa ggccgggctg
     6001 gaccgactcg atgggcttcg tctcgaccat gccggacgcg gtgatcttgg accagaggtc
     6061 tgtgccgacc ttggtccgga caaagaccgt gttccagcca tcgggactcc cgaccgagcc
     6121 ggtcgagatg tctccgaggt tggagacata gtccaggcag acatggcaac cgggctgctc
     6181 gtacttgtgg gtcaccttca gggggagcgt gacgacgttt ccccgctcgg tgtagaccca
     6241 gtacttgccc ttcccgatct cgatcttctt cacggaagtc aggctctggc ccgcatggtc
     6301 ctcgaccagg cactgcatgg acttgtacgg gaagttttcc atgcagaaga ggccg
//
LOCUS       DATGDI010000243         2538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_259720, whole genome shotgun sequence.
ACCESSION   DATGDI010000243 DATGDI010000000
VERSION     DATGDI010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259720"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VKO45_09140"
     CDS             <1..1057
                     /locus_tag="VKO45_09140"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMA06073.1"
                     /translation="FSMSGETARYPRGSEAQESGILRPFLIIVFTLVVLLLNGYALSH
                     AGDSFFRQISPILPHLYYIPIILASVWYPRRGLLLIGLVVGVSLGIVLFGLVNGTFSP
                     VLLLYPAIYVWVVATISFFLIGQWRSLSRLIPARRVRGARGGDSGGAPLPGGSSLPAS
                     GEDVDSLLSTLGIQDDRVREAAIASLGRSLDPRALPPLIGLLGDESRGIREASVRALG
                     AFGPGAVEPLIGALGSGDWHIRMGGVIALRIIGDPRGVEPLIQALTDENRFVRREAAK
                     SLGRLGDGRATDPLITLLSDRDTGVRVRAAGALGKIADPRAIGPLTRALSDRNSELQQ
                     AAVEALSKIKNSRTSAD"
     gene            1159..1752
                     /locus_tag="VKO45_09145"
     CDS             1159..1752
                     /locus_tag="VKO45_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellin/type IV pilin N-terminal
                     domain-containing protein"
                     /protein_id="HMA06074.1"
                     /translation="MKRLLSREDAFTGLEAAIVLIAFVVVAAVFSYVVLGAGFFSTQK
                     SQEAVHTGVQQASSSFEILGDVYGVDTGAGKVQINYLNFTIGLAPGGMPIDMSKLTMV
                     YANQSVRNDINQSVPLIANTNPAVNQWAIARVINGNNNALLEGGEQMDIVVHLAESTG
                     SIAARDTFTLEIKPIIGPALSISRTAPGGIDPVNKLY"
     gene            1766..2362
                     /locus_tag="VKO45_09150"
     CDS             1766..2362
                     /locus_tag="VKO45_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellin/type IV pilin N-terminal
                     domain-containing protein"
                     /protein_id="HMA06075.1"
                     /translation="MKKFAKSEAFTGLEAAIVLIAFVVVAAVFSYVVLGAGFFTTQKA
                     QQVVYSGVQQASSNIIIIGEVYGDASGTLNPPQVHAVYFTVGLAAGGSSVDFNKTVFT
                     YQNRTILKTIDFASNILGNNGLQPADWQWMIKTRQNDDGDAALERNEQFTIQVNLSNT
                     QGLPPSEDFTLTIRPDIGAALPIHKSAPGGLTPMNVLY"
BASE COUNT          498 a          780 c          732 g          528 t
ORIGIN      
        1 tttctccatg tcaggggaga cggcccggta tccccggggg tccgaggcgc aggagagcgg
       61 gatcctgagg ccgtttctca tcatcgtctt taccttggtc gtcctccttc tcaacgggta
      121 cgccctgtcc catgcgggag actccttctt ccgccagata tcccctatcc tccctcacct
      181 ctattacatc cccatcatcc tcgcctcggt ctggtacccc aggcggggtc tcctcctcat
      241 cggccttgtc gtcggtgtct ccctggggat agtcctcttc ggcctcgtga atggcacctt
      301 cagcccggtc ctcctcctct atcccgccat ctacgtctgg gtggtggcga ccatctcgtt
      361 tttcctcatc gggcagtgga ggagcctctc ccggctcatc cccgcgcgaa gggttcgggg
      421 ggcccgggga ggagattcgg ggggagctcc ccttccgggt ggttcgtcat tgccggcatc
      481 gggggaggac gtggactccc tgctctcgac cctcgggatc caggacgacc gggtcaggga
      541 ggcggccatc gcatccctcg ggaggagcct ggacccgagg gccctccctc ccctcatcgg
      601 cctcctcgga gacgaaagcc ggggcatcag ggaggcatcg gtccgggccc tcggggcctt
      661 cgggcccggg gcggtggagc cgctcatcgg ggccctcggg agcggggact ggcacatccg
      721 catgggagga gtgatcgccc tccggatcat cggggatccc cggggagtgg aaccgctgat
      781 ccaggccctt accgacgaaa accggttcgt gaggagagag gccgcgaagt ccctgggcag
      841 gctgggggac ggaagggcca ccgatcccct gatcacgctc ctttcggaca gggacaccgg
      901 ggtcagggta cgggcggcgg gcgccctcgg gaagatcgcg gacccgaggg ccatcggtcc
      961 gctcacccgt gccctctctg accggaattc cgaactgcag caagcggcgg ttgaagccct
     1021 atccaaaata aaaaattcca gaacttcagc tgattaaaca ggatttaacg tggtttaaat
     1081 agaaaagtat ttatagagat aaaaggattt aacaatgagt atacctggag agattcggga
     1141 tactgacagg tgtagtctat gaaacgtcta ctgagtcgtg aagatgcctt caccggcctc
     1201 gaggcggcca tcgtgctcat cgccttcgtg gtcgtggctg cggtcttctc ctacgtggta
     1261 ctcggtgccg ggttcttctc cacgcagaag agccaggagg ccgttcacac gggcgtccag
     1321 caggcaagct cgagcttcga gatcctgggg gatgtctatg gggttgatac aggcgccggc
     1381 aaggtgcaga tcaactacct gaactttacc atcggtctcg caccgggcgg catgcccatt
     1441 gacatgagca agctaaccat ggtctatgcg aaccagagtg tccgcaatga catcaaccag
     1501 tcggttccgc tgatcgcgaa caccaatccc gcggtaaacc agtgggccat tgcccgtgtc
     1561 atcaatggga acaacaacgc cctcctcgag ggaggggaac agatggacat cgtcgttcac
     1621 ctcgccgaga gcactggctc catcgcggca cgcgacacgt tcacccttga gatcaagcct
     1681 atcatcgggc ctgcgctctc catatcgcgg accgcgcccg gcgggatcga cccggtgaac
     1741 aagctctact aggaggtgca tgaagatgaa gaaattcgct aagagtgagg ccttcactgg
     1801 cctcgaggcg gccatcgtcc tcatcgcctt cgtggtggtt gccgcagtgt tctcgtacgt
     1861 ggtgctcggt gccgggttct tcacgaccca gaaggcccag caggtcgtgt actcgggtgt
     1921 ccagcaggcc tcctccaaca tcatcatcat cggcgaggtg tacggggatg cttctggtac
     1981 cctcaatccg ccccaggtcc acgccgtcta ctttaccgtc gggctcgctg ccggcgggtc
     2041 atccgttgac ttcaacaaga cggtctttac ctaccagaac cggaccatcc tgaagaccat
     2101 cgacttcgcc tcgaatatcc tcgggaacaa cgggcttcag cccgccgact ggcagtggat
     2161 gatcaagacc cggcagaatg acgatgggga tgcagccctt gagaggaacg agcagttcac
     2221 catccaggtc aacctgagta acacccaggg gctcccgccc tctgaggact tcaccctcac
     2281 gatccggccg gacatcggtg cggctctccc gattcacaag tcagctccgg gtggtctcac
     2341 ccccatgaat gtgctctatt aattcccctt tttttccggt aatccgggat cccggttaga
     2401 atgaagggtg acagttcctt cagctccatg agcccaggat cggctccaat tctccatttt
     2461 ataacttaat atttggctat tttgtaaaca cgataccatt tatgtaaaat aacgaaacac
     2521 aaaatatatt tagtatga
//
LOCUS       DATGDI010000244         4975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_171936, whole genome shotgun sequence.
ACCESSION   DATGDI010000244 DATGDI010000000
VERSION     DATGDI010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171936"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..822
                     /locus_tag="VKO45_09155"
     CDS             253..822
                     /locus_tag="VKO45_09155"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HMA06076.1"
                     /translation="MGKRIVITGVPGVGKTTVVNGALEKLAQEGISYQSINFGTFMFE
                     VAANQKMVSDRDGMRRLPGAVQKDIQRKAAQAIAKIEGNIIIDTHCTVKTPKGFLAGL
                     PEWVLRELMPDMVILVETDEDQILSRRLTDTTRTRDPDSSKEIALHQEYNRAMAAAYG
                     MVSGCTVKIVKNQDFLLDRAIGDMADALR"
     gene            828..1448
                     /locus_tag="VKO45_09160"
     CDS             828..1448
                     /locus_tag="VKO45_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF106 domain-containing protein"
                     /protein_id="HMA06077.1"
                     /translation="MASKSSGSMTSLALAMGLIFLSTIPAVRQFVGSAMDFILGPLLA
                     EPFPGLPFYVVILILSALTGLYSSLLQKYTIDYDRVLSVQEGMKEFQKEYRDAQLSKD
                     EKRIKKLESKRDKMMKEQLEVSQEQFKPMAYILLVSIPIFLWLIYKMPTLSPESSAGI
                     ITFPYFGSLAIWNGAIWIVPAWILWYMICSISISQVIRKSMNIGGI"
     gene            1448..1975
                     /locus_tag="VKO45_09165"
     CDS             1448..1975
                     /locus_tag="VKO45_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMA06078.1"
                     /translation="MKITVSGPPGSGTTTLARFIAEKHKFELISAGELFRDLATEKGL
                     SVAEFGELAETDITIDALIDVRQKETAKTRDNIVVEGRLSGWMVEEADLRIWLNAPIA
                     CRAKRIATRDGMDEYTARELTLQREESERRRYRNYYKIEIDDLSPYHLAMNSELWSGE
                     ALADIVDLAIGHLKK"
     gene            complement(2006..3850)
                     /locus_tag="VKO45_09170"
     CDS             complement(2006..3850)
                     /locus_tag="VKO45_09170"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMA06079.1"
                     /translation="MTMKQDRHQWTFWDFFLIGLVLLSVFIANVFIYYLYQGTVFVLT
                     NFFYIPVIFAAFQYPKRGIFISSSLALLYFGVIYIFLSTDPTAITSGTMQFYVFVAVG
                     TMVSHLSNELREQELRYRGIFDHSEAGVLLVDRKDHNIVEANEQSAALVGYPRSELAG
                     RHISAIWHDPADMETFFGRLQKEYAISGYETRFTAKDGGIRWVLISASILEDWEIVCT
                     VVDISERKQTEREIIRRNKELAIINQIGTALSTATTLEDLQKVALEKTLGFMDFDVGA
                     IYLVQNGGRTAELSHQQGIPEAVLPRVRELPADSPPYRRTLVEGHPLFYRDMAAAGID
                     WVRPPVVSLATIPLMTNVGIVGALFLGNTEPSSFYEGDTAILEHIGKEVGNAIAKARL
                     QMQLQEKIDEANLYIDIMTHDIHNANTISMGYAEILTEILKDEPKTFANKLLSSVIQS
                     SSIIDNVATIRRIHAERPVLRIKNLDDVIREEILHYPGAEIAYQPTGFSILADDLIPV
                     IFRNLISNSLKFGEQKGRVAISATAIGKDEVEVAVADTGPGIPDDLKAVIFARFQRGR
                     GKKSGKGLGLYITRIIVERYGGRIWAEDRVPGRPDQGAVVRFRLKRPA"
     gene            4135..4725
                     /locus_tag="VKO45_09175"
     CDS             4135..4725
                     /locus_tag="VKO45_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HMA06080.1"
                     /translation="MQVVAFNGSARKDGNTAILVRQVLSVLEKEGIRTELLQLAGERI
                     QGCNACGLCMKEQNRRCAVEDDSVNDCIARMDEAEGILLASPTYFADVSPAMKALMDR
                     AGFVAMANGGMFRRKVGAAVVAVRRGGAIHAFDTMNHFFFIGEMVVPGSNYWNVGIGL
                     DRGDVEKDEEGLRTMRVLGQNMAWVMKRLYVKEKAS"
BASE COUNT         1070 a         1391 c         1557 g          957 t
ORIGIN      
        1 aacgcggatg gaatatttcc atattgaatg tattcttcgt tcgctgataa atattgagag
       61 aataggatat atcggagaat gggtttggaa cgcccgaacg ggtagcttcc ttgctttccg
      121 gacgctttcg atcagggttt acctagcagg taatcagtga ttcagggatg cctgttgatg
      181 ataaactacc tgaagtcaaa tgagggatat taaatcagga gtgcgtatag tacagaagag
      241 gtctgatgcg ggatgggtaa gaggatcgtc atcaccggtg tcccgggcgt ggggaaaaca
      301 accgtcgtga acggggccct cgagaaactg gcacaggagg ggatttccta tcagtccatc
      361 aacttcggga ccttcatgtt cgaagtcgcc gcgaaccaga agatggtcag cgaccgggac
      421 gggatgagga ggctccccgg ggccgtccag aaggacatcc agcggaaggc ggcccaggcc
      481 atcgcaaaga tcgagggaaa catcatcatc gatacccact gcacggtgaa gaccccgaag
      541 ggattcctgg ccgggctccc cgaatgggtg ctccgggagc tcatgccgga catggtgatc
      601 ctcgtcgaga cggacgagga ccagatcctg tcccggaggc tcacggacac gacccgtacg
      661 agggacccgg actcatcgaa ggagatcgcc ctgcaccagg agtacaaccg ggcgatggcc
      721 gccgcctatg gcatggtgag cgggtgcacc gtgaagatcg tgaagaacca ggactttctc
      781 ctcgaccggg ccatcgggga catggccgac gcgctgaggt gaggtccgtg gcatcgaagt
      841 catcgggcag catgacctcc ctcgcactcg ccatggggct gatcttcctc tccaccatcc
      901 ccgccgtccg gcagttcgtg gggtccgcca tggatttcat cctcgggccc ctcctcgccg
      961 aacccttccc cggcctcccc ttctacgtcg tcatcctcat cctttcggcc cttaccgggc
     1021 tctactcctc ccttctccag aagtacacca tcgactatga ccgggtcctc tccgtccagg
     1081 agggaatgaa ggagttccag aaggaatacc gggacgccca gctctccaaa gacgagaaaa
     1141 ggattaaaaa gctcgaatcg aagagagata aaatgatgaa agagcagctg gaggtatccc
     1201 aggagcagtt caagcccatg gcctacatcc tcctcgtctc cattcccatc tttctctggc
     1261 tgatctacaa gatgccgacc ctctcgccgg agagctccgc aggcatcatc acgtttccct
     1321 acttcgggag cctggccatc tggaacgggg cgatctggat cgtgccggcc tggatcctgt
     1381 ggtacatgat ctgctccatc tccatcagcc aggtcatccg gaaatccatg aatatcgggg
     1441 gtatctgatg aagatcaccg tgagcggccc acccgggagc ggcacaacga cccttgcccg
     1501 cttcatcgca gagaagcaca agttcgagct catatcggcg ggagagttat tccgcgacct
     1561 cgcgacggag aagggcctct cggtggccga gttcggcgaa cttgccgaga cggacatcac
     1621 catcgatgcc ctcatcgatg tcaggcagaa ggagacggca aagacaaggg acaacatcgt
     1681 ggtggaggga cgcctctcgg ggtggatggt ggaggaggcg gatctccgga tctggctcaa
     1741 cgcccccatc gcctgccggg caaagaggat cgccacccgg gacgggatgg acgagtacac
     1801 ggcccgggag ttaaccctcc agcgggagga gagcgagcgg aggaggtaca ggaactacta
     1861 caagatcgag atagacgacc tctcgcccta tcacctggcc atgaactccg agctctggtc
     1921 cggggaggcc ctcgccgata tcgtggacct cgccatcggc catctgaaaa aataaaaata
     1981 attcctgttt tcccgtgaac tccgcctagg ccggtctctt caatcggaaa cggacgaccg
     2041 ctccctgatc gggacggccg ggaacccggt cctccgccca gatgcgcccc ccgtagcgtt
     2101 ctacgatgat ccgggtgata tagagcccga gtcccttccc gctcttcttc cccctgcccc
     2161 gctggaaccg ggcgaagatg accgccttca ggtcgtcggg gatgcccggg ccggtgtcgg
     2221 ccacggcgac ctccacctcg tccttcccga tggcggtggc gctgatcgca acccttccct
     2281 tttgttcccc gaacttgagg gaattggaga tcaggttcct gaagatgacc gggatgaggt
     2341 catcggcgag gatcgagaac cccgtcggct ggtaggcgat ctcggccccc gggtagtgga
     2401 ggatctcctc gcggatgaca tcgtccaggt tcttgatgcg gagcacgggc cgctccgcat
     2461 ggatccgccg gatggtcgcg acattgtcga tgatggatga gctctggatg acactcgaca
     2521 ggagcttgtt cgcgaaggtc tttggctcgt ccttcaggat ctcggtaagg atctcggcat
     2581 accccatgga gatggtgttc gcgttgtgga tgtcatgggt catgatatcg atgtacaggt
     2641 tggcctcgtc gatcttctcc tggagctgca tctggagacg ggcctttgcg atggcattcc
     2701 ccacctcctt cccgatgtgc tcgaggatgg cggtatcgcc ctcgtagaag gaggagggct
     2761 cggtgttccc gaggaagagg gcccccacga tccccacgtt cgtcatgagg gggatggtcg
     2821 cgagggagac gaccgggggc cggacccagt cgatcccggc cgccgccatg tcacggtaga
     2881 agaggggatg tccctcgacg agggtccttc ggtagggagg ggagtcggcg gggagctccc
     2941 ggacccgggg gagcaccgcc tcggggatgc cctgctggtg ggagagctct gcggtcctcc
     3001 ccccgttctg gacgaggtag atggccccca cgtcaaagtc catgaacccg agggtctttt
     3061 ccagggcgac cttctggaga tcctcgaggg tcgtcgccgt ggaaagcgcg gtcccgatct
     3121 ggttgatgat ggcgagctcc ttgttcctcc ggatgatctc cctctcggtc tgcttccgct
     3181 ccgagatgtc caccaccgtg cagacgatct cccagtcctc gaggatggat gccgagatga
     3241 gcacccaccg gatcccgccg tccttcgccg tgaaccgggt ttcgtacccc gagatggcgt
     3301 actccttctg gagccggccg aagaaggtct ccatgtcggc gggatcgtgc cagatcgccg
     3361 agatgtgccg gccggcgagc tcgctcctcg ggtacccgac gagggccgcg gactgctcgt
     3421 tggcctccac gatgttgtga tccttacggt cgacgaggag gacccctgcc tcggaatggt
     3481 cgaagatgcc ccggtaccgg agctcctgct cccggagctc gttggacagg tgggagacca
     3541 tggtcccgac ggcgacaaag acatagaact gcatggtccc cgaggtgatg gcggtgggat
     3601 cggtggagag gaagatgtag atcaccccga agtacaggag ggcgagggag gaggagatga
     3661 agatcccccg cttcgggtac tggaaggccg cgaagatcac cgggatgtag aagaagttcg
     3721 tgagcacgaa gacggtgccc tggtacaggt aatagatgaa gacgttcgcg atgaagacag
     3781 agaggagtac aaggccgatg agaaagaagt cccagaaggt ccactggtgg cggtcctgtt
     3841 tcatggtcat cagggatacc gtgggctctc cctgttgacc cccctgtagt aaacattttc
     3901 atcaaatagt atttagcctt tctcattcga aaacttgaaa atattacttc ataaaagtta
     3961 aatggagata ttttagtgac ataaggccct tcacccccga aaggcaaggg atccggccct
     4021 ttcatgttgc cccgagcatc accgttcacc cccggggaac ccgtcgggaa accgcgtgca
     4081 ggggaaggaa gcgattatgg gatggaagac cgataggaaa ggaggagaat cgccatgcag
     4141 gttgtcgctt tcaacgggag tgcccggaag gacgggaata ccgccatcct cgtgcgccag
     4201 gtcctttccg tcctcgagaa ggaggggatc aggacagagc tcctccagct cgcgggggag
     4261 agaatccagg gatgtaacgc atgcggcctg tgcatgaagg agcagaaccg gcgctgcgcc
     4321 gtcgaggatg acagcgtgaa cgactgcatc gcgaggatgg acgaggccga gggaatcctc
     4381 ctcgcctccc ccacctactt cgcggacgtg agcccggcga tgaaggccct catggaccgg
     4441 gcgggcttcg tggccatggc aaacggcggc atgttccgcc gcaaggtggg tgcggccgtg
     4501 gtggccgtcc ggaggggcgg ggccatccat gccttcgata ccatgaatca cttcttcttt
     4561 atcggcgaga tggtggtccc gggatcgaac tactggaacg tgggtatcgg cctcgaccgg
     4621 ggcgatgtag agaaagacga agaagggctc aggacgatgc gggtcctggg acagaacatg
     4681 gcctgggtga tgaagaggtt atatgtgaag gagaaggcca gttaaaaggc ggagagctcc
     4741 acatcttcct tctattgagt tgatctcttg ggcgggtgag cccccccggg agcccgatgg
     4801 tggggagaat ggataattgg aaaaggagga ttctctccag acctcccgaa tggtggacgc
     4861 tgaggggata acgaaaattg aatgatgaat ataccaacgg tctcactccg ctgtgaacca
     4921 cgaattttat tttgagaaag atataagagc ataagaaaat gatacgaaca aatga
//
LOCUS       DATGDI010000245         4755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_375953, whole genome shotgun sequence.
ACCESSION   DATGDI010000245 DATGDI010000000
VERSION     DATGDI010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375953"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VKO45_09180"
     CDS             complement(<1..262)
                     /locus_tag="VKO45_09180"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HMA06081.1"
                     /translation="MAAEAGLAAGLVLAAALGVDRVLGDPRSRFHPVAVLGRLIGWSG
                     DPGRWGLGSQRTAGVVCGLGTACLFALPFLLVDRFAPLWLFLL"
     gene            complement(241..750)
                     /locus_tag="VKO45_09185"
     CDS             complement(241..750)
                     /locus_tag="VKO45_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06082.1"
                     /translation="MAACEIHLNRRAINSIEIPAQADVEAGKSLILSFNNHGHPLHVS
                     LSAINAARYTPFTHENLFIQGETDFEIPILEDAGEGAFSLEVVTGYGRTSCEMRVFVR
                     RPQPEAPAEKPAEAPARRKIPWLRVGPVLLGLILYGASFLPDLGVLKDLAFFVLLAAL
                     LLAWPRKQG"
     gene            complement(753..944)
                     /locus_tag="VKO45_09190"
     CDS             complement(753..944)
                     /locus_tag="VKO45_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HMA06083.1"
                     /translation="MRRSLLDILCCPVCKGDLELRVTVEQAGEILEGVLACARCRVDY
                     PISDGIPDLIPREPEAGVG"
     gene            complement(941..1951)
                     /locus_tag="VKO45_09195"
     CDS             complement(941..1951)
                     /locus_tag="VKO45_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HMA06084.1"
                     /translation="MACTIIVGGFFGDEGKGKIVAHIAERDRPAIISRGGVGPNAGHT
                     VTVGEKSYGVRMVPSGFVYQDALLCIGTGVLVDPRVFLHEVELLGVKGRAFIDERCGI
                     IDQAHIDRDRGSDHLSKTVGSTGTGCGPANADRAMRVIRQAKDLPELSEYLMDVPAEI
                     NEALDDDDFVLIEGTQGFGISLYFGTYPYVTSKDTSASQMAADNGVGPTRIDDVIVVF
                     KAYPTRVGAGPFSTEMTPEEAQEHGIEEFGTVTHRRRRIGEWDSEMARYSAMINGCTQ
                     AAITGIDRIDPDCFGCTDYHKLSKAAKEFVKKAEEDIGAPVTLISTGPDISHIIDLRK
                     GP"
     gene            2125..2808
                     /locus_tag="VKO45_09200"
     CDS             2125..2808
                     /locus_tag="VKO45_09200"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HMA06085.1"
                     /translation="MKPVQTGGLEIALFQHMDSEGPGFFPDLFRARGMPFEIHRLYET
                     GEPPPDIRSPLVILGGGMSVHDEREFPFIATEKEIIRRYLREGRPVVGICLGAQLIAD
                     AAGSRVYPGAREFGWCGIARESPEHFRKCPDRMEVFQFHGDTFDLPGGAVLLWKGREV
                     KHQMFLLGSAAGVQFHPEITLPLIREWAKEHPARYIERLVAESSGKIPLSHAFCKELV
                     DRFVLRGLG"
     gene            2805..3578
                     /locus_tag="VKO45_09205"
     CDS             2805..3578
                     /locus_tag="VKO45_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HMA06086.1"
                     /translation="MKGIQGFLLDLDGVLYVGNQPVPGAVPALRSLREQGFPVRFVSN
                     STRRSRASVAGRLRAMGFPVDENEVLTPAVTAASMLANAGRTAFLVTMGDAHRDFEDA
                     GVALTGDHPDAVVVGDAGDRFTYGLLNRAMRMILSGADLIALERDRSWMGADGLMLSA
                     GPFVAALEYATGKEAICVGKPSHDFFSAALAGLGIPASGAAMVGDDIETDIGGAQRCG
                     MQGILVRTGKFREDILASSGICPDLIIDSVAELPEILRS"
     gene            complement(3575..4495)
                     /locus_tag="VKO45_09210"
     CDS             complement(3575..4495)
                     /locus_tag="VKO45_09210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HMA06087.1"
                     /translation="MRPEKVRDKPGKERIRKGTKDRRDRIPIVEPDGIRPFENRIICG
                     DAETVLHAFPAGSVDLVVTSPPYNFGHAYAGDPRDDTREWNEYFGRLAGVWRECYRVL
                     KPGGRIAVNIQPLFSDYIPTHHLVSKQLLDAGFLWKAEILWEKNNYNAKYTAWGSWKS
                     PSMPYLKYTWEFIEIFDKETHRKAGDRDDIDITVEEFKEWVMARWTFPPEHRMREHGH
                     PAMFPEELPRRVMKLFSYKGDLVLDPFNGAGTATRVARALGRRFIGIDLSREYCETAA
                     ARTFSRGKRGVSPSPAPEILVWDEATGKPG"
     gene            complement(4510..>4755)
                     /locus_tag="VKO45_09215"
     CDS             complement(4510..>4755)
                     /locus_tag="VKO45_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06088.1"
                     /translation="SLPHPFPMHGATIDSTFGFIAARAARYPVIVKKNQPRPILRDPA
                     RAMASGDPAIRWLLLNTQRINRFLEGTGKQGVAVTGS"
BASE COUNT          785 a         1513 c         1583 g          874 t
ORIGIN      
        1 caaggaggaa gagccagagg ggggcgaacc ggtcaacgag gagaaaggga agggcaaaga
       61 ggcacgcggt cccgagcccg cacacgacac cggcggtcct ctggctgccg aggccccagc
      121 ggccggggtc gcccgaccag ccgatgaggc gcccgaggac cgcgacgggg tggaaacggc
      181 tccgggggtc cccgaggacc cggtccaccc cgagggccgc cgcaaggacg aggcccgcgg
      241 ctagccctgc ttccgcggcc atgcgaggag gagggctgcg aggaggacga aaaaggcgag
      301 gtccttcagg acaccgaggt caggcaggaa ggacgccccg tagaggatga gcccgaggag
      361 gacggggccg acccggagcc agggtatctt cctgcgtgcg ggcgcctccg ctggcttttc
      421 cgcgggcgcc tcgggctgcg gtctccggac gaagacccgc atctcgcagc tcgtccggcc
      481 ataaccggtc accacttcga gggagaaggc cccctccccg gcatcctcga ggatgggaat
      541 ctcgaagtcg gtctctccct ggatgaagag gttctcatgg gtgaacgggg tgtaccgcgc
      601 cgcgttgatg gccgacaggc tgacgtggag ggggtggccg tggttgttga aggaaaggat
      661 gaggctcttc cctgcctcga catcggcctg ggcagggatc tcgatggagt tgatggctct
      721 ccggttcagg tggatctcac acgccgccat gttcatccca cccctgcttc gggttcccgg
      781 gggatgaggt cggggatccc gtcgctgatg gggtagtcca cccggcaccg ggcgcatgcg
      841 aggacaccct ccaggatttc accagcctgt tcaacggtga cccggagctc gagatctccc
      901 ttgcagaccg ggcagcagag gatatcgagg agggatcgcc tcatggtccc ttcctcaggt
      961 cgatgatgtg ggagatgtcg ggcccggtgg agatgagggt cacgggtgcg ccgatgtcct
     1021 cctcggcctt cttcacgaac tccttcgcgg ccttcgagag cttgtggtag tcggtgcagc
     1081 cgaagcagtc ggggtcgatc cggtcgatgc ccgtgatggc ggcctgggta cagccgttga
     1141 tcatcgccga gtacctggcc atctcactgt cccactcccc gatcctcctc ctccggtggg
     1201 tcaccgtccc gaactcctcg atcccatgct cctgggcctc ctcgggggtc atctcggtgg
     1261 agaaggggcc cgccccgacc cgcgtcgggt aggccttgaa cacgacgatg acgtcatcga
     1321 tcctcgtggg cccgaccccg ttgtccgccg ccatctggga ggccgaggtg tccttggagg
     1381 tcacgtacgg gtaggtcccg aagtagaggg agatgccgaa cccctgggtg ccctcgatga
     1441 gcacgaagtc gtcgtcatca agggcctcgt tgatctcggc cgggacgtcc atgaggtact
     1501 ccgagagctc ggggagatcc tttgcctgcc tgatgacccg catggcccgg tcggcgttgg
     1561 caggcccgca gccggtcccc gtggagccta cggtcttcga gagatggtcg ctcccccggt
     1621 cccggtcgat gtgggcctga tcgatgatcc cgcaccgctc atcgataaag gccctcccct
     1681 ttacccccag gagctcgacc tcgtggagga acacccgggg atcgacgagg accccggtac
     1741 cgatgcagag gagggcgtcc tggtacacga agcccgaggg caccatgcgg acgccataag
     1801 acttctcacc gacagtgacg gtgtgcccgg cgttcgggcc gaccccgccc cgggagatga
     1861 tggccggccg gtcacgctcg gcgatgtgcg ccacgatctt ccccttcccc tcgtccccga
     1921 agaaaccccc cacgatgatt gtgcaggcca ttgttcgatc atatagagta cgggatgatg
     1981 gaattaaagg cttctgatgc gggaacgtca aggcgatccc ccttctccga cgaagaaatg
     2041 gaggggacgg ttctcccggt tcctctccct tgttttcccc cgggggagaa gatattagac
     2101 cgttcatccc caaccctggc atgtgtgaaa cccgtgcaga ccgggggtct cgagatcgcc
     2161 cttttccagc acatggattc cgaggggccg gggttctttc cggacctttt cagagcccgg
     2221 ggcatgccct ttgagatcca ccggctgtac gagaccggcg agcccccgcc ggatatccgc
     2281 tctcctctcg tcatcctggg gggcggcatg tcggtccacg acgaaaggga gttccccttc
     2341 atcgccacgg agaaggagat aatccggcgg tacctccgcg aggggaggcc cgtggtgggg
     2401 atatgcctcg gggcgcagct gatcgccgat gcggcgggct cccgggtata tcccggggcc
     2461 cgggagttcg gctggtgcgg gatcgcgagg gagagcccgg agcattttcg aaaatgcccc
     2521 gaccggatgg aggtcttcca gttccacggc gataccttcg atctccccgg gggggcggtc
     2581 cttctctgga agggccgtga ggtgaagcac cagatgttcc tcctcgggag tgctgcgggc
     2641 gtccagttcc accccgagat cacgctcccc ctcatcaggg agtgggcgaa ggagcatccc
     2701 gcccggtata tcgagaggct cgtcgccgaa tcctccggga agatccccct ctcccatgcg
     2761 ttctgcaaag agctggtgga ccggttcgtc ctccgggggc tgggatgaag ggaatccagg
     2821 ggtttcttct ggacctcgac ggggttcttt acgtcgggaa ccagccagtc ccgggggccg
     2881 tcccggccct ccggtctctg cgagaacagg gcttccctgt gcggttcgta tccaactcga
     2941 cacggcggtc ccgagcctcg gtggccggcc ggctcagggc gatggggttc cccgtggatg
     3001 aaaacgaggt actcacgccg gccgttaccg cggcgtccat gctcgcaaat gcagggagga
     3061 ccgcgtttct cgttacgatg ggcgatgccc accgggactt tgaggacgcg ggcgttgcgt
     3121 tgaccggtga tcatcccgat gccgtggtcg tcggggatgc gggcgaccgg ttcacctacg
     3181 ggcttctcaa ccgcgccatg aggatgatcc tctcgggggc agatctcatc gccctcgagc
     3241 gggaccgcag ctggatgggt gcggacgggc tcatgctctc ggccggtccc ttcgtcgccg
     3301 cactcgagta tgcgacggga aaggaggcga tatgcgtggg aaagccatcc catgatttct
     3361 tctccgccgc tctcgccggc ctcggaatcc ctgcctcagg agctgccatg gtcggcgatg
     3421 atatcgagac cgacatcggc ggggcgcagc ggtgcgggat gcaggggatc ctggtcagga
     3481 ccgggaagtt ccgggaagat atcctcgcga gctcggggat atgcccggac ctcatcatcg
     3541 attccgtcgc cgaacttcct gaaatcctcc gatcttagcc gggtttgccg gtcgcctcgt
     3601 cccagacgag gatctccggg gctggagagg gtgacacccc cctcttcccc cgggaaaaag
     3661 tccttgcggc cgccgtttcg cagtactccc gggagaggtc gatcccgatg aaccggcggc
     3721 cgagggcccg ggcgacccgg gtcgcggtcc ccgccccgtt gaaggggtcg agaacgaggt
     3781 cccccttgta cgagaagagc ttcatcaccc gccgggggag ttcctcgggg aacatggcgg
     3841 ggtggccgtg ctccctcatc cggtgctcgg gggggaaggt ccacctcgcc atcacccact
     3901 ccttgaactc ctcgacggtg atgtcgatgt cgtcccggtc ccccgccttc cggtgggtct
     3961 ccttgtcgaa gatctcgatg aactcccagg tgtacttcag gtagggcatg gaaggggatt
     4021 tccaggatcc ccaggccgtg tacttggcat tgtagttgtt cttctcccag aggatctccg
     4081 ccttccagag gaaccccgca tccaggagct gcttcgagac gaggtggtgg gttgggatgt
     4141 aatcagagaa gagaggctgg atgttcaccg cgatccgccc gccgggcttc aggacccggt
     4201 aacactccct ccacaccccg gcaaggcgcc cgaagtactc gttccactcc cgggtgtcgt
     4261 cacgggggtc tcccgcgtac gcgtggccga agttgtacgg aggtgaggtg acgaccaggt
     4321 caacgcttcc cgccggaaac gcatggagga ccgtctccgc atccccgcag atgatccggt
     4381 tctcgaaggg gcggatccca tcgggctcca cgatagggat ccgatccctc cggtccttgg
     4441 tgcctttccg gatccgctcc ttccctggct tgtcccggac tttttcaggc ctcatgccgc
     4501 cggttttctt caggaaccgg ttaccgcgac cccctgcttc ccggttccct ccagaaaccg
     4561 gttgatccgc tgagtgttta acagtagcca ccggatggcc gggtctcccg acgccatcgc
     4621 ccgggccgga tccctcagga tcgggcgcgg ctggttcttt ttcacgataa ccgggtaccg
     4681 ggccgccctc gcggcgatga acccgaaggt tgaatcgatg gttgctccgt gcatgggaaa
     4741 ggggtgagga aggga
//
LOCUS       DATGDI010000246         5033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_27844, whole genome shotgun sequence.
ACCESSION   DATGDI010000246 DATGDI010000000
VERSION     DATGDI010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27844"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VKO45_09220"
     CDS             <1..200
                     /locus_tag="VKO45_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="eL32 family ribosomal protein"
                     /protein_id="HMA06089.1"
                     /translation="NEAMLAELDPKAHAIRIAAGVGMKKKMEIQRKAMDAGLKVLNPR
                     QAPAPGEEPEAEPAEEASSDE"
     gene            193..645
                     /locus_tag="VKO45_09225"
     CDS             193..645
                     /locus_tag="VKO45_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HMA06090.1"
                     /translation="MSNLTNQKRLAASVLGCGVNRIWFDPERATDVEGAISRDDLRTL
                     ITEGVIKARPKTGVSRGRARERDAKRSYGHRRGPGKRRGAAGARRPSKTQWIQRIRAI
                     RRTMRALRDEGKIDPHLYRILYRKAAGGQFRSVAHLKSQVDLLQGKGK"
     gene            647..1174
                     /locus_tag="VKO45_09230"
     CDS             647..1174
                     /locus_tag="VKO45_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HMA06091.1"
                     /translation="MSTGPRYFIPFRRRREGRTDYYARGRLLLSENPRMVVRKTNREI
                     IIQLMVPDPTGDRTLVAAYSRDLPGYGYTGSRSNTPAAYLTGILFGARCRKAGYGSAV
                     LDIGLARASPGARVFAALKGAVKAGLSIPHSTEVLPDDSRVRGEHIAGFAPQRAGELS
                     ENVDKVAEAIMKEVS"
     gene            1177..1794
                     /locus_tag="VKO45_09235"
     CDS             1177..1794
                     /locus_tag="VKO45_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HMA06092.1"
                     /translation="MAEIEGWIPLTGLGKMVASGQITSIDQVLESGKPIKEVQIVEAF
                     LPDLADEVLDITMVQRMTDSGRRVKFRTVVVVGNRNGYIGFGQGRDAQVGNAITKAIK
                     KAKLNIIKVNRGCGSWECGCNLEHSIPVQVTGKAGSVRVTLKPAPQGIGLVTGDIAKK
                     VLALAGIRDVWGFSSGETRTTINFAKATFQALQQTNMVRRAAGVR"
     gene            1795..2256
                     /locus_tag="VKO45_09240"
     CDS             1795..2256
                     /locus_tag="VKO45_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HMA06093.1"
                     /translation="MYAVVQVRGTVKTRREIKDTLAMLRLHHINHCVVIPDTPEYLGM
                     IRKVKDFVAYGEVDAEILGTVLSTRGRLVGNRPLTDEYVKANSGFASIEEFARALAKG
                     ETSLRDLPGLKPVMRLHPPRKGYRTLKRTFQQGGALGYYGKEINTLLYKMR"
     gene            2264..2686
                     /locus_tag="VKO45_09245"
     CDS             2264..2686
                     /locus_tag="VKO45_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HMA06094.1"
                     /translation="MPSNKRSKYRGSRTCGGGTHKNRRNGGSRGGRGFAGGCKHHFVR
                     YYLMGRTFGKDGFSHDQKFDPATIDVGAIEVMAEALLARGLAKMEGDTLTIDAGAFGV
                     GKILGSGRVTRKMKVSAEAFSEAAKKKLEARGGQALVV"
     gene            2704..4137
                     /gene="secY"
                     /locus_tag="VKO45_09250"
     CDS             2704..4137
                     /gene="secY"
                     /locus_tag="VKO45_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HMA06095.1"
                     /translation="MGAILDRMEPLLAAMPSVKTPEGHVHFKTKLFWTVGVLILYFIL
                     SNILIFGLAPTSQDIFLYWRALLAGQSGTIIHLGIGPIVTASIVLQLLKGADLLQIDT
                     SETRGQVIYMGLQKLLIFVMIVVEAIPNLIGGFMVPDPTIAARFFGGNTGLLAFLIFL
                     QLCVGGVLIFLLDEVVTKYGIGSGVGLFIIAGISQSIMNGFLSWEAIQDPYPVGFFPR
                     LFAIGLQGGSYLQYFTPNLIAFITTIAIFLIIVYVESTRVEIPLAHTNVRGIRARFPV
                     KLIYASVLPMILVRVLQANIQMIGLFLNNVGITIFGTFQSQTAVSGLMYFLAPINGPS
                     DWMWWTTDLGHPVWQVLLRMGIDITIMVVGGAIFALFWIKTSGLDSKDVARQIQLSGM
                     HIPGYRKNPQVLERYLDRYIPRITIIGGVFIGVISIVANLFGVIGRESGTGLLLTVSI
                     TYRLYEEIASGQLMEMYPAMRQFFGKE"
     gene            4270..>5033
                     /locus_tag="VKO45_09255"
     CDS             4270..>5033
                     /locus_tag="VKO45_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA06096.1"
                     /translation="MTKKDLVKEFPDVLDSAYKDYKRHQKIADIMSRNVTTTTTDTPM
                     DEAAKIMGEKHIGSLIVEKYGIPIAIVTERDLLTKVIAGKRSIGETKVEEVMSYPLVK
                     VCPTMEIRAAAQTMIQRKGRLAVFECGDLVGIITASDLIRGMPDAPETTLRVDDFMSK
                     KVVTVDEEATVARVAEMMGSARIGSVLVTRKKRPRGIFTERDLLSKFLAKERPLDVAV
                     GEVSSSPICTSKAGISIHEAAKLMTSKHIRRLPIIK"
BASE COUNT          999 a         1600 c         1607 g          827 t
ORIGIN      
        1 acaacgaggc gatgctcgcg gagctcgacc cgaaggcaca cgcgatccgc atcgccgccg
       61 gcgtcgggat gaagaagaag atggagatcc agagaaaggc gatggacgcg ggcctgaagg
      121 tgctgaaccc gcgccaggct ccggctcccg gggaggaacc ggaggcagag cccgcagagg
      181 aggcgagcag cgatgagtaa tctcacgaac cagaagaggc tcgcggcgag cgtcctcggc
      241 tgcggggtga accggatctg gttcgacccc gagcgggcca cggacgtcga gggagccatc
      301 tcccgcgacg acctgcgcac cctcatcacg gagggcgtga tcaaggcccg gccgaagacc
      361 ggggtctccc ggggcagggc ccgggagcgg gacgcgaagc gttcctacgg gcaccgcagg
      421 ggcccgggga agaggcgggg ggcggcaggc gcccggaggc cctcgaagac ccagtggatc
      481 cagcggatcc gggccatccg aaggacgatg cgtgccctca gggacgaggg aaagatcgac
      541 ccgcacctgt accggatcct gtaccggaag gccgcgggcg gccagttccg gagcgtggcc
      601 cacctgaaat cccaggtgga tctcctccag gggaagggga agtgacatga gcaccggacc
      661 acgctatttc atccccttcc gcaggcgccg ggagggccgg accgactact atgcccgggg
      721 caggctgctc ctctccgaaa acccccggat ggtggtacgg aagacgaacc gggagatcat
      781 catccagctc atggtcccgg acccgacggg ggacaggacc ctcgtcgcgg cctactcccg
      841 ggacctcccg ggctacgggt acacgggctc aagatcgaac accccggccg cctacctgac
      901 cggcatcctc tttggtgccc ggtgcaggaa ggcggggtac gggagtgccg tcctcgacat
      961 cggcctcgcc cgtgcctcgc ccggggcccg ggtctttgcc gccctcaagg gtgcggtgaa
     1021 ggcgggtctc tccatcccgc acagcaccga ggtgctgccc gatgacagcc gggtcagggg
     1081 agagcacatc gcgggcttcg ccccccagcg ggccggggag ctcagcgaga acgtggacaa
     1141 ggtggccgag gccatcatga aggaggtgag ctgatcatgg cagagatcga ggggtggatc
     1201 cccctcaccg gcctgggaaa gatggtggcg agcggccaga tcacctccat cgaccaggtc
     1261 ctcgagagcg ggaaacccat caaggaggtc cagatcgtgg aggccttcct ccccgacctc
     1321 gcggacgaag tgctcgacat cacgatggtc cagcggatga cggactcggg ccggcgggtg
     1381 aagttccgca cggtcgtcgt tgtcgggaac cggaacgggt acatcggctt cggccagggc
     1441 agggacgccc aggtggggaa cgccatcacg aaggccatca agaaagcgaa gctgaacatc
     1501 atcaaggtga accggggctg cggctcctgg gagtgcggct gcaaccttga gcactccatc
     1561 cccgtccagg tgacggggaa ggcgggctcc gtccgggtca ccctgaagcc ggccccccag
     1621 gggatcggcc tcgtcacggg cgacatagcg aagaaggtgc tcgccctcgc cgggatccgg
     1681 gacgtctggg gattctcttc gggggagacc cggaccacca tcaacttcgc aaaggcgacc
     1741 ttccaggccc tccagcagac gaacatggtc cgccgggcgg cgggggtgcg gtaaatgtac
     1801 gccgtcgtgc aggtcagggg caccgtaaag acccggcggg agatcaagga caccctcgcc
     1861 atgctccggc tccaccacat caaccactgc gtcgtgattc cggacacgcc ggagtacctc
     1921 ggcatgatcc ggaaggtgaa ggacttcgtc gcctacggcg aggtggacgc ggagatactc
     1981 ggaaccgtcc tctcgacccg ggggaggctc gtaggaaacc ggcccctcac ggacgagtac
     2041 gtgaaggcca actccggctt tgcatccatc gaggagttcg cccgggcgct cgcgaagggg
     2101 gagacatccc tccgggatct cccgggcctg aagccggtga tgcggctcca cccgccccgg
     2161 aaggggtacc ggaccctcaa acggaccttc cagcagggcg gtgccctcgg gtattatggc
     2221 aaggagatca atactctcct gtacaagatg aggtgaccac ggaatgcctt cgaacaagcg
     2281 atccaagtac cggggatccc gcacctgcgg gggcggcacc cacaagaacc gccggaacgg
     2341 cggctcgcgg gggggacggg gcttcgcggg cgggtgcaag caccacttcg tccgctatta
     2401 cctcatgggg aggaccttcg gaaaggacgg gttctcccat gaccagaagt tcgaccccgc
     2461 gaccatcgat gtcggggcca tcgaggtcat ggccgaggcc ctcctcgccc ggggcctcgc
     2521 gaagatggag ggggacaccc tcaccatcga cgcgggcgcc ttcggggtgg ggaagatcct
     2581 cgggagcggc cgggtgaccc ggaagatgaa ggtctcggca gaggccttct ccgaggcggc
     2641 aaagaagaag ctcgaggccc ggggcgggca ggcgctcgtc gtctagagcg ccccggtgaa
     2701 aacatgggag ctatcctcga ccgcatggag ccgcttctcg cggccatgcc ctcggtgaag
     2761 accccggagg gacacgtcca cttcaagacg aagctcttct ggaccgtcgg ggtcctcatc
     2821 ctctacttca tcctctcgaa catcctgatc ttcgggctcg cccccacctc ccaggacatc
     2881 ttcctctact ggcgggccct cctcgccggg cagagcggca ccatcatcca cctcgggatc
     2941 gggcccatcg tgaccgcctc catcgtcctc cagctcctga agggcgccga tctcctccag
     3001 atcgacacct cggagacgcg ggggcaggtc atctacatgg ggctccagaa gctcctcatc
     3061 ttcgtcatga tcgtcgtaga ggccatcccg aacctcatcg gggggttcat ggtgcccgat
     3121 ccgaccatcg cggcccggtt cttcggcggg aacacgggcc tcctcgcctt cctcatcttc
     3181 ctgcagctct gcgtcggcgg ggtcctcatc ttcctcctcg acgaggtggt gacgaagtac
     3241 gggatcggct ccggtgtcgg gctcttcatc atcgccggga tctcccagtc catcatgaac
     3301 gggttcctct cctgggaggc catccaggac ccctacccgg tggggttctt cccccggctc
     3361 tttgccatcg gtctccaggg aggcagttat ctccagtact tcacgccgaa cctcatcgcg
     3421 tttatcacca ccatcgccat cttcctcatc atcgtgtacg tggagtcgac gagggtcgag
     3481 atccccctcg cccacacgaa cgtccgaggc atccgggccc gcttccccgt gaagctgatc
     3541 tacgcgagcg tgctcccgat gatcctcgtc cgggtcctcc aggcgaacat ccagatgatc
     3601 ggcctcttcc tgaacaacgt cgggatcacc atcttcggga ccttccagtc gcagaccgcg
     3661 gtctcgggcc tcatgtactt cctcgccccc atcaacggcc cgtcggactg gatgtggtgg
     3721 accaccgacc tcgggcaccc cgtctggcag gtcctcctcc ggatggggat cgacatcacc
     3781 atcatggtgg tcgggggtgc catcttcgcc ctcttctgga tcaagacctc gggcctcgac
     3841 tcgaaggacg tggcgaggca gatccagctc tcggggatgc acatccccgg ctaccggaag
     3901 aacccccagg tcctcgagcg gtacctggac cggtacatcc cccggatcac catcatcggc
     3961 ggtgtcttta tcggggtcat ctccatcgtg gcaaacctct tcggggtcat cgggcgggag
     4021 agcgggaccg ggctcctcct caccgtttcc atcacctacc ggctctacga ggagatcgcc
     4081 tccgggcagc tcatggagat gtacccggcg atgcggcagt tcttcgggaa ggaataaatt
     4141 tcacccgctt acattttttc cggaattccc gactccattc ctcattccat ctgtgaaggc
     4201 tatctcactt ccgggggagc gaaaagtata atgcatcatg cccactctct acatgcgagg
     4261 gtgaataggg tgacgaagaa ggacctcgta aaggaattcc cggacgttct cgatagcgct
     4321 tataaagact ataagaggca ccaaaagatc gccgacatta tgagcagaaa cgtcacgacg
     4381 accacgacag acaccccgat ggatgaggcc gcaaagatca tgggggagaa gcatatcggg
     4441 agcctgattg tggaaaaata cggcataccg atcgccatcg tcaccgagag ggatctcctg
     4501 acgaaggtga tcgcagggaa aaggagcatc ggggagacga aggtcgagga ggtgatgtcc
     4561 tacccgctcg tcaaggtatg tccgacgatg gagatcaggg cagctgcaca gacgatgatt
     4621 caaaggaagg ggaggcttgc ggtcttcgag tgcggggatc tcgtgggaat catcaccgcc
     4681 tcagatctca tccgcggcat gcctgacgca cctgaaacta ctcttcgcgt ggatgatttc
     4741 atgtcaaaaa aggtcgtgac cgtcgacgag gaagcaacag ttgcccgggt ggcagagatg
     4801 atggggagtg cgaggattgg gagcgtcctc gtcacccgca agaagcgacc gcgggggatc
     4861 tttaccgaac gggatctcct gtcgaagttc ctcgcaaaag agaggcctct cgacgttgcc
     4921 gtgggcgagg tttcctcatc accgatctgc acgtcaaaag cgggtatcag cattcacgag
     4981 gctgccaaat tgatgacatc aaagcacatc cgaagacttc ccatcatcaa gga
//
LOCUS       DATGDI010000247        20192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_231168, whole genome shotgun sequence.
ACCESSION   DATGDI010000247 DATGDI010000000
VERSION     DATGDI010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 20192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20192
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231168"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..385
                     /locus_tag="VKO45_09260"
     CDS             2..385
                     /locus_tag="VKO45_09260"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMA06097.1"
                     /translation="MDIKKKPVDTIDEYIAQFPREIQERLQKLRKTIREEAPDAEETI
                     SYRIPTFRLNGNLVHFAAFKDHVSFFPTSSGVGAFRQELSGYKISKGTIQFPLDEPMP
                     LDLVRKIVRFRVRDNLEKGKPGKKQ"
     gene            456..1466
                     /locus_tag="VKO45_09265"
     CDS             456..1466
                     /locus_tag="VKO45_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06098.1"
                     /translation="MKASHFLLVLLLALAVPAASALVTQSGGTVRIAQATDDDVFATG
                     GMVTIDAPVGSLMAAGGQIIVNAPVKGDVIAAGGQVTINSDVGGKVVAAGGSVTVNGK
                     VGTNAVLAGGTIDLGTKATIGRDAMVSGGSVTNAGTVTGKLSVRANTFENRGTAGTLE
                     VELRQRSTLGGTLLSVFGILFTIGLFILGLILIRLAPGRYHAVEDELARSPVVKTVGG
                     FIALIIGFIVILLLAITLVGLPIAILLGLIYLAGVLVSTLFVSSVLGRRVALLLKHPA
                     SDYVAFTIGFVILAVLFRIPLVGTLILVVAVSLGFGAVMFTLWKNRACICSPAAPPAG
                     QP"
     gene            complement(1501..2364)
                     /gene="thiL"
                     /locus_tag="VKO45_09270"
     CDS             complement(1501..2364)
                     /gene="thiL"
                     /locus_tag="VKO45_09270"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HMA06099.1"
                     /translation="MDDRDLLESILDIVGDRARDDCAVLPLGDRIIVATTDMLHTKTD
                     FLEGMTDWQCGWMAVAVTLSDIASMGAAPRWILLAVGLDRAERLRPVMVGAKDCCDRF
                     GAELVGGDIDSHDELTLVSTGLGTVEPEHLVRRKGSRVGDLVCVTGVLGRAQAAIDGH
                     TQYRKALFEPQPRVREGQILGRSGATSMMDISDGLALSLYDLTLANPCGYRITSAMIP
                     VVEDIPYEKALELAVYGGGDYELLFTIPAEKLPLEGMTYHVIGHVVGEPGAFLNGVPM
                     ERRGYQHRWKE"
     gene            complement(2357..4309)
                     /locus_tag="VKO45_09275"
     CDS             complement(2357..4309)
                     /locus_tag="VKO45_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF460 domain-containing protein"
                     /protein_id="HMA06100.1"
                     /translation="MKVFGIDVVKGSVRSRTRRPVYALVRMEDGDVTGEGEVTQFRLL
                     REISAEEPDILAVDSLQEIARDQHELFAFLEHLPPRTRLIQVTGGERPETLGKVAARY
                     NISFDRFDPFAEARAAAKIASLGGGAEVVAWENSCLVVVSRNRSLGKGGWSQNRYVRK
                     VHGAVLREAREIEERLVQAGLRYEKRESRAFGGLSRVQFLVHAPREEIPVSSRRGGDV
                     QVRVSGQRLDRIRFKPAGKGAGYLIVGIDPGTTTAVAAVDLEGNLLHLSSGRQRSMAD
                     IVETLYQAGKPLVVASDVSVMPFSVERVRRAFSAVPFTPREDRTGEEKQALTEGFSYG
                     NLHERDALSAALDAYRSYRNRFQSILKRVPPGFDLARVKAGIIRGQSVEQILGELSPA
                     RAPAGEKPPEPVVPGRQDERVMVLDGMVKRLRSLVAELQEEVKARETEISRYSERLDR
                     ERSRSFLRMKRDAEITRRDAVIRDLRHRLRRAERQVKSLRGRLEREKEAEEVRVEGSR
                     LPLKILPSLTRDGVQALVQEAGIAEGDLLYVARSDGWGRSVVKDLAGLGVTAVLVGVS
                     PPGRPDPLLLDTSREEEIPVLPVPAGEVQMRGPAGSVARDVVDRALANWEEDREKDRI
                     EREKAKVEYLFREYRTEREKEVRKGG"
     gene            complement(4306..5163)
                     /locus_tag="VKO45_09280"
     CDS             complement(4306..5163)
                     /locus_tag="VKO45_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HMA06101.1"
                     /translation="MPISAEAVRNLQENDLLVLHTLERLMKRFQWVPLDVLAHATGKG
                     EKEVSYRLTRLLQFGMAKYEKVPYEGYSLVFTGYDALALHALSGKGTVSALGPFIGEG
                     KESIVYEGLGLGRLVLKFHRIGQRSFQSARLKRGYMPEAGHVPWLFASAFSAEREFLA
                     LTKLHPKVSVPVPIERNRHVVVMSLIPGEILSRCGLEHPRAMLDAILVQVREAYRLGV
                     VHADLSEFNVMIEGERCFLIDWPQWMEATHPNAGEILERDLRVILLYFERKYGIEYPL
                     SRAFGEVTG"
     gene            5219..6010
                     /locus_tag="VKO45_09285"
     CDS             5219..6010
                     /locus_tag="VKO45_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)-lysidine synthase"
                     /protein_id="HMA06102.1"
                     /translation="MRCDRCDRDAILLQRYSGLRLCGEHLISGLESRAKGTIRAHGWI
                     RPGDRIAVALSGGPASVSLLHFLVTHFGMRRDLSFIAITVDEGGWTDLGRTRAIAEGM
                     GIEWAAASPHREIRGDRVPGQTEAPFGGTGLRDDALASLARDGGATKLALGTSLDSMA
                     RSVFLKVLDGDGAGLTRGSGEGEGGIPRIEPFIRIPAGELALYAMLNSLDHVSGRGMD
                     ARDSLEAEAGRLLDDFAARHPSAPFSLANLGEALSGGTGGGRTHG"
     gene            6003..7706
                     /locus_tag="VKO45_09290"
     CDS             6003..7706
                     /locus_tag="VKO45_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HMA06103.1"
                     /translation="MAEGIPPWRFIFGQGRRGIPLLAGIFILALIFFTAIFHTIYPIF
                     EGREISWAQALLFILETVSTTGYGDLLPFRSDYTMLFAIVLILSGVFMIFMFIPVLLE
                     PMLTRIISAPPPRGTKREMKGHVVIAGYGPLARALIDSLIISDLGIVVVEADERVARE
                     VVSHYPSRVSVIWGDYRAPRTWEQAWVRHAHSVVVCEDERTAAEIILGVRDLTAGSII
                     AVVDDLAYDRYLRYAGAEYVLSPKNSTGKILARHAVIRPDVDTIFEAISLDHMRLEEG
                     RSADGSLKLVKVPVMPGCIAFGKSLGELSLFERYGVDLLFLWKAGEFVPSPQREDLLD
                     ASTMLFLLGKASSVSEVLDREFCIRETGEPSAVISGYGDVGKAAYAELTGAGVRCTVI
                     DRRSHGVSEVVGSAEDEAVLRAAGVGEARFVIAAVNDDSVNIFTTLVARNLNPSARIL
                     ARASEPASVEKLYKAGADYVALLPTIGGQVIAGIILSDIVRVLVDLPNGQKVLMKHLT
                     RHAGATVGFLERRTGVRIVGLEGPGRSVVRPGPGEPVGDGDSIIAVGGNRELKRLIHY
                     I"
     gene            complement(7747..8433)
                     /locus_tag="VKO45_09295"
     CDS             complement(7747..8433)
                     /locus_tag="VKO45_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HMA06104.1"
                     /translation="MIPWDGPLPAPRLRTIEEIRGVLMDPACGCAEPLYSMYRDLSKS
                     PEDRRWLREQDLRYDLTVIPARTLCGECVKTKGHHHPANGKGVPYPEIYEILEGEAHY
                     LLQTRSAEDVLLVRAGPGDRVLVPPGYGHVTINPGRSTLSMANIVSTRFESDYSVYEK
                     FGGGAWFELEGGALVRNPRYPHAAGARILSARDQKGLDFLPRRPLYDLVGTKSLGFLN
                     RPEEYPEIFS"
     gene            complement(8433..9728)
                     /locus_tag="VKO45_09300"
     CDS             complement(8433..9728)
                     /locus_tag="VKO45_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HMA06105.1"
                     /translation="MQGRSLPPLIAIIALLALLAAPALAGEEQLFGSPQLSAAISGTN
                     EFTPGEDVTLTVKVQNTGVNEVTITQSTIITPIDKPNTAKLLTVGLSSGTAPVTVKTD
                     PQMVGDLTGGSSVAATFSVKISKYAKGGVYSLPLQLNYQYLNTAEKVSFDTLRYWYYT
                     TNITIPLDIRIKPGVTLDPVMSRADTLNVGTEGYIVVTITNAGSETARDAVAKLTRDG
                     LSPLIPTDASVYLGDFGPGDTREIRFRASVSADAEAQSYPVDVHVEYTDSEGESASSD
                     TVTIGVPVGSKIDFAVVSPVPQVNPGQTVTLEVTYRNTGSAAVHAAEARISLVDPFTS
                     SDDTAYLGDLAPGESGTALYDVTVASGATPKSYGLDSEIRYRDALDNSQISDTIKVQV
                     EVVPSQGILGILGNPLVLAVLVVLIGAVLVYIFIQRKKK"
     gene            9794..10564
                     /locus_tag="VKO45_09305"
     CDS             9794..10564
                     /locus_tag="VKO45_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMA06106.1"
                     /translation="MEMGEDLRAALPQILKSLGFTKYEALVYVALLGMEGATATEIHE
                     FSRVPRASVYPVLDRLVEKNLVTVSHATPKRFSAIPPEEGIANLLSRIEEDALRAREI
                     LGDIYRKRVTVERGDQELIWSIYGEEKIATRLRDLIRGAKRRVQLFTTWPFLERVLLP
                     ELVQLSPPVEVEVVTGHWEGPVPPGMKVHVRPLRGHPERVPMETAGIFIIDGKRAMVI
                     MGVREEAPTAIFSESPGFLRFFSQILAMNLAWASGPDQ"
     gene            complement(10639..11421)
                     /locus_tag="VKO45_09310"
     CDS             complement(10639..11421)
                     /locus_tag="VKO45_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF128 domain-containing protein"
                     /protein_id="HMA06107.1"
                     /translation="MEPENPVNPCEPCCPSLAFVSNRIEDLAMEVTYNPVENTGKIIF
                     NLSLIPDDRLEVAITLFRNAYRAGLSVSDRVRILDAGTKLEGLTIPEGLNGICTLCTI
                     TLDGILLRRGVPIQPVGGGLVEIVDRVPRRFTHMILYEATTIDPLEVLVAQDLTSITR
                     VMKEGSGTILANLRECHMEAESLVMGLLDDLSEVGFTGILEVGAPNVPILAVPVSPQY
                     FGVAMVGGTNPMAALKEAGIPVVTKALKGVMDVREMGSIRDY"
     gene            11564..12496
                     /locus_tag="VKO45_09315"
     CDS             11564..12496
                     /locus_tag="VKO45_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06108.1"
                     /translation="MDSEMRDRAREAVRRILEAASYEVEDLERPLDLSAIGDGECVIV
                     QCSDNTGEIAQFDRTNYRCRVEEKEVTSRKLLFTLNDSAVTGHCVRWGIRELGEFAGR
                     AAMATVLGERLSLDLGAGAAEASTPAIDQAGPELPHIPVKVDEKRARAISGESGAGTL
                     RFIPYWRYHYRSTGQKGYGNQVVTFNAEKKGAINAINGAETGEELGQVQVSPVPVSAV
                     VLQPMVQKSEAEEKLRDRVVEELTQKVRIKQVKGDTIFYEERIFKPDRKDIAIELELV
                     HLPVWQFRGKKFLELNAVTGVVLTEPMDTGAELL"
     gene            12504..13832
                     /locus_tag="VKO45_09320"
     CDS             12504..13832
                     /locus_tag="VKO45_09320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMA06109.1"
                     /translation="MILVIGGGPAGRLAAMRMARAGREVTLVERGGLGGQCLHHGCMV
                     VCALADVARTVRYAGTLSELGVLDRPPRVSWPVLLAEMGEIQAIITRVLDKETEEAGV
                     RRLSGEGSIDGGTPFIDGKRVEAEAVLASTGSRPLIPRVVGVNLPGVVHAHTLTAWRD
                     LPRRIIIIGGGVMAAEFAHIFHAFGAEVTILARSSFLKGIDEPLRRQALKELAGVEIE
                     EGARLSGIKGDGHVRAVVVGKGRAEAELPADGVFLATGLTPNSEMISGPEKGPLGEVV
                     VDEYMQTSVPGVYAAGDLTGPPYLTPVARMEGLVAADNILGRERKMDYRCIPQSLTLM
                     NEFAWCSMENGPSVGTGSPSPAGPFSFWSVPEGNTGWSGIRANPEDGRILSATLAAPG
                     ASIVASYLAFLIRTGCTVQDFDEFIEVHPTTDGAFSLVRYLDEWMRNRKK"
     gene            complement(13864..15108)
                     /locus_tag="VKO45_09325"
     CDS             complement(13864..15108)
                     /locus_tag="VKO45_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06110.1"
                     /translation="MPQRTPGTSRRRRGRGRRGRVEAAPAPRGRREGEFPMNDWLARN
                     FPQFPPDRATILAATRHLSGPQRRNLFSPDPGTMKTAEGKWYEAIIYETILRCALASD
                     RISRVVSKGADARYRRLQARLGQNGLFYSRSGDIKVRGNGQDLAELDLLLVGEGRSIA
                     FGEIVTSPAAMKELEKEFLYKKQLMGHLFGQQVVPALLVSSVDISRISAVRRILEDPS
                     NLYLSTATCEELKRIVGREEVMRGTTRVARNPKLVEAPDLPLAGSFDYRRMHEVQRSR
                     VFSALANGTMYRGWDPREPQTVIVKKILLGALEPGAVNVFSNQVRLMYRGKPITPQEI
                     RTHFAKIILTVNLPEAEPIIYMKPRDRHYYLKMVRVRPGQFKFESNRSRKMLGFFLWL
                     EDIRPTLRPDIAGKLLNSGYFR"
     gene            15397..17448
                     /locus_tag="VKO45_09330"
     CDS             15397..17448
                     /locus_tag="VKO45_09330"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HMA06111.1"
                     /translation="MERSAYFSILAGEMGVAFRILGIITFLPLVVVLLTGEWRMFLPL
                     ASVPVVFLVLGRALAMSHPPSGEARLSIVLGAVAIIWFTCAAVGALPFILGGGMSVLD
                     SLFEAMSGWTCTGLTLIPVPETMPASLLFWRTFMQWLGGIGMVAFTITVLARSSLTNA
                     RCVRPPGDGAPFFPGLISTGREMWKIYVLLTLGSLVLILLTGVPLWDGLNLAMSAIST
                     GGFTVHAAGIAFYQSPLLEFALVPVMLAGSLPFMIYYLLYTRRRWTFFRDSQVRLILG
                     LVALGTVSIIIDLTYLTGEDSLTAFRHALFMSVSAITTTGFQDVPLQLWASVSVILLI
                     MLMAIGGASGSASGGIKAYRVLLGYHGIIWWLKRIFVSPKMVVPFEYQGQVIANDVAE
                     NEVRNNMLVIILFFLTVSVATVIVLHFSTIFFESTAVIFEIVSAVSNTGITTGFVSPE
                     MPSVAKWVFILVMWIGRLEIVPVLVLVLELANQSVGDDTAQISEVQARAGEGPAAPAV
                     GPSWSPGQEVPSEDAWKGEILAGTDVQPEVFEAAFQIAVAISREGREGKPVGTAFIVG
                     DAPAVLDRSRQLILNPFEGHAREERVITNPGIAENVKGFAILDGAFVVSGDGVIESAG
                     RYITIDTSTVQVPMGLGTRHGSVAGITRATNSIGVVVSQSGGRISIFRAGEMVKQFMT
                     L"
     gene            17537..>20192
                     /gene="uvrA"
                     /locus_tag="VKO45_09335"
     CDS             17537..>20192
                     /gene="uvrA"
                     /locus_tag="VKO45_09335"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HMA06112.1"
                     /translation="MPGKIVVRGAREHNLQDITVELPRDRLIVLTGLSGSGKSTLAFD
                     TIFAEGQRRYVESLSSYARQFLGLMSKPDVESIEGLSPAISIEQKSTSKNPRSTVGTT
                     TEIYDYLRLLFARIGVPYCPDHGIRIEAQTPERIAGRIAEEHPGTVTILSPVVRQKKG
                     TYQALIRDLHRDGFARVRVNGEIHRTDEDIPLERYKKQDIEIVIDRVSTDDRSRLAEA
                     CENALKRSEDGLVMVLDEEGKAHTYSSLMACPVCGIGFEELQPRMFSFNSPFGACEGC
                     NGLGIKMEFDPDLIIPDRDKSVAEGAVALYRNYIDGYRAQYLGAIARHFGFSVLTPIK
                     DLTKEQYNALMYGSSDRIRFSMSMSNGDGHWDHTGAWEGLLPQAERLYKQTQSEYRRH
                     ELEKFMQVSLCPVCGGKRLKKKVLAVKIAGKSIVDITDMPVAGVIAFFQGLPLTPKEK
                     EIAHQILKEINDRLRFLERVGLGYLTLSRAAGTLSGGEAQRIRLATQIGSNLTGVLYV
                     LDEPSIGLHQRDNRRLIETLKALRDLGNTLIVVEHDEETIRSADHVVDMGPGAGIHGG
                     AIVAEGTPDEIEGNPKSLTGLYLAGKMKIDVPKKRRRSKKFITLIGCRENNLKGIDVK
                     IPVGVFTAVTGVSGSGKSTLIYEILYRAMQRALHHSRVQVGSYDSIEFGDAIDKVIVI
                     DQSPIGRTPRSNPATYTKVFDDIRRVFAETKEAKVRGYSPGRFSFNLRGGRCEACQGE
                     GLIRIEMNFLPDVYIECEECKGTRYNRETLEVKYRGRSIADVLSMSVEEAMTLFEKIP
                     SIRSKLETLSRVGLDYIKLGQSSTTLSGGEAQRIKLTRELAKRATGKTLYLLDEPTTG
                     LHFDDTKKLIKVLDDLVEKGNTVIVIEHN"
BASE COUNT         3323 a         6743 c         6604 g         3522 t
ORIGIN      
        1 tatggatatc aaaaagaaac cggtcgatac aattgacgaa tacattgcac agtttcctcg
       61 tgaaatccag gagagattgc agaaattgag gaaaaccatc cgggaggagg caccggatgc
      121 cgaggagacc atcagctacc ggattccgac cttcaggctg aatggaaacc tggtgcattt
      181 tgccgcattc aaggatcacg tcagtttttt cccgacatcc tccggggtgg gagccttccg
      241 gcaggaattg tccggctaca agatctcgaa gggaaccatc cagttccccc tcgatgaacc
      301 gatgcccctc gacctggtca ggaaaatcgt gagattccgg gtccgggata acctggaaaa
      361 agggaagccc gggaaaaagc agtgacccgg cgatccagac gggaggcggg acaaacaccg
      421 caagctctat cttcattccc gggaagggtg tccccatgaa agcctcacac ttcctcctcg
      481 tcctcctcct tgccctcgcg gttcctgccg cctctgccct cgtaacgcag agcggcggga
      541 ccgtgaggat cgcccaggcg accgatgacg acgtctttgc gaccggcggg atggtcacca
      601 tcgatgcccc cgtcggaagc ctgatggccg ccgggggcca gatcatcgtg aacgcacccg
      661 tgaagggcga tgtcatcgcc gccggcgggc aggtcaccat caactccgat gtcggaggca
      721 aggttgtcgc cgccggtggc tcggtcaccg tgaacgggaa ggtcggcacg aatgcggtcc
      781 ttgcaggtgg gaccattgac ctcggaacca aggccaccat cgggagggac gccatggtgt
      841 cgggcgggtc cgtgaccaac gccgggaccg tcacagggaa gctctcggtg agggcgaaca
      901 cctttgagaa ccgggggaca gccgggaccc tggaggtgga gctccgtcaa cggtccaccc
      961 tgggcgggac cctcctctcg gtcttcggga tcctcttcac cattggcttg ttcatcctcg
     1021 gcctgatcct gatccggctc gcgccgggcc ggtaccatgc cgtcgaagat gagctcgccc
     1081 ggtcccccgt cgtgaaaacc gtggggggct ttatcgccct catcatcggg ttcatcgtca
     1141 tcctcctcct cgccatcacc ctcgtgggtc tccccatcgc catcctcctt ggcctcatct
     1201 acctcgccgg ggtcctcgtt tcgactctct tcgtctcctc cgtcctcggg aggagggtcg
     1261 ccctcctcct gaagcacccg gcctcggact atgtcgcctt caccatcggg ttcgtgatcc
     1321 tcgccgtcct gttccgcatc cccctcgtgg gaaccctcat cctcgtcgtt gcggtgagcc
     1381 tcggattcgg tgcggtcatg ttcacgctct ggaagaaccg ggcctgcatc tgcagtcctg
     1441 ccgctccccc cgccgggcag ccgtgaccgg caactcctct atttcttcgg ggatcgggcc
     1501 ttactccttc caccggtgct ggtacccccg ccgttccatg gggacgccgt tcaggaaggc
     1561 gccgggctca cccaccacat gcccgatgac atggtaggtc atcccttcga ggggaagttt
     1621 ctcggcgggg atggtgaaga ggagctcgta gtccccgccg ccatacaccg cgagttcgag
     1681 ggccttttcg tacgggatat cctcgacgac cgggatcatc gccgaggtga tccggtaccc
     1741 gcacgggttc gccagcgtga gatcgtacag cgagagggcg aggccgtccg agatgtccat
     1801 catcgaagtg gccccgctcc tcccgagtat ctggccctct ctgacccggg gctggggctc
     1861 gaagagggcc tttctgtact gggtatggcc atcgatggct gcctgggccc ggccgagcac
     1921 gccggtcacg cagacgaggt cccccacccg tgaacctttc cgccggacga ggtgttcggg
     1981 ctccacggtc ccgaggccgg tggagacgag ggtgagctcg tcgtggctgt cgatgtcccc
     2041 gccgacgagc tctgccccga accggtcgca gcagtccttt gctcccacca tgacgggccg
     2101 gaggcgctcc gcccggtcca ggcccacggc gaggaggatc caccgggggg cagcacccat
     2161 ggaggcgatg tccgagaggg tgaccgcaac cgccatccac ccgcactgcc agtcggtcat
     2221 tccctcgagg aaatccgtct tcgtgtggag catgtcggtc gtcgcgacga tgatccggtc
     2281 ccccaggggg aggacggcgc agtcgtccct cgcccggtcg ccgacgatgt cgagaatcga
     2341 ctccagcagg tcacggtcat ccacccttcc tcacctcctt ctcccgctct gtccggtact
     2401 ccctgaagag gtactccacc ttggcctttt ccctctcgat ccggtccttc tcccggtcct
     2461 cctcccagtt cgcgagggcc cggtcgacaa cgtcccgggc gaccgaaccc gcgggtcccc
     2521 gcatctggac ttccccggcc gggaccggga gaaccggtat ctcctcctcc cgcgaggtat
     2581 cgaggagaag ggggtcgggc cggcccgggg ggctcacccc gacgaggacc gctgtcaccc
     2641 ccaggccggc caggtctttt accaccgacc ggccccaccc gtcggatctc gcgacataga
     2701 ggaggtcccc ctccgcgatc cccgcctcct gcacgagggc ctggaccccg tcccgggtga
     2761 gggacgggag gatcttgaga gggagccggg acccctccac ccggacctcc tcggcctcct
     2821 tctcccgctc gaggcgcccc ctcagggact tcacctggcg ctcggcccgc cggagccggt
     2881 gcctcaggtc ccggatgacg gcgtcccttc gcgtgatctc ggcgtcccgc ttcatgcgga
     2941 gaaaggaccg cgaccgctcc cggtccaggc gttcgctgta cctggagatc tcggtctctc
     3001 tcgccttcac ctcctcctgg agctccgcga cgaggctccg gaggcgtttc accatcccgt
     3061 cgaggaccat gacccgctcg tcctgccttc ccgggacgac cggctcgggg ggtttttccc
     3121 cggcgggtgc ccgggccggg gagagctcgc ccaggatctg ctcgacggac tggccccgga
     3181 tgatgccggc cttcacccgg gccaggtcga aacccggcgg gacccgcttc aggatggact
     3241 ggaaccggtt cctgtacgag cggtaggcgt cgagggcggc cgagagggcg tccctttcgt
     3301 ggaggttccc gtacgagaag ccctcggtga gggcctgctt ctcctcgccg gtacggtcct
     3361 cgcggggggt gaagggcacc gccgagaagg ccctcctcac ccgctccacc gagaagggca
     3421 tcaccgagac atccgaggcc acgacaaggg gttttcccgc ctggtagagg gtctcgacga
     3481 tatcggccat ggacctctgc cggccgctgg agaggtggag gagattcccc tccaggtcca
     3541 ccgccgcgac cgccgtcgta gttcccgggt cgatccccac gatcaggtag cccgccccct
     3601 tccccgccgg tttgaaccgg atccggtcga gcctctggcc ggagacccgc acctggacat
     3661 cccccccgcg ccgggaggag accgggatct cctcgcgggg tgcatgaacg aggaactgga
     3721 cccgggatag ccccccgaag gcccgggact ccctcttctc gtaccggagg cccgcctgga
     3781 cgaggcgctc ctcgatctcc cgggcctccc ggagcacggc gccgtggacc ttccggacat
     3841 agcggttctg gctccaccct cccttcccga gggaccggtt ccgggagacc acgaccaggc
     3901 agctgttctc ccaggcgacc acctcggcac cgcctcccag ggaggcgatc ttcgccgccg
     3961 cccgggcctc ggcaaagggg tcgaaccggt caaagctgat gttgtaccgg gccgcgacct
     4021 tcccgagggt ctcggggcgt tcgccgcccg tcacctggat gagccgggtc ctcggcggga
     4081 gatgctcgag gaaggcgaag agctcgtgct ggtcccgggc gatctcctgg agactgtcga
     4141 cggcaaggat gtcaggttcc tcggcggaga tctcccggag gagccggaac tgggtcacct
     4201 ctccctcccc ggtgacgtcg ccgtcctcca tccggacgag ggcgtagacg ggccggcggg
     4261 tccgggaccg gaccgatccc ttcacgacgt cgataccaaa caccttcacc cggtcacctc
     4321 cccgaacgcc cgggagaggg ggtactcgat cccgtacttc ctctcaaagt agagcaggat
     4381 aacccggagg tccctctcca ggatctcccc tgcattcggg tgggtcgcct ccatccactg
     4441 gggccagtcg atgaggaagc accgctcccc ctcgatcatg acattgaact ccgagaggtc
     4501 ggcgtggacc acgccgagcc ggtacgcctc cctcacctgg acgaggatgg catcgagcat
     4561 ggcccgggga tgctcgaggc cgcagcggga gaggatctcc cccggtatca gggacatcac
     4621 gaccacgtgg cggttccgct cgatggggac ggggaccgag accttcgggt ggagcttcgt
     4681 gagcgccagg aactcccgct cggccgagaa ggcggaggca aagagccagg ggacatgccc
     4741 cgcctcgggc atataccccc gtttcaacct tgcagactgg aaggatctct gcccgattcg
     4801 gtggaacttg aggaccagcc ggccgaggcc gagcccctca tagacgatgg actccttccc
     4861 ctccccgatg aagggaccga gggccgatac cgtacccttc ccggacaggg catggagggc
     4921 gagggcgtcg taccccgtga agacgagcga gtagccctcg tagggcacct tctcatactt
     4981 cgccatgccg aactgcagga gccgggtgag gcggtaggag acctccttct cccccttccc
     5041 cgtggcatgg gcaagcacat ccagggggac ccactggaac cgcttcatga gccgctcaag
     5101 ggtatggagg accaggaggt cgttctcctg gagatttcga accgcctcgg ccgagatggg
     5161 catggcgctc tacacacttt atgtgagggg atgataaaag tctgatcagg gtgccccgat
     5221 gcggtgcgac cggtgcgacc gggatgccat cctcctccag cggtactcgg ggctccgtct
     5281 ctgcggggag catctcatct ctggcctcga atccagggcg aaggggacca tccgggccca
     5341 cgggtggatc cggcccggcg accggatcgc cgttgccctc tcgggcggcc ctgccagcgt
     5401 ctccctcctc cacttcctcg tgacgcactt cgggatgcgc cgggatctct ccttcatcgc
     5461 catcaccgtg gatgagggag gctggacgga cctggggagg acccgggcca ttgccgaggg
     5521 aatggggatc gaatgggccg ccgcctcacc tcaccgggag atccgtgggg atcgtgtccc
     5581 cgggcaaacg gaggctcctt tcgggggaac agggctccgc gacgatgccc tcgcatccct
     5641 cgcgagggac ggtggtgcga cgaaactcgc cctgggaacg agcctcgatt cgatggcccg
     5701 atcggtgttc ctgaaggtcc tcgacggcga cggagcgggg ctgacccggg gttcgggtga
     5761 aggagagggc gggatcccga gaatcgagcc gttcatccgg atccctgccg gggagctcgc
     5821 cctctacgcc atgctcaatt ccctcgacca tgtttcggga cggggcatgg atgcccggga
     5881 ctccctggag gccgaggcag gccggctcct ggatgacttc gccgcccgcc atccgtcagc
     5941 cccgttctct ctcgcgaacc tcggcgaggc cctctctggc ggaacgggcg gagggaggac
     6001 ccatggctga agggatcccc ccctggaggt tcatcttcgg gcagggccgg agggggattc
     6061 ccctccttgc cgggatcttc atcctcgccc tcatcttctt cacggccatc ttccatacca
     6121 tctatcccat attcgagggg agggagatct catgggccca ggccctcctc ttcatcctcg
     6181 agaccgtctc cactaccggg tatggcgacc tcctcccctt ccggagcgat tatacgatgc
     6241 tcttcgccat cgtcctgatc ctttccggcg tcttcatgat cttcatgttt atcccggtcc
     6301 ttctcgagcc gatgctcacc cgcatcatca gcgcccctcc ccccaggggc acgaaacggg
     6361 agatgaaggg ccacgtggtc atcgccggct acggcccgct ggcccgggcc ctcatcgaca
     6421 gcctcatcat ctcggacctg gggatcgtcg tggtggaggc cgacgagagg gtggcacggg
     6481 aggtggtctc ccactacccg tcccgggtct cggtaatctg gggcgactac cgggcccccc
     6541 gcacctggga gcaggcctgg gtgcggcacg cccactccgt cgtggtctgc gaggacgagc
     6601 ggaccgcggc ggagatcatc ctcggcgtcc gggacctgac ggcggggtcc atcatcgcgg
     6661 tggtggatga cctcgcctat gaccggtacc tccggtacgc cggggccgag tacgttctct
     6721 cgccgaagaa ctccaccggc aagatcctgg cccgccacgc cgtgatccgc cccgacgtag
     6781 acaccatctt cgaggccatc agccttgacc acatgaggct cgaggagggg agatcggccg
     6841 acggaagcct caagctcgtc aaggtgccgg tcatgcccgg gtgcatcgcc ttcgggaaga
     6901 gcctggggga gctctccctc ttcgagcggt acggggtgga tctcctcttc ctgtggaagg
     6961 cgggggagtt cgtcccttcg ccgcaaaggg aggatctcct ggacgcctcc accatgctct
     7021 tcctcctcgg gaaggcctcc tcggtctccg aagtcctgga ccgcgagttc tgcatccgcg
     7081 agacagggga gccctcggcg gtcatctcgg gatacgggga cgtggggaag gccgcctacg
     7141 cggagctgac cggggccggg gtccggtgca ccgtcatcga ccggaggagc cacggcgtga
     7201 gcgaggtggt gggcagcgcc gaggacgagg ccgtcctccg ggccgccggt gtcggggagg
     7261 cccggttcgt catcgccgcg gtcaacgacg acagcgtgaa catcttcacc accctcgtcg
     7321 cccggaacct gaacccctcg gcccggatcc tcgcccgggc gagcgagccc gcctcggtgg
     7381 agaagctcta caaggccggg gcggattacg tcgcccttct cccgaccatc ggcggccagg
     7441 tcatcgccgg gatcatcctc tcggacatcg tccgggtcct cgtcgatctc ccgaacgggc
     7501 agaaggtgct catgaagcac ctcacccggc atgccggggc gacggtcggg ttcctggaga
     7561 gaaggaccgg agtcaggatc gtgggcctcg aggggcccgg gcgttcggtg gtgagaccgg
     7621 ggcccgggga accggtgggg gatggcgact cgatcatcgc ggtggggggg aaccgcgagc
     7681 tgaagaggct catccactat atctgaagaa tcatggctcc ctgctcccga tcgggagccg
     7741 ggagctctag gagaagatct ccggatactc ctcgggccgg ttcaggaacc cgaggctttt
     7801 cgtgccgacg agatcgtaga gggggcgccg gggaaggaaa tccaggccct tctggtcccg
     7861 ggccgaaagg atcctcgccc cggccgcgtg ggggtagcgg gggttccgca cgagggcacc
     7921 cccctcgagc tcgaaccagg ccccgccccc gaatttctcg tagaccgagt aatcgctctc
     7981 gaaccgggtg gagacgatgt ttgccatgga gagggtggac ctcccggggt tgatggttac
     8041 atgcccgtac ccaggtggga cgaggacacg gtcacccggg cctgcccgga ccaggaggac
     8101 gtcctccgcc gacctcgtct ggaggaggta gtgggcctca ccctccagga tctcgtagat
     8161 ctcgggatag gggacaccct tcccgttcgc cgggtggtgg tgccccttgg tcttcacgca
     8221 ctccccgcag agggtcctcg cgggaatgac cgtgaggtcg taccggaggt cctgctcacg
     8281 aagccaccgc cggtcctccg gggatttcga gaggtcgcgg tacatcgagt agagaggctc
     8341 tgcgcagccg caggccgggt ccatgaggac ccccctgatc tcctcgatgg tgcggaggcg
     8401 gggggccggg agggggccgt cccagggaat catcacttct tcttgcgctg gatgaagata
     8461 taaacgagga cggccccgat gagaacgacg aggacggcga ggaccagggg gtttccgagg
     8521 atcccgagga tcccctgaga cgggaccacc tccacctgga ccttgatggt gtcagagatc
     8581 tggctgttgt ccagggcgtc ccggtaccgg atctcggagt cgaggccgta gctcttcggg
     8641 gtcgcgcccg aggccaccgt gacgtcgtag agggcggtcc cgctctcccc cggtgcgagg
     8701 tccccgaggt aggcggtgtc atcgctcgag gtgaagggat cgacaaggga gatccgggcc
     8761 tcggcggcgt ggaccgccgc ggagccggtg ttccggtagg tcacctcgag ggtcacggtc
     8821 tgccccgggt tcacctgggg gacgggggag accacggcga agtcgatctt gctccccacc
     8881 ggaacaccaa tggtcacggt gtccgagctc gccgattcgc cctccgagtc ggtgtactcc
     8941 acgtggacat cgacaggata gctctgcgcc tcggcatccg cagagaccga tgcccggaac
     9001 ctgatctccc gggtgtcccc cggcccgaag tccccgaggt agaccgaggc atcggtgggg
     9061 atgaggggcg agagcccgtc ccgggtgagc ttggcgaccg cgtcccgggc ggtctcgctc
     9121 ccggcgttcg tgatggtgac gacgatgtac ccctcggttc cgacattcag ggtgtctgcc
     9181 cttgacatca ccggatcgag ggtcactccg ggcttgatcc tgatgtcgag gggaatcgtg
     9241 atgttcgtgg tgtagtacca gtacctgagc gtgtcaaagc tcaccttctc ggcggtattg
     9301 aggtactggt agttgagctg gagggggagg gagtacaccc cgcccttcgc gtacttcgag
     9361 atcttcaccg agaaggtggc cgcgacgctg ctcccgccgg taagatcccc gaccatctgg
     9421 ggatcggtct tcaccgtgac gggtgccgtc ccggacgaga ggcccacggt caggagcttc
     9481 gccgtgttcg gtttgtcgat cggggtgatg atggtagact gggtgatggt cacctcgttc
     9541 accccggtgt tctggacctt cacggtgagg gttacgtcct cgccgggggt gaactcgttc
     9601 gtccccgaga tggccgcgga gagctggggg gatccgaaga gctgctcttc accggccagg
     9661 gccggtgccg cgaggagggc gaggagggcg atgatagcga taagcggggg aagtgagcga
     9721 ccttgcatca ggattcacct gttgtcttga taataacaac atttatctgg cttcggtatt
     9781 tatacttact tccatggaga tgggagagga cctcagggcc gcccttccgc agatcctgaa
     9841 atccctcggt tttaccaagt acgaggccct tgtctacgtt gccctcctgg gaatggaggg
     9901 cgcgaccgcc acggagatcc acgagttctc ccgggtcccg cgcgcatccg tgtacccggt
     9961 cctcgaccgg ctcgtggaga agaacctggt caccgtttcc catgccaccc cgaaacgctt
    10021 ctccgcgatc cccccggagg aggggatcgc aaacctcctc tcccgcatcg aggaggatgc
    10081 cctccgcgca cgggagatcc tcggggatat ctaccggaag cgggtgacgg tggagagggg
    10141 cgaccaggag ctcatctggt ccatctacgg ggaggagaag atcgccacgc gactcaggga
    10201 ccttatccgg ggggcgaaga ggagggtgca gctcttcacc acctggcctt tcctcgagag
    10261 ggtcctcctc cccgagctgg tccagctctc tcctcccgtc gaggtggagg tggtcacggg
    10321 ccactgggag gggcctgttc ccccggggat gaaggtccat gtgaggcccc tgcggggaca
    10381 tcccgaacgc gttcccatgg agacggccgg gatctttatc atcgatggaa agagggcgat
    10441 ggtcatcatg ggtgtcaggg aggaggcccc cacggccatc ttctcggagt ccccgggctt
    10501 cctccggttc ttctcacaga tcctggccat gaacctcgcc tgggcctccg gcccggatca
    10561 gtagtcccgg atggagccca tttcccggac atccatgacg cctttcaggg ccctcgcttt
    10621 ggcgacacag tagccggatc agtagtcgcg gatcgacccc atctcccgga cgtccatgac
    10681 ccccttcagg gccttcgtca ccaccgggat cccggcctcc ttcagtgccg ccatggggtt
    10741 cgtgccgccg accatcgcga ccccgaagta ctgggggctg accgggaccg cgaggatggg
    10801 gacgttgggc gcccccacct cgaggatgcc cgtgaatccc acctcggaga ggtcgtccag
    10861 gagccccatc accagggact cggcctccat gtggcactcc cgcaggttcg cgaggatggt
    10921 gccgctcccc tccttcatga cccgggtgat ggaggtgagg tcctgggcga cgagcacctc
    10981 gagcgggtcg atggtggtcg cctcgtagag gatcatgtgg gtgaaccggc gcggtacccg
    11041 gtcgacgatc tcgacgagcc cccctcccac cggctggatg gggacccccc gcctgaggag
    11101 gataccgtcc agggtgatgg tgcagagggt gcagatcccg ttcaacccct cagggatggt
    11161 gagtccctcc agcttcgtgc cggcatcgag gatccggacc cggtcgctca ccgagaggcc
    11221 tgcccggtag gcgttccgga aaagcgtgat ggccacctcg agcctgtcgt cgggtatcag
    11281 ggacaggttg aagatgatct tccccgtgtt ctccaccggg ttgtaggtca cctccatggc
    11341 gaggtcctcg atccggttgc tgacaaaggc gaggctcggg caacagggtt cgcagggatt
    11401 gacgggattc tccggttcca tgggcacctg taccggtaca ttttccccgg ttctctgata
    11461 taggtgtcga accggcctcc gggggcccgg tgcactctgt ccgtcgtcgg ttcccgcccg
    11521 gggagaagaa atgttctata gtagggaggc gatattttgg tgaatggaca gcgagatgag
    11581 agatcgggcc cgggaggcgg tgcggcggat cctggaggcg gcctcgtacg aggtggagga
    11641 cctggagcgg cccctcgacc tctcggccat cggtgacggc gagtgcgtca tcgtccagtg
    11701 ctcggataac acgggggaga tcgcccagtt cgaccggacg aactaccggt gccgggtcga
    11761 ggaaaaggag gtcacgagcc ggaagctcct cttcaccctg aacgactcgg cggtcaccgg
    11821 gcattgtgtc cggtggggaa tccgggagct cggggagttc gccggccgtg cggccatggc
    11881 gacggtcctc ggggagaggc tcagcctcga cctcggggcg ggtgcggccg aggcctccac
    11941 gccggccata gaccaggcgg ggccggagct cccccatatc ccggtgaagg tggacgaaaa
    12001 gcgggcccgg gccatctccg gggagagcgg ggccgggacg ctgcgcttca tcccctactg
    12061 gcggtaccac taccggagca cggggcagaa gggctacggg aaccaggtgg tcaccttcaa
    12121 cgccgagaag aagggggcca tcaatgccat caacggcgcg gagaccggcg aggagctcgg
    12181 gcaggtccag gtctcgccgg tccccgtctc ggcggtggtg ctccagccca tggtccagaa
    12241 gtccgaggcc gaggagaagc tccgggaccg ggtcgtcgag gagctcaccc agaaggtgcg
    12301 gatcaagcag gtcaaggggg acaccatctt ctacgaagag cggatcttca agccggacag
    12361 gaaggacatc gccatcgagc tcgagctcgt ccacctgccg gtctggcagt tccgggggaa
    12421 gaagttcctc gagctgaacg cggtcacggg cgtcgtcctc accgagccga tggacacggg
    12481 cgccgagctc ctctgaacgg gccatgatcc tcgtcatcgg cggcgggccg gccgggcgcc
    12541 tcgcggcgat gcgcatggcc cgggccgggc gggaggtgac cctcgtcgag cgggggggcc
    12601 tcggcgggca gtgcctccac cacgggtgca tggtggtctg cgccctcgcc gacgtggccc
    12661 gcaccgtccg gtacgccggg accctgtcag aactcggcgt cctggaccgc ccgccccggg
    12721 tctcctggcc ggtgctcctc gcggagatgg gggagatcca ggccatcatc acccgcgtcc
    12781 ttgacaagga gaccgaggag gccggggtga gacgcctttc cggtgagggg agcattgacg
    12841 gaggtacccc cttcattgat ggcaaacggg tcgaggctga ggcggtcctc gcctcgacag
    12901 ggtcccgccc gctgatcccc cgggttgtgg gggtgaatct cccgggcgtg gtgcatgccc
    12961 atactcttac ggcgtggagg gatctccccc ggcggatcat catcatcggc gggggggtga
    13021 tggcggcgga gtttgcccac atcttccacg ccttcggggc ggaggtcacg attctcgcga
    13081 ggagcagttt cctgaaggga atcgatgaac ccctcagacg gcaggcactg aaggagctcg
    13141 ccggggtgga gatagaggag ggtgccaggc tctccgggat aaagggggac gggcatgtca
    13201 gggccgtggt ggtaggaaag gggagggcgg aggcggagct ccccgcggac ggggtcttcc
    13261 ttgccaccgg gcttaccccg aactcggaga tgatctccgg ccccgagaaa gggccgctgg
    13321 gcgaggtggt ggtggatgag tacatgcaga cgagtgtccc cggggtgtat gccgccgggg
    13381 atctcaccgg cccgccctac ctcacacctg tcgcgaggat ggaggggctc gtcgccgcgg
    13441 acaacatcct cgggagggag cggaagatgg actaccgatg catcccccag tccctcaccc
    13501 tcatgaacga gttcgcctgg tgctcgatgg agaacgggcc ttcggtcgga acgggatctc
    13561 cctcgccggc cggccccttc tccttctggt cggtacccga agggaacacc gggtggtcgg
    13621 gaatcagggc gaacccggag gacgggagga tcctctctgc caccctcgcg gccccgggcg
    13681 cctccatcgt cgcctcgtac ctcgccttcc tcatccgcac gggttgcacc gtgcaggact
    13741 tcgacgagtt catcgaggtc catcccacca ccgacggggc cttctccctg gtccgctacc
    13801 tcgacgaatg gatgaggaac cggaaaaagt gaagggtacg ggaaccccgg cacggcgggg
    13861 attttaccgg aagtatcccg aattgaggag ctttccggcg atatccggcc gcagggtggg
    13921 cctgatgtcc tcgagccaga ggaaaaaccc gagcatcttc cgggaccggt tactctcgaa
    13981 cttgaactgg cccggccgga ccctgaccat cttcaggtaa tagtgccggt cccggggctt
    14041 catgtagatg atgggctctg cctcggggag gttcacggtg aggatgatct tggcaaaatg
    14101 ggttcggatc tcctgcgggg tgataggctt cccccggtac atgagccgca cctggtttga
    14161 gaagacgttc accgcccccg gctcgagggc tccgaggagg atcttcttca cgatgaccgt
    14221 ctggggttcc ctcgggtccc agccccggta catggtcccg ttcgcgaggg ccgagaagac
    14281 ccggctccgc tggacctcgt gcatccgccg gtagtcgaag ctcccggcga gggggagatc
    14341 cggagcctcg acgagcttgg ggttccgggc gacccgggtg gtgccccgca ttacctcctc
    14401 ccggccgacg atccgtttca gctcctcgca ggtcgccgtg gagaggtaga ggttcgaggg
    14461 gtcctcgagg atgcgccgga ccgccgagat gcgggagatg tccaccgagg agacgaggag
    14521 ggcggggacc acctgctggc cgaagaggtg gcccatgagc tgcttcttgt acaggaactc
    14581 cttctcgagc tccttcattg ccgccgggga ggtaacgatc tcgccgaagg cgatggacct
    14641 cccctcgccc acgaggagga ggtcgagctc cgcgaggtcc tgcccgttcc cccggacctt
    14701 gatgtccccc gaccgggagt agaagaggcc gttctggccg agccgggcct ggagacgccg
    14761 gtaccgggcg tcggcaccct tggagaccac ccgggagatc cggtccgagg cgagggcgca
    14821 ccggaggatg gtctcgtaga tgatggcctc gtaccatttc ccctccgcgg tcttcatggt
    14881 ccccgggtcg ggggagaaga ggttcctccg ctgggggccg gagaggtgcc tcgtggccgc
    14941 gaggatcgtg gcacggtcgg gggggaactg ggggaagttc cgggccagcc agtcgttcat
    15001 ggggaactcc ccctccctcc tgccccgggg tgccggggcg gcctcaaccc tgccccttcg
    15061 tccccgtccc ctccggcgcc ggctcgtccc gggcgtgcgc tggggcatac tcctcccggc
    15121 gggccctccc cccggtccgg gagaggctgc caccggggga tcccgcggca tgcgggcctc
    15181 cccttccgct gatccccgag attcgaccct atgcacacta tcatctctcc cgagatgcat
    15241 cataatcctt taccccggtg cccttagtac cgcacccccg gtgcccttag taccgcaccc
    15301 ccggtgcccc tggaaaccgc acccagggcc gcctgccgca gaccgtacct tccatgaaga
    15361 aacattgtaa tagggaggcg acccacgttt ctaccgatgg agagatctgc ctacttctcc
    15421 attctcgcgg gggagatggg ggtcgctttc cggatcctcg ggatcatcac cttcctaccc
    15481 ctggtggtcg tcctcctcac cggcgagtgg aggatgttcc tccccctggc gagcgtgccc
    15541 gtggtcttcc tcgtcctcgg ccgggccctc gccatgagcc atcccccctc gggcgaggcc
    15601 cggctctcca tcgtgctcgg ggccgtggcc atcatctggt tcacctgcgc ggccgtggga
    15661 gccctcccct tcatcctcgg ggggggcatg tccgttctcg acagcctctt cgaggcgatg
    15721 tccgggtgga cctgcaccgg cctcaccctc atcccggtcc cggagaccat gcccgcctcc
    15781 ctcctcttct ggcggacctt catgcagtgg ctcgggggga tcgggatggt cgccttcacc
    15841 atcacggtcc tcgcccggtc gagcctcacg aacgcccggt gcgtgcgtcc ccccggcgat
    15901 ggcgcaccct tcttcccggg cctcatctcg accgggcggg agatgtggaa gatctacgtc
    15961 cttctcaccc tgggttccct ggtcctgatc ctcctcaccg gggtccccct ctgggacggg
    16021 ctgaacctcg ccatgagcgc catctccacc ggcgggttca ccgtccatgc cgcggggatc
    16081 gccttctacc agagccccct cctcgagttt gccctcgtcc ccgtgatgct cgccggttcc
    16141 ctccccttca tgatctacta cctcctctat acccgccggc gctggacctt cttccgggac
    16201 agccaggtcc ggctcatcct cgggctcgtc gccctgggga cggtctccat catcatcgat
    16261 ctcacctacc tcaccggcga ggactccctg accgccttcc ggcacgccct cttcatgtcg
    16321 gtctcggcca tcacgaccac cggcttccag gacgttcccc tccagctctg ggcctcggtc
    16381 tcggtgatcc tcctcatcat gctcatggcc atcggggggg cctcgggctc ggcgagcggg
    16441 gggatcaagg cctaccgggt gctcctcggg taccacggca tcatctggtg gctgaagcgg
    16501 atcttcgtct ccccgaagat ggtcgtcccc ttcgagtacc aggggcaggt catcgcaaac
    16561 gacgtggccg agaacgaggt gaggaacaac atgctcgtca tcatcctctt cttcctcacc
    16621 gtctccgtcg cgaccgtgat cgtcctccac ttctccacca tcttcttcga gtcgacggcg
    16681 gtcatcttcg agatcgtctc cgccgtgagt aacaccggga tcaccacggg cttcgtctcg
    16741 cccgagatgc cctccgtcgc gaagtgggtc ttcatcctcg tcatgtggat cggcaggctg
    16801 gagatcgtcc cggtcctcgt cctcgtcctc gagctcgcca accagtccgt cggggacgac
    16861 acggcccaga tctccgaggt ccaggcacgg gcgggggagg ggccggccgc acccgcggtg
    16921 ggcccgtcct ggtcccccgg gcaggaggtc ccctccgagg acgcctggaa gggggagatc
    16981 ctcgccggga cggacgtcca gcccgaggtc ttcgaggcgg ccttccagat cgctgtcgcg
    17041 atctcccggg aggggcggga ggggaagccg gtcgggacgg ccttcatcgt cggggatgcc
    17101 ccggcggtcc tggaccggtc ccggcagctc atcctgaacc ccttcgaggg ccacgcccgg
    17161 gaggagcggg tcatcacgaa ccccgggatc gccgagaacg tgaagggctt cgccatcctc
    17221 gacggggcct tcgtggtctc gggcgacggg gtcatcgagt cggcgggccg gtacatcacc
    17281 atcgacacct cgacggtcca ggtccccatg ggactcggga cccggcacgg ctcggtggcc
    17341 gggatcaccc gggccacgaa ctccatcggg gtcgtcgtct cccagagcgg cgggaggatc
    17401 tccatcttcc gggccgggga gatggtgaag cagttcatga ccctctagag ggttccttgc
    17461 gaaagggtgg ttcctcactt tttttccggt ggcgaagccc ctatatagct ccggagggaa
    17521 tggatctatc cacaaaatgc cgggaaagat cgtcgtaagg ggtgcgcggg agcacaacct
    17581 gcaggacatc accgtggagc tcccgcggga ccggctcatc gtcctcaccg gcctctcggg
    17641 ctcgggcaag tccaccctcg cctttgacac catcttcgcc gagggtcagc gccggtacgt
    17701 cgagtccctc tcctcctatg cccggcagtt cctcgggctc atgtccaagc ccgatgtcga
    17761 gtccatcgag ggcctctccc cggccatctc catcgagcag aagtcgacct ccaagaaccc
    17821 ccgctccacc gtcgggacaa ccaccgagat ctacgactac ctgcggctcc tcttcgcccg
    17881 gataggggtc ccctactgcc ccgatcacgg gatccggatc gaggcccaga ccccggagcg
    17941 catcgccggc cggatcgcgg aggagcaccc ggggacggtg accatcctct ctcccgtcgt
    18001 gcgacagaag aaggggacct accaggccct catccgggat ctgcacaggg atggcttcgc
    18061 ccgggttcgg gtgaacggcg agatccaccg gaccgacgaa gacatccccc tggaacggta
    18121 caaaaagcag gacattgaga tcgtcatcga ccgggtctcc accgatgacc ggtcccggct
    18181 tgccgaggcc tgcgaaaacg ctttgaagag atcggaggac gggctcgtga tggtcctcga
    18241 cgaggagggg aaggcccaca cctactcctc cctcatggcc tgcccggtct gcggcatcgg
    18301 cttcgaggaa ctccagcccc ggatgttctc cttcaacagc cccttcgggg cctgcgaggg
    18361 ctgtaacggc ctcgggatca agatggagtt tgaccccgac ctcatcatcc cggaccggga
    18421 caagtccgtc gccgaggggg cggtcgccct ctaccggaac tacatcgacg ggtaccgggc
    18481 ccagtacctc ggggccatcg cccggcactt cggcttttcc gtcctcactc cgataaagga
    18541 tctcacgaag gagcagtaca acgccctcat gtacggctcc tccgaccgga tccggttctc
    18601 catgtccatg agcaacgggg acggccactg ggaccacacc ggggcgtggg aggggctcct
    18661 cccccaggcc gagcggttgt acaagcagac ccagtcggag taccgccggc acgagctcga
    18721 gaagttcatg caggtctccc tctgcccggt ctgcgggggg aagcggctca agaagaaggt
    18781 cctcgcggtg aagatcgccg ggaagtccat cgtcgatatc accgacatgc cggtggccgg
    18841 cgtcatcgcc tttttccagg gcctccccct caccccgaag gagaaggaga tcgcccacca
    18901 gatcctgaag gagatcaacg accggctccg gttcctcgag cgggtgggcc tcgggtacct
    18961 caccctctcc cgggcggcgg gaacgctctc cgggggagag gcccagcgga tccgcctggc
    19021 gacccagatc ggctcgaacc tgaccggcgt cctctatgtc ctggacgagc cctccatcgg
    19081 cctccaccag cgggacaacc gccggctcat cgagacgctg aaggccctgc gcgacctggg
    19141 gaacaccctc atcgtcgtgg agcatgacga ggagaccatc cggtccgccg accatgtcgt
    19201 ggacatggga ccgggggcgg ggatccacgg cggggccatc gtcgccgagg gaacacctga
    19261 tgagatcgag ggaaacccca agtccctcac aggcctctac ctcgccggga agatgaagat
    19321 cgatgtgccg aagaagcggc gcaggtcaaa gaagttcatc accctcatag gctgccggga
    19381 gaacaacctg aagggcatcg atgtgaagat ccccgtcggg gtcttcaccg ccgtgaccgg
    19441 ggtctcgggc tcggggaagt cgacgctgat ctacgagatc ctgtaccggg ccatgcagcg
    19501 ggccctccac cactcccggg tccaggtggg atcgtacgac tccatcgagt tcggggacgc
    19561 catcgacaag gtcatcgtca tcgaccagtc ccccatcggc cggacccccc ggtcgaaccc
    19621 ggccacgtac acgaaggtct ttgacgacat ccggagagtc tttgccgaga cgaaggaggc
    19681 gaaggtccgg gggtacagcc cgggccggtt ctccttcaac ctccggggcg gccggtgcga
    19741 ggcctgccag ggcgaggggc tcatccggat cgagatgaac ttcctccctg acgtgtacat
    19801 cgagtgcgag gagtgcaagg ggacccggta caaccgcgag accctggagg taaagtaccg
    19861 ggggaggtcc atcgccgatg ttctttctat gtccgtggag gaggccatga ccctcttcga
    19921 gaagatcccc tccatccgga gtaagctcga gaccctctcc cgggtcggcc tcgactacat
    19981 caagctcggc cagtcctcga ccaccctatc gggtggcgag gcacagcgga tcaagctcac
    20041 ccgggaactc gcgaagcgtg cgaccggaaa gaccctctac ctcctggacg agccgacgac
    20101 cggcctccat ttcgacgata ccaagaagct gatcaaggtt ctcgatgacc tcgtggagaa
    20161 gggcaacacg gtgatcgtta tcgaacacaa cc
//
LOCUS       DATGDI010000248         6028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_231209, whole genome shotgun sequence.
ACCESSION   DATGDI010000248 DATGDI010000000
VERSION     DATGDI010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231209"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VKO45_09340"
     CDS             <1..990
                     /locus_tag="VKO45_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F430 synthase"
                     /protein_id="HMA06113.1"
                     /translation="PERPLPPWTATVPLDPGLPAGPGILSRGAPTPPYAPGSFGPGIL
                     HTSRGTFRYPGQTLLWRRSITPASVVPAAKEAVREGGWLVAEESLGVSGAGDAAVLTS
                     GDDYRIAAGKRSALAAKRESMREVPLVVVPPGARFDHPNLVDCGECVSWDGDLCSYHH
                     NGIRGEFRNPLGDLPPYRVAISLAAATLCALGRDPSALASFGGVEGRLSVERKDGVIV
                     VDNANSGATAGTTEAAASYARAVSGGGWITLVIGEEDRAVCEGFPPRDIDSSIQKIHP
                     DRVILVGEAARAVRPGGFGRRIAYADTLDAARALALRETPAGGAVVLSVKTWR"
     gene            993..2057
                     /gene="cfbD"
                     /locus_tag="VKO45_09345"
     CDS             993..2057
                     /gene="cfbD"
                     /locus_tag="VKO45_09345"
                     /EC_number="6.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase catalytic subunit"
                     /protein_id="HMA06114.1"
                     /translation="MDYIQPRPSSIVAALYTARDLDVDVAILHGPSGCSFKHARLLEE
                     DGMRVLTTSLADHEFIFGGQGPLERVLRYAEQRFSPARMAVIGTCVSMIIGEDLPSAL
                     AGSGVSTPTIVVNIHAGFRENIEGVIATLEAAAEAGWIGTAELERQRYLLARANEVEH
                     LRGAAIQEYIEPSRGDLKHVAAARLLEMARSGEHGMAVLNAKKETAYMFADELLALHE
                     VCPDAPITYLANLEERGLPKVREDARIIRAGMVERGLTPGLLGALDEYGANGDAIGER
                     IAAEAPAFLLLAGVPHAVPPEYTKGIEVFSVTNGPRQVEPLRLLGHQHVVVEVDLHPK
                     TLGVKHIVESEFGAVLRSLR"
     gene            2054..3427
                     /gene="cfbB"
                     /locus_tag="VKO45_09350"
     CDS             2054..3427
                     /gene="cfbB"
                     /locus_tag="VKO45_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide synthase"
                     /protein_id="HMA06115.1"
                     /translation="MKALLISGDRSGTGKTSITLALASLFSERTPVQTFKAGMDYIDP
                     SYLSAVTGRPCRNLDGFVMGDREIQASFSHGCLGAGLALIEGVRGLYEGADALTDRGS
                     TAGIAKDLGVPVILVVNARSITRSAAALVKGFQAFDPAISIRGVILNSVGSESHTRKA
                     VTAIEHACGIPVLGAIPQDSRVELTMRHLGLVPYREGSGDGEFTNRLAAVRELVSAHV
                     DLDRVLACAADLDPVSSDPSPFTAEKKPDVRIGVAYDEAFNFYYADLFDLLPALGAEV
                     VTFSPVQGRLPEADGYILGGGYPELFGPGLEANEGMKEGLRQASLDGKPIYGECGGLM
                     YLTDRIVLAKGWRGGPGASYAMCGVFPGETRMPASRVVSYVEGVASGESPLGPGPFRG
                     HEFHYSDVVLDSGTRYAYRITRGLGIRDGLDGALVRRTLGSYTHLHPLASLDMFRAFV
                     EGCRGKR"
     gene            3785..4648
                     /gene="ilvE"
                     /locus_tag="VKO45_09355"
     CDS             3785..4648
                     /gene="ilvE"
                     /locus_tag="VKO45_09355"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HMA06116.1"
                     /translation="MWIYLDGRFVQKEEAKVSVFDHGLLYGDGVFEGIRAYNGKVFRL
                     DGHVDRLYDSAKTINLIPPLSKDEMKALILETMRRNKLRDGYIRPVITRGVGDLGLDP
                     RKCPRPTVIIIAVEWGAMYGDLYEKGLKAVSVSVRRNAAESLPPNAKTLNYLNNILAK
                     MESYLKGGDEAIIFDTNGYLSEGSGDNIFVVKNGVLATPPCLNNLRGITQQVVVECAK
                     ELGIEVWWQNMGFFDLYTADEVFVTGTAAEVAPIVEIDGRSIGEGRPGPVTKKLMKAF
                     REVTKREGTPI"
     gene            4713..5096
                     /locus_tag="VKO45_09360"
     CDS             4713..5096
                     /locus_tag="VKO45_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06117.1"
                     /translation="MEKNLKIILSGVIAWVIPFIVSVPLYPHGQPISDIQVTKSILIV
                     VGALVGAVLALWYLRDVRQDFTREGAILGIAWFVINSALDILVLVYLLHGMDLASWTG
                     QIGIRYLLMPIMTTAMGAAMEMRGK"
     gene            5187..5261
                     /locus_tag="VKO45_09365"
     tRNA            5187..5261
                     /locus_tag="VKO45_09365"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5223..5225,aa:Glu,seq:ttc)
     gene            complement(5282..>6028)
                     /locus_tag="VKO45_09370"
     CDS             complement(5282..>6028)
                     /locus_tag="VKO45_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06118.1"
                     /translation="NRVSVVVFGRAPFGRDELGILKADLRSALGGAEDLAGFYRFAEK
                     DPILSTVVKHLKGMRTGGPNDLFGDVILAILLQMAPMARSSQMMDALLEQYGRRISFD
                     GREVTLWPTAAEMAKIDPKELWDKTKIGYRAERLVKAARFLSAHPISLRELSSLPEEE
                     AVKKLMEIPGVGPYSAGIILGTFPLDVWSVVIFSELFLGKTPENSRAEIADVGARLTS
                     RFGKWRWLVFVYVLQDLPYLKETYHLSRVT"
BASE COUNT         1029 a         1986 c         1969 g         1044 t
ORIGIN      
        1 ccagagaggc ccctacctcc ctggacagcc acggttcctt tggacccggg gctccctgcg
       61 ggacccggga tcctgtccag gggggcccca acccccccgt acgctcccgg ctccttcgga
      121 cccgggatcc tccacacctc ccggggcacc ttccggtacc ccggacaaac cctcctctgg
      181 cgaaggtcca tcacccccgc ctcggtggtt cccgccgcga aggaggcggt gcgtgaaggc
      241 gggtggcttg tcgcggagga gtccctcggc gtctcgggag ccggcgatgc ggcggtgctc
      301 acctcgggag atgattaccg gattgccgcg ggaaaacgct cggcccttgc cgcaaagcgg
      361 gagagcatgc gggaagttcc cctcgtggtg gtgccgccgg gggcccggtt cgatcacccg
      421 aacctggtcg attgcgggga atgcgtgagc tgggacgggg acctctgttc ctatcatcac
      481 aacggtatcc ggggtgagtt ccggaatccc ctcggggatc tgccgcccta ccgggtggcc
      541 atctccctcg cggcggccac cctctgcgcc ctcgggcggg atccgtcggc cctcgcctcc
      601 ttcgggggcg tggagggcag gctctcggtg gagaggaagg acggggtcat cgtcgtggac
      661 aacgcgaaca gcggggcaac ggccggaacg acggaggcgg ccgcctccta cgcaagggcc
      721 gtctcgggcg gcggctggat caccctcgtg attggtgagg aagaccgggc ggtctgcgag
      781 gggttcccgc cccgggacat cgactcctcg atccagaaga tccacccgga ccgggtgatc
      841 ctggtagggg aggcggcccg ggccgtacgg cccgggggat tcgggaggag gatcgcgtac
      901 gcggacaccc tcgacgcggc gagagccctt gccctccggg agacacccgc cggaggggcg
      961 gtggtcctct cggtaaagac ctggaggtga ccatggacta catccagccc cgcccctcat
     1021 ccatcgttgc ggccctctac accgcgaggg atctcgacgt agacgtggcc atcctccacg
     1081 gcccctcggg ctgctccttc aagcacgccc ggctcctcga ggaggacggg atgcgggtcc
     1141 tcaccacctc cctcgccgac cacgagttca tcttcggcgg gcaggggcct ctcgagcggg
     1201 tgctccggta cgccgaacag cggttctccc ccgcccggat ggcggtcatc gggacctgcg
     1261 tctcgatgat catcggggag gacctcccct ctgccctcgc cgggagcggg gtctccaccc
     1321 ccaccatcgt ggtgaacatc catgccggtt tccgggagaa catcgagggg gtcatcgcga
     1381 ccctggaggc cgcggcggag gcgggatgga tcggtaccgc cgagctcgag cggcagcggt
     1441 acctcctcgc ccgggcgaac gaggtggagc acctccgggg tgcggccatc caggagtaca
     1501 tcgagccctc ccggggggac ctgaagcacg tggcggcggc gaggctcctt gagatggccc
     1561 gctccggcga gcacgggatg gcggtcctga acgcgaagaa ggagaccgcc tacatgtttg
     1621 ccgacgagct cctggccctc cacgaggtct gcccggacgc cccgattacg tacctcgcga
     1681 acctcgagga aaggggcctc ccgaaggtgc gggaggacgc ccggatcatc cgggcgggca
     1741 tggtagagag gggactcacc cccggtcttc tcggggccct ggacgagtac ggggcgaacg
     1801 gagatgccat cggggaacgg atcgccgcgg aggcccctgc cttcctcctc ctggccgggg
     1861 tgcctcacgc ggtgcccccc gagtacacga aggggatcga ggtcttctcg gtgacgaatg
     1921 gcccgaggca ggtggagccc ctccggcttc tcggccacca gcacgtcgtc gtcgaggtgg
     1981 acctgcaccc gaagaccctc ggggtcaaac atatcgtcga gagcgagttc ggggcggtgc
     2041 tgaggtccct tcgatgaagg ccctcctcat ctccggcgat cgttccggga cggggaagac
     2101 ctccatcacc ctcgccctcg cctccctctt ctcggagagg accccggtcc agaccttcaa
     2161 ggcagggatg gactacatcg acccgtcgta cctctcggcg gtgaccgggc gcccgtgccg
     2221 gaacctcgac gggttcgtca tgggggaccg ggagattcag gcctccttct cccacgggtg
     2281 cctcggggcc gggctcgccc tcatcgaggg ggtccggggc ctctacgagg gggccgatgc
     2341 cctcaccgat cgggggtcca cggccgggat cgcgaaggat ctcggggtcc cggtcatcct
     2401 cgtcgtgaac gcccggtcca tcacccggtc cgcggccgcc ctcgtgaagg gcttccaggc
     2461 cttcgacccg gccatctcca tccggggggt gatcttaaac agcgtcggga gcgagtccca
     2521 cacgaggaag gcggtgaccg ccatcgagca tgcctgcggg atcccggtcc tcggtgccat
     2581 cccccaggat tcccgggtcg agctcaccat gcgccatctg ggcctcgtcc cctaccggga
     2641 gggaagcggg gacggggagt tcacgaacag gcttgccgca gttcgggaac tcgtttccgc
     2701 ccacgttgac ctggaccggg ttcttgcctg cgccgccgac ctcgatccgg tctcgtccga
     2761 cccttccccc ttcacggcgg agaaaaagcc agatgtgcgg atcggggtgg cgtacgacga
     2821 ggctttcaac ttctattacg ccgacctctt cgacctcctc cccgccctcg gggcagaagt
     2881 agtgaccttc agtccggtcc aggggagact gccggaggcc gacgggtata tcctcggcgg
     2941 gggctacccg gagctcttcg ggcccgggct cgaggcgaac gaggggatga aagagggact
     3001 gcggcaggca tctctcgatg ggaagcccat ctacggcgag tgcggcgggc tcatgtacct
     3061 gacggaccgg atcgtgctcg cgaagggctg gcgcggggga cccggggcat cctatgccat
     3121 gtgcggggtc ttccccggcg agacccggat gcccgccagc cgggtcgtct cctacgtcga
     3181 aggggttgcc tccggggaga gccccctcgg ccccggcccc ttccgggggc acgagttcca
     3241 ttacagcgac gtggtcctgg acagcggcac ccggtacgcg taccggatca cccggggact
     3301 gggcatccgg gacggccttg atggtgccct ggtccgccgc acccttggat cctacaccca
     3361 cctccacccc ctcgcgagcc ttgacatgtt cagggcgttt gtcgaggggt gccggggaaa
     3421 gaggtgaata ggtaacatcc ggacgctcca gggggactcg cttcgctcgc ccccgccgct
     3481 gcggcgtccc ccgcgtgggg ataggcctga acggaaatac ctgaaaccat atcacatatc
     3541 cccggacact gggggggtgg ccgcgccggg ggtggcgaag cccccccgag agcctgacga
     3601 agaaaatgac tgatttacca gacaggcaga ttggagcacg aattcctctt catccgtacg
     3661 atgatccaga tctcctgtcg taatgttgat cgatttcgat gcggagctac cctgatcctg
     3721 gtaagcaccc gaatcctaat tgttccgcaa gctgtaaaag cggcacgctc ctaactctct
     3781 agtcatgtgg atctacctgg atgggaggtt cgtccagaag gaggaggcga aggtctcggt
     3841 ctttgaccac gggctcctct acggcgacgg ggttttcgag ggcatccgcg cctacaacgg
     3901 gaaggtcttc cggctcgacg gccacgtcga ccggctctat gactcggcaa agaccatcaa
     3961 tctcattccc cccctctcga aggatgagat gaaggccctc atcctggaga cgatgaggag
     4021 gaacaagctc agggacggct acatccggcc ggtcatcacc cggggcgtgg gggacctcgg
     4081 cctcgacccc cggaagtgcc cgaggcctac ggtcattatc atcgccgtgg aatggggtgc
     4141 gatgtacggg gatctctatg aaaagggctt gaaggcggtc tcggtctcgg tgaggaggaa
     4201 cgccgccgaa tccctcccgc ccaatgcgaa gaccctcaac tacctgaata acatcctcgc
     4261 gaagatggag tcctatctga agggagggga cgaggccatc atcttcgaca cgaacggcta
     4321 cctatcagag ggatccgggg acaacatctt tgtcgtgaag aacggggtcc tcgccacccc
     4381 gccgtgcctg aacaacctcc gggggatcac ccagcaggtc gtcgtcgagt gcgcgaagga
     4441 gctcgggatc gaggtctggt ggcagaacat gggcttcttc gacctgtaca cggccgacga
     4501 ggttttcgtc accgggacgg ccgccgaggt cgcgcccatc gtcgagatag acggccgttc
     4561 catcggcgag gggaggccag gccccgtgac aaagaagctc atgaaggcct tccgcgaggt
     4621 cacgaagcgc gaggggacgc cgatctgaac tgtccggggg aatttccggc catcaagcct
     4681 cgtcgtctac ccctctcccg gggtgaaatc cgatggagaa gaacctcaaa ataattcttt
     4741 ccggtgtcat cgcctgggta atccccttca tcgtctcggt gcccctgtat ccgcatggac
     4801 agccgatctc cgatattcag gtcaccaagt ccatcctcat cgtcgtcggg gcactggtcg
     4861 gggcggtcct cgccctctgg tatctccggg acgtgaggca ggattttacc cgggagggcg
     4921 cgatcctggg catcgcctgg ttcgtgatca actccgctct cgacatcctg gttctcgtgt
     4981 acctcctcca cggaatggat ctcgcttcct ggacggggca gatcgggatc cgctacctcc
     5041 tcatgcccat catgacgacg gcgatggggg cggcgatgga gatgagaggg aaatagtcgc
     5101 ccccgagggc cccgctcccc aggtcccctg gagcgttcgg gaaccccgga acccttatgt
     5161 gaattcaggg aaaacggtat cccggcgctg ctatagtgta gaccggccaa tcatctcggc
     5221 ctttcacgcc gaggactggg gttcaaatcc ccatagcagc atatcagttt tttccgtctt
     5281 ctcacgtcac cctcgacagg tgataggtct ccttcaggta cgggaggtcc tggaggacgt
     5341 acacgaaaac gagccagcgc catttcccga accggctggt gagcctggcc cccacgtcgg
     5401 cgatctcggc ccgcgagttt tccggtgttt tccccaggaa gagctcggag aagatgacca
     5461 cgctccacac gtcgaggggg aatgtcccga ggatgatccc ggcggagtac ggccccacgc
     5521 cgggaatctc catgagcttc ttcaccgcct cctcctcggg aagtgacgag agctccctga
     5581 gggagatcgg gtgcgccgag aggaaccggg cggccttcac caggcgttcc gcccggtacc
     5641 cgatcttcgt cttatcccaa agttccttcg gatcgatctt cgccatctcc gccgctgtcg
     5701 gccagagggt cacctctcgg ccgtcgaagg agattcgcct cccgtattgc tccaggagcg
     5761 catccatcat ctggctgctt cttgccatcg gcgccatctg gagcaggatg gcgagaatga
     5821 cgtccccgaa gaggtcattc gggcccccgg tccgcatgcc tttcaggtgt ttcacgacgg
     5881 tagagaggat gggatccttt tcggcgaacc ggtaaaagcc ggcgagatcc tcagctcccc
     5941 cgagggctga ccggagatcg gccttcagga tcccgagctc gtcacggccg aagggggccc
     6001 ggccgaacac gacgacggat accctgtt
//
LOCUS       DATGDI010000249         4800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_231260, whole genome shotgun sequence.
ACCESSION   DATGDI010000249 DATGDI010000000
VERSION     DATGDI010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231260"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VKO45_09375"
     CDS             complement(<1..361)
                     /locus_tag="VKO45_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HMA06119.1"
                     /translation="MEAERVPTGIPALDSLLGGGLERRAITQIYGEPGCGKSTLALIA
                     AAERLRAGESVIYLDTEGFSVERFRQVAGERAEELARSLFLFEPLDFEQQGLMIAESE
                     RLLQKGGVGLLVIDSATA"
     gene            complement(351..1541)
                     /gene="larC"
                     /locus_tag="VKO45_09380"
     CDS             complement(351..1541)
                     /gene="larC"
                     /locus_tag="VKO45_09380"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HMA06120.1"
                     /translation="MRILLFDPFHGASGDMITAALLHLGADREAVRRAMASVVKEPGI
                     EEVERGGIRALRVRTGAEVHPRSWEEVRSRLSAADAPAPALALAERVMGRLHRAEGAV
                     HGGVVHFHEVGADDAIADIVGACTALHNLHAGQVAVLPVAFGRGLVRGSHGVLPVPAP
                     ATAAILSGSELEGYPGPDEGELLTPTGAALLAEFSTLPSSRIPPGKILAVGYGAGSRE
                     TGDVPNLLRAFLLETADLAADRVEILETNVDDVSPEVIAYTISRLMTAGARDASAIPS
                     VMKKGRSGHLIRVIAEPALTGPLALILAEELGTLGIRVIPTVHRLVLGRRVIRVPFSV
                     GDGTREIEVKIGSAGEKVTSVKPEFEQARNIAGELGVPVREVLRRAEEAAREIVSREG
                     GRGG"
     gene            1585..4002
                     /locus_tag="VKO45_09385"
     CDS             1585..4002
                     /locus_tag="VKO45_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HMA06121.1"
                     /translation="MPEVFLKVDSAYPEDQGAGKVRLDPETMLQLRLSPGNIVYIEGK
                     RRTVAKVWRSMVSDWHQGKVRVDQFTRANAGGLAIGDRVKVAAVEEEVEAKRVVLAPP
                     EDMPRQAPINYPGVVNRLIDYPVVKNDLIPIPAGLPFMQAQLVAFKAVVVEPENAIII
                     TKNTQVEFSDKPVAGFEGLKRVSYEDIGGLKEELQRVRETIELPMRHPELFSKLGIEP
                     PKGVLLYGPPGTGKTLIAKAVANESKAHFIPIAGPEIISKYYGESEQKLRELFEEARQ
                     NAPSIIFIDELDSIAPRREDVTGEVERRVVAQLLTMMDGLEERGQVVVIGATNRVDAI
                     DPALRRPGRFDREIEIGVPNEVDRGEILKIHTRGMPVAKDVELTKLSQQTHGFVGADL
                     AALAREGAIRALRRYLPEIDLEAEEIPAEVLNRMEVLGSDFRDALRDVGPSAMREVLL
                     EVPHTRWADVGGLDSEKEEVREAVEYPLTKRERLDRLCVEPPKGVLLYGPPGTGKTLI
                     ARAVATESGANFIPVRGPQLLSKWVGESERAVREIFKKARQVAPSIIFFDELDALAPA
                     RGASADSHVIESVLNQILTEMDGLLEMRNVVVMGATNRLDIVDPALLRTGRFDRLVYI
                     GEPAEEDRKRILSIYLGRMPIEGSAFDRLTRIVRNIPERDLEDLLEALGNDRRVTPEE
                     IAGPLADRPVPEREVANPVTCKRLSDLLANHRLILEDPVLEALVSSLAQRTAGYVGSD
                     LEGVCKEAGMLALRDGSASVTEGHFDDAVRKVRPTMNERSREYYRRMREVFKGGLPKE
                     VQPPEYQ"
     gene            4269..>4800
                     /locus_tag="VKO45_09390"
     CDS             4269..>4800
                     /locus_tag="VKO45_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06122.1"
                     /translation="MTGGTPKTGITPEAAANKRSTGIASLDHLFSGGFLKGTSIVVYG
                     TAVSGMELLGRQFWQQDTNGSYLITDAEPRQGMIDARSVSLEKYLELGRGERVVVDSL
                     STVILKYGIDTAMKFLSICVKDTEKRQSNYLFLVYKGIHTPVEEMRINRLADTVLEFR
                     EEYEGIEIQHSLIVHKI"
BASE COUNT          844 a         1610 c         1614 g          732 t
ORIGIN      
        1 gggcggtcgc cgagtcgatg acgaggagcc ccactccacc cttctggagg agccgctcgg
       61 actcggcgat catgagcccc tgctgctcga agtccagggg ctcgaagagg aagaggctcc
      121 gggcgagctc ctcggcacgc tccccggcaa cctggcggaa cctttccacc gagaatccct
      181 cggtgtcgag gtagatcacc gactcgccgg cccggaggcg ttccgccgcg gcgatgaggg
      241 caagggtgct cttcccgcac ccgggttccc cgtagatctg ggtgatggcc cggcgctcga
      301 gccctccgcc aaggagagaa tcgagggcgg gaatcccggt cgggacccgc tcagcctcca
      361 cggcccccct cccgtgagac gatctccctg gccgcctcct ccgccctgcg gagcacctcc
      421 cggaccggca ccccgagttc cccggcgata ttgcgggcct gctcgaactc cggcttcacc
      481 gacgtgactt tttccccggc tgacccgatc ttcacctcga tctcccgggt cccgtcgccg
      541 acgctgaagg gcacccggat cacccgcctc ccgaggacga ggcggtggac ggttgggatg
      601 acccggatcc cgagggtccc gagctcctct gcgaggatca gggcgagggg tccggtgagg
      661 gccggctcgg cgatgacccg gatgaggtgc ccgctcctcc ccttcttcat caccgacggg
      721 atggccgagg cgtcccgtgc gccggcggtc atgagccggg agatggtgta ggcgatgacc
      781 tccgggctca cgtcgtccac gttcgtctcc aggatctcga cccggtccgc cgcgaggtcc
      841 gctgtttcca ggaggaaggc ccggagcagg ttcggcacat cccccgtctc ccgggacccg
      901 gcgccgtagc ccaccgcgag gatcttcccg ggggggatcc gcgaagaggg gagggtggag
      961 aactcggcga ggagggcggc tcccgtcggg gtaaggagct ccccctcgtc cgggccggga
     1021 tacccctcca gctcggagcc ggaaaggatg gcggccgtcg cgggggcggg gacgggaagg
     1081 accccgtggg aaccccggac gagtcccctt ccgaacgcca cggggaggac cgcgacctgc
     1141 ccggcgtgga ggttgtggag ggcggtgcag gccccgacga tatccgcgat ggcgtcgtcg
     1201 gccccgacct cgtggaaatg gacgaccccg ccatggacgg ccccctctgc ccggtgcagc
     1261 ctccccatca cccgctctgc cagagcgagg gccggagccg gtgcgtccgc cgccgagagc
     1321 ctcgaccgga cctcctccca cgaccggggg tggacctcgg ccccggtcct cacccggagg
     1381 gcgcggatcc ctccccgctc gacctcctcg atcccgggct ccttcaccac cgaagccatg
     1441 gcccggcgca ccgcttcccg gtccgctccg aggtggagaa gggcagcggt gatcatgtcc
     1501 ccggatgccc cgtggaacgg gtcgaagagg aggatcctca tacggaagag tactttttat
     1561 ccccgcgtat atgaccttat ggtgatgcct gaagttttcc tgaaggtcga cagcgcgtac
     1621 ccggaggacc agggggccgg gaaggtgcgg ctcgatcccg agaccatgct ccagctccgc
     1681 ctctcgccgg gcaatatcgt ctatatcgag gggaagaggc ggaccgtggc gaaggtctgg
     1741 cgctccatgg tctcggactg gcaccagggg aaggtgcggg tggaccagtt cacccgggcg
     1801 aacgccggag ggctcgccat cggggaccgt gtaaaggtcg cggccgtgga ggaggaggtg
     1861 gaggcaaagc gggtggtcct cgccccgccg gaggacatgc cccgccaggc acccatcaac
     1921 tacccgggcg tcgtgaaccg gctcatcgac tacccggtgg tgaagaacga cctcattccc
     1981 ataccggccg gcctcccctt catgcaggcc cagctcgtgg ccttcaaggc ggtggtggtg
     2041 gagcccgaga acgccatcat catcacgaag aacacccagg tcgagttctc tgataagccg
     2101 gtcgccggct tcgagggctt aaagagggtg tcctacgagg acatcggcgg cctcaaggag
     2161 gaactccagc gggtgaggga gaccatcgag ctcccgatgc gccaccccga gctcttcagc
     2221 aagctcggga tcgagccgcc gaagggggtc ctcctctacg gcccgccggg gacgggaaag
     2281 accctcatcg cgaaggccgt cgcaaacgag agcaaggccc acttcatccc catcgcagga
     2341 cccgagatca tctccaagta ttacggcgag agcgagcaga agctccggga gctcttcgag
     2401 gaggcccggc agaacgcccc ctccatcatc ttcatcgacg agctggactc catcgccccc
     2461 cgccgggagg acgtgaccgg cgaggtggag cgccgggtgg tggcccagct cctcaccatg
     2521 atggacggcc tcgaggagcg gggccaggtg gtcgtcatcg gcgccacgaa ccgggtggac
     2581 gccatcgacc cggccctccg ccggccggga cggttcgacc gggagatcga gatcggggtg
     2641 ccgaacgagg tggacagggg cgaaatcctc aagatccaca cgaggggaat gcccgtcgca
     2701 aaggacgtgg agctcacgaa gctctcgcag caaacccatg gcttcgtcgg ggcagacctc
     2761 gcggccctgg cccgggaggg ggccatccgg gccctccggc ggtacctccc tgagatcgac
     2821 ctcgaggcag aggagatccc ggccgaggtc ctcaaccgga tggaggtgct cgggagcgac
     2881 ttccgggacg ccctccggga cgtcggcccg agcgccatgc gggaggtgct cctggaggta
     2941 ccccacaccc ggtgggctga cgtgggcggc ctcgattcag agaaggagga ggtgcgggag
     3001 gccgtcgagt accccctcac gaagcgggag cgcttggacc gcctctgcgt tgagcctccg
     3061 aagggggtcc tcctctacgg cccgccgggg acgggaaaga ccctcatcgc ccgggcggtc
     3121 gccaccgaga gcggggcgaa cttcatcccc gtccgtggcc cccagctcct ctccaagtgg
     3181 gtcggggaat cggagcgggc cgtccgggag atcttcaaga aggcccggca ggtcgccccc
     3241 tccatcatct tcttcgacga gctcgacgcc ctcgccccgg cccggggggc ctccgccgat
     3301 tcccatgtca tcgagagcgt cctcaaccag atccttaccg agatggacgg gctcctcgag
     3361 atgcggaacg ttgtcgtcat gggggccacg aaccggctcg atatcgtgga cccggcgctc
     3421 ctccggacgg gacggtttga ccggctggtg tatatcggtg agccggcgga ggaggacaga
     3481 aagaggatcc tctccatcta ccttgggagg atgcccatcg agggctcggc tttcgatcgg
     3541 ctcacaagga tcgtgaggaa catccctgaa cgggatcttg aggaccttct cgaggcccta
     3601 gggaacgacc gaagggtcac ccccgaggaa atcgcaggcc ccctcgcgga ccgcccggtc
     3661 ccagagagag aggttgcgaa tcccgttaca tgcaagcggc tttcagacct cctcgccaac
     3721 caccggctca tcctcgagga ccctgtccta gaggccctgg tctcctccct cgctcagcgt
     3781 acggcggggt acgtgggatc cgaccttgag ggggtctgca aggaggcggg gatgctcgcc
     3841 ctccgggacg gctcggccag cgtcaccgag ggccacttcg acgacgcggt gaggaaggtc
     3901 cggcccacga tgaacgagcg ctcccgggaa tactaccggc ggatgcggga ggtcttcaag
     3961 gggggtctcc cgaaggaggt ccagccgccg gagtaccagt aaattttttc cgaatccctt
     4021 tttctgaccc tttctgaagc cacagccgcg ggggaggcgg agcgatcccc ggaggagcat
     4081 ccgagaggag gcatcccggt tgcgaggcga cccgggatcg ggtaatcctt cccattcccg
     4141 actttttggc aaaaggagcc cgcagtaacg cccgccggcc cttcctcccc cggtccggca
     4201 aggtttataa ccgcccgggg tgacaccatc atggggagag ccgaagtcat cctcaaccgt
     4261 gatacgattt gaccgggggt acaccgaaga ccggcattac accggaggcc gccgccaata
     4321 agcgctcgac ggggatcgcg agcctcgacc atctcttctc cggagggttc ctgaagggga
     4381 cgagcatcgt ggtctacggg accgccgtct cggggatgga gctcctgggg cgtcagttct
     4441 ggcagcagga cacgaacggc tcctatctca tcaccgatgc cgagcccagg cagggaatga
     4501 tcgacgcccg cagcgtctcc ctcgagaaat acctggagct cggccgggga gagcgggtgg
     4561 tcgtggactc cctctcgacg gtgatcctga agtacgggat cgacaccgcg atgaagttcc
     4621 tttctatctg cgtgaaggac accgagaaac ggcagtccaa ctacctcttc ctcgtgtaca
     4681 aggggatcca caccccggtc gaggagatgc ggatcaaccg gctcgccgat acggtgctcg
     4741 agttcaggga ggagtacgag ggaatcgaga tccagcactc cctcatcgtc cacaagatcc
//
LOCUS       DATGDI010000250         2874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_201971, whole genome shotgun sequence.
ACCESSION   DATGDI010000250 DATGDI010000000
VERSION     DATGDI010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201971"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(629..952)
                     /locus_tag="VKO45_09395"
     CDS             complement(629..952)
                     /locus_tag="VKO45_09395"
                     /inference="COORDINATES: protein motif:HMM:NF023181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HMA06123.1"
                     /translation="MMSEDRVNHPTHYQLYYGLEVIDLTEQMNFNRGNAVKYICRAGF
                     KSTDTEIQDLQKAAFYIKREISRLEELRRLADITKFERLVDEQEECDICVQHGVKINT
                     EFLES"
     gene            complement(1573..>2874)
                     /locus_tag="VKO45_09400"
     CDS             complement(1573..>2874)
                     /locus_tag="VKO45_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06124.1"
                     /translation="DGEFAKIGVGKYCCVPGTGILTVDSWNNIGKYVGLKDTFVVFDE
                     QRLVGSGGWVKAFLKIARANHWILLSATPGDSWIDYIPVFVANGFYKNRTEFKRDHVI
                     YASWSKFPKIIGYNNERKLAFLRKKILVEMPYLRNTQRHLITVPVSHDNELYLKVVKH
                     RWDPFKNEPVTNLASLFSLSRRIVNGDLSRLSALLELMENHKKIVLFYNFDYELVILR
                     SLANLERSGSEWQTQKPADITLSSLRRSQKKSQLFVSAAKPTCPTPKNVLERTTYTTP
                     IMEPGSIFQPVERKTQTNSNQPLRTSSPQNSSSGQNDTPSLEEKFSLNGNHKSTFQIA
                     EWNGHKHEPIPKTDRWVYLVQYTAGAEGWNCTDTDTVVFYSLNYSYKLWEQCQGRTDR
                     LNTPFTDLYYYAFVSDTSIDRAIMEALESKRDFNEQVFIHH"
BASE COUNT          781 a          656 c          546 g          891 t
ORIGIN      
        1 ttataccttt cgttccaaaa tgtcgccaaa agatccactt ttgttacttt ttgtcctatt
       61 atggatgcat tttaatgtcc gttttatacc ttttattatg agttgtcagt atttgatact
      121 ttttgatatg tttcttcact aatgacgaat agcatgtgtc cattcgtctc gaaaagtata
      181 ccttcttttc tagcccactt aggaacctga acaggcgaac acaatttaat accgatccca
      241 gctgcatgtg tggatcggta aaccgtctta gttgtatcgt catatagtcg aagtgtagga
      301 ctggttcgac caatgtcctg agtgtgttga ataagacctt cgatatccac aaactccaaa
      361 ttttcaagaa aattattctt ggtattcccg tctttgtgga ttactttctt tggataactc
      421 aacgaatcgg cttcgtcaaa gaaactgcaa gcaacgatac gatgaaccaa atatgatcca
      481 taacctcgaa cgggatgctt caatgaaacc cgaaacctat tacaagattc gtgacgcttc
      541 agtagtactt tggtatcagc ctttctcaca cctccaaacg aagaaacttc atacccctca
      601 aaacctttga cgcggatcca ctcttcactc atgactcaag gaattccgta ttgattttaa
      661 caccatgttg gacgcagata tcgcattctt cttgttcatc gacaagacgt tcgaacttag
      721 taatatcagc aagcctacga agttcctcga gtctggagat ctctcgcttg atatagaatg
      781 cggctttctg caaatcttga atttcggtat cggttgactt gaatccggcc cgacaaatat
      841 acttaaccgc attaccgcgg ttgaagttca tctgttcggt caaatcaatc acttcaagac
      901 cgtagtagag ttgatagtgc gtaggatgat tcactctgtc ttcagacatc atactttcca
      961 acaatctcat aaccggttga cacttctaat gccgtgtaat acttttcgcc agtcatataa
     1021 actttctcga taagctttgt gattctaacg cacttagaaa taataccacc cattgcaaag
     1081 tacgttgtcg tctcgatcaa cccacttggg agaatcacag atagtggttc gggcaaatta
     1141 ttaggatcca atacttcagt catcgtaatg accaaatacg gtgatctttg tgctgattgt
     1201 ttcttgtccg ataagcaacg gtccgtccaa agtatcgttc atgatatacc taacgatctt
     1261 aacgaactct acgatatccc cgtcttgttt ctcataatgt actagtttaa acgtcttatt
     1321 ccccaaaaat tcactagttg gcttaggcaa attattcggg tccaacattt cgaatatacc
     1381 tttcagatat cgacaatctc atgagagata atcacacagt gagtaacttc gctcatgatc
     1441 tgtttcgcgg tcccatcttt gtcaaacacg gtgatcacca ccgtttcagg aatattacga
     1501 gtcaccgtag tggaacactt cttctttcca tcatgctcaa actcatagac cgtagtcacc
     1561 gagtcatcag gattaatggt gtatgaagac ttgctcattg aaatctcttt tcgattcaag
     1621 ggcttccata atagcccgat caatagaggt atcactcaca aacgcataat aatatagatc
     1681 tgtaaaaggt gtgtttagtc ggtcagtacg accttgacat tgctcccata gtttatagga
     1741 gtaattcaga gaatagaaaa ccaccgtatc agtatccgta cagttccatc cctcagcccc
     1801 cgccgtatac tgcacaagat aaacccagcg atcagtcttt ggaatcggtt cgtgcttgtg
     1861 accgttccac tccgctatct ggaatgttga tttgtggttt ccattcagag agaacttttc
     1921 ttctaagctc ggcgtatcgt tctgtccact cgaagaattc tggggtgaac tcgttcttag
     1981 gggctggttc gagtttgtct gtgtcttccg ctcgactggc tggaatatgc tcccaggttc
     2041 cataatgggt gttgtatatg tagttctttc caacacgttt ttcggggtcg gacacgtcgg
     2101 ttttgcagct gacacaaaca actgtgattt tttctgtgat cgacgcaatg acgacaacgt
     2161 tatgtctgca ggtttctggg tttgccattc agatccactt ctttcgagat tcgctagact
     2221 tcgaagaatc actaactcgt agtcgaagtt gtagaagagc actatcttct tgtggttctc
     2281 catcaattcc aagagggccg ataaacggga caagtcgcca ttcacaatcc tcctcgatag
     2341 gctgaatagg gatgctaggt ttgttactgg ctcgtttttg aatggatccc atctatgttt
     2401 aaccaccttt aaataaagtt cgttatcgtg gcttacaggt accgtaatga ggtgtctctg
     2461 tgtattcctg aggtatggca tctcaacgag aatcttctta cgaagaaatg ccaatttacg
     2521 ttcgttatta taacctatga tttttgggaa cttcgaccaa gatgcataaa taacatggtc
     2581 tcgtttgaat tcagtacgat ttttgtaaaa accattagcg acgaacacgg gaatataatc
     2641 aatccacgag tcgccagggg tcgcacttaa taggatccag tggttagctc gagcgatttt
     2701 caagaatgct ttgacccaac caccagaccc aacaagtctt tgttcatcga ataccacgaa
     2761 ggtatctttt agaccaacat acttaccaat gttgttccat gagtctacag ttaatatacc
     2821 tgttcctgga acacaacagt atttaccaac accaatcttg gcaaattcac catc
//
LOCUS       DATGDI010000251         1872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_260800, whole genome shotgun sequence.
ACCESSION   DATGDI010000251 DATGDI010000000
VERSION     DATGDI010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260800"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VKO45_09405"
     CDS             complement(<1..417)
                     /locus_tag="VKO45_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06125.1"
                     /translation="MSKRRPIIDSAVYPFSLFLRNNSSVSDLSEILETDTSYLYMVVK
                     GTRKYGNKIADRVNEYANEIGITIDWNLAETYDGDFFKKYNVSNSNESSNGISREEFE
                     EFKAEMNECFVGIRKELDELKSQKSKPTVFNSQPKQE"
BASE COUNT          534 a          435 c          348 g          555 t
ORIGIN      
        1 ttcttgtttg ggttgagaat taaatacagt tggtttggac ttctgagatt ttaattcatc
       61 taattctttt ctaatgccca caaaacattc attcatttca gctttaaatt cttcaaattc
      121 ttcgcgtgaa attccatttg aagattcatt agaatttgaa acattatatt ttttaaagaa
      181 atcaccatca taagtttctg ctaaattcca gtctatagtg ataccaattt cattggcata
      241 ttcgtttacc ctatctgcta ttttgttacc atatttcctg gttcccttta ccaccatata
      301 tagatatgag gtatcagttt ccagtatttc tgataaatca gacactgaag agttgttcct
      361 caagaacaga gaaaacgggt atactgcact atcaattatg ggcctacgtt tactcatact
      421 atggttcctt tcaaccaagt aaatcagcac cattgctgat agtctgtagt ataagtttat
      481 ctggaatttt agcaacacaa tatctgttaa agtgggttaa gattatctgg atttgtggta
      541 ataacatata ctgtattgtg gtagtgaata ccatgaattt tggtaacaca ttcataatac
      601 atctagacct caaaaactgg atttctggtc caaatagata ctgcacatta ggactcaaaa
      661 ctaaaatttc tgtccaaatt tatgtgggga gtttacaccc ttccactcac ccttttaggg
      721 ggtgaccctg tcaagagaat atcctataca cactgttaag aaaaaatatc cttgacagcg
      781 tgcttgccct gttgtataat gtgtgtgctg agggagacca aggcaaacca aggaggcaca
      841 catggctaac acatctggac gcttcttctt gttcatggct cttggcatcg tctctgctct
      901 gttaactgct tgcctctaat ctgccctagg gctgatccct cctagtgggc cactaggcgc
      961 tgcacacaag ctctagaccg ctaaatgggc atgccccata cgctatgacc taatagcacg
     1021 gcctagccgt ctaaagcacg ctccaagcca gtgcgtaaag ctggctcatt atcactcaat
     1081 ccagctcaat gttaagaaaa tattcccttg acagacacca ccgcagactg tatactagtg
     1141 gcatgaagga aacaaacttt caacaggagc atcacatgtc actgatcgct gaagccttcc
     1201 tcaacacaat cacactagat aacgtgttct gccacatcaa ccccgtgcgc gatgcacaag
     1261 cccttaacgc ttatttgtcc caggagaact aaatgcgaca aatcgaaatc cgcctttccc
     1321 acttcattcc tgtgcccact ccaactggca cgcagcgcaa agctcgccag ctcacacggc
     1381 ttcaaggcac ccttccagag ctgattgcta aagtccggca atattgccca caaatgccat
     1441 ctagcgaagt ttccagggcg attactgtct gcccgtggca tgactctacg ccgcttggct
     1501 cagaccactt ggttacgttc attactatcc acgagtctga aactgagccc tgtgcccaca
     1561 aagaacacgc tagaacactg gctgagcagg aagactgaaa aaccaaaaac ccgctcttag
     1621 cccggttcgt ccgggctttt gtttataaga aatttccttg acaaaaaaac caagccttgg
     1681 tggtttggat tgtcaagtat ttgttttctt tatttttaaa aagaaaaaga gaggaaggga
     1741 gtggaagggg agaggagtag tataggaact ctctaggtat ataagcgaac ttttttttta
     1801 aatattgttt tgtctccttg atttttcttg acaattaaaa ttcagttcga ccttgacaaa
     1861 ctcttctctt cc
//
LOCUS       DATGDI010000252         9615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_85954, whole genome shotgun sequence.
ACCESSION   DATGDI010000252 DATGDI010000000
VERSION     DATGDI010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9615
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85954"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..399
                     /locus_tag="VKO45_09410"
     CDS             70..399
                     /locus_tag="VKO45_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HMA06126.1"
                     /translation="MKTEVLQAIKKAEEEYQAEIAGAKAEKERRLSAATLEADTLVMK
                     AGTGSEEYKKKRLEDVRREAARKREEILREGERKAALLREQSGKNLDGAVRLLLSRFE
                     AEVYVKD"
     gene            386..2347
                     /locus_tag="VKO45_09415"
     CDS             386..2347
                     /locus_tag="VKO45_09415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HMA06127.1"
                     /translation="MLKTKSMTHLLITGLKERLEETISELYLQHVFHIREYVEGKDPE
                     YEGFVLGRPLAGAGSISEELVRLRGVISLLGIQREEVPAKERPKAGDVRGRMERDLPP
                     LEKEVEDLVSRRRDILEPRVRELEGEVASLTPFAAAPLDLDLYRGYTSLAVFAGKIGH
                     DVNVPVPHEKFFTMAVDGNFIALFAKNEDRAGVEGILQEARFQPLPIPEGSGSPGKRL
                     GACREDLARARKETGEIDGRLGELRSQHEVFLLACEELLSTQAEQFEAPLRFATTEGA
                     FVAEGWVPTERVKAIKEGVARAAGGKVYITELEMGHETREVPVEYENPPFAQPTELIT
                     DLHSRPRYSEVDPTLPVAIIFPIFFGIILGDVGYGAILLAAALALPKVLASKEAARLS
                     TVLRNCSISSIIFGLLFSEVFGFEFPWHPILFSRHLVIGGEAGEGPNIVGLLVFAVWI
                     AVAQITLGRVLNAVNQSRGHHGVKGVLGQVGWIATMFGILSLIWSLFPIPYMPNFTHL
                     PPLVMGLSLSALAGVVMIIAGIALIAMESSLELIELPTIISNSMSYARLAAVGLSSVV
                     IAIVINYIAIAKLIEPNLETLTFMGIIMILIGIAVLVVGHLLNTALGLIGGGLQSLRL
                     QYVEFFTKFYKGGGKKYRPFGLEKKFSED"
     gene            2350..2628
                     /locus_tag="VKO45_09420"
     CDS             2350..2628
                     /locus_tag="VKO45_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HMA06128.1"
                     /translation="MALPIEPSVVPTLAGLTTEMITASQVGTKALAASIAISTTAFAT
                     ALAEYAIGTAAVGATAEKPEVFGSVLILTVIPETIVIFGLVVALLLII"
     gene            2641..3219
                     /locus_tag="VKO45_09425"
     CDS             2641..3219
                     /locus_tag="VKO45_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HMA06129.1"
                     /translation="MELEAVAADIRDKARRDAEAIRDETRRETERILSSAQERAGEIR
                     LGAEEDVKRQAARMADLEISSANLAVKRLLLNTQKDLLDQVYRATLDAIGRNPESFRR
                     DTMKNLLVRARKEIPEGAVFCSGRDAQALQSILSSGTEFSGFRFGGSVDIPGGVIIES
                     KSGQLKIDYSYRMFLDRVWEAGLKDASDVLFR"
     gene            3229..4287
                     /locus_tag="VKO45_09430"
     CDS             3229..4287
                     /locus_tag="VKO45_09430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HMA06130.1"
                     /translation="MALVSAPGSYIYVCTRLRVRRSSLLPREDLLRMLHMELSEIVRF
                     IGETGYKKEIGELSTLCAGTDLDLIEMALSWNMAKEFQKILEIVPEGLRGFTSAYLRR
                     WDVQNVINILRGKSQGMKAGKIKEVLIPAGDLDREFLDRLIAEDSVEKVVAALSVVRF
                     AHIIEGVFIAGITDETLMTLENELIKNYYEDLAVVAGSGVKGGDIFLDWLRMEIDFKN
                     IQTLFRLREYREKGEVLSLMIPRGGIPVADLVRLNGIEARGEFIDALKKKAGLEPLQA
                     ALEDLRKERPIHEIEIALTRALLHQMSFMAKMHPFSIYPVLSYLEEKKYEVANLRTLA
                     RGKKVNIPTEEIKRYLVI"
     gene            4288..4590
                     /locus_tag="VKO45_09435"
     CDS             4288..4590
                     /locus_tag="VKO45_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HMA06131.1"
                     /translation="MEIAVIGSGEFVLGFRLAGIKKTYAAEGEERLKAAIQRAMDDPE
                     VGILVLSTVDFERIPVRLRTTLENSIKPTVVAIGSEEGGISLREKIKRSVGVDLWK"
     gene            4607..6343
                     /locus_tag="VKO45_09440"
     CDS             4607..6343
                     /locus_tag="VKO45_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HMA06132.1"
                     /translation="MRNQGILKRIAGPVVTAVGMDAHMYDVVKVGKEELMGEVIKIQE
                     EMTIIQVYEDTSGIRPGEPVVNTGLPLSVELGPGLLKSIYDGIQRPLEVLITKQGNFI
                     SRGVTAPGLDRAKKWEFKPVVKTGDRVGPGSILGEVQETNIVHRILVPPRAKVSTVKS
                     IRPGSFTVEEVVCVLEDGTQVTMMQKWPVRVSRPVKEKLNPDVPLLTGQRILDGLFPI
                     AKGGTAAIPGPFGSGKTVTQQQLAKWSDSEIVVYVGCGERGNEMTEVLTEFPKLVDPK
                     SGKPLMERTVLIANTSNMPVAAREASVYTAVTIAEYFRDMGYDVSLMADSTSRWAEAM
                     REISSRLEEMPGEEGYPAYLAARLSEFYERAGRVVTLNGKNGSVSVIGAVSPPGGDFS
                     EPVTQNTLRIVKVFWALDAKLSQRRHFPSINWLNSYSLYLDSLQPWYDREVSPEWNKV
                     RAWAMEILEKEAELQEIVQLVGSDALPEEQQITIEVARMVREVFLQQNAYDPVDTYCD
                     MGKQFAMMTAIRKYSEHAYAAQAAGVLLPQIIAVKSKGELPQIKFIKDYRPALEKIMS
                     AMEAEFAAMRAG"
     gene            6343..7776
                     /locus_tag="VKO45_09445"
     CDS             6343..7776
                     /locus_tag="VKO45_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HMA06133.1"
                     /translation="MKEYKTITQIAGPLVFVEKTEPVGYGELVNIILSDGTMKRGQVL
                     DTSDELVVVQVFETTAGIGRDSGIRFTGETIKMPVGKGMLGRILSGGGKPIDGGPEIV
                     PEKRLDITGAAINPYARHSPEEFIQTGISTIDGMNTLVRGQKLPIFSGAGLPHNDIAL
                     QIARQAKVLGSKEPFAVVFAAMGITKEEANHFMADFERTGALERAVVFLNLADDPAVE
                     RIITPRLALTTAEYLAFELSMHVLVILTDMTNYCEALRQIGAAREEVPGRRGYPGYMY
                     TDLASIYERAGLIHGVKGSITQFPILTMPGDDITHPIPDLSGYITEGQLVISRELHRK
                     GIYPPINVLPSLSRLMNLGIGAAHTREDHKKVSDQCYAAYAEGNDLRGLVAIVGKEAL
                     SERDRLFLEFADQFEDRFVRQGANEDRSIVQTLDLSWELLSMLPVEQLVRIDRPLIEK
                     YHPKFRGGKGAPGDEKKGAPAAAAPQG"
     gene            7783..8412
                     /locus_tag="VKO45_09450"
     CDS             7783..8412
                     /locus_tag="VKO45_09450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HMA06134.1"
                     /translation="MAIRDIKPTRSELIQLKRKIKLSERGYNILKMKRDGLILEFFNV
                     LEKAKTTREDLQEKYKKAARMSDLANTVEGSINLKAAAFSVKETPSITLKPKNIMGVV
                     VPQIEASKVRKGLFDRGYGLLATTSVMDETATAYEELVDSIVRAAEIETTIKRLLDEI
                     ESTKRRVNALEFKVIPELTEARDFIKMRLDEMEREELFRLKKIKATHKD"
     gene            8418..>9615
                     /locus_tag="VKO45_09455"
     CDS             8418..>9615
                     /locus_tag="VKO45_09455"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HMA06135.1"
                     /translation="MAGSIGTLDEVRWAGRTVLLRLDLNSPIDPFSNQILDDKRFREH
                     LPTIERLRDAKLVIITHQSRPGKKDFTTLRAHAERLSRLLRRPVRYVDDIIGARAKDA
                     VKDLSTGDILMLENVRFSAEENLKMKPEEAAKTLLVQGLAAMGDLFVNDAFGTAHRSQ
                     PTMVGLPLALRSVAGLLMEKEVSMLSRALAGTQRPVTAVMGGTKVDDSIAVMAHMLDH
                     GIADAVIVTGVVANVFLIARGYNIGEPSTQLIHKLEYLKEIELARELLERHGEKIHMP
                     AWVAVRKDGERAEFPVDAIPGDAPVLDLGVDAIEEMAKRIRSSGTVIFNGPAGVFEEE
                     AFALGTVELLNAATHVPFSVIGGGHTGAMVEKLGLEGKITHVSTGGGACIEFLTGRKL
                     PAVAALE"
BASE COUNT         1876 a         3011 c         3102 g         1626 t
ORIGIN      
        1 accaaatgac aacacatata ttgcaacttt tctccagttt ataggtacca acaccggaag
       61 gagcgtggta tgaagactga agtcttacag gcaatcaaga aggcagagga ggagtaccag
      121 gccgagatcg ccggggcgaa ggccgagaag gagcgccggc tctctgcggc aaccctcgag
      181 gctgacaccc tggtgatgaa ggcagggacc ggatccgagg agtacaaaaa gaagcgcctg
      241 gaggacgtga ggagagaggc agcccggaag cgtgaagaga tactcaggga gggtgaacgg
      301 aaggccgctc tcctgcggga gcagagcgga aagaacctgg acggggcggt gaggctcctt
      361 ctctcacggt tcgaggcgga ggtgtatgtt aaagactaag tccatgaccc accttcttat
      421 cacgggtctc aaggagaggc tggaggagac catcagcgaa ctctacctcc agcacgtctt
      481 ccatatccgg gagtacgtgg aagggaagga cccggagtac gagggtttcg tcctggggag
      541 acccctcgcg ggagccgggt ccatatccga ggaactcgtc cggctccggg gtgtgatctc
      601 cctcctcggg atccagcgtg aggaagttcc cgcaaaggag aggccaaagg ccggggatgt
      661 ccgcgggagg atggagcgcg acctcccgcc tctggagaag gaggtcgagg acctcgtctc
      721 ccggaggcgg gatatcctgg agccgagggt ccgcgagctc gaaggggagg tggcatccct
      781 cacgcccttc gcggccgcgc ccctggacct ggatctgtat cgcgggtaca cgagccttgc
      841 ggtctttgcc ggaaagatcg ggcatgacgt gaacgtaccg gtcccccatg agaagttctt
      901 caccatggcg gtcgatggta acttcatcgc cctctttgcg aaaaacgagg accgggcagg
      961 ggtggaaggg atcctccagg aggcccgttt ccagcccctc cccatccctg aagggtcggg
     1021 atccccggga aagcgcctcg gggcatgcag ggaggacctc gccagggcaa ggaaggagac
     1081 gggggagatc gacggccgcc tcggcgagct gcgctcgcag cacgaggtat tcctccttgc
     1141 ctgcgaggag ttactctcca cccaggcaga gcagttcgag gcacccctcc ggtttgccac
     1201 gaccgaaggg gccttcgtgg cagagggctg ggtccccacc gaacgtgtga aggccatcaa
     1261 ggagggggtg gcgagggcgg cagggggaaa ggtgtacatc accgagctcg agatgggcca
     1321 cgagacgagg gaggtcccgg tggaatacga gaaccccccc tttgcgcagc ccaccgagct
     1381 catcaccgac ctccactcga ggccccggta ctcggaggtc gatccgaccc tcccggtggc
     1441 catcatcttc cccatcttct tcggcatcat cctcggggat gtggggtacg gtgccatctt
     1501 gcttgccgcc gccctcgccc tcccgaaggt ccttgcatcg aaggaggctg cacggttatc
     1561 caccgtgctg cggaactgca gcatctcctc catcatcttc ggacttctct tttccgaggt
     1621 cttcggattc gagttcccct ggcaccccat cctcttctcc cggcaccttg tcatcggcgg
     1681 agaggccggg gaggggccga acatcgtcgg gctcctcgtc tttgcggtct ggatagccgt
     1741 cgcccagatc accctgggga gggtgctcaa tgccgtgaac cagtcccgcg gccatcacgg
     1801 cgtgaagggg gtcctgggac aggtggggtg gatcgccacg atgttcggga tcctctccct
     1861 gatctggtcc ctcttcccca tcccctacat gccgaacttc acccatcttc ccccgctcgt
     1921 catggggctc tccctgtccg ctctcgcggg ggtggtgatg atcatcgccg ggatcgccct
     1981 catcgccatg gagtcttctc tcgagctcat cgagctcccc accatcatct ccaactccat
     2041 gtcctacgca cggctggcgg cggtgggcct ctcgtcggtg gtcatcgcca tcgtcatcaa
     2101 ttacatcgcc attgcaaagc tcatcgaacc taatctggaa actcttacct tcatggggat
     2161 catcatgatc cttatcggga ttgcagttct tgtcgtcggg cacctgctga acacggcact
     2221 ggggctcatc ggtgggggac tgcagtcgct gagattgcag tatgtcgagt tcttcaccaa
     2281 gttctacaag ggtggaggaa agaagtaccg tccatttggt ttggaaaaga aattttcgga
     2341 ggattgacta tggcacttcc cattgaacca tccgttgtcc cgactcttgc gggactgacc
     2401 acagagatga tcaccgcatc gcaggtagga acaaaggctc tcgcggcctc catcgccatc
     2461 agcacaaccg cctttgccac ggcgctcgca gagtacgcca tcgggaccgc agccgtcggg
     2521 gccaccgcgg agaaaccgga ggtcttcggt tcggtcctca tcctcacggt catcccggag
     2581 accatcgtca tcttcggcct cgtcgtggcg ctcctcctca tcatctgaag ggagcgggag
     2641 atggagctcg aagcggtcgc cgctgacatc cgtgacaagg cgaggcgtga cgccgaagcc
     2701 atccgggacg agacccggag ggagaccgag aggatcctct cctccgcgca ggagcgtgca
     2761 ggggagatca ggctcggggc agaggaggat gtgaagcggc aggcagcccg catggcggat
     2821 ctggagatat cgtccgcgaa ccttgccgta aaaaggttgc tcctcaatac ccagaaggat
     2881 ctcctggacc aggtgtaccg ggccaccctt gacgccatcg gccggaaccc cgagagcttc
     2941 cggagggata ccatgaaaaa cctcctcgtg agggcaagaa aggagatccc ggagggggcg
     3001 gttttctgta gcgggcggga tgcccaggcg ctgcagtcga tcctctcctc ggggaccgag
     3061 ttctcgggat tccggttcgg aggaagcgtc gatatcccgg gcggggtcat catcgagagc
     3121 aagagcggcc agttgaagat cgactacagc taccgcatgt tcctcgaccg ggtctgggag
     3181 gcagggctca aggatgcatc cgacgtcctg ttccggtgag aggtgggcat ggctctggta
     3241 agcgccccgg gttcgtacat ctacgtatgt acccggctgc gggtccgacg gtcctcgctc
     3301 ctgccccggg aggatctcct ccggatgctt cacatggagc tctcggagat cgtgaggttc
     3361 atcggggaga ccggctacaa gaaggagatc ggggagctct cgacgctctg cgcgggaacg
     3421 gacctcgacc ttatcgagat ggccctctcc tggaacatgg ccaaggagtt ccagaagatc
     3481 ctggagatcg tcccggaggg gcttcgcggg ttcacctccg cctatctgag gcgctgggat
     3541 gtccagaacg tcattaacat cctccggggc aagtcccagg ggatgaaggc gggcaagatc
     3601 aaggaggtcc tcatcccggc cggggatctc gaccgggagt tcctggaccg tctcattgcc
     3661 gaggattcgg tggaaaaggt cgtcgcggca ctctcggttg tgaggttcgc ccatatcatc
     3721 gaaggggtct ttatcgcggg gatcacggat gagaccctca tgaccctgga gaacgagctg
     3781 atcaagaact attacgagga tctcgccgtc gttgcaggga gcggggtgaa ggggggggat
     3841 atcttcctcg actggctcag gatggagata gacttcaaga atatccagac cctcttcagg
     3901 ctccgggagt acagggagaa aggggaggtc ctctccctca tgatcccccg gggcgggata
     3961 ccggtggcag atctcgtcag gttaaacggc attgaggccc ggggggagtt catcgacgcc
     4021 ctgaagaaga aggccggcct cgaacccctc caggcggccc ttgaggatct caggaaggag
     4081 cggccgatcc atgagatcga gatcgccctc acccgggccc tcctccacca gatgagcttc
     4141 atggccaaga tgcatccctt ctccatctac cccgtcctca gctacctgga ggagaagaag
     4201 tacgaggtcg ccaacctgcg gacccttgcc cggggcaaga aggtgaacat ccccaccgag
     4261 gagatcaagc ggtacctggt gatctgagtg gagatagctg ttattgggag cggggagttc
     4321 gtcctgggat tccggctcgc ggggatcaag aagacgtacg ctgccgaggg tgaggagagg
     4381 ctgaaggctg ccatccagcg ggccatggac gacccggagg tcggtatcct ggtcctctcc
     4441 accgtcgact tcgagaggat accggtgcgc ctccggacca ccctggagaa ctccatcaag
     4501 ccgacggtgg tggccatcgg gagcgaggag ggggggatct ccctccgaga gaagatcaag
     4561 agatcggtgg gtgttgacct gtggaagtga caatcgaggg atatctatga ggaatcaggg
     4621 aattctcaag cggatcgcag gccctgtcgt caccgcggtg gggatggatg cccacatgta
     4681 tgacgtggtg aaggtgggga aggaggagct catgggagag gtcatcaaga tccaggagga
     4741 gatgaccatc atccaggtgt acgaggacac ctcgggcatc cggcccgggg agcccgtggt
     4801 gaacaccggc ctccccctgt cggtggagct cgggccgggc ctcctcaaga gcatctacga
     4861 cggcatccag aggcccctgg aggtgctcat caccaagcag gggaatttca tctcccgggg
     4921 cgtcacggcg cccggccttg accgggcgaa gaaatgggag tttaagcccg tcgtcaagac
     4981 aggggacagg gtaggaccgg gttcgatcct cggcgaggtc caggagacga acatcgtcca
     5041 ccggatcctc gtaccgccca gggcaaaggt cagcacggtg aagtccatca ggccggggtc
     5101 atttaccgtg gaggaggtcg tctgcgtcct cgaggacggg acccaggtca ccatgatgca
     5161 gaagtggccc gtccgggtct cccggccggt gaaggagaag ctcaacccgg atgtcccgct
     5221 cctcaccggg cagcggatcc tggacggcct cttccccatc gcgaagggcg gcacggcggc
     5281 catccccggt cccttcggga gcgggaagac cgtcacccag cagcagctcg ccaagtggtc
     5341 ggactcggag atcgtggtct atgtcggctg cggcgagcgg gggaacgaga tgaccgaggt
     5401 gctcaccgag ttcccgaagc tcgtggaccc gaagagcggg aagcccctca tggagcggac
     5461 ggtgctcatc gcgaacacga gcaacatgcc cgtcgcggcc cgggaggcct cggtgtacac
     5521 ggcggtcacc atcgccgagt acttccggga catgggatac gacgtctccc tcatggccga
     5581 ctccacctcc cggtgggccg aggcgatgcg ggagatctcc tcccggctcg aggagatgcc
     5641 gggcgaggag ggctatcccg cgtacctcgc ggcacggctc tcggagttct acgagcgtgc
     5701 gggccgggtc gtcaccctga acgggaaaaa cggttcggtc tcggtcatcg gagccgtctc
     5761 accgcccggc ggggacttct cggagccggt cacccagaac acgctgcgca tcgtgaaggt
     5821 cttctgggcc ctcgatgcga agctctccca gcgccggcac ttcccgtcaa tcaactggct
     5881 caactcctac tccctgtacc tggactccct ccagccctgg tacgaccggg aagtctcccc
     5941 cgagtggaac aaggtccggg cctgggccat ggagatcctc gagaaggagg cggagctcca
     6001 ggagatcgtc cagctcgtgg gatcggacgc cctccccgag gagcagcaga tcaccatcga
     6061 ggtcgcacgg atggtgcggg aggtcttcct ccagcagaac gcctacgatc cggtggacac
     6121 ctactgcgac atggggaagc agttcgccat gatgaccgcg atccggaagt actccgagca
     6181 tgcctacgcg gcccaggcgg ccggtgtcct cctcccccag atcatcgcgg tgaagtccaa
     6241 gggcgagctc ccccagatca agttcatcaa ggactaccgg ccggcgctcg agaagatcat
     6301 gtcggccatg gaggccgagt ttgcggcgat gagggcggga taatgaagga atacaagaca
     6361 atcacccaga tcgccgggcc cctcgtcttc gtggagaaga cggagcccgt cgggtacggc
     6421 gagctcgtga acatcatcct ctccgacggc accatgaagc gagggcaggt acttgacacg
     6481 agcgacgagc tcgtcgtcgt ccaggtcttc gagacgaccg ccgggatcgg cagggattcc
     6541 ggcatccgct tcaccggtga gaccatcaag atgcccgtgg gcaaggggat gctcggccgg
     6601 atcctttcgg gaggcgggaa gcccatcgac gggggccccg agatcgtccc ggagaagcgc
     6661 ctggacatca cgggtgcggc catcaacccc tatgcccgcc actcccctga ggagttcatc
     6721 cagacgggca tctcgaccat cgacgggatg aacaccctcg tccgggggca gaagctcccc
     6781 atcttctcgg gcgcgggcct cccccacaat gacatcgcgc tccagatcgc ccggcaggcc
     6841 aaggtgctcg gttccaagga gcccttcgcc gtggtctttg ccgcgatggg gatcacgaag
     6901 gaggaggcga accacttcat ggccgacttc gagcggacgg gggccctcga gcgtgccgtg
     6961 gtgttcctga acctcgccga cgatcccgcc gtggagcgga tcatcacccc ccggctcgcc
     7021 ctcaccaccg ccgagtacct ggcctttgag ctctccatgc acgtgctcgt catcctcacg
     7081 gacatgacga actactgcga ggccctccgg cagatcgggg cggcccggga ggaggttccc
     7141 ggccggcgcg ggtaccccgg ctacatgtac actgacctcg cctccatcta cgagcgggca
     7201 gggctcatcc acggcgtgaa ggggtccatc acccagttcc ccatcctcac catgcccggg
     7261 gacgacatca cccaccccat ccccgacctc tcgggctaca tcaccgaggg ccagctcgtc
     7321 atctcccggg agctccaccg gaaggggatc tacccgccca tcaacgtgct cccctccctc
     7381 tcccggctca tgaacctggg gatcggggcc gcccacacga gggaggacca caagaaggtt
     7441 tcggaccagt gctacgcggc ctatgccgag gggaacgacc tgcggggcct cgtggccatc
     7501 gtcgggaagg aggccctctc ggagcgggac cggctcttcc tcgagttcgc cgaccagttc
     7561 gaggaccggt tcgtccggca gggtgcgaac gaggaccggt ccatcgtcca gaccctggac
     7621 ctttcctggg agctcctctc gatgctgccc gtcgagcagc tggtccgtat cgaccggccc
     7681 ctcatcgaga agtaccaccc gaagttccgg ggcgggaaag gggcgccggg ggacgagaag
     7741 aagggggcgc cggcagccgc tgcgccccag gggtaacccc ccatggccat ccgggacatc
     7801 aagcccaccc gctcggagct catccagctg aagcggaaga tcaagctctc cgagcggggc
     7861 tacaatatcc tgaagatgaa gcgggacggg ctgatcctcg agttcttcaa cgtcctcgag
     7921 aaggcaaaga ccacccgcga ggatctccag gagaagtaca aaaaggcggc gaggatgagc
     7981 gacctcgcga acaccgtcga ggggtccatc aacctgaaag cggccgcgtt ctcggtgaag
     8041 gagaccccct ccattaccct gaaaccgaag aatattatgg gggtcgtggt tccccagatt
     8101 gaggcatcga aggtgagaaa gggcctcttc gaccggggtt acggcctcct cgctaccacc
     8161 tcggtcatgg acgagacggc caccgcctac gaggagctcg tggactccat cgtccgggcg
     8221 gcggagatcg agaccaccat aaagcggctc ctcgacgaga tcgagtccac gaagcgccgg
     8281 gtgaacgccc tcgagttcaa ggtcatcccc gagctcaccg aggcccggga cttcatcaag
     8341 atgcgcctcg acgagatgga gcgcgaggag ctcttccggc tgaagaagat caaggcgacc
     8401 cacaaggact gacggcgatg gcgggatcca tcgggaccct ggacgaggtg cggtgggccg
     8461 gtcgtacggt cctcctccgc ctcgacttaa actcccccat cgatcccttc tcgaaccaga
     8521 tcctcgacga caagcgcttc cgggaacacc tccccaccat cgagcgtctc cgggatgcga
     8581 agctcgtcat catcacccac cagtcacggc cggggaagaa ggacttcacc accctccggg
     8641 cccatgccga gcggctgtcc cggctcctcc gccggccggt ccggtacgtg gacgacatca
     8701 tcggggcccg ggcaaaggac gccgtgaagg atctctccac cggcgatatc ctgatgctgg
     8761 agaacgtccg gttctcggcc gaggagaacc tgaagatgaa gcccgaggag gcggcaaaga
     8821 ccctcctcgt ccaggggctc gccgccatgg gggacctctt cgtgaacgat gctttcggca
     8881 cggcccaccg ttcccagccc accatggtcg gcctccccct cgccctcagg tcggtggccg
     8941 gtctcctcat ggagaaggag gtctccatgc tctcccgggc cctcgccggc acccagaggc
     9001 cggtgaccgc ggtcatgggc gggaccaagg tggacgactc catcgccgtg atggcccaca
     9061 tgctcgacca tggcatcgcc gacgcggtca tcgtgaccgg cgtggtcgcc aatgtcttcc
     9121 tcatcgcccg ggggtacaac atcggcgagc cctccaccca gctcatccac aagctcgagt
     9181 acctgaagga gatcgagctc gcccgggagc tcctggaacg ccacggggaa aagatccaca
     9241 tgcctgcctg ggttgcggta cggaaggatg gagagcgggc ggagttcccc gtggatgcca
     9301 tcccggggga tgccccggtc ctcgacctcg gcgtggatgc catcgaggag atggcgaaaa
     9361 ggatccggtc gtcgggaacg gtcatcttca acggcccggc cggggtcttc gaggaggagg
     9421 ccttcgccct cgggaccgtc gagctcctga acgccgcgac ccatgtcccc ttctcggtca
     9481 tcggcggtgg ccacaccggc gccatggtgg agaagctcgg cctcgagggg aagatcaccc
     9541 acgtctctac cggtggcggg gcctgcatcg agttcctcac gggaaggaag ctcccggcgg
     9601 ttgcggccct cgaga
//
LOCUS       DATGDI010000253         9906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_434994, whole genome shotgun sequence.
ACCESSION   DATGDI010000253 DATGDI010000000
VERSION     DATGDI010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434994"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VKO45_09460"
     CDS             complement(<1..376)
                     /locus_tag="VKO45_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06136.1"
                     /translation="MPSSGDVRLRVALVPVGEVDPVVISWVKEDLPGIFDAEVEVSPG
                     IPLAGRHLVKGRNQYLADGILGDFSSMVRAEGTALLGITEADLFSPGLNFVFGIANRG
                     RALISTFRLREEFYGPARKPDGF"
     gene            complement(360..1319)
                     /locus_tag="VKO45_09465"
     CDS             complement(360..1319)
                     /locus_tag="VKO45_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HMA06137.1"
                     /translation="MPRIAVAEGDGIGKEVIPVARKVLETVRPDIEFFPVEVGYGRWE
                     RTGEAITPAEIQAMKKADAVLFGAVTTPPDPNYRSVVVRMRKELDLYANIRPVKGGNA
                     DLVIVRENTEGLYSGIEEIGPDRATSLRLITRKGSERIARFACNLAEGRRHLTIGHKA
                     NVLKSDTLFREVCIRIAEETGVAYREKFIDALCLDILQHPRNYDVIVTENMFGDILSD
                     VAAYLVGGLGMLPSANIGDRWALFEPVHGSAPKHAGRNTANPVAAIRSAAMLLEHLGD
                     RKNALKIEQAVDQALTSGVKTRDLGGTVGTREMGDAVIDALKR"
     gene            complement(1297..2118)
                     /locus_tag="VKO45_09470"
     CDS             complement(1297..2118)
                     /locus_tag="VKO45_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06138.1"
                     /translation="MIFPEHCKEVGYITGKPCGDRVYFLSRYMIRERNGEYEILEVEQ
                     SPGERGMMRGVASLRTIAGGKDVVMYPSPVNLHDRTNLLNLALESGHRCTIFRGLDEH
                     TTFVCDPDLSIVLTLHVYDLVPPPRPHLSATIRELERDGLFGEISVGFAHHVRDIRKT
                     DADVYPCRAAGFQHTLDSDRVLKGERVAGCMTGAQILEECGGPEVRLEEICPLESVRE
                     EPFIARCCRSEREGVRAFKGRFGGVVHWGATPWTIAETVRKVAEGWRTHAENRGR"
     gene            complement(2115..2585)
                     /locus_tag="VKO45_09475"
     CDS             complement(2115..2585)
                     /locus_tag="VKO45_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HMA06139.1"
                     /translation="MYGEGRAVCVGRDMDTDRIIAGRYLRTKDRSLWAAHVFEDLDPG
                     LAHRLRGTVVMAGSNMGCGSSREQAAIALKEAGVVGVVAPSFARIFFRNAINIGLPLV
                     ECPLTCREGARVRFNLEEGWAELDGERCTARPLSPRMLEILRAGGLVPYWRSRR"
     gene            complement(2575..3795)
                     /locus_tag="VKO45_09480"
     CDS             complement(2575..3795)
                     /locus_tag="VKO45_09480"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HMA06140.1"
                     /translation="MATLSERILGAPAGTYVDVRVDRAFAHDGTGVLTREAFCQMGCE
                     GVHDPERTYLFFDHIAPANNTTTATLQAELRSFARAKGLSFSDIGRGICHQLLAEGIV
                     LPGEVVVGADSHTCTLGAFGAFATGVGATDMAAIWASGGTWFRVPETIAIRISGGFTG
                     AAEPKDLALAYVRDLGMDGANYRALEFIGDGVMGIPMHGRLTCSNMAVEAGAKAGLFY
                     ADGETVRYLAGFGIEANPQVHEECTYVRQLEIDITDLPPLIASHHRVDRVQEVAAMAG
                     THIDEVFVGTCTNGRLEDLERLARILKGKEVRVRTIVVPASQRVLREATRKGIVEILL
                     SAGCVVGIPGCGPCLGAHMGVLGEGEVCLSTANRNFTNRMGVGGEILLSSVATAGVSA
                     LRGEVAMPEAADVR"
     gene            complement(3795..4922)
                     /locus_tag="VKO45_09485"
     CDS             complement(3795..4922)
                     /locus_tag="VKO45_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase family protein"
                     /protein_id="HMA06141.1"
                     /translation="MKPWHVEICDVTLRDGEQTPGVSFTREEKMNIAQILDQVGVEVI
                     EAGFPITSPYEKQCVTAIAGQGLNARICCLARARKEDIDAALDCDVDMVSVFIATSDL
                     HIRTKYRKPREQVLAGALTMVEYAHDHGLQVRFAAEDASRTDPAFLKEVFTGGAEHGA
                     DLLSFADTVGFLTPLQVRAIVTEMLSWTDKPLCIHCHNDLGCASANTITAAETGAFQL
                     HTTVNGIGERAGNAALEEVLVACRLKGGVERYNLTPVNRLSQVVEAYSRIPVGKTKAV
                     VGENAFAHESGIHIAAILEDPLTYEFVPPELVGRERRFILGKHSGRHALEHEVARIGY
                     HLSDTQLCSVLDKVKVYSEHKCTISDEILLELVKNVQKGSP"
     gene            complement(4894..6180)
                     /locus_tag="VKO45_09490"
     CDS             complement(4894..6180)
                     /locus_tag="VKO45_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMA06142.1"
                     /translation="MKGEEVLAKAIRRSADRFYTVPGHPVTGLARLLSAELVTNEKVG
                     LEYALGDSLQDRRAAVVVKNVGLNACADPLVNATTQGLRAGVVVVAGDDLEAKGSQNV
                     QDSRYYGELAQVPVLEPDGATCAAAVEEAFRASERFSRVAILRVTTPLLEGDCDPGLV
                     DRENRTGSLADPELTMRGRTMAADMRTRELFRWSTESPLNRLGSRKGGSVGAAAGKGE
                     GRIVTVYPPPAGADRLSGLTELGRPFLREHRFLEPPPDPGQPERFSTRGHFRTFCPSC
                     PFKGVMATLKEKGLRAICDMGCSVLAMNPPYQVGVAAYGLGSSVAVAARSTGIALCGD
                     FALLHSGVNALVDTYEKHLPLLCIVLANNRLGMTGGQPGYDPVPYIGWANPVIIPADH
                     PDLSRHIRKGKGPRTVIVEGNCPEGETYETVACGNL"
     gene            complement(6177..7145)
                     /locus_tag="VKO45_09495"
     CDS             complement(6177..7145)
                     /locus_tag="VKO45_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA06143.1"
                     /translation="MEWKRVKAELLAANTVRLEGEPCTGFISRSTAGPTAGGEGSVFF
                     SQGGRRVRLSVSRNGRVRLHHLGKGRVTLNLGDIEIEGTLEPAGLHCPRQAYVNVSER
                     CIFRCRYCTVPLQAGGIKDPEAIVARVREVLDRVDAIAITSGVSDSLETEEQRVLNLV
                     KRLVPLGKPVGVSIYPLPGTPGRLHDLGVSEVKWNLEAATPELFARMCPGLDYEAIWK
                     ALRDSVKLFGRGRVYSNVIFGLGETDEELERCIEDLTSSGIIPVLRPLNPAAGLASVP
                     RPTAERILKVCGILEKALRRHGLSTKMARTMCTACTGCDLVPGRDT"
     gene            complement(7127..8035)
                     /gene="mmp11"
                     /locus_tag="VKO45_09500"
     CDS             complement(7127..8035)
                     /gene="mmp11"
                     /locus_tag="VKO45_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker protein 11"
                     /protein_id="HMA06144.1"
                     /translation="MEKEHPGRITEPYIIHYPQIVGVADPTGARVEVVEFFDCVGGAM
                     WAKHHYRKSPLVESARTVGATTRYVLRAGSARLQLQGSTFAAGISEARVEGNEIAVTY
                     VGLGGGGVGASICRSTAKGVNRCISDPSGGGKVAGSTVWLPRRERVLIGIDDTDTPEE
                     GATWTLAHNIAKAVEDDRSRYLSHTLVQLFPVPYRTKNCVATVCEFATSDPESLVGKF
                     HRLLRKYTLSEKTGMGVFRGFDPSSLLDFARRVKRGEVTDADLGLAVRDSVDIVMDGR
                     GKIGAVAAIPFYTDYDEALVAWNGNG"
     gene            complement(8560..9450)
                     /gene="fhcD"
                     /locus_tag="VKO45_09505"
     CDS             complement(8560..9450)
                     /gene="fhcD"
                     /locus_tag="VKO45_09505"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HMA06145.1"
                     /translation="MEINGVKIDDTYAEAFPIFVSRVIVTAATRNWALKAATEATGFA
                     TSCIGCAAEAGIDHYMAPGETPDGRPGYAILICNPKKDKLKEQLIERIGECILTAPTT
                     AVFNGITDAPEKLPVKLHFFGDGYEHQVKVGDRSCWAIPIMEGEFITEEEFGVVKGVA
                     GGNFFIMGENQMAALTGAEVAAEAIETVRGAILSFPGGIVASGSKVGSKKYAKFMKAS
                     TNEAYCPTLREKVPDTKVPVGIGAIYELVIDGVDAKAIEAAMRAGIKAACMVPGVKVI
                     SAGNYGGKLGPHQFKLHSIL"
     gene            9718..>9906
                     /locus_tag="VKO45_09510"
     CDS             9718..>9906
                     /locus_tag="VKO45_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA06146.1"
                     /translation="MAFAVHVNLDRCTGCNNCVVACPVNALELHTVDPVTKEKIYCVR
                     DGKAITLDVKSELCAGCGV"
BASE COUNT         1782 a         3250 c         3024 g         1850 t
ORIGIN      
        1 gaaaaccatc aggttttcga gccggcccgt agaactcttc gcgaagccgg aaggtcgata
       61 tcagggccct cccccggttc gcgataccga acacgaagtt cagtccaggt gagaagaggt
      121 ccgcctcggt aatcccgagg agggcggtcc cctcggcccg gaccatcgag gagaaatccc
      181 cgagaatccc atcggcgagg tactggtttc gtcccttcac gagatgcctg ccggcgagag
      241 ggatgccggg ggagacctcg acctctgcat cgaagatgcc ggggaggtcc tcctttaccc
      301 aggagatcac gacgggatcc acttcgccga caggaaccag ggcaactctc agccgcacgt
      361 caccgcttga gggcatcgat gacggcatcc cccatctccc ttgtccccac cgttcccccg
      421 agatcgcggg tcttcacgcc cgatgtaagg gcctgatcta ccgcctgctc aattttcagg
      481 gcgttcttcc ggtccccgag atgttcgagg agcatggcgg cgctccggat ggctgcgacc
      541 gggttcgccg tgttcctccc cgcgtgtttc ggggcgctcc cgtggaccgg ttcgaagagg
      601 gcccaccggt ccccgatgtt tgccgagggg agcatgccga gtcctccgac gaggtacgcg
      661 gcgacgtctg agaggatgtc cccgaacatg ttctcggtca cgatgacgtc gtagttgcgg
      721 ggatgctgga ggatgtcaag gcagagggcg tcgataaact tctcacggta tgcgaccccg
      781 gtctcctccg cgatcctgat acagacctcc cggaagaggg tatccgattt caggacattg
      841 gccttgtggc cgatggtcag gtgcctccgg ccctcggcga ggttgcaggc aaagcgggcg
      901 atccgttcgc tcccctttct cgtgatgaga cggagcgacg tggcacggtc cgggccgatc
      961 tcctcgatac cggagtagag cccctccgtg ttctcccgga cgatcacaag atccgcatta
     1021 ccccccttta ccggcctgat attggcatag agatcgagct ccttcctcat ccggactaca
     1081 acgctccggt agttcggatc gggcggtgtg gtcaccgccc cgaagaggac ggcatcggcc
     1141 ttcttcatgg cctgaatctc cgcaggtgtg atggcctccc ccgtcctctc ccatcgcccg
     1201 taccctacct ccacggggaa gaattcgata tccggacgga ccgtctcgag aaccttccgg
     1261 gctaccggga tgacctcctt ccctatcccg tcaccttcag cgaccgcgat tctcggcatg
     1321 ggtcctccat ccctccgcca ccttccttac cgtttccgcg atggtccagg gggtcgcccc
     1381 ccagtggacg acgccgccga accggccttt aaaggccctt acgccctccc gctcgctccg
     1441 gcagcaccgg gcgataaacg gctcctcccg aaccgattcg agggggcaga tctcctcgag
     1501 gcgcacctcc ggtccgccgc actcctcgag aatctgggct ccggtcatgc accctgccac
     1561 cctttcaccc ttgaggaccc ggtcggaatc cagggtgtgc tggaaccctg cggcccggca
     1621 gggatatacg tcggcatcag tcttccggat atcccttaca tggtgtgcga atcctacaga
     1681 gatttctcca aagagaccgt ccctctcgag ctcccggatc gtggccgaga ggtggggcct
     1741 gggaggcggc accaagtcat agacgtggag agtcaggacg atcgaaagat ccgggtcgca
     1801 gacgaaggtg gtgtgctcat cgaggccgcg gaagatggtg caccggtgcc cagactcaag
     1861 ggcgagattc agcaggttcg tacgatcgtg gagattcacc ggcgagggat acatcaccac
     1921 gtccttccct ccggcaatgg tcctcaggga agcgactcca cgcatcatcc cccgctcccc
     1981 tggggactgc tcaacctcga ggatctcgta ttccccgttt cgctccctga tcatgtaccg
     2041 ggagaggaaa tacaccctgt ccccgcaggg tttaccggtg atatatccca cttccttgca
     2101 atgctcgggg aagatcaacg gcggctcctc cagtagggca cgagcccccc tgctctgaga
     2161 atctcgagca tcctcggtga gagtggccgg gcagtgcacc gctccccgtc cagctccgcc
     2221 cacccctcct ccaggttgaa ccggacccgg gcgccctcgc ggcaggtgag ggggcattcc
     2281 accaggggga gcccgatgtt gatggcgttc ctgaaaaaga tccgggcaaa gctcggggcg
     2341 acgaccccga ccaccccggc ctccttcagg gcgatggctg cctgctcccg cgatgacccg
     2401 cagcccatgt tcgatccggc catcacgacg gtgcccctga gccggtgggc gagaccggga
     2461 tccagatcct cgaagacatg ggccgcccag agtgacctgt ccttggtccg gagatagcgc
     2521 cctgcgatga tccggtccgt gtccatgtcc cggccgacgc agacggccct gccctcaccg
     2581 tacatcagcc gcctccggca tcgcgacctc cccgcggagg gccgagaccc ccgcggtggc
     2641 caccgaggaa aggaggatct ctcccccgac ccccatccgg ttcgtgaagt tgcggttcgc
     2701 cgtggacagg cagacctccc cctccccgag gacccccatg tgggccccga ggcacggccc
     2761 gcaccccgga attccgacga cgcagccggc cgacagcagg atctccacga tccccttccg
     2821 ggtcgcctcc ctgaggaccc gctgcgatgc cgggaccacg atggtcctga ccctcacctc
     2881 ctttcccttg aggatccggg cgaggcgctc caggtcctcg agccggccgt tcgtgcaggt
     2941 gccgacgaat acctcgtcga tgtgcgtccc tgccatggcg gccacctcct gcaccctgtc
     3001 cacccggtga tggctcgcga tcaggggtgg taaatcggtg atatcgatct ccagctgacg
     3061 gacataggta cactcctcat gaacctgtgg attcgcctcg atcccgaatc ctgccaggta
     3121 ccggacggtt tcaccatcgg cgtagaagag gcccgccttt gccccggcct ccaccgccat
     3181 gttggagcag gtgagtctcc cgtgcatggg gattcccatc actccgtcgc cgatgaactc
     3241 gagggcccgg tagttcgccc cgtccatccc gagatcccgg acatacgcga gggcgaggtc
     3301 tttcggctcg gcggctccgg tgaaccctcc cgagatccgg atggcgatgg tctccgggac
     3361 ccggaaccag gttccgcccg aggcccagat cgccgccatg tccgttgccc cgacgcccgt
     3421 ggcaaaggcg ccgaaggccc ccagggtgca ggtgtgggag tcggccccca ccaccacctc
     3481 cccgggaagc acgatcccct ctgccaggag ctggtggcag atcccccggc cgatatccga
     3541 gaaggagagc cccttcgccc gggcaaagga cctgagctcg gcctggaggg tggcggtggt
     3601 cgtgttgttc gccggcgcga tgtggtcgaa gaagagatag gtcctctccg gatcgtggac
     3661 cccctcgcac cccatctggc agaaggcctc ccgggtgagc actcccgtgc cgtcgtgggc
     3721 gaaggcccgg tccacccgga catcgacgta ggtcccggcc ggggcaccca ggatgcgctc
     3781 ggagagggtt gccatcaggg ggaccccttc tgcacgttct tcacgagctc caggaggatc
     3841 tcgtcggaga tggtgcactt gtgctcgctg tacaccttca ccttgtcgag gacgctgcag
     3901 agctgggtgt cggagaggtg gtagccgatc ctcgcgacct cgtgctccag ggcgtggcgt
     3961 cccgagtgct tcccgaggat gaacctccgt tccctcccca cgagctcggg ggggacgaac
     4021 tcgtaggtga gggggtcctc gaggatggcc gcgatgtgga tgccgctctc gtgggcaaag
     4081 gcattctccc cgaccacggc cttggtcttc ccgacgggga tccgggaata ggcctccacg
     4141 acctgggaga ggcggttcac cggggtgagg ttgtaccgct ccacgccccc cttcagccgg
     4201 caggcgacga ggacctcctc cagggcggcg ttccccgccc gctccccgat gccgttcacc
     4261 gtggtatgga gctggaaggc acccgtctcg gccgcggtga tggtgtttgc cgaggcgcac
     4321 ccgaggtcgt tgtggcagtg gatgcagagg ggcttgtccg tccaggagag catctcggtg
     4381 acgatggccc ggacctggag aggggtgagg aacccgacgg tgtcggcgaa ggagaggagg
     4441 tctgccccgt gctccgcccc gccggtgaag acctccttca ggaaggccgg atcggttctc
     4501 gacgcgtcct ctgcggcaaa cctcacctgg agaccatggt catgggcgta ctcgaccatg
     4561 gtcagggcgc cggcgaggac ctgctcccgg ggtttccggt acttggtcct gatgtgcagg
     4621 tcggaggtcg cgatgaaaac cgagaccatg tccacgtcgc agtcgagggc tgcatcgatg
     4681 tcctccttcc tcgcccgggc aaggcagcag atgcgtgcat tgagcccctg ccccgcgatg
     4741 gcggtcacgc actgcttctc gtaaggcgag gtgatgggga atcccgcctc gatgacctcc
     4801 accccgacct ggtcgaggat ctgggcgatg ttcatctttt cctcgcgggt gaaggagacc
     4861 cccggggtct gctcgccgtc cctgagcgtt acgtcacaga tttccacatg ccacggtttc
     4921 atacgtttcc ccctcggggc agtttccctc gacaatcacg gttctcggcc ccttcccctt
     4981 ccggatgtgg cgggaaaggt ccggatgatc ggcagggatg atcaccgggt tcgcccaccc
     5041 gatgtagggg accggatcgt atcccggctg tcccccggtc atcccgagcc gattgttcgc
     5101 aagaacgatg cagaggagcg ggagatgctt ctcgtaggtg tccacgagcg cattcacccc
     5161 cgagtggagg agggcgaagt ccccgcagag ggcaattccc gtcgacctcg cggccaccgc
     5221 cacggatgac ccgagaccat aggccgccac cccgacctga tagggcgggt tcatggcaag
     5281 aacggaacag cccatgtcgc agatggcccg gagccccttt tctttgaggg tggccatcac
     5341 ccccttgaac gggcaggagg ggcagaaggt ccggaaatgc ccccgggtgg agaatctctc
     5401 gggttgtcct ggatcgggag gcggttcgag gaagcggtgc tcccggagga agggccggcc
     5461 cagctcggta aggccggaga gtctgtccgc acctgcagga ggcgggtaca ccgtgacaat
     5521 ccggccttca cccttcccgg ccgccgcacc gacggagccc cccttccggg atcccaggcg
     5581 gttcagaggg ctctccgtgg accagcggaa gagttcacgg gtcctcatgt cggcggccat
     5641 cgtccggccc cgcatggtca gctcgggatc tgcgagggat ccggtcctgt tctcgcggtc
     5701 caccagcccc gggtcgcaat ccccttcgag gagaggggtg gtgacccgga ggatggcgac
     5761 acgggagaac ctctccgatg cccggaatgc ctcctccacc gccgcggcgc aggtcgcccc
     5821 gtcgggctcc aggacgggga cctgggcgag ctccccgtaa taccgtgaat cctggacatt
     5881 ctgggagccc ttggcctcca ggtcgtcacc cgccaccacc accactcccg cccggagccc
     5941 ctgggtggtg gcattcacga gcgggtcggc gcaggcattc agccccacgt tcttcaccac
     6001 cacggcggct ctccggtcct gcagcgaatc gccgagagca tattcaagcc ctaccttctc
     6061 gtttgtgacg agctctgcag atagaagcct cgcaaggccg gtgaccgggt ggcccgggac
     6121 cgtgtagaac cggtcggcgg agcgccggat cgccttcgca agaacctcct cgcccttcat
     6181 gtatccctcc cggggacgag atcgcatccc gtgcatgcgg tgcacatggt ccgtgccatc
     6241 ttcgtggaaa gcccgtgccg gcgaagcgcc ttttcaagga tcccgcagac cttgaggatc
     6301 cgttctgccg tcggccgggg gaccgaggcg agcccggcgg cagggttcag gggcctgaga
     6361 accgggatga tccccgagga ggtgaggtcc tcgatgcacc tctccagttc ttcgtcggtc
     6421 tctccgaggc cgaagatgac gttggaatac accctccccc tcccgaagag cttcaccgaa
     6481 tccctgaggg ccttccagat ggcctcgtaa tccaggccgg ggcacatgcg ggcgaagagt
     6541 tccggtgtcg cagcctccag gttccatttc acctcggata cacccaggtc atggaggcgc
     6601 ccgggtgtcc cgggcaacgg gtagatagac accccgaccg gtttcccgag aggtacgagg
     6661 cgctttacca ggttgaggac ccgttgttcc tcggtctcca gcgagtcaga gaccccggag
     6721 gtgatggcga tggcatccac ccggtcaagc acctcccgga cccgggctac gatggcctca
     6781 gggtccttga tcccgccggc ctgcaggggc acggtgcagt atcggcagcg gaagatacac
     6841 cgttcgctca cgttcacata ggcctggcgg gggcaatgga gtccggcggg ctcgagggtc
     6901 ccctcgatct cgatatcccc gaggttgagc gtcaccctgc ccttcccaag gtgatgcagc
     6961 cttacccggc cattcctgga gacggagagc cgcacccgcc ggccaccctg cgaaaagaag
     7021 accgaaccct caccccctgc cgtcggcccg gcagtcgagc gggagatgaa accggtgcag
     7081 ggttcaccct ccagcctcac cgtgttcgcc gcgaggagct ccgcctttac ccgtttccat
     7141 tccatgcaac cagcgcctcg tcgtaatccg tgtagaaggg gatagctgcc acggccccga
     7201 ttttccccct cccgtccatc acgatatcca ccgaatcccg gacggcgagg ccgagatcag
     7261 catccgtcac ctcgccccgc ttcaccctcc gggcgaagtc aaggaggctc gaggggtcaa
     7321 acccccggaa gacccccatt ccggtcttct ccgagagggt gtacttcctg agcaggcggt
     7381 ggaatttccc gaccagggat tccgggtctg aggtggcgaa ctcgcagacc gtggcgacgc
     7441 agttcttggt ccggtaaggg accgggaaga gctggacgag ggtgtgggag aggtaccggg
     7501 accggtcgtc ctcgacggcc ttcgcgatgt tatgggcgag ggtccaggtc gccccctcct
     7561 ccggtgtatc ggtgtcgtcg atcccgatga ggaccctttc ccgccgggga agccagacgg
     7621 tggatcctgc gacctttccc ccgcccgaag ggtcggagat gcagcggttc acgccttttg
     7681 ccgtcgagcg gcagatggat gcccccacgc ctcccccgcc cagaccgaca taggtgacgg
     7741 cgatctcgtt cccctccacc cgggcctccg agatgcccgc ggcgaaggtc gagccctgga
     7801 gttggaggcg ggccgaaccg gcccggagga cgtagcgggt ggtggcaccg acggtccggg
     7861 ccgattcgac gagagggctc ttccggtagt ggtgcttcgc ccacatcgcc cctccaacgc
     7921 agtcgaagaa ctccacgacc tccacccggg ccccggtggg atccgccact ccgacgatct
     7981 gggggtagtg gatgatgtag ggttccgtga tgcgaccggg atgctctttc tccatcggaa
     8041 tctccggaga ggatgggatt tgtaaacgaa attatcgtta acaaaataat cgttcacaaa
     8101 gataaaatca tcgcttcgat ttcggggaga atcatgcgga gccgatatag caaacgcccg
     8161 aaacaatgag gaggcccgtt gcaacgggtg tgacaaaaac ccccgaaagc attgaacaat
     8221 aaaatcaaat ctgacccaat ctgaataaaa ttaccccatc caactaacac ccgaatcgaa
     8281 ccataagatc aatccccgga cgctcctggg ggactcgcct tcggctcgcc ccccgccgct
     8341 tcggcgtccc ccgcatttac gataggcctc tctataccgt ggattgtagt atcggaattc
     8401 ccagacactg gggggttgcc cgtgcggtgg gggtggcgta gcccccccga gagcacccca
     8461 agaagaaaaa tcgaattatg cctggaattt ccataccagg aatcccatgt caaaatcgag
     8521 gatctcttcg ggaaatcgct attgaggtaa aaaagtggat cagaggatgg aatggagttt
     8581 gaactggtgg ggaccgagct tcccgccata gttcccggcg ctgatgacct tcacgcccgg
     8641 gaccatgcag gcggctttta tcccggcacg catcgccgcc tcgatggcct ttgcgtccac
     8701 gccatcgata acgagctcgt agatggcccc gatgcctacc gggactttcg tgtccgggac
     8761 cttctcgcgg agggtgggac agtaggcctc gttcgtggaa gccttcataa attttgcgta
     8821 tttcttggag ccgaccttgg agccgctcgc cacgatcccg ccggggaagg agaggatcgc
     8881 acccctgacc gtttcgatgg cctcggcagc gacctctgcc cccgtgaggg cggccatctg
     8941 gttctcgccc atgatgaaga agttcccgcc ggcgacccct tttacgaccc cgaattcctc
     9001 ctcggtgatg aactcgccct ccatgatggg gatggcccag caggaccggt cgccgacctt
     9061 cacctggtgc tcgtacccat cgccgaagaa gtggagtttt acggggagct tctcgggtgc
     9121 atccgtgatc ccgttgaaga cggcggtggt cggggcggtg aggatgcact ccccgatccg
     9181 ttcgatgagc tgctccttca gcttgtcctt cttggggttg cagatgagga tcgcgtagcc
     9241 cggccttcca tcgggggtct caccgggcgc catatagtga tcgatgcccg cttcagccgc
     9301 gcaaccgatg cacgaggtcg caaaacccgt ggcttcagtc gcggccttta acgcccagtt
     9361 ccgggtcgct gcggtcacga ttacgcggga aacgaatatc gggaacgctt ctgcgtaggt
     9421 atcatcgatt ttcacgccat ttatctccaa aatcattcac cccttttcac cacacattca
     9481 ccgggtccat taaagaatgt tatcatttga tctcttttcc ggaaaattcg actggaagcg
     9541 acaccattac atatatttaa taaggtccaa aaatcaaaat atatttataa atcttctgag
     9601 gcttttgcgg gagtccgggc gaaatctggc ctctgcgaat tttcagaaga tttaattacc
     9661 ggcttcgacc acctgatggt gatcgatttt aaatcgatag agctggtgcg tgcaaacatg
     9721 gcattcgctg tacacgtgaa tctggatcgt tgtaccggct gtaacaactg tgtggttgca
     9781 tgccccgtga acgcattgga gctgcacacc gtggatcccg taacaaagga aaagatctac
     9841 tgtgtgaggg atggcaaggc catcaccctg gacgtgaagt ccgagctctg cgccggatgc
     9901 ggcgtt
//
LOCUS       DATGDI010000254        12657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_57314, whole genome shotgun sequence.
ACCESSION   DATGDI010000254 DATGDI010000000
VERSION     DATGDI010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 12657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12657
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57314"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VKO45_09515"
     CDS             complement(<1..202)
                     /locus_tag="VKO45_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06147.1"
                     /translation="MKIGILYAGPFGEQMINHISLSGFGDRIVAVYELTPGKIPGGAR
                     PEDIWENPEKFIPGDLPDATCDL"
     gene            complement(199..1041)
                     /locus_tag="VKO45_09520"
     CDS             complement(199..1041)
                     /locus_tag="VKO45_09520"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HMA06148.1"
                     /translation="MSRPSGTGRITPQGGQPGDEGGKATPVPDLKEILSILLDFKGEK
                     VRSFCEGRIAEGGDPHGIFSELSAGLDEIGKGFEGTGRTRYFTSDLIVSGRNMKRALG
                     ILRPHFSPCGEKGFPPCGEKGFPPCGEKGFPPCGEKGFPPCGEKGFPRTGRGRGLVLL
                     GTVKGDVHDIGKMIFAVTLESNGFSCIDLGVDVDKEIFAERVTQDRPDILSLSALLSS
                     TLPAMREVLDELGRRGLRQQVKVIVGGHAVTGEFAREIGADAYGEDAIDGLKKCLSFV
                     GAGE"
     gene            complement(986..2410)
                     /gene="acsC"
                     /locus_tag="VKO45_09525"
     CDS             complement(986..2410)
                     /gene="acsC"
                     /locus_tag="VKO45_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HMA06149.1"
                     /translation="MKQIGPMKVYPLLPKTNCGVCGPKTCMGFAVQLCERSAALEECP
                     PLLEEKYRESLVRLREIFAPAVKEVVIGSGDHRITIGGALVLMRHELRYTNPPAIAVV
                     VDDSMTDETVMKRVQETNYFHYKYIGKILTLDAIAIRSADDSPQKYGKLVEMVAKSTQ
                     LPLILWTHSPAVMEEGVKAVHARRPLLYPATRDNWKQMGELALRYRCPLGVYDHEIRM
                     LRSIVKTLRVMGVDDLVLDPGCGFRGDLRETVSALTQIRIAAIRDEDELLGYPLAGAP
                     ATLWEKLYAEGKDVIRWKEACLAAMMVVRYVDLLMVSSASKWSTLPLVVLRNNIYNDP
                     RKPVSVEPGLRTFGEPDPKEAPVFLTSNFALTYYTVLSDIEKLDCHLLVVDTEGMSVD
                     SAVAGRKFTGAKVAEVVASSGIAEKVSHRTLIIPGLAARLKGDIEDATGWDVIVGPRD
                     SSAIPGYVKAKWYGQDHTPGWSAR"
     gene            complement(2415..3590)
                     /gene="cdhD"
                     /locus_tag="VKO45_09530"
     CDS             complement(2415..3590)
                     /gene="cdhD"
                     /locus_tag="VKO45_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/acetyl-CoA synthase subunit
                     delta"
                     /protein_id="HMA06150.1"
                     /translation="MGEDDGDLVRILLELLQASGEVELQDIAIAAEELTISFEGRAAL
                     SRLLAEELALAAPEKLCEVEYHPPVHTYPGSIVEVTLGAGRAEGGTRARPVTIGGERA
                     PAFHTFQEIPLHPPVIAGSIFDTAPPLPAPVRAFFGDALQDPCEWARLWVKKYGADLV
                     DLDLTSTDPYIKDTPVQDAARLVEDLLQAVDVPIIIGGSGNPQKDARLLPKVAEVCEG
                     ERVLLSSATLDMWKPLAEAAKRHGQLVLAWSPIDINQAKELNRNLTGYITQSQIIMDP
                     TSAALGYGLEYAYSVIERIRLAGLMGDREVQSPQAAGTANSWGAREAWKRDPTLGPRE
                     LRGPMWELTGSLAYLLAGVDLFIMLHPGAARTVKDIVGWLSGGSAPPTFRELIGVGK"
     gene            complement(3595..4989)
                     /gene="cdhC"
                     /locus_tag="VKO45_09535"
     CDS             complement(3595..4989)
                     /gene="cdhC"
                     /locus_tag="VKO45_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_function: GO:0043884 - CO-methylating acetyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HMA06151.1"
                     /translation="MSAREGMFSDIPVEVGVVYEGERVRKQQMHAEFGGPGVKEKYEI
                     LEVKGPGEVKDGKITLIGPDFAEIPEASSLPFGTEIAVSGKGVEPDMAGVMERRIHDF
                     SNWIEGYMHLNQRDAIWIRVDKRSVKKGLSLRHIGLVISRLLKSAIPLIEAVEVTFIT
                     DPAEVTRRIGRAQAIFEERDARTRGMTDEDVGVFFGCTLCQSFAPPHVCVITPQRYAN
                     CGAISWMDGRAASRVDPKGPIFEIAKGECVDPVTGEYTGVNTVVKEKSLGNVERVQLY
                     SAFGYPHTSCGCFECTTFFVPECDGLGIVHRDFRGTTPTGLSFGAIADSTAGGRQVDG
                     FHGLSFEYMRSPRFLAASGGWRRIVWLPSELKERMKGIIPADLFDAIATEKDVTTVGE
                     LVDFLRAHHHPAMEGMKAPEPVTATATNPGKEAPRLQTAPQNTVTIPGGSVSLTLKNV
                     KIHAERMIIRKQQE"
     gene            complement(4986..5522)
                     /locus_tag="VKO45_09540"
     CDS             complement(4986..5522)
                     /locus_tag="VKO45_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HMA06152.1"
                     /translation="MKGEAWLRAETPAIIRATLIEKPGVAAKLLRRAKNPVIVVGHEA
                     AGEKGSTLLDSLVRIAKAIDAPVIATGGVSAELGRRGYSPVSVMSAMEAGDRLLDPSW
                     EAAPGRSPPDYAFLLGIPFPIAAVVDAGLASFARDHLKIVSLDRRYHPHCDLSLPNQT
                     REEWEKTLEAIAREVEKG"
     gene            complement(5519..7861)
                     /gene="cdhA"
                     /locus_tag="VKO45_09545"
     CDS             complement(5519..7861)
                     /gene="cdhA"
                     /locus_tag="VKO45_09545"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/acetyl-CoA synthase complex
                     subunit alpha"
                     /protein_id="HMA06153.1"
                     /translation="MKLKVTIDELITDRLRAKGVEFNVGRVIEAWEEEPGPTPMPGIA
                     TLRSWDRTLLSKYHPFYLPFCDLCCLCTMGKCDLSGGKRGACGIDMAAQQSRIVLLAC
                     AMGAATHAGHMRHLLSRLIGSFGRDHPIDVGSSVLLEAPHARLVTGIKPKTLGDLEDI
                     AAYCEGEITQLLSCCHTGQEGDPLDLESKVLHAGMIDHVALEGSDLAQISAYSFPKGE
                     AETPLVECGPGCLDPGKPVILVIGHNVPPAIAISDAMREMGVENQIEVCGICCTALDL
                     TRYNPGARIVGALSQQLRFIRSGMADVIVVDEQCIRTDVLREAMKTGAPLIVTNAKNL
                     QGLADRTKDDPEEIVRSLVDGKEPGAAILDPDKVGEVAVRVAMEIAPSRKSSMKLPDR
                     ASLIEVAKTCTGCFSCMRACPNDREITTAVTAAASGDLAPLAALYDACIGCGRCGGAC
                     PAGLDPHDLIAGAYRLLFPAERYHIRAGRGPIQDTEIRAVGGPIVMGEIPGVVATVGC
                     SNYPGSWNDVGSMAEEFLKRRYIVVTSGCTAISIGMYRNENGEGLYDIYPGRFDAGGL
                     VNVGSCVSNAHISGAAVKIANIFAKRKLNSNYEEIADYVHHRVGAVGIAWGAMSQKAA
                     AIAAGFWRLGIPVIAGPHGAKYRRMLLGRRDREEDWFVLDARSGERVYAGPVPEHLFT
                     VAETREEAMVLVPKLCMRPNDTVKGRSIKLNHYIDLHRRFFGRMPDDLPLLVRTRADI
                     PITMKDEVVRVLDEQGWRESDRPIPDPTLLPRLVKGGKTA"
     gene            complement(7966..8733)
                     /locus_tag="VKO45_09550"
     CDS             complement(7966..8733)
                     /locus_tag="VKO45_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HMA06154.1"
                     /translation="MDDGLPRTVSIRGIVRETPKIRTFHLSESFRFTPGQFVMVWVPG
                     VDEIPMALSGKNSISVQEVGDATSAMFALNEGDLLGIRGPYGQGFSIQGMVLAIAGGI
                     GSAPLLPLAMEGHVGVFLLGAGTEKDLPFRKALAGKTALRIATDDGSEGRRGPVTVLL
                     DRVDLQAFDHICVCGPEPMMRAVLKSLHDEGIADRGQFSLHRYMKCGVGLCGSCAIDP
                     TGLRVCREGPVIRGDRLIGSEFGMYSRDGSGRRHPVP"
     gene            complement(8726..9613)
                     /locus_tag="VKO45_09555"
     CDS             complement(8726..9613)
                     /locus_tag="VKO45_09555"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HMA06155.1"
                     /translation="MIRSPSGPVTVGGVGLRNHLILAAGILGTTGASLGRILREGAGG
                     VVTKSIGPVPLEGHPGPCLVVQEGGLLNAMGLPNPSRDFAGELPALRGEPVVVSIFGG
                     DPGEFARVASWFADSAVGFELNLSCPHAEGFGSALGTDPGLVEECTRAVKALGRPTWV
                     KVTPNVTDITEIGAAAERGGADAIVAINTVKAMRISVPMGRPILGNRFGGLSGKAIFP
                     IAVRCVYELYDACDIPIVGCGGISTADNVVEMMMAGAAAVEIGTAVLDDIGIFRKIAR
                     DLYTPDGKKTEEIVGCAHG"
     gene            9667..10533
                     /locus_tag="VKO45_09560"
     CDS             9667..10533
                     /locus_tag="VKO45_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HMA06156.1"
                     /translation="MSTLAVIGVGKIGGEAAYVSALEGLVDELVLYDVNTPLLQAQVL
                     DLQDCGLDIGVSTDTAGIREADICVYAAGVSRNPAIKTRADLLGINLPIAETCLPLLK
                     GFSGVFVTVTNPMDILNYFFAMRAGIPRERCIGFGGQLDSARFRRILGSSGIRGPGYV
                     LGEHGEHQVPLFHPLGNPVDTETREKILMSLRGASMEIIKGKGGTIFGPGWHITRLIR
                     SILQGEEPPVPCSCVLDGEYGLSGCSLGVPTRIGKEGIRRIEEWPLDPWEEEHLREAG
                     AFLADLSRGLHG"
     gene            10526..>12657
                     /locus_tag="VKO45_09565"
     CDS             10526..>12657
                     /locus_tag="VKO45_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="HMA06157.1"
                     /translation="MGEGGSLGALPGFEKRTVARTLSDFSGIRIAINQVEYTNSPQGP
                     LIHIFGRDGNGSAVHLEVTGFRPYFYAPFEEVAGGPLPSGLEIETDREYRSIRGDTLS
                     RMYTRRPTDVRDFRERFHHFEADIPFATRFLIDTGLTGGVEAPSVQTDYRSLRPAEVN
                     APARVCIFDIECEDERGLPEPDRDKIIAVTCWDSFQDDYTSFVFPAGGGVPLAPQAGE
                     KITVEVYREEKEMLLALARYLKERDPDVLSGWNILEFDIPYIIARMEILGLSPTLLAR
                     LEERTERVALRGRALFDLLDGYKKMHGSQRESYRLDSVAEEEVGETKIRFTGTLSSLW
                     REDPGQLVDYNRKDVELCVAINRKNHIVEFYREIARYVGCPLDRTLNSSSVIDIYILR
                     KASGRFVLPSKGFAAAEEFEGATVFDPTRGVKENVVVLDLKSLYPMAMMTINASPETK
                     DPDGEYRAPNGVRFRREPDGLTRSIIAELLKEREQKKKLRDGYPFGSPEFVLYDLQQN
                     VLKVIMNTYYGVSGYARFRLYDREIGSAVTSVGRAIIEHTRTVIEGMGYHVIYGDTDS
                     CMVQIPSMETADTIAAARKIEAAVNASYREFAKKTLNADSHYFSIKFEKIYRRFFQAG
                     KKKRYAGHLVWKEGKDVDEIDIVGFELRRSDYPQITKEVQGKVIGMILKGEGFPEVKA
                     YLGEIIKNFRAGKYSLDEVGIPGGIQKAL"
BASE COUNT         2126 a         4187 c         4120 g         2224 t
ORIGIN      
        1 tcaggtcgca ggtggcatcg gggaggtcgc cggggatgaa cttctcggga ttctcccaga
       61 tgtcctccgg ccgagccccg cccgggattt tcccgggggt gagttcgtac accgccacga
      121 tccggtcgcc gaaaccggag agggagatgt ggttgatcat ctgctccccg aaggggccgg
      181 cgtagaggat cccgatcttc attcccctgc tccgacgaag gagaggcact tcttcaggcc
      241 gtcgatagca tcctcgccat aggcgtccgc cccgatctcc cgggcgaact ccccggtcac
      301 ggcgtggccc ccgacgatga ccttcacctg ctgcctgagt cccctcctcc cgagctcgtc
      361 gagcacctcc ctcatggcgg gaagggtgga ggagaggagg gcggagaggg aaaggatgtc
      421 gggccggtcc tgcgtcaccc gctcggcgaa gatttccttg tccacgtcca caccgagatc
      481 gatgcaggag aagccgttac tctcaagggt cacggcgaaa atcatcttcc cgatgtcatg
      541 gacatccccc ttcaccgtcc ccaggaggac gaggcccctg ccccgcccgg tcctcgggaa
      601 acctttctca ccgcaaggtg ggaagccttt ctcaccgcaa ggtgggaagc ctttctcacc
      661 gcaaggtggg aaacctttct caccgcaagg tgggaagcct ttctcaccgc aaggtgagaa
      721 gtggggccgg agaatgccga gggccctttt catgttccgg ccggaaacga tgaggtccga
      781 tgtgaagtag cgggtccggc ctgttccctc gaatcccttc ccgatctcgt cgagacctgc
      841 cgagagctcg gagaagatcc cgtggggatc cccgccctcc gcgatccttc cctcgcagaa
      901 ggacctcacc ttctccccct tgaagtcgag gaggatggag aggatctctt tcaggtctgg
      961 aaccggcgtc gctttccccc cttcatcacc gggctgacca ccctggggtg tgatcctgcc
     1021 cgtaccactt ggccttgaca tagcccggga tggcagacga gtccctcggc cctacgatga
     1081 cgtcccagcc ggtcgcgtcc tcgatgtccc ccttcaggcg tgccgcgagg ccggggatga
     1141 tcagggtccg gtgggagacc ttctcggcga tcccggagga tgcaaccacc tcggcgacct
     1201 tcgcccccgt gaactttctc ccggccaccg ccgagtcgac gctcatcccc tcggtgtcca
     1261 ccacgaggag gtggcagtcg agcttctcga tgtccgagag cacggtgtag taggtgaggg
     1321 cgaagttcga ggtgaggaag accggggcct ccttcgggtc gggctccccg aaggtccgga
     1381 ggccgggctc caccgagacg ggcttccggg ggtcgttgta gatattgttc cgcagcacga
     1441 cgagggggag ggtggaccac ttcgaggccg atgagaccat gaggaggtcc acataccgca
     1501 ccaccatcat cgccgcgagg caggcctcct tccaccggat gacgtccttt ccctcggcat
     1561 agagcttctc ccagagggtg gccggcgcgc cggccaaggg gtaccccagg agctcgtcct
     1621 cgtcccggat ggccgcgatc cggatctggg tgagggcgct caccgtctcc cgcagatctc
     1681 ctcggaaccc gcagcccggg tcgaggacca ggtcgtccac ccccatgacc cggagggtct
     1741 tcacgatgga acggagcatc cggatctcgt ggtcatagac cccgaggggg caccggtacc
     1801 ggagggcgag ctcccccatc tgtttccagt tgtcccgggt cgcggggtag aggaggggcc
     1861 gccgggcatg gacggccttc actccctcct ccatcaccgc ggggctgtgg gtccagagga
     1921 tgagggggag ctgggtcgac ttggccacca tctccacgag cttcccgtac ttctgcgggc
     1981 tgtcatccgc cgacctgatg gcgatcgcgt ccagggtgag gatcttcccg atgtacttgt
     2041 agtggaagta gtttgtctcc tggacccgct tcatcaccgt ctcatcggtc atgctgtcgt
     2101 cgaccacgac ggcgatagcc ggcgggttcg tgtaccggag ttcgtggcgc atgaggacga
     2161 gggcgccgcc gatggtgatc cggtggtccc cgctcccgat gaccacctcc ttcaccgccg
     2221 gggcaaagat ctcccggagc cggaccaggc tctcccggta cttctcctcg agaaggggcg
     2281 ggcattcctc gagggcggcg ctccgctcgc agagctggac cgcgaacccc atgcaggtct
     2341 tcggcccgca gaccccgcag ttggtcttcg ggagaagcgg gtagaccttc atcgggccga
     2401 tctgcttcat gccctcactt ccccaccccg atgagctccc ggaaggtcgg gggtgccgag
     2461 ccaccggaga gccatcccac gatatccttc acggtccggg ccgcgccggg gtggagcatg
     2521 atgaagaggt caacccccgc gaggaggtag gcgaggctcc cggtaagctc ccacatcggg
     2581 ccccggagtt ccctcggccc gagggtggga tccctcttcc aggcctcccg ggctccccag
     2641 gagttcgcgg tcccggccgc ctgcggggac tggacctccc ggtcgcccat gagcccggcg
     2701 agccggatac gctcgatgac gctgtaggcg tactcgaggc cgtacccgag ggcggcagag
     2761 gtcgggtcca tgatgatctg ggactgggtg atgtagccgg tgaggttccg gttcagttct
     2821 ttcgcctggt tgatgtcgat gggggaccag gcgaggacca gctggccgtg ccgcttcgcc
     2881 gcctcggcaa gaggcttcca catatcgagg gtcgccgagg agagaaggac ccgctccccc
     2941 tcgcagacct cggccacctt cgggaggagc cgggcgtcct tctgggggtt ccctgatccc
     3001 ccgatgatga tagggacgtc gaccgcctgg aggaggtcct cgacgagccg ggccgcgtcc
     3061 tgcaccgggg tgtccttgat gtagggatcg gtgctcgtga ggtccaggtc cacgaggtcg
     3121 gccccgtatt tcttcaccca gagcctcgcc cactcgcagg ggtcctggag ggcatccccg
     3181 aagaatgccc tgaccggggc ggggaggggc ggtgctgtat cgaagatgga ccccgcgatg
     3241 accgggggat ggaggggaat ctcctggaag gtgtggaagg cgggggccct ttctcccccg
     3301 atggtgaccg gccgggcccg ggtgcctccc tcggcccgcc ccgccccgag ggttacctcc
     3361 acgatggagc ccgggtaggt gtggaccggc gggtgatact ccacctcgca gagcttctcg
     3421 ggagccgcga gggcgagctc ctcggcgagg agccgggaaa gggccgcccg cccctcgaag
     3481 ctgatggtga gctcctcggc ggcgatggct atgtcctgga gctcgacctc tcccgaggcc
     3541 tggaggagct cgaggaggat tcttaccagg tccccgtcgt cctctcccat cccctcactc
     3601 ctgctgcttc cggatgatca tccgctcggc atggatcttc acgttcttca gggtgaggct
     3661 cacgcttccc ccggggatgg tcaccgtgtt ctggggggcg gtctgcaatc gcggggcctc
     3721 cttcccggga tttgtggcgg tggccgtgac cggttcgggc gccttcatcc cttccatggc
     3781 gggatggtgg tgcgccctga ggaagtcgac gagctctccc accgtggtca cgtccttctc
     3841 ggtggcgatg gcgtcgaaga gatcggccgg gatgatgccc ttcatccgct ccttcagctc
     3901 cgaggggagc cagacgatcc gccgccatcc gccgctcgcg gcgaggaacc tcggggacct
     3961 catgtattcg aaggagaggc cgtggaagcc gtccacctgc cttcccccgg cggtcgaatc
     4021 ggcgatggcg ccgaaggaga gaccggtcgg ggtcgtgccc cggaagtcgc ggtgcactat
     4081 cccgaggccg tcgcactcgg ggacgaagaa ggtggtgcac tcgaagcagc cgcaggaggt
     4141 gtgggggtac ccgaaggccg agtagagctg gacccgctcc acgttcccga gggatttttc
     4201 cttcacgact gtgttgaccc ccgtgtattc cccggttacc gggtccacgc actcgccctt
     4261 cgcgatctcg aagatgggtc ccttcgggtc gacccgggac gcggccctcc cgtccatcca
     4321 ggaaatggcc ccgcagttcg cgtaccgctg gggggtgatg acgcagacat gagggggagc
     4381 aaaggactgg cagagggtgc agccgaagaa gaccccgacg tcctcgtcgg tcattccacg
     4441 ggtgcgggca tcccgctcct cgaaaatcgc ctgcgccctc ccgatacgcc gggtcacctc
     4501 cgccggatcg gtgatgaagg tcacctcgac ggcctcgatg agggggatag cactcttcag
     4561 gagccgggaa atgacgaggc cgatgtggcg gagcgagagg ccctttttca ccgacctctt
     4621 gtccacccgg atccagatgg catcccgctg gttcaggtgc atgtagccct cgatccagtt
     4681 ggagaagtca tggatcctcc gctccatgac cccggccatg tccggctcga ccccttttcc
     4741 ggagaccgcg atttcggttc cgaaggggag gctcgaggcc tcggggatct ctgcgaagtc
     4801 gggcccgatg agggtgatct ttccgtcctt tacctctccc ggccccttca cctccaggat
     4861 ctcgtacttt tccttcaccc cggggccgcc gaattcggca tgcatctgct gcttccggac
     4921 ccgctcccct tcgtagacca ccccgacctc gacggggata tccgagaaca tcccctccct
     4981 cgcgctcatc ccttctccac ctccctcgcg atggcctcca gggtcttctc ccattcctcc
     5041 cgggtctggt tggggaggga gaggtcgcag tgggggtggt accgccggtc gagggacacg
     5101 attttcaggt ggtcccgggc aaaggacgcg aggcccgcat cgacgaccgc ggcgatgggg
     5161 aaggggatcc cgagcaggaa ggcgtagtcc ggggggctac ggccgggcgc agcctcccat
     5221 gaagggtcca ggagccggtc gcctgcctcc atggcagaca tgacggacac cgggctatat
     5281 cccctccttc cgagttcagc cgaaaccccg ccggtggcga tcacgggagc gtcgatggcc
     5341 tttgcgatcc ggacgaggga atcgaggaga gtggatccct tctcccccgc ggcctcgtgg
     5401 cccaccacga tgaccgggtt ctttgctctc ctcaggagct tcgcggccac cccgggcttc
     5461 tcgatgaggg ttgccctgat gatcgccggg gtctccgccc ggagccaggc ctcgcccttc
     5521 acgcggtttt ccctcccttc acgagcctcg ggagaagggt gggatcgggg atgggccgat
     5581 cgctctccct ccagccctgc tcgtcgagaa cacggaccac ctcgtccttc atggtgatgg
     5641 gaatgtctgc cctcgtccgg acgaggagcg ggagatcatc cggcatgcgc ccgaagaatc
     5701 ttcggtggag gtcgatgtaa tggttcagct tgatggacct ccccttcacc gtgtcgttgg
     5761 gccgcatgca gagcttgggc acgagcacca tggcctcctc cctcgtctcc gccacggtaa
     5821 agaggtgctc cgggaccggg ccggcataga cccgctcccc gctccgggca tccaggacga
     5881 accagtcctc ctcccgatcc cttctcccga ggagcatccg cctgtacttg gccccgtggg
     5941 gaccggcgat gaccgggatg ccgaggcgcc agaaccctgc ggcgatggcc gcagccttct
     6001 gggacatggc cccccaggcg atgccgaccg ccccgacccg gtggtggacg tagtcggcga
     6061 tctcctcgta gttggagttg agcttccttt tcgcaaagat gtttgcgatc ttcacggcgg
     6121 ccccggagat atgggcgttc gagacgcagg agcccacgtt cacgaggcca ccggcatcga
     6181 accgtcccgg gtagatgtcg tagagcccct cgccgttctc gttccggtac atcccgatgg
     6241 agatagccgt gcaccccgag gtgaccacga tatagcgccg cttcaggaac tcctcggcca
     6301 tggagccgac gtcgttccag ctccccgggt agttcgaaca tcctacggtg gcaacgaccc
     6361 cggggatctc gcccatgacg atgggtcccc cgactgcacg gatctcggtg tcctggatgg
     6421 gcccgcgacc ggcccggatg tggtaccgtt cggcggggaa gaggaggcgg tatgcaccgg
     6481 cgatgaggtc atgaggatcg agacccgcgg ggcaggcccc gccgcaccgg ccacagccga
     6541 tgcaggcgtc gtagagggcg gcgaggggcg cgagatcccc cgaggcggct gccgttaccg
     6601 ccgtcgtgat ctcccggtcg ttcgggcagg cccgcatgca gctgaaacag ccggtgcagg
     6661 tctttgcgac ctcgatgagg gaggctcggt ccggtagctt catcgaactt tttcgactcg
     6721 gggctatctc catggccacc ctgaccgcca cctccccgac cttgtccgga tcgaggatgg
     6781 ccgcccccgg ttccttcccg tcgacgaggg aacggacgat ctcctccgga tcgtccttcg
     6841 tgcggtccgc gagcccctgg agattctttg cattcgtgac gatgagaggg gcaccggtct
     6901 tcatcgcctc cctgagcacg tcggtgcgga tgcactgctc gtccaccacg atgacgtcgg
     6961 ccatgccaga gcggatgaac ctgagctgct gggacagggc gcccacgatc cgggctcccg
     7021 ggttgtaccg ggtaaggtcg agggcggtgc agcagatccc gcagacctcg atctggttct
     7081 cgacgcccat ctcccgcatg gcatcagaga tggcgatggc aggggggacg ttgtggccga
     7141 tgacgaggat gaccggctta ccgggatcaa ggcaccccgg cccgcactcc acgaggggcg
     7201 tctcggcctc ccccttcggg aaggagtagg cggagatctg ggcgaggtcg ctcccctcca
     7261 gggccacgtg gtcgatcatc cccgcgtgga ggaccttcga ttcgaggtcg agaggatctc
     7321 cctcctggcc ggtgtggcag caggagagga gctgggtgat ctccccctca cagtaggccg
     7381 cgatatcctc gagatccccg agggttttcg gcttgatgcc tgtcacgagc cgggcatggg
     7441 gggcctccag gaggaccgag gacccgacat cgatgggatg gtcccggccg aaggagccga
     7501 tgagcctgga gaggagatgg cgcatgtgcc cggcgtgggt ggcggccccc atggcacagg
     7561 cgaggaggac gatccgggac tgctgcgcgg ccatgtcgat cccgcaggcc ccccgttttc
     7621 cccccgagag gtcgcatttt cccatggtgc agagacagca gaggtcgcag aaaggaaggt
     7681 agaagggatg gtacttcgag agaagggtgc ggtcccagga ccggagggtg gcgatgccgg
     7741 gcatcggggt cgggcccggt tcctcctccc aggcctcgat gaccctcccg acgttgaact
     7801 cgacgccctt tgccctcagc cggtccgtga tgagctcgtc gatggtaacc ttcagcttca
     7861 ttcgcggata agggaggatg gagcgcctgc gcagataaag atgctgaccc agaatgggca
     7921 tgaaaaaggc tttggaaaag tatatctggg atttcggccc ggtgctcagg gaaccgggtg
     7981 ccgccggccg ctcccgtccc gggagtacat cccgaactcg ctcccgatga gccggtcacc
     8041 ccggatgacc ggtccctccc ggcatacccg gagcccggtt ggatcgatgg cacacgaccc
     8101 gcataacccg acgccgcact tcatgtaccg gtggagggag aactgtcccc ggtccgcgat
     8161 cccttcatcg tgaaggcttt tcaacaccgc ccgcatcatc ggctcgggac cgcagacaca
     8221 gatatgatcg aatgcctgga ggtcaaccct gtccaggagg accgtgacag gtccacggcg
     8281 gccctcggac ccgtcgtcgg tcgcgatcct cagggcagtc ttaccggcga gagccttcct
     8341 gaaagggaga tctttttctg ttccggcccc gaggaggaag accccgacat gcccctccat
     8401 tgcgaggggg agaaggggcg cggagccgat acctcctgct atcgcgagga ccattccctg
     8461 gattgaaaac ccctggccgt aaggtccccg gatcccgagg agatcgcctt cattcagggc
     8521 gaacatcgca gaggtcgcgt ccccaacctc ctggaccgag atggagttct tccccgagag
     8581 ggccatgggg atctcgtcca cccccggcac ccagaccatc acgaactgcc cgggggtgaa
     8641 ccggaaggat tccgagaggt ggaaggtccg gatcttcggg gtctctctca cgatccccct
     8701 gatggagacg gtccgtggaa gtccgtcatc catgggcgca ccccacgatc tcttcggtct
     8761 tctttccgtc aggggtgtag agatcccgtg cgatcttcct gaagatcccg atgtcatcca
     8821 ggacggcggt cccgatctcg acggcggccg cccccgccat catcatctcc accacgttat
     8881 ctgccgtgga gatcccgccg cacccgacga tgggaatatc gcaggcgtcg tagagctcgt
     8941 acacgcacct gaccgcgatg gggaagatcg cttttcccga gaggcccccg aagcggttcc
     9001 cgaggatcgg ccttcccatc gggaccgaga tgcgcatggc cttcaccgtg ttgatggcga
     9061 cgatggcgtc ggccccgccc cgctcggccg ccgcaccgat ctccgtgatg tcggttacgt
     9121 tcggggtgac ctttacccag gtcggcctcc cgagggcctt caccgcccgg gtgcactcct
     9181 ccacgagacc cgggtctgtc ccgagggccg acccgaaccc ctcggcatgg gggcaggaga
     9241 ggttcagctc aaaacctacg gcagagtccg cgaaccagga cgcgaccctc gcgaactccc
     9301 cgggatcccc gccgaagatg ctcacgacca cgggttcccc ccggagggcg gggagctcgc
     9361 cggcgaagtc ccgggacggg ttcgggaggc ccatggcgtt caggagcccc ccctcctgga
     9421 ccaccaggca cgggccgggg tggccctcga ggggaacggg cccgatggac ttggttacca
     9481 ccccgccggc gccctcccgg aggatcctcc cgagggaggc gccggtggtc ccgagaatcc
     9541 cggccgcgag gatgagatgg ttccggaggc cgactccccc caccgtgacg ggtccggacg
     9601 gagacctgat catccgaaga gagtctgcgt ttatttattt aataggatac ccatagtcta
     9661 ggcttgatga gtaccctcgc cgtcatcggg gtagggaaga tcgggggcga ggcggcctac
     9721 gtctctgccc tcgaggggct cgtggacgag ctcgtcctct atgacgtgaa cacccccctg
     9781 ctccaggccc aggtcctgga cctccaggac tgcggcctcg acatcggggt ctccacggac
     9841 acggccggca tcagggaggc cgacatctgt gtctacgcgg caggggtctc gcggaacccg
     9901 gcgataaaga cccgtgcgga cctcctcggt atcaacctcc ccatcgccga gacctgcctc
     9961 ccgctcctga aagggttctc cggggtcttt gtcaccgtga cgaacccgat ggacatcctg
    10021 aactacttct tcgccatgag ggccggcatc ccccgggaac ggtgcatcgg cttcggcggc
    10081 cagctggaca gcgcccggtt ccggcgcatc ctcggttcct ccgggatcag gggacccggg
    10141 tacgtgctcg gggaacacgg cgaacaccag gtccccctct tccacccgct ggggaatccc
    10201 gtggacacgg agacccggga gaagatcctc atgagcctcc ggggcgcctc catggagatc
    10261 atcaagggga agggagggac catcttcggc cccggatggc acatcacccg gctcatccga
    10321 tccatcctgc agggggagga gccgcctgtt ccctgctcct gcgtcctcga cggggagtac
    10381 gggctctcgg gatgctccct gggggtgccg acccggatcg gtaaggaggg catacgaagg
    10441 atcgaggagt ggcccctgga cccgtgggag gaggagcacc tccgggaggc cggggcattc
    10501 ctcgccgatc tctcccgggg gctccatggg tgagggagga tccctgggcg cactcccggg
    10561 tttcgagaag cggaccgttg cccggaccct ttccgatttc tcggggatcc gcatcgccat
    10621 caaccaggtg gagtacacga acagccccca gggccccctc atccacatct tcggccggga
    10681 cgggaacggg tccgcggtcc acctcgaggt aacgggtttc cggccctact tctacgcgcc
    10741 cttcgaggag gtcgccggcg gcccgctccc ctccgggctc gagatcgaga ccgaccgtga
    10801 ataccgttcg atccggggcg acaccctgtc ccgcatgtac acgcgccggc cgacagatgt
    10861 ccgggatttc cgtgagcggt tccaccactt cgaggccgat atcccctttg ccacccggtt
    10921 cctcatcgac accggcctca ccggtggggt ggaggcccct tcggtccaga ctgactaccg
    10981 ttccctgcgc cctgccgagg tgaatgcccc tgcccgggtc tgcatcttcg acatcgagtg
    11041 cgaggacgag agaggcctcc ccgagccgga ccgggacaag attatcgccg ttacctgctg
    11101 ggactccttc caggacgatt acacgagttt cgttttccct gccggtggag gggtacccct
    11161 tgctccgcag gccggcgaga agatcacggt cgaggtgtac cgggaggaga aggaaatgct
    11221 cctcgccctc gcccggtacc tgaaagagcg ggaccccgac gtcctctcgg gctggaacat
    11281 cctcgagttc gacatcccct acatcatcgc ccggatggag atcctcgggc tctccccgac
    11341 cctgcttgcc cgtctcgagg aacggaccga gcgggttgcg ctccggggaa gggccctctt
    11401 cgatctcctg gacgggtaca agaagatgca cgggagccag cgggagtcct accggctgga
    11461 ttccgtcgcc gaggaagagg tcggggagac gaagatccgt ttcacgggca ccctctcgtc
    11521 cctctggagg gaggaccccg gccagctcgt cgactacaac cggaaggacg tggagctttg
    11581 cgtggccatc aaccggaaga atcacatcgt cgagttctac cgggagatcg cccgctacgt
    11641 cggctgcccc ctggaccgga ccctcaactc ctcttcggtc atcgacatct acatcctcag
    11701 gaaggcatcg ggccggttcg tgctcccctc aaagggcttc gctgccgccg aggagttcga
    11761 gggggcgacg gtcttcgacc ccacccgggg cgtgaaggag aacgtcgtcg ttctcgacct
    11821 gaagtccctg taccccatgg ccatgatgac catcaatgcc tcgccggaga ccaaggaccc
    11881 cgacggggag taccgggcac cgaacggagt ccggttccgg cgggagcccg atggcctcac
    11941 ccggtccatc atcgccgagc tcctgaagga gcgggagcag aagaagaagc tccgcgacgg
    12001 gtaccccttc ggctcccccg agttcgtcct ctacgacctc cagcagaacg tcctcaaggt
    12061 catcatgaac acctactacg gggtctcggg gtatgcccgg ttccggctct atgaccggga
    12121 gatcggatcg gcggtgacct cggtggggag ggccatcatc gagcatacga ggacggtcat
    12181 cgaggggatg ggctaccatg tcatctatgg ggacacggac tcctgcatgg tccagatccc
    12241 gtccatggag accgccgata ccatcgcggc cgcccggaag atcgaggcgg cggtgaacgc
    12301 gagctatcgg gagttcgcga aaaagacgct caacgccgac tcccactact tctccatcaa
    12361 gttcgagaag atctaccgcc ggttcttcca ggcggggaag aagaagcgct acgccgggca
    12421 cctcgtctgg aaggagggga aggacgtgga cgagatcgac atcgtcggct tcgagctccg
    12481 ccggtccgac tacccccaga tcacgaagga ggtccagggg aaggtcatcg ggatgatcct
    12541 gaagggcgag gggttccccg aagtgaaggc gtacctgggt gagatcatca aaaacttccg
    12601 ggcaggaaag tacagcttgg acgaggtggg catccccggc gggatccaga aggccct
//
LOCUS       DATGDI010000255        16039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_318938, whole genome shotgun sequence.
ACCESSION   DATGDI010000255 DATGDI010000000
VERSION     DATGDI010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 16039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16039
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318938"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2023)
                     /locus_tag="VKO45_09570"
     CDS             complement(<1..2023)
                     /locus_tag="VKO45_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HMA06158.1"
                     /translation="MDGTAWALTLTVAVPFIGAAAILALRNRPDEREAASLVTAVATF
                     LLAASALPPLFASGGAMTPSLTVLPGFTIQLHGDALGLLFATLASFLWILTTVYSIGY
                     MRGLKEHAQTRYYASFALVIGATMGVALSANLFSLFIFYEILTVATYPLVVHRETEEA
                     FAAGRKYLVYTLSGGVAILAGTFLLAGMGASLDFVAGGAPSVAALAPAFARLAFLLLF
                     AGFAVKASIVPLHGWLPSAMIAPTPVSGLLHAVAVVKAGVFGILRLLLYLYGPDLVGS
                     LGLQNLVIGAAVLTIIVGSLFALLQDDFKLRLAYSTVSQLSYIVLGAAILLPGGLTGV
                     TDAGRAAAILGAGFAIAAHAFGKLAMFFVAGAVAVETGKTKISELDGLGRRMPAEFGA
                     FTLAVLSMAGLPPMAGFVSKWYLSVGAWSAGDWWILLVFAASSVLNLAYFLPIIIRAF
                     FPAYQGSGSGARPTLALPLLATAGCGLLFGLFPSLPYGPFTIAARMAADVTGTAMMSF
                     GSLSPSLAVPPFLIFLIGGPLVLALRGRARQAGLVTVAGIALLDVLFLPGGYLGASAA
                     GGVSLWQVPFMGYTLTLLRVDALSSLAGTIFALITFLAVLYAASIDRPRLHLFALLYA
                     GASMGAVFAGDWITLLVFWELMAIISTLLIWEEGGEAIGAGFRYFLFHGL"
     gene            complement(2023..2340)
                     /locus_tag="VKO45_09575"
     CDS             complement(2023..2340)
                     /locus_tag="VKO45_09575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HMA06159.1"
                     /translation="MNITSAILVALAASLFAVGLAGLIGRGNLIRMVIGLEFLGKGAS
                     LLLITAGYAAGDTGVSQAVVFTLIAIEAVVAAVALALVILVKRTTGSLDSAMQGISGR
                     GAS"
     gene            complement(2337..2579)
                     /locus_tag="VKO45_09580"
     CDS             complement(2337..2579)
                     /locus_tag="VKO45_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06160.1"
                     /translation="MRGATAAGVLTGILFLLVILAAVLPGAPALISAGPGLEGVGAAL
                     WVGRAAEVLLQGFILLAGAAAVLLFLGTRAPKEGPP"
     gene            complement(2576..2836)
                     /locus_tag="VKO45_09585"
     CDS             complement(2576..2836)
                     /locus_tag="VKO45_09585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HMA06161.1"
                     /translation="MVSDPLIFAFLGLAAVCMAILSFTLRNLVRAVVAFALSSALLAG
                     IFYLLASPYAAALELTVGAGLVTVLFLVAILLAGGEELEAPS"
     gene            3140..5221
                     /locus_tag="VKO45_09590"
     CDS             3140..5221
                     /locus_tag="VKO45_09590"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HMA06162.1"
                     /translation="MYIHLIITVVVSFIALAVAALFAREVLEADTGTPKMQEVAGAIR
                     EGAEAFIKRQYSTIAILGVVVAILLFLVYVIAGDLDTAWHTAVAFIFGAFCSGLAGII
                     GMWIAVRANLRSAAAARTSAGKAIVLALRGGAVSGLTIVALSLLGVSLIFTAFGSNPQ
                     ETPFLIVGFGFGASFIALFAQLGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIAD
                     LVGDNVGDCAGRGADLFESTAAENIGAMILGVALFPYFGVNGILFPLVARALGLIATI
                     VGILAVTEKGAEKGSPMDALNRGYYICAALATLFFLGASYYMFGPTYYLWFFAAGVVG
                     IVLSIFFVYITLYYTDYTHRPVLEIVKASVTGPATNIIAGFSVALECTAIPAVAIGLA
                     LILSYSLGVWSGIPNGGFYGTAIATMGMLATCAYILAMDTFGPIVDNAGGIVEMSGES
                     EETQKKIARLDAAGNTTKALTKGYAVGSAALSAFLLFSAYMDKVTEIVSKTPGMANYV
                     FNVVNMANPTVFVGALLGAMLVFLFSGLAIRAVGETAQYIILEVRRQFQDKGILEGTK
                     KPDYASCVDITTRGALKSMVIPGIIAVAFPVSVGIVLRHEAMAAFLMVATIVGILLAL
                     VLNNGGGAWDNAKKFIETGQFGGKKSEAHKAAVVGDTVGDPFKDTAGPSLHVLVKLLA
                     TTTLVLAALYI"
     gene            5552..6097
                     /locus_tag="VKO45_09595"
     CDS             5552..6097
                     /locus_tag="VKO45_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06163.1"
                     /translation="MTEPALNTLGLQKPLMAVTGILFLVIVLPVVIGWTAGSSPAQVI
                     GFIGSIFLLQGLAPTVGIGLGLPPLQLFGILTSVATGIILGIFMVCDLLSGKSERITN
                     WIRKIEATMEKYQFLHTLGEYMLIPIMWVPGIGLYGTPVIAWVLGWRSLRAVLLMLAG
                     WLIACLSVAGMVKGVLHFIGL"
     gene            6467..6811
                     /locus_tag="VKO45_09600"
     CDS             6467..6811
                     /locus_tag="VKO45_09600"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 C-terminal domain-containing protein"
                     /protein_id="HMA06164.1"
                     /translation="MSNESANPMIDAGVAGAEHVKLQAAEALEEAARKLRNVDVSAKG
                     EDIRRILEDVEGRINRTRAEVEAGYEGLDTEFHRRVEPVETLIADHPLPAVLIAAAVG
                     ALIGMLISRAKD"
     gene            6822..7196
                     /locus_tag="VKO45_09605"
     CDS             6822..7196
                     /locus_tag="VKO45_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06165.1"
                     /translation="MDPAGCEEELTVEAEFVRLVRYIDLYLQQKTDLFLQRYVFEPLG
                     FLAVRVMALVVIVTLLAAGTVVLVVGLVFLLATLVPLWAALIITAAIMLIAGGISGYR
                     LVSQRIVLKTPGAREVMNRGRT"
     gene            7183..7578
                     /locus_tag="VKO45_09610"
     CDS             7183..7578
                     /locus_tag="VKO45_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06166.1"
                     /translation="MEEHRRVTEEDLLMTEAMIAESYGRLKMSLERTPSRVLRSASRT
                     IVEHPIAAAGAVTVGGILAYKVLGMLQPRIADEKNGMGRKAGSRPNLAGEIMSAVLPL
                     ATPYIARFIGDSLGSMLAGGGQTRDRRSR"
     gene            7889..8314
                     /locus_tag="VKO45_09615"
     CDS             7889..8314
                     /locus_tag="VKO45_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06167.1"
                     /translation="MLSSKIAVNRERVARENNNLMKTGELEFIWIMKKVYVEEPRAVA
                     EIIRGYTNLEIVSLSFLIASAANIIDMRACDEDISRTQVLHSLGFRAEDPERYDENEI
                     HHGEENELLFSQGHIEESHVQKYPFSFPLYKVFSLPSSK"
     gene            complement(8391..9053)
                     /locus_tag="VKO45_09620"
     CDS             complement(8391..9053)
                     /locus_tag="VKO45_09620"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMA06168.1"
                     /translation="MDREYEPMLPIGDEEASRRLFPAVTYTLIALNVLVFFLELINGD
                     PFVQQWSFIPSRFLANPAGDFVTIFTSMFMHAGWLHIGGNMLYLWIFGDNVEDWFGHL
                     PYLGFYLLCGVAATFAQLAFSMGSTIPSLGASGAIAGVLGAYIVLFPEERITVLFDYG
                     GVGRVPAIIVIGLWFILQLFSGIGSVAATTDTGGVAYMAHVGGFVMGVLLTVLSGGKR
                     QD"
     gene            complement(9093..9668)
                     /locus_tag="VKO45_09625"
     CDS             complement(9093..9668)
                     /locus_tag="VKO45_09625"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HMA06169.1"
                     /translation="MSETEVLEAPMIWQSFVLRGILALITGFLVLLWPGIAVLAVVIL
                     FGVLLLVASIQTLYFASKVPKGLPQPVVPIVTAVIGLVLGIVALVFPWITAVALTWLI
                     AALMMWMGLIEIASAVFHPEFMKHPVLFGLSGAVGVVLGGIFLFYPGLGAQVLVMVYF
                     GFFALLYGILSIAIGLQVKGEEKVIEKKAAA"
     gene            9926..10717
                     /locus_tag="VKO45_09630"
     CDS             9926..10717
                     /locus_tag="VKO45_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HMA06170.1"
                     /translation="MAGSLKRLTLVFLVLMVLAAGTLLFYGSFIREPTNLPGFLDISI
                     KIAIIGAFWLANLFLLRRVMPTIKENVGEQAATTIETILVVIAGIVLIFALLSTLGIS
                     PENLLVGAGIASITMGVIVSSFMSNVLSGILVVAAHKLRPGDEVIVCDTDGTITEITA
                     MVTRVRTEKGDIALSNSGIASGLVVVTKIHSHEMAAHGKLPYSEGDRIVINYVEGEGT
                     VTEITPFHTTLLLDSGEELAFLNSSVLTGEVAVARITRRKPVLQK"
     gene            11274..11852
                     /locus_tag="VKO45_09635"
                     /pseudo
     CDS             11274..11852
                     /locus_tag="VKO45_09635"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
     gene            complement(12183..12767)
                     /locus_tag="VKO45_09640"
     CDS             complement(12183..12767)
                     /locus_tag="VKO45_09640"
                     /inference="COORDINATES: protein motif:HMM:NF015317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HMA06171.1"
                     /translation="MPDSSPSGIDRVERLNERPVLGIMYRTSRFIVELGITALLLAAV
                     IVVIFSFTSLFQIVSHLARGGFVFEEMATYLAVSMTEIIDLTLLGIVLIIIALGVYQL
                     FIDPTLELPKWLETHRIETLEERLLAVVVVLLPVLFVGHVATWDGGIGIIGLGIAIAS
                     VMAAIMYVISTMARTMKTVADLEAQPPDVKEKMS"
     gene            complement(12818..13372)
                     /locus_tag="VKO45_09645"
     CDS             complement(12818..13372)
                     /locus_tag="VKO45_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06172.1"
                     /translation="MKEKRRKTILRTIRLSEEIDHLLERDAQEQNISTNALIGKIMTR
                     YMEWDRVVEKLDFVSFSNAFFKALLNEVSDERIQEMASQEAIRQIKNQAMWDFGKADF
                     DTLLKTIILRGKYGIASHVSVESRGEGDCVITLHHNWGPKGVVFFRSFFDSFIRGELR
                     TQPTIDFGDDVLIVSFHTPLKRTP"
     gene            complement(13612..15009)
                     /locus_tag="VKO45_09650"
     CDS             complement(13612..15009)
                     /locus_tag="VKO45_09650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HMA06173.1"
                     /translation="MLGDGEIVGRFLEAKLQVHPDVVSYLRERNDPALVERVISLVPS
                     GTLVVNPAHIPDLSPVKDGERFLPEPELEVIAGKPGDSSEKNRFEDFFPYFRDRYTRL
                     SEILRSRVNPVPIEALTRSTRYRQEECMVIGMVLDVRTTANGHRLVDLEDPTGQIPVL
                     FNKGRDGFADAERLLPDEVIGARGRLSQDGRLLFAEQLVRPDVPLTHAPFTSSRGGKA
                     VLISDIHAGSTTFLEDAWERFIEWLPSSGAAYLLICGDLVDGIGVYPNQEEELAIRDI
                     YEQYLKLGELMAGIPRGIAVVISPGNHDVVRPSEPQVAIPQEFRKGFPENCRFVENPA
                     LLSLEGVRVLMYHGRSIDDMIGLIPRASYSRPEEMMVEMLERRHLAPVYGRRTPLSPE
                     PKDRLLIDPVPEILHTGHVHTSGITRYRGVLGVNAGCWQAQTKFQKQVNITPTPGRAV
                     LVNLRTLEPKVMDFA"
     gene            15182..15880
                     /locus_tag="VKO45_09655"
     CDS             15182..15880
                     /locus_tag="VKO45_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HMA06174.1"
                     /translation="MAGETILGRVKGAWEGFRRSENPVVAFAREILWVVAVVGGIALL
                     LFLVCGTWPAVVAVESESMLPNMHVNDLVFVVEKDRFGQLQTYETGLSAGYQKFGNFG
                     DVIIYHPNGAADIHPIIHRAMAYHNASWFGINIGVKATHDGYLTKGDNNRIPDQESAI
                     QGFGPIQPVEPDWIVGKALFSVPLLGYPTLHYPEFAAVVIILLVIHEIWASRSEKEEE
                     KGKKGRKTGKKGRR"
BASE COUNT         3464 a         4559 c         5005 g         3011 t
ORIGIN      
        1 ccaggccatg gaagaggaag taccggaatc ctgccccgat ggcctcaccg ccctcctccc
       61 agataagcag ggtggagatg atggccatga gctcccagaa gacgaggagg gtgatccagt
      121 ccccggcgaa gaccgccccc atcgaggcgc cggcatacag gagggcgaag aggtggagcc
      181 gggggcgatc gatggatgca gcatagagga cggcgaggaa ggtgatgagg gcaaagatgg
      241 tcccggcgag ggaggagagg gcgtcgaccc gaaggagggt cagggtgtag cccatgaagg
      301 ggacctgcca gagggagacc ccgcccgccg cggatgcacc gaggtagcct cccgggagga
      361 agaggacgtc gaggagggcg atgcctgcga cggtgaccag gccggcctgc cgggccctcc
      421 cccggagggc gagcaccagg ggcccgccga tgaggaagat gaggaagggc gggacggcga
      481 gggagggcga aaggctcccg aagctcatca tcgcggtgcc agtcacatcg gccgccatcc
      541 gggccgcgat ggtgaagggg ccgtagggaa gggaggggaa gagcccgaag aggagcccgc
      601 accccgccgt cgcgaggagg gggagggcga gggtcggccg ggcccccgat cccgatccct
      661 ggtacgccgg gaagaaggcg cggatgatga tggggaggaa gtaggcgagg ttcagcacgc
      721 tcgaggccgc aaagacgagg aggatccacc agtcacccgc gctccaggcc ccgaccgaga
      781 ggtaccactt cgagacaaaa ccggccatgg gggggagccc ggccatcgag aggacggcaa
      841 gggtgaaggc gccgaactcg gcgggcatcc tccgcccgag accgtcgagc tccgagatct
      901 ttgtctttcc ggtctccacg gccacggccc ccgcgacgaa gaacatggcg agcttgccga
      961 aggcatgggc ggcgatggcg aagcctgcgc cgaggatggc cgcggcccgc ccggcgtccg
     1021 tgaccccggt caggcccccc gggaggagga tggcggcgcc gaggacgatg tacgagagct
     1081 ggctcaccgt ggaataggcg agtctgagct tgaagtcgtc ctgaaggagc gcgaaaaggg
     1141 agccgacgat gatggtcagg accgcggcgc cgatgaccag gttctggagc ccgaggctcc
     1201 cgacgagatc cgggccgtag aggtagagga ggaggcggag gatcccgaag accccggcct
     1261 tcaccaccgc gacggcgtgc aggagcccgc tcaccggggt gggggcgatc atcgcggagg
     1321 ggagccaccc gtggagcggg acgatggagg ccttcacggc gaagccggcg aagaggagga
     1381 ggaaggcgag ccgggcgaag gccggtgcga gggcggcgac ggagggtgcc ccgccggcga
     1441 cgaagtcgag ggaggccccc attcccgcga ggaggaaggt ccctgcaagg atcgcaactc
     1501 cgccggagag ggtgtagacc aggtacttcc tgcccgcggc aaaggcctcc tcggtctccc
     1561 tgtgcaccac cagggggtac gtggcgacgg tgaggatctc gtagaagatg aagagggaga
     1621 agaggtttgc cgagagggcg acccccatgg tcgcaccgat gacgagggcg aaggaggcat
     1681 agtaccgggt ctgggcatgc tccttgagac cccgcatgta gccgatggag tagacggtgg
     1741 tgaggatcca gaggaaggac gcgagagtgg cgaagaggag gccgagggcg tccccgtgga
     1801 gctggatggt gaaccccggg aggacggtga gggagggggt catcgcaccg cccgatgcga
     1861 agaggggggg gagggcggat gccgcgagga ggaaggttgc cacggcggta acgagggagg
     1921 ccgcctctct ttcatcgggc cggttcctga gggcgaggat ggccgcggcc ccgatgaagg
     1981 ggacagcgac ggtgagggta agggcccagg cggtcccgtc catcaggatg cccccctgcc
     2041 ggagatgccc tgcatggcgg agtccaggct ccccgttgtc cgcttcacca ggatgacgag
     2101 ggcgagggcg accgcggcca cgaccgcctc gatggcgatc agggtgaaga ccaccgcctg
     2161 ggagaccccg gtgtcaccgg cggcgtatcc cgcggtaatc aggaggaggc tcgcgccctt
     2221 ccccaggaac tcgaggccga tgaccatccg gatgaggttg cccctcccga tgaggccggc
     2281 gaggccgacc gcaaagaggg aggccgccag ggcgacgagg atggcggagg tgatgttcac
     2341 gggggtccct ccttcggtgc ccgggtaccg aggaagagga ggaccgcggc cgccccggcg
     2401 aggaggatga acccctggag gaggacctcg gccgcccgcc cgacccagag tgcggcaccg
     2461 acgccctcga ggccgggtcc ggcggagatg agggcggggg ctcccgggag gaccgccgcg
     2521 aggatgacca ggaggaagag gatgccggtg aggacgccgg cagcggtggc tcccctcatg
     2581 acggggcctc cagctcctcc cctcccgcga ggagaatggc cacgaggaac aggaccgtga
     2641 ccaggccggc ccccacggtc agctcgaggg cggccgcgta cggggaggcg agcaggtaga
     2701 agatgcccgc gagaagggcg ctcgagaggg cgaaggcgac gactgcccgg accaggttcc
     2761 tgagggtgaa gctgagaatg gccatgcaca ccgcggcgag ccccaggaag gcaaagataa
     2821 gcggatcaga caccataatc ccgacacctc ctccgccggc gtgcccgaga tgcccgcagg
     2881 caggaaacct caagggatgc agtgtacgtt ccgggaggga gcttaaattt gttattattt
     2941 atcatgaaaa atctcgggcg gcccatcgga atgcaggggc cggcgcttac ctatccggga
     3001 acatgccggg cctttttccc ggaatctggc atgattctcc cttcccgccg ggggaagaat
     3061 ggataccctt aaaccatcca aggaaccact acattccaca tccgttgagc ggatactccg
     3121 aaaaacgagg tgaagggaca tgtatattca tctgatcatc accgttgtcg tgagcttcat
     3181 tgctctcgca gtggcggccc tctttgcacg ggaggtgctg gaggccgata cgggaacccc
     3241 gaagatgcag gaggttgccg gggccatccg ggaaggcgca gaggccttca tcaagcggca
     3301 gtattcgacc atcgccatcc tcggagtggt cgtcgccatc ctgctcttcc tggtctacgt
     3361 tatcgcagga gatctcgata ctgcgtggca cacggcggtg gcgttcatct tcggcgcctt
     3421 ctgttcaggg ctcgcgggga tcatcgggat gtggatcgcg gtccgggcca atctccgttc
     3481 cgcggcggca gcgaggacga gtgccggaaa ggccatcgtc ctcgccctca ggggcggggc
     3541 ggtctccggc ctcaccatcg tggcgctctc cctcctcggg gtctccctta tcttcacggc
     3601 ctttggctcg aacccccagg agacaccgtt cctcatcgtc gggttcggct tcggcgcctc
     3661 cttcatcgcc ctctttgccc agctgggcgg cgggatctat accaaggccg ccgatgttgg
     3721 cgccgacctc gtgggaaagg tcgaggcggg gatccccgag gacgaccccc ggaaccccgc
     3781 ggtcatcgca gacctcgtcg gcgataacgt cggggactgc gccgggcgtg gcgccgacct
     3841 cttcgagtcc accgctgccg agaacatcgg ggccatgatc ctcggtgtcg cccttttccc
     3901 gtacttcggg gtgaacggga tcctcttccc cctggtcgcc cgtgcgctcg gtcttatcgc
     3961 gaccatcgtc gggatcctcg cggtcaccga gaagggggcc gagaagggga gcccgatgga
     4021 tgccctgaac cgggggtact acatctgcgc ggcgcttgcg accctcttct tcctgggagc
     4081 atcctactac atgttcggcc ccacgtacta cctgtggttc ttcgctgcag gggtcgtggg
     4141 aatcgtgctc tccatcttct ttgtgtacat taccctctac tacacggact acactcaccg
     4201 gccggtcctc gagatcgtca aggcgtcggt gacgggcccg gccacgaaca tcatcgcagg
     4261 tttttccgtc gcccttgagt gcacggcgat ccccgccgtc gccatcgggt tagccctcat
     4321 cctctcctat tccctcggcg tctggagcgg gatcccgaac ggggggttct acgggacggc
     4381 catcgcgacg atggggatgc tcgccacctg cgcgtacatc ctcgcgatgg acaccttcgg
     4441 ccccatcgtg gacaacgccg ggggaatcgt cgagatgagc ggggaatcgg aggagaccca
     4501 gaagaagatc gcccggctcg atgcggcggg gaacacgacg aaggccctca cgaaggggta
     4561 tgccgtgggc tccgcggccc tctcggcgtt cctgctcttc tcggcgtaca tggacaaggt
     4621 gaccgagatc gtgtcgaaga ccccggggat ggcgaactac gtgtttaatg tcgtcaacat
     4681 ggcgaacccc acggtctttg tcggtgccct cctcggggca atgctcgtct tcctcttctc
     4741 gggcctcgcc ataagggccg ttggggagac ggcccagtac atcatcctcg aagtgcggcg
     4801 ccagttccag gacaagggga tcctcgaggg gacaaagaag ccggactatg catcctgcgt
     4861 ggacatcacg acccgggggg ccctgaagag catggtcatc ccgggcatta tcgcggtcgc
     4921 gttcccggtc tccgtcggta tcgtgctcag gcacgaggcc atggcggcct tcctcatggt
     4981 ggcgaccatc gtggggatcc tccttgcact cgtcctgaac aacggcggtg gtgcctggga
     5041 caatgccaag aagttcatcg agaccggcca gtttggcggc aagaagtccg aggcacacaa
     5101 ggcggcggtt gtcggcgaca ccgtcggcga cccgttcaaa gatacggcgg gtccctcgct
     5161 ccacgtcctc gtcaagctcc tcgcgacgac aaccctcgta ctcgcggcgc tgtatatctg
     5221 atcccatcct tctctttttc ctccgaacct ggttcacccg gtctatctcg gctatccgtt
     5281 ccaagtggat ccttctgggc caatcactgg ttttttctcc tggttcggtc cgcggtcccc
     5341 tcggcatggt ttaccatccc ggcaggcctg ccaagcagct ggaggagagg gcaaggactc
     5401 agccaacacc ggatttgatc aggtgggact accctcagcc ctgccaggga gagctaatga
     5461 agcggctaat gaagcggatc cttccgattc cggtagggga catcgcgacg ctcgggaagt
     5521 tttatcaatt ccgggacaca aggcaccccg tatgactgag cccgcactga acaccctggg
     5581 tctccagaaa cccctcatgg ccgtaaccgg gatccttttc ctggtcattg tcctccccgt
     5641 ggtcatcggg tggacggccg gctcatcgcc tgcccaggtc atcgggttca tcggatccat
     5701 ctttctcctc cagggacttg ccccgacggt gggtatcggc ctcgggctgc cgcccctgca
     5761 gctcttcggt atcctgacct cggttgccac ggggatcatc ctcggcatct tcatggtctg
     5821 cgatctcctg tcagggaaat cggaacggat taccaactgg atcaggaaga tcgaggccac
     5881 catggaaaag taccagttcc tgcacaccct cggggagtac atgctgattc ccatcatgtg
     5941 ggtcccgggc atcggcctct acgggacccc cgtcattgcc tgggtcctgg gctggagaag
     6001 cctgcgggca gtcctcctca tgctcgcggg ctggctcatc gcctgcttaa gcgttgccgg
     6061 gatggtcaag ggggtcctcc atttcatagg cctgtgaacc ctgccgtaca tttcatcttt
     6121 atgatggggc gactcccaga tcacaatccc catgcctcgt atccggaaat ccaggcgagg
     6181 tgataaagtt caggagatga ttggcgaaac ctcgggcgtt gtgaggattt cctggtctat
     6241 ctctccgagg gagacgattt ttccgccata tgcgattata tcagcaaacg tgtcggattt
     6301 catgtcccga tccttccggc accgcagcac cagctctgcg aggtcacgaa ttgcataaac
     6361 ctaaataaat cctggaatgt atattaacaa cctctggtat ggatccgggg tggaaccttt
     6421 gtaaggcccc tgaaccatgt cagggaggca gtaacggtgt atattcatgt cgaatgaaag
     6481 tgcaaacccg atgatcgatg caggagttgc cggcgcagag catgtgaagc tccaggcggc
     6541 cgaggccctc gaggaggccg cccgaaagct gcggaatgtg gacgtatcgg caaagggtga
     6601 ggatatcagg cgcattctcg aggatgtcga gggtcgaatc aaccggacca gggccgaggt
     6661 cgaggccggg tacgaggggc ttgacactga gttccaccga agggtggaac ctgtcgaaac
     6721 tctcatcgcc gatcaccccc taccggcggt cctcatcgcc gccgcggtag gggccctcat
     6781 cgggatgctc atctcccggg caaaggattg aagggaatcc catggatccg gctggctgtg
     6841 aggaagaact gacggtggaa gccgagttcg tgcggctcgt caggtacatc gatctctacc
     6901 tgcagcagaa aacggacctg ttcctgcagc ggtacgtgtt cgaacccctc ggtttcctgg
     6961 ccgtcagggt catggccctt gtggtcattg tcaccctcct tgccgcaggg acagtagtcc
     7021 tcgtggtcgg cctcgtgttc cttctcgcga cccttgtgcc tctctgggcc gccctgatca
     7081 tcaccgccgc catcatgctt atcgccggag gaatttccgg gtacaggctc gtatcacaga
     7141 ggattgtcct taagaccccg ggcgcccggg aggtgatgaa ccgtggaaga acataggcgc
     7201 gttaccgagg aggatctcct catgaccgag gccatgatcg ccgagtctta cggcaggttg
     7261 aaaatgtccc ttgaacggac accttcccgg gtcctccggt ccgcgagccg gaccatcgtc
     7321 gagcacccca tcgctgccgc cggagcggtg accgtgggag ggatactcgc gtacaaggtt
     7381 ctcgggatgc ttcaaccgcg tatcgccgac gagaaaaacg gtatggggag gaaggccggg
     7441 agcagaccga accttgcggg ggagatcatg tccgcggttc ttcccctcgc caccccgtat
     7501 atcgcgaggt ttattgggga ttccctgggc agcatgctcg ccggcggtgg ccaaacccgg
     7561 gacagacgta gccggtaaga accacttttt ttcccgaccc atcgacctcc ctggtggcgg
     7621 cggaaaaatg gacacaacgt ttaattccct gtactccggt attacccgtc tcttcaacct
     7681 ggactgcatc cccggaggag tccttgagct ggtaagagcg acgcgataac ggattccctc
     7741 ccggtcatcc gctgctccga atgccccatc cttcgccggg agaggggcag gaggtgaggg
     7801 gatcgactcc cctcccctcc ccgccgagga gttcctggct tatcccggtt ttggtatttt
     7861 cacccggata tacaatcgat aggccaggat gttgagttca aagattgccg taaacaggga
     7921 aagggttgcg agggaaaata acaacctgat gaaaaccggt gagctggaat tcatctggat
     7981 catgaagaag gtgtacgtgg aagagccaag agcagtcgcc gagataatca gggggtatac
     8041 caatctagag atagtttccc tgtcattctt aatcgccagt gcagcgaata taatcgacat
     8101 gagggcttgc gatgaggata tatcgaggac acaagttctc cattccctgg ggtttcgggc
     8161 cgaagaccca gagaggtacg atgagaacga gatacatcac ggcgaggaaa atgagctcct
     8221 gttttctcag gggcacatcg aagaatcgca tgttcagaag tatccttttt cattccccct
     8281 ctataaggtg ttttctttgc catcttcgaa ataagagggg catcagccct gctcgggacg
     8341 acgcggggct ggaggggagg gggaaggtgt atccggcagg atagcacatg ctagtcctgc
     8401 cttttccccc ctgaaagaac cgtaagcagt actcccatca cgaatccacc gacatgtgcc
     8461 atgtaggcaa cgccgcccgt gtctgttgtg gcagcaacgg aaccaattcc gctgaacagc
     8521 tgcaggataa accagagccc gataacaata attgcgggta cccggcccac tccaccgtag
     8581 tcgaacagca ccgtgatcct ctcctcgggg aacaacacga tgtatgctcc gagcacaccg
     8641 gcaatcgctc ctgaagcccc caaggagggt atggtcgatc ccatgctgaa cgccagttga
     8701 gcaaacgttg ccgcaacacc gcagagaagg tagaatccca ggtaaggcag gtgcccgaac
     8761 cagtcttcca cgttatcgcc aaagatccac aggtacagca tattgccccc gatgtggagc
     8821 cagccggcat gcatgaacat ggacgtaaag atcgtcacga aatcgccggc aggatttgca
     8881 aggaagcggc tgggaataaa tgaccattgc tgaacaaagg gatccccgtt gatcaattcc
     8941 aggaagaaga ccagaacatt cagggcaatg agggtgtacg tgaccgcggg aaacagcctg
     9001 cgagaggcct cttcatcacc gatgggtaac ataggttcgt actcccgatc catcgaattc
     9061 ttcttcttaa aaaaaaggga gagaattgcg tttcaggccg ctgccttctt ctctatcacc
     9121 ttctcctcgc ccttcacctg gaggccgatg gcgatggaga ggatgccata gaggagggcg
     9181 aagaacccga agtacaccat aacgaggacc tgggcgccga gtcccgggta gaagaggaaa
     9241 atccccccga ggacgacacc gacggctccc gagaggccga agaggaccgg gtgcttcatg
     9301 aactccgggt ggaagactgc cgacgcgatc tcgatgagac ccatccacat catgagggcc
     9361 gcgatgagcc aggtcagggc cactgccgtg atccagggga acacgagtgc gacaatcccg
     9421 aggaccaggc cgatcaccgc cgtcacgatg gggactaccg gctgcggaag gcctttcggg
     9481 accttggagg cgaagtagag agtctggatc gaggcgacga ggaggagaac cccgaagagg
     9541 atcaccactg ccagcactgc aatccctggc cacagaagga ccagaaagcc ggtaatcagg
     9601 gcaagaatcc ccctgaggac gaacgactgc catatcatag gggcttcaag aacttccgtt
     9661 tccgacataa cacatcaccg accctttctt ccaccatcat ggtatattaa ttttaccctt
     9721 tcttgcgata agcctgtccc caagacccgg cgtcccggat gcttcggtgt catcgaccat
     9781 aatggtcatt tctcatcgcc ggagcaggga atagtgtaca aagacggtga ttgtaaacgt
     9841 tctcgatata agactccggg gacaacccaa agatcctatg ccgggaacag gggcatgaaa
     9901 cattggatct tcaggtgtaa ctcacatggc tggctcccta aagagactca cactggtgtt
     9961 cctcgttctc atggtcctcg cagcaggaac actgcttttc tatggttctt tcatacggga
    10021 accgaccaat ctgcccggat tcctggatat atccatcaaa atagcaatta taggagcatt
    10081 ctggcttgca aatctgttcc tcctgcgacg cgtcatgcca acaatcaagg aaaatgtcgg
    10141 agaacaggca gcgaccacga tcgagacgat tctcgttgtc attgcaggca tagttctgat
    10201 tttcgccctg ctcagcacgt tgggaatctc tcctgaaaac cttctggtag gcgccggaat
    10261 tgcctctatc accatgggcg tcatcgtgtc ctccttcatg agcaacgtcc tctccggaat
    10321 attggttgtc gcagctcaca aactgagacc gggcgacgag gtaatcgtgt gcgatacgga
    10381 cggaaccatc accgagataa ccgccatggt cacgcgggta agaacagaaa aaggtgatat
    10441 cgcactctcc aacagcggga tagcctcagg tctcgttgtg gtcacgaaaa ttcattccca
    10501 tgagatggct gcacacggga aacttcctta ctcggaagga gacagaatag tcatcaatta
    10561 cgtcgagggt gaaggaacgg tgacagaaat aactcctttc catacgacac tcctgttgga
    10621 ctctggagag gaactggctt ttctgaacag cagtgtcctc acaggagagg tggcggttgc
    10681 caggatcacc cggcggaagc ctgtcctgca gaaataattt tcagggacca tgagagctcc
    10741 tgcccgccgc caggcagttg agcgaagcag aaaccggttc cactattccc tgccatgcat
    10801 gagaacgtgt acaaagtgta caagacccta ttttgtaaac gtttcaaata ttgccaaagc
    10861 tcgtaaaggt aaataacgtg cgcgtggcgc atgagaatgc gcaggagaga gaagctggat
    10921 atgactaaaa aattaacgcc tggaagggtt tccccggaaa atcatccgta ttttcttggg
    10981 gaggctgccg atccgatcgg ctttttgtca ggcgagttat attcggtatt ttacttcatg
    11041 aaatcatgcg cctgtgcagg aaatgatcgg aggttgatgg tatggagaaa atgttagtcg
    11101 ttgtttttga cagcgaaccg aaggcatacg aaggaacgaa gacccttcag caactcgaca
    11161 gggacggaag cattacggtc tatgccgagg cagtgatcag gaagaacccc gatgggacgg
    11221 cagcttccct gaaaatcgac gaggattttc cggttggtac cctcggaggt acggcgatcg
    11281 gcagccttat cgggcttctc ggaggcccat tgggcgtcgt ggtcggcgca acgtcgggta
    11341 cgttactggg tgcgataggt gacctgtacg tatccggagt cagcgcagag ttcgtggacg
    11401 aagcgtctgc gaaactcaca ccgggcaaat atgcagttat tgctgacatc gatgaggagt
    11461 gggtgacccc cctggatatc gcgatggaaa agctcggtgg tcaggtcttc cgcgctgcga
    11521 gagaggatgt ggaaatcggg cagatgagag ccgacattga tgagttagac cgggagctga
    11581 cacaactgga agcggaaatg aataaggcca gtagtgaaag gaaggccaag ctccaggcga
    11641 aaattgacaa actgaaagca gaacgacaaa aaaagagaac gcgggctcag cagcggttgg
    11701 aacagattaa gaaagaacac gaagccaagg tgcaggccct gaaggagaaa gcggcaaagg
    11761 ctcgtggcga aagaaaggca gccatggatg cccgggcggc cgagatgaag aaggattttc
    11821 aacaggctgt gagcaagtgg aagatctcat aggccgaaag actggagaaa ggggctaatc
    11881 ggctgaagga gaaggctaga atctgaggag tcaaacgcag gctccaaacc agatcgagta
    11941 tcgctgcccg ggtccaacaa ccccacaggc aggatagaaa gggatccgag aaggcatgag
    12001 ggccgacccg gatcaggtgc tttttttcat cgatcgtacc tcacatgcag tttcaccctc
    12061 acatggcaaa gatcctgagt tcgaatctcc agggtccgct ccgattctcc aggtaagacg
    12121 cacatggggg atttcgatcg atgacaggtt tctgcaggtc aaattttcca ggaatgactc
    12181 attcaggaca ttttctcttt aacgtcaggg ggttgagcct caagatccgc cacggtcttc
    12241 atggtcctgg ccatagtgct tatgacgtac atgatggctg ccatcacgga tgcaatggcg
    12301 atcccaaggc cgatgatccc gatacctccg tcccacgtcg caacatgacc cacgaacaat
    12361 acgggcagaa gaacgacgac aacggcaagg agccgttctt ccagggtctc gatacgatga
    12421 gtttccagcc atttcggcag ttcaagagtg ggatcaatga agagctggta cacaccaagg
    12481 gcaatgatga tcagcacaat gccaagcagg gtcagatcaa tgatctcggt catgctgacg
    12541 gcgagatatg tcgccatctc ttcaaataca aatccccccc gggcgagatg ggatacaatc
    12601 tggaacaggc tcgtgaaact gaagatgacg acaatcaccg cggcgagaag caaggccgtt
    12661 attccgagtt ccacgataaa acgagaggtc ctatacataa ttcccaggac gggtcgctca
    12721 ttcaggcgtt cgacccgatc gataccggaa gggctggaat cgggcatatg gtatcaacaa
    12781 gtctggatta tgtagtattt agaaatagcg gaaccgtcta aggcgttctt ttcaggggtg
    12841 tatgaaagct cacaatcaaa acatcatcac cgaagtcgat ggtaggctgc gttctcaatt
    12901 caccccttat aaaactatca aaaaagcttc tgaagaagac gacccctttt ggtccccagt
    12961 tgtgatgcaa agtaatgacg caatcgccct cgcccctgct ttcaacggat acgtgggacg
    13021 ctataccata cttgcctctt aagataattg ttttcaggag ggtgtcaaag tccgcctttc
    13081 caaaatccca cattgcttga ttctttattt gtctgatggc ttcctgagat gccatttcct
    13141 gaatcctttc atcactcact tcgttgagaa gcgccttgaa aaatgcgttt gaaaaagaaa
    13201 caaagtcgag tttttcaaca actctatccc attccatgta ccgggtcatt attttcccaa
    13261 ttaaggcatt ggtgcttatg ttttgctcct gtgcatctct ttcaagcaga tgatcgatct
    13321 cttctgaaag gcgaatagtt ctcaggatgg tcttccttct cttctccttc atgtctaacc
    13381 ccttccgcgt aaaaagtaca ggtgtagatt ctgtgggcct tgcactttat aaaaacactg
    13441 gtgtcagcca atgagtttga ggataaggtt aaaagcaacc ttcaactttt cgatttcgcc
    13501 tgagatgcct gaggactgta ttttccgttg ggatcgtaat gaagaaacga gcaggggaag
    13561 gggagcggta tttccaggtc tgaagttcga actttcagaa gatcctgagt gttacgcgaa
    13621 gtccatgacc ttcggctcca gggtccggag gttcacgagg accgcccgcc ccggggtcgg
    13681 ggtgatgttc acctgcttct ggaacttcgt ctgggcctgc cagcagccgg cattcacccc
    13741 gagaactccc cggtaccgcg tgatcccgct cgtgtggaca tggcccgtgt ggaggatctc
    13801 ggggaccggg tcgatgagga gccggtcctt tggctcgggg gagagcggcg tccggcggcc
    13861 gtagaccggg gcgaggtgcc tccgctcgag catctccacc atcatctcct cggggcggga
    13921 gtaggaggcc cgggggatga ggcctatcat gtcgtcgatg gaccggccgt ggtacatgag
    13981 cacccggacc ccctcgaggg agaggagcgc cgggttctcc acgaaccggc agttctccgg
    14041 gaaccccttc ctgaactcct gggggatggc aacctggggc tcggaaggcc tcaccacgtc
    14101 atggttcccg ggcgagatga cgacggctat ccccctcggg atcccggcca tgagttcccc
    14161 gagcttcagg tattgctcgt agatgtcccg gatggccagc tcctcctcct ggttcgggta
    14221 caccccgatc ccgtccacga ggtccccgca gatgaggagg tatgccgcgc ccgaggaggg
    14281 aagccactcg atgaaccgct cccaggcatc ctcgagaaag gtcgtggacc cggcatggat
    14341 gtccgagatg aggaccgcct ttcccccccg gctgctcgtg aagggggcgt gggtgagggg
    14401 cacgtcgggg cggaccagct gctctgcaaa aaggagcctc ccgtcctggg agaggcggcc
    14461 ccgcgcgccg atgacctcgt ccgggaggag ccgctcggcg tcggcgaacc cgtcccggcc
    14521 cttgttgaag aggaccggga tctgcccggt cgggtcctcg aggtccacga gccggtggcc
    14581 gttcgccgtg gtgcgcacgt cgaggaccat cccgatgacc atgcactcct cctgccggta
    14641 ccgggtgctc cgggtgaggg cctcgatggg aaccgggttg accctcgatc gcaggatctc
    14701 ggagagccgg gtgtacctgt cccggaagta ggggaagaag tcctcgaacc ggttcttctc
    14761 ggaggagtcg cccggtttcc cggcgatgac ctcgagctcg ggctcgggaa ggaaccgctc
    14821 gccgtccttc acaggggaga ggtccggaat gtgggccggg ttgacgacca gcgtcccgct
    14881 gggaacgagg gatatcaccc tctccacgag cgcggggtcg ttcctctccc ggaggtagga
    14941 gaccacgtcc gggtgcacct ggagcttcgc ctcgaggaag cgccccacga tctccccgtc
    15001 cccgagcatc actgtactat ggcccgaagg ggttcttctc gtttcgcttc cgggggccgg
    15061 gaagggaacc gtgatggcag gctggctgtc accgtttcag gaaaaacgtg cagaaatcgt
    15121 ggattcgccc aatctgcaaa gtatatgtag caatgccgcg taccacggta cggaaactcc
    15181 tatggcaggg gagacgatcc tcgggcgggt gaagggggcg tgggagggct ttcgaaggag
    15241 cgagaatccc gtcgtggcct ttgcccggga gatcctctgg gtcgttgcgg tggtgggggg
    15301 tatcgccctc ctcctcttcc tggtctgtgg gacctggccg gccgtagtcg ccgtcgagtc
    15361 cgagagcatg ctcccgaaca tgcatgtgaa cgacctggtc ttcgtcgtgg agaaggatcg
    15421 gttcggccag ctgcagacct acgagaccgg cctctccgcc ggctaccaga agttcgggaa
    15481 cttcggggac gtcatcatct accatcccaa cggtgccgca gacatccacc ccatcatcca
    15541 ccgggcaatg gcataccata acgcctcctg gtttgggatt aacatcgggg tgaaagccac
    15601 gcatgatggg tatctaacca agggtgacaa caaccgtatc cccgaccagg aatcggccat
    15661 ccaggggttc ggccccatcc agccggtgga gccggattgg atcgtgggga aggccctctt
    15721 ctcggtcccc ctccttggct atcccactct ccactacccg gaattcgccg cggtggtcat
    15781 catcctcctc gtcatccacg agatctgggc ctcccggagt gaaaaagagg aggagaaggg
    15841 gaagaaggga aggaagaccg gaaagaaagg aaggaggtga actgtcatgg ccgggatcgc
    15901 atccctcctg aaggacgtgg caggcggcca ccggctgacc gaggatgagg ccgtcatcct
    15961 catgggagcg accggccgcg atgtctggca tgtggcggag tgcgccgacc gggtgaggga
    16021 ggaaaaggtc gggcaggcc
//
LOCUS       DATGDI010000256         4304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_376774, whole genome shotgun sequence.
ACCESSION   DATGDI010000256 DATGDI010000000
VERSION     DATGDI010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376774"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1498)
                     /locus_tag="VKO45_09660"
     CDS             complement(17..1498)
                     /locus_tag="VKO45_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HMA06175.1"
                     /translation="MEVPDTPENRGSCICPGCPSFAGGACRGEILYCSTGASACGIEP
                     LGCVCPGCSVYDEYHLKTLYFCDKQPLGETGHRVRKQRASESDAAYTSIIGIKTIADT
                     GKSITVAMGSQKRMPFTLDDLHFIPAQVHRFPLNREDTVATGVTLGPGAARPLALPTP
                     ILVSGMSYGAVSRHLKLAIAGASARLGFGFNTGEGGILDEELATAPATLVMQYSTGRF
                     GITDEKLRKAAAVEIRFGQGAYPGKGSYLPAEKITPDIARIRGLQEGEAAYSPARHPD
                     MRDIGEIRGKIAWLKSLTGGVPVGAKIGCGNVEADMADLARAGADFIALDGFGGGTGA
                     TELAVRENVGIPLLAALPRAHRLLRSLGLRERITLIGGGGLRTSADFAKCLALGADAV
                     YVGTAAMIAVNCEQYRICHTGRCPTGVTTQDPALEGQLDVPTGIDRLCNYIRVSTEEM
                     AEFARITGKNDVHALDAADLVALEEELARIVGCAWIGWAGTKE"
     gene            complement(1561..2133)
                     /locus_tag="VKO45_09665"
     CDS             complement(1561..2133)
                     /locus_tag="VKO45_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMA06176.1"
                     /translation="MARQPGPVAICLIFILFGVIWNLSMQVLLPALVRDPLYTTLFAW
                     KDWAFLAIAALLLFLLMRHSTGRLSRSEALYRTLVETSGSIILTMDREGNITFFNRRA
                     EEVFGFRREEILGKNAIGTIVPATESSGRDLDQMMQELLSSPGQFLKNRNENVTSNGK
                     RVWVTWTNRALCDETGFPMGVVSVGTQETG"
     gene            2350..2607
                     /locus_tag="VKO45_09670"
     CDS             2350..2607
                     /locus_tag="VKO45_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2769 domain-containing protein"
                     /protein_id="HMA06177.1"
                     /translation="MAKMSEPELEKTVASLKARCICGKCPTYNDCMKGKAEIFFCTLG
                     KTGCTVTKKACLCPACPVTPIMGFKHGYYCIKGSEKEIRGL"
     gene            complement(2662..3144)
                     /locus_tag="VKO45_09675"
     CDS             complement(2662..3144)
                     /locus_tag="VKO45_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HMA06178.1"
                     /translation="MRTNRHEYFLDLALRCAHQGTCLRRNFGAIIVDEFNTIVSTGYT
                     GAPRKQKDCTEIQKCWRKEHHIPSGSNYERCRSVHAEMNALIQAGKQARGCTLYLAGY
                     DVETEDLTKIWPCFLCSKMLVNSGVEKIVMRTGKSEYVEMDPEDLYRMRGAEALGEEG
                     "
     gene            3347..4075
                     /locus_tag="VKO45_09680"
     CDS             3347..4075
                     /locus_tag="VKO45_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA06179.1"
                     /translation="MPNVIEVKGLTKTYGDVTAVKNVSLSVGKGELFGLLGPNGSGKT
                     TMIKCLTGQLKPTEGAIRVMGVDAVGEPVRVRELVGITPEQETPPSFLTAEEYLHFVG
                     HIRRIPDIEAQCAFWFDFLEFGDKRDVLCKDLSRGTRQKLMVAQAFLHRPELVLIDEP
                     LINLDPLMQRKIKDYLTGYVKGGGTVFISTHILEIAQEICTRIGIIHRGNLVREGTVA
                     DLRGSSRSLEDVFLSATRGERQDA"
BASE COUNT          761 a         1355 c         1379 g          809 t
ORIGIN      
        1 gcctttcctt ttctccttat tcctttgtgc ctgcccatcc aatccaggcg catcccacga
       61 tccgggcgag ctcctcctcc agggccacga ggtcggccgc atccagggcg tggacgtcgt
      121 ttttccccgt gatccgggcg aattccgcca tctcctccgt ggagacccgg atgtagttgc
      181 agagccggtc gatgccggtc gggacgtcga gctgcccttc gagggccggg tcctgggtcg
      241 tcaccccggt cgggcagcgg ccggtgtggc agatccggta ctgctcgcag ttcacggcga
      301 tcatggccgc ggtcccgacg tacacggcgt ccgcacccag ggcgaggcac ttcgcaaagt
      361 ccgcggaggt ccgcaggccc ccgccaccga tgagggtgat ccgctcgcgc aggcccaggg
      421 agcggaggag gcggtgggcc ctggggagag ccgcgaggag ggggatgccc acgttctccc
      481 tcaccgcgag ctccgtcgcc cccgtcccgc ccccgaagcc gtccagggcg atgaagtccg
      541 cccccgccct ggcgaggtcg gccatgtctg cctcgacatt cccgcacccg atcttcgcgc
      601 ccaccgggac gccacccgtc agggacttga gccaggcgat cttcccccgg atctccccga
      661 tgtcccgcat gtccgggtgg cgggccggcg agtacgcggc ctcgccctcc tgcaaccccc
      721 ggatccgggc gatgtccggg gtgattttct ctgccggaag gtagctcccc ttgccagggt
      781 atgccccctg cccgaaccgg atctccacgg ctgccgcctt cctgagcttc tcatcggtga
      841 tcccgaacct cccggtcgag tactgcataa cgagggtggc gggcgccgtg gccagttcct
      901 cgtcgaggat gcccccctcg ccggtattga acccgaatcc gagcctcgcc gaggcaccgg
      961 cgatggcgag cttcaggtgc cgcgagacgg ccccgtagct catcccggag acgaggatgg
     1021 gggtggggag ggcgagggga cgggccgcgc cggggccaag ggttaccccc gtggcgaccg
     1081 tgtcctcacg gttgaggggg aaccggtgaa cctgggccgg gatgaagtgg agatcatcga
     1141 gggtgaaggg catccgtttc tgcgaaccca tggcgacggt gatgcttttc cccgtgtccg
     1201 cgatggtctt gataccgatg atcgatgtat aggcggcatc actctccgag gctcgctgct
     1261 ttcgcacccg gtgaccggtc tcccccaacg gctgcttgtc gcagaagtag agggttttga
     1321 ggtggtactc atcgtacacc gagcacccgg ggcagacgca gcccaggggt tctatcccgc
     1381 atgcactcgc cccggtgctg cagtagagaa tttcccccct gcaggcccct ccggcgaaac
     1441 tcgggcaccc cgggcagatg cacgagcccc ggttctcggg tgtatcggga acctccatga
     1501 tcgcatctcc ttgccggcac tccccttgaa gctatcccta tctccttgcg aaagtggttc
     1561 tcagccggtt tcctgcgtcc ccaccgagac gacacccatg ggaaacccgg tctcgtcgca
     1621 gagagcccgg ttggtccagg tcacccagac tctctttcca ttcgaggtga cgttctcgtt
     1681 ccggttcttc aggaactgcc cgggggagga gagcagttcc tgcatcatct ggtcgaggtc
     1741 ccggccggag ctctcggtgg ccgggacgat ggtcccgatg gcgttcttcc cgaggatctc
     1801 ctccctccgg aaaccgaaga cctcctcggc ccggcggttg aagaaggtga tgttcccctc
     1861 ccggtccatg gtgaggatga tgcttcccga ggtctccacg agggtccggt acagggcctc
     1921 ggaccgggag agcctgccgg tggagtgccg catgagcagg aacaggagga gggcggcgat
     1981 ggcgaggaag gcccagtcct tccaggcgaa gagggtcgtg tacagcgggt cgcggacaag
     2041 cgcgggaagc aggacctgca tggaaaggtt ccagatgacg ccgaacagga tgaagatgag
     2101 gcagatggcg accgggcccg gctggcgggc caatacggca cctcgtcgag gagaactcac
     2161 ggggatcacc tgaggagatc ttcgtatgcc ttcctataaa ggaagccggg tccggtccgc
     2221 aggggaaagg attcccgtcc cgaataccgg tgctgggtgc aaatctttat ataggccggt
     2281 gaaccggact agttcaatcc tcctgaaagg agtgttttca tggacaaatt cgaggaaatg
     2341 tcgcagaata tggcaaagat gtccgagccg gagctcgaga aaaccgtcgc atccctgaag
     2401 gccaggtgca tctgcgggaa gtgccccacc tacaatgact gcatgaaggg gaaggcggag
     2461 atctttttct gcaccctggg gaaaaccggg tgtaccgtca cgaaaaaagc ttgcctgtgc
     2521 ccggcctgcc cggtgacacc gatcatgggg ttcaagcacg gatactactg catcaaggga
     2581 tcggagaagg agatcagggg cctgtaggga aattcaccat tttccggttc gtgactcggg
     2641 gacacctggg ggcatcaggg ttcagccctc ctccccgagc gcctcggcgc ctctcatccg
     2701 gtagagatcc tccggatcca tctccacgta ctcggatttc ccggtcctca tcacgatctt
     2761 ctcgaccccg ctgttcacga gcatcttcga gcagaggaag cagggccaga tcttcgtgag
     2821 atcctcggtc tcgacgtcgt acccggcaag gtagagggtg cacccccggg cctgcttccc
     2881 cgcctggatg agggcattca tctccgcgtg cacgctcctg caccgctcgt agttggagcc
     2941 cgagggaatg tggtgttcct tcctccagca cttctggatc tcggtgcagt ccttctgctt
     3001 ccggggggcg cctgtatacc ccgtggagac gatggtgttg aactcgtcga cgatgatggc
     3061 cccgaagttg cgccgaaggc aggtgccctg gtgggcgcac cggagggcga ggtcaaggaa
     3121 atactcgtgc ctgttcgtcc tcatccatcc aagaatgggt ggggatgcgt aaagttattt
     3181 tcgccgcggg cccggccatc agggccggcc cgggagggcg gccccggagt gtctgcccga
     3241 gatttactgc ttggatgaaa accgaatcct ggctgctttt tacggctggg ttcccgcata
     3301 aatcattatt gcaggggagg gattaccggg aaatggggga ataccgctgc cgaatgttat
     3361 cgaagtgaaa ggcctcacga agacctatgg cgacgtgacg gccgtgaaaa acgtctcgct
     3421 ctcggtcggg aagggagagc ttttcgggct gctcggcccg aacgggtccg ggaagaccac
     3481 catgatcaag tgcctcaccg ggcagctgaa gccgaccgag ggtgccatac gggtcatggg
     3541 ggtcgacgcc gttggggaac cggtccgggt gcgggagctc gtggggatca cccccgagca
     3601 ggagaccccg ccctccttcc tcaccgccga agagtacctc cacttcgtgg ggcatatccg
     3661 gaggataccg gacatcgagg cgcagtgcgc cttctggttc gatttcctgg agttcgggga
     3721 caagagggac gtgctctgca aggacctctc ccgggggacg aggcagaagc tcatggtggc
     3781 ccaggccttc ctccaccggc cggaactcgt gctcatcgat gagccactca tcaacctgga
     3841 ccccctcatg cagcggaaga tcaaggacta cctcacgggg tacgtgaagg gagggggcac
     3901 ggtcttcatc tccacccaca tcctcgagat cgcccaggag atctgcacga ggatcgggat
     3961 tatccaccgg gggaacctcg tccgggaagg gacggtggcc gacctgaggg gatcctcgag
     4021 gagcctggag gacgtcttcc tctcggccac ccgcggggag aggcaggatg cctgagatca
     4081 tcgaagatcc ccttcgggga tcttctcaaa tcgcgttccc cgcgaacacg attcctcgga
     4141 aatccccaac ggggattttc tcgaatcgtg ttcccccgaa cacgatattt gccgccatgc
     4201 tcagggagga gtggcggatc cactccacca tgttcggctc ggccgggttt gccctctttc
     4261 ccctgttcct cgtctttctc gccgccgtaa ccgccctctt ctgg
//
LOCUS       DATGDI010000257         1653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_406271, whole genome shotgun sequence.
ACCESSION   DATGDI010000257 DATGDI010000000
VERSION     DATGDI010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406271"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VKO45_09685"
     CDS             <1..378
                     /locus_tag="VKO45_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06180.1"
                     /translation="GTSRETMERALRDGHDLLLDVDTRGARALKRAWPGGIFVFVIPP
                     SIEELERRLRGRGSETEETIRRRFDNALSEIREIVWYDYIIMNDKLDAAIERLRSIYV
                     AETCRRERMMVKIKPFFDRSGGN"
     gene            381..653
                     /gene="rpoZ"
                     /locus_tag="VKO45_09690"
     CDS             381..653
                     /gene="rpoZ"
                     /locus_tag="VKO45_09690"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HMA06181.1"
                     /translation="MARVTVEDALKEARNRFALVILAGQRTRQILKGSQILVENRDNK
                     PVVTALREVAAAQVRYSHPELLDRVEEQARQIGDEMGFIGDEDDSE"
     gene            634..1392
                     /gene="pyrF"
                     /locus_tag="VKO45_09695"
     CDS             634..1392
                     /gene="pyrF"
                     /locus_tag="VKO45_09695"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HMA06182.1"
                     /translation="MRTIPSKEARSRLIFALDMGDGIDETLHWVERLRDHIGVYKVGK
                     EAFTHFGPAIVRRIQDLGGRVFLDLKFHDIPNTVAKAAEGAVKLGVAMFNVHALGGRE
                     MMEKTVSAAEKTAREAGVEKPLILAVTVLTSLNDRDLLELGFHQTTQGLAAHLAAAAC
                     RAGIGGVVASPQDILSIRGSCGPDFAIVTPGIRLGGGEVEKDDQKRIDTPREAIRNGA
                     DYIVVGRPIRLAKDPVEAADRIVEEIALGLADRG"
BASE COUNT          340 a          481 c          542 g          290 t
ORIGIN      
        1 gggacctccc gggaaaccat ggagagagcc ctcagggacg gccacgatct gctgctggat
       61 gtggacacgc gcggggcccg ggcgctgaag agggcctggc cggggggcat tttcgtcttt
      121 gtcatccccc cttccatcga ggagctcgag cggcgcctcc ggggaagagg ctccgagacg
      181 gaggagacga tccgccggcg cttcgataat gcgctcagtg agattcgcga gatcgtgtgg
      241 tatgattata tcattatgaa tgacaaactg gatgcggcca ttgagcgcct gcggtccatc
      301 tatgtagcgg aaacgtgcag gcgtgaacgc atgatggtga agattaaacc tttctttgat
      361 cgatcgggag gcaactaaat atggcaagag ttaccgttga agacgcactg aaagaggcaa
      421 ggaaccgctt tgccctcgtg atcctggcag gtcagcggac ccgccagatc ctcaagggat
      481 cgcagatcct ggtggaaaat cgggacaaca agcccgtggt cacggcactg cgcgaggtgg
      541 ctgcggcaca ggtgaggtat tcccatccgg agctcctgga cagggttgag gaacaggcga
      601 gacagatcgg cgacgagatg gggttcatcg gcgatgagga cgattccgag taaggaggcc
      661 cgttcgcgcc tcatctttgc cctggacatg ggcgacggca tcgacgagac cctgcattgg
      721 gtcgagcgcc ttcgggacca catcggggtc tacaaggtcg gcaaggaggc ctttacccac
      781 ttcgggcccg cgattgtccg gcggatccag gacctggggg gacgggtctt tctcgacctc
      841 aagttccacg acattcccaa tacggtcgcc aaggccgccg agggggccgt gaagctcggt
      901 gttgccatgt tcaacgtcca cgccctcggg gggcgggaaa tgatggagaa gaccgtctcg
      961 gccgcggaaa agacggcccg cgaggccgga gtggaaaagc ccctcatcct ggccgttacg
     1021 gtactgacca gtctcaacga ccgggacctc ctcgagctcg ggtttcacca gacgacacag
     1081 gggcttgcgg cacatctcgc ggccgcggca tgccgcgccg gcataggcgg tgtggtggcc
     1141 tcaccccagg atatcctgtc gatccgcggg agctgcggcc ccgattttgc catcgtaacc
     1201 cccggcatcc gcctgggcgg tggcgaggtg gagaaagacg accagaaacg gatcgacacc
     1261 ccccgcgagg cgatccgcaa cggtgccgac tacatcgttg tgggccggcc catccggctg
     1321 gcgaaggacc ccgtcgaggc ggccgaccgc atcgtcgagg agatcgccct gggtctggcg
     1381 gacaggggat gatcctggtc tattccgtct atccggtctg tcccgtctat cccgtcccga
     1441 ttacagaaac gtgtcgcgtg tcgcgggtag cgtgtgatga aagcccgtaa tcacaatcct
     1501 cctcttaaca agccacaagc cacaaagcac tagccacatg caagtcgtct acggcatcaa
     1561 tcccgtctcg gagatcctca gaagcgaggg gggcaatgtc aggaaaatca ttctcgccga
     1621 gggacggcgc ggcgaggatg tgaagcggat cct
//
LOCUS       DATGDI010000258         2763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_28793, whole genome shotgun sequence.
ACCESSION   DATGDI010000258 DATGDI010000000
VERSION     DATGDI010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28793"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VKO45_09700"
     CDS             <1..392
                     /locus_tag="VKO45_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMA06183.1"
                     /translation="DPLSRGYYPEPTIVTGLPQGNSLARQELFVPFLVIDTFSTLGEA
                     LEKANDTEYGLTAGIFSEDEAEVETFFREIRFGVCYSNRRGGATTGAWAGRQPFGGWK
                     ASGSTGKGVGGPYYLLSYLREQGQTRL"
     gene            454..1779
                     /locus_tag="VKO45_09705"
     CDS             454..1779
                     /locus_tag="VKO45_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06184.1"
                     /translation="MTAEIVIMNREAVALAADSAVSLMAGPGDKPQKIFTSADKIFEL
                     TRDHPACIMIYNNAAFMGIPWQTLITLYRATLPATPFDTLEEYAKHFIDFLTKGEGLI
                     PESVQQRFFVDGVYRTFLFIRYLIQQNASAVLAEKGQISEEEIQVVTSTIIDDIFGKL
                     SGAPFSPTMDEAYAKELADAYGEWIVKAITEVFERIPLSPDNAQKLAQMAVFFYVKSL
                     EGFEPLERDFSGVVFAGFGKRDLFPSMQAFLVEGMIRKRLKHLEVEKNQVGFENGAII
                     IPFAQREMVDIFMSGLDPRFTGTLLAGLAKTFQEYPQLIIESIEKLTDEEKQALKGKF
                     GETSNAIIQEIQNQLASYQAFNANPIVNVVAALPRSDLASMAESLVNLTSLKRRVSMQ
                     AETVGGPVDVAVISRSDGFIWIKRKEYFRRELNPGYLAGRSGRDHAGKG"
     gene            1763..1963
                     /locus_tag="VKO45_09710"
     CDS             1763..1963
                     /locus_tag="VKO45_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06185.1"
                     /translation="MPGKDKKKGIICHSPVDFERIFLPRSFERKEKEKILKEPGGFGK
                     NLEKELIASIRKGRGPRTKGKK"
     gene            complement(1987..>2763)
                     /locus_tag="VKO45_09715"
     CDS             complement(1987..>2763)
                     /locus_tag="VKO45_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HMA06186.1"
                     /translation="FDYADVWFRDFGPSFVVNRKDRTTAMVAWEFNAWGGKYPWIVRD
                     TRVPSCLNAQLGLPLYRPGIVLEGGSVDVNGRGTVLTTEQCLLNPNRNPGLARDAIES
                     YLEDYLGTPQVIWLEGGIAGDDTDGHVDDVARFVNPSTVVFAVETDPEDENFRVLREN
                     ERILREARDQDGDPLAVIPLPMPGPLGGDARLPASYLNFFIGNRVVLVPVFSDPNDGK
                     ALQVLGGLFPGRKIIPIDCRALVEGKGAIHCITREQPRVG"
BASE COUNT          540 a          878 c          886 g          459 t
ORIGIN      
        1 aagacccgct ctcgagaggc tattacccgg aacccaccat cgtgaccggg ctcccgcagg
       61 ggaattccct ggcgaggcag gagctcttcg ttcccttcct cgtcatcgat accttctcca
      121 ccctcggaga ggctcttgag aaggcgaacg acaccgagta cgggctcacc gccggtatct
      181 tctccgagga cgaggcggag gtggagacct tcttccggga gatccggttc ggggtctgct
      241 actccaaccg gcggggtggg gcgacgaccg gcgcctgggc gggccgccag cccttcgggg
      301 ggtggaaggc gagtggctcg acgggaaagg gggtcggggg accgtactac ctgctctcgt
      361 acctgcggga gcaggggcag acgaggctat gagcttggga aaccttcatc agcccgtccg
      421 gcatcggatg tatccggaga aggtgatcat tccgtgaccg ccgaaatcgt catcatgaac
      481 cgggaggcgg tggccctcgc cgccgacagc gccgtctccc tcatggcagg ccccggggac
      541 aaaccccaga agatcttcac ctccgcggac aagatcttcg agctcacccg ggaccacccg
      601 gcctgcatca tgatctacaa caacgccgcc ttcatgggca tcccctggca gaccctcatc
      661 accctctacc gggccaccct gccggcgacc cccttcgata cccttgagga gtacgcgaaa
      721 cacttcatcg acttcctgac gaagggggag gggctcatcc ccgagtccgt gcagcagcgc
      781 ttcttcgtgg acggcgtgta ccggaccttc ctcttcatcc ggtacctcat ccagcagaac
      841 gcctcggcgg tccttgccga gaaggggcag atcagcgagg aggagatcca ggtggtcacc
      901 tcgaccatca tcgacgatat cttcgggaag ctctccgggg cccccttcag ccccaccatg
      961 gacgaggcct atgcaaagga gcttgccgat gcctacggcg agtggatcgt gaaggccatc
     1021 accgaggtct tcgagcggat ccccctctcc ccggataatg cccagaagct tgcgcagatg
     1081 gcggtgttct tctacgtcaa atccctggag gggttcgagc ccctggagag ggacttctca
     1141 ggggtggtct tcgccgggtt cgggaagagg gacctcttcc cctcgatgca ggccttcctc
     1201 gtcgaaggga tgatccggaa gcggctcaag cacctggagg tcgagaaaaa ccaggtgggc
     1261 tttgagaacg gggcgatcat catccccttc gcccagcggg agatggtcga catcttcatg
     1321 tccggcctgg acccccggtt caccgggaca ctccttgcgg gcctcgcgaa gaccttccag
     1381 gagtatcccc agctgatcat cgaaagcatc gagaagctta ccgacgagga aaagcaggcc
     1441 ctcaagggga agttcggcga gacctcgaac gcgatcatcc aggagatcca gaaccagctc
     1501 gcctcctacc aggccttcaa cgcgaacccc atcgtaaacg tggtcgcggc cctcccccgg
     1561 tctgacctcg cgtcgatggc agaatccctc gtcaacctca cctccctgaa gcggagggtg
     1621 tccatgcagg cagagaccgt cgggggaccg gtggatgtcg cggtgatctc gaggagcgac
     1681 ggcttcatct ggatcaagcg gaaggagtat ttccggcggg agctgaaccc cggctacctg
     1741 gccgggagaa gcgggaggga tcatgccggg aaaggataaa aagaagggga tcatctgcca
     1801 ttcgccggtg gacttcgagc gcatcttcct gccacgctcc ttcgagagga aggagaagga
     1861 gaagatcctg aaggagccag ggggtttcgg gaagaacctg gagaaggagc tcatcgcctc
     1921 aatcaggaag ggcaggggcc cgagaacgaa gggaaagaag tagggcgaac cggcacggga
     1981 aggaggtcat cccacccggg gctgctcacg ggtgatgcag tggatcgccc ccttgccctc
     2041 aacgagggca cggcagtcga tgggaatgat ctttctcccc gggaagaggc cccccaggac
     2101 ctggagggcc ttcccgtcgt tcggatcgga gaagaccggg acgaggacca cccggttccc
     2161 gatgaagaag ttcaggtagc tcgcggggag cctggcgtcc cccccgagag ggccgggcat
     2221 ggggaggggg atgaccgcca ggggatcccc gtcctggtcc cgcgcctccc tgaggatgcg
     2281 ctcgttctcc cggaggaccc ggaagttctc atcctccgga tccgtttcga cggcgaatac
     2341 cacggtggac gggttcacga accgggccac atcgtcgaca tggccatccg tgtcatcgcc
     2401 ggcgatcccc ccctccagcc agatgacctg cggggtaccc aggtaatcct ccaggtatga
     2461 ttcgatggcg tcgcgggcga ggcccgggtt ccggttgggg ttcagcaggc actgctccgt
     2521 ggtgaggacg gtccccctcc cgttcacgtc gacggacccg ccctcgagga cgatccccgg
     2581 ccggtacagg gggaggccga gctgggcgtt caggcacgac gggacccggg tgtccctgac
     2641 aatccacggg tatttcccgc cccaggcgtt gaactcccag gccaccatag ccgtcgtccg
     2701 gtccttccgg ttcacgacga aggaggggcc gaagtcccgg aaccagacat ccgcatagtc
     2761 gaa
//
LOCUS       DATGDI010000259         4307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_115939, whole genome shotgun sequence.
ACCESSION   DATGDI010000259 DATGDI010000000
VERSION     DATGDI010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115939"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VKO45_09720"
     CDS             <1..608
                     /locus_tag="VKO45_09720"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: protein motif:HMM:NF004060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HMA06187.1"
                     /translation="TMLIGLGLGPGDPELLTLRAVRLLREADAVFVPGGIAQKLVAPY
                     REAEVLAFPMTGDEAAIRRAIGESAEKIARVAAKGVAVFALLGDPNVYSTYARLLGVI
                     RERHPEIPCKTVPGVSSITAFASVAGITLSGGFSVSDGKGERGRILLKVRRPREEAER
                     LAREGFREFVLVERMFLDGERVYRGELPEESTYFSILYAGR"
     gene            610..1326
                     /locus_tag="VKO45_09725"
     CDS             610..1326
                     /locus_tag="VKO45_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4/precorrin-4
                     C(11)-methyltransferase"
                     /protein_id="HMA06188.1"
                     /translation="MEPVYFVGAGPGDPDLITVKGLGLLESADLLLYTGSLVNPELVG
                     RSQAAEKLDSHGMSHADIIARLEKAAREGKKVVRLHSGDPALYGAIVEQILALEDRGI
                     PSVVVPGVSSLFAAAAALGTQLTLTGVSESLIVTRPAGETLEEDRIPALSSLGETLAI
                     FLGGDRIADVVERLRCPPETPAAVIYHASWGDQKVVRGTVADIAKRAEEAGIRRTALI
                     IVGKVVEPRTSGFRRSHLYP"
     gene            1323..2195
                     /gene="cbiG"
                     /locus_tag="VKO45_09730"
     CDS             1323..2195
                     /gene="cbiG"
                     /locus_tag="VKO45_09730"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HMA06189.1"
                     /translation="MTPTCVIALPRFLKRAGKIGKHLGADLIPYGPGVFLSAFQSYTG
                     IVAVMSAGIVVRSIAPLLGNKWTDPAVVVVSPDLAWAIPIAGGHHGANRIARSLGELG
                     IRPVITTATETAGKMSVEGIADERGLRVLNPPSTRAVNAAILDGDVPVFPVPGPGMVI
                     AGPAVSVLLRNGEYTVGIGCRRGLPEDAIVAGIRAGLSEAGISPGDVFAYGTTVRKIR
                     ETGLVRAVESLGGNLVFLDDAAIGAQPAPTPSRAGLLGLKGVAEPCALALSMRGVLVA
                     AKKNYGGVTLAIAR"
     gene            2179..2985
                     /gene="cobJ"
                     /locus_tag="VKO45_09735"
     CDS             2179..2985
                     /gene="cobJ"
                     /locus_tag="VKO45_09735"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HMA06190.1"
                     /translation="MPSHADRPRGPAPPGGGRLAIVGIGPGGPDQMTARARDVIAGAD
                     CVVGNGVYLDQVAPLLQGKEILRSSMGKEVERAQRAVELACSRSVAVVSGGDPGVYGM
                     ASIVLEVLERSGLDVPVEVVPGVTAATAAASRLGAPLSGDFVVMSLSDLLTPRAEIER
                     RLDLAFRMGVPVVLYNPRSRGRKDHLSAALALAARHLPQETPVGFVKDAYRKGEQMAV
                     TTLGEAREGIGEVDMHATVIIGGRESRIWRRGKDAKGIITPRGYERKYLY"
     gene            2963..3604
                     /locus_tag="VKO45_09740"
     CDS             2963..3604
                     /locus_tag="VKO45_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HMA06191.1"
                     /translation="MRESTFIDPGARTGAAREISRKSRAIARAAIGDRTPEDRIRQRC
                     AIAVGDLAMAGLLRFRKDPVGAGLEALRREAPIITDIRMAQAGILKTGHRSSVLCALD
                     AGASAREGMTRTAAGFHALADRVGKSIVVVGNAPSALFAVCELVDRGREPALIIGTPV
                     GFVQAAESKELLRTLPVPSISTEGTRGGSPVAVAAMNEIITVWMQESGQWKTP"
     gene            3589..>4307
                     /locus_tag="VKO45_09745"
     CDS             3589..>4307
                     /locus_tag="VKO45_09745"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HMA06192.1"
                     /translation="MEDPVTGFRYPDAWVRACRAPALLPLVEGGLALLTADGTVLRRG
                     FTTGTTAAAACKAAILSLGGDGVEEVSVTLPCGITVDLPARGREGEGSAVKDAGDHPS
                     DVTGGLEFIASARPSEEELVLEFGEGIGRYARDVPGHAIGSPAVSGPALSSITGASLE
                     GLEGAGCHGAVVSLRVPRGREVASRTLNPRLGIEGGISLLGTTGLVEPWDEHLVSSVL
                     ERVRAAETPVLTTGRTGLMYSR"
BASE COUNT          646 a         1448 c         1552 g          661 t
ORIGIN      
        1 gaaccatgct cataggcctt ggccttggcc ccggcgatcc cgagctcctc accctgcggg
       61 cggtccggct ccttcgcgag gcagatgccg tcttcgttcc cgggggaatc gcgcaaaaac
      121 tcgtggcacc ctaccgggaa gcagaggtcc ttgcctttcc catgaccggc gacgaggcgg
      181 cgatccgcag ggccatcggg gagtcggcgg agaagatcgc ccgggtggcg gcgaaggggg
      241 tcgcggtctt tgccctcctc ggcgatccaa acgtctattc cacctacgcc cggctcctcg
      301 gggtgatccg ggagaggcac ccggagatcc cgtgtaaaac ggtccccggc gtctcctcca
      361 tcaccgcctt tgcctcggtc gcggggatca ccctctccgg tggcttctcc gtttcggacg
      421 ggaaggggga gagagggcgg atcctcctca aggtgcgccg gccgagggag gaggcggaac
      481 ggctcgcccg ggagggcttc cgggagttcg tgctcgtgga acggatgttc ctcgacggtg
      541 agcgggtgta ccgcggggag ctccccgagg agagcacgta cttctccatc ctctatgcgg
      601 ggcgctaaga tggagccggt ctatttcgtc ggtgccggcc cgggggaccc cgatctcatc
      661 acggtgaagg ggctcgggct cctcgagagc gccgacctcc tcctgtacac cggctccctc
      721 gtgaatccgg aactcgtggg gaggtcgcag gccgccgaga agctggacag ccacgggatg
      781 agccacgcgg acatcatcgc ccggctcgag aaggcggccc gggaggggaa aaaggtggtc
      841 cggctccaca gcggggatcc cgccctctac ggggccatcg tcgagcagat cctggccctt
      901 gaggaccggg ggatcccatc ggtggtggtg ccgggggtct cctccctctt cgccgccgca
      961 gcggccctcg ggacccagct caccctcacg ggggtctcgg agtccctcat cgtcacccgg
     1021 ccggccgggg agaccctgga ggaggaccgg atcccggccc tctcctccct cggcgagacc
     1081 ctcgccatct tcctgggagg ggatcggatc gcagacgtcg tggagaggct gcgctgcccg
     1141 ccggagacgc ccgccgccgt catctaccac gcgtcatggg gggatcagaa ggtggtgcgg
     1201 gggacggtgg ccgacatcgc gaaacgggcg gaggaggccg gtatccgccg caccgccctc
     1261 atcatcgtcg ggaaggtggt ggagccccgc acctcgggtt tcaggaggtc gcacctctac
     1321 ccatgacccc cacctgcgtc atcgcccttc cccggttttt gaagagggca gggaagatag
     1381 ggaaacacct cggggcagac ctgatcccct atggaccagg tgtctttctg tcggcctttc
     1441 aatcgtacac cgggatcgtg gcggtcatgt cggccgggat cgtggtccgc tccatcgctc
     1501 ccctgctcgg gaacaagtgg accgaccccg cggtcgtggt ggtcagcccc gatctggcct
     1561 gggcaatccc catcgccggc gggcaccacg gggcgaaccg gatcgcgagg tccctcgggg
     1621 agctgggaat ccggccggtg atcaccaccg cgaccgagac cgccgggaag atgtccgtcg
     1681 agggcatcgc cgatgagcgg ggactccggg tcctcaaccc cccttccacg agggctgtga
     1741 acgccgcgat cctggacggg gacgtccctg tattccctgt accggggccc ggcatggtca
     1801 ttgccggccc ggcggtctcg gtcctcctga ggaatgggga gtacaccgtc gggatcgggt
     1861 gccggagggg actaccggag gatgccatcg tcgcgggtat ccgtgcgggg ctttccgaag
     1921 cggggatctc cccgggcgat gtcttcgcct acgggaccac ggtccggaag atccgggaga
     1981 cgggccttgt ccgggccgtg gagtccctgg ggggaaacct cgtattcctc gatgatgccg
     2041 ccatcggtgc ccagcctgcg ccgacgccct cccgggcagg gctcctcggg ctcaagggag
     2101 tcgccgagcc atgcgctctt gccctctcca tgagaggggt tcttgttgcc gcaaagaaga
     2161 actatggagg ggtaaccctt gccatcgcac gctgaccgcc cccgcggccc cgcccctccg
     2221 ggcgggggga ggcttgccat cgtcgggatc gggcccggcg gcccggacca gatgaccgcg
     2281 agggcccggg acgtcatcgc cggagccgac tgcgtcgtgg ggaacggcgt gtacctggac
     2341 caggtcgccc ccctccttca ggggaaggag atcctccggt cctcgatggg gaaggaggtg
     2401 gagcgggcac agagggccgt cgagctcgcc tgctcgagga gcgtggccgt ggtgagcggg
     2461 ggggaccccg gggtctatgg gatggcctcc atcgtgcttg aggtcctgga gcggtccggc
     2521 ctcgacgtcc ccgtggaggt ggtgcccggg gtgaccgccg ccacggcggc agcctcccgg
     2581 ctcggggcac ccctctccgg tgatttcgtg gtcatgagcc tctcggacct cctgaccccc
     2641 cgggcggaga tcgagcgcag gctcgacctc gccttcagga tgggagtccc ggtggtactc
     2701 tacaacccga gaagccgggg ccggaaagat cacctgtcgg ctgccctcgc cctcgcggcc
     2761 cgccacctgc cgcaggagac tccggtcggg ttcgtgaagg atgcataccg aaagggtgaa
     2821 cagatggcgg tcaccaccct cggagaggcg agggagggaa tcggggaggt ggacatgcat
     2881 gcaacggtca tcatcggagg ccgggagagc aggatctgga ggaggggaaa ggatgcgaag
     2941 gggatcatca cgcccagggg atatgagaga aagtaccttt attgaccccg gcgcacggac
     3001 cggggccgcg agggagatct cgcggaaaag ccgggccatc gcccgggccg ccatcgggga
     3061 ccggactccc gaggaccgga tccggcagcg atgcgccatc gccgtcggag acctggccat
     3121 ggcaggcctc ctccggttca ggaaagaccc tgtgggggcg ggcctcgagg cactccggag
     3181 ggaggccccc atcatcaccg acatccggat ggcccaggcc ggaatcctga agacagggca
     3241 ccggagctcc gtcctctgcg cgctggacgc cggagcctcg gcccgggagg ggatgacccg
     3301 gaccgcggcc gggttccacg ccctcgcgga ccgggtcggg aagtccatcg tcgtggtggg
     3361 gaatgcccct tcagccctct tcgccgtctg cgagctcgtc gacagggggc gggaaccggc
     3421 cctcatcatc ggcacgcctg tcgggttcgt gcaggccgcc gagtcgaagg agctcctccg
     3481 gacccttccc gttccctcca tctccaccga gggcacgcgg ggcgggagcc cggttgccgt
     3541 ggccgcgatg aacgagatca tcaccgtctg gatgcaagag agcggacaat ggaagacccc
     3601 gtgaccgggt tccggtaccc cgatgcctgg gtacgggcct gcagggcccc ggccctcctc
     3661 cccctggttg aaggaggcct cgccctcctc acggccgatg gaaccgtact ccggcgcggc
     3721 ttcaccaccg gcaccaccgc cgcggcggcc tgcaaggcgg ccatcctctc cctcggcggt
     3781 gacggggtgg aggaggtctc cgtcaccctc ccctgcggga tcaccgtcga cctccccgcc
     3841 cggggccggg agggggaggg ctccgcggtg aaggacgccg gggaccaccc ctccgacgtg
     3901 acgggcggtc tcgagtttat cgcgtccgcg aggccttcgg aggaggaact cgttctcgag
     3961 ttcggggagg ggatcggcag gtacgcgagg gatgttcccg ggcacgccat cgggtcaccg
     4021 gcggtgagcg ggcccgccct ctcctccatc accggggcgt ccctcgaggg gctggagggg
     4081 gccggctgcc atggcgcggt ggtctccctc cgggtccccc gggggcggga ggtggcgtcc
     4141 cggaccctta acccgaggct cgggatcgag gggggcatct ccctcctcgg gaccaccggg
     4201 ctcgtggagc cctgggacga gcacctggtc tcctcggtgc tggagcgggt gcgggccgcc
     4261 gaaacgccgg tcctcaccac gggaaggacg ggcctcatgt actcccg
//
LOCUS       DATGDI010000260         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_348249, whole genome shotgun sequence.
ACCESSION   DATGDI010000260 DATGDI010000000
VERSION     DATGDI010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348249"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VKO45_09750"
     CDS             complement(<1..449)
                     /locus_tag="VKO45_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HMA06193.1"
                     /translation="METSKGVSPRERQQGEALMENLHGGIPELLAPAGSMEALKAAVS
                     CGADAVYLGGKRFSARQYAANFDDRELGEAVEFAHGRGVRIYVTVNTLLHDREIPDAA
                     EYLISLRETGVDAVLVQDPGLATLAGEVIPDLPLHASTQMTLHSREGL"
     gene            561..1520
                     /locus_tag="VKO45_09755"
     CDS             561..1520
                     /locus_tag="VKO45_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMA06194.1"
                     /translation="MRLIFELSGEHPTLPVAELECVGRVMEARPQVAIAECPDPGAAG
                     RLSLTHAVLGFLGSCGPSVADLSALLRDLSLSPDRPFAARVKRVAGSQVREPAPAIEK
                     LVGTLIRGQVSLDQPEIEYRAILSGDRIYLGRVLLRIDRGGYDLRRPSTRPFFHPGVM
                     LPRTARAVVNLSLVLPGETLFDPFCGTGGLLGEAELIGASPLGSDADFVMVRGCRANF
                     PAIPLLLADACSLPLRSGSVDSVATDLPYGQSSWIRGGNLDDLSTRALGEIHRILRPG
                     RRAVVVTNREIREVAGTLFEVLQVHRQRVHRSLTRRITVLVKA"
     gene            1618..>3224
                     /gene="pheT"
                     /locus_tag="VKO45_09760"
     CDS             1618..>3224
                     /gene="pheT"
                     /locus_tag="VKO45_09760"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HMA06195.1"
                     /translation="MPIIKLNYQYLEQLVGKDRGTILPRLPMIGCELERLEEDHADAE
                     FFPDRPDLFSTEGVAHALRGFLSVEEGLREYPVTPSGMAFSVDPGLASIRPYLGSAVI
                     RGLDLGEAAIESLMGLQEALHWAVGRGRGKVAIGVHDLDTVKPPFRYLAAERSRSFVP
                     LDYDRPMTMEEILTEHPKGKAFAHLVRDLPRFPLIEDSRGEVLSFPPIINGELTRVTG
                     RTKNILLDTTGTDRKAVTTAVRILCTAMAETGARIESVSIDGVPCPDLSPAERVVSAE
                     ACSRLIGIPLTPSSMAGLLRKMRFGAAPASKNRVKVRVPCYRADIMHDWDIYEDVAIA
                     YGYDRFTPALPRVFTIGAKHPVTVLADTVRAIFTGLGYLEVIPFTLTNERVLFTRMQR
                     TPNPSALRVKYPISEEHTVIRTDILPLLLETLQANRHRELPQRIFAIGDVVEGRETLQ
                     KVAAVSLHPAADFSEAYAVADALLRELALSCAVGESGDPAFIDGRRGDLSVDGERRGS
                     FGEIHPSVLTAFELEHPVAALELDLRAV"
BASE COUNT          485 a         1155 c         1034 g          550 t
ORIGIN      
        1 aggccctcgc ggctgtgcag ggtcatctgg gtggaggcat ggaggggcag gtccgggatc
       61 acttcgccgg cgagggtggc gaggcccgga tcctggacga ggacggcatc cactcccgtt
      121 tcccgaaggg agatcaggta ctccgctgcg tccggtatct cccggtcgtg gaggagagtg
      181 ttcactgtca cgtagatcct gactcccctc ccgtgggcga actccacggc ctccccgagc
      241 tcccggtcat cgaagttcgc cgcgtactgg cgggccgaga acctctttcc cccgaggtac
      301 accgcgtccg ctccgcagga gaccgccgcc ttcagggcct ccatggatcc ggccggggcg
      361 agaagctccg ggataccgcc gtgaagattc tccatcaggg cttcgccctg ctgtctctct
      421 ctcgggctga cgcccttcga ggtctccatc ggggattccc atagaggtac cctcctcccg
      481 cgctcatctc cctatcggtg gggcaccctc cgggaatccg gtggtttatc tccctccgtt
      541 ccccatggta gaagagaagc atgaggctca tcttcgagct ctcgggagaa cacccgaccc
      601 tccccgttgc cgagctggag tgcgtgggga gggtgatgga ggcccggccc caggtggcga
      661 tcgccgagtg cccggatccc ggtgcggccg ggcgcctgtc cctcacccac gcggtccttg
      721 ggttcctggg ctcctgcggc ccctcagtgg cagacctctc cgccctcctc cgggatcttt
      781 ccctctctcc ggaccggccc tttgcggcga gggtgaagag ggtcgccggt tcgcaggtca
      841 gggaaccggc ccccgccatc gagaaactcg tggggaccct catccggggg caggtctccc
      901 tcgatcagcc ggagatcgag tatcgggcca ttctctccgg ggaccggatc tacctcggcc
      961 gggtgctcct ccggatcgac cggggaggct atgacctgag gaggccctcg acccggcctt
     1021 tcttccatcc cggggtgatg ctcccccgga ccgcccgtgc cgtcgtgaac ctgtccctcg
     1081 ttctgcccgg cgaaaccctc ttcgatccct tctgcgggac cgggggactc ctcggagagg
     1141 cggaactcat cggggcatcc ccgctgggga gcgatgcgga cttcgtgatg gtccgggggt
     1201 gccgggcgaa cttcccggcc atccccctcc tgctcgccga tgcctgctcc cttccccttc
     1261 gatccggttc ggtggactcg gtggcgaccg acctcccgta cggccagtcg tcctggatcc
     1321 ggggcgggaa cctcgacgac ctctccaccc gggccctcgg ggagatccac cggatcctgc
     1381 gacccgggcg aagggcggtg gtggtgacca accgggagat ccgagaggtc gccggtaccc
     1441 tcttcgaagt gctccaggtc cacaggcagc gggtacaccg gtccctcacg aggcggatca
     1501 cggtcctcgt gaaggcatga tccgggagag ggagagggac ggaatgtccc cgatgggaaa
     1561 tccgctggct atatcaggga aacgggggaa cctttaaccc gggaagggtg aggcccggtg
     1621 cccatcatca agctcaacta ccagtacctg gagcagctcg tcgggaagga ccgcgggacc
     1681 atcctcccga ggctccccat gatcgggtgc gaactcgagc gcctcgagga ggaccatgcc
     1741 gatgcggagt tcttccccga caggccggac ctcttctcca ccgagggagt cgcccatgcc
     1801 ctccggggat tcctctccgt cgaggagggt ctccgggagt acccggtcac cccctccggc
     1861 atggccttct cggtcgaccc cggcctcgcc tccatccgcc cctacctcgg gagcgcggtg
     1921 atccggggcc tcgacctcgg ggaggccgcc atcgagagcc tcatgggact ccaggaggcg
     1981 ctccactggg ccgtgggccg gggccggggg aaggtggcca tcggggtgca tgacctcgat
     2041 accgtgaagc ccccgttccg gtacctcgcc gccgagcggt cccgctcctt cgtgcccctc
     2101 gactatgacc ggcccatgac catggaggag atcctcaccg agcacccgaa ggggaaggcc
     2161 tttgcccacc tggtccggga cctgccccgg ttccccctca tcgaggactc ccggggagag
     2221 gtcctgtcct tcccgccgat catcaacggg gagctcaccc gggtgaccgg gaggacgaaa
     2281 aacatcctcc ttgatacgac cgggaccgac aggaaggcgg tgacgaccgc ggtccggatc
     2341 ctctgcacgg ccatggcaga gaccggggcc cggatcgaga gcgtctccat cgatggggtc
     2401 ccctgcccgg acctctcccc cgccgagcgg gtcgtctccg ccgaggcctg ctcccggctc
     2461 atcggcatcc ccctcacccc ctcctccatg gcaggtctcc tcaggaagat gcgcttcggc
     2521 gcggcccctg cgagcaaaaa ccgggtgaag gtccgggtcc cctgctaccg ggccgatatc
     2581 atgcacgact gggatatcta cgaggatgtc gccatcgcgt acgggtacga ccggttcacc
     2641 ccggccctgc cccgggtctt caccatcggg gcgaagcacc cggtcaccgt gcttgccgac
     2701 acggtccgtg ccattttcac cggcctcggc tacctggagg tgatcccctt caccctcacg
     2761 aacgagcggg ttctcttcac gaggatgcag cggaccccga acccctcggc cctccgggtg
     2821 aagtacccca tcagcgagga gcacacggtc atccgcaccg acatcctccc gctcctcctc
     2881 gagaccctcc aggcgaaccg gcaccgggaa ctcccgcagc ggatctttgc catcggggat
     2941 gtcgtggagg ggagggagac cctccagaag gtggccgcgg tctccctcca cccggcggcc
     3001 gacttttccg aggcctacgc ggtggccgat gccctactcc gggagctcgc gctctcctgt
     3061 gccgtgggag aatcggggga ccccgctttc attgacggtc ggaggggcga cctgtcggtc
     3121 gacggggaga ggagaggctc cttcggcgag atccacccct cggtcctcac cgccttcgag
     3181 ctggagcacc ccgtcgcggc cttggagctc gacctcagag ccgt
//
LOCUS       DATGDI010000261         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_144890, whole genome shotgun sequence.
ACCESSION   DATGDI010000261 DATGDI010000000
VERSION     DATGDI010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144890"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="VKO45_09765"
     CDS             <1..162
                     /locus_tag="VKO45_09765"
                     /inference="COORDINATES: protein motif:HMM:NF036704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06196.1"
                     /translation="FREESTNGEFIVPYSTQGNSYGVKATGPYRIAGSTATYEVSEAD
                     VLAGRTVGP"
     gene            256..1416
                     /locus_tag="VKO45_09770"
     CDS             256..1416
                     /locus_tag="VKO45_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMA06197.1"
                     /translation="MVANAPRTDEPLEVSVILPSLNEEKTIGTCISKIQEVFRDTGIR
                     GEIIISDSSTDSTPELARASGARVIHPDHAGYGNAYLAAFREARGEILVMGDADDTYD
                     FSDIPRLLAPLRAGADLVLGSRFKGVITPGSMAPLHRYIGNPLLTWLLNILYHTGFSD
                     GQTGFRAITREALSRLKLSTGGMEFASEMLIRASEQKLSIVEIPITYSPRQSPSKIHS
                     FADGWRHLRFVLLMRPIPFLATPGLLFAAIGLVLMAIFYLTGNIETSRFNSFILGTLL
                     LLGGIQAFFTGVEISVYSIVHGFLEKQGLVARIMNYHRLEEMLLAGGTLLFIGFVLGL
                     GIIARWVARGFGALGEVSNAVISMILVVVGMEVIFFAIFVSMMLLNERNGAM"
     gene            1423..>2496
                     /locus_tag="VKO45_09775"
     CDS             1423..>2496
                     /locus_tag="VKO45_09775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMA06198.1"
                     /translation="MRIAFLYDCVYPYTIGGVERRVLELGRRLAARGHEVHHFGMQFW
                     DGPPVIEREGIRIHGVCPASPLYAGGRRAVLPAARYAASLLGPLLAGGFDLIDAQQFP
                     YLHCLPAAMASRVRDIPLAVTWHEVWGPYWRDYMGWFAGRLGEMAEWAVARLPGSLVA
                     VSRTTGDRLRDLGVSRPVEILPNGLDLASIAAASPAPERWDLLFAGRLIPEKRVDLLI
                     DSLPDIVAEYPSLQVLILGEGPSRPALEGLASRRGIRDHVTFRSFVPDPTAVIGFMKS
                     GGVFVSPSVREGFGMAALEAMACGMPVVTVDHPRNAIKERVTSSTGRVARPEPGDLGE
                     KIRECLEDPSRFREGCRSFAAEYD"
BASE COUNT          412 a          818 c          764 g          502 t
ORIGIN      
        1 ttccgggaag agagcacgaa cggggagttc attgttccgt actccacgca ggggaattcc
       61 tacggggtga aggcaaccgg gccctaccga atcgcaggct cgacggcgac ttacgaggtg
      121 agcgaggcgg atgttctcgc cgggcgcacc gtggggccct gatccgggga agatgagccg
      181 ccatccgcac cgggtgagcc tatgggaaca attctgatag gtttatcctc gcccgggaat
      241 catcccttcg ataccatggt ggcgaacgct ccccgaacgg acgaacccct cgaggtctcg
      301 gtgatcctcc cctctctcaa cgaggagaag accatcggca cctgcatctc caagatccag
      361 gaagtcttcc gggatacagg catccggggc gagatcatca tctcggattc atcgacggat
      421 tccacgccgg agctcgcccg ggcctcggga gcacgggtca tccaccccga tcatgccggg
      481 tacgggaacg catacctcgc cgcattccgg gaagcacgcg gggagatcct tgtcatgggg
      541 gatgcggatg atacctacga cttctccgat atcccccggc tcctcgcccc cctcagagcg
      601 ggtgccgacc tggtattggg gtcccgcttc aagggagtga taacgcccgg ttccatggcg
      661 cccctccacc ggtacatcgg gaacccttta ctcacctggc tcctcaatat cctctatcac
      721 accgggttct ctgatggaca gaccgggttt cgcgccatca cccgggaagc cctttcccgc
      781 ctgaaactct ccaccggtgg catggagttt gcatcagaga tgctcatacg ggcatctgaa
      841 cagaagctct ccatcgtcga gatacccatc acctactccc cccggcagag tccctccaag
      901 atccacagtt tcgccgacgg gtggcgccac ctgaggtttg tcctcctcat gcgccccatc
      961 cccttcctcg cgacccccgg gcttctcttc gcggccatcg ggctcgtcct catggccatc
     1021 ttctacctca ccgggaacat cgagacctcc cggttcaact ccttcatcct cgggaccctc
     1081 ctccttctcg gggggatcca ggccttcttc accggggtgg agatttcggt ctactccatc
     1141 gtccacggat ttctcgagaa gcaggggctc gtcgcccgga tcatgaacta ccaccggctc
     1201 gaggaaatgc ttctcgccgg gggaaccctc ctgttcatcg gctttgtcct gggcctcggg
     1261 atcatcgccc ggtgggttgc ccgggggttc ggtgccctcg gggaggtctc gaacgccgtc
     1321 atctccatga tcctcgtcgt cgtagggatg gaggtcatct tctttgccat tttcgtctcc
     1381 atgatgcttc tcaatgaaag gaacggggcg atgtagggac ctatgcggat agccttcctc
     1441 tacgactgcg tctatccgta caccatcggc ggggtggaac gacgcgttct cgagctgggg
     1501 cgccgcctgg ctgcccgggg gcatgaggtg caccacttcg ggatgcaatt ctgggatggc
     1561 ccgccggtca tcgagaggga ggggatccgg atccacgggg tctgcccggc ctcccccctc
     1621 tacgcgggag gaaggcgggc cgtcctcccg gccgcccggt acgcggcctc cctcctcggc
     1681 cccctccttg cgggtggttt cgaccttatc gacgcccagc agttccccta cctccactgt
     1741 ctccctgcag cgatggcatc gagggtgagg gatatccccc tcgccgtcac ctggcacgag
     1801 gtgtggggac cgtactggag ggactacatg ggatggtttg ccgggaggct gggggaaatg
     1861 gcggaatggg ccgtggcacg cctgcccggc tcgttggtcg cggtttcccg cacgaccggg
     1921 gaccggttac gggatctcgg ggtctcccgg ccggtggaga ttctcccgaa cggcctcgat
     1981 ctcgcctcca ttgccgcggc gtccccggct cccgagcgct gggacctcct ctttgccggg
     2041 aggctgatac ccgaaaagcg ggtagatctc ctcatcgact cactcccgga tatcgttgct
     2101 gaatatccct ctctccaggt actgatcctg ggcgaaggcc cctcgcgtcc cgccctggag
     2161 ggtctcgcat cccgccgggg tatccgggat cacgtcacct tcaggagctt tgttccagat
     2221 cccacagcgg ttatcgggtt catgaagagc ggcggtgtct ttgtttcgcc atcggtcagg
     2281 gagggattcg ggatggctgc cctggaggcg atggcatgcg gcatgccggt ggtgaccgtg
     2341 gaccacccgc gaaacgcgat aaaggagcgg gtaacctcct cgaccgggag ggttgcccgc
     2401 cccgaacccg gggatcttgg agagaagatc cgagagtgcc tggaagaccc ctcgcggttc
     2461 agggaggggt gcaggtcttt tgcggcggag tacgac
//
LOCUS       DATGDI010000262         1708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_145154, whole genome shotgun sequence.
ACCESSION   DATGDI010000262 DATGDI010000000
VERSION     DATGDI010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145154"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            465..749
                     /locus_tag="VKO45_09780"
     CDS             465..749
                     /locus_tag="VKO45_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA06199.1"
                     /translation="MHSQRDLSQYALNPDELREKKKELLRLGGTRDQIIKALKSAILK
                     PDQREQFKKALSLYDTKIRKLRDQIIASDLLGNLNPDEVNIPTHYQDRKR"
     gene            853..>1708
                     /locus_tag="VKO45_09785"
     CDS             853..>1708
                     /locus_tag="VKO45_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA06200.1"
                     /translation="MRHRTAGKITDNLWYLGREESGVYLLEGKDGSIMINGGLAHILP
                     DVRKQMKEFGIDPAKIAKILILHSHFDHIGVIPWFKRSWPAIEVYGSAETWRILQLQK
                     AIDIANQFSKIVADKMGLGAALRDVDFAWRADIAGKTVAEGDRIDLGGTALNILYTPG
                     HTNCSITAYEPDRKALFASDGGGIPYREGSFASANTNFTQYMESLERMKPLVVDYLCA
                     DHYGYVTGEDARGFIDLTFREGKKLRAMMEGILREKGNVDEAAKAMNVLFYQECPDYF
                     ISADILEGV"
BASE COUNT          390 a          533 c          502 g          283 t
ORIGIN      
        1 caccttcacg atctcgccca cctcgaagac cttcaccgac aagggaggca agggaaatgt
       61 cagcgtgacg acgggcgccg gctgctcctg gacggcctcg ccaggctctg actgggtgaa
      121 attcacgtcg ggcacctcga aaactggcag taggactgtc acctactccg tcgacaccaa
      181 tttaaccaac accatgaggg cgacatcgat cagtattacc gcaacgggaa cctcgtcggc
      241 ggcaggatcc gtatccatca tcgagaaggg cagacggcgc tgatttcact ctcgtcggcg
      301 tgacggcaaa tgaaacaact tcggcggccg gaacgggaac ctcccgatcc ggccgcattt
      361 ttcatttaag cgccgggacc gttgagccga tatctaggag aggccgagac gatccggctg
      421 cattcaggga tactttcgtc ccgatatcaa gcggaggcgg caccgtgcac tcgcagcgtg
      481 acctgtccca atacgccctg aaccccgatg agcttcgaga gaagaagaag gaactcctgc
      541 gcctcggcgg tacgcgggac cagatcatca aggccctcaa atcggccatc ctgaagccgg
      601 atcagcgtga gcagttcaag aaagccctct ccctgtacga cacgaagatc cggaagctgc
      661 gcgaccagat catcgccagc gatcttctgg gcaacctcaa ccccgacgag gtcaatatcc
      721 ccactcacta ccaggaccgg aaacgctagc cggcccctcc cgcctttccc cttgcgatca
      781 cccgcccgga tgcattatag agtcaaaggg tctgtccagt acatccatca agaaaaaagg
      841 gagggtcgct cgatgaggca tcgcacggcg ggaaagatca cggacaatct ctggtacctg
      901 ggacgcgagg agtcgggggt ctatctcctc gagggcaagg acggctcgat catgatcaac
      961 ggggggcttg cgcacatcct gcccgacgtc cgaaagcaga tgaaggaatt cgggatcgac
     1021 ccggcgaaga tcgcaaagat cctgatcctg cactcgcact tcgaccacat cggggtcatc
     1081 ccctggttca agcgcagctg gccggccatc gaggtctatg gctcggcgga gacctggagg
     1141 atcctccagc ttcagaaggc catcgacatc gccaaccagt tcagcaaaat cgtggcggac
     1201 aagatgggcc tcggggcggc tctccgggac gtcgatttcg cctggcgcgc cgacatcgcg
     1261 ggtaaaaccg tggccgaggg ggaccggatc gacctgggag ggaccgcgct gaacatcctg
     1321 tacacgccgg ggcacacgaa ctgctcgatc acggcctacg aaccagaccg gaaggcactc
     1381 tttgcctccg acggaggcgg catcccctac cgggagggct ccttcgcctc ggcgaacacc
     1441 aacttcaccc agtacatgga gagcctcgaa aggatgaagc cccttgtagt ggactacctc
     1501 tgcgccgacc actacggcta tgtcacgggc gaggacgccc ggggcttcat cgacctgacc
     1561 ttccgcgaag ggaagaaact gcgcgccatg atggagggga tcctcaggga gaagggaaat
     1621 gtcgacgagg ccgcgaaggc catgaatgtc ctgttctacc aggagtgccc ggactacttc
     1681 atctcggcgg acatcctcga gggggtct
//
LOCUS       DATGDI010000263         1682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_348762, whole genome shotgun sequence.
ACCESSION   DATGDI010000263 DATGDI010000000
VERSION     DATGDI010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348762"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VKO45_09790"
     CDS             complement(<1..341)
                     /locus_tag="VKO45_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HMA06201.1"
                     /translation="MRPTTSAITRSNWFDHFLARWGVNRMGHTVEPGLYRLGSPTPES
                     EVFVSANYTLSFDALRSALEGRDAYILVLDTGGINVWCAAGKGSFGSEELVRRIGLSG
                     LAEVVSHRKLIL"
     gene            complement(503..775)
                     /locus_tag="VKO45_09795"
     CDS             complement(503..775)
                     /locus_tag="VKO45_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HgcAB-associated protein"
                     /protein_id="HMA06202.1"
                     /translation="MIKKNSSEAKCDSCGPGQGCRVEAVLSVDERGQMVLPKDVRERA
                     GIQTGEKLALISCEREGKVCCLALIKAGELSGMVKGILGPLFGEQV"
     gene            891..1445
                     /locus_tag="VKO45_09800"
     CDS             891..1445
                     /locus_tag="VKO45_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HMA06203.1"
                     /translation="MPPGAVRSIRDLIFWQYAKLIARSAGIEGRAFQMSRFTALRDGK
                     IQWSSTIREWLHEHEKPDECIYCGSKGILTTEHILPRSRGGPDHPDNAIRVCMPCNLK
                     KGDNRLYEWMGLERKDDVPRIAEGKYLKLLYELHDERGTLGIDKKELTGRLCPICDLG
                     GRCEEADTVGKLTVYCLEGCFQGK"
     gene            complement(1468..>1682)
                     /locus_tag="VKO45_09805"
     CDS             complement(1468..>1682)
                     /locus_tag="VKO45_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMA06204.1"
                     /translation="ALQCSHCVEYHMQAATKKGATREEILEVILIAGLTANSVVLAEA
                     YRVMEAQLPECGASCDTNGFGEGKKD"
BASE COUNT          330 a          528 c          494 g          330 t
ORIGIN      
        1 aggatgagct tccggtggct gacgacctct gcaaggccgg aaaggccgat gcgacggacg
       61 agctcctccg aaccgaagct gcccttcccg gctgcgcacc agacattgat cccgcccgtg
      121 tcgaggacca ggatgtatgc atccctcccc tcgagggcag accgcagcgc atcgaagctc
      181 agggtgtagt tcgccgaaac gaagacctct gactccggcg tcgggctgcc gagccggtag
      241 aggcccggtt cgacggtgtg acccatccgg ttcacccccc agcgggcgag aaaatggtcg
      301 aaccagttgg accgggtgat cgcactcgtg gttggacgga tctgagcggg tcccccggtc
      361 gccgtcgcgg cgatagtgtt ccgcccttcc ccctcattgg ctgtgggggc gcagcagggg
      421 gatttttcct ccgggggact ccccccgcag caggtacatg acgatgcagt ctgtccatcg
      481 tttttcacgg aaccggcctc cgtcacacct gctcgccgaa gagcggcccg aggatcccct
      541 tcaccatgcc ggagagctct cccgccttga taagggcgag gcagcagacc ttcccttccc
      601 tttcgcagct gatgagggcg agcttctcac ccgtctggat cccggccctc tccctcacgt
      661 ccttggggag caccatctgc ccccgttcgt cgacagaaag cacggcctcg acccggcagc
      721 cctgcccggg cccgcaggag tcacacttgg cctcgctcga atttttcttg atcatgagaa
      781 ccagatctta tctttatttt ctgattattt aatttttact gataaatcag aattgtttga
      841 atcaccagac ctgcacaatc ttcatctcgc cggggaccca gcatctctgt atgccacccg
      901 gcgcggtgag aagcattcgg gacctcatct tctggcagta cgcaaagctc atcgcgcgat
      961 cggcaggaat cgagggccgg gccttccaga tgagccggtt caccgccctc cgggacggga
     1021 agatacagtg gtcctccacc atccgagaat ggcttcacga gcacgagaag cccgacgagt
     1081 gtatctactg cggctcgaag gggattctca ccacagagca tatcctgccc cgttcccggg
     1141 gaggacccga tcatcccgac aacgccatcc gggtttgcat gccctgtaac ctgaaaaaag
     1201 gggataatag gctctatgaa tggatggggc tagagaggaa ggacgatgtc ccgaggatcg
     1261 ccgaagggaa gtacctgaag cttctctacg agcttcacga tgagcgggga accctcggga
     1321 tcgataagaa ggaactcacg ggccgcctct gtccgatatg tgacctgggt gggcgctgcg
     1381 aagaggcaga tacggtgggg aaactgacgg tttactgtct tgaggggtgc ttccagggaa
     1441 agtaatccag cccgaagaaa acaagattta atccttcttc ccttccccga acccgttcgt
     1501 gtcgcaggag gccccgcact ccgggagctg ggcctccatg acccggtacg cctcggcgag
     1561 gacgacggaa ttcgccgtga gccctgcgat gaggatcacc tcgaggatct cctcccgggt
     1621 cgcccccttc ttcgtggccg cctgcatgtg gtattccacg cagtggctgc actggagggc
     1681 cg
//
LOCUS       DATGDI010000264         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanomicrobiales archaeon isolate SMAG_U9670
            k141_203389, whole genome shotgun sequence.
ACCESSION   DATGDI010000264 DATGDI010000000
VERSION     DATGDI010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965241
            Sequence Read Archive: SRR5512136
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomicrobiales archaeon (soil metagenome)
  ORGANISM  Methanomicrobiales archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:55:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,964
            CDSs (total)                      :: 1,917
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 47
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Methanomicrobiales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203389"
                     /isolate="SMAG_U9670"
                     /isolation_source="soil"
                     /db_xref="taxon:2184052"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.1295 N 102.74125 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1938
                     /locus_tag="VKO45_09810"
     CDS             <1..1938
                     /locus_tag="VKO45_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HMA06205.1"
                     /translation="ELLENPGKVIEDVLDAIKNGQLIRTRDGKEPKGINIRFTNLPKK
                     TLIRNIRSDDINTFVSVEGILRKTTEVRPRIVEAALRCTSCGKITTVSQTYGKFQEPD
                     FCPSCEKKARMDIVASRSRFTDSQKLRIQESPEGLRGGEQPQTLDVDVTDDLTGRVAP
                     GDRVSINGILRTIQRVTAGVKGTLFDIYLECNSIEISEKEFEEVSISEEDERTILTLS
                     KDTQLLRKIIRSIAPTIYGNEDVKEAVALQLFGGIPKEMPDGSHLRGDIHMLLVGDPG
                     IAKSQILRYVVTLSPRGIYTSGKSSTAAGLTATAVKDEFGDGRWTLEAGALVLADMGI
                     AAVDEMDKMRTEDRSALHEAMEQQSISVAKAGITATLRCRCALLGAANPKLGRFDAYT
                     PISEQINMPAALLSRFDLIFVMMDQPDPKLDEAIAEHILKTHRVGELIVQHGKKPIPG
                     VDDEYIKQQLSPVTPDIPPALMRKYVAYAKRTCYPILSEEAKEALESYYLKLRGLADS
                     NKPVPVTARQIEALVRLGEASARIRLSDTIDASDAERVIHIVDTCLRQVAYDAETGSF
                     DIDKLVTGVPKSSRDIIRSIKEVIRDLGGESREARVDQVIDIVTGQGFTKDKVEQMIK
                     MLLRDGEALEPRGGIIKLIGY"
     gene            1957..2313
                     /locus_tag="VKO45_09815"
     CDS             1957..2313
                     /locus_tag="VKO45_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase family protein"
                     /protein_id="HMA06206.1"
                     /translation="MVTDRERAAFEAGIKLGALYHQWVGTPVSPATAGFIEQAIERAV
                     SLQPTVESVRVRLNRNLMSPNSYGYSELAGLMFDVEITTRLGKTRCRATLARSEDYPL
                     MSLVSCDETPPDPGHR"
     gene            2279..>2769
                     /locus_tag="VKO45_09820"
     CDS             2279..>2769
                     /locus_tag="VKO45_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HMA06207.1"
                     /translation="MTKPLPIPVTDDHIHIDPVNGRGLEAAKEFQRAGGTHIFLVSKP
                     SWSFGITPSKGEDFLPVFEKTLSIAREIRSLGLVVYPVLGVHPAEITRLGEVMDLARV
                     EEIMKGGLEVAAGLVARGDAVALKSGRPHYEVPVEVLGASNRILLHALALARDNGCAL
                     QIHA"
BASE COUNT          539 a          914 c          886 g          430 t
ORIGIN      
        1 gaactgctcg aaaatcccgg caaggtcatc gaggatgtcc tcgatgcgat aaaaaacggc
       61 cagttgatcc ggacccgtga cggcaaggag ccaaaaggca tcaatatccg tttcacgaac
      121 ctgccgaaaa agacccttat ccggaacatc cgttccgatg atatcaacac cttcgtctcg
      181 gtcgagggta tcctcaggaa gaccaccgag gtccggcccc ggatcgtgga ggcggccctc
      241 aggtgcactt cctgcgggaa gatcacgacg gtctcacaga cctacgggaa gttccaggag
      301 ccggacttct gcccgagctg cgagaagaag gcaaggatgg acatcgttgc atcccggtcc
      361 cggttcaccg actcccagaa gctccggatc caggagtccc ccgaggggct acggggcggc
      421 gaacagcccc agaccctgga cgtggacgtg accgatgacc tgaccgggag ggtggccccc
      481 ggcgaccggg tgtccatcaa cgggatcctc aggaccatcc agcgggtgac cgccggggtg
      541 aaggggaccc tcttcgacat ctacctcgaa tgcaacagta tcgagatctc ggagaaggag
      601 ttcgaggagg tctccatctc cgaggaggac gagaggacga tcctcaccct ctcgaaggac
      661 acccagctcc tgcggaagat catccggtcc atcgccccca ccatctacgg gaacgaggac
      721 gtgaaggagg cggtcgcgct ccagctcttc ggggggatcc cgaaggagat gcccgacggc
      781 tcccacctcc ggggggacat ccacatgctc ctcgtcgggg acccgggaat cgcgaagtcc
      841 cagatcctca ggtacgtggt caccctttcc ccccggggga tctacacgag cgggaagtcc
      901 tcgacggcag cgggcctcac ggcaaccgcg gtgaaggacg agttcgggga cggccggtgg
      961 acgctcgagg ccggcgccct cgttctcgca gacatgggga tcgccgccgt ggacgagatg
     1021 gacaagatgc gcaccgagga ccgctcggcc ctccacgagg cgatggagca gcagtccatc
     1081 tcggtggcaa aggccgggat caccgcgacg ctgcgctgcc ggtgtgccct cctcggggcg
     1141 gcgaacccga agctcgggag gttcgatgcc tacaccccca tctcggagca gatcaacatg
     1201 ccggccgcgc tcctctcccg gttcgacctg atcttcgtca tgatggacca gccggacccg
     1261 aagctggacg aggccatcgc cgagcacatc ctgaagaccc accgggtcgg ggagctcatc
     1321 gtccagcacg ggaagaagcc cattcccggc gtggacgacg agtacatcaa acagcagctc
     1381 tcccccgtta cgcctgatat cccaccggcc ctcatgcgga agtacgtggc ctacgcgaag
     1441 cggacctgct accccatcct ctctgaggag gcaaaggagg ccctggagtc gtactacctg
     1501 aagctgcggg gtctcgcgga ctcgaacaag cccgtcccgg tcaccgcccg gcagatcgag
     1561 gccctcgtcc ggctcgggga ggcgagcgcc cggatccggc tctcggacac catcgatgcc
     1621 tccgatgccg agcgggtgat ccacatcgtg gacacctgcc tccgccaggt ggcctatgac
     1681 gccgagaccg gctcctttga tatcgacaag ctcgtgaccg gggtccccaa gtcctcgcgg
     1741 gacatcatcc ggtccatcaa ggaggtcatc cgggacctgg gcggcgagag ccgtgaggcg
     1801 agggtggacc aggtcatcga tatcgtgacc ggccagggct tcacgaagga caaggtggaa
     1861 cagatgatca agatgctcct cagggacggg gaagcccttg aaccgcgggg cgggattatc
     1921 aagctcatcg ggtactagcg gggtgatgga taacccatgg taacagaccg agaacgcgcc
     1981 gccttcgagg cggggatcaa gctcggggcc ctctaccacc agtgggtggg gacccccgtt
     2041 tcaccggcga cggccgggtt catcgaacag gccatcgagc gggccgtctc gctccagccc
     2101 accgtggagt cggtgagggt tcgtctcaac cggaacctca tgtccccgaa ctcctacggc
     2161 tacagcgagc tcgccgggct gatgttcgac gtggagatca cgacccgcct ggggaagacc
     2221 cggtgccggg cgacgctcgc ccggagcgaa gactaccccc tcatgagcct ggtgtcctgt
     2281 gacgaaaccc ctcccgatcc cggtcaccga tgaccacatc cacatcgacc ccgtgaacgg
     2341 gcgcggcctc gaggcggcaa aggagttcca gcgggccggc gggacccata tcttcctcgt
     2401 ctcgaagccc tcctggtcct tcgggatcac gccctcaaag ggggaggatt tcctcccggt
     2461 cttcgaaaaa accctctcca tcgcccggga gatccggagc ttgggcctcg tcgtctaccc
     2521 ggtcctcggg gtgcaccccg cggagatcac gaggctgggc gaggtcatgg acctggcccg
     2581 cgtcgaggag atcatgaagg gggggctcga agttgccgcc ggcctcgtcg cccgggggga
     2641 tgcggttgcc ctgaagagcg gccggcccca ctacgaggta ccggtcgagg ttctcggggc
     2701 gtcgaaccgg atcctcctcc atgccctggc gcttgcgcgg gacaacggct gtgcccttca
     2761 gatccatgc
//
